LOCUS       DBNGZD010000001         2515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1458803, whole genome shotgun sequence.
ACCESSION   DBNGZD010000001 DBNGZD010000000
VERSION     DBNGZD010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1458803"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="AC16GM_00005"
     CDS             <1..522
                     /locus_tag="AC16GM_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR2922095.1"
                     /translation="IPVNENEVRVSLADDDLFNYPFLYMTGHGNIFFTEAEVNRLRTY
                     LTSGGFLFANDSYGLDKAFRREIKKIFPEADLVELPFDYGLYHSYFDFPNGLPKIHKH
                     DGKPAQGFGILHQGRLVVFYAYESDIGDGWEDPQVHKDPPEKREQALKMGTNLAVWAL
                     LGGKEPSKVSASK"
     gene            529..>2515
                     /locus_tag="AC16GM_00010"
     CDS             529..>2515
                     /locus_tag="AC16GM_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922096.1"
                     /translation="MANESFQKIVERLVGLDRARKLLTTQWVLASIITLLVGAFLTIL
                     VFSFLFTLPASIRIAVWLILGGTFIYLTGLRWLPMFLRRANLYRLARLVEAHFPDLEN
                     RLVAALDCQRFLQEHQPNYSPELMAAVIVAADRKTENLDFARVLDRQPLKKALRLAGL
                     TLAATFLLSLVSPANFRASFRAFLHPTEELKQSPPFQVLVSPGSVEVVKFTPVTLKAT
                     AIGQNLPRRLFVNWQNEGGQWQKELLSREALSPTANGIDTAVFTYAIREAKKNFTYFF
                     SGKNFQSEAYAITVVDIPRVTDIRATLTYPAYSRLGAVTKTDNDGNLQALTGTRVTLS
                     VETNKPVDKATLVFGDGTSKQMNVRGKTAAVPFPLTRSTTYHIQVVDHVGNANRDPIE
                     YTLTALPDAPPSVEIVRPGSDLDLSEEMQVGLEVKAQDDYGFSTMLLKYQIVSHGISG
                     AERKKELSGASVGTELIIRDRWDLANLPLVPGDLVRYWVEVADNDNISGPKWAKSKTY
                     HLRLPSLDEILSEATQKTDENISDLEKVLSGEKDLNRKLEELSRRLAKDQKLSWETKE
                     EVQKLAAEHLKFLENLNKVADQQHETAQKMAENRLAQQELVEKMQEMQKLLDEVASPE
                     LKAALRKLQQALDQLDPEQVRQALEKFQIDQEALIAKL"
BASE COUNT          581 a          757 c          678 g          499 t
ORIGIN      
        1 attccggtga acgagaatga agtccgcgtc tcgctggccg atgacgacct gttcaactac
       61 cccttcttat atatgaccgg ccacgggaac attttcttca ccgaggccga agtgaatcga
      121 ctgcggacct acctgacttc cggcgggttc ctcttcgcca acgattccta cgggctggat
      181 aaggccttcc gccgggaaat aaaaaaaatc ttcccggagg ccgatctggt ggaactcccc
      241 tttgactacg gcctctacca ctcgtatttt gattttccca acggtttgcc gaaaatacac
      301 aagcacgacg gcaaaccggc ccaggggttc ggcatcctcc accagggaag gttggtggtc
      361 ttctacgcct acgagtcgga catcggcgac ggctgggaag acccgcaggt gcacaaagac
      421 ccgcccgaaa agcgggagca ggcgctgaaa atgggcacca atctcgccgt gtgggccctt
      481 ttgggcggca aagaaccgtc caaggttagt gcgtcgaagt gagacgttat ggccaatgag
      541 agttttcaga aaatagtcga acgtttggtc gggctcgacc gcgcccggaa acttttgacc
      601 actcaatggg tgctggcgag catcattacg ctattggtgg gcgctttctt gactattctg
      661 gttttttctt ttctgttcac gctgcccgcc tctatccgca ttgcggtctg gctcatcctc
      721 ggcggaactt tcatctatct caccggcctc cgctggctgc cgatgttttt gcgccgggcg
      781 aacctctacc ggttagcgcg gctggttgaa gcacacttcc ccgatctgga aaaccgcctc
      841 gtcgccgcgt tggattgcca gcgctttttg caggaacacc agcccaatta ctcgcctgaa
      901 ctgatggcgg cggtaattgt tgctgccgac cggaagaccg aaaatctcga cttcgcccgg
      961 gtgctcgacc gccagccgct caaaaaagcg ctccgcctgg ccgggctgac tctggctgct
     1021 acatttcttt tgtcgctcgt ttcaccggcc aacttccgcg cttcattccg cgcctttctg
     1081 catccgaccg aggaactgaa acaatcaccg ccgttccaag ttttggtttc ccccggctcg
     1141 gtggaggtgg tgaagttcac gccggtaacg cttaaggcaa ctgctattgg gcaaaatctc
     1201 ccccgccggc tgttcgtgaa ctggcagaac gaaggcggcc agtggcaaaa ggaactgctc
     1261 agccgggagg cgctgtcacc aaccgcgaac ggaatcgata cggctgtttt cacctatgcc
     1321 atccgggaag cgaagaagaa cttcacctac tttttctccg gaaaaaactt ccagagcgaa
     1381 gcgtacgcga ttacggttgt cgatatcccc cgcgtcaccg acatccgcgc caccctcacc
     1441 tacccggcct acagccgcct cggagcggtg accaaaacgg acaacgacgg caacctccag
     1501 gccctgaccg gcacgcgggt cacgctttcg gtcgaaacga acaaaccggt tgataaagcg
     1561 accctcgtat tcggcgatgg aacgagcaaa cagatgaacg tgcgcgggaa aaccgccgcc
     1621 gtcccgttcc cgcttaccag gagcaccacc taccatattc aggtggtcga tcacgtgggg
     1681 aacgccaacc gcgacccgat tgagtacact ctgaccgccc tccccgatgc gccgccctca
     1741 gtcgaaatcg tccgccccgg ctccgatctg gacttatccg aagaaatgca ggtggggctc
     1801 gaggtgaaag cacaggatga ctacgggttt tcaactatgc ttttgaagta ccaaatcgtt
     1861 tcccacggca tatccggagc cgaacgcaaa aaagaactct ccggcgcttc tgtcgggacc
     1921 gagttgatca tccgggaccg ctgggatttg gcgaatttgc ctcttgttcc gggcgacctt
     1981 gtgcggtact gggtagaggt ggccgacaac gacaacatct ccggccccaa gtgggccaag
     2041 agcaaaacct accacctccg gctgccgtct ttggacgaaa tcttaagtga agccactcag
     2101 aagaccgacg aaaacatctc cgacctggaa aaagtgctct ccggggagaa ggatctgaac
     2161 cggaaactgg aggaactctc ccgccgcctg gccaaagacc agaaactctc ctgggagacc
     2221 aaagaggaag tgcagaaatt agccgccgaa cacctcaagt ttttggaaaa tctgaacaag
     2281 gtggccgacc agcagcacga gacggcccag aaaatggccg aaaaccgcct ggcccagcag
     2341 gaattggtcg aaaaaatgca ggaaatgcaa aaactgttgg acgaggtggc gagcccggaa
     2401 ctgaaagccg ccctgcgtaa gttgcaacag gccttggatc agcttgaccc cgaacaggtc
     2461 cgccaggcgt tggaaaagtt tcaaatcgac caggaggccc tgatcgccaa actcg
//
LOCUS       DBNGZD010000002         9088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_416942, whole genome shotgun sequence.
ACCESSION   DBNGZD010000002 DBNGZD010000000
VERSION     DBNGZD010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_416942"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16GM_00015"
     CDS             complement(<1..266)
                     /locus_tag="AC16GM_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922097.1"
                     /translation="MKKVIILATGWKPDYWESDKEAPYPKTSYRELPEWDVLARSCPL
                     PGLGRYYKQREDYSNNKFVYLKVTGMRFDLSSEKPYFSIKVIGQS"
     gene            complement(363..1496)
                     /gene="sucC"
                     /locus_tag="AC16GM_00020"
     CDS             complement(363..1496)
                     /gene="sucC"
                     /locus_tag="AC16GM_00020"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR2922098.1"
                     /translation="MNVHEYQAKAIFAQYGIPVPKGEVASDPDGARALAVTFGFPVMI
                     KAPVHVGGRGKAGGIKKAKDEDEAYRVASQILTMQIKGLPVKKVLVTEAVEIASEAYV
                     GIVVDRQAKKPVVMVSSAGGIDIEEVAATTPEKIVKLQVDPVLGFQPHQARQLGYALY
                     RDHVLARHAADSIAKLYHVFWEKDCSLAEINPLVTTATGKLLALDAKINFDDNALERH
                     PELAVLRDLDAGEEAEYEAREKGLSYVKLSGNIGCIVNGAGLAMATMDLVKRNGAEPA
                     NFLDVGGSSSPDKVIAAMSIILRDPNVKAILFNIFGGITRCDDIARGVKSAIETMNPR
                     VPIAIRLTGTNEDIARKILEGIGLTAVSSMTEVVKKAIELSRK"
     gene            complement(1516..2874)
                     /locus_tag="AC16GM_00025"
     CDS             complement(1516..2874)
                     /locus_tag="AC16GM_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR2922099.1"
                     /translation="MPLSRPVIEAILARGKLYEVGGCVRDRLLGFPLAEKDYDYLITG
                     IPLDDLVTLLSRFGKVDLVGRSFGVIKFTQHTGGEAATFDIALPRKEFSTGVGHKDFA
                     VSFDPQLPVEEDLKRRDFTINAMARDLARDTLIDPLAGQKDLQSRTIRMVHPKAFAED
                     PLRMLRGIQFAARFAFTLETETRAALKKNVGLITSVSAERIAEELNKLLLKAQKPSTG
                     FRLMAECGMLPLILPELAEGIGVDQPGGYHAFEVFEHSVRTVDEAAPKLVLRLAALFH
                     DVAKPRTKTVVEGGATFYNHDKLGAETTRTILTRLRYSQEITEEVCILVERHLFPTNV
                     TDKGLRRLVRRVGQNLIFDLLDLRRADVAAQGMGGKTLDVDEFERRIREELAKKPPFG
                     LRDLAVNGEVIMQEFGLAPGPKVGQVLNFLLEKVLDDPEYNVKERLLDQARIFLRKLE
                     MT"
     gene            complement(2917..3867)
                     /gene="arcC"
                     /locus_tag="AC16GM_00030"
     CDS             complement(2917..3867)
                     /gene="arcC"
                     /locus_tag="AC16GM_00030"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR2922100.1"
                     /translation="MAAPRIAVVALGGNAITRPNEEDTIPRQFANTRASLAGIIPLIR
                     EGYRLAITHGNGPQVGNAIFRVELARGKAPELPLGICVADTEGGMGYMIEQSLQNRLR
                     QEKVSRPVVTIITQVVVDKRDPSIKKPSKFIGQFYSEEEARRFERERNWVMKKDANRG
                     WRRVVPSPIPLEIVEKETIKQLVASGVIVIAAGGGGIPVYLEKDGSLEGVDAVVDKDR
                     ASAVLAHDISADFLLMLTGVDKVALNFGILTQKFLDRLTVTEAKKFLAEGHFPPGNMG
                     PKIEAAIQFLKSGGKEVIITSIPKAFEAVTGEAGTRIVPD"
     gene            complement(4126..5343)
                     /locus_tag="AC16GM_00035"
     CDS             complement(4126..5343)
                     /locus_tag="AC16GM_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014177.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIR2922101.1"
                     /translation="MKYMGSKRFMLSNGLGKLIREQLPNANRVADPFCGAGSVVYFVA
                     QNSDRQIIAGDLQKYAVVLANAVIRRSQKADTEALEKKWLKIVEKKFQRSALFSKAIE
                     LDRRYSKKVASWVKNSRRLCRTRSTIGPVWAAYGGYYFSPKQALALDYLISNLPKSSE
                     SRNLCLAAVIIAASRCAASPGHTAQPFQPTKTASKFIKVAWEMDVVKSCKNALKEIAP
                     LHAKVKGKAIVSNAKRLVSNLKKGDLVIVDPPYSGVQYSRFYHVLETIARGKCGHVSG
                     TGRYPSLSERPQSAFSNASQSLKALEELMSELAKKKTTVIFTFPKGECSNGLSGNTIM
                     EVAKKWFDIDGASRIHEHNIVTGKFSTLGGNNKLKLKNNRLKKSRIKSEELLLLLKPK
                     PKVSMLKAHESKK"
     gene            complement(5353..6207)
                     /locus_tag="AC16GM_00040"
     CDS             complement(5353..6207)
                     /locus_tag="AC16GM_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922102.1"
                     /translation="MIIDEQFRTRLAATFRPLNTVPASKTFPQAATLANSLAAEASRA
                     SASSRTVTPQLASHFGTAAVEIWQRSVHSFLISTSLTVASPIWASVCGYYSSHYSVRG
                     IAHLLGYFYLFKRKTVARLEPGPRGGYSCSFVRKPKNIPSSEHRLYWGLIKRDPIFAT
                     DPLFTENNPDVGESDVGHRNFANYIDHICRYPVFYPLDENSIRVRVDQISKFVLTDIP
                     IPRKSRFPDVEYVQLIAYHRIVCFRKILDEVLGNANRFWRVHRNPSFATGFIDFQLAQ
                     RSGLTQLN"
     gene            complement(6204..7568)
                     /locus_tag="AC16GM_00045"
     CDS             complement(6204..7568)
                     /locus_tag="AC16GM_00045"
                     /inference="COORDINATES: protein motif:HMM:NF025438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HIR2922103.1"
                     /translation="MSKTDSIQKISVIVPRNPFVQPELQTVKHCLAALDVEKLMEWWP
                     GQPHTPHRDPAKVRAIQRSLDWKRVAQIAAYLLQSEIIDAPNKLDKYFRKIYEPKKLE
                     PGREWPPKVTNVIGFERSQFPTFSNVLLHVNGAQIKPIPGRAGEAAHLIFDENNRDLR
                     FSVIDGQHRINGAYLALKIRQEQKPGEQWEIPAEVFLDKDERGAPPRHQAQIFIDVNF
                     YQKKVDRSLVADLFPTARGERGPLDEKERAQDIGRKLMLEIGPLVGMIQIPGIKFGVK
                     DVVTLATLNSAIEDVLEPMTKVNIKSLEEQTEFLAQSLDAWLEATGRKHDIVNSEKLH
                     PSNVAYQGRVLVSFLTLLPACIWTLKKMQKVLISDEAKEVLVRWLRGLMERAGLLENG
                     QFIAKEKFRKNGYLGSGGIGRFRDTLWTAAIGTRDMSKLSVEKRSHLAEVNKSKVYAQ
                     MTIS"
     gene            complement(7898..8095)
                     /locus_tag="AC16GM_00050"
     CDS             complement(7898..8095)
                     /locus_tag="AC16GM_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2922104.1"
                     /translation="MLAQDKVQLLDEMARGAEDVLAYVQPLPEEVWSFKPAPEKWSIK
                     EIVGHLADVDAKMYSPRLRHQ"
     gene            complement(8107..>9088)
                     /locus_tag="AC16GM_00055"
     CDS             complement(8107..>9088)
                     /locus_tag="AC16GM_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR2922105.1"
                     /translation="PVVAVCAVRTGAGKSQTTRRVTEVLKQLKKKVVAVRHPMPYGDL
                     SKQVCQRFATLKDLDRYRCTIEEREEYEPHIARGTVVYAGVDYEKILRQAEKEADVIV
                     WDGGNNDLPFYRPDLFITVLDPLRPGHELLYFPGEANLRAADVVVINKVDTASFEEAE
                     AVRRNVKQVNPKAKMIEAASPIFVDDSDIISGKRVLVVEDGPTLTHGGMTYGAGIVAA
                     EKFGAREIIDPRPYVTGTLKETFAAYPHIGKLLPAMGYGKIQVRDLERTINKVPAEVV
                     VIATPVDLRRIVKIRKPAVRVRYELQEIGKPDLKDVITAMLARTKKGRKG"
BASE COUNT         2039 a         2508 c         2171 g         2370 t
ORIGIN      
        1 gactgaccta tcaccttaat tgagaaatat ggtttctcgc tcgataggtc gaatctcatt
       61 ccagtaacct ttaggtacac gaatttgtta tttgagtagt cttcccgttg cttataatac
      121 cttcccaatc caggcaaagg acaactacgt gccaaaacat cccactcagg aagttcccta
      181 taacttgttt tagggtaggg agcttcctta tccgattccc aataatccgg tttccaacct
      241 gttgcgagta ttatgacttt cttcataata tttggtttaa gtagtctgat ttttattgtc
      301 ttcgaccctt actatgttat ttcgcttgta agataataca ttttactctc cttcccttgc
      361 tctcacttcc tgctcagctc aatcgccttc ttcaccactt ccgtcatcga cgacaccgcg
      421 gtcaggccga tcccttctaa aattttccgg gcgatatcct cattcgtgcc cgtgagccga
      481 atcgcaatcg gcacgcgcgg attcatcgtc tcaatcgcgc ttttgacgcc ccgggcgatg
      541 tcgtcgcagc gggtgatgcc gccgaagatg ttgaagagaa tggctttgac gttcgggtcg
      601 cgcaggatga tcgacatcgc ggcaatcacc ttgtccggcg aggacgaacc gcccacgtcc
      661 aagaaattcg ccggctccgc gccgttgcgc ttgaccagat ccatcgtggc catggccagc
      721 ccggcgccgt tgacgatgca accgatgtta ccggagagtt tcacgtagga gagtcccttc
      781 tcccgcgcct cgtattcggc ctcttccccg gcgtctaaat cgcgcaggac ggccagttcc
      841 ggatgacgct ccagcgcgtt gtcatcgaag ttgattttcg catccagcgc caggagtttg
      901 cccgtcgccg tcgtgaccaa ggggttgatt tccgccagag aacagtcttt ttcccagaaa
      961 acgtggtaga gtttggcaat gctatcggct gcgtgacggg cgagcacgtg atcacggtaa
     1021 agcgcgtacc ctaattgccg ggcctggtgc ggctggaacc ccagcaccgg atcgacttgg
     1081 agtttcacga ttttctccgg cgtggtggcg gctacttctt caatgtcgat ccccccggcg
     1141 gacgacacca ttaccaccgg tttcttggcc tggcggtcaa cgacaatgcc gacgtaggcc
     1201 tcgcttgcga tttccaccgc ttcggtcacc agcacttttt ttaccggcag acctttgatt
     1261 tgcatcgtaa gaatttgtga ggccactcgg taggcttcat cttcgtcttt cgctttcttt
     1321 atccctcccg ccttgccccg cccccccacg tgcaccgggg ctttgatcat caccgggaac
     1381 ccgaaagtga ctgccagggc tctggccccg tccggatcgg aggccacttc tcccttcggc
     1441 acgggaatgc cgtactgcgc gaagatcgct ttggcctgat actcgtggac gttcacggcg
     1501 tttctcgaaa taaagtcatg tcatctctaa tttccgcagg aaaatcctgg cttgatccag
     1561 caacctttcc ttcacgttgt actccggatc atccagaact ttctccagca aaaagttcaa
     1621 gacctgtccc acctttggtc cgggagccag cccgaactcc tgcatgatga cctcgccatt
     1681 caccgccaga tcgcgcaggc cgaagggcgg cttcttcgct aactcctccc ggatgcgacg
     1741 ctcaaattca tccacgtcga gtgtcttacc gcccataccc tgggcggcca catcggcccg
     1801 gcgcaggtca agtaaatcaa aaattaagtt ttgtccaacc cgccggacga gccgccgcag
     1861 ccctttgtcg gtgacatttg tcgggaatag atgacgctct accaagatac atacctcctc
     1921 ggtgatttct tgcgagtagc gcagtcgggt aaggattgtc cgggtcgttt ccgcacctaa
     1981 tttgtcgtga ttgtaaaaag tggctccgcc ctcgacgact gttttcgtgc gcggcttcgc
     2041 cacgtcgtgg aacaaggcgg cgaggcggag aaccagcttc ggcgccgctt catccaccgt
     2101 gcggacggag tgctcaaaaa cttcaaaagc gtggtaccca cccggctgat cgaccccgat
     2161 cccctccgcc agttccggca aaatgagcgg gagcatcccg cactccgcca tcaagcggaa
     2221 gccggtggaa ggcttttgcg ctttgagcaa aagtttattc aactcttcgg caatccgttc
     2281 tgcggaaacg gacgttatca atccgacatt cttttttaac gccgcccgcg tttccgtctc
     2341 aagcgtaaag gcaaaccgcg ccgcgaattg aatcccccgc agcatccgca gcgggtcttc
     2401 ggcgaatgcc ttgggatgga ccatccggat cgtgcgggac tgtaaatctt tctgacccgc
     2461 cagtggatcg ataagtgtat cacgagcaag atcgcgcgcc atggcgttga tggtgaaatc
     2521 gcgccgcttc aaatcttctt cgacaggaag ctgtggatca aaactgaccg caaagtcctt
     2581 gtgaccgacg ccggtggaaa attccttacg cggcagggcg atatcgaacg tggccgcctc
     2641 tccgcccgtg tgttgggtga atttgatgac cccaaacgaa cgccccacca gatcgacttt
     2701 gccaaaacgg gaaaggagcg ttaccaaatc gtccagcgga ataccggtga tcaggtagtc
     2761 atagtctttc tccgctaagg gaaaacccaa tagccggtcg cgtacgcagc cgcccacttc
     2821 gtacagtttg ccgcgcgcca gaatggcctc aatgaccggt cggctcaagg gcatagaagt
     2881 ctcgctccga caaaatgcgg gagtgtggga gagaaatcaa tccggaacaa tccgcgtgcc
     2941 ggcttcgccg gtgaccgcct caaaggcctt cgggatcgaa gtgatgatca cctctttccc
     3001 gccggatttc aaaaactgta tcgccgcttc gattttggga cccatgtttc cgggagggaa
     3061 gtggccctcg gccagaaatt ttttagcttc ggttaccgtc agccggtcga ggaatttctg
     3121 cgttaagatc ccgaagttga gcgccacttt gtccacgccg gtgagcatca aaaggaaatc
     3181 ggcgctgatg tcgtgggcca gaacggccga ggcacggtct ttgtctacca ccgcatccac
     3241 cccttccagc gacccgtctt tttcgaggta aaccggaatg cctccgcccc ccgccgcaat
     3301 cacgatgacg ccggaggcga ccagctgctt gattgtctcc ttctccacga tttccagcgg
     3361 aatgggcgac ggcaccaccc ggcgccagcc ccggttggca tcttttttca tcacccagtt
     3421 tcgttctcgt tcaaaccgcc gggcctcctc ttcgctgtag aactgcccga tgaacttgga
     3481 gggctttttg atcgaaggat cgcgcttatc gacgaccacc tgcgtgatga tggtgactac
     3541 cggccggctg accttttcct gccgcagccg gttctggagc gactgctcga tcatataccc
     3601 catccccccc tcagtatcgg ccacgcaaat ccccagaggc agctcgggcg ccttaccccg
     3661 ggccaactcc acccgaaaaa tggcgttgcc gacctgcggg ccgttgccgt gggtgatcgc
     3721 cagccggtat ccctcccgga tgagcggaat gatcccggcg agactggcgc gggtgttggc
     3781 gaactgccgc gggatggtgt cttcttcgtt cgggcgggtg atcgcgttgc cgcccaaggc
     3841 gacgaccgcg atcctgggcg cggccatcag aggtcaccgg gcttgaaagg aagcgccatt
     3901 acgcttgatt tactctaagt gcttccggtc atgcgccaac aggcgattta ggatcacctc
     3961 gccgaacgcc cagcgtccat actcttcgtg ctggggtccc tgcacaacgt tcgaaccttt
     4021 ttccccaaat ttgataatca gcttgataag gcaaagtctc agtttcctct atggaggaag
     4081 ccagtcattt gacacgaatt ttttgaattc tgcgacgctg ctgcactatt tttttgattc
     4141 atgagctttc aacatactga ccttaggctt cggcttcaga agaagaagta gttcttctga
     4201 cttaattcga gatttcttta ggcgattgtt ttttaactta agcttgttat tgcctcccaa
     4261 tgtgctgaat tttccagtaa cgatattgtg ctcatggatt ctactagctc catcaatatc
     4321 aaaccatttt tttgcgactt ccatgatcgt atttcccgat aggccattac tgcactctcc
     4381 ctttgggaac gtaaatatta cggttgtttt tttctttgct aactctgaca tcaattcttc
     4441 taatgcttta agtgattgac tcgcgttact gaaggcagac tgtggtcgtt ctgataaaga
     4501 gggatatctc cctgtaccac taacgtggcc gcatttaccc cttgcaatag tctcaagcac
     4561 atgataaaaa cgactgtatt gcactcctga atacggtgga tcgactataa caagatcccc
     4621 tttctttaga tttgacacca atcttttagc attggaaacg atggcttttc ctttaacttt
     4681 tgcgtggagc ggtgcaattt ctttaagagc atttttacat gatttgacca cgtccatttc
     4741 ccaagcaact ttaataaatt tactagcggt ctttgtgggc tgaaaaggct gtgcagtatg
     4801 tccgggagat gcagcgcatc ttgaggcagc gatgatgact gcagccaaac aaagatttct
     4861 tgattctgaa ctctttggaa gattacttat aagatagtcg agcgctaacg cttgtttagg
     4921 actgaaatag tatcctccgt aagcagccca aacaggtcca atggtcgatc tagtcctgca
     4981 caatcttcga gaattcttga cccaagacgc tacttttttt gaatatcgcc tatccagttc
     5041 aatggcttta gaaaaaagag ctgatctttg aaatttcttc tcaacaatct taagccattt
     5101 tttctccaaa gcttcagtat ccgctttttg gctacgcctt atgaccgcat ttgcaagaac
     5161 caccgcatac ttttgcaaat caccggcaat aatttgcctg tccgaattct gtgctacaaa
     5221 ataaacaact gacccggctc cgcaaaaagg gtcagctaca cgattagcat ttggaagttg
     5281 ctcccggatc aattttccga ggccattgct cagcataaac ctttttgaac ccatgtattt
     5341 catcggcaat tctcagttca gttgagtaag gccacttctt tgggctaatt ggaaatctat
     5401 aaagccggta gcaaaagaag ggtttctatg tacacgccaa aacctattag cattacctaa
     5461 cacctcatcc agaatttttc gaaaacagac gattctgtga tatgcaatta actgtacata
     5521 ttctacatcg ggaaatctgc tttttctcgg aatgggtatg tcggtaagca caaattttga
     5581 aatttggtcc acccgtactc ttatagagtt ttcatctaat gggtaaaaga caggatatct
     5641 gcaaatgtga tcaatataat ttgcaaaatt cctgtggccg acatcagact cacctacatc
     5701 ggggttgttt tcggtaaaaa gcggatctgt ggcaaaaatt ggatctcgtt ttatcaagcc
     5761 ccaatataat cgatgctcac tactgggaat atttttaggc ttccgcacaa atgaacaaga
     5821 gtaaccgccg cgtggtcccg gttctagtcg agctacggtt ttgcgtttaa atagataaaa
     5881 ataaccgagc aagtgcgcta ttccccgcac cgaataatgg ctggaataat aaccacagac
     5941 tgacgcccaa ataggactcg caactgttag agatgttgaa atcagaaaac tatggacgct
     6001 tcgctgccaa atttcaacag ctgcagttcc gaagtgtgat gctagttgtg gagtcacagt
     6061 ccgagatgac gcactagctc gagatgcttc cgccgcaaga gaatttgcca gcgtggctgc
     6121 ctgcggaaat gttttactag ctggtacggt atttaatggt cggaaagttg cagccagtcg
     6181 ggtccgaaat tgttcatcaa taatcatgat atcgtcatct gtgcatatac tttagatttg
     6241 ttgacttcgg caagatgact cctcttctcc acagataatt tagacatatc tcgtgttcca
     6301 attgcagctg tccaaagcgt atctcgaaat cttccaatgc caccgctacc aagatatccg
     6361 tttttccgaa acttctcttt cgcaataaat tgcccattct ccaatagacc agcgcgttcc
     6421 attaaacctc gcaaccatct tacgagtact tctttcgctt catcggaaat aagaaccttt
     6481 tgcatcttct ttagtgtcca gatacatgca ggtagaagtg tgaggaatga tacaagtaca
     6541 cgaccctgat aagcgacatt gcttggatga agtttttcgg aattgacaat atcatgcttg
     6601 cgtcccgtag cctctaacca agcgtccaaa gattgtgcaa ggaattccgt ctgttcttcc
     6661 aggcttttta tattcacttt ggtcatcggt tctaaaacat cttcaattgc cgaattcagt
     6721 gttgctaatg tcacgacatc cttcactcca aacttgatcc caggaatctg gatcattcca
     6781 actaggggcc ctatctcaag cataagtttt cttccgatgt cctgggcacg ttccttttca
     6841 tcaagtggac cccgttcccc tcgagcagtt ggaaaaaggt cagccacgag ggaacgatcc
     6901 actttttttt ggtagaaatt cacgtcaatg aatatttgcg cctgatgtcg tggtggtgca
     6961 ccacgttcat ctttatcaag gaatacttcc gccggaattt cccattgttc gcctggcttt
     7021 tgctcttggc gtatcttcag tgctaagtac gcaccattga ttctgtgctg tccgtcaatt
     7081 acggagaatc tcaggtcgcg attgttttca tcaaaaatta gatgcgcggc ttcacctgct
     7141 cgacctggaa tcggtttaat ctgagcacca tttacatgaa gaagaacatt tgaaaaagtt
     7201 ggaaattggc tccgctcgaa gccaattaca tttgttactt tgggtggcca ctctcgtcca
     7261 ggctcaagct ttttgggttc atatattttc cggaaatact tatcaagttt attaggagca
     7321 tcaatgattt ccgattgaag caggtaagcc gcaatctgag cgacgcgttt ccaatccaaa
     7381 gaccgctgaa tggctctgac cttggccggg tcccgatgag gtgtgtgggg ctgaccaggc
     7441 caccattcca ttaatttttc aacgtcgagc gccgcgagac aatgcttaac tgtttgcagc
     7501 tctggctgaa caaatggatt tcttgggacg ataactgaaa ttttctgaat gctatctgtt
     7561 ttggacatgg cgcaagctca actaatagtt atagacacag taacgattgc accaaagcag
     7621 tctgaaaata ttcccgcgaa taaggcactg gaagagccta ttagcaagat tatattttcg
     7681 gccccgataa gccttaggct cttcgctcaa aagctcgatc attactattg atcctccttt
     7741 ctctactctc gatccacgct taaaatatgc gtgggcggta agactggcaa tattaagagc
     7801 ttagctccct ttgatggaga gagggtgatt ttttcgttcc ccaaagaagc ggtcgacgag
     7861 ttgagaaaaa tattgaagaa ggtgtacaac gtgaatctca ctgatgccgc agccgcgggc
     7921 tgtacatttt agcgtccacg tccgctaagt ggccgacgat ctctttgatc gaccacttct
     7981 ccggcgccgg tttgaaactc cagacctcct cggggagagg ctggacgtag gccaatacgt
     8041 cctccgcccc gcgcgccatt tcgtccagta actgaacttt gtcctgcgcc agcatcggtg
     8101 ctcctttcaa ccttttcgac ctttcttcgt gcgggccagc atggcggtaa taacgtcctt
     8161 caaatcgggc ttcccgattt cctgcagttc ataccgcacc cgcaccgccg gcttgcggat
     8221 tttgacgatc cggcgcaggt ccaccggcgt cgcgatgacg accacctccg ccgggacttt
     8281 gttaattgtc cgctccaaat cgcgcacctg aattttgccg tagcccatcg ccggcaaaag
     8341 tttcccgatg tgcgggtagg cggcgaacgt ttccttgagt gtgccggtga cgtacggccg
     8401 ggggtcaatg atctcgcgcg ccccgaattt ttccgccgcc acgatccccg cgccgtaggt
     8461 catcccgccg tgggtcaagg tcggcccgtc ttcgacgact aatacccgtt tgccggaaat
     8521 gatgtcggaa tcatcgacga aaatcggcga cgccgcttcg atcatcttcg ccttcggatt
     8581 gacctgcttg acgttgcgcc gcaccgcctc ggcttcctcg aaactggcgg tatcgacttt
     8641 gttgatcacc accacgtcgg cggcgcgcag gttggcttcg cccggaaagt acaagagctc
     8701 gtgccccggg cgcaggggat cgagcacggt aatgaaaaga tcgggccggt agaacggcag
     8761 gtcgttgttc ccgccgtccc agacgatgac gtcggcctct ttctcggcct ggcgcaggat
     8821 tttctcgtaa tcgaccccgg cgtagaccac cgtcccgcgg gcgatatgcg gctcgtactc
     8881 ctcgcgctct tcaatcgtac accggtagcg gtccaagtct ttgagcgtgg cgaagcgctg
     8941 gcagacctgc ttggagagat cgccgtacgg catcgggtgc cggaccgcca ccactttctt
     9001 cttcaactgc ttcagcactt cggtgacgcg acgcgtggtc tggctcttac ccgccccggt
     9061 ccgcacggcg cagacggcca ccaccgga
//
LOCUS       DBNGZD010000003         2285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_833754, whole genome shotgun sequence.
ACCESSION   DBNGZD010000003 DBNGZD010000000
VERSION     DBNGZD010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_833754"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..916)
                     /locus_tag="AC16GM_00060"
     CDS             complement(170..916)
                     /locus_tag="AC16GM_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR2922106.1"
                     /translation="MPKVFLLIDSRVWQLVVDADPVAKGVLAILAFISVLSWAVIINK
                     IRVFRRARSESEQFLASFRRRRRIADVYANLVSLRHTPLARIFAEGFKDLEHLQRKEA
                     RNPGPVSIVRGEAVRQAAPPEDELLGGSQIDALRLTLSRVAGEELARYEKNVTVLATT
                     GNVAPFFGLLGTIWGVMDAFLSIGIRGQANLAVVAPGIAEALIATIVGLAAAIPAVMG
                     YNYCVSRLRELSGQMDNFTLELLTAFLKEQ"
     gene            1247..1423
                     /locus_tag="AC16GM_00065"
     CDS             1247..1423
                     /locus_tag="AC16GM_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922107.1"
                     /translation="MKKFLAVLLIGLVGLVVIAGCGKKEEKTAPATETAAPAPTDTTH
                     AMDTTKAADTTKTK"
     gene            complement(1989..>2285)
                     /locus_tag="AC16GM_00070"
     CDS             complement(1989..>2285)
                     /locus_tag="AC16GM_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922108.1"
                     /translation="KTVVVVITTIRGDTCQVPTADLAPGEERLLTNLVCGLPATPSDL
                     LYAKSNRGNPDLNLAVQFFCSPTLTSWGLIVLGTLLLLTLTYVYFRRRKVVRSG"
BASE COUNT          515 a          678 c          637 g          455 t
ORIGIN      
        1 tccttctgta ggggcgaacc cccgtgttcg cccgcgtctc tgattatttg ggggtgaatc
       61 cgcctcaggc ggattcatcc tgtttcttgg ggcgaatcta aagattcgcc cctaccacac
      121 cgtgggcgcg gcgccctcgc cgcgcactcc aaataatgcc tgatggcact cactgctctt
      181 tgagaaacgc cgtcagcaac tccagcgtaa aattatccat ctggccggag agctcccgca
      241 gccgggagac acagtagtta taccccatca cggccggaat cgccgccgcc agcccgacga
      301 tggtggcgat gagcgcctcc gcaatgccgg gagcgacgac ggccaggttc gcctgccccc
      361 ggatcccgat cgaaagaaac gcatccatca cgccccagat ggtccccaga aggccgaaga
      421 atggcgccac gttgcccgtg gtggcgagaa cggtgacgtt cttctcgtag cgcgccaact
      481 cttcgcccgc cacccgcgac agggtcagcc gcagggcgtc aatttgactc ccccccagaa
      541 gttcgtcctc aggaggagcc gcttggcgga ccgcctcgcc gcgcacgata gagaccgggc
      601 cgggattgcg cgcttccttc cgctgcagat gttccaaatc cttgaaccct tcggcaaaga
      661 tgcgcgccag aggcgtatgc cggaggctga ctaaattggc gtagacgtcg gcaatccgcc
      721 ggcggcggcg aaacgaggcc agaaactgct ccgactcgct tctggcccgg cggaaaactc
      781 tgattttatt gatgatcacc gcccaggaga gcaccgaaat gaaagccaga atggccagga
      841 cccccttggc caccgggtcg gcatcgacga ctaattgcca gacgcggctg tcgatcaaaa
      901 gaaaaacttt cggcatccct cctccttcat acgagtcgtt cggacggaaa ggcggtattt
      961 tcttgtcggt caatcggttt tgaccgccgg cacgacccgc aggagacaaa ataggccggc
     1021 tctccggagc ggtcaaggcc aaagagaaaa aaggtctgca agaaaaatta tttcggaatg
     1081 ccgggaaagg gcttgacggg ctggcacgcc cctctatatt agcgccttaa ttttgtttgc
     1141 ggataacagg gatgcaaatc attccaaaag tgccaaacac gcactgcggg tcgaatatga
     1201 ctcccaaggg tcgtatcgac cgtgccgggg gaggtgagag taaaggatga agaagttttt
     1261 ggccgttctt ttgatcggct tggtcggtct ggtcgtcatc gccggctgcg ggaagaaaga
     1321 agagaaaacc gcgcccgcca ccgagaccgc cgcgccggct ccaacggaca ccacacacgc
     1381 gatggacacc actaaggccg ccgacactac gaagacgaag taactccacg acgaatctcc
     1441 gaagacgaag gcagtgagga gcagaggcac tgcctttttg tttttggtgg tgcgccacgt
     1501 ccggctggga aagatttctt aaaacacgta gcgcgggttg gtttgcgtaa cccgcgccat
     1561 gtgggaacac attttcagct gcctctattt ttttcttgta gtatttcgcg cctcctgtag
     1621 ccgaggccat acggcccgta cgggattgta tcctcggcgt ccgatagggc gtggaagtaa
     1681 gaaaggaaac ggcccgcagg gccgccgagg ttagtcggca cgacccgtat gggaaacctc
     1741 ggctacgact cacttgactt tctcgtgtgg ggcgaccgcc cccggtcgcc gccagaggcg
     1801 ggggtggaat tagaatttac tcctcccaat ccctaatcac taatccctga tccctgcctt
     1861 tctgggggag gggtcaaatt aaaacggggc tgaccacatc ccatagtcaa ccccattctc
     1921 tttctcacag acaatgccct gacttccagt tccctccttt tcttctcaca ccgcttggtg
     1981 agtccgcctc agccggaccg aaccaccttc cgccgccgga agtagacgta ggtcagcgtt
     2041 aagagcaaaa gcgttcccag gacgatgagg ccccaacttg taagagtggg gctacagaaa
     2101 aattgtactg caagattcag gtcggggtta ccacgattgc tctttgcgta caacaaatca
     2161 cttggggtag ctggcaatcc acaaacgagg ttggtcagta gtctttcctc tccaggagcc
     2221 aaatcagccg ttggcacttg gcaggtatca ccgcgaatag tggtgataac aacaacaacc
     2281 gtctt
//
LOCUS       DBNGZD010000004         3883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_833846, whole genome shotgun sequence.
ACCESSION   DBNGZD010000004 DBNGZD010000000
VERSION     DBNGZD010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_833846"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..975
                     /gene="sfsA"
                     /locus_tag="AC16GM_00075"
     CDS             226..975
                     /gene="sfsA"
                     /locus_tag="AC16GM_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR2922109.1"
                     /translation="MRFEKRLLRARFIARPNRFTVKAWLQGKPILAHLHDPGRLKELL
                     FSGAKLLLEPTKGDRKTNFDVRLVETKEKNLVSLYSTYPNLLLSESWNLPLVKKIFGP
                     VSVLPEKQLGKSRIDFLLQNGKQRIWVEVKSVTLVENGVALFPDAPTARGRRHLEELI
                     QAKKSGDRAVVLFVAQRSDAKVVMPNVRTDPAFARTLARALKAGVEMYAFRCRIDRRG
                     IVLDRAIPIRLPDRRNFLATAGVTKITSKTE"
     gene            978..1463
                     /locus_tag="AC16GM_00080"
     CDS             978..1463
                     /locus_tag="AC16GM_00080"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR2922110.1"
                     /translation="MADQVEKSPEVMSRRSFFATLTKLSGGFIAVLLGIPIVGNIISP
                     FFQKPETDWIDLGNADDLHGMAPLKIPYSVDVQDGWYKTKADRFVYVVWRNDDFLVLS
                     PTCSHLGCSVRWDGRKFACPCHGGTYDREGKVTGGPPPKPLAHFATKIESGRLMAKEV
                     V"
     gene            1540..2874
                     /locus_tag="AC16GM_00085"
     CDS             1540..2874
                     /locus_tag="AC16GM_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR2922111.1"
                     /translation="MKRITSWLDKRFGLGWLIKKEWYKPLPPSTNWLFSLGSVALFLF
                     VVQILTGIFLALYYVPTPDHAYESVQFINEKVIFGKLLRGIHSWGAGLLMIILPLHLL
                     RVFFYGAYKKPRETTWMAGVLLLGVTFTFAFTGYLLPWDEKGFWATVVGTNIAGSPPV
                     LGKYVATWLKGGVTFGALTLSRFYAIHVLLLPATFLVLVSVHLYLVLKHNTAGHWDPQ
                     KNDFSRGFPFYPIQVGRDALAALVVLAIVGVLAFVKPPHLLDVANPSNTSFIPRPEWY
                     FLFHFQLLKYFPGSSRVIATVILPTLFFLALILLPFYDRRPRRSPLKRPVALTLASLT
                     VVSVITLTVLAIQEDVRLSGFGPKVPLNAVQKAGLEVFNTYGCKMCHGEGGYPGSQGP
                     SLIDATTRHDQTWLMQHFKDPQSLVPGSKMPKFDYLSDEELAQLLAYLETVK"
     gene            2951..3505
                     /gene="dcd"
                     /locus_tag="AC16GM_00090"
     CDS             2951..3505
                     /gene="dcd"
                     /locus_tag="AC16GM_00090"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR2922112.1"
                     /translation="MPIKSDRWIREMAKKHKMIDPFVDRQVRQGTISYGLSSYGYDIR
                     VASDFAIFTNVNSNFVDPKKFDRGSLVPVKAKTILIPPNSFALARTVEYFRIPRNILT
                     ICLGKSTYARCGIIVNVTPFEPEWEGHATLEISNTTPLPAKIYAREGIAQIIFLESDE
                     ICEVSYKDKKGKYQAQRGVTLPRI"
     gene            3638..>3883
                     /locus_tag="AC16GM_00095"
     CDS             3638..>3883
                     /locus_tag="AC16GM_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922113.1"
                     /translation="MRRLSLLFLAVSLVAFSAGTVMAQTTEDDMVRRFIEEKEKAQKE
                     KNKKKKKTAPQKEATAKQAETASTVDTSKNKGTGQTAG"
BASE COUNT          848 a         1198 c          997 g          840 t
ORIGIN      
        1 ggcaccggct tcggctgccg cttactcttg caacttctgc gcgactgggg gcgggggtaa
       61 catcttcgct ctggccgcta tctcttccct ccccttgagg ggagggccag ggaggggtca
      121 tattccttct ttctttgacc accgccactg tcaggttcct tacaaactga gccaactctc
      181 gctcgtattc tttccaaaac cggggaattg gtttcgagag atagaatgag atttgaaaaa
      241 cgcctccttc gagcaaggtt cattgcccgc cccaatcgat ttactgtcaa agcatggctg
      301 caaggcaaac cgattctggc ccacctccac gaccccggac gcctgaaaga actcctcttt
      361 tccggagcaa aacttctcct cgaaccgacc aaaggtgacc ggaagacaaa tttcgatgtt
      421 cgtctggtcg aaacgaaaga aaagaacctg gtctctcttt actcgactta ccccaactta
      481 cttctgtcgg agtcgtggaa cctcccgctg gtgaaaaaaa tcttcggtcc ggtctcagta
      541 ctcccggaaa aacaactggg gaaaagccga atcgatttcc tcctccagaa tggcaagcag
      601 cgtatctggg tcgaggtcaa gtcggtgacc cttgtagaaa acggcgtggc gctcttcccg
      661 gatgccccca cggcacgcgg ccgccgccac ctcgaagaat taatccaagc gaaaaaatca
      721 ggcgatcggg ccgtcgtctt gttcgtcgcc cagcgctccg acgccaaggt ggtcatgccc
      781 aacgtccgca ccgacccggc cttcgcccgc acactcgctc gcgctctaaa agcgggagtt
      841 gaaatgtacg cctttcgctg tcggatagat cgcagaggaa ttgtgctgga tcgcgccatt
      901 cccatccggc tgccggaccg aagaaacttt ctggcgactg caggggttac aaaaattaca
      961 tcgaagacgg agtaactatg gcagaccagg tcgaaaaatc tccggaagta atgagccgcc
     1021 gctcattttt cgccaccctg accaaactct ccggtggctt cattgccgtg ctcctgggaa
     1081 tcccaattgt gggcaatatt atctcgcctt ttttccagaa accggaaacc gattggattg
     1141 atttaggcaa cgctgacgac ctgcacggca tggcgccctt gaaaatcccc tactcggtcg
     1201 atgtgcagga cggctggtac aaaacgaagg ccgaccgctt cgtctacgtg gtctggcgca
     1261 atgacgattt tttagtcctt tcgccgacct gcagccacct gggctgctcg gtgcgctggg
     1321 acggccgcaa attcgcctgc ccctgtcacg gcgggacata cgacagggaa gggaaagtta
     1381 ccggcgggcc gccgcccaaa ccactggcgc actttgccac gaaaatcgaa agcggccgcc
     1441 tgatggcaaa ggaggtggtg tgaccctcat ccgacccggc gcaaatcttc tgattggagg
     1501 acgacggagg gcactcgccc cacgcgcaga cactcctcta tgaaaagaat cacctcttgg
     1561 ttagacaagc gcttcggcct gggctggctc atcaaaaaag agtggtacaa gcccctccca
     1621 ccttccacca actggctctt cagtctgggg agcgtggcgc tctttctctt cgtcgtccag
     1681 atcctcaccg gcattttctt agcactctac tacgtcccca cacccgatca cgcctacgag
     1741 agcgtccagt tcatcaacga aaaggtcatc ttcgggaaac tgttgcgtgg cattcattcc
     1801 tggggtgcgg gacttctgat gatcatcctg ccgctccacc tgttacgggt cttcttttac
     1861 ggcgcgtata agaagccgcg cgaaacgacc tggatggcgg gcgttctgct cctgggtgtg
     1921 actttcactt tcgccttcac cggatactta ttgccctggg atgaaaaggg tttctgggcc
     1981 accgtggtcg gaacgaacat cgccggctcg ccgcccgtac tgggcaaata cgtcgccacc
     2041 tggctcaaag gcggcgtcac cttcggggct ttgaccctct cgcgcttcta tgcgattcac
     2101 gttctccttc tgccggcaac cttcctcgtc ttggtttcgg tgcacctcta tctggtgctc
     2161 aagcacaaca ccgccggcca ctgggatccg cagaagaatg atttcagccg cggcttcccc
     2221 ttttacccga ttcaggtggg gcgcgacgcc ctggcggcgc tggtagtgct cgcaattgtc
     2281 ggtgttctgg cctttgtcaa accgccccac cttttggacg tcgccaaccc gtccaacacc
     2341 tccttcatcc cccggccgga gtggtatttc ctgttccact tccagttgct caagtatttc
     2401 ccaggttcgt cacgtgttat tgccaccgtc attctgccga cgctcttctt tctggctttg
     2461 attctcctcc ccttttacga ccgccgtccg cggcgcagtc cgctcaagcg gccggttgct
     2521 ttgactctgg cctccctcac ggtcgtgagc gtcatcaccc tgacggtgct tgcgattcaa
     2581 gaggacgtac gcttatccgg cttcgggccc aaggtgccgc tgaacgctgt ccagaaagcc
     2641 ggcctggagg tcttcaacac ctacggctgt aaaatgtgtc acggcgaggg cggctacccc
     2701 ggcagccagg gaccgagttt gatcgacgct actacccggc acgaccaaac ctggctgatg
     2761 caacacttca aagacccgca gtcgcttgtt cccggctcca agatgccgaa attcgattac
     2821 ctctccgacg aagaactggc gcaactcctc gcctacctgg agacggtgaa gtagatcggt
     2881 ttcggccggc tgttgacttc cccgccgacg gggcattatt aggctgcgaa aaaattacgc
     2941 cggagcaaaa atgcccatta aatctgaccg ctggattcgg gaaatggcga aaaagcacaa
     3001 aatgatcgac ccgttcgtgg accgtcaggt gcgccaggga acgatctcct acggcctttc
     3061 ctcctacggc tacgacatcc gggtggcgag cgacttcgcc attttcacca acgtcaactc
     3121 caatttcgtc gatccgaaaa aattcgatcg cgggtcgctc gtgcccgtga aggccaaaac
     3181 gattctgatt ccgcccaatt cgttcgccct ggcgcgcacc gtggaatact tccgcatccc
     3241 ccggaacatc ctcaccatct gcctggggaa atcgacctac gcccgctgcg ggatcatcgt
     3301 caacgtcaca ccattcgagc cggagtggga aggccacgct accctggaaa tttccaacac
     3361 cactcctttg ccggcgaaaa tctacgcccg cgaaggcatc gcccagatca tatttctgga
     3421 gtccgacgaa atctgcgaag tatcctacaa agacaaaaaa gggaagtacc aagcccagcg
     3481 gggagtcacc ctcccccgta tttagtccaa ggtctccttg aggttagggg agtcttaacc
     3541 tcgacacccc gacacctcga tacttcgaca ctcacttaag tttttcccct ttccgtccga
     3601 tttaatagca gatgccaaat tggacaagga ggggagtatg agacggctgt ctcttttgtt
     3661 tttggctgtg agtctggtgg ctttctcggc cggaaccgtg atggcccaga ccaccgaaga
     3721 tgacatggtc agacgcttca tcgaagagaa agaaaaagcg cagaaagaga agaacaagaa
     3781 gaagaaaaag accgcgcccc aaaaagaggc caccgccaag caagccgaaa cagcctcgac
     3841 cgtcgatacg tcgaagaaca aggggacggg tcagaccgcc gga
//
LOCUS       DBNGZD010000005         3745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1667424, whole genome shotgun sequence.
ACCESSION   DBNGZD010000005 DBNGZD010000000
VERSION     DBNGZD010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1667424"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="AC16GM_00100"
     CDS             <1..1174
                     /locus_tag="AC16GM_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HIR2922114.1"
                     /translation="TVSVRLRGVLIACVLIGVVTFAFALNVDPQRAWYNFLQEHYFFL
                     CLGLAGVFFVALQHATNSTWSVSLRRIPEGLAAYLPVALLLQIGVIIGAKYLYAWVTP
                     EFTEAHPLHYAGQPPPGGRLAYLNLPFFSIRTFAFLGVWILFGWLLVRNSIQQEETRD
                     PLLSRKNVKLSIAFLPVFALTFTLAVVDLLMSLEPRWYSTIFGVYCFSGLFQGGLAFI
                     IILVILLRRQGALAGVTNPAHLKDLGTLLFAFSVFMCYIGFSQFMLIWYANMPEETFY
                     IMKRLASGWLPVTVALPLLKFFFPFFVIMSQNAKKNEKVMLVTAIVVLVGQWLDVYWM
                     VAPAFRQGPPLFGWIEAGIAIGFLGLFGLCLVTFFRRFSLVPAGDPRLLESVHLHH"
     gene            1254..2219
                     /locus_tag="AC16GM_00105"
     CDS             1254..2219
                     /locus_tag="AC16GM_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2922115.1"
                     /translation="MEKPKEKTNPFYYFPFFILAAVLVVFVFLSGYQRGVSTGQRTPP
                     PLEKPGGQVATVDIRKLRIATPEALARGKMLFLTNCVSCHGPEGRGDGERAAELSPRP
                     RNYHTEKFKFGAAPIQIYHTSSNGSPGTSMPAFELLPPEDRWALVHFVRTQIPNPPDD
                     SPEEIAKLPGGEGGPASVTTTPVTQPTVPIPAAPRIPIALAMQKLAVPPPEPGEKLAL
                     PAHGQGAVIYNRNCVTCHGGSGEGGRKTIYLLRLKPYLYVKSKSLQEPDRPWRQNKNE
                     FMKIVTKGFPGQTMPGFGTLTRSEIEALYAYVLNFAAPKKNGNKS"
     gene            2216..2998
                     /gene="coxB"
                     /locus_tag="AC16GM_00110"
     CDS             2216..2998
                     /gene="coxB"
                     /locus_tag="AC16GM_00110"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR2922116.1"
                     /translation="MRKFFVVVSLVVPMAFLTGLSSAWAHTPLTNWLQDPATPLAREV
                     RADFWTMLAIIAPFLLGAEGLLLYAVLKFRTQPGRKPATFHENFKLEVFWTALPAVAL
                     VLIAIPAFRTMHAMNVPPKSDLVVEVIGHQFFWEYRYPRYGITLAEEPLVVPAGKVVT
                     LNCTSVDVIHSWFVPAFGVKQDANPGRITNAWFKADAPGTYRGQCAELCGKLHAQMYI
                     TVKVLPEDEYKQWLAQKIAALPVPAGAPPATAQPPTTGKGGA"
     gene            2995..>3745
                     /locus_tag="AC16GM_00115"
     CDS             2995..>3745
                     /locus_tag="AC16GM_00115"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR2922117.1"
                     /translation="MSNRYGSLWSRLRPWLVTTDHKKIGIMYLLVALFMAIFGGAFAG
                     LLRAQLATPAGKVLAPEFYNQFLTMHGTLMIFFVIIPAMVGFGNYLVPLQIGARDMAF
                     PRLNALSLWIVFPAATLMLGSFLVTGGTAQAGWTGYVPLTSRAYSGTPGVDMWILGLH
                     LVGLSSILGAINFLVTIINMRAPGMKYMKMPLFTWTWFVNATLMLVATPVLAGTITMV
                     LSDRILGTGFFRPSQGGDPILYQHLFWFYSHP"
BASE COUNT          689 a         1188 c         1014 g          854 t
ORIGIN      
        1 gacggtgtcc gtccgtctgc ggggggtgtt aattgcctgt gtcctcatcg gggtcgtcac
       61 ctttgccttt gccttaaacg tcgatcccca acgcgcctgg tacaatttcc tgcaggagca
      121 ctacttcttc ctctgtctgg ggctggccgg cgtcttcttc gttgccctcc agcacgccac
      181 gaactccacc tggtcggttt cgctgcgccg cattccggag gggctggccg cctacctgcc
      241 ggtggcgctc ctcctgcaga ttggagtaat catcggggcc aaatatctct acgcctgggt
      301 caccccggag ttcaccgaag cccaccccct ccactacgcg ggtcaaccgc cgcccggcgg
      361 ccgcctggct tacctgaact tgcccttctt ctccatccgg acttttgcct tcctcggggt
      421 ctggattctc ttcggctggc ttttggtgcg caattcaatc cagcaggaag aaacgcgcga
      481 tccgctcttg agccgtaaaa acgttaaact ctccatcgcc tttctgcccg tcttcgctct
      541 caccttcacc ctggccgtgg tcgacctctt gatgtctctg gaaccgcgct ggtactccac
      601 catctttggg gtctactgct tttccgggct cttccagggc gggctggcct tcatcatcat
      661 cctggtcatt ctcctgcggc gccagggcgc tctggccggc gtcaccaacc cggcccacct
      721 gaaagacttg ggtacgctcc tctttgcttt ttcggtcttc atgtgctaca tcgggttttc
      781 gcagttcatg ctgatctggt atgccaatat gccggaagaa actttttaca ttatgaagcg
      841 cctggcctca ggttggctgc cggtaaccgt ggcgctgccg cttttgaaat tctttttccc
      901 cttcttcgtc atcatgtcac aaaacgccaa gaaaaacgag aaggtgatgc tggtcacggc
      961 catagtggtg ctggtgggcc agtggttgga tgtttactgg atggtggcgc cggcgtttcg
     1021 ccagggacca ccgctcttcg gctggatcga agcgggcatc gccataggct ttttggggtt
     1081 attcggcttg tgtctggtga cctttttccg ccgcttttct ttggtgccgg caggcgaccc
     1141 gcggctcctg gaaagtgtcc atttgcacca ttgattaaag ttagaaataa gactatcaca
     1201 atcgccataa aggcgtactt acaatggagt aaagagaagg taaaaaccgc gtaatggaaa
     1261 aaccaaaaga aaaaacgaac ccgttctact atttcccctt tttcatttta gccgctgttt
     1321 tggtcgtctt cgttttcctc tccggctacc agcggggcgt gagtaccggc cagcgcacgc
     1381 cgccgccgct cgaaaagccc gggggtcagg tggcgacggt ggatattcgc aaactgcgca
     1441 tcgccacccc ggaggcgctg gccagaggga agatgctctt tttgaccaac tgcgtttcct
     1501 gccacgggcc ggaggggaga ggcgatggcg aacgggccgc tgaactttca ccccgtccgc
     1561 ggaattacca taccgagaag ttcaagttcg gcgccgcgcc gattcagatt taccacacga
     1621 gttccaacgg ctcacccggc acctcgatgc ccgcttttga actattaccg ccggaagacc
     1681 gctgggcgct ggttcacttc gtccggacgc aaatcccgaa tccgcccgac gactccccgg
     1741 aagagatagc caaattaccc ggcggagaag gcgggccggc ctcagtcaca acgactccgg
     1801 tgacgcaacc gacggtccca attcctgcgg ctccgcgcat ccccattgcg ctggcgatgc
     1861 agaaattggc ggtgcctcct ccggaaccgg gagagaaact tgcgcttccc gctcacggcc
     1921 aaggcgccgt aatctacaac cggaactgcg tcacctgtca cggcggatcg ggcgaaggcg
     1981 gccggaaaac gatctacctt ttgcggctga aaccctacct ctacgtcaaa tccaaaagtt
     2041 tgcaggagcc ggaccgtccc tggcgccaga acaaaaatga atttatgaaa atagtgacca
     2101 aaggatttcc gggacagacc atgcccgggt ttggaactct gacccgttcg gagatcgagg
     2161 cgctctacgc ctacgtgctg aattttgccg caccaaagaa aaacgggaat aagtcatgag
     2221 aaaatttttc gtggtggtat ccctggtcgt tccgatggcg ttcctcaccg gccttagcag
     2281 cgcgtgggcg catacaccat tgaccaattg gctccaagat ccggccacgc ctttggcccg
     2341 cgaggtgcgg gcggacttct ggacgatgct cgccatcatc gcccctttcc ttttgggtgc
     2401 cgagggactt ctgctctacg ccgttttgaa gttccgcacg cagcccgggc ggaaaccggc
     2461 cacctttcac gagaacttca aactggaagt cttctggacc gccttgccgg cggtggcgct
     2521 ggttttgatc gccatccccg ccttccgcac catgcatgcc atgaacgtcc ctcccaaaag
     2581 cgacttggta gtggaggtga tcggccacca gttcttctgg gagtaccgct acccgcgtta
     2641 cggcatcacg ctggcggagg aaccgctcgt ggttccggcc ggaaaggtgg tcaccttgaa
     2701 ctgtaccagc gtggatgtga ttcactcctg gtttgtcccg gcctttggtg tcaagcagga
     2761 tgccaacccg ggccgcatca ccaatgcctg gttcaaggcc gatgcgcccg gcacctaccg
     2821 cggccagtgc gccgagttgt gcggcaagct ccacgcccag atgtacatca ccgtcaaggt
     2881 cctgccggaa gatgaataca agcagtggct ggcccaaaaa attgcggctc tccccgtccc
     2941 tgccggagca ccccccgcaa cggctcaacc cccaactacc ggcaagggag gagcatgagt
     3001 aaccggtacg gcagtctctg gtcgcgcttg cggccctggc tcgtcaccac tgaccacaag
     3061 aagatcggga tcatgtacct cctcgtggcg ctctttatgg cgattttcgg cggcgccttc
     3121 gccggacttc tgcgggcgca gctggccacc ccggcgggca aagtgctcgc cccggagttc
     3181 tacaatcagt ttttgaccat gcacggaacc ttgatgatct tcttcgtcat catccccgcc
     3241 atggtcggct tcggcaacta tctggtgcct ttgcaaatcg gcgcccgcga catggccttc
     3301 ccgagattaa atgcgctttc cctctggatt gtcttccccg ccgccacttt aatgctcggc
     3361 agttttttgg tgaccggcgg caccgcccag gccggctgga ccggctacgt ccctttaacc
     3421 tcccgcgcct actccggcac gccgggcgtc gatatgtgga ttctgggact gcacttggtc
     3481 gggctcagtt cgatcctcgg cgcgatcaac tttttggtga ccatcatcaa catgcgcgcg
     3541 cccgggatga agtatatgaa aatgcccctt tttacctgga cctggttcgt caatgccacc
     3601 ctgatgctcg tcgcgacgcc ggtcttagcc ggcaccatta cgatggtact cagtgatcgc
     3661 atcctgggaa ccggtttctt ccgccccagc cagggaggtg accccatcct ctaccagcac
     3721 ctcttctggt tctactccca tccgg
//
LOCUS       DBNGZD010000006         2592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1806518, whole genome shotgun sequence.
ACCESSION   DBNGZD010000006 DBNGZD010000000
VERSION     DBNGZD010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1806518"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="AC16GM_00120"
     CDS             <1..959
                     /locus_tag="AC16GM_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR2922118.1"
                     /translation="GVPLLFEKNLAGIWRGIGKQPFGKRTVVQILYRLVKVVKRSTGI
                     NLGIAVFSGLRRRAGLGALRMFVCGGAPLDPATAEGFDLLGIKFLQGYGLTETAPVLT
                     VNTDYKQKYASVGKPIPGVELAIINPDGRGVGEIAARGDSVMLGYYKNPKATAQVLRD
                     GWFFTGDAGWRDHEGYYYITGRLKNVIVTPGGKNVYPEEVEHHLMQSPFILEVLVHGQ
                     KVSGGEEVEATVVPDQEYFADFCSTRGGEATPETMEATLKSEIARICAHLADYKRVKR
                     FQIRLQEFDITASGKIKRRQITPAAPGVSVPAGVMATRDSSP"
     gene            1259..>2592
                     /locus_tag="AC16GM_00125"
     CDS             1259..>2592
                     /locus_tag="AC16GM_00125"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR2922119.1"
                     /translation="MFKKILIANRGEIAVRVIRACREAGTTAAVVFSDADRTSLAVRL
                     ADEAYRLGPPPVRESYLQMEAILTIAKEIGAEAIHPGYGFLAENPRFAQLVAESGLAF
                     IGPPAAAIRALGDKLEARRLMATSGVPVVPGSPEPVDQQNVEKIAAGIGYPVLLKAAA
                     GGGGKGMRVVQQPGELKSALRAAQSEARTAFGDDRIYVEKFLEKPRHIEVQIIADRFG
                     SVCALGERECTIQRRHQKVVEESPSVFVTPELRAGLFDAARQAAHASGYVNAGTVEFL
                     VDARRNYYFLEVNTRLQVEHPVTEEVTGLDLVRLQLQIASGEKLPLTQAEVRFSDAAL
                     ECRIYAEDPTNNFFPSTGRLERYREPAGLGVRVDSGVVEGDEITLYYDPLIAKVITSG
                     RDRLQAIERMRRALAEFEIAGVVTNLSLHRAILSHPEFLSGNISTDFLRAYLP"
BASE COUNT          526 a          756 c          803 g          507 t
ORIGIN      
        1 tgggagtgcc gctcctcttt gaaaaaaatc tcgccggcat ctggcgcggg atagggaaac
       61 aaccgttcgg gaagcgcacg gttgtgcaga ttttgtaccg gctggtcaaa gtggtcaaac
      121 ggtcaaccgg gatcaatctc gggatagcgg tattcagcgg tttgcgccgg agagccggtc
      181 tgggggcgct gcggatgttc gtctgcggcg gggccccgct ggatccggcc accgccgaag
      241 ggtttgacct tctgggcatc aagttcctgc agggatacgg cttgacagaa acggcgcccg
      301 ttctgaccgt taataccgac tataagcaga agtacgcctc tgtgggcaag ccgattccgg
      361 gggttgaact ggccatcatc aatcccgacg gccggggcgt cggggaaatt gcggcgcggg
      421 gcgactcggt gatgctgggg tactacaaaa accccaaggc caccgcccag gtgctgcgcg
      481 acggctggtt tttcaccggc gatgcgggct ggagggatca cgaagggtac tactacatca
      541 ccggccggtt gaagaacgtc atcgtcaccc ccggcggcaa gaacgtctac ccggaagaag
      601 tggaacacca cctgatgcaa agccccttca ttctggaggt tttggtccac gggcagaagg
      661 tatcgggcgg ggaagaagtg gaagcgacgg tggtgcccga ccaggaatac tttgccgatt
      721 tttgcagcac tcgcggagga gaagctaccc cggagacaat ggaagcaacc ttgaagtcgg
      781 aaattgcgcg catctgcgcc cacctggccg actacaagcg ggtcaaaaga tttcagattc
      841 gcctgcagga gtttgacata accgcctccg ggaaaatcaa gcgccgccag atcacgccgg
      901 ccgcgccggg agtttccgtc cccgctggag tcatggccac acgcgacagt tctccgtagt
      961 tcaggcggtc tctggatcag aggtcggagt ggaccgcctg accacgcatt ctcaatcgac
     1021 cacgtctgtc gctgaagccg tcgctaccgc cggcagcacc ggaaatgggg gtcttactta
     1081 aagtaaggaa tcataaatta accgcatctc tttatagttt atatacttat aaaaatggaa
     1141 accatcccct ctcagtctct tctacagaat aatctccagc tgctccgctg tttgacaaca
     1201 ctccgcagtt cgggtacatt tctgcgaatc gccggttttt tgctgaccta agggagtaat
     1261 gttcaagaaa atcctcattg ccaatcgcgg ggaaattgcg gtgcgcgtca tccgcgcctg
     1321 ccgggaggcg gggacgacgg cggcggtggt cttctccgac gccgaccgta cctcgctcgc
     1381 ggtgcggctg gccgacgagg cctaccgcct cgggcctccg ccggtgcggg aaagctacct
     1441 gcagatggaa gcgatcctga cgatcgccaa agaaatcggg gccgaagcga tccaccccgg
     1501 ctacgggttt ctggcggaaa atccgcgttt tgcccaactg gtggcagaaa gcggcctggc
     1561 gttcatcggc ccaccggcgg ccgcgattcg cgcgctgggc gataagctgg aagcccggcg
     1621 gctcatggcc acctccggcg ttccggttgt gcccggaagt cccgaaccgg tagatcaaca
     1681 gaacgttgag aaaatagccg caggaatcgg gtatccggtg cttttgaaag ctgccgccgg
     1741 gggcggcggc aaaggtatgc gcgtcgtgca gcagccgggt gaactgaaaa gtgccctgcg
     1801 ggcggcgcag tcggaagccc ggaccgcgtt cggcgacgac cgcatctacg tcgaaaagtt
     1861 tctcgaaaag ccgcgccaca tcgaagtgca gatcatcgct gaccgtttcg gcagcgtttg
     1921 cgccttagga gagcgggagt gcacgattca gcgccgccac cagaaggtgg tcgaggagtc
     1981 gcccagtgtt ttcgttacgc cagagttgcg ggccggactt tttgacgccg cccgtcaggc
     2041 agcgcacgcc tctggctacg tcaatgcggg cacggtggaa ttcttagttg acgcccggcg
     2101 caactactac ttcctcgaag tgaacacgcg cctccaggtg gaacacccgg tgaccgaaga
     2161 ggtgaccggg ctcgatctgg ttcgtctgca gctgcagatt gcttccggag aaaaactccc
     2221 actcactcag gcagaggtgc gtttttcgga cgcggcgttg gagtgccgca tctatgccga
     2281 ggatccgacc aacaatttct ttccttccac cggccgtctg gaacggtacc gcgagccggc
     2341 cggtctggga gtgcgggtcg attccggagt cgttgaaggt gatgagatca cgctctacta
     2401 cgatcctctg atcgccaagg tcatcacctc cggccgggat cggctgcagg cgattgagcg
     2461 gatgcgccgg gcgctggcgg agtttgaaat tgccggcgtg gtgaccaacc tctcactcca
     2521 tcgggcgatt ctctcccacc ccgaatttct ttccggcaac atctccaccg attttctgcg
     2581 tgcatacctg cc
//
LOCUS       DBNGZD010000007        17731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_487001, whole genome shotgun sequence.
ACCESSION   DBNGZD010000007 DBNGZD010000000
VERSION     DBNGZD010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 17731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17731
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_487001"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="AC16GM_00130"
     CDS             <1..1075
                     /locus_tag="AC16GM_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922120.1"
                     /translation="FEYQYNRQENRYQPESVSDVLRDTLALDLQRRQNILWNQEVRAT
                     VGKQLKNVAGVTLGWHWEKGAEDYGVNFKDQRRETGELSGTVGLRPSSSDTVAVFGSI
                     GVTSYFVPPISGFLSDRDLLNGTVSLSAGHRFSPYFDLKLTGSRRLLKQIYLSGRYSA
                     NNRTTDNYLLAPVLSYRFPGGFALRQGFNLGANYVTFDFLKTSPNADHTILRQASSAS
                     EMTLPLTARLAWILGYTYRYEDFGGLLWRDQWVEQTTWDRRTNIVDARFTYQWAKSLG
                     VSGGFIWEQKFTYDHVARLSTNTIERIRSLSFIRKTVTFGLTYALDGANFLRADYQRR
                     AQSGSGFSVGDTDWATVSYGRTF"
     gene            1077..1694
                     /locus_tag="AC16GM_00135"
     CDS             1077..1694
                     /locus_tag="AC16GM_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922121.1"
                     /translation="MPGSNFLKRPTSATAVIVSLALLVGACKNPFSTREAEKPLGQTG
                     TWSFPASPDLVLRNLLFSFNERVVANYTTCFSSDFIYAVDFFISGVPDTSRSWGNAAE
                     LELASKLFNFASAHVDSLNYVLSLAPDPSRTDIRQDTLAIFFRTYQILQLRLKPQPPE
                     TTVYAGVATFHLRQDSFNFWALDRWDDQHQSSTIPSWGDLKASFR"
     gene            1691..2242
                     /locus_tag="AC16GM_00140"
     CDS             1691..2242
                     /locus_tag="AC16GM_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922122.1"
                     /translation="MRRRLLAVIFLLLVIATACRNPFAPPEAGPARSGPLARQTDPDS
                     TLANFRYAYERRDLDVYENCLASNFVFLYIDQDLTGQIEQVAVPRDGASGDLARTERL
                     FRVFDAVSLDTWNVIAEPDDSTATGVVKRRRVNFHLSVQDLTGDFNFDEYQASGFARF
                     TFEQSKTDNLWRIVTWEDQSFTQ"
     gene            2251..2979
                     /locus_tag="AC16GM_00145"
     CDS             2251..2979
                     /locus_tag="AC16GM_00145"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR2922123.1"
                     /translation="MPKLSVNVDHVATLREARKIKEPDPVFAAMLAELAGADGITVHL
                     REDRRHIRDRDLYVLKEVVKTELNLEMAAIPQMVERALEVKPHLVTLVPEKREELTTE
                     GGLDVTGFSERIGAVLKQMKPTGIRVSLFIDPEVEQIKAAAKLRAEIIELSTARYAES
                     KTDKEIDEELKKIDQMTDMADKLGIEVHAGHGLNYRNIVPLLEIEKITEYAIGHAIVA
                     RAVLVGFSQAVKEMIQIIRERCPN"
     gene            complement(3165..3238)
                     /locus_tag="AC16GM_00150"
     tRNA            complement(3165..3238)
                     /locus_tag="AC16GM_00150"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3201..3203),aa:Ser,seq:tga)
     gene            complement(3251..3334)
                     /locus_tag="AC16GM_00155"
     tRNA            complement(3251..3334)
                     /locus_tag="AC16GM_00155"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3297..3299),aa:Ala,seq:agc)
     gene            complement(3389..4084)
                     /locus_tag="AC16GM_00160"
     CDS             complement(3389..4084)
                     /locus_tag="AC16GM_00160"
                     /inference="COORDINATES: protein motif:HMM:NF017885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR2922124.1"
                     /translation="MRHQFIPQENIERIALGDFARYKDQKGNFPLSFPLDPEDVFHTL
                     FGLTTEYIDFDQHGLAAAEGEELLGCLYPEGQFFHDDDKLILVNCTVSEQMQRFTAFH
                     EGAHYALHVWPTKNQKELFGEKKKDLAPSFCRTNQVNGKQYDPIEYQANRYAAAMMMP
                     SKEVFRVVDENRVVDLDIYGERLKKHFGVSQMAMEIRLHELGYRYANGKYGGIPKEGN
                     GWRIKVNKGRNSK"
     gene            complement(4085..4381)
                     /locus_tag="AC16GM_00165"
     CDS             complement(4085..4381)
                     /locus_tag="AC16GM_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2922125.1"
                     /translation="MHKLFGRKLRQLREAKGLGIKALAPELGIDYTHLSNLELGYKKP
                     SAELIRRIAEYFKYSEEELKLLAGYIPEDISEILAQNPVKAPEYLRKKFKEKGK"
     gene            4604..7552
                     /locus_tag="AC16GM_00170"
     CDS             4604..7552
                     /locus_tag="AC16GM_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HIR2922126.1"
                     /translation="MIGKTISHYKIIEKLGEGGMGVVYKAHDLKLDRLVALKFLPKHL
                     TSDATEKERFIHEARAASALNHPNITTIHEIDEFEGQIFIVMEFCEGKTLRQIVESET
                     LPIKKVLEVATQVCEGLALAHEKWIVHRDIKSDNIMLTARGQVKIMDFGLAKLRGRGE
                     AQTGSTLGTAAYLSPEQARGEEVDQRSDIFSFGVVLYELLAKRLPFQGEHQAALTYAI
                     IYEEPQPLTRFNNQVSAKLEDMVFKALAKDREERYQHIDDLLADLRREKKNVEYVKVP
                     HVPSAPAPPAPSKKNLWKYLVPTSAVVVLALVIFILKPFKFEVGPEQKAVAAENSLAV
                     MYFENIPDPEDKDHTGEMLSNLLITSLSQARGLEVISRERLYDIQKEMGQADARTITP
                     SLASQIAKRAGVKTMLLGSILQKQPALAVTSRLIDVSSGKIVSSQRVTGFSTDQIFPL
                     VDSLAGQVRRDLAVTPAPAAEAKSVAEVTTNSPEAYRSYLEGVELQDKFYFVEAKAAL
                     KKATELDSNFAMAYFQLVTVEDNSELQRKALQKAWQLRNKAAEKERLQIQAAYVGGID
                     NNPAKAAEFLEELVRKYPHEQSAYIQLARTYVGLGLYEKSLQTQLTGLQTDSSDKNLW
                     NLLAYANAGLNRKAEALEAVNHYLKLAPGEPNPYDSKAEIYFVFGEVDSALYWYQKAI
                     SLRADFQSIEKLGHNALLRQDYATAEKCYQQFGSTSDKSQKAQAEDDLLLIPLHRGQL
                     QQVRKQVLNNLQTHLAQKLQNQIIGDYFALAGLSDKAGDYATMVEYAQKASAELKKNP
                     SDKIYGRDLLAWAYAKNGNAQMASKLLSELKKDVPEKSTAAQAQAAYTAALLAYEDGK
                     YDLAVTQFQKAFQPLLPNHAPQFHYAVCLLKSGHISEAIAELQRVTWWSPISLPIISL
                     FILPTSGYWPLASVKAHFWLGVAYEQQGEKEKAVKEYEKFLEIWKDADPDIKELSDAK
                     ERLAKLKTKA"
     gene            complement(7561..8547)
                     /locus_tag="AC16GM_00175"
     CDS             complement(7561..8547)
                     /locus_tag="AC16GM_00175"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR2922127.1"
                     /translation="MRIKNTPHDLADLVLEVLQNNRGGVTGEQLAEALRITPRLVAEL
                     VSGLKRNGYAIENKRSVYHLKEDVEPLTERTISQGLSTRHLGRHIFVFRSIGSTNDTG
                     FLLAESGAKEGTLLVTERQTKGRGRLGRSWHSPGKVGLWFSLILRPKVLPSQAPGLSL
                     AAALALAETIEALTSLEVEIKWPNDCLINGRKVAGILTELSAELDKINFVVVGVGVNV
                     NHKKNDFPLGLRRKATSLFIASDQKVSRVKLLQEFLVRFEAIYGQFVAEGLKPLLPRL
                     KKRTHLLGKQVKLQLGKKVIRGVAADLDPSGSLVLETKTGREVVTAGEVTVV"
     gene            complement(8544..9428)
                     /gene="nadC"
                     /locus_tag="AC16GM_00180"
     CDS             complement(8544..9428)
                     /gene="nadC"
                     /locus_tag="AC16GM_00180"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR2922128.1"
                     /translation="MDWEKIDRLIALAWEEDVGSGDITTSLFFPPDKTGRAVITAKSE
                     GVLAGAPVVERVFQYARSKTASSAALECQWQKRDGDTLAAGERVAEIAGRLATILAAE
                     RIALNFLGHLSGIATLTRKFVEAAAGTQAKILDTRKTTPGLRELEKYAVKIGGGVNHR
                     LGLYDQIFVKGNHIRAGGGLPRVLEKLKSTPAPKASNESLPNLPVVEVRSLAELDQAI
                     AGGAEWILLDNFSVADLQKAVSRVRSQPKKISLEASGGVTLANVRAIARTGVGFISVG
                     ALTHSAPASDFSLEILSE"
     gene            complement(9665..12385)
                     /gene="ppdK"
                     /locus_tag="AC16GM_00185"
     CDS             complement(9665..12385)
                     /gene="ppdK"
                     /locus_tag="AC16GM_00185"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR2922129.1"
                     /translation="MKKQAVVNKEVSKPNAGHSKKYIYFFGSGKADGTGEMKELLGGK
                     GAGLAEMTRLGIPVPPGFTITTELCNLYYQNNLKVPKGFDGEMRAYLARVEKTIGSRF
                     GHPENPLLVSVRSGSKFSMPGMLDTILNLGLNDQTVKGLAKKTKNERFAGDSYRRLIQ
                     MFGNVVLGIDKTEFEAIFQKKKEERQVKQDASLAVEDLKQIISAYKNLIKRKTGEEFP
                     ADPWTQLVQARDAVFKSWNNPRAITYRQLHGIPGHVGTAATIQAMVFGNLGEGSASGV
                     GFSRNPSTGENVLYGEYLANAQGEDVVAGSRTPKPIAQLAKEIPPAYKQLHEIARRLE
                     RHNRDTQDFEFTIQAGKTFMLQTRTAKRTTHAAVRIAVDMVKEKLISKEEALRRIEPE
                     QLNQLLHPRLDPEAEYRPLTKGLAASPGAAAGRVVFSAEEAVKRTRTGEAVILVRNET
                     NPDDIHGMAAAVGILTARGGMTSHAAVVARGMGKCAVVGAEMIRVFEEKRYFSVGSTV
                     VREKELVTLNGSKGEVIIGSVPTLDPEVTGEFAEFMRWADQTRTLGIRTNADTPADAA
                     VAVKFGAEGIGLCRTEHMFFATDRLPVVQEMIVAAAREDRVKALQKLLPFQKADFKGI
                     FEAMGGRPVTIRTLDPPLHEFLPRKEDLLAQIADLKAKQADPLLIDEKERILKRVEEL
                     AEFKPMQGHRGSRLVITYPEITEMQARAIFEAACELVRAGDEVKPEVMIPLVGEVREF
                     LNQKEVVDRVAAETMKKYKVKLKYLVGTMIEVPRACVMAEEIARAADFFSFGTNDLTQ
                     MTFGFSRDDAGKFLTGYLEKGILPSDPFVHLDQKGVGQLIDQTVKKGKEVRKDLKVGI
                     CGEHGGDPQSIAFCHTLKLDYVSCSPYRVPIARLAAAQAALGAVNGLGTD"
     gene            13085..16492
                     /locus_tag="AC16GM_00190"
     CDS             13085..16492
                     /locus_tag="AC16GM_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922130.1"
                     /translation="MAKKVFVIFLAVLFVAVGSVSWAKVTGKNAPITVTAMKKAAPTY
                     EVRTPTAGESEDLLQAAKKRTHRFIGASRYAPQGTLTVPSVGTDRLLGNTFSETQGNA
                     SDGAKQCTVDLTGTAHTSWRDTQSGGRPATNRGVYWTCVTAGGAVVNPGGTRLPSTGS
                     QNYRFSQNDFTPDGRSVLTGHRSLAACATPAGLSQWMAADGAACANSWALTVQGCMDT
                     SLNLIWPDITTTADSTIHVIGTDSRVNPGATPFCYARSTDGGATWSVPSLITGTGATG
                     NSAGPGRDTLAPGSYFPGTLGGGSKQGINLDMRLIGSRKSKQLALVYVVTGAPEQPGA
                     STQASGNIWIRRSTDAGLSWEEAKALTCFDSTSKITAQTNQYSAIPSAIYDGNDDLHI
                     IFAGDDYNTTTLTASFTGGTLYHMQVPSVIATGTDCPGGAAIATLHPIAPGNFPNDAN
                     LIGDLFCGSIVRPNLATPGILDGTKKGVLYAVWNQFSGADLASQNDTSSSDVGPLYNG
                     ELYAATSTDFGCTWSHVQNLTNTKTPGCGAGLTPNCASEQNAWVADIVKDSVNIIYTL
                     DFFAGQLSGGVTTYPPGNEVHLLRVDQAALAPTLEPRLSASPGAFREVDLHPRDSMVL
                     SVNVTNPGTATLNVTNVAVNGPDSPPLRTGVSTTSFSIATCVSTPTVVKCSLNAFNIT
                     YPVDALTPVVGYFPYSIDFTSNNTGGPVSVPIDLYVIDSFVRGTAQTFTSGTVAGNAA
                     SVALKTSTLVNSNGTIGENGTGMVYNQPPDTVYRIFHHGPVMMYGAGPTLVGYSYQAT
                     NTSALTDLAVDGDGAQTVVRSVFTGKGDSTIVGEQILQFPTGTANAEFIIATYRYVHP
                     ATSGYPPAPTAPTLAPVYLGGLGDWDIPNGTSSQGSLNLVGISLGDSLLFIYGTDPAD
                     SSTPLSDYGGYYLLDGGGGTIWARAGVRNAVYDGTNPPPDSIIRVVKDSGVNLALGDT
                     GVEIGKGRDHSIWYSVKFASMGQSDFDLYAKRKGADAGSWLPASDSTLRKNAKQVTFA
                     LVTSTAGQSGLETNAATAKAWALAHPALPAGGLLLGHLKGDLTLDEVVDIGDIVSLIQ
                     CVVFSSSCGTSFCIPDVTDDGVVDVGDIVTEINDVVFSTPDYGRCDF"
     gene            complement(16927..17409)
                     /locus_tag="AC16GM_00195"
     CDS             complement(16927..17409)
                     /locus_tag="AC16GM_00195"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR2922131.1"
                     /translation="MSNVARLNLAGYATLVTTVTAGRDPVFLDSGNCEILITTLYYGC
                     NQGWYGLLAFVVMPDHLHLICVPEKKNVSQSMHSLKSFSAAKINERRGTSGSLWQPSF
                     RNFVIPSIGILINKVRYVHENPVRQGLAATPDAYPWSSANPKYATVLSRYLGLGEPRS
                     "
BASE COUNT         3853 a         5345 c         4716 g         3817 t
ORIGIN      
        1 ttttgagtat caatacaacc gtcaggaaaa tcgctaccag cctgagtccg tttctgacgt
       61 tttgcgcgac acgctggcac tggaccttca acgccggcag aacatcctct ggaatcaaga
      121 ggtgcgcgca accgttggga aacaattgaa aaacgttgcc ggagtcaccc tgggctggca
      181 ctgggaaaaa ggcgcggaag attacggcgt gaatttcaaa gaccaaaggc gcgaaaccgg
      241 cgaactctct ggtactgtcg gtctgcgccc atcaagttcg gacaccgtcg cggtcttcgg
      301 ttccatcggc gtcacctcct acttcgtgcc gccgatctcg ggctttcttt ctgaccgcga
      361 tcttttgaac ggcaccgttt cgctttccgc cgggcaccgc ttcagtccct attttgacct
      421 gaaactgacc ggctcccgcc gcttgctcaa acagatttat ctgtcgggcc gctactccgc
      481 caacaaccgc actaccgaca actacctcct ggctcccgtg ctctcgtacc gcttcccagg
      541 cggctttgcc ctgcgccagg ggttcaactt gggagctaac tacgtcacct tcgattttct
      601 gaaaacctcg cccaatgccg accacacgat tttgcgtcag gccagttcgg ccagcgaaat
      661 gaccctgcct ttgaccgcac gacttgcctg gattctgggc tacacctacc gctacgaaga
      721 tttcggcggc ttgctctggc gggaccagtg ggtcgagcag accacctggg accggcggac
      781 gaacatcgtg gacgcccggt tcacctacca gtgggcgaaa tcgttgggcg tttccggcgg
      841 cttcatctgg gagcagaagt tcacctacga tcacgtcgcc agattgagca cgaataccat
      901 tgagcgcatt cgctcgctca gtttcattcg gaaaaccgtc actttcggct tgacctatgc
      961 actggatggc gccaactttc tgcgcgccga ttaccagcgc cgggcgcagt ccggttccgg
     1021 gttctcggtg ggcgacaccg actgggccac cgtttcttat gggagaacat tctgatatgc
     1081 ctggctctaa ctttctcaaa cgtccaactt ctgcgacagc cgtgattgtg tcgctggccc
     1141 ttctggtcgg cgcctgtaag aatcctttct ctacccgcga agccgaaaaa cccttaggac
     1201 agaccggtac ctggtctttc cccgcctcgc ccgacctggt gctgcggaat ctcctctttt
     1261 ccttcaacga acgcgtagtg gccaattaca ccacctgctt ttccagcgat ttcatctacg
     1321 ccgtcgattt ctttatttcc ggcgttccgg acacttcccg ttcctggggg aacgctgccg
     1381 aactcgaact cgcctccaaa ctcttcaatt tcgccagcgc ccatgtcgat tcgctcaatt
     1441 acgttctttc actggcgccc gatccgtctc gcaccgacat ccggcaggac acgctcgcca
     1501 tttttttccg cacctatcag attttgcagc tccgcctcaa accccaaccg cccgaaacga
     1561 cggtgtacgc aggcgtggcg accttccatc tgcggcagga tagttttaac ttctgggccc
     1621 tcgaccgctg ggacgaccag catcaatcga gcactatccc aagttggggc gacctgaaag
     1681 cgagtttccg gtgaggcggc ggctccttgc ggtaattttt cttctattgg ttattgcaac
     1741 ggcctgccgg aatcccttcg cgccgccgga ggccggcccg gcccgttccg gacctttggc
     1801 ccggcagacc gatcccgact cgactttggc gaacttccgc tacgcctacg agcgccgcga
     1861 cctcgacgtc tacgagaact gtcttgcctc caatttcgtc ttcctctata ttgaccagga
     1921 cctcaccggc cagatcgaac aggtggcggt gccgcgcgac ggcgcttccg gcgacctggc
     1981 ccgaaccgag cggctcttcc gggtcttcga tgccgtcagt ctggacacct ggaacgttat
     2041 tgccgagccg gacgattcga cggcgaccgg tgtggtcaaa cggcggcggg tgaacttcca
     2101 tctttcggtg caggatttga ccggcgactt caacttcgac gaataccagg cctccggctt
     2161 tgcccgcttc accttcgagc agtcgaaaac tgacaacctc tggcgcatcg tcacctggga
     2221 agatcagtcg tttacccaat aaaaaccgct atgcccaaac tctccgtaaa tgttgaccac
     2281 gtcgccaccc tgcgcgaagc gcgcaaaatc aaagagcccg atccggtctt tgccgcgatg
     2341 ctggccgaat tggccggcgc cgacggcatc accgtgcatc tgcgcgaaga tcggcggcac
     2401 atccgcgacc gcgacctcta cgtcttgaag gaagtggtca agaccgaact gaacttagaa
     2461 atggccgcca tcccgcaaat ggtggagcgg gctttggaag tgaagccgca tctggtgacg
     2521 ctcgtcccgg aaaaacgcga ggagttgacc accgagggcg ggctggacgt gaccggtttt
     2581 tccgagcgga ttggcgcagt tttgaaacaa atgaaaccaa ccggcattcg ggtgagtttg
     2641 ttcatcgatc cggaagtcga gcagatcaag gccgcggcca aactgcgcgc ggagattatc
     2701 gaactttcca ccgcccgcta cgccgagagc aaaaccgaca aagaaatcga tgaagaactg
     2761 aaaaaaatag accagatgac cgatatggcc gacaaattgg gcatcgaagt ccacgccggc
     2821 cacgggctga actaccggaa catcgtgccg cttttggaga ttgagaaaat caccgagtac
     2881 gccatcggcc acgccatcgt cgcgcgggcg gtcttggtgg ggttctccca agccgttaaa
     2941 gaaatgatac agattattag ggagagatgt ccaaattaac tgcttgatag tcagtaagat
     3001 acactaaatt taacagcatt tttcaatttg actcaaaatg ttaaaaaaag attttccttg
     3061 acaaaaaggg tgaaaaagcc gaaattaaac aatgtatata caagtttgga gcaggtcact
     3121 acatattggt ctatagggga cgagatttaa aaaccaaaat cgaaagtggg cggcaggatt
     3181 tgaacctgcg atggtccttt tcaaggggac tgccttgacc ggacttggcc acgcccactc
     3241 tttcgaagat agccggtggt gggattcgaa cccacgtagc tctgaggctt cggcttgcta
     3301 agccgattgg gtttatccac ttccctacac cggctattag tttctacaag ccccacaaga
     3361 cctgagacca atagtcagtt ctaactaatt acttgctatt tctaccctta ttaaccttga
     3421 tcctccatcc attaccttcc ttaggaatcc ctccatattt cccgtttgca tatctgtatc
     3481 cgagttcgtg caatcgaatt tccatcgcca tttgtgaaac accgaaatgt tttttcaatc
     3541 tttcaccgta aatgtcaagg tcaacgaccc ggttctcatc gactactcta aaaacctcct
     3601 ttgaaggcat catcatcgct gctgcatatc tgtttgcttg atattcgata gggtcatact
     3661 gttttccatt tacctgatta gtccgacaaa atgaaggagc aaggtccttt ttcttttctc
     3721 cgaatagctc tttctgattc ttcgttggcc aaacatgtaa agcgtaatgt gctccttcat
     3781 gaaaagctgt aaatctttgc atttgttcag atacggtaca attcaccaga atcagtttgt
     3841 catcatcgtg gaaaaattgg ccttccgggt aaagacaacc aagcaattct tcaccttcag
     3901 ccgctgccag gccatgctga tcaaaatcga tgtactccgt cgtcaacccg aagagggtat
     3961 ggaagacatc ctctggatcg agaggaaagc tcaaagggaa gttacccttt tggtccttat
     4021 agcgagcgaa gtccccaagg gctattcgtt caatgttctc ttggggaatg aattggtgcc
     4081 tcattcattt tcctttttct ttaaatttct tcctcaaata ctccggcgct ttaacagggt
     4141 tttgtgccaa aatttcacta atgtcttcag ggatatagcc agccagtagt tttaattctt
     4201 cctcgctata tttgaaatac tcagcaatcc tcctaataag ttcagctgat ggttttttgt
     4261 atcctaattc caggttactt aaatgggtat aatcaattcc cagttccggg gctaacgcct
     4321 tgatacctag ccccttagcc tctcttaact gccgcaattt cctgccaaaa agtttgtgca
     4381 tactcggtct tcctcggaca acctaaatcg cccttcctaa tttgtcaagt ctttttttgg
     4441 gggggatttt ttagttatag cacactcacc tccccggctg tcaccacttc tcggcgggtt
     4501 ttgctttcta agacgtgagg taattgctaa ccaaatcacc aagtgatata ctcgcctcca
     4561 tgccagaact ctcagaccgc tatctgctta agagagccaa agaatgatcg gcaaaacgat
     4621 ctcgcactat aaaatcatag agaaactcgg cgagggcggc atgggcgtgg tctacaaagc
     4681 ccacgacctc aaattggatc gcctcgtggc gttaaaattc ttgcccaaac atttgacttc
     4741 cgacgccacg gaaaaggaac gcttcatcca cgaagcccgg gcggcgtcgg ccttgaacca
     4801 ccccaacatc accacgatcc acgagattga tgaatttgag gggcagatct ttatcgtcat
     4861 ggagttctgc gaagggaaaa ccttacggca aatcgttgaa tcggaaacgc tccccattaa
     4921 gaaagtctta gaggtcgcca ctcaagtctg cgaaggtctg gctttagcgc acgagaagtg
     4981 gattgtgcac cgggacataa aatcggacaa catcatgctc accgcccggg gtcaggtgaa
     5041 gattatggac ttcggcctgg ccaaattgcg ggggaggggg gaggcccaaa ccggctccac
     5101 gctggggacg gcggcctatc tatcaccgga gcaggcgcgg ggggaggagg ttgaccagcg
     5161 cagcgacatt ttctcattcg gggtggtgct gtatgagctg ttggcgaaac ggctgccgtt
     5221 tcagggcgag caccaggcgg ccttgaccta cgccatcatc tacgaagaac cccagccgct
     5281 cacccgcttc aataatcagg tctcggcgaa gttggaagac atggtcttca aggccttagc
     5341 caaagaccgg gaggagcgct accagcacat tgacgacctc ctggccgact tgcggcgtga
     5401 gaagaaaaac gtcgaatacg tgaaggttcc ccatgttcct tcagcacccg ccccgccagc
     5461 gccatcaaag aagaacctgt ggaaatatct cgtccccact tccgcggtcg tggtgctggc
     5521 cttagtcatt ttcatcctca aacccttcaa atttgaagtc gggccggagc agaaggcggt
     5581 ggcggcggaa aattctctgg cggtgatgta cttcgaaaac atccccgatc cggaggataa
     5641 agaccatacc ggagagatgc tctcgaatct tctcatcacc tccctctcgc aggcgcgggg
     5701 gctggaagtg atcagccggg aacggctgta cgacattcaa aaagagatgg gccaggccga
     5761 cgccagaacc ataaccccct ccctcgcatc gcagatcgcc aaacgcgccg gggtcaagac
     5821 gatgctcttg gggagcattc tgcaaaagca gcccgcgttg gccgtaacct cccgcttgat
     5881 tgacgtttca tcggggaaaa tcgtgagttc ccagcgcgtc actggctttt ccaccgacca
     5941 gatattcccg ctggtggata gtctggccgg tcaggtcaga cgcgatctgg ccgtcacccc
     6001 agcaccggcc gccgaagcca aatccgtggc ggaagtgact acgaattccc ccgaagccta
     6061 ccgctcctat ctggaaggag ttgaacttca ggacaaattt tacttcgtcg aggccaaagc
     6121 cgcgctcaag aaagccaccg agttggacag caactttgcg atggcctact tccagttagt
     6181 aactgtcgaa gataactctg agttgcagcg aaaagcgttg cagaaggcct ggcagctgcg
     6241 caataaggcc gccgaaaaag aacgcctgca gatccaggcc gcgtacgtcg gcgggatcga
     6301 caacaatccc gccaaagcgg ctgaattctt agaagaactg gtccgcaaat atccgcacga
     6361 acagagcgcc tatatacagt tagcgcggac gtacgtaggc ctgggactgt acgaaaaatc
     6421 cctgcaaacc caattgaccg gactgcaaac tgattcctcg gataaaaatc tctggaattt
     6481 attggcctat gcgaatgccg gcctgaaccg taaagcggaa gcgctggaag cggtaaacca
     6541 ctatctgaaa ttggcgcccg gagagcccaa cccatacgat agcaaggcgg aaatctactt
     6601 tgtcttcggg gaagtagact ccgctctgta ctggtaccaa aaagcaattt ccctgcgcgc
     6661 cgatttccag agcattgaaa aattgggtca taatgcgctc ctgcggcagg actatgcgac
     6721 cgccgaaaaa tgttatcagc agttcggctc cacgtcggat aaatcgcaaa aagcacaggc
     6781 ggaggatgac ctgctcctga ttcccctcca tcgcggacag ctgcaacagg ttcggaagca
     6841 ggtgctgaac aacctgcaaa cccatctggc tcaaaaacta caaaaccaga ttatcggcga
     6901 ttatttcgcg ctggccggcc tctccgataa agccggcgat tatgcgacta tggttgaata
     6961 cgcgcaaaaa gcatcagcgg aactcaaaaa gaatcccagt gacaaaatct acgggcgcga
     7021 tctcttggcc tgggcgtatg ccaaaaacgg caatgcccaa atggcctcga agctgctgag
     7081 tgaactaaaa aaagacgtcc ctgaaaaaag caccgcagcg caggctcaag cagcctatac
     7141 cgccgcgctt ctggcgtatg aggatgggaa atacgacctc gccgtaaccc aatttcaaaa
     7201 ggcgttccag ccccttttgc caaaccacgc tccgcagttc cactacgcag tttgcctgtt
     7261 aaaatcaggt cacatttcag aagccatcgc ggaactgcaa cgggtgacct ggtggtcgcc
     7321 gatttcctta ccgatcataa gtcttttcat tctgcctacg tccggctatt ggccgcttgc
     7381 ctcggtgaaa gcccactttt ggctgggcgt ggcgtatgaa cagcagggag agaaggagaa
     7441 agcggtcaaa gagtacgaaa agtttttgga gatttggaaa gacgccgacc cggatattaa
     7501 agaattgagc gatgcgaaag agagattggc aaaactaaaa acaaaagcat agacccgcgc
     7561 ttacaccaca gtcacctccc cggccgtcac cacttcgcgg ccggttttgg tttccaagac
     7621 cagagagccg gagggatcga ggtccgccgc cactccgcga atgacctttt tgcccaattg
     7681 aagtttcacc tgcttaccca aaaggtgcgt gcgtttcttt agccgcggca aaagcggctt
     7741 taagccctcc gccacgaact gcccgtaaat cgcctcaaag cgcactaaaa actcctgcaa
     7801 gagtttgacc cgggagactt tctggtcact tgcaatgaaa agcgaagtcg cctttctccg
     7861 gagccccaga gggaaatcgt ttttcttgtg gttcacgttc acccccacgc ccaccaccac
     7921 aaagttgatc ttatccaact ccgccgagag ttcggtcaaa atccccgcca ccttgcgccc
     7981 gttaatcagg cagtcattcg gccatttgat ttcaacttca agcgaggtta gcgcttcaat
     8041 cgtctccgcc aaagccagag ccgccgcgag cgaaagcccc ggcgcttgag aaggtaagac
     8101 ttttggtcgc agaatgagcg aaaaccataa acccaccttc cccggcgaat gccaggagcg
     8161 tcccaaccgc ccccggcctt tggtctgccg ctcggttacc agcagggttc cttctttggc
     8221 acccgattcc gctaaaagaa acccggtatc attggtagaa ccgatagagc ggaagacgaa
     8281 gatgtggcgg cccaaatgtc gagtcgaaag gccctgagag atggtgcgct ccgtcaaggg
     8341 ctcgacatct tctttcaggt gataaacgct ccgcttgttt tcgatagcgt aaccgttccg
     8401 cttcaacccg ctcactaact ctgccaccag ccggggggtg atgcgcagcg cttcggctaa
     8461 ctgttcgccg gtgacgcccc cgcgattgtt ctggagaact tccaagacca gatcagccag
     8521 gtcgtgaggc gtatttttga ttctcattcg gagagtattt ccaggctaaa atcagacgcc
     8581 ggtgccgaat gcgtgagtgc ccccaccgaa ataaaaccga ctcccgttcg ggcaatggcg
     8641 cggacgttag ccagcgtcac cccgcccgac gcttcgaggg aaatcttttt cggctgcgag
     8701 cgcacccggc tcaccgcctt ctgcaaatcg gcgaccgaaa aattatccag caaaatccac
     8761 tccgccccgc ccgcgatagc ttgatcgagt tccgccagcg agcgcacctc cactaccggc
     8821 aggttgggca gcgattcgtt ggacgctttt ggagcgggag ttgatttcag tttttccagc
     8881 accctcggaa gcccgccccc agcgcggatg tggtttccct tcacgaaaat ctggtcgtac
     8941 aatcccaagc ggtggttcac ccccccgccg attttgaccg cgtacttctc caattcccgc
     9001 aatccgggcg tcgtcttccg cgtgtctaaa atcttcgcct gcgttccggc tgccgcttcg
     9061 acgaactttc gcgtcagcgt ggcaatgccc gaaaggtgtc ctaagaagtt gagcgcaatc
     9121 cgctcggccg ccagaatcgt tgccaatcga ccagcaatct cagcaactcg ttcaccggca
     9181 gccaacgtat cgccatccct tttttgccac tggcattcga gcgcagcaga agaagcggtt
     9241 tttgacctgg cgtactggaa aacgcgctcc accaccggtg ctccggccag aacgccctca
     9301 gatttggcgg tgatgaccgc ccggcctgtt ttatccggcg gaaagaatag agaagtcgta
     9361 atgtcgccgg acccgacatc ctcctcccac gccagcgcaa ttagacggtc aatcttttcc
     9421 cagtccattt gcacgggaaa aatataagtc aggcaagatg cctgacctat ggagaaagat
     9481 gaaaagacac ccgcaaaaaa tcaaataaaa acccctcgcc ccggtttcct gaccgcgccc
     9541 gcagatcccc ttcaccctcc ataattcgtt cttccccctc catggccgag gcatctcgat
     9601 agagtcgtat cgacctgcct cgggttcgtt cgtcaggctt ccagcttcgg cgcactaatc
     9661 cgttttaatc cgttcccaat ccgttgacag cccccagggc cgcctgcgcc gctgccagcc
     9721 gggcgatggg cacgcggtac ggcgagcagg agacgtagtc gagtttaagc gtgtggcaga
     9781 aggcgatcga ctgggggtct ccaccgtgtt caccgcaaat gccgactttc aagtctttgc
     9841 ggacttcttt gcccttcttc acggtttgat cgatcaactg tcccacgccc ttttgatcga
     9901 gatggacgaa cggatcggag ggcaaaattc ctttctcgag gtacccggtg agaaattttc
     9961 cggcgtcgtc gcgcgagaaa ccgaaggtca tctgagtcag gtcgttggtg ccgaaggaga
    10021 agaaatcggc ggcgcgggcg atctcctccg ccatcacgca ggcgcgcggc acttcgatca
    10081 tcgttccgac cagatatttt aatttgactt tgtacttctt catcgtctcg gcggccaccc
    10141 ggtcaaccac ttctttctgg ttcagaaact ctcggacctc gcccaccagg ggaatcatca
    10201 cctcgggttt gacttcgtcg cccgcccgga ccagttcgca ggccgcttcg aagatggccc
    10261 gcgcctgcat ttcggtgatt tccggatagg tgattaccag ccggctgccg cggtgcccct
    10321 gcatcggttt gaactcggcc aattcctcca cccgcttcag aattctctct ttttcgtcaa
    10381 tgagcagcgg atcggcctgt ttggctttca ggtcggcgat ctgggccaag aggtcttctt
    10441 tccgggggag gaactcgtgc agcggcggat cgagagtgcg gatggtgacc ggccgcccgc
    10501 ccatcgcttc gaagatccct ttgaagtcgg ccttctggaa aggcaaaagt ttttgcaggg
    10561 ccttgacccg atcttcccgg gccgctgcca cgatcatctc ctgcaccacc ggcagccggt
    10621 cggtggcaaa gaacatatgc tcggtccggc agagcccgat cccctcggcg ccgaacttga
    10681 ccgccaccgc tgcatcggcc ggcgtatcgg cattggtgcg gatgcccaaa gtccgggtct
    10741 gatcggccca gcgcatgaac tcggcgaatt cgcccgtcac ttccggatcc agagtcggaa
    10801 ccgacccgat gatcacctcg cctttggaac cgttgagggt gaccagttcc ttttcccgca
    10861 ccacggtgct gcccacggag aagtaccgtt tttcttcaaa gacccggatc atttcggcgc
    10921 cgaccaccgc gcacttcccc atcccccggg ccaccaccgc cgcgtggctc gtcatcccgc
    10981 cccgggcggt gagaatgccg accgccgccg ccatcccgtg gatgtcgtcc gggttggttt
    11041 cgttgcggac caaaatcacc gcttcgccgg ttcgggtccg cttcaccgcc tcctcggccg
    11101 aaaagaccac ccggccggcc gcggcaccgg gcgaggccgc cagtcctttg gtgaggggcc
    11161 ggtactccgc ctccggatca agccgcgggt gcaaaagctg gttcagctgc tccggctcaa
    11221 tccgccgcag cgcctcttct ttagaaatga gtttctcttt gaccatatcg actgcgatcc
    11281 ggacggcggc gtgggtggtg cgcttggccg tgcgggtctg gagcatgaag gtcttcccgg
    11341 cctggatggt gaactcaaaa tcctgggtgt cccggttatg ccgttccagc cggcgggcga
    11401 tctcgtggag ctgcttgtag gccgggggaa tttctttggc cagttgagca atcggtttcg
    11461 gcgtccggct gccggccacc acgtcttcgc cctgggcgtt ggccaggtat tcaccgtaaa
    11521 ggacgttttc cccggtggag ggattccggc tgaagccgac gcccgaagcc gaaccttccc
    11581 ccaggttccc gaacaccatt gcctgaatcg tggcggcggt gcccacgtgg ccggggatgc
    11641 cgtgcagctg ccggtaggtg atcgcccggg ggttgttcca cgatttgaag accgcatctc
    11701 tggcctgcac cagctgcgtc cagggatcgg ccgggaactc ctccccggtt ttccgcttga
    11761 tcaaattctt gtacgccgaa atgatctgct tgagatcctc caccgccagg gaggcgtcct
    11821 gcttcacctg cctctcctct tttttcttct ggaagatcgc ttcgaactcg gttttgtcga
    11881 tccccaagac cacgttgccg aacatctgga tcaaccggcg gtacgagtcg ccggcaaagc
    11941 gttcgttttt ggtcttcttg gctaggcctt tgaccgtctg gtcgttcaag cccaagttga
    12001 gaatcgtatc cagcatcccc ggcatggaga attttgagcc ggagcgcacc gaaactaaaa
    12061 gcggattttc cggatgcccg aagcgggaac cgatggtctt ttccacccgg gccaaatagg
    12121 cacgcatttc accgtcgaac cctttgggaa ctttcaggtt gttctgataa tagagattgc
    12181 acagctcggt ggtgatggta aatcctggcg ggaccggaat ccctaaccgg gtcatttcgg
    12241 ccaaaccggc gcctttacca cccaaaagct ccttcatctc tccggtgcca tccgcttttc
    12301 cactgccaaa aaaataaatg tattttttgg aatgaccggc gtttggtttg ctaacttcct
    12361 tattgacaac ggcttgcttc ttcatttctt ttcttttctt gatcttgacc ttttccgaca
    12421 ttatccctcc tgaaagtttt accagcattg cgaaacggcg caacactcaa tataccggat
    12481 gccggtcgat tatcaagccg gaaatcactg gcaaagctcc cgaatttttt ttatagaaaa
    12541 cgccaaaaaa tcacttgaca aaaaaatggg gggagggtat gttatccctt gaagttgagg
    12601 gccctttcgg ggtcgggtgg ttggtcgcca acccaagccg aaaggaacaa cttcgcaaag
    12661 gctcgaaagt aagtttagtc gcggagatac gcggcgagag caaatcactg cggatctcaa
    12721 cccagtcctc ttttcggcgc tctcccgcca caggcgggag ggagcggctc acagacgctg
    12781 gccgaggtag ccaagttcca catcggcaaa cgacgcgggg tgagattttt gtctttgatg
    12841 gcgagtccgc ctgaggcgga cgaaccatct ttcgggcatc aaaaatcgat ccgcgtcaag
    12901 aaggaggtgg tagttctcta agcgaggaaa tcggtatact tcggtcttgt tcggctctcc
    12961 gggcggtcct cccgtacccg catgccgcag ggaaaggaga caggaaaaga gagaaagtcc
    13021 cgcctatggg cgggacgaga caagatggag gcaacgtttc acaatcgtta aggagttaaa
    13081 ctacatggca aaaaaagtat tcgtcatctt tctggcggtc ctgttcgtgg ccgtcgggag
    13141 cgtttcgtgg gccaaggtga cgggcaagaa tgcgccgatc accgtgacgg ccatgaaaaa
    13201 ggccgcaccg acctacgaag tgcgtacccc gaccgccggc gagtcggagg atctcctgca
    13261 ggccgccaag aagcggactc accggttcat tggcgcttcg cgctacgccc cccagggcac
    13321 cctcacggtg ccgtcggtgg ggaccgaccg cttgctgggc aacaccttca gtgaaaccca
    13381 ggggaacgcc agcgacgggg ccaaacaatg caccgtcgat ctcacgggga cggctcatac
    13441 ctcctggcgt gacacccagt ctggcggccg gccggcaacg aaccgcgggg tctattggac
    13501 ctgcgttact gccggcggtg cggtggttaa cccgggcggc acccgtcttc cctcgacggg
    13561 cagccagaac taccgcttct cgcagaacga cttcacgccg gatggccggt cggtacttac
    13621 cggtcaccgc tcgcttgcag cctgcgctac tccggcgggg cttagtcagt ggatggcggc
    13681 cgacggggct gcctgcgcga actcctgggc actcaccgtg cagggctgca tggacacttc
    13741 tctgaacctg atctggccgg acatcaccac cacggccgac agcacaatcc acgtcatcgg
    13801 gaccgacagc cgggtcaacc cgggcgcgac gcctttctgc tacgcgcgct cgactgacgg
    13861 cggggccacc tggagcgtgc cgtcgcttat taccggcacc ggcgctaccg gcaactcggc
    13921 cggcccgggc cgcgacactc tggctcccgg atcgtacttt ccgggaactt taggcggcgg
    13981 cagcaagcaa ggcattaacc tggacatgcg gctcatcggc tcgcgcaagt ccaaacagct
    14041 ggctttagtc tacgtcgtta ccggcgctcc ggaacagccc ggcgccagta cccaggcctc
    14101 cggcaacatc tggatccgcc ggtcaaccga tgccgggttg tcctgggaag aggccaaagc
    14161 cctcacctgt ttcgactcga cgtccaagat taccgctcag accaaccagt actccgccat
    14221 cccttcggcg atctacgacg ggaacgacga cctgcacatc atcttcgcgg gcgatgacta
    14281 caacaccacc accctgacgg cctccttcac cggcgggacc ctgtaccata tgcaggtgcc
    14341 ctcggtgatt gccacgggaa ccgactgtcc gggcggagcc gccattgcga cgctccaccc
    14401 gatcgcgccg gggaacttcc ccaacgatgc caacctgatc ggggacctct tctgcggcag
    14461 catcgtgcgg cccaacctcg ccacgccggg aattctggat ggaaccaaga agggcgtcct
    14521 ctacgcggtc tggaaccagt tctccggggc ggatctcgcc agtcagaacg acacctcgtc
    14581 ttcggacgtc gggccgctct acaacggcga gctctacgcg gccacctcca ccgacttcgg
    14641 ctgcacctgg agccacgtcc agaacctgac caacaccaag acgccgggct gtggcgccgg
    14701 tcttacgccc aactgcgcca gtgagcagaa cgcatgggtg gcggacatcg tcaaggactc
    14761 ggtcaacatt atctacacgc tcgacttctt cgccggtcag ctctccggtg gggtcacgac
    14821 atatccgccg gggaacgaag tgcacctgtt gcgggtggac caggcggccc tggccccgac
    14881 cctcgagccg cggctctcgg cgtcgccggg cgccttccgc gaagttgacc tccatccgcg
    14941 cgatagcatg gtgctgagcg ttaacgtgac caacccgggg accgccacgc tcaacgtaac
    15001 caacgtggcc gtcaacggtc ccgactcgcc gcctttgcgc acgggagtct caaccaccag
    15061 cttcagcatt gccacctgcg tctctactcc gacggtggtg aagtgctccc tgaacgcctt
    15121 caacatcacc tacccggtcg atgcgctgac gccggtcgtg gggtacttcc cgtactccat
    15181 cgacttcacg agcaacaaca ccggtgggcc ggtctccgtg ccgatagacc tctacgtcat
    15241 cgactccttc gtacggggga ccgcacagac cttcacatcc ggaacagttg ccggcaacgc
    15301 cgcctctgtg gctctaaaaa cttctacttt ggtcaactcc aacgggacga tcggagagaa
    15361 cggcaccggc atggtttaca atcaaccccc cgacacggta taccggatat tccaccacgg
    15421 gccggtgatg atgtacgggg cgggtcccac gctggtgggc tacagctatc aggccaccaa
    15481 cacgtcggcc ttgaccgacc tggcggttga cggtgatggc gctcagacgg tcgtccgttc
    15541 ggtctttacc ggtaaaggtg actccactat cgtcggggag cagatcctgc agttcccgac
    15601 gggcaccgcc aatgccgaat tcatcatcgc gacctaccgc tacgtccacc cggccacttc
    15661 gggttatccc ccggctccca cggcaccgac gcttgcgccg gtttacctgg ggggtttggg
    15721 tgactgggac atcccgaacg gcacatccag tcagggctcg ctcaacctgg tggggatctc
    15781 tttgggcgac tcgctgctct tcatctacgg caccgatccc gccgactcct cgactccttt
    15841 gagcgactac ggcggctact acttgctgga tggcggcggc ggaaccatct gggcgcgcgc
    15901 gggcgtgcgg aacgcagtct acgacgggac caacccgcca cccgactcga tcatccgcgt
    15961 ggtcaaagac tcaggcgtga acctggccct gggggacacc ggtgtcgaga tcggcaaagg
    16021 gcgtgaccac tcgatctggt actcggtgaa gttcgcctcg atgggtcaga gtgactttga
    16081 cctctacgcg aagcggaaag gcgccgatgc cggttcctgg ctccccgcaa gcgatagcac
    16141 gttgcgcaaa aacgccaagc aggtaacctt cgcgctggtg acttccacgg ccggtcagtc
    16201 gggcctggag accaacgcgg cgacagccaa agcctgggct ctcgctcacc cggctctgcc
    16261 ggcgggtggt ctgctcttgg gccatctgaa aggtgacctg accttagacg aggttgtcga
    16321 catcggggac atcgtgtcgc tgatccagtg cgtggtgttt tcctcttcgt gcggaacgtc
    16381 cttctgcatt ccggacgtca ccgacgacgg cgtcgtcgac gtgggagaca tcgttaccga
    16441 gattaacgat gtggtgttct ccacgcctga ctacggacgg tgtgacttct aatatctgag
    16501 gtcacaggtc aaaaaaagtg gtgggtcgcc agacccaccg ctttttattt gccgcgaaga
    16561 tcgcggtcag gcaagatgcc tgacctatgg gggaatgaat tggacggaat gcctcaaacc
    16621 aaacttccat agccgaggcg tcccgcctcg gattctttcg gtcaggttag gaagcctgac
    16681 caatggtggt ggagagacga aatccagaat caagcctcca tagccggggc ttccagcctc
    16741 ggatttcttt cggtcaggca aggatgcccc gccagaggcg ggactgaaag cctgacctat
    16801 ggatgggagg gagacggagg gtcgccaaga gatgggccaa ttgaaacttc tatagccgag
    16861 gcctcccggc ctcggattca catgggggtc aggctggaag cctgacctat ggaacggcgt
    16921 tttatgtcaa ctccgcggtt cacctaaccc cagatacctt gagagaactg tggcatactt
    16981 cggattcgcc gaagaccacg ggtaggcatc gggagtcgct gccaaaccct ggcgcaccgg
    17041 attctcgtgg acgtagcgta ctttgtttat gagtatccca atacttggaa tgacaaaatt
    17101 gcgaaaagaa ggctgccaga ggcttccgga cgtaccccga cgttcattga ttttcgctgc
    17161 cgaaaaactt ttgaggctgt gcatgctttg agaaacgttc ttcttctccg gaacacagat
    17221 taaatgcaga tggtccggca tcacgacgaa cgccaagaga ccataccacc cctggttgca
    17281 cccatagtag agcgttgtga tcagaatttc acaattcccc gaatcgagaa acactggatc
    17341 gcggcccgcc gttaccgtgg taaccagagt ggcatagcca gccagattca aacgcgctac
    17401 gttcgacaca agcaacgttc cttccatagc cggggcttcc agccccggat ttccttcggt
    17461 caggctggaa gcctgaccta tggattaagg ataatgggcg ggaacgaaag gagaaatgaa
    17521 aatctcagag ccgagagcat cgtgccatcc gcccatctcc ctggccgaga cagcttgact
    17581 cggatctaat ccttcccttt ccatagccga ggcctcccgg cctcggattc ctttcggtca
    17641 ggctgggaag cctgacctat ggggattaag ggctttcctt catctctaag cggggatgtc
    17701 ctgtctcccg tctttcatcc atagccgagg c
//
LOCUS       DBNGZD010000008         5602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_139731, whole genome shotgun sequence.
ACCESSION   DBNGZD010000008 DBNGZD010000000
VERSION     DBNGZD010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_139731"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="AC16GM_00200"
     CDS             complement(<1..778)
                     /locus_tag="AC16GM_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HIR2922132.1"
                     /translation="MTSPIAAVLPRPKEAHATSAVRQKLEFKSGNEAAALAARDIGFH
                     LMGYYPITPSTEVAENLSKMLAEGEHSIAMIAGDGEHGAAGICYGAALGGGRVLNVTS
                     SQGLLYALEQLPVQSGTRVPMVLNLACRTVSGPLDIRGDHSDLYFTLNTGWIVLTARD
                     PQAVYDLNFAAVRIGEHQLVRLPVLVAYDGFFTSHQKRRIQVFDDAAAVQRFLGLPPA
                     YPTPLDPEHPATFGPYMNDPDLINNKMQLSLAMDAAHDVIR"
     gene            complement(789..1793)
                     /locus_tag="AC16GM_00205"
     CDS             complement(789..1793)
                     /locus_tag="AC16GM_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR2922133.1"
                     /translation="MDPKVADRSVLPAGYFEVRFESIGGLGAHAAGQVLAGAAVLRLG
                     MNGAAFSSYGSEKKGSPVRSFVRLGPVNRPIRTSAPVESPDAIVVFHAALLASPATIS
                     GLKAMGTLIINAPPGPLPEPLAALPRTARVVRVGALSIAVEEKSRPNAVLLGTLCASF
                     PFLNADEVKDALSQEFAGRHPEAVASNERAFVRGATEFETIENVGRAEGDLPPARFVP
                     VWGYETAPIGGIIPAAGNTAWNDLSVSRTGWLPVLEKGACIHCGLCDLVCPDLCLVWG
                     DGEPDGEYERKLMGIDYRYCKGCLRCVESCPTGALSKETETPGLAARLRVPLHPDLIG
                     "
     gene            complement(1939..5094)
                     /locus_tag="AC16GM_00210"
     CDS             complement(1939..5094)
                     /locus_tag="AC16GM_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2922134.1"
                     /translation="MLGVGLFFFFAARLSAQEPFFGKNKVHYRNFDWSYLQTDHFDVY
                     YYQDGYDLASWAANTLESAYVEIRSELNYDLRRRVPVILYQSHNDFQQTNVTPELIEE
                     GVGGFTESFKNRVVLPFMGSYEEFRHVLHHELTHALVFDMLYQNVLGSLLSRQYLFRL
                     PLWFAEGYAEYSSRHGWDPQADMVLRDATIHNYLAPLEYAGGFLAYKEGQSALLFLAD
                     RYGQDKIAEVLNKGRAALTMDKALKQSIGLSEQEFNDAWQKALKKEYWPEIARRQEPK
                     DFAKALTNHEKDGSHFNEKPAFAPTGDRIAVFSDKSGYTEVYIISAVDGKVIDRVVKG
                     ERSGDFESLHSYVSGIAWSPDATRIALVSKSKGEDALMVVRVKNHKVEKRLKFKMDGM
                     LSPAWSPDGSKIAFVGMRNGKADLYVYTLETKSLRRLTDDTDDDQDPAWRPDGKALAF
                     ASDRPTAGNGTADAQPYGNYNLFLYDLASDEITPLTKDGKNNRGPSWSPDGKKLVFSN
                     NRSGIDNLYILDLEQPASPPVASVKADPSPSANGGRDYPITNVLTGCFSPSWSKEGNQ
                     IALSSFYKGGFDIFVISDIKPATQNHEALSLTGYALRTQAGEKRFFASREEPTPPADS
                     ARDTAAAKAPRMAAKGGEGPSYVFRAGNVSRVSEDSTLAPDTAYVPPLAKINLEESKL
                     PSGAYRPHKYKVKFSPDLISGSVGYSTFFGLRGQSFLLVSDLFSNHQFFVATDLFNTI
                     DQSNVEVLYFYSPKRIDYGVGALHTRNFYIDPLDRLFADRVYGFLGSVAFPFTKFTRL
                     ELSFSGLNIDRRYFDPPFDDKSAQVALVGLSWINDTALWGLTGPANGQRWQVRVEQAP
                     KLANKGISYVAVEADYRKYWHFLKRYTFTLRLAGGASSSTSKRFFLGGTANWIGSRIA
                     NANVDRVYSINDLYLASVVTPLRGYDFYQFDGTHYALTNWELRYPFLDFLALRFPLPL
                     TISQVQGVAFYDMGAAWYENSTFRGTQGGGLRFNDIKASFGLGARANLGFLILRFDTA
                     WPTTIDHTGKPRYFFSLGADF"
     gene            complement(5262..>5602)
                     /locus_tag="AC16GM_00215"
     CDS             complement(5262..>5602)
                     /locus_tag="AC16GM_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2922135.1"
                     /translation="PASVGFIALFGVAVLNGVVLISYINQLRQSGMPLEEAIRTGCAR
                     RLRPVSMTALVTMLALTPLIFATGVGSEVQKPLAVVVVGGLLTSTTLTLLVLPVLYGW
                     FAGGKKEVRG"
BASE COUNT         1102 a         1672 c         1792 g         1036 t
ORIGIN      
        1 cgcggatcac gtcgtgcgcc gcgtccatcg cgagcgagag ctgcatcttg ttgttgatca
       61 agtcggggtc gttcatgtac gggccgaacg tcgccgggtg ctccgggtca agcggcgtgg
      121 ggtacgccgg cggaagaccg aggaaccgct gcaccgcggc cgcgtcgtcg aagacctgga
      181 tgcggcgctt ctggtggctc gtgaagaagc cgtcgtacgc cacgagcacc ggcagccgca
      241 ccaactggtg ctcgccgatc ctcacggccg cgaaattcag gtcgtagacc gcctgcgggt
      301 cgcgcgcggt gagcacgatc cagcccgtgt tcagcgtgaa gtagagatcc gagtggtcgc
      361 cgcggatgtc gagcggtccg gagacggtgc ggcacgcaag gttcagcacc atcgggacgc
      421 gcgttcccga ttgcaccggc agctgctcca gggcgtagag gagcccctgc gaactggtga
      481 cgttcagcac acggccgccg ccgagcgccg cgccgtaaca gatgccggcg gcgccgtgct
      541 cgccgtcgcc cgcgatcatc gcgatggagt gctcgccttc cgccagcatc ttcgagaggt
      601 tctcggcgac ctcggtggag ggcgtgatgg ggtagtagcc catcaggtgg aaaccgatat
      661 cgcgcgccgc taaagccgcg gcttcgttgc cgctcttgaa ctccagcttc tgccgcaccg
      721 cggacgttgc gtgggcttcc ttcgggcggg ggagaacggc agcgatgggg ctcgtcatag
      781 ggtccgcctc acccaatcaa gtcgggatgc aggggcacgc ggagccgggc ggcgaggccc
      841 ggcgtctccg tctctttgct caacgcgccg gtcgggcagg actcgacgca acgcagacag
      901 cccttgcagt agcggtaatc gatgcccata agcttgcgct cgtactcgcc gtcgggctcg
      961 ccgtcgcccc acaccaagca gaggtcgggg cagacgaggt cgcacaggcc gcagtggatg
     1021 cacgctccct tttcgaggac cggcagccac cccgtgcggg agaccgacag gtcgttccac
     1081 gcggtgttgc ccgcggcggg gatgattccg ccgatcggtg ccgtctcgta gccccacacg
     1141 gggacgaagc gcgcgggtgg aaggtcgccc tccgcgcggc cgacgttctc gatcgtctcg
     1201 aactccgtgg cgccgcgcac aaacgcgcgc tcgttcgatg cgaccgcctc cgggtgcctg
     1261 cccgcgaact cttgggacag cgcgtccttc acttcgtcgg cgttcaaaaa cgggaaggag
     1321 gcgcacagcg ttccgagaag taccgcgttc gggcgggact tctcctcgac cgcgatggag
     1381 agcgcaccca cccgcacgac acgcgcggtg cgaggcagcg cggccaacgg ctccgggagg
     1441 ggtcccggcg gcgcgttgat gatcaaggtg cccatggcct taagcccgga gatcgtcgcc
     1501 gggctggcca ggagggccgc atggaagacg acgatggcgt ccggcgactc aacgggggcg
     1561 ctcgtgcgga tcgggcggtt gaccgggccg aggcggacga aggaacgaac cggcgagccc
     1621 ttcttctccg aaccgtagga agagaaagcc gcgccgttca tcccgagacg cagaactgcc
     1681 gcgcctgcaa gcacttgccc ggccgcgtgg gcgccgagac ccccgatcga ctcgaacctg
     1741 acctcgaagt agccagctgg cagaacgctg cgatcggcga ccttggggtc catgactcac
     1801 ctcccagaag aactacggat tacgttgtcc taatgttgtc ctaatatagg gatcggacag
     1861 ggggcgtcca gcttcagctg cgaacggcga tgcaaggcga gggtaggcgc gggtctcctg
     1921 acccgtgcac tctccaaatt aaaaatccgc tcccagcgag aagaagtagc gcggcttgcc
     1981 ggtgtggtcg atggtggtgg gccaggcagt gtcgaaccgc agaatcaaaa agcctaaatt
     2041 cgcccgcgcg cccagcccga aactggcttt gatgtcgttg aagcgcagcc cgccgccttg
     2101 cgtgccccgg aaggtactgt tttcgtacca ggccgccccc atgtcgtaga aggccacccc
     2161 ctgcacctgg ctgatggtca aggggagcgg aaaacgtaac gccagaaagt ccaggaaggg
     2221 gtagcggagt tcccaatttg tgagtgcgta gtgggtgccg tcaaactggt agaaatcata
     2281 tccgcgcagg ggagtgacca cgctggccaa gtagaggtcg ttgatgctgt aaacccgatc
     2341 gacgttggcg ttggcaatcc gcgaaccgat ccagttggca gtcccgccca aaaagaaacg
     2401 cttggaagtg gaactggagg ccccccccgc cagccgcagg gtgaaggtat agcgcttgag
     2461 gaagtgccag tacttgcggt agtcggcctc caccgccacg taggagattc ccttgttggc
     2521 taacttcggc gcctgctcca cccgcacctg ccacctctgt ccgttggcgg ggccggtcag
     2581 cccccacaga gccgtgtcat tgatccacga gagaccgacg agggccacct gtgcgctttt
     2641 gtcgtcaaag ggcgggtcaa agtagcggcg gtcgatattg agcccggaaa acgaaagttc
     2701 caggcgcgtg aacttggtga acgggaaggc caccgaaccc aaaaagccgt acacccggtc
     2761 ggcgaagagc cggtccagag gatcgatgta gaagttgcgc gtgtgcaggg cccccactcc
     2821 gtaatcaatc cgcttgggcg agtagaagta gagcacctcg acgttgctct ggtcgatggt
     2881 gttgaacagg tcggtggcca caaagaactg gtggttggag aaaaggtcgg agaccaggag
     2941 gaacgactgg ccccgcagcc cgaagaaggt gctgtaaccg acgcttcccg aaatcagatc
     3001 gggcgaaaac ttcactttgt atttgtgcgg gcggtaggca cccgaaggga gcttcgactc
     3061 ctccagattg attttcgcca gcggcggaac gtaggcggtg tcgggcgcca gggtgctgtc
     3121 ctcggacacc cggctgacgt tcccggcccg gaagacgtag gaaggccctt ccccgccttt
     3181 ggccgccatg cgcggcgctt tcgccgccgc cgtgtcacgg gcgctatcgg ccggcggcgt
     3241 cggttcctct cggctggcaa agaagcgctt ctccccggct tgcgtacgga gggcgtaacc
     3301 ggtcagggac agtgcctcgt ggttttgcgt ggccggtttg atatcgctaa tgacgaaaat
     3361 gtcgaacccg cccttgtaga aactggaaag ggcgatctga ttcccttctt tcgaccagga
     3421 tggtgaaaaa cagccggtga ggacgttcgt gatcgggtaa tcccggccgc cattagctga
     3481 cggagacgga tcggctttca cgctggccac cggaggactg gccggctgtt ctaagtccag
     3541 aatgtagagg ttatcgatgc cgctgcggtt gttggagaag accaacttct tcccgtccgg
     3601 cgaccaggac ggcccccggt tgttcttgcc gtccttggtt aacggggtaa tctcatcaga
     3661 cgcaaggtcg tagaggaaga gattgtagtt tccgtacggc tgagcatctg cagtgccgtt
     3721 accggcggtt ggccggtcgg aggcaaaggc cagtgccttt ccatcgggcc gccaggccgg
     3781 atcctggtcg tcgtcggtat cgtcggtgag acgccgcagg cttttggttt cgagagtata
     3841 gacgtagaga tcggccttgc cgttgcgcat cccgacgaaa gcgattttgg aaccgtccgg
     3901 cgaccaggcg ggcgagagca tcccgtccat tttgaacttc agccgttttt cgactttgtg
     3961 gtttttcacc cggacgacca tcagggcgtc ttcgcccttg ctcttggaaa ccagtgcaat
     4021 ccgggtggcg tccggcgacc aggcgatgcc ggagacgtag gagtgcaaag actcaaagtc
     4081 gcccgagcgc tcccctttca ccacgcggtc gatgactttg ccgtccaccg ccgaaatgat
     4141 gtaaacctcg gtatagccgc ttttgtcgga aaaaacggcg atccggtcac cggtgggtgc
     4201 aaaggccggc ttctcgttga agtgggagcc gtctttttcg tgattggtga gggctttggc
     4261 gaagtctttc ggctcctgac gccgggcgat ttcgggccag tactccttct tgagcgcctt
     4321 ctgccaggcg tcgttgaact cctgctcgga aagcccgatc gactgcttga gtgccttgtc
     4381 catggtcaaa gccgctcgcc ctttgttcag cacctcggca attttatcct ggccatagcg
     4441 gtcggctaaa aacagaagcg ccgactgccc ttctttgtac gccagaaaac cgccggcata
     4501 ctccaatggt gccaggtagt tgtgaatggt ggcatcccgc agaaccatgt cggcctgggg
     4561 atcccagccg tgccgcgagg agtattcggc gtacccttcg gcaaaccaga gcgggaggcg
     4621 gaagaggtac tgccgcgaca agagcgagcc caagacgttc tggtagagca tgtcgaacac
     4681 cagggcgtgg gtcagctcat ggtgcagcac gtgccggaat tcttcgtacg atcccatgaa
     4741 cggcagcacc acgcggttct tgaacgactc ggtgaacccc cccacgcctt cttcaatcaa
     4801 ttcaggggtg acgttggttt gctgaaagtc gttgtgcgac tggtagagga tcaccggcac
     4861 ccggcgcctg agatcgtagt tcaactcgct ccggatttcg acgtaggcgc tctccagcgt
     4921 attggccgcc cacgaggcca ggtcgtaccc gtcctggtag tagtagacgt cgaagtggtc
     4981 ggtctggagg tacgaccagt cgaagttgcg gtagtgaact ttattcttgc cgaaaaatgg
     5041 ttcctgcgcc gagagccgcg ccgcgaaaaa gaaaaagagg cccacaccaa gcaccaggct
     5101 cctccaaccg cgctgtcgtg acataatcat gctcctcttc catccctcag acgagatggg
     5161 attgacatat ttctttggca gtgcgtggcg agtgtcggtc gaaatccgat tcattctact
     5221 cctaatatac gacaggcgat ggaaatcgtg tagcaccaga atcagcctct aacttccttt
     5281 ttaccacctg caaaccaacc atacagaacc ggcaacacca aaagggttaa ggtggtagaa
     5341 gtcaaaaggc ctccgaccac caccaccgcc agaggtttct gcacttccga gccgacaccg
     5401 gtagcaaaaa tgaggggagt taaggcaagc atggtcacca aagcggtcat cgagaccggg
     5461 cggagacgcc gggcacagcc ggttcgaatc gcctcttcga gcggcattcc ggactggcgc
     5521 agttgattga tgtaggaaat tagaacgact ccgtttagca ccgccacgcc gaagagggca
     5581 atgaaaccga ccgaggcagg aa
//
LOCUS       DBNGZD010000009         4108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_695486, whole genome shotgun sequence.
ACCESSION   DBNGZD010000009 DBNGZD010000000
VERSION     DBNGZD010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_695486"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="AC16GM_00220"
     CDS             complement(<1..562)
                     /locus_tag="AC16GM_00220"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmA/TRMU family protein"
                     /protein_id="HIR2922136.1"
                     /translation="MSEKAQKRILVAMSGGVDSTVTACLLKEAGHDVIGVTLKLWEYK
                     DVGGVEKTDGHCCSLESIEDARFAARKLGFPHYVFDYTVPFGQAVIDDFASEYLAGRT
                     PNPCVQCNSKIKWAPLLERALALNCDYLATGHYAQVAFDEDRERFVLRKASDLFRDQS
                     YFLWRLTQEQLARTLFPLGALPKAEVR"
     gene            669..1286
                     /locus_tag="AC16GM_00225"
     CDS             669..1286
                     /locus_tag="AC16GM_00225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2922137.1"
                     /translation="MKIVIFGASGKTGRWLVVQALDQGHDVTAFVRNPAAFRITNQKF
                     SKVQGDVMNPEQVGTAISGKEAVLSAIGFSRKSPKTVCADGTRNMIAAMQKHGVRRLI
                     CESAYGVGDTRNRGFYARFLSLIIQSRLADKEKMEAAVRQSGLDWVIARPTALTDGSK
                     TGGYRAGSNLQLGFWPMISRADVANFMLKQITDTTFLRQSPTVSY"
     gene            complement(1380..2543)
                     /gene="nifS"
                     /locus_tag="AC16GM_00230"
     CDS             complement(1380..2543)
                     /gene="nifS"
                     /locus_tag="AC16GM_00230"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HIR2922138.1"
                     /translation="MRTVYLDHNATTPVLPEVAEAMTQVFVNAFGNASSIHTFGREAK
                     SYLEEAREQVAKFIGGAPDEIYFTSGGTEADNLALKGVSNAYREKGKHIITSQIEHHA
                     VIHSGQHLSEEGFEVTYLPVDRYGLVDPDDLRKAIRPDTILVSIMQANNEVGTIQPIS
                     ELAEIAHAKGVLFHTDAVQALAKIPVNVNELGVDLLSISAHKVYGPKGTGALFIRKGI
                     DITPLLHGGSHERNRRAGTENVAGAVGFAKALEIAERDGEQERARQNTLAEYFRTEVE
                     KRIEDSVFNGHPTRRVPTTCNFSFKYVEGEAIIVNLDLEGIAVASGSACASGATEPSH
                     VLTAMHIHPDIAKSSVRFSLGRSNIREDIDYVLDVLPQVVQRLRAMSPLYVRT"
     gene            2673..3311
                     /locus_tag="AC16GM_00235"
     CDS             2673..3311
                     /locus_tag="AC16GM_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG domain-containing protein"
                     /protein_id="HIR2922139.1"
                     /translation="MNQQICNLLHQTVNRLEEFSFPFDQTAIPPNGIYILFEKGELAH
                     NTKRIVRIGTHTGENQLPSRLIQHFVNENKDRSIFRKNIGRCLLCKENDVFLQKWEID
                     LTTRKAKKRYGDSIDYEKLRMVERRVSEYIKRNFSFAVFKVEEKTKRVDLESKIISTV
                     SLCRDCFPSNQWLGLYSPKKKIKECGLWQVNELYKKPLSLKEANELRDYFCR"
     gene            complement(3437..>4108)
                     /locus_tag="AC16GM_00240"
     CDS             complement(3437..>4108)
                     /locus_tag="AC16GM_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR2922140.1"
                     /translation="QGCDYPMVCGATVIAIGPKNRYVTPGMARPGDVVILTKGAAIEA
                     TGLFAVTFPQLVAEKFGEDFSRKAQEIFWKMSVVEDAITAASVGTRKNGVTAMHDATE
                     CGVWGGLYEVAQASGVGMRVEKDKIAVLDEVEKICSHFGMDPYSSISEGTLIITAVPK
                     KAEKIVVALKRKKIDAAIIGEVTHKRLGMMVKEDGREHRLEHPRVDPFWEAFGKILAK
                     VQGRS"
BASE COUNT          953 a         1090 c         1096 g          969 t
ORIGIN      
        1 tccgaacttc tgctttggga agcgcaccca gagggaacaa cgtcctggct aactgctcct
       61 gcgtcagtct ccagaggaag tatgactgat cgcggaaaag gtcggaggcc ttccgcagaa
      121 cgaagcgctc gcggtcctcg tcgaaggcca cctgcgcgta atggccggtc gctaagtaat
      181 cgcaattcag ggctagggct cgctcaagca gcggggccca cttgattttc gaattgcact
      241 gcacgcaggg gttgggcgtc cgaccggcca gatattcaga ggcaaagtca tcaatcaccg
      301 cctggccgaa gggcacggtg taatcgaaaa cgtagtgagg aaatccgagt ttccttgcag
      361 cgaagcgagc gtcctcaatc gactccaacg aacagcagtg cccgtcggtc ttctccacgc
      421 cgccaacgtc tttgtactcc cagagtttga gggtgacgcc aatcacgtcg tgtccggctt
      481 ccttcaagag gcacgccgtg acggtggaat cgacgccgcc ggacatcgcg accaggatgc
      541 gcttttgggc tttctcactc atagcgttga caacgttccc aaagaaaaat acaaggccgg
      601 tgaaccgaaa tcaaatggct gagattcctt ttggttattc gtcattcctc ggactatatt
      661 gaccctctat gaaaattgtc atcttcgggg cttcgggcaa gaccgggagg tggctggtgg
      721 tgcaggccct ggatcagggg cacgacgtca ccgcatttgt gcgcaacccg gctgcctttc
      781 gcataactaa ccagaaattc tcgaaggttc agggtgatgt aatgaatcca gaacaggtgg
      841 gaacggcaat ttcaggaaaa gaggcggtcc tctccgctat tggttttagc agaaagtcgc
      901 cgaaaaccgt ttgcgccgac ggcacccgaa acatgatcgc ggcaatgcag aagcacggcg
      961 tgcggcgctt aatttgcgag tcggcctacg gcgtgggtga cacccggaac cgcggtttct
     1021 acgcccggtt tctctcgctc attatccaga gtcgcttggc tgacaaagag aaaatggaag
     1081 cggccgttcg tcagagcggc ctcgattggg taatcgcgcg tccgactgca ttgaccgatg
     1141 gttcaaagac cggcggctac cgggccggat caaacctgca actcggcttc tggcccatga
     1201 tttcgcgggc cgacgtggcc aatttcatgc tcaaacaaat caccgataca acatttctcc
     1261 ggcagtcacc gacggtgagc tattagaact ggatgggcag ctcactttcg ggcgaccgag
     1321 acggtcgccc tatggggaat tccgatttcg ttgtctgggt aacgtttttt ctgacctgat
     1381 tacgttctaa cgtaaagtgg tgacatcgcc cggaggcgct ggacgacctg cggcagaacg
     1441 tctaacacgt agtcgatgtc ttcgcggata ttagaacggc ccagggagaa gcgcactgaa
     1501 cttttggcga tgtcggggtg aatgtgcatc gcggtgagga cgtgtgatgg ttccgtcgcg
     1561 cccgatgcgc aggccgaacc ggaggccacg gcgatccctt ctaagtcaag gttaacgata
     1621 atcgcttcgc cttcgacgta cttgaacgaa aagttgcagg tggtgggcac ccgccgggtg
     1681 ggatggccgt tgaagactga gtcctctatg cgcttttcga cttcggtgcg gaagtattcg
     1741 gctaatgtat tctgacgcgc ccgttcctgt tccccgtcac gctccgcaat ttccagcgct
     1801 ttcgcaaaac ccaccgcacc ggcgacgttt tccgtccccg cccggcgatt gcgctcgtgg
     1861 ctgcctccgt gcagaagcgg tgtgatgtca atccccttcc gaatgaaaag cgcgccggtc
     1921 cctttcgggc cgtagacctt gtgcgccgag attgagagca aatcgacgcc taattcgttc
     1981 acgttaaccg gaatcttggc cagcgcttgc actgcgtcgg tgtgaaagag gactcctttg
     2041 gcgtgggcga tttcagccaa ctcggaaatc ggctgaatgg tgccgacttc gttatttgcc
     2101 tgcatgatcg agaccaaaat cgtgtccggg cggatggctt tccgcaggtc atccggatcg
     2161 accagcccgt agcggtccac cggcaggtag gtgacctcga acccctcctc ggagaggtgc
     2221 tggccgctgt ggattacggc atggtgctcg atctgggagg tgataatgtg cttgcctttt
     2281 tcccgataag cgttggaaac gcctttgagg gctaaattgt ccgcctccgt gcccccggag
     2341 gtaaagtaga tttcgtcggg cgccccgccg atgaacttcg ccacctgctc ccgcgcctct
     2401 tcaaggtagc tcttcgcctc ccggccaaag gtgtggatag atgaggcatt gccgaaggcg
     2461 ttgacaaaca cctgcgtcat cgcttcggcc acttcgggca gaaccggggt ggtggcgtta
     2521 tggtctaagt agacggtgcg catagtaatg atttttactt ccggtaacct tttgccttac
     2581 attatactat acggaattaa ttcaaagata ccaagtccaa gatcaatgaa taaattagtc
     2641 aatgggtggg gttagtcccc gtaggttata tcatgaatca acaaatttgc aatttgttac
     2701 atcaaacagt caataggtta gaagagttta gttttccatt cgatcaaacc gccatacctc
     2761 caaatggcat atacattctt tttgagaaag gcgaacttgc acataacaca aagagaatag
     2821 ttcggattgg aacccatacc ggtgaaaatc agttgccttc gaggctaatt cagcatttcg
     2881 tcaacgagaa taaggacagg agtatattta gaaagaacat tggaaggtgt ttattgtgca
     2941 aagaaaatga tgtatttctt caaaagtggg agatagacct taccacaaga aaagcaaaaa
     3001 agaggtatgg cgattcaatc gactatgaaa aattacgaat ggtcgagcgg cgagtttcgg
     3061 agtatataaa gcggaatttc agttttgcgg tttttaaggt tgaagaaaaa acgaaaagag
     3121 tagaccttga atcaaagatt atctcaactg tctctctgtg tagagactgc ttcccttcga
     3181 atcaatggct gggtttatat tcaccgaaaa agaaaattaa agaatgcggt ttgtggcaag
     3241 taaatgaact ttacaaaaag cctctttcgt tgaaagaagc caatgaatta cgcgactact
     3301 tttgtcgtta acttgagtgc cattgagatg agcctttatt gaacctggga gttaccgggt
     3361 tggcaaaatc cggaggttct ccgaaattgc agcggtctgt gaccgcctac cccaacgcta
     3421 cggcgcaatc tttaagttag cttcttcctt gcaccttagc taagattttt ccgaaggcct
     3481 cccagaacgg atcaacccgg ggatgctcca agcggtgctc ccggccgtct tctttcacca
     3541 tcatccccag acgcttgtga gtaacctcgc cgatgatggc ggcgtcaatt ttcttccgtt
     3601 tcagagcaac aactatcttc tctgcttttt ttgggacagc agtgatgatg agcgtccctt
     3661 cggaaatgga cgaatagggg tccatcccga agtgagagca aattttttcc acttcatcta
     3721 ataccgcaat tttgtccttc tcaacccgca tccccacgcc ggaggcctgg gccacttcgt
     3781 acagcccgcc ccagaccccg cactcggtgg cgtcgtgcat ggcggtcacc ccgttcttgc
     3841 gcgttcctac tgacgccgcc gtgatagcat cctccaccac cgacatcttc cagaaaatct
     3901 cctgcgcctt gcggctgaag tcttccccga acttctcggc tactaactga ggaaaggtta
     3961 ccgcaaaaag tccggtggcc tcaatcgccg cgcctttggt cagaatcaca acatcccccg
     4021 gccgggccat tcccggagtg acataccggt tctttggccc gatggcgatg acggtggccc
     4081 cgcagaccat cgggtaatcg catccctg
//
LOCUS       DBNGZD010000010         5972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_139834, whole genome shotgun sequence.
ACCESSION   DBNGZD010000010 DBNGZD010000000
VERSION     DBNGZD010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_139834"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="AC16GM_00245"
     CDS             <1..499
                     /locus_tag="AC16GM_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR2922141.1"
                     /translation="AADYPVIAIGKIEDLFAGQGVTQAVHTRDNAEVMQAMTQAVAET
                     GRGLIFANLVDFDTIWGHRNDVPAFAGGLERFDRHLGELIPRLRPDDLLIITADHGCD
                     PTTPSTDHSREYVPLLVYGQRLKSDVNLGTRSSFADVGATIAEIFRLPGTGNGKSFWQ
                     EIAAA"
     gene            462..869
                     /gene="cdd"
                     /locus_tag="AC16GM_00250"
     CDS             462..869
                     /gene="cdd"
                     /locus_tag="AC16GM_00250"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR2922142.1"
                     /translation="MAKVSGRKSPRPDRLFLAARHAFRRAYAPYSKFPVGAALEGKRG
                     EVFAGCNVENSSYGLTVCAERVALFSAIARGERSFTRLAIVTRSRTPVTPCGVCLQVL
                     AEFCDDLEIISATTSGKKATYRLRELFPKPFRR"
     gene            900..1697
                     /gene="deoC"
                     /locus_tag="AC16GM_00255"
     CDS             900..1697
                     /gene="deoC"
                     /locus_tag="AC16GM_00255"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR2922143.1"
                     /translation="MIEQVLLETWRTDLSRTLGIDFPPLSQNSGRSVAFSPAEFHSLP
                     APEKRKYLTGVSDYALLKPDLTSPVLEEACSRARELGCFGFCSLPYFVPQARKFLEGS
                     PVHLISVVGFPWGVHPPQTKQKETEWLAAEGVAEIDFVTNTSAIKKGDYATVWEEVRL
                     IRQTFPLGILKGIIECPLLTTEEKVKTAWLVEQAGCQFVKTGSGLFGKVQVADVELLR
                     RILLPATNIKAAGGIRTLEDALPLLEAGAERLGTSTPDTILAEISNA"
     gene            complement(1879..4335)
                     /locus_tag="AC16GM_00260"
     CDS             complement(1879..4335)
                     /locus_tag="AC16GM_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR2922144.1"
                     /translation="MASRFLCLLALAFLLTPATLFPFEPLFDTWLSYDVGVYPSAVIA
                     SDLDGDGDQDLVIPSYDGLFLFSVLKNNGDGTFAPRTDYRIGRYIYAVVASDLDRDGD
                     QDLAVTISDSNAVAVLMNYGDDTFAVRVNYDAGYRPEAISAVDIDDDGDVDLVIANTG
                     HTTNQGKTVSVLKNNGDGTFAPRVFYGTGNGPTSICAPDLDGDGHPDLVTADYFSRSI
                     SVLKNNGDGTFALKIDYATGNAPSAVFASDFDNDGDQDLAVTNDSSQSVSIFKNNGDG
                     TFAPKVDYETGNRPRSVFVADLDKDGDKDLAVANMLSNTVSLFLNNGDGTFAPKRDYV
                     VGWNPSSIFCADVDGNGSPDIVASSISSSTVSVLKNKGDGTFFVQTNYAAGDEPSSIL
                     AADLDRDGDQDLVVANSESSTVSVLKNNGDGTFAGRVDYGTGSKPISIFASDFDEDGS
                     QDLAVANAWSYTVSVLRNLGDGTFAEKVDYGTGSTPPFSVFASDLDGDGDPDLAVANR
                     GNTVSVFMNNGDGTFAPKIDYRTGTGPYAVLACDIDGDGDQDLVTADNTLDVGYLSVL
                     KNNGDGTFASPVNYPSAIEPNAIFASDLDRDGDQDLITAGWTVSVSMNNGDGTFPMKV
                     DYGRYYFSSIFVQDFDGDGDPDLVAPNSGNAVSIFKNKGDGSFSNRADYGTGNAPSAV
                     SASDFDKDGDIDIVTANYLGNSVSVLKNISTPTKVKDNGDKPLPSSWVLQQNHPNPFN
                     PSTIIRFAVPQPGNMTLEIFNLLGQRVKTLVNEVVSAGEKEVTWDGTDDRGKEVASGI
                     YFYRLKAEDFMETKKMVLFK"
     gene            complement(4470..>5972)
                     /locus_tag="AC16GM_00265"
     CDS             complement(4470..>5972)
                     /locus_tag="AC16GM_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2922145.1"
                     /translation="LLIWQINESQSQGNNPPKVDLLQADGLRNLENGNNRGDAGDPFP
                     GTSNNRNFDEFSNPNSKNPFNQAATQVAVFNIGNGGLDTMTANFEVTYGRPHLSLDST
                     WFDDAATGNNNGKIDSAETVRFYVRLKNAWANASNVTVLLRADDPRIVFADSTALLAS
                     VPGLGATTTSLSDPIEFTADPSLPNSYTIFFTVLAWNNDSSATVQQTFKATVGPTTIL
                     LVDDDGGTNNWESFYTTTLDSVRKPFGSHTVLTGGIPSIPTLSSYPTVIWFTGNNRTP
                     TTLTSTAVANLKAYLDGGGKLFITGQSIGEALAASADSSFLINYLHCRYGGNTFTIRA
                     NGVTGDPISNALDVYILGADGAANQTSADWLKLQPGADSIFTYLSNSKPAGVRVIKDT
                     TYKLVYLGFGFEGISSTRVGFATRQDVMTKVLNWLNAPYPVIILGDFDNSGVVDITDI
                     VGTVAYVVFGTPGPVNGQASIDVNCDGVIDLVDIVAMIQNVVFSLPFSCP"
BASE COUNT         1350 a         1627 c         1566 g         1429 t
ORIGIN      
        1 agctgcggac tatccggtca tcgccatagg caagatcgaa gacctctttg ccgggcaggg
       61 ggtaacgcag gcggtacaca cccgggacaa cgctgaagtg atgcaggcaa tgacgcaggc
      121 ggtggccgag acgggacgcg ggctcatctt tgccaatttg gtcgatttcg ataccatctg
      181 gggtcaccgc aacgacgtgc cggcttttgc cggaggtctg gagcgtttcg accgccactt
      241 aggcgaactc atcccccgcc tgaggccgga tgatctcttg attatcacgg ccgatcacgg
      301 ctgcgatccc accactcctt ccaccgacca ctcgcgggag tacgtgccgc ttttggtcta
      361 cggccagcgg ctcaaaagcg acgtcaactt gggaaccaga tcctcttttg cagacgtggg
      421 agcgaccata gcggaaatct ttcgtcttcc ggggaccggc aatggcaaaa gtttctggca
      481 ggaaatcgcc gcggcctgac cggctgtttc tggccgcccg gcacgccttc cgccgggcgt
      541 acgctcccta ctcgaagttt cccgtcgggg cggcgctgga ggggaaaagg ggggaggtct
      601 tcgcgggctg caatgtggaa aattcctcct acggcttgac ggtttgcgcg gagcgggtgg
      661 ctcttttctc cgcaattgct cggggtgaac gctcgtttac gcgcctggcc attgtcactc
      721 gcagccgtac tccggtgaca ccctgcggtg tctgcctgca ggttctggcc gaattctgcg
      781 acgatctgga aatcatctcg gccacgactt ccggcaaaaa ggcaacctac cggctgcgcg
      841 aactcttccc gaagccgttt cgccgctgat cccggtcgtt acccgccacg ggagttcatt
      901 tgatcgaaca agttctgctg gaaacctggc gcacagacct atcccgaact ctggggattg
      961 attttccacc gctttcccaa aattccggac ggtcggttgc attttctccc gccgagtttc
     1021 attctctccc ggctcccgaa aaacggaagt acctgaccgg cgtcagcgac tacgccctcc
     1081 tcaagcccga tctgacgtcg ccagtgctcg aggaggcttg ttcacgggcc cgggaattgg
     1141 ggtgctttgg cttctgcagc ttgccctact tcgtcccgca ggcgcggaaa tttctggagg
     1201 gctcgccggt gcacctcatc tccgtggttg gatttccctg gggcgttcac ccgccgcaga
     1261 cgaaacaaaa agaaacggaa tggctggcgg ccgaaggcgt ggctgaaatt gatttcgtca
     1321 ccaataccag tgccattaaa aaaggtgact acgcgacagt ctgggaagaa gtccggctga
     1381 tccggcaaac tttcccctta ggtatcctca agggaattat tgagtgtccg cttttgacca
     1441 ccgaggagaa agtcaaaacc gcctggctgg tggagcaagc cggctgccag ttcgtcaaga
     1501 ccggctccgg cctgttcggt aaagtgcagg tggcagacgt agaacttttg cgccggattc
     1561 ttttgcccgc aaccaatatc aaagccgctg gcggtattag aacactggag gatgcacttc
     1621 ctcttttaga agccggcgcc gaacggttgg gaacttcgac tccggatacc atccttgctg
     1681 agattagcaa cgcctaaccg gttgttcctt aaccgatttc accaattagc acaaggggaa
     1741 tattaggttg ttatgacccg tgaggtcatt actttggtgg aatgattcaa ctccctctcc
     1801 tgtagtggtt cgatccgcca taggcggact caccaccctg agcctgtcga tggggagagg
     1861 gttagggtga ggtcgatatt acttaaacaa caccattttc tttgtctcca taaaatcttc
     1921 tgccttcaac cgatagaagt aaatcccact ggccacttct ttgccccgat catccgtccc
     1981 gtcccaggtt acttcttttt ccccggcgga gaccacttca ttcactagcg tcttcaccct
     2041 ctgtcctaat agattgaaaa tctccaaggt catattcccc ggctggggta ctgcaaagcg
     2101 gatgatggtg ctgggattga aggggttcgg atgattctgc tgtaaaaccc aagaggaagg
     2161 caacggtttg tcaccattat cttttacctt ggtaggagta gatatgttct ttaagactga
     2221 gacggagttt cctaaataat tcgccgtcac tatgtcaatg tcgccgtctt tgtcgaagtc
     2281 cgaggctgaa acagcagagg gggcatttcc agttccgtaa tctgctctat tggaaaaaga
     2341 accgtccccc ttgtttttga agatcgaaac agcattgcca ctatttgggg caaccagatc
     2401 gggatctccg tctccatcaa aatcttggac aaaaatggaa gaaaaataat acctcccata
     2461 atccactttc attgggaacg tcccatctcc attgttcatt gataccgaaa cggtccatcc
     2521 ggcagttatc aagtcttggt cgccatctct gtctaaatct gaagcaaaaa ttgcgtttgg
     2581 ctcgatggct gagggatagt tgactggaga ggcgaaagtt ccgtctccgt tgttctttaa
     2641 taccgacaaa taaccgacgt ccaaagtatt gtctgcggtt accagatctt gatctccatc
     2701 accgtcgata tcgcaagcaa gaactgcgta aggccccgtt cctgtgcggt aatcaatctt
     2761 aggagcaaat gtaccgtctc cattattcat aaagacagaa acagtgttcc ctctattcgc
     2821 aacagccaaa tccggatcac catcaccgtc taagtctgaa gcaaaaaccg agaatggagg
     2881 agtgctccct gttccgtagt caactttttc agcaaaagtt ccatcgccca gatttctcaa
     2941 gaccgaaact gtgtagctcc acgcattcgc caccgccaaa tcttgacttc cgtcctcgtc
     3001 aaagtctgaa gcaaaaattg agattggctt acttccggtt ccgtaatcca ctcttccagc
     3061 aaacgtccca tctccattgt ttttgagaac cgaaacagta ctactttcgg aatttgctac
     3121 aaccaaatct tggtctccgt cccgatctaa atctgcggcc aaaattgagg aaggttcatc
     3181 tcccgcggca taattcgtct ggacaaaaaa agtgccgtcg cctttgtttt tcaataccga
     3241 gaccgtgcta ctgctaatac ttgacgcgac aatatctggg ctcccattac catccacatc
     3301 tgcacaaaag attgaagaag gattccaacc cacaacgtag tctcgtttgg gtgcaaaagt
     3361 tccgtccccg ttgttcaaaa agagagagac agtgttgctg agcatatttg ccaccgctaa
     3421 atccttatca ccatccttat ctaaatccgc aacaaagact gatcgaggcc gattccccgt
     3481 ttcatagtcg actttgggag cgaaagttcc gtcgccgttg ttcttgaaga ttgagacgct
     3541 ctggctgcta tcattggtca ctgctaaatc ctggtcacca tcgttatcga aatctgaagc
     3601 gaaaaccgca gaaggagcat ttccggttgc gtagtcaatc ttgagagcaa aagtgccatc
     3661 tccattgttc tttagaacgg aaatacttcg gctgaaataa tctgccgtaa ccaaatcggg
     3721 atggccgtct ccatctaaat ccggagcgca gatggaagta gggccgttcc ccgttccgta
     3781 aaaaaccctg ggagcgaatg ttccatcccc attgtttttt aggaccgaca cggttttgcc
     3841 ctgattggtt gtgtgtcctg tattagctat tactaaatct acgtccccgt cgtcgtctat
     3901 atcgacagca ctgatcgctt cgggtcgata tcctgcatcg taatttactc taaccgcaaa
     3961 agtgtcgtca ccatagttca ttaatactgc tacagcattg ctgtcagaaa tcgttactgc
     4021 caaatcttgg tctccgtctc tatctaagtc tgaagcgacg accgcgtaaa tatagcgccc
     4081 aatccggtaa tctgtcctag gagcaaaggt cccatcccca ttgtttttta atactgaaaa
     4141 tagaaagaga ccatcataac tcggtatcac caaatcttgg tcaccatcgc catctaaatc
     4201 cgaggcaata actgccgaag ggtagacgcc aacgtcgtag ctaagccacg tatcaaacaa
     4261 tggctcaaaa gggaacaacg tggcgggtgt caagagaaat gcgagtgcca aaaggcacaa
     4321 gaatcggctg gccattttcc ttcccctggc aataaatata tacgtattta ccgccgtcct
     4381 ttgactgctt ttcgcttttc cgcagtcttt ttgtcctaaa aacaaaaagc ccccgctaaa
     4441 ccgggggctt tttggctgaa gcgcactctt tacgggcagg agaaaggcag gctgaagacc
     4501 acgttctgga tcatcgccac gatgtcgaca aggtcaatga caccgtcgca gttcacgtca
     4561 atggaggcct gaccgttgac cggacccggc gtgccgaaga ccacgtacgc gacggtgccc
     4621 acaatgtcgg tgatgtccac gacgccgctg ttgtcaaaat cgcccaaaat gatcacgggg
     4681 tagggtgcgt tgagccagtt taagactttt gtcatcacgt cctgccgggt ggcaaaaccg
     4741 acgcgggtgc tggagatacc ctcaaagccg aaacccaggt aaaccagttt ataggtggtg
     4801 tccttgatca cccgcacgcc ggcgggtttg ctgttggaaa ggtaggtgaa gatgctgtcg
     4861 gctccgggct gcagtttcag ccagtccgcc gaggtctggt tcgccgcacc atcggcgccg
     4921 aggatgtaaa cgtccaacgc gttggagatc ggatcgcccg tcaccccatt ggcccggatg
     4981 gtgaaggtat tcccgccgta gcggcagtgg aggtagttaa tcagaaaact ggaatcggcg
     5041 ctggcggcga gcgcttcgcc gatgctctgc ccggtgatga agagtttccc gcccccgtcg
     5101 agatacgcct taagattggc caccgccgtg ctggtgaggg ttgtcggcgt ccggttgttc
     5161 ccggtgaacc agatcacagt ggggtaggag gaaagagtcg ggatggaagg aatcccgccg
     5221 gtgagcaccg tatgggagcc gaacggtttg cgcacactgt cgagggtcgt cgtgtagaac
     5281 gattcccagt tgttggtccc gccgtcgtca tccacgagca aaatcgtggt cgggcccacc
     5341 gtggctttga aggtttgctg caccgtcgcg ctgctgtcat tgttccaggc caggacggtg
     5401 aaaaagatcg tgtacgaatt gggcaagctg gggtcggcgg taaactcgat cggatcggag
     5461 agcgaggtgg tggtcgcccc caggccgggc acgctggcca gaagcgcggt gctgtcggca
     5521 aaaacgatcc gaggatcgtc cgcccgcagg agcactgtca cgttggaggc gttcgcccag
     5581 gcgtttttga gccggacgta aaagcgaacc gtctcggcag aatcgatctt gccgttgttg
     5641 ttgccggtcg cggcgtcgtc gaaccaggtg ctgtcgagtg aaaggtgcgg ccggccgtag
     5701 gtcacctcga agttggcggt catcgtgtct aagccgccgt tgccgatgtt aaaaaccgcc
     5761 acctgagtgg ccgcttggtt gaacgggttc ttgctgttgg ggttggaaaa ttcgtcaaaa
     5821 ttccggttgt tgcttgtccc ggggaaagga tcgcccgcat ccccgcggtt gttgccgttc
     5881 tctaaattcc gcagcccgtc ggcctgcagc aggtcaactt tcggcgggtt attcccctgg
     5941 ctctggcttt cattgatttg ccagatcaaa ag
//
LOCUS       DBNGZD010000011        12037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1112597, whole genome shotgun sequence.
ACCESSION   DBNGZD010000011 DBNGZD010000000
VERSION     DBNGZD010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1112597"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="AC16GM_00270"
     CDS             complement(<1..541)
                     /locus_tag="AC16GM_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006457752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2922146.1"
                     /translation="MPSAFRFFLRLSSAGTIFLVLFFSFSAVFGQAPVLLYQKNGAAA
                     FDFLGNSVAGAGDVNGDGRADFIVGAPFADPGGITNAGSAYVYSGFTGALLFQKNGAA
                     VGDLLGSSVAGAGYVNGDGLADFIVGAPRADPGGLTDAGSAFVYSGATGGLLYQKDGG
                     AAGDNLGTSVTSAGDVDGDG"
     gene            complement(1645..3393)
                     /locus_tag="AC16GM_00275"
     CDS             complement(1645..3393)
                     /locus_tag="AC16GM_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922147.1"
                     /translation="MNHRTELRRGFKILLVISAVAGNVAAQERTVVSFSHFKPGEIQA
                     AVLTVKEPAKLRIRALGAAWKEDGDLYAYGWILDLGSRKVVWEMDRDNTESVRRTHVR
                     EFDGTVKLNAGLYKVYYFAGRPFFFGGKTDINSWDDFWNLLGNIFSGERYDEKNWEKV
                     GSKFFLEAAAAGAVTLADGDPYEKRAIVALTKPGNSRSLSAGFSVGAEVKVKIYASGE
                     YDDNTGTLADYGWIIDAGTRKRVWEMDRHHVDPAGGAEKNVQFTGEIGLPRGNYVATF
                     ITDDSHAYGKWNALPPSDPDNYGLVILPAAEGERKLFAPYTDTYREPVVIQLVEVGDD
                     DYVSAGFTLDRGTELHIYALGEYDQGEDQLADYGWIEDAGTGKKVWEMTPDNVVPAGG
                     AEKNKKFDDVITLPRGSYVVNYLSDGSHSYRHWNSAAPPDPEHWGITISGFGKNFDPK
                     AVHPYEPKEAQGDILTQMIRLKDDEDRREKFSLTGPTQVRIYALGEGDEDEMYDYGWI
                     VNKRTGRTVWEMTWRNTEPAGGATKNRKYDDIVWLDKGEYEVHFVTDGSHAFGDWNAA
                     RPADPTHWGITITRVK"
     gene            complement(3552..4904)
                     /locus_tag="AC16GM_00280"
     CDS             complement(3552..4904)
                     /locus_tag="AC16GM_00280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2922148.1"
                     /translation="MIGKDRLLSTLQKVLKKAGADQAEAVFLGGESGLTRYANSTIHQ
                     NVTERNHKIYFRVALGKKIGVAATNSMTPDDLQLALGNALTIAKQQRANPDFTSFPAK
                     AKYPKVRTHFKATGAFTPKERALAVKKAIEKAARHNLQIYGSFATGEGELAVANSLGV
                     AAYQPLSNAALNLIAMGDSSSGYADGLSRDVREVNIDSIAETAIQKCLDSSNPKDFPP
                     GTYEVILEPAAVANLMEWMSFVSFGSKAFQEQTSCLAGKIGQKIMADSVTIYDDGGDE
                     GGAALPFDFEGVPKKKVVFVDRGVAKGVVYDTLAANREKKKSTGHALTPDSASEGAIP
                     VNIFIKGSTSSLKKMISNCERGLLVTRFHYINGLLDPPNAAMTGMTRDGTFLVEGGKI
                     KHGIKNLRFTESMVKAFSQVAEISRERNRVAAWWESIGCITTPAVRITRFDFTGKTDF
                     "
     gene            complement(4901..6343)
                     /locus_tag="AC16GM_00285"
     CDS             complement(4901..6343)
                     /locus_tag="AC16GM_00285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2922149.1"
                     /translation="MKEALLEGIAFLKKKNADYADIRWVKNEQEMILVRNGNVEGLSR
                     DVDQGFGVRVLAGGAWGFAASAVLTPAELKRVAQKALDVAKASALTLKQKVKLAEEQP
                     HVDRYQTPVKRDPLAVSIDEKIGLLIDLTTGLQKEPKVKVAEANLDFRKISKWFVSTE
                     GAQIEQIITQSGAGMVATAVAGAEVQRRSYPNSHRGDFANAGWEFIEQMRMRDQIERV
                     RREAAALLSAPACPATETTVVISAPQMALQVHESCGHPIELDRVLGTEISLAGGSFMT
                     TDKFGKLRYGSPKVNIVGDATIPEGLGSFGYDDEGVKAQRFDIIRTGLFCGYLTSRET
                     APVLNQKSNGTMRADGWNRIPLIRMTNINLEPGEGTLDDLIADTREGLFIDMNKAWSI
                     DDKRLNFQFGCEVAWEIKDGKLGQMYRNPTYTGITPEFWNSCDAVCNRNHWKLWGVPN
                     CGKGEPMQVARVAHGAAPARFRRVRVGVSA"
     gene            complement(6429..7508)
                     /gene="waaF"
                     /locus_tag="AC16GM_00290"
     CDS             complement(6429..7508)
                     /gene="waaF"
                     /locus_tag="AC16GM_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR2922150.1"
                     /translation="MSARSLEPTKIKKIVVRAPNWVGDVVMSLPFFAQLRDIFLYAEI
                     SCLVKSHLAELYQHQPFISRVIPLAERANRLAVIFKTSQEIRRKKFDLAICLPNSFSS
                     AMIFFLAGIPRRVGYAAEGRGLLLTDRFPYLPDKKMIHQSEIYTNLLRAFGFQSDSDY
                     LPVLTAPPSVQKEVDRLFEKHRFTQKQRLAVIAPGSRAPARQWFPNRFAKLADRLIAE
                     GQTVLFVGGPADVPTAVEVKAKMKQKPIDLSGQTSLLGLAEVCRRSRVFIGNDSGAAH
                     VAAAAGTHVVVITGPGDPEQITPLTNRKTVLFHKIFCSHCRKNHCWRSDLPQECLRLI
                     SVDEVLETVWPILESTKVPQKIPAG"
     gene            complement(7505..9034)
                     /locus_tag="AC16GM_00295"
     CDS             complement(7505..9034)
                     /locus_tag="AC16GM_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2922151.1"
                     /translation="MSRNTVASSQGPVARKKSRQTLGQCLNATLVIALLLVVPLFAAI
                     AQEPVKLTLSECIQMALKQGKEAQIARSELEAAKSTYQARRLGCYLPRVNFQLTTPSY
                     QNSQSLQNFFGATERVLSKSTSLYFNGGVVLSQNLLTGGTLEVTGRANSLRNTANIFS
                     DFRERSGELLLSLNQPLFAASPHRNDLHQAEFAEKRAQWQYHERVWGLYRDVMQAYVD
                     LLAAGIEADVAGLELEKARMAARDAEEKKTQNLVDEKEVLTARAKTLEQEVVQLDALR
                     KREEKRLALFGLLGMKSEAAVELSGQIVPEAVPADRLEWYRSNASLTLDAKRAALSVD
                     EKKTALEQTRSQGGLGGTLSGSADIGRGKEKRDVTNNINTNRYGVSLTLTYPLWDGGA
                     QAAAERAAEVAYETVKLDREKQIQAAVTKVTSAINKVTTGQKRVELLGRIAELERQRL
                     ARAEEQQGQGLLSAKALADLKISALDAQTKYLQAVADHNAAVYELHQSFLEDTPPEIP
                     R"
     gene            complement(9027..10328)
                     /locus_tag="AC16GM_00300"
     CDS             complement(9027..10328)
                     /locus_tag="AC16GM_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2922152.1"
                     /translation="MDYREVVQVALDSIVTHKLRSALTTLGVVFGVAAVIAMLSIGEG
                     AKQEALEQIALLGTNNILIKATTPPKSSGSEEVEGRSEGLSLADARNIEALSHLVTAV
                     VPERWEQIDRANFGDKESKVEVIATTPDYLALTNQRLRRGSFFSAEDNTEGRRVCVIG
                     SEVKRNLFAFDDPVGKPIKLGDDYYTVLGVTEEKSIGKPKVEGLEVKNVNQQVYLPLG
                     TAQNRLERGGGGEGFYAFGGVTIFSSEGGGTKASEVDVLTVKVSDKEQIQPVAHVIKK
                     LLERRHRGVADFEIVIPEALLYQSQKTQRIFNIVMGAIAGISLLVGGIGIMNIMLASV
                     YERTREIGVRRAVGARRNDVLRQFLVEAVAISFVGCLLGLILGFGLARSVSYYAKWRT
                     IISLFSIVLAVGVSALVGIAFGYYPARRAAGLDPIEALRYE"
     gene            complement(10402..11010)
                     /locus_tag="AC16GM_00305"
     CDS             complement(10402..11010)
                     /locus_tag="AC16GM_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922153.1"
                     /translation="MSEEREEIQSITCRACREINTSGAQFCAHCGRSLEEKSDVDKKQ
                     ISEGTVGGVIFIVVLIFGLIYVLFGHSSKDSDSSVKPSTPFSSETTTTPSLSSSSPVK
                     KLGFVPTTEQVKEARRIVHKAEEVGLVYKIDLQLQSVWVDSDLWNLLTYDVKEVYALS
                     FAYFISDENSTTKNISWIGIRDKYTGKEIASYGSTWGFRVKN"
     gene            complement(11141..>12037)
                     /locus_tag="AC16GM_00310"
     CDS             complement(11141..>12037)
                     /locus_tag="AC16GM_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2922154.1"
                     /translation="QANLKIAQANLNRALADLELQKQQLELDLEKAERNAKEKAYEAP
                     AIKQEAEKELELARLKKETQLKLLEADIEKLRVEVDRAKDKVTTAQNDLAQMTLKAPI
                     PGLVVYMDIWKGNQMAKVQEGDSPWPGQELINLPDLSAMQVKTSVSEVDVDKLKLGQE
                     TNIKLDAYPEPTFHGKVTKISTLARRKDWNSRINVFDVEVTLDSADPKMKPGMSAKAD
                     IVVEKIPNSIWVPIEAVFEKEGKTVVYLMGSRSKKVEVEVGKRSDTHVAITKGLKGGE
                     KVALVDPTQKLEMLGVKKEEKK"
BASE COUNT         2418 a         3581 c         3192 g         2846 t
ORIGIN      
        1 cgccgtcccc gtccacatct cccgccgagg tcaccgaggt gcctagattg tcaccggccg
       61 cgccgccatc tttctgatag agcaggccgc cggtggcgcc ggaatagaca aaagccgagc
      121 cggcatcagt aagcccgccc gggtcggcac ggggagcgcc gacgatgaaa tcggcgagtc
      181 cgtctccatt cacatacccg gccccggcca cggaggagcc cagaaggtca cctactgccg
      241 cgccattttt ctggaaaagc agggcacccg tgaagccgga atagacataa gccgagccgg
      301 cattcgtaat cccgcccggg tcggcgaaag gagcgccgac gatgaaatcg gcccgcccgt
      361 cgccattgac atccccggcc ccggcaactg aattgcccag aaagtcaaag gccgccgccc
      421 catttttctg gtagagcagc accggcgcct ggccgaaaac ggctgaaaaa gaaaaaaaca
      481 gaacgaggaa aattgtccct gctgaactga gccggagaaa aaaacggaaa gcactcggca
      541 tacgacctcc gcactcgcta cggctcattt cgagaacgag tcgttcgcga tggttaaatc
      601 actggttttg tacttctaca gaaagtgaag gtacacgcac ctggtgacgg atgaagttac
      661 actggttgct cggacggaaa atgtaattcc ggtctttaac taatccggaa ctaaaacttg
      721 cccgcacctc cttatggtgg tcggctcccc cgacaatgga agaatagccg accatctatt
      781 cttgtcaagg acttttttcg tccgaaataa aaaagtgccc ataccgggag cgcttacctt
      841 ctcccaaaaa gcaatcctcg atcactgtca ctcattatta atcaatggct tactcggagt
      901 tagcgcgagg gtaaaaaggt tccttctgta ggcgggactt cagtcccgcc aaaagggacg
      961 gcactgagag tgccgcctcc gcgatgggcg ggctgaattt tggagtcgtc gcgggggcgc
     1021 acaggcaccc cttcccccgt cgaatttggg atcatcatag gggtaaaacg cccccccctt
     1081 tcccataggc gaggcttctc gatagagccg taccggccag cctcgcagaa atttggggtc
     1141 acgacggggg taaaacggac aatgtgcgaa atagtaggag cacatcagga gccagtggcc
     1201 agtagccagg ggccaaaatc cgggactcct tactcctgag aggccgaggt gctgtatatg
     1261 tctttttgtc ttgccctcaa tactcagttc tcatccgcct catgcggact ccgttctgcc
     1321 ttttcttcgg ctccctctgc ttcatctgct gtgaatctac tgatccccca tctccaatcc
     1381 cagatccctg atctctgctc tattccccgc ccccttctcg gaactccact atccttcagg
     1441 acaacaaatg attacaagca atcacaatcc gaaaatgcgc cgtgttgctc catggaactt
     1501 cttctctttc aacagggcaa aagctaaaaa aaattacgtg ccgccccctt cccggaacct
     1561 atgaacttta cctcgacacc ccccttcgac ttcgctcagg gcggtgagct tgtcgaaccg
     1621 cgacacctcg acacttcgac acttttattt tactcgcgta atcgtaatcc cccagtgggt
     1681 ggggtcagcc ggtctcgccg cgttccagtc gccgaaagcg tgggagccat cggtgacgaa
     1741 gtggacttcg tactccccct tgtccagcca gacgatgtcg tcgtacttgc ggtttttcgt
     1801 ggcgccgccg gcgggctctg tgttgcgcca ggtcatctcc cagaccgtcc gcccggtgcg
     1861 tttgttgacg atccagccgt agtcgtacat ctcgtcttca tccccctcgc cgagagcgta
     1921 aatgcgcacc tgggttggcc cggtgaggga aaatttttcg cggcggtcct cgtcatcttt
     1981 cagccggatc atctgcgtca gaatgtcacc ctgggcctct ttgggctcgt aggggtggac
     2041 ggcctttgga tcgaaatttt tcccgaagcc ggaaatggtg atgccccagt gttcgggatc
     2101 gggcggagcg gctgaattcc agtgccggta ggagtgggag ccgtcggaga gatagttgac
     2161 cacgtaactg ccgcgcggga gcgtgatgac atcgtcgaat tttttgttct tctccgcccc
     2221 gccggccggc acgacgttgt ccggggtcat ctcccagact ttctttccag tgcccgcgtc
     2281 ctcaatccag ccgtaatcgg ccagttggtc ctcgccctgg tcatactcgc ccaaggcgta
     2341 gatgtggagt tcggtgcctc gatcaagcgt gaacccggcc gaaacgtaat catcgtcacc
     2401 cacctccacc agttggatga ccaccggctc ccggtaggtg tcggtgtacg gggcaaagag
     2461 ctttcgctca ccttcggctg cggggaggat gacaaggccg tagttgtcgg gatcggaggg
     2521 cgggagcgcg ttccacttgc cgtatgcgtg gctgtcgtcg gtgatgaagg tggcgacgta
     2581 gttccccctg ggcaggccaa tctcgccggt gaattgcacg tttttctcgg ccccgcccgc
     2641 cggatcgacg tgatgccggt ccatttccca gacccttttg cgcgtgccgg cgtcaatgat
     2701 ccagccgtaa tcggccaatg ttccggtgtt atcgtcgtac tcgccgctgg cgtaaatttt
     2761 taccttcact tcagcgccca ccgaaaatcc ggcggaaagg gaacgggaat tgcccggctt
     2821 agtcagcgcg acgatcgccc gcttctcgta cggatcgccg tcggccagtg taacggcgcc
     2881 ggcagccgct gcttcaagaa agaatttgct tccgactttc tcccagttct tctcgtcgta
     2941 tcgttctccg gaaaaaatgt ttcccagaag attccagaag tcgtcccagg agttgatatc
     3001 cgtcttgccc ccgaaaaaga acggccgacc cgcaaagtag tagactttgt acagcccggc
     3061 attcagcttc acggtgccgt caaattcgcg cacgtgagta cggcgcaccg attcagtatt
     3121 gtcccggtcc atttcccaga ccacctttcg ggacccgaga tcgagaatcc acccgtaggc
     3181 gtacaggtcg ccgtcttcct tccaggccgc acccaaggcg cgaatgcgca acttggcggg
     3241 ctccttcacc gtgagcaccg ccgcttgaat ttcccccggt ttgaaatggg aaaaagaaac
     3301 cacggtccgc tcctgcgccg ccacgttgcc ggccacagcc gagatcacca ggaggatttt
     3361 gaaaccccgc cgcaattcgg tccggtgatt catagagata aagtcaccca tcggcccaag
     3421 gacatcttgc gcctggtttt ctttacgaga ggagaggaaa aaggttgcaa ttttttgtgc
     3481 ggccgtacat tacccgagaa gaccaagtct ccacccggcc gatagggact tggggttgcc
     3541 gggaagagct gttagaagtc ggtctttccc gtaaagtcaa atcgggtaat gcgtaccgcc
     3601 ggggtggtga tgcagccgat cgattcccac caggcagcca cccggttccg ctcgcgggaa
     3661 atctccgcca cctgcgagaa ggccttgacc atgctctcgg tgaagcgcag gtttttgatc
     3721 ccgtgcttga tttttccgcc ctcgaccaga aacgttccgt cacgggtcat acccgtcatg
     3781 gcggcattcg gcggatccaa aaggccgttg atgtagtgga agcgcgtcac cagcagtccc
     3841 cgctcacagt tggagatcat ctttttgagc gaagaggtac tgcctttaat aaagatattc
     3901 accggaatag ccccttcact ggcactgtcc ggcgtcaggg cgtggccggt ggattttttc
     3961 ttctcgcggt tagccgccag cgtgtcgtag accactcctt tggcgacacc gcggtccacg
     4021 aagacgactt ttttcttcgg cacgccctcg aaatcaaacg gcagcgccgc gccgccctcg
     4081 tcgccgccgt cgtcgtaaat cgtaacgctg tcggccataa tcttctggcc gatttttccc
     4141 gccaggcagc tggtctgttc ctgaaacgcc ttggagccga aggaaacaaa actcatccac
     4201 tccatcaaat tggccactgc cgccggctcc agaatcacct cgtaggtccc cggcggaaaa
     4261 tctttcgggt tgctgctatc gaggcacttc tggatggcgg tttcggcaat cgaatcgatg
     4321 ttgacctccc gcacatcgcg ggaaagtccg tcggcgtaac cggaactgga gtcccccatg
     4381 gcgatgagat tcagcgcggc gttggagagc ggctggtagg ctgccactcc gagcgagttc
     4441 gctaccgcca gctccccttc gccggtggca aaggaaccgt agatttgcag gttatgccgg
     4501 gccgcttttt cgattgcctt ctttaccgcc agcgcacgct ccttgggagt aaaagcgccg
     4561 gttgctttga aatgggtgcg gacttttgga tacttcgctt tcgcaggaaa ggaggtgaaa
     4621 tcgggattgg ctcgctgctg tttggcaatg gtgagcgcgt tgccgagtgc cagttgcaaa
     4681 tcgtcagggg tcattgagtt ggtcgcggcg acgccgatct tcttccccag cgctacccgg
     4741 aagtagatct tgtgattgcg ttcggtgacg ttctggtgaa tcgtggagtt ggcgtagcgg
     4801 gtgaggccgc tctcaccgcc taagaaaact gcttccgcct ggtcggcccc ggccttcttg
     4861 agaactttct gtaacgtgga aaggaggcgg tctttgccga tcatgcgctc acccccaccc
     4921 gcaccctgcg gaagcgggcc ggcgccgccc catgggccac ccgcgccacc tgcatcggtt
     4981 cccctttgcc gcagttcggc acaccccaca gcttccagtg attgcggtta cagaccgcat
     5041 cgcaggaatt ccagaactcc ggagtaatgc cggtgtaggt cgggttcctg tacatctgtc
     5101 ccaatttccc gtctttgatt tcccaggcca cttcacaccc gaactggaag ttcagccgct
     5161 tgtcgtcaat cgaccaagcc ttgttcatgt cgatgaagag tccttctctg gtgtcggcga
     5221 tcaagtcgtc caacgtccct tcgcccggct cgaggttgat gttggtcatc cggatgagcg
     5281 ggatccggtt ccaaccgtcg gcccgcatgg tgccgttcga tttctgattg agcaccggtg
     5341 cggtctcccg cgaggtgagg tagccgcaga ataatccggt gcggatgatg tcgaagcgct
     5401 gcgctttcac tccttcgtcg tcatagccga aacttcccag tccctcgggg atggtcgcgt
     5461 caccgacgat gttcaccttc ggcgaaccgt agcgcagttt gccgaacttg tctgtagtca
     5521 tgaaactgcc gccggctaaa ctgatttcgg tacccagcac ccggtcgagt tcaatcgggt
     5581 gaccgcacga ctcgtgcacc tgcagcgcca tctgcggcgc ggagatgacg accgttgttt
     5641 cggtggccgg acaggcaggc gcggacaaaa gcgccgccgc ttcgcggcgc acccgttcaa
     5701 tctgatcgcg cattcgcatc tgttcgatga actcccaccc tgcgttggcg aaatcgcccc
     5761 ggtgggaatt cgggtaggag cgccgctgca cttcggcccc cgcgaccgcc gtcgccacca
     5821 tccccgcccc cgactgggta atgatctgct cgatctgcgc cccctcggtg gagacgaacc
     5881 acttgctgat tttgcggaag tccaaattcg cttcggcgac tttgactttc ggctcctttt
     5941 gcaaaccggt ggtaagatct atcaaaagac caatcttctc atcaatgctg acggccaagg
     6001 gatcgcgttt tactggagtc tggtagcggt caacgtgggg ttgctcttcg gccagtttca
     6061 ctttttgttt aagcgtcagt gcgctggctt tggcgacgtc taacgccttc tgcgccacac
     6121 gctttaactc ggcgggagtg aggaccgcgc tggccgcgaa cccccaggcc ccgccggcta
     6181 aaacccgcac gccaaaaccc tgatctacgt cgcgggagag cccctctacg ttaccgttgc
     6241 ggactaaaat catctcctgc tcgtttttca cccagcggat gtcggcgtaa tcggcattct
     6301 tttttttcag aaaagcaatt ccctctaaaa gcgcttcttt caccttacct ccttggtaaa
     6361 gaaaattact tagacaaaac gtggcgtaac ttatgtgccc catccgccgc tgtcaaacga
     6421 ctcctccgct atcctgccgg tatcttttgc ggtacttttg tggactcaag gattggccac
     6481 actgtctcca gcacttcgtc aacggaaatc aggcgcaggc actcctgggg caggtcactc
     6541 ctccagcagt gattcttccg gcagtgtgag caaaatattt tatgaaagag aaccgtcttg
     6601 cggttggtga gcggggtgat ttgttctgga tcacccggac cggtgatgac caccacgtgc
     6661 gtcccggctg ccgcggccac gtgtgcggcg cccgagtcgt tgccgatgaa aacccggctg
     6721 cgccggcaaa cttcggccag acccaaaagc gaggtctgcc cggagaggtc gatgggcttc
     6781 tgcttcatct tcgctttcac ttccaccgca gtgggaacat ccgccggccc gcccacgaag
     6841 agtaccgttt gtccttctgc gatcagccga tcagccagtt ttgcgaatcg gttgggaaac
     6901 cactgccgcg cgggcgcccg ggagccgggg gcgattacgg ccagccgttg cttttgggtg
     6961 aaccggtgtt tctcgaagag acgatcaact tctttttgga cggagggagg ggcggtcaga
     7021 accgggaggt aatcgctgtc tgactggaat ccaaacgctc gcagtagatt cgtgtaaatt
     7081 tcactctggt gaatcatctt cttatccggc aggtaaggga accggtcagt caggagtaaa
     7141 ccgcgtccct cggcagcata ccctacccgc ctggggatgc ccgccagaaa gaagatcata
     7201 gccgacgaaa acgaattggg cagacagatg gccagatcaa atttcttgcg acgaatctcc
     7261 tgagaagtct tgaaaatcac ggccaatcga ttggcccgct cagctaaagg aatcacgcga
     7321 gaaataaacg gttgatgttg gtaaagctca gccaaatgtg atttcactaa gcagctaatc
     7381 tcggcgtaga gaaagatgtc ccgcagttgg gcaaagaaag gaagcgacat taccacgtcg
     7441 cccacccagt tcggcgcgcg cacaactatt ttttttatct tcgttggttc gagtgagcgg
     7501 gcactcaccg tgggatttcc ggtggggtgt cttcgaggaa actttgatgc agttcgtaca
     7561 ccgcggcgtt atggtcggcc accgcctgca ggtacttggt ctgcgcgtcg agggcgctga
     7621 ttttgaggtc ggccaaggcc ttggctgaga gaagcccctg cccctgctgc tcttcggccc
     7681 gcgccagacg ctggcgttcc agttcggcaa tgcggcccaa tagttccact ctcttttgtc
     7741 cggtagtgac tttatttatc gcgctggtca ctttggttac ggctgcttga atctgttttt
     7801 cgcggtcgag tttcaccgtc tcgtacgcca cctccgccgc gcgttcggcc gccgcctggg
     7861 cgccaccgtc ccagagcggg taggtcaagg tcaaagacac gccgtaccgg ttcgtgttga
     7921 tattgttggt aacatcccgc ttttcctttc cccgcccaat gtcagccgag ccgctgagcg
     7981 ttccgcccaa accgccctgc gaacgggttt gctccagagc cgttttcttc tcgtctacgg
     8041 aaagagctgc gcgcttggcg tcgagagtca gcgaagcatt gctccggtac cactccagcc
     8101 ggtcggcggg aacggcttcc ggtacaatct gaccggataa ttcaacggcc gcttcgcttt
     8161 tcatacccag cagaccgaac agcgccagcc gcttttcttc ccttttccgg agggcgtcca
     8221 gctgaactac ttcctgttcc agtgttttgg ctctggcggt aagcacctct ttttcatcca
     8281 ccaagttctg cgtctttttt tcttcggcgt cgcgggccgc cattcgtgcc ttttctaatt
     8341 ccaggccggc cacatccgct tcaatgcctg cggcgagaag gtcaacatat gcctgcatca
     8401 cgtcgcgata cagaccccat actctctcgt ggtattgcca ctgcgcacgt ttttccgcga
     8461 actccgcctg gtgcaggtca tttcggtgcg gcgaagcggc aaacagcggc tggttcaagg
     8521 agagcaggag ctctccgctc cgttcgcgaa agtcggagaa gatgttggcc gtgttgcgga
     8581 gcgaattggc ccggccggtg acttccaggg tcccgccggt caagaggttc tgcgaaagca
     8641 ccaccccgcc gttgaaatat aaactggtgc tcttggagag cacccgctcc gtagcgccga
     8701 agaaattctg caaactctga ctgttctgat aggaaggagt cgttaactgg aaatttactc
     8761 tgggaagata acaccccaag cgccgtgcct ggtaggtcga tttggccgct tccaattcgc
     8821 tcctggcaat ttgcgcttcc ttgccctgct tcagcgccat ctgaatgcat tcggaaagtg
     8881 tgagcttgac cggctcctgt gcaattgcgg caaaaagtgg aaccaccagg agtaacgcaa
     8941 ttaccagcgt tgcattgagg cattgcccca atgtctggcg actcttcttt ctggctaccg
     9001 gcccctggct actggctact gtattcctac tcataccgca gcgcctcaat cgggtccaaa
     9061 ccggctgccc gtcgggccgg gtagtagccg aaggcaatgc ccaccagagc ggagacgccc
     9121 accgcaagaa caatggagaa aagtgagatg atagtccgcc acttggcata gtacgaaacc
     9181 gagcgggcga ggccgaagcc cagaatcaat cccagaagac agccgacgaa actgatcgcc
     9241 accgcttcca ccagaaactg ccgcaggacg tcgttcctgc gcgcgcccac ggcccggcgg
     9301 acgccgattt cccgcgtccg ttcgtagacc gacgccagca tgatgttcat aatgccgatg
     9361 ccgcccacca gaagcgaaat cccggcgatg gcgcccatca cgatgttgaa aatcctttgc
     9421 gttttctggc tctggtacag aagcgcctcg gggatgacga tttcgaagtc ggccacgccc
     9481 cgatgccggc gttccaacaa ttttttgatg acgtgcgcca ccggctgaat ttgttctttg
     9541 tcgctcactt taacggtgag cacatctact tcacttgctt ttgtccctcc gccttcactg
     9601 gagaaaatgg tgaccccgcc gaaggcgtag aacccttctc ccccgccgcc gcgctccagg
     9661 cggttttgcg ccgtgcccag cggtaggtag acctgctggt tgacgttttt gacctccagc
     9721 ccttcgactt tcggcttgcc gatagacttt tcttcggtga caccgaggac ggtgtagtag
     9781 tcatctccca acttgattgg ctttcccacc gggtcatcaa atgcgaagag attccgcttg
     9841 acttccgagc caatgacgca gacccgccgc ccctccgtgt tatcctcggc agaaaagaaa
     9901 ctcccccggc gcaggcgctg gttggtcaag gcgagataat cgggtgtggt ggcgatgact
     9961 tccactttcg attctttgtc gccgaagttt gcccggtcga tctgctccca tcgctccggc
    10021 accacagcgg tgacgaggtg gctcagcgcc tcaatattcc gcgcatcggc taaagaaagc
    10081 ccttcagagc ggccttccac ctcttcgctg ccggatgatt tgggcggagt cgtggctttg
    10141 atgagaatgt tattggtgcc caaaagtgcg atttgctcta acgcctcttg cttggccccc
    10201 tcgccaatgg aaagcatcgc aatcactgcc gccacgccga agaccacacc cagagtggta
    10261 agcgccgagc gcagcttgtg cgtgacgatg gagtcaagcg caacctgaac gacttcgcgg
    10321 tagtccataa aaccttttca ccacggagac acagagaaca cgtcaaaaat tttaactatt
    10381 ctccgttcta tggttactac ctcagttctt cactctaaat ccccaggtac ttccatacga
    10441 agctatttct tttcctgtat atttgtctct aatgccaatc cacgaaatat tttttgtcgt
    10501 ggaattttca tctgaaatga aataagcaaa acttaatgcg tatacttctt ttacgtcata
    10561 agttaacaaa ttccacaaat cagaatccac ccatactgat tgcaattgca aatctatttt
    10621 gtatactagt cctacttctt ctgctttgtg cactattctt cttgcttctt ttacttgctc
    10681 agttgttggt acaaatccca acttttttac aggagaggaa gaagataagg aaggagtggt
    10741 cgtggtctcg gacgaaaaag gtgtagaagg cttgacggaa gaatctgaat ccttactaga
    10801 gtgaccaaac aatacgtaga tcaaaccaaa aataagaact actatgaaaa tcactcctcc
    10861 tactgtacct tccgatattt gttttttatc tacatcactc ttttcttcta gggaacgacc
    10921 acagtgcgca caaaattgag cccccgaagt atttatttcc cgacaggcac gacacgtaat
    10981 actctgaatt tcctcacgct cctctgacat cgtatatcct actttatcaa ttcaatcccc
    11041 tttgcttcca ttccactttc cttcagggtg cctttcgaat gaaattatac caatcttaat
    11101 tgtaataaaa cattctcagg tccttgcttg ggtccctttc ttatttcttt tcctccttct
    11161 tcacacccag catttccaat ttctgcgtcg ggtcaaccag ggcaactttt tctccgcctt
    11221 tcaggccctt cgtaatcgcc acgtgggtat cggagcgctt gcccacttca acttcgacct
    11281 tcttcgagcg ggagcccatc aggtagacta cggttttgcc ttccttttca aacaccgctt
    11341 caatgggcac ccagatcgag ttggggattt tctccacgac aatgtccgcc ttggccgaca
    11401 tccccggctt catcttcggg tcggcggagt cgagagtgac ttcgacgtcg aagacgttga
    11461 tgcgcgagtt ccagtctttc cgccgcgcca gcgtgctgat tttggtcacc ttcccgtgaa
    11521 aggtcggctc cgggtaggcg tctaacttga tgttcgtctc ctgccctaat ttcagtttgt
    11581 ccacgtccac ttcggagacc gaagttttga cctgcatggc ggagagatcg gggaggttga
    11641 ttaactcctg gccgggccag ggggagtcac cctcctgcac cttggccatc tggtttcctt
    11701 tccagatatc catatagacc accagcccgg gaatgggcgc tttgagggtc atctgggcca
    11761 ggtcgttctg agcggtggtg actttgtctt tggcgcggtc aacttccacg cgcagttttt
    11821 cgatgtcggc ttccagaagt ttcaactggg tttctttttt caggcgggcg agttccaact
    11881 ctttttcggc ctcctgcttg atggccggag cttcgtaggc tttctccttg gcgttccgct
    11941 cggctttttc gaggtccaac tccagctgct gcttctggag ttccaaatcg gccagggcgc
    12001 ggttgagatt ggcctgggcg attttcaggt tggcctg
//
LOCUS       DBNGZD010000012         9001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_834943, whole genome shotgun sequence.
ACCESSION   DBNGZD010000012 DBNGZD010000000
VERSION     DBNGZD010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_834943"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="AC16GM_00315"
     CDS             <1..1096
                     /locus_tag="AC16GM_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HIR2922155.1"
                     /translation="LASIVPYSLLVRTGEWHKKILKNNTLLFIATLPPDLFNPYSSFN
                     TAIVVFQKGIPHLSKPVFFCKVSNDGFKLKKNTRVSRPGTQLEQILKAYSTKHDIPEV
                     CAFRPISSESEEWSPEAYIENAPHSDEEFVKGFEDSVKNQASFYVRFGHKLFQTEPDA
                     SHMGTREAPKLYSSFPAVSLSDVQLATFKVSNYFDIELGGKDEIEDLEEGDIPLVSTS
                     EFMNGVTAWRQPNVIYPPPAITVATDGSTCSSFVQEFPFYAFYKVAILRPKAQSYIPV
                     DALYFVAYLLKRERWRYVYARKFGKARIRQTILFAPSKDGEPDFKKMAELTRQCVSFP
                     LIESFRQAYGKAKEKSKHNRDSINHQHPKN"
     gene            1252..2079
                     /locus_tag="AC16GM_00320"
     CDS             1252..2079
                     /locus_tag="AC16GM_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922156.1"
                     /translation="MKHIGSVLSVIGVVIGLIGITLAVHYHSQSIQERVPTFYVEPVR
                     AKIFDATGQAPSELQVLHRGRAISERSVTAVQIYFWNEGELPIRKADILEPLAIELWP
                     PAEILAAHLVKQSRKVINFSLGAVPESTSNRLPLSFDILEKSDGAAIQIIFAGEPDAP
                     VALKGTIVGVGAPKLLSLSKGGGERPNPLVTFKLTQLMRNSLLAIGVVALLLIGALWL
                     AVRRRGQKSVMKFGIFVIVTAIFFGIFWGMYMAYDAEKTSSTGVPSGIWLTGESFKP"
     gene            2099..2602
                     /locus_tag="AC16GM_00325"
     CDS             2099..2602
                     /locus_tag="AC16GM_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2922157.1"
                     /translation="MDLDRIRALYDYNRWANAKILGAAAPLAPEAFTKDLGSSYRSVR
                     DTLVHILGAEWIWLERWKGTSPRALLPFEDFPTVDSIRKRWQEIERGQEQFLSGATEE
                     KLLASLSYINIKGLPFTYPLWQALQHVVNHSTYHRGQVITMLRQLSAQPVATDFLAFY
                     DERFGKR"
     gene            2620..3903
                     /gene="clpX"
                     /locus_tag="AC16GM_00330"
     CDS             2620..3903
                     /gene="clpX"
                     /locus_tag="AC16GM_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR2922158.1"
                     /translation="MNQQRPKNSNHTSDSTREGKTCSFCGRPAEEAGVLLSSPQAAIC
                     EECLLRGRELLSTQKTKKVAEEKPPDLPKPAEIKAFLDQYIIGQERAKKTVSVAVYNH
                     YQRVFHPEATAEVELEKANILLLGPTGTGKTLMAQTLAKMLHVPFSIADATVLTEAGY
                     VGEDVENILVRLLQSADYNLTRAQRGIIYIDEIDKIARKEANPSITRDVSGEGVQQGL
                     LKILEGTISNVPPKGGRKHPEQPFIQVDTKNILFVCGGAFDGLEKIIARRVGQKVVGF
                     AASPLQRGQGKIGEILAQVEPEDLLQYGLIPELIGRLPVVCAMDELDEAALYDILVKP
                     KNALVKQYRKLFELEGIDLEFTEEALREAVAQAQKRKTGARALRSILEEAMLDIMYEL
                     PSKPEVARCAITREVIAKTGAPEYLYRKEAAKKSA"
     gene            3958..4401
                     /locus_tag="AC16GM_00335"
     CDS             3958..4401
                     /locus_tag="AC16GM_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2922159.1"
                     /translation="MAGTLRRSADWAPVFLRLALAIVFIMAGSGKLFGWFGAPGFKGV
                     SGYIGMLGFKPAAFWTALLAVTEFGGGLAMLFGILTRWAGLGLAIVMAVALVRVHIKW
                     GLAVNGGDGHFDLPLVLLLVALAMTVTGAGKWSLEAKMKKAPAMT"
     gene            4470..5135
                     /gene="yihA"
                     /locus_tag="AC16GM_00340"
     CDS             4470..5135
                     /gene="yihA"
                     /locus_tag="AC16GM_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR2922160.1"
                     /translation="MLQITKAEFAKTVFEPGEAPRDQRPQIAFAGRSNVGKSSLLNIL
                     CRRKNLAQVSKTPGKTKALNFFLVNEQIWFTDLPGYGYAKVEKKLREQWGTLVEKYLQ
                     ETRDLARVIQIVDSRHPPFPDDLELAEWLSHIGLRFLVVLTKADKLSRGEEAQALKRA
                     QEAFSPSAPEKITLFSATSGRGRDEVLRWIGQVIRDYRDAEREVRHSRAGRGDEEMTE
                     HHA"
     gene            5128..6408
                     /locus_tag="AC16GM_00345"
     CDS             5128..6408
                     /locus_tag="AC16GM_00345"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR2922161.1"
                     /translation="MPNRIVVGMQWGDEGKGKVVDLLSREADIIARYQGGANAGHTVV
                     IGSEKFILHLIPSGILHPGKICVIGNGVVVDLEQLVTEIDELEKRQIGVSGRLFISGA
                     AHLVLPYHKALEQSQESHQREKIGTTGRGIGPAYTDKFARLGIRVIDLWDEKRLAAKF
                     TENLAWKRPLIEATNGKFDWNEKKLVDLACGYRDRIKPLVTDTSLYLHNAIKAGKNVL
                     FEGAQGTLLDVDFGTYPFATSSNTIAGGAMTGLGVGPRCVDEIIGVMKAYTTRVGNGP
                     FPTELTDAVGQKLQKRGNEFGATTGRPRRCGWLDILALKYAVRVNGITKIALMKLDVL
                     DGLPELKICIAYKFHGEQLKELPVDLAALGDCEPVYETYPGWSGVTSGVTVYGKLPEP
                     ARRYVEKIQKLLGVPMLLVSTSDRREETIWLQAS"
     gene            6456..6527
                     /locus_tag="AC16GM_00350"
     tRNA            6456..6527
                     /locus_tag="AC16GM_00350"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6488..6490,aa:Lys,seq:ttt)
     gene            6576..6648
                     /locus_tag="AC16GM_00355"
     tRNA            6576..6648
                     /locus_tag="AC16GM_00355"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6609..6611,aa:Lys,seq:ctt)
     gene            6766..7129
                     /gene="ssrA"
                     /locus_tag="AC16GM_00360"
     tmRNA           6766..7129
                     /gene="ssrA"
                     /locus_tag="AC16GM_00360"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            7362..7637
                     /locus_tag="AC16GM_00365"
     CDS             7362..7637
                     /locus_tag="AC16GM_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922162.1"
                     /translation="MMQNWLKIFGGFFWFGLGLIVLGKHATQYPEIRNFAGGGPLVAC
                     YVLTIFGTIFFVIGLYGVLSILRRFATENKLALRFFNLILPFGKFGL"
     gene            complement(7706..7933)
                     /locus_tag="AC16GM_00370"
     CDS             complement(7706..7933)
                     /locus_tag="AC16GM_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2922163.1"
                     /translation="MARGKVKWFNEKKGFGFIQQDGGGQDIFVHYTAIKAEGFKSLVE
                     GDEVEFTVQEGPKGPQATNVSRVVAEGNRAL"
     gene            8328..>9001
                     /locus_tag="AC16GM_00375"
     CDS             8328..>9001
                     /locus_tag="AC16GM_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR2922164.1"
                     /translation="MFSGLLDKRHFGRGQRLFPVVGLLLIWVAGVQAQPFLRSVTSFP
                     INDSAGPVAMPFLGGIDSPKPQLVDLDRDGDFDLVILQKDGRLVFFRNTGTLSSPQFD
                     YGADSFLGINAGQWFRLADIDGDGDPDLFVDNNTGGMRFYRNDSPPDSFIFNKISQNF
                     QNIFSGFNNTPAFADIDGDGDFDFFLGELTGTLTYWRNSGTPLSPNFVFGANDFDSIV
                     AFPTVG"
BASE COUNT         2058 a         2312 c         2572 g         2059 t
ORIGIN      
        1 attagcaagt attgtcccct actccctgct tgtgcgcact ggtgaatggc acaagaagat
       61 tttgaagaac aacaccttac tttttattgc gacccttccc cccgatttgt ttaatcccta
      121 ctcttcgttt aatactgcta tagttgtatt tcagaaagga attccccact tgagtaaacc
      181 agtctttttt tgtaaggtgt cgaacgacgg gttcaaacta aagaaaaaca ctcgggtctc
      241 acgtcctggc acacaattgg aacagatact caaagcttat tccacgaaac atgacatccc
      301 cgaagtctgc gctttccgac ctatttcctc tgaatccgag gagtggtcac cggaggctta
      361 tatcgagaac gctccgcact ctgatgaaga atttgtcaaa ggttttgaag acagtgtaaa
      421 aaatcaagcc tcattttatg tgcgctttgg acataaactt ttccaaactg agccggacgc
      481 gtcccacatg ggaacgcgag aagcccctaa actctattca tcttttcccg ccgtttccct
      541 ttctgacgtt caacttgcga cctttaaagt gtcgaactac tttgatattg aacttggagg
      601 caaagatgaa atagaagacc ttgaagaggg tgacattccg cttgtgtcaa ctagcgagtt
      661 catgaatggt gttacggcat ggcgacaacc gaatgttatt tatccccccc ctgctataac
      721 ggtggcaacc gacggaagca catgctccag ttttgtccaa gaatttccat tttatgcatt
      781 ttacaaagtt gccattttgc gtccgaaagc acagtcatat ataccggtag atgcacttta
      841 cttcgttgca tatcttctaa agcgagaacg ttggagatat gtttatgcac gcaagttcgg
      901 taaggcgaga ataaggcaaa cgattctttt tgctccttca aaagatggtg aacctgattt
      961 taagaaaatg gcagagctta ctcgccaatg cgtttccttt cctctcatag aatcattcag
     1021 acaagcgtat gggaaggcga aagaaaaaag taagcacaat cgagactcta taaaccacca
     1081 gcacccgaag aattagagcc gtattcgtta gattaacatc gaacggaaac gaagaagagc
     1141 atggatttag atcgtatacc ggcattgtac gactacagcc gctgggctaa tgccaatccg
     1201 cctcaggcgg actgggcgcg ggtgtaaccc gctggaggta acgacgtttt gatgaagcat
     1261 atcgggtcgg ttctatcggt tatcggggtc gtcatcggcc ttatcggaat taccctggcc
     1321 gtgcattacc atagtcaatc catccaggag cgcgtgccca cgttttacgt cgagccggtc
     1381 cgggccaaga tcttcgatgc cacggggcag gcgccctccg aacttcaggt tttgcaccgg
     1441 ggccgcgcaa tcagcgaaag aagcgtaacg gcggttcaga tttatttttg gaacgaaggg
     1501 gagctgccca tcagaaaggc cgatattctc gagccgttgg cgattgaact ctggccgccg
     1561 gcggaaattt tggcggccca cctcgtcaaa cagagccgaa aggtgatcaa cttttcgctg
     1621 ggcgcggtgc cggaaagtac cagcaaccgt ctgccgctct ccttcgatat actggagaaa
     1681 tcggacggag ccgcaatcca gatcatcttt gcgggtgaac cggacgcccc ggttgcctta
     1741 aaagggacca ttgtcggcgt gggtgcgccc aagctgctca gtctgtccaa gggaggcggc
     1801 gagcgcccca atcctctggt tacgtttaag ttgacacaac tgatgcgaaa ttcgctcctg
     1861 gccatcggcg tagtcgcctt gctcttgatt ggcgcgctct ggctggccgt tcggcggaga
     1921 gggcaaaagt cggtcatgaa attcggaatt tttgtgattg ttaccgccat tttctttgga
     1981 attttttggg gaatgtatat ggcctacgat gcggaaaaga cgtcgtccac gggagtgccc
     2041 tccgggatat ggctaaccgg cgagtcattc aagccgtaat cataaggcag aaaaaaacat
     2101 ggatttagac cgcatacggg cattgtacga ttataatcgg tgggccaatg ccaagatttt
     2161 aggcgcggcg gcgccgcttg cgccggaggc gttcaccaaa gacttgggga gcagttaccg
     2221 ctcggtgcgg gatacgctcg tccacatttt aggggcggag tggatctggt tagagcgctg
     2281 gaagggtact tcgccccggg cgcttttgcc attcgaagat ttcccgaccg tcgatagcat
     2341 acgaaaacgg tggcaggaga ttgagcgcgg acaagagcag tttctctccg gcgcgacgga
     2401 ggagaagctt ttggcatccc tttcctacat taacatcaag gggctaccgt ttacgtatcc
     2461 cctctggcag gcgctgcaac acgtggtgaa ccactccacc taccaccggg ggcaggtgat
     2521 tacgatgctc cggcagctga gcgcccagcc ggtggcgact gattttttgg cgttctacga
     2581 cgaacggttc ggcaagcgat aaccagagat ggagaaggca tgaatcaaca gcgtcccaag
     2641 aacagtaacc acacatccga ttccactcgt gaagggaaaa cctgctcctt ttgcgggcgg
     2701 ccggcggaag aagcgggggt tctgctctcc agcccgcagg cggctatttg cgaagaatgt
     2761 ctcttgcgcg ggcgggaact gctcagcact cagaagacca agaaagttgc ggaggaaaag
     2821 ccgcccgacc tgcccaagcc ggcggagatc aaggcctttt tagaccagta catcatcggc
     2881 caggagcggg cgaaaaagac ggtctcggtg gcggtctaca accactacca acgggtgttt
     2941 catccggagg caacagccga agtcgaatta gaaaaggcca acattcttct cctcggcccc
     3001 accggcacgg gcaagacttt gatggctcag acgctcgcca agatgctcca cgtccccttt
     3061 tccattgccg atgccacggt tttgaccgag gcggggtacg tgggcgagga cgtcgaaaac
     3121 atcttagtac gtctcttgca gtcggcggat tacaacctga cccgcgccca gcggggcatt
     3181 atctacatcg acgaaatcga caagatcgcc cgcaaggaag cgaatccctc cataacccgg
     3241 gacgtttcgg gggagggggt acagcagggg ctgctcaaaa tcttggaagg cacgatttcc
     3301 aacgtgccgc ccaaaggagg gcgcaagcac ccggagcagc cgttcattca ggtcgatacc
     3361 aaaaacattc ttttcgtctg cggcggggcg ttcgacgggc tggaaaagat catagcgcgt
     3421 cgtgtgggac agaaggtcgt ggggtttgcc gccagtccgt tgcagcgggg acaggggaaa
     3481 atcggcgaga ttctggccca ggtcgaaccg gaagacctgc tgcagtacgg attgattccc
     3541 gaattgatcg gccggctgcc ggtggtctgt gccatggatg agttggatga agcggccctg
     3601 tatgatattt tagtcaagcc gaagaacgcg ctcgtcaagc agtaccggaa gctgttcgag
     3661 ctggaaggga tcgacctcga attcacggaa gaggcgctgc gggaggcggt ggcccaggcg
     3721 cagaagcgga aaaccggtgc ccgcgccctg cgctcgatct tagaagaggc gatgctcgac
     3781 atcatgtacg agttgccgtc caaaccggag gtcgcgcgat gtgcgatcac ccgggaggtg
     3841 attgccaaga cgggagcgcc ggagtacctt taccggaagg aagcggcgaa aaagagcgcg
     3901 tagtcgagcg aaaaagatgc taggcggagg cgttcaccat caaccagagg agggaagatg
     3961 gcgggtacat tgagacggag tgctgactgg gcgccggtct tcttgcggct ggctttggcg
     4021 attgtgttca tcatggctgg ctcggggaaa cttttcggct ggttcggcgc ccccggtttc
     4081 aaaggtgttt ccgggtatat cggcatgctg gggttcaagc cggcggcctt ctggacggcg
     4141 cttctggcgg tcaccgaatt cggcggcggg ctggcgatgc tctttggcat cctcacccgt
     4201 tgggccggcc tgggactggc gattgttatg gcggtggcct tggtgcgggt gcacatcaag
     4261 tggggtctgg cggtcaacgg cggggacggc cactttgacc tcccgctcgt ccttttgctt
     4321 gtggccctgg cgatgacggt taccggtgcc gggaagtggt ctttggaagc caagatgaaa
     4381 aaagccccgg caatgacctg agctcgcttg aggaagtcat ttgacttaag ccgggcgggt
     4441 gaaaaacccg cccgttgtgt gtcggttata tgcttcaaat caccaaggcc gaatttgcca
     4501 agaccgtttt tgaaccgggc gaagcgcccc gggatcagcg ccctcagatt gctttcgccg
     4561 ggcggtcgaa tgtagggaag tcgtcacttt tgaatatcct gtgccggcgg aaaaatttgg
     4621 cccaggtttc caaaactccg ggcaagacga aggcgctcaa tttctttctg gtcaatgagc
     4681 agatctggtt caccgatctt cccggctacg gctacgccaa agtggaaaag aagcttcggg
     4741 agcagtgggg gactttggtt gagaagtatc tccaggaaac cagagacttg gccagggtca
     4801 ttcagattgt ggacagtcgc catccgcctt tcccggatga ccttgagtta gcggagtggc
     4861 tctcgcatat tggtctgcgg tttctggtgg tcttgaccaa ggcggataaa ctgtcccgcg
     4921 gcgaagaggc gcaggccctc aagcgggcgc aggaggcttt ctccccctcg gcgccggaga
     4981 aaatcactct gttttcggcg acgagtggtc gcgggcggga tgaggttttg cggtggattg
     5041 gacaggttat ccgggattac cgagatgcag aaagagaagt tcggcactcg cgagcgggtc
     5101 gtggagatga agaaatgacg gagcatcatg cctaatcgca tcgtcgtcgg tatgcagtgg
     5161 ggggatgaag ggaaggggaa ggtggtcgat ctcctttccc gcgaggccga catcattgcc
     5221 cgctaccagg gaggggccaa cgccggccat acggtcgtca tcggctccga aaaattcatt
     5281 ctccatttga tcccttccgg cattctccac cccgggaaga tctgcgtcat cggcaacggc
     5341 gtggtggttg atttggagca gctggtgacc gaaatcgacg agttagagaa gcgccagatc
     5401 ggcgtctcgg gacgactatt catcagcggg gcggcgcact tggtcctccc gtatcacaag
     5461 gcgttggagc agagtcagga gtcccaccag agagaaaaga ttggcaccac cgggcgcggc
     5521 atcggtccgg cctacaccga caagttcgcc cgcctgggca tccgggtgat tgacctctgg
     5581 gatgaaaagc gtttggccgc aaaattcacc gaaaatcttg cgtggaaacg tccactgata
     5641 gaggcgacca atggcaaatt cgattggaat gaaaagaaac tggttgatct ggcctgcggc
     5701 tatcgcgacc ggatcaagcc cttagtcacc gacacgtcgc tctacctcca caacgccatc
     5761 aaagcgggaa aaaacgtgct ctttgaaggg gcgcagggga cgcttttgga tgtcgacttc
     5821 ggcacctacc ctttcgccac gtcttcgaat acgatcgctg gcggggcgat gaccggcctc
     5881 ggtgtcggcc ccaggtgcgt ggatgaaata atcggcgtga tgaaggccta caccacccgg
     5941 gtggggaacg ggcctttccc cacggagttg accgacgcag tcggtcagaa attgcagaag
     6001 cgcgggaacg aattcggcgc caccaccggc cggccccggc gctgcggctg gctcgatatc
     6061 ttagcgctca aatatgcagt gcgggtcaac ggcatcacca aaatcgcctt gatgaaactg
     6121 gacgtgctgg atggattgcc ggaactcaaa atttgcatcg cctacaagtt ccacggcgag
     6181 caactgaaag agctgccggt cgatctggcg gccctagggg actgtgagcc agtctacgag
     6241 acttatccgg gctggagcgg cgttaccagc ggagtgacgg tctacgggaa gttgcccgag
     6301 ccggcccgac ggtacgtaga aaagattcaa aagttgttgg gtgtgccgat gttattggtt
     6361 tccaccagcg accggcggga ggagacgatc tggcttcagg cgtcgtagat gttctttgac
     6421 gaccattttc ggtctcgtat attcggatga aatccggggg tgtagctcag tggtagagca
     6481 tctgcctttt aagcaggagg ccggtggttc gatcccaccc accctcagta aaaacaggga
     6541 tcagtgatta gggattagtg atatataacc attgggcgcc tttagctcag ctggtagagc
     6601 aactgactct taatcagtgg gtcgcaggtt cgaatcctgc aaggcgcatt tatgaggttg
     6661 cgtggggagg cgacacagaa gaacttatcg gggaaggcgt tttcccgtat attaagaatg
     6721 agagttctgg ttctttctgg actccgagcg aagagtcgct ggattggggg cgacgggatt
     6781 cgacggagat aaggagggag tgccagcctg ccgaggtgcc cgggtgacct cgtaaaaccc
     6841 ctgggacatg tacaagtgcc aacgcgaacg cgtatgcact ggctgcgtaa ctaaaaccta
     6901 cgcaacccgt caactccgga tctgcccttc ggtcaggaac ttggcgtcga gccagaaggg
     6961 atagccccgg aaggttcccg aacttccggc gcgaaactca atagggatag cctgcggcca
     7021 agcctgtctg gcggcgttcc cacaggcgaa agcaaaagcc ggactgtgca gggagagagc
     7081 gctcctgaat ttctcacgga cgtgggttca actcccaccg cctccattta tttcgctcca
     7141 ctatgatcga acaatctcaa cgcagtgaga tgccgcgact tctttcggtt ctgcaatcgg
     7201 tttcccatta ccatcttcta taaaatccgg acaattaagc attcagcgtg gtaattgggt
     7261 taacccgtag tcatttaagt ggttccggga gtccttaatg ttcttgtaga cgggcattgg
     7321 gcttgcgttt agaggataca gagggaaagg agagaggtaa aatgatgcaa aactggctga
     7381 aaatcttcgg tgggtttttt tggtttggcc taggattgat cgtcctcggg aaacatgcaa
     7441 cacagtaccc cgaaatccgg aactttgccg gcggcggacc gctggtcgcc tgctatgtgc
     7501 tcaccatctt cgggacgata ttttttgtga ttgggttgta cggggtgctt tccattttgc
     7561 gccgatttgc cactgaaaac aaattggcgc tccgattctt caatctgatt ttaccttttg
     7621 gcaagttcgg cttgtgaccg gcgggaaaaa gcgaaaaggc caggaatatt cctggccttt
     7681 tcgcttttcg gtagaaagct aaactttaca gtgcccggtt accctccgca accactctgc
     7741 tcacattcgt tgcctgtggt cctttaggac cttcctgaac tgtgaattct acctcatccc
     7801 cctcaaccaa ggatttgaat ccctctgcct ttatggcagt gtagtggaca aaaatgtcct
     7861 gtcctccacc atcctgctgg atgaagccaa atcccttttt ttcattgaac cacttgacct
     7921 tacctctggc caatcgaatt cacctcctcg gattttcgcg tatgatacga tcatttctgc
     7981 ttaaggcgat gcccatccgc cacttaacct tttacctcgc aaattggtta cactggtagc
     8041 acaagttctt attgatggga agtggaaaaa caaaagtggg cccgcccaaa cgcatgcgca
     8101 aaatctaacc ttattcagta atttacaata gtagtgagaa tttgtcaagt attttttccg
     8161 aagacatgaa aatttttttg acagggttta cccgatcgaa aatactggta gcatcaggca
     8221 gagtgaaagc gtagttttaa tagcaatatg gagaccacaa ggattcgtct cggacggatg
     8281 ggtaagtcga aagcaactcg tgttcatcaa tctgactcaa ataacgggtg ttttccggtc
     8341 tattagataa aaggcatttt ggtcggggcc aacggctttt tcccgtggtc ggtttgcttc
     8401 tgatctgggt ggcaggcgtc caggcccaac ctttcctgcg ttcggtcacc agtttcccga
     8461 taaacgacag tgccgggccg gtggccatgc cgtttttggg gggtattgac agccccaagc
     8521 cccaattagt cgacctcgac cgggatggtg attttgacct ggtcatcttg cagaaagacg
     8581 ggcggctggt cttcttccgg aacaccggca ccctcagttc gccgcaattt gattacgggg
     8641 ctgattcttt tttggggata aacgccgggc agtggttccg cttggccgac atcgacggcg
     8701 acggcgatcc cgacctgttc gtggacaaca acaccggcgg catgcgcttc taccgcaatg
     8761 acagcccgcc ggacagtttc attttcaata aaatcagtca gaactttcag aacatcttct
     8821 ccggcttcaa taacaccccc gcgtttgccg acatcgacgg tgacggcgat ttcgacttct
     8881 ttctggggga attgacgggc accctgacgt actggcggaa cagcggcacc ccgctttcgc
     8941 ccaatttcgt cttcggcgcg aacgatttcg attccatcgt cgcctttccg accgtcgggt
     9001 g
//
LOCUS       DBNGZD010000013         2440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1668527, whole genome shotgun sequence.
ACCESSION   DBNGZD010000013 DBNGZD010000000
VERSION     DBNGZD010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1668527"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="AC16GM_00380"
     CDS             complement(<1..1042)
                     /locus_tag="AC16GM_00380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR2922165.1"
                     /translation="MPRILIDNKYEVVRKLREGGFGVIFYGWDLTLDKPVAIKEIAPS
                     LLGHQQYLDMFTDEALNTAKLSHANIVSVYDLRRTPEGRIFIIMEYLDGVDLRTALER
                     CRTDGVPFPVDLAVQVIAEVCKALEYAHTARDRRTGLPLNIIHRDISPSNIMITATGE
                     VKLIDFGVAKARTRVARKTRTGILKGKVSYMSPEQLEGKVIDRRSDIFSLGVVLYETL
                     AGEKLFKGDSDYSVMKEIVAARIDLQPLTLRQIPPRLQAIVQKALDKDLFRRYQSAGE
                     MQIDLTTYQKTAGTILPQVELARWCANYLVGHKVEEKKESVSAVLKRVSEDSEQVIAT
                     KEVTCSEQEYDEE"
     gene            1398..>2440
                     /locus_tag="AC16GM_00385"
     CDS             1398..>2440
                     /locus_tag="AC16GM_00385"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR2922166.1"
                     /translation="MRYLRLASAITAFLFFALIGAAALAAQSAHPQKVEALRVAEPIK
                     IDGILSESVWQRPATSNFTQRDPTEGVSPTESTHVWVAYDNVAVYVAARLYDRTPDSI
                     TARLGRRDAGMSSDLFAFFVDPYYDRRSGFYFGLNPAGTFYDGVLYNDDWDDDSWDGV
                     WEGKAKIDSLGWTAEMRIPYSQLRFHKKDQYLWGVNFERDIARKNERDFLVFTPKNGS
                     GFVSRFVDLVGIENIEIPYHLEVLPYLTTKAEYTRHAPGDPFNDGSKFLPGTGADLKV
                     GLGSDLTLAATVNPDFGQVEVDPAVVNLSDVETFFQEKRPFFIEGSSIFNFGQGGSNS
                     YWNFNWPGPEFFYT"
BASE COUNT          496 a          745 c          661 g          538 t
ORIGIN      
        1 cttcctcatc atactcctgc tccgagcagg tcacttcctt ggtggcaatc acctgctccg
       61 aatcttccga gacccgcttt aagaccgcgc tgacgctctc ttttttctct tcgaccttat
      121 gtcccaccag gtaattcgcg caccaccggg ccaactccac ctgggggaga atcgtcccgg
      181 cggttttctg gtaggtggtg agatcaatct gcatctctcc cgcgctctgg tagcgacgga
      241 agagatcctt gtccagcgcc ttctgcacaa ttgcttgcag ccggggggga atctggcgca
      301 aggtgagggg ctggagatcg atccgagccg ccacgatctc cttcataacg gagtaatcgc
      361 tatccccttt gaacagtttc tcgcccgcca gggtctcgta cagcaccacg cccaaagaga
      421 agatgtccga gcgccggtcg atcaccttcc cttctaattg ctccggcgac atgtagctca
      481 ctttgccttt caggatgccg gtgcgggtct tcctggcgac ccgggtgcgg gccttggcca
      541 cgccgaagtc gatcaacttc acttcgccgg tggcggtaat catgatgttc gaaggcgaaa
      601 tgtcgcggtg gatgatgttc aaaggcaggc cggtccggcg gtcgcgggcc gtgtgcgcat
      661 actccagcgc cttgcaaact tcggcgatta cctggaccgc taaatcgacc gggaacggca
      721 ccccgtcggt gcggcagcgc tccagggccg tgcgcaggtc aacgccgtct aaatactcca
      781 tgatgatgaa aatccgcccc tccggcgtgc gccggaggtc gtagaccgaa acgatattgg
      841 cgtgggacaa cttggcggtg ttcagcgcct cgtcggtgaa catgtccaag tactgctggt
      901 ggcccagaag cgacggcgcg atttctttga tcgccaccgg cttgtccaga gtcaaatccc
      961 agccgtagaa aatgaccccg aacccgccct cccgcagttt gcggacgacc tcgtatttgt
     1021 tgtctatgag aatgcgcggc attactcgac tgagctcacg acgttgttat cagttgtagc
     1081 cgaggatttc atcccatacg ggtcgtaccg acctcggcgc cgaaggcgta gatttcactc
     1141 aaagcgcaaa aaatatctcc tccccttgcc ggccttttcc taaactatca ggcgggcaac
     1201 gggttagtcg ttggtagtta tatcggcaga aatgtcgtgg cctcgactct ccaagccgat
     1261 ttgcccggac cggaagagat tcccctcttc tttttgaaac cggaggccgc aaaagacgta
     1321 caagggacta aagatgcagc taaatgagat cgggtcggga aactcgtctt gaaactctga
     1381 gcccttcgca aaaggatatg cgttacctcc gactggcctc cgccatcact gcattccttt
     1441 ttttcgccct gattggagcc gccgcgctcg ctgcccagtc ggcccacccg caaaaagttg
     1501 aagccctgcg tgtcgccgaa ccgatcaaaa ttgacggcat cctttccgag tcggtctggc
     1561 aaaggcccgc aacgtccaat tttacccagc gagaccccac cgagggtgtg tcgccaaccg
     1621 aaagcacgca cgtgtgggtg gcgtatgata acgtggccgt ttacgtcgcc gcccggctgt
     1681 acgatcggac gcccgattcc atcaccgccc ggctgggccg gcgcgatgcc ggtatgagtt
     1741 ccgacctctt cgcatttttc gtggacccgt actacgaccg gcgttcgggc ttttactttg
     1801 gcttgaatcc ggccggaacg ttctacgacg gcgtcctcta caacgacgac tgggacgacg
     1861 attcctggga cggcgtctgg gagggtaaag cgaaaatcga ctcgctgggc tggaccgccg
     1921 aaatgcggat cccttattcc cagcttcgct tccacaagaa agaccaatac ctctggggcg
     1981 ttaattttga acgggatatc gcgcgaaaaa atgaaagaga ttttctggtt tttaccccca
     2041 aaaacggcag cggtttcgtt tcccgcttcg ttgatctcgt cggtatcgag aatattgaaa
     2101 tcccctacca cctcgaggta ctgccctacc tgaccaccaa agcggagtac acccgccacg
     2161 ctcccggcga cccgttcaac gacggctcca aattcctgcc cggaacgggc gccgacctga
     2221 aggtggggct gggcagcgac ctgactctgg ccgccacggt caatcccgat ttcggacagg
     2281 tggaagtcga tcccgccgtg gtcaacttga gcgacgtgga gaccttcttc caggagaaac
     2341 gccccttctt tatcgaaggc tccagtattt tcaacttcgg tcagggaggg tccaatagct
     2401 actggaactt caactggccg ggccccgaat ttttttacac
//
LOCUS       DBNGZD010000014        10969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1182661, whole genome shotgun sequence.
ACCESSION   DBNGZD010000014 DBNGZD010000000
VERSION     DBNGZD010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1182661"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC16GM_00390"
     CDS             <1..201
                     /locus_tag="AC16GM_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HIR2922167.1"
                     /translation="AAFIFSYLIGFLALFVPGGLGVREGLLTLSLSAYLPAGVATAVA
                     IGSRLWMTAIELLALLGIRRAR"
     gene            301..1014
                     /locus_tag="AC16GM_00395"
     CDS             301..1014
                     /locus_tag="AC16GM_00395"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR2922168.1"
                     /translation="MRALVAIPTYNEKENLEKIVPVVLAQDVCLEVLVVDDNSPDGTG
                     QIADRLAQANPRVHVLHREKKSGLGQAYLAAFRWALEHGYDLVFEMDADFSHDPKYLK
                     DFLVAIKEADLVIGSRYISGVNVINWPMGRLLLSYYANVYTRLVTGLPLRDATGGFKC
                     YRRKVLETIDLAAVRSNGYSFQIEVSFRAWKKGFRLREIPIIFIDRQAGTSKMSKKIV
                     REAVWIVWRLRLWSLLGRV"
     gene            1036..1995
                     /locus_tag="AC16GM_00400"
     CDS             1036..1995
                     /locus_tag="AC16GM_00400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2922169.1"
                     /translation="MAMATDLSIIIVNYNTADCLVHCLSALFGSPPAMAWEVLVVDNA
                     SSDRSVPCLRSDFPQVRLLVNNRNLGFGAAGNRGFAKAKGEFILFMNADVIVCPQAID
                     QLVRTLRSRPEVGVCGGKLVSPDGTVQPTFRRFPTHRSILFARGSLLGKIFPDRRGRY
                     VLPEVDDTVAVDSVAAAFLLTRRSALEQMQGFDERFFLYAEDLDFCRRMKDAGYEVLY
                     VPRALATHQWGASTRQNRSLAWRAHHRSIYRYFRKHYPQKKVSNFFLYLLLQAQLFLL
                     LALNFWRREEAAEGNPVSKAAALPAGRTQSEATAAVSATGLSC"
     gene            1989..3065
                     /locus_tag="AC16GM_00405"
     CDS             1989..3065
                     /locus_tag="AC16GM_00405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2922170.1"
                     /translation="MLRYALVFAVAGLTTWFLTPLVLRLCWRYGWFDHPNGRKVHQNP
                     VPRLGGVAIFFGFWLAVAVGLARSPDRFYLATEQFAPIFFGSLVILAIGLYDDLKGAV
                     PAAKLLAQITAGLILIAWGIQIRVLYIPFVGGVALGATSVPVTLLWLLALANTINLID
                     GLDGLAAGISAIVAASLLLTGLFLKIESVAIISAAVMGATLAFLRFNWHPARLFMGDS
                     GSLVLGFLFASVSIICPVKSITGVALFVPLLALGVPLIETASAIVRRISAKQKLLAAD
                     NQHLFHYLLGLGLSQRAVVWLFYVAACACLFVVLCLLTLDRRVIVSALMVLYVMLFLA
                     LAFALRQLTGRSEKGVLNAPPEKV"
     gene            3067..4047
                     /locus_tag="AC16GM_00410"
     CDS             3067..4047
                     /locus_tag="AC16GM_00410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR2922171.1"
                     /translation="METQTYLDFEKPIAELEKKIKDIRDFATGEAVELDREIKGLQRR
                     LERLERDIYSNLSRWQRVQLSRHPRRPLALDYIVLITTEFIELHGDRGFADDRALVGG
                     FAKIDGRKVLMLGQQKGRDTKQKLYHNFGMMHPEGYRKALRLMLLAEKYRKPVVVLVD
                     TPGAFPGVGAEERGQAEAIARNLREMARLSVPIIVVIIGEGASGGALGVGVGDLILML
                     ENAWYSVISPEGCAAILWRDAEKKPLAAEALKLSAFDLLGLGIIDKIIPEPLGGAQRD
                     PESQAARVKTEILQGLEALETLAPEELVTRRLQKYREMGAYHELLPGENL"
     gene            4060..4236
                     /locus_tag="AC16GM_00415"
     CDS             4060..4236
                     /locus_tag="AC16GM_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR2922172.1"
                     /translation="MMLDEKFLSILACPKCKGELAYRPELQRLICPACRLQYPIQDDI
                     PILLVEKGEPLPQG"
     gene            4280..4741
                     /locus_tag="AC16GM_00420"
     CDS             4280..4741
                     /locus_tag="AC16GM_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR2922173.1"
                     /translation="MKLSKFCEEDLITFELKSETKDDVIRELVELAARSKMVRDKVEL
                     SRAVVEREKLVTTGVGYGVAFPHAKTKAVKGVVIAFGRSKEGIDFEAMDRKPVHLFFL
                     IAAPEDAIGAHLNVMARLSYIMRSEKNREKFGTIKSPGEFLEQLDLASEAT"
     gene            4772..5599
                     /gene="mreC"
                     /locus_tag="AC16GM_00425"
     CDS             4772..5599
                     /gene="mreC"
                     /locus_tag="AC16GM_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR2922174.1"
                     /translation="MRAFPVLIAKYRPTLLLITGLVISGGTAVLPVPVKFGVANFLVR
                     IVYAPFFRISNKIDELKSLYVANRDLNRVLAELSLENARLRTAAEENARLRELLGFAE
                     PLPYHLIPAEVITLDPQYPFRSALIAAGTERGVRRFLPVIDRFGVAGKISAVTARQAE
                     VQLLFDPGFRAAARDLRSGVLGIVRWKKEFLLAFDNVPNREDVQIGDSVITSGLGGVF
                     PEGLPLGRVIRVQTDSMAFFKSILVEPLARLATVDELFVLALPPATRSGALEGEPEP"
     gene            5596..6084
                     /gene="mreD"
                     /locus_tag="AC16GM_00430"
     CDS             5596..6084
                     /gene="mreD"
                     /locus_tag="AC16GM_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR2922175.1"
                     /translation="MKFLAPVVLLLGVVVYQSLFSELFSIAEIKPDLALALTVYLALA
                     RGPEAGLAFGFFSGLCLDVLSPLSLGTGMFIRALIGYLVGNFKDHLFLDTLSAKGMVV
                     AFASLVAGLFQAFWTSGPRVGHVLWFLWRYGFLIALYTGLLGIFIFAVAEGKVRLKPK
                     TT"
     gene            6088..7926
                     /gene="mrdA"
                     /locus_tag="AC16GM_00435"
     CDS             6088..7926
                     /gene="mrdA"
                     /locus_tag="AC16GM_00435"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR2922176.1"
                     /translation="MELSREQRGLLVRLILLVFGGVLALRFFFLQVVSYGTYYRISEE
                     NRLRLVPIPAPRGILYDRSGRVIVDNRTTYTVSVIPSEVKDIDRLAQTLAPLLGLDRE
                     AFWARFKRGPAKPNEPVRVKRDVDFRTIAVLEEQNEDFPGVLYQVEQTRNYPPDTLGS
                     HFSGYIGEASETELTKNPQLWRGTTIGKQGLERIYDDLLRGGDGAIFLEVTAQGKVLG
                     QLADRPPEPARPGSDLFLTIDLDIQAVAESALAGSCCGAAVALDPRTGEVLAFVSKPG
                     FDANAFVTGLSLDQWQKVSGDSTHPRLNRTIQGLYPPGSTVKLLTAGAALESHLITPA
                     KRFNACQGALWFGNRLFRCWKPEGHGSLDLYQAIVQSCDVYFYQLGLALGLERWGEYA
                     AKCGFGQKTGIDLTDEWAGVLPTAAYYARRFGQNQWPKGVMLNLSIGQGEFLVTPLQL
                     ATFFGALGNDGAVFRPHLLKRYVDPDGLEREIEPVCVRQLPFSPGTLRFLKTALVGVV
                     NDPHGTATVAQLPGISVAGKTGTAQNPHGLEHAWFACFAPAEKPEIAVAVLVENAGHG
                     GTVAAPIAREMLRAYFTKTHPLLQPEVAASAGVSDRSRRPATPGGD"
     gene            complement(7942..8370)
                     /locus_tag="AC16GM_00440"
     CDS             complement(7942..8370)
                     /locus_tag="AC16GM_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922177.1"
                     /translation="MNQTTTQPSLVRSVRAAGIVVLCALGATFSITQCSKKTIKASLF
                     IFRGTVTAKESGNLLRNVEILLNGNSRDTTDASGRYSFAEEIRTDRQLNAVPVFDSLP
                     DYTQCGTGRFSVPAGDTVWITNFGLCRRPPAPPCTCTVSR"
     gene            8434..9663
                     /gene="rodA"
                     /locus_tag="AC16GM_00445"
     CDS             8434..9663
                     /gene="rodA"
                     /locus_tag="AC16GM_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR2922178.1"
                     /translation="MRLSLIRSPEFDRWLTVDALFLSLLGVVLIYSATHTAIDPGEHQ
                     LPWKQLLWLPVAFFGFFAAFFLPLRLHEALAYVYYAAGAILLAAVLILGETRMGATRW
                     FAFGTFNLQPSELAKITAVFGLARLFAHAREPVTGLRRLVVAVVMILLPVGLILRQPD
                     LGTALVFCALGLAMLFWSGLPLWHLALLVSPLFSLLAAFHWITWALFFVLLLWVLYKT
                     RPGFLFGTICLVANLLFGMVTPLVWNRLHDYQKMRILIFLDPGRDPQGAGYQLIQAKV
                     AIGSGGIFGKGFLHGSQTKLAFLPAQHTDFIFTVVGEEFGLLGVLIVLALLGFLVYRG
                     LQAAIRCRNQFASYVALGLTSILAFQILVNVGMAAGLMPVTGLPLPFLSYGGTSLLTN
                     WVIIGLLANIAVHWQDY"
     gene            complement(9664..10221)
                     /locus_tag="AC16GM_00450"
     CDS             complement(9664..10221)
                     /locus_tag="AC16GM_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HIR2922179.1"
                     /translation="MTKTRILKSQTAVIFAILLAFWVTLPAGAVEITLDVTQRAAVVQ
                     SNRSQALVKFEIPTILRGARIDLALLEMRLFADTTEGTRVHFALHPAATTWLPSNQMV
                     VDPNVAVVDTVGVGVVHDFRQSNKVIVNITEIVQGWVDGTFSNLGFRLHSSLSPRCLF
                     SLARDASWPLNTFARVRIRFTPPER"
     gene            complement(10218..10667)
                     /locus_tag="AC16GM_00455"
     CDS             complement(10218..10667)
                     /locus_tag="AC16GM_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2922180.1"
                     /translation="MSTTTLKAEGNWWGTTTPDSTHNFMGPVDFRPFCDDSTCLPPEE
                     PMSKPLAGGVSLGFQFGLEQNEPNPFNPLTRIRFTLPEPGQVTLKVYNLIGQQVATLV
                     NEYRDAGSHEVIWNGKDREGKPVASGVYFYTVTAGEHRATRRMVVLK"
BASE COUNT         2031 a         3123 c         3298 g         2517 t
ORIGIN      
        1 gccgctttca ttttctccta cctcatcggt ttcctggcac tttttgtacc aggcggtttg
       61 ggagtgcggg aggggttgct caccctgtct ttgtccgcct acctccctgc cggggtggcc
      121 acagccgtag ccatcggctc gcggctctgg atgacagcca ttgagctcct ggcgcttttg
      181 ggaattcgaa gggcgcgttg accctctcga agcggccgtc tatattcatg caaacgtgat
      241 gtgctggagg aaaattgcac gtggactttc attcccctcc ccactaggcc cggagcgagc
      301 atgcgggctc tggtagccat acccacgtat aatgagaaag agaatctaga aaaaatcgtg
      361 ccggtggtct tagcgcagga tgtttgtcta gaggttctgg tggtggacga caactcgccg
      421 gacggcaccg gccagattgc tgaccggctg gcacaggcta acccacgggt gcacgtcctg
      481 caccgggaaa agaagtccgg cttggggcag gcctacctgg cggccttccg ctgggcgctc
      541 gagcacggct acgacctcgt gtttgaaatg gacgccgact tctcccatga ccccaaatac
      601 ctgaaagact ttttggttgc aatcaaagaa gctgatttag tgataggttc gcgttacatt
      661 tcgggggtga acgtcatcaa ctggcccatg gggcggttgc ttttgtcata ctatgccaat
      721 gtctacacgc ggctggttac cgggctgccg ctgcgggatg cgaccggcgg cttcaagtgc
      781 taccggcgca aggtgctgga gacaatcgat ttggctgcgg tacgctccaa cggctactcg
      841 ttccagatcg aagtgagttt ccgggcctgg aaaaaagggt ttcgtttgag agaaattccg
      901 attatcttta tcgaccgaca agccgggacg tccaaaatgt ccaagaaaat cgtgcgcgaa
      961 gcggtttgga tagtctggcg cttgcggctg tggagtcttt tgggtcgggt gtagtgcccc
     1021 ggctaccgac gcggtatggc aatggcgacc gatctttcca ttatcatcgt caattataac
     1081 acggcggact gtctcgtcca ttgtctctcc gccctcttcg ggtcgccgcc agccatggcg
     1141 tgggaagtgc tggtggtgga taatgcctct tccgaccgga gtgtcccctg cctccggagc
     1201 gatttccccc aggtacggtt gctggtaaac aaccgcaatc tcgggtttgg cgcggccggt
     1261 aaccggggtt ttgcaaaggc gaaaggcgag ttcattttat ttatgaatgc cgacgtcatc
     1321 gtttgtccgc aggccatcga ccagttggtg cgaacgctcc ggtcgcgtcc tgaagttggc
     1381 gtgtgcggcg gcaagctcgt ttcgcccgat ggaacggtac agccgacctt ccggcgcttc
     1441 ccgacccacc ggagcatcct ttttgcccgc ggctctcttt tgggaaaaat ctttcccgac
     1501 cggaggggcc gctacgtttt gccggaagtc gatgacacgg tcgccgtcga ttctgttgcc
     1561 gccgccttcc tgctcacccg acgttccgca ctcgagcaga tgcaaggttt tgacgaaagg
     1621 tttttcctct acgccgaaga tctcgatttc tgtagaagga tgaaggacgc tggctatgaa
     1681 gtgctctacg ttcctcgtgc tttggccact caccagtggg gtgccagcac ccggcaaaat
     1741 cgctcgctgg cttggcgcgc acatcaccgg agcatttacc gctatttccg aaagcattat
     1801 ccacagaaga aagtgtccaa cttttttctg tacctcctgc ttcaggcaca attattcttg
     1861 ttgctcgccc tcaatttctg gcgcagggag gaagcagcgg aagggaatcc ggtaagcaaa
     1921 gccgcagccc tccccgccgg tcgtacccag tctgaggcca cggcggcggt ctccgcaacg
     1981 ggtttgtcat gttgaggtat gcgctggtct ttgccgtagc ggggctgacg acctggtttt
     2041 tgacaccgct ggtcttgcgg ctctgctggc gctatggctg gtttgatcat cccaacggac
     2101 gcaaagtgca ccaaaatccg gttccgcgct tgggcggcgt ggctatcttc tttggtttct
     2161 ggttggccgt ggcggtggga ctcgcgcgtt ctcccgaccg gttctaccta gccaccgaac
     2221 agtttgcgcc catatttttc ggttctctgg tgattctggc cattggactt tacgacgacc
     2281 tcaagggagc agttcccgca gccaaactgt tggcccagat caccgccggt ttgatcttga
     2341 ttgcttgggg tattcaaatc cgtgtcctct acattccgtt tgtggggggt gtcgcgttgg
     2401 gagcaaccag cgtcccggtg actctgctct ggcttttggc gttggccaat accatcaatt
     2461 tgattgatgg actggacggc ttggcggccg gcatttcggc cattgtggcg gcgagtttgc
     2521 ttcttaccgg tctcttttta aaaatcgagt cggtggccat catctccgcg gctgtcatgg
     2581 gagccaccct ggcgttcctg cgcttcaact ggcatccggc caggctcttc atgggggact
     2641 ccggctctct agtcctgggt ttcctcttcg cttcggtttc gatcatctgt ccggtgaaaa
     2701 gtatcaccgg ggtggcgctt ttcgtaccgc ttttggcatt aggcgtgccc ttgatcgaaa
     2761 ccgcttcggc tatcgtccgc cggatctcgg ccaagcagaa acttttggcg gctgacaacc
     2821 agcacctctt tcactacctt ctgggattgg gcttgagcca gcgggcggtg gtctggctct
     2881 tttacgtggc cgcttgcgcg tgtctctttg tcgtcctttg ccttttgacc ttagaccggc
     2941 gggtcatcgt ttcggcactc atggttctct acgttatgct ttttctggct ttggcttttg
     3001 cgctgcggca actgacggga cgctcggaga aaggcgtcct caatgcccca ccggagaaag
     3061 tctaatatgg aaacccagac ctacctcgat ttcgaaaaac cgatcgccga gttagaaaaa
     3121 aaaatcaagg acatccgcga cttcgccacc ggcgaggcgg tggagctcga ccgggagatc
     3181 aaaggactcc agagacgcct ggaacggctg gagcgggata tctactccaa cctctcgcgc
     3241 tggcagcggg tgcagttgtc ccgccacccc cgccgacctc tggcgctgga ctacatcgta
     3301 ctcatcacga ccgagttcat tgaactgcac ggcgaccggg gattcgccga cgaccgggcg
     3361 ctggtgggcg ggttcgccaa aattgacgga cggaaggtgc tgatgttagg ccagcagaaa
     3421 gggcgcgaca ccaagcagaa actctaccat aacttcggga tgatgcaccc ggaagggtac
     3481 cgcaaggcgc tccggttgat gctcttggcc gaaaaatata gaaagccggt ggtggtgctg
     3541 gtcgatacgc cgggggcgtt ccccggcgtg ggcgccgaag aacggggcca ggccgaagcc
     3601 atcgcccgca acctacgcga aatggcgcgg ctctcggtcc cgatcatcgt ggtgatcatc
     3661 ggggaaggtg cttccggtgg tgccttgggt gtgggcgtgg gagatctcat cttgatgctg
     3721 gaaaatgcct ggtattcggt gatttccccg gaagggtgcg ctgccatttt atggcgcgat
     3781 gcggagaaaa aacctctggc ggccgaggcg ctcaagctgt ccgctttcga cctcctggga
     3841 ttggggatta tcgataaaat cattccggaa ccgctcggcg gagcgcagcg tgaccccgaa
     3901 agccaggccg cccgggtgaa aaccgaaatt ctccaagggc tggaagccct ggaaacactc
     3961 gccccggaag aactggtgac ccggcgactc cagaagtacc gggagatggg ggcctaccat
     4021 gaactgctgc ccggggaaaa cctctaaggg taaggacaaa tgatgttaga cgaaaaattt
     4081 ctgtccatct tagcttgccc gaaatgcaaa ggggagcttg cgtaccggcc ggagctacag
     4141 cgcttgattt gtccggcctg ccgcctgcaa tacccgatcc aggacgacat tcccattctg
     4201 ctcgtcgaaa aaggggagcc gctcccgcag gggtagattt ttctttccga acaaccgata
     4261 gtcgctacgg aggccaggga tgaaactttc caaattctgc gaagaagact tgatcacctt
     4321 cgaactgaag agcgaaacga aagacgacgt catccgcgaa ctggtggagc tggcggcccg
     4381 gtcaaaaatg gtgcgggaca aagtggagct ctcccgggcg gtggtggagc gggagaagct
     4441 ggtgactacc ggggtcggct acggcgtcgc cttcccccat gcgaagacca aagcggtcaa
     4501 gggggtggtc atcgcctttg ggcgctccaa ggaggggatc gattttgagg cgatggaccg
     4561 caagccggtg cacctcttct ttctcatcgc cgcgccggaa gacgccattg gcgcccacct
     4621 gaacgtcatg gcgcgactct cctacatcat gcgcagcgag aagaaccggg agaaattcgg
     4681 cacgatcaaa agcccgggtg aatttttgga acagctcgat ttggccagtg aggcgacgta
     4741 gccggggcac cgcggaaacc gcgttcgacg tatgcgcgcc ttccccgttc ttattgccaa
     4801 ataccggccg accctccttt tgattaccgg tctcgtcatt tccggcggca cggcggtgtt
     4861 gcccgtgccg gtcaagtttg gcgtggccaa cttcctcgtc cggatcgtct atgctccttt
     4921 ttttcgcatt tcaaacaaaa tcgatgagct gaagtccctc tacgtggcga accgtgacct
     4981 gaaccgggtt ttggccgaac tctcgctgga gaacgcccgg ttgcggaccg ccgcagagga
     5041 gaacgcccgt ctgcgggagc ttttggggtt tgccgagccg ctcccctacc atctgatccc
     5101 ggccgaagtc attacgctgg atccgcagta cccatttcgg tcggcgctca tcgccgccgg
     5161 aaccgaacgg ggggtgcggc ggtttttgcc cgtgatcgac cgcttcggtg tagccggcaa
     5221 aatcagcgcg gtgaccgccc gtcaggccga ggtgcaatta ctctttgacc ccgggttccg
     5281 ggcggcggcg cgcgacctgc ggtcgggcgt tttgggaatc gtccggtgga agaaggagtt
     5341 cctgctggcg ttcgataacg tgcccaaccg ggaagacgtg cagatcggcg attccgtcat
     5401 tacttccgga ctggggggcg tctttccgga ggggttgccg ctcggccgtg tcattcgggt
     5461 gcagaccgat agcatggcgt tcttcaagtc aattttggtg gaacctttgg cgcgcctggc
     5521 gacggtggat gagctgttcg ttctggcact gccgccggca acccgttcgg gcgctttgga
     5581 aggagaaccg gaaccgtgaa gttcctcgca cctgtggtgc tgcttttagg agtggtcgtg
     5641 taccaatcgc ttttttccga actgttcagc attgccgaga tcaagcccga tctggcgctg
     5701 gcactcacgg tgtacctggc gttggcccgg ggtcccgaag cggggctcgc cttcggcttt
     5761 ttctcaggac tttgcctgga tgtgttaagt ccgctctctc tgggcaccgg gatgttcatc
     5821 cgggctttga tcgggtatct ggtgggaaac ttcaaggacc atctcttcct ggacacgctc
     5881 tcggccaagg gaatggtggt ggcgttcgct tcgctcgtgg cgggactgtt ccaggcgttc
     5941 tggacgagcg gcccccgagt gggacatgtg ctctggttcc tctggcgcta tgggtttttg
     6001 atcgcgcttt acaccgggct tttgggaatt tttatttttg ccgtggccga aggcaaggtg
     6061 cgtttgaaac cgaaaaccac gtaagtcatg gaactttccc gagaacagcg gggtcttctg
     6121 gtccggctta tccttctggt ttttggcggg gtgctggccc tgcgtttttt ctttctgcag
     6181 gttgtctcct acggcaccta ctaccggata tcggaagaaa accgcctccg tctggtaccg
     6241 attccggcac cgcggggcat tctgtacgac cgctccggcc gggtaatcgt cgataaccgc
     6301 accacgtata ccgtctccgt gattccctcc gaggtcaaag atatagatcg gctggcgcag
     6361 acattggcac cgcttctggg tctggaccgg gaagcttttt gggcccggtt caaacgcggg
     6421 ccggcgaaac ccaacgagcc ggtgcgcgtg aagcgcgatg ttgattttcg gaccattgcc
     6481 gtgctcgaag aacaaaacga agactttccg ggagtcctgt accaggtgga acagacgcgg
     6541 aactatcctc ccgacacctt gggcagccac ttctccggtt acattgggga agcctccgaa
     6601 acggaactga ccaaaaatcc gcaactgtgg cggggcacca cgattgggaa acagggactg
     6661 gaaagaattt acgacgacct cctgcggggg ggggacggcg ccattttcct cgaagtgacc
     6721 gcccagggga aggtgctcgg tcaactggcc gatcgccctc cggaaccggc ccggcccggc
     6781 agcgatctct ttttgaccat cgatttagac attcaggccg tagccgaatc ggccctggcc
     6841 ggttcgtgct gcggcgcggc ggtcgccctc gacccccgga cgggtgaggt tctcgccttt
     6901 gtgtccaaac cgggattcga tgccaacgcg tttgtcactg gactttcact ggatcagtgg
     6961 caaaaagtct ccggcgattc cacacacccc aggttgaacc gcaccattca aggactgtac
     7021 ccgccggggt caaccgtcaa acttttgacc gccggggcgg ctctggaatc ccatctcata
     7081 acccccgcca agcgttttaa tgcctgccag ggtgccctgt ggttcggaaa ccggctgttc
     7141 cgctgctgga aaccggaggg gcacggcagt ctggacctgt accaggcgat cgtccaatcc
     7201 tgcgatgtct atttttacca gctgggattg gccttgggcc tggaacggtg gggtgagtac
     7261 gccgccaagt gcggcttcgg ccagaagacc gggatagacc ttactgatga gtgggccggg
     7321 gtcttgccta cagccgccta ttacgctcgc cggtttggcc agaaccagtg gcccaaaggg
     7381 gtgatgctca acctgtccat cggtcagggg gagttcttgg tcacgccctt gcaactggcc
     7441 accttctttg gtgccttggg aaacgatggg gcagtgttcc ggccgcatct tttgaagcgg
     7501 tacgttgacc cggacggact ggaaagagag atcgagccgg tctgcgtgcg tcagttgcct
     7561 ttcagccccg ggactttgcg gttcttaaaa accgccctgg tcggcgtggt aaacgacccg
     7621 cacggtaccg ccacggttgc acaacttccg ggaatcagcg ttgccggaaa aaccggaacg
     7681 gcccagaatc cccacgggtt ggagcacgcc tggtttgcct gttttgctcc ggcagagaaa
     7741 ccggagatcg ctgtcgccgt gttggtggaa aatgccgggc atggcggcac ggtggccgcc
     7801 cccatcgccc gggaaatgct cagagcctat tttaccaaga cacaccccct gctccaacct
     7861 gaagtagcgg ccagtgccgg agtctcagac cgcagccggc gtccggccac ccccggaggt
     7921 gattgaattg gcgggttacg tttagcggct caccgtgcag gtgcagggtg gagcgggcgg
     7981 ccggcggcag agcccgaaat tggtaatcca gaccgtgtcg ccggcgggga ccgaaaagcg
     8041 ccccgtaccg cattgggtgt aatcgggcag tgaatcgaac accgggactg cgttgagctg
     8101 gcggtcggtg cgaatttcct cggcgaaaga gtagcgtccg gaggcatcgg tggtgtcgcg
     8161 cgagttgccg tttaaaagaa tctccacatt gcgcaggaga ttcccggatt ccttggcggt
     8221 taccgtgccc cggaagatga agagactggc cttgatcgtt tttttcgaac actgggtaat
     8281 agaaaatgtt gcgcccagag cgcagagcac cacgatccct gcggcccgga ccgaccgcac
     8341 gaggcttggt tgcgtggtcg tttggttcat agcaggatga agataattcc cgtttctcca
     8401 gggagcaaac agaccggttt actgtaagcg gccatgcgct tgtcacttat ccgcagccct
     8461 gagttcgacc gctggttgac cgtcgacgct ctctttcttt ctctattggg ggtggtgctg
     8521 atttacagcg ccacccatac ggccatcgac cccggagagc accagttgcc gtggaaacaa
     8581 ctcctctggc tgccggtggc gtttttcgga tttttcgctg ccttcttcct gccgctacgc
     8641 ctgcacgagg cgctggctta cgtctattac gcagcggggg ccattttact ggcggcagtg
     8701 ttaattctgg gagaaacgcg catgggcgcc acccgctggt ttgccttcgg gacttttaac
     8761 ctgcagccgt cggagttagc gaagatcacg gcggtgttcg gattggcccg gctgttcgct
     8821 cacgcccgcg aaccggtgac cggcctgcgc cgcttggtgg tggcggtggt catgattctt
     8881 ctgccggtcg ggttgattct gcgccagccc gatttgggca cggctctggt cttctgcgca
     8941 ctggggttgg ccatgctctt ctggtcggga ctgccgctct ggcacttagc gcttttggtt
     9001 tctccgctct tcagtctttt ggccgctttt cactggatca cctgggcttt gtttttcgtc
     9061 ctgctgctct gggtattgta caaaacccgg cccgggtttc tcttcgggac catctgcctg
     9121 gtggctaatc tactcttcgg catggtaacc cctttggtct ggaaccggct ccacgactac
     9181 cagaagatgc gcattctgat tttcttagac ccggggcgcg acccgcaggg agccggttac
     9241 cagttgattc aggccaaggt ggcgatcggt tccggcggca tattcggcaa aggttttctg
     9301 cacggcagcc agaccaagct ggctttcctg ccggcccagc acaccgattt cattttcacg
     9361 gtggtggggg aagaattcgg cctcttgggc gttttgatcg tgttggccct tttgggattc
     9421 ctggtctacc gcggtttgca ggccgccatc cgctgccgca accagtttgc gtcgtacgtg
     9481 gccttggggc tgacgtcgat tctcgccttc cagattctgg tcaacgtcgg tatggccgcg
     9541 ggactaatgc ccgtgacggg cctgccgctc cccttcttga gttacggcgg cacgtcgctt
     9601 ttgaccaact gggtcatcat cgggctttta gccaacatcg ccgtccactg gcaggactac
     9661 tgattaccgt tccggcggcg taaagcgaat ccgcactctg gcgaacgtat tgagcggcca
     9721 ggaggcatcg cgtgccagcg aaaagaggca ccgggggctc agagaggaat ggagccgaaa
     9781 acccaagttc gaaaaagtgc catcaaccca gccttgaaca atttccgtga tgttcactat
     9841 gaccttgttt gattgccgaa aatcgtgcac cactcctacg ccgacggtat caaccaccgc
     9901 gacattgggg tccactacca tctgattgct gggcagccag gtcgtagctg ccgggtggag
     9961 ggcgaaatgg acacgggttc cttcggtagt gtcggcgaag aggcgcattt cgagaagggc
    10021 caggtcaata cgcgctcccc ttagaatagt ggggatttca aatttaacca gagcttgaga
    10081 acggttgctc tgcactacgg cggcgcgttg ggtaacatct agggttatct caaccgcacc
    10141 tgcaggcaag gtcacccaaa aagccagcag tatcgcgaaa ataaccgctg tttggctttt
    10201 gaggatgcga gtttttgtca tttcagcacc accatccttc tggttgcgcg gtgttcgccc
    10261 gcggtgaccg tgtagaagta caccccgctg gccaccggtt tgccctcacg atctttcccg
    10321 ttccatatga cttcgtgact tcccgcatct ctatactcgt tcaccagagt cgccacctgt
    10381 tggccaatga ggttgtagac cttgagggtg acctgcccag gctctggcag tgtgaagcga
    10441 attcttgtta acgggttgaa cgggttcggc tcgttctgct ccaacccaaa ctgaaacccc
    10501 aaagaaactc ctcccgccag tggtttggac atcggctctt ccggcggcag gcaggtcgaa
    10561 tcatcacaga aaggccggaa atccacaggc cccatgaaat tgtgagtgga gtccggggtt
    10621 gtcgtgcccc accagttccc ttcggctttg agcgtagtgg tagacatatt ccaaatgtag
    10681 gtcgaatggc ccggtgggtt attgccgtga ggtgcttcgt aaatagaatt gccaccgggt
    10741 gagttcgcag catcatttcc cagatcaagg ccggacgtgt aactgacaat caccgcagcg
    10801 ccatgacatt ctggaatctg tgggcatgga ttgcgtgtat cgaagtttac cgaactcgaa
    10861 ttgcgcaact tcacgcgtcc cccgccgaag gaatacaagg acgtgttcac accaagcgat
    10921 ggtccgtcga acgtgcaggc accgattgtc gctgccgcac ccgctccgg
//
LOCUS       DBNGZD010000015         1483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1252085, whole genome shotgun sequence.
ACCESSION   DBNGZD010000015 DBNGZD010000000
VERSION     DBNGZD010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1252085"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="AC16GM_00460"
     CDS             complement(<1..389)
                     /locus_tag="AC16GM_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HIR2922181.1"
                     /translation="MGHELPGIGKISPEIFDELIYPHLGKRLKTVLVGPRHGVDIGVV
                     DLSHGQVMATTTDPVFIVPEYGFHRAAWFAIHILCSDAVTSALPLKYLSIDLNLPLKI
                     TKAELTEMWNTIDAECRKMGIAIVSGHT"
     gene            complement(541..1326)
                     /locus_tag="AC16GM_00465"
     CDS             complement(541..1326)
                     /locus_tag="AC16GM_00465"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR2922182.1"
                     /translation="MPPGLGLAASVILGLLVGSFLNVVIYRLPRELSVIKPRSHCPKC
                     CTPIKWYHNVPLFSFLFLGGKCAYCGVKISRRYPLVEAVTAAFFAVACLTIGVRPQLP
                     LVWFLFAAFVAIFFIDLDFQLIPDKITLPGILLGLVFAALTPEIGWLQGLIGLLVGGG
                     AFWFVAVAGEFMFKKESLGGGDIKFAAMVGAVMGWQKLIVIFLGASVLALLWFGISSL
                     LVRGKTQNHQIPFGPYLTVASVVAFFFGDRLIHFYLAYFLSLR"
BASE COUNT          370 a          423 c          438 g          252 t
ORIGIN      
        1 gtgtggccgg aaacgatagc gatgcccatc ttcctgcact cggcgtctat cgtgttccac
       61 atttcggtta actcggcttt agtaattttg agcgggagat tgaggtcaat ggagagatac
      121 ttgagcggca gggcgctcgt gaccgcatcc gagcaaagga tgtggatggc gaaccaggcc
      181 gcccggtgga acccgtactc cggcacgatg aagacggggt cggtggtcgt cgccatcacc
      241 tgcccgtggc tcagatcaac aacgccgatg tcgacgccgt gccgggggcc gactaaaacg
      301 gttttgagcc gtttccccaa atgagggtaa atcagttcgt cgaatatttc cggggaaatc
      361 ttgccgattc ccggtagttc gtgtcccatc ccgcctcctg aacaaaaacc agccctgagc
      421 cgcaaaactt attgtgtgac caccggacag tcaactcgga tgcgcggcgg gggcgctcgg
      481 tacgagtcgt attgaccacg cctacgtgga gaagagtttt ctcaaatggc tttgtcttga
      541 ttaccggagc gaaagaaagt aggcgagata aaagtgaatt agccggtcac cgaagaagaa
      601 tgcgacgacg gaggccactg tcaaatacgg cccgaaggga atctggtggt tctgcgtttt
      661 gccgcgtacc aggagactgg atatcccgaa ccataagagc gccagaaccg aggcacccaa
      721 aaagatgacg atcaacttct gccagcccat aaccgccccc accatcgcgg caaacttgat
      781 gtctcccccg cccaaacttt cctttttgaa catgaactca cccgcaaccg ccacgaacca
      841 gaaggcgccg ccgcctacga gcaggccgat caggccttgc agccagccga tctctggcgt
      901 caaggccgcg aagaccagac cgagcagaat gcccgggagg gtgattttat cggggattaa
      961 ctggaaatcc aagtcgatga aaaaaatcgc cacaaatgcg gcaaagagaa accagaccag
     1021 aggcagctgc ggccgcacac caattgtgag gcaggcgacc gcgaagaagg cggcggtcac
     1081 cgcctccacc aaggggtagc gccgggaaat tttgaccccg cagtaggcgc acttgccgcc
     1141 gaggaaaaga aaactgaaaa gcgggacgtt gtggtaccat ttgatcggcg tgcagcattt
     1201 cgggcaatgt gagcggggtt tgatgaccga cagttcccgc gggagccggt agatcaccac
     1261 gttgaggaaa ctgccgacca gaagacccaa tatgacgctc gccgccagac ccaatcccgg
     1321 aggcatcagc gttcctccag aaggaaaatg ccgtaaagcg caaagagccc cgggaaggtt
     1381 acggccagcc cttgcgccgg cggctgaatg aaatgccagc cgggcggcgc cacggcggtg
     1441 acgcaggact tggtgacggc cagaccggcc gccgcaaaga acc
//
LOCUS       DBNGZD010000016         8859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_835670, whole genome shotgun sequence.
ACCESSION   DBNGZD010000016 DBNGZD010000000
VERSION     DBNGZD010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_835670"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="AC16GM_00470"
     CDS             complement(<1..605)
                     /locus_tag="AC16GM_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2922183.1"
                     /translation="MLFLESVGIALRALVANKLRSILTLVGVVIGVATVIAVTSIIAG
                     MDRYVANQLATLGSNTFMVDKFGIITSDDAWFDAMKRKNLTLEDVAAIRLHCPDCQFV
                     EAQAWAQGRVKYGNQFMSDVDIVGLTHDFNEVTSINVDQHAAGHFLTEPDEERRHQVC
                     ILGYELQDRLFGRINPLGRLVKVRGQEYTVVGLAKKRGSFLG"
     gene            complement(733..1434)
                     /locus_tag="AC16GM_00475"
     CDS             complement(733..1434)
                     /locus_tag="AC16GM_00475"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2922184.1"
                     /translation="MQNGKEALIRTENLWKTYDMGATQVHALQGISFTVQKGEYVAIM
                     GPSGSGKSTLMNLVGCLDTPSQGEYELNGQLVSKLTDDELARIRNKEIGFVFQTFNLL
                     ARATALHNVELPLIYNGTPSAERLKRAKAALNLVDLADRMQHKPNELSGGQRQRVAVA
                     RALVNNPSIILADEPTGNLDTKTGVEIMQLFDRLHKQGNTIIIVTHEHDVAQYADRVV
                     HIRDGKVEKDERVKR"
     gene            complement(1434..2756)
                     /locus_tag="AC16GM_00480"
     CDS             complement(1434..2756)
                     /locus_tag="AC16GM_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2922185.1"
                     /translation="MKKRSKILLIAGGALVVIIVVVLNLVKSGSAVVKVQTEKVEKRD
                     ITSIVTATGKVEPKTTVKISANISAKITRLPVKEGDRVKKGQVLVQLEPTRYQAAVNQ
                     YAASLAAARAQLRLAESKLAESRQLRDRTQSLFEKNLASKEQHDAVVTAYAVAEATVD
                     NYQHQVEQTEAALAQAQDDREKTTITAPMNGVVTELKAEAGEVVIPGTMNNPGTVILV
                     VSDLSEIEVKVDVDETDIALVKLGQPAKIEIDAFPDTAYHGRVTEIGNSAQVKGLGSQ
                     DQVTNFQVKVLLVDTVPNIKPGMTASVDVTTDSRSGVVAVPIQAVVMREEKDLKKKST
                     AEDRKKAKQGETVAEAAPVDTAAGKNPKGKKAKPLEGVFVVREGTAHFVSVKTGISDQ
                     ENIEIASGLEPGDEVVVGSYKILRTLEDEAKVKKEVMTASAAAGEEKK"
     gene            complement(2842..4167)
                     /locus_tag="AC16GM_00485"
     CDS             complement(2842..4167)
                     /locus_tag="AC16GM_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2922186.1"
                     /translation="MRKPLSGIFVLTLIVSGTAAAQEAPASGGLTLDECIKIALKENP
                     TVIAAQHSYTAAKGEVYRAWGGLLPSVNLGLDMSFFKSGFPRINQATGQFQQARWSLT
                     YRPSVSLNQTLFNGGANYYNVAWRADEKEAAYQALQSTRQQMVLSVKENYFALLKAKM
                     LLDIQKDAVKRGEEQLKVAQTRYDLGSASLSDVLKAKVLYGNDQLALVSAENSYELAK
                     ANLNFILNRDVNTPVEIEEKLPDVQNPYTLEQALAQAKISHPGLRQARADYSASRAQV
                     GIARAAYVPEVSWGLTRSWSVPDTRVERLLKFDQVYGTWSVFISANFPLFNGFNREAT
                     FLASRVNSDTKKENLARTENQVALEVKQAFLGIAESQKKLQVSEENEKSSQEDLNLAK
                     EKYNLGAATILEVLDAEVNFKTVQSNRVQALFDYHLAIANLEKAMGRKY"
     gene            complement(4288..5025)
                     /locus_tag="AC16GM_00490"
     CDS             complement(4288..5025)
                     /locus_tag="AC16GM_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR2922187.1"
                     /translation="MEGQATVMPATQEPAMNLLARIWNVWVAPKKAFANVKLYPRWVV
                     PFFLFLAIHFAVWPVVAPQVAKDAEQIVNTSPRFENVPAEQKEKMIAGFHQGLTVKNV
                     IQGVLISTIITVLMGALFLFFTNVLLGGQVKYWQTTAVFIYSGFILIPEYVLKLPLIF
                     AKKSALVATGPGLFVSAGEGFGAHFLSAIDFFGLWQAGVVAVGLGVLAGSTTKKAGIW
                     VFSAWGLWVIIKAALSYFSGGLSRLSF"
     gene            complement(5283..7619)
                     /locus_tag="AC16GM_00495"
     CDS             complement(5283..7619)
                     /locus_tag="AC16GM_00495"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR2922188.1"
                     /translation="MKTFTIFLTSLIFLTLATSVWAGEGDVKLPPFDKTINTSLYFVL
                     LSAVIALLYGLYLVRKTLAREQGTAKMIEVGRAIQEGAKAYLTRQMKVLSLFIILLTV
                     VIFFVYKGLYLLEDGTTNWKLVWGIALAFLAGSSLSAMTGLIGMSVAVRSNTRVAHAA
                     RTSFKQALESAFEGGTVTGMFTVGMGLVGATIIFMLFKEQAMQVLIGFGFGGSLVALF
                     MRVGGGIYTKAADVGADLVGKVEAGIPEDDPRNAATIADNVGDNVGDCAGMAADLFES
                     YEVTLVAAMILGAAYSALHPEQSGLALKMIVFPLLVRAVGVFASIFGTWSVRGKDRDD
                     FNPMRPIQNGFVVSAVTATLGFGLINYFFIKTPDGSADFRFFWATFSGIILAVVISYL
                     TEYYTALEYSPVKETARATKTGPATTILAGFSEGLESTVVAIMAIAGAIVASALIFKG
                     DLALAAYGIALCGMGMLTNTGVIVSMDSYGPISDNANGIFEMSKVLEEDKNPKSHMIV
                     AKLDAVGNTTKAVTKGFAIASAVVAAVALFRSFSSDVNILDPALNFISKGIQVNLPYV
                     FVGLLIGGAMPFLFCSVAIRAVGRAAFQVVEEVRRQFREHPGIMKGTEKPEYYRCVDI
                     ATSAAQKELITPGILAVSAPVAVGFLLGVEALGGFLAGIILTGQLLAVLLSNAGGTWD
                     NAKKSVEAGLYGGKGSEAHKAGIIGDTVGDPFKDTAGPSLNPLIKVMNLVSLLILPLI
                     VGRGKLGGTVATVTAVGCLVLLVISLLISKRSTQAKTA"
     gene            complement(7792..>8859)
                     /locus_tag="AC16GM_00500"
     CDS             complement(7792..>8859)
                     /locus_tag="AC16GM_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2922189.1"
                     /translation="EASKLGYLINLNTDLHLNKPELDVDIDRERASSLGVSVMDIGST
                     LETFLGGRVVTNFKRAAKQYDVITQLRASERATPGTIEDLYIRSNGGLVQLANLVQIR
                     ETVAPKELNHYSRVRSATVSAGLMPGVSLGRALNDLDKIVENDLPPDIKRVLAGQSRE
                     FRESSTAIYFLFLLAVAFIYLVLAAQFESFIHPFTILLSVPLALVGALVSLFVFGQSL
                     NIYSQIGLVMLIGLVTKNSILIVEFSNQLRLRGEELFEAVVKASRIRLRPILMTSFAI
                     IFGVLPIAIGLGAGGEARRPLGIAVVGGILFSTFLTLVLVPVVYTLLARFTRREAAKG
                     ETPEETPATSEPHLSPAAVTG"
BASE COUNT         1825 a         2662 c         2495 g         1877 t
ORIGIN      
        1 cccagaaacg agccgcgctt cttggccagc cccaccaccg tgtactcctg gccgcggact
       61 ttcaccagcc ggcccagagg attgatcctg ccgaagagcc gatcctgcag ctcgtaaccc
      121 agaatgcaga cctggtggcg ccgttcctcg tccggttcgg tgaggaagtg cccggccgcg
      181 tgctggtcca cgttgatcga ggtcacctcg ttgaagtcgt gcgtcaggcc gacgatgtcc
      241 acatccgaca taaattgatt gccgtacttc acccggccct gcgcccatgc ctgcgcctcc
      301 acgaactggc agtccggaca gtggaggcga atggctgcca catcctctaa ggtgaggttc
      361 ttgcgcttca tcgcgtcgaa ccaggcgtcg tcgctcgtga taatgccgaa cttatcgacc
      421 atgaaggtgt tggaaccgag agtggccagc tggttggcca cgtagcggtc catcccggcg
      481 atgatcgagg tgaccgcaat caccgtcgcc acgccgatca cgacgccgac caaagtcaga
      541 atcgagcgga gcttattggc gaccagcgcc cggagcgcga tgccgacgga ttcaaggaag
      601 agcatggaaa attaactgcc ctatggcaca ggttggtttt cgcccgccgt gtgtctgtcg
      661 aaggcggatc gaaccgtaac ctgcttttgc gcgggataca gagaccatcc cgtgcgacga
      721 agttttatcc aatcagcgtt tcacccgctc gtctttttcc accttcccgt cgcggatgtg
      781 gacgacgcgg tcggcgtact gggccacgtc gtgctcgtgg gtgacgataa tgatcgtgtt
      841 gccctgcttg tggagccggt cgaagagctg catgatttcg accccggttt tggtgtccag
      901 gttgccggtc ggctcgtcgg ctaagatgat ggagggatta tttaccagcg cccgggcaac
      961 ggccacccgt tgcctctggc cgcccgaaag ttcgttcggc ttgtgctgca tccggtcggc
     1021 cagatcgact aagttcagcg cggccttcgc ccgtttcagc cgttcggctg acggtgtacc
     1081 gttgtaaatc aagggcaatt cgacattgtg taaggccgtg gcccgggcga ggagattaaa
     1141 cgtctgaaag acaaagccga tttctttgtt ccggatgcgg gccagctcgt cgtcggtcaa
     1201 tttgctcacc agctggccgt tcaattcgta ctcgccctgc gacggcgtgt ccaggcagcc
     1261 gaccaagttc atcaaggtcg atttgccgga accggagggt cccatgatgg ccacgtactc
     1321 gcccttctgg acggtgaagg aaattccctg caatgcgtgt acctgcgtgg cgcccatatc
     1381 gtaggttttc cagagatttt ccgtgcggat gagggcttct ttaccgttct gcattacttc
     1441 ttctcctcgc cggccgcagc cgacgcagtc atcacctctt tttttacctt cgcttcgtct
     1501 tcgagggtgc gcaggatttt gtaactaccg accaccactt cgtcacccgg ctcgaggccg
     1561 gaagcgattt cgatattttc ctgatcggag atgccggttt tcaccgacac gaaatgggcc
     1621 gtcccctccc gcaccacgaa cacgccctcc aacggtttgg cctttttgcc cttcgggttt
     1681 ttgccagccg cggtgtccac cggtgccgcc tcggccacgg tctcgccttg cttggccttc
     1741 ttgcggtcct ccgccgtcga ttttttcttt aaatcttttt cttcccgcat taccacggcc
     1801 tggatcggga ccgccaccac cccggagcgg gagtcggtgg tgacgtccac cgaggcggtc
     1861 atgccgggct tgatattggg caccgtgtct acgagcagaa ccttcacctg gaagttggtc
     1921 acctggtcct gcgagcccaa ccctttcacc tgggccgagt tgccgatttc ggtcaccctg
     1981 ccgtgatacg cggtgtccgg gaaagcgtct atttcgatct tcgccggctg gcccagtttc
     2041 accagcgcga tgtcggtttc gtctacatcc actttgactt cgatttcaga gagatcggag
     2101 accaccagga tgaccgtgcc cgggttattc atcgtgccgg ggatcaccac ttcgcccgcc
     2161 tcggccttga gctcggtgac caccccgttc atcggcgcgg tgatcgtggt tttctcccga
     2221 tcgtcctggg cctgtgctaa agccgcctcg gtctgctcca cctgatgctg gtagttgtcg
     2281 acggtggctt cggccaccgc gtaggcggtc acgaccgcat cgtgttgctc tttcgatgcc
     2341 aggttttttt cgaaaagcga ctgggtccgg tcgcgcaatt gccgggattc ggccagcttc
     2401 gattcggcca gccggagctg cgcccgcgcc gccgccagag acgcggcgta ctggttcacc
     2461 gccgcctggt agcgtgtcgg ctccagctgc accagcacct ggcctttctt cacccggtcc
     2521 ccttctttga ccggcaggcg ggtgattttg gccgagatgt tggccgaaat tttcaccgtg
     2581 gttttcggct ccaccttgcc ggtggcggtg acgatggagg tgatgtcccg cttctccact
     2641 ttttcggtct gcacctttac cacggcactc cccgatttga ccaggttcag caccaccacg
     2701 ataatgacga ccaaagcccc gccggcaatc aagagtatct tacttcgttt cttcatctct
     2761 ctctccttaa gaatacggca aaaccgtcat tgcgtgctct gcctgcagaa actgcgccgg
     2821 gcaatttccg caagcaaaaa atcagtattt cctccccatc gctttttcta aattggcaat
     2881 ggccagatgg tagtcgaaca aggcctgcac ccggttcgac tgcacggttt taaagttcac
     2941 ttccgcatcc agaacttcca ggatggtcgc cgcgcccagg ttgtacttct ccttggccag
     3001 gttcaagtct tcctgcgacg acttttcgtt ctcctcgctc acctggagtt tcttctgcga
     3061 ctcggcgatg cccagaaacg cctgcttgac ttccagcgcc acttgatttt ctgtccgggc
     3121 taaattttcc ttttttgtgt cgctattgac ccggctggcc aaaaaggtcg cctcccggtt
     3181 gaacccgtta aagagtggga agttggccga gatgaagaca ctccaggttc cgtacacctg
     3241 gtcgaatttc aaaagccgtt cgacgcgggt atccggcacc gaccaggagc gggtgagccc
     3301 ccaggagacc tcgggaacgt aggcggcgcg ggcgatgcct acctgtgccc ggctggcgct
     3361 gtagtcggcc cgcgcctggc gcaatccggg gtgggaaatc ttcgcttgcg ctaaggcctg
     3421 ctccagggtg tatggattct ggacatcggg gagtttttct tcgatttcca ccggagtgtt
     3481 cacgtcgcgg ttcaggatga aattgagatt cgccttggcc agctcgtagc tattttcggc
     3541 ggacactaag gccagttggt cattgccgta gagcaccttc gccttcaaaa cgtccgaaag
     3601 cgacgccgag ccaagatcgt agcgcgtctg ggccactttc aactgttcct cgccgcgctt
     3661 gaccgcatcc ttctgaatgt ccaaaagcat cttggctttg aggagcgcga agtagttctc
     3721 tttgacgctc aaaaccattt gctggcgggt actctgcaag gcctggtagg cggcttcttt
     3781 ttcatccgcg cgccaggcta cgttgtagta gttagcgccg ccgttgaaaa gcgtctggtt
     3841 gagcgagacg ctgggccggt aggtcagact ccagcgcgct tgttgaaact gcccggtcgc
     3901 ctggttgatc cgggggaacc cggacttgaa gaagctcatg tccaacccca ggtttaccga
     3961 ggggagcagc ccgccccaag cccggtagac ttcccctttg gcggcggtgt agctgtgttg
     4021 ggctgcaatg acggtcgggt tttccttgag cgcaattttg atgcactcat ccagggtcaa
     4081 cccgcctgag gcgggcgctt cctgggcggc tgccgtcccg gacacgatca aagtgagaac
     4141 aaaaatgcca ctcaatggtt ttcgcattca gactcctctt ggttctttct tttatccttt
     4201 acggatgacc gaccgcgtaa gttccaatca agggtcacga ccaacgaaaa atcgtcttcc
     4261 tacgcattct cctcctcgtt tatgcaacta aaaagatagc cgggacagtc ctccggagaa
     4321 ataactcaag gcggccttaa taatcaccca gagcccccac gcgctgaaga cccagatgcc
     4381 cgctttcttg gtcgtactcc cggccagcac ccccaagccg acggcaacga ccccggcctg
     4441 ccagaggccg aagaaatcaa tggctgaaag gaagtgcgcg ccgaacccct cccccgccga
     4501 cacaaagagc cccggcccgg ttgccaccag ggcactcttt ttggcgaata tcaacggcaa
     4561 cttcagcaca tactcgggaa tgagaatgaa tccagagtag atgaagaccg ccgtcgtctg
     4621 ccagtacttc acctggccgc ctaaaaggac attggtgaag aagaggaaca aagcacccat
     4681 cagcacggtg atgatggtcg atatcagcac accctggatt acgttcttca ctgtcaaacc
     4741 ctggtggaag ccggcgatca ttttctcttt ctgctctgcc ggcacatttt caaagcgcgg
     4801 cgaggtattc acaatctgct ccgcgtcttt ggccacctgg ggagccacca ccggccagac
     4861 ggcaaagtgg atcgccagaa aaagaaaaaa cggcaccacc cagcgcgggt agagcttgac
     4921 gtttgcgaat gcttttttcg gcgccaccca gacgttccag atgcgcgcca aaaggttcat
     4981 cgccggttcc tgggtcgccg gcatcacggt cgcttgtcct tccattcgct ctcctttgcc
     5041 tccgtgttag gttgcagaac gtaagaaacg aaaagaagtt tcaaaaatca aaccgggcgt
     5101 tctctctcta aaggtgggga tcagaatagg gatttcgttc tctcccattg aataggacga
     5161 ggtggcagcg caagcggcga ccgagggcgg tcgccctacg cgaggggatg gctgatcacc
     5221 cgcacggtga tataacatcc gaccgcgtgg gcgcggcgcc ccccgccgcg ctgaaaagtt
     5281 tgctatgcgg ttttcgcctg cgtactgcgc ttactgatca gaagcgagat gaccaggaga
     5341 accaagcagc cgacggcggt cacagtcgca accgtcccgc ccagtttccc ccggccgacg
     5401 atcagaggga gaatcaaaag cgagactaag ttcattacct tgatcaaggg gttgagcgac
     5461 ggcccggcgg tgtctttgaa cgggtcgccc accgtgtcgc cgatgattcc ggctttgtgc
     5521 gcttcggagc ccttgccgcc gtagagcccc gcctccaccg attttttggc attgtcccag
     5581 gtcccgcccg cgttggagag gagcaccgcc aacaactgtc cggtgagaat gatcccggcc
     5641 agaaatccgc ccagcgcttc cacacccaga aggaagccga ccgccacggg cgcggaaacc
     5701 gccaggattc cgggagtaat caactccttc tgcgctgcgg aagtcgcgat gtcaacgcag
     5761 cggtagtatt ccggtttttc cgttcccttc ataattcccg gatgctcccg gaactgccgc
     5821 cgcacttctt ccacgacctg aaaggcggcg cggcccacgg cgcggatcgc caccgaacag
     5881 aagaggaacg gcatcgctcc gccgatcaga agccccacaa agacgtaggg caggttcacc
     5941 tggatgccct tgctgatgaa attcaacgcc gggtcgagga tgttcacgtc cgaagagaag
     6001 gagcggaaga gcgccaccgc cgccaccacc gccgaggcga tggcgaaccc tttggtcacg
     6061 gccttggtcg tgttgcccac cgcgtccagc ttggccacga tcatatgcga cttgggattt
     6121 ttgtcctctt caaggacttt agacatttcg aagatgccgt tggcattgtc tgaaatcggc
     6181 ccgtaggaat ccatcgagac gatgacgccg gtattggtga gcatccccat cccgcagagc
     6241 gcaatcccgt aggccgccag agccaggtcg cctttgaaga tgagggccga ggccacaatc
     6301 gcccccgcaa tggccatgat cgccactacc gtcgattcca gcccttcgga aaaaccggcc
     6361 agaatggtgg tggccggtcc ggtcttggtc gcccgcgccg tctctttcac cggactgtac
     6421 tcaagggcgg tgtagtattc ggtcaggtag gaaataacga cggccaggat gatgccggag
     6481 aaggtggccc agaagaagcg gaagtcggcc gacccgtccg gggttttgat gaagaagtag
     6541 ttgatgagtc cgaagccgag ggtggcggtc accgccgaga ccacaaaacc gttttgaatc
     6601 ggccgcatcg gattgaaatc gtcccggtct ttgcctctga cgctccaggt gccgaaaatg
     6661 gaagcgaaaa cacccaccgc ccgcactaac aggggaaaga cgatcatttt caaagccagg
     6721 cccgactgct ccggatgcag ggcggagtag gcggcgccca agatcatcgc ggcaacgagc
     6781 gtgacctcgt acgattcgaa caggtcggcg gccatcccgg cgcagtcgcc cacgttgtcg
     6841 ccaacgttgt cggcaatggt ggccgcgttg cgcggatcgt cttcgggaat gccggcttcg
     6901 actttgccga ccagatcggc ccccacgtca gctgctttgg tgtaaatccc cccgcccacg
     6961 cgcatgaaga gcgccacgag agacccgccg aaaccgaagc cgatcaaaac ctgcatcgcc
     7021 tgctctttaa agagcatgaa gatgatggtg gcgccgacca accccatccc aacggtgaac
     7081 ataccggtaa ccgtgccgcc ttcgaaagcc gattccagcg cctgcttgaa tgatgtccgg
     7141 gcggcatgcg ccacgcgggt attggagcgc accgccaccg acatcccaat taagccggtc
     7201 atggccgaaa gagatgaacc ggccaaaaag gccagcgcaa tgccccagac caatttccag
     7261 ttcgtcgtgc cgtcttccaa gaggtaaagc cccttgtaga cgaagaaaat caccaccgtc
     7321 aaaagaatga tgaaaagcga caacactttc atttgccggg tgaggtaggc cttggcgcct
     7381 tcctggatgg cgcgccccac ttcgatcatt ttggccgtcc cctgttcgcg ggccagcgtt
     7441 ttgcgcacca ggtacaggcc gtacaggagg gcgataactg ccgacaggag aacgaaatag
     7501 agcgaggtgt taatggtttt gtcgaaaggc ggaagtttga cgtccccttc ccctgcccaa
     7561 accgaagtgg caagagtcaa aaagataaga gaagtcaaaa agattgtgaa agttttcatt
     7621 tggaaaaagc ggcctttcct ccactgaagc tgcatgcacc tctccttctt tggttacccg
     7681 ctctgcccca agtttcgtaa tctctacagc gagcagaata taggaacggt tttcttatat
     7741 gcaagtcaaa atttgtcttg ctcgaaaagt aaagaaaccg cccgacgggg ctcatccggt
     7801 tacggcagcg ggactgagat gcggttctga ggtagcggga gtttcttcgg gagtttcccc
     7861 cttcgccgct tcccgccggg tgaagcgcgc cagaagcgta taaaccacgg gaaccaagac
     7921 taaagtcaag aacgtggaaa acaggatgcc gcccaccacg gcaatgccca gcgggcggcg
     7981 ggcttcaccg cccgccccca gtccaatggc aatcggcaga acgccgaaga tgatggcaaa
     8041 ggaggtcatc aaaatcggac gcaagcggat gcgcgaagcc ttgaccaccg cttcaaaaag
     8101 ttcttccccc cggaggcgca gctggttgga aaactccaca atcaaaatgg aatttttggt
     8161 caccagcccg atcaacatca ccagtccaat ctgggaatag atgttcagac tctgcccgaa
     8221 aacgaaaagc gagaccaagg ctcccaccaa agcgagcgga acggagagca gaatggtgaa
     8281 aggatgaatg aagctttcga actgggcggc caaaacgaga taaatgaaag cgacggccag
     8341 aaggaaaaga aaatagatgg ccgtgctgga ttcgcgaaac tcccgcgact gccccgccaa
     8401 cacccgtttg atgtcgggcg ggaggtcatt ttcgacaatt ttatccaaat cgttcagggc
     8461 ccttcccaaa ctaactccgg gcatcagacc ggcggagacg gtggcggagc ggacgcggct
     8521 gtaatggttc agctccttgg gagccacggt ttcacgtatc tgcaccaggt tagccagttg
     8581 gaccaggccg ccgttgctgc ggatgtagag gtcttcgatg gtgccggggg tggcgcgttc
     8641 ggaagctcgg agctgggtaa tgacgtcgta ctgcttggcg gctcgcttga aattggtcac
     8701 cacccggccg cccagaaagg tttccagcgt cgaaccgata tccatcaccg atacgccgag
     8761 gctcgaagcg cgctcgcggt cgatgtcgac atccagctcc ggcttgttca ggtgcaggtc
     8821 ggtgttcaga ttgatgagat agccgagctt cgacgcctc
//
LOCUS       DBNGZD010000017         3927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_905316, whole genome shotgun sequence.
ACCESSION   DBNGZD010000017 DBNGZD010000000
VERSION     DBNGZD010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_905316"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1335
                     /gene="serS"
                     /locus_tag="AC16GM_00505"
     CDS             64..1335
                     /gene="serS"
                     /locus_tag="AC16GM_00505"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR2922190.1"
                     /translation="MLDLKFIRERLEQVKDGVRHKNHQVDIDQLLSLDEERRRLIARS
                     EELKRKRNETTEEIALLKKEKKDAEKLITEMRQVGQEIAILDEQLKQLEQEVETLLWR
                     VPNLPHASVPVGKDETANVEVRRWGKLPEFPFTPRPHWELGQALGILDLPRAAKISGS
                     GFITFTGIGARLERALISFMLDLHTQKHGYTEVLPPFLVTTAAMYGTGQLPLLAQDMY
                     RLEQDDLWLVPTAEVPVTNLFRDEILEALDLPIYRVAYTPCFRREAGSYGKETRGIIR
                     VHQFDKVELVKIVRPEHSYAELEKLVTDAEEVLQLLGLPYRVRLLSTGDLSFAAAKCY
                     DLEVWAPGVESFLEVSSCSNYEAFQARRMNVRFRPAPNAKPDYPHTLNGSGLALPRTL
                     AALLENYQTEKGTVRIPDVLQPYLGGLAEIT"
     gene            1336..2577
                     /locus_tag="AC16GM_00510"
     CDS             1336..2577
                     /locus_tag="AC16GM_00510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2922191.1"
                     /translation="MTNSEINRPQATGVPMVFRLFLLFGILVIALFFIWYTQNVIAKL
                     KEDQAKVVDMYARLWQLVTQEPTTGPAVSFIFEEIIQKSNIPIIITDSGGSIQFWRGL
                     EQVPEKDRSPANLARVHQMLEKFDHQRGPLPIRYGDRIIYYFHYGDPKLVRQLQQMPW
                     ILLSLFGLFLVVAFIGYRNIKRSEQRFIWVGMAKETAHQLGTPLTSLLGWLEILKSHT
                     AETGQAENPSLAEIAERMEGDIVRLQRVAQRFGQIGSLPELKPVDLNALVHEAASYFR
                     TRLPGSGQGVVIKENLKELPPVEANPELLSWVIENLLKNALEAVDASTGHIEVTSRMS
                     KDEKTVCITVTDNGKGIPSREQKKVFHAGYTTKKRGWGLGLTLAKRIVDEYHKGKLAL
                     VESIPNQRTEFQISLPLRNPS"
     gene            complement(2705..3472)
                     /locus_tag="AC16GM_00515"
     CDS             complement(2705..3472)
                     /locus_tag="AC16GM_00515"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HIR2922192.1"
                     /translation="MLLKDKVALVTGASRGLGAAIATALGQAGAKIVVNYFKNKDKAE
                     QVAQKIKSSGSDAIAIYADVRNRKAVEALVAEATKKFGRIDILVNSALYNYSFDPVRR
                     KTFLQINWQDYQEQINGTLKGAFNCCQAVLPQMIQRKSGKIIQILTNLVNNPVVQYHD
                     YTTAKAALVGFSRNLAAELGPYGITVNMIAPGLTVPTDAAAATTEEVKQLVASITPLR
                     RVGSPEEVAQVVVAWASDQMNWATGQYITADGGLVMA"
     gene            complement(3553..>3927)
                     /locus_tag="AC16GM_00520"
     CDS             complement(3553..>3927)
                     /locus_tag="AC16GM_00520"
                     /inference="COORDINATES: protein motif:HMM:NF017038.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2922193.1"
                     /translation="GWRFKTHLDSEHPVSFGESRDGHTYPKPDNVLTFDKLSSVYASG
                     NRSRDSQPNHLRVTIDVPEAIGETWIHMCPAHVYEWENDGSGQKKIRINPSNCIQCGA
                     ISAKGGRLTPPEGGSGPEYTLT"
BASE COUNT          835 a         1113 c         1154 g          825 t
ORIGIN      
        1 cggcgaggca ctggcggccg acgcagtcca gggttctcgg taaacaaatt ttctcatccc
       61 gtcgtgctgg atctcaaatt catccgcgag cgcctcgaac aggtcaaaga cggggtgcgc
      121 cacaaaaatc atcaggtcga tatcgatcag ctgctttctt tagacgaaga acggcggcgg
      181 ctgatcgccc gctccgagga attgaagcgc aagcgcaatg agacgaccga agagattgca
      241 cttctgaaaa aagagaaaaa agatgccgaa aaattgatta ccgaaatgcg ccaggtggga
      301 caggagatag ccatcctgga tgaacagttg aagcaattgg aacaggaagt cgagacgctc
      361 ctctggcggg tgccgaatct cccccatgcc tcggtgccgg tggggaaaga cgagacggcc
      421 aacgtggagg tgcggcggtg gggaaagctc ccggaattcc cctttacccc gcggccccac
      481 tgggagctgg gtcaggcatt gggcattctc gatctgcccc gggcggcgaa aatctccggt
      541 tcgggattca tcaccttcac cggtatcggc gcaaggctgg aacgggcact gatttcgttc
      601 atgctcgatc tccacacgca gaagcacggc tacacggaag tgctgccgcc cttcctagtc
      661 accaccgccg caatgtacgg cacgggtcag ttgccgcttc tggcgcagga tatgtaccgc
      721 ctggagcagg acgacctctg gctggtaccc acggcggagg tgccggtgac gaatctgttc
      781 cgcgatgaaa tcctggaagc ccttgacctc cccatctacc gggtagccta caccccctgc
      841 ttccggcggg aggctggctc ctacggcaag gaaacgcgcg gcattatccg ggtacatcag
      901 ttcgacaaag tggaattggt caaaattgtc cgtccggagc attcgtatgc tgaactggaa
      961 aaattagtga ccgacgctga ggaagttctc cagttgctcg gtctgccgta ccgcgtccgt
     1021 ctcctttcca ccggcgacct ctcttttgca gcggccaagt gctacgacct cgaagtctgg
     1081 gcgccgggcg tcgagagttt tctggaagtt tcctcctgtt cgaattacga agcgttccag
     1141 gcccggcgga tgaatgtgcg cttccggccg gcgccgaacg ccaagcccga ctatccccac
     1201 acgctcaacg gctccggact ggccctgccg cggacgctcg cggccctttt ggaaaattac
     1261 cagaccgaaa aagggacggt gcggatcccc gacgtgctcc agccgtacct cggcggttta
     1321 gcggagatta cgtaggtgac caactccgag atcaaccggc cacaggccac cggggtgccg
     1381 atggtcttcc gcctcttttt gctcttcggc attctggtga tcgctctgtt tttcatctgg
     1441 tacacccaga acgtcatcgc gaaactgaag gaagaccagg ccaaagtagt ggacatgtac
     1501 gcgcggctgt ggcagctggt gacccaggag ccgaccaccg gaccggcggt ttcgttcatt
     1561 tttgaagaga tcatccaaaa gtcaaacatt ccgattatca tcactgactc aggggggagc
     1621 atccagttct ggcggggcct ggagcaagtg ccggagaaag accgctcgcc ggccaacctg
     1681 gcccgggtcc accagatgct ggagaagttt gaccaccagc gcgggccgtt accgatccgc
     1741 tacggcgacc ggatcatcta ctactttcac tacggcgatc cgaaactggt gcggcagcta
     1801 cagcagatgc cctggattct gctctcgctc tttggacttt tcttggtcgt cgccttcatc
     1861 ggttaccgga acatcaagcg gtccgagcag cgtttcatct gggtggggat ggccaaggag
     1921 acggcccacc agttggggac gcccttgacc tcgcttttgg ggtggctcga gattctcaaa
     1981 tcccatacgg cagaaacggg tcaagccgaa aatcccagtc tggcggagat tgcggagcgg
     2041 atggaaggcg acattgtccg gctccagaga gtcgcccagc ggttcgggca gatcggttcg
     2101 ctccccgaac tcaagccggt tgatctgaat gcgctcgttc acgaggctgc cagctacttc
     2161 cgcacccgct tgccgggcag cggccagggg gtcgttatca aggaaaacct gaaggaattg
     2221 ccccccgtcg aagccaatcc ggaactcctg tcatgggtga ttgaaaacct tctcaaaaat
     2281 gctctggagg cggtggacgc tagcacgggg cacattgagg tgaccagccg catgtcaaaa
     2341 gacgaaaaaa cagtctgcat tacggtcacg gacaacggga aggggattcc ctcccgggag
     2401 cagaaaaaag tttttcacgc cggctacacg acgaaaaagc gcggatgggg gttggggttg
     2461 actttggcca agcggattgt tgacgagtac cataagggaa aactcgcgtt ggtggagagc
     2521 atcccgaacc agaggaccga gtttcagatt tccctgccgc tgcggaatcc gtcatagatt
     2581 ccagccacga cgtatccgcg acgccgctcg ggagagcggc gctacagaaa ttcagagaaa
     2641 ttattttaag aactttagat tctgcagcgg ttaggccggc aggcctgacc cgatcccgac
     2701 tttgtcatgc catgaccagt ccgccgtccg cagtgatata ctggccggtg gcccagttca
     2761 tttgatccga ggcccaagcg acgacgacct gtgctacctc ctccggcgag ccgacccggc
     2821 gcaacggcgt gattgaagca accagctgct tgacctcttc ggtagtggct gcggcggcgt
     2881 cggtgggcac ggtcaagccc ggggcgatca tattcaccgt aatgccgtag gggccgagtt
     2941 ccgccgccaa gttgcgcgaa aagccgacta aggcagcttt ggccgtcgtg taatcgtggt
     3001 actgcaccac cgggttattg accaggttgg tgagaatctg gatgattttt cccgacttgc
     3061 gctggatcat ctggggcagg accgcctggc agcagttgaa ggcgcccttg agcgtgccgt
     3121 tgatttgctc ctggtaatcc tgccagttga tctgcaggaa ggtcttgcgc ctgaccgggt
     3181 cgaaggagta gttgtagagg gcgctattca ccaagatatc aattctgccg aatttcttcg
     3241 ttgcttcggc gaccaaggct tcgactgctt tccggttacg gacgtcggcg taaatagcaa
     3301 tggcatcact accagaactt ttgattttct gcgccacctg ttcggctttg tccttgttct
     3361 tgaaatagtt gacgacaatt ttcgcgccgg cttgccccag agcagtggcg atagcggcgc
     3421 ccagtccgcg ggatgcgccg gtgactaagg cgactttatc cttcaaaagc atcattcctc
     3481 cagttggttc tcagggtcat taaaatatac ctatgggtgc tggcgcgcaa agcgacctat
     3541 catcaggctc aattacgtca gcgtatactc aggcccggag ccaccttcag gaggggttaa
     3601 acgaccgcct ttcgcggaaa tggcgccgca ctgaatgcag ttggaggggt ttatccgaat
     3661 cttcttttgg cccgaaccgt cgttttccca ttcgtaaacg tgggccggac acatatgaat
     3721 ccaggtttca ccgatggctt cgggaacgtc gattgtcacc cgcaggtggt tcggctggct
     3781 atcccgactg cggttgccgc tggcgtagac gctggaaagt ttgtcgaagg tcaagacatt
     3841 atctggtttc gggtaggtgt gcccgtcccg cgactcccca aaagagacgg gatgctcgct
     3901 atcgagatga gtcttgaacc gccaacc
//
LOCUS       DBNGZD010000018         3784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_2841, whole genome shotgun sequence.
ACCESSION   DBNGZD010000018 DBNGZD010000000
VERSION     DBNGZD010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_2841"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="AC16GM_00525"
     CDS             <1..238
                     /locus_tag="AC16GM_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922194.1"
                     /translation="LFANPQHLESSGDDPNHLQQNYIMGFVFTDERGNSNLSITEEWY
                     KDFNEVYETFNPEDCVREEDMQVDNRYVVGSETD"
     gene            250..2325
                     /locus_tag="AC16GM_00530"
     CDS             250..2325
                     /locus_tag="AC16GM_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002752900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HIR2922195.1"
                     /translation="METSAKSRSWKVEEQQLLFSEDSLLPDTLPHLRELQPSRNLAAI
                     FDECHNYIYANEGLLKDKIFHEIVKLLVMKLYDEQNVAQGILQFGVTLSEYRSILANT
                     SNTFEERLNTLFAIVRSKYKVFFADEMLKLRPLTLAYVVGRLQNINLTKTPGDIKGEA
                     FQSFVYRHQRGDRGEFFTPHPIVRLAVEMIAPKPDEKVIDPACGSGGFLVQTIAYVHR
                     NYPNIEKAAYIQEHISGIEFNPDVALSAMIRLAFEGGTGTEITCTNSLTENERLNKAF
                     DVVLTNPPFGSKGKVEDQKILKSYVLARRWNKLSNNDWEPTKTVLPGQSPEILFIEKC
                     LNLLRPGGRMAIVLPDGLLQNISNSHIRFWIRSQAKVLGVVSIPQEAFVPYGTGIKTS
                     LLLVQKLPASSRGDCFMARIQKIGYDVKGQLLYRRDESGRLIRSSSGLPVVDDDVEEI
                     ATTFNAFKKDGALCQNRENIYTASEEFLNSRMDAEHYQPDDKKLIEHIRSLGAKPLGE
                     IADILTETDDFRLESDSEIRYIAISDVDARTMQVVSQQIIKAHEAPSRATYRLRKGDI
                     ITASSGASTGTQRQATALITEDEDGAICSNGFAVIRNIQGVQPLFLLAYMRTEYYLRQ
                     VRRLMTGHAIPSISIEDLAKVLVPILPKKEQERIANSIASIQLMRKDALKAGEKVVME
                     THALISQTS"
     gene            2397..>3784
                     /gene="xerD"
                     /locus_tag="AC16GM_00535"
     CDS             2397..>3784
                     /gene="xerD"
                     /locus_tag="AC16GM_00535"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR2922196.1"
                     /translation="MNESIITYLTSLSIARGLAANTLAGYRNDLSAYQKYLAKKRISE
                     FERVTPEVVSGYVQHLSGLGRHPRSVARAVSSLRGFHRYLVLSRKAKVDPTAILEPLR
                     LPRKLPAVLDQNEVEKILEQPDNTPLGIRDGAILEYLYATGVRISELIGTRTRDLILE
                     AGFVRVFGKGKKERVVPIGSKAIKAVNDYLDDVRPTLANRKSADTVFLNARGGPLSRM
                     GAYNIILRYVRQAGIAKKVSPHTFRHSFATHLLEGGADLRVVQALLGHADISTTEVYT
                     HLDRAYLKEVHRTFHPRESNKKREDFRAAGIARGTVGGFRANRCACSPAPMFHCAFFS
                     LGSVTEAELRSTLGTEVVIFHRFHTVPQLAKQARRSNQDLIFLGSTGDLRSEITLVRQ
                     IKAQAILLVTPLVLYHPIPDRRMQLSALDSGADEFLSGSWDEELFGARLRMVVAKSHR
                     DVSVNPSSHLPGP"
BASE COUNT         1013 a          948 c          936 g          887 t
ORIGIN      
        1 tctttttgcc aacccacaac atcttgaatc ttcaggagac gaccctaatc atctccagca
       61 gaattacata atggggtttg tcttcaccga tgagcgaggc aactcaaact tgagtataac
      121 agaagaatgg tacaaagatt ttaatgaagt ctacgagacg ttcaatccgg aagattgcgt
      181 aagagaagaa gatatgcagg ttgataaccg atacgtagta ggaagtgaaa ctgattaata
      241 ggataaagta tggagacctc tgccaagtcc cgaagttgga aagtcgaaga acagcagttg
      301 ctgttttcgg aagatagcct tcttcctgac actttaccgc accttcgtga attacagccg
      361 tcacgcaacc ttgcggctat atttgatgag tgccacaact acatctacgc taatgaaggc
      421 ttgctaaagg acaaaatttt ccatgagata gtcaagttgc ttgtcatgaa gttatacgat
      481 gagcaaaacg tggctcaggg cattttacag ttcggggtaa cattgagcga atataggagc
      541 atccttgcta atacctctaa tactttcgag gagcgcctca acacgttgtt tgctattgtt
      601 aggagtaaat ataaggtttt ttttgcggat gaaatgctga agttaaggcc cctgacactg
      661 gcctatgtgg tcgggcgact acagaatatc aaccttacta aaacgcccgg agacattaaa
      721 ggggaagcct ttcaatcatt tgtataccgc catcaaaggg gcgaccgcgg tgagtttttc
      781 actcctcacc caatcgtcag acttgctgta gaaatgatag cacctaaacc tgatgaaaaa
      841 gttattgacc cggcttgtgg tagtggcgga tttcttgttc aaacgattgc ttacgtgcat
      901 cgaaactatc ccaacattga aaaggccgcc tacattcagg aacatatctc tgggattgaa
      961 ttcaaccccg atgtcgcttt gtcggcaatg attcgacttg cctttgaagg cggaactgga
     1021 acagaaatta cctgtacaaa ttctctcacg gaaaacgaga gactgaacaa ggctttcgat
     1081 gtagttctaa ctaatccccc ttttggcagt aagggcaaag tagaagatca gaaaatcctc
     1141 aagtcgtatg tcttagcgcg caggtggaat aagttatcaa ataacgattg ggaacctaca
     1201 aaaacggtac tgcccggaca atcgccagaa attctcttta ttgaaaaatg cctgaatttg
     1261 ctccgccccg ggggacgcat ggcgattgtt ctgcctgatg ggttacttca aaacatttct
     1321 aacagccaca taagattttg gattcgttcg caggccaaag tcttaggggt tgtatctatt
     1381 ccacaagaag cattcgttcc ctacggaaca ggcataaaaa catcgctttt actggtccaa
     1441 aaactgcctg ctagtagccg tggcgattgt tttatggctc gtattcaaaa gattggctat
     1501 gacgtgaaag gtcagttact atataggcgc gacgagtctg gaagattaat tagatcatca
     1561 tcgggtttac ctgttgttga tgatgacgta gaagaaatag caaccacatt caatgccttt
     1621 aaaaaagatg gggcactttg ccaaaacaga gaaaatatct ataccgcctc agaagaattt
     1681 cttaattcgc gtatggacgc tgaacactac caacctgatg acaaaaaact gattgagcat
     1741 ataagatcgc ttggcgcaaa gcctcttggc gaaatagcag atattctgac tgagaccgac
     1801 gatttcagac tagaaagcga tagcgagata agatatatcg ccatttcaga tgttgacgca
     1861 cgtacgatgc aggttgtttc gcagcaaatc ataaaagcgc atgaagcccc atccagggca
     1921 acttatcggc ttcgtaaagg cgacataatc acggctagct caggagcgag tacgggcaca
     1981 caacgtcaag ccacagcact tattaccgaa gatgaagacg gagcgatttg ttcaaacggt
     2041 tttgctgtaa tacggaacat tcaaggagta cagcccctat tcttgctggc gtatatgcgt
     2101 accgaatatt acttgcgaca ggttagaagg ctaatgacgg gtcatgccat tccttcaata
     2161 tcaatagagg atttagcgaa agttctagtg ccgattcttc ccaagaagga gcaggaaaga
     2221 atagctaact caattgcatc aattcagtta atgcgtaagg acgcgctgaa agcaggagaa
     2281 aaggttgtaa tggaaaccca cgctttgata agtcaaacga gttgataagt aaattcgcaa
     2341 atcaaaatag ggcggccccg aaggagcgcc tttttcttta tatttatcgg gatgccatga
     2401 acgaatccat catcacctac ctcacctccc tttccatcgc ccgcgggctg gcggcgaata
     2461 cgctggccgg ttaccggaac gacctgagcg cctaccagaa atatctcgcc aaaaaaagaa
     2521 taagcgaatt cgaacgggtg acgccggagg tggtctccgg ctacgtgcag catctttcgg
     2581 gtctgggccg ccatccccgc tcggtggcgc gggcggtttc cagtctgcgc gggtttcacc
     2641 ggtatttggt cctctcccgg aaagcgaaag ttgatcccac ggcgattctg gaaccgctcc
     2701 gcctgcctcg caaactgccg gcggtgctcg atcaaaacga agtggaaaaa atcttggaac
     2761 agccggacaa tacgccctta ggcatccgcg acggggcgat tttggaatac ctctacgcca
     2821 ccggagtaag gatttcggaa ctaatcggca cccgcaccag ggatttgatt ttagaagccg
     2881 gcttcgtccg cgtcttcggg aaagggaaaa aagaaagggt cgtgccgatc ggctccaaag
     2941 cgattaaggc agtgaacgac taccttgacg acgtccgccc cacgctcgcg aaccggaaat
     3001 cagcggatac cgtctttttg aacgcccggg gcgggccgct ctcccggatg ggggcgtaca
     3061 acatcattct gcgctacgtc cgccaggcgg gaattgccaa aaaggtctcc ccccacacct
     3121 tccggcactc ctttgccacc catttgctcg aaggcggcgc cgacctcagg gtcgtgcagg
     3181 cgctcctggg ccacgccgat atatctacta ccgaggtcta cacgcacctg gaccgtgcct
     3241 acttgaaaga ggtgcaccgg accttccacc cgcgggagtc caacaagaaa agagaggact
     3301 ttcgagcggc agggatagcc aggggcacgg tcggtgggtt cagagcgaac cggtgtgcgt
     3361 gtagccctgc gcctatgttc cattgtgcct ttttctctct gggatcggtc accgaagccg
     3421 aactgcgttc gactttaggg accgaagtcg tcatcttcca ccgcttccac accgtgcccc
     3481 agttggccaa gcaggcaagg cggtcgaatc aggatttgat cttcctcggc tcaaccggcg
     3541 acctgcgctc cgaaatcacc ctcgtccggc agattaaagc ccaggcgatc cttctggtga
     3601 cgcctttggt cctctaccac ccgatccccg accgccggat gcagttgtct gctttagatt
     3661 ccggggccga tgaatttctc tccggctcct gggacgagga actcttcggc gcgcgcctcc
     3721 ggatggtggt ggccaaaagc caccgcgacg tctcggtcaa tcccagttcg catctccccg
     3781 gccc
//
LOCUS       DBNGZD010000019         5197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_627137, whole genome shotgun sequence.
ACCESSION   DBNGZD010000019 DBNGZD010000000
VERSION     DBNGZD010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_627137"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="AC16GM_00540"
     CDS             <1..710
                     /locus_tag="AC16GM_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HIR2922197.1"
                     /translation="NPENARISSSEAQELLNLEPLAFQRLSKLIYKGNLYFSSGGSWS
                     PNGTWQFELTIEAIHYEDVQTIEDYLKAALYRKKSLIPKENPEIQLDLSSIKIEGIPP
                     QLMKFFQNLHPDIVNLCGKLVQNGHYSQAIFDAFKLVNKKVQEKSGKNDLDGASLMTT
                     VFSVKKPALQLNELKSKSDKDEQQGFMHIFQGAMIGIRNPKAHDIVEQKDPIKTLEYL
                     ALASLLLRRIEESEPRS"
     gene            721..1197
                     /locus_tag="AC16GM_00545"
     CDS             721..1197
                     /locus_tag="AC16GM_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922198.1"
                     /translation="MNLAHLHLILNHIPVVGVPLTLLILLWGELRKDAAVIRLGWLLL
                     VVLAVITIPAFLTGEPAEEIVEHLPGVEKGLINTHEDHATLALIVTLAGGLFSLLALV
                     FSSTRPLLHRFLRVTILVAALFSTAALLWTANAGGKIRHTEIRSGAAAVPTEKEDE"
     gene            1469..2593
                     /gene="hppD"
                     /locus_tag="AC16GM_00550"
     CDS             1469..2593
                     /gene="hppD"
                     /locus_tag="AC16GM_00550"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIR2922199.1"
                     /translation="MPQTTVQPKTAPAGVDFLPLEGIDYIEFYVGNAKQTAYYFRNAF
                     GFDITAYAGLETGVRDRASYVLEQGNIRFVFTAALGPDGRIADHLRLHGDGAKEVALR
                     VQDAESAFRETTKRGARAVQEPTEYRDDNGVLKKSAIATYGEVIHTFVERKNYAGVFA
                     PGYKKCLQRGKGLGLKEPDHVVGNVELGEMNEWVNFYRDKMGFSQMLHFDDQDISTAF
                     SALMSKVMQNGTGRIKFPINEPAEGKRKSQIQEYLDYYRSPGVQHIALSTDDIVATVT
                     RMRAAGVEFLYVPTTYYDLLQERVGKIDEPVEDLKRLGILVDRDDEGYLLQIFTKPVQ
                     DRPTLFFEVIQRKGSRGFGKGNFKALFESIEREQAQRGNL"
     gene            2650..3486
                     /locus_tag="AC16GM_00555"
     CDS             2650..3486
                     /locus_tag="AC16GM_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR2922200.1"
                     /translation="MKGGKVYTLFREFISLRQFLEQEQWLRLAREAEKRTTNGWEVEK
                     VLLAPPVPDPKTFRDFYAFEAHVKRARASRGLEMVPEWYELPVFYYSNPTVFVGPEEK
                     VKRPSYTQALDYELEIACIIGKEGKNIKASEAEAYIAGYTILNDWTARDAQRQEMKVG
                     MGPAKGKDFATALGPYLVTPDELQDLKNGDRYDLAMTARVNGKEYSRGNFKDIHWTFP
                     QMIERGSQEVTLFPGDVLGSGTVGTGCIMELGPDKYGWLKPGDVVELEIERLGILRNT
                     VV"
     gene            3553..4704
                     /locus_tag="AC16GM_00560"
     CDS             3553..4704
                     /locus_tag="AC16GM_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR2922201.1"
                     /translation="MPIYHSLGQIPHKRHTQFRQPDGALYSEQLMGSKGFSGMYSLLY
                     HSHPPTRVTKIGPVVENKIEFSDKKELKHYHFKTKEIEAGGDPITGRKVFMANEDVSL
                     AIARPTQKMSYFYRNGQADELYFIHEGSGVFQTIFGRLTFRPGDYVIIPRGTTYKIVS
                     NPGPNRFLVIESPSQIETPRRYRNEHGQLLEHSPYCERDIRRPEALETFDEKGEFEVK
                     IKARNRLTTYVFDFHPLDVVGWDGYLYPWIFNIEDFEPITGRIHQPPPVHQTFEGHNF
                     VVCSFCPRKFDYHPLAVPIPYNHSNVESDEMLYYCNAEFMSRKDIEYASITLHPSGIP
                     HGPHPGAVESALGKERTNELAVMMDTFRPLSVTKLAEKYEDDKYAYSWL"
     gene            complement(4741..>5197)
                     /locus_tag="AC16GM_00565"
     CDS             complement(4741..>5197)
                     /locus_tag="AC16GM_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR2922202.1"
                     /translation="RMSKFVQSEWSELTYHIIGSAMAVHRDIGPGHKEAIYQRALEQK
                     LREEKLTVSSQKSIEVTNDGTLVGLYFVDLLVEDKIVVELKALNRIENNHVQQVIAYL
                     AATGCPLGLLLNFGTGSLQTRRIFPPKNVSAYLINQQWLRAPEWMRPSE"
BASE COUNT         1289 a         1398 c         1391 g         1119 t
ORIGIN      
        1 ccaatcccga aaatgctcga atctcctcct cagaagccca agaattattg aacttagaac
       61 ccctcgcgtt ccaaagactt tcaaaattaa tttacaaggg taatctatat ttttcttctg
      121 gtggaagttg gtcaccgaat ggtacctggc aattcgaact tactattgaa gcaattcatt
      181 atgaggacgt gcagaccatc gaagattacc tcaaagcggc tctctaccga aaaaagtcac
      241 ttatcccaaa agaaaacccg gaaattcaac tcgacctttc ctctattaaa attgaaggaa
      301 ttccacccca acttatgaaa ttttttcaaa atctgcaccc tgatatagta aacctttgtg
      361 gaaaacttgt ccaaaacggt cactattccc aagcaatctt tgacgcattc aaattggtta
      421 acaaaaaagt ccaggaaaaa tcaggtaaaa atgacctaga tggtgcttca ctaatgacca
      481 ccgtcttttc ggtaaaaaag cccgcattac aactcaatga attgaaaagt aaatctgata
      541 aggacgaaca acagggattt atgcatattt tccaaggggc catgatcggg atacgaaatc
      601 ccaaggcaca cgacattgtg gagcaaaaag acccgattaa gacactggag tacttggctt
      661 tagccagttt gttgctacgc cgaatagagg aatcagaacc gcgttcgtag gcaacagaga
      721 atgaacctcg cccacctgca cttaatctta aaccacatcc cagtcgtcgg ggtgccgctc
      781 actcttttga ttttgctctg gggcgaactg cgcaaagatg cggcggtcat ccggctgggg
      841 tggctgttgc ttgtggttct ggcggtcatt acgataccgg cttttttgac aggtgagccc
      901 gctgaggaaa tcgtggagca cctgcccgga gtggaaaaag gcctgatcaa tactcacgaa
      961 gaccacgcca ccctggcctt gatcgtaacc ctggccggcg ggctgttctc gcttttggcg
     1021 ctggtattca gctccacccg gccgctgctc caccggtttc tccgcgtaac cattctcgtg
     1081 gccgcccttt tctccaccgc cgccctgctc tggacggcca acgccggcgg gaagattcgg
     1141 catacggaga ttcggtcagg ggcggcggca gttccaactg aaaaagaaga cgagtaaagt
     1201 gtttcgggtg cgttaagatg accccaccct tccctcccct acaggggagg gataacaggc
     1261 gcgaggccta gagattcgct cgggggcggc ggctcccaca tctgaaccag aggaagactg
     1321 aagcaacccc tccctcaccc tcccctcaaa ggggagggac atgtgcaggt gcgttacacc
     1381 tacccttgac ccttcctccc caacgggata aattaccgca aaaaggacag gttctctctc
     1441 ttatttacag aaagggtaag gaggttctat gccccagacc accgtgcaac ccaagaccgc
     1501 cccggcggga gttgatttcc tgcctttgga ggggattgac tacatcgaat tctacgtcgg
     1561 caatgccaag cagacggcct attacttccg caatgccttt ggattcgaca tcaccgccta
     1621 cgccggtttg gagaccggcg tgcgcgaccg ggcgtcctac gttcttgagc agggaaacat
     1681 tcgctttgtc tttaccgcgg ctctggggcc ggatggacgg attgccgacc acctgcgttt
     1741 gcacggcgac ggggcaaagg aggttgccct gcgagttcag gatgccgaaa gcgctttccg
     1801 cgaaactact aaacggggag cgcgggcggt gcaggagccg acggagtacc gtgatgataa
     1861 cggagtgctc aaaaaatcgg ccattgccac ttacggagag gtgattcata cctttgtcga
     1921 gcgcaaaaat tacgccggag ttttcgcacc gggatacaaa aagtgtctgc agcggggcaa
     1981 aggtcttgga ctaaaagaac ccgaccacgt ggtggggaac gtcgaattgg gcgagatgaa
     2041 tgaatgggtg aacttctacc gcgataagat gggcttttcg cagatgctcc actttgacga
     2101 tcaggacatt tcaaccgcct tctcggcctt gatgtcgaaa gtaatgcaga acggcaccgg
     2161 gcggatcaag ttccccatca acgaaccggc ggagggaaag cgcaagtcgc aaattcagga
     2221 atatttggac tactaccgct cccccggcgt ccagcacatc gccctctcca ccgacgacat
     2281 tgtggccacc gtcacgagga tgcgcgcggc cggagtggag ttcctctacg tgccgacgac
     2341 ctactacgat ttgctccaag agcgggtggg gaaaatcgac gagccggtgg aggatctaaa
     2401 acgtctgggg attttagtcg accgcgacga cgaaggatat ctcctgcaga ttttcaccaa
     2461 gccggtgcag gatagaccga cgcttttctt tgaggtgatt cagcgcaagg gctcgcgcgg
     2521 ctttggcaag ggaaatttca aggcgctgtt tgaatccatt gagcgggagc aggcccagcg
     2581 agggaacctg tagtgcggct ggtgaccttc taccctcccg acctgccgga gcagagcgcg
     2641 gtcggagtgg tgaagggcgg gaaagtctac acgcttttca gggagttcat ctccctgcgg
     2701 cagtttttag agcaggaaca atggctgcga ctggctcggg aggcggaaaa gcggactacc
     2761 aacggctggg aagtagaaaa agttctttta gcgccgccgg tgccggatcc gaagaccttt
     2821 cgggacttct acgccttcga ggcacacgtc aagcgcgccc gcgccagccg gggattggag
     2881 atggtgccgg agtggtacga gctcccggtt ttctactact cgaatccgac ggttttcgtc
     2941 gggccggagg aaaaagtcaa aaggccttcc tacactcagg cactcgatta cgagttggag
     3001 attgcctgca tcatcggcaa agaggggaaa aacatcaaag cgagcgaggc ggaggcttat
     3061 atcgccggct acacgatttt gaacgactgg acggcgcggg atgcgcaacg gcaggagatg
     3121 aaggtcggca tgggcccggc caaagggaaa gatttcgcta ccgcgttagg gccgtatctg
     3181 gtgacgccgg atgaattaca agatctcaaa aacggtgacc gctacgattt ggccatgacg
     3241 gcccgcgtga acggcaaaga atattcgcgg ggcaacttca aggacattca ctggactttt
     3301 ccgcagatga ttgagcgagg atcgcaagaa gtcaccctct ttcccggtga tgttttgggt
     3361 tcgggcaccg tcggcaccgg ctgtattatg gaattgggcc cggataagta cggctggctc
     3421 aagccggggg atgtggtgga actggaaatt gaaagattgg gaatcctcag aaataccgtt
     3481 gtctaaaaat cccaggaagc ggcacttcag tgccgcgtct tccattgggg atagttttga
     3541 aaggagcatt cgatgcccat ctaccattcc ttaggtcaaa taccccataa gcgacacacg
     3601 caattccgcc agcccgatgg agcgctctac tccgagcaac tgatgggttc caagggcttt
     3661 tcgggaatgt attcgctcct gtaccacagc catcccccca cgcgggtgac gaagatcggc
     3721 ccggtggtcg agaacaaaat cgaattcagc gacaaaaaag agctgaagca ctaccacttc
     3781 aaaaccaaag aaatcgaagc gggcggcgat cccattacgg gccgcaaggt gtttatggcg
     3841 aacgaagacg tttccctggc gatagcgcgt ccgacgcaaa agatgagtta tttctaccgc
     3901 aatgggcagg ccgacgagct ctacttcatt cacgaaggat cgggggtttt ccagaccatt
     3961 ttcggacggc tcaccttccg tccgggtgat tacgtcatca tcccgcgcgg cacgacctac
     4021 aaaatcgtct ccaaccccgg gccgaatcgg tttttagtca ttgaatcgcc cagccagatc
     4081 gaaacgccgc ggcgctaccg gaatgagcac gggcaactgt tggaacattc tccctactgc
     4141 gagcgggaca tccgccggcc ggaggcgctg gagacttttg acgaaaaagg cgagttcgaa
     4201 gtaaagatca aagcccgcaa ccggctgacg acctacgttt tcgatttcca ccccttagac
     4261 gtggtgggct gggacggcta cctctacccc tggattttca acattgagga ctttgagccg
     4321 atcaccggac gcatccacca gccgccgccg gtgcaccaga ctttcgaagg acacaacttc
     4381 gtggtctgct ccttctgccc gcgcaagttc gactaccacc cgctggcggt tcccattcct
     4441 tacaaccact ccaacgtgga gtcggacgag atgctctact actgcaacgc cgagttcatg
     4501 agccggaaag acatcgagta cgcgtccatc accctgcacc cctccggcat tccgcacggg
     4561 ccgcacccgg gcgcggtgga gtcggccctg ggcaaggaac gcaccaacga attagcggtg
     4621 atgatggata ccttccggcc gctgagcgtc acgaagctgg ccgaaaagta tgaggacgac
     4681 aagtatgcgt acagctggtt gtaacctgtc aacggatttt gaacggatta aaacggatta
     4741 ttattccgaa ggtcgcatcc actcaggagc gcgtagccat tgctgattga tcaaatacgc
     4801 agaaacattt ttaggcggga aaatacgtct cgtctgtaaa ctccctgttc cgaaattcaa
     4861 aagtagaccc aaaggacatc cagtcgcagc cagataagca attacttgct gaacgtgatt
     4921 gttttcgatt ctgtttaacg ccttcaattc cacaacgatc ttgtcctcaa cgaggagatc
     4981 gacaaagtaa aggcccacca gagttccgtc atttgtcacc tcaatgcttt tttgagaaga
     5041 cacagtaagt ttctcttcgc gcagtttttg ctctaacgct ctctggtaaa ttgcctcttt
     5101 gtgcccgggt ccgatgtcgc ggtggactgc catcgccgaa ccaataatat ggtaggtcaa
     5161 ttctgaccat tcgctctgta caaatttaga cattcga
//
LOCUS       DBNGZD010000020        10458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_627218, whole genome shotgun sequence.
ACCESSION   DBNGZD010000020 DBNGZD010000000
VERSION     DBNGZD010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_627218"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="AC16GM_00570"
     CDS             <1..908
                     /locus_tag="AC16GM_00570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2922203.1"
                     /translation="FGLRTAVLIGGASMTLQHQALERKPRVLIATPGRLLDHLEQRTV
                     ILSDIRILVLDEADRMLDMGFAPQINRILAALPRERQTMLFSASIPKEIVAIAQRHMQ
                     LPIHVEIAPSGTAAEKVTQEVFFVDREAKTRLLELLLQQYPGPALIFTRTKHGARNLA
                     RKVKARGHAAADIHSNLSLSQRRHALEGFKSGKYRILVATDIAARGIDVIGIELVVNY
                     DLPTNSEDYVHRIGRTGRAGRTGRAVSFATFDQRGEVKSIERLIRTALPVTALPALPP
                     EHPQPHPPHHTQAPRRPRPSHRRPH"
     gene            953..2197
                     /locus_tag="AC16GM_00575"
     CDS             953..2197
                     /locus_tag="AC16GM_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR2922204.1"
                     /translation="MQFALFFGILFFFAPSGRSAEPATLYVEARNDGRAGTGTEKNPY
                     RDLQTAIDRAKDGDQIMIRPGTYEAHSADYPESLCGNCQNHQTVVQATRGFLVQGKRV
                     HLVGSGPEKTTLVTKAGYGVLFDHSRGSVISGVRITGGVRDPDGNATDAGIVAKFSSI
                     TVAECEIGDNTNRIDTVVVGIGGVFGRENSELVIRDNLIKNNTWDGIALYRGATAFIT
                     DNVIDQGRGAGIGITWDASAIVLRNRISNYWKGIGTFGESRAVVRNNAVFDNLGWGIV
                     VTGSSFMEVTNNVIVRNGNCGLALWDSSASGKATNNVIIQNGWRKEWVCPCAGVVVWG
                     DSTKFPLSYNDVWNNVAGNWHEISDQTGQAGNLSADPFFLDKTDFHFQSASPLKDTGN
                     PYYTDPDGSRSDLGIYGGPGAR"
     gene            complement(2208..3470)
                     /locus_tag="AC16GM_00580"
     CDS             complement(2208..3470)
                     /locus_tag="AC16GM_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HIR2922205.1"
                     /translation="MVSDAKRIFYDFHLHSHYSRATSKDMEAKTLALWAERKGITVVG
                     TGDFTHPKYVAELKEQFVPSGHGLFRLKNSQSPVHFLLTVEVNNIYHQGGRLRKIHTL
                     LFAPSFEVVDKINARLARRGDLAVDGRPTFTFSAKELVKIVLDLSAECFIVPAHAWTP
                     WFSVFGSNSGFDSLEECFGEETKHIHAIETGLSSDAAMNWRLSALDRVAVISNSDAHS
                     PAKLGREATVLSCNLDYQEIIDAIKTKDPKRFLYTIEFFPEEGKYHYDGHRDCRINWH
                     PNESKAHHNTCPACGKGLTLGVLHRVEQLADRPDGYQPPGAIPQKHLIPLAEIIADAF
                     GQGVDTKAVQEEYLRLIRLLGSEFEILLDRTGEEIARVATPEVTEGIRRVRAGKVSIV
                     PGHDGVYGKISLFAEGEKIETQPQLSLL"
     gene            3496..4392
                     /locus_tag="AC16GM_00585"
     CDS             3496..4392
                     /locus_tag="AC16GM_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922206.1"
                     /translation="MVQSIATADQRQVSFRQEISGSQLPIRQRMPVYGYFGLGVIVLA
                     EILLFAGLKLVGLFFTPVAWSGYILLVDALVFRAQSSSWLTTRRKEFLLLLPISIILW
                     LIFEGYNQFIDNWHYVGLPENLFVRVFGYAWSFATIWPAILETNEFLLVNGVFAKTRV
                     RPFLFSRPALNLLLVVGPAFLAIPILFPSRWWAVLVWTGFVFLFDPLNFRRGLPSLIT
                     ELRQGRPQLLLTLMFSGLICGFLWEFWNYWAAAKWFYTVPLWGKVKLFEMPVVGYLGF
                     PAFALEVFVMYHFVRGFLLRKF"
     gene            complement(4406..5758)
                     /locus_tag="AC16GM_00590"
     CDS             complement(4406..5758)
                     /locus_tag="AC16GM_00590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2922207.1"
                     /translation="MLGQELIFEQLRKIIKQSPADQTEAVFSGSTVGTTRLVNSAIAE
                     SRSKESSEVYLRVVIGKQIGVVACDEFEENTLTSALKEALTVAKLHKEHPRPVVLPGA
                     QSYPTLNIYSTETASYPQKQCDQQVATLLQKTKQSHLALSGFFSTAQGEIAVLNSNGL
                     SAYQPFTSASTTLTLGQDGHVGTVNDLARDVGQINLAALWNQAYERAQIPDKTRTIEP
                     GNYTVVFEPRAVAHLLEWVNYIGFRSAAYQDKISFLSERLGKTVFGENISIYDDAGDT
                     SSLAFPFDFEGIPKRKTPLIERGVAKSIVYDTTSAAQDGVKTTGHAIVTNVAYNGAMA
                     VNLFLEPGKISKSELLRSVEQGLIIRRFYSLNGLLDPRNALVLGTTREGCLWLEKGKV
                     RANVPDVRINASLFDIFSNVVALSRETERVPYWWDGIGCITVPTIVVKNFPIAGLAKS
                     "
     gene            complement(5774..6691)
                     /locus_tag="AC16GM_00595"
     CDS             complement(5774..6691)
                     /locus_tag="AC16GM_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR2922208.1"
                     /translation="MPDKVTSLSEVIRTHVPDGSAVVMCTGMEQNIPFAAGHEIIRQK
                     KRDLTLIGPISDILFDQLIGAGVAKKVMAAWVGNVISGSAYNFRRAVEEKIPYPIEVV
                     DYSNFTVTLALHAGALGVPFLPTFSTLGSDVQKNNPHLKEFIEPFGQEKMIAVQALEP
                     EVAIIHVQRADPEGNCHLWGGLGITLDAARASKKVIVTAEEIVAPEVIASDPNRTLIP
                     GFLVTAVAEVPFGAHPSPVQGYYNRDHQTYVDYHEQTKSRTGFKKWLDKWAFGIQDQK
                     DYCNKLGDVRLKSLEVKTHAYAAAVDYGY"
     gene            complement(6784..7317)
                     /locus_tag="AC16GM_00600"
     CDS             complement(6784..7317)
                     /locus_tag="AC16GM_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR2922209.1"
                     /translation="MKKFMLLVSLFTLSLFFFGCAKPIDPEKIKANTRELDDKWKAAF
                     AKEDINGVMGCYWNSPEVIFYPPDAMGEKGYEAIKAGYEKFFAENEVKSAELSNVSSM
                     ALGDYGLDWGNWSITIGTPDGRETKLEGRFTNVSGMRDGKWVWIHDHASAPLPPPPTP
                     EEMQKMMKEMMKKPMKK"
     gene            complement(7445..7906)
                     /locus_tag="AC16GM_00605"
     CDS             complement(7445..7906)
                     /locus_tag="AC16GM_00605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR2922210.1"
                     /translation="MNTESPPIKIWPPVVYLSTLAAGVILHLFTPLRIFPQAWIGHVI
                     GWPVLVIGAVLVAWGKRTMDRAGVNPRFKPVNTLVSTGPFTFTRNPLYLGTNLMFAGI
                     ALVVNALWILVFLLPTVIYLHLSVIPREEGYLERLFSDEYRQYRERVRRWL"
     gene            complement(8052..8627)
                     /locus_tag="AC16GM_00610"
     CDS             complement(8052..8627)
                     /locus_tag="AC16GM_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR2922211.1"
                     /translation="MNNYSRENALALVREHTKNENLVKHMLAVEAAMRFYARKYGEDE
                     NKWGTVGLLHDFDYEKFPNQEHNPTAGHPTEGSKILREKGYPEEIIRAILSHADYTGI
                     PRESVMEKALFACDELTGFVVACALVRPSKTLAEVEVNSVRKKMKDKAFARQVSREDI
                     TKGAEELGLPLEEHIGNVLEAMKGIAKELGL"
     gene            complement(8620..9573)
                     /locus_tag="AC16GM_00615"
     CDS             complement(8620..9573)
                     /locus_tag="AC16GM_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922212.1"
                     /translation="MANVFFDANIYFDLFKRKEFTPYFDVLSEVVKKNRLRIHFTSVN
                     FDEFLICLNQNNFEKVREDFRKASILTNSGNCVEFPWWHVGRAVAIEFGFPVPELAFE
                     YIGLCRKISLAASYNEVSAEIEREKSHLQEFKQNQVASREQILVLARHRIRDSKISAN
                     ELFSPPLSDNFLTPIREAIWRALTKALVLRFKFPEECQQITYQQAVLRIPSVALYCDA
                     HIGYLYRQLVEKVKTRGGDYFDMEQVVYLDIMDYWISNDKGAWANLEKFSNKEILNRI
                     LTLEQFIARLLNDDLRPCALSRTKQLYYTQTEQGIVLHEHE"
     gene            complement(9579..>10458)
                     /locus_tag="AC16GM_00620"
     CDS             complement(9579..>10458)
                     /locus_tag="AC16GM_00620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR2922213.1"
                     /translation="SPDLAGEKTKTPKPAIPTRPLGKTGYEATIFALGGQAKIEERGE
                     RDSAVEIINRALDLGVNYFDTAPAYGPSQDYFGAVLKTRRKEVFLASKTHNRSRDGSL
                     RLLEDSLTRLNTDHLDLWQLHNINDQEDLDQIFSKNGAIHALEIAREQKMVRFFGITG
                     HYDPRLLVQGIERYPFDCTLCALNAADRHHLSFIETLLPMATKKQMGIIGMKVPARGS
                     IFRKDGLATMKQAMDYVLTLPVSTIIVGCSTVAELEENVRIAKEFKPLSEREMAQLED
                     LTRPYARQASWFKDWA"
BASE COUNT         2188 a         2852 c         2857 g         2561 t
ORIGIN      
        1 ctttcggcct gcggacggcg gtgctgatcg gcggagcgtc gatgacgctc cagcaccagg
       61 ccctggagcg aaaaccgcgg gtcctcatcg ccaccccggg acgcttgctg gatcatctcg
      121 aacaacgaac ggtcattctc tccgacatac ggattctggt cttggacgag gccgaccgaa
      181 tgctcgatat gggttttgcg ccgcagatta ataggatttt ggccgcactg ccgcgggaac
      241 gtcaaaccat gcttttctcg gccagcatac ccaaagagat tgtggccatt gcccagcggc
      301 acatgcaact tccgatccac gtcgagattg ccccttcggg aacggctgcg gaaaaagtca
      361 ctcaggaggt atttttcgtc gaccgggaag ccaagactcg cttgctggag ctcctgctgc
      421 aacaatatcc cggcccggcc ttaattttta cccggacgaa gcacggggcg cggaatctcg
      481 ctcgcaaagt aaaggcccgg ggacacgccg cggcggacat ccactctaac ctgagcttgt
      541 cgcagcgccg ccatgccttg gaaggattca agtccggcaa gtaccgcatt ctggtagcca
      601 ccgatattgc ggcccggggt atcgacgtca tcgggatcga gttggtggtc aactatgatc
      661 tgccgaccaa ttccgaagac tatgtgcacc gcattggtcg aaccgggcgg gcgggccgaa
      721 ccggccgggc cgtctctttt gccaccttcg atcaacgggg ggaggtcaag agcatcgagc
      781 ggttgatcag aaccgcactg cccgtgacgg cgttgcccgc cttgcctccc gagcaccccc
      841 agccccaccc cccgcaccac acccaagcgc cccggcgccc gcgaccatcg caccggcggc
      901 cgcactaaag aaagagttca tgattgcctc tcagacaatc agtcgtggtg cagtgcagtt
      961 cgcgctcttc tttgggattc ttttcttttt tgctccttcg ggtcggtctg ctgaaccagc
     1021 cacactctac gtcgaggcgc gcaatgatgg gagagccggt accgggactg agaaaaatcc
     1081 ctaccgcgat ctccagaccg ctattgaccg agccaaggat ggtgaccaga ttatgattcg
     1141 gcccggcacg tatgaagcgc attcggctga ttacccggaa agtctctgcg gtaactgcca
     1201 gaatcaccag accgtcgttc aagccacccg gggatttttg gttcagggga agcgggtgca
     1261 cttagtcggc agcgggccgg aaaaaacgac cctggtgacc aaagccggat atggcgttct
     1321 cttcgaccat agccgcgggt cggtcatctc cggcgtgcga atcacgggcg gagtgcgcga
     1381 tccggacggc aatgccaccg acgccggaat cgttgccaaa ttttcaagca tcacagtggc
     1441 ggagtgtgaa atcggcgaca ataccaaccg catcgacacg gtcgtcgtcg ggatcggcgg
     1501 cgtctttggc cgggagaatt ccgaactggt gatccgggac aatctcatca agaacaacac
     1561 ctgggacggc attgcgctct accgcggggc gaccgccttc atcaccgaca acgtcattga
     1621 ccagggtcgg ggcgccggga tcggcatcac ctgggatgcc tcggccatcg tcctgcgcaa
     1681 ccggatttcc aactactgga aagggatcgg cacctttggt gagtcgcgcg ccgtggtgcg
     1741 caacaatgcg gtcttcgata acctgggctg gggcatcgtg gtcaccggat cgtcctttat
     1801 ggaagtgacc aacaacgtca tcgtgcgcaa cggcaactgc ggcctggcgc tgtgggattc
     1861 ttcggcttcc gggaaagcga ccaacaacgt catcatacag aacggctggc gcaaagagtg
     1921 ggtttgcccc tgcgcggggg tggtggtctg gggcgactcg actaaattcc ctctctcgta
     1981 caacgatgtc tggaacaacg tcgccgggaa ctggcacgaa atttccgacc agaccgggca
     2041 ggcgggtaac ctttcagccg accccttttt tctggataag accgattttc actttcaatc
     2101 agcatcgccg ctaaaggata ccgggaatcc ttactacacc gacccggatg ggtcgcgaag
     2161 cgatctggga atttacggcg ggccgggagc gcggtagaac tcgtctttta gagcagactc
     2221 agctgcggct gggtttcgat tttttcacct tcagcgaata gggaaatttt cccgtagacg
     2281 ccgtcgtggc ccggcactat tgagactttc cctgcccgga cccgtctgat tccttcggtc
     2341 acttccggtg tggcgactct ggcgatttcc tctcccgttc ggtcgaggag aatttcaaac
     2401 tcgctcccca gcaggcgaat taaccgcagg tactcctcct gcaccgcttt ggtatcgacc
     2461 ccctggccga aggcatcggc aatgatttcg gccaatggga tcaaatgttt ttgtggaatg
     2521 gcccctggag gctggtaccc atccggacgg tcggctaact gctccacccg atggagcaca
     2581 cccagagtca acccctttcc gcacgccggg caggtgttgt ggtgcgcctt gctttcattt
     2641 gggtgccagt tgatccggca gtcgcggtgg ccgtcgtagt ggtacttccc ttcctcgggg
     2701 aaaaactcga tggtgtagag gaagcgtttc gggtccttcg ttttgatggc gtcaatgatt
     2761 tcttggtagt cgagattgca ggagagtacg gtggcttccc ggccaagttt ggcgggcgag
     2821 tgcgcatcgg agttggaaat gacagccacc cggtcgagcg ccgagagccg ccagttcatt
     2881 gccgcgtctg acgagagccc ggtctcaatg gcgtgaatgt gcttggtttc ttcgccgaaa
     2941 cattcttcca acgaatcgaa gccggaattg gagccgaaga ccgaaaacca gggcgtccag
     3001 gcatgggccg ggacgatgaa acattctgct gacagatcga ggacgatttt cactaattct
     3061 ttggccgaga aggtaaaggt cggccggcca tcgaccgcca gatcaccccg ccgggctaac
     3121 cgggcgttga ttttgtccac gacctcaaac gaaggggcaa agaggagagt gtgaattttc
     3181 cggagccgac ctccttggtg gtaaatgtta ttcacctcta ccgtgagcag gaagtgcacc
     3241 ggcgattggc tgttttttaa gcgaaatagc ccatggccgg aggggacgaa ctgttccttt
     3301 agttccgcta cgtactttgg atgggtgaaa tcgccggtgc cgacaacggt gatgcctttc
     3361 cgttccgccc agagcgccag cgttttggct tccatatcct tgctcgtggc acggctgtag
     3421 tgggagtgca ggtggaaatc gtaaaatata cgctttgcat cggacaccat cggcgaaata
     3481 tattgcctga aatgtatggt gcaatcaatc gcgactgctg accagcgcca agtaagtttc
     3541 cgtcaggaga tatccgggag tcagcttccc attcgccagc gtatgccggt ctacggctac
     3601 ttcgggctgg gtgttatcgt cctggctgag attttgctgt ttgcgggcct gaaactggtc
     3661 ggcctctttt tcacacccgt tgcttggagc gggtacatcc tgttggttga tgcgctggtg
     3721 ttcagagcgc agagcagctc ctggctgact acccggcgta aagaattcct gttgttgctc
     3781 cccatctcga taattttgtg gctcattttt gaaggataca accaattcat cgacaactgg
     3841 cactatgtgg gcttgcccga aaatctcttt gtgcgtgtgt tcggctacgc ctggtctttc
     3901 gccaccatct ggccggcgat tctggaaacc aatgaattcc ttttggtcaa tggagttttt
     3961 gcgaaaacgc gcgtccggcc ttttctcttc tcgcgtccgg cgctaaattt acttctggtt
     4021 gtcggtcccg cctttctcgc tatacccatt ctgtttccct cgcgttggtg ggccgttctg
     4081 gtctggaccg gtttcgtctt cctcttcgat ccgctcaact tccgccgcgg cttgccctct
     4141 ctcatcacgg agctgcgaca gggcaggcct cagttgcttt tgactttgat gttttcggga
     4201 ttgatctgcg gttttctctg ggaattctgg aactattggg ccgcggcgaa gtggttctac
     4261 acggtgcccc tctggggcaa ggtgaaactc ttcgaaatgc cggtggtggg gtatctgggt
     4321 tttccggcct ttgccctaga ggtgttcgtt atgtaccact tcgtgcgggg gttcctgctc
     4381 agaaaattct aatttttaga agaaattagg atttggccag accggcgatg gggaagtttt
     4441 tcaccacgat ggtcggtacg gtgatgcagc cgatcccatc ccaccagtag ggtacccgct
     4501 cggtttcccg cgaaagggca accacgttgg agaagatgtc aaacaaactc gcgttgattc
     4561 gcacgtccgg cacgttcgcc cttactttcc ccttttccag ccacaggcag ccttcccggg
     4621 tcgttccaag cacgagcgcg ttgcgcggat cgagcaagcc gttgagtgaa tagaaacgcc
     4681 ggataatcag gccttgctct acggaacgta aaagttcgct tttggagatt ttgcccggtt
     4741 ccaggaaaag gttgaccgcc atggcgccgt tgtaggccac gttggtgacg atggcgtggc
     4801 cggtggtttt gacgccgtcc tgtgccgcgc tagtggtgtc gtagacgatg ctcttggcca
     4861 ccccccgctc gatgagcggc gttttccgct tgggaattcc ctcaaaatcg aatggaaaag
     4921 ccagcgacga ggtatcacct gcgtcatcgt agatagaaat gttttctccg aaaaccgtct
     4981 ttcctaaacg ctccgaaagg aagcttattt tatcctgata cgccgccgag cggaagccga
     5041 tgtagttcac ccactccaga aggtgcgcta cggcgcgcgg ctcgaacacc accgtgtaat
     5101 ttccgggttc aatcgttctg gtcttgtccg ggatttgagc gcgctcgtat gcttgattcc
     5161 atagggcagc gaggttaatc tgcccgacgt ctcgcgctag gtcatttacc gtgccgacgt
     5221 gaccatcttg gcccaacgta agggtagtcg aggccgaggt aaacggctgg taggcggaaa
     5281 ggccgttgga gttgagcacc gcaatttcgc cttgggcggt ggagaaaaag ccggacaggg
     5341 caaggtgtga ctgcttggtt ttttgcaaaa gggtcgccac ttgctggtcg cactgttttt
     5401 gcggatagga ggcggtctcg gtagagtaaa tgttcagagt cggatacgat tgcgctccgg
     5461 gcagcacgac gggccggggg tgttccttgt ggagtttggc tacggtcagc gcctctttta
     5521 gggcggaggt cagggtgttt tcttcaaatt catcgcaggc taccaccccg atttgcttgc
     5581 ctatcactac ccgcaggtag acttcacttg actctttgga gcggctctcc gcgatggccg
     5641 agttcacaag gcgggtggtg cccaccgtac tgccgctgaa aaccgcctcg gtctggtcag
     5701 ccggcgattg cttaataatt tttctcagct gttcaaaaat cagttcttga ccgagcatag
     5761 ggattcattt ctctcagtaa ccgtaatcca ctgcggcagc gtaggcgtgg gttttgacct
     5821 caagcgattt cagccgcaca tctcccagtt tattacaata gtctttttga tcttggatgc
     5881 caaacgccca tttgtccagc cattttttaa aacccgtacg cgattttgtc tgttcatggt
     5941 aatcgacata ggtctggtgg tcgcggttgt agtagccctg caccggtgac gggtgcgctc
     6001 cgaacggcac ttccgccact gcggtgacaa ggaatccggg aatcaacgta cggtttgggt
     6061 cgctggcgat gacttccggg gctacaattt cttcggcggt aacgatcact ttcttactcg
     6121 cccgcgctgc atcgagcgtg attccaagcc cgccccagag atggcagttg ccttccggat
     6181 cggcccgctg gacgtggatg atcgccacct ccggctccag cgcctgcacc gcaatcatct
     6241 tctcctgacc gaatggctcg atgaattctt tcaagtgcgg attatttttt tgcacgtccg
     6301 agccgagcgt cgaaaacgtg ggcaggaagg ggacgcccag tgctccggcg tggagcgcca
     6361 gtgtcaccgt gaaattggag taatcgacca cttcaattgg gtacgggatt ttctcttcca
     6421 ccgcccggcg aaagttgtag gccgagccgg aaattacatt ccccacccag gcggccatga
     6481 cttttttcgc cacacccgct ccgattaact ggtcaaacag aatgtccgaa attgggccga
     6541 tcaaggtcag gtcgcgtttt ttctggcgga taatttcgtg cccggcggcg aagggaatgt
     6601 tctgctccat accggtgcac atgacgaccg cagagccgtc ggggacgtgg gttctaatta
     6661 cttcggatag agaggttact ttatcaggca tcaggaaatc atttttttca ggcgggacta
     6721 agagtcccac ctacgcaact ttgtcggcca caaacaaaaa tgcccctcgc ggggcattcg
     6781 ctattacttc ttcattggtt tcttcatcat ttctttcatc atcttctgca tctcttccgg
     6841 tgtgggaggc ggcggcagag gggcagaggc atggtcgtgg atccaaaccc atttaccatc
     6901 tctcataccg gaaacgttag taaatcttcc ttctaatttc gtctcccgtc cgtcgggggt
     6961 gccgatagta atggaccagt tcccccagtc taagccataa tctcctaaag ccatggaaga
     7021 aacattgctc aactccgcac tcttaacttc attttccgca aaaaactttt catatccggc
     7081 tttaatcgct tcatatcctt tttctcccat tgcatctgga gggtaaaaaa ttacctcagg
     7141 agaattccag taacagccca tcacgccgtt gatgtcttct ttcgcaaaag cggccttcca
     7201 tttatcgtct aattctctgg tattggcttt tattttttcc gggtcaatgg gttttgcgca
     7261 cccgaagaag aaaagcgaca aggtgaagag cgaaacgagc aacataaact ttttcactgt
     7321 accctcctct ggtttgtggt taccgtttca acgccgggtg tgaaataatg tcaccgtaaa
     7381 gagaagtcaa gcgatcgtaa aaattttcag gcgggactaa aagtgccgcc tactggtctt
     7441 ctggtcacaa ccagcggcgg actctctccc ggtactggcg gtactcatca gagaaaagcc
     7501 gctccagata accttcttcc cggggaatga cgctcaagtg caggtagata accgtcggga
     7561 gcaaaaagac gagaatccag agcgcattca cgaccagagc gatccccgca aacatcaggt
     7621 tggtgccgag gtaaagcgga ttgcgggtaa aggtgaaggg cccggtggac accagcgtat
     7681 tgaccggttt aaacctgggg ttaactccgg cgcggtccat cgtccgcttg ccccacgcca
     7741 caaggacagc accgatcacc agcaccggcc agccgatgac gtgccctatc cacgcttgcg
     7801 gaaaaatccg gagcggcgta aagaggtgga gaattacgcc tgccgccagc gtcgagagat
     7861 aaacgaccgg cggccagatt ttaattggag gagattcggt gttcatgcct tttggtagac
     7921 cggtcggtac gacccgtatg ggctcgtccc tgagccggtc ataatgtagt tttcctttca
     7981 gcggggggcg ggactaagag tctcggtacg agtcgtatcg accctccaac gcgagagaag
     8041 aagggtttgc atcacaaccc caattctttg gcaattccct tcatcgcttc cagcacgttg
     8101 cctatgtgct cttccagagg tagccccaac tcctccgcgc ctttggtaat gtcctcccgg
     8161 ctcacctgcc gggcgaaggc cttgtccttc attttcttcc gcaccgagtt cacctccacc
     8221 tccgctaagg tcttgctggg ccgtaccagc gcgcaggcga cgacgaagcc ggttagttca
     8281 tcgcaggcga agagcgcttt ttccataaca ctttcgcggg ggatgccggt gtagtcggcg
     8341 tgggagagga tagcccgaat gatttcctcc gggtagccct tttcccgcaa aatctttgag
     8401 ccctcggtcg gatgccccgc tgttggattg tgctcctggt tggggaattt ctcgtagtca
     8461 aagtcgtgca ggaggccgac tgtgccccac ttattttcat cctcgccgta ctttcgggcg
     8521 tagaagcgca tcgccgcttc cacggcgagc atatgcttga ccaaattttc gtttttggtg
     8581 tgctcgcgga ctaaggcgag ggcattttct ctggagtaat tattcatgtt cgtgcagaac
     8641 aattccttgt tcagtttgag tgtaatataa ttgcttcgtt cgagacaagg cgcaggggcg
     8701 caaatcatca ttcagtagtc tcgctatgaa ttgctctaac gttagaatac gattcaggat
     8761 ttctttgttg gaaaattttt ctaaatttgc ccatgcacct ttatcatttg aaatccaata
     8821 atccatgatg tctaagtaga cgacctgttc catatcaaag tagtcaccac ctcttgtttt
     8881 tactttttca accaattgac gatacaaata ccctatgtgg gcatcgcaat acaaagcgac
     8941 cgagggaata cgcaaaacag cctgctggta tgtgatttgc tggcactctt ctggaaactt
     9001 aaaacgtagc actaatgctt tagtcaatgc tcgccaaata gcttctctta tcggagttag
     9061 aaaattatcc gaaagcggtg gagagaaaag ttcgttagct gatattttcg agtccctgat
     9121 tcggtggcgg gccagaacca gaatctgttc gcgtgaggct acctgattct gcttaaattc
     9181 ctgtaggtgt gacttctctc gttctatttc cgcactgact tcattataac tagcggctaa
     9241 tgaaattttt cgacataggc cgatatattc aaatgctaat tccggtactg gaaagccaaa
     9301 ttctatcgcg actgccctcc caacatgcca ccatggaaat tcaacgcagt ttccactgtt
     9361 cgtaagaatg gacgcttttc taaagtcctc tcggactttt tcgaaattgt tttgattaag
     9421 gcaaattaga aattcatcaa agtttactga agtaaaatga atcctgagac ggttcttttt
     9481 tactacttca gacaaaacat caaaataggg ggtgaactcc ttccttttga agagatcgaa
     9541 atagatattg gcatcgaaaa agacattggc cataaaggct acgcccaatc cttaaaccag
     9601 ctcgcctgcc ttgcgtacgg gcgtgtcaaa tcttccaact gcgccatttc gcgttccgaa
     9661 agcggcttga attctttcgc aattcgcacg ttctcttcca gttcggctac ggtcgagcac
     9721 cccacgataa tcgtgctgac cggcaaagtc aaaacatagt ccatcgcctg tttcatcgtg
     9781 gccaggccgt ccttgcggaa gatgctcccc cgcgccggca ccttcatgcc gataataccc
     9841 atttgctttt tcgtcgccat cggcaggagc gtttcgatga acgagaggtg gtgccggtcg
     9901 gcggcgttca gagcgcagag ggtgcagtca aagggatacc gctcgatccc ctgaactaaa
     9961 agccgtgggt cgtagtgccc ggtgatgcca aagaagcgga ccatcttttg ttcgcgggct
    10021 atttcgagag cgtgaatggc gccattcttg gagaaaatct ggtcgaggtc ttcctgatca
    10081 ttgatgttat gcaactgcca gaggtcgagg tggtcggtgt tcagccgcgt gagactatcc
    10141 tccaaaaggc gcagggagcc gtcccgggag cggttgtggg ttttggaggc cagaaagact
    10201 tctttccgcc gggtcttgag cactgcgccg aagtagtcct gcgacggacc ataggccggg
    10261 gccgtatcga agtagttcac gcctaaatcg agcgcacggt tgataatctc gacagcgcta
    10321 tcccgctcgc cccgttcttc gatcttcgcc tgcccaccca aagcgaaaat cgtcgcctcg
    10381 tagccggttt ttcccagggg acgtgtgggg atggcgggtt tgggagtttt tgttttttct
    10441 ccggccagat ccggactc
//
LOCUS       DBNGZD010000021         3500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_836574, whole genome shotgun sequence.
ACCESSION   DBNGZD010000021 DBNGZD010000000
VERSION     DBNGZD010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_836574"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            616..1413
                     /locus_tag="AC16GM_00625"
     CDS             616..1413
                     /locus_tag="AC16GM_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR2922214.1"
                     /translation="MPTAQILTEREQKKYLALDQEAKKLLEFKKENIRKNHLTPCPQC
                     ATWINFKAKKCPQCTSDIAGHMQKVREQLKRLDEISAELYELHKKKMELYHEGSAYKP
                     IWERVQAFFSDQQLLNDFKIVLPTLVSFFALLFFLKSQDFGILFWLAALGGGTGVFFL
                     FKKWNLRKHVVLDFYRMVLIIGLVILLTATLFSGATIWPGKSVSGKTVVVTAPTANLR
                     ESPNTSADVVATVQEGERVTVTGKRGSWYRVKAGNRKGWILSDLVST"
     gene            1586..1875
                     /locus_tag="AC16GM_00630"
                     /pseudo
     CDS             1586..1875
                     /locus_tag="AC16GM_00630"
                     /inference="COORDINATES: protein motif:HMM:NF039191.5"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
     gene            2305..2946
                     /gene="tmk"
                     /locus_tag="AC16GM_00635"
     CDS             2305..2946
                     /gene="tmk"
                     /locus_tag="AC16GM_00635"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR2922215.1"
                     /translation="MRKKRGFFITFEGIDGSGKTTQAKKLVRYLKRKRIPVLFLREPG
                     GTNVAEKLRQIVLKATGERIAPAAELFLYLAARAQIVQNRIAEARARGIMVICDRFSD
                     STVAYQAYGRGWNQKLIAELNAMASNSTLPELTLLLDLPVRVGLRRKKTALGGFPYKD
                     RLESEGKSFHRRVRRGYLKTARAEPERIVVIDGTGSPETVWEEIRRVVDKKIA"
     gene            2963..>3500
                     /locus_tag="AC16GM_00640"
     CDS             2963..>3500
                     /locus_tag="AC16GM_00640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR2922216.1"
                     /translation="MTPIERKQLARGIFFGFLSVVILAAALAYGLGGDLIGRSRLVET
                     IASRIESDYVDYVPADSLIDSGIAGMFNRLDPYSEYLPPANYRSLLEDTQGHFGGLGI
                     EIVVRNNAILVIAPLEGTPAARAGLLPGDRIVKIDGRTAAGMTGEEAVATLRGPQGSA
                     VTIEVKREGLSDPLVLTLK"
BASE COUNT          806 a          927 c         1040 g          727 t
ORIGIN      
        1 ttacgtagac gacggcttgg taggccgccg aggttggtac ggtgcgactc tatcgagaaa
       61 cctcggctac gaaatgccct taaagcattc ctttttttat cagggcaatt catgaattgc
      121 cccgacaacc ccaacgggga caagtcccta tgctacagat acaaaaatct cctatcgcgg
      181 cgcaggttgg tatcccgaat ggaacctgcg ccgttttgat tttgggaaat gtacagggcg
      241 acgagacgtc gcccctacaa aggcctgggc agacacggtt cgatcccgcc gttggcggga
      301 ctcaccgcag gcgggggtct gcccctacat caaatcggcg cgggatacgc aaatcattcc
      361 gcgctacgat agttcgttcc gaggctggtc tgaaacgact ctacgagaag cctcggctat
      421 ggaagtaaag agtatctttg ttccataggt caggcttcca gccccgcctc tggcgggact
      481 tccagcctga cgggagaagt ccaatgtttt gcgcgggtgg acgtagacat ccacccatac
      541 gtcgtggtac agaataaatc caggcgtaaa ctccccccca tttccgccga taatagggta
      601 gacggagggt ataccatgcc gactgcccag atattgaccg aacgggaaca gaagaagtac
      661 ttggcgctcg accaggaagc caaaaaactc ttagagttca aaaaagagaa tatccggaag
      721 aaccacctga cgccctgccc acagtgcgcc acctggatca acttcaaggc caagaagtgc
      781 ccgcagtgca cgtcggacat tgctggtcat atgcagaagg tccgggagca gttgaagcgg
      841 ctggatgaaa tcagcgcgga actgtacgaa ctgcacaaga agaaaatgga gttgtaccac
      901 gaagggtcgg cgtacaagcc gatctgggag cgggtgcaag cctttttctc cgaccagcag
      961 ttgctgaatg atttcaaaat cgtcctccct accctcgtca gtttctttgc gctcctgttc
     1021 ttcctcaaaa gccaggattt cgggatactc ttctggctgg cggccttagg cgggggcacg
     1081 ggcgtctttt tcctcttcaa gaagtggaat ctgcggaagc atgtcgtcct cgacttctac
     1141 cgcatggtgc tgattatcgg gttggtcatc ctcctcaccg caacgctctt ctccggagcc
     1201 acgatctggc cgggaaaatc ggtcagcggg aagaccgttg tggtcaccgc acccacggcc
     1261 aatctgcggg aaagccccaa taccagcgcc gacgtggtgg ccaccgtcca ggagggcgag
     1321 cgggtgaccg tcaccggcaa gcgcggctcc tggtacaggg tgaaggccgg caaccgcaag
     1381 ggctggattc tctctgatct ggtgagcacg taagcgacct gagtcctccc gcagactgtt
     1441 ttctttaggc cctcctttca tccggcataa agacttttcg tttttttctt tgcgtcagta
     1501 tattcccgca acagctctac ccggtgcttg agataacgtg attcacctgc tccgagaacg
     1561 cgccacgcct gagcaactgg aagatatgct ggcaacgctg gagacatacg tgaaagtggc
     1621 ggtagatgtc cggcgggatg ttctggcagg cggggggatt ttgcacgccg actgtgggga
     1681 agtattgttg caggatggaa gcaaagcgga gatatttggg gtgcagactg gtaccctgag
     1741 gaacaggtgg ttcgttttac cgcgctgatc aacatcaaac cgcgtctcag taatcgggat
     1801 atggaaataa aagatcccca aatacgtgcc cacgttgaat cagtcgtcaa gaaaggtttt
     1861 gaaggagttc aatgaggaac ccagaaatct tgcgcactcg ctatctcagg gatcccctcc
     1921 ccatgcgttt ggggaatttg gcgtcgagcc tggctcgcct ggcaacatgt gttgaacact
     1981 cccgcagctg gaactcggtg gcaggtatat tggatgaaag cctgcaattc attcaatgga
     2041 ccatgccggc ggcggcccca gagccacagg ttctgctggc ggatgtacaa aaagaattga
     2101 ttcgctggaa gctggaaagg ggcttggagg gagatgcaga agcaacccga gcacttgcag
     2161 acaatggttg aagaagcgcg ggggtgggcc caggagatct tagaactgtc cgggcttttg
     2221 gacgcgcaaa aaagtcccgc gccctaaact tcaaagtctt caggtcaatt ttccatccgg
     2281 gcgttgcccg catatattgg taaagtgagg aagaagaggg gttttttcat taccttcgaa
     2341 ggtattgacg ggtcggggaa gacgacccag gccaagaagc tcgtccgtta cctgaaacgt
     2401 aaaagaatac cggtgctctt cctccgggaa ccgggcggta cgaacgtcgc cgagaagttg
     2461 cgccagatag tgctcaaagc caccggagag agaatcgcac cggcggctga gcttttcctt
     2521 tacctggccg cgcgcgcgca aatcgtccag aaccggattg ccgaggcgcg agcaagaggt
     2581 atcatggtca tctgcgaccg gttttccgat tcaaccgtcg cgtaccaggc ctacgggcgg
     2641 gggtggaatc aaaagctgat agcagaattg aatgcgatgg cttccaattc gacgctgccc
     2701 gaattgactt tgcttttgga tttacccgta cgggtcggtt tgcggcgaaa aaaaaccgcc
     2761 ttaggcggat ttccctacaa agaccggttg gaaagtgaag ggaaaagttt ccaccggcgg
     2821 gtgcggcggg gatacctcaa aaccgcccgg gccgagccgg agcggattgt ggtgatcgac
     2881 ggaaccggtt ccccggagac cgtgtgggaa gagatccgca gggtggtgga caaaaaaatt
     2941 gcatagagcg gatggagggt ttatgactcc catagagaga aaacagctcg cccgggggat
     3001 cttcttcgga ttcctctccg tcgtcattct ggcggcggct ttggcctatg gcttaggcgg
     3061 cgacctgatc ggaagatccc gcctggtgga aacgattgcc tcaagaattg agtccgatta
     3121 cgtcgattat gtgcccgccg acagtttgat cgactccggc attgccggaa tgttcaaccg
     3181 gctggacccc tactccgaat acctgccgcc cgccaattac cgctcgctcc tcgaagacac
     3241 gcagggacat ttcggcggac tggggattga aattgtggtg cggaacaatg cgatcctggt
     3301 cattgcgcct ctggaaggga cgccggcggc ccgggcggga ctgctccccg gcgaccggat
     3361 tgtgaaaatc gacgggcgaa ccgccgccgg aatgaccggc gaggaagcgg tagccaccct
     3421 gcgcgggccg caggggagcg ctgtgacgat cgaagtcaag cgtgaggggc tgtccgatcc
     3481 attggtgttg actctgaagc
//
LOCUS       DBNGZD010000022         2824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_767342, whole genome shotgun sequence.
ACCESSION   DBNGZD010000022 DBNGZD010000000
VERSION     DBNGZD010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_767342"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1109)
                     /locus_tag="AC16GM_00645"
     CDS             complement(297..1109)
                     /locus_tag="AC16GM_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922217.1"
                     /translation="MKKVVVTAVTLLLLVLSMGFPARGKTTDLQSFASAFPCPGVDTF
                     NTQFTARLVVGTDTANIFLTGPTVVKRKPTLTEPDGRLVIPTEMLQLNLVGANPLTPN
                     PNDSIRVNLSGPPTTGEVRAGAPLPGPNFPATSFFDVFAKINVDDFPTLFTYNPTNNT
                     DPAHIVANTINRLPPLNSDGCPIDSCFDGKDEYEVQSKKTYDSLGRVGPEMLRAHHCP
                     KSRRNECPPDSLPPCLVPSLTAGGLSLLAALLVGTFTYVYFRLRKVGGRMAV"
     gene            complement(1887..2726)
                     /locus_tag="AC16GM_00650"
     CDS             complement(1887..2726)
                     /locus_tag="AC16GM_00650"
                     /inference="COORDINATES: protein motif:HMM:NF039484.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922218.1"
                     /translation="MAPKKGYATVNRIFPGRYDPAKGGEQSREMGRDDPYGVTRKAEF
                     DLEFVWKAGWAVASGYLLVLCQISEKFSPLVWVALVPLLFVLRRADTKQALWYGFLWG
                     VVYFGLANLAVTLKGSVVVTHSEHFTTFASTGGSAHVISGWLLAFLCAFGFSLTTLFI
                     STLSRRALFNPILIASVWAAFELVRIKLGVAFGLLADAHHSPFLLNFANFAGLIAVSF
                     LIALANSAWVSLYEWVREKFTPAYRFASLPDFPHILIPNSQIRYKILALVPEVRGPPG
                     AAI"
BASE COUNT          705 a          799 c          711 g          609 t
ORIGIN      
        1 acaacaaccg tcttcgccga atctaccccc atattcttca aagtaacact gcaagcacat
       61 gcccagagtg tagaatactc ccaaaaaacc ataatgcaga cacaggcaac cagtacaatt
      121 tttttcatac gtacgtattt tctccctgac aagcgtaaga ttcctacagc agtttagtta
      181 cgaaaaacca gggagaggcc ccatccgcct caggccatac ggcccatatg ggcgggcagg
      241 gtctctccca cgttgcttcg cttaaaccat catctccttt ccttgcgctc catctcttac
      301 acagccattc tgccccccac cttccgcagc cgaaagtaga cgtacgtgaa cgtccccacc
      361 aacaacgctg ccaagagact gaggccccca gccgtgaggg acggaacaag acaagggggt
      421 agtgaatccg gcggacattc gtttcgccgg cttttgggac agtggtgcgc cctcagcatt
      481 tctggcccaa cccggcccag cgagtcgtac gttttttttg actgcacctc atattcatct
      541 ttcccgtcga aacaggagtc gatgggacat ccgtcggagt tgagcggcgg caaccggttg
      601 atggtgttgg ccactatgtg ggccgggtcg gtgttgttgg tcgggttata ggtgaaaagg
      661 gtggggaaat catccacgtt gatcttggca aaaacatcga agaaacttgt ggctgggaaa
      721 ttaggccccg gcagtggcgc tccggcgcgc acttctcccg ttgtgggagg cccggaaagg
      781 ttcacgcgga tggaatcgtt cggattcggt gtcagcgggt tggctcctac caaattgagc
      841 tgtaacatct cggttggaat gaccaatcgc ccatctggtt cagtgagggt tggcttacgc
      901 ttaaccaccg tgggcccggt caaaaagata ttggcggtgt ctgtacccac caccagtcgg
      961 gcggtgaact gtgtattgaa cgtatccacc ccggggcagg ggaaagcgga agcgaaactc
     1021 tggagatcag ttgttttgcc ccttgcggga aatcccatgc tgagcaccaa cagtagcagt
     1081 gtaaccgcag taacaactac ttttttcacc ttttcctcct cgcacaaaag tttaaggttc
     1141 agggtttcga ttcattcatt caaccatgac agttaagtct aggcggcccc agcaccagtg
     1201 cggaaaaacc aggagaggcc ccattcgcct taggccatac ggcccgtatg ggcggacagg
     1261 gcctctccca cgttcctttg cttaagccat catctccttt ccttgcgttt catctcttac
     1321 acagccattc tgcctgctac cctgcgccgc cggaagtaga ggtaggtaag tgtaccagca
     1381 agaatagcag caaaaccaat cattccccaa tttgtgagtg taggaatgat cgtagcagtt
     1441 agacagagtt gggctgacgc aacccctagc ggcggcactt cgcaaaattc ccacgtaacg
     1501 gcgtaattcc cacctcttcc ctgcgttata acactatcca ccgcattacc gtcggcgacc
     1561 actatggagc ctacaacttg cccctcggca ttacgacaag caacacttat cgtacctcct
     1621 gttctattcc tgatttttaa cttatctccc ggctcttgct tgtagggagg ggggggcgtt
     1681 aggacaagag ttgggggcgg tcccgtgtct ttcgtgactg taactgttac atccccagta
     1741 ggcgccggta ttgccactgg agtaaaggca taggtcgcta aaaacattac taaaagacca
     1801 atcaccacgt tgcttgctct agatactcgc attgcaactc ctccttgaaa agatgtgtgg
     1861 ttactactcc actcaccgta ccaaccttat atagccgcac ccggcggccc ccgcacctcc
     1921 ggcaccagcg ctaaaatctt atatcggatt tgggagttag gaattaggat atggggaaaa
     1981 tctggcaaag aagcaaagcg ataggcgggt gtaaactttt ctctaaccca ctcgtagagc
     2041 gaaacccagg cgctgttggc cagggcaatg aggaacgaaa cggctataag cccggcaaaa
     2101 ttggcaaaat tgagcaggaa tggggagtgg tgtgcatcag ccagaagacc gaaggcgact
     2161 cctaatttta tccgcaccaa ttcaaacgcc gcccacaccg aggcaatgag aatgggattg
     2221 aagagcgcgc gccgtgaaag agtggagata aaaagggtcg tcagactgaa cccgaaggcg
     2281 cacaagaaag ccaaaagcca cccggaaata acgtgggcgg agccgccagt agaggcaaaa
     2341 gtggtaaagt gttcggaatg agtgacaacc accgaaccct tgagcgtgac cgccagattc
     2401 gccagcccga agtaaaccac cccccagagg aacccgtacc agagcgcctg cttggtatcc
     2461 gcccggcgca gaacaaaaag gagtggcacc aaagccaccc agactaaagg actaaatttc
     2521 tcggaaatct ggcaaaggac taacaggtac ccgctggcca ctgcccagcc ggctttccag
     2581 acaaactcaa gatcaaattc tgcttttctc gttaccccat acgggtcatc ccgacccatc
     2641 tcccggctct gttccccgcc cttggcgggg tcgtaccgac cggggaaaat ccgatttacc
     2701 gtagcatagc cctttttagg cgccaaacgc ggacaagccg ctatgctaca cgtccacttt
     2761 ccaatctgtg ccgactaaag cagtgctctt cgtcttcatt cgggtcgtag accctgagcg
     2821 aagc
//
LOCUS       DBNGZD010000023         3292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1600797, whole genome shotgun sequence.
ACCESSION   DBNGZD010000023 DBNGZD010000000
VERSION     DBNGZD010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1600797"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1179
                     /locus_tag="AC16GM_00655"
     CDS             94..1179
                     /locus_tag="AC16GM_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010597145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR2922219.1"
                     /translation="MTASPKRKIIDTFPELIFLASSMRFSHSILDLIGHTPLVKLSKM
                     APPPGPTILAKLEYTNPGGSVKDRMVWHIVREAEKAGLLKLGDTIVDNTSGNTGIGLA
                     LVAAVRGYHAILAVPDRVSQEKIDLLTALGAEVIVTPSDVPIEGPRSYYQTAQRLARE
                     RGAFHLNQYHNPKNPEAHYLTTGPEIWEDTAGKIDVLVAGIGTGGTITGIARFLKEQK
                     PQIKVVGVDPVGSIHAEFIRTGRPAAPEPYLLEGIGSEMVTQALDPEVVDTVLQVTDR
                     DAFLMVRKLAQKEGLLVGGSSGAAFWAALEVARGMDSQSVIVTIFPDSGTRYLSKYFN
                     DEWMRARGFLAPLRGHRSRVPSAVSSP"
     gene            1271..2047
                     /locus_tag="AC16GM_00660"
     CDS             1271..2047
                     /locus_tag="AC16GM_00660"
                     /inference="COORDINATES: protein motif:HMM:NF046853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR2922220.1"
                     /translation="MQDKRKQFFLAAFFALMFCLAGAKGTLAQTPPESTHAPAAAFKS
                     GTLSLSEKSWDFGYIPKGSRVTHTFVIKNNGTDSLKNLKASPSCGCTAAPLDKTILAP
                     GDSTYLEVSFNAKNYSGPVTKTVYLQSNDPQNPYFDLLFTANVGTAIPNLSVTPPEVT
                     FDTLREIPNSPTKLEIKNINTDNKIGLEVIYAPKPFVDYKLTKKALKPGQAGEVSVRL
                     KSDKMKPGEFFTSLTILCNDPNKSRFTVPIHGVYAANATK"
     gene            complement(2260..2703)
                     /locus_tag="AC16GM_00665"
     CDS             complement(2260..2703)
                     /locus_tag="AC16GM_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922221.1"
                     /translation="MKWKNNKPIKILLFIAIPIVLLPVGFFLLYLLFGGDWGIDLPGG
                     YFLSSIYGGAVEITDSPKNIVVYPNIETYKVYEQKHLVVGRVSNEGLQPREVENSVPG
                     YFILNTENGLLLQGLEEKVWLDSLRRYGITQKPRLHKPTGFDRNY"
     gene            complement(2830..>3292)
                     /locus_tag="AC16GM_00670"
     CDS             complement(2830..>3292)
                     /locus_tag="AC16GM_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase"
                     /protein_id="HIR2922222.1"
                     /translation="VSRVRNDHLGMMEAAGRSGVNFVGGTRGGFIFPAFQPGVDAMFA
                     AAKFLELLARSQKKISTLVAELPAYPMLTEEVPCPWEKKGQVMRRLLEETAHVERELV
                     DGVRIRNADGWILVWPDRRSGTFTLTAEGRDPEQVKREVAEYRRLIELWQK"
BASE COUNT          757 a          985 c          847 g          703 t
ORIGIN      
        1 aacgcaaccc ggaatgcaga ctctcctccg ccgaccacct gatttccgat cttgagctgt
       61 gcgggctggc tgccgaagaa agaaaaggag gagatgactg ccagcccaaa gcggaaaatc
      121 attgacacgt tccccgaatt gatttttctt gcctcatcca tgcgcttttc ccattcgatt
      181 ctcgacctca tcggccacac acccttggtc aaactttcca aaatggcacc gccccccggc
      241 cccaccatct tggccaagct ggagtacacc aaccccggcg gttcggtcaa agaccggatg
      301 gtctggcaca tcgtgcggga ggcggagaaa gctggattgc taaaacttgg ggacaccata
      361 gtggataaca cctcggggaa taccggtatt gggctggcgc tggtggccgc cgtacggggc
      421 taccacgcga ttctggcagt ccccgaccgg gtgagccagg aaaaaatcga cctcctcacc
      481 gctctgggtg ccgaggtgat cgtcaccccc tcggatgtcc cgatcgaggg tccccggtca
      541 tactaccaga ccgcccagcg ccttgcccgg gaacggggtg cttttcacct gaaccagtat
      601 cacaacccga agaatccgga agcccactac ctgaccacgg gtcctgaaat ctgggaggat
      661 acggcgggga aaattgacgt gctggtggcc ggcatcggca ccggcggcac aatcaccgga
      721 attgcccgtt ttctcaagga gcagaagccg cagatcaaag tggtcggagt cgacccggtc
      781 ggttccattc atgccgagtt catccgcacc ggcagaccgg cggcgccgga accgtatctc
      841 ctggagggca ttggctcaga aatggtgacg caggccttgg atccggaggt cgtcgatacc
      901 gtcctccagg tgaccgatcg cgatgctttc cttatggtcc ggaaattggc tcaaaaggag
      961 ggtctgctcg tcggcggctc ctccggcgcc gccttttggg ccgcacttga agtggcccgg
     1021 ggaatggact cccaaagcgt catcgtaacc atcttccccg attcgggcac gcgctacttg
     1081 agcaagtact tcaacgacga gtggatgcgc gcccgcggct ttttggcgcc tttgcggggt
     1141 cacagatccc gggtcccctc cgccgtttct tccccctaaa aaaaagccaa cgtctcgtcc
     1201 ggtggacacg tttccctatc gatacgcctg acggagaaga gaggaaccca aagaacagga
     1261 gcggtgaggt atgcaggaca aacggaagca attttttctc gcagcgtttt tcgcactgat
     1321 gttttgtctg gcgggcgcaa aaggtacact ggctcagacc cctccggaga gcacccatgc
     1381 tccggcagcc gccttcaaat cgggcacact ctccctgtcc gaaaagtcgt gggatttcgg
     1441 ctacatcccc aaaggttccc gcgtcaccca taccttcgtc attaagaata acgggaccga
     1501 ctcgctgaag aacctgaagg cttccccctc ctgcggctgc accgcggcgc ctttggataa
     1561 gacgatcttg gctccgggcg attcgaccta cctggaagtc tccttcaacg ccaaaaacta
     1621 ctccggcccg gtgaccaaga ccgtctacct gcagtccaat gatccgcaga acccgtactt
     1681 cgatttgctc ttcactgcca acgtgggcac cgccatcccc aacctctcgg tgactccgcc
     1741 ggaagtgacg ttcgacacgc tgcgcgaaat tccgaattcc cccacgaagc tcgagattaa
     1801 aaacatcaac accgacaaca aaatcggcct cgaggtgatt tacgcgccca agccgttcgt
     1861 cgattacaaa ctgaccaaaa aggcgctcaa acccggccag gcgggggagg tttcggtgcg
     1921 gctgaagtcg gacaaaatga aaccgggtga attcttcacc tcgctcacga ttctctgcaa
     1981 cgaccccaac aagtcgcgct tcaccgtgcc gatccacggt gtttacgccg ccaacgccac
     2041 caagtgatca ttgcgttccg aagcgagacg acctgcccct tgacagaacc ggtcaggggg
     2101 catttgttat actggagaat tcagagggtg gacctccgtt catggggtag tttccaagcc
     2161 ccctctaccc caccggcgca acaccctcat ttattttttg ggaagtgaga agccaaagaa
     2221 aaggggctgt tctcaaacaa ataacacaac acggaatcat caataatttc gatcaaatcc
     2281 ggtcggtttg tgtaagcgcg gcttttgcgt tattccgtat cttctaaggg aatccagcca
     2341 aactttctct tcaagccctt gtaatagtag gccattttca gtattaagta tgaaatagcc
     2401 gggcacactg ttctcaactt cacgcggttg caggccttcg tttgacacac gccccaccac
     2461 aaggtgtttt tgctcataaa ctttatatgt ttcaatattg gggtaaacga caatattttt
     2521 cggggaatca gtgatttcta ccgctcctcc atatatactg ctaaggaaat agccaccggg
     2581 gagatcgata ccccagtccc cgccgaatag taaatacaaa agaaagaatc cgacgggtaa
     2641 caatactata ggtatggcta tgaagagtaa aatttttatc ggcttattgt ttttccattt
     2701 cattataaaa tccgtcgatg ccggcaacac ttgcacgcca cgatgcgtta atcgggtgag
     2761 aaggacctaa actttaatcc ccatacaagt tgagaaaaaa tgagttctgc cctaaagcga
     2821 tagttgcctt tatttttgcc acaactctat taaccgccgg tattctgcaa cctcccgctt
     2881 gacctgttcg gggtcgcgtc cttctgccgt gagggtgaag gtgccgcttc gccggtcggg
     2941 ccagaccaga atccagccat ctgcgttgcg gatgcgtacc ccgtccacca gttcacgctc
     3001 gacgtgagcg gtttcttcga gcagccgccg catcacctgg cccttttttt cccaggggca
     3061 gggcacctct tcggtcagca tcgggtaggc ggggagctcg gctaccagcg tcgaaatttt
     3121 cttttgcgaa cgcgccagaa gctccaaaaa cttggccgcg gcgaacattg catcgactcc
     3181 cggctggaag gccgggaaga tgaatccccc gcgggtcccc ccgacaaaat tgaccccgga
     3241 gcgaccggcg gcctccatca tgcctaagtg gtcgttgcgg acgcgggaaa ca
//
LOCUS       DBNGZD010000024         8237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1392872, whole genome shotgun sequence.
ACCESSION   DBNGZD010000024 DBNGZD010000000
VERSION     DBNGZD010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1392872"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="AC16GM_00675"
     CDS             complement(<1..538)
                     /locus_tag="AC16GM_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR2922223.1"
                     /translation="MRRLALVLLGAGFLLLGGITAGAQEKKIGFVDSGKIRDGFKEFQ
                     KGQDELDSVIAGYQKKEEELRKEIEDLQDELERQRLVLSETKRKEKEQELTQKQKVYQ
                     DWYTSTFVGPQSLLEQERQKRSRPILDKILTACKKVGQANGYSFVFDSAAGSLLYGDA
                     AFDLTDKVLEELNKSAAPT"
     gene            complement(623..1639)
                     /gene="gmd"
                     /locus_tag="AC16GM_00680"
     CDS             complement(623..1639)
                     /gene="gmd"
                     /locus_tag="AC16GM_00680"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR2922224.1"
                     /translation="MKRALITGITGQDGSYLAEFLLSKNYEVWGMVRRASTENFDRIN
                     HIRDQIILKQADLLDQLSIITILKECQPNEIYNLAAQSFVPTSWDQPVLTGEFNALGV
                     TKVLEAIRLVDKKIKFYQASSSEMFGKVREVPQTEKTPFYPRSPYGVAKVYGHFITVN
                     YRESYGIFATSGILFNHESPRRGLEFVTRKVSDGVARITLGLSKELALGNLDAKRDWG
                     FAGDYVKAMWLMLQQNEPRDYVIATGKNHSVRELVQVAFQHVGLDWEKYVVVDPKFVR
                     PAEVDVLLGDATLAKTKLGWQPEESFEGLVKMMVDHDVKRVLSGMQAQPPGTKTRSPG
                     VRVQ"
     gene            1757..2944
                     /locus_tag="AC16GM_00685"
     CDS             1757..2944
                     /locus_tag="AC16GM_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2922225.1"
                     /translation="MISIKKFAALRIFLTWFGAGGIFFSSLVETQDRLGGEVVASQAN
                     RKLPGGRGRGKGWIAPLFACFLLLPQLAGAYPFVDSLIVASKTFSFGSETVLEIFLYN
                     DSAVFGMSIPLVIKSQTGGAFGRVDSIKMVLPVAYPPAFDLKGVNGTDPDSLFILWVD
                     PYGGYIPGSSQRREAFQLFVTNNANVGVYSLDSNRLSSAKTLKVCHIISLPCLVTFVR
                     GVETILNDPPLLRCSLGRQVYFGASVSLLPDEASDPNGDPLTFSLVSFVKAGGGSPPQ
                     YAPVVDSTTGRFFWRSAFDVTELGAWEVTIGVMDPFPRPPVTCSFTIEVADLRGDVNN
                     DGLWSDLVDVLELINAVAFQSPITSENPNAADVNCDGMTDMVDIIDLINFVVFGTPMP
                     CRG"
     gene            3257..3586
                     /locus_tag="AC16GM_00690"
     CDS             3257..3586
                     /locus_tag="AC16GM_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922226.1"
                     /translation="MGAKKRGWIKWVAGTLAGVCLLAAPLWGGPPVTPYDTDDHPWEG
                     NPYGPPTSGGGHGIVIIVKGSGPWVGVPYFWNIAVIKTQGTSTRTASQSASGGKGSAR
                     VEKKAGR"
     gene            3593..6502
                     /locus_tag="AC16GM_00695"
     CDS             3593..6502
                     /locus_tag="AC16GM_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR2922227.1"
                     /translation="MQVRPAPETIRSERLAHIAQLVGLRRFTEAIAEIRDFDQSASGG
                     AGKVSPAEWAEACFYAAQAFHSVGNYTEALSRAEAAYEYYKPTSENEKIARLQFLLGL
                     IYLGLGKLPEAEAELRDAYSTSRRIGDMAGMIDALNRQANIAFLRFDHAQAISELTTA
                     IELARGTDDRARSAWLTGNLGRIHLFLGERSKAIECLRVSLEHNRATGDEPNLSRTQL
                     SLGFAYLLSRNLAVATEFLLSARELIEKHNLSRELGIFHEYYGQLLMEKGDFAAARRE
                     YEAGIAIAEKIGPESNLASQLYRLYAECAFEQGDYDLARSYCERGMSVATRLNERIEL
                     GVLHRLSARLALVAQSPAEAERHLRESIAIFEQIGVKHELAQTLIVAAESGLFAPQVV
                     ADQFLRAEKLFAELNDREGLFAACRREIELFGKQEDYDRALDAIARAETDARRLLELR
                     EATAFQTLRRKIEERVAELATSSANEYHFFSPAFAGGSPTEMNQVAEAMLLLMQPVQA
                     TCGILALLEKTPRSQSTAAEAARFSVVWQKDIPAALSPHLEAVFDRVQESRKPAWYSD
                     CRTESAFGGLRSAIVIPFTWARERLAVILLGIHDSAAGPVRQFAKTDFFFAAAFAEFV
                     ALRLVDEERRHLEDENRRLRRQLEERCTFTSVVTQDPALLGMLDRLELVKDSELPILL
                     EGETGTGKDLVAKAVHYASKWKTGSFVPVSCSALPETMLESELFGHKKGSFTGAATDK
                     VGLIEEADGGTLYLDEVAEIPLSIQVKLLRVLEEKELVRLGETRPRKVNCRIISSTNR
                     DVRQAVTEGRFRKDLYYRLNAVNVVFPPLRQRKEDLPLLIDHYLTKLSRNESLPTGNG
                     IPVRLAAEVLRAFYHYDWPGNVRELVNELSRILVLAKGVPVIGAEYLSDKFTSAAASP
                     LLPGSLETRLSQYERDLIEKALSASDRVKAQAARILGIPVTTLRYKMGKHGLNWK"
     gene            complement(7008..7379)
                     /locus_tag="AC16GM_00700"
     CDS             complement(7008..7379)
                     /locus_tag="AC16GM_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922228.1"
                     /translation="MRTIGEFYREEVLPHKPLSKRQLPTQRDKSEIVKDLFGWKLYSG
                     KEFIDCRSEEEARFLKVFLDAGMQEVKVPKDDRILKEILPKLERLKRNIDEIIEEHAD
                     GLLSPRLKEELRHRVWQELVS"
     gene            complement(7376..>8237)
                     /locus_tag="AC16GM_00705"
     CDS             complement(7376..>8237)
                     /locus_tag="AC16GM_00705"
                     /inference="COORDINATES: protein motif:HMM:NF046985.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922229.1"
                     /translation="TPKYVKPIIVSLDETESIVIRVAKLPKSVFWANEEKSKLPKSIL
                     QYIRQGENRTFSKGKRSYIPAKTKTCSSRPLWYSLGERDPAPILHPLYMGDRFLILFN
                     REGVLTNQNLHEVYPLYKEDDEAEVLCAIMNSIVFQLCHDLHGRNLTGAINVIGVEMG
                     LLSQVPIIDTAQIPSSTRLDLLRAFRKLSVRPISHILDELGVFSGGNVSFNTAKPDRR
                     ELDKIIMRDILGLKEEEQLEVYKAVVDLVRSRIEKAQSVEKGKRYIEGVDVDALAGGV
                     VKQVKGKKEK"
BASE COUNT         1761 a         2347 c         2230 g         1899 t
ORIGIN      
        1 gtgtcggagc ggcggacttg ttcaactctt ctaacacctt atcggtcagg tcaaaggccg
       61 catcaccgta caggaggcta cccgcggctg aatcgaacac gaaggagtag ccgttggcct
      121 ggcccacctt tttgcaggcg gtcagaatct tgtccaatat cggacgggag cgcttctgcc
      181 tttcctgttc caaaagactc tgcggaccga caaaggtgga ggtgtaccag tcctggtaca
      241 ctttctgctt ctgagtcagc tcctgctcct tttccttgcg tttggtttcg ctcaaaacga
      301 gccgttgccg ctccagttcg tcctggaggt cctcgatttc cttccggagt tcctcttctt
      361 tcttctggta gccggcaatg accgagtcga gttcgtcctg gcctttctga aattccttga
      421 acccgtcgcg gatcttcccc gaatcgacaa agccgatttt cttttcctgc gctccggcgg
      481 ttattccacc aagcagcagg aacccagctc ccagaagcac cagcgccaat ctacgcacca
      541 tacctccctt gctgagcgtc ccacatatta ctatttgtct tggtctcgtt gtctcgcaga
      601 ggaccggtca ggattctcct ggtcactgga ccctgactcc cggcgaccga gttttggtac
      661 ccggcggctg cgcctgcatt cccgaaagaa ctcttttgac gtcgtgatcc accatcattt
      721 tcaccaggcc ctcaaaactc tcctccggct gccagcccag cttggttttg gccaaagttg
      781 catctcccag taatacgtcc acttcggccg ggcggacgaa cttcggatcg accaccacgt
      841 acttctccca gtccaggccc acgtgctgaa aggcgacctg aaccaactcc cgcactgaat
      901 ggttcttgcc ggtggcgatc acatagtccc gaggttcatt ctgctgaagc atcagccaca
      961 ttgctttcac gtaatcgccg gcaaaacccc agtcgcgctt ggcgtctaag ttgccgagcg
     1021 ccagttcctt agacagcccc aaagtaatcc gagccacccc gtcggaaact ttgcgggtga
     1081 cgaactccag cccccgccgg ggcgattcgt ggttgaagag aatcccgctg gtggcgaaaa
     1141 tgccgtaact ctcccggtag ttgacggtga tgaaatgccc gtagactttg gccacgccgt
     1201 aaggcgagcg ggggtaaaag ggggttttct cggtctgcgg cacttcccgc accttgccga
     1261 acatctccga agatgaggcc tggtagaact tgattttttt gtccaccagc cggatcgctt
     1321 ccagcacctt cgtcaccccc agggcattga attcccctgt caataccggc tgatcccagg
     1381 aagtggggac gaacgactgc gcggccaaat tgtagatctc gttcggctga cattccttca
     1441 aaatcgtgat gatcgagagt tggtcgagca ggtcggcctg cttcaggatg atctggtcgc
     1501 ggatgtggtt aatccggtca aaattttcgg ttgacgcccg gcgcaccatc ccccagacct
     1561 cgtagttttt ggaaagcaga aattcggcca agtacgagcc atcctggccg gtaatgccgg
     1621 tgatgagcgc ccgcttcatt gagtgattct ctgcagcata gggacgctaa ttaactacag
     1681 ggaagggggt acgtcaattg ccggaattgg gattggggat ggggttggtt acctgtcaaa
     1741 gggacaagca aagtctttga tctccattaa aaagtttgcc gcactaagaa tttttttgac
     1801 ctggttcggc gcaggtggta tattcttctc gagcctcgtt gagactcaag accgactcgg
     1861 aggagaagtg gtcgcaagcc aagccaaccg gaagctccca ggaggcagag ggagggggaa
     1921 aggttggatc gcacccttat ttgcctgttt tttgctcctc ccgcagcttg ctggggccta
     1981 tccatttgta gattccttaa tagtcgcgtc caagaccttc agtttcggaa gtgaaaccgt
     2041 tctcgagata tttttataca atgactcagc tgtctttggg atgtctattc ctctggtaat
     2101 caagtcgcaa actggcggag cgttcggaag agttgattcc atcaagatgg ttctgcccgt
     2161 agcttatccg cccgccttcg atttgaaagg agtaaatgga acggatccag actctctatt
     2221 catactctgg gttgatcctt atgggggtta catcccaggt agttcgcagc gccgtgaggc
     2281 atttcagctg tttgtgacca ataacgccaa tgtaggggtg tactcgctgg actctaatcg
     2341 gctctcctcc gccaaaacct tgaaggtctg tcacataata agcctgcctt gtttggtgac
     2401 attcgtccgc ggagtggaaa cgattctaaa cgaccctccg ctcctgcgct gctctctcgg
     2461 tcggcaagtc tactttggag cgtcggtctc tcttttgcca gacgaagcga gcgacccaaa
     2521 tggtgatccc ctcacctttt cattggtcag tttcgtgaag gcaggtggag gcagtccgcc
     2581 acagtacgct ccggtcgtag acagcaccac cggtcgcttt ttctggcgta gtgccttcga
     2641 tgtgacagag cttggcgcgt gggaggtaac tatcggcgtc atggaccctt tcccgcgtcc
     2701 tccggtcacc tgtagcttca ccatagaagt agcggacctt cgcggagatg taaacaatga
     2761 cgggctgtgg agcgatctgg tggatgtgct tgaactgatt aacgctgtcg cattccagtc
     2821 tcccataacc tcggaaaatc ctaatgcggc agatgtgaac tgtgacggaa tgaccgatat
     2881 ggtggacatt atcgacctta tcaatttcgt ggtgttcgga actccgatgc cttgccgggg
     2941 ataggtcttg cacttacccg cacacaggag ttatcttacc agcgtatact tacctgcaag
     3001 aaatctcttg actttttgga ggatggcggg tattttttct tctgagggtg ggggtatgaa
     3061 gctggaagcg ggcaggttcg ccgcctgctc gacggatata tcggtcgtgc gggaggcgcc
     3121 cgcactgcaa aagaagttcg ccacaaggca ttgaaaatga agaagatgcc acgtccgcca
     3181 cacgtcaggc aagtcaccgg cggttcgtat cgagggccgc atggttggga gcggtccaag
     3241 taaaggagtc cggcttatgg gcgcgaaaaa acgagggtgg atcaagtggg tggcaggtac
     3301 tctggcggga gtctgccttt tggcggcgcc gctctggggc gggccaccgg ttactccata
     3361 tgacactgat gaccatcctt gggaagggaa tccgtacggg cctccgacga gcggcggcgg
     3421 ccacggaatc gtgattatcg tcaaaggtag tggcccgtgg gtcggtgtcc cctatttctg
     3481 gaacatcgcc gtgatcaaaa cccagggcac ttccactcga accgcgtccc aatccgcctc
     3541 tggcggaaaa ggaagcgcga gagtcgagaa aaaagcaggt cgatagccga ctatgcaagt
     3601 acgccccgcg ccggagacga ttcgatccga acgtctcgcg cacattgccc aattggtcgg
     3661 cctgcggcgg ttcaccgaag cgatcgccga aatccgagac tttgaccagt ccgcctccgg
     3721 cggagcaggc aaggtgtctc ccgcggagtg ggcggaagcc tgcttttacg ccgcccaggc
     3781 ctttcactcc gtggggaact acaccgaggc cttaagccgg gcagaggccg cctacgagta
     3841 ctacaaaccg accagcgaaa acgagaagat tgcccgactg cagtttcttt tgggcttgat
     3901 ttacctcgga ttggggaaac tccccgaggc cgaggcggag ttgagggacg cctactcgac
     3961 gtcccgccgt ataggcgata tggcgggaat gatcgatgct ttgaaccgcc aggccaacat
     4021 tgcttttctc cgcttcgatc acgcgcaggc aatttcagag cttaccacgg ccatcgaact
     4081 ggcgcgcggc accgatgatc gcgcccggag cgcctggctc accggcaatc tgggccgcat
     4141 tcacctcttt ttaggcgaaa ggtcgaaagc catcgaatgc ctgcgggtga gtctggagca
     4201 caaccgggcg accggcgacg agcccaacct ttcccggacg cagctttcgc tcggttttgc
     4261 ctaccttctg tcgcgcaacc tggcggtggc cactgaattt ctcctttcgg cccgggagtt
     4321 gatcgaaaag cacaaccttt cgcgcgaact gggcattttc cacgagtact acgggcagtt
     4381 gttgatggag aaaggcgact tcgccgccgc ccggcgcgag tacgaagccg gaatagccat
     4441 tgccgaaaag atcggcccgg aatcgaattt ggcctcccag ctctaccgcc tgtacgcaga
     4501 gtgcgcattc gaacaggggg attatgactt agcccggtcg tactgtgagc gggggatgtc
     4561 agtcgccacc cgattgaacg agcggataga actgggcgtc ctgcaccgtc tttccgcccg
     4621 gctggcgctg gtggcccaat ctccggctga ggccgagcgc cacctgcgcg aaagcatcgc
     4681 cattttcgag cagatcgggg tcaagcacga gctggcccaa acgcttatcg tcgctgcgga
     4741 gagcgggctg ttcgctccgc aggtcgtcgc cgatcaattc ctgcgagccg aaaaactttt
     4801 tgcagagctg aacgatcggg aaggactctt cgccgcctgc cgccgcgaaa tcgaactctt
     4861 tgggaaacag gaagactatg atcgcgccct ggatgccatt gcccgggcgg aaaccgatgc
     4921 ccggcggctt ttggaactcc gggaagcgac ggcgttccaa acgctccgcc gcaaaatcga
     4981 agagcgggta gccgagctcg ccacctcctc cgcgaacgaa taccatttct tttcgccggc
     5041 gtttgcgggc ggcagtccga ccgaaatgaa tcaggtggcc gaggcgatgc tcctcttgat
     5101 gcagccggtg caggcgacct gcggcatcct ggcgcttttg gaaaagacgc cgcgttccca
     5161 atcgaccgcg gctgaagccg cacgattcag tgtcgtctgg cagaaagata ttcccgccgc
     5221 gctgtcgcca cacctggagg ccgttttcga ccgcgtgcag gaatcccgga agccggcctg
     5281 gtactccgac tgccggactg aatcggcctt tggcggactg cgctcggcca tcgtcattcc
     5341 ttttacctgg gcgcgggagc ggctggcggt cattcttttg ggcatccacg attcagcggc
     5401 agggccggtg cgccagttcg ccaagaccga tttcttcttt gcggcggcct ttgccgaatt
     5461 cgtggccttg cggttggtcg acgaagagcg ccggcactta gaagatgaaa accgccggct
     5521 gcggcggcag ctggaagagc gctgcacctt taccagcgtg gtgacgcagg atcccgcgct
     5581 tttggggatg ctcgaccggc tcgagttggt aaaagattcc gaactcccta tccttttgga
     5641 aggggagacc ggaaccggca aagatctcgt ggccaaagcg gtgcattacg cttccaagtg
     5701 gaaaaccggc agcttcgtgc cggtgagctg ttccgcgctg ccggaaacga tgctggaatc
     5761 ggaactcttc gggcacaaga aagggtcttt caccggcgcg gccaccgaca aagtcgggct
     5821 gattgaagaa gccgacggcg gcacgctcta cttggatgaa gtggccgaaa tcccgctctc
     5881 tattcaggtg aaactcctcc gggtgctgga ggaaaaagaa ttagtgcgct taggcgaaac
     5941 ccggccgcgc aaggtcaact gccggattat ttcctccacc aatcgagatg tccgccaggc
     6001 ggtgaccgag ggccggttcc gcaaagacct ctactaccgg ctcaatgcgg tcaacgtcgt
     6061 ctttccgccc ctgcgccagc gcaaagaaga tctgcccctg ctcatcgacc actacctgac
     6121 caaactctct cggaacgagt cgctgccgac cggcaatggc attcctgtgc ggctcgccgc
     6181 cgaagtgctc cgcgctttct accactacga ctggcccggg aacgtgcggg agctggtcaa
     6241 cgaactctcg cgcattcttg ttttggccaa aggggtgccg gtcatcggag cggagtacct
     6301 ctccgacaaa tttacttccg cagccgcctc cccgcttttg ccggggtctc tggaaacgcg
     6361 cctctcccag tacgagcgcg acctgatcga aaaggcgctc tccgcctccg accgggtgaa
     6421 agcccaggcc gcccgcattc tgggcatccc cgtcaccacc ctgcgctaca agatgggcaa
     6481 gcacgggctg aactggaagt agttcttcgt tcaaaagcat aattccacca cagaggcacg
     6541 gagggcacgg agaaaatcgt gaactctccc tccataaaat ccgtttaatc tcgtagagtc
     6601 gtaccgaccg ctccaaaatc cgttgtccca tacgggtcgt accgacagaa tttggggtta
     6661 cgacaggcgt aaaacggtct cttttctcct ccgtaggcgg ggcgtctcgc cccgcttaat
     6721 tcggggtcat cgcggaggta aaacggacaa agtgcgaaat agtagagggt cgctagctcg
     6781 accctcagag actggtgccg agaaccggcc tctgcggact ggcctccttc aagccgcgcg
     6841 cgttaaacaa agagcttcaa gcgccagtgc acgccggtcg gtccgataaa gtaggatcgg
     6901 gcatcacttc tcgtcaggga gaaaaaggcc ccggggccgc cctggaaaaa acagggcaaa
     6961 agaaagcaaa agaagatgaa agtgaaaggg attagggcaa aaatgaatta acttaccagt
     7021 tcctgccaga cccggtggcg gagttcttct ttcaggcgtg ggctcaaaag gccgtcggcg
     7081 tgttcttcga taatttcgtc tatgtttctt ttcagccgct ccagtttggg caatatttct
     7141 tttaaaattc tgtcatcttt gggcactttc acttcttgca tcccggcatc caaaaagacc
     7201 ttcaaaaacc gggcctcctc ttccgagcgg caatcgatga actccttccc ggagtagagc
     7261 ttccagccaa aaaggtcttt gacgatttcg cttttatccc tttgcgtcgg cagttgcctc
     7321 ttggacagcg gcttgtgcgg gagcacttct tcgcggtaga attcccctat cgttctcatt
     7381 tttccttttt tcccttaacc tgttttacca cgccgcccgc cagcgcgtcc acatccacac
     7441 cttcaatata tcttttaccc ttctccacgc tctgggcctt ttcgattctc gaccggacca
     7501 aatctaccac tgccttataa acttccaact gctcttcttc cttcagccct aatatgtccc
     7561 gcattattat tttatccaac tcgcgccggt caggcttggc ggtattaaaa gagacattac
     7621 ctccagaaaa tactcctaat tcgtctaaaa tatgtgaaat cggacgaacg ctaagcttgc
     7681 ggaacgctcg cagaagatcc agcctagtgc tacttggtat ttgggctgta tcaattatag
     7741 gaacctgact caacaacccc atctcaactc caattacatt aatagctcct gttagattac
     7801 ggccatgcaa gtcatggcat aattgaaaaa caatagaatt cattattgcg cataaaactt
     7861 ctgcttcatc atcttccttg tagagtggat aaacttcatg aagattctga ttagttagaa
     7921 cgccctccct attgaacagt attaagaatc tatctcccat gtacaaagga tgaagtatag
     7981 gtgcagggtc tctttcgccc agcgaatacc atagtggtct tgaagagcaa gtcttcgttt
     8041 tggccggaat gtagctccgt ttcccctttg aaaatgttct gttttcaccc tgtcgtatat
     8101 actgcaagat acttttagga agctttgact tttcctcatt tgcccaaaaa acacttttgg
     8161 ggagtttagc gacccgaatg acaatggatt cagtttcatc cagtgaaaca ataataggct
     8221 taacatattt gggggtc
//
LOCUS       DBNGZD010000025         2885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1254069, whole genome shotgun sequence.
ACCESSION   DBNGZD010000025 DBNGZD010000000
VERSION     DBNGZD010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1254069"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..2655
                     /locus_tag="AC16GM_00710"
     CDS             61..2655
                     /locus_tag="AC16GM_00710"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR2922230.1"
                     /translation="MQFTSIFPSVSIGRTGFVDGQFKGGYWYPTVAVYDHKMGWVNNQ
                     FLGAGEAYGEFGTFDVSLTFPAHFIVAATGLLQNRNEVLPESLCQKLRIENFKDKPWG
                     SPASSVIPKEEGKNKTWRFRAENVHDFAFIADPTFRIGEAEWEGIKIYAYCQEKHASG
                     WQDAAALGARAIQYFSETFGRYRYPQMIIGDSYDGMEFPMLVMCGGRSPSYRLVVYHE
                     IGHNWYMGQVGSNQVDRAFLDEGFTTFMEINCIETFHGRKGNINFFDKWYEKKYYPQD
                     EDRAARGYRPYYQWSKSGYAQRMIISADKAPENNVYRVSSYYKTVALLFNLQYVLGDS
                     LFWQGMRTYFERWHFKHPYEDDFQTVMEEVSGRELDWFFDEWIHTTKTIDYAVARPKG
                     KWVETDSGKVYRTQLRLSRKGESIMPLDLRVTLKNGQARDFLIPLDESAKPTFAQILP
                     IWPQVREIRKKYAATVDLPAKPKRIEIDPSGRLADVNPLNNRYPIPKVELQLDNLKYN
                     PQPVDAYLLTARPQIWFNSRDAVQLGLHVTGGYLGFTRQLSAEGVSATRRIKGHFAVK
                     ATHPFEALGRLAFWSVSARDYDGRRQFAFELSKQIRNRWGYPPVVEHRLISGFERAYD
                     SRYLFDQSSWATTTVPYAAYLFSGHWPKRWGALRVQFQNESGAKGRFISDPAFNRTSG
                     LVRIAWNVVPNWTLLFRLFGSHYIGARVPQKEFRLNGAEPEQVFDLPLLRSKGTIPNR
                     WQKHFRVVGGGNLRGVENPPLGAENIIARTIEVLPPNLVSLSLLRKIPLAGNFLAGMR
                     NYAFFDDGILMFDGAKDRYFYDLGVGFKFPNIWKNQKFQIDFPIFVGSDRRLEPVKFR
                     WVVGWEVE"
     gene            2690..>2885
                     /locus_tag="AC16GM_00715"
     CDS             2690..>2885
                     /locus_tag="AC16GM_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922231.1"
                     /translation="MHQTQTRPRRAGGEEKVRNLILEKWFHLTTRGTNVRTEVLAGVT
                     TFLTMSYIIFVNPAFLSFSAD"
BASE COUNT          657 a          792 c          792 g          644 t
ORIGIN      
        1 actatagcac gtgtagaatt ggctgggccg ctgccgcccg gcgctgagtg tgcgtttgaa
       61 atgcagttca ccagtatttt cccctcagtt tcaattggtc gcaccggatt tgtggatggt
      121 cagttcaagg gcggctactg gtatcccacc gttgcggtgt acgaccacaa aatgggctgg
      181 gttaataatc agtttttggg tgccggcgaa gcctacggcg agtttgggac gttcgacgtg
      241 tcgctgacct ttcccgccca cttcatcgtc gcggccaccg gcctcttgca aaatcgaaac
      301 gaagtgctgc cggaatcgct ctgccaaaaa ctcagaatag agaatttcaa agacaagccc
      361 tggggctcac cggcgtcaag cgtcatcccg aaagaagaag ggaaaaacaa aacctggcgt
      421 ttccgcgccg agaatgtcca cgattttgcg tttattgccg atcccacttt tcgcattggc
      481 gaggccgaat gggagggaat taaaatttac gcctattgtc aggagaagca cgcctccggc
      541 tggcaggatg cggcggcatt gggtgcgcgg gcgattcagt atttctccga aaccttcggc
      601 cgctaccgct acccgcaaat gatcatcggc gattcgtacg atgggatgga attcccgatg
      661 ctcgttatgt gcggggggcg ctcgccctcg taccggctgg tggtctacca cgaaatcggc
      721 cacaactggt acatggggca ggtgggatcg aaccaagtgg accgggcatt tttggacgag
      781 gggtttacga cctttatgga gatcaactgc atcgaaacat tccacgggcg gaaagggaat
      841 atcaatttct ttgacaagtg gtatgagaag aaatactacc cgcaggatga agaccgcgcc
      901 gcccgcggct accggcctta ctaccagtgg agcaagtccg gctacgccca gcggatgatc
      961 atctccgccg acaaagcgcc cgagaataac gtctaccgcg tctcatcgta ctacaagacc
     1021 gtggccctgc tcttcaacct ccagtacgtt ttaggcgact cgctcttctg gcaggggatg
     1081 cggacctatt tcgaacggtg gcatttcaag cacccgtacg aggacgattt tcagacggtg
     1141 atggaggagg tttcgggccg ggaactcgac tggtttttcg acgagtggat ccatacgaca
     1201 aagaccatcg actacgctgt cgccaggccg aaaggaaagt gggtcgaaac cgacagcggc
     1261 aaggtctatc gtacgcaact gcgcttatcg cgcaaaggcg agtcaataat gcccctggat
     1321 ttacgcgtga ctctcaaaaa cggccaagcc cgtgatttct tgatacctct cgacgaatct
     1381 gcgaaaccga ctttcgctca gattctgccg atctggccgc aggtgcgtga aattcgaaag
     1441 aagtatgccg ccacggtcga tctcccggct aaaccgaaaa gaattgagat tgacccctcc
     1501 ggacggctgg ccgacgtcaa cccattaaac aaccgctatc ccattcccaa agttgagttg
     1561 caactcgata acctgaagta caaccctcag ccggttgatg cctacctttt gaccgcgcgt
     1621 ccgcaaatct ggttcaattc ccgcgatgcc gtacaactgg gcctccacgt caccggcggt
     1681 tacttaggtt tcacgcgcca gttgagtgcg gaaggggtct cggctacgcg aaggataaaa
     1741 gggcattttg ccgtgaaagc gacccatcct ttcgaggcat tggggcgact cgccttctgg
     1801 tcggtctccg ccagagatta cgacggccgc cggcaattcg cctttgaact ctccaagcaa
     1861 attcgcaatc gctggggtta tccgcccgtg gtggaacacc gtctgatttc cggctttgag
     1921 cgcgcctatg actcccgtta ccttttcgac caatcatctt gggccacgac taccgtgccg
     1981 tacgcagcat acctattctc cggacactgg ccgaaacgct ggggcgcact gcgggtacag
     2041 tttcagaatg agagcggagc aaaaggcaga ttcatttcag atcctgcctt caaccgcact
     2101 tctggtttgg tccgcatcgc ctggaacgtg gtgcccaact ggacactcct ttttcgactc
     2161 tttggctcgc actacatcgg tgcccgggtg ccgcagaagg aatttcggct gaacggcgcc
     2221 gaaccggaac aggtttttga cctgccgctc ctgcggagca aaggaacgat tcccaaccgc
     2281 tggcagaagc actttcgggt tgtcggtggg ggcaatctgc ggggagtgga aaatcctccg
     2341 ctgggagcag aaaatatcat tgcccgcacg atagaagtcc tgccaccgaa ccttgtctct
     2401 ttatctttgc tgcgcaaaat cccgctggct gggaattttc tggccggaat gcgcaactat
     2461 gctttctttg acgacggcat tctcatgttc gacggcgcca aagaccgtta tttctacgat
     2521 ctgggcgtcg gtttcaaatt ccccaacatc tggaaaaacc agaaatttca gatcgacttt
     2581 cccatattcg tcggcagcga ccgccgcttg gagccggtga agttccgctg ggttgtgggg
     2641 tgggaagtag agtaacgggg ggccaggaat cagtagccag gaaaatcaaa tgcaccaaac
     2701 ccagacccgg ccgagaagag cggggggaga ggaaaaagtc cgaaacctaa ttctggaaaa
     2761 gtggtttcat ttaaccaccc gcggaacgaa tgtccgcact gaagtactgg ctggggtgac
     2821 gacgtttctg acaatgtctt atatcatctt cgtcaatccg gcattcttga gtttttctgc
     2881 tgatc
//
LOCUS       DBNGZD010000026        11105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1809957, whole genome shotgun sequence.
ACCESSION   DBNGZD010000026 DBNGZD010000000
VERSION     DBNGZD010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1809957"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="AC16GM_00720"
     CDS             complement(<1..250)
                     /locus_tag="AC16GM_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HIR2922232.1"
                     /translation="MQALLVLEDGRIFRGEGFGAAGKGWGEVVFNTSLMGYQEILSDP
                     SHQGQIVVMTYPLIGNYGLNHEDFESRRPWVQGFVVKEY"
     gene            complement(235..1230)
                     /locus_tag="AC16GM_00725"
     CDS             complement(235..1230)
                     /locus_tag="AC16GM_00725"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR2922233.1"
                     /translation="MAEPTVSQVVVPPTQVQKRLDLFLTHQFPQHSRSYFARLIRSGQ
                     VLVNGVKAKPAQKTRAGDAVTLQLFPQFEPTLEPEPIPLDIVYEDDDLLVINKPAGLV
                     VHPAVGHWTGTLVHALLHHGQSLSQPAPWRPGIVHRLDKDTSGLLVVAKNDFVHARLA
                     EQLKQRTLSRHYLAIVCGHLPKITGMIDLPLGRSRKERKKIVVTRIKSRPAQTLYEVR
                     RQFSLCDLVGVSLLTGRTHQIRAHLSHLGHPVLGDPEYGGRAKWWSGLSGQKRKRAQE
                     LLTFIDRQALHAEKIAFVHPRTEKKLEFSASPPQDFQRVVQSLEKEVESQTCKPS"
     gene            complement(1223..1795)
                     /gene="lspA"
                     /locus_tag="AC16GM_00730"
     CDS             complement(1223..1795)
                     /gene="lspA"
                     /locus_tag="AC16GM_00730"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR2922234.1"
                     /translation="MTTRTVKWPWILVAGVVLFDQLTKQIVKASFTLNESRPVLGDFF
                     RLTYIHNTGGAFGVQLGTPLFYLVSSAMIIGILVLYFFTSRAAIGWSGVSLSLVLGGA
                     IGNLIDRIAYGKVIDFLDFEFFDIAIRPCKLLFWQFPGYQLERWPIFNIADSAVTCGI
                     LLLLATTLVIPRKSPSQVTPVSVTPPAQSG"
     gene            complement(1820..2212)
                     /locus_tag="AC16GM_00735"
     CDS             complement(1820..2212)
                     /locus_tag="AC16GM_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR2922235.1"
                     /translation="MKKKDLEHYEKLLLAKRTELVGEIGSLKKAALDTTIRDSSGDLS
                     GYSYHMADQGTDAMEREKAFLFASKSGRLLYHIDEALRRIKDKSYGVCQECGGEISAA
                     RLEAVPHARLCIKCKEKEEKEKRLEITE"
     gene            complement(2225..5401)
                     /gene="ileS"
                     /locus_tag="AC16GM_00740"
     CDS             complement(2225..5401)
                     /gene="ileS"
                     /locus_tag="AC16GM_00740"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR2922236.1"
                     /translation="MTFVQTASPPRKFKELKDDFKALALEQEVLKFWREEKIFEKSIQ
                     SRTQAPPFVFYEGPPTANGKPGIHHVIARTIKDLICRYKTMQGFRVERKGGWDTHGLP
                     VELEVERELGLNSKARIEAYGVEKFNARCKESVFRYVEDWNALTERIGYWLDLRDAYI
                     TCDNNYIESVWWILAQFHQRGLIYKGHKVVPYCPRCGTGLSSHEVALGYEEITEDSIY
                     VRFRLKKDPKTSILSWTTTPWTLPGNVALAVGEEIDYVKVKQGDEFFVLARARLGILD
                     GNYGIVQEMKGRDLVGLEYEPLYTFLDAGGKKAYYVAAADFVTTEEGTGVVHTAVMYG
                     ADDYRLGEELDLPKVHTVDDDGKFKLEVTLWAGQFVKDAEPSIITDLNSRGLLYKKEK
                     TTHTYPFCWRCESPLLYYARQSWYLKTSQFREQLLKNNRLIHWYPPEIGQGRFGEWLE
                     NNVDWALSRERYWGTPLPIWLCEKCDCQQAVESRSHLQKLSGADTIRLDLHKPFIDQV
                     TFPCPKCRATMRRTSEVIDAWFDSGSMPYAQWHYPFESKEKFKAGFPSDFISEGIDQT
                     RGWFYSLLAISTLLFDQPAYKNVIVIELILDKEGQKMSKTKGNVVDPWEVLNREGADA
                     LRWYLVSTSQPWLPTRFNREEIVEVQRKLLSTLRNVYAFFALYANLDRFDPAQADLKR
                     AVLTELDRWILSRSNSLVREVTESLNGYDITGAARLISDFVIEELSNWYVRRSRRRFW
                     VSGEPKDKRAAYATLHTILLSTVKLCAPFIPFLAEELYRALVCRSAGEPESVHLARFP
                     VVDARWIDSALEARMQAAMQLVALGRAARNKSKVRIRQPLPAMKVVLPGGQAQIDLGS
                     LAEVVQEELNVKQITRVADVEELMTVTAKPNFALLGPKFGQQVKRVAERIRALTTAEL
                     RKLQADGVLTIEHNGARLELDKRDVEITELARGNFAIERNAGYAVALDLTLTEALVDE
                     GLAREVVNRIQNMRKEAGFDVSDRITLSLRTDARLAQAIAAHRQYIQQETLAMLWVEH
                     LADPEFQQAWQINGHPAQIALSRVRQTT"
     gene            complement(5419..6264)
                     /locus_tag="AC16GM_00745"
     CDS             complement(5419..6264)
                     /locus_tag="AC16GM_00745"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR2922237.1"
                     /translation="MQNKVSVNDLKNKINEAVATIRSRTKLAPQIGIVMGTGLGKLGE
                     NIKVETTIDYQDIPHFPLSTVESHAGRLIFGLLEGKPVVAMQGRFHYYEGYTMQQVTF
                     PVRVMKFLGAKVLVVSNACGGLNPVYKSGDLMVINDQINLQGDNPLIGPNDESIGPRF
                     PDMCGCYDRELVALAEKTALELGIKLHKGVYVSVTGPNLETAAEYRFLRIIGADVVGM
                     STVPEVIVARHMDLRVLGFSIITDMGLPDALKPTNLEEIIRVANASEPVLKKLMTETV
                     KRMKV"
     gene            complement(6345..6920)
                     /locus_tag="AC16GM_00750"
     CDS             complement(6345..6920)
                     /locus_tag="AC16GM_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR2922238.1"
                     /translation="MKTTPLDLRSQAFNKKMRGYDPDEVHTFLDAFADELEQHLKEHH
                     LLLEKFGLLESQVAEYRTMENSLRETLMAAQRASEEMRGHARKEADLVLQQAEVESQR
                     MVNETKDQLTQVRKELAELSMLKRNYMLQFRSLLDAHKLMLDDLEKSQAEGTQRTLRL
                     SRKGEVTDEEIEKVVQEFNRSSATRTEREVK"
     gene            complement(6974..7675)
                     /locus_tag="AC16GM_00755"
     CDS             complement(6974..7675)
                     /locus_tag="AC16GM_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR2922239.1"
                     /translation="MNTGPGPIRDNIRLLRERIFAICARLGRSPDEVTVVAVSKGHPA
                     RAIQEALLAGVTDIGENRVQEAEAKKREVAATARWHLVGHLQSNKARKAVALFDVIQS
                     VDTLPLAVKLAGLGAERGKPLRVLIEVKTSREATKFGVASEAVIDFAGKIRELFGLQL
                     EGLMTVGPLTDESDAIRHAFQTLKGLLERINAHLSPPERLAHLSMGMTADYEIALEEG
                     ATLLRIGTAIFGPRP"
     gene            complement(7771..8379)
                     /locus_tag="AC16GM_00760"
     CDS             complement(7771..8379)
                     /locus_tag="AC16GM_00760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/polyprenol kinase family protein"
                     /protein_id="HIR2922240.1"
                     /translation="MDERRPEAAAHEISFANEVKRKLIHLFAAVIPLGYLIVPRTLAV
                     VLLTVAVLVSLTLDVIRIYQLPPRRFVEKMLGSLLRHHESRDFTGGTYILSGALFSVI
                     LFPKPVAVAALGFTVVGDIAAALVGRRYGRVRFGAKSVEGSLACLAACFAVALVVPHL
                     SVWIGAVGALVATIVEATSLPVDDNLSMPLLSGMAMVGLLAF"
     gene            complement(8412..10181)
                     /locus_tag="AC16GM_00765"
     CDS             complement(8412..10181)
                     /locus_tag="AC16GM_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2922241.1"
                     /translation="MKVFASYLNPFFICLLWVGNQPGAQEAKPDPPAVTVDGAPIPFS
                     RFVEKAGALTPKPKTKVEIKESKEHIVNALIVEQIQEGLAAAIPVWDEPAFRQRVEKQ
                     LVQVALGELAGKLLTNAVTVSDSEVRAYFDTSTGQFDIPERVKASHILIAPMKDSSHV
                     DSLKKAQGLFLLTLEAAHGFAERLYGVVARGGAFDSLARLYSDDRLSGVKGGDLGYFA
                     YDEMEEPFAKAAFGMQPGEISRPVQTKYGYHIIKVWDHQPAGKVPLSDSVRTEITRTL
                     QTAKVQIRSQAVIDSLRHAARVTFNDSVIAHSDSVRNSDLWVAIVNERDTVRFAEFWN
                     QLGWLRFGRRPEDSVNFAQKHRLVEQLLSAVLLKQAALITGCFEALSVQAARAQMIAE
                     EQKRRIEKQFQLDYEPSEQELIAYYDAHREEYKEAEPLHVQHIIFKDSSLARSVRDSI
                     VRLGLDFAAMAKKYYPGDPDIRGVAFDLGFISPQAMPKAFFDAASGLGIGEVSQPVKT
                     EWGYHLIKLVNRQTSKSFGAVRSDIAKLLIDRHLAQVRAKRAAEARKEHKIKVNKKLI
                     DAYQFAPEAPAAQAGAGQPAGSR"
     gene            complement(10197..>11105)
                     /locus_tag="AC16GM_00770"
     CDS             complement(10197..>11105)
                     /locus_tag="AC16GM_00770"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2922242.1"
                     /translation="TSLFAAKFAGEFLRLWVGARPLGMGSAFVAVADDITAGYWNPAG
                     LAALSKREAMFMHAETFGSLLNHDYLAFGLPVALGHGVGAASFIRLGGGGLKLTSLPD
                     PTRPISLTNRPFVSRVASHGDYALFFSYGLQRWPKIRLGASGKVLFRDLVDNSAFGLG
                     IDLAAQAQVNRWVSAGITVQDATTSFLAYDTGTKESITPTVKTGLAGRQMFGEITMTG
                     AVDADFNFDNFRDAAQFWMGAISADTHFGLEADYRGIGQVRIGSDIGKFSAGAGLRWR
                     GWRLDVAYLNHPQLDNSYRISLAATW"
BASE COUNT         2251 a         3289 c         3174 g         2391 t
ORIGIN      
        1 agtattcttt gaccacaaac ccctgcaccc agggccgccg cgattcaaaa tcctcgtggt
       61 tcaaaccgta gttgccgatc aaaggatagg tcatgacgac aatctggccc tggtgcgagg
      121 ggtcggagag gatctcttgg taacccatga gactggtgtt aaaaacgacc tcaccccacc
      181 ctttcccggc ggcaccgaag ccctcgccgc ggaaaattct gccgtcttct aatactaaga
      241 gggcttgcat gtttggcttt ctacttcttt ttccaacgac tgtacgaccc gctgaaaatc
      301 ttgcggtggc gaagcggaaa attccaattt tttttctgtc cggggatgca cgaaagcaat
      361 tttctccgca tggagcgctt gccggtcgat gaaggtgagc aactcctgcg cccgtttcct
      421 tttttgtccg gacagacccg accaccactt ggcccggccg ccatattcgg ggtcacccaa
      481 caccggatgc ccgaggtggg agaggtgcgc gcggatctgg tgcgtccggc cggtcaaaag
      541 cgaaacgccg accaggtcgc agagggaaaa ctgccggcgt acctcgtaga gggtctgcgc
      601 cgggcggctt ttgatcctgg tcaccacgat ttttttacgc tctttccggg aacgaccgag
      661 aggtaaatct atcatcccgg ttatttttgg caagtgcccg cagacgatgg ccaggtagtg
      721 gcgggaaagt gtccgctgtt tgagttgctc agccaggcgg gcgtgaacaa aatcattttt
      781 ggctaccacc agaaggccgg aggtgtcttt atccagccgg tgaacgatcc ccggccgcca
      841 gggggccggt tgcgacagac tctgaccgtg gtggagcagg gcgtgaacga gggttccggt
      901 ccagtgaccg accgccggat gtacgaccaa tcccgccggt ttgttgatga ctaaaaggtc
      961 atcgtcttca tagacaatgt ccaaaggaat cggctccggc tccagcgttg gctcgaattg
     1021 ggggaacaac tggagagtga ccgcatcacc ggcccgggtc ttttgcgccg gcttggcttt
     1081 gaccccgttg accagcacct ggccggaacg aatcagccgg gcgaagtagg agcgggaatg
     1141 ctgcggaaat tggtgggtta aaaaaagatc gaggcgcttc tggacctgtg tgggcggaac
     1201 gacaacctgg gaaacagtcg gctcagccac tttgggcggg tggggtcacg gacacaggtg
     1261 ttacctgact cggcgatttc ctgggaataa cgagagtggt ggcgagcaaa agcaggatgc
     1321 cgcaggttac cgctgagtcg gcaatgttga aaatcggcca gcgttccaac tggtagccgg
     1381 ggaactgcca aaagaggagt ttgcatggcc ggatggcaat gtcgaagaac tcaaagtcca
     1441 gaaaatcaat cacttttcca taggcaatcc ggtcgataag gtttccgatg gcgccgccga
     1501 ggaccagtga caggctcacc ccactccagc caatggcggc ccgggatgtg aaaaagtaga
     1561 gcacgagaat accgatgatc attgccgagg agaccaggta aaaaagagga gtgccgagtt
     1621 gcacgccgaa agcgccgccg gtgttgtgga tgtaggtcaa gcgaaaaaaa tcacccagaa
     1681 ccggtcgtga ttcattcagc gtaaaactgg ccttcactat ctgtttggtc agttgatcga
     1741 agaggacaac gcccgcaacg agaatccagg gccattttac cgttctcgta gtcacggagc
     1801 cgctccggca cggagaacct tactccgtta tctccagccg tttttctttt tcttcttttt
     1861 ctttacactt gatgcagagc cgtgcgtggg gaacggcttc cagccgcgcc gccgaaattt
     1921 ccccgccgca ttcctgacag acgccgtagg atttgtcctt gatgcgccgc agggcttcat
     1981 cgatgtggta gaggagcctt ccgctcttgg aagcgaaaag gaacgctttc tcccgctcca
     2041 tggcgtcggt tccctggtcg gccatgtggt aggagtaacc cgagaggtcg ccggaggagt
     2101 cgcgaatggt ggtatccagg gccgctttct tgagggaccc gatttccccc accagttcgg
     2161 tccgtttggc taaaagtaat ttttcgtaat gttcgagatc tttctttttc atcgtctctg
     2221 ctccctatgt tgtctgccgc acccgggaga gcgcaatctg ggcggggtgg ccgttgatct
     2281 gccacgcctg ttggaactcg ggatcggcca gatgctccac ccaaagcatg gcgagcgttt
     2341 cttgctgtat atactggcgg tgggcggcaa tcgcttgcgc caatcttgcg tcggtgcgga
     2401 gcgaaagcgt aatccggtcg gacacgtcga atcccgcctc tttgcgcatg ttctgaatcc
     2461 ggttgaccac ttcccgtgcc agcccttcat ccaccagcgc ttcggtgagg gtcaaatcca
     2521 gtgccacggc gtacccggca ttgcgctcga tggcaaaatt ccctcgggcc agttcggtga
     2581 tttccacgtc ccttttgtcc agttccagcc tggcgccatt gtgttcaatg gtcagaacgc
     2641 cgtcggcctg caacttccgg agctcggccg tggtgagggc ccggatacgc tcggccactc
     2701 gcttcacctg ttggccaaac ttggggccca ggagcgcaaa atttggcttg gcggttacgg
     2761 tcattagttc ctctacatcg gcaactcggg taatctgctt aacgttcaac tcttcttgca
     2821 ccacctcggc caaactgccg agatcgattt gtgcctggcc gccgggcagc accaccttca
     2881 ttgccgggag gggctgacga attctcacct tacttttgtt cctggccgcc cgccccaagg
     2941 ccactaattg catagccgcc tgcatccggg cttccagtgc gctgtcaatc cagcgggcat
     3001 ccacgactgg aaaccgtgcc agatggacgc tctccggctc accggcgctc cggcacacga
     3061 gcgcccggta caactcctcg gctaaaaagg gaatgaaggg cgcgcagagc ttgacagtgg
     3121 agagaagaat tgtatggagc gtcgcgtagg ccgcccgttt atccttcggc tccccggaga
     3181 cccagaagcg gcggcgcgag cgccgcacgt accagttgga gagttcttca atcacaaagt
     3241 cggaaatgag ccgcgcggcc ccggtgatgt catagccgtt cagactctcc gtcacctctc
     3301 gtacaagcga gtttgagcgg gagagaatcc agcgatccag ttcggtgagc accgcccgtt
     3361 tgaggtctgc ttgggctgga tcgaaccggt ctaagttggc gtaaagggcg aagaaggcat
     3421 agacgttgcg caaggtcgaa agaagcttgc gctggacttc gacgatttct tcccggttga
     3481 agcgggtcgg cagccagggc tggctggtcg aaaccaggta ccagcggagg gcgtcggctc
     3541 cttcgcggtt caaaacctcc cacgggtcga ccacgtttcc cttcgtcttc gacatcttct
     3601 ggccttcttt atccagaatc agttcgatga caatcacatt cttgtaggcc ggctggtcaa
     3661 aaaggagcgt cgagatagcc agaagtgaat aaaaccagcc tcgcgtctgg tcgatcccct
     3721 cgctgatgaa gtcggacggg aaaccggctt tgaatttctc tttactctcg aacgggtagt
     3781 gccactgggc atacggcatc gacccggaat cgaaccaggc gtcgattact tccgatgtgc
     3841 ggcgcattgt cgcccgacac ttggggcagg ggaaagtaac ctgatcgatg aacggcttgt
     3901 ggagatcgag gcgaatcgta tctgctccag aaagtttttg caagtgactg cgcgattcga
     3961 cagcttgttg gcaatcgcac ttctcgcaga gccagatcgg caagggtgtg ccccagtacc
     4021 gctcgcggga aagggcccag tcgacgttgt tctccagcca ctcgccgaaa cggccttggc
     4081 cgatctccgg cgggtaccag tggatcagcc ggttattttt caacagttgt tcgcggaact
     4141 ggctggtttt gaggtaccag gactgccggg cgtagtagag caaaggcgac tcgcagcgcc
     4201 agcagaaggg gtaggtgtgc gtggtttttt cctttttgta gaggagcccc cgcgagttga
     4261 gatcggtaat gattgacggc tccgcatcct tgacgaactg cccggcccat agggtcactt
     4321 ccaacttgaa tttcccgtcg tcgtcgaccg tgtggacttt aggcaaatcg agctcttcgc
     4381 ctaaacggta gtcatccgcc ccatacatta ctgccgtgtg gacgacgccc gtgccttcct
     4441 cggtggtgac gaaatcggcc gcagcgacgt agtaggcttt tttgcctcct gcatccaaga
     4501 aggtatagag cggttcgtac tccaaaccga cgaggtcgcg tcctttcatt tcctgcacga
     4561 ttccgtagtt gccatcgaga atacccaaac gcgcccgggc caagacaaaa aattcatccc
     4621 cctgtttgac cttgacgtag tcgatctcct cacccaccgc cagagcgacg ttccccggca
     4681 aagtccaggg cgtcgtcgtc caggagagaa tcgaggtttt tgggtccttt ttaagccgga
     4741 aacggacgta gatcgaatct tcggtgattt cttcatatcc cagagccact tcgtggctgg
     4801 aaaggccggt tccgcagcgc gggcagtagg ggacgacttt atggcctttg tagatgagac
     4861 cgcgctggtg aaattgggcc agaatccacc agaccgactc tatatagtta ttgtcgcagg
     4921 tgatgtaggc atcgcgcaag tccagccagt agccgatccg ctcggtcagg gcattccagt
     4981 cctcgacgta gcggaagacc gattccttgc agcgggcgtt gaacttttcg accccgtacg
     5041 cttcaatgcg ggctttggaa ttcaagccca gttcgcgttc cacttccaac tccaccggca
     5101 aaccgtgggt gtcccagccc cctttgcgct ccacgcggaa cccctgcatc gtcttgtagc
     5161 ggcagattaa atctttgatc gtccgggcga tgacgtggtg gatgcccggc ttcccgttgg
     5221 cggtcggcgg cccttcgtag aagacgaaag gcggtgcctg cgtccggctc tggatgctct
     5281 tttcaaaaat tttctcctcc cgccagaact tgagcacttc ctgctccagc gccagggcct
     5341 tgaagtcgtc tttgagctct ttaaatttcc tcggaggtga ggcggtttgc acaaacgtca
     5401 taagaaataa ttacactctc agactttcat tcgcttgacc gtttcggtca tcaatttctt
     5461 cagcaccggc tcggaagcgt tggctacccg gataatttcc tcgaggttcg tcggcttgag
     5521 cgcgtcgggc aagcccatat cggtgatgat ggagaacccg agaacgcgca gatccatatg
     5581 ccgcgccacg atcacctcgg gaacagtgga cattcccacg acatcagcac cgatgatccg
     5641 taagaaccgg tactcggcgg cggtctccag attcggcccg gttacagata cgtaaacgcc
     5701 cttgtgcagt ttgattccca actccagcgc ggtcttctcg gccagtgcga ccagttcccg
     5761 gtcgtagcag ccgcacatat cggggaaacg cgggccgatc gattcgtcat tcggcccgat
     5821 gagcggatta tccccctgca ggttgatctg gtcattgatg accataaggt cgccggactt
     5881 gtagaccgga ttgagcccgc cgcaggcgtt ggagacaacc agcaccttgg ctcccagaaa
     5941 tttcatcacc cgcaccggga aggtgacctg ctgcatcgtg taaccttcgt agtagtggaa
     6001 gcgtccctgc atggccacga ccggtttgcc ttccaggagc ccgaagatca gccgcccggc
     6061 gtggctttcc accgtggaga gcgggaaatg cggaatgtcc tgatagtcga tggtcgtttc
     6121 gaccttgatg ttttcgccca gcttccccag cccggtgccc atgacaatgc cgatctgtgg
     6181 cgccagtttc gtccggctgc ggatggtcgc taccgcttcg tttattttat tcttgagatc
     6241 attcaccgat accttgttct gcatagaagt ccttgtctgt gttgatatcc gtaggtagtc
     6301 cggctcgtcc cggagccgga caccttgcgt tttcacctaa ccacctactt cacttcccgc
     6361 tcggtacgag tcgcactcga ccggttgaat tcctgtacca ccttttcgat ttcttcgtca
     6421 gtgacctcgc ctttacgcga caggcgcagg gtgcgctggg tcccttcggc ttgcgatttt
     6481 tccaggtcat ccagcatcaa tttgtgcgcg tccagaagcg agcggaactg cagcatgtag
     6541 ttgcgcttga gcatggagag ctcggctaat tctttccgca cctgggtcaa ctggtctttg
     6601 gtctcgttca ccatccgctg cgattcgacc tccgcctgct ggaggaccag gtcggcctcc
     6661 ttcctggcgt ggccgcgcat ctcttcggag gcgcgctggg ccgccatcag ggtctcccgc
     6721 agggaatttt ccatcgtccg gtactccgcc acctgggact ccaaaagccc gaatttctcc
     6781 agaagcaggt ggtgctcctt caagtgctgt tccagttcgt cggcaaaagc atccagaaag
     6841 gtgtggacct cgtccgggtc atacccgcgc atttttttgt tgaaggcctg gctgcgcaga
     6901 tccaaaggcg tggttttcat cgtcggctcc ttttcctgtc ctcaaccgga accaccggac
     6961 aaccttccca ccgtcacggg cgcggcccga agatcgccgt cccgatccgt aagagcgtgg
     7021 cgccttcttc gagggcgatc tcgtagtcgg cggtcatccc catcgacagg tgcgccagcc
     7081 gctccggggg actcaggtgt gcgttgatcc gttccaaaag acccttgagc gtttgaaatg
     7141 cgtggcggat tgcgtcgctt tcatccgtca gcgggccgac ggtcatcagt ccttccaatt
     7201 gcaggccgaa aagttcacgg atctttccgg cgaaatcgat caccgcttcg gatgccacgc
     7261 cgaacttggt ggcctcgcgc gacgtcttca cctcaatgag tacccgcagg ggcttcccgc
     7321 gctcggcgcc cagcccggct aactttaccg ctaatggcag cgtgtctacc gactgaatga
     7381 cgtcaaaaag tgcgaccgcc ttccgcgcct tattgctctg cagatgtccg accaagtgcc
     7441 agcgcgcagt tgccgcgacc tcccgctttt tcgcttccgc ctcctgcacc cgattctcgc
     7501 cgatatcggt tacgccggct aaaagcgctt cctgaattgc ccgggccggg tggcctttgc
     7561 tcacggccac taccgttacc tcatccggac tacgacccaa ccgcgcgcag atagcaaaaa
     7621 tccgctcccg caggagccgg atattgtcgc gaatcggccc cggaccagtg ttcatccctc
     7681 accaacctgg cccatgcgga gggagatatt acaccgggaa aggccaaaat gcaacagccg
     7741 atgcggcccg cctttctgca gggagaggat ttaaaaggcc aaaagaccga ccatcgccat
     7801 tccagacaaa agcggcatcg agagattatc atccaccggc aggctggtcg cctccacaat
     7861 ggttgccacg agagcaccga cggcaccgat ccagaccgag aggtggggaa cgaccagggc
     7921 aacggcgaaa caggccgcca ggcacgccag actgccttcc accgatttcg ccccgaaccg
     7981 cacccgcccg taccggcgac ccaccagagc cgccgcaata tctcccacga cggtgaaccc
     8041 gagggcggca accgccaccg gcttgggaaa aaggatgaca gagaaaagcg ccccggacaa
     8101 tatgtacgtg ccaccggtaa agtcgcggct ctcatggtgg cgcaaaagcg atcccagcat
     8161 cttttcgacg aaacgtcgcg gcggcagttg gtagatgcga atgacgtcca acgtgagcga
     8221 aaccaaaacg gcaacagtta aaaggacgac ggcgagcgta cgtggtacga tgaggtagcc
     8281 caggggaatg acggccgcaa acaggtgaat cagtttgcgc ttgacttcat tggcaaagga
     8341 tatctcgtga gcggctgctt ccggtctccg ttcgtccacg gtgggaggca gaagtgtgct
     8401 tcggcggtga cctaccggga gccggcgggc tgacccgcgc cagcttgggc tgctggcgct
     8461 tccggcgcaa attggtaggc gtcgatcagt ttcttattca ccttgatctt gtgctctttg
     8521 cgcgcctccg ccgcccgctt ggcccggact tgcgccaggt gccggtcgat gagaagtttg
     8581 gcaatgtcag accgtactgc gccgaagctt ttgctcgtct gccggttcac caatttgatc
     8641 aggtggtacc cccattccgt tttgactggt tggctcactt cgccaatccc cagccccgag
     8701 gcggcatcga agaacgcttt tggcattgcc tgcggggaaa tgaagcccaa gtcgaaagcc
     8761 actcctcgga tgtcgggatc gccgggataa tacttcttgg ccatcgcggc aaaatccaag
     8821 cccagacgga cgatcgagtc gcgcaccgat cgggccaggg acgagtcctt gaaaataatg
     8881 tgctggacat ggagcggctc ggcctctttg tactcttccc ggtgggcatc atagtaggca
     8941 atcagttcct gttccgaggg ctcgtagtcg agctggaact gcttttcaat ccgccgtttc
     9001 tgttcctcag cgatcatctg ggcccgggcg gcttggacgc tgagcgcctc gaagcacccg
     9061 gtgatcagtg ccgcctgctt caggagcacc gccgaaagta actgctccac cagccggtgc
     9121 ttctgggcga aattgacgct gtcctccggg cgtcgcccga agcgtaacca gccgagctga
     9181 ttccagaact ctgcaaaacg cacggtgtcc cgttcattga cgatagccac ccagagatcg
     9241 gagttccgga ccgaatcact gtgggcgatg accgaatcgt tgaacgtcac ccgggcagcg
     9301 tggcgcaagc tgtcgattac tgcttgtgaa cggatttgaa ccttggcggt ttgtaacgtc
     9361 cgggtgatct cagtgcgtac ggagtcgctc aagggcacct tgcccgccgg ctggtgatcc
     9421 cacactttga tgatgtggta gccgtacttc gtttggaccg gccgggagat ctccccgggc
     9481 tgcatcccga aggccgcttt cgcaaacggc tcttccatct cgtcataagc gaagtagccg
     9541 aggtcgcctc ctttgacccc gctcagccga tcgtccgagt acaaacgcgc cagggaatca
     9601 aaggcgcctc cccgggcaac caccccatac aagcgttcag cgaagccgtg tgccgcctca
     9661 agggtgagga ggaacaagcc ctgggccttt ttgagtgaat cgacgtgact ggaatccttc
     9721 atcggggcaa tcaggatgtg gctggccttc acccgttcgg ggatatcgaa ctggccggta
     9781 ctggtatcga agtaggctcg cacctcgctg tcggaaacgg tgacggcgtt cgtgagcaac
     9841 ttcccggcta gttctcctaa cgcgacctgg actaactgtt tttccactcg ctggcgaaat
     9901 gccggttcat cccagaccgg aatagccgcc gctaaaccct cttgaatttg ctcgacaatc
     9961 agtgcattga cgatgtgctc tttactctct ttaatttcga ctttggtttt tggcttgggc
    10021 gtaagcgccc cggctttctc cacaaagcgg gagaagggga tcggcgcgcc gtcgacggtg
    10081 acggccggtg gatccggctt ggcttcttgt gcacccggct gattgcctac ccataagagg
    10141 cagataaaaa atggattcaa gtacgacgcg aaaactttca ttgcatttcc ctcatttcac
    10201 caggtggctg ccagagaaat cctataagag ttatctaact gcggatggtt gaggtacgcg
    10261 acatccagcc gccacccgcg ccagcgcagg ccggcgcccg cgctgaactt gccaatgtct
    10321 gagccaatcc gaacctgccc gatcccgcga tagtcggctt ccaacccgaa gtgcgtgtcc
    10381 gccgagattg cgcccatcca gaactgcgcg gcgtcccgga agttatcaaa gttgaagtcg
    10441 gcgtcaacgg caccggtcat cgttatttct ccgaacatct gccgaccggc aagcccggtc
    10501 ttcaccgtcg gcgtgatgct ttccttggtg ccggtatcgt aggcgagaaa ggaagtagta
    10561 gcatcctgaa ccgttatccc cgcgctcacc cagcggttca cctgcgcttg ggcggccaga
    10621 tcgatcccca aaccgaaagc ggagttatcg accagatcgc ggaagagcac tttgcccgaa
    10681 gcgccgagcc gaattttggg ccagcgttgc agtccgtagg agaagaaaag agcatagtcg
    10741 ccgtgggaag ccacccggga gacaaacggc cggttggtga gagaaatcgg acgcgtggga
    10801 tcgggcaggg aggtaagttt caacccccct ccgcccagac ggatgaaact ggccgccccg
    10861 acgccatggc cgagtgccac cggcagcccg aaggccaggt aatcatggtt cagaagcgaa
    10921 ccaaacgtct ccgcatgcat gaacatcgcc tcgcgcttcg acagggcggc caatccggcc
    10981 gggttccagt aaccggcggt gatgtcatcg gccaccgcca caaaggcgct gcccatgccc
    11041 aaaggccgcg ctccgaccca caggcgcagg aactccccgg caaatttggc cgcgaaaagt
    11101 gaggt
//
LOCUS       DBNGZD010000027         5980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_351394, whole genome shotgun sequence.
ACCESSION   DBNGZD010000027 DBNGZD010000000
VERSION     DBNGZD010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_351394"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="AC16GM_00775"
     CDS             complement(<1..1075)
                     /locus_tag="AC16GM_00775"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR2922243.1"
                     /translation="MDLFEKCRRFTEARKVMAAGYYPYFRPISSSLGTEVVVKGQKMI
                     MIGSNNYLGLTNHPKVKAAAEAAVEKYGSGCTGSRFLNGTLDIHEELESRLAAFMRKE
                     AGLVFSTGFQTNLGTISAIVQKGEYLIIDRSVHASIVDGCRLAFGKTIKYRHNDMDDL
                     KRVLASLGKGTGKLIVVDGVFSMEGDIAKLPEICDLAHRYNARTLVDDAHSIGVLGPG
                     GRGTAEHFGLEDEVDIIMGTFSKSFASLGGFIVAKEEVIHYIKHFSRELIFSASMPPA
                     AVAACLAALDIIETEPQRRARLWEITRRVHRELRGLGFDIGVTETPIVPIYIGADMRT
                     FQFWKALTDAGVFSNPIVSPAVPK"
     gene            1195..2175
                     /locus_tag="AC16GM_00780"
     CDS             1195..2175
                     /locus_tag="AC16GM_00780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR2922244.1"
                     /translation="MRALVTGATGFVGHHLAALLVARGYTVRVLLRPQSEHKIAEELS
                     VEPVFGGLADRPSLISAVAGCDLVFHCAGLVKAAHPEDFFAVNHEGTQHLLEAVLASR
                     QKLSRFVYVSSVAAGGPSLNGQPLDETVPVAPVSPYGRSKLAGERAVLGLKEKIPVTI
                     IRPPAVFGPRDKQMLFFFQMVKRGWIPRFSRDDRRLSLIYVGNLVQGIIQAAESPRAV
                     GEVFFLADGRTYVTSEVVHVIAAALRVKVKQVYVPGPVLYTLAAAAELWGNITGRPPL
                     LWRGRARDLSRYLWIVNIEKARRAFGYAPDVPLEESVRETCAWYQRAGWL"
     gene            2202..3371
                     /locus_tag="AC16GM_00785"
     CDS             2202..3371
                     /locus_tag="AC16GM_00785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR2922245.1"
                     /translation="MTERLATGMAKYRREFPFTRHTVFLNTAAFGPLPLRIKKMLDRF
                     DRTMLELDDWEYDEKTFHLLDGSRALAARLVNARSDQVGFSFNTSFGLNICAAGLPWH
                     PGDEVLLSGVDFPANIYTWSNLKRRGVAIRFLSRQGRDCIDPEQVEQSIAPRTKLLAL
                     SFVQYFNGYKNDLETLGEICRARNVWLVVDGTQGVGAEMLDVRKAQIDFLACGGQKWL
                     LSAPGTGFFYISDRLREVLEPIFFSWLGVDWGLDFSDLQKHDLRPFASARRFEIGSYP
                     ARLVRHFHAALELILEAGVARIHEHNHALLDRLLAYLADSPYRLKSPWSEERRSSIVS
                     FASRDNRRLLQFLRKRKISVALRERGIRVSPHFFNNAADMDSLIGALKQFQAKSR"
     gene            3413..3988
                     /locus_tag="AC16GM_00790"
     CDS             3413..3988
                     /locus_tag="AC16GM_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922246.1"
                     /translation="MAKKYLLALLGLGMVFATGCARRVVVVERQTPPPPAARHRGQAV
                     ASQEHLENGKRMFHRGRYRQAAHQFEIAIAEDPNNWEAHWWLGLTLREMRDYDDCLDH
                     FRVAIRLHDDPDWVARVRVDVGLVWELRGDRDKALVEYDLALRARPGYADAIKARERL
                     KERREALKEREDDKDKGHRGNKGKRRSERDD"
     gene            complement(4020..4679)
                     /locus_tag="AC16GM_00795"
     CDS             complement(4020..4679)
                     /locus_tag="AC16GM_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922247.1"
                     /translation="MQILEITFGRFPEIIRLNPAFRTGAKQVRVLLRVESNGVDYRSR
                     FAAVFSGLTRWFPTLAHHDCCQWGDHAPQIQTDGIPLKTTGGLADVAHLVEHLMVDLH
                     CRVGGMRRCSGVTCGYRKSADRFDLFVECPEHRLGRFSAGFATFLVRQLFQRRKVSRG
                     YRRVLALARYLLAHPRAPLDRQKLSRTMGWTQSQTRFAFRKLAEFQFLENGSTNGGQK
                     K"
     gene            complement(4816..5589)
                     /locus_tag="AC16GM_00800"
     CDS             complement(4816..5589)
                     /locus_tag="AC16GM_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922248.1"
                     /translation="MVKYFATLLACAALLLAGCSQKQTEQTPEETPQMSQADQGAETQ
                     AAPEKQTNTQASNKTMKKSSRTAGQQAPKAEEAKPVVLEVPAGMQLVVALSDSINSGK
                     NKTGEKFDATLAKPIVVNGQTVIEEGAPITGEIILARGSSGLKSTAELVLTLNEIEAH
                     GQAYHVTTTTVEAKEKSKTGRNVGMIGGGAAVGTAIGAIAGKKKGAIIGGVAGAAAGT
                     GAAVLTGKKNLLYPAGSELTFILQQPMKVTVKKSALAAH"
     gene            complement(5762..>5980)
                     /locus_tag="AC16GM_00805"
     CDS             complement(5762..>5980)
                     /locus_tag="AC16GM_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922249.1"
                     /translation="VCTPAFSPSENPVLATVAAWTPQSSCHATNISIWWLEWDFTLRY
                     SPNPYSPTITGGWFRLGARLFGIEARKE"
BASE COUNT         1187 a         1665 c         1815 g         1313 t
ORIGIN      
        1 ctttgggcac ggcgggggaa acgatcggat tgctgaatac gcccgcatcg gttaacgcct
       61 tccagaattg gaacgtgcgc atatccgcac cgatgtaaat cggcacaatg ggggtctcgg
      121 tcaccccgat gtcaaacccc agcccccgca gttcgcggtg aacccggcgg gtgatttccc
      181 acagccgggc gcgcctctgg ggctcggtct cgataatgtc taacgccgcc aggcaggccg
      241 ccaccgcggc cggcggcatg ctggcagaga agatcaattc ccgtgagaag tgcttgatgt
      301 agtggatgac ttcttcttta gccacgataa agccgcctag ggaggcgaac gatttgctga
      361 aagttcccat gatgatgtcg acctcatctt ccaggccgaa gtgttcggcg gtgccccggc
      421 cgccggggcc caaaacgcca atcgagtgag cgtcgtccac cagggtacgg gcgttgtacc
      481 ggtgcgccag gtcgcagatt tccggcagtt tggcgatgtc gccctccata ctgaagacgc
      541 catcgaccac gatcaatttc ccggtgccct tgcccaaaga ggccagcacc cgcttgaggt
      601 cgtccatgtc gttgtgccgg tacttgatcg tcttgccgaa ggccagccgg cagccgtcta
      661 caatcgaagc atggaccgag cggtcgataa tgaggtactc gcccttttgg acgatggctg
      721 aaatagtgcc gaggttggtc tggaagccgg tggaaaagac cagcccggcc tctttgcgca
      781 taaaggcggc caggcgggac tctaattctt cgtgaatgtc cagggtgccg ttcaaaaagc
      841 gtgaaccggt gcagcccgaa ccgtattttt ccaccgcggc ctcggcggcg gctttgactt
      901 tgggatgatt ggtaagcccc aggtagttat tggagccgat cataatcatc ttctggcctt
      961 tgaccaccac ttcggtcccc agagaagagg aaataggccg gaagtaggga tagtagcctg
     1021 cggccatgac tttgcgcgcc tcggtaaaac ggcggcattt ttcaaataag tccataagac
     1081 cttaggtatt taggtgaaat tgtgtacgct ggtctgaaac cccgcctttt catctcgcca
     1141 aagggtaata tccgacgatt tgcctgtcaa gagcaatatg cgttacttga gattgtgcgc
     1201 gcgctggtaa cgggtgccac cggatttgtg ggacatcacc ttgcggctct actggttgcc
     1261 cgggggtaca ccgtgcgggt acttttgcgt ccccaaagcg agcataaaat cgccgaagaa
     1321 ctttcggttg agccggtttt tggcggtctg gcggatcggc ccagtctcat ctcggcggtt
     1381 gccggctgtg acctcgtatt ccactgtgcc ggactggtca aggccgctca tccggaagat
     1441 ttctttgccg tgaatcacga gggcacgcag caccttttgg aagcggtgct cgcttcccgg
     1501 cagaaactgt cacgcttcgt ctacgtttcc agtgtcgcgg ccggcgggcc gagtttgaac
     1561 ggccagccgc tggatgagac cgttcctgtt gcgccggttt cgccctatgg caggtcgaaa
     1621 ttggccgggg aacgggcggt cctggggctc aaagaaaaaa ttccggtgac catcattcgc
     1681 cccccggcag tgttcggacc acgggataag cagatgctct ttttcttcca gatggtgaaa
     1741 agaggttgga ttccccgctt cagccgggat gaccggcggt taagtttgat ctacgtggga
     1801 aatctcgtgc aggggattat ccaggcggcg gaaagcccca gggccgtggg cgaagttttt
     1861 tttctggcgg acggccggac gtatgttacc agcgaagtgg ttcacgtcat tgccgccgcc
     1921 ttgcgggtga aggtgaagca agtctacgta cccggaccgg tactctatac tctggcggcg
     1981 gctgccgaac tgtgggggaa cataacggga aggccgccgc tattatggcg cgggcgggcc
     2041 cgcgaccttt cgcgctatct ttggatagta aacatagaga aagcgcgccg ggcctttggc
     2101 tacgcgccgg acgttccctt agaggaaagt gttcgcgaaa cctgtgcgtg gtaccagagg
     2161 gcgggatggt tgtagagatc aggtcaagta agggaaggag agtgacagaa cggttggcaa
     2221 ccgggatggc gaaataccgg agagaatttc cgttcacacg acataccgtt tttctgaata
     2281 ctgcggcttt tggcccgctg ccgctgcgga tcaaaaagat gctcgaccgc ttcgaccgca
     2341 ccatgctcga gctcgatgat tgggagtacg acgaaaagac ctttcattta ctcgacggct
     2401 cccgcgctct ggccgcgcgt ctcgtcaatg cccgttctga ccaggtaggc ttcagtttta
     2461 acacttcctt tggcttgaat atctgcgccg ccggcctgcc ctggcacccg ggggacgaag
     2521 ttttgctctc cggcgttgat tttcccgcca atatctatac ctggtccaat ctgaagcggc
     2581 ggggagtcgc tatccggttt ttgtcccgcc aagggcggga ctgcatcgat ccggagcagg
     2641 tcgaacaatc aattgccccc cgaacgaaat tgctggcgtt aagttttgtc cagtatttca
     2701 acgggtacaa aaacgatttg gaaactttgg gagaaatctg tcgggcccgg aacgtctggc
     2761 tggtcgttga cggcacccag ggggtgggcg ccgaaatgct ggacgtccgg aaggcgcaga
     2821 tcgatttcct ggcctgcggc ggccagaagt ggctcctttc cgctcccggc accggttttt
     2881 tttacatctc cgaccgtttg agggaagtgc tggagccgat ctttttcagc tggctggggg
     2941 tggactgggg actcgacttt tccgacctcc agaagcatga tcttcgcccc ttcgcctcag
     3001 cccggcgctt tgaaatcggc tcttacccgg cgcggctggt gcggcatttc cacgcggctt
     3061 tggaactgat tctggaagcc ggagtcgccc gcatccacga acacaaccac gccctgctgg
     3121 accgcctgct ggcctatctg gccgactcgc cctaccggct caaaagtccc tggtcggaag
     3181 agcgccgttc cagcattgtt tcgtttgcga gtcgggacaa tcggcgtctg ttgcagttcc
     3241 tcaggaaaag gaagatttcc gtggccctgc gagaaagggg cattcgcgtt tccccgcact
     3301 tctttaataa tgcagccgac atggatagcc tgattggagc gcttaaacaa tttcaggcaa
     3361 aaagccgata gtagggttgg tagtgatgca accgactcac aaggagggga ggatggcaaa
     3421 gaagtatctt ttggcccttt tggggctggg tatggtattc gccaccggct gcgcccggcg
     3481 ggtggtagtg gtggagcgtc aaacaccccc gccgccggca gccaggcacc gggggcaggc
     3541 ggtcgcctcg caggaacacc tggagaatgg gaagcggatg ttccaccgcg gccgctaccg
     3601 ccaggcggcg caccagttcg aaatagcgat tgcggaagac ccaaacaact gggaagccca
     3661 ctggtggctg ggtttgactt tgcgcgaaat gcgcgactac gacgactgtc tggatcactt
     3721 ccgggttgcc atccggctgc acgacgatcc cgactgggtg gcccgggtgc gggtggacgt
     3781 cggtctggtc tgggagctgc gcggcgaccg ggataaggcc ttagttgagt acgacctggc
     3841 actgcgggcg cggcccggct atgcggatgc catcaaagcc agggagcggc tcaaagagcg
     3901 ccgggaagcc ttgaaagagc gcgaggacga caaggacaaa ggacaccggg gtaacaaggg
     3961 gaagcgtcgc agcgagcgcg acgattgagg ccaaaaggcc ccgccttggc ggggcctttt
     4021 catttctttt ggccgccgtt ggttgacccg ttctccagaa attgaaattc cgccaatttc
     4081 cggaacgcaa agcgggtctg gctttgggtc cagcccattg tgcgcgagag cttttgccgg
     4141 tcgagcggcg cccgcgggtg ggccaagagg tagcgggcta aagcgagcac gcgccggtag
     4201 ccccgcgaaa cttttcggcg ctggaagagt tgccgtacca aaaacgtcgc gaaaccggcg
     4261 ctgaaccgcc ccagccggtg ctccggacat tccacaaaaa ggtcaaagcg gtcggccgac
     4321 ttccggtagc cgcaggtgac cccgctgcac ctccgcatcc cgcccacccg gcagtggagg
     4381 tcaaccatca ggtgctcgac caggtgggcg acatccgcca gaccgccggt tgtttttagc
     4441 ggtatgccat cggtttggat ttgcggcgcg tgatcgcccc actgacagca gtcgtggtgc
     4501 gcgagagtcg gaaaccagcg ggtcaagccc gagaaaaccg cagcgaagcg gctgcggtag
     4561 tcaacgccgt tggattctac ccgtaaaagt acccgcacct gtttggcgcc ggtgcggaaa
     4621 gcggggttga gccggataat ttccgggaac cggccgaagg tgatttccag aatttgcatg
     4681 gtcttttgtc ggacaggcgt actccttttt gtatcggcca atggagaaag caacttgacg
     4741 gaaataggaa aaagcaggca aaaaaatacc tccgatgctg ctctcatcgg aggtagaaaa
     4801 gccgtaatct gttttttagt gggcggccag ggcgctcttt ttgaccgtca ccttcatcgg
     4861 ctgctgcagg atgaaggtca actcgctgcc ggccgggtag aggaggttct ttttgccggt
     4921 caaaactgcg gcgccggtcc cggcggcggc acctgccact ccgccgataa tggcaccttt
     4981 ctttttgccg gcgatggcac caattgccgt tcccactgcg gctcccccgc cgatcatacc
     5041 gacgttgcgg ccggttttcg acttttcttt ggcttccacc gtggtggtgg tgacgtggta
     5101 ggcctggccg tgagcttcga tttcattcaa ggtcagcacc agttcggcgg ttgacttcag
     5161 tccggatgaa ccgcgcgcca ggataatttc gcccgtaatc ggcgcgccct cttcgatcac
     5221 ggtctggccg ttgacaacga tcggcttggc cagggtggcg tcaaacttct cgccggtctt
     5281 gttcttgccg gaattgatgg aatccgacag cgccaccact aactgcattc cggcggggac
     5341 ttccagcacc accggcttgg cttcttccgc cttgggcgcc tgctgtcccg ccgtgcggga
     5401 cgacttcttc atcgtcttgt tcgaggcttg cgtgttggtt tgcttttccg gcgctgcttg
     5461 cgtttcagcc ccctgatcgg cctgactcat ctggggcgtc tcttccggcg tttgctcggt
     5521 ctgtttctgg gaacagccag ccagaagcag tgccgcgcag gccagaagtg ttgcgaagta
     5581 tttcaccata ttttccttcc tcctttcgat gtcctatatg tcgtcacaaa tagtacgaat
     5641 gtttagggca aacgatagta cattgagcga gaagctgcaa gcaatttatg ttcagcaagc
     5701 ataagtcttg actcttgtag tgcagcgaaa ctttctaccc gacaatttct ttcaatcttt
     5761 cttactcttt tctagcttca ataccaaaaa gccgtgcacc gagtctaaac cagccgccag
     5821 taatggttgg tgagtaagga ttaggagaat agcgaagggt aaagtcccat tccagccacc
     5881 aaattgatat gttggtggca tggcaagacg actgaggtgt ccacgctgcg acagtggcaa
     5941 gtactggatt ctccgaggga gaaaacgccg gtgtgcacac
//
LOCUS       DBNGZD010000028         1761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_351409, whole genome shotgun sequence.
ACCESSION   DBNGZD010000028 DBNGZD010000000
VERSION     DBNGZD010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_351409"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..828
                     /locus_tag="AC16GM_00810"
     CDS             403..828
                     /locus_tag="AC16GM_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR2922250.1"
                     /translation="MTRRRRVEKGFTLIELMIVVVIIGILAALAIPRFMRAATKTKQS
                     EAKHILKQIYVQEHAYRQEKDFYIAYPPTGAGGTMPEIIIEIMPSARYTYSVAAGGSG
                     IVNSFVATATANLDDDATVDTWTIDNFGSLTNTINDVET"
     gene            1195..1623
                     /locus_tag="AC16GM_00815"
     CDS             1195..1623
                     /locus_tag="AC16GM_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR2922251.1"
                     /translation="MIRRDKKGQKGFTLIELMIVVVIIGILAALAIPRFMRASTKAKQ
                     SEAKQLLKQIYTMEQAYRQEKDFYQAYGATGANGVMAGIGVEIMASARYSYQIVAGGA
                     GIQNSFTATATANLDDDATIDTWTINEAGTLINTINDAET"
BASE COUNT          450 a          483 c          501 g          327 t
ORIGIN      
        1 gtcctacggg aattgccact tcctttatcg ccaccgctac cgtccccgct ccgggtttgg
       61 atgacgaccc ggctccggac atctggcaaa tcgacgacca gggggtcttg caaccggtca
      121 gcaacgacgc cgagcaataa gccgtcaccg cctttcgttc agaagcgttg ctccccgccg
      181 ttttctctga cccgtcacgc aactgtggca aattgcaaag gcagtagcag gaaaagtcac
      241 cagcagtgtg ggggaaaagc cagaccaacc tccggtaagc agaagtacaa ccgacggtta
      301 tccaaaaacg gcgggcacgg aacggcggtt gctctttcgg gaagtgacaa ggtgtaccct
      361 tgggtagact cagggcacac ataagcaaag tcgaggtgtg ctatgaccag aaggcgacga
      421 gtggaaaaag gttttaccct gatcgagttg atgatcgtgg tagtcatcat cgggattttg
      481 gcggcgttag ccatcccgcg cttcatgcgg gctgcgacga aaaccaagca gtccgaagcg
      541 aaacatattt tgaaacaaat ctacgttcag gagcacgcct accggcagga aaaggatttt
      601 tatattgcct atccccccac gggtgccggc gggaccatgc cggaaattat catagagatc
      661 atgccgtcgg cccgctacac ctactcggtg gccgccggcg gctcggggat cgtcaattcc
      721 ttcgtggcga ccgcaaccgc gaacttagat gacgacgcca cggtcgacac ctggaccatt
      781 gacaacttcg gttcgctgac caacaccatc aacgacgtag agacttagtt cttcccgaga
      841 gtagcacaga ccgaaaccac gaccagttca caccaacccc gccctccgta ccgggcgggg
      901 tttcggtttt gcgacaggta agtgggatca agtggtgaga cctgagcgat caggtacgga
      961 tcatccctct tccatctggc aaaatgcaag tcccagagcg aattcccgac gaaaggcacc
     1021 cgttcagaaa aaagacaact tgtgcagatt tttttcacca ccggtaacgt tcgattcaag
     1081 aagtagttac ggctaccgat aatcagggtg aattccggtg gttccggcac tgtggttgca
     1141 caactcacag cgggtaaaat ggtgaaaata gagcccctcg cgaaaggagg agcaatgatt
     1201 cggagggaca agaaaggcca gaagggtttc accctgatcg agttgatgat cgtagtggtc
     1261 atcatcggga ttctggcggc gttggccatc ccgcgcttca tgcgggcctc gacgaaagcc
     1321 aagcagtcgg aggccaagca gctcctgaag cagatttaca cgatggagca agcctaccgg
     1381 caggagaaag acttctacca ggcgtatggt gcgaccggtg cgaacggcgt catggccggg
     1441 atcggcgtgg agattatggc gagcgcccgc tacagctacc agatcgtggc gggtggtgcg
     1501 ggtattcaaa actcgtttac cgccaccgcg accgcgaact tagacgacga cgcgacgatt
     1561 gacacctgga ccatcaacga ggccggaacg ctgatcaaca ccatcaacga cgcggagaca
     1621 tgatctcatc ggcagttcgc acaaacccca tccgatatca agtaccgggc ggggtctcaa
     1681 ttgggtaaaa cggcgaaagc agaacccctc gcgaaaggag gagcaatgat tcggagggac
     1741 aagaaaggcc agaagggttt c
//
LOCUS       DBNGZD010000029        14141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1532425, whole genome shotgun sequence.
ACCESSION   DBNGZD010000029 DBNGZD010000000
VERSION     DBNGZD010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 14141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14141
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1532425"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1116)
                     /locus_tag="AC16GM_00820"
     CDS             complement(70..1116)
                     /locus_tag="AC16GM_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR2922252.1"
                     /translation="MRFGQPLILYLYILVAGLVVLLWWAGRQRRKLFSTWGDLSLLER
                     LTQTINLKSRRWKSVLLITGAGLLVFAAARPQYGQRLTYVKREGIDLLVAVDVSASML
                     AEDVKPNRLELARRDVAGLINRLQGDRIGLLAFAGVAFVQCPLTLDYGAAKLFLDVLN
                     PNLIPEPGTAIGEAIRTAQQAFVQKERKYKVLILITDGEDHDTDPIGAAKEAAREGIR
                     IYTIGMGSSDGEPIPLRGPNGQLIGYKKDESGQVVVSRLDETTLQKIALETNGKYYRG
                     TTGWLELDHIYDEIAKMEKKGLKGEYQTQYEDRYQYPLFLGLLLVLVEFILPERRKIQ
                     PEVSESVKRLRMSQ"
     gene            complement(1116..2105)
                     /locus_tag="AC16GM_00825"
     CDS             complement(1116..2105)
                     /locus_tag="AC16GM_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR2922253.1"
                     /translation="MRFASPWFLSLLVLLPAVIGYYLGRKRKEQPSLRYSYTGLFSGA
                     RASWRARLAWVSLALRSLMILLVVLALARPQKGRSSQEVESEGIDILLALDISSSMKA
                     EDFKPQNRLYVAKETIKDFIAGRKHDRLGLVVFARQAFTQCPLTLDYGVLLDFLHQVD
                     FGMVEDGTAIGMALITCVNRLKESQAKSKVIILLTDGVNNAGEVDPVTAAGAAQAFGI
                     RVYTIGVGQPGAALYPYDDPVFGRRYVYMPNEIDEASLAKIAELTGGRYFRAKTEKML
                     SEIYKKIDELEKTKIKVKEYRQYDERFWPFALGGLGFFLAEIFLANSLFRRMP"
     gene            complement(2107..3093)
                     /locus_tag="AC16GM_00830"
     CDS             complement(2107..3093)
                     /locus_tag="AC16GM_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR2922254.1"
                     /translation="MSGCKKILSGPLGSVVTALWLPVVACAVPADSIKPQVQVTTTVD
                     RKEITIGDRLKYTITVERDPKVKVAPFPLGGNLGAFEVKDFKPLPEKKMKGRVVQTTE
                     FLVTTFTTGEYVIPPIAVSYTDTTGKKQLLYSDSIKITVKSVGRKASDTDDIRDIKPP
                     LAVGSETLPFVVGGVLFLLLAAAGYLWWRKRRTVAVVGQLPPVDTRPVWEIAKERLLI
                     LKNSGLLSENRIKEYHFQLSEIVRWYLERRFGILALEQTTEEIRAALKKIDFPRRLFS
                     TAIGLLDFCDLAKFAKYQPPATEIEVNWQVAFQVVEETTPKPEPTAPTEVGT"
     gene            complement(3080..4003)
                     /locus_tag="AC16GM_00835"
     CDS             complement(3080..4003)
                     /locus_tag="AC16GM_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922255.1"
                     /translation="MTQERLLRGSWTFSLATLALVVVLFLLTVFKPSSSVNFEQEKRL
                     AAVLVENKLYRQAIDAYGKLLQSFPNDPKRVANLHYLIGSIFSDHLNAPEEGLAQFLM
                     ARALDPKSEFQKELSQRIVTSYERLGRTDEAKRELEKATALETKPVSAPEKEVVATIG
                     KRQITRKELERELQFVPPEVRQNFPDTKEGRRNFLNQYLGLELLYESAKRRGYADDSA
                     AITYGQRAQKEYATERVITEDIAARVAVTPDEVKKFYEQNKKKFGGKPFSAVADSVTS
                     LVRANKQKELYLQTIQNLWEKEKVQVFDERL"
     gene            complement(4031..4909)
                     /locus_tag="AC16GM_00840"
     CDS             complement(4031..4909)
                     /locus_tag="AC16GM_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR2922256.1"
                     /translation="MQLSEIIKHVRHIEIRSKRLVNELFSGEYHSIFKGRGMEFQEVR
                     EYQPGDDIRLIDWNVTARQGVPFVKKFREERELVVMLVVDHSRSGEFGTRQQFKNELA
                     AEICAVLAFAAIFNNDKVGMILFTDEVERYIPPKKGTSHVLRLVREALSFEPRRSGTS
                     LTTTQQFLNRVQKRKSVVFLISDFFDRDYQKALTISGRRHDLIAIRIIDPREQLVPPV
                     GLVELEDAETGLRVVVDTGDPNVLESFRRRAADHERRVAEAFKKSRTDRIAIQTGKSY
                     IEPLVAFFRQRERRFR"
     gene            complement(4917..5678)
                     /locus_tag="AC16GM_00845"
     CDS             complement(4917..5678)
                     /locus_tag="AC16GM_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922257.1"
                     /translation="MREPLCGVCEGIISKWETKFAPIFRQLASDNCPKISYEQWFAKF
                     VASVVWRVLLMFRQDGLLDHLLPKALVQVEPALEKWRTFMLGNTLNPGTFELHFVRFG
                     VIESVNFQIGAPNINRYLARAIDVDVVNAPVHSFVFAKLCHCMFFGFLGKPSRKLWQG
                     TRISPSRGTLGGRVSLVLPRGFDDYIIEKANRMFKDQKSLSPRQKEIIARSHRKNLDR
                     TTNSETFRVLQQDVAMFGRAAFVEANQTPPPESDQ"
     gene            complement(5862..6200)
                     /locus_tag="AC16GM_00850"
     CDS             complement(5862..6200)
                     /locus_tag="AC16GM_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922258.1"
                     /translation="MWLDCAKLERSLKTFPKESDGMYIVYDLFTFDNFFRLLLKEGLD
                     HDEALDFAVFNFSMSVLVWQERIHNKRYLRRLSAEQAIHPLEASRKARLIYDFMEIIR
                     RERRGPGKPK"
     gene            complement(6226..7212)
                     /locus_tag="AC16GM_00855"
     CDS             complement(6226..7212)
                     /locus_tag="AC16GM_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2922259.1"
                     /translation="MHQDIKAISELVEKESVAVRQLVAEIGKVIVGQKYMVERLLVGI
                     LANGHILVEGVPGLAKSLTVKTLADTIKAKFQRIQFTPDLLPADLIGTMIYQPGDGKF
                     TTKKGPIFANLILADEINRAPAKVQSALLEAMQERQVTIGEKTYPLDEPFLVMATQNP
                     IEQEGTYPLPEAQVDRFMLKLSVGYPNKEEELEILNRMTVGKSFNADAVVSPEEILRG
                     RKVIHQIYIDDKVKEYILNIVFATRQPETYGLKLKELIAYGASPRATIYLTLASKAHA
                     FLRGRGYVTPEDVKSIGPDVLRHRIIVTYEAEAEETTSDQLIRQIFDQIEVP"
     gene            complement(7295..8140)
                     /locus_tag="AC16GM_00860"
     CDS             complement(7295..8140)
                     /locus_tag="AC16GM_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HIR2922260.1"
                     /translation="MLARAKAFWEISRPVNVVITFASVGLGAYLTGSGVDWLSIAVAA
                     CTAGLICAGGNALNDLCDIGCDQINKPLRPLPSGRLSPKAVRSFVVLLFTSGVILAAS
                     FPAPVLGIAVGVVMLLVLYNFRLKRLPLVGNALVSLAAAASFPYGALAAKKEVVPEVW
                     FITLFAFLFHFGREILKDLEDLPGDLKVNARTFPIRYGLVPTRRLVAAVFFVLALSTL
                     IPALLKLFSWRYTVVVVGGVDLSLLYIVWSLARDASSLNLHRLSRLLKVDMVVSLLAV
                     FLGVQ"
     gene            complement(8144..9859)
                     /locus_tag="AC16GM_00865"
     CDS             complement(8144..9859)
                     /locus_tag="AC16GM_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2922261.1"
                     /translation="MVFRCARSVVGVFSTLFIFFGIFLSPCAAESEITFVGNHFFSTG
                     DLKKIAVQTPVDSLAARVSAAYRQEGFLTAQVNATSANGRITLEIAEGPRFRVSRVKL
                     RGAEPLPQAHVQRLVSSVAGRPASQTTFDRFIKQILELGDNTGFPYTQVWLDSVVINH
                     PPQADLQIRLLPGPQVVVREIRFPGLAAQEKKNLLRRLALREGDIFSQKSLERAAAEL
                     RQKRRVSVTGPPELYSGEQLADGIVVFPVKSRQTSSLDGALGYVPGAGSRKGNWVGGF
                     DLLVDEFGGARTFGIRWARKSLKTSEVNLAYTEENPWAWPLDFTFHLAQQQRDTLYFQ
                     LAVSGALNWEVLTRLTLGLKSGWEKVVPGDRAPLVVFPSRKFSLGGEVTYDGTDDLFN
                     PARGIRLSVASMVTRKRSFVLHGVAPEKPLLYQRSTTLDLTQYLPISRVTQDFFRLQG
                     AGIGSSERVVPLADQFFLGGQGSVRGYQKDQFSGSRVAWTNLEYRLRFGEGDRVFLFT
                     DAGYFYRREQSQSEIIFREATQVGFGLGLAVRRSFGEFEVSVGWPQHAAVGDGIVNFG
                     VVNRF"
     gene            complement(9859..12843)
                     /locus_tag="AC16GM_00870"
     CDS             complement(9859..12843)
                     /locus_tag="AC16GM_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922262.1"
                     /translation="MEESSIVSERPPRLPSAKTPAARPAPFDLSVSGAKSFAVELGPA
                     QAVGLAQGLNLSLSGQVSPNSRLAASISDRASPGAGFQTSRRLAELDQVKVEFSSPAL
                     QATVGDFRWQAGGSAGPGWAQTLSGFKGGFTGRGWKLQAVAAATKGRWQHKSFYGQPG
                     RQGPYLLGTGAVVPNSEKIYLNGEQLFSGSDRDYVIDYGSGSVTFTPRRPIGADVRIA
                     CDFETLEGDYPQHLYGGESEIQGLSGRARFSFDLLRAIDDRSSGFGASLTAAEEGRWG
                     QTGQGDGLVAKSGAQRVGFGQGDYVALDSSSGVKIYRYAGPGRGDWRVSFSVVGAGKG
                     DYLPLAGGGYQYVGYNQGTYAPIVFLPAPQGHWQISSGLAVTPLPSLSVSGQWRHTSL
                     EPDLTVDTKSTIPGNELRFGFAWGTGTAPVQVSGNFGLREKRFQALGDSTSQIREVSW
                     GIFGSTPEGDEHTGEFVATLNTPRVQGKLTTGLLAQSGNFAARRAGGSASLSLWRNSE
                     LHLKDEEIRGAQKNFSLSSVVPYLYRHASTAELVQRVGSWTVGAAWQQGTAWRAGGSY
                     SGFGGERFGVLSARVDRGRTAVAVSRRQSDVHQTVWQRFSEQILATLSSGWTRRAFSG
                     DLTYTRQLQSNHTSLPGRKSLDLALLRTAYRPSEGALAVDFFHSINQVGAGNYGERFE
                     SRELFPDTPGSSDSLTSRPYALYDSTIAGEPVPEFSQALRLEYHPRRNGSVVNARSVM
                     GDLAQRLSADVSLSQTHREKGYGYGDLGLLAFWRKSNPAALLFGEKRAAGEVKLWPVG
                     RQAHLFARGNLDRQQYRLFGSGLRERENHQYGMGIRGAFASKSTGEVEFYLTNKNEQS
                     VDQLTLGLSGRGGRTNVWYYLSRTLDLNGEIKILREREQVGGAQTVFYSAAPLVRLRP
                     LAGLSVSAQARWVAVTTQTPAAPLSFFQAEGRPVGQSWEWSVTGEWRLRTKLAGQWTV
                     SSRKDRWFGQTTAGRAELKAYF"
     gene            complement(13089..14099)
                     /locus_tag="AC16GM_00875"
     CDS             complement(13089..14099)
                     /locus_tag="AC16GM_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR2922263.1"
                     /translation="MFSGRPQPGWGRKARAALGVGCSVFLLVGCSASVSRKTEVLQTT
                     ATIEETPGLRLETIYVIRGQIGSAELKEPTAVVPDLFGNLFIVDSGQRRILKLDPQFR
                     LLAEFRVPGEGFSLFNGLGYAAATHAGEVFVGDTWARQVWHLDGLLRPLEPVEPRYAT
                     ADSVFQGPGGVAADESGLLTVADPANSVIFRIDVFGQKRVGEPFGHPGNLSSPAGLAL
                     DRWGNLFISDAGNRRIAVYDPGGRFIRDFGGRVLDTPAGIAVDPARRVWVADRRRNSI
                     VVFDPEGGVLTEQSDFGQLGGQLSKPTGLALDLRGKVYIADTDNNRLVICQIARETAP
                     KP"
BASE COUNT         3128 a         4177 c         3866 g         2970 t
ORIGIN      
        1 aaacccggcc tccgtacccc gatagggttg ccgacctgac ctccaggggg tacacccctt
       61 cgatcacttt cattgactca tcctcaaccg ttttacgctt tcggaaactt ccggttggat
      121 cttgcgccgc tccggaagga taaattcaac gagtaccaat agaagtccca ggaaaagcgg
      181 gtactggtag cggtcttcgt actgggtctg gtactcccct ttcaaacctt tcttctccat
      241 cttggctatt tcgtcataaa tgtggtccaa ttccagccag ccggtggtgc cgcggtagta
      301 cttgccgttg gtttcgagtg caattttctg caaggtcgtc tcgtctaaac ggcttaccac
      361 cacctggccg gattcgtctt tcttataccc aataagttgc ccgttcggtc cccggagggg
      421 gattggctct ccgtcggatg agcccatgcc aatggtgtat atccgaatcc cttcgcgagc
      481 cgcctctttg gccgcgccga ttggatcggt gtcgtggtcc tcgccgtcgg tgatgaggat
      541 cagcaccttg tacttccgtt ctttctggac gaaggcctgt tgagcggtac ggatggcttc
      601 gcctatggcc gtccccggtt cgggaatgag gttcgggttc aggacgtcca gaaacagctt
      661 ggccgcgccg taatccaagg tcaacggaca ctggacgaac gcgaccccgg caaaggccaa
      721 aagcccgatc cggtcaccct gcaggcggtt gattaatccg gctacgtcgc gccgggccag
      781 ttccaggcgg ttcggtttca catcttcggc cagcatcgag gcggagacat cgactgcgac
      841 taaaagatcg atgccctcgc gcttgacgta agtgagcctc tggccgtact ggggtcgcgc
      901 cgcggcaaag acgaggaggc cggccccagt tatcagaagc acgctcttcc agcggcggct
      961 cttcagattg atcgtctggg tcaggcgctc caggagcgaa agatcgcccc aggttgaaaa
     1021 gagctttcgt ctttgccgtc ccgcccacca gagcaaaacc accagcccgg cgaccagaat
     1081 gtacaagtag agaatcagag gttgtccgaa acgcactacg gcatcctccg gaaaagcgaa
     1141 ttggccaaaa atatttccgc caggaaaaac cccagcccgc ccaaagcgaa cggccagaag
     1201 cgctcgtcgt actggcggta ttctttgact ttgatcttcg tcttttctaa ttcgtctatt
     1261 ttcttgtaaa tctcagaaag cattttttcg gtcttagcgc ggaagtagcg cccgccagtc
     1321 aactccgcaa ttttcgcaag cgacgcttcg tcgatttcgt tcggcatgta gacgtagcgc
     1381 cggccgaaga ccggatcgtc gtacggatag agtgccgcgc ccggctgacc cactccgatg
     1441 gtgtaaacgc ggatgccgaa ggcctgggcc gcaccggcgg cggtcaccgg atcgacctcg
     1501 ccggcattat ttaccccgtc ggtcaagagg ataatcacct tgcttttcgc ctgcgactcc
     1561 ttgaggcggt tgacgcaggt gatgagcgcc atcccgatgg cagttccgtc ttcaaccatc
     1621 ccgaagtcta cctggtggag aaaatccagg agcacaccgt agtccaaggt gagcgggcac
     1681 tgggtgaagg cctggcgcgc gaagaccacg agccccaggc ggtcgtgttt acgcccggcg
     1741 atgaaatctt tgatcgtctc tttggcgacg tagagccgat tctgcggctt gaaatcttcg
     1801 gccttcatag aggaagaaat atccagggct aataggatat cgataccttc cgattccact
     1861 tcctgactgg agcggccctt ttgtggccgc gccaaagcca gaacgaccag aagaatcatc
     1921 agtgaacgca acgccaaaga cacccacgcc agccgcgccc gccaagaagc cctggccccg
     1981 gagaacaaac ccgtgtaaga atagcgcagg gacggctgtt cttttctttt ccgccccagg
     2041 taatacccaa tcactgccgg gagaagcacc aagaggctca aaaaccaggg tgacgcaaaa
     2101 cgcatttcag gtgcctacct ctgttggagc ggttggttcc ggcttcggcg ttgtttcctc
     2161 aaccacctgg aaagccacct gccagttcac ttcaatttca gtggccggcg gctggtattt
     2221 ggcgaacttc gccaggtcgc agaaatcaag cagcccgatg gcggttgaga aaagccgccg
     2281 gggaaaatcg atctttttga gcgccgcgcg gatttcttcg gtggtctgct ccagagcgag
     2341 aatgccgaaa cgccgctcga ggtaccagcg gacgatttct gacagttgga agtggtattc
     2401 tttgatccgg ttttcggaca acaaacctga attcttcaaa atcaagagtc tctctttggc
     2461 aatttcccag actggccggg tgtcaaccgg cggcagctga cccacaaccg caaccgtccg
     2521 gcgtttccgc caccagaggt agcccgccgc tgcgagaagc aaaaagagca ccccgccgac
     2581 cacgaagggc agcgtttccg agccgacagc cagcggcggc ttgatgtccc ggatgtcatc
     2641 ggtatccgaa gctttccgcc ccacactttt aacggtgatt ttgatggaat ccgaatagag
     2701 cagctgcttt tttccggtcg tgtcggtgta cgacacagcg atcggcggaa tgacatattc
     2761 tccggtagtg aaggtggtga ccaggaactc cgttgtctgc accacacgtc ccttcatctt
     2821 tttctccggg agcggcttga aatccttgac ttcgaaggcc cccagattgc ctcccagcgg
     2881 aaacggagcg acctttactt tcggatcgcg ttccacggtg atcgtgtact tgaggcggtc
     2941 accgatggtg atttccttgc ggtcaacggt cgtggtaacc tgcacctgtg gttttatgct
     3001 atccgctggc acggcgcatg ccaccaccgg tagccaaaga gcggtaacga ctgatcccag
     3061 tggtcctgag aggattttct tacaaccgct catcgaagac ctgtaccttc tctttctccc
     3121 agaggttctg gatcgtctgc aggtagagtt ctttctgctt gtttgcccga actaacgaag
     3181 taaccgaatc cgccaccgca gaaaaaggtt tgccaccgaa ctttttctta ttctgctcgt
     3241 agaacttttt cacctcgtcc ggtgtcacgg caaccctggc ggctatgtcc tcggtaatca
     3301 cccgttccgt ggcgtattcc ttttgggcgc gctggccgta agtgatagcg gcggaatcgt
     3361 cagcgtaccc ccgccttttg gccgactcat agagcaattc caaaccaagg tactgattca
     3421 gaaaattgcg ccgtccttcc ttggtgtctg ggaagttttg tcttacctcc ggcggcacga
     3481 attgcaattc ccgttcgagt tcttttctgg taatctgccg cttcccgata gtggccacca
     3541 cttccttttc cggcgcagaa accggtttgg tttccagcgc ggtggccttc tctaactccc
     3601 ttttggcttc gtcggttcgc cccaaccgct cgtagctggt cacgattctc tgcgagagct
     3661 ctttttgaaa ctccgatttc ggatcgagcg cccgggccat caggaactgc gccagccctt
     3721 cttccggcgc gttcagatgg tcggagaaaa ttgagccgat gaggtagtgg aggttggcga
     3781 cgcgtttcgg gtcgttggga aacgactgca acagcttccc gtaagcatcg atggcctgcc
     3841 ggtagagttt attctccacc agcaccgcgg ccaatcgctt ctcctgctcg aaattgaccg
     3901 aactcgacgg cttgaagacc gtcagcagaa aaagcacgac gaccagtgcc aacgtcgcta
     3961 aagaaaaggt ccagctgcca cgtaacaggc gctcctgcgt cacgttttct cctctgtgtc
     4021 tatctacgta ctaccggaac cggcgttccc gctgccggaa aaaggccacc aacggttcga
     4081 tgtacgattt gccggtctgg atagcaatcc ggtcggtccg gcttttcttg aacgcctcgg
     4141 ccacgcgccg ttcatggtcc gccgcccggc gccggaagct ctccagaaca ttcggatcac
     4201 cggtgtccac caccacccgc agccccgttt ctgcatcttc taattccacc aggccgaccg
     4261 ggggcaccag ttgttcccgc gggtcgatga tgcggatggc aatcaagtcg tgcctgcggc
     4321 cggagatggt cagggctttc tggtagtccc ggtcgaagaa atcagaaatc aaaaagacga
     4381 cgctcttgcg cttctgcacc cggttcagaa attgctgcgt cgtagtcaga gacgtccccg
     4441 agcgccgggg ttcaaaggag agcgcctcgc gcaccaaccg gaggacgtgg ctggtccctt
     4501 ttttgggcgg gatgtagcgc tccacctcgt cggtgaagag gatcatcccc accttgtcgt
     4561 tgttgaaaat cgcggcgaaa gccaggacgg cgcagatttc ggcggccagt tcgttcttga
     4621 actgctgccg ggtgccgaat tcgccggagc ggctgtggtc caccaccagc atgaccacta
     4681 attcccgctc ctcccggaac tttttgacga acggcacccc ctgccgggcg gtgacgttcc
     4741 agtcgattag ccggatatcg tcgccgggct ggtactcccg cacctcttgg aactccatac
     4801 cccggccctt gaagatggag tgatactccc cggaaaataa ttcgttcacc agccgtttgg
     4861 agcggatctc gatgtgccgg acgtgtttga tgatctcgga gagttgcata ggtgaactat
     4921 tggtctgatt cgggaggagg agtttggtta gcttcaacaa aggctgctct gccaaacatg
     4981 gccacatctt gttggagtac ccgaaaagtc tctgaattcg ttgtgcggtc aagatttttt
     5041 ctatggctcc gggcaatgat ctctttttgg cgcggagaca gagatttttg atctttaaac
     5101 attcggtttg ctttctctat gatgtagtca tcaaaacccc tcggtagaac caacgaaact
     5161 cgacctccta aagtgccccg gctgggacta attcgtgtac cttgccagag cttacggctt
     5221 ggtttcccaa ggaaaccgaa gaacatgcag tgacataatt tagcgaatac aaaggagtgc
     5281 actggtgcat tcacaacatc gacatcaatt gccctggcta aatacctatt gatattggga
     5341 gcaccgattt gaaagtttac tgattcaatt actccgaaac gcacaaaatg caattcaaag
     5401 gtacccggat ttagtgtatt tccgagcatg aaagttcgcc atttctccaa agctggttcc
     5461 acctgcacca gtgctttagg aagaagatga tccaatagac catcctggcg gaacattaaa
     5521 aggacacgcc aaacgacgga ggctacgaac ttggcaaacc attgttcata tgagattttt
     5581 ggacagttgt cgctcgcaag ttgacgaaag ataggagcaa atttcgtttc ccactttgaa
     5641 ataatacctt cgcaaactcc acataatggc tctcgcatgt gaggcaaatc ctgtacccgt
     5701 ctatttacgt tcttactaaa ccggatgtag ccggtagaag aagtttcctt catccatttt
     5761 attacaaaac tggggattag gtggctttcc ttaagtcggg atggcttttg gcacaatgaa
     5821 cagactgtcg ggttcatatt atcgagaaaa gggttgcctt cttacttcgg tttaccgggt
     5881 cctcttcttt ccctccgtat aatttccata aagtcataga tcaaccgggc cttacgagat
     5941 gcttccaggg gatgaatggc ttgctctgcg gaaagccgcc gcaggtagcg tttattatga
     6001 attcgctcct gccataccaa aacactcata gaaaagttga atacggcaaa gtctagagct
     6061 tcgtcatggt ctaagccttc tttcagaagc aggcggaaga aattatcaaa agtaaaaagg
     6121 tcataaacga tatacatgcc atcactttct tttgggaagg ttttgagact acgctccagt
     6181 ttagcacaat cgagccacat aagggcgact ctcgtatgtt cgtatctacg gcacctcgat
     6241 ctggtcgaaa atctgccgga tgagctggtc ggaggtggtc tcctcggctt cggcttcgta
     6301 ggtgacgatg atccggtggc ggagcacgtc tggcccgatc gatttcacgt cttcgggcgt
     6361 cacgtagccg cggccccgca aaaaggcgtg ggctttggag gccagtgtca ggtaaatggt
     6421 ggcccggggc gaggcgccgt aggcgatcaa ctctttcagc ttcaacccgt atgtttccgg
     6481 ctggcgggtg gcaaagacga tgttcaaaat gtactctttg accttgtcgt cgatgtagat
     6541 ctggtgaatt accttccgcc cccgcaggat ttcttcgggt gacaccacgg cgtcggcatt
     6601 gaaggatttc ccgacggtca tccggttcaa gatttccaat tcctcttcct tattcgggta
     6661 gcccaccgag agtttgagca taaagcggtc tacctgcgct tccgggaggg gataagtacc
     6721 ttcctgctca atcgggttct gggtcgccat caccaaaaac ggttcatcaa gcgggtaggt
     6781 cttctcgcca atggtcacct gccgctcctg catcgcttcc aaaagggccg actgcacctt
     6841 ggccggcgcg cggttgattt cgtcggctaa aatcaggttg gcaaagatgg ggcctttctt
     6901 ggtggtgaat ttgccgtcgc cgggctgata gatcatcgtg cctatgaggt cggcgggcaa
     6961 aaggtcgggg gtgaattgaa tcctctggaa cttggccttg atggtatccg ctaaagtctt
     7021 caccgtgaga cttttggcca gcccgggtac cccttcgacc aaaatatgcc cgttggctaa
     7081 aatgccgact aagagccgct ccaccatgta cttctgcccg acgatcacct tgccgatttc
     7141 ggccaccaac tgccggaccg ccaccgattc cttttcgact aattcactaa tcgctttgat
     7201 gtcctggtgc atccgacctc cagttcttca atctctatta ttcggagata aaaccaatat
     7261 gttggcaatt ttcgagaggg tcaagtggag aaggttactg tacacccaga aagaccgcca
     7321 gaagactcac caccatatcg accttgagca gtcgggacaa ccggtgcagg ttgagactgg
     7381 aggcatcccg ggccagcgac cagacaatgt acaggaggga aaggtccact ccgccgacga
     7441 cgactacggt gtaacgccag gaaaaaagtt tgagcagtgc cggaatgaga gtagacaacg
     7501 ccagaacgaa aaagactgct gccaccagcc gccgggtcgg caccaaaccg tagcggatgg
     7561 gaaaggtgcg ggcgttcact ttcagatcgc ccggcaggtc ttcgaggtcc ttcaaaattt
     7621 cccgcccaaa gtgaaagaga aacgcaaaaa gagtgatgaa ccaaacttcg ggaacgacct
     7681 cttttttggc cgccagcgcc ccgtagggaa aactggccgc tgcggccagg ctgaccaagg
     7741 cgttgcccac caagggcagc cgcttgagcc ggaagttgta aagaaccaag agcatgacga
     7801 cgccaacggc aattcccaac accggagccg gaaaagaagc cgccaagatt actccggatg
     7861 tgaagaggag caccacgaac gagcggacgg ctttcgggga caacctcccc gaaggtaacg
     7921 gccggagcgg tttattgatt tggtcgcagc cgatgtcgca caggtcattg agggcattgc
     7981 cgccggcgca gattaatccg gcagtgcacg cggctaccgc aatggaaagc cagtccaccc
     8041 cgcttcccgt cagataggca ccgagaccga cggatgcaaa ggtgatgacg acgttcaccg
     8101 gacggctgat ttcccaaaac gccttagccc gtgccagcat tgtctaaaac cggttaacga
     8161 cgccgaagtt cactatccca tcgcccacag ccgcgtgctg gggccacccg actgagacct
     8221 cgaactctcc gaacgaacgc ctcaccgcca gacccaaacc aaagccgacc tgcgtcgctt
     8281 cccggaaaat aatctcactt tgtgattgtt cacgtcggta aaaatatccg gcatcggtaa
     8341 aaagaaaaac ccggtctccc tcaccaaacc gaagccggta ttccagattg gtccaggcca
     8401 cccggctgcc cgaaaactga tctttttggt acccccgcac actcccctgg ccgcctaaga
     8461 aaaactgatc cgcaagcgga actactctct cactggaacc gatgccggca ccttgcaatc
     8521 ggaaaaaatc ttgcgtcact ctggagattg gtaggtactg cgtcagatcc agagttgtcg
     8581 aacgctggta aaggaggggt ttttctggtg caacgccgtg caacacaaaa ctccgcttgc
     8641 gagtgaccat acttgcaacg gaaagccgga tgccgcgcgc ggggttgaac agatcatctg
     8701 tgccgtcata cgtcacttca cctcccagag aaaatttgcg gctgggaaag acgacaagcg
     8761 gggctctgtc tccgggaacc accttctccc atccgctttt caaccccagc gtcagacggg
     8821 tcagcacttc ccagttgagt gcacccgaga ccgccagttg aaagtacagc gtatcgcgct
     8881 gctgttgggc gagatgaaag gtaaaatcga gcggccaggc ccaggggttc tcttcggtgt
     8941 aagccaggtt gacttccgag gttttaagac ttttccgggc ccagcgtatt ccgaaggtac
     9001 gcgccccgcc gaattcatca accaacaggt cgaatccgcc gacccaattc cccttgcgcg
     9061 atcccgcgcc gggcacgtag cctaaggcgc catccaggga actggtctgg cggcttttga
     9121 cagggaaaac tactatgccg tcggccagct gctcgcccga atagagttcc ggagggccgg
     9181 taacgctgac cctgcgcttt tggcgcagct cggccgcggc gcgctccagt gatttctggc
     9241 tgaatatatc cccctcgcgc aatgccagac gccgcagcag attttttttc tcttgcgcag
     9301 cgagtccggg aaaacgaatt tcacgtacca ctacctgcgg gccagggaga agccgtattt
     9361 gtaagtcggc ctgcggcgga tgattgatca ccacactatc cagccagact tgtgtataag
     9421 ggaaacccgt attatcaccc aactcaagaa tctgttttat gaaacgatca aaggtcgtct
     9481 gggaagccgg gcggccggca acagaagaga caagccgctg cacgtgcgcc tgcggcagtg
     9541 gttccgcacc acgcagtttc acccggctca cccgaaaacg cggcccctcg gcaatttcca
     9601 gagttatcct gccgttggct gacgtggcgt tgacctgggc ggtcaaaaag ccctcctgac
     9661 ggtaggcggc gctcacccgc gcggctagcg aatcgactgg cgtctgaacg gctatttttt
     9721 tcagatctcc ggtagagaaa aagtgattgc cgacaaatgt gatctccgac tctgcggcgc
     9781 aaggcgaaag aaaaatgcca aagaaaatga acaatgtgga aaaaacaccg accacactcc
     9841 gggcgcagcg aaaaaccatc agaagtaggc tttcaattcc gcccgccccg cggtggtctg
     9901 gccgaaccac cggtctttcc ggctggaaac ggtccactgc ccggccagtt tggtgcgcag
     9961 ccgccactca ccggtcaccg accactccca gctttgaccg accggccgcc cttcggcctg
    10021 aaagaacgag agcggagcag ctggcgtttg cgtcgtcacc gccacccagc gggcttgcgc
    10081 ggaaaccgaa aggccggcca gcggccgtaa gcgcaccaac ggcgccgccg agtaaaacac
    10141 cgtctgcgcc ccgccgacct gttcccgctc gcgtaaaatt ttgatttcgc cgttgagatc
    10201 gagggtacgt gagaggtagt accagacatt cgtacggccg ccccgccccg atagtcccag
    10261 ggtcagttga tcgactgatt gttcattttt gtttgtgagg tagaactcca cttcgccggt
    10321 ggacttggac gcaaaggcgc cgcgaatgcc cataccgtat tgatggttct ctcgttcccg
    10381 cagcccggac ccaaagagcc ggtactgctg ccggtcaaga ttaccacggg caaacaggtg
    10441 cgcctgccgg cccaccggcc agagtttcac ctccccggct gcgcgctttt ccccgaaaag
    10501 gagtgcggcc gggttgctct tgcgccagaa ggccaaaaga cccaaatccc cgtacccata
    10561 gcccttttcc cggtgcgtct gagataggga aacatcagcc gacagccgct gcgcgaggtc
    10621 ccccatgaca ctgcgtgcgt tcacgaccga cccattccgg cggggatggt actccaaccg
    10681 caacgcctgt gagaattcgg gcaccggctc ccctgctatg gtcgaatcgt agagcgcata
    10741 gggccgcgat gtcagtgaat cggaacttcc cggcgtgtca ggaaataact ctcttgattc
    10801 gaaacgctca ccgtagttcc ctgcaccgac ttgattgatc gagtggaaga agtcaaccgc
    10861 cagcgcaccc tcagacggcc ggtaagccgt ccgtagcagg gccaggtcaa gcgactttct
    10921 gccggggagg ctggtgtgat tggattgcaa ttgacgtgtg taggtcagat caccggaaaa
    10981 ggcacgtcgc gtccagccgg aggaaagcgt ggccaggatt tgctccgaaa acctttgcca
    11041 gacagtttga tggacgtcac tttgccgtcg ggaaaccgcc acagccgtcc ggccgcggtc
    11101 cacccgcgcg gatagcactc caaagcgctc cccgccgaaa ccggagtagg aaccgccggc
    11161 ccgccaggcc gtgccttgct gccaggcagc accgacggtc cacgacccca cccgctgcac
    11221 cagttccgcg gtgctcgcgt gacggtaaag gtatggaacc acagacgata aactgaaatt
    11281 cttttgcgca ccgcgaatct cttcgtcttt cagatgcagt tcgctattac gccaaagtga
    11341 gagggaggcg gacccgcccg cccggcgcgc agcaaaattg ccggactgag cgaggagacc
    11401 ggtggtcagc tttccctgta ctctcggcgt gtttagggta gccacaaatt cgcccgtatg
    11461 ctcatcgccc tccggtgtac tgccgaagat accccaactc acttcccgga tctgactcgt
    11521 tgagtcgccg agtgcctgaa accgcttctc ccgtagtcca aaatttccgc tcacctgcac
    11581 cggtgcggtg ccggtacccc aagcaaaacc gaaacgcaat tcgtttcccg gaatcgtgct
    11641 tttcgtgtcg acggtcagat ccggctcaag gctggtgtgc cgccactgcc cggagaccga
    11701 gagggatgga agtggtgtga ccgcaagacc ggaactgatc tgccagtgcc cttgcggcgc
    11761 aggcaggaaa acgatcggcg cgtaggttcc ctgattgtag ccgacgtact ggtagccgcc
    11821 cccggccaga ggcaggtagt cacccttgcc ggcaccgacc accgagaaac tgactctcca
    11881 gtcgccgcgc cccgggcctg cataacggta aatcttaacc ccggatgaac tatctaaagc
    11941 aacgtagtca ccctgcccaa agccgacgcg ctgggcaccg cttttggcaa ccagaccatc
    12001 cccctgaccc gtttgacccc agcgcccctc ttcggccgca gtcaaactcg caccaaaacc
    12061 ggaacttcgg tcatcaattg cgcgcaagag atcaaacgaa aaccgtgctc gaccactcaa
    12121 tccctgaatt tcactttcac cgccgtagag gtgttgggga taatctcctt ccagagtttc
    12181 gaaatcgcag gcgatgcgga catcagctcc aatcggccgc cgcggcgtga aggtgactga
    12241 accggaacca tagtcaatga catagtcgcg gtccgaaccg gagaacaact gctcgccatt
    12301 gaggtagatt ttttcggagt tcggcaccac cgcgcccgtg cccagaaggt acgggccctg
    12361 cctccccggc tggccgtaga aactcttgtg ctgccaccgg cctttggtcg ccgccgccac
    12421 cgcctgcagt ttccatccgc gtccggtaaa tccgccctta aatcccgaaa gcgtctgagc
    12481 ccagcccggc cctgccgatc cgcccgcctg ccagcgaaaa tcgcccaccg tcgcctggag
    12541 ggcaggcgaa ctgaattcga ctttgacctg atccaattca gccagccgcc gcgaggtctg
    12601 aaaaccggcg ccggggctgg cgcgatcaga aattgaggca gccaggcggc tgttgggact
    12661 gacctgcccc gaaagcgaga ggtttaaacc ctgcgccaga cccaccgctt gcgccggccc
    12721 caattctacc gcaaacgact tggctcccga aaccgagagg tcaaaaggag cgggccgagc
    12781 cgctggtgtc tttgcggagg gtagacgggg tggtcgttcg gaaacgattg aggattcttc
    12841 cacgaacgtt cggggttggc ggttgaaata gcgttggccg atgggcagaa cccgaaggga
    12901 aagattgaga gactgtgcgg tggaactcgg aagttctgaa gttgcagcgg gaagagtgtc
    12961 gggaagagaa taatggagat tcccgtacgc attctccgga gggatataga gcgccgaaaa
    13021 acagggggac gtgctgaggg aaaaaacagc actacaaaaa aagaggtgcc gggataaaga
    13081 acacggtttc acggtttcgg tgcagtctcc ctggcaatct gacagatcac caggcgatta
    13141 ttatcggtgt cggcgatgta gactttcccc cgcagatcca gcgccaggcc ggtcggctta
    13201 gagagctgcc ctcccagctg gccgaagtcg ctttgttccg tcaaaacacc gccttccggg
    13261 tcaaacacca cgatgctgtt tctcctccgg tcggccaccc agacccgccg ggccggatca
    13321 acggcaatgc cggcgggtgt atccagcacc cggccgccga agtcacggat gaagcgtccg
    13381 cccggatcgt aaacggcaat ccgccggttg ccggcatcgc tgatgaagag atttccccag
    13441 cggtcgagcg ccagtcccgc aggggaagag agattccccg gatggccgaa cggttccccc
    13501 acccgcttct ggccaaagac gtcaatccgg aaaatcacgc tattggccgg gtcggccacg
    13561 gtcaaaagac cgctttcgtc tgcggccaca ccgcccggcc cttgaaaaac ggaatcggca
    13621 gtcgcgtacc ggggttcgac cggctccagc ggccgcagca acccatccag atgccagacc
    13681 tggcgcgccc aggtgtcgcc gacaaaaacc tcaccggcgt gcgtggcggc cgcatacccc
    13741 agaccgttga aaagtgaaaa cccttcgccg ggcacccgga actcggccaa aaggcgaaat
    13801 tgcggatcaa gtttcaggat acgcctttgc cccgaatcga cgatgaagag attgccgaac
    13861 aggtcgggga cgacggctgt cggctctttc agttcggcgg agccaatctg gccgcggatg
    13921 acgtagatgg tttccagccg gagacctgga gtttcttcta tggtcgcggt ggtctggaga
    13981 acctcggttt tccgggaaac cgaggccgaa cagccgacca aaaggaaaac cgaacacccc
    14041 acacccagcg cggctctggc cttgcgcccc caccccggtt gtgggcgtcc ggaaaacata
    14101 gacagtaatt agaaggcttt cgtgacggtc agtcgaaaac c
//
LOCUS       DBNGZD010000030         5441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1046205, whole genome shotgun sequence.
ACCESSION   DBNGZD010000030 DBNGZD010000000
VERSION     DBNGZD010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1046205"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="AC16GM_00880"
     CDS             complement(<1..996)
                     /locus_tag="AC16GM_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2922264.1"
                     /translation="MIEKIIAYSAKNRFIIILLFILVVSWGIWALFRTPLDAIPDLSD
                     NQVIVFTEWPGRSPKVIEDQITYPLASNLQGLPHLKVVRASSAFGFSMIYVIFEDNVD
                     IYWARTRVLERLNYAQNLLPPGVVPTLGPDGTGVGHVFWYTVEGDGYDLGTLRALQDW
                     YIRYQLNSVPGVAEVASIGGFVKQYQVDLDPQKLLAYHVPVKEVLNAIKMSNQDVGGR
                     LVEATDMEYVVRGLGYIQSTTDIENVVVGATKEGVPIYVRNIGTVQLGGDIRRGVLDK
                     NGVGEVVGGIIVMRYQENAHEVIKRVKAKIKEIAPGLPPGVKIVPFYDRYTLIHNA"
     gene            complement(1203..2477)
                     /locus_tag="AC16GM_00885"
     CDS             complement(1203..2477)
                     /locus_tag="AC16GM_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2922265.1"
                     /translation="MKRIIIGIAVLAAAVLATFYLTRSAGRKAVRQVTAQAEEKEIYV
                     CPMHPMVTSDKPGNCSICGMDLVKRSSSEELTLTAQEELVAHEVRLSPTQQVLANVAT
                     EAVAVRLLAKEITTVGTIDYVEGNIANLSARVAGRTDSLFVNYTGERVTRGQPLLSMY
                     SPDIVGAEQEYLLALDSNNPALLESARRKLILWGITAEQIDRLGQSREMRDHLTIFSP
                     ISGTVIDKLVHLDMYVAAGQPLYNIADLSTVWMWAEIYEYEFANVRVGQSVEVTAVAY
                     PGEKFYGTINFVSPVVDTLTRTVRIRAEFDNPSDRLKPGMYVDARIKVNLGRKLAIPA
                     SAVLNTGQRQVAWVEKEPGVYTPRDVQIGQRAGNYCEVLSGVAAGEKVVTQAGFLIDS
                     ESQLARLSGAHAGHDTTSGKKQKEAMPGMPGM"
     gene            complement(2506..3813)
                     /locus_tag="AC16GM_00890"
     CDS             complement(2506..3813)
                     /locus_tag="AC16GM_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2922266.1"
                     /translation="MRRIRSGLKGSFLLFSCLAIGFFPGVSPAQDTTRTPLAQLVAEA
                     VRVNPNLRVAARNIEKAQAEVARVGYLPDPELMLGVENLPTSFSFREDEMTMKSIGIS
                     QMFPFFGKLGTKKNVVRQAVEVARWNYEEQKLELIRQVKETYFELFAQRRSQELTQKA
                     ITILSDFVALTRTRYEVGTGLQQDLLMATVEQARLEEKLANLEKRTVAVQNELKSLLD
                     RGPETPDIIPEEVKPVLYTPNRDSLLAGLTETRPMLKMLRADILKAQAEYKAAKREAW
                     PDFTWSVAYGIREGLRTPGMPGQDLDNMVTARLGLNLPINSSRRQGKEAAQASLELAN
                     AVSLLTHHAKHDVPLRIDDYLTEIDRNGRLILLYEKTILPQGQLAVQAAQVNYQVGKI
                     DVLTWLSNQLNLLDYQIDYFMTVSEYQKSIARLEAETGVTLQK"
     gene            complement(3955..4308)
                     /locus_tag="AC16GM_00895"
     CDS             complement(3955..4308)
                     /locus_tag="AC16GM_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922267.1"
                     /translation="MTGLAIIFFAVLFVQITSSPVQAKDQMPCCQGHSQTGQAAAQEQ
                     SHARPCPMHPGEKSTHPCKCLLGCSCQGTTVAATVPLPETKLLLDRLVSPVVNSQPIS
                     RSSEPSPPPPKTLLS"
     gene            complement(4506..5000)
                     /locus_tag="AC16GM_00900"
     CDS             complement(4506..5000)
                     /locus_tag="AC16GM_00900"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR2922268.1"
                     /translation="MAPVTTGKKAPTFQLKGIDGKPYEFKDDGTSALTVLSFFKNSCP
                     TCQLAVPYLERLYQAYQQKGVRFWGIEQDPADQSKEFARQYGLSFPVLPETAPYPVSN
                     AYGISIVPTIFLIDGTGQVLSSTVAFVKQELKNLSQEIAKRVNIAPAEIFASGDTAPA
                     LKPG"
     gene            complement(5061..5222)
                     /locus_tag="AC16GM_00905"
     CDS             complement(5061..5222)
                     /locus_tag="AC16GM_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922269.1"
                     /translation="MERGKTAEQVVVTFRPDTISGQALADKIADVGVPGFFRRQVRRL
                     GHFLHRETK"
BASE COUNT         1076 a         1606 c         1508 g         1251 t
ORIGIN      
        1 tgcgttgtgg atcaaagtgt agcgatcgta gaagggcacg attttcacgc cgggcgggag
       61 gccgggggca atctctttga ttttcgcctt gacccgcttg atcacttcgt gggcattctc
      121 ctggtagcgc atgacgatga tgccgcccac cacttcgccc accccgtttt tgtccaaaac
      181 gccgcggcgg atgtcgccgc ccaactgcac cgtgccgatg ttgcgcacgt agatcggcac
      241 gccttctttc gtggcaccca ccacgacgtt ttcaatgtcc gtggtgctct gaatgtaccc
      301 caggccccgc accacgtact ccatgtcggt ggcctccacc agccgccccc ccacatcctg
      361 gttggacatc ttgatggcgt tcaggacttc tttcaccggc acgtggtagg ccagaagttt
      421 ctgcgggtct aaatcgacct ggtactgttt gacgaatcct ccgatggagg ctacttcggc
      481 cacccctgga acagaattca attgatagcg gatgtaccag tcctggagtg cccgcagcgt
      541 ccccaaatcg tacccatcac cctccaccgt ataccagaag acgtggccca cgccggtgcc
      601 gtccggaccc agagtcggca ccacgccggg cgggagaagg ttctgcgcat agttgagccg
      661 ctccaggacc cgggtacggg cccagtagat atcgacgttg tcttcgaaga tgacgtaaat
      721 catcgaaaac ccgaaggccg aactggcgcg cactaccttc aggtgtggca gtccctgcag
      781 gttcgaggcc agagggtagg tgatctggtc ttcgatgacc ttggggctgc ggcccggcca
      841 ttcggtgaaa acgatcactt gattatcgga taagtcgggg atggcgtcca ggggggtgcg
      901 gaagagcgcc cagatccccc agctgacgac gaggatgaag agtaagatga tgatgaaacg
      961 gtttttggcc gaataggcga tgattttctc tatcatggct tagccctttt gtgaatctcg
     1021 tctgcgggga gggtcgagtt cactcgaccg ttgtgaccga acgcgactga agtcgcgtct
     1081 cccaagtcaa ctgactctct ctgtagcccc gctctccaga ccagggctac aggccccgca
     1141 agatcaatgg aatctgacgt tatgcctcgt gagaaagaac ccatttttta attctcctcc
     1201 ggttacatcc ccggcatgcc cggcattgct tctttttgtt tctttcctga cgtcgtgtcg
     1261 tggccggcgt gcgccccgct caggcgcgcc aactggctct ccgaatcgat gaggaagccg
     1321 gcctgggtca ccactttttc gccggcggcg acgcccgaca gaacttcgca gtaattccct
     1381 gcccgctggc cgatctgcac gtcacggggg gtatagacgc ccggttcttt ttccacccag
     1441 gccacctgcc gctgccccgt attgagcacg gcactggccg gaatggcgag tttccgaccc
     1501 aggttgactt tgatgcgggc atccacgtac atgcccggtt tgagccggtc gctggggtta
     1561 tcgaactctg ctctgattct caccgtgcgg gtcagcgtgt ccaccaccgg cgacacgaag
     1621 ttgatcgtgc cgtaaaactt ctcccccggg taggccacgg cggtcacttc caccgactgg
     1681 ccgacacgga cgttggcgaa ttcgtattcg tatatctcgg cccacatcca gaccgtggag
     1741 aggtcggcga tgttgtagag cggctggccc gcggcgacgt acatatcgag gtgaaccagt
     1801 ttgtcgatca ccgttccgga gatgggcgag aaaatcgtca ggtggtctct catctcccgc
     1861 gactgcccca ggcgatcaat ctgttccgcg gtgatccccc agagtatgag tttccgccgg
     1921 gccgattcca aaagcgccgg attgttacta tccagggcca ggaggtactc ctgctcagca
     1981 cctacgatgt cgggactgta catcgagagg agcggctggc cccgcgtgac ccgctcgccg
     2041 gtataattga cgaagaggga atcagtccgt cccgccacgc gcgcggagag gttggcgata
     2101 ttcccctcca cgtagtcgat cgtgcccacg gtggtgatct ctttggccag cagccgcacc
     2161 gccacggcct cggtggccac gttggccaag acctgctgtg tgggcgagag ccgcacttcg
     2221 tgggcgacca gttcttcctg ggccgtcagg gtgagttctt ccgaactact cctcttcact
     2281 aagtccatcc cgcagatgga gcagttcccc ggcttgtccg aagtcaccat cgggtgcatc
     2341 gggcaaacgt aaatttcctt ttcttccgct tgcgccgtga cctgcctcac cgcttttcta
     2401 ccggcacttc tggtgaggta aaacgttgcc agtacagcgg ccgccagcac cgcaatccct
     2461 atgatgatgc gcttcatctt ctcctccatt tcatttcatc acccgtcact tttgtagcgt
     2521 tactcccgtc tcggcctcca gacgggcgat gctcttctgg tactcagaca ccgtcataaa
     2581 gtagtcaatc tggtagtcca aaaggttcag ttgattcgaa agccaggtca aaacgtcgat
     2641 tttccccacc tggtagttca cttgggccgc ctgcacggca agttgcccct gcgggagaat
     2701 cgtcttttca tagagcagga taagcctccc gttccggtct atttcggtga ggtagtcgtc
     2761 aattctgagc ggcacgtcgt gcttggcgtg gtgggtcaaa agcgaaaccg cgttcgccag
     2821 ctccagactg gcctgggcgg cctcttttcc ctgacgccgg ctggagttta tgggcaggtt
     2881 caatcccaga cgggcggtga ccatattatc cagatcctgc ccgggcatac cgggagtccg
     2941 caagccctcg cggataccgt acgccaccga ccaggtgaaa tcgggccagg cctctctttt
     3001 ggcggcttta tactctgcct gcgctttgag gatgtcggcg cggagcatct tgagcatcgg
     3061 ccgggtttca gtcaaaccgg ccagaaggga gtcgcgattc ggggtataga gaaccggctt
     3121 tacctcttcg ggaataatat ccggcgtttc cggcccgcgg tctaaaagcg atttcagttc
     3181 attttgaacc gccactgtcc tcttctctaa gttggccaac ttctcctcca accgcgcctg
     3241 ctcgacggtg gccattaaga ggtcttgctg gagtcccgtg cccacttcgt agcgggtgcg
     3301 ggttaaggcg acaaaatcgg agaggatggt aatcgccttt tgggtcaact cctggcttct
     3361 gcgctgggcg aaaagttcga aataggtttc cttcacctgc cggatcaact cgagtttctg
     3421 ttcttcgtag ttccagcgcg ccacctccac tgcttggcga acgacgttct ttttcgtccc
     3481 gagcttcccg aagaagggaa acatttgtga gatgcctatc gacttcatcg tcatctcatc
     3541 ctcgcggaaa gagaaactgg tcggcaggtt ctccaccccc agcatcagtt ccgggtcggg
     3601 gaggtagccc acccgcgcca cctccgcctg cgccttttcg atattgcggg cggcgacccg
     3661 cagattggga ttcacgcgga ccgcttctgc aaccagctgc gccagaggcg tccgggtcgt
     3721 atcctgcgct ggggacacgc ccgggaaaaa ccctattgcc aggcaactga aaagtaagaa
     3781 tgaccctttc aacccactcc gaattctccg catagccgct ccttttccca ctccttgctc
     3841 aaaagaaatc taccatagcg gccgagccag ccgcaataga aacaatcacc ctctcgtgaa
     3901 aggtgacggc ttctctccac aatgaaaagc cgcccaatca gagttggatg agttttagga
     3961 aaggagcgtt ttaggaggtg gcggagaggg ttcgctggag cgcgagattg gctgagaatt
     4021 aactaccggc gaaacgagtc tatccaggag aagttttgtc tcggggagcg gaacggtggc
     4081 tgctaccgtc gttccctggc aagagcaacc caaaagacac ttgcagggat gcgtcgattt
     4141 ctctccggga tgcatgggac agggccgtgc gtgtgactgc tcctgcgccg cggcctggcc
     4201 ggtttgactg tggccctggc agcagggcat ctggtctttg gcctggacgg gagagctggt
     4261 gatctgaaca aaaaggacgg caaaaaagat tatggccagt ccagtcatac tgagcttgaa
     4321 tgtttttgtc agagttaatc ccatgctatt taccaatacg tgaaataggt cacttttact
     4381 atcctctatt ttctacatcg gctaaaagtc tctaaccttt tgcggtacag agggtagagc
     4441 aaacaactct gtcttgatga ctccccgcaa gtctccggct taacctaagc cgcgttgcgg
     4501 gagtttcagc ccggttttaa ggcaggtgcg gtgtcgccgg atgcaaagat ctccgccggg
     4561 gctatattca cccgttttgc tatttcctgc gataaatttt ttagttcctg cttgacaaac
     4621 gccacggtgg aagaaagcac ctggcccgtg ccgtcgatca agaaaatggt cggcacgatg
     4681 gaaatgccgt aggcattcga caccggatag ggtgcggttt cgggcaacac tggaaaagaa
     4741 agaccatact gacgcgcgaa ttctttcgac tggtcggccg ggtcctgctc aattccccag
     4801 aagcgaaccc ccttttgctg ataagcctgg tatagtcttt ccaggtaggg cacggcgagc
     4861 tggcaggtgg ggcagctgtt tttgaaaaac gagaggacgg tgagtgcgga ggtgccgtca
     4921 tctttgaatt cgtacggctt tccatcaatt cctttgagtt gaaaggtcgg tgcttttttc
     4981 cccgtggtta caggagccat atactttcct cctcgtgtct tctatcatac gtaagataaa
     5041 tcccgtctgc aagtccgcga ctactttgtt tctcgatgga ggaagtgccc caatcgccgt
     5101 acctgtcgtc tgaaaaagcc gggcactccc acgtcggcaa tcttatcggc gagagcttgt
     5161 cccgaaatcg tatcgggccg gaaggtcacg accacttgct ccgcggtctt tccccgctcc
     5221 acctttgaca cccccggtag cgcggccaga acctggcgcg tgtggctggc tcagagcccg
     5281 caggtaaagc cctccacctc cagagtcgcc tggacgagac cgccgggcca aggcgtctct
     5341 tttgaggcgg tgaccatgac ctttccgggg aaacctttca cgtgacaaac tcagacggca
     5401 acctcaatgt cgccattctg cacccgcact tcaaacttct g
//
LOCUS       DBNGZD010000031        12126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1532565, whole genome shotgun sequence.
ACCESSION   DBNGZD010000031 DBNGZD010000000
VERSION     DBNGZD010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12126
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1532565"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2265)
                     /locus_tag="AC16GM_00910"
     CDS             complement(<1..2265)
                     /locus_tag="AC16GM_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2922270.1"
                     /translation="MVEPALQARLARANPADTFRVLLVLADRVPTAKLNYELKATTKS
                     RAVRHQAVIEALKTKARVTQADLLTQLEREKQNGAIAAYKGYWIDNVIWVRGTKAIIE
                     RLINDPRIAEAYPELQPQLVEPVGPGEQAPLVPGGTEPGLRAIKADSAWLMGYTGRGR
                     LVCNFDNGVLGTHRAFSTRWRGANGAPASASWFDPTRNSAFPDTFFVGTNWHGTFTMG
                     IMCGRDPVSFDTIGVAINAQWIAAGVIDVSFTPTSYLALDAFEWAADPDGDPNTIEDV
                     PDVVNNSWGFPRVNPFSCAVDDSSFLIGCSNVFWGAIDNLEAAGCAVVFAAGNEGGCG
                     AGTLRNPANRASTPYNAFSVGATSPLTPFTIASFSSRGPSDCDGSIKPEVVAAGNGVR
                     SSGWPSGPRTNTSYGVSSGTSFSTPYVAGAVALLREFNPNATVDTIKYALLNSATDLG
                     TPGEDNAYGRGMINIKAALGLLPPNDSIHLFVKRDILTDLNGGTAEPGDTLALLLRLG
                     NSGADTSSGTWGRLFSTDPRVTVVAGFDSAFFGTIAPKDSAANATTLLRFAFALTAPL
                     NENLPLKLAVFTNAGPLDTLPLFFHTRDRLVRSIVTHSLANYFFTVSNFGQFGFGPGS
                     EIERGALGQGFFYPKAGGLNNLFEMALLMGTSASTVSNAARGDFSSILIDRHFVPIAG
                     GNLTRLTPGPQADEESFDRFNDSGAPRPLGVEITQNIMSFTAANYDDFAILEYRIKNV
                     SGGALNGLRVGLFAD"
     gene            2533..3777
                     /locus_tag="AC16GM_00915"
     CDS             2533..3777
                     /locus_tag="AC16GM_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR2922271.1"
                     /translation="MPAEIVTIGDELLSGQTVDTNAAFIGEKLMAAGIPVRWRTTVGD
                     AEADIAEALERAHKRAEVVIATGGLGPTHDDVTKKAIVQVFRRSLVFHDDVLKKVQAL
                     FSRRGLEMPKINQNQALLPQGTKIIDNPVGTAVGIWLEEDGRVFVALPGVPAEMRPML
                     LEGVLPLLRQEGRRFLLQRKLRTTGIPESALYERLAAKIAGEGEIRVAFLPGFLGVDI
                     RLSLLTANREHGQRQLETFVEALRPLIGPVLYGVDAETLEKVVGQLLAERKLTLAVAE
                     SCTGGMLGERITNISGSSKYFDRGVVTYSNQAKMDLLGVPAILIEKHGAVSAEVAGAM
                     AEGVRRAARTDFGLSITGIAGPAGGTAEKPVGLIYIGLAASSQTITRRFIFGTERDLN
                     RQRASQAALDLLRLYLLNEETA"
     gene            3774..4406
                     /locus_tag="AC16GM_00920"
     CDS             3774..4406
                     /locus_tag="AC16GM_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922272.1"
                     /translation="MKVAHDPAKLLEKYLILKDFSINEERAISERARNYLTTTAILFT
                     AYGLALQIAFLRYAIPIIGILYNFFWYYLGKRNALAFYYWRREMHLIEEEFQKIGQGY
                     EVYNIFSNRDRYYAGEPVATLSSDLVESRGESVRTDKIHQYIPGDGSRRPQVRLYAGQ
                     GIERQFPWRFLGNSLNFQMIYTPAFFLVAWAVVLGMSIFFPHFFLSGRVR"
     gene            4410..4967
                     /gene="thpR"
                     /locus_tag="AC16GM_00925"
     CDS             4410..4967
                     /gene="thpR"
                     /locus_tag="AC16GM_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR2922273.1"
                     /translation="MRTFIAVELPEEVKTRVAEVSAGLSKTGADVKWVELQNLHLTLK
                     FLGEVGQEKIEAVFEKTQHAAAGTKAFQLALESTGCFPNPKRPRVIWIGLSAGKTELV
                     QLAARLENEMASLGFPREDRPFAGHLTIGRVRSPKNLDRLLAAMNGTAFSHSGVTVDH
                     VVVMKSDLTPRGPVYSRLKTITLEP"
     gene            4976..6043
                     /gene="recA"
                     /locus_tag="AC16GM_00930"
     CDS             4976..6043
                     /gene="recA"
                     /locus_tag="AC16GM_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR2922274.1"
                     /translation="MPVEIAQEKRRALDQAVTQIERQFGKGAIMRMGEDHKVAVPVIS
                     TGSVALDSALGVGGVPRGRVVEIFGPESSGKTTLALHIIAEAQKAGGVAAFIDAEHAL
                     DTTYAKHLGVDLDNLLISQPDTGEQALEITETLVRSGAVDLVVIDSVAALVPKAEIEG
                     EMGDAHMGLQARLMSQALRKLTGTISKSKSCVLFTNQIRMKIGIMFGNPETTTGGNAL
                     KFYASVRLDIRRISTIKEGEQVVGSRTRVRVVKNKLAPPFKEAEFDITYGIGISREGE
                     LVDIGSDLKILEKSGTWYSFNGEHLGQGRDNTRQYLKDYPDVANKIAAKIRERLGLVK
                     VESASGGKEQPEKKEPAKAKK"
     gene            6123..6779
                     /locus_tag="AC16GM_00935"
     CDS             6123..6779
                     /locus_tag="AC16GM_00935"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR2922275.1"
                     /translation="MGQGVITKIARQKRARERVSLYVDGRFLCGLDEVAFHELHLKEG
                     DRLSSYSQQELKAQSVAGKAKDAALRLLAIRPRSRKELARRLQKKGYGAAEIESVLHQ
                     LQEENLLDDERFARLYAEHRLTSRHIGQRMLLAELLQKGINRTVAQSVVNEITGDDRE
                     KIEARVFAEKKLRLLSKGTALQRKKKLYDALSRRGFSPEVVREVLRNLLKKSMDEDVE
                     "
     gene            6793..9501
                     /gene="alaS"
                     /locus_tag="AC16GM_00940"
     CDS             6793..9501
                     /gene="alaS"
                     /locus_tag="AC16GM_00940"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR2922276.1"
                     /translation="MWTSHQIRTTFLSFFAERNHQVVRSSSLVPDNDPTLLFTNAGMN
                     QFKNVFLGLEKRDYAKATSVQKCLRAGGKHNDLENVGYTTRHHTFFEMLGNFSFGDYF
                     KDEAIALAWEFVTGVLQLPVERLFATVHNEDDDSFKIWLKVAPDLKGRVLRFGDKENF
                     WSMGETGPCGPCSEIHYDRGKEFGCGKPTCGVNCDCDRYVELWNLVFMQYNRDESGKM
                     TPLPKPSVDTGAGLERIAAVMQNVNSNFDIDTIRPIIAEIEKLTGKKYYPDERGLSHR
                     VIADHIRALTFAIADGIIPSNEGRGYVLRRIARRAIFYGRLLEFHEPFLTKLVQQVVT
                     PMRNTYEELQVKQKLVENFLFSEETLFAQTLEVGETYYEAKKRELKGKHSEVFPGVTV
                     FNLKDTYGFPEDLTAIMAKRDGLEIEWDSFHVELENQRKRSKTIVSSIGISSSETFGT
                     PLIINWKRFEKIPLTEFDYENLEVSAKITEADQIQSKGDEVFCVSLDKTSFYFESGGQ
                     ISDTGKIESEVDPLLMDVVSLQISPSHKKIHVVRPRQGQAGLVKKDIPVKTRINIPRR
                     RAIMRNHTATHLVHKALREVLGEHVHQAGSLVADDRLRFDFTHFKQMSPEELKAIEEQ
                     VNEKILENLKVWYDTKSIEYAKNVMKAMALFGEKYGDNVRVIGIGGDKENFGQAYSIE
                     LCGGTHTKATGEIGLFKIFSESGIAAGVRRIEAVTGLGLYELTRRQGEQIAQLANLAK
                     AMPEQLAQKVTELLDENRTLQKQLEEFKKKSAGSVAEQLLAEAQSVNHVKVVTKQMEA
                     SSRDDLLHLADQFTVLKEPVVSVLAAQVDGNIIFVGTASPAAIGEKGLKAGDIIREVA
                     KIAGGSGGGKPHLAQAGSKDPSKVASALEAVPGIVQKFMQGAKGRA"
     gene            9498..10238
                     /locus_tag="AC16GM_00945"
     CDS             9498..10238
                     /locus_tag="AC16GM_00945"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HIR2922277.1"
                     /translation="MNVYTRLTEKLKTQKGLFFVLLDPDRQSPDDLAASALLAQEAGA
                     DALLAGSSLLVTAQFNGALRDLKTQVKIPVILFPANAYQVCPHADAILFLSLLSGRNP
                     QYLIGEQVNGAPLVKAFGLEPIPTGYLLIESGKPTSVAYMSGTQPIPADKPDIAKAHA
                     LAAEYFGMKFVYLEAGSGAVSAVPDALIRAVRDYVSVPLIVGGGIRSPEVARQKVVAG
                     ASAVVVGHALEQRTAPKILREFADAIHG"
     gene            10247..10834
                     /locus_tag="AC16GM_00950"
     CDS             10247..10834
                     /locus_tag="AC16GM_00950"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR2922278.1"
                     /translation="MTTAQRLAAKSLRASITVKLDLLEHHLPRIVSLGNRLTHCLKRG
                     RKILICGNGGSAADAQHFAAELVVRLKSKRRALPCLALTTDTSTLTACGNDFGFEEIF
                     ARQVEALGARGDLLVLLSTSGRSANILRAARSAKKRGLVTVALTGSRRNPLTRTAAFA
                     LQVPSTDTQRIQEAHITILHLWGELIERNLKRPGR"
     gene            10834..12102
                     /locus_tag="AC16GM_00955"
     CDS             10834..12102
                     /locus_tag="AC16GM_00955"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR2922279.1"
                     /translation="MPQSSQVYEWLGKAGLFGFCLFSPFSIAGAEISLGIALFGWLAS
                     ALFNRSLAWRHTVLDWAVSVLLLAVLASAFFGVDPKRSLVSLKDFFPLTLLFVALNFP
                     KDPRSAERLLTLLAAAVSVAALNGIYQHFSGMDWIHHGPLNPTRLGFRAQGTFSTIMT
                     YSEYHLLAGLFFLTLAMSPSAPFKRILYGMAGQLSVVALLFSYARGAFLALAGGLLAY
                     FFLAPRGKKFFPVAFTVAVGLVTYLASPDLISRFEDLARTGRDESVGNSRLDIWQTTL
                     AIFADHPVTGIGFGNFETLYDRYRTNRAGRILGHAHNDWLNFLVHSGVVGLAAFILFW
                     LAVLRVLWQGYRHAGDQARFYPLAALVAGCGYLVVSQIEAVFFDEEVLLLLCFLVGIG
                     LRGVAGKAEASDTSSTRAGAGLISPGKTAY"
BASE COUNT         2695 a         3315 c         3564 g         2552 t
ORIGIN      
        1 atcggcaaaa agcccgaccc gcaggccgtt gagcgcaccg ccggatacgt ttttgatccg
       61 atactccagg atagcgaagt catcgtagtt ggcagcggta aaggacatga tgttctgggt
      121 aatctccact cccaaaggcc gcggcgcgcc ggagtcattg aatcggtcaa aagactcttc
      181 gtccgcctga gggccgggcg tcaggcgcgt cagatttccg ccggcaatcg gcacaaaatg
      241 gcggtcaatg agaatggagc tgaagtcacc ccgcgcggcg ttagagacgg tgctggcgct
      301 ggttcccatt aaaagcgcca tttcaaacag gttgttcaat ccgccggctt tggggtagaa
      361 aaacccctgg cccagcgcgc cgcgctcgat ctcagaacca gggccaaagc caaactgtcc
      421 gaaattagaa accgtgaaga agtagttcgc cagtgagtgg gtgacgattg aacgcaccag
      481 acggtcccgg gtatggaaga ataagggcag cgtatccagc ggcccggcat tggtgaagac
      541 ggccaatttc aaggggagat tttcgtttaa gggtgcagtc agggcaaaag cgaagcgcaa
      601 aagagtcgtc gcgttcgcgg cgctgtcttt gggggcaatg gtgccgaaga acgcagagtc
      661 aaagccggct actactgtta cccgcggatc ggtcgaaaag aggcggcccc aggtgccgga
      721 cgaagtatcc gcgccggagt tccccagccg gagcaaaagg gcgagggtat cgcccggctc
      781 ggccgtcccc ccgttcaggt cggtcaggat gtctcgcttc acgaacaggt gaatgctgtc
      841 gttggggggg agcagcccca gggcggcttt aatattaatc atgcccctgc cgtacgcgtt
      901 gtcctcgccg ggggtaccca gatcagtcgc ggaattcaaa agggcgtatt taatcgtatc
      961 gacggtggca ttggggttga attcacgcaa gagggccacg gctccagcta cataaggtgt
     1021 ggaaaaagaa gtcccggagg agactccata agaagtgtta gtgcgcggtc ccgaaggcca
     1081 gccggaagag cggacgccat tccccgcggc cacgacctcc ggctttatgg agccgtcgca
     1141 gtcggagggg ccccggctgg agaaagaggc aatcgtaaaa ggtgtgaggg gagaagtagc
     1201 cccgactgag aaggcattgt aaggagttga ggcgcggttg gccgggttgc ggagcgtccc
     1261 cgccccgcag ccgccttcgt tgccggccgc aaaaaccacc gcacagccgg cggcttcgag
     1321 gttgtcgatg gcaccccaga agacattgct gcaaccaatc aagaaactcg aatcatcaac
     1381 cgcgcaactg aacgggttga cgcgggggaa accccaggag ttgttcacca cgtcgggcac
     1441 atcttcaatg gtgtttgggt cgccgtcggg atcagcggcc cactcaaaag cgtccagggc
     1501 gaggtagctc gtgggcgtga aactgacgtc gatcactccc gccgctatcc actgagcatt
     1561 gatagccaca ccaatggtat cgaaggatac cggatcccgg ccgcacataa tccccatcgt
     1621 aaaagtgccg tgccagttgg tgcctacaaa aaaagtgtcc gggaaggcgg agttccgtgt
     1681 tgggtcgaac caagaagcgg aggccggcgc gccgttagct ccccgccagc gggtgctgaa
     1741 ggcacgatgg gtgcctaaga ctccattatc gaaattacac actaagcgtc cgcgaccggt
     1801 gtaacccata agccaggcgg agtcagcctt gatcgcccgc agtcccggct cagtcccgcc
     1861 gggaactaaa ggagcttgtt ccccggggcc caccggctcc actaactgcg gctgcagttc
     1921 cgggtaggcc tcggctatcc ggggatcgtt gatcaaccgc tcaataatcg ccttcgtccc
     1981 ccgcacccag atgacgttat caatccagta ccccttgtag gcggcaattg ctccgttctg
     2041 tttctctcgt tctaattgcg tgagcagatc ggcctgggtg acccgggctt tggttttgag
     2101 ggcttcgatg acggcttggt gtcgcaccgc ccggcttttg gtcgtggctt tgagttcgta
     2161 gttcagtttg gccgtaggca cgcggtcggc caataccagg agcacacgga aggtatcggc
     2221 gggattggcc cgggccagcc gggcctgtaa ggccggttcc accattgccc cctcaaggag
     2281 tgacgtactg acgaagaaac aaaaaatccc cgtacctacg attcctgtcc ctctgcgcat
     2341 cggcgacctc acgtttgggt ttctttcagt tcaaatcgaa acggcgcacc aatatagtga
     2401 accggccaaa cgagggcaaa gtttccctgc cttgactttg aaagtgattc gggcatattt
     2461 ctcttcgggt aggacgaaga cctttgttga aagcattctt cggaagagga tgggttaccc
     2521 acgcaagttc ctatgcctgc tgaaattgtc accattggcg atgaactctt gtcgggacaa
     2581 accgtcgata cgaatgccgc ttttatcggt gaaaaattga tggcggcggg cattccggtg
     2641 cgctggcgga ccaccgtggg cgacgccgag gccgatattg ccgaggcatt ggaacgtgcc
     2701 cacaaaaggg cggaagtggt gattgccacc ggtggcctgg gccccacgca cgacgacgtc
     2761 accaagaaag ccattgtgca ggttttcagg cggagcttag tcttccacga cgacgttttg
     2821 aaaaaagtcc aggcgctgtt ctcccggcgc ggtttggaaa tgcccaaaat caaccagaat
     2881 caagccctgc taccgcaggg gacgaagatc atcgacaacc cggtcggcac cgcggtgggg
     2941 atctggctgg aagaagacgg ccgggtcttt gtggcgctgc ccggcgtgcc ggcggaaatg
     3001 cggccgatgc ttttggaagg cgtactgccg cttttgcgcc aagagggccg gcggtttctc
     3061 ctccagcgca aattacggac gaccggtatt cccgaatcgg cgctttacga acggctggcg
     3121 gccaaaatcg ccggggaggg cgaaatccgc gtggctttct tgcccggttt tctcggggtg
     3181 gatatccgcc tttcactttt gactgcaaat cgagagcacg gccagcgcca gttggaaacg
     3241 ttcgttgaag cgctcaggcc cttaatcggc ccggtccttt acggggttga cgctgagacc
     3301 ttagagaaag ttgtgggaca actgctggcc gagcggaaat tgactttagc cgtggccgag
     3361 tcgtgcaccg gcgggatgct gggagagcga atcactaaca tttcgggtag ttcgaaatat
     3421 tttgaccgtg gggtggtaac ctacagcaat caggcgaaaa tggacctttt aggcgttccc
     3481 gccatactta tcgaaaagca cggggcggtc agcgcggaag tcgccggagc gatggcggag
     3541 ggcgtgcgcc gggcggctcg aaccgatttc ggtctttcta taaccggcat tgccggcccc
     3601 gcaggcggca ctgcggaaaa gccggtcggg cttatctaca tcggtctcgc ggccagcagc
     3661 cagaccatta cccggcgttt cattttcggc accgaacggg acttgaaccg ccagcgggcc
     3721 agccaagcgg ctctcgactt gctccggctc taccttctca acgaggaaac ggcatgaaag
     3781 tcgctcacga tccggccaag cttttagaga aatacctgat tctgaaagac ttttccatta
     3841 acgaagagcg ggcgatttcg gaacgtgcgc gcaactacct gaccaccacg gcgatccttt
     3901 ttaccgccta cggtctggcg ctgcaaatcg ccttcctccg ctacgccatt ccgatcatcg
     3961 ggattctcta taacttcttc tggtactatc tgggcaagcg taacgccctg gctttctact
     4021 actggcggcg cgagatgcat ttgattgagg aggagttcca gaaaatcggc caggggtacg
     4081 aggtctacaa catattttcc aaccgcgacc gctactatgc cggagagccg gtggctaccc
     4141 tctcttccga tctggtggag agccgggggg agagcgtccg gaccgataag attcaccagt
     4201 atattccggg cgacggcagc cggcggcccc aggtgcggct ctacgcggga cagggaattg
     4261 agcggcagtt cccgtggcgt tttttgggga actcgctcaa ctttcagatg atttacaccc
     4321 ccgctttttt cctggtcgcc tgggcggtgg tgctggggat gagcattttc ttcccgcact
     4381 ttttcctgtc gggccgggtc aggtgagccg tgcgcacctt tatcgccgtt gaactgcccg
     4441 aagaagtgaa aacccgggta gccgaggttt ccgccggcct cagcaagacc ggcgccgacg
     4501 tgaaatgggt tgaactccag aacctgcatc tgacgttgaa gtttttgggg gaggtcggcc
     4561 aggagaagat cgaagcggtg tttgaaaaaa ctcaacatgc agcggcaggt accaaagcgt
     4621 tccaactggc cttggagagc accggctgtt tccccaatcc caaaaggccg cgggtgatct
     4681 ggatcggact ttctgctggc aaaaccgagt tggtgcagtt agccgcccgc ctcgaaaacg
     4741 aaatggcctc cttgggattt ccccgggaag accggccttt tgccggacat ttgaccatcg
     4801 gccgggtgcg ctcgccgaaa aatttagata gattactggc agcgatgaat gggacggctt
     4861 tcagccatag cggggtgacg gtggatcacg ttgtcgttat gaaaagcgac ctgacgcccc
     4921 gcgggccggt ttacagccgc ctgaaaacga ttaccttaga accgtagagg gacttatgcc
     4981 ggtagaaatc gcccaggaaa aaaggcgcgc cttagaccag gcggtgaccc agattgaacg
     5041 gcagttcggc aagggcgcga tcatgcgcat gggggaagat cataaggtcg cagtgccggt
     5101 tatttccacc ggctcggttg cccttgactc tgccttaggc gtggggggag tcccccgcgg
     5161 acgggtcgtg gagatcttcg gcccggaatc atccggcaag accacgctcg cgctccatat
     5221 cattgccgaa gcgcagaaag caggcggcgt ggcggccttc atcgatgccg aacatgcgct
     5281 ggatacgacc tatgccaaac atctgggcgt cgatctcgac aacctcctta tctcccagcc
     5341 cgacaccggc gagcaggcgt tagagattac cgaaactttg gttcgctccg gcgcggtcga
     5401 tctggtcgtc atcgattcgg tggcagcctt agtccccaaa gccgaaatcg aaggcgagat
     5461 gggcgacgcc cacatggggc ttcaggcgcg cttgatgtcc caggcgctcc gcaagctgac
     5521 cggcaccatc agcaagtcga agagctgcgt tttgttcacc aaccagatcc ggatgaaaat
     5581 cgggattatg ttcggcaacc cggaaaccac caccggcggg aacgcgctca agttctacgc
     5641 ttcggtgcga ctggatatcc gccggatttc aacgatcaaa gaaggcgagc aggtcgtggg
     5701 ctcgcgcacc cgggtgcggg tggtcaagaa taaactggcc ccgcccttca aagaagcgga
     5761 gttcgacatc acctacggca tcggcatttc ccgcgaaggg gaattggtcg atattggctc
     5821 cgacctgaaa attctggaaa aatcgggcac ctggtactcc ttcaacggcg agcatctcgg
     5881 gcagggccgc gacaatacgc ggcagtacct caaggactac cccgatgtgg ccaataaaat
     5941 tgccgccaag attcgcgagc ggctggggct ggtcaaagtc gaatccgcct caggcggaaa
     6001 ggaacagccg gagaagaaag aaccggctaa ggccaaaaag tagccagaga ccataaacca
     6061 gtggccagcc gaagcggagc gaaggccccg ccaaaggcgg ggtagccagc actgaaagag
     6121 atgtgggtca gggtgtcatt acgaagattg cccggcaaaa acgtgctcga gagcgtgttt
     6181 cgctttacgt tgacggccga tttctttgtg ggctggatga agttgcgttc cacgaactac
     6241 atctgaaaga gggcgatcgt ctttcctctt acagtcagca ggaactcaaa gcccagagcg
     6301 tggcggggaa ggccaaagac gccgcgctac ggcttctggc catccggccc cgcagccgga
     6361 aagaattagc ccggcggttg caaaagaaag gatatggcgc cgctgaaatt gagtcggttc
     6421 ttcatcaatt acaagaggaa aacctgctgg acgatgagcg tttcgcccgg ctgtacgccg
     6481 aacaccgcct gaccagtcgg catatcggcc agcggatgct tctggcggaa ttgctgcaga
     6541 aaggaattaa ccgcacggtc gcgcagtcgg tcgtaaatga gataactggt gatgaccgcg
     6601 aaaaaatcga ggcacgtgtg tttgcggaga agaaattgcg gttgctttcg aaaggcacgg
     6661 cgctccagcg gaagaagaaa ctctacgatg cgctttcccg gcggggattt tcgcccgagg
     6721 tcgtgcgcga agttcttcgc aatctgttaa aaaagtctat ggatgaagat gtcgaataaa
     6781 ccgtaaagag ttatgtggac gagtcaccaa atccgcacca ccttcttgag ttttttcgcc
     6841 gagcgcaacc accaggttgt gcgctcctcc tcccttgttc cggataacga cccgacgctc
     6901 cttttcacca acgccgggat gaatcagttc aaaaacgtct ttttaggact ggagaagcgg
     6961 gactacgcca aagcgaccag cgttcagaag tgcctgcggg ccgggggcaa gcataacgac
     7021 ttagagaacg tcggctacac tacccggcac cacacctttt ttgaaatgct gggaaatttc
     7081 tccttcggcg actacttcaa ggacgaagcg atcgccctgg cctgggagtt cgtcaccggc
     7141 gtgctgcagc tgccggtgga gaggcttttc gccaccgtgc ataacgaaga cgatgattcg
     7201 ttcaaaatct ggctaaaagt agctccggac ttgaaaggcc gcgtcctgcg ttttggggat
     7261 aaagaaaact tctggtcgat gggcgaaacc ggcccctgcg gcccctgttc ggaaatccac
     7321 tacgaccggg ggaaagagtt cggctgcggc aagcccacct gcggggtcaa ctgcgattgc
     7381 gaccgctacg tggaactctg gaatttagtc tttatgcagt ataaccggga cgaatcgggg
     7441 aagatgaccc cgcttcccaa accctcagtg gataccggtg cgggactgga acgaattgcg
     7501 gcggtgatgc agaacgttaa ttccaatttt gatattgaca cgatccgtcc catcattgcg
     7561 gagatcgaaa aacttactgg gaaaaagtat taccccgatg aacgcggact atctcaccgg
     7621 gtgattgctg accacattcg ggctttaacc tttgcaattg ctgacggcat tattccttca
     7681 aatgagggca gagggtacgt cctgcgtcgc atagcgagga gagcaatatt ttacggcaga
     7741 cttctggaat tccacgaacc ttttttaacg aagttagtcc aacaggttgt gacgccgatg
     7801 aggaatacct atgaggaact ccaggtaaag caaaaactgg tagagaactt tctcttttct
     7861 gaggagactc tttttgcaca aaccctggaa gtcggcgaga cttactatga ggccaagaag
     7921 cgagaactca agggtaaaca ctctgaagtc tttcccggtg tcacagtttt taatctgaaa
     7981 gacacatatg gatttccgga agatttgacc gccataatgg ccaaaagaga tggtttagaa
     8041 atagaatggg atagttttca cgtagaattg gaaaatcaaa ggaaaagatc gaaaacaata
     8101 gtaagctcaa ttggtatttc ttcatcagag acgttcggca caccgctaat tatcaactgg
     8161 aagcgatttg aaaaaattcc tcttacggag ttcgactacg aaaatttgga ggtttcggcg
     8221 aagatcaccg aagccgatca gattcaaagt aagggagatg aggtattctg tgtttctctt
     8281 gataagacct ctttttattt tgaatcaggt ggacaaatca gcgataccgg taagattgag
     8341 agtgaagtag atccactttt gatggatgtt gtaagtctcc aaattagtcc ttcccataaa
     8401 aaaatccatg ttgttagacc tcggcaggga caggcaggtc ttgttaagaa agatattccg
     8461 gttaagacga gaatcaacat cccccgccgg cgagccatta tgcgcaacca cacggccacc
     8521 catttagtcc acaaggccct gcgcgaggtt ttaggcgagc atgtgcacca ggccgggtca
     8581 ctggtggctg atgaccgctt gcggttcgac ttcacacact tcaagcagat gagtcctgaa
     8641 gaactcaaag ctatcgaaga acaggttaac gaaaaaattc tggagaacct taaagtctgg
     8701 tatgatacca agtccattga gtatgcaaaa aatgtgatga aagcgatggc actttttggt
     8761 gagaagtatg gagacaacgt gcgggtaatt ggcatcggtg gtgacaaaga aaattttggt
     8821 caggcttact ctattgaact ctgcgggggg acgcatacga aggccacggg cgaaatcggg
     8881 ctgttcaaaa tcttttctga aagcggcatt gcggcggggg tgcgtcggat tgaagcagtt
     8941 accggattgg ggctctatga attgacccgc aggcagggtg agcaaattgc ccagctggcc
     9001 aatctggcca aagcgatgcc ggaacagtta gctcagaaag tgactgaact cctcgatgaa
     9061 aaccgcacgc tgcaaaagca acttgaggaa ttcaaaaaga aatcggccgg atcggtggcg
     9121 gaacagctct tggccgaagc gcagtcggtc aaccacgtca aggtcgtcac caaacaaatg
     9181 gaggcatcca gccgcgacga cctgctccac ctggctgacc agttcactgt gctgaaagag
     9241 ccggtcgtca gcgttctggc tgcgcaggtg gacggcaata ttatattcgt cgggacggcg
     9301 tcaccggcgg cgatcggcga aaagggactc aaagccggag atatcatccg cgaggtggcg
     9361 aaaatcgcgg gcggctcggg tggcggcaag ccgcacttag cccaggccgg gagcaaagac
     9421 ccgtcgaaag tagcgagcgc attggaagca gtgccgggaa ttgttcagaa gttcatgcag
     9481 ggagcaaaag gacgcgcgtg aacgtttaca cgcggctgac agaaaaactc aaaacccaga
     9541 agggactctt cttcgtcctg ctcgatcccg accgccagtc gccggatgat ttggccgctt
     9601 ccgcgctttt ggcgcaagaa gcgggtgcgg atgccctttt ggccgggtca agtcttttgg
     9661 tcaccgccca gttcaacggc gctctgcgcg atttgaaaac acaggtcaaa attccggtga
     9721 ttcttttccc cgccaatgcc taccaggttt gcccgcacgc cgatgcgatt ctgttcctct
     9781 cgcttctctc gggccgcaat ccgcagtacc tcatcggcga gcaggtgaac ggcgcgccgc
     9841 tggtaaaagc cttcgggctg gagcctatcc ccaccggata tttgttgata gaatcgggca
     9901 agcccacctc cgttgcctat atgagcggaa cccagccgat tccggcggac aaaccggata
     9961 ttgccaaagc ccacgcgctg gcggcggagt atttcggaat gaaattcgtc tacctcgaag
    10021 ccggttccgg cgcagtttcc gccgtgcccg atgcgctcat ccgggcggta cgcgactatg
    10081 tctcagtccc gctcatcgtc gggggcggaa ttcgctctcc ggaagtggcc cggcagaagg
    10141 tggttgccgg cgcttctgcc gtggtagtcg gtcatgctct ggagcaacgc acggctccca
    10201 aaatcctccg cgagtttgcg gacgccattc acgggtagtt ccccggatga ccaccgcaca
    10261 gcgtctggcg gcaaaatccc tgcgggcgag cattacggtc aaacttgatt tgctcgagca
    10321 tcaccttccc cgaatagttt ccttaggaaa tcggttgacc cattgtctca agcggggacg
    10381 caagattctc atctgcggca acggcggctc ggcggcggac gcccagcact ttgccgctga
    10441 attagtggtt cggctcaaaa gcaagaggcg ggcgctcccc tgtttggcac ttaccaccga
    10501 cacctcaacc ctcaccgcct gcgggaatga cttcggcttt gaggaaattt ttgcccggca
    10561 ggtggaagcg ctgggcgccc gcggtgacct tctggtgtta ctctccacct ccggcaggtc
    10621 ggccaatatc ctccgtgccg cccggtcggc aaaaaagcgc ggcttggtga cggtggccct
    10681 caccggcagt cgaagaaatc ctctcacgcg gacagccgct ttcgctctcc aagtgccgtc
    10741 cactgatact cagcgcattc aggaagccca catcaccatt ctgcacctct ggggcgaact
    10801 gattgaacga aatctgaagc gtccgggccg ttaatgcctc aatccagtca ggtttatgaa
    10861 tggttgggca aagccggtct tttcggtttc tgcctttttt cgcctttttc catagccggc
    10921 gccgagatca gtttaggcat tgcgctcttc ggctggctcg cttccgccct gtttaaccgg
    10981 tcactggcct ggcggcacac cgtcctcgac tgggcggtgt cagtattgct tttggcggtt
    11041 ctggccagcg cgtttttcgg ggtagatccc aaaaggtctt tggtgtcgct gaaagatttt
    11101 ttcccgctga cgctcctttt tgtggcgctg aacttcccca aagacccccg ctctgccgag
    11161 cgcttgctca ccttgctggc cgcggcggtg agcgtggctg cgctgaacgg catctaccag
    11221 cacttttcgg gcatggactg gatacatcat ggcccgctta accccacccg gctcgggttc
    11281 cgcgcccagg gcactttcag cacgattatg acttacagcg aataccatct tctggcgggt
    11341 ctattttttt tgactctggc gatgagtccg tcggcgccct tcaaacgcat cctctacggg
    11401 atggccgggc agttgagtgt cgtggcgctt ctcttcagtt acgcacgggg cgcgttcctc
    11461 gccctggcgg gcggtctttt ggcatatttt ttcctggctc ccagagggaa gaaatttttt
    11521 ccggtggctt ttaccgtggc agtcggcttg gtgacctacc ttgcttcgcc agacctcatc
    11581 tcccgttttg aagatttagc ccgcaccggc cgggacgaga gtgtcggcaa ttcgcgcctg
    11641 gatatctggc agaccacgct ggccatcttt gccgaccatc ccgtcacggg catcggtttc
    11701 ggcaactttg aaacgctcta cgaccggtac cgcaccaacc gggctggaag gatcctgggc
    11761 cacgcccata atgactggct gaacttcctc gtccattccg gggtcgtggg actggcggcc
    11821 tttatccttt tctggctggc tgtattgcgt gttttgtggc aagggtaccg ccacgccggc
    11881 gaccaggccc gcttctatcc cctggcggcg ctggtggccg gctgtggcta tctggtcgtc
    11941 agccagatcg aggcggtatt ttttgacgag gaggtcctat tgcttttgtg cttcctcgtc
    12001 ggtatcggct tgcggggagt ggcgggcaaa gctgaggcgt ccgatacctc cagtaccagg
    12061 gcaggtgcgg gcttgatttc gccggggaaa accgcatatt gagaggcaaa catggcgcag
    12121 aaaccc
//
LOCUS       DBNGZD010000032         5090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_213219, whole genome shotgun sequence.
ACCESSION   DBNGZD010000032 DBNGZD010000000
VERSION     DBNGZD010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_213219"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16GM_00960"
     CDS             complement(<1..200)
                     /locus_tag="AC16GM_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR2922280.1"
                     /translation="MPKLVDYTVQDGLAILELNDPPANTYTHEMMRDLDDSILQARFD
                     EKVHVIILRGKDDKFFSAGANI"
     gene            complement(477..752)
                     /locus_tag="AC16GM_00965"
     CDS             complement(477..752)
                     /locus_tag="AC16GM_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HIR2922281.1"
                     /translation="MPKYVIERDIPGAGKLSEKELQAISQKSCSVLSAMGPQIQWMHS
                     YVTDNKIYCVYIAPNEQAVREHAQKGGFPANRVSEVRTMIDPTTAES"
     gene            complement(818..1579)
                     /locus_tag="AC16GM_00970"
     CDS             complement(818..1579)
                     /locus_tag="AC16GM_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR2922282.1"
                     /translation="MLPVPLLTIVLIGIFILANAIRILREYERGIIFRLGRLRGAKGP
                     GIIFLIPIVDKMVKVSLRVVAMDVPSQDVITRDNVSVKVNAVIYFRVIDASKAIVAVE
                     DFLYATSQISQTTLRSVLGQFELDDLLANREKINHQLQQIIDGQTEPWGIKVSVVEVK
                     NVDLPQEMQRAIAKQAEAERERRAKVIHAEGELQASYKLAEAAEVIGKQPAALQLRFL
                     QTLVEVAADKNSTTVFPVPIDLIKPFMSLAERGGK"
     gene            complement(1658..2983)
                     /locus_tag="AC16GM_00975"
     CDS             complement(1658..2983)
                     /locus_tag="AC16GM_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR2922283.1"
                     /translation="MKIKRSLSTVLCLVAASLLLAQAQEKKASPPGEGTHEVYLLTLE
                     GAITPVTAKLITQAIDKAEEKQAEALVIQLNTPGGLVLSTWQITKDILNADVPVIVYV
                     APSGARAASAGMYITYAAHIAAMASGTNIGAAHPVELQGGKADSTMMEKATNDAVANV
                     KSIATKRHRNEKWAEDAVRKSVSIPEYEALKLNVIDLVADNLSDLLAKCDGRTAELPI
                     GPRTLKTKGAPIRKVTIGPIDRILEVIVDPTIAYILFTIGMLGLYFEFANPGAIVPGV
                     VGGICLILAFYAFQSLPINYAGVLLIVLAMVLFILEVKVPSHGALTIGGIVAMLLGSL
                     LLITTSAPYARISLSVIITTVGATAAFFIFAVGAGLRAQRLKVTTGWEGMVGAIGVAH
                     SRFEPAQGGEGQIFVSGEIWRARASEPIEPNHKVKVTGVENLTLIVTRA"
     gene            3192..4544
                     /gene="glmM"
                     /locus_tag="AC16GM_00980"
     CDS             3192..4544
                     /gene="glmM"
                     /locus_tag="AC16GM_00980"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR2922284.1"
                     /translation="MKKQSSQLMVSVSGVRGVVGETLTPEVIVRYGLAFGQFVKRKKV
                     VIGRDARPSGPMVQNLAAGALLSTGCDVVDIGLTTTPTLELAVTDLKASGGVMVTASH
                     NPAEWNALKFFNASGTFLTPQQTKGVFDRAKKMHPGVKWNWAGKLSFDASRNAKHIEQ
                     ILGLPLINAFAIQKRRFRVVVDCVNGSGGVVVPELLARLGCEVTPLFCEPTGLFPHNP
                     EPVPKHLTALAQKVKETGADLGFAVDPDVDRLALVTEKGDAISEEYTLALAADLVLSK
                     TPGRVVVNLSTSRMVEDVAKKHGMPCYRTPVGEANVVLGLKKNKGVVGGEGNGGVILP
                     ALHYGRDALVGMALILQLLVERQKTLNQLVSELPAYQMEKIKKPLPAGFEKRLKKLAL
                     AFDGTRPDWRDGLRLDLPEGWLQVRKSNTEPVCRIVAEGQSEKSVNRILKEAQKVLQS
                     "
     gene            4624..>5090
                     /locus_tag="AC16GM_00985"
     CDS             4624..>5090
                     /locus_tag="AC16GM_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR2922285.1"
                     /translation="MCGIVGYVGEQKAVPLLLEGLKRLEYRGYDSAGIAILENGELFS
                     TKAAGKIAVLEKVLNGREFAATSGIAHTRWATHGEPTDLNAHPHVDCKDEIAVIHNGI
                     IENFAALKAVLERQGHKFTSETDTEVLAHLIEEHYEGNLLEATRAALSLVEGT"
BASE COUNT         1125 a         1336 c         1493 g         1136 t
ORIGIN      
        1 ttgatgttcg cgccggcgga gaaaaacttg tcgtctttcc cccgcagaat gatgacgtgg
       61 actttctcgt caaaacgcgc ttggaggatc gagtcgtcca agtcgcgcat catctcgtgg
      121 gtgtaggtgt tggcgggggg atcgttcaat tctaaaatcg ctaggccgtc ctgaacagtg
      181 tagtcaacta atttgggcat aatcactcct ttgcactggt taccgacatt tggtcgcagc
      241 aggaatgtaa cgctattgaa cagggagggt caagggcgga cagtaagccg ggttacggat
      301 tgaacgggtg taaaaaaccc gttgctggat gggtctggcg aaagggaata gtaagtgtgt
      361 ccggtagggt ctccggttaa tcaattctct tacgtcgttc cgaggaattg tctgtgggac
      421 gagaaaatat tcctccccgg acgaatgccc gcaccgttta gaaaccgaaa accgtctcag
      481 gattcggcgg tagtggggtc aatcatggtg cgcacttccg aaacccggtt ggccgggaag
      541 ccgcccttct gggcatgctc ccggacggcc tgttcgttgg gcgcaatgta gacgcagtaa
      601 atcttgttat cggtgacgta actgtgcatc cactgtatct gcgggcccat agcgctcaag
      661 actgagcagg atttctggga aatggcctgg agctctttct cggatagttt tccggcgccg
      721 ggaatatcac gttcaatcac gtacttgggc atcgtgtcct cctctgtttt acgcccacaa
      781 gtggctgggt taccaagtca gatgcgcggg tttcgggcta cttccctccc cgttccgcca
      841 atgacatgaa cggtttgatc aggtcgatgg ggacggggaa aaccgtcgtc gaattcttgt
      901 cggcggcaac ttcaactaaa gtttgcaaaa agcgcagttg cagggcggcg ggctgcttgc
      961 cgatcacctc ggccgcttcg gccaatttgt aggaggcctg gagttcgcct tcggcgtgga
     1021 tgaccttggc gcggcgctcg cgctccgcct cggcctgctt ggcaatcgcg cgctgcattt
     1081 cctggggcag atcgacgttc tttacttcta ctacggagac tttaatgccc cagggttcgg
     1141 tctggccgtc gatgatttgc tgcaactggt ggttgatttt ttcccggttg gccagaaggt
     1201 cgtcgagttc aaattgtccc agtacggaac gcaacgtggt ctgcgaaatc tgggaggtgg
     1261 cgtagaggaa gtcctccacc gccacgatgg ctttggaggc gtcaatcacc cggaagtaaa
     1321 tcaccgcgtt gaccttgacc gaaacgttat cccgggtgat tacgtcctgg gagggaacgt
     1381 ccatggccac cacccgcagg gagactttga ccatcttatc gactatgggg atcagaaaaa
     1441 taattcccgg gcctttggct ccgcgcaagc gccccagccg aaagataata ccgcgttcgt
     1501 actcgcgcag gatgcggatg gcattggcga ggatgaagat gccgatcagg acaatcgtta
     1561 ataacgggac gggaagcatt gttcctccgt gatgggttta agaagttttc agtaccaagt
     1621 tatcgtctgt ttcgttttgc cagttcgaat gcgtgactca ggcacgcgtt acaatcagcg
     1681 tcaggttttc tactccggtt accttaacct tatggttggg ctcaatcggt tcgctggctc
     1741 gggcgcgcca gatttccccg gaaacgaaaa tctgtccttc gccgccctgg gccggctcaa
     1801 atcttgaatg tgccacgcca attgcaccaa ccattccctc ccaaccggtg gtaactttca
     1861 gccgttgggc gcgcaatccg gcacccacgg cgaaaatgaa gaaagcggcg gtggcgccga
     1921 ccgtggtgat aatcaccgaa agcgaaatcc gggcgtaggg agccgacgta gtaatcagca
     1981 gaagcgatcc caaaagcatg gccacaattc ccccgatggt caaagccccg tggctgggga
     2041 ctttgacttc gagaataaag agcaccatag ccagaacgat aagcagcaca ccggcgtagt
     2101 tgatgggcaa actctggaag gcgtagaaag ccagaatcaa gcaaatccca cccaccaccc
     2161 cgggcacaat ggcgccggga ttggcaaact caaagtagag ccccagcatt ccgatggtga
     2221 agagaatgta ggcgatggtg ggatcaacaa tcacttcgag gatacggtca atggggccga
     2281 tggtcacctt gcgtatgggc gcgcctttgg ttttcagggt gcgtgggccg ataggcaatt
     2341 ccgccgtgcg gccgtcgcac ttggccagaa ggtcggacaa gttatcagca accaggtcga
     2401 ttacattcaa tttcaaagct tcgtattcag gtattgagac cgatttgcgg acggcatcct
     2461 cggcccattt ttcgttgcgg tggcgtttgg tggcgatgct cttgacgttc gccaccgcgt
     2521 cgttagtggc tttttccatc atggtagagt cggccttgcc gccctgtagt tccaccgggt
     2581 gggcggcgcc gatgttcgtc cccgaagcca tggcggcgat gtgggccgca taggtaatgt
     2641 acattcccgc cgaagcggcc cgggcgcccg aaggggctac gtagactatc accggcacgt
     2701 cggcgttcaa gatgtctttg gtgatctgcc aggtggagag caccagcccg cccggggtgt
     2761 tgagttgaat caccagcgct tcggcctgct tttcttcggc tttatcgatg gcctgagtga
     2821 ttaacttggc ggtaaccggc gtaatggcgc cttccagtgt cagaaggtac acctcgtgtg
     2881 tgccttcgcc cgggggagat gcttttttct cctgggcctg cgccagaagc aaagaggccg
     2941 ccaccagaca gagtaccgta gaaagagatc tcttgatttt cattgctggc atcctcccat
     3001 tccgtgcgaa ctatacgtaa tcgctccgaa atggatagac tgacagaaga gaaagtaacg
     3061 ccaaaagcag ggcgtgtcaa ttatacccgc ctgccggact tcttttgctt acgctgaaag
     3121 aaatcaatat gttttgccga ggatacaggc cgaccggctc gctgtcgatg gcataataga
     3181 tgaactctca attgaaaaag cagtcatccc agctcatggt gagtgtttcc ggcgtgcgcg
     3241 gggtagtcgg ggaaaccctg actcccgaag ttattgtccg gtacggtttg gcctttggtc
     3301 aatttgtaaa gcgaaagaaa gtcgtaatcg gccgggatgc gcggccctca ggtccgatgg
     3361 tgcagaactt agccgccggc gcgctccttt ccacgggctg cgatgtggtg gacatcggtt
     3421 tgaccaccac tccgacgttg gagttggccg tcacagattt gaaggcttcc ggcggggtga
     3481 tggtcaccgc cagccacaat ccggcggaat ggaatgccct caagtttttc aatgcttcgg
     3541 gcacgttctt gacaccccag cagacaaaag gagtttttga tcgagcgaag aagatgcatc
     3601 ccggcgtcaa gtggaactgg gcaggcaaac tctcgtttga tgcctcccgg aatgccaagc
     3661 acattgaaca aattctcggc ctgccgctca tcaatgcctt tgccatccag aagcggcgct
     3721 ttcgggtggt agttgactgt gtcaatggtt caggcggagt tgtagtcccc gaacttctgg
     3781 cacgattagg atgtgaggtg acccctcttt tctgcgagcc gaccggtctt ttcccccata
     3841 acccggagcc ggtgccaaaa catttgaccg ctttggctca aaaagtcaaa gagaccgggg
     3901 ccgatttagg ttttgccgtt gatccggacg ttgaccgcct ggcgctggtg accgaaaaag
     3961 gggacgccat aagcgaagag tacaccctgg cgctggcggc ggatttggtg ctctccaaaa
     4021 cgcccggccg ggtggtggtc aacctctcca cctcccgaat ggtggaagac gtggcaaaaa
     4081 agcacggaat gccctgctac cggacgccgg tgggcgaagc caatgtcgtc ctcgggctga
     4141 aaaaaaataa aggcgtggtc gggggcgagg ggaacggcgg ggtcatcctg cctgcgctac
     4201 attacggacg ggatgcgtta gtggggatgg cgttgatttt gcaactctta gtcgagcggc
     4261 agaagacctt aaaccagtta gtaagtgagc tacccgcata tcaaatggag aagatcaaaa
     4321 aaccgttgcc cgctggcttt gagaaacggc ttaaaaagct ggctttggcc tttgacggca
     4381 cccggcccga ctggcgggat ggcctccggc tcgacctgcc cgaggggtgg ctgcaggtgc
     4441 ggaagtcgaa taccgagccg gtctgtcgca tcgttgcgga aggtcaatca gaaaagtcgg
     4501 tgaatagaat tctaaaagag gcacaaaaag tcttgcaatc ttaatgagtg agagattatt
     4561 ttaatgttgg caactgatta gggtttgctc ggattatctc cggcaagcag gttagagcta
     4621 accatgtgcg gcatagtcgg ttacgtaggt gaacagaaag ccgttccgct ccttttagag
     4681 ggcctgaaac ggctggagta ccgggggtac gattcggccg ggatagccat cctggaaaat
     4741 ggtgaacttt tttccacgaa agcggcaggc aaaatcgccg ttctcgaaaa agtcctgaat
     4801 ggacgcgaat ttgccgccac ctccggcatc gcccataccc gctgggctac ccacggcgag
     4861 ccgaccgacc tcaacgccca cccgcacgtg gattgcaaag acgagatcgc cgttatccac
     4921 aacgggatca tcgagaactt tgcggcactc aaagctgtcc tcgaacgcca ggggcacaag
     4981 ttcacctccg agaccgatac tgaagtctta gcgcacttaa ttgaagagca ctacgaaggg
     5041 aatctgctcg aggccacccg ggcggccctc tcgctcgttg agggcaccta
//
LOCUS       DBNGZD010000033         4779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_352207, whole genome shotgun sequence.
ACCESSION   DBNGZD010000033 DBNGZD010000000
VERSION     DBNGZD010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_352207"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2170
                     /locus_tag="AC16GM_00990"
     CDS             <1..2170
                     /locus_tag="AC16GM_00990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR2922286.1"
                     /translation="SDYILPVNPAVPQVTHSPAHSVDSAGELFSAKGILAQKTKDFEF
                     RPEQQKMAEAVAEALDSGKILVVEAGTGTGKTWAYLVPVLLWAQENKKRVVISTYTKN
                     LQHQLLSHDLPVIEKALGIDVPVELFKGKENYLCLLRLNRHADETTLFKEKGKFSGVF
                     SWAEKTKTGDLSEYGTNGEPLSTNLRNEVNVENHFCLHQECLYYSPCFYFKARRRAQE
                     AAVVIVNHFLFFADLVTDHSLLGHYDAVVFDEAHRVEEVASRYFGAESDYWTIRRTCE
                     RLYNERPNPSGLLTIISTRINAVLHAKADKNWWQKNLSLAQEKVQGVAQAAFDFFTEA
                     AREVPRENADDSFPIKRRLKSDDDFLRALRHPAEGLGSTLSGLAQQLGKLSNSMQEID
                     PEVLPEVKEYAFELDTRAKELTLTRNTLLELLSAEEDDQVFWVELLPKNPLSVAFHRV
                     PLDIKEELKAKIYDQVEAAVFSSATLAVNGDFGYTCERLGLAALKSGRVNCKQFGSSF
                     PLADQMKVFVADFVGSPLEGEYLNAISNLLSLSCRKVPAKTLALFTSHSHLERVHRSL
                     RAELDEAGVDLFGQVIDGTAEEVLERFRKARRALLLGVAALWEGIDLPGRQLELIYIC
                     RLPFAVPTDPVAQARMEKLEQEGLDPFTNYSLPEAVLRFKQGLGRLIRTRNDRGAVVV
                     LDNRILRKGYGRFFAQALPVELEAVKTEGELFERLAGWLR"
     gene            2183..3067
                     /locus_tag="AC16GM_00995"
     CDS             2183..3067
                     /locus_tag="AC16GM_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR2922287.1"
                     /translation="MKKLIGAAVLVAILITVIVVVKGRQNAIDATRIEASGTIETTEI
                     DVASKTSGRVAVLNITEGDPVKKGDLLLELEHNELDAQHKVAVATIAAAKSRVSQAEV
                     NLANLKDNFARIEALLQSGAATQQVYDDMKAKVTLAEREQEAARASLAQAQAQKELIE
                     TQLENSRIISPATGYVLSKNVEPGEVILPGAPVATIGELTSVWLKIYVKETQLGHIKL
                     GQPVKVRIDSFPKRDFEGKITYISNEAEFTPKSIQTKDERVKLVYAVKVTIPNPNLEL
                     KPGMPADAVIEVGSSDDK"
     gene            3074..4600
                     /locus_tag="AC16GM_01000"
     CDS             3074..4600
                     /locus_tag="AC16GM_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2922288.1"
                     /translation="MGYYFGEMTRLCRFLKRCHLREVFLVLIFPFSSASGQMLYQKSG
                     AAVNDLFGYSVAGAGDVDGDRRADFIVGALYASPGGLYAAGSAYVYSGATGDLLYQLN
                     GSSSGAWFGRSMGNAGDVDGDGKDDFIVGATGSASIQAGSAYVYSGATGTLIYQINGT
                     AGDMLGLSVAGVGDVDGDGKADFIIGAPNAEPGGFSSSGSAYVYSGATGGLLYQFNGI
                     ADSMFFGYPAVGVGDVDADGRPDFIVGAIGASPSGLTDAGSVYVYSGVNGSLIYQKDG
                     SAPGDQFGYSVDGVGDLDGDSKSDFMVGAPFADPNEVNLAGSVFVYSGATGGLLFQKN
                     GAAVDDHLGWRVAGSGDIEGDGKPDFMIGAPLAHSGGFRWAGSAFIYSGATGDLLFRK
                     VGSAGDYMGNSVAGTGDVNGDGRSDFIIGAPFADPGGLTNSGSAFVMTCAGLCGDVDN
                     DSSTTIADIIALINNVVFETGVANPTAGDTNCDEVRNIEDIVLLIQYVVFGTPTPCCL
                     "
BASE COUNT         1037 a         1209 c         1416 g         1117 t
ORIGIN      
        1 ttctgactat attctccccg tgaatcccgc ggtgccacag gtcactcact cgccggctca
       61 ctccgttgac agcgccggcg aacttttcag cgctaaggga attctcgccc agaaaaccaa
      121 agatttcgaa tttcgtcccg aacagcagaa gatggccgaa gcggtggcgg aggcgctcga
      181 ctccggaaaa attttagtcg tcgaagccgg aaccggcacg ggcaaaacct gggcatactt
      241 ggttccggtt ctgctctggg cgcaggagaa caaaaagcgc gtcgtcatct ccacctacac
      301 caaaaacctc cagcatcagt tactctccca cgatctgccg gtgatcgaaa aggcgctggg
      361 aatcgatgtg ccggtggagc ttttcaaggg caaggaaaac tatctttgcc tgctgcggct
      421 gaatcggcat gcggacgaaa ccacgctctt caaggaaaag ggcaaattct ccggagtttt
      481 ctcgtgggcg gaaaagacca agaccggcga tctctcggag tacggtacta acggcgagcc
      541 gctttcgacc aatctccgca atgaggtgaa tgtcgaaaac cacttttgcc tgcaccagga
      601 gtgtctgtac tactcgccct gcttctactt caaggcccgg cgccgggcgc aagaggcggc
      661 ggtcgtcatc gtcaaccact tcctcttctt tgccgatctg gtgaccgacc actcgctttt
      721 gggccactac gatgcggtgg tttttgacga agcccaccgg gtggaagaag tggccagccg
      781 ctacttcggc gcggagagcg actactggac catccgccgc acttgcgagc gtttatataa
      841 tgagcggccc aatccttcgg gtctcttgac gattatctcc acgcgcatca atgccgtcct
      901 ccacgccaag gcagacaaaa actggtggca gaaaaatctc tcattggcac aggagaaagt
      961 acaaggggtc gctcaggcag cgttcgattt ctttaccgag gcggctcgtg aagtgccgag
     1021 agagaatgcc gatgattcct ttccgatcaa gagacgcttg aagagtgatg atgatttttt
     1081 gcgggcgctc aggcacccgg cggaaggtct cggctccaca ctttccggtc tggcccagca
     1141 attggggaag ttatcaaatt cgatgcagga gattgacccc gaggttttgc ccgaagtcaa
     1201 ggagtatgcc tttgaattag acacacgcgc caaagaacta accttgacgc gcaacactct
     1261 tttggaactc ctctccgcag aggaagacga tcaggtcttc tgggtggaac ttttgcccaa
     1321 aaatccgctc tcggttgcgt ttcaccgggt gccgctggac atcaaagagg aattgaaagc
     1381 gaaaatctat gaccaggtcg aagcggcggt gttctcgtcc gcgactctgg cggtcaatgg
     1441 cgatttcggc tacacctgcg agcggttagg gcttgcggcg ctcaagagcg gaagggtaaa
     1501 ctgcaaacag ttcggctcct cttttccact cgctgatcaa atgaaagttt tcgtcgccga
     1561 tttcgtcggg tcaccgctcg aaggagagta tctcaacgca atttcaaatc tgctctcatt
     1621 gtcctgccgg aaagtgccag ccaagacgct ggcgcttttt acatcacaca gccatttaga
     1681 gcgggtgcac cgaagcctgc gagccgaact cgacgaagca ggtgttgatc tgttcgggca
     1741 ggtcatcgac ggcacagcgg aggaagtctt agagcgtttt cggaaagcgc gtcgcgcgtt
     1801 gctcctgggc gtggcggcgt tgtgggaggg gattgacctg ccggggcggc aattagaact
     1861 catctacatc tgccgcctgc cgttcgccgt gccgaccgat ccggtcgctc aggccagaat
     1921 ggaaaagtta gaacaggagg gacttgatcc gtttaccaac tactctctgc ccgaggccgt
     1981 cttacggttc aagcaaggct tgggccggtt gattcgaaca cgcaatgacc gtggtgcggt
     2041 cgtggtgctg gataatcgta tattgcggaa agggtatggc cggttttttg cgcaagcgtt
     2101 gccggtggaa ttggaagcgg tcaaaactga gggagaactt tttgaacggc tcgcgggctg
     2161 gctcaggtag gagagaagat aggtgaaaaa gctcataggt gcggcggtgt tggttgccat
     2221 cctgatcacc gtcatcgtcg tggtcaaggg ccggcaaaat gccatcgatg ccacccgcat
     2281 tgaggcctcc ggcaccattg agaccaccga aatcgacgtg gcctccaaaa cgtccgggcg
     2341 cgtggccgtc cttaacatca ccgaaggtga tccggtgaaa aaaggcgacc tgctcttgga
     2401 attagagcac aatgaattgg atgcccagca caaagtggcg gtagcgacga ttgcggcggc
     2461 gaagtcgcgc gtgagccagg cggaggtgaa tctggcgaac ctcaaggaca actttgcgcg
     2521 gattgaggcg ctcctgcaat ctggtgcggc gacccagcag gtttacgatg atatgaaagc
     2581 gaaggtaacc ttagccgagc gcgaacagga agccgcgcgc gcttctctcg ctcaggcgca
     2641 agcgcagaaa gagttgatcg aaactcaatt agagaacagc cggataatct cccccgccac
     2701 cggctacgtc ctctccaaga acgtcgaacc gggcgaggtg atcctccccg gcgcccctgt
     2761 ggcgaccatc ggtgaactca cctcggtatg gctgaaaatt tacgtgaagg aaacgcagtt
     2821 aggccacatc aaattgggcc agccggtgaa agtgcggatt gattccttcc ccaaacggga
     2881 ctttgagggc aaaatcacct atatttccaa cgaagctgag tttacgccca agagcattca
     2941 gaccaaagac gagcgggtga aattggtcta cgcggtgaaa gtgacgattc ccaatcccaa
     3001 cctggaactc aagcccggca tgccggcaga tgcggtgatt gaggtgggga gttcggacga
     3061 taagtgatat ggaatgggct actactttgg cgagatgacc cgcctttgcc gtttccttaa
     3121 gaggtgtcat ttaagggaag ttttcctggt tctaatcttt ccattttcat ccgcttctgg
     3181 acaaatgctc taccagaaaa gcggtgccgc cgttaatgat ttattcggtt attcggtagc
     3241 tggagccggt gatgtggatg gtgaccggag agccgatttc atcgtagggg ctctttatgc
     3301 aagtccgggt ggtctttatg ccgcagggtc agcatatgtc tactccggtg ccaccgggga
     3361 tctactttac caactaaatg ggtcttcgtc cggggcttgg ttcggacgtt caatgggaaa
     3421 tgccggggat gttgacggag acggaaagga cgactttatc gtaggggcta caggtagtgc
     3481 ttcgatacag gccgggtcgg cttacgtcta ttctggggcc acaggcacgc taatttatca
     3541 gataaacggt actgccggtg atatgctggg gttatctgtg gccggggtcg gagatgtgga
     3601 tggcgacggc aaggccgatt ttatcatcgg cgctcccaat gctgagccgg gcgggttttc
     3661 ctcgtctggt tcggcatatg tctactccgg tgctactgga gggctgcttt accaatttaa
     3721 cgggatcgct gacagtatgt ttttcggtta tcctgcggtc ggggttggtg acgtagatgc
     3781 cgatggacga cctgatttca tcgtcggcgc tattggcgcc agcccaagtg gtctaactga
     3841 tgccggttcg gtttacgtgt attccggggt gaacggttcc ctgatttacc agaaagatgg
     3901 tagtgccccc ggggaccagt ttggctattc tgtggatggt gttggagatt tggatggcga
     3961 tagcaaaagc gattttatgg ttggcgctcc ttttgccgat ccgaacgaag tgaatttggc
     4021 tggatcggta tttgtctatt ctggagccac tggtggtttg cttttccaaa aaaatggtgc
     4081 tgcggtcgat gatcatttgg ggtggagagt ggcgggatcc ggtgacatag aaggcgacgg
     4141 caagcccgac tttatgatcg gagcgccctt agcccactct ggtggttttc gctgggctgg
     4201 ttctgctttt atctactcgg gggcgactgg cgaccttctc tttcgaaaag tcgggtctgc
     4261 tggcgactat atgggtaatt cagtggcggg gaccggagac gtgaatgggg atggacgatc
     4321 agatttcatt attggtgctc ccttcgcaga cccgggtgga ctaactaact ccggttcggc
     4381 ctttgtcatg acctgcgctg gcctgtgcgg ggatgtggac aatgacagtt caactaccat
     4441 agcggacatc attgctctca tcaataatgt ggtgtttgag accggagtgg ctaaccccac
     4501 agcaggcgac accaattgcg atgaagtgcg gaatatcgag gacattgtgc tgctcattca
     4561 gtatgtggtg tttggtacgc ccacaccctg ttgtttgtaa aggttgggtg tgtggtgagt
     4621 ccgcctgagg cggatcgaac cataacccca ctccctgttg tttgtaatta ttgttgaccc
     4681 ctccccgcct gaggcgggtc ttcggcctct ctcccttcga agaggaagga attttcttgt
     4741 ctccctctcc tgtaggagag ggttggggtg aggtcacct
//
LOCUS       DBNGZD010000034        11270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1672376, whole genome shotgun sequence.
ACCESSION   DBNGZD010000034 DBNGZD010000000
VERSION     DBNGZD010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1672376"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="AC16GM_01005"
     CDS             <1..1758
                     /locus_tag="AC16GM_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR2922289.1"
                     /translation="RGSELRGLKSFLEKSRAGIIRQFQTNFSHEESALLAGFLLGYTR
                     DIPSEIYRLFRDTGTLHLLAVSGANVGLVVGLVWFLARLFLFPRRLTVVLVLAAIWFY
                     AGLALFQPSVIRAAIMWSLGALAFWFYRTPKAVNIIGVAALGMLGFKPLWLFDVGFQL
                     SFAATLGIVVFVPQVAEWVNRLPGYIRLPARFSLLPLAVSLIAQLATWPFIVYYFHRV
                     SIVSPLANLCIVPLASLAMYSTIPFLAAAKIFPPLGHLVAGAAAKILGLIFFFEERFG
                     ADRAAAHVLGGCDLLCFAALGVLAGSLLALTFFPHRRWLSAALVLGMLNFLAWRSVLH
                     ESPSVRATFLEASPGRWLVVQAGSTAALFGSGGRSQIRYVVVPFLAQAGIQRVTACFP
                     FDGGDSTRRALEELCREVTVNRFVWPQAAGERRADFPERAVAGRSGDSLAIVGIVAQP
                     APMLSGYLCRTRYFSILSVVRADWVTQTDFWNRLAVFAPTVVSLPIQAASPEVLDALA
                     RQAPATVVLVEGRQRFGWEREQMWRGATGSQGRLPQHLMILSSCGAVTLAATEKRLRA
                     ESYLGGRFLSRTVPAVHNR"
     gene            1868..3058
                     /locus_tag="AC16GM_01010"
     CDS             1868..3058
                     /locus_tag="AC16GM_01010"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR2922290.1"
                     /translation="MSFAGNLATVTFADILQLLSTGKKSGALVITRGSQSKEIFFRNG
                     HVIYATSTNADEDLLGQLLVKRGTIKQADLDRALALQKTSGKKLGATLIELQLISQQE
                     MAACLKIQIEEIVYSLFGWNQGEFNFHEGRTPSANTLVTELNTMNIVMEGMRRIDEWG
                     QIQKNLPPDNIPLKVTMPPPVKGEVPLSADEISLLTLVNGQRTLSDLIRESPVGEFAT
                     TKAIHRLVSAGLLEKSNAPRLLVSEEKNEEHLWTLMLKVYASAFAVVARTLQQKLGEA
                     GQHLYRNVPEKTARGMDAQLVQFVFGEGFDHKALFAALAGVPREIRLFSLCNRFNRLL
                     AEALTVVQCALGGAVKKQVVAEIKREIGLVLGESRSLATAFQLEGELTSTLRQLAGRD
                     GYRG"
     gene            3069..3446
                     /locus_tag="AC16GM_01015"
     CDS             3069..3446
                     /locus_tag="AC16GM_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922291.1"
                     /translation="MVRGLASFIAICVILVAAVPVAAEVTAAPSRSFLGAIFELVSAG
                     VAALCLLACFKLSTSTKGGEVASSWQVAAGSFLLFTLGELVAFLSQIGVLPPVESWVA
                     LFRVAAIGAFFWALTRMRKVFST"
     gene            3461..4324
                     /locus_tag="AC16GM_01020"
     CDS             3461..4324
                     /locus_tag="AC16GM_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922292.1"
                     /translation="MLNLAEIDERIQKCEKILSENPGSQIFAALADSYRRRGLLDRAF
                     AICREGLRRHPNYGPGHLVMAKLHLERRMFELAEREARLAMELEGRTRVTEILLAEIY
                     LGHGRFADALALLEPLAATGPRDPQIKTLITKAKIGAQKSRTVPVRTKAPAAEVEPEP
                     RPVPLDPAGALQLLRDFAGVAGAAIVSREGLVLEQVGLPKNLAEAVGAVASAIFGSTA
                     ASLPEPVFGSCEQLWIETDAARYWIAAVVEKIWVVVCRPDINFGSFKLHAEEIKRRVR
                     DDAPQPTPLEV"
     gene            4327..5310
                     /locus_tag="AC16GM_01025"
     CDS             4327..5310
                     /locus_tag="AC16GM_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922293.1"
                     /translation="MARNRREGRLVWVWSVVLALVPLVLYPNSLGTAGWLLGPQRLLF
                     EAVFYLVAFSFLFAEAGLQRLLRPLFVSWIFRGAIGLLFGLAAAVVHGTAVIEALLDG
                     WYRYLPALFLQALATPFVLRGWVGRWLVEARAAARLATFEPAPGPLPVPATGVPHHRT
                     PRPQPYLAASVEPVAASVQKVGREVTWEDAVRFVGEHAGVIKCFLVDEEGLTIASWFR
                     DGAVEDDLWAGLSLRFWEANAGAIARAGARQVTKLEVHTEQEKVFLNRIDLFTLVAVA
                     EPDADELVNVRLARATEMIAAYWHDRAEVLKSHLKTTGQTEEHHVSRVGVT"
     gene            5285..5623
                     /locus_tag="AC16GM_01030"
     CDS             5285..5623
                     /locus_tag="AC16GM_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR2922294.1"
                     /translation="MYHVLESLNKTTGITGSMLVGNDGIVIAADLTEQIDSETIGALA
                     ASITANVQKSLDRLQQTPLRQITIEAQKGKLFLTDVGRGVLVVTTEPQVNIGLVRLEI
                     KEAAQKILRA"
     gene            5631..6209
                     /locus_tag="AC16GM_01035"
                     /pseudo
     CDS             5631..6209
                     /locus_tag="AC16GM_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            6691..7173
                     /locus_tag="AC16GM_01040"
     CDS             6691..7173
                     /locus_tag="AC16GM_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR2922295.1"
                     /translation="MANEALVLYEEQMALIDRTLAKLLKAAEAKCALLVDKDGHLVTR
                     QGFTHSLDTTALAALLAGSFASTREIARLVGEPEFSVLFHQGKRDHIHINLVGERSIL
                     VVIFDDRTTIGMVRLYAKEISDELVKILSAKKKGHKGPAITADFAASAESRLDDVFQD
                     "
     gene            7252..8949
                     /gene="pilB"
                     /locus_tag="AC16GM_01045"
     CDS             7252..8949
                     /gene="pilB"
                     /locus_tag="AC16GM_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HIR2922296.1"
                     /translation="MSAELGSMLVKAGKITEAQLTRALEIQKNSKEKLATILVKIGAL
                     ADEDDVSEFIGRQLNMGTLRLADIELNPDVVKLIPLDIARKFNVIAISKLGKTLVVAI
                     SDPNNIYVLDAIKFITGCAIQPVISPEKAIQKAIEHYYQDTGNLKEILGQIEGEGLEI
                     VEAGEGEAPSEQDLQSAVQDKPLVKLVDHLIADAIRKGASDIHLESYEKRIRVRYRID
                     GTLREMAPLPFKYRAAIVSRVKIMADLDISERRLPQDGRIKVKITERTVDLRVSVLPT
                     IFGEKVVMRILDPKSLMVDLTRLGFHEHSLGHFQKAIELPYGIILVTGPTGSGKTTTL
                     YSALKQINTNDINIMTAEDPVEFNFDGVNQVLVRSDIGLTFSAALRSFLRQDPDVIMV
                     GEMRDQETAEIAIRAALTGHLVFSTLHTNDAPSTVNRLVDMGIPAYLVASATKLIMAQ
                     RMVRKICSVCKADDPDVSPQALERLGVPPNEIKAFKAYKGKGCNECGGTGYSGRSGLF
                     EVMPVTPAIEHLILNNATASDIRKVAVEEGMLTLRMAGIEKLKQGLTSLEEVLVETA"
     gene            complement(9046..9513)
                     /locus_tag="AC16GM_01050"
     CDS             complement(9046..9513)
                     /locus_tag="AC16GM_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922297.1"
                     /translation="MRKIVVVFLTIVIWTGWLIASGQAQMSSTNFRIPTQALSSSGGA
                     GNSTNFKQPVSIGGQSTPIGTATSTNFDNFAGYLYTLGGGACALLGDLTGDLVVDIVD
                     IVAMVNNVVFGDPIPTGAICADIAPIPLDGVIDITDIICLINNVVFSNPLPCP"
     gene            9968..>11270
                     /locus_tag="AC16GM_01055"
     CDS             9968..>11270
                     /locus_tag="AC16GM_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2922298.1"
                     /translation="MRRFARISLAPLGFCLVFSVFSVSVWAGVTGKIAGVTRDKETKK
                     PIPAATVQLVGTAVGAFSNPDGEYFLINIPVGTYKIKATSVGYAPVEVDNVHVTADQT
                     TTLDLELEPQAVQMQEIRVTAERPLIQRDQTGTSRVVTSQEFANLPVRDFMDAAALQT
                     GVVKGLISPPYLDRSRGDDISSGLYVRGGRSNEIGTYVDGFTQQNPLTGLSTVTINPN
                     AIEEIQVLTGGFSAEYGRIMSGVVNTVTRSGTAKYHGSFGAVTDNLAGRWIDTKSFDN
                     NVYEASFGGPFKPNDDRYTFYFSGEQRWNRDQSPGRVSSVAQQTIDDIKRTQGFSEGD
                     IRSEQGRLPANHLKGWSAQGKLSFKLTPNMNLQAGALLTQDDFRQYRQEFLFDLDHTP
                     RVINTNRSVFVKLNHNLNRKTYYNAAVNWFFTDRFRGDGLYG"
BASE COUNT         2249 a         3211 c         3452 g         2358 t
ORIGIN      
        1 cgaggctctg agctgcgtgg actgaaatca tttttggaaa aaagccgggc cgggatcatc
       61 cggcagttcc aaaccaattt ttctcatgag gaaagcgcgc ttctggccgg gtttctcttg
      121 ggctacaccc gagacatacc gtcggaaatc taccgtctct tccgcgatac cggtacgctg
      181 catctgctgg cagtcagcgg ggccaatgtc ggcttggtcg tggggctggt ctggtttttg
      241 gcgcgtctct ttcttttccc ccgccgcttg accgtggtcc tggtgctcgc ggcaatctgg
      301 ttttatgccg ggctggcgct cttccaacct tcggtgattc gcgcggcgat catgtggagt
      361 ctgggggcgc tggccttctg gttttaccga acacccaagg cagtcaatat catcggggtc
      421 gccgccttag ggatgctcgg ttttaagccc ctctggctgt ttgacgtggg atttcaactt
      481 tcttttgcgg ctactttggg aatcgtggta ttcgttccgc aggttgccga atgggtgaat
      541 cgcttgcccg gatatattcg cctgcccgcg cgattttcgc ttctccctct cgcggtctcg
      601 ctcatcgcgc aactggcgac ctggcccttc atcgtctact atttccaccg cgtatcaatc
      661 gtctcgcctt tggcgaatct ctgcattgtg cccctggcaa gtctggcgat gtactcgact
      721 atcccttttc tagcagccgc gaagattttc ccgccgctgg gtcacctggt ggcgggagcg
      781 gcggccaaaa ttctcggttt gatttttttc tttgaagaac gattcggtgc cgaccgggct
      841 gcggcgcacg ttttgggtgg gtgtgacctc ctgtgtttcg cagcgctcgg ggtgcttgcg
      901 ggatcgctat tggctctgac cttcttcccg caccggcgct ggctgtccgc ggcgctggtg
      961 ctggggatgc tcaatttttt ggcctggcgt tcggtgcttc atgaatcgcc gtcggttcga
     1021 gcaacctttc tggaggccag cccgggacgc tggctggtcg ttcaggccgg ctccaccgcg
     1081 gcgctcttcg gcagcggcgg caggagccaa atccgctacg tcgtcgttcc ctttctggca
     1141 caggcgggta tccagcgggt taccgcctgc ttcccttttg atgggggcga ttccacccga
     1201 cgggcgctgg aggaactgtg tcgggaagta acggttaacc gttttgtctg gccgcaggca
     1261 gcgggagaga gacgagccga ttttccggag agggccgtcg ccggccgttc cggagattct
     1321 ctggccatcg tggggattgt cgctcagccg gcgccaatgc tctccggtta cctttgtcgg
     1381 accagatact tttccattct gagtgttgtg cgcgcggact gggtcaccca gacagatttc
     1441 tggaatcgtc tggctgtttt tgcgcccaca gtggtgtccc tgccgattca ggccgcttcc
     1501 ccggaggtgc tggacgcact ggcccggcag gcgccggcaa cggtcgtgct cgtggaaggc
     1561 cgacagcggt tcggttggga gcgtgagcag atgtggcggg gggcgaccgg cagtcagggg
     1621 cgcctacccc agcaccttat gattttatct tcctgcggcg cggtgacttt ggcggcaacc
     1681 gaaaagcgcc tccgggccga aagttacctc ggtggtcgat tcttgagcag aaccgtgccc
     1741 gccgtgcaca accgttaaga aaacctttct ctatcccgat acataaacag aagatggctc
     1801 tgctacgggc ggagtgtgtc ggcgtttgtg ccgccgttcc ccgcgcgggg cgtacgagga
     1861 tggtgcgatg agttttgcgg gcaatctcgc aacggtaacg ttcgccgata ttttgcagct
     1921 gctctccacc ggcaagaagt ccggtgcctt ggtcatcacc agggggagcc agagcaaaga
     1981 aatttttttt cgcaacgggc acgttatcta cgctacatcg accaacgctg atgaagatct
     2041 tttgggtcag ttgttagtca agcggggcac catcaagcag gccgatctgg accgggccct
     2101 ggcgttgcag aaaacttccg ggaagaaact gggcgccacc ctgatcgagc tgcaactgat
     2161 cagtcagcag gagatggccg cctgcctgaa aattcagatt gaagaaatcg tgtacagtct
     2221 gttcggctgg aaccaggggg agttcaattt ccacgaaggc cggaccccct ccgccaatac
     2281 gctggtcacc gagctgaaca ccatgaacat cgtgatggag gggatgcgcc ggatcgacga
     2341 gtgggggcag atccagaaga acctgccacc ggataacatt ccgctgaagg tgaccatgcc
     2401 gcccccggtg aaaggcgaag tgcccttgtc ggctgacgaa atttccctgt taactctggt
     2461 caacggacag cggacgttgt cggacttaat ccgcgaaagt ccggtgggag aattcgccac
     2521 caccaaagcg atccaccggc tggtgtcggc cggccttctg gaaaagagca acgcgccgcg
     2581 gcttttggtt tccgaggaaa agaacgagga gcacctctgg acgctcatgc tcaaagtcta
     2641 cgccagcgcg tttgcggtcg tggcccgcac cctgcagcaa aagttggggg aagcgggtca
     2701 gcacctgtac cggaatgttc cggaaaagac cgcgcgcggc atggatgcgc aactcgtgca
     2761 gttcgtcttt ggtgaaggtt ttgaccacaa ggccctgttc gcggccctgg ctggcgtgcc
     2821 ccgggaaatt cggctcttca gtctctgcaa ccgtttcaac cggcttttgg ccgaggccct
     2881 gacggtggta cagtgtgcgc tggggggcgc cgtgaaaaaa caggtggtgg cagaaatcaa
     2941 acgggagatc gggttggttc tgggcgagtc gcgaagtctg gcaaccgcct tccagctgga
     3001 aggagaactg accagcacgc tgcgccaact ggccggtcgc gacgggtatc ggggatgagg
     3061 aggaacatat ggtacgaggc ctggcgagtt tcatagcgat atgcgtcata ctggtggccg
     3121 cagtgccggt cgcggccgag gttaccgcgg ctccgtcccg gtcgttcctc ggtgcgattt
     3181 tcgagttggt ttccgccggc gtcgcggcgc tgtgcctttt ggcctgtttt aaactgtcca
     3241 cctccaccaa agggggtgag gtggcctcca gctggcaagt tgccgcaggt tcattcctcc
     3301 ttttcactct tggcgaactc gtggctttct taagccagat tggtgtgctc ccgccggtgg
     3361 agagctgggt cgccctgttc cgggtggccg caatcggagc gttcttctgg gccctgaccc
     3421 ggatgcggaa ggttttcagt acctgaagcg tgagtgccgg atgctgaatt tagccgaaat
     3481 cgacgaacgc attcagaagt gcgagaaaat cttaagcgag aatcccggct cgcagatctt
     3541 cgccgcgctg gctgattctt accgtcggcg cgggctctta gaccgggcct ttgccatctg
     3601 ccgggagggc ttgcgccggc atccgaacta tggtcccgga cacctggtga tggccaagtt
     3661 gcatctggag cggcgcatgt tcgaattggc cgaacgcgag gcccggctgg ccatggaact
     3721 ggaagggcgg acccgggtga ctgaaatcct tttggcggaa atttacctgg ggcacggccg
     3781 ctttgccgac gcgctggcgc tcctggagcc tttggcggcg accggcccgc gcgatcctca
     3841 gatcaaaacg ctcatcacga aagcgaaaat aggcgctcag aaatcccgca cggtgcccgt
     3901 caggacgaag gcgccggccg cggaagtcga gccggagcct cgtccggtac cgctcgaccc
     3961 tgcgggagcc ttgcagctcc ttcgcgactt tgcgggcgtg gccggggcgg cgatcgtttc
     4021 gcgggaaggc ctggtgctcg aacaggtcgg tttgccgaaa aacttagccg aggccgtggg
     4081 ggcggtcgcc agcgccatct tcggttccac ggcggccagc cttccggagc cggtcttcgg
     4141 gagctgtgag cagctgtgga ttgaaaccga cgcggcgcgc tactggattg ctgcggtggt
     4201 cgaaaaaatt tgggtcgtcg tctgccggcc cgacattaac ttcggatcgt tcaaactgca
     4261 cgccgaagaa atcaaacgac gggtgaggga cgacgcaccc caacccacgc cgctcgaggt
     4321 gtaacgatgg cacgtaaccg cagagaaggt cgtctggttt gggtctggag tgtggtgttg
     4381 gcactggtgc cgctcgtgct gtacccgaat tccctgggga cggcggggtg gcttttggga
     4441 ccgcagcggc tgctcttcga agcggtgttc tacctggtcg ctttctcgtt tctgtttgcc
     4501 gaggctgggt tgcaacggct cctccgtccg ctcttcgtca gctggatttt ccgcggcgct
     4561 atcggtcttt tgttcggttt ggccgccgcc gtggtccacg gcaccgcggt gattgaagcg
     4621 ctcttagacg gttggtatcg ctacctgcca gcgctgtttc tccaggcgct ggctactccc
     4681 tttgtcctca gagggtgggt ggggcgttgg ttagtcgaag cgcgcgcggc tgcccgcctg
     4741 gcaacgtttg aaccggctcc ggggcctctg ccggtaccgg caacaggcgt accccatcac
     4801 cggacacccc gcccgcaacc gtacctcgct gcttcggtcg aaccggtggc ggccagcgtg
     4861 cagaaggtgg gccgggaagt cacctgggaa gatgcggtgc ggttcgtcgg cgaacatgcc
     4921 ggggtcatca agtgttttct ggttgacgag gaaggactga ccatcgccag ttggttccgg
     4981 gatggcgcgg tcgaagacga cctgtgggcc gggttgtccc tccggttctg ggaagcgaat
     5041 gccggggcga ttgcccgtgc cggagcacgg caggtcacca agctcgaagt ccacaccgaa
     5101 caagaaaagg ttttcttgaa tcgtattgat ctcttcaccc tggtagcggt ggcggaaccg
     5161 gacgccgacg agctggtcaa cgtgcggctg gcccgggcca ccgagatgat cgccgcctac
     5221 tggcacgacc gcgcagaagt gctcaaatcc cacctcaaaa cgacgggaca gacggaggaa
     5281 caccatgtat cacgtgttgg agtcacttaa caaaaccacc gggatcaccg gcagtatgct
     5341 ggtgggcaac gacggcatcg tcatcgccgc cgatctcacc gagcaaatcg acagcgaaac
     5401 catcggcgcc ttggccgcct cgattacggc caacgtgcag aagtccttgg accgcttgca
     5461 gcagaccccc ctgcggcaga ttaccatcga agcgcagaag ggaaaactct ttttgaccga
     5521 tgtgggtcgg ggcgtcttgg tggtaacgac cgaaccgcag gtgaacatcg gtctggtacg
     5581 gctggagatt aaggaagcgg cgcagaaaat cctccgggcg taaccttccc atggtctcga
     5641 tcaattacgc cttccgggag attcactgca agatcgtctt ctacggtccg gggttgtcgg
     5701 gcaagaccac gaacttgcag tacatccata agaaggtgcc gccgaatacg aaaggggagt
     5761 tgatttcttt ggccaccgat gcggaccgaa ccctctactt cgatttcctg ccgatcaaca
     5821 tcggctcagt ggccgggttc accaccaaat tccagctcta caccgtgccc gggcaggtct
     5881 actacaatgc cacgcgcaag ctggtgctgc ggggcgcgga cgggctcgtc ttcgtggccg
     5941 attcgcaggc cgagaaggcc gatgagaata tcgaaagcct gcggaatttg tatgagaatt
     6001 tatgcgaata tgggtacgaa cctgaagaaa ttcccatggt ttttcagtat aacaagcgcg
     6061 acctccccgg ggctgtccct ttggagcaac tggagcaggc actcaaccct aaaagactcc
     6121 cctcgtttga agccgtcgcc accaaaggga ttggtgtgtt tgatacactc aaatgcgtca
     6181 ccaagttggt gctcgataaa gcgcgccgcc agacggagaa aaattccgag gtgacccaga
     6241 ccgacatgcc ggcggcggtt gagaagcagg tgacaccggc ggtcccggcg gctgggcccg
     6301 ggagttcggt ccagccacgc acactggaag agccggtggg ggtgggcgca cctgtcgccg
     6361 caaccatgtt caaagcggag tctgtggcgg cccgagcgcc agtgcttccg caccgcccac
     6421 gtccgggcgg cgctccggaa ccggtgattg gacggctcta tccggaacgg ttttcgggaa
     6481 atagttccgg accgattgcg cctgtgcggc ggccagattc ctcggcagat ccggtgggaa
     6541 aaccggtttt gacgccggcg caattggtgg cctcccggcc gcggttgcgg ccactgccac
     6601 cgaagaagaa gaaaaagtcg ttctggtcac gattgttttc gttggggagg tagttcccgg
     6661 ggtcgttgcg atacctgtaa ggaggtcacg atggcaaacg aagcgttagt actctacgag
     6721 gagcagatgg ccttgattga ccgtaccctg gccaagctgc tcaaggcggc cgaggcgaaa
     6781 tgcgcgctcc tggtcgataa ggacgggcac ctggtcaccc ggcagggttt tacccattcg
     6841 ctggatacca cggccctggc ggcgctcttg gccggcagtt tcgcctccac ccgcgaaatc
     6901 gcccgactgg tgggggagcc ggagttctcg gtcttgttcc accaaggcaa acgcgatcac
     6961 atccacatca acctggtggg cgagcggagc atcctggtcg tgatctttga cgaccggacc
     7021 accatcggca tggtgcgtct gtacgccaag gaaatttccg acgaactggt gaagattctt
     7081 tcggccaaga aaaaaggaca caaagggccg gccatcaccg ccgattttgc cgcttcagcc
     7141 gaaagccggc tggacgacgt gttccaggac tgaaccgttt gaccccgtcc aaggcggggt
     7201 agccactgcc cttcgcggca agcatttcac ttcggatgaa acgagtgtcg gatgtcggct
     7261 gaactgggga gcatgctggt caaagcgggg aagattaccg aagcccagct gaccagagcg
     7321 ctcgaaattc agaagaacag caaagagaag ctggccacca ttctggtcaa gattggtgcg
     7381 ttagccgatg aagacgacgt ttcggagttc atcggccgcc agttgaatat gggcacgctg
     7441 cggctggccg acatcgagct caatccggac gtggtgaagc tcattccctt ggacattgcc
     7501 cgcaagttca acgtcatcgc catttccaag ctcggcaaga ccttggtcgt ggccatctct
     7561 gaccccaaca acatctacgt tttagacgcc atcaagttca tcaccggctg tgccatccag
     7621 ccggtgatca gtccggaaaa agcaattcag aaagcgattg agcactacta ccaggatacc
     7681 ggcaacctca aagaaattct gggtcagatc gaaggagaag gtttagagat cgtcgaggcc
     7741 ggggaagggg aagcaccgtc cgaacaagac ctccaatcgg cggtgcagga caaaccgtta
     7801 gtcaagttag tcgaccacct gatcgccgac gccatccgca aaggcgcctc cgatattcac
     7861 ctcgagagtt acgaaaaacg gatccgggtc cgctaccgaa tcgacggcac cctccgcgag
     7921 atggcacctc tgcccttcaa gtaccgggcg gccattgtct cgcgcgtcaa gatcatggcc
     7981 gatttagaca tctccgaacg ccgcctgccc caggacggcc ggattaaggt caagatcacc
     8041 gagcgcaccg ttgacctccg cgtatcggtc ctgcccacca ttttcggcga aaaagtggta
     8101 atgcgtatcc tggaccccaa gagcttgatg gtcgacttga cccgattggg tttccacgaa
     8161 cactccctgg gacatttcca gaaagcgatc gaactcccct acggcatcat cttggtcacc
     8221 ggaccaaccg gttccggaaa aaccaccacc ctttattcgg ccttgaagca gattaatacc
     8281 aatgacatca acatcatgac cgctgaagat ccggtggagt tcaacttcga tggggtcaac
     8341 caggtgctgg tgcggagtga tatcgggctg accttttcgg cagccctacg ctcgtttttg
     8401 cggcaggacc cggacgtcat tatggtgggt gaaatgcgtg accaggaaac cgccgagatc
     8461 gccatccggg cggccctcac cgggcatctg gttttctcca cgctccacac caacgatgca
     8521 ccctcgacgg tgaatcgtct ggttgatatg gggattccgg cttacctggt cgcctcggcg
     8581 accaaattga ttatggccca gcggatggtg cgcaagatct gttcggtctg caaggcggat
     8641 gacccggatg tgtcgcccca agctctggaa cggctggggg taccgccgaa tgagataaaa
     8701 gcgtttaagg cctataaggg aaaagggtgc aacgagtgcg gcggaaccgg ctactccggc
     8761 cggtcgggtc tttttgaagt gatgccggtc acgccggcca ttgaacactt gatactcaat
     8821 aacgccacgg cttccgatat ccgcaaagtc gccgtcgaag aaggaatgct caccctgcgg
     8881 atggccggga tcgagaaact caagcagggc ctcacctctc tcgaagaagt gttagtcgag
     8941 acggcgtagt atttcgactt cacttctccc aaaaaagaaa ggcaccccta cggtcggagc
     9001 gactcgaggc agggtgcttt tctttccgta ccttgatcct ctcccttacg gacagggtaa
     9061 ggggttgcta aagacgacgt tgttgatcag gcagatgatg tcggttatat cgataacgcc
     9121 gtcgagagga atgggggcga tatcggcaca gattgcgcca gtggggatgg gatcgccgaa
     9181 gacgacgttg ttgaccatgg cgacaatgtc cacaatgtcc accaccagat caccggtcaa
     9241 gtcacccaag agggcacagg cacctccacc caaagtgtac aagtagccgg cgaagttgtc
     9301 gaagttggtg ctcgtggcag tgccaatcgg cgtgctctgg ccgccgatgg agaccggctg
     9361 cttgaagttg gtcgaattcc ccgcaccgcc ggaggaactc aaggcctgcg tgggaatcct
     9421 gaagttggtg ctggacatct gggcctggcc gcttgcgatc aaccaaccgg tccagatgac
     9481 gatagtcagg aaaacgacca cgatctttcg catggctgct cctttcagac tccttaccgt
     9541 tttcgcgttc atacgcataa actgtcgtgc gccctccctc gggggataag aaaagggcag
     9601 ggccaactcc tgtcaaggga ttttaaggct cagggcaaga tttttttcaa aatatgcaaa
     9661 ttttacttga caaatgaacc ggtgtactgt tcctatatcc ccggaagtta agaagttgca
     9721 gtgactgtgg gccgtttacg gctcgttttg agaaacgaac caaaaggagg agaatcggtc
     9781 caacgcgaca gagtatagcc tgacaactgc atatctgccg tcgcgcggtg aaggaattgg
     9841 cccacgtgcc gatgttcctc atagatagag gagtatggga gtagtgggat tccaggatgg
     9901 atgtcgtggt acccggccgc caggtcggga gaagtttact ttcctttcca accaaaaggg
     9961 aggagttatg cgcaggttcg cgcggatcag tctggctccg ctggggttct gtctggtgtt
    10021 ttccgttttt tcggtttctg tttgggcggg agtgaccggg aagatcgccg gagtgacgag
    10081 agataaggag acgaaaaagc cgattcccgc ggcgacggtc cagctcgtcg ggaccgcggt
    10141 gggcgccttc tcgaaccccg acggggagta ttttctgatc aacattccgg tggggaccta
    10201 caagatcaag gcgacgtcgg tcgggtatgc ccctgtagaa gtggacaatg ttcacgttac
    10261 cgccgaccag accaccacgc tcgatctgga actggagccg caggccgtgc agatgcagga
    10321 gatccgagtg acggccgaac ggcctttaat ccagcgcgac cagaccggga cttcccgggt
    10381 ggtcacgtcg caagaatttg cgaacctccc cgtgcgcgac tttatggatg ccgccgcgct
    10441 gcagacgggc gtggtgaagg gtctcatcag ccccccctat ctcgatcgga gccgtggcga
    10501 cgatatcagc tcgggtttgt acgtccgcgg cggccgctcg aacgaaatcg gcacttacgt
    10561 ggatggcttc acccagcaga atccacttac cgggctttcg acggtgacga tcaacccgaa
    10621 tgccattgag gaaattcagg ttttgaccgg cggtttttcc gccgagtacg gccggattat
    10681 gtccggggtg gtcaacaccg tgacgcggag tggaacggcc aaataccacg gttctttcgg
    10741 agcggtaacc gacaacttag ctggccgctg gatcgacacc aagagttttg acaataacgt
    10801 ttacgaagct tcattcggcg ggccgttcaa gcccaacgac gaccggtaca ctttctattt
    10861 ctccggggaa caacgctgga accgtgacca gtcgcccgga cgcgtcagca gcgtcgccca
    10921 gcaaacgatt gacgacatca agcggaccca ggggttttcc gaaggcgaca ttcgttccga
    10981 gcagggccgc ctgcccgcca atcacctgaa gggctggagc gcccagggga aactgagttt
    11041 caaactcacc ccgaacatga accttcaggc gggggcactt ctgacccagg acgacttccg
    11101 gcagtatcgg caggaatttc tcttcgacct ggaccacacg ccccgggtca tcaacaccaa
    11161 caggtcggtc ttcgtgaagt tgaaccacaa cctcaaccgc aagacctact acaatgccgc
    11221 cgtcaactgg ttctttaccg acaggttccg cggcgacggc ctgtacggcg
//
LOCUS       DBNGZD010000035         2434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_839051, whole genome shotgun sequence.
ACCESSION   DBNGZD010000035 DBNGZD010000000
VERSION     DBNGZD010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_839051"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="AC16GM_01060"
     CDS             complement(<1..247)
                     /locus_tag="AC16GM_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2922299.1"
                     /translation="MGYYLYILRSFVDGNLYIGQTKNLERRVALHNMGKAKSTRRRGP
                     FDLVYVEEFPTRDAAMKKGKYLKSLEGSVLKKAVVEKY"
     gene            complement(262..335)
                     /locus_tag="AC16GM_01065"
     tRNA            complement(262..335)
                     /locus_tag="AC16GM_01065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Gly,seq:tcc)
     gene            complement(369..450)
                     /locus_tag="AC16GM_01070"
     tRNA            complement(369..450)
                     /locus_tag="AC16GM_01070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(414..416),aa:Tyr,seq:gta)
     gene            complement(463..534)
                     /locus_tag="AC16GM_01075"
     tRNA            complement(463..534)
                     /locus_tag="AC16GM_01075"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(500..502),aa:Thr,seq:tgt)
     gene            complement(618..2156)
                     /locus_tag="AC16GM_01080"
     CDS             complement(618..2156)
                     /locus_tag="AC16GM_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2922300.1"
                     /translation="MIEIQNLTLALPVAKGEKIVALSNVSLSVKDGERVAVMGANGSG
                     KTVLLLAVAGLLESSAGEVTIDGLSTKARSDRAKLRDLMGIVFQDPDSQFVAPTVERE
                     IAFGLEQRGFPSQVIREKVEETLASHGLVELRKRPPVDLSGGEKQRLILGAVSVTQPK
                     YLLLDEPTSFLDFPGKELWRKWISERHEERTIIFATQYAEEALLADRLIVLDSGQIAW
                     EGKPQDFFASSGLAEKFGVPESSAKPDLSGKKVTVHHRPLLTTKEITYTYFSFGQESP
                     PALADVGLSLGEGEVIGLIGRTGSGKSTLAQILCGLLIPHKGELLQDDGSGTNTALSR
                     AVLQRIGSMAFQFPERMFFEETVYDEIAFGLRQKGELEESIAVSVYKSLEEVGLPPQA
                     FSFRSPYTLSFGEKRRVALACILALGTALVILDEPTCGLDRPGTQAVRQIIGNLAEQQ
                     KTVVVISHDITLLASAADRFIALERGHVLFDGTNGAFATQDFAQQYGVGTAPSQAASV
                     TVSQ"
     gene            complement(2214..>2434)
                     /locus_tag="AC16GM_01085"
     CDS             complement(2214..>2434)
                     /locus_tag="AC16GM_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922301.1"
                     /translation="FNDVYVTTVTIVPKRNRTAVIDLTPLARLWAREATGNFGVILVP
                     IPDEGQVYKLLPVTPSLAPEMGINISPR"
BASE COUNT          574 a          695 c          632 g          533 t
ORIGIN      
        1 gatacttttc tacgaccgcc ttcttgagaa cactcccttc caaacttttt agatactttc
       61 cttttttcat cgcagcgtcc cgagttggaa attcttcaac gtacaccaaa tcgaatggcc
      121 ctctgcggcg tgtggacttt gccttgccca tattatgcaa agcgacccgc ctttcaagat
      181 tcttcgtctg cccaatgtat agatttccat ccacaaaact acgcaatatg tacaaatagt
      241 aacccacgac cttagtccaa aagcgggaga cgggactcga acccgcgacc aacagcttgg
      301 aaggctgtga ctctagccaa ctgagttact cccgcgaaaa aactcggaca gtgccacaat
      361 aatataaatg gagaggggag gattcgaacc tccgaaggca ttgccggctg atttacagtc
      421 agctccgttt ggccacttcg gtacctctcc gaataaattc ccagccggcg aagggatttg
      481 aacccctgac cagctgatta caaatcagct gctctacctc tgagctacgc cggcagaaaa
      541 cgaaacgctc aaatgcaaac gttcatttta gcatctggtc ttcaggaggt caacaggcgt
      601 ttggagattt cacgaggcta ctgagacacg gtgaccgatg ccgcctgaga aggtgctgtt
      661 cctacgccgt attgttgagc aaaatcctgg gtagcaaatg cgccgttggt tccatcaaat
      721 agtacgtggc cccgctccag cgcaataaac cgatcggctg ctgaagcgag aagggtgata
      781 tcgtgcgaga tgaccaccac cgttttctgc tgctccgcca gatttccgat aatttgacga
      841 acggcttgag tgccggggcg atccaacccg caggttggtt cgtcgagaat gaccaaagcc
      901 gtacccagag ccagaatgca ggccagcgcc acgcgccgct tctctccgaa actcagggtg
      961 tacggagagc ggaatgagaa cgcctgcgga ggaagtccga cctcttccag cgacttataa
     1021 acgctgacgg cgatgctttc ttccaactcg cctttttgcc gcaggccgaa ggcgatttcg
     1081 tcgtagaccg tttcctcaaa gaacatccgc tccgggaatt ggaaggccat actgcctatc
     1141 cgctgcagta ccgctcttga gagtgctgtg ttggtgcccg acccatcgtc ctgaagcaat
     1201 tcacccttgt gtgggatgag caggccgcag agaatctgcg ccagcgtgga cttgcccgaa
     1261 ccggtgcgac cgattaagcc gataacctcc ccctcaccga gggaaagacc aacgtcggcc
     1321 aatgcgggcg gactctcctg tccgaaagag aagtaggtat aggtaatctc tttcgtggta
     1381 agcaaaggtc tatggtggac ggtcaccttc ttcccagaca aatcgggctt tgccgacgac
     1441 tccggcacgc cgaatttttc cgccagcccg gaggaagcaa aaaaatcctg cggcttacct
     1501 tcccacgcga tctggccgga atcaagaacg attaagcgat cggccaaaag cgcctcttca
     1561 gcatattgcg tggcgaagat gattgttcgc tcttcgtgac gctccgaaat ccacttgcgc
     1621 cagagttctt tgccgggaaa gtcgaggaaa ctggtcggct catcgaggag gagatatttc
     1681 ggctgagtga cggagaccgc gcctaaaatg agccgctgct tttccccgcc ggaaaggtca
     1741 accggtggcc gcttgcgtaa ctccaccagg ccgtgagacg cgagtgtctc ttcgactttt
     1801 tcgcggatga cctgactcgg gaatcccctc tgttccaatc cgaaggcgat ttcccgctcg
     1861 acggtgggcg ccacgaactg gctgtccggg tcctggaaga cgatgcccat aagatcgcgc
     1921 agtttcgccc ggtcgctcct ggctttcgtg gacaagccgt cgatggtgac ttcccccgcc
     1981 gacgactcta acagaccggc gacggccaaa agcagaaccg tcttgcccga accgttggct
     2041 cccatcaccg ccactcgctc accgtctttg accgaaagac tgacgttgga cagggcgact
     2101 atcttttcgc ccttggccac cggcagggcg agagtgagat tttgaatttc aatcatgatt
     2161 cagacaaaaa acgccctgaa cgggcgatat gcaagcagat tttctgaaat acctcagcga
     2221 ggcgaaatgt tgattcccat ctctggagcg agagacgggg taactggcag gagtttatag
     2281 acctgcccct cgtcgggaat cggcaccaaa atcacgccaa aattgcccgt cgcttctctg
     2341 gcccacagtc gggctaatgg agtaagatcg atgacggctg tccggttacg tttgggcacg
     2401 atggttaccg tcgtcacgta aacatcattg aaat
//
LOCUS       DBNGZD010000036         2322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1672675, whole genome shotgun sequence.
ACCESSION   DBNGZD010000036 DBNGZD010000000
VERSION     DBNGZD010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1672675"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="AC16GM_01090"
     CDS             <1..622
                     /locus_tag="AC16GM_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR2922302.1"
                     /translation="EGAGALAKRVEKEVEQLGLAKTKFQVQIVQTQADDGVAVNGKTW
                     ALSETGIDQVEFLIAPNVGETLKPLAKIASGGELSRIMLALKSVASRADQVDTLVFDE
                     IDVGIGGEVAGAVGRKLKALAKTKQVLCITHLQQIAALADHHLLVSKEKEKNRMVTRI
                     KPLSPKERVPEIARMLSGEKPTALTLEHAEEMLKTAVRLAHRPEQS"
     gene            659..1141
                     /locus_tag="AC16GM_01095"
     CDS             659..1141
                     /locus_tag="AC16GM_01095"
                     /inference="COORDINATES: protein motif:HMM:NF018425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR2922303.1"
                     /translation="MRREFKPKFDRWLVALIFGPFLVIGIVLVIVSWAGTKSHPAQAG
                     EMLAPLFILAGVAIILISLMRSTRYILTASELVVKIGPIHFKFPYGTIQAVETGGAEK
                     LWSHGGALRMAFSNDNLVIKVAGRFIRSVVVSPEEKTEFLLLLKTYCPALAGESPARW
                     "
     gene            complement(1175..1627)
                     /locus_tag="AC16GM_01100"
     CDS             complement(1175..1627)
                     /locus_tag="AC16GM_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2922304.1"
                     /translation="MEAEVYPNDRVAQLISANFLPVKIHVKEQPQTFGRFNAKWTPTL
                     IVLDSDGTERHRIEGFLPLDDFLAQLELGLAKLSFEHLQYGEAEKRFRSIQQKYAASG
                     AAPEAAYWAGVSAYKATNKPEPLGDTAKLLKVKYPDSEWTRKASVWIH"
     gene            complement(1906..>2322)
                     /gene="mscL"
                     /locus_tag="AC16GM_01105"
     CDS             complement(1906..>2322)
                     /gene="mscL"
                     /locus_tag="AC16GM_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012685240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR2922305.1"
                     /translation="EFAMRGNVLDMAIGIIIGAAFGKIVTSLVNDVIMPPLGLVIGKV
                     DFSNLFVNLSGQHYATLAEAKAAGASTIGYGVFLNTVIDFLIVAFVIFLLIKQVNRMK
                     RQPAAVPTTKECPHCLSAIALKATRCAYCTADLKVA"
BASE COUNT          492 a          674 c          671 g          485 t
ORIGIN      
        1 cgaaggcgcc ggcgctctgg ccaaacgcgt ggagaaagag gtagagcagt tgggactggc
       61 gaagacgaag tttcaggttc agatcgtgca gacccaagcg gacgatgggg tagcggtcaa
      121 tggcaaaact tgggctttat cggaaaccgg catcgatcaa gtagaattct tgattgctcc
      181 caatgtcgga gaaacactca agcccctggc aaagattgcc tccggcggcg aactctcccg
      241 cattatgctg gctttgaagt cggttgcctc ccgggctgat caggtcgata cgctggtgtt
      301 cgatgaaatt gacgtcggca tcggcgggga agtggccggc gccgtggggc gcaagctgaa
      361 agcgttggcg aaaaccaagc aagtgctctg catcacccat ctccagcaaa ttgcagcctt
      421 ggcggatcac catttgctcg tttccaaaga aaaggagaaa aaccggatgg tgacgcggat
      481 caagccgctg agcccgaaag aacgggtgcc ggaaatcgcc cggatgctct ccggcgaaaa
      541 gccgactgcc ctgaccctcg agcacgccga agaaatgctc aaaacagcgg ttcgactggc
      601 tcaccgcccc gaacaaagct gaggggcggc gcgtccttaa gcaaaccgga actcagatat
      661 gcgccgtgaa ttcaaaccga aattcgaccg ctggctcgtg gccttgatct tcggcccgtt
      721 tctggtgatt ggaattgttc tggtgattgt ttcctgggcc ggcaccaaaa gccaccccgc
      781 ccaagcgggg gagatgctcg cgccgctttt catcctggcc ggagtcgcca tcatcctcat
      841 ttccctcatg cgctctaccc gttacattct gaccgcgagc gaactggtcg tgaagatcgg
      901 gccgatccac ttcaaattcc cgtacggcac cattcaggcc gtcgaaaccg gcggcgcgga
      961 aaaactctgg agccacggcg gagcgctgcg gatggccttt tcgaatgaca acctcgtgat
     1021 taaagtggcg ggccggttca tccgctcggt ggtggtgagc ccggaggaaa aaactgaatt
     1081 tcttttgctc ctgaaaacgt actgccccgc ccttgccgga gagtctcccg cccgttggta
     1141 gagaggctct ccggaaatta gcgctccgct tcgttcaatg aatccagacc gacgctttgc
     1201 gcgtccattc gctgtcggga tacttcactt tgaggagctt ggcggtatcg cctaacggct
     1261 ccggtttatt cgtcgctttg taggccgaca cgcccgccca gtaggccgct tccggcgccg
     1321 caccgcttgc ggcatacttt tgctgaatcg agcggaagcg cttctccgcc tcgccgtatt
     1381 gaaggtgctc gaacgacagt tttgccagcc ctaattccaa ctgcgccaaa aaatcgtcca
     1441 gcggcagaaa tccttcgatc cggtgccttt cggtgccgtc ggagtcgagc acaattagag
     1501 tcggcgtcca cttggcgttg aaccgtccga aggtctgggg ttgctccttg acgtggattt
     1561 tgaccggcag gaaattcgct gaaatcaact gcgccacccg gtcgttgggg tagacctcgg
     1621 cttccagccg agcgcacccg cttcacatcg gcgcggcgct gaaatccaga agcaccggct
     1681 tctgcgcttt tttcgcttcg gtgagcgttg aatcgacgtc tttggaccag ttaatcgcgg
     1741 ccatcacaaa cctcctctgt tcgcagtata tacgctaccg aataggtttt gtttatacag
     1801 gcaggttttg tcggggctct catttccctc cccttgaggg tcgaagactc gtaccgagga
     1861 gggcaggcca tacggcccgt atggggaggg gtcagcgtaa atgcattacg ccaccttcaa
     1921 gtccgccgtg cagtacgcac agcgcgtcgc cttgagcgca atggcggaca ggcagtgcgg
     1981 acactccttg gtcgtcggca ccgccgccgg ctgccttttc atccggttga cctgtttgat
     2041 caaaaggaaa atgacgaaag ccacgatcag gaagtcgatc accgtgttaa ggaatacgcc
     2101 gtagccgatg gtcgaggcgc cggcggcctt ggcctccgcc agcgtggcgt agtgctgccc
     2161 ggagagattg acgaacaggt tggaaaaatc aaccttgccg atgacgaggc ccaacggcgg
     2221 catgatgacg tcattgacaa gagacgtaac gattttcccg aacgccgccc cgatgatgat
     2281 cccgattgcc atatcaagca cgttcccgcg catggcaaac tc
//
LOCUS       DBNGZD010000037         3871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_144621, whole genome shotgun sequence.
ACCESSION   DBNGZD010000037 DBNGZD010000000
VERSION     DBNGZD010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_144621"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="AC16GM_01110"
     CDS             <1..1442
                     /locus_tag="AC16GM_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922306.1"
                     /translation="QTFLDQINKFVDLWNSLEIRVFAAKIGKDWLNLGSVFFLNWKRQ
                     DEISFHPPLQREDELKIIHKVDAPGNLLQYLEGLGIGKLRINDIEIIYGRVRNAVIVP
                     YSFYLRPPKRNRDEVFPEERDFKVLTLEGYNDSLMDVLGDKYGRLNNSLKTNTPHFSN
                     FRGVANDFLKSHYPLDDLYAHTFFEVLAPVPIRLGDQCRAEKNTLLVHSEAQIPVNGS
                     DLRLNLTKRSAYEPRTEKFEISGKKANRMGDKDLIWEIDLQRGSSYEVDLIFRDDQID
                     SLTIKALSTSPRVVLHEIWDENYEIIDKLLSEKTKKTSKGKKPIRNEEFEIAVSLLLY
                     LCGFSSGHYGTIAEYGEGVDILAFSSNDKFLLAGECTVEFPDKKGKLQNFYKRVQELK
                     DHTDLEVVPVLFTRLPLNKIPLDEKNRAGQNKISILSNERIQRLLSMARKGSSVKEYH
                     EFFKNPEPLDLEYISAKGVQLSFRHSRGF"
     gene            1489..3456
                     /locus_tag="AC16GM_01115"
     CDS             1489..3456
                     /locus_tag="AC16GM_01115"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR2922307.1"
                     /translation="MPMNLTQKLISSHLVNGGADPSTLAQGGGSIPSTTKPGEEIAIK
                     IDQTLLQDATGTMAMLEFEALGVPRIKVKRALVYVDHNMLQTGFENPDDHLFLQTMAA
                     KYGAIFSRPGNGISHQAGLERFDAPGETLLGADSHTPQAGALAMLAIGAGGFEVAAAM
                     AGEPYRFRMPEIVLVRLSGALKPWCSAKDVILELLRRFTVKGGVGKIFEYGGPGLAAL
                     TVPARSTIANMGAELGLTSSVFPSDEQAQRFLKAQKREKDFKPLVADDGAKYDDVVEL
                     DLSKLEPLIAQPKSPDKVVPVREIEGTEVKQVAVGSSVNSSFVDLMTAAKIMEGKKVH
                     PGLHMTISPGSRQVLIMATEAGGMAALFRAGARGLEVACGPCIGMGMAPPSKGKSVRT
                     FNRNFPGRSGTADDEVYLTSPEVAAATALNGVITDPRKLGTPPKIEMPEEFVIDDSGF
                     LPPAPDPGKVEIVRGPNIQRLPIPPQLDDAIKGVVLIKLKDNISTDEILPAGGKILPL
                     RSNIPAIAEYVFHYVDPDFPARAKAKGPGIIVAGDNYGQGSSREHAALAPMFLGVRAV
                     VAKGFARIHQDNLVNFGLIPLRFTKPEDYDALAQDDEVTLLNIRDCLKDKRPILLRNV
                     TRKREMLLEYKLTPRQIEIILAGGLVHYLKR"
BASE COUNT          978 a          921 c         1088 g          884 t
ORIGIN      
        1 tccaaacatt tctagatcaa attaataagt tcgtagacct ctggaatagt cttgaaatcc
       61 gtgtgtttgc tgctaagata ggtaaggatt ggttaaatct tggttctgtt tttttcctga
      121 actggaaaag acaagatgaa atttcttttc atcctccact acaaagggaa gatgagctaa
      181 agattattca caaggttgat gctcctggta accttttaca atatcttgaa ggattgggaa
      241 tcggaaagtt acggataaat gacatcgaaa taatctacgg cagagtcaga aacgcggtaa
      301 tagtgccgta tagtttctac ctccgacctc caaagagaaa tcgcgatgaa gtattccctg
      361 aggaacgaga tttcaaggtg ctaacattgg aaggatataa tgactcactt atggatgtgt
      421 taggtgataa atacggtaga ttaaataact ctcttaaaac aaatactcct catttttcaa
      481 attttcgcgg ggttgctaat gattttttga aaagtcatta ccctcttgac gacctctacg
      541 ctcacacatt ctttgaagta ctagcacctg tgcctatccg cttaggcgac caatgcagag
      601 ctgaaaaaaa tacattactt gttcacagcg aagctcaaat ccccgtaaat ggctctgatc
      661 ttcgactaaa tcttacaaag cgtagtgctt acgagcctcg gaccgagaag tttgagatct
      721 ctggaaagaa agccaataga atgggagaca aagaccttat ttgggaaata gatttgcaaa
      781 gaggatcgtc ctacgaagtc gatcttattt ttagagatga ccaaatcgat tccctaacaa
      841 taaaggcact gtcaacaagc cctagagtag tattacatga aatatgggac gagaattatg
      901 aaatcattga caaactctta tctgaaaaaa ccaagaaaac atccaaaggt aaaaagccta
      961 taagaaacga agaatttgaa atagcggtaa gtttgttgct ctacttatgc ggattttcca
     1021 gtggtcacta cggtacgata gcagaatatg gtgaaggagt cgatatactc gcattttcta
     1081 gtaatgataa atttttattg gccggtgaat gcaccgttga atttcctgat aaaaagggga
     1141 aactgcaaaa tttttacaag cgggtccaag aacttaaaga ccatactgac ttggaagtag
     1201 tcccggtact ttttactcga ttacctctta ataagatacc tttggacgaa aagaaccgtg
     1261 ctgggcaaaa caagatttct atcttatcca atgagagaat tcaaaggtta ctttccatgg
     1321 cacgaaaagg atcttctgtt aaagaatacc acgaattttt taagaacccc gagccactcg
     1381 acttagagta tataagtgca aaaggcgttc agttgagttt tcgtcatagt cgaggtttct
     1441 aaaagacttt tctgttaaag gttatagaaa acgtgaggga aaactgacat gcccatgaac
     1501 ctgacgcaga agttgatctc ctcccattta gttaatggcg gggccgaccc ttcgacttta
     1561 gctcagggtg gtggctcgat tccgagcacc accaagcccg gcgaggagat tgccatcaaa
     1621 atcgaccaga cgctgttgca ggatgccacc ggcaccatgg cgatgctgga gttcgaggcc
     1681 ttgggcgtgc cgcggattaa ggtgaagcgg gctttagtct acgtggacca caacatgctc
     1741 cagaccggct ttgagaaccc ggacgaccac ctctttttgc agacgatggc ggctaagtac
     1801 ggggcgattt tctcccggcc ggggaacggc atctcgcacc aggcgggctt ggagcggttc
     1861 gacgcgccgg gcgaaacgct tttgggggcc gacagccaca cgccgcaggc cggggctttg
     1921 gcgatgcttg ccatcggcgc gggcgggttc gaggtggcgg cggcgatggc cggggagccg
     1981 taccggttca gaatgccgga aatcgttttg gtgcgcctct ccggcgcgct caagccctgg
     2041 tgctcggcca aggatgttat tttggaactt ttgcggcggt ttaccgtcaa aggcggcgtg
     2101 gggaagattt ttgaatacgg cggcccggga ctggcggcgc tcaccgtgcc ggcgcggtca
     2161 accatcgcca atatgggtgc ggaattgggg ctgacgagtt cggttttccc gtcggatgag
     2221 caggcgcagc ggttcctcaa agcgcagaag cgggagaaag atttcaagcc gctcgtcgcc
     2281 gacgatgggg ccaaatacga cgacgtggtg gagttggatc tctcgaaact tgagccattg
     2341 atcgcccagc cgaaaagtcc ggacaaggtc gtgccagtgc gggagattga aggcacggag
     2401 gtcaagcagg tggcggtggg gagttcggtc aattcgtcct tcgtcgattt gatgacggcg
     2461 gcaaaaatca tggaggggaa gaaagtccac cctggcctgc acatgacgat ttcgcccggc
     2521 tcgcgtcagg ttttgattat ggcgactgaa gcgggcggga tggcggcgct ctttcgggcc
     2581 ggcgcgcgcg ggctggaggt cgcctgcggg ccctgcatcg ggatggggat ggctccgcct
     2641 tccaagggca agtcggtgcg gactttcaac cgcaacttcc ccggccgaag cggaacggcg
     2701 gatgacgaag tctatttgac cagcccggaa gtggcggcgg cgacggcttt gaacggcgtt
     2761 atcaccgatc cccggaaact cggcacgccg cccaaaatcg aaatgccgga ggaatttgtg
     2821 atcgacgact ccggtttcct cccccctgcg cccgatccgg gcaaagtcga aatcgtccgc
     2881 gggccgaaca tccagcgttt gcccatcccg ccgcagttgg acgacgcaat caaaggcgtc
     2941 gtgctcataa aactcaaaga caacatctcc accgacgaga ttctcccggc gggcgggaag
     3001 attctcccgc tgcgttcgaa catcccggcc attgccgagt atgttttcca ttacgtcgat
     3061 cccgatttcc cggcgcgggc gaaagcgaaa ggcccgggga ttattgtggc gggcgacaac
     3121 tacggccagg gctcgagccg ggagcacgcg gccctggcgc cgatgttctt gggtgtgcgg
     3181 gcggtggtgg ccaaggggtt tgcccgcata catcaggaca atttggtgaa cttcgggctg
     3241 atcccgctcc gcttcaccaa gccggaagac tacgacgcgt tggctcagga tgatgaggtc
     3301 accctgctca acatccgtga ctgcctgaag gacaaacgcc ccattctcct gcgaaatgtg
     3361 accaggaagc gggagatgct tctggaatac aaactgacgc cgaggcagat tgaaatcatt
     3421 ttagcgggcg ggctggtgca ttatttgaaa aggtgaagcg gagtgctgac ccctccctcg
     3481 ccctcccctc aaggggaggg aaattgcaag ggaaaggtgt tgtcgtgtag gggcgaatct
     3541 ttacctgtcc tgagcgaagt cgaaggcgat tcgtccggag ggtgtgaacg gttaactgat
     3601 ttagaacctg ccgtaaggcc ggttctcaga aactcaaaag aacatacctc aggcgggtgt
     3661 ccctgaaggt tgcccccaca tgcttgaagc gatgagagta gaatttcgac cccttccttt
     3721 ttccctcccc tcggagggga gggtggggtg gggtcaggcg catagggttg cgggcgaaca
     3781 cgggggttcg cccctacaaa ctctcgcggg ttcttaagaa aggaggatca agaaatgctg
     3841 aaagagttca aggagtttgc catgcgcggg a
//
LOCUS       DBNGZD010000038        12174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1325564, whole genome shotgun sequence.
ACCESSION   DBNGZD010000038 DBNGZD010000000
VERSION     DBNGZD010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1325564"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..211)
                     /locus_tag="AC16GM_01120"
     tRNA            complement(122..211)
                     /locus_tag="AC16GM_01120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(174..176),aa:Ser,seq:gct)
     gene            complement(248..535)
                     /locus_tag="AC16GM_01125"
     CDS             complement(248..535)
                     /locus_tag="AC16GM_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2922308.1"
                     /translation="MYYVYILRFVGTGRFYIGQTQNLERRLLKHQQGKTKSVKNRGQF
                     EVIFVDRFATRAQAMRREKQIKSYKGGQAFEKLLQARTSLAKLVEVPPLAG"
     gene            complement(817..3441)
                     /locus_tag="AC16GM_01130"
     CDS             complement(817..3441)
                     /locus_tag="AC16GM_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2922309.1"
                     /translation="MRPFSTPRWRIFSHLVLPGGLFWLVGLFPGTTARAATTVATLAN
                     GMQVVMKENHVNPVIGLTVYVKAGSKYEGETTNGFTHLLEHLLFDGSRKYSREEINEG
                     MKAHGGYINAFTRKELTGFLTLVPGEEFDFALDLLDEMLFHSIFPDEELAKERKVVIE
                     EIRKDEDNPDYEVERFFDSQVYAGTPYARPVLGYPRYIEQVAREEIISYWQRRYVPGN
                     MVAVVVGDFEPEEMLKQLQSVFGSEPAQPVSPETAVRWLLPKEVRVLRKQLDVQTTYL
                     NFAFAAPHFSDPDYIPFSFLTEILNSGEASPLNQALTSGSDPLALTCTAALETQKEFS
                     ALTLSVTTDRAEKADSIIGAVKQYFSLPGGFSFTADQIQATLVTLETGEEFLLEKPHY
                     YGLMRAPFYVTTGYAFLESYWDRMAKVTPRDLQRVAQKYFNPPVFTTTVVTPIQKAPK
                     GTSEQSSTSLQRKLLPNGLEVIIKSNPDSKVFAFTLLGKYRSAQERDDQAGMTDFIHR
                     MLARGTQSRSKEAIASVLRAMGAELTTNDNPAIPYDDIYTTRAYSFIKLQTVDRFADS
                     ALALVADLVLHPLFAAGEIAKTKREVFDLLRREEQSPTQAARNLFYRTIFAGGPYAKT
                     VMGDSATVAGFTPDEIAAYHRTYFSGGNLILAVATGLPATSVLEKIKTFFASLPTLSR
                     ELPQVIRPEAPAELHEVEKPLKKEQISICVGTLLPGANDPDIPALQVMNAILSARLGL
                     ELREKQGLAYSVGSSVTFDRNFGWLMIQLGTGKGNFALAQKGISGEIEKLQNGGVGDD
                     ELQKARKSLWGNYLLANLSRLNQTYWMAVNQFIGRGFDYDQKFVARLEAVTTKDVQRA
                     AQQYLNLKHAVLTTAGMR"
     gene            3687..4721
                     /locus_tag="AC16GM_01135"
     CDS             3687..4721
                     /locus_tag="AC16GM_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR2922310.1"
                     /translation="MAKSDSVTDDLFLSCGNAKISGSVTGDLMGFGGSLVFEGPVKGS
                     VMAAGRSVELSGPITGSVRVAGQNLVVNTHVVRNLMAAGQTIHLGSETKIAGDAHLAG
                     EEILIDGQVLGNLYASGARVVILGHIGKNVSLKAEKITLMPTAVIEGNLKYRSRKEAK
                     VEKGALVKGEIKWLPLKRKAPKPFLTAGKVFGKLFFYVAMLIVGSLIIALFRKQTYAV
                     KELALTSFLKSFGIGLLGLVAALVGGLLCLVTLIGAPLALITFTLTFLTVYTTKIFIS
                     IAVGDWVLKLFQKTGSPSPYWAFVVGLFLVTILVSLPYVGWLFYFVVLFTGFGAILLS
                     LKPLIPQKMG"
     gene            complement(4791..7580)
                     /locus_tag="AC16GM_01140"
     CDS             complement(4791..7580)
                     /locus_tag="AC16GM_01140"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2922311.1"
                     /translation="MKRSMVGWFILWVLLFTAAQAQPNFGVTKQVLDNGLVVLVKEKH
                     DIPVVSCMVWYKVGGRNEEIGKTGVSHFLEHMLFKGTKKLKKGDIDLITLKNGGSNNA
                     FTSNDYTAYYFNFASDRWEEALEIEADRMVNNQFLPEEFNPEKQVVIEELKRGNDGPW
                     DQLFEDFQAAAFKAHPYQYPVIGWITDLDKLSIADMEAYYRTFYQPANAILVIVGDVN
                     TEKTLEKVRQLFGTLERRGAIPDMRAVEPEQLGERRIIVHKESNLPRLLVGWHTPKVG
                     EKDSYPLTVLATILSGGKSSRLYQELVEKEKAVTFVNAFYDDARDPSLFAVQAELQPG
                     HELADVEKSIYTQIDRLAAGGISDSELVKAKNQTEAGFVFDQETASGQANALGRAAAI
                     FNYAYAFSYVDSIRKVTREEVVRVARKYLVPDNRTVGWLVPKGGSPESTEQGAKGSTD
                     QFFGAVERDAAAVPAGQVLLQRRKAYKSSDRAYPLQTPPAQTTPPAHSIVAPAKLNVS
                     QKTLGNGLTVLLLERRGNPTVAINAFVKAGSRFDPEEKAGLANLTARMLDEGTTARTG
                     LEIAQAIDFVGGSLSTSGGYAGSTVSAQVLSKDLDLGLNLVADVVRRPVFDSAKVEDE
                     KQKIAAEIKSSLENPRTVGSLQLNELVYGKHPLHRPSVGYEETVAAITRQDVVNFHKF
                     FYLPNDALLAVVGDFKAADVIKKIEKYFGTWEKRSLALPVVPEIARQSDERHRYRYMD
                     KEQINIYLGHLGIKRDNPDYYALEVFDTMLGGGPGFTSRIPKNLRDNLGLAYTTFSNI
                     TGTAGIDPGRFVAYIGTSIPNKDKALEGLKNEIKQFLGGPLGDDELEFAKAYLTGSFV
                     FDFETNSQIAGFLINAVLYNLGFDYLAKYPKLIEAVTKADVARVAKKYIDLDHMSLVI
                     VGKVNERGEVVETKP"
     gene            complement(7809..9008)
                     /locus_tag="AC16GM_01145"
     CDS             complement(7809..9008)
                     /locus_tag="AC16GM_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR2922312.1"
                     /translation="MPVFEYKGKTAAGVAVSGELKAKSRAELERILRTNRILVSSLSR
                     KPSQIKLSFGTGIKKIHISRFTRQFATMISAGLPMVQCLEILAAQSESKELRQVIGEI
                     KDSVSSGSTLCEAMGKHKKVYDDLYVNMVEAGEVGGALDSILNRLAAYREKADALTRK
                     VKGAMVYPAVIVVVAILVTTLMLVKIVPIFAKMFQGLGSELPGPTQFVLKISSILRGN
                     IFFIILGAGALVAFIKLWGRTDGGRLAIDKFKLRLPLLGTLIRKTAIARFTRTLGTLI
                     SSGVSILDALETTAKTAGNRVIHDAIKKSVVAIAQGDTITGPLRESGVFPPMVTQMIS
                     VGEKTGGLDEMLGKIADFYDEEVDAAVAALTSIIEPSIIVVMGTVIGGILIAMYLPMF
                     DIIGQIK"
     gene            complement(9024..10100)
                     /locus_tag="AC16GM_01150"
     CDS             complement(9024..10100)
                     /locus_tag="AC16GM_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR2922313.1"
                     /translation="MTSLRDLLELMVKRNASDLHLTVGAPPMLRIDGKLARTDLEMLT
                     PEMTKKLAYSIMNEKQRQKFEEKSELDLSFGIEQLSRFRCNIFMQRGNVAAAIRQIPF
                     RIQSFEELGIPKVVSEFANLPKGLVLVCGPTGSGKSTTLAALIDKINRERHDHIITVE
                     DPIEYLHRHNLCLINQREVHADTQSFASALKYALREDPDVVLIGEMRDLETVEAALNI
                     SETGHLAFATLHTNSAMEATTRIIDVFPNNQQPQVRVTLSFVLQAVLCQQLIPKIGGG
                     RVLALEIMVCTPAIRALIRDDKIHQIYSLIQAGQKYGMKTMNQSLSELVQTRKITQGE
                     AFGRSTNVQELSEMLARRQELAYA"
     gene            10545..11726
                     /locus_tag="AC16GM_01155"
     CDS             10545..11726
                     /locus_tag="AC16GM_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2922314.1"
                     /translation="MVKEQADLSSLRIERSSDYRPPRKWWRWVLGLSIVPLLVIGYLF
                     VMHQVAPGLAVQVGTATLITGSQAQALLTASGYVVAQRKAAVASKATGRLEYLGVVEG
                     DRIKAGMVIARLEKADITAELDRAKANLAHAVAESTEATLFYERQKKMVADSLISNLE
                     FEGAEARYQRALAGVASGRAAVKAAEVALENTVIRAPFDGTVLTKSAEVGEMVAPFAS
                     TASSRGAVVTLADMNSLEVEADVAEANIERVQVGLPCEITLDAYPDTRYPGKVSKIVP
                     TADRTRATVLVKVAFLSRDRRVLPEMSAKVNFLPENTQLSAQNTEPVVAVPNSAVTMR
                     NGVKMVFLVEGNLVKSVPVTTGRVLGDMTEIKAGLQAADRVVISPPGSLATGMKVKTS
                     E"
     gene            11746..>12174
                     /locus_tag="AC16GM_01160"
     CDS             11746..>12174
                     /locus_tag="AC16GM_01160"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2922315.1"
                     /translation="MAESIVEIEALSKSYYRDSLEIPVLHNLNFSIPEGEFVALMGPS
                     GSGKTTLLNMIAGIDRPTKGNVKVAGTDLGRLGEGELARWRARHIGFVFQFYNLMPVL
                     TAYENVELPLLLTNLSKKERRKHVETTLGIVGLADRMKHYP"
BASE COUNT         2429 a         3503 c         3528 g         2714 t
ORIGIN      
        1 aaccatctgt gcagcgtaag atgtacacag accaggatga atcttgctcc aacacggttt
       61 tacccttcga cttcgctccc atcgggtctg agaccctcag ggtcgatgac agggttttca
      121 acggagaggc tgggattcga acccagggtc ccgatttctc ggaacaactg cttagcaggc
      181 agctgccttc agccagctcg gccacctctc ctaaaatcat ccgccatttc gctccaccac
      241 catctgctta tcccgccaga ggcgggactt caaccagctt ggccaatgat gttcgagctt
      301 gtaacaactt ctcaaatgct tgacctcctt tgtagctctt gatctgcttt tctcttctca
      361 tggcctgagc ccgggttgca aatctgtcca caaatattac ctcaaactgc cctcgattct
      421 taaccgactt tgtctttccc tgttggtgct taagtaaacg cctctctaag ttttgtgtct
      481 gcccgatgta gaatcgtccc gtccctacga agcgcaggat gtatacgtag tacatgtttt
      541 cagataacca gagaggttgg gattcgaacc cagggtcccg atttctcgga acaactgctt
      601 atcccgccgc aggcgggact taagccaact cggccacctc tgcttagttc aaggttttct
      661 caaccgacac caactccaac tgcttatctc ccgcttttgg cgggacggga cttcagtccc
      721 gcaaaatgcg ggaccacctc tccagattcg gcggacaaaa aagcttcgcc cgatccaaaa
      781 gtacgttgtc cggctcactc ctccaaataa ccctcctcac cgcatccccg ctgttgtcaa
      841 aacggcgtgt ttcaagttga gatactgttg cgctgcccgc tggacgtctt tcgtagtcac
      901 ggcctccagg cgcgcgacga acttctgatc gtagtcaaat ccccgaccga tgaattgatt
      961 caccgccatc cagtaggttt gattcagacg cgacagattg gccaataggt agtttcccca
     1021 gagtgatttc ctggctttct gcaattcgtc atcgccgacg cccccgtttt gcaacttttc
     1081 gatttcgccg ctaattcctt tctgcgccag agcgaagttc cccttgcccg ttcccaactg
     1141 gatcataagc cagccgaagt tgcggtcgaa agtgaccgac gaccccaccg aataggccag
     1201 cccctgcttt tcccgcagtt ccagccccag ccgggccgag aggatggcat tcatcacctg
     1261 gagtgccggg atgtcgggat catttgcgcc cggcaaaagc gtcccgacgc agatggagat
     1321 ttgttctttc ttcagcggct tttccacctc gtgcaattct gccggcgcct ccggccggat
     1381 aacctgcggc agttcacgcg acagggttgg gagtgaggca aagaaggttt taatcttctc
     1441 cagtaccgac gtggctggca gcccagtcgc taccgccagg atcagatttc cgccggaaaa
     1501 gtaggtgcgg tggtaagcag cgatctcgtc cggtgtgaac ccggccaccg tggcggaatc
     1561 ccccatcact gtcttggcat aggggccgcc ggcaaaaatc gttcggtaga agagattgcg
     1621 ggcggcctgg gtcggcgact gctcctctcg gcgcaggagg tcaaagacct cgcgtttggt
     1681 tttagcgatt tcgccggcgg cgaaaagcgg gtgcaagacg agatcggcca ccagtgccaa
     1741 cgccgaatcg gcgaaccgat cgaccgtttg gagcttgatg aaggaatagg cgcgggtggt
     1801 gtaaatgtcg tcgtagggaa tggcgggatt gtcgttcgtc gtcaactccg ctcccatcgc
     1861 ccgcaggacg gaagcgattg cttctttgga acgtgattgc gtcccgcgcg ccagcatccg
     1921 gtgaatgaaa tcggtcatcc cggcctgatc atctcgttcc tgggccgacc ggtacttccc
     1981 cagaagagtg aaggcaaaga ccttggaatc ggggttgctt ttgataatga cttccagacc
     2041 gttcgggagc agtttgcgtt gcaaggaggt gctggattgt tccgacgtcc cctttggagc
     2101 tttctggatt ggtgtcacca ccgtcgtagt gaaaaccggc gggttgaaat acttctgcgc
     2161 cacgcgctgg agatcccgcg gtgtcacctt tgccatcctg tcccaatacg actccaggaa
     2221 tgcgtagccg gtggtcacgt agaacggcgc ccgcatcagg ccgtagtagt gcggtttttc
     2281 caggaggaac tcttccccgg tttccaaagt cacgagcgtc gcttggattt ggtccgcggt
     2341 gaaggagaaa ccccccggaa gagaaaagta ctgcttcacc gcaccgatta tggaatcggc
     2401 tttttccgcc cggtcggtgg tgaccgaaag ggtcagtgcc gaaaattctt tttgtgtttc
     2461 cagtgccgct gtacaggtga gcgcgagcgg atcggaaccc gaggtcagtg cttggttcaa
     2521 cggcgaggcc tctcccgagt tcaagatttc ggttaaaaaa ctaaagggaa tgtaatcggg
     2581 gtctgaaaaa tgaggcgccg caaaagcaaa attgaggtaa gtcgtctgca cgtcgagttg
     2641 cttccggagg acccgaactt ccttcggtaa caaccagcgt accgcagtct cgggcgacac
     2701 cggttgcgcc ggctccgagc caaaaactga ttgaagttgt ttcagcattt cctcaggctc
     2761 aaaatccccc accaccaccg ccaccatatt gccgggaacg tagcggcgtt gccagtagct
     2821 gataatttct tcccgggcaa cctgttcgat gtagcgcggg taccccagaa ccggccgggc
     2881 gtagggcgtc ccggcataga cctgggagtc gaagaaccgc tccacctcgt agtcgggatt
     2941 atcctcatcc ttgcggattt cctcgatgac caccttccgc tctttggcca gttcctcatc
     3001 ggggaagatt gagtggaaga gcatctcgtc gagcagatcc agtgcaaaat cgaattcttc
     3061 tccggggacg agcgtcagga atccggtcag ttccttgcgg gtaaaggcat tgatgtaacc
     3121 gccgtgtgct ttcatcccct cgttaatctc ttcccggctg tatttgcggc tgccgtcgaa
     3181 gaggaggtgc tccagaagat gggtgaagcc gttggtcgtt tccccctcat acttcgaacc
     3241 ggccttgacg tacacggtta ggccgatgac cggattgaca tgattctctt tcatcactac
     3301 ctgcatgccg ttggccagag tcgcgaccgt cgtcgcggcc cgggcagttg tgcccgggaa
     3361 gagcccgacc agccagaaca gccctcccgg taggacgaga tgggagaaaa tacgccaccg
     3421 cggagtagaa aaaggtcgca tgcccgcaaa aatatgcccg ccgcgaagaa aaacaaaact
     3481 aaagcagctg gaaaccgtaa gagaacttgc cagcttgcgc accctcgtgg tatatatgac
     3541 ctgcgactga ggacgaatta tctccggagc gttggaggag gatgactatg agcaagaccc
     3601 atatgacgtt cgcgacggtt tgcgcggcca tcttttttgc ttctcccctt ttcgccacgc
     3661 tgtttcgcgg cgggtaaagc gtggtcgtgg ccaaaagcga ctcggtcacc gatgaccttt
     3721 tcttaagctg cggcaacgcg aaaatttcgg gcagcgtgac aggcgacctt atggggtttg
     3781 gcggctcgct cgtcttcgaa ggccccgtaa aagggagcgt gatggccgcc ggccgctcgg
     3841 tggaactctc cggcccgatt accggttctg tccgcgtggc cggtcagaac ctggtggtca
     3901 acacccacgt cgttcgcaac ctcatggccg cgggccagac catccatctg ggttcggaga
     3961 ccaaaatcgc gggcgacgcc catctggccg gggaagagat tttgattgac ggtcaggtgc
     4021 tgggaaacct gtacgcctcc ggcgcccggg tggtcattct cggccacatt gggaaaaacg
     4081 tctcgctgaa agctgaaaaa attacactga tgccgaccgc cgtcatcgaa ggcaacctga
     4141 agtaccggag tagaaaagaa gccaaagttg agaaaggcgc actggtcaaa ggggaaataa
     4201 agtggctgcc gctgaagagg aaggcgccca aaccctttct caccgccggg aaagtcttcg
     4261 ggaaattatt cttctacgtc gccatgctga tcgtcggaag cttgatcatt gcgcttttcc
     4321 gcaagcagac ctatgccgta aaagaactgg cgttgacctc gttcctgaaa agttttggca
     4381 tcggtctact cgggctggtt gccgcgctgg tgggcggtct cctctgtctg gtgaccctca
     4441 tcggcgcgcc tctggcgctt atcaccttca cgctgacgtt cttgaccgtc tacaccacga
     4501 aaatttttat cagcattgcc gtcggtgact gggtcctgaa actcttccag aagaccggtt
     4561 ccccttcccc ctattgggct tttgtcgtgg gcttgttcct ggtgacgatc ctggtgagtc
     4621 ttccctacgt gggctggctc ttttactttg tcgtcctctt caccggtttc ggggcgatcc
     4681 tcctgagtct taagcccttg atcccgcaga aaatggggta accccgcaag ttcgggacag
     4741 agaaaagcgc cggcgggcgc ttttcttttt tgcgctgctt gatggtaaaa ctacggcttc
     4801 gtttcgacca cctctccccg ctcgttcact ttgcccacga tgaccagaga catgtggtct
     4861 aaatcgatgt actttttggc cacgcgcgcg acatctgcct tggtcaccgc ttcgatcagt
     4921 ttcggatatt tcgccagata gtcgaaaccg aggttgtaga gcaccgcgtt gatcaaaaaa
     4981 ccggctattt gggaattggt ttcgaaatcg aacacgaaac tgccggtgag gtaggccttg
     5041 gcaaactcta attcatcgtc gcccagaggc ccgcccagaa attgcttgat ctcgtttttc
     5101 agcccttcga gggctttgtc cttgttcgga atcgacgtgc cgatgtaggc cacaaagcgt
     5161 cccgggtcga tgccggcggt accggtgatg ttggagaagg tggtgtaggc caaccccaaa
     5221 ttgtcgcgca ggtttttggg gatgcgcgag gtgaaccccg gcccgccccc cagcatcgtg
     5281 tcgaacactt ccagagcgta gtagtccggg ttgtcgcgct ttatgcccag gtgtcccagg
     5341 tagatgttga tttgctcttt gtccatgtag cggtaccggt gccgctcgtc gctctgccgg
     5401 gcaatctccg gcaccaccgg cagggccagc gaccgcttct cccacgtacc gaagtatttc
     5461 tctattttct ttatgacgtc agcggctttg aaatcgccga cgacggccaa aagtgcgtca
     5521 ttcggcaggt agaagaattt atggaaattc accacgtctt gccgcgtgat ggcggccacc
     5581 gtctcttcgt accccaccga cggccggtgg agggggtgct tgccgtaaac gagttcgttc
     5641 agttgcagtg atccgaccgt gcgcggattc tccagagaac ttttgatctc cgcagcaatc
     5701 ttctgtttct catcctcgac tttggccgaa tcgaacaccg ggcgtcggac gacgtcggcc
     5761 actaagttca agcccagatc gaggtctttg ctcaacacct gggccgagac ggtggaaccg
     5821 gcgtagcccc cggaggtgga aagcgacccg cctacgaagt caatcgcctg agcgatctcg
     5881 agaccggtgc gggcggtggt gccttcgtct aacatccgcg ccgtcaggtt ggctaaacct
     5941 gctttttcct cggggtcaaa gcgtgacccg gctttgacga aggcgttgat cgccacggtc
     6001 ggattgcccc ggcgctccag gagcaaaacg gtcaggccgt tgcccaaggt cttctggctg
     6061 acgtttagtt tcgccggagc aactatggag tgagccggag gtgtggtttg ggctggcgga
     6121 gtttgaaggg ggtaagcgcg atccgaggac ttataggcct tgcgccgttg caacaaaact
     6181 tgacccgctg gaaccgccgc ggcatcccgc tccactgctc cgaaaaattg atcagttgat
     6241 cccttggccc cttgctccgt tgactctgga gaaccacctt tgggcacgag ccaccctacc
     6301 gtccggttgt ccggaaccag atatttccgg gcgacgcgaa cgacttcttc tcgggtgact
     6361 ttgcggatgc tgtcgacgta gctgaaggcg taggcgtaat tgaagatcgc cgcggcccgg
     6421 cccagggcgt tcgcttggcc ggaggcggtt tcctggtcga agacaaagcc cgcttcggtc
     6481 tgatttttgg ctttgaccaa ttcgctgtcc gaaattccac ccgcggcgag ccggtcaatt
     6541 tgggtgtaaa tcgatttctc cacgtccgcc agttcgtgcc ccggctgcaa ttcggcctga
     6601 accgcgaaga gactggggtc gcgggcgtcg tcgtaaaacg cattgacgaa cgtgaccgct
     6661 ttctcttttt ctactaactc ttgatagagg cgcgaactct tccccccgga gaggatggtg
     6721 gctaaaacgg tcagggggta gctgtccttc tcccccactt tcggtgtatg ccacccgacc
     6781 agaagccgcg ggagattcga ctccttgtgg acaataatcc gtcgctcgcc caattgctcc
     6841 ggctccaccg cccgcatgtc ggggattgct ccccgccgct cgagggtccc gaacaattgt
     6901 cggacttttt ccagggtctt ctccgtattg acgtcgccca cgataactaa gatggcgttg
     6961 gctggctggt agaaggtgcg gtagtaggcc tccatatcag cgattgagag cttgtctaaa
     7021 tccgtaatcc agccgatcac cgggtactgg taggggtggg ctttgaacgc ggccgcctgg
     7081 aagtcctcga agagctgatc ccacggcccg tcgttgcccc gcttgagttc ttcgattacc
     7141 acctgttttt ccggattgaa ttcttcggga agaaattgat tattcaccat ccggtcggct
     7201 tcaatttcca aggcctcttc ccaccggtcg gaggcgaagt tgaagtagta ggcggtgtag
     7261 tcgttggagg tgaaggcgtt gttcgagccg ccgttcttga gcgtgatgag gtcgatgtcg
     7321 cccttcttca acttcttggt tcccttgaag agcatgtgct ccagaaagtg cgagacgccg
     7381 gttttgccga tttcttcgtt gcgcccgcct accttgtacc agaccatgca ggagaccacc
     7441 gggatgtcgt gcttctcctt cacgagcacc acgaggccgt tgtccaaaac ttgcttggtg
     7501 accccgaagt tcggctgcgc ctgagccgcc gtgaaaagca aaacccagag gatgaaccac
     7561 ccgaccatac tgcgcttcat caactacctc ccttttcctg cgtcccgagt gccggaaacg
     7621 ttccttccag ttggtttcag tatggcgtcg cctgctccgg ttgtcaaatt tccggcccta
     7681 ctgaacgcct aagtccgaca gcaggtgctc ccgtaccaga ccggctgagt caaacattca
     7741 gcccacagtc gtgaaaaaaa accgccgtca gctctgcaac cgacggcggt ttctcgtaac
     7801 gacttgaatt atttgatctg cccgatgatg tcgaacatcg gcaggtacat ggcaatcagg
     7861 atcccgccga tgaccgtgcc catcaccacg atgatggagg gctcgatgat ggaggtcagg
     7921 gccgccaccg ccgcatcaac ttcttcgtcg taaaagtccg cgatcttgcc caacatttca
     7981 tccagtccgc cggttttctc gccgaccgaa atcatctgcg tcaccatcgg cggaaagacc
     8041 cccgactccc gcagcggccc ggtgatggta tcgccctggg caatggccac caccgacttc
     8101 tttatggcgt catggatcac ccggttcccc gcggtcttgg ccgtggtctc cagggcgtcc
     8161 agaatggaaa caccggaaga aatcaaagtc cccaacgtcc gggtaaagcg ggcaatggcg
     8221 gtcttgcgga tgagcgttcc cagaagcggc agccggagtt tgaatttgtc gatcgccagc
     8281 cgcccgccgt cggtgcggcc ccagagcttg atgaaagcca cgagcgcgcc ggcgcccaga
     8341 atgataaaga agatgttccc gcgcaaaatg gaggagatct tgagcacgaa ctgggtcggg
     8401 ccgggcagtt ccgaccccag gccctggaac atcttggcga agatcggaac gattttcacg
     8461 agcatcaagg tggtgaccag aatggcgacc acgacgatca ccgccggata gaccatggcc
     8521 cctttcacct tgcgggtcag ggcgtcggct ttctcccggt aggcggccag ccggttcaaa
     8581 atcgagtcca aagcgccgcc cacctcaccg gcttccacca tgttgacgta caggtcgtcg
     8641 tacaccttct tgtgcttgcc catggcttcg cagagggtgc tgccggagga gacggagtct
     8701 ttaatttcgc cgatgacctg gcgcagctct ttggattcgc tctgtgctgc cagaatttct
     8761 aagcactgga ccatgggcag tccggcgctg atcatggtgg caaactgccg cgtgaagcgg
     8821 gagatgtgga tcttcttgat cccggtgccg aaggagagct taatttgcga cggcttgcgg
     8881 gacagtgagg agaccaggat ccggttggtg cggaggatcc gctccagttc ggcccggctc
     8941 ttggctttca gttctccgga aaccgcgact ccggcggcgg ttttcccttt gtactcaaag
     9001 accggcacgg tagccttcct ctcttaggcg taggcgagtt cctgccgccg cgccagcatt
     9061 tcggagagtt cctgcacgtt ggtgctgcgg ccgaaggcct cgccctgcgt gatcttgcgc
     9121 gtctgcacca gctccgacag cgattggttc atcgtcttca tcccgtactt ctgccccgcc
     9181 tggatcagac tgtagatctg gtggatcttg tcgtcacgaa ttagggcccg gatggccggc
     9241 gtgcagacca tgatctccag cgccagcacg cgccccccgc cgatcttggg aatcaactgc
     9301 tggcagagca ccgcctgcaa aacgaacgaa agagtcaccc gcacctgcgg ctgctggttg
     9361 ttggggaaga cgtcgatgat gcgcgtggtc gcctccatcg ccgagttggt gtgcagggtg
     9421 gcaaacgcca ggtgtccggt ctcggagatg ttgagtgcgg cttccaccgt ttccaggtcc
     9481 cgcatctcgc cgattaatac gacatccggg tcttcccgca gggcgtactt caaggccgac
     9541 gcgaacgact gggtatcggc gtggacttcc ctttggttga tcaggcacag gttgtgacgg
     9601 tgcaggtact caatcggatc ttccacggtg atgatgtgat cgtgccgttc ccggttgatt
     9661 ttgtcgatca aggccgccag ggtggtcgac ttgccagagc cggtggggcc gcagaccaag
     9721 accaacccct tggggaggtt ggcgaattca gacaccactt tgggaatacc cagctcttcg
     9781 aaggactgaa tccggaacgg gatttgccgg atcgccgccg ccacgttgcc ccgctgcatg
     9841 aagatgttgc accggaagcg cgacagctgt tcgatgccga aagagagatc cagttcgctt
     9901 ttttcctcga acttctgacg ctgcttttcg ttcatgatgc tgtaggccag tttcttggtc
     9961 atttccggag tgagcatctc caggtcggtc cgggccagct tgccgtcaat gcgcagcatg
    10021 ggcggggcac ccacggtcag gtgcagatcg gaagcattgc gtttgaccat caattccaga
    10081 agatcgcgca gcgaagtcat ccgggctcct tcaggttacg gtcaaaaaga gaacagtcgt
    10141 gcttttggta ttatcggcaa gtggcaggga ttcttaagga aatggcgccg acgggcggat
    10201 tccccggccc cgggttgggc cggcgtaaaa agggaccgct cggggaaggc cggcgtgccg
    10261 gccggtttct ctttaccggc ccaaaaagtg cgtggacctg cacgaattcc cgcatcgcgg
    10321 gcggagtaaa tgcattaagt cgagcggttc gtcggactgg ttggctgttg gagttcttct
    10381 ccccgcaatt cggacaccgg cgccccggca gtatttgcat agccggggag gatgcattac
    10441 tttttatcta tccgggcgac cggatttctt aatgcgaacg gtcaaaaaaa cgccggcggg
    10501 acagcaccgt tcatcccgaa tttggcggtg caaccgggaa gaaaatggtc aaagaacagg
    10561 ccgatttatc ctctctgcga attgagcgga gctccgacta ccggccgccg cggaagtggt
    10621 ggcgctgggt tctgggactt tcgatcgttc cgcttctggt gatcggctac ctcttcgtta
    10681 tgcaccaggt ggcgccgggg ctggccgtcc aggtgggaac cgcgaccttg atcaccggca
    10741 gtcaggcgca agcgcttttg accgcctcag gctacgtggt ggcccagcgc aaagcggcgg
    10801 tggcctccaa ggccaccggg cggctggagt acttgggcgt ggtggaaggc gaccggatca
    10861 aagcgggaat ggtcatcgcc cggctggaaa aggccgacat caccgccgaa ctcgaccgcg
    10921 ccaaagccaa tctggcccac gccgtggcgg aatcgaccga ggcgactttg ttttatgaaa
    10981 ggcagaaaaa aatggtggcc gattctttga tcagcaacct ggaatttgag ggtgccgagg
    11041 cgcgctacca gcgggcgctg gccggagtcg cctccggccg ggcggcggtg aaggcggcgg
    11101 aagtggccct ggagaacacc gtcatccggg cgccctttga cggaacggtt ctgaccaagt
    11161 cggcggaagt gggcgaaatg gtggcgccct ttgcttcgac cgcttcttcg cgcggggcgg
    11221 tggtgacact ggccgatatg aattcgctgg aagtcgaagc cgacgtggcc gaggcgaata
    11281 tcgagcgggt gcaggtggga ctgccctgcg aaattaccct ggacgcctac ccggataccc
    11341 gctacccggg gaaggtgtcg aaaattgtcc cgacggctga ccgcacgcgg gctaccgtgc
    11401 tggtcaaagt cgctttcctc tcccgtgacc ggcgggtgtt gccggaaatg agcgccaaag
    11461 ttaatttttt gccggagaat acccaacttt cggctcaaaa taccgagccg gtagtggccg
    11521 tgccaaactc ggcggtgacc atgcgcaacg gagtgaagat ggtttttctg gtagagggaa
    11581 atttggtcaa aagcgtgccg gtgaccaccg ggcgggttct gggagatatg accgagatta
    11641 aagccggtct gcaggccgcc gaccgggtgg tgatctcccc cccgggcagc ttagccaccg
    11701 gcatgaaggt gaaaacgtcg gagtagttca accgtagagg aacctatggc cgaatcaatc
    11761 gtcgaaatcg aagcgctttc caaatcgtac taccgggaca gtctggaaat tccggtgctg
    11821 cacaatttga atttttccat tcccgaaggg gagtttgtgg ctttaatggg gccgtccggc
    11881 tccggcaaga ccaccctttt gaatatgatt gccgggattg accgccccac gaagggcaac
    11941 gtcaaagtcg ccggcaccga cttaggccgg ctgggcgaag gggaattggc ccgctggcgg
    12001 gcgcggcaca tcggcttcgt ctttcagttt tataacctga tgccggtcct gaccgcgtat
    12061 gaaaacgtcg agttgcccct cctgctgacc aatctgtcga aaaaagaacg tcggaagcac
    12121 gtcgaaacga cgcttggcat tgtggggctg gcggaccgga tgaagcacta cccc
//
LOCUS       DBNGZD010000039         3546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1325570, whole genome shotgun sequence.
ACCESSION   DBNGZD010000039 DBNGZD010000000
VERSION     DBNGZD010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1325570"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1872
                     /locus_tag="AC16GM_01165"
     CDS             262..1872
                     /locus_tag="AC16GM_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2922316.1"
                     /translation="MLRFVVSVVFLCLFLPFSSTSGQVTQLYQINGAAGDSLGYSVAG
                     VGDVNGDGKADFIVGAPAAGGVGSAYIYSGVTGVLLFQKNGAAADDNFGNSVAGTGDV
                     NGDGKDDFIIGAPFADPGGLTNAGSTFVYSGATGLLLFQKDGAAADDRLSWSVAGAGD
                     VNGDGKADFIIGAWAADPGGLPYAGSAFVYSGADGSLLFQKNGVAAGDLLGASVARAG
                     DVNGDGKADFIVGAPFADPGGLSMAGSAFIYSGTDGSLLFQKDGSATGDAFGISTAGA
                     GDINGDGKSDFIVGANFADPGGVGYAGSVFVYSGADGSLLFQKNGTAFFDNLGNSVAG
                     AGDVNGDGKADFIVGAYRADGGGLVDAGSAFVYSGADGSLLFQINGVTANDNLGFSVA
                     GVGDMNGNGRADVIVGARLADPGGLTDAGSAYVYSFSPTGVTKEEFAGIPSSFLLAQN
                     FPNPFNAGTQIVFELPGAESRSVRLEVFDILGQRIATLIDQSLSGGRYHVTWDGKDGQ
                     GKAVGSGVYFYRLTAGEFKETRRMTLIK"
     gene            2156..3319
                     /locus_tag="AC16GM_01170"
     CDS             2156..3319
                     /locus_tag="AC16GM_01170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR2922317.1"
                     /translation="MIKEKIKQAIAILNEKKIDLWLTFVRETGTFADPAMDLVGGAEV
                     VWQSAFLISASGESIAIMGTYDTENFKRQGNYQKIIGYDQDITPVLLTEIKRLDPVTI
                     AVNYSPDNDLADGLTHGMYLLLDRIFAGTPYRNRLISAEPVLSALRGRKLPVEVTRIK
                     ESIRTSDEIFKQLLGFLKPGLTEKQVDEFVTSQFEAHKVKRAFPTIINHGAKSPVGHV
                     GPSDAVLEKGDLFHIDFGVKKDGYCSDIQRMVYFLAPGEKKPPTNVQKAFDAVRKTIT
                     TAAEHLKPGAIGYKIDAVGRQTVTACGYPEYNHALGHQLGRAVHDGGTLLGPRWPRYG
                     QATEGEVEAGNVFTLELGANVPGKGTVSLEEDVLVTERGCEFLTKPQAELICL"
BASE COUNT          745 a          910 c         1072 g          819 t
ORIGIN      
        1 aacgtggccg ggttgcgaaa atcaagtgtg caggccggtc ggcaaacaaa taggagaata
       61 ctttcttcca ccggccaaag gggacgacag ggggttagaa aggctgagac taaactcgtt
      121 gtggcccatc ccggactgag accctttcca cacgagagga tttgttgaca ttccagcccg
      181 gctcggttta ttaactctta gagttttgcg caaggccggg tgaatacgag acaaatggtc
      241 tcgtctgcac gggaagaagg tatgttgcgc tttgtagtga gtgttgtttt cctctgcctg
      301 ttcctccctt tttcctcaac ttctggacaa gttacgcagc tttaccagat aaacggcgcc
      361 gccggtgaca gtcttggcta ctccgtggcc ggggtgggag atgtgaacgg cgatggaaaa
      421 gctgatttta tcgttggcgc tcccgctgcg ggcggcgtag gctccgccta tatctattcc
      481 ggtgtcaccg gtgttctcct tttccagaaa aacggggccg ccgccgatga caattttggt
      541 aactcagtcg caggtactgg ggatgtaaat ggggatggaa aagacgattt cataattggc
      601 gctccttttg ccgacccggg cggacttacc aatgccggct ccacttttgt ctattccggc
      661 gccaccggtt tactcctttt ccaaaaagac ggcgccgccg ccgatgaccg gctgagctgg
      721 tccgtggccg gggccggaga cgtgaacggg gacggaaagg ctgattttat catcggcgct
      781 tgggctgccg acccgggcgg acttccgtat gctggctcgg cttttgtcta ttcgggagcc
      841 gacggctccc ttcttttcca gaaaaacgga gtagctgcgg gcgatctgct gggtgcctcc
      901 gtcgccaggg ctggtgatgt gaacggggat ggaaaagccg attttatcgt cggtgctcct
      961 tttgccgacc cgggcgggct ttccatggcc ggttcggcct ttatctattc tggaactgac
     1021 ggctcccttc ttttccaaaa agatggctcc gcgaccggtg atgcattcgg tatctcgact
     1081 gctggagccg gggatataaa tggagatgga aaatccgatt tcattgttgg cgctaatttc
     1141 gccgacccgg gcggagttgg gtatgcgggc tcggtttttg tctattctgg agccgatggt
     1201 tctctcctgt tccagaaaaa cggcaccgcc ttctttgaca atttgggtaa ttccgttgct
     1261 ggagccgggg atgttaacgg agatggaaaa gctgatttta ttgttggtgc atatcgtgcc
     1321 gacgggggtg gacttgtcga tgctggctcg gcttttgtct attccggtgc cgacggctcc
     1381 ttgctttttc agataaacgg cgttactgct aacgacaact taggtttttc ggtggccggg
     1441 gtcggggata tgaatgggaa tgggagagct gatgtaatcg tcggcgctcg tcttgctgac
     1501 ccgggcgggt taaccgacgc cggttcggct tatgtttatt ctttttcccc caccggcgtg
     1561 accaaagagg agtttgccgg aattccctca agtttcttgc tcgcgcagaa tttccccaat
     1621 ccgttcaatg cgggcaccca gattgtcttt gaattgcccg gagcggaatc gcgctcggta
     1681 cggctggagg tatttgacat tctggggcag agaattgcga cgctcattga tcaatctctc
     1741 tctggcggga ggtaccacgt tacctgggac gggaaagacg ggcaggggaa ggcggtggga
     1801 agcggggttt acttttaccg cttgacggcg ggggaattca aggaaacccg gcggatgacg
     1861 ttaatcaaat aatttcacag cagatgaagc agatggagcg gattaatttt gtaggggcga
     1921 atctttactt gtcctgagcg aagccgaagg agattcgccc aagaataagc gggtgaatcc
     1981 gccttaggcg tattcactcc tacatttttt gagaattttt tggcggaggt gaacctccgc
     2041 attatatttc cgtaaaatga tttgacccct ccctgccctc ctcggtacga gtctatcgac
     2101 cctccgaggg gagggaaaaa aagaggggtt aaggaaacct ctcagaaagg gcaaaatgat
     2161 caaggagaag atcaaacaag cgatagcgat cctcaacgaa aagaaaattg atctctggct
     2221 caccttcgtg cgggaaacgg gcacgttcgc cgatccggcg atggatttag tcggcggggc
     2281 ggaggtggtc tggcagtcgg cgtttctcat ttccgcctcg ggcgagagca tcgccattat
     2341 ggggacctac gacaccgaaa atttcaagcg ccagggaaat taccagaaaa tcatcggcta
     2401 cgatcaggac attacaccgg tgcttctaac tgaaatcaag cggctggatc cggtcaccat
     2461 cgcggtgaac tactcgcccg acaacgacct cgccgacggc ctgacgcacg ggatgtacct
     2521 gttgctcgac cggattttcg ccggaacgcc gtaccggaac cgactgattt ccgcggagcc
     2581 ggtgctttcc gctctgcgcg gccggaagtt gcctgtggaa gtaacgcgga ttaaggaatc
     2641 aatccgcacg tcggacgaaa ttttcaagca acttttgggt ttcctcaagc cggggctgac
     2701 ggagaagcag gtggatgagt tcgtcaccag ccagttcgaa gcgcacaagg tcaagcgcgc
     2761 ctttcccacc atcatcaatc acggggcgaa gtcgccggtg gggcacgtgg ggccgtcgga
     2821 tgccgtcctg gaaaagggcg acctgttcca catcgatttc ggcgtcaaaa aagacggcta
     2881 ctgctccgac atccagcgga tggtctactt cctcgcgccg ggggagaaga agccgccgac
     2941 aaacgtgcag aaggcgtttg atgccgtccg caagacgatc accaccgcgg cggaacacct
     3001 caaaccgggg gcaatcggtt acaagatcga tgcggtcggg cggcagacgg tcaccgcgtg
     3061 cggctatccc gaatacaatc acgcgctggg acaccagttg ggccgggcgg tccacgacgg
     3121 cggaacgctt ttggggccgc gctggccgcg ctacgggcaa gcgacggagg gagaagtgga
     3181 ggcgggaaac gtcttcacat tggagttggg agcgaacgtt cccggcaagg ggacagtttc
     3241 tctcgaagaa gacgttttag tgacggagcg ggggtgcgag tttttgacca agccacaggc
     3301 agagttgatt tgtttgtaag tttttgaccc ctccccagcc ctcccctcta aggggaggga
     3361 agatagatct tcttaccctc tgggcatagg cgggtcggaa cgactcgtac cgagactctc
     3421 aatcccgccg aatttcctac ttcccgcgta gggcgacccc tacggaccga gttgcaaccg
     3481 ggttataacc aggccgcccc ggtcgcccct ccattttagg gattgggctg ttttaaattc
     3541 gtttga
//
LOCUS       DBNGZD010000040         3082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1672926, whole genome shotgun sequence.
ACCESSION   DBNGZD010000040 DBNGZD010000000
VERSION     DBNGZD010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1672926"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="AC16GM_01175"
     CDS             complement(<1..893)
                     /locus_tag="AC16GM_01175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2922318.1"
                     /translation="MDFDSDLKIPGHDFYLDAPRAVPCSFVSHAHSDHVRRHGKMVAT
                     PETILLCRLRFKSASGGRARAVLFGQPTKIDDLKIELFPAGHMLGAAQILVQLDDQKI
                     VYTGDFNLQPGYTCPGAEVKPCDVLIMESTYGRPHYRFPEREKVAQQICAFIDAAFAS
                     RKIPVLLAYAVGKAQEVMKLLGDRGYKMAVHKSIFDVARVYQRCGVELTNYDLFSDPL
                     LPETVLIFPPFFWKLPPVAKIENRRVAFLSGWGIDPQAKQLYHADEVFPFSDHADFAD
                     LVAYVQKANPQKIYTLHGFSE"
     gene            complement(1020..1367)
                     /locus_tag="AC16GM_01180"
     CDS             complement(1020..1367)
                     /locus_tag="AC16GM_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2922319.1"
                     /translation="MAGFRDLKVWQKAFTLSVEAYRITKNFPAIEQYALSSQLTRAAV
                     SVPANIAEGYDRTSKKEYRYYVSIAKGSLAEVETYFLLAMELGYITREDFNKIDSLRA
                     EVGRMLTGLLKSI"
     gene            complement(1443..1925)
                     /locus_tag="AC16GM_01185"
     CDS             complement(1443..1925)
                     /locus_tag="AC16GM_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HIR2922320.1"
                     /translation="MELKTVRLAMPKEANVIFGQTHFIKTVEDLYEILVGMLPGIKFG
                     LAFCEASGPCLVRAEGNDGELKAAAIAGAQQVASGHTFVIFLRQAFPINVLNAIKNCS
                     EVCSIFCATANPVEVVIAQTEQGRGVLGVIDGFPPKGVESDDDLTRRREFLRKMGYKK
                     "
     gene            complement(1945..>3082)
                     /locus_tag="AC16GM_01190"
     CDS             complement(1945..>3082)
                     /locus_tag="AC16GM_01190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2922321.1"
                     /translation="PYLVGEELTALGSVHSDSAYQVVRAFLASPIPDLRARATAALAQ
                     IDSARALEDLKPLRSDTNWHIRAQAAEAFGAIRTPEAFALLESLFVDPDYRVRYPALE
                     AMGKFPTERWEPFVKRALGEDEQALIATASEIAGKAHDSTLLNDLVVGFWKHRMDRET
                     DAKIAILEALERFVDAGRLSGTIAQALDFGLQDTNRLVRQLAVKIFRKAGLERGGFTG
                     TFVSDMDAEKYRAVYEQYTTNPKAAITTGKGIIVLELLPREAPRTVANFIKLAQTGFY
                     DNRVWHRVVPNFVIQDGCPRGDGWGGPGYTIRCEYNKLHYERGTVGMALSGKDTGGSQ
                     YFITQSPQPHLDGRYTIFARVVEGMEVVDKIMVGDKIEKVTIIE"
BASE COUNT          615 a          896 c          915 g          656 t
ORIGIN      
        1 aattcggaaa aaccgtgcag agtgtaaatt ttctgggggt tcgccttttg aacgtaagcg
       61 acgaggtcgg cgaaatcagc atggtcggag aaagggaaaa cttcgtcggc atgatagagt
      121 tgtttcgcct gggggtcaat gccccagccg gagaggaagg cgacgcgccg gttttcgatt
      181 tttgccaccg gcggcagttt ccagaaaaac gggggaaaaa tgagaacggt ctcaggtaac
      241 agcggatcgg aaaagagatc atagttcgtc agttccaccc cgcagcgctg gtaaactcgg
      301 gccacgtcaa aaattgactt gtggactgcc atcttgtagc cccggtcgcc cagaagtttc
      361 atcacctcct gcgccttgcc caccgcatac gccagaagca ccggaatttt ccggctggcg
      421 aaggcggcat cgatgaaagc gcaaatctgc tgcgccacct tctcccgctc cggaaagcgg
      481 tagtggggcc ggccgtaggt tgattccatg attaaaacgt cgcagggctt gacctccgcg
      541 ccggggcagg tgtagcccgg ctggagattg aaatcaccgg tgtagacgat tttttgatcg
      601 tcgagctgga cgagaatctg tgccgctccc agcatatgtc ccgccgggaa gagttcgatt
      661 ttaagatcgt cgattttggt cggctggccg aagagcaccg cccgggcccg tccgcccgag
      721 gcggacttga accggagccg gcagagcagg atcgtctccg gcgtggcgac cattttcccg
      781 tgccggcgga cgtggtcgga gtgggcgtgg gagacaaaag agcacggcac cgcgcgcggc
      841 gcgtctaagt agaagtcgtg gccggggatc ttgaggtcgc tgtcaaaatc caaaggaagc
      901 gtccagtagc cagataacag tagccagggg ccagtagcca gtagccagaa aaataaaaaa
      961 gcccgggggc cagcacgtct tgatagacgg ctcacgaaat agttcttttc ccagaaacct
     1021 catatgcttt tcagcagccc tgtgagcatg cgcccgacct cggcgcggag tgaatcgatt
     1081 ttattgaaat cctcgcgtgt aatgtaacct aattccattg ccagaagaaa atatgtttcg
     1141 acttcggcca gagagccttt ggcaatggaa acgtaatacc tgtattcttt tttgctcgtt
     1201 cggtcatacc cttcggcgat attggccggc acggaaaccg cagctcgggt aagttggctg
     1261 gaaagcgcat attgctctat ggcagggaag tttttggtta ttcggtaggc ttcaaccgac
     1321 agggtgaacg ctttttgcca caccttcaaa tctcgaaacc ctgccattgg tttaactggc
     1381 tgctggcccc tggctactgg ttttttctct ctggtgactg gctactggcc cctggctact
     1441 ggctactttt tatagcccat cttacgcaaa aactccctcc ggcgcgtcag gtcatcgtcg
     1501 ctctcgaccc ctttgggcgg gaagccgtcg atcaccccca gcaccccccg cccctgttcg
     1561 gtctgggcta tgacgacctc caccggattg gcggtggcgc agaaaattga gcaaacttcg
     1621 gagcagtttt tgatggcatt gagcacgtta atggggaaag cctgccgcag gaagatgaca
     1681 aaggtgtggc cggaggccac ctgctgggcg ccggcgatgg cagcagcctt aagttcgccg
     1741 tcgttgccct ccgcccgcac caaacagggc ccggaagctt cacagaaggc taagccgaat
     1801 ttgatcccgg ggagcatccc caccaggatt tcatagagat cttcgacggt tttgatgaag
     1861 tgggtctggc cgaaaatgac attcgcctct ttaggcatgg ccagccgcac cgttttcaat
     1921 tccattgtgc tctcctcact acggttattc gatgatggtg accttctcta ttttgtcgcc
     1981 gaccatgatt ttgtccacga cctccatccc ttcaaccacg cgcgcgaaaa tggtgtagcg
     2041 gccgtccaag tggggctgcg gcgactgggt gatgaagtac tggctgccgc cggtgtcctt
     2101 gccggagagc gccatcccca ccgtcccccg ctcgtagtgg agtttgttgt actcgcagcg
     2161 gatggtgtac cccggcccgc cccagccgtc cccgcgcggg cagccgtcct gaatgacgaa
     2221 gttcggaacg acccggtgcc agacgcggtt gtcgtaaaaa cctgtctgcg ccagtttgat
     2281 gaaattcgcc accgtgcggg gcgcctcccg gggcaaaagt tccaggacaa ttatcccttt
     2341 tccggtggtg attgccgctt tcggattggt cgtgtactgc tcgtacacgg cgcgatattt
     2401 ctccgcatcc atatcgctga caaacgttcc ggtgaagccc ccccgttcca gaccggcttt
     2461 ccggaagatt ttcaccgcca gctgccgcac caggcggttg gtgtcctgca ggccgaagtc
     2521 gagcgcttgg gcgatggtgc cggataggcg gccggcatcg acgaaccgct ccagggcctc
     2581 gaggatggcg atcttggcgt cggtctcccg atccatccgg tgcttccaga accccacgac
     2641 gaggtcgttc agcagggtcg aatcgtgcgc cttgcccgcg atctcgcttg cggtggcgat
     2701 gagtgcctgc tcatcttccc ccagggcccg tttgacaaac ggctcccagc gttcagtggg
     2761 gaacttcccc atcgcctcca gcgccgggta gcggacgcgg tagtcgggat cgacgaagag
     2821 cgactctaag agcgcaaagg cctccggcgt gcggatggcg ccgaacgctt ccgccgcttg
     2881 cgcgcggata tgccagttgg tgtcagatcg taacggcttg aggtcttcca gcgcgcgggc
     2941 cgaatcgatc tgcgccaaag ccgccgtcgc gcgcgcccgg aggtccggaa tcggactggc
     3001 gaggaaggcc cgtaccacct ggtaggcact gtcactgtga acactcccca gtgcggtcag
     3061 ctcttccccg accaggtagg ga
//
LOCUS       DBNGZD010000041         7682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1881262, whole genome shotgun sequence.
ACCESSION   DBNGZD010000041 DBNGZD010000000
VERSION     DBNGZD010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1881262"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="AC16GM_01195"
     CDS             <1..841
                     /locus_tag="AC16GM_01195"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2922322.1"
                     /translation="GTIYGLLGPNGAGKTTTIRMLLNIIAPDTGTVRINGQLWSEELK
                     NKIGYLPEERGLYRKMKVADLLLFLAEIKGVPRAQAKQRMTPWLARLDLEKWKERKAE
                     ELSKGMQQKLQFIATVLHEPELVVLDEPFSGLDPVNTDILKDIVLELRQKGTTVLFST
                     HVMEQAEKLCDAICLINRGQKVLEGSLGQIKSRFGRNSVRVAVEGDGQFLRQLPGVQK
                     IDDYTNYAELQLSVGAAPQAILQTIAQRATVKRFEVVEPSLHQIFLEVVRDHPTEKTG
                     EKP"
     gene            838..2103
                     /locus_tag="AC16GM_01200"
     CDS             838..2103
                     /locus_tag="AC16GM_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2922323.1"
                     /translation="MKDLWAVLKREFTEATRKKSFLIWTIITPFYMALLFALPSWLAS
                     KKSGPDRPKQIAIIDRTGILRDRLIAALVDTLPNGRKMFQVETLDPQLSPDSTEALCR
                     ARVLEGTLDAYVNIPAEALDSGRAQFYSKSTGDFILPNAIERRLNQALLQYRSRTFGI
                     DSTVAAALTRRADLAALKVTKEGAKTTQFITGYLVALSFVMILMSLILSFGQTLMRGI
                     IEEKNSRVIEVLVSSVRPFQLLMGKVLGLGVAGLFQIVIWVAMAVLAFALTGSQGWLG
                     QALTSIPGNLVTLFIIYFILGYFLYSAFFALIGAVCQSEQEAQHLMFPLILFLIMPFI
                     LGFMVSQNPGGSVSTLLSFFPLLTPTLMVMRSYYLMPPVTQIAASIVVLLVSIVLVGW
                     FSAKVFRVGILMYGKRPTLPEVLRWVRYR"
     gene            complement(2176..2781)
                     /locus_tag="AC16GM_01205"
     CDS             complement(2176..2781)
                     /locus_tag="AC16GM_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR2922324.1"
                     /translation="MRTAIFIDGAYLEFTLRESYESVRIDYGLLSEKLAGGIDVLRSY
                     YYNCLPYVGNPPTDEEKTRLADKQRFFDALTRIPRYQVRLGQLAFRGLRTGDNRPIFE
                     QKRVDTLMAVDFVLLCSTHRITHAALLTGDGDFVPAVNVAKEFGVVVSLWYSDRFRAD
                     DELWRVCDERHVIDQQLIDSIRLRSYANGETVRAGRSDSSF"
     gene            complement(2861..2942)
                     /locus_tag="AC16GM_01210"
     tRNA            complement(2861..2942)
                     /locus_tag="AC16GM_01210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2906..2908),aa:Leu,seq:gag)
     gene            complement(3004..3402)
                     /gene="secG"
                     /locus_tag="AC16GM_01215"
     CDS             complement(3004..3402)
                     /gene="secG"
                     /locus_tag="AC16GM_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR2922325.1"
                     /translation="MFTFFLVLHILVCIGLIISILMQSAKGEGLAGAFGGGSISSAVF
                     GGRGAATFLARATTVLAVAFGLTCVSLTLTAPRAGTSGTSAVAKEAAKGGIPAPQPPP
                     PTQVPVTQQPAQPAGQQQPDVNQPPAPKKK"
     gene            complement(3412..4173)
                     /gene="tpiA"
                     /locus_tag="AC16GM_01220"
     CDS             complement(3412..4173)
                     /gene="tpiA"
                     /locus_tag="AC16GM_01220"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR2922326.1"
                     /translation="MAKPSIIAGNWKMHKTDAEAQALALALRKSIPSGSAQVVLCPPF
                     IALAGVAQAISGSALHLGAQNLYPAKEGAFTGEISGAMLIALGCTYVIIGHSERRHIL
                     GEDDAFINRKLKAAGEVGLIPIFCVGETLDQRQAGKAFDAVWTQLEQGLADIPGEAIQ
                     KMVIAYEPVWAIGTGQVATLQQAVEVHRFIRDRLSAHRIDGQAPSILYGGSVNPANAA
                     DLLRETEIDGLLVGGASLEAQAFSHIVAAAQVKTR"
     gene            complement(5218..6411)
                     /locus_tag="AC16GM_01225"
     CDS             complement(5218..6411)
                     /locus_tag="AC16GM_01225"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR2922327.1"
                     /translation="MAKLSIDDLKLRGKRVLVRVDFNVPLENGRVADDIRIVASLPTI
                     KKILADGGRAILCSHLGRPKGKPTPEFSLLPVARRLEELLGKKVAFATDCVGPVAEQA
                     VANLRDSECLLLENLRFHSEEEKNDPAFSAQLAKLGDLYVNDAFGSAHRAHASTEGVA
                     RLFQQRAAGYLMQKELHYLGKATANPKRPFIAILGGAKVSDKIAASENLLSKVDALLI
                     GGGMAFTFLKAQGKEIGGSLVETEKIETAKMLLQKAKEKKVDLVLPVDCVVAEKIEVN
                     VPTAVVPVDRILPNQKGLDIGSRTVELFANKLSTAETVIWNGPLGVFEINAFAQGTFA
                     IAKTLAQLTQKGTVTIIGGGDSAAAVAKAGLANLMSHISTGGGASLEFLEGKELPGVV
                     VLTDK"
     gene            complement(6448..7140)
                     /locus_tag="AC16GM_01230"
     CDS             complement(6448..7140)
                     /locus_tag="AC16GM_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922328.1"
                     /translation="MQPLRFLFGKTHFILDFSIPLALLLHAIPAAAEENREKPRFNQL
                     SGRHPVVSVPMVQTIGPLRFSIARLEAARLAYPNFGNQKRDQHFFVSNSDGEHGERPQ
                     PSIAQPIGIGLFFAAIGFFAGAGIGAGILGGSDDGEFSRFEGAIIGAPIGEATFMPVG
                     VHLGNNRQGFFPIVFLNSLVVGGVGIWAASATDKDIHPAITLSATVIAQLLTCVSIER
                     AGSRHRRSNLRE"
     gene            complement(7170..>7682)
                     /locus_tag="AC16GM_01235"
     CDS             complement(7170..>7682)
                     /locus_tag="AC16GM_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR2922329.1"
                     /translation="HAYTNDQRILDQPHKDLRRARAAAYSIIPTTTGAAKAIGQVIPE
                     LEGKLDGVAVRVPISDGSLLDLTCELEKDVTAASVNQAMKEAAEGGMKGILQYCEDPI
                     VSADVVGNPYSAVFDAQATMVIGKNLVKVFAWYDNEWAFSVRMVETIQIMMAGAVGDK
                     TERIATVAAR"
BASE COUNT         1631 a         2264 c         2042 g         1745 t
ORIGIN      
        1 agggacgatt tacggcctct taggccccaa cggcgccggc aaaaccacca ccatccggat
       61 gcttttaaac atcattgcgc ccgacaccgg cacggtaaga atcaacggac aactctggtc
      121 ggaggaattg aagaataaaa tcggctacct gccggaagag cggggactct accggaagat
      181 gaaagtggct gatcttctct tattcctggc cgaaatcaaa ggtgtgccgc gggctcaagc
      241 gaagcaacga atgacgccct ggctggcgcg cctcgactta gagaaatgga aagagcgcaa
      301 ggccgaagaa ctctccaagg ggatgcagca gaaactccag ttcatcgcca cggtattgca
      361 cgaaccggaa ttagtggtgt tagacgaacc cttctccggt ttagacccgg tcaataccga
      421 tattctgaaa gacattgtcc tcgaactgcg ccagaagggt accacggtcc ttttctctac
      481 ccatgtgatg gagcaggcgg agaaactctg cgatgctatc tgcctcatca atcgcggcca
      541 gaaagtgttg gaaggctccc tggggcagat taaatcgcgc ttcggacgca atagcgtccg
      601 cgtggccgtt gagggcgacg gccagttcct gcggcagttg cccggggtgc agaagatcga
      661 tgactacacc aactacgccg aactgcaact ctccgtcggc gccgcaccgc aagctatcct
      721 gcagacgatt gcccagcggg ctacggtaaa acgcttcgag gtggtggaac catccctgca
      781 ccagattttc ctcgaagtgg tacgcgacca tcccacggaa aagacgggag aaaagccgtg
      841 aaagacctgt gggcagtgct caagcgggag tttacggaag ccacgcgcaa gaagagtttt
      901 ctcatctgga cgattatcac tcccttctac atggccctgc tctttgccct gccgtcgtgg
      961 ctggcatcca agaaatcggg ccccgaccga cccaagcaga ttgccatcat tgaccgcacc
     1021 ggcatcctcc gtgaccgcct gattgccgct ttagttgaca ccctccccaa cggccggaaa
     1081 atgttccagg tcgaaacgct tgatccccag ctttccccgg acagcacgga ggcactttgc
     1141 cgcgcacgcg tgctggaggg cacgttggac gcttacgtta acattccggc cgaggccctc
     1201 gactccggcc gggcccagtt ctattccaaa tccaccggcg acttcattct gcctaacgca
     1261 atcgaacgac gcctgaatca ggcgctcctg cagtaccgct cgcgcacctt cgggatcgat
     1321 tccaccgttg cggccgcttt gacccgccgc gccgacctcg ctgcgctgaa agtcaccaag
     1381 gaaggtgcta aaacgacgca attcattacc ggctacctgg tggctctttc cttcgtgatg
     1441 atcctcatgt ctcttatttt gagtttcggg cagaccttga tgcggggcat cattgaggag
     1501 aagaactcaa gggtgatcga agtcctggtc tcctccgtcc ggccgtttca gctactcatg
     1561 gggaaagtcc tggggctggg ggtggctggt cttttccaga ttgtcatctg ggtggcgatg
     1621 gcggttttgg cttttgctct gactggctcc cagggctggc tggggcaggc cctcacctca
     1681 atccccggga acctggtgac actctttatt atctatttca ttctcgggta ttttctctac
     1741 tccgccttct tcgccctcat cggagcggtc tgccagagcg agcaggaagc ccagcacctg
     1801 atgttccctt taatactgtt cttgatcatg ccgttcattc tgggctttat ggtttcccag
     1861 aatccgggcg gctcggtctc gactttgctt tctttcttcc cattgcttac tcccaccctg
     1921 atggtgatgc ggtcgtacta tcttatgcca ccggtgacgc agattgccgc cagcattgtc
     1981 gtgctcctgg tctcaattgt tttggtcggc tggttttccg ccaaagtctt ccgcgtgggg
     2041 attctaatgt acggcaagcg cccgaccttg ccggaagtgc tgcgctgggt gcgctaccgg
     2101 tagagctatt tatcctgaag aaactaactt gctaacttga tggtagtccg gttcgtccct
     2161 aagccggacg aaccgtcaga acgaagaatc actgcgcccg gcccgaacgg tctctccgtt
     2221 cgcgtaagac ctcaagcgga tcgaatcgat caactgctgg tcgatcacgt ggcgctcgtc
     2281 gcagacgcgc cacagttcgt cgtcggcgcg gaagcggtcg ctgtaccaga gcgacaccac
     2341 cacgccgaac tctttggcca cgttgaccgc cggaacgaaa tcgccgtcgc cggtgagcaa
     2401 ggccgcgtga gtgattcggt gggtggagca gagcagaacg aaatcgaccg ccatcagcgt
     2461 atcgacccgc ttctgctcga agatcggccg gttgtccccg gtgcgtaatc cccggaaggc
     2521 cagctgcccc agccgcacct ggtaacgcgg aatccgtgtc agggcatcaa agaagcgctg
     2581 cttgtcggct aagcgcgtct tttcctcgtc ggtcggcgga ttgcccacgt agggcagaca
     2641 gttgtagtag taggagcgca ggacgtcgat gcccccggcc aacttttcgg agagcaggcc
     2701 gtaatcaatg cgcaccgact cgtagctttc ccgcagggtg aattcgaggt aggcgccgtc
     2761 aatgaaaatc gcagttcgca tagaccacta ctccttaaag tgtcagtttc gttctgtgca
     2821 acctcaatcg gtctcgtttt ctgcctggac gggcaaacta tgccgaaggc gggattcgaa
     2881 cccgcacgcc ttgcggcgcc accccctcaa gatggtgtgt ctgccaattc caccacttcg
     2941 gcatttatct gatggaagat aaagcaatgt tcccgggcgt ttcaccccac agaaccgctt
     3001 ttttcacttc ttcttcggcg ccgggggctg attcacgtcg ggttgttgtt gacccgccgg
     3061 ctgggccggt tgctgcgtaa cgggaacctg cgtcggcggt ggcggctgtg gcgccgggat
     3121 gccgcctttg gccgcttctt tggcgaccgc actggtcccc gaggtaccgg ctcggggggc
     3181 ggtcaaagtc aaggagacgc aggtgagccc gaaggccacc gccaatacgg tcgtggcgcg
     3241 ggcgagaaag gtggccgcgc cccgcccgcc gaatacggcg ctggagatac tcccgccgcc
     3301 gaacgcaccc gccagccctt cccctttggc cgactgcatc aaaatcgaaa tgattagacc
     3361 aatacaaacc agaatgtgaa gtactaagaa aaacgtgaac acaacttcct cctacctcgt
     3421 tttcacctgc gccgccgcca caatatggct gaacgcctgc gcctccagac tggcgccgcc
     3481 caccaataaa ccgtcaattt cggtttcgcg caacaggtcg gcggcgttcg cgggattgac
     3541 cgacccgccg tagaggatcg atggtgcctg gccgtcgatc ctgtgggcgg agagccggtc
     3601 tcgtataaaa cggtgtactt ccaccgcctg ttggagagtg gccacctgac cggtgccgat
     3661 ggcccagacc ggttcgtagg caatgaccat tttctgaata gcttctccgg gaatgtcggc
     3721 taatccctgc tccaattgcg tccacacggc gtcgaacgcc tttcccgcct gtcgctggtc
     3781 gagcgtctcc cccacgcaga aaatgggtat gaggccgacc tcccccgccg cttttagttt
     3841 acggttgatg aacgcatcat cctcacccag aatatgccgc cgctcggagt ggccgattat
     3901 gacgtacgtg caaccgagcg cgatgagcat cgctccggat atttcgccgg tgaacgcccc
     3961 ctctttggcc ggatagagat tctgcgcacc caggtgcaag gccgaaccag aaattgcttg
     4021 agcgacgccg gctaaggcga taaagggtgg acagaggact acctgtgctg atccagacgg
     4081 aatagatttt cgcaacgcca aggccagcgc ctgcgcttcg gcatcggttt tgtgcatctt
     4141 ccagttgccg gcgatgatgg atggcttagc cacgagaggg tactgatgtt tggctatctg
     4201 ataagtttgc tactcgactg cgaatttttg gtacggcaca aaggctgagg ctcgggctga
     4261 atactttacc gcaacttcat ttcaatcggg cgtgataccg cagttgttgc acacaacctg
     4321 ctgggaacag acgcgaccat tacaggacga gtagcaagtt atgtgctcac accagtaaat
     4381 acattctccg ttcgcccatc ccttgtcaca ctgacaaacg ctgagacagt tgttgcagta
     4441 gcagggtcct gccgccgctg gcaccttggg tgccattaca attcctacgg taaatgaaat
     4501 gaggagaact gcgacgaacg caaccaaaac ttttctcatg gcactctcct tccgtggttt
     4561 gtaccgagtt cagcccttgt gccgtcgtac cggtgtgttc ataactgaat ttcccggtct
     4621 tgtcaaatga attccatggt tttaaatgag aataagaggt gaagttttat ttttttcgtc
     4681 agtaccacat cccccgtagt tcttttcctt acagaaactc cagcgccgcc ccgtcgctca
     4741 tggagactat gtgtgctaaa gtccaaaaac ttggtacggc acaaaggctg aagaatcggt
     4801 cttccttaca cttctatcct accggcaaac cggtctgcaa aagctagtgc acgaacccga
     4861 gcccaggata cagcgagccc cgctgttgct acaagttccg tactggacgg gtttacggag
     4921 cccaggagta ctgcaatcca cacaggtggt acagtcgtca cttatacagg tgccgccgat
     4981 gccacattgg ggcgctgcaa tggacaacgc aggcaccaga accaatccta ccgcaaaaga
     5041 gaccagcagg actacggcta agacaatgat tggctttttc atgacatcct ccttccctgg
     5101 ttttaaccga cctcagcctt tgtgcttctg taccggctat tttcatagcc gatattgtca
     5161 tccttgtcaa gtacttttcc tgacccgcgg gaatcctcag acgagaaagc ccaaaatcta
     5221 tttgtccgtc agaaccacca ctcccggcag ttctttcccc tccaaaaatt ccagcgatgc
     5281 cccgccgccg gtggagatgt ggctcatcag gtttgccaat ccggctttgg ccactgctgc
     5341 cgcagaatct ccgccgccga tgatggtaac cgtccctttt tgcgtcagct gagcgagcgt
     5401 tttggcaatg gcaaaagttc cctgcgcaaa ggcgttgatt tcaaacacgc ccagaggccc
     5461 gttccagata accgtctcag cggtgctcaa tttgtttgcg aacagttcaa cagtgcggga
     5521 gccgatgtct aatccctttt ggtttggtag aattcggtca accgggacga ctgcggtggg
     5581 aacattcact tcaatttttt ccgccaccac gcaatccacc ggcaggacga gatcgacctt
     5641 tttctctttc gctttttgga gtaacatttt ggctgtctcg attttttccg tttccaccag
     5701 cgagccgccg atctccttcc cctgcgcttt gaggaacgtg aaggccatcc cgccgccaat
     5761 aagcagggcg tcaactttcg aaagcagatt ctcactggcg gcgatcttat cagatacctt
     5821 cgctccaccg agaatggcaa tgaagggccg cttgggattg gccgtcgctt tccctaaata
     5881 gtgcagttcc ttctgcatca ggtagcccgc cgctctttgc tggaaaagtc gcgcgactcc
     5941 ttcagtagaa gcatgcgccc ggtgagccga gccgaaggcg tcgttgacgt agaggtctcc
     6001 taatttcgcc aactgggccg agaaagcggg atcatttttt tcttcttccg agtgaaaccg
     6061 cagattttct aagagcaaac attcgctgtc ccgaagattt gcgactgctt gttctgcgac
     6121 cggcccgaca cagtccgtcg caaaggcaac ttttttgcct aagagttctt ccagtcttcg
     6181 cgccaccggc aaaaggctga attctggcgt cggcttccct ttgggacggc ccagatgaga
     6241 gcataaaatc gcccggccac cgtcggcgag aatttttttg atggtaggta gcgaggcgac
     6301 gatacgtatg tcgtcggcaa cgcgcccgtt ttccagcggc acgttgaaat cgacccgcac
     6361 caaaacgcgc ttgccgcgta acttcaggtc gtcaatcgag agcttggcca tgttatcagg
     6421 ttggttcacc ccgctattaa atatggtcta ttcccttaag ttactacgtc ggtgtcgact
     6481 tcctgccctc tcaatcgaaa cacacgtaag tagttgcgca atgactgttg cactcaaggt
     6541 aatcgccgga tgaatgtctt tatccgtcgc cgacgcggcc cagatgccca ctccccccac
     6601 gaccagtgaa ttcaggaaga caatcgggaa aaacccctgc cggttattgc cgagatgcac
     6661 gcccacgggc ataaaagtcg cttctccgat gggagctccg ataatagcac cttcaaatct
     6721 gctaaactct ccgtcatccg atccaccgag tattccggct ccaatacccg ccccggcgaa
     6781 gaagccgatc gccgcaaaga aaaggccgat accgattggc tgggctatgg agggctgtgg
     6841 ccgttcgccg tgctcaccat ccgaattgga tacaaaaaaa tgctggtcgc gtttttgatt
     6901 accaaaattc ggataagcga gtctcgccgc ttcgagtcgt gcaattgaaa atcgcagcgg
     6961 gcctattgtt tgaaccattg gaactgacac aaccgggtgc cgaccgctga gctgattgaa
     7021 gcgcggtttc tccctatttt cctctgctgc ggctgggatg gcgtgcaaca acagagcaag
     7081 ggggatactg aaatcaagaa taaagtgcgt ttttccaaat aagaaccgca ggggctgcat
     7141 gattcgacac ctttctcaat ggtgtcactc taacgggccg ccaccgtcgc aatccgctcg
     7201 gtcttatccc cgacggcacc agccatcatt atctgaattg tctcgaccat ccgcacagaa
     7261 aaggcccact cgttgtcgta ccaggcgaaa acttttacca ggtttttacc tatcaccatg
     7321 gtcgcctgcg cgtcaaagac tgcggagtag gggttgccca ccacatcggc ggagacgatc
     7381 gggtcctcgc aatactgcag gatgcctttc atcccgcctt cggcggcctc tttcattgcc
     7441 tggttcacgg aggcggcagt tacatctttt tcgagttcgc aggtcaagtc gaggagcgag
     7501 ccgtcggaaa tcggcacccg caccgcgacg ccatccaact tcccctctaa ttcaggaata
     7561 acctgcccga tcgccttggc cgcgccggtg gtggtcggga tgatggaata agccgccgcc
     7621 cgcgcccgcc ttaagtcttt gtgcggctgg tcgaggatcc gctggtcatt ggtgtaagcg
     7681 tg
//
LOCUS       DBNGZD010000042         2342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_284473, whole genome shotgun sequence.
ACCESSION   DBNGZD010000042 DBNGZD010000000
VERSION     DBNGZD010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_284473"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16GM_01240"
     CDS             <1..513
                     /locus_tag="AC16GM_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR2922330.1"
                     /translation="INEYAGQTIHVRPMKAFPLIKDLVTDVSWNYEVAREIVPFTPPP
                     GERAPFTIYEKDVERVHEFRKCIECFLCQNVCHVLRDHGAQSQYYGPRFMVKIAALEM
                     HPKDTLKRTDLLRGQGGIGYCNITKCCEELCPEHIHITDNAIIPLKERVADDYFDPLR
                     WLVKKLRGKQ"
     gene            complement(541..2295)
                     /locus_tag="AC16GM_01245"
     CDS             complement(541..2295)
                     /locus_tag="AC16GM_01245"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HIR2922331.1"
                     /translation="MPGICGVFADWPVDRLDKILTGLLTPLASGDHCRSDRFVSPGAA
                     LGRVHLGVFNTAPQPLFSFDRKQALLFYGEIFSGLPAAARDDQSTPQQVLEQLQRGRE
                     PDMAAWNGSFVFASYDFTRKRLLLGNDRYGLRPLYFATFPGGFAFSGSVRSLLSIPAV
                     PRTLNEEALGEFLTLGYLLGNHTLFSGVHLLPPAALLTWEKGPLRVQTYWQADFTQSS
                     RRWRRGEACSQAALLLQQTVERQTRSPKQIGLFLSGGLDSRVLAATARRCGRQLSTFT
                     FDYWNSPERELAARVAQAVQMSNEFLPLTRESYWSQVEAGVRATDGQVNCRNFMGIET
                     NPAVSRKTPVVFSGTGGNAVFGYLSFGILSLLFPWSHETFAARFYRRLATGLNRSELV
                     KLWGPRRGNVLWERGWEGFRRVCAAYRDQPPLVKISRFFFDQMHRRMVFLGLVADHSF
                     LEYRLPLYDYDLVDFMLSMPERERLLARFYRRLLTTHFPEVAVIPYSRTGLPVPAGTS
                     AIFWHKGLQLLLRRAKALPIPLDQELPEEWQKRMEEKFNFLEKTGFQKPVLNQNFSSF
                     SGRKKGLLLTLGIFLDEL"
BASE COUNT          575 a          675 c          662 g          430 t
ORIGIN      
        1 atcaatgagt atgccggcca aacgatccat gtccgtccga tgaaagcgtt cccactcata
       61 aaagacttag tgaccgacgt ttcctggaac tacgaagtag ccagagagat tgtgcctttc
      121 accccgccgc cgggagagcg ggcgccgttt acgatttatg aaaaggacgt ggagcgggtg
      181 cacgagttcc gcaagtgcat cgagtgtttt ctctgccaga acgtctgcca cgtcctgcgc
      241 gaccacgggg cacagtccca gtactacggc ccgcgcttta tggtgaaaat cgcggcactg
      301 gaaatgcacc cgaaagacac gctcaagcgg actgaccttt tgcggggcca aggcggcatc
      361 ggttactgca acatcaccaa gtgctgcgag gagctctgcc cggagcacat ccacatcacc
      421 gacaacgcta tcatcccctt gaaggagcgc gtcgccgacg actacttcga cccgctgcgg
      481 tggctggtga agaagctacg cgggaagcag tagctgaacc ccgcagagaa ggttaccccc
      541 ttagagctca tctaaaaaga tacccagagt cagcaggagt cctttttttc ttccggagaa
      601 cgaagaaaaa ttttgattca aaacgggttt ttgaaagcct gttttttcca aaaaattgaa
      661 cttctcctcc atccgcttct gccattcttc cggtagctct tggtccagag gaattgggag
      721 agctttggct ctccgcagca gaagctgtag acccttgtgc cagaagatgg ccgatgtccc
      781 cgcaggcaca ggcaacccgg tacgggagta gggaattaca gccacctccg ggaaatgcgt
      841 ggtcagcaac cgccggtaga aacgggccag cagtcgctcc cgctccggca ttgaaagcat
      901 gaagtcgacc agatcgtagt catacagggg aaggcgatat tccaagaagg aatggtcggc
      961 gaccagaccc aggaacacca tccgtcggtg catctggtcg aaaaagaacc gggagatttt
     1021 caccagcggc ggttggtcac gatacgcggc gcatacacgt cgaaaacctt cccaaccccg
     1081 ctcccagagc acattacccc gacggggtcc ccacaacttc accagctccg accgattcag
     1141 tcccgtggcc aaacgccggt agaatcgggc agcgaatgtt tcatgactcc agggaaagag
     1201 gagtgagaga attccgaagg aaaggtatcc gaacacagcg tttccgcccg ttccggagaa
     1261 caccaccggt gtttttcggc tgaccgctgg gttggtttcg atgcccatga agttccggca
     1321 gttcacctgc ccgtcagtcg cccttacgcc ggcttcgacc tgagaccagt acgactcacg
     1381 ggtgagtggc aagaattcat ttgacatctg aaccgcttgt gcgacgcgag ctgccaactc
     1441 ccgttccggc gagttccaat agtcgaacgt aaaggtggac aactgcctcc cgcagcgccg
     1501 ggcagtcgca gccagcacac gcgaatcaag accgccgctg aggaacaagc cgatctgttt
     1561 gggagagcgg gtttgtcgtt caaccgtttg ctggagaagc agagccgcct gggaacacgc
     1621 ttctcctctg cgccaccgcc gagacgactg ggtaaaatcg gcctgccagt aggtctgcac
     1681 ccgaagggga cccttttccc acgtcaggag tgccgccggc ggtaacaggt ggaccccgga
     1741 aaacaacgtg tggttgccga ggaggtatcc cagagtcaga aattctccca gagcctcttc
     1801 attgagcgta cgggggacag caggaattga cagaagcgag cggaccgaac cagaaaaagc
     1861 gaacccgccc gggaaagtag caaagtagag gggtcgcaac ccatagcggt cattccccaa
     1921 aagcaaccgc ttccgggtaa agtcgtagct ggcgaagaca aaggagccat tccacgcagc
     1981 catgtctggc tctctcccgc gctgcaactg ttccagaact tgctgcggcg ttgattgatc
     2041 atcccgggct gccgctggta gtcctgagaa aatttcaccg taaaagagca atgcctgttt
     2101 acggtcaaag gaaaacagcg gttggggtgc cgtattgaag acgccgaggt gcacccgccc
     2161 cagcgccgcg ccaggcgaaa cgaaccggtc gcttcggcag tggtcgcccg atgccaaagg
     2221 agtgagcaag ccggtaagga ttttgtcgag ccgatcaaca ggccaatcgg cgaaaacacc
     2281 gcaaatgccg ggcatggtaa aagttactta acgcgctccg taaaaagtcc gggccagagc
     2341 ag
//
LOCUS       DBNGZD010000043         4012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1534749, whole genome shotgun sequence.
ACCESSION   DBNGZD010000043 DBNGZD010000000
VERSION     DBNGZD010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1534749"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="rpsC"
                     /locus_tag="AC16GM_01250"
     CDS             complement(<1..785)
                     /gene="rpsC"
                     /locus_tag="AC16GM_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR2922332.1"
                     /translation="MGQKANPIGLRLGVIRTWNSKWFSTRTFASLLKEDLTIRRYTHR
                     RLENAGIANIEIIRAPKKISVDIHTSRPGIVIGRKGAEVDKLKEELQLLTKKDVSINI
                     IEVKQPELSARLVGESIAHQLEGRVSYRRAMKKAIAASMKMGAQGIKILCGGRLAGAE
                     IARSEKYLEGRVPLHTLRADIDYATATAHTTYGTIGVKVWIFRGEVLGKGRFTAEARQ
                     AGRLAEERPEPRPRRGRSERRPGRPDHRRGREGKTQTASPVAGG"
     gene            complement(797..1234)
                     /gene="rplV"
                     /locus_tag="AC16GM_01255"
     CDS             complement(797..1234)
                     /gene="rplV"
                     /locus_tag="AC16GM_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR2922333.1"
                     /translation="MEAIARARFQRMSPRKVRRVAALVRGKPVDEAIALLDFVPKAAA
                     VPLQKTIKSAVANALAVEGTAKLKSEDLAIRVVKVDGGPVLKRIRAVGMGRAYRIRKP
                     TCHITVVVAGEPKPVQLRGARRTTALKTPKPKGPDKEAPAARR"
     gene            complement(1240..1578)
                     /gene="rpsS"
                     /locus_tag="AC16GM_01260"
     CDS             complement(1240..1578)
                     /gene="rpsS"
                     /locus_tag="AC16GM_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR2922334.1"
                     /translation="MARSLKKGPYVDAKLMKKILKLNETGVKTVLKTWARRSTIAPEL
                     VGHTLAVHNGNKFIPVYITENMVGHKLGEFSPTRTFRGHSGKLAAKEAEGGAAPPPPA
                     PAPGEKAPKK"
     gene            complement(1583..2404)
                     /gene="rplB"
                     /locus_tag="AC16GM_01265"
     CDS             complement(1583..2404)
                     /gene="rplB"
                     /locus_tag="AC16GM_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR2922335.1"
                     /translation="MGIKTYKPFTPSRRWITVSDFAEITRSQPERSLLEPLRKSGGRN
                     NDGHITARYIGGGHRQFYRVLDFKRDKANVPAKVAAIEYDPNRSARIALLHYADGEKR
                     YIIAPLDLAVGDTLVAGPEAEIRPGNAVPLARVPLGTMVHNVELRRGAGGQIARSAGA
                     SVQLVAKEGNYGHLRLPSGEIRLVRLENYATIGQVGNLDHEKISLGKAGRTRWLGYRP
                     RVRGVAKNPVDHPMGGGEGKSSGGRHPCSPWGLKSKGQKTRKHKPSDRYILKRRD"
     gene            complement(2404..2700)
                     /gene="rplW"
                     /locus_tag="AC16GM_01270"
     CDS             complement(2404..2700)
                     /gene="rplW"
                     /locus_tag="AC16GM_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR2922336.1"
                     /translation="MKDARTVILRHLLTEKGTRLREKEHKYFFHVATGANKLEVKRAV
                     ETLFKVNVTGVNTQIVRGKTRRYGFRQRTGTRPNWKKAIVQLKTGQTIEALEKI"
     gene            complement(2697..3326)
                     /gene="rplD"
                     /locus_tag="AC16GM_01275"
     CDS             complement(2697..3326)
                     /gene="rplD"
                     /locus_tag="AC16GM_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR2922337.1"
                     /translation="MIVAKVYNSAGEEKEKTALSAALFGVTPHRPVLYQYVKMYLGNQ
                     RQGTHATKTRAEVSGGGRKPWRQKGTGRARSGSNTSPVWVRGGSAHGPKPRDYETKMP
                     QQARRLALKSALSAKAAAGKIVLFEPPSLEKPKTKILVAYFSKIGVLPGRTLVLAEGK
                     DERLGLSCRNIPGVTFKRAAAVNAYDLWIADSVLLTKPALAAVEEVFSG"
     gene            complement(3362..3994)
                     /gene="rplC"
                     /locus_tag="AC16GM_01280"
     CDS             complement(3362..3994)
                     /gene="rplC"
                     /locus_tag="AC16GM_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR2922338.1"
                     /translation="MPGAILGRKIGMTQIFNAAGDAVPVTVVEAGPCPVVQVKTAAKD
                     GYGAVQLGFGARRKNLFTRPLAGHFARHKVEPTHLLREVSLRPDEKIEPGQAVTVELF
                     QVGETVNVIGVSRGHGFQGTVKRHHFKGGPKTHGQSDRWRAPGSIGASSFPSRVLKNV
                     RMAGRMGGNRVTVRNLSVVGVDAERHLLLISGSVPGKPRGWLLVSKVPKR"
BASE COUNT          645 a         1289 c         1192 g          886 t
ORIGIN      
        1 ccgccagcga cggggcttgc ggtttgggtt ttgccctcgc ggccacggcg gtggtccggc
       61 cgccccggac gcctttcgct ccggccccga cggggacggg gttcgggccg ttcctccgcc
      121 agccgccccg cctgccgggc ttcggcggta aatcggccct tccccaacac ctcaccccgg
      181 aaaatccaga ccttgacgcc gatggtaccg taggtggtgt gggcggttgc cgtggcgtag
      241 tcgatgtcgg cccgcagcgt atgcaaaggt acgcgccctt ccaagtactt ttcggaacgg
      301 gcaatttccg caccggccag ccggccgccg cagaggatct tgatcccctg cgcgcccatt
      361 ttcatggagg cggcgatcgc cttcttcatg gcgcggcggt aggagacccg gccttccagc
      421 tggtgcgcga tgctctcccc taccagccgg gccgacagtt ccggctgctt gacttcaata
      481 atattgatgg agacatcctt tttggtcaaa agctggagtt cctctttcaa tttatcaacc
      541 tcggcacctt tacggccgat gacaattccg ggacgggaag tgtggatgtc taccgaaatt
      601 ttcttcgggg cgcggatgat ctcgatattg gcgatgccgg cattttccag ccggcggtgg
      661 gtataccgcc ggatggtcaa atcttctttg agcagagacg caaaggtgcg cgtggagaac
      721 catttggagt tccaggtccg gatcactcct aaacgcagtc cgatgggatt ggctttctga
      781 cccactgctt tcgctcctac cttctggcgg ccggggcttc cttgtccggc cctttcggtt
      841 tcggcgtttt caatgcggtg gtccgcctgg ctcctcgcag ctggaccggc ttcggttcgc
      901 cagccacgac cacggtgata tgacaggtcg gcttgcgaat ccgataggcg cgccccatgc
      961 ccaccgcccg gatccgcttg agcaccgggc cgccatcgac tttgaccacc cggatcgcta
     1021 agtcttcgga tttcaacttg gcggttccct caacggctaa ggcattcgca acggctgact
     1081 tgatggtttt ttggagtggc acggccgcgg ccttggggac gaagtccaga agggcgatgg
     1141 cttcatccac cggcttgccg cgaactaacg ccgccactcg ccgcactttg cgcggggaca
     1201 ttcgctggaa tctggctcgg gcgattgctt ccattggact cacttcttgg gcgctttctc
     1261 acccggcgcg ggtgccggcg gcgggggtgc agcaccgccc tcggcctcct tggcggccag
     1321 ttttccggaa tggccgcgga aggtccgggt gggtgaaaac tcgcctaatt tgtgccccac
     1381 catgttctcg gtgatgtaga ccggaatgaa cttgttgccg ttgtgcaccg ccagcgtatg
     1441 acccacgagt tcgggcgcaa tggtggaacg ccgcgcccag gttttgagca ccgttttgac
     1501 cccggtctcg ttcaacttca aaatcttctt catcaacttg gcgtccacat agggaccctt
     1561 cttgagtgag cgtgccatag tattagtcgc gccttttgag aatgtaacgg tccgagggct
     1621 tgtgcttgcg cgtcttctgg cctttggatt ttaagcccca gggcgagcag gggtgccggc
     1681 cgccggacga tttcccttcg ccgccgccca tcggatgatc caccgggttt ttggccactc
     1741 cgcgcacccg ggggcggtaa cccagccagc gggtgcggcc cgccttgcct aaggaaatct
     1801 tctcgtgatc gagattgccg acctggccga tagtggcgta attctccagc cggaccagcc
     1861 ggatttcacc cgaaggcaag cgcaggtgac cgtaatttcc ctccttggcc acgagctgga
     1921 ccgatgcacc ggcgctgcgg gcaatctggc ccccggcgcc gcgccggagt tccacgttgt
     1981 gtaccatcgt ccccagaggg acgcgggcta aaggcacggc gttccccggc cggatctccg
     2041 cttcgggccc ggccacgagg gtatcaccga cggctaaatc cagcggggct atgatgtaac
     2101 gcttttcgcc gtcggcgtag tgtaaaagcg caatccgtgc tgagcggttc ggatcgtact
     2161 caatggcggc cactttcgcc ggaacgtttg ctttgtcgcg cttgaagtcg agcacccggt
     2221 agaactgcct gtgcccgccg ccgatgtagc gggcggtgat atgcccgtcg ttgtttcgcc
     2281 cgcccgattt gcgcaaaggc tccagaaggg aacgctccgg ctggctgcgg gtaatctcgg
     2341 caaaatcgga gaccgtgatc cagcgccgcg agggggtgaa cggtttatag gtcttgatgc
     2401 ccatcagatc ttctccaggg cctcaatcgt ctgtccggtt ttcagctgca ctatcgcttt
     2461 cttccagttc ggccgcgtgc cggtgcgctg ccggaagccg tagcgccggg ttttgccgcg
     2521 cacaatctgg gtgttgaccc cggtgacgtt tactttgaaa agggtttcca ccgcgcgctt
     2581 gacctccagt ttgttcgctc ccgtggccac gtggaaaaag tacttatgct ctttttcccg
     2641 gaggcgcgtc cccttctcgg tcaacaggtg ccgcaggatc accgtgcgcg cgtccttcat
     2701 ccggaaaaaa cctcctcaac ggccgccagg gccggtttgg tcaaaaggac ggagtcggcg
     2761 atccacaggt cgtaggcgtt gacggcggcg gcccgcttga acgtgacacc gggaatgttc
     2821 cggcacgaaa gtccgagcct ttcgtctttc ccttccgcca gcaccagggt ccgcccggga
     2881 agcaccccaa tctttgagaa gtaggcaacc agtattttcg ttttcggttt ttccaaactg
     2941 ggtggttcaa agaggactat tttcccggcc gcagcttttg cggaaagagc cgacttgagg
     3001 gccagccgcc gggcctgctg cggcattttg gtttcgtagt cgcgcggctt ggggccgtgg
     3061 gcgctcccgc cgcgcaccca gaccggtgag gtgttactgc cggagcgcgc gcgcccggtc
     3121 cccttctggc gccacggttt gcgccccccg cccgaaactt cggcgcgggt cttggtggcg
     3181 tgcgtcccct gcctttggtt ccccaggtac attttgacgt actggtacaa caccggccgg
     3241 tgcggcgtga ccccaaagag cgccgcgctc aacgccgtct tttctttttc ctctcccgca
     3301 ctattgtaga ctttggctac gatcatcatt tcagtttctc cgggaattcc cgactccggt
     3361 cttagcgctt cggcaccttg ctgaccaaga gccagccccg gggcttcccc ggcaccgatc
     3421 cggaaatcaa aaggaggtgc ctttccgcat ctacgccgac tacggaaaga ttccgtacgg
     3481 tcacccggtt gccgcccatc cgtcccgcca tccgcacgtt tttcaacacc cgcgagggga
     3541 aggaactggc cccgatcgat cccggcgccc gccagcggtc tgactggccg tgcgttttgg
     3601 gtccgccttt gaagtggtgc cgcttgaccg ttccctgaaa gccgtgaccg cgtgaaacgc
     3661 caatgacgtt caccgtctct ccgacttgaa agagctcgac ggtcaccgcc tggccgggtt
     3721 cgattttttc atcgggccgc agggacactt cccgcaagag atgagtcggt tccactttat
     3781 gccgcgcgaa atgacccgcc aaaggcctgg tgaaaaggtt tttccgccgt gcgccgaacc
     3841 ccagctgcac cgccccgtac ccgtccttgg ccgcggtttt gacctgcacg accggacagg
     3901 ggccggcctc gaccaccgtc accggcaccg catcaccggc ggcgttgaag atttgtgtca
     3961 ttccaatttt tctgcctaaa atcgcacccg gcattttcgc tctcagtctc cg
//
LOCUS       DBNGZD010000044         1684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1882012, whole genome shotgun sequence.
ACCESSION   DBNGZD010000044 DBNGZD010000000
VERSION     DBNGZD010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1882012"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="AC16GM_01285"
     CDS             complement(<1..358)
                     /locus_tag="AC16GM_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS N-terminal domain-containing protein"
                     /protein_id="HIR2922339.1"
                     /translation="MQQYWKIKSQYPEEIVFFRMGDFYEMFGEDAKSASQILGIALTS
                     RAHGDMEKIPLAGVPYHAADRYLAKLIAAGKKVVVCEQMEDPKLARGLVKRDVVEIIT
                     PGTVLLANAGAGERSTY"
     gene            complement(683..>1684)
                     /locus_tag="AC16GM_01290"
     CDS             complement(683..>1684)
                     /locus_tag="AC16GM_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR2922340.1"
                     /translation="KRIFPFLLILASPVVVGRELATAQIQQAEKFIAEHEYGKAYTVL
                     SELVAKDPKNAQAVLWAAEAAPTGVDAQQVLEQYQATGSLNLAVVSELVRLYYAIGDY
                     GKIMETTERVEKQLKNKPLPADVLFLAGQAAGALGSAEWAQYYFDLLQQQRPGSLFAG
                     WAELGSADLLAESQRTADAVSEYQTLSQRETEQATPVAIEQLTVLLVNTGDLDGAFTA
                     YNLYRDRYPDGLGLPRLQEKLRAVHDTGAVEELPDGGAQKRWSLQAGVFGNLENAQRM
                     LKRYQAAGYPVEMTSQVISGKKLFSIFIGSYATEAEARRKKAEIETKEKEVLKLVIR"
BASE COUNT          329 a          511 c          477 g          367 t
ORIGIN      
        1 agtaagtgct ccgctctccc gcaccggcat tcgcgagcag aaccgttccc ggcgtgatga
       61 tttccaccac atctcgcttg accagccccc tggctaattt cggatcttcc atctgctcgc
      121 agacgacgac ttttttcccg gcggcaatca actttgccag gtagcggtca gccgcatggt
      181 acggtacgcc cgctaaggga attttttcca tatcgccgtg ggcgcgggag gtgagggcaa
      241 tgcctaaaat ctgagaggcg gatttagcat cctccccgaa catttcgtag aaatcgccca
      301 tccggaaaaa gacaatctcc tccgggtact ggcttttgat cttccagtac tgctgcatca
      361 aaggggtgtc cgccttaagt ggattgtccg ctcttcgact tggctcagag tctttgaccc
      421 gggtcatcaa gcggtaaaag taagattttt tggggaaaag tcgaggggtg ctcccaatcg
      481 agcagttcta caaagggcgg ccgggggcgg tcgccctacg cgggcgagct cagacgcgcg
      541 tgggcgcggt cgatacgact tggctgaacg acccctcccc aaccgggccg tatggctgcc
      601 ctcccctcgg aggggagggt aaggaggatc ccaaaaacgc gtaggggacc tgtacgggtc
      661 tcccttcaat gggaagagcg aattagcgga tgaccaactt cagaacttct ttttccttcg
      721 tttcgatttc ggctttcttc ctgcgtgctt cggcctcggt ggcgtaggag ccgatgaaaa
      781 tcgagaaaag ttttttgccg gaaatcacct ggctcgtcat ttccacggga taccctgcgg
      841 cctgatagcg cttcagcatg cgctgcgcgt tttcaaggtt cccgaagact ccggcctgga
      901 gcgaccagcg cttctgcgcg ccgccgtcag ggagttcttc aaccgcaccg gtgtcgtgca
      961 cggcccgcag cttctcctgc aaacgcggca gccccaggcc gtcgggatag cggtcgcgat
     1021 agagattgta ggcagtgaag gcgccgtcaa gatcccccgt attgaccaac agcacggtca
     1081 attgctcaat ggccaccggc gtggcctgct cggtttctcg ctgcgagagc gtttgatatt
     1141 cactgaccgc atccgccgtc cgctgacttt cggccagaag gtcggccgaa ccgagctccg
     1201 cccagccggc aaaaagcgac ccggggcgct gctgctgcaa aaggtcgaag tagtactgcg
     1261 cccattcggc tgagcccagc gctccggcgg cctggccagc caaaaagagc acgtcggccg
     1321 gcagcggctt gttcttcagc tgcttctcca cccgctcggt cgtctccatg attttcccat
     1381 aatcgccgat cgcgtagtac aaccgcacca gttccgacac cacggcaaga tttagactgc
     1441 cggtggcctg atattgctcc agcacttgct gcgcatccac gccggtgggg gcggcctccg
     1501 ccgcccagag gacggcttgc gcgtttttgg ggtctttcgc caccagttcg gaaagaactg
     1561 tataggcctt tccatactcg tgctcggcaa tgaacttctc ggcttgctga atttgggctg
     1621 tcgctaactc acgaccaaca accacagggc tcgccaatat caggaggaat ggaaaaattc
     1681 tttt
//
LOCUS       DBNGZD010000045         3808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_562584, whole genome shotgun sequence.
ACCESSION   DBNGZD010000045 DBNGZD010000000
VERSION     DBNGZD010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_562584"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="AC16GM_01295"
     CDS             complement(<1..832)
                     /locus_tag="AC16GM_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922341.1"
                     /translation="MSTGRAQIGPPVITVAYPKDSARLGASDSTFIFGQVIPRCSLFI
                     NGQSVPVYRNGTFVAFLPLTPGPFTFNLAASKGPDTTFKQVLVFVPEPVTTPSPETLR
                     IAGGLRPSDDLELRAGDVLTVAFRGTPGCQAFFRIDGLTDALPMAEAGAVVESSATES
                     VFQAHLADTVRPVRGFYRGAYQIQPADSIDTARIIVTLVRSPVTADAAEFAALIRDTT
                     LIRIEAVAHGTPDTLCFSFCVRDTAPGRISIRRSAVPQVVELTDSIQILRTGPGQGFL
                     A"
     gene            complement(873..1877)
                     /locus_tag="AC16GM_01300"
     CDS             complement(873..1877)
                     /locus_tag="AC16GM_01300"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HIR2922342.1"
                     /translation="MKIQAEILELRLKHTFRIASGATDLKRNVIVHIGEGIGEAAPAL
                     YLGEWYKSVLACLEFLEPELGDDPALIEEIEDRISQKIGFNFAPKAAIDLALHDHLSK
                     KAGVPLFQFLGLTKSEEKKTSVTIAIAEPKEMAQRAVEAKTFPILKVKVGVPGDMEKV
                     TAVRRARPDAAIRVDANCGWTLVEALEKMPLLEQLSVELVEAPLDSVDIEGLRLLRTK
                     SPLPIFLDEGVMTAKDIPGYAPVADGINVKLMKCGGLREALRMIREARRLNLKVMLGC
                     MLESAIGVTAAAHLAGLADYLDLDSILLTDNDPYQGAAIENGVLRLPQRPGIGVVKVG
                     "
     gene            complement(1874..2839)
                     /locus_tag="AC16GM_01305"
     CDS             complement(1874..2839)
                     /locus_tag="AC16GM_01305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HIR2922343.1"
                     /translation="MPRTPGKKLLRPRALEIGGTIGIVAPGSPPGRRRLRAGVAFLKQ
                     QGFKVKIHSQCYRSFGFLAGRDEVRAQALHAMFADPDIDAILCARGGVGCLRLLPLLD
                     FGLIRRNPKIFVGYSDLTALHLAIYQRCRLVTLHGPMVASELGPKVPHFTARHLFRSL
                     RDGRPPGVVSIGGKRKPFTIIRPGCASGPLLGGNLSLVCRLVGSGYLPDFAGALLFLE
                     DINEEPYRIDNFLSQLRLSGILNHVAGVVFGNFVDCEPEDAEKPSFSLREVLFDHFSH
                     MPYPVIWGVPFGHGKKTITIPHGVQGTVDTRARRFSIDEAAVTSA"
     gene            complement(2844..>3808)
                     /gene="alr"
                     /locus_tag="AC16GM_01310"
     CDS             complement(2844..>3808)
                     /gene="alr"
                     /locus_tag="AC16GM_01310"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR2922344.1"
                     /translation="TLKAGAKLLGVALLEEGVALREAGVRAPIVVLYPEAAERAPYYV
                     PYGLMPTICDASFGQHYAQALKKRGRRSEFYIKVETGMHRYGLPVNQNLALLQRLKNF
                     PALRLAGAFSHFADSGEDGDYSQVQLARFRSALSAFAQAGFPPPLASIANSGALLEVS
                     HSALDMARIGILLFGVPPVPHHKVNLPVKPILSIKSRIAFLKEVPSGTPISYGRTFTT
                     ERPTLVATLPLGFADGFPRQLSNRGAVLVRGKKAPVIGRVCMDAFMVDVTGIVGVKVG
                     DEVVLVGWQGKEAIDLWALASAIDTTAYEVVSRLGKRLPVDYRL"
BASE COUNT          834 a         1146 c         1120 g          708 t
ORIGIN      
        1 tggccagaaa cccctgaccc gggccggtcc gcagaatctg gatgctgtca gtcaattcaa
       61 ccacctgcgg cactgcgctc ctgcggatgc tgattcttcc cggagccgtg tcccgaacac
      121 aaaaagagaa gcagagcgtg tccggagtgc catgcgcaac cgcttcaatc cggatcaacg
      181 tagtatcccg gattaaagcc gcaaactccg cggcatcagc cgtcacggga ctgcgcacga
      241 gcgtcacgat aatgcgggcg gtatcgattg aatcggctgg ttgaatctga tacgcgccgc
      301 gatagaaacc gcgcaccggc cgaacggtgt cggctagatg ggcttgaaaa acactttcgg
      361 tcgccgatga ttcgaccacc gctccggcct cggccatagg aagtgcgtcg gtgaggccgt
      421 cgatgcggaa gaaagcctga catccgggcg tcccgcggaa agcaaccgtg agaacgtcgc
      481 cggcgcgaag ttctaaatcg tcgctgggac gcaagccgcc ggcgatgcgc aacgtctccg
      541 gcgacggtgt cgtcaccggc tccggcacga aaaccagaac ctgtttgaaa gtggtgtcag
      601 gccctttgct cgcggcgaga ttgaaagtga acggcccggg ggtcagaggc aagaacgcga
      661 cgaatgttcc gttgcggtaa accggaacgg actgcccgtt gatgaaaagg gagcagcgcg
      721 ggataacctg tccgaaaatg aaggtggaat cggacgcacc cagccgcgcg ctgtctttgg
      781 ggtacgccac ggtgataacg ggcggcccga tctgagcgcg gccagtcgac acagcaagag
      841 agaaaattaa aaagagaaga aagaccgaac gcttagccaa ctttcaccac cccgatcccc
      901 ggtctttgcg gcaagcgcaa gacaccattc tcaatcgctg ctccttgata gggatcattg
      961 tcggtcaaaa ggatgctgtc taagtcgagg taatcggcca aaccggccag gtgcgccgca
     1021 gccgtaactc cgatagcact ttccagcatg caccccagca taactttgag gttcaaccgc
     1081 cgggcttcgc gaatcatccg cagcgcctcg cgcaggccgc cgcacttcat cagtttgacg
     1141 ttgatgccat ccgccaccgg cgcgtagccc ggaatgtctt tggccgtcat cactccttca
     1201 tccaggaaga tcggcagggg cgatttggtg cgcaagagcc gcagcccctc gatatcgaca
     1261 gaatccaaag gcgcttccac caattcaacg ctcaactgct ccaatagcgg catcttctct
     1321 aacgcttcca ccaaggtcca gccgcagttg gcatcgaccc ggatcgcggc atcgggccgc
     1381 gcccggcgga ctgcggtgac cttttccata tctccgggaa ctccgacctt aaccttgagt
     1441 atcggaaaag tcttcgcttc cactgcccgt tgagccatct cttttggctc ggcaatggct
     1501 atggtcaccg aggtcttttt ttcttcggat ttcgtcaatc ccaaaaactg aaagagcggc
     1561 acgcccgctt ttttgctcag gtggtcgtgg agggcgaggt caatggccgc tttcggcgcg
     1621 aagttgaaac cgattttctg actgatgcga tcttcaattt cctcgatcaa tgcggggtca
     1681 tcacccagct ccggctctaa aaattccagg caagccaaga ccgatttata ccactcaccg
     1741 agatacagtg cgggcgctgc ttcgccgatg ccctcgccga tatggacgat gacgttgcgc
     1801 ttgagatcgg tcgccccgct ggcaatgcgg aaagtgtgtt tgagtcttaa ctccagaatt
     1861 tcggcctgaa tcttcatgcg ctggtgaccg ccgcctcgtc gatggaaaag cgtctcgctc
     1921 tcgtgtcgac cgtcccctgc acgccgtggg gaatggtgat cgtttttttg ccgtggccga
     1981 agggcacacc ccagatgacc gggtagggca tgtgcgagaa gtggtcaaac aacacttcgc
     2041 gcaaagagaa agacggtttc tctgcgtctt ccggctcgca atcgacgaaa ttaccaaaca
     2101 ccactcccgc tacgtggttc aagatccctg acagtctcaa ctggcttagg aaattatcaa
     2161 tgcggtaggg ctcttcgttg atgtcttcca gaaagaggag cgcaccggca aagtcgggca
     2221 ggtagcccga gccgacaagc cggcagacta aagagaggtt cccgcccaaa agcgggccgg
     2281 aggcgcagcc ggggcggatt atcgtgaacg gttttcgctt gccgccgatg gacacaaccc
     2341 ctggcggcct tccatccctg agcgagcgaa aaagatggcg cgcggtgaag tgcggcacct
     2401 ttggtcccaa ctccgaggcc accatcggcc cgtgcagggt taccagtcgg cagcgctggt
     2461 agatggctag gtgcaaggcg gtcagatcag agtacccgac aaaaattttg ggatttctcc
     2521 gtatgagacc gaagtcgagc agcgggagca gccgcagaca gccgacgccg ccgcgcgcgc
     2581 agagaatcgc atcgatatcc gggtcggcaa acatcgcgtg cagggcctgc gccctgacct
     2641 catcgcgacc ggccagaaag ccaaaactgc ggtaacactg cgagtggatt ttgactttga
     2701 agccctgctg cttaaggaaa gcgactcccg ctctcaggcg cctgcggccg ggcgggctgc
     2761 cgggcgccac aatcccaatg gtccccccga tttcaagcgc gcgcgggcga agcaacttct
     2821 tccctggagt tcggggcatc agatcacaag cggtaatcca ccggcaaacg cttgcccagg
     2881 cggcttacca cttcgtaggc ggtggtgtca atggcggaag ccagcgccca aagatcaatt
     2941 gcttcttttc cctgccaccc gaccaaaacc acttcatcgc cgacttttac tccgacaatg
     3001 ccagtgacat cgaccatgaa ggcgtccatg cagacccggc cgatcaccgg ggctttcttc
     3061 ccgcgcacca gaaccgcgcc gcggttggaa agctgacgcg ggaatccatc ggcgaaaccg
     3121 aggggaagcg tggcgacgag cgtcggccgt tcggtggtga acgtgcggcc gtagctgatg
     3181 ggagtccccg acggcacttc cttgaggaaa gcaatccggc ttttgatcga gagaatcggt
     3241 ttgaccggca ggttgacctt gtgatgcggc acgggcggca cgccgaagag caaaatgccg
     3301 atccgcgcca tatcgagagc cgaatgggag acttcgagca gcgccccgct attggcaatg
     3361 gaagccagag gcggcggaaa accggcttgg gcaaaggccg aaagcgcact gcggaagcga
     3421 gccagttgta cctggctgta gtctccgtcc tctcccgaat cggcaaagtg cgagaatgcc
     3481 ccggccaagc gcagggcggg aaaattcttc agccgctgca gaagcgccag attctgattg
     3541 accggtagcc cgtagcggtg cattccggtc tcgactttta tatagaactc gctgcggcga
     3601 ccacgcttct tcaatgcttg agcgtagtgc tgaccgaaag aggcatcaca gatggtgggc
     3661 atgaggccgt acggcacgta gtagggtgcg cgctccgccg cttcggggta gagaaccacg
     3721 atcggcgccc ggaccccggc ttcgcggagc gccacccctt cctctaaaag cgccacgccc
     3781 agaagcttcg ctccggcctt aagcgtca
//
LOCUS       DBNGZD010000046         6172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_770893, whole genome shotgun sequence.
ACCESSION   DBNGZD010000046 DBNGZD010000000
VERSION     DBNGZD010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_770893"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="der"
                     /locus_tag="AC16GM_01315"
     CDS             complement(<1..1152)
                     /gene="der"
                     /locus_tag="AC16GM_01315"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR2922345.1"
                     /translation="MPIPIVAIIGRPNVGKSTLFNRLVKRRVAVVHDLPGVTRDRNYF
                     LASWNRKQFYLVDTGGLVPQAQSDIERLVKEQAEIALSEADLVLFTIDGQVGAQTVDI
                     AIARKLKKSAKKVVVVANKVDAQRQEYDTYELTRLGLGEAVTVSALNGRNVAELLDSI
                     AQFLPETDGEAVDEETINVAVLGRPNVGKSSFVNALLGKPKLVVSEKPGTTRDAIDTL
                     FVRDGRSYTFIDTAGLQRKARIKQDLDYYVSLRTLRSIERCDVALALIEGDLGLVRQD
                     IRIAEQVDERFKGLIWVVNKWDLVEKDTSTADEFTKAIHRFAPGFDYAPVIFVSAKTK
                     QRVTNVLSLVQTVHQEYHKRVATSDLNATISAAVKKQPPAAVMGKHVRIL"
     gene            complement(1240..2514)
                     /locus_tag="AC16GM_01320"
     CDS             complement(1240..2514)
                     /locus_tag="AC16GM_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR2922346.1"
                     /translation="MPDEQLRMFVFLSELLYRRVVDEKLRPVGKVADFKVGLRELFPK
                     IEALVLRQHGTKTPLALSWELVKSVNGHQIVVTEEAGQKLGPLATAADELLLRDEILD
                     KQVVDTAGAKLERANDLHLLINDGELRLVHVDVGLRGILRRLGWTRAFDAVTNWLFAY
                     QIPQKLISWKYIQPLAHDPEKKRLKLNVTLRKLSEIHPADLADIIEELDKHKASAVFQ
                     TLDVESQAEALEELDPKLQKTLIEQVDPSRASDIIEEMAPDEAADLLADISEEKKEVV
                     IRGLEAESRTELTELLTYPEGTAGALMTKDFFCARAGSTVAQAIELLKTLHAEVEMFY
                     YAYVVDENGRLVGALSLRNMLLHDPQAKLEEIMHRHLITVRLTNKQKTVFKKFQKYDY
                     LALPVVDDKGILKGIVTLKDALAAVHPEIVGK"
     gene            complement(2514..2690)
                     /locus_tag="AC16GM_01325"
     CDS             complement(2514..2690)
                     /locus_tag="AC16GM_01325"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaI-LiaF-like domain-containing protein"
                     /protein_id="HIR2922347.1"
                     /translation="MGRRGGEIWFPLILIVLGGIFLLKNLGVVSVSLHRWWPVIFILW
                     GASILLRRSTRNNT"
     gene            complement(3037..4074)
                     /locus_tag="AC16GM_01330"
     CDS             complement(3037..4074)
                     /locus_tag="AC16GM_01330"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR2922348.1"
                     /translation="MKPLYHYVGAIHIHTTYSDGSKPIAEVIAIGRNAGLDFMLFSDH
                     MTLKGLEEKKEGWYDRTLVLIGYEINDREDRNHLLAFNLRQVLPADLKPKEYVRKVRE
                     HGGLGIIAHPDEVRNKLPQYRPYPWNAWDVDGFDGIEIWNQMSEWMEKLTRWNRLAMV
                     FRPRRSLYGPTPRILARWDEYNQKRPTVGTGGIDVHAHPHKFGPFTIIIFPYEVQFKS
                     IRTHVLLDKPLSKEFPEAKRQLLEAIRHCRVFISNYRWGEAMNFRFWAENGGDEVTIG
                     ETISLKKSVHLHAHFPRPCESRLIHNGQLATTAQGMVLHYEAKLSGVYRIEAYRKGRG
                     WIYSNHIRVSG"
     gene            complement(4071..5447)
                     /locus_tag="AC16GM_01335"
     CDS             complement(4071..5447)
                     /locus_tag="AC16GM_01335"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2922349.1"
                     /translation="MSGRDRLTIFGWAAYDFANTIFSMNIISLYFSQWVIVDQGKEDI
                     WYSAAYSLSMVFVAVTMPILGAVSDHARKKLPFLFWWTLACCLLTLLIGWVSRRPGPG
                     NGTVLLALGLFVLANYFYEGGNVFYNSLLPFISTSTNIGKISGIGVALGYVGSIAGML
                     LVRPFVEGKVHLGHLFGQPERGRAASFFPTAVFFFLFALPIFFFVREKAGRAVRLSRL
                     YILAAFRKVKNGLVDSRKYPGVVRFLVADFLFEDAIATTIVYMAVYLEKVVGLPDVKK
                     TIFLSVATLCAVGGSFLCGLIVDRIGPKRTLRFVVFGWIVSLLLIAISRNLTVLWILA
                     GAIGIFLGSTWTSARPLLASLVPAKRVGQFFGLYALSGRAAAILGPLVWGFCVLYFKV
                     DSAIVQAMISGLERLGVTFSPQVLPSIQYRIAIVALAMLMAVGLILFRKVPDRFQKRV
                     KLSVEPHQ"
     gene            complement(5452..5892)
                     /locus_tag="AC16GM_01340"
     CDS             complement(5452..5892)
                     /locus_tag="AC16GM_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922350.1"
                     /translation="MGAACLVVLGLLGQVPRDVAPALQPSPSFYSLPNTFELTFSRFS
                     PSMQAADASPGQKKKTSDCWFGGDKVQHALVSTYLTFFIYQVNREIFDEKKSEARVVA
                     VTTTFFIGTAKETYDRTVRRTRFSLKDLVADGVGIAFGLWATTW"
     gene            complement(5892..>6172)
                     /locus_tag="AC16GM_01345"
     CDS             complement(5892..>6172)
                     /locus_tag="AC16GM_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922351.1"
                     /translation="FQVWNAERPIENVLTVQGELDGEFLSSRKDDLYPGNRLPAVLRL
                     DFKARFKIKDLTIYYFFENLTNRPYTLRGITPAVERGHSFGFAWEFFD"
BASE COUNT         1400 a         1801 c         1671 g         1300 t
ORIGIN      
        1 cagaatgcga acatgtttgc ccataaccgc tgccggtggc tgttttttga ccgcggcact
       61 gatggtcgca ttcaggtcgc tggtggccac gcgcttgtgg tattcctggt ggactgtttg
      121 cactaaggac agcacgttgg tgacccgctg tttggttttg gccgaaacga aaataaccgg
      181 tgcgtagtca aaaccgggcg cgaatcggtg aatggccttg gtaaattcat ccgccgtact
      241 ggtatctttt tccaccaggt cccacttgtt cacgacccag atcaatcctt tgaaccgctc
      301 atccacctgc tccgctatgc ggatgtcctg ccttactaac cccagatccc cctcgattaa
      361 cgccagcgcc acgtcgcacc gttcgataga ccgcagagtg cgcagggaga catagtagtc
      421 gagatcctgc ttgattctcg ccttccgctg caagccagct gtgtcgataa aggtgtagct
      481 ccggccgtcg cggacaaaaa gggtgtcgat ggcgtcccgc gtcgtgcccg gcttttcact
      541 gacgactaac ttcggtttgc ccaaaagggc attgacgaag gacgatttcc ccacgttcgg
      601 acgtcctaac accgcaacgt taattgtctc ctcgtcgacc gcctctccat ctgtttcggg
      661 cagaaactga gcaatggagt cgagcagctc cgccacgttg cgaccgttga gcgccgaaac
      721 cgtcaccgct tcgcccaaac cgagacgggt gagttcgtag gtatcatact cctgacgctg
      781 cgcgtcaact ttattggcca caaccacaac tttcttggcg ctctttttca gcttccgggc
      841 gatagctata tcaaccgtct gcgcacccac ctgtccgtcg atcgtaaaga gcacaaggtc
      901 ggcctcgctc aaggcaattt ccgcctgctc tttgaccagc cgttcgatgt cggactgcgc
      961 ctgcggcacc aagccgccgg tgtcgaccaa atagaactgc ttgcggttcc aggaagcgag
     1021 aaagtagttc cgatcgcggg tgacgccggg cagatcgtgc accaccgcca cccggcgctt
     1081 caccagccgg ttaaagagcg tcgatttgcc aacattgggc cgaccgatga tcgcaacgat
     1141 tgggataggc ataaccaagg accgcttcgg ggaaatatac tgcgctgcct tgagaaaatc
     1201 tccgaaaaaa cgaactccgg cacaagggag agcggaagac tacttcccga cgatctccgg
     1261 gtgcactgcc gccaaagcgt ctttgagggt gacaatccct ttcaggatgc ctttgtcatc
     1321 caccaccggc agggccaggt agtcgtactt ctggaacttc ttgaagacgg tcttctgctt
     1381 gttggtcaga cgaacggtga tcaaatgccg atgcatgatc tcttccagtt tggcttgcgg
     1441 atcgtggagc aacatgttgc gcagggaaag tgcacccacc aagcgtccgt tctcatccac
     1501 gacgtaggcg tagtagaaca tctccacttc ggcgtggagg gttttgagca actctatcgc
     1561 ttgcgccacc gttgacccgg cccgggcgca gaaaaaatct ttggtcatca aagctccggc
     1621 cgtgccttcg ggataggtca agagctcggt caactccgtc cgcgactcgg cttccaaccc
     1681 ccggatgacc acctcttttt tctcttcgga gatgtcggct aagaggtcgg ccgcttcgtc
     1741 gggcgccatt tcctcaatga tgtcggaggc gcgggacgga tcgacttgct cgatcaaggt
     1801 cttttgcagt tttggatcga gctcctccaa cgcttcggcc tggctttcca catccaacgt
     1861 ctgaaacacg gccgaggctt tatgcttgtc cagttcctca atgatgtcgg ccaagtcggc
     1921 cgggtggatt tctgaaagct tgcgcagggt gacgttcaac ttcagtcgct tcttctccgg
     1981 gtcgtgcgcc aagggctgaa tgtatttcca ggagatcaac ttctgcggaa tctggtaggc
     2041 aaagagccag ttggtgaccg catcgaacgc gcgcgtccag cccaggcgcc ggaggatgcc
     2101 ccggaggccc acgtcgacgt gcaccagccg cagttcgccg tcgttgatga gtaagtgcag
     2161 gtcgttcgcg cgttccagtt ttgctccggc cgtatcgacc acctgcttgt ccaaaatttc
     2221 atcgcgaagc aaaagttcgt ctgcagcagt ggcgagcggt cccagtttct ggccagcctc
     2281 ctcggtgacg acgatttgat gcccgttgac gcttttgacc agctcccacg agagtgccag
     2341 gggtgttttc gtaccgtgct gccggaggac gagcgcctca attttgggga agagttcccg
     2401 caaacccacc ttgaaatcgg ccaccttccc caccgggcgg agcttttcat ccaccacccg
     2461 ccggtagaga agctccgaca gaaagacaaa catccgcagt tgttcgtcgg gcatcaggta
     2521 ttgttcctcg tgcttcgacg gagaagaatc gacgcgcccc agaggatgaa gatcaccggc
     2581 caccagcggt ggagcgacac cgacaccact cccagatttt tcaaaagaaa aatgccaccc
     2641 agcacaatca aaatcagcgg aaaccagatt tcaccgccgc gtctgcccac ccagacctcc
     2701 tttgttaaga ggtaagatag ggccacagaa atcgcggtca atggtttttc tcgctggtag
     2761 cataggtcct cgtggccgtt ttttttagaa aaacggggta tgcccctccg ctatccggtt
     2821 cattgttatg gaattgtggt acaaaccttc tatgaacgcg atcgtattga cccctccctc
     2881 accctcctcg gtacgagtct tcgaccctca agggagggaa gccctttacc ttctctccgt
     2941 aaggctgatt actccctctc ctgtagtggt tcgatccgct tcaggcggac tcaccaccct
     3001 gaaattgtcg agggagaggg ataaagggtg aggtctctac cctgacactc gaatgtgatt
     3061 cgaataaatc catcccctcc ccttccggta cgcctcaatc cggtagaccc cagataattt
     3121 tgcttcatag tgcaacacca tcccctgcgc ggtggtggcc agttgtccat tgtgaattaa
     3181 acgcgattcg cagggccggg ggaagtgcgc gtgcaagtgc accgacttct ttaacgaaat
     3241 ggtctctcct attgtgacct cgtccccccc attctccgcc caaaagcgaa agttcatcgc
     3301 ctccccccac cggtagttcg aaatgaacac ccggcagtgc cggatggctt ccagaagttg
     3361 tcttttggcc tccgggaatt cttttgaaag cggtttgtcg agtaaaacgt gggtgcggat
     3421 tgacttgaac tgcacctcgt aggggaaaat gataatggtg aacggcccga atttgtgcgg
     3481 gtgcgcatga acgtcaatgc cgccggtgcc caccgtcgga cgcttttggt tatattcatc
     3541 ccagcgcgct aagatgcgcg gcgtagggcc gtagagcgag cgccgtggtc gaaagaccat
     3601 cgccagccgg ttccagcggg tcaacttctc catccactcc gacatctggt tccagatttc
     3661 aatcccgtcg aagccgtcga catcccaggc gttccagggg tagggccggt actgcggcag
     3721 tttattgcgc acctcgtccg ggtgagcgat aatccccaga ccgccgtgct cgcgcacttt
     3781 ccggacgtac tccttcggct tcaaatcggc cggcagcacc tgcctgagat taaatgccag
     3841 caggtgattg cgatcttctc tatcattgat ttcatatccg atcaatacta aggttcggtc
     3901 gtaccagccc tctttttttt cctccagccc tttcagggtc atgtgatcgg agaaaagcat
     3961 gaagtccaac cccgcgttcc ggccgatggc aatcacttcc gcaatcggct tgctgccatc
     4021 cgagtaagtg gtgtggatgt ggatggctcc gacgtagtga tagagtggtt tcattgatgg
     4081 ggttcgactg ataatttcac ccgtttctgg aaacggtccg gcactttgcg gaacaggatc
     4141 aggccgacag ccataagcat tgccagcgcg acgatggcaa tccggtactg gatggagggc
     4201 agcacctgcg gcgaaaaggt gacgcccaga cgttccaggc cggaaatcat tgcctgcacg
     4261 atagccgaat ccactttgaa atacaagacg cagaagcccc agaccagagg acctaaaatc
     4321 gccgccgccc ggcccgaaag ggcgtacaat ccgaagaatt gccctacccg cttggccggc
     4381 acgagactgg ccaaaagcgg ccgggccgaa gtccaggtcg aaccgagaaa aatgccgatt
     4441 gctcccgcca gtatccagag cacggtgaga ttccgcgaaa tcgcaatcag caggagcgag
     4501 acgatccagc cgaagaccac aaaccgcaga gttcgcttgg ggccgatgcg gtcaacaatc
     4561 agcccgcaga gaaacgaacc gcccaccgcg cagagcgtgg ctacggagag gaagatcgtc
     4621 ttcttgacgt ccggcagacc caccactttc tccagataca cagccatata aacaatggtg
     4681 gtagcgatgg catcctcaaa gagaaaatcc gcgaccaaaa agcgcaccac acccggatac
     4741 ttgcggctgt ctaccagccc gtttttcacc ttgcggaagg cggccaggat atagagccgg
     4801 gaaaggcgta ccgcccggcc tgccttctcc cgcacgaaaa agaagatggg gagcgcaaag
     4861 aggaaaaaga agacggcggt ggggaaaaaa gaggccgccc ggcccctttc gggctggccg
     4921 aacaggtggc ccaggtgcac ctttccttcc acgaatggcc ggactaagag catgccggcg
     4981 atggagccga cgtaacccaa agccacgcca atgcccgaaa tcttgccaat atttgtagaa
     5041 gtcgaaatga acggcagaag cgagttgtag aagacgttgc ccccttcgta gaagtagttt
     5101 gccaaaacga aaagccccag tgccaaaagc accgttccat tcccgggtcc cggacggcgg
     5161 ctcacccagc caatgagcaa ggtcaaaagg caacaggcca gcgtccacca gaaaagaaag
     5221 ggcagcttct tccgggcatg gtcggagacc gcgcccaaaa tcggcatcgt caccgctaca
     5281 aagaccatcg aaagcgaata ggcggcactg taccagatgt cttctttccc ttgatcgaca
     5341 attacccatt gggaaaagta gagactgata atgttcattg agaaaattgt gttagcaaag
     5401 tcgtaagcag cccagccgaa gatcgtcaaa cggtcgcgtc cggacatgcg cttaccaggt
     5461 agtcgcccag aggccgaagg cgatccccac gccgtcggcc accaggtctt tcagactgaa
     5521 gcgcgtcctg cggacggtcc ggtcgtaggt ttccttggcg gttccgatga aaaatgtggt
     5581 ggtgaccgcc acgacccgcg cctccgactt tttttcatcg aaaatttccc gattgacctg
     5641 gtagatgaaa aacgtcaggt aggtgctgac cagcgcgtgc tggactttat ccccgccgaa
     5701 ccagcaatca gaagttttct tcttttggcc cggagaggcg tcggccgcct gcatagaagg
     5761 actgaagcgg gaaaacgtaa gttcgaaagt attcggtaga gaatagaaag aaggagacgg
     5821 ctgcagagcg ggagcaacgt ctcgaggcac ttggcccaac agaccgagca ccaccaggca
     5881 cgccgctccc atcaatcgaa aaactcccac gcgaaaccga aactatggcc gcgctcgact
     5941 gcaggcgtta tcccgcgcag ggtgtagggc cggttggtga ggttctcaaa gaagtagtat
     6001 atggtcaggt ctttgatttt gaaccgggct ttgaaatcga ggcgcaaaac agcgggaagc
     6061 cggttgcccg ggtacagatc atctttccgc gaggaaagga attcaccatc cagttccccc
     6121 tgcacggtca ggacattttc aatcggccgc tccgcgttcc acacctggaa at
//
LOCUS       DBNGZD010000047         1116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_631184, whole genome shotgun sequence.
ACCESSION   DBNGZD010000047 DBNGZD010000000
VERSION     DBNGZD010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_631184"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="AC16GM_01350"
     CDS             complement(<1..822)
                     /locus_tag="AC16GM_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922352.1"
                     /translation="MKLLLLFLLLFTTLLGFTSDYLLLKINQTWNRSQPGRQAVLEEW
                     LSTHWERQPPQNSRDLQQLVRDLVVFTGPAEVTFIPGKDSLLPPLRFPDPPLAGGNKG
                     DQIVFTLPGKQGSLTLWLPANVAGVPVGLVHGLWLLKIIAVGLLLVFLPLLWRYIFAP
                     LKTISTQVQALPVPVPPRHPKSDAEVAIASFQQMIAELKTQQAELAALNALLHDRSHR
                     LAKYNDQILRQMNLIAVICDAEGTVTHWNHRAESTFDLAANRVIDQPFQQALRSVP"
BASE COUNT          241 a          305 c          351 g          219 t
ORIGIN      
        1 gggaacactg cgcagggcct gttggaaggg ctgatcaatg acccgattcg cggccaggtc
       61 gaaggtgctc tcggcccggt gattccagtg ggtaaccgtc ccttccgcat cgcagattac
      121 cgctatcaaa ttcatctggc gcagaatttg atcattgtac ttcgccagac ggtgcgaccg
      181 gtcatgcagg agggcgttga gcgccgccag ttccgcctgc tgggtcttga gttccgcaat
      241 catttgctgg aaactggcga tggcgacttc ggcatcactc ttggggtgcc ggggaggcac
      301 gggcaccggc agcgcctgca cctgggtaga aatcgttttc agcggcgcaa aaatgtaccg
      361 ccacagaagc gggagaaaga ctaaaaggag gccgacggca atgattttca gaagccagag
      421 cccgtggacc aatcccaccg gaacgccagc gacgttagcc ggcaaccaga gtgtcagcga
      481 cccctgtttg cccgggaggg tgaagacgat ctggtctcct ttattcccac cggccagcgg
      541 cgggtcgggg aagcggaggg gcgggaggag cgaatctttc ccggggatga aagtgacctc
      601 tgcgggaccg gtgaaaacaa ctaagtcacg taccaactgc tggagatcgc ggctgttctg
      661 cggcggctgg cgctcccagt gggtggagag ccattcctcc agaaccgcct gacgtcccgg
      721 ctgcgaccgg ttccaggtct ggttaatttt gagcaggagg tagtcgctgg taaaccccag
      781 gagggtagta aagagcaaaa ggaacagaag cagcagcttc acttccgctt agtacgacac
      841 gcgaccgaag cggcccggca gttggttcaa cagttgtttc aatggtagca ggagacgttg
      901 gcccatgggt cgatcactac tttcacgtat ggtctctcag cccgtgaaac cggcagggtg
      961 atggttaaag ggtgatctgg cctgcgccgc caccctatcc gataccaatc cagaacacct
     1021 cttttgcaac cacgatgccg ggggctcagg tgtgggctgt tcaggttgtc agttcgaacc
     1081 tcaagggaat tgacaagttc aacggcttcc cggtca
//
LOCUS       DBNGZD010000048         4215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_631203, whole genome shotgun sequence.
ACCESSION   DBNGZD010000048 DBNGZD010000000
VERSION     DBNGZD010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_631203"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="AC16GM_01355"
     CDS             <1..339
                     /locus_tag="AC16GM_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922353.1"
                     /translation="KAIGHKRPRDAFVHQKLGVAYAGLGRKEDAIREGKKALELRPMT
                     RDARFGANLVEGLAEIYVMVGEYDLAIDQLEYLLSVPSLISVPFLRLDPAWIPLRSHP
                     RFQKLINERA"
     gene            complement(383..1045)
                     /locus_tag="AC16GM_01360"
     CDS             complement(383..1045)
                     /locus_tag="AC16GM_01360"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR2922354.1"
                     /translation="MEKKIKIPRPKVRQEWGRDPVTKVKSSEKLYRREELKKIDWEAE
                     REPGEWEMAAGQFVFCPKCSAELVGRRLEGRMRLVCPVAACGFIYYRNPVPAVGAIIL
                     QDGKLLMVKRKFEPQAGKWSLPAGFIEYEESPTACVRREVKEETGFTIEIDRLFNVYP
                     AQDDPRSQVVLIIYLARIVSGRLKPGDDAVKAEFFAPENIPEDVAFTAHRKAIAEFFQ
                     LW"
     gene            complement(1053..3296)
                     /locus_tag="AC16GM_01365"
     CDS             complement(1053..3296)
                     /locus_tag="AC16GM_01365"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR2922355.1"
                     /translation="MQGAPVVAVVYLVCGIILFLLGLLILRENIRSRLNRTTGLMLFF
                     AALGALFAAFGTVLGPVDEKLLARPGVALVNNLFYFWELFFPQLLFFALVFPYERPFV
                     RRFPRLRLVFYLPYLFHVLLVSLFSDPTRLERWFSTENMAAPLRAILEPLAVAGKLLL
                     AFLSLLYQAHVQLFSVVNLVYVAASIWLLWRGYRRLTNPRLKAQVRLIFWGIRGGVGL
                     YALAFLLPALAPALSPGPRLRQLLTIGALVTGAGAVAWGIIRYQFMDVKLLVRQSLAY
                     SVTSALVVGLYVLSANQFGRLVKNIFGQKIPLLDVGFIALAIILFQPLFGRIDDLIKR
                     WFIRGTSDFRNLMERFTREIISILDPRELKQTVLEVLEKEVLVERAYLLLREREKPAY
                     LLFAGDPTSARETVLFAEDDPVIAHIGGKSKPFVAELAEPALRESPLGQFCQRERVRV
                     IVPLLEQKFLVGCLALSDKHSGYRYTAEDFTLLSVLANQLTVALANAELYREHLEKQR
                     LEEELALARQIQFDLLPRFNPRGPEFEFASFSQPSRQVGGDYYDYLLAESKVGVVIAD
                     ASGKGMPAALLVAMLQATLKAEYRHRKPLAEIVGNVNELVASSTSPEKFVSFFCGDFE
                     PEKKLFTYTNAGHNHPILLRADGSWEELTTGGLLLGVFPESRYEAGRVALRPADSLIF
                     YTDGLTDAQNQEEEQFGEERLRKLVREHRNLCAEELKDKIITTVNDFCAGAPQVDDMT
                     LVVLKVF"
     gene            complement(3306..3755)
                     /locus_tag="AC16GM_01370"
     CDS             complement(3306..3755)
                     /locus_tag="AC16GM_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2922356.1"
                     /translation="MPADRVIRKRDAIIIPSSQKYLRAVDDYLERKLRRAGLDRSLVA
                     DLAISVSEAVNNAIQHGNKGDPSKKVTVRCALENRRVTIEIEDQGAGFDPAALPNPLE
                     EANLLKEVGRGIFIVKKLVDELSFTPAVGGAGMVVRLVKFLSAAGGK"
     gene            complement(3873..>4215)
                     /locus_tag="AC16GM_01375"
     CDS             complement(3873..>4215)
                     /locus_tag="AC16GM_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR2922357.1"
                     /translation="WMKISSREQNGAVILEPKGSLMGGDETEALHGQVRDLVTNNKKK
                     VVINLAKVDWVNSAGLGALLSCLATARRNSGELKLANLTEKIQSLITITRLSTIFDTY
                     DSVEQAVASFK"
BASE COUNT          933 a         1299 c         1160 g          823 t
ORIGIN      
        1 aaagctatag gacacaaacg gcctcgggat gcttttgtcc atcagaaact tggcgtcgcc
       61 tatgccggat taggccgcaa ggaagatgcc attcgcgagg ggaaaaaggc cctggaactg
      121 cgaccaatga cgagagacgc ccgttttggg gcaaatttag tcgaaggctt ggccgaaata
      181 tacgtgatgg tgggcgagta tgatttggcc atagatcaat tggagtatct tttgtccgtt
      241 ccttctttga tttccgttcc gtttcttcgg ctcgaccccg cctggattcc cctgcgcagc
      301 catcctcgtt tccaaaaact cataaacgaa cgcgcttaaa aatcaaaaag gtcttccgca
      361 tactgtattt tgcttttttg ctttaccaca actgaaaaaa ctccgcaatc gccttccggt
      421 gcgcggtgaa ggcaacgtct tcggggatgt tttcgggagc aaaaaactcg gctttgactg
      481 cgtcatcccc cggctttaag cggccgctga cgatgcgcgc taagtagatg atcaaaacca
      541 cctggctgcg cgggtcgtcc tgcgccgggt agacgttgaa gagccggtca atttcgatag
      601 taaatccggt ttcctctttc acttcgcggc gcacgcaagc ggtgggagat tcctcatact
      661 cgataaatcc cgcggggagc gaccacttcc ccgcctgcgg ctcaaacttg cgcttgacca
      721 tgagcaattt gccgtcctgg agtatgatcg cccccaccgc cggcaccgga ttccggtagt
      781 aaatgaaacc gcaggcggcc accggacaga ccaaacgcat ccgcccctcc agccgccggc
      841 ccactaattc agctgaacac ttggggcaga agacaaactg ccccgccgcc atctcccatt
      901 cgcccggctc ccgctccgcc tcccagtcaa tctttttcaa ctcctcgcgg cggtaaagct
      961 tctccgacga tttgaccttg gtgaccgggt cgcgccccca ctcctggcgg acctttggcc
     1021 gagggatttt gattttcttt tccatctttt cctcaaaaca ccttaagcac gacgagcgtc
     1081 atgtcgtcca cctgcggcgc gccggcgcaa aagtcattga cggtcgtaat aattttatcc
     1141 ttcagctctt cggcgcacag gttccggtgc tcccgcacca atttgcggag gcgctcttcg
     1201 ccgaactgtt cctcctcttg attctgggcg tcggtcaagc cgtcggtgta aaaaatcaaa
     1261 ctatcggccg gacgcaaggc cacccggccc gcctcgtacc ggctttcggg gaaaacgccc
     1321 agaagcaggc ctccggtggt gagctcctcc cagctgccgt ccgcccgcag taagatcggg
     1381 tggttgtgcc cggcgttggt ataggtaaag agtttctttt ccggctcaaa atcaccgcag
     1441 aaaaaagaaa cgaacttttc cggtgaagtg ctggaagcca ctaactcatt gacgttgccc
     1501 actatttccg ccaatggctt ccggtgtcgg tactcggcct tgagcgtcgc ttggagcatc
     1561 gcgaccaaaa gcgccgccgg catccccttg cccgaggcgt cggcgatcac cacgcccact
     1621 ttgctctcgg ccagaaggta gtcgtagtag tcaccgccca cctggcgcga cggctggctg
     1681 aaactggcaa attcgaactc cggccccctc ggattgaatc tcggcaaaag atcgaactgg
     1741 atctgccggg ctaaagccag ctcttcttcc agacgctgtt tttccagatg ctcccggtac
     1801 agttcggcat tggccagggc cacggtcaac tggttggcca gaaccgaaag gagggtgaaa
     1861 tcttcggcgg tatagcggta gccggaatgc ttgtcgctta aagccaggca accgacgagg
     1921 aatttctgct ccaaaagcgg aacgatgacc cgcacccgct ctctctggca gaactggccc
     1981 aggggtgact cgcgcagggc cggttcggcc agttcggcca cgaagggctt tgatttcccg
     2041 ccaatatggg cgatgaccgg atcgtcctcg gcaaagagga ccgtttctct ggcggaagtg
     2101 ggatcgccag cgaacaacag gtacgcgggc ttctcccgct cccggagcaa gaggtacgcc
     2161 cgctcaacca acacctcttt ctccaatact tccaggacgg tttgcttcaa ttcacgcgga
     2221 tcgaggatcg aaataatttc acgagtgaag cgctccatca agttgcggaa gtcgctggtc
     2281 ccgcggatga accagcgctt gatcagatcg tcgatccggc cgaagagcgg ctggaagagg
     2341 ataatcgcca gcgcgatgaa gccgacgtcc aaaagcggaa ttttctgccc gaagatgttt
     2401 ttcaccagcc ggccgaattg attggccgaa aggacgtaga gccccaccac cagagccgag
     2461 gtgaccgagt aggctaggct ctggcggacc aagagcttca cgtccatgaa ctggtagcgg
     2521 atgatgcccc aggccaccgc accggcgccg gtgaccagag ccccgatggt caaaagctgc
     2581 cggagcctcg gaccgggcga cagggccggc gccagtgccg ggagcaaaaa ggccagagcg
     2641 tacagaccca ctccgccgcg gatcccccag aaaatcaggc gcacctgggc tttcaaccgg
     2701 ggattggtca aacggcggta accgcgccac aaaagccaga tcgacgccgc cacgtacact
     2761 aagttcacca ccgagaagag ctggacgtgc gcctggtaca aaagcgagag gaacgccaaa
     2821 aggagtttgc ccgccaccgc caaaggctcc aaaattgccc gcagcggagc agccatgttt
     2881 tcggtggaaa accagcgctc cagccgcgtc gggtcggaga agagactgac taagagaacg
     2941 tgaaagaggt aggggaggta gaacaccaag cggagccggg ggaagcgccg cacaaagggg
     3001 cgctcgtagg gaaaaacgag cgcgaagaac aacagttgcg gaaagaagag ttcccagaag
     3061 tagaagaggt tgttgaccaa agccaccccg ggccgcgcca aaagtttttc gtccaccgga
     3121 cccaggacgg taccgaaggc ggcaaagagc gcccccagcg cggcgaagaa gagcatgagg
     3181 ccggtggtgc gattgagccg gctccggatg ttctcgcgca gaatcaaaag ccctaaaagg
     3241 aaaagaatga tcccgcagac gaggtacacc accgccacca caggcgcacc ctgcattctt
     3301 cggcctcact tcccgccggc ggcggacaaa aacttcacca gccgcactac cattcccgcc
     3361 ccgccaacgg cgggggtaaa cgagagctca tccaccagct ttttaacgat gaaaatgccc
     3421 cgccccactt cctttaagag attcgcttct tccaggggat tcgggagggc cgccggatca
     3481 aaccccgctc cctgatcctc gatttcgatg gttacccggc ggttttccaa tgcacagcgg
     3541 accgtgacct ttttgctggg gtccccttta ttcccgtgct ggatggcgtt attgaccgcc
     3601 tcggagacgg agatggccag atcggccacg agactccggt ccagcccggc ccgccgcagt
     3661 ttgcgctcca aatagtcatc caccgcccgc aggtacttct gcgaactggg gatgatgatg
     3721 gcatcccgct tgcggatcac ccggtcagcg ggcatagctg cgcccgtcgc tgcaatggaa
     3781 aacgctttgc ctcagggaaa gagccatcga aataccagac aaaaaaaggg aggcgctcct
     3841 ccctcccgta ccgctacgcc gtggcctcgg ccttacttga aactggccac cgcttgctcc
     3901 accgaatcgt aggtgtcaaa gatggtcgaa agccgcgtta tggtgatcaa actctgtatc
     3961 ttttccgtca ggttggccag tttcaactcc ccgctgttgc gccgggccgt ggcgagacag
     4021 ctcaaaagcg ctcccagccc cgccgagttg acccagtcca ccttggccaa gttgatgacc
     4081 actttcttct tattattggt caccaggtcg cgcacctggc cgtgcagggc ctcggtctcg
     4141 tcgcccccca tcaggctccc tttcggctct aagatcaccg cgccgttctg ttcacgcgaa
     4201 gatattttca tccaa
//
LOCUS       DBNGZD010000049         2299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_979558, whole genome shotgun sequence.
ACCESSION   DBNGZD010000049 DBNGZD010000000
VERSION     DBNGZD010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_979558"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /gene="ribA"
                     /locus_tag="AC16GM_01380"
     CDS             <1..1024
                     /gene="ribA"
                     /locus_tag="AC16GM_01380"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR2922358.1"
                     /translation="MVAENTAKLETRFTVSVDALPNTQSGCSAFDRAQTIRLLADPQT
                     RPDDLARPGHIFPVVAAEGGVLKRPGHTEAAVDLARLAGLAPVGVICEIMNDAGKMAR
                     GRELFALAEKFSLKVVTIADLVTHRCKTEKLVQRVSTVDFPTRFGHFHLHLYRALVEK
                     QDHLAIEKGAVEGKSGVLVRVHSQCMTGDIFHSSRCDCGAQLEQALEQVEKEGSGVLV
                     YLCQEGRGIGLANKILAYSLQDQGQDTVEANVALGFEPDLRDYDVAAQILRDLGVQSV
                     RLVTNNPHKVADLKRYGVPVEQRLASITEPTAHNRRYLRTKKEKMGHWVEVEGRALTG
                     EPVKKK"
     gene            1021..1500
                     /gene="ribH"
                     /locus_tag="AC16GM_01385"
     CDS             1021..1500
                     /gene="ribH"
                     /locus_tag="AC16GM_01385"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR2922359.1"
                     /translation="MKEHKGQLSASGKKFAIVASRFNEFVTHQLVSGCLDCLTRHGAD
                     DQSIELFWVPGAFEIPPVAQRLATRKKYDAIICLGAVIRGGTPHFTFIAAAATNGIAA
                     VAQHSALPVVNGVLTTDNLEQAIERAGAKAGNKGWDAGLAAIELINLYAELEHGRKT"
     gene            1484..1918
                     /gene="nusB"
                     /locus_tag="AC16GM_01390"
     CDS             1484..1918
                     /gene="nusB"
                     /locus_tag="AC16GM_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR2922360.1"
                     /translation="MGARRKAREVALGCLYAAETATVAPQNVLTLHLAREKLSDKNRA
                     FVTVLFEQVRANTKTCDGLIARHLKHWDIARLSLIDRLVMHLAICEFLFFPDIPFRVT
                     INEAVELAKKFSTGDSGAFVNGVLDAIAKTIEQKTPAGTTAQ"
     gene            2074..>2299
                     /locus_tag="AC16GM_01395"
     CDS             2074..>2299
                     /locus_tag="AC16GM_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR2922361.1"
                     /translation="MIGALVFLASFIVFYLTKAPTLSFWDCGEFVACAAILGIPHPPG
                     SPLMVVLGRVFTLLPLNPDIAVRVNLISVVT"
BASE COUNT          477 a          630 c          717 g          475 t
ORIGIN      
        1 gatggtggcc gaaaacacgg cgaaattgga aacgcggttc accgtgtcgg tcgatgcact
       61 ccccaacacc cagagcggct gttcggcctt tgaccgggcg cagacgatcc ggctcttggc
      121 tgatccccaa acgaggccgg atgacttagc ccggccggga cacatttttc cggtggtcgc
      181 cgccgagggc ggcgtactca agcggcccgg gcatacggaa gcggcggtcg acctggcgcg
      241 tctggccggg ttggctccgg tgggggtcat ctgcgaaatc atgaatgatg ccgggaaaat
      301 ggcgcgcgga agagagcttt tcgctttggc cgaaaaattc agtttgaagg ttgtcacgat
      361 agccgatctg gtcacccacc gctgtaagac ggagaagttg gtccagaggg tctcgacggt
      421 tgacttcccc acccgtttcg ggcatttcca cctgcacctg taccgggcgc tggtggaaaa
      481 gcaggatcac ctggcgatag agaaaggcgc ggtagaggga aaaagcgggg tactggtgcg
      541 ggtgcattcg caatgcatga ccggcgacat cttccattcc agccgctgcg actgtggcgc
      601 acaactggag caggcgctgg aacaggttga aaaagagggc tcaggggttc tggtttatct
      661 ctgccaggag ggacgaggca tcgggctggc caataaaatt ttagcctaca gcctgcagga
      721 tcaggggcaa gacacggtgg aggccaacgt ggcgttgggt tttgaacccg acctccgcga
      781 ctacgatgtg gcggcgcaaa ttctccgcga cctgggagtg cagtcggtgc gtttagtcac
      841 aaacaacccg cacaaagtgg ccgacctcaa gcgctacggt gtgccggtgg agcagcggct
      901 ggcttcgatc acggagccga ccgcacacaa ccggcgctac ctcagaacca aaaaagaaaa
      961 gatgggccac tgggttgagg tggaaggccg cgccctgacc ggcgaaccgg tgaagaaaaa
     1021 gtgaaagagc acaaaggaca gctgagtgct tccggcaaaa agttcgcgat cgtcgccagt
     1081 cggttcaacg aatttgtaac gcaccagctc gtcagcggct gcctggactg cctcacccgg
     1141 cacggagccg acgaccagag tatcgagttg ttctgggtcc cgggagcttt tgagattcct
     1201 cccgtggcac aacgcttggc cacacgcaag aaatatgacg cgatcatctg tctcggggcg
     1261 gtcatccggg gcgggacgcc acatttcacg tttatcgcgg cggcggcgac caatggcata
     1321 gccgctgtgg cgcaacattc agctctaccg gtggttaatg gcgtcctgac cacagacaat
     1381 ttagagcagg cgattgagcg ggccggggcc aaagccggca acaaaggctg ggatgcgggg
     1441 ctggcggcaa ttgaactgat caacctctac gctgagctgg agcatgggcg caagacgtaa
     1501 agcgcgggaa gtggccttgg gttgcctcta cgcggcggag acggcgaccg tggcgcctca
     1561 gaacgttctc acgctccatt tggcccgcga gaagctttct gacaagaacc gggcttttgt
     1621 aaccgttctc tttgaacaag tgcgggccaa caccaaaacg tgcgacggct tgatcgcccg
     1681 acatctgaag cactgggata ttgcccgtct ttcgctcatc gaccggttgg tcatgcacct
     1741 ggcgatctgc gaatttctct tcttccccga catccccttc cgggtgacca taaacgaagc
     1801 agttgagttg gccaagaaat tttccacggg cgattcgggc gctttcgtca acggcgtctt
     1861 agacgccata gccaaaacca tagagcagaa gactccggcc ggaacgaccg ctcaatagct
     1921 actttttttc gagggggaaa tggcgttgag taagctccgt tcgggctgta tattcgccaa
     1981 actgtgggaa cgccagccgt cgtcatagcc cgttcaccta tcacactccc acttccggtt
     2041 cgtgatggga tctcttccaa atgggttgac cgtttgatcg gtgctttggt tttcctggcc
     2101 agttttattg ttttttacct gacgaaggcg ccgaccctgt cgttttggga ctgcggggag
     2161 tttgtggcct gcgcggcgat cttggggatt ccgcacccgc cagggtcgcc tttgatggtg
     2221 gttttggggc gggtgttcac gcttttgcct ctcaatccgg acattgcggt gcgggtgaat
     2281 ctcatctcgg ttgtgacct
//
LOCUS       DBNGZD010000050         9001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1256953, whole genome shotgun sequence.
ACCESSION   DBNGZD010000050 DBNGZD010000000
VERSION     DBNGZD010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1256953"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16GM_01400"
     CDS             complement(<1..271)
                     /locus_tag="AC16GM_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922362.1"
                     /translation="MRKTDVTNIIMEYREATRHIWNTYFVGKVKSLYECGILDYYEMI
                     DKNLFYAIVLEAINRGSYKIDYFRSQPIPFLRVAIREEIDRFSMMI"
     gene            complement(329..1054)
                     /locus_tag="AC16GM_01405"
     CDS             complement(329..1054)
                     /locus_tag="AC16GM_01405"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR2922363.1"
                     /translation="MKLEALTWQDFKRLVPSKIDTALLPVGTIEAHGVATLATDNLIP
                     ETLAQRMADKVKALILPTVPYGITRSLFGYPGSFTISPQNFTAYLVDIFRSIATQKFR
                     RLVVLNGHGGNNDALKQAALDINHTTGLKIAVIHWWTICQKVCREVYGEAGGHAALDE
                     TAFVLAVKPEYVKKNQYKKEMAYLMTEGGADIYPNPGSIILYEPGQGYLNFDPEKAQQ
                     YVEGCVKEVTRFVLDIFVRWKKI"
     gene            complement(1073..1804)
                     /locus_tag="AC16GM_01410"
     CDS             complement(1073..1804)
                     /locus_tag="AC16GM_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR2922364.1"
                     /translation="MRNRKEALVVFVRAPHYGELKARLAQELAPAEVESLYRAFVGDT
                     VMLCSRVNDAQLVVGFTPPEARREVTGFVEEALLAANHQKKPVPGPLVFSEQTGANLG
                     ERLRAMFALLFRQGYHEVVIIGADSPTVPPETLRKAFVLLKKKPVVLGPTLDGGYYLV
                     GLARPAPEIFDRIAWGTDQVYPQTVEKLKSLGLEWEELPVWYDVDTVDDLEFLVRDIN
                     QLRLAGDEESLAATEVVLEKILNKS"
     gene            complement(2075..2311)
                     /locus_tag="AC16GM_01415"
     CDS             complement(2075..2311)
                     /locus_tag="AC16GM_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR2922365.1"
                     /translation="MNCAQALSYLYEYVDRHLPAPLRTEVQAHIAICRGCTRRFGIEE
                     SLRAVVGRSGRVVAEIAGLKVALRQKLAAVRGKG"
     gene            complement(2308..2919)
                     /locus_tag="AC16GM_01420"
     CDS             complement(2308..2919)
                     /locus_tag="AC16GM_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR2922366.1"
                     /translation="MKDSSHPASLPAAPPDGKEFEQAVFAYVAPLYRSALRLTRNKSD
                     AQDLVQDTVLKAYRFFHRYQRGTNLKAWLFKIMTNLYINAYREKARKPQEVTIEDVEE
                     LYLYHRLTETRRPGEANPEETFFGQLYEDEVRRVLDELPADYRLVVVLAFVEEFSYEE
                     IAPITRLRLGTVKSRLFRGRKLLQKKLWDYAVRAGYLKRKREA"
     gene            complement(3093..3833)
                     /locus_tag="AC16GM_01425"
     CDS             complement(3093..3833)
                     /locus_tag="AC16GM_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR2922367.1"
                     /translation="MFQQEVSLLAAFGAGIISFVSPCVLPIIPGYLSFVSGVSVEELQ
                     GGENRGRLLKRVSLTVLFFILGFSAVFVALGASASAFGLFLATHRSLFNKIAGVIIFI
                     FGLHLLGILRIPFLNYEARFQARTRPLGLVGAFGVGLAFAFGWSPCLGPILGAILGIA
                     ANQETVGQGMLLLSSYSLGLGVPFFLAAIGFNSFLSVSRWVKKHFRAIEITSGAFLVL
                     VAVLIFTDQLAVIARVLLRWFPWLNEIG"
     gene            complement(3973..5331)
                     /locus_tag="AC16GM_01430"
     CDS             complement(3973..5331)
                     /locus_tag="AC16GM_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2922368.1"
                     /translation="MAKRILVAEGSSAIRSVAETVLRPEGYEVFTAEDGPLALRLLKA
                     LLPDLLVADWELPGFDGLALYEQAREQFPQLTKRTLFLVRHPQLVPRDVTRYGVGILT
                     KPFSPRDLLEAIDQLFRAAPTAAAPAPGGSGTTRTEAEPEQPDTDKLPPNRPAEPERG
                     AFAPPAHRVALHPPIPEPEEEEVSLEALLAEGGVELEVAGSKKTAQASDPNAAESEDP
                     TSKVAVVSPPGEPKARPLREPSREPAVSKKTPAQGWSMAGAVDLPDDLPKTSPPAYGA
                     PGDDAEIPHDYDWFLAEMERSAKGEKPRPGGVKPHDDAPTDRAYLKRPAESVTSKTVR
                     PEELKLKVEEVGTSRLGYERIVAEYRKELQKQAGEQRPGYGTAPPKAEGVKPVSTSTA
                     PRTLAPPEGTALLPGDFTDKLITEIGRQVAKEIMGRLDEDNLRALVKKQLANLSAALQ
                     KK"
     gene            complement(5354..6169)
                     /locus_tag="AC16GM_01435"
     CDS             complement(5354..6169)
                     /locus_tag="AC16GM_01435"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR2922369.1"
                     /translation="MPSPYATYRTPWSERITPMVQWLLIANTVIFLLQNAVGFRWMNY
                     RFGLVPLLIRENLMYWQFVTYLFLHGDLLHFLFNMFVLWMFGCEIERAWGSRQFLRYY
                     FLTGIGAGIFTFIFSFNSPIPTIGASGALFGIMVAYALLFPDRLIYVYFLFPVRAKYL
                     VLFFAGLEILASFNHTADGIGHFAHLGGMVVGYLYLKADWRWARFASAVKSWPGRRRL
                     AKIRRQMDKEARILEQVDAVLDRINQVGMENLTKEEKRLLEEASHLLHKTRST"
     gene            6611..7192
                     /locus_tag="AC16GM_01440"
     CDS             6611..7192
                     /locus_tag="AC16GM_01440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2922370.1"
                     /translation="MATYSFKPLSSNTWPDFARLVAKHNGVWGGCWCMSFHSEGVGTG
                     KAQSLKERRVRDGQAHAALVYDASTCVGWCQFGPTDELPRIKHRKAYVEGLTDLPHWR
                     ITCFFVDRDYRHKGVASAALTGALREIGRLGGGTVESYPEDVKGRSVSGSFLYNATVS
                     MFEQQGFRRVRRLGKNHWVVARRVRKASGKPPW"
     gene            7432..8025
                     /locus_tag="AC16GM_01445"
     CDS             7432..8025
                     /locus_tag="AC16GM_01445"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR2922371.1"
                     /translation="MRKIIVISMITLDGVMQAPGGPEEDTSGGFKYGGWVAPYFDEVG
                     GKVMKKQMKPADYLLGRKTFQIFASYWPEHADVWPGINDGTKYVMSKTMKKSDWRNSV
                     FLESLADIEKLKNSEGSDIQVWGSGKLIQTLLENDLVDELWLKIYPLTLGEGKKLFDN
                     GTIPAAFTLIESLVTPSGVIIANYRRAGKVKTGTIGA"
     gene            8048..8605
                     /locus_tag="AC16GM_01450"
     CDS             8048..8605
                     /locus_tag="AC16GM_01450"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR2922372.1"
                     /translation="MRKVVFAINITADGYCSHTDMIADEQLHEYFTRLLRNASLLLYG
                     RTTYQLMVPYWPEVAKNQSESIATNEFARVFDSLDKVVFSTTVKHVEGNSTRIVRSGV
                     AEEVLALKQRPGKDIAVGSLSIASQLSERGLIDEYYFVVHPVVAGKGPRLFETVKLQE
                     RLQLEFIGSETFQSGVVALHYRKRT"
     gene            8703..>9001
                     /locus_tag="AC16GM_01455"
     CDS             8703..>9001
                     /locus_tag="AC16GM_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2922373.1"
                     /translation="MKRVTGIGGIFFKAKDAPALQAWYKRHLGIDVQAWGGAAFDWTD
                     AEGKPVAGTTAWLIDPLESNHFAPSSAPFMVNYRVEDLHALVKVLKEEGCNVLEK"
BASE COUNT         2031 a         2524 c         2560 g         1886 t
ORIGIN      
        1 tgatcatcat actgaatctg tcaatctctt cccttatggc aactcttaga aaaggaatgg
       61 gttgagacct gaaataatca attttatagg aacccctatt gattgcctca agcacaattg
      121 cataaaagag attcttgtca atcatctcat aataatcgag aataccacac tcatataagg
      181 atttcacttt tcctacaaaa taggtattcc aaatatgacg cgttgcctct cggtattcca
      241 taataatatt tgttacatct gttttcctca ttttgacctg tagtttaggg agctggtaat
      301 tctttccgca gtcagatgct atgaaaactc agattttctt ccaccggaca aaaatatcta
      361 atacgaaccg cgttacttct ttcacgcatc cttcaacgta ttgttgcgcc ttctctgggt
      421 caaaattcag atacccctgc ccgggttcgt agagaatgat cgagccggga ttcggataaa
      481 tgtccgcccc accttctgtc atcaaatagg ccatttcttt tttgtactga ttcttcttca
      541 catactccgg cttgaccgcc aggacaaagg cggtttcatc caacgcggcg tgcccgccgg
      601 cttcgccgta tacttcgcgg cagactttct gacagatggt ccaccagtgg atgaccgcga
      661 tcttgaggcc ggtggtatgg ttgatgtcca gcgccgcttg tttcagcgcg tcgttattcc
      721 cgccgtggcc gttgagcacc accagccggc gaaatttctg ggtggcgatg gagcgaaaaa
      781 tatcgacgag gtacgcggta aaattctggg gcgaaatggt gaaggagccc gggtagccga
      841 aaagtgaacg ggtgataccg tagggaacag tgggcagaat gagggctttg actttgtccg
      901 ccatccgctg ggccaaagtt tccggaatga gattatcggt ggcgagcgtt gccacgccgt
      961 gggcttcgat ggtacctacc ggtaaaaggg cggtatcaat cttcgacgga acgagacgtt
     1021 taaagtcctg ccaagtgagc gcttctaatt tcacggtgtg aagggagggc tgttaagact
     1081 tgttcagaat cttttccaaa accacttcgg tcgctgccag cgattcttcg tcaccggcca
     1141 gacgcaactg gttgatgtcg cgcaccaaga attcgagatc atctaccgta tccacgtcgt
     1201 accacaccgg cagctcttcc cattccagcc ccaaggactt caatttttcc accgtctgcg
     1261 ggtagacctg gtcggtgccc caggcaattc tgtcgaagat ttccggagcc ggccgggcga
     1321 ggccgacgag gtagtagccg ccgtccagcg tggggcccaa aaccaccggc ttctttttca
     1381 acagcacaaa ggccttccgc agcgtctccg gcggcacggt ggggctgtcg gcgccgatga
     1441 tcaccacctc gtggtacccc tgccggaaga gcaacgcgaa catggcgcgc agcctttccc
     1501 ctaagtttgc cccggtctgc tcggagaaaa ccaaaggccc gggcaccggc ttcttctggt
     1561 gattggctgc caggagcgcc tcttcgacga aaccggtcac ttcccgccgc gcctccggcg
     1621 gcgtgaatcc caccaccagc tgcgcgtcgt tcactcgaga gcagagcatg accgtgtcac
     1681 cgacgaaggc gcggtagaga ctctccacct cggcgggggc cagttcctgg gccaagcgcg
     1741 ctttcagttc gccgtagtgc ggcgctctga cgaaaacgac caaggcctct ttgcgattcc
     1801 tcatactgcg gggcaacttt ttcctacgcc atcgttctgg tgctggtgag cacgatcgac
     1861 tcctgcggac accaccggct tctggccttg aagtataaag cccggaaacg atttgtcaac
     1921 gcgggtttgc gcctgagaaa gaaaaaactc aaagggggtt aaaaaaaatt gggggaagga
     1981 cagactcatt aagaaatgga gcgaatatgc ttgcgtcagc ccggttttga tccctacgca
     2041 ccggggggcg cgagggacca ctgccgtacg tcgcttagcc ttttcccctc acagcggcca
     2101 gcttttgacg cagtgctact ttcagtcccg ctatttctgc taccactctg ccgctgcgcc
     2161 ccaccaccgc ccgcaggctt tcttcaatcc cgaagcggcg ggtgcagccc cggcagatgg
     2221 cgatgtgcgc ctgcacctcg gtccgcagag gcgccggcag gtgccggtcc acgtactcgt
     2281 agagatagga cagtgcctga gcgcagttca tgcttcgcgt ttcctcttca aatagccggc
     2341 gcgcacggcg tagtcccaca gttttttctg caacagtttc cgcccgcgga aaagccgcga
     2401 cttcaccgtc cccagccgca gccgggtgat cggcgcgatc tcctcgtacg aaaactcttc
     2461 cacaaacgcc agcaccacca ccagccggta atcggcgggc aattcatcca gcacccggcg
     2521 gacctcgtct tcgtacagct ggccgaagaa agtttcctcc gggttcgcct cacccggccg
     2581 gcgggtctcg gtcaaccggt ggtagaggta cagctcctcc acatcttcta tcgtcacttc
     2641 ctgcggcttc cgggccttct cccggtaggc gttgatgtac aggttggtca taattttgaa
     2701 gagccacgcc ttgagattgg tgccgcgttg gtagcggtgg aagaagcggt aggctttgag
     2761 taccgtgtcc tgcaccaggt cctgcgcatc gcttttgttg cgcgtcagcc gcaaagcgct
     2821 ccggtagagc ggtgcgacgt aggcgaagac cgcctgctcg aactcttttc cgtcgggcgg
     2881 tgccgcaggg agagacgcgg ggtggctgga gtctttcatg gtacgctgtt atctctccgc
     2941 ctccgctccg ctctgggccg gggccacggc ccgcgccatt tcctacaacc ggagccgaaa
     3001 atgaaaagtt cccctcccgc ctgcggagcg gggaaccgga aaaagcgctc ctcggtacga
     3061 gtcgatcccg accggagcag gttcaatggt atttatccga tctcgttcag ccaggggaac
     3121 cagcgcagga ggacccgggc gatgaccgcc agctggtcgg taaagatgag caccgccacc
     3181 agaaccagaa aggcgccgct ggtgatttca atcgcccgaa aatgcttttt cacccagcgc
     3241 gacaccgaga gaaacgagtt gaagccgatg gccgccagaa agaagggcac gcccagcccc
     3301 agagagtaac tcgataagag gagcattccc tgccccaccg tctcctgatt ggcggcaatg
     3361 cccaggatag cccccaggat cggccccagg cagggtgacc aaccgaaggc gaaagccaat
     3421 cccacgccaa aagcgccgac caatcccaac ggccgggtgc gggcctggaa gcgggcttcg
     3481 tagttcaaaa acggaatccg cagaattccc aacaggtgca gaccgaagat gaaaatgatc
     3541 accccggcga tcttgttgaa gagcgaccgg tgcgtggcca ggaacagtcc gaaggccgag
     3601 gccgatgcgc ctaaagcgac gaacaccgcc gagaacccca ggatgaaaaa gaggacggtg
     3661 aggctcaccc gcttcaggag ccgtccccgg ttctccccac cctgcagctc ctccaccgat
     3721 acgccggaga caaacgaaag gtacccgggg atgatgggca ggacgcaggg cgaaacgaag
     3781 gaaatgattc ccgcgccgaa ggcggccaat agcgaaactt cctgttgaaa catctctgct
     3841 ccttgtccgc cgggcaggct tcggccggcc cggctttagg aaatgaacat acaacgctgc
     3901 aaccggtatc aacaatacga aaaggaaaac gacgaaaaga ggagatttta cggtccggaa
     3961 gaactgcggg ggctactttt tctgaagcgc cgcgctcagg ttggccagtt gttttttgac
     4021 caaggcgcgc agattgtctt cgtccaaccg gcccataatt tctttcgcca cctggcggcc
     4081 gatttcggta atgagcttgt cggtgaaatc accggggagc aaggctgttc cttccggggg
     4141 cgccaaggtg cgcggagcgg tcgaagtgga gaccggcttt accccttccg ctttgggggg
     4201 cgcggtgccg tagccgggac gctgctcccc ggcctgcttc tggagttcct tgcggtattc
     4261 ggcaacgatc cgctcgtacc ccaagcggct ggtgcccacc tcttccactt tcagcttcaa
     4321 ttcttccggc cgaaccgtct tcgaggtgac cgactcagcc ggccttttca gatacgcccg
     4381 atcggtgggg gcgtcatcgt gcggtttcac tccgccggga cgcggcttct cccccttggc
     4441 gctccgttcc atctcggcca aaaaccagtc gtagtcgtgg ggaatctccg catcgtcgcc
     4501 cggcgccccg taggccggcg ggctggtctt gggcagatcg tccggcagat caacggctcc
     4561 ggccatagac cacccctgtg ccggagtctt cttggagacc gccggttccc ggctcggctc
     4621 ccgcaggggg cgggctttgg gttctcccgg aggagacacg acggccacct tgctcgtagg
     4681 atcttcactt tccgctgcat tgggatcact ggcctgcgct gttttcttcg acccggccac
     4741 ttccagttcc acccctccct cggctaagag cgcctcgagg gaaacctcct cttcctccgg
     4801 ctcgggaatc ggtgggtgga gcgcgaccct gtgcgccggc ggagcgaaag ctccgcgttc
     4861 cggctcagcc ggacggttcg gaggcagttt atcggtatcc ggttgctccg gttcagcctc
     4921 ggtccgagtc gtacccgacc cgcccggggc gggagcagct gccgtgggtg cggcacggaa
     4981 cagttggtca atggcttcca gaaggtcgcg ggggctgaac ggcttggtga gaataccgac
     5041 tccgtagcga gtcacatctc gcggcaccag ttgtggatgc cgcaccagaa agagggtacg
     5101 cttggtcagc tggggaaact gctcccgcgc ctgctcgtac agggcaagac catcgaaacc
     5161 gggaagctcc caatcggcga ccaacaggtc gggcaggagt gctttgagca gccgcaatgc
     5221 cagaggcccg tcttcggcgg tgaacacttc atacccttcg ggccgcagga cggtctcggc
     5281 tacgctgcgg atggcgctgg agccctcggc gactaaaatg cgcttggcca cggcaatctc
     5341 cgtttcgtca acatcaggta ctgcgcgtct tgtgcaagag gtgactcgcc tcttccagaa
     5401 ggcgcttctc ctctttggtc aggttctcca ttcccacctg gttgatgcga tccagcacag
     5461 cgtcaacctg ttccagtatc ctggcttcct tatccatttg tcggcggatt ttggctaatc
     5521 ttctgcgccc cggccaactt tttacggcac tggcgaacct ggcccagcgc cagtcggcct
     5581 tcaggtagag gtagcccacc accatcccgc ctaagtgcgc aaagtgcccg atgccgtcgg
     5641 cggtgtggtt gaaggaggcc agaatctcca gtcccgcaaa gaacagcacc agatacttgg
     5701 cccgcaccgg gaagaggaag taaacgtaga tgagccggtc cgggaacaag agggcatagg
     5761 cgaccatgat cccaaaaaga gcgccggagg cccctatggt gggaatcggc gaattgaagg
     5821 aaaaaatgaa ggtgaaaatg ccggccccga tgccggtgag aaagtagtag cgcaggaact
     5881 ggcgcgaccc ccaggcccgt tcgatctcac aaccgaacat ccagagaacg aacatgttga
     5941 acaggaagtg caggaggtcg ccgtgcaaaa agaggtaggt gacgaactgc cagtacatca
     6001 ggttttcgcg aatcaaaagc ggcacgagac caaagcggta gttcatccag cggaagccga
     6061 cggcgttctg gaggaggaaa atgacggtat tggcgatcaa aagccattgg accatcggcg
     6121 tgatccgctc gctccagggc gtccgatatg tggcgtacgg ggaaggcata cctatccatt
     6181 taatatatcg gctgctgaag gagaaacaat ggtggtgaca gaagagaatg ggtcttgaca
     6241 gcggcgccga atcgggtaat tatggtagaa agttcggcca acttgccttg ccggaggata
     6301 aggaggaatg acccggcagg gggaatgctt gataagcagg agatagttca cagccacgtc
     6361 ctcaaccgcg gcccggctgg cgtgcgcagc ccgggcgaca gcctctgaga ccatcgagga
     6421 ggtttttatg ccgatccgca taatacgaca caataaaaca gccaaggagg ctcaggtgca
     6481 tgattctgaa agagatattg cttccgtcat tgtcgccaga acggtgtgtt atactgctga
     6541 aagccgaaat tctgcggctt atgcggatca atctaatcaa tgttgggcag ccagcgcgta
     6601 ggacaagacc gtggcgacgt acagtttcaa gccgctaagc tccaacacat ggcctgactt
     6661 cgcccgactc gtcgcgaagc acaacggcgt atggggcggc tgctggtgca tgtcgtttca
     6721 ctccgaaggc gtggggactg gcaaagccca atccctgaag gaacgccgag tccgagatgg
     6781 gcaagctcac gcggccttgg tctacgacgc ttccacctgc gtaggctggt gtcagttcgg
     6841 gcccaccgac gagcttcctc gcatcaaaca taggaaggcc tatgtcgaag gtctcaccga
     6901 ccttccacac tggcgcatca cctgtttctt cgtcgatcgg gactatcggc acaaaggcgt
     6961 cgcatccgcc gccctcacgg gggctttgcg agaaatcggc cgtcttgggg gaggaaccgt
     7021 agaaagctat cctgaggatg tgaaaggccg ctccgtctcg ggatccttcc tctataacgc
     7081 aacggtgtca atgttcgagc aacaaggatt ccgccgggtc cggcgtctag ggaagaacca
     7141 ttgggtggtt gccagaaggg tgcgcaaggc gtctggcaag ccaccgtggt gagcggctag
     7201 tcgtaggctg cccaacaact cgttggagcc gacccggcca gcccggtgtt tcgctttgac
     7261 gcgatactag gcttgggctg gccggacggc tcaactcgat gccgttatac ggagggaaaa
     7321 aatgttctct gcttcttcaa gcacgatctt tgttggcgag atttgtatcg taacaaaacc
     7381 aattttgaag aagcttttta aagaataaat tataagaaga ggttaaataa tatgagaaaa
     7441 ataatcgtca tatcaatgat tacattagat ggagtcatgc aagcacctgg tggacctgag
     7501 gaagatacat caggcggttt caaatatggc ggttgggttg cgccttattt tgacgaagtt
     7561 ggtggcaagg tcatgaaaaa acagatgaaa cctgcagatt atcttttggg cagaaaaaca
     7621 tttcagattt ttgcctccta ctggcctgaa catgcagacg tttggcctgg tatcaatgat
     7681 ggcacaaagt acgttatgtc caagaccatg aaaaagtcag attggagaaa ctcagtattc
     7741 ctcgaaagtt tggcagatat cgaaaagctc aaaaattcag aaggttctga cattcaagtt
     7801 tggggtagtg gtaagctcat ccagacacta ctcgaaaatg atttagttga cgaactctgg
     7861 ctcaaaattt accccttgac tcttggtgaa ggaaaaaagt tgtttgacaa tggcacaatt
     7921 ccggcagcat ttacattaat agaaagtttg gttacaccaa gtggagtgat tattgccaat
     7981 tacaggcgag ctggaaaagt taagacaggt actattggag cttaagaaga aataatgtga
     8041 ggagcttatg agaaaggtcg tttttgcgat caacattaca gcagacgggt actgcagcca
     8101 cacggacatg atcgccgacg aacagttgca cgagtacttc accagactgc tgcgcaatgc
     8161 gagcctcctc ctctacggcc ggaccacgta tcaactcatg gtcccctatt ggccggaagt
     8221 cgccaagaac caatctgagt caatagcaac caatgagttc gctcgcgtgt tcgactcgct
     8281 cgacaaggtc gtattctcta cgacagtgaa acacgttgag ggaaacagca cgagaatcgt
     8341 acgctcaggc gttgcggaag aggtgctcgc gctgaagcag cggcccggaa aagacattgc
     8401 cgtaggaagt ttgagcattg cttcccagct ttcggagcgc ggtctgatcg acgagtatta
     8461 tttcgtggtt cacccggttg ttgccggaaa aggaccgcgg ttattcgaaa ccgtgaaact
     8521 gcaggagaga cttcagctgg aatttattgg ctcggaaacg tttcaaagcg gcgtcgttgc
     8581 ccttcactac agaaagcgta cgtgagtaga aacgcgttga tcgttcgggc gggggcgggg
     8641 ctcccatctg ttaggccgca gaagaaacat tcctctcacc accaccgcgc atcggaggtc
     8701 acatgaaacg agtcactggc atcggcggca tcttcttcaa ggccaaagac gctccggcac
     8761 tgcaggcttg gtacaagcgg cacctgggaa tcgatgtcca agcctggggt ggagctgcct
     8821 tcgattggac cgacgccgag ggcaagccgg ttgcaggcac aactgcctgg ttaatcgatc
     8881 cgctagaaag taatcacttt gctccgagct ctgctccctt catggtcaac taccgagtgg
     8941 aagacctcca cgccttggtc aaggtcttga aggaagaagg ctgcaatgtg ctcgagaaga
     9001 t
//
LOCUS       DBNGZD010000051         1335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_840640, whole genome shotgun sequence.
ACCESSION   DBNGZD010000051 DBNGZD010000000
VERSION     DBNGZD010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_840640"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1335)
                     /gene="rpoB"
                     /locus_tag="AC16GM_01460"
     CDS             complement(<1..>1335)
                     /gene="rpoB"
                     /locus_tag="AC16GM_01460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843611.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR2922374.1"
                     /translation="AERIVIIPSGRGREGELDFSEADEYELDKFRRSNQDTCINQRPL
                     VQVGDRVEKGQIIADGPATDRGELALGANVLVAFLPWRGYNFEDAIVISERLVVDDIF
                     TSIHIEEFELQVRDTKRGAEELTREIPNVSEEAVMNLDERGIVRTGAEVEAGDILVGK
                     VTPKGETELSPEERLLRAIFGEKAGDVRDASLKAPPGMKGVVIDTRVISRKERTDAVK
                     KQEKTEIARIRKQFHQQMEQIRVIRDRRVLEILEGEVSRTLRNKRGDVVLRAGTKFKE
                     DTFTDIDVADLVGEEGFTDSDAKNRRVAQIVLHCEKLLEKKKGQLEVETEKVIRGAEL
                     PPGVVQLVKVRVATKRKVSVGDKMAGRHGNKGVVARTVPVEDMPYLPDGTPVDIVLNP
                     LGVPSRMNVGQILETHLGWAAQKLGLYVASPVFDGARSEDIAEALKKAGLPT"
BASE COUNT          203 a          419 c          406 g          307 t
ORIGIN      
        1 ttggtcggca ggccggcttt tttgagcgcc tcggcaatgt cttccgagcg ggcgccgtcg
       61 aagacgggac tggccacgta gagacccaat ttctgggcgg cccagcccag gtgggtttcc
      121 agaatctggc ccacgttcat gcgggaaggt actcctaagg gattgaggac gatgtccacc
      181 ggcgtgccgt cgggcaggta gggcatatct tccaccggga cggttctggc cactaccccc
      241 ttattcccgt ggcggccggc catcttgtcg ccgaccgaaa ccttgcgttt tgtcgccacg
      301 cgaactttga ctaactggac cacgcccggc ggcagttcgg cgccccggat cactttttcg
      361 gtttcgactt cgagttgtcc ctttttcttt tccagcaatt tttcgcagtg caggacgatc
      421 tgcgccaccc ggcggttctt ggcgtcgctg tcggtgaacc cttcctcgcc caccaggtcg
      481 gctacgtcga tatcagtaaa ggtgtcttct ttgaacttgg tgccggcccg gagcaccacg
      541 tcgccgcgct tgttgcgcag ggtgcgggaa acttcgcctt ccaagatttc cagaacgcgc
      601 cggtcgcgga tgacgcgaat ctgctccatc tgctggtgga actgcttgcg gatgcgcgcg
      661 atttcggtct tctcctgttt tttgaccgcg tcggtccgct ccttgcggga gatcacgcgg
      721 gtgtcgatga ccaccccttt catcccgggc ggcgctttga gggatgcgtc ccgcacgtcg
      781 cccgcttttt cgccgaagat ggcccgcagg agccgctcct ccggcgagag ctcggtttcc
      841 cctttggggg tgaccttgcc caccaggatg tcgccggctt ccacctcggc gccggtccgc
      901 acgatgccgc gttcgtccag attcatgacc gcttcttcgg agacgttggg aatctcgcgg
      961 gtcagttcct cggcgccgcg tttggtatcg cggacctgca gttcgaattc ttcaatgtga
     1021 atcgaggtga agatgtcgtc caccaccagg cgttccgaaa tgacgatagc gtcttcgaag
     1081 ttgtagcccc gccagggcag gaaagccacg agcacgttgg cgcccaaggc cagttcaccg
     1141 cggtcggtgg ccgggccgtc ggcgatgatc tgccccttct ccacccggtc ccccacctgc
     1201 actaaaggac gctggttgat gcaggtgtcc tggttggagc ggcggaattt atctaattcg
     1261 tattcgtcgg cttcggagaa atcgagttcc ccctcccggc cgcggccgga gggaatgatg
     1321 acaatgcgtt cggcg
//
LOCUS       DBNGZD010000052         3951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_979795, whole genome shotgun sequence.
ACCESSION   DBNGZD010000052 DBNGZD010000000
VERSION     DBNGZD010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_979795"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="AC16GM_01465"
     CDS             <1..1381
                     /locus_tag="AC16GM_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922375.1"
                     /translation="KLDRTIELLKRLATEQKLDALLKMAEKLLQGQKEVNAKTQASPA
                     DSLPQVSPDEKALEKQLDQLTQKASDLKRELQADPEFNIPQSAQFCDRPGQSGAKPKM
                     QKMQQALQQAQKKPSEQAGAEAQEEMESMFASMLQARQGISQCRKKGILGELRKALED
                     ALYLSESQEKLAADLNTSDASGRKLRQFAQEQNDLKTETERLSQRLEGTAKKSFFVPQ
                     PKNSPTYQAVARMQQAVTHLSNCSRAGAQQEQTEALYQLNQTAKELLSAIQNAAASSC
                     GSGGEELLQKMQNLSNMQLGINEQGMSLLPPNQPAQDQLASLQRLRAEQEVVKKSMEE
                     LQREYGSRKEILGRLDELGKEVEKVLEDFDRQNYGSQTLDRQKKILSRLLEYNQSLER
                     RDYTERRQAEFGEDIIRRSPQLSREQIEKAQRQSDTAFPFGDEPYPPEYEQVIKNYLK
                     AYGGEAPRK"
     gene            1390..3249
                     /locus_tag="AC16GM_01470"
     CDS             1390..3249
                     /locus_tag="AC16GM_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922376.1"
                     /translation="MLSRKTPRALLAGCVIVILCASAAPAQVSPDFQQNLNRVDQLLL
                     LNKFDEAVTLAEQLQKRYGDSSELLSRLKQAYLGAKRYADLITLLDREVARNPQDQET
                     LAQLASIYLKAGEKPKAEQTVKKLIALAPAEASVYSRAAGVYTENGYAREAIDWYGAG
                     RKALGNPRAFSWEVASLFEALRDYPAALREYFTQIDLEPTDAGLEARVTQLVNQVEAD
                     SSIERTLAEFTRWPGRPARGGEVPQPAGRVLAYRLYGDMLYNRGEIRLAVEEYRSADQ
                     YGGTRGTNLLRLILKLLADGYYAVADSACQALIAQIPNDPLVMQALLLQAQARTGEKR
                     YASAGDIYRQVVETTPLPREKAEALLAIALLHLEALNQPAVAQAEFQSLVTNYPDLSL
                     AQEARLRLGDCLLAQDKLDEALRYFQDFSRETNSAILGERLALRLAELFFYRGQVDSA
                     ADAYRLIVERYPRSTAVNDALNRLALLAFSGLAASPELSLFSRAEKLRTQRQFEKAIA
                     LYEKLADAGWQIDQVLWAQALALRAAGEYPKALVALAKLKTERLTSRWAACALKLSGE
                     IRLEDLGDPAGARAAFEEILKNYPDAIFTEEVRTRLKRLPPGSGGAESLRGGS"
     gene            complement(3252..3542)
                     /locus_tag="AC16GM_01475"
     CDS             complement(3252..3542)
                     /locus_tag="AC16GM_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922377.1"
                     /translation="MLAWCAIMFCLGVLAFMDSLFNYGEIFRQVNSVLFMLVSLGLLV
                     RTTTKIRARTVEGYIEKVSRLEEEIKQISATRKSASGGHDVRSERFVPSGSR"
     gene            3672..>3951
                     /locus_tag="AC16GM_01480"
     CDS             3672..>3951
                     /locus_tag="AC16GM_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR2922378.1"
                     /translation="MPGEFQNDTLKRFSHLDWLIGALVFLASFFVFYLTKAPSVSFWD
                     CGEFVACAAILGIPHPPGSPLMVVLGRVFTLLPLNPDIAVRVNLISVVT"
BASE COUNT          840 a         1246 c         1151 g          714 t
ORIGIN      
        1 caaactcgac cgcacgattg aactgttgaa acgcctggct accgagcaga agctggacgc
       61 gctcttgaaa atggccgaaa aacttctcca gggccaaaaa gaggtcaacg ccaaaaccca
      121 ggccagcccg gccgacagtc tgccccaggt ttcccccgat gaaaaagcgc tggaaaaaca
      181 gttggatcag ttgacccaga aggcctccga tctgaagcgg gaactgcagg cggaccccga
      241 gttcaacatt ccgcagtcgg cccagttctg cgaccggccc ggccagtcgg gcgccaaacc
      301 gaagatgcag aagatgcagc aggcgctcca gcaggcgcag aagaagcctt cggaacaggc
      361 gggtgccgaa gcgcaagaag agatggaatc gatgtttgcc tccatgctcc aggcgcggca
      421 gggaatttcc cagtgccgca agaaaggcat tctgggcgaa ctgaggaaag cgctggagga
      481 tgccctctac ctgtccgaaa gccaagaaaa gctggctgcc gacttgaaca cctccgacgc
      541 ctcgggccgg aagctgcgcc agtttgcgca agagcagaac gacctcaaaa ccgaaactga
      601 acgtctctcg cagcgcctgg aaggcaccgc caaaaagagt ttcttcgtgc cgcagccgaa
      661 aaattccccc acctatcagg cggtggcccg gatgcagcag gcggtcacgc acctctccaa
      721 ttgctcccgg gccggcgccc aacaggaaca aaccgaagcc ctctaccagt tgaaccagac
      781 cgccaaagag ctgctctccg ccatccagaa tgctgccgcc tcatcctgcg gctccggcgg
      841 cgaggaactg ctgcagaaga tgcagaacct gtccaatatg cagctgggga ttaacgagca
      901 ggggatgtcc cttctccctc ccaaccagcc cgcccaggac cagttggcct cgctgcagcg
      961 cctgcgcgcc gagcaggagg tggtgaagaa gagtatggaa gaactccagc gggagtacgg
     1021 cagccgcaaa gaaatcttag gccggctgga tgaactgggc aaggaagtcg aaaaggtgct
     1081 ggaagacttc gaccgtcaga actacggttc tcagactctg gatcgtcaga agaaaattct
     1141 ttcgcgtctc ctggagtaca accagtcgct ggagcggcgc gactataccg agcgccgcca
     1201 ggccgaattc ggtgaagaca tcatccgccg cagcccccag ctttcccgcg aacaaataga
     1261 aaaggcccaa cggcagagcg ataccgcctt cccgttcggg gatgagcctt acccgcccga
     1321 atacgagcag gtgatcaaaa actacctcaa ggcctacggc ggcgaggcac cccggaagta
     1381 gcccaaccta tgctttcccg aaaaactccc cgtgcgcttt tggccgggtg tgtgatcgtg
     1441 atcctgtgcg cttcggccgc gcctgctcag gtttcgcccg acttccagca gaacctcaac
     1501 cgggtagacc agctattgct gttgaacaag tttgacgagg ccgtcacgct tgcagaacag
     1561 ttgcaaaagc gctacggcga ctccagtgag ctcctctccc gtctcaaaca ggcctatctc
     1621 ggagcaaaac ggtacgccga cctgataacc cttttggatc gggaagtcgc acgcaatccc
     1681 caagaccagg aaacgctggc ccagctcgcg agcatctacc tgaaagcggg cgagaaaccc
     1741 aaagccgagc aaaccgtcaa aaaactcatc gcccttgccc ccgcagaggc ctcggtctac
     1801 agccgcgccg ctggggtata taccgaaaat ggctacgccc gggaggcgat cgactggtac
     1861 ggcgccggcc gcaaggcgct gggcaatccg cgcgccttct cctgggaagt ggcttcgctt
     1921 tttgaagcgc tgcgcgacta cccggccgcg ctgcgggagt acttcaccca gatcgatctg
     1981 gaaccgaccg atgccggtct ggaggcgcgc gtaacgcagt tggtcaacca ggtcgaagcc
     2041 gactccagta tcgagcggac cttagccgag tttacccgct ggccgggtcg ccccgccaga
     2101 ggcggggaag tcccgcagcc ggccggccgg gtcctggcct accggctgta cggggacatg
     2161 ctctacaacc gcggggagat ccggctggcc gtagaagagt atcgctcagc cgaccagtac
     2221 gggggcaccc ggggaaccaa tctcctgcgg ctcattttga aacttttggc cgacggttac
     2281 tacgctgtcg ctgattcggc ctgccaggcc ttgatcgccc agattccgaa cgaccccttg
     2341 gtcatgcagg cgctcctttt gcaagctcag gcccggacgg gcgagaaacg ctacgcgagc
     2401 gccggcgata tctaccgcca ggtggtggag acgacccccc tcccgcgcga aaaagccgaa
     2461 gcactcctgg ccattgcgct gttgcacctc gaagcgctga accagcccgc cgtcgctcaa
     2521 gcggagtttc aatcactcgt gaccaactat ccggaccttt ctttagccca ggaagcccgg
     2581 ctgcgtctgg gcgattgcct cctcgcccag gataaactgg atgaggccct gcgctacttt
     2641 caagactttt cccgcgagac caattcggcc atcctcggcg aacggctcgc gctgcgccta
     2701 gccgagcttt tcttctaccg gggtcaggtc gacagcgctg cggacgccta ccgcctgatt
     2761 gtcgaacgct acccgcgctc caccgccgtc aacgacgcct tgaaccggct ggcgcttttg
     2821 gcgttctccg gcctggcggc cagcccggaa ctctctctct tcagccgggc cgaaaaactc
     2881 cgcacccaga ggcagtttga aaaagcgatt gcgctgtacg aaaaattagc cgacgccggc
     2941 tggcagatcg accaggtgct ctgggcccag gccctggcgc tgcgtgccgc gggagagtac
     3001 ccgaaggcgc tggtggcgct ggcgaaatta aaaactgaaa ggctcaccag ccgctgggcg
     3061 gcctgcgctc tcaaattgtc gggtgaaata cgactggagg atctgggcga cccggccggc
     3121 gcgcgggccg cctttgagga aatcctgaaa aactaccccg acgccatttt caccgaggag
     3181 gtgcgcaccc ggctgaaacg cctgccgccc ggctccggcg gggccgaaag tttgcgcggt
     3241 ggttcgtaaa actaccggct gccgctggga acgaaccgct ccgagcggac atcgtgtccg
     3301 cctgaggcgg acttccgggt ggcggagatc tgcttgattt cttcttccag gcggcttacc
     3361 ttctcaatgt acccttccac ggttcgcgcc cggattttcg tggtggtccg gaccaaaaga
     3421 cccagagaaa ccagcatgaa cagcaccgaa ttcacctggc ggaagatttc gccgtagttg
     3481 aagagactgt ccatgaaggc gagcactccc agacagaaca taatggcaca ccacgctaac
     3541 atgctttcct ccttctcacg ttcgagccgg ccgtccgctg gtgccctgcc ttgacaaggt
     3601 gcaccccggg tcgtaccatt gccctgtttt tgttttcggc aaatttgacg ctcccttaag
     3661 cgacgaagag tatgccgggt gaatttcaga acgatacact gaagagattt tcgcaccttg
     3721 actggctgat cggcgctttg gttttcctgg ccagtttctt cgtcttttac ctgacgaagg
     3781 cgccgagcgt gtcgttctgg gactgcgggg agtttgtggc ctgtgcggcg atcttgggga
     3841 ttccgcaccc gccagggtcg cctttgatgg tggttttggg gcgggtgttc acgcttttgc
     3901 ctctcaatcc ggacattgcg gtgcgggtga atctcatctc ggttgtgacc t
//
LOCUS       DBNGZD010000053        10326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_285202, whole genome shotgun sequence.
ACCESSION   DBNGZD010000053 DBNGZD010000000
VERSION     DBNGZD010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10326
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_285202"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(749..1351)
                     /locus_tag="AC16GM_01485"
     CDS             complement(749..1351)
                     /locus_tag="AC16GM_01485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2922379.1"
                     /translation="MLKVLVVLTFPLLVLGSPNKEVKKPVTDKKPQAATQAKYPQVVI
                     ETDFGNITVELYPDSAPKTVENFLKLEEKKFYDGLTFHRVEPGFVIQGGDPKGDGTGN
                     AGYSIPAEFNGRKHLEGTVAMARANDPNSASCQFYICLAPQPKLDTLGYTVFGQVISG
                     MDVVKKINQVPVTWTGAFNRPVKPVYMKRVSLKKSVSPPK"
     gene            complement(1469..2029)
                     /locus_tag="AC16GM_01490"
     CDS             complement(1469..2029)
                     /locus_tag="AC16GM_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922380.1"
                     /translation="MTGLAALWLPILLSSVIVFLLSWVIHMLPLWHKNDYPKMSNEDA
                     VRDAIRPLAIPPGEYIVPRAASAKDMKNPEFIEKLKKGPVLMLTVWRNEPFSMGKSLV
                     LWFIYCVVVGIFSAYVAGRALPSGTEYLHVFRFAGVTAFTAYAVALWQNSIWYKRSCS
                     VTAKLTLDGLIYALLTAGTFGWLWPR"
     gene            complement(2206..2427)
                     /locus_tag="AC16GM_01495"
     CDS             complement(2206..2427)
                     /locus_tag="AC16GM_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922381.1"
                     /translation="MKKFMAAVFVLTVALAFLGGLLIADGQAKPPKPPPPPPPQPCDH
                     VCFEFNGCICCQDCCPGQVCTDVVCVCTA"
     gene            complement(2562..2762)
                     /locus_tag="AC16GM_01500"
     CDS             complement(2562..2762)
                     /locus_tag="AC16GM_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922382.1"
                     /translation="MKKAMAMVVVLTVVLAFLGGLFIAESQAKPPKPCQRQCVTDEFG
                     CFCCRDCCPGQICPAFVCVCPL"
     gene            complement(2965..3150)
                     /locus_tag="AC16GM_01505"
     CDS             complement(2965..3150)
                     /locus_tag="AC16GM_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922383.1"
                     /translation="MKKLMAIVVLLTVALAFCGGLFISQSQADPCKNFCTTINGCTCC
                     QHCCGKICTQLPCWCIL"
     gene            complement(3230..4519)
                     /locus_tag="AC16GM_01510"
     CDS             complement(3230..4519)
                     /locus_tag="AC16GM_01510"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR2922384.1"
                     /translation="MSPVMTAERPEKIVPKEINPFANAQMQLDSAAKILALEPTLAEA
                     LRWPERELWVRIPVRMDNGKIKSFTGFRVQYNDARGPCKGGIRFHPDETIDTVRALAA
                     WMTWKTALLDLPLGGAKGGVICNTKQMSEGELERLSRGYIDHVAPFLGPDKDIPAPDV
                     YTTPQIMAWMMDEFSILRGSYAPGVITGKPLSVGGSAGREDATARGAVIVLREAGKAF
                     EIDLKKSTLAIQGFGNAGYFLAKLAPEILGVKVVAVSDTHGGIYRLEGLAADAVEAHR
                     EKTGSVIGFPGAQEISNAKLLELPVTILCPAAIEDQITSQNASKIQASIVAELANGPT
                     TPEADEILEKNGVKVIPDFLCNAGGVTVSYFESVQNNYNYYWDEEAVHRKLDKKMTKA
                     FADTFAASQQYRVSLRMGAYCVAVSRVVEAMQARGWI"
     gene            complement(4911..6062)
                     /gene="ftsZ"
                     /locus_tag="AC16GM_01515"
     CDS             complement(4911..6062)
                     /gene="ftsZ"
                     /locus_tag="AC16GM_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR2922385.1"
                     /translation="MFDFDFEHDGFAKIKVVGVGGAGGNAVNRMIDAKLSGVDFITIN
                     TDAQVLAASKAPHRVQIGTDLTKGLGAGANPEIGRKAIEEDRDTVREMLAGADMVFVT
                     AGMGGGTGTGAAPIVAEIGRQAGALTVGIVTKPFEFEGRKRMQRAFDGLEELKCRVDT
                     LLVIPNQKLLSIVAKDVSLNDAFRIADDVLMQATRGISELITVPGLINCDFADVRTVM
                     AEMGDAIMGTGVGQGENRAHLAAQGAISSPLLENVCIAGAKGVLVNVTGGPEMTLHEV
                     NEATSIVYKAAGEEANIIFGAVVDQNLADEMRVTVIATGFGSIRSDYAHPAMLREVDL
                     FRPVRRAAAAKQETANGYEKAEPVPVEVCPKIDVAELEVPTFLRRKLDR"
     gene            complement(6081..7316)
                     /gene="ftsA"
                     /locus_tag="AC16GM_01520"
     CDS             complement(6081..7316)
                     /gene="ftsA"
                     /locus_tag="AC16GM_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR2922386.1"
                     /translation="MVGAETVAALDLGTTKVSVLVAEVERDLWPKLLGIGVVSAAGIR
                     QGQVVDLPGATHSIRQAVQDAELMAGIKITRVVVGISGEQIQNFHSRSVVSVGTPRLR
                     QEVSPADVGRVLAAARLLEFPSDRQVLHVLPTRFAIDDQDGIRQPAGMVGVRLSADVQ
                     VVTCGRTATENLLRAVGKTELEVSELVAEPLAASRAVLTPEEKETGVALIDLGGSTTQ
                     LAVFLEDALALTACIPLGGRNATNDLAVGLRLPGDKAEELKLIHGAAYAPAVDPDEVV
                     LIPATGSRPPKQISRTVLASIIQPRLEEIFGLLARQIRRKSYAEPVPASVVLTGGGAL
                     LAGVCHLAEHCFDLPVRIGIPQGFTGLVERAAHPRHATGVGLVLYGAASPRQNGHRPP
                     HWERTVRKLTRVWREYFQP"
     gene            complement(7303..8064)
                     /locus_tag="AC16GM_01525"
     CDS             complement(7303..8064)
                     /locus_tag="AC16GM_01525"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR2922387.1"
                     /translation="MTPKREIFFRVLIVFGLLLAVGTATAERAGLFRLRRVMVSGNTV
                     VSREEILQRAKLPFGANLFRVDIEKIARRVAADQRFWGVTVSRQLPDAVEVSVTEKKP
                     ALTLNTGQLWGLTANLEVLPEEEASLSLPVVTAGYWQPDRFYQSNRQNGLGLAAEAYR
                     ALVACDSRLLEAISEISVAPSGDVVLYLVPKGAAVNFGTGDFSIKVKRLAALLAEFGG
                     ILPDLAYDLRFAGNLFVTPKDSLSVAKARSRRGRG"
     gene            complement(8061..9443)
                     /gene="murC"
                     /locus_tag="AC16GM_01530"
     CDS             complement(8061..9443)
                     /gene="murC"
                     /locus_tag="AC16GM_01530"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR2922388.1"
                     /translation="MSLRKIKRLHFVGIGGTGMCGIAEVLLSSGYQITGSDLKFSEAT
                     DRLVKLGAVVYAGHRAEQVRDAQVVVISSAVQPDNPEVQYALSHGIPVIRRAEMLGEL
                     MRMKFGIAVAGTHGKTTTTSMIGEILTTAGLDPTVVVGGRVVNLQTHAQIGGSEYLVA
                     EADEFDRSFLRLTPQVAVLTNIEADHLDCYKDIEDIKATFIQFANQVPFYGTVVLCAD
                     DANVAIIRPQISRPVVTYGLSGAVDFSAVDLAFSERRSAFNVFRGVEHLGQVALAIPG
                     VHNVKNALAAIAVGFEVDIPWEKIKTGLEAFRGVHRRFEVVGDVNGILVVDDYAHHPT
                     EIAATLAAARQGYPQRRRVAVFQPHLFTRTRDFFREFGEALRGADVAIVTDIYPSREK
                     PLPGITGQMVADAAVKAGHKQIHYVPDKNRVTGFLGSRLLPGDLVITLGAGDIYRVGL
                     ELVELLKTTV"
     gene            complement(9511..>10326)
                     /locus_tag="AC16GM_01535"
     CDS             complement(9511..>10326)
                     /locus_tag="AC16GM_01535"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR2922389.1"
                     /translation="VTRRLASKVNAVCLAYAEAAAYLKRQDNITVTGNPVRPDIASGK
                     REEAVKVFGLDPAKKTVLVIGGSQGAASINRALLGSFDQFEAESPVQLIWQTGRGMFE
                     EVSRTVAGRKVIAGIFPFIDQMPLAYAAADLVVCRSGALTLAELAVCGKPAILIPYPH
                     AAADHQRHNAQVVQKEGAAEMILDFELEKISLGERIMSLLHDGGRLKRMAEKSRALAR
                     PDAATTIVGLMDRLLAKAPQANPVARTRTEMQNLPSIALESPALKLGITLFRR"
BASE COUNT         2147 a         3208 c         2828 g         2143 t
ORIGIN      
        1 gcccgatggt cctatacttt cggttttaca tgaccgctgt tcatttcacc tgttggctaa
       61 catactttaa tggcaggcat cgtgaggaat aatcaccgca ccaccgcact tacagcaaac
      121 cagtgcggct ctgataacta cccccaacct atggccttaa aagaaattac tgggtagtct
      181 gctggctaga gtggcagcag gtcgcatcac agattgatgg acaatcaacc gtccaacaat
      241 cgaaacccgc tgaccagcca gaaacgtcag tgcaattggt gctacagtgg cagcagaaat
      301 aatagccttg tcgagcctca tcgggtgtac aagttgctaa ccaaccataa gcgggacacc
      361 catagtaatg aggatctaaa gaaacagcct taatggtact cagtgtcact aagaacgaaa
      421 ctcctatagt ggcaactact aatgctgcca tttggaatag ccgtcgcctc ttccgcataa
      481 tacctccacc tctaggctaa cttttactca atacttaaca ccattcaatt gcctaattta
      541 aatatatacg aagaacctgt caagactttt ttcacttttt caccggcatg gggatttcta
      601 aggtgcttga cattagttaa aatatttcat tatcaggaaa ttctcgaggc tcgaaaaact
      661 tagatgttgc acaaaatgac gctaagtcct cgaagggttg ttcctgttca aacggcatct
      721 cgaactacct attgagaaga agattatctt actttggagg tgaaactgac tttttcaagg
      781 acactctctt catgtagacc ggcttgacgg gtcggttgaa tgctccggtc cacgtcacgg
      841 gcacttggtt gatctttttc accacgtcca taccggaaat aacctgaccg aagacggtat
      901 accccaacgt gtccagcttc ggttgtggtg ccaaacaaat gtagaactgg caagaggccg
      961 aattggggtc gttcgcacgc gccattgcca ccgtgccttc gaggtgtttg cggccgttga
     1021 actcggcggg gatagaatat cctgcgttgc ccgtaccgtc gccctttgga tcgccaccct
     1081 gaatgacaaa tcccggttcg accctgtgga aggttaaacc atcgtaaaac ttcttctcct
     1141 ccaatttcag gaaattctcg acggtcttcg gcgccgagtc cgggtaaagt tcaacggtaa
     1201 tgttcccgaa atcggtttcg atgacaacct gcgggtactt cgcttgggta gcggcctgcg
     1261 gctttttatc agtcaccggc ttcttcacct ctttgttggg gcttcccaga acgagtaacg
     1321 gaaacgtcag taccaccaga acttttagca tgcacctctc ctttttagtt catttttcat
     1381 aacaccgcgt caagatccga gcgccgctga agcttgccac gcggtgctcg tcagcttcta
     1441 agcgttgttg ggccgcgcaa accctgacct atcgcggcca gagccatccg aacgtacccg
     1501 ccgtcaagag cgcgtagatc aagccgtcca gggtgagttt ggccgttacg ctgcaggaac
     1561 gcttgtacca gatggagttc tgccagagcg ccaccgcgta ggccgtaaac gccgtgaccc
     1621 cggcaaagcg gaagacgtgc agatactccg tgcccgaggg cagcgcccgc ccggccacgt
     1681 aggcggaaaa aatcccgacc accacgcagt aaataaacca gaggaccaag cttttcccca
     1741 tcgaaaaggg ctcgttgcgc cagaccgtaa gcatcagaac cgggcctttt ttcagcttct
     1801 cgatgaactc cgggtttttc atatccttgg cggaggcggc gcggggaacg atatattccc
     1861 ccggggggat ggccagcggg cggatggcat ccctgaccgc atcctcgttc gacattttgg
     1921 ggtaatcgtt tttatgccag agcggcagca tatggatgac ccagctgagc agaaagacga
     1981 tcaccgacga caaaagaatt ggaagccaca acgcggccaa cccggtcata acgacctcct
     2041 ttgctgagac cactcgtttc ctacgggcag actcctccgt ccttcgtcca tgcgcggcct
     2101 aacataacga ataccgaaca gttgcgcaaa agttttcccg aacaaaaaaa gcaaaccctg
     2161 ggccagataa tcaccagggt tcgccctcgt ccgtttcccg gcaggttacg ccgtgcagac
     2221 gcagaccacg tcggtgcaga cctggcccgg acagcagtcc tgacaacaaa tgcagccgtt
     2281 gaactcgaag cagacatgat cacagggctg cggcgggggt ggcggcggcg gtttcggcgg
     2341 tttggcctga ccatcagcaa tcagaagccc gccgaggaac gccagcgcca cggtcagtac
     2401 gaacacggcg gccatgaact tcttcacgtg cactcacctc ctctcttgtt gtgagcgatt
     2461 cctcaagtga acgcctttcc cggttcctcc gctgggaatc ataaacggca gctccccggg
     2521 atggctgccg cctgggtccg ctttcgtccg cgtctgactt tttacagcgg acaaacgcag
     2581 acgaacgccg ggcagatctg accggggcag cagtcccggc aacagaagca gccgaattca
     2641 tccgttacgc actgccgctg acaaggtttc ggcggcttgg cttggctctc agcgatgaac
     2701 agccccccga ggaatgcgag caccacggtc agcactacga ccatggccat ggctttcttc
     2761 atgcgaattc acctccttcc aatggctgaa cgccccaaaa aaggccttct ggacaaaacg
     2821 attccctgta cccgacttct ccccgcaatg ccttcggctc aacgcttttg ctgcagaagt
     2881 cgcaccgatc cggttcatcg gcttccatgt ctgcaagaat gcgaaccctg gagcaaccgg
     2941 cctccagggt tcgctcgcgc ccgcttacag gatgcaccag caggggagct gggtacagat
     3001 ctttccgcag cagtgctggc agcaggtaca gccattaatc gtcgtgcaga agtttttaca
     3061 cggatcggcc tggctctggc tgatgaagag ccccccgcag aacgccagcg ccactgtgag
     3121 cagcacgacg atggccatga gtttcttcac actacctcac ctccttccgt ggataatcgc
     3181 cggataaact ctcggagttc cccaaatccc aattcctact atccggtctt tagatccacc
     3241 ctcttgcctg catcgcctca accacccggc tcaccgccac acagtacgca cccatccgca
     3301 gcgacacccg gtactgctgg ctcgcggcaa aagtatccgc aaaggccttg gtcatttttt
     3361 tgtctaattt gcggtggacg gcctcttcgt cccagtagta gttgtagttg ttctgcaccg
     3421 attcgaagta ggagacggtc actccgccgg cgttgcagag gaagtcgggg atgaccttca
     3481 cgccgttttt ctctaagatt tcatccgctt ccggcgtggt ggggccgttg gccagctccg
     3541 ccacaatcga ggcttgaatt ttgcttgcgt tctggctcgt gatctgatcc tcaatcgccg
     3601 ccggacagag aatcgtcacc ggtaattcca gaagcttcgc attcgagatt tcctgagcac
     3661 ccggaaaacc aatgaccgag ccggttttct cccggtgcgc ctccactgcg tcggccgcaa
     3721 gtccctcgag ccggtaaata ccgccatgcg tgtcggagac ggcaacgact ttaactccca
     3781 gaatctccgg cgccaatttt gctaaaaagt agcccgcatt accaaaaccc tggatcgcca
     3841 gagtcgattt tttcaagtcg atttcgaacg ccttccccgc ctcacgcagg acgatcaccg
     3901 ccccgcgcgc cgtggcatct tcgcgtcccg ctgacccgcc caccgacagc ggtttgccgg
     3961 taatcacccc gggagcgtaa ctgccgcgca atatggagaa ttcatccatc atccaggcca
     4021 taatctgcgg agtagtgtag acatcgggcg cagggatgtc tttgtccggt cccagaaaag
     4081 gagccacatg atcaatgtag ccccgggaaa gccgttccaa ctcaccctcg ctcatttgct
     4141 tcgtattgca aatcaccccg cctttcgccc cgcctaatgg caaatcaagt aaagcggttt
     4201 tccaggtcat ccaggcggcc agggcccgca ccgtgtcaat ggtttcgtcc ggatggaagc
     4261 ggataccccc cttgcacggc ccgcgggcgt cgttgtactg cacccggaag ccggtgaagg
     4321 acttgatctt gccgttgtcc attcgcactg gaatacggac ccagagctct cgctccggcc
     4381 agcggagtgc ctcagccagc gtcggttcta acgctaaaat ctttgcggct gaatcgagtt
     4441 gcatttgggc attggcaaaa ggattgatct cttttgggac aattttctct ggacgttcgg
     4501 cggtcattac tggtgacatt attcctcccc gggttacatg tctcatgcgt agctttgaag
     4561 agtcaaagac ctggtcgatt ttcagaccca cttctatccg tacggaccga actttttatg
     4621 gacgttggcc gcttctgact tgggacgaat accgccacca gcgcgatttt caggcggtct
     4681 gcagagaaag gtttcattcg aactccgata attacgtctt aaaataggcg gcgagagaca
     4741 ggatgtcaac cgtttttttg atggaaaagg attcaagcca aactctgtcg ggtatggggg
     4801 tgggggctca ggagggccga atccactccc gccacgcaaa aacccccggg aaaaggctca
     4861 tcgggggctc ttgttttaag cggtacgatc cgctgtctgt ttgctgtacg ttagcggtcc
     4921 agcttccgcc gcaggaaggt cggcacttcc aactcggcca cgtcgatttt cgggcacact
     4981 tccacgggaa ccggctcggc cttctcgtag ccgttggccg tctcctgttt ggcggccgcc
     5041 gcgcgccgca ccggacggaa gaggtcaact tcccgcagca tcgccgggtg ggcatagtcc
     5101 gagcgaatgc ttccgaagcc ggtggcgatc accgtcaccc gcatttcatc cgcgagattc
     5161 tgatccacca ccgcgccgaa gatgatgttc gcctcttccc ccgccgcctt gtagacgatg
     5221 gaagtggcct cgttcacctc gtgcagggtc atttccggcc cgccggtgac gtttaccagc
     5281 acacctttgg ccccggcgat gcagacgttt tccaaaagcg gactggagat cgccccttgc
     5341 gccgccagat gggctctgtt ctctccctgg ccgacgcccg tgcccatgat ggcgtcgccc
     5401 atttcggcca tcaccgtgcg cacgtcggcg aaatcgcagt tgatcagccc cggcacggta
     5461 atgagttcgg aaatcccccg cgtcgcttgc atcaaaacat cgtcggcgat ccggaaggcg
     5521 tcgttgagcg aaacgtcttt ggccacgatg gagaggagtt tctgattggg gatgaccagg
     5581 agcgtatcga cccggcactt cagttcctcc aacccgtcaa aggcgcgctg catccgtttg
     5641 cgcccttcga attcgaacgg tttggtgacg atgccgaccg tgagggctcc ggcctggcgg
     5701 ccaatttcgg ccacgatcgg cgccgcgccg gtgccggtgc cgccgcccat cccggcggtg
     5761 acgaagacca tgtcggcgcc ggccaacatt tcgcgcaccg tgtcgcggtc ttcttcaatc
     5821 gccttgcgcc cgatctccgg attggccccg gcgcccagcc ccttggtcag gtcggtgccg
     5881 atctgtaccc ggtggggtgc cttggaggct gccaaaacct gcgcgtcggt gttgatggtg
     5941 atgaaatcga cgccggagag tttggcatct atcatccggt tcaccgcatt cccgccggcg
     6001 ccgcccaccc ccaccacttt gattttggcg aaaccgtcgt gctcgaagtc gaagtcaaac
     6061 agcatcgaac ccctcccggg ttaaggttga aaatactcgc gccacactct cgtcaatttc
     6121 cttacggtgc gctcccagtg cggcggccgg tgcccgttct gccgcggcga ggcggccccg
     6181 tacaaaacca ggcccacgcc ggtggcgtgc ctcggatgcg ccgcgcgctc caccaggccg
     6241 gtgaagccct gcggaatgcc gatccgcacc ggtaaatcga aacagtgttc ggccaaatga
     6301 cagaccccgg ccagaagcgc accgccgccg gtgaggacga ccgaggccgg caccggctcg
     6361 gcgtacgatt tgcgccggat ctgccgggct aaaagcccga aaatctcttc taagcgcggc
     6421 tgaatgatac tggccagaac cgtccgcgaa atttgcttgg gcggacggct gcccgtggcc
     6481 gggatcaaaa ccacttcatc aggatcaacg gcgggggcat acgccgcacc gtgaatcagc
     6541 ttcaactctt cggctttgtc gccgggcaag cgcaagccca ccgccaggtc gttggtggca
     6601 ttccgcccgc ccaggggaat acaggcggtc aaggccaggg catcctccaa gaaaacggct
     6661 aactgcgtgg tgctgccgcc caggtcgatc aaagcgaccc ccgtttcctt ttcctccggc
     6721 gtcaaaacgg cccggctggc agccaaaggt tcagccacca gttcgctcac ctcgagttcg
     6781 gttttcccta cggcccgcag caaattttcg gttgccgtcc ggccgcaggt gacgacctgc
     6841 acgtcggcgg aaaggcgcac gccgaccatg ccggccggct ggcggatccc gtcctggtcg
     6901 tcgatggcaa agcgggtcgg caagacgtgc agcacctgac ggtcggaggg aaattccaaa
     6961 agccgggccg ccgccaggac ccgcccgaca tcggcggggg aaacctcctg gcgtaaacgc
     7021 ggggttccca ccgacaccac gctccggctg tgaaagtttt gtatctgctc gccggaaatt
     7081 ccgactacca cacgggtgat tttgattccc gccatgagtt cggcgtcctg gaccgcctgg
     7141 cgaatcgaat gggttgcccc gggaaggtct accacctgtc cttgccggat tcccgcggcg
     7201 gaaaccacgc cgatacccag gagtttcggc cagagatcgc gttccacttc ggccacgagc
     7261 acgctcacct tggtggtgcc caaatctaag gcggcgaccg tttcagcccc gaccacggcg
     7321 gctcctcgct ttcgcaacgg aaagtgaatc tttcggcgtg acgaaaagat tgccggcaaa
     7381 cctgaggtcg taggccagat cgggcagaat gccgccgaac tcagccaaaa gcgccgccaa
     7441 ccgcttgact tttatcgaaa agtcgccggt gccgaagttg accgccgcac ccttcgggac
     7501 gaggtagagc accacatctc ccgacggcgc tacgctaatt tccgagattg cttccaagag
     7561 acgggaatcg caggcgacca gggcccggta cgcctctgcc gccagaccca acccgttctg
     7621 ccggttcgat tggtagaaac ggtcgggctg ccagtatccg gcggtcacga ccggcaggga
     7681 aagggaggct tcctcttccg gaagcacttc taagttggcc gtgagacccc agagttgccc
     7741 ggtgttcagg gtcagagccg gttttttttc ggtcacggaa acctcgaccg cgtcgggcaa
     7801 ttgtcgcgaa accgttacgc cccaaaacct ctgatctgcc gccacccggc gcgcgatttt
     7861 ttcgatgtcg acgcgaaaca gattcgcccc gaacggcagt ttcgctctct ggagaatttc
     7921 ttcccggctg accaccgtat taccgctgac catgactctg cggagccgaa acaacccggc
     7981 ccgctcggcg gtggcggtcc ccacagccag gagaagccca aacacgatga gaacgcgaaa
     8041 gaatatttct cgtttcggcg tcatacggtc gtcttaagca gctccactaa ttccagtcct
     8101 acccggtaaa tgtccccggc acccagggtt atgacgaggt cgccgggcaa aagccgcgat
     8161 cccagaaagc cggtgacgcg atttttgtcc ggaacgtagt gaatctgttt gtgcccggct
     8221 ttgaccgccg catctgccac catttggccc gtaatgcccg gcaaaggctt ctcgcgggag
     8281 gggtaaatgt ctgtgacaat agcgacgtca gcaccgcgga gtgcctcacc gaattcccgg
     8341 aaaaagtccc gcgtgcgggt gaaaaggtgc ggctggaaga ccgccacccg gcgcctttgg
     8401 ggatacccct gacgtgcggc tgcgagcgtg gcggcaatct cggtcgggtg gtgggcgtaa
     8461 tcgtcaacga ccagaatgcc gttaacatca cccacgacct caaaccgccg gtgcaccccg
     8521 cggaacgctt ccaggccggt cttgatcttt tcccacggga tgtcaacttc aaagcccacc
     8581 gcaatcgccg ccagtgcatt tttgacgtta tgtactcccg gaatggccag cgcaacctgg
     8641 cccagatgct cgacgccccg gaaaacattg aacgcagaac gccgctcact aaaagcgaga
     8701 tcgacagccg aaaaatcaac cgccccggaa agtccgtagg tcaccaccgg ccgggaaatc
     8761 tgcgggcgaa taatagcgac gttcgcatcg tcggcacaga ggactacggt gccgtaaaag
     8821 ggaacctgat tggcgaactg gatgaaagtc gccttgatgt cttcgatgtc cttgtagcaa
     8881 tcgaggtgat cggcttcgat attggtcagg accgccacct gcggcgtgag ccgcaaaaac
     8941 gaacggtcaa actcgtcggc ttcggctacc aaatactcgc tcccgccgat ctgcgcatgg
     9001 gtctggagat tcacgacccg gccgcccacc accacggtcg gatccagccc cgcggtggtc
     9061 aggatttcgc cgatcatcga tgtcgtggtg gtcttcccgt gtgtcccggc cacggcaatg
     9121 ccgaatttca tccgcatcag ctcgcccagc atctccgccc gccggattac cggaatgccg
     9181 tgggaaaggg cgtactggac ttcgggattg tccggttgca ccgccgagga aatgaccacc
     9241 acctgcgcat cccgcacctg ctccgcccgg tggcctgcgt agaccaccgc ccccagtttg
     9301 accagccggt cggtcgcctc cgaaaacttg aggtcggagc cggtgatctg gtagccggag
     9361 gagaggagca cctcggcgat gccgcacatc ccggtgccgc cgatgccgac gaagtgcagg
     9421 cgcttgatct tccgcaggct cataccgatt cgtttgggtg tgacactctt tttcctgacg
     9481 accgtctcga tagtatccat aggatggggt tcatctccgg aagagggtga taccgagttt
     9541 caatgcgggc gactcgagcg cgatagatgg gagattctgc atttcggtac gggtgcgggc
     9601 caccgggttc gcctgcggtg ccttggccag caaccggtcc atcaaaccga caatggttgt
     9661 cgccgcatcg gggcgggcca gcgcccggct tttctcggcc atgcgcttca gtcggccgcc
     9721 gtcatgcagg aggctcatga ttcgctcgcc cagactgatc ttctctaatt caaaatccaa
     9781 aatcatttcc gccgcaccct ctttttgcac tacttgcgcg ttgtggcgct ggtggtcggc
     9841 ggcggcgtgg gggtagggaa tgagaatggc gggcttgcca cagaccgcca gttcggccag
     9901 agttagcgct cccgaccggc agacgaccag gtcggctgcg gcataggcca gaggcatttg
     9961 atcgatgaaa gggaaaatcc cggcaatcac cttccgaccc gctaccgtgc gacttacttc
    10021 ttcgaacatg ccccgcccgg tttgccagat aagctgaacg ggactttccg cttcaaattg
    10081 atcgaaagat cccaagagcg cccgattgat ggaggccgct ccctggctgc cgccgatcac
    10141 cagcaccgtt ttcttcgccg gatccagtcc aaaaactttg accgcttctt cccttttacc
    10201 cgacgcaata tccggccgca ccggattgcc ggtgacggtg atgttgtcct gccgtttgag
    10261 ataggccgcc gcctcggcgt aggccaggca gactgcgttg accttcgacg ccaagcgccg
    10321 ggtcac
//
LOCUS       DBNGZD010000054         6019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_840814, whole genome shotgun sequence.
ACCESSION   DBNGZD010000054 DBNGZD010000000
VERSION     DBNGZD010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_840814"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2386
                     /gene="leuS"
                     /locus_tag="AC16GM_01540"
     CDS             <1..2386
                     /gene="leuS"
                     /locus_tag="AC16GM_01540"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR2922390.1"
                     /translation="TVIPMFAYPSGDIHMGHFRNYTITDAIARFKRMQGYDVLHPFGW
                     DAFGLPAENAAIKRGIHPREWTLNNVAVSKATLKRVGILFDWEREVTTCLPDYYKWTQ
                     WLFLKLWEKGLAYRAEAPVNWCPSCQTVLANEQVEQGRCWKCDSVVVKKELTQWFFKI
                     TAYAERLLKDIEALTGWPENVKTLQKNWIGKSTGCFIDFPLEGSDLKLPVFTTRPDTV
                     YGVTFLSIAPDSRLFKKIEIAGPRKKEVAAYVEKAGARTEIERAAASEEKDGVFTGRF
                     VRNPLSGEQVPLWVADYVLAGYGSGVVMGVPAHDQRDFEFARKYRLPVKVVIVPPEAG
                     AGEKLPEAERMEAAFTDYGRMTNSGPFDGKVGREAIKAVIDHVEKQGWGKGAVQYKLR
                     DWLISRQRYWGCPIPIIHCPDHGEVAVPEKDLPVLLPPEVKDYIPKGRSPLADLPEFV
                     NTVCPKCGKPAQRDVDTMDTFIDSSWYFLRYADPHNTREPFDRKKANTWLPIDLYVGG
                     IEHATGHLIYFRFFTKVLYDLGYLGFEEPAARLFNHGMVLDAGGQVMSKSLGNVVTPD
                     EVMTKEGADVSRLTMYFAAPSNREMFWSTEGVVGISRFLTRLWKLIEGRGKIDSAAVF
                     QLVAASQINGADLKLYRKLHQTIKKVTEDFETLQFNTAIAALMELLNEIPAQKPERSR
                     VYDLTLIRIAQLLAPLAPHIAEEFWQILGFSGSIFRSQWPEHDPAALVEEELTIAVQV
                     NGRLRATVNVPVTADEGFITGQAQQHDRVKPHLKDKKVLKTVYVPRKLVNIVVQ"
     gene            2773..3363
                     /locus_tag="AC16GM_01545"
     CDS             2773..3363
                     /locus_tag="AC16GM_01545"
                     /inference="COORDINATES: protein motif:HMM:NF037752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HIR2922391.1"
                     /translation="MLRGATVLAVVMLAVIVYPSVGNSFVYKVPFTITAGEIQANGGN
                     PFPAVTIDQVDLGGLQGLSAGPCQANALISGIKITRRGNAKPLLSNVQPPGYAVAVTI
                     SRDLGLGEVALPAGEYDAWFKSDKGPCDTPKARITKKGSGAFSISTVAAVTTIDTATV
                     EPVQLPTLNQWGLILLVAVLGGIGTFVIVRRRWTAV"
     gene            3471..>6019
                     /locus_tag="AC16GM_01550"
     CDS             3471..>6019
                     /locus_tag="AC16GM_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922392.1"
                     /translation="MLVSTAGTVSLAKSPRALPDKVDGAALSSFLRAPTSEILQQSSV
                     AAVSDTCGDTCDVGYDDGVLTTKATAAFGSPGDRLAVGIVRPAKFSKLIAAKYFLIWA
                     NLVPPKPNGPFRVRVFRDSTGIPSRADLIPGTLTYLAPSQGWNSVPLASYNIIPPDDT
                     TYVTLEYLTGPPVANQTPDVMVDAAVNQDGGGLMYSVGVGNWQSVQTTYGPSAGDLFI
                     RGVFELPRRGDYGDAPDSSNGNIAAAGSGFPTQYFTKNATVIGRRGPFHRDTTQFWLG
                     DSGNAPTRERNPQNNTGDGDRDSSKINGQFYTAVVGSDTLGWFIVPVSVAGSASAAVR
                     VLNILYDQNKNGKWQNTSAAFPEWLVRNAQVFTNPGMTENYVLGPFRLKNVPPGPIWS
                     RFTFTSDSIPQALFGTGWDGSGPDTGFAAGETEDFNLLGSVSAESAALVQVAAPVTFD
                     AFEADTGRSTREVRFINYGGKALTASEIRFFALGDPDTNFILTLVDSAGQNSTNFTIP
                     ANSTVTFRYRAQFNRSSDDTVHRADRTRAFAWQLRGNSITTPLLSQAFTTFVEHVRPQ
                     FVQPVQNSFTRSACDFSFDFLVTAMDIDEEILFPLNIRYCGTCFRETVSLRAPVNLPT
                     FFPDTVAALGTNNPGRFRWTPGVADTGVWLAKFVARDSVDKMEDTTDIFISVSATSAT
                     GVIAVRHIVVTVPESTTCFSVAVRVFNADFLRTIKIPLVYDSLICLKAITYDSTRLAN
                     SNILNQRFLDSTSYRNHNGLDTLVLTFFRTVGFSDLEPGCGTIANIWFSTKVTDTTVN
                     PLDTVFINSSERPFFETAGGQEVLNLQFQNEPLHPQVLLGDLDCDTVRDIN"
BASE COUNT         1382 a         1613 c         1648 g         1376 t
ORIGIN      
        1 caccgtcatt ccgatgttcg cctatccctc gggcgacatc cacatgggtc acttccgcaa
       61 ctacactatc acggacgcaa tcgcgcggtt caagcggatg caggggtacg acgtcctcca
      121 tccattcggc tgggatgcct tcggcttgcc ggcggaaaat gccgccatca agagaggcat
      181 acatccgcga gaatggaccc tgaataatgt tgccgtgtcg aaagcgacgc tcaaacgggt
      241 ggggatttta ttcgactggg aacgggaagt caccacctgt ctgcccgact actacaagtg
      301 gactcagtgg ctctttttga aactgtggga aaaaggcctg gcctaccgcg ccgaagcgcc
      361 ggtgaactgg tgcccttcct gccagaccgt tctggccaac gaacaggtgg aacagggacg
      421 ctgctggaag tgcgattcgg tggtggtcaa aaaagagctg acgcagtggt tcttcaaaat
      481 cacggcctac gccgagcgcc tgctcaagga catcgaggcc ctgaccggct ggccggaaaa
      541 tgtgaagacg ttacaaaaga actggatcgg caaaagtaca gggtgcttca tcgactttcc
      601 gttggagggt tctgacctga aactcccggt cttcaccacc cggcccgaca ccgtctacgg
      661 cgtcaccttt ttgtccattg cgccggacag ccggctgttc aaaaaaatcg agatcgccgg
      721 cccccggaaa aaagaagtgg ccgcgtacgt cgaaaaggcc ggagcccgca ccgaaatcga
      781 gcgggcggcc gccagcgaag agaaagatgg cgtatttacg ggccgcttcg tccgtaatcc
      841 gctttcgggt gagcaggtgc cgctctgggt cgccgactac gtcctggccg gttacggttc
      901 cggcgtggtc atgggtgttc cggcgcacga tcagcgcgac ttcgagttcg cccggaaata
      961 ccgcttgccg gtgaaagtcg tcattgtccc gccggaggcg ggagcagggg agaagctgcc
     1021 ggaagccgag cgaatggaag ccgccttcac cgactacggc cggatgacaa actccgggcc
     1081 gttcgacggc aaagtggggc gcgaggcgat aaaagccgtt atcgaccacg tcgaaaaaca
     1141 agggtggggg aaaggggccg tccagtataa actgcgcgac tggcttatct cccggcaacg
     1201 ctactggggc tgtccgattc cgatcatcca ttgtccggat cacggcgaag tagccgtgcc
     1261 ggaaaaggac ctccctgttc ttctgccgcc cgaggtgaaa gattatatac ccaaaggccg
     1321 ctcgcctctg gccgatctgc cggaattcgt caacaccgtc tgtcccaagt gcggcaaacc
     1381 ggcccagcgc gacgtggata cgatggatac gtttatcgac tcctcctggt actttctccg
     1441 ctatgccgac cctcacaata ccagggagcc tttcgatcgg aagaaggcca atacctggct
     1501 gcccatcgac ctctacgtcg gcggtattga gcacgctacc ggccacctga tttactttcg
     1561 gtttttcacc aaggtgctct acgatctggg ctacctgggt tttgaggaac cagccgcccg
     1621 cctctttaat cacggcatgg tcttggatgc cggaggacag gtaatgtcca aatctctggg
     1681 gaacgtggtt accccggacg aagtcatgac taaagaagga gcggatgtca gccggctcac
     1741 catgtatttc gcggcgccct ccaatcgaga aatgttctgg tctaccgaag gggtggtggg
     1801 aatcagccgc tttttgaccc gtttgtggaa actgatcgaa gggcgcggca agatcgattc
     1861 tgcggcagtc ttccaactcg ttgcggcgtc gcaaattaac ggtgcagact taaagttata
     1921 tcgaaaactg caccagacga tcaaaaaagt taccgaggat tttgagacat tgcagttcaa
     1981 cacggccatc gccgctttga tggagctttt aaacgaaatt cctgcgcaaa aaccagagcg
     2041 gagccgggta tacgacctga ctcttatccg gattgcccag ctcttggccc ctctggcgcc
     2101 ccacattgcc gaggaattct ggcaaatact cggtttttcc ggaagtattt tccgttcgca
     2161 atggcccgaa cacgacccgg cggctttagt cgaggaggaa ctgaccattg ccgtccaggt
     2221 caatggccgg cttcgggcga cggtgaacgt gccggtaacg gctgacgagg ggttcataac
     2281 cggccaggct cagcaacatg accgggtaaa accgcatctg aaagacaaga aagtgcttaa
     2341 aacggtctac gtgccccgga aattggtcaa tattgtggtt caatagaacc taaaggcgtt
     2401 cagtaagtta ggtacaaggg gaagatgggg gcctcggcgc aaaatcggtt ctttacataa
     2461 tatatcttat aggacataaa gactttgggc caattcatga tgatttccat ccgatacttt
     2521 gcccctactg acaaaatttt gcctgtaaaa gtaatttttt gcttgacaag ccaagattcg
     2581 caaaagtata ttctcccctg ccatttggtg gggccggtgc tggttgcgtg cacaaccctg
     2641 aggggggttg cttagcgaaa cagcggaggt tccttttgga agcgggaggt tgagatatga
     2701 gacagaggtt tgagctcaac cagtagcgac agagcgaatc aattcaaaat ctaaacaaga
     2761 gaggagaaag ttatgctcag aggagcgacg gtcttagccg tagtgatgtt ggctgtgata
     2821 gtgtacccga gtgtgggaaa ttcctttgtc tacaaagtcc cttttaccat tacagcgggg
     2881 gagatccaag ccaacggtgg taatccattt cccgccgtta caattgatca ggtggacttg
     2941 ggtggattgc aagggcttag tgccggaccc tgtcaggcca acgcactcat cagtggcatc
     3001 aaaattacta gaagaggcaa tgctaagccc cttctttcca atgtccaacc tcctggctac
     3061 gcggtggccg tgacaatctc tagagatttg ggattgggtg aagttgccct tccagcaggt
     3121 gagtacgacg cgtggttcaa aagcgacaaa ggaccgtgcg acaccccgaa agcacgaatt
     3181 accaaaaaag ggagtggcgc tttcagtatt tctacggtcg ccgcggtaac aacgattgac
     3241 acggcaacgg ttgagcctgt tcagttgccg acgctaaatc agtggggctt gattctcctt
     3301 gtcgctgttc tgggtggaat tgggactttc gtcatagttc gtcgtcgttg gacagcagta
     3361 taaggactgt gaggaggttg accaaggaat aggcaaagac ggggctcgca agaaagggag
     3421 agagatctat gcgtcgagga gagaagctgt acgggtgcct ttgtgcggtt atgttagtta
     3481 gtacagcagg cactgttagc ctcgctaagt cgccacgtgc actaccagac aaagttgatg
     3541 gtgccgccct ctcttctttt ctgcgagccc ctacttctga aatactccaa cagtcttctg
     3601 ttgcggctgt cagcgacacc tgtggtgaca cctgtgatgt cggatatgat gatggcgtct
     3661 tgacaaccaa agccactgca gcatttggat ctccgggaga tcgtctggca gtgggcattg
     3721 tacgacccgc aaaattctca aaactgatag cagcgaaata cttcctgata tgggcaaatc
     3781 tcgtgcctcc caagccaaac ggtccttttc gcgtacgagt gtttagagat agcacgggca
     3841 ttcccagtag ggctgattta attccaggca ctctcacgta tctcgctccg agtcaaggtt
     3901 ggaattccgt gccccttgct tcttacaaca tcatcccccc tgatgacaca acgtatgtga
     3961 cactcgagta cctcacgggt cccccggttg ccaatcagac tcccgatgtc atggtggatg
     4021 cggccgtaaa tcaagatggc ggcgggttaa tgtattctgt aggggtcggt aattggcagt
     4081 cagtacagac tacctacgga ccaagcgctg gcgatttgtt catacgcggt gtttttgaat
     4141 tgccccgacg tggtgattac ggtgacgctc cggattcatc gaacggtaac atcgccgcgg
     4201 cgggctccgg cttcccaacg cagtacttca ccaagaacgc taccgttatc ggcaggcggg
     4261 gtcctttcca tcgggataca acccaattct ggcttggcga ttccggtaat gccccgaccc
     4321 gtgaacgcaa ccctcagaac aacacggggg acggtgatcg cgatagttca aagattaacg
     4381 ggcaattcta caccgctgtc gtgggcagcg atacgctcgg ctggttcatc gtcccggttt
     4441 ctgtggccgg gtcagcgagc gctgccgtca gggtccttaa catcctttac gaccaaaaca
     4501 agaatggtaa gtggcagaat accagtgcgg cctttccaga gtggctcgtc cgcaacgcac
     4561 aggttttcac caacccggga atgacggaaa actacgtcct cggccccttc cggctcaaga
     4621 atgtgccgcc cggacctatc tggagccgct ttaccttcac cagcgattct attcctcagg
     4681 cactgtttgg gaccggctgg gacggcagcg gcccggatac cggttttgcg gccggtgaga
     4741 cggaagactt taatttactg ggaagcgttt cggctgaatc ggcggcgctg gtccaggtgg
     4801 ccgcaccagt taccttcgac gcgtttgaag cggacaccgg ccggagcaca cgagaggtcc
     4861 ggttcatcaa ttacggaggg aaagccctga ccgcctcgga aattcgtttc tttgcgctcg
     4921 gagaccccga tactaacttc atacttactc tggtggattc tgccggtcaa aattctacga
     4981 actttaccat tccggcaaat tcgaccgtta ctttccgtta ccgcgcgcag ttcaatcgga
     5041 gttcggacga taccgtccac cgcgcggacc gaacccgcgc ctttgcctgg caactgcgcg
     5101 gtaattccat cactaccccg cttttgtcgc aggcttttac cacctttgtc gaacatgtcc
     5161 gacctcagtt tgtccagccg gttcagaatt cctttacccg gtctgcctgt gatttctctt
     5221 tcgatttcct ggtgacggca atggatattg acgaggaaat attgttcccg ctcaatatcc
     5281 gttactgtgg tacttgcttc cgggagacgg tcagtctgcg ggctccggtt aacttaccaa
     5341 catttttccc ggataccgtg gctgctcttg gcacaaacaa cccggggaga tttcgctgga
     5401 cgccgggcgt cgcggacaca ggtgtctggc tggcgaaatt cgtcgcacgg gatagcgtgg
     5461 acaagatgga agataccacc gatattttca tctccgtcag tgcgacctca gccaccggag
     5521 tgattgcagt gaggcatatc gtggtcaccg ttccggagag caccacctgt ttttcggtag
     5581 cggtgcgggt tttcaatgct gattttctcc gaacgatcaa aatccccctt gtctacgatt
     5641 cgttaatttg tctgaaagcc atcacatacg acagtacccg tttagcaaat tcaaacattt
     5701 tgaaccaacg attcctcgac agcacaagct acaggaacca caatggtctg gatacgttgg
     5761 tgttaacctt tttcagaacc gtcgggttta gtgatctgga gcccggttgt ggaacgattg
     5821 ccaatatttg gttcagcact aaagtaactg acacaactgt caacccactg gacaccgttt
     5881 tcattaactc gtcagagcgt cccttcttcg aaactgccgg aggccaggag gttctcaatc
     5941 tccaatttca aaacgaaccg ttgcatccgc aagtactcct aggagatttg gattgcgaca
     6001 cggtaagaga catcaatga
//
LOCUS       DBNGZD010000055        11620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_771950, whole genome shotgun sequence.
ACCESSION   DBNGZD010000055 DBNGZD010000000
VERSION     DBNGZD010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_771950"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1492
                     /gene="nusA"
                     /locus_tag="AC16GM_01555"
     CDS             140..1492
                     /gene="nusA"
                     /locus_tag="AC16GM_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR2922393.1"
                     /translation="MSADIMDALQQIAHEKNIELDYVVETLEVGLLTAAKKKYGNADN
                     ITVKLNRRSGQIDVVAKKKVVAAVADAALEIAVADARALNPDVNEGEEIDIPLPLEEF
                     GRNAIAAAKQTLIQRVREAEREKIYNEFIQRVGEVVSGHVQQIERGNLIINLVKTEAY
                     LPLKEQIPRERYRQGDRIRALITDVQKTARGPQIMLSRIHPDLLVRLFELEVPEIFER
                     IIEIKAVAREPGERSKIAVSSADERIDPVGACVGMKGSRVQAIVRELNNERIDIIPYH
                     SDPELFVSRALSPAKTLSLQAATDEHRMDIVVADDQLSLAIGKNGQNARLAAKLTGWK
                     VNITSESEFNARKKARREEKIDLRKLAGIGDKVGKKLMEQGIDSVQTLAAASVEQLVE
                     VEGIGEKKATKLIAQAQTYLQEAGKVPPPGEEEGTALAEEKAVAETGGPDEKEQATEE
                     "
     gene            1495..3786
                     /gene="infB"
                     /locus_tag="AC16GM_01560"
     CDS             1495..3786
                     /gene="infB"
                     /locus_tag="AC16GM_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR2922394.1"
                     /translation="MAAKRIYEIAKEYKVSSEAMMAIIKKLNIPVKSHMATASEELLA
                     AIALHFEKEKDRVKQDYAAKRQKALERQKVVPPPPPPVPLTVPEPAKAVPGAAPAKTP
                     PPPKAPGQPAPPPPRPVYRPIESFRPRSQRDTRRRPFDRPVDQNAVQASFRRTMAELG
                     THRKSRRSFHREETPEAVTVEPAKKVRVNEYMTVGELALAMQLRPAQVIAKCLELGLL
                     ASINQRLDLDTIQTLALEFGFETDQVKEIGVEESAQEEEGELLPRPPVVTIMGHVDHG
                     KTSLLDYIRKSNVIAGESGGITQHIGAYEVELPGGKITFLDTPGHQAFTAMRARGAQI
                     TDIVVLVVAADDSVMPQTVEAIDHARAAGVPIIVAINKIDLPTARPEQIRQQLSKYNL
                     VPEEWGGKTIMVDISAKIGKNIERLLEMILLQAELLELKAAPFQRGSGIIVESQLDRG
                     KGPVTTVLVQKGTLRVGDPFVCGQYHGRVRALLNERGHPGEFAGPSTPVQVLGAAGVP
                     QAGDSFFVTADEQKAREIAGKRQQVKREQEFHRIQSITLSDVYQKVKAGQMQELRVII
                     KADVDGSAEVLADTLEKIGSSEVKVTVIHRGVGAINESDVLLAVASQAIIIGFHVRPE
                     LRARELAARERIDIRLYTIIYEVEQEIKKALEGMLAPEKSEQVNATLEVRQLFKVPKA
                     GVVAGSYVQSGTVKRTDRIRVLRDGMAVYDGVIGSLKRFKDDVREVPAGYECGLRVEN
                     FNDLKVGDTLETYEITEVARKLE"
     gene            3752..4078
                     /locus_tag="AC16GM_01565"
     CDS             3752..4078
                     /locus_tag="AC16GM_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR2922395.1"
                     /translation="MKSPKWRGNWSDAVDLVVGRATLDLHLPESASLKGKRHLLQRIK
                     ERVRSRFNVSVAEVGSNDLWQRSQLAIAVVANDQAFANAVLSKVVGQVQAEAGVVLLD
                     YTFDFS"
     gene            4094..4459
                     /gene="rbfA"
                     /locus_tag="AC16GM_01570"
     CDS             4094..4459
                     /gene="rbfA"
                     /locus_tag="AC16GM_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR2922396.1"
                     /translation="MQFKRSERVGDLIHRETADIIDNLLKDPDKGFSTVTAVEVTDDL
                     RMAKIFVSCYGSAEQQKKSLQALQRAARFVHTELGRRLRLKFTPQVIFCPDRSVEEGL
                     KIDRLLREVAPKNREEENL"
     gene            4440..5441
                     /locus_tag="AC16GM_01575"
     CDS             4440..5441
                     /locus_tag="AC16GM_01575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR2922397.1"
                     /translation="MKKRIFNQMDQMAHRAERILVTAHSDPDGDSIGSQLALADYLRS
                     RKKKVRVFNQGRMPTKYRFLDPQRRLGEFHPTLKFDPDLAFVIECPSLERIGKVRHLL
                     GPTCPIINIDHHPENERFGQINWLDTRAAAVGEMIYEFLVAARHPISPAVATLLYTAI
                     LTDTGRFRYGSTRPQTLSICAALMAQGADPKVITDEVYFNHPPANLRLIGQVVRNLEL
                     AYGKRVCALSIRRADLERFGAHFEDTEGIVDYSLFAKGVAVGLLFKEVTPRKTKVSLR
                     SQNHIDISRVARKFGGGGHANAAGCSVAAPLETAKKQVLKEVIWYLRNGAGRNALNR"
     gene            5425..6336
                     /gene="truB"
                     /locus_tag="AC16GM_01580"
     CDS             5425..6336
                     /gene="truB"
                     /locus_tag="AC16GM_01580"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR2922398.1"
                     /translation="MLLIDKPSGPTSHDVVDRVRAILKEKRVGHTGTLDPLASGLLVI
                     CVGRATKVARYLAGEDKEYQAVIRLGVTTTTQDRTGEKIQTRPVEGVTRADVEAALPQ
                     FIGLQKQTPPAFSAVKVQGVPAYRSARRGEPVALTPREIEVFELQLTACEMPLVTLRI
                     RCSKGTYVRALAAEIGERLGTGAHLAELRRTKVGPWDVAEALTVDQLQQAAQEQKISA
                     RLIPLARALPLSKLSVSAALAERIGSGPLLTRENLLGIEGDFAPGDPVVLVDPAGLAI
                     AVGEVLYAKAELFYRAGPLFRYHRVLV"
     gene            6333..7289
                     /locus_tag="AC16GM_01585"
     CDS             6333..7289
                     /locus_tag="AC16GM_01585"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR2922399.1"
                     /translation="MNIIHDTCQNEKKYFAASVVTLGTFDGVHRGHQELLRQITSRAG
                     KARLPAVLVTYHPHPVRVLRPAEAPPELTTLDEKLPLFELAGISDCIVMQFDQTLAHL
                     TALDFIERILVRKLGAREVTVGYDHRFGHQRTGTIDLLRQAGERWGFQVTVVEPVADG
                     SQPVKSSHIRQALQAGDMPTANLLLGHDFSLTGEIVPGHGVGKTMGYPTINLKLPPEK
                     IVPRYGIYASRVDLDGQVHYGLLYIGNRPTFPGALLSVEVYVLDYHNQERPARVVVSP
                     QVYLREDRKFPGPEALAAQMRKDEELVRSRFIPDTVKMTERR"
     gene            7314..7565
                     /gene="rpsO"
                     /locus_tag="AC16GM_01590"
     CDS             7314..7565
                     /gene="rpsO"
                     /locus_tag="AC16GM_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR2922400.1"
                     /translation="MKTETVRQYQKHPDDTGSPEVQIALLTQRINDISQHLAKSQKDF
                     HARRGLMRMVGQRRRLLDYLHATDVESYRKLIEKLDLRK"
     gene            7588..9774
                     /gene="pnp"
                     /locus_tag="AC16GM_01595"
     CDS             7588..9774
                     /gene="pnp"
                     /locus_tag="AC16GM_01595"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR2922401.1"
                     /translation="MIHSVEKEIGGKRLVIETGRVARQADGAVTVRLEDTVILATAVA
                     AREVTPDQDFFPLTVDYREKAYAAGKIPGGFFKREGRPREKEITSSRQIDRPLRPLFP
                     EGFFNEVQIMVSVLSSDQANDADVLGIIGASTALAISPIPFRTPVGAVRVGRIQGQLV
                     LNPTFAELDESDLNLVIAATKESIVMVEGGGREVGEADLVAALAFGHQAAKEIIALQE
                     ELQRQVGQPKWPVTPPAHNPELLAKVEQLAAERIRQANAIVDKQNRQAEIERITAEVK
                     TELAAAFPESEKQIAQILWDIEKREVRSLILDASRRVDRRKFDEIRPITCEVGYLPRT
                     HGSALFTRGQTQSLAVVTLGSKMDEQKLDELEGESYKSYMLHYNFPPFSVGEVRPNRG
                     PGRREIGHGVLAERAIQPVIPKEEQFPYTVRIVSDILESNGSSSMATVSSGSLALMDA
                     GVPIKAPVTGIAMGLVIEGGRYAILSDIQGVEDHLGDMDFKVAGTRQGITAFQLDIKV
                     PGITLEIMEKALRQAQAGRLFILEAMEKTIAQPRAALSPYAPRIIIFKIKPDKIGDVI
                     GPGGKTIRSIIEKTGAKIDIEDDGSVFISSVDQKAGELAQEMVLELVQEAEIGKVYTG
                     KVRRVTAFGAFVEIIPGQDGLVHISEIDTQRIARVEDVMNVGDMVTVKVIGIDEEGKI
                     RLSRKALLAPAAGDQEPSHERRHGHERSGHRSDRGSGPGRSHAHRR"
     gene            9781..11055
                     /locus_tag="AC16GM_01600"
     CDS             9781..11055
                     /locus_tag="AC16GM_01600"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2922402.1"
                     /translation="MLQSLYKKTVLDSGLRIVTEYIPQVRSVSLGIWVNVGSRDERPQ
                     HSGISHFIEHMLFKGTKKRSAREIADALESVGGSINAFTSREQTCYYAKVLDEHLPVA
                     VEVLADILKNSLLVPADLEKEKKVISEEIKDVQDSPSDLIHDLFAESIWEGHQLGRPI
                     MGSVQSVTRLARRDLVAYLRSHYVAPAVVVAACGNVSHRKVVRLVEREFDFSANGKGN
                     GRVRTRPRAKNRREVKPRETSQTHVCLGVPALPFRDEARHALLILNTILGGGMSSRLF
                     QSVREKHGLAYTIYSFQEFYQDTGLLGIYLGTDNRQVAQAIELVLRELRKFRREKLSK
                     SELAAAKSQLKGSLMLGLESTSNRMNRLARHELFLNEYVSLDDTIRAINKVKSRDVTL
                     LAQRLLDEESLAAAVLGPNHDSLIEKIDWSLS"
     gene            11113..>11620
                     /locus_tag="AC16GM_01605"
     CDS             11113..>11620
                     /locus_tag="AC16GM_01605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2922403.1"
                     /translation="MAVLGVLALLGLAGALALFAVWHLVYRRVAAPPAPVFVCHRVSS
                     RFDLSISRQSSQNFLRICQTLAFLGYRGVTLSEALSQSPDAHIFALTFDDGYECFLRE
                     AYPVLQKFGWPATLFVVTGFVGQTSEWDLRWGWPRPRHLTWEQIEELHESGIEIGSHG
                     VTHCDLTRL"
BASE COUNT         2533 a         3468 c         3541 g         2078 t
ORIGIN      
        1 ttccgatcta agagcagcga cgaggtggcc gaactcctgg tggacgggga gccggttgta
       61 gtcgaactgg cccgcatcac tagggcgcag gtgatcgtgt gaaccggaga ggcggaacat
      121 tgttgcaaaa ggaaggccga tgagcgcgga tatcatggat gcgctgcagc agattgcgca
      181 cgaaaaaaat attgaactgg actacgtcgt cgagaccctg gaagtagggc ttttgaccgc
      241 ggccaaaaag aagtacggca acgcggacaa cattacggtc aaactgaacc ggcgttcggg
      301 acagatcgac gtagtcgcca agaagaaagt cgtggcggcg gtcgccgacg ccgctctgga
      361 aattgccgta gccgacgccc gagcgttgaa cccggacgtg aacgagggag aggaaatcga
      421 cattcccctc cccctggaag aattcgggcg caacgccatt gcggcggcca agcagacctt
      481 aatccaaagg gtgcgggaag cggagcgcga aaaaatctat aacgagttca tccagcgggt
      541 gggggaagtg gtctccggcc acgtccagca aattgagcgg ggcaatttga ttatcaacct
      601 ggtcaagacc gaagcctacc tgccgctcaa agaacagatt ccgcgggagc gctaccgtca
      661 gggcgaccgg atccgggcgc tcattaccga cgtgcagaag acggcccgcg gcccccagat
      721 catgctctcc cggatacacc ccgatctcct ggtacggctg ttcgaactgg aagtgccgga
      781 aatcttcgag cgcattatcg agattaaagc ggtggctcgg gaacccgggg aacgctccaa
      841 aatcgccgtc tcttcggccg acgaacggat cgatccggtg ggcgcctgcg tcggcatgaa
      901 agggtcccgg gtgcaggcga tcgtccggga gttgaacaac gaacggatcg acatcatccc
      961 ctaccactcc gatccggaac tcttcgtctc gcgcgccctc tcgccggcca agacgctctc
     1021 cctccaggcc gccaccgatg agcaccgcat ggacatcgtg gtggccgacg atcagctctc
     1081 tttggccatc ggcaagaacg gacagaatgc ccgcctggcg gccaagctca ccggctggaa
     1141 ggtcaacatc accagcgaat cggagttcaa cgcgcgcaag aaggcccgcc gcgaggagaa
     1201 aattgacctg cggaaactgg ccggcatcgg cgacaaagtc ggcaaaaagc tgatggagca
     1261 gggcattgat tcggttcaga cgttggcggc tgccagcgtg gaacaattag ttgaagttga
     1321 aggcatcggc gagaaaaaag ctactaaact gattgcgcaa gcgcagacct acctccagga
     1381 agccggcaaa gttcctccgc cgggagaaga agaagggact gctcttgcgg aggagaaagc
     1441 ggtggcggag accggcggac cggacgagaa agaacaggca accgaagagt aaccatggcg
     1501 gccaaacgga tttacgaaat cgccaaagag tacaaggtct ccagcgaggc catgatggcg
     1561 attatcaaaa aactgaatat cccggtcaaa agccacatgg ctaccgcctc ggaagaactt
     1621 ctggccgcca ttgcgctcca ctttgagaaa gagaaagacc gggtgaaaca ggattatgcc
     1681 gccaagcggc agaaagcgct ggagcggcag aaagtggtgc cgccaccacc cccccctgtg
     1741 ccgctgaccg taccagagcc cgccaaggcc gtccccggag cggcgcccgc aaaaacaccg
     1801 ccgccgccaa aagcgcccgg tcagcctgca cccccgccgc cgcgccccgt ctaccgaccc
     1861 attgagagtt ttcggccgcg gtcgcagcgc gatacccggc ggcggccttt cgaccgtccc
     1921 gtcgaccaga acgcggtgca ggccagtttc cgccgcacca tggccgaact cggcacccac
     1981 cgcaagagcc ggcgttcctt tcaccgggaa gaaactcccg aagcggtcac ggtcgaaccg
     2041 gccaagaagg tgcgggtcaa cgagtacatg accgtgggcg aactggcgct ggcgatgcaa
     2101 ttgcgcccgg ctcaggtgat tgccaaatgc ctcgaactgg ggcttttggc ctcgatcaac
     2161 cagcgcctcg acctggacac gattcaaacc ctggctctgg agtttggctt tgagaccgac
     2221 caggtgaaag aaatcggtgt cgaggaaagc gcccaggaag aggaagggga acttttgccc
     2281 cgcccgccgg tcgtgaccat tatgggccac gtcgatcacg gcaaaacgtc ccttctggac
     2341 tacattcgca aaagcaacgt catcgccggt gagtcgggcg gcatcaccca gcacatcggg
     2401 gcttacgaag tcgaactccc cggcggcaag atcacctttc tggacacgcc cggccaccag
     2461 gcctttaccg ccatgcgggc gcgcggcgca caaattaccg acatcgtcgt cctggtggtg
     2521 gcggccgacg acagcgtgat gccccagacg gtggaggcga ttgaccatgc ccgggctgcc
     2581 ggcgtgccga tcatcgtcgc tatcaataag attgaccttc ccaccgccag accggagcag
     2641 atccgccagc aactgtccaa atacaatctg gtgcccgaag agtggggcgg aaaaacgatt
     2701 atggtggaca tctccgccaa aatcggtaag aacatagaac ggcttttaga aatgattctc
     2761 ctccaggcgg aacttctgga actgaaagcg gcgccgttcc aacggggctc cggcattatc
     2821 gtcgaatccc agttggaccg gggcaaagga ccggtgacga cggttttagt gcagaagggg
     2881 accttgcggg tgggagaccc ttttgtgtgc ggccagtacc atgggcgggt gcgtgcgctt
     2941 ttgaatgagc gcggccaccc cggcgaattc gccgggccct cgactccggt gcaggtattg
     3001 ggggctgccg gcgttccgca ggccggtgac tccttcttcg tcaccgccga tgagcagaaa
     3061 gcgcgcgaga tcgccggcaa acggcagcag gtcaagcggg agcaggaatt ccaccgcatc
     3121 cagagcatca ccctctcgga cgtctaccag aaggtcaaag ccggccagat gcaggaactg
     3181 cgggtcatca ttaaagccga cgtcgacggc tccgccgaag tgctcgccga tacgctggaa
     3241 aaaatcggct cgtccgaagt gaaggtgacc gtcatccacc gcggcgtcgg cgccatcaat
     3301 gaatccgacg tgctcctggc agtcgcctcc caggccatca tcatcggctt tcacgtgcgg
     3361 cccgaactcc gggcgcggga attggccgcc cgggagcgga tcgacatccg gctctatacg
     3421 atcatttatg aggtggagca ggaaatcaaa aaggcgctgg aggggatgct cgctccagag
     3481 aaatcggagc aggtgaacgc caccctcgaa gtgcggcagc tgttcaaagt acccaaggcc
     3541 ggcgtcgtcg ccggctccta cgtccagagc ggcaccgtga agcggaccga ccggatccgg
     3601 gtgctccgcg acgggatggc cgtctacgac ggggtgatcg gctccctcaa gcggttcaag
     3661 gacgacgtgc gcgaagtgcc ggcggggtac gagtgcggac tgcgggtcga aaatttcaat
     3721 gatctgaaag tgggcgacac gctcgaaacg tatgaaatca ccgaagtggc gcggaaactg
     3781 gagtgacgcc gtcgatctcg tggtgggccg ggcgacgctc gacctgcacc tccctgaatc
     3841 ggcttcgctt aaggggaagc gtcatctcct gcagcggatc aaggagcggg tacgcagccg
     3901 cttcaacgtc tccgtcgccg aggtcggctc caacgacctc tggcagcgca gccagttggc
     3961 cattgccgtg gtggccaacg accaggcgtt cgccaacgca gtgctctcca aggtcgtagg
     4021 ccaggtccag gcggaagcgg gtgtggtgct tctggattac acatttgatt tcagttgagc
     4081 cgagagaaac cgtatgcagt tcaagcgttc cgagcgcgtc ggcgacctga tccaccgtga
     4141 gaccgcggat attattgaca acctgctcaa ggatcccgac aaggggttct cgaccgtcac
     4201 ggctgtggag gtaaccgacg atctgcggat ggcaaaaatt ttcgtttcgt gctacggctc
     4261 ggccgagcag caaaagaaga gtttgcaggc cctccagcgg gccgcaaggt tcgtacacac
     4321 cgaactgggt cgccgcctgc gcttgaagtt cacgccgcag gtaatctttt gcccggaccg
     4381 ttcggtggag gaagggttga aaatcgaccg cttgctccgg gaagttgccc cgaagaaccg
     4441 tgaagaagag aatctttaac cagatggacc agatggcgca ccgtgcggag cgcattctgg
     4501 tcacggccca cagcgacccc gacggcgact ccatcggctc gcaattggct ttggccgact
     4561 acctgcgctc ccggaagaaa aaggtgcggg tgttcaacca ggggcggatg cccaccaagt
     4621 accggttcct cgatcctcaa aggcgcctgg gagaattcca tccgaccctg aagttcgacc
     4681 cggatctcgc ttttgtcatc gagtgtcctt cgctcgaacg gatcgggaaa gtaaggcatc
     4741 ttttagggcc gacctgtccc atcatcaaca tcgaccacca cccggagaat gaacggttcg
     4801 gtcaaatcaa ctggctggat acgcgggcgg cagcggtagg ggagatgatt tacgaatttc
     4861 tggtcgccgc ccggcacccg atttccccgg cggtggcgac cctcctctac acggcgattc
     4921 tcaccgacac cggccgcttc cgctacggca gcacccgccc gcagaccctt tcgatttgcg
     4981 ccgctctgat ggcgcagggc gccgacccca aggtgatcac cgatgaggtc tattttaacc
     5041 acccccccgc caatttgcgc ctgatcggac aggtcgttcg caatctggaa ctggcctacg
     5101 ggaagcgcgt ctgcgccctc tccatccgcc gggccgatct ggagcggttc ggagcgcact
     5161 ttgaagatac cgagggtatc gtcgattatt cccttttcgc caaaggcgtg gccgtgggac
     5221 ttttgttcaa ggaagtaacc ccgaggaaaa cgaaggtcag tctccgctcc cagaaccaca
     5281 tcgatatcag ccgggtggcg cgtaaattcg gcggcggcgg gcacgccaat gccgcgggct
     5341 gcagcgttgc ggccccgctg gagacagcca aaaagcaggt tttgaaggaa gtcatctggt
     5401 acctccgcaa tggcgcagga cggaatgctc ttaatcgata aaccctcggg ccccacctcc
     5461 cacgacgtgg tggatcgggt gcgggccatc ctgaaagaga agcgcgtagg ccatactggc
     5521 acgttggacc ccctggcctc cgggcttctg gtcatctgtg tcggccgggc caccaaagtc
     5581 gcccgctacc ttgcgggcga agacaaagaa taccaggcgg tgattcgtct cggggtaacc
     5641 acgacgaccc aagaccgtac cggagaaaag atccagaccc gacctgtgga gggcgtaacc
     5701 cgggccgatg ttgaggccgc cctcccgcaa ttcatcgggt tgcaaaagca aaccccgccg
     5761 gccttctcgg cggtgaaagt gcaaggagtg ccggcctatc gttccgcccg gcggggtgag
     5821 ccggtggcgc tgaccccgcg tgaaatagaa gtgttcgaat tgcaattgac cgcttgcgaa
     5881 atgcctctgg tcacgctccg catccgctgc tccaaaggca cctacgtgcg cgcgctggcg
     5941 gcggaaatcg gcgaacgttt gggaaccggc gcgcatctgg ccgagctacg acgtacaaaa
     6001 gttggacctt gggacgtggc cgaagcgctc accgttgacc agttgcagca agccgctcag
     6061 gagcaaaaga tctccgcccg actgatcccg ctcgctcgag ccttgccctt gtccaaactc
     6121 tccgtctccg ccgcacttgc ggaaagaatc ggtagtgggc cgctattgac gcgagaaaat
     6181 ctcttgggaa ttgaggggga cttcgctccc ggcgacccgg tggtgctggt tgatccggcc
     6241 gggctggcca ttgccgttgg cgaagtactt tatgcgaaag ccgaactgtt ttatcgggcc
     6301 ggaccgcttt ttcgttatca ccgcgtactc gtatgaacat cattcacgat acttgtcaga
     6361 acgaaaaaaa atactttgcg gcctcggtgg ttaccctggg cacttttgac ggcgtccacc
     6421 gcggccacca ggaactgctc cggcagataa cgagccgggc cgggaaagcc cggctccccg
     6481 ccgtactggt cacctaccac ccccatccgg tgcgggtcct ccggccagcc gaagccccac
     6541 cggaacttac caccctggac gaaaaactcc cgctgttcga actggccggg atttcggact
     6601 gtatcgtgat gcagttcgac caaacgttgg cccacctcac ggcacttgac tttatcgagc
     6661 gtatccttgt tcgaaaatta ggagcccggg aggtcaccgt cggctacgat caccgtttcg
     6721 gccaccagcg caccggaacc attgacctgt tgcgccaggc cggcgagagg tggggatttc
     6781 aggtgacggt ggttgagccg gtagccgacg ggagccagcc ggtgaagtcg agccacatcc
     6841 gtcaagccct ccaagccgga gatatgccaa ccgccaacct tctcttgggg cacgatttct
     6901 ccctcaccgg agaaatcgta ccaggccacg gcgtggggaa gaccatggga tatccgacca
     6961 tcaacctcaa gcttccgcct gaaaaaatcg ttccaagata cggcatctac gccagccggg
     7021 tggatctgga cgggcaggtg cactacggat tgttgtatat tggcaaccgg ccgactttcc
     7081 ccggtgctct cctctcggtg gaagtctacg tcctggacta tcacaaccag gagcgtccgg
     7141 ctcgggtggt agtttccccg caggtttacc tccgggaaga tcggaaattt cccggtccgg
     7201 aagctctggc ggcacagatg cgtaaggatg aagagctggt ccggagccgg ttcatcccgg
     7261 atacggtgaa aatgacagaa aggagatgag cggaggtgga gaggcccaaa gtgatgaaaa
     7321 cggaaaccgt gcggcagtac caaaagcacc cagacgacac cggctcgccg gaagtgcaaa
     7381 ttgcactgct aacgcagcgc atcaacgaca tcagccagca tctggccaaa agccagaaag
     7441 acttccacgc ccggcgtggc ttgatgcgta tggtcgggca acggcgccgg ttgttggatt
     7501 acctgcacgc gaccgatgtg gagagctacc ggaaactgat tgagaaattg gacctgcgga
     7561 agtgagagaa aggacaaagt cgagcgaatg atacattcag tcgagaagga aattggcgga
     7621 aaacggctgg tgatcgagac cgggcgggtg gcccggcagg ccgacggagc tgtcaccgtt
     7681 cggctggaag acacggtgat tctggccacc gcggtggcgg cccgggaggt gacgccggac
     7741 caagattttt tcccgctgac ggtggactac cgagagaagg cctacgcggc cgggaaaatt
     7801 cccggcggct tcttcaagcg cgagggccgg ccccgcgaaa aagaaatcac cagctcgcgg
     7861 caaatcgacc ggcccctgcg gccgctcttc ccggaaggtt ttttcaacga agtgcagatc
     7921 atggtctccg tgctctcgtc ggatcaggcc aatgacgccg atgttttggg aatcatcggc
     7981 gcctccacgg cactggccat ctcccccatc ccctttcgca ccccggtggg cgcggtccgg
     8041 gtgggccgga tccagggcca gctggtactg aatccgactt ttgccgagtt ggacgagagc
     8101 gatctcaact tggtcatcgc cgccaccaaa gagagtattg tgatggtgga ggggggcggc
     8161 cgggaagtgg gcgaagccga cctggtggcg gctctggcct tcggccacca ggcagccaaa
     8221 gaaattattg cccttcagga agagctgcaa aggcaggtgg gccagcccaa gtggcccgtt
     8281 accccgcccg cgcacaaccc ggaattgctg gccaaagttg agcaattggc tgcggaaagg
     8341 atccgccagg ccaacgccat agtcgacaaa caaaaccggc aggcggaaat cgagcgtatc
     8401 accgccgagg taaagaccga attggcagcc gcgtttccgg aatcagaaaa gcagatcgcc
     8461 cagatcctct gggatattga aaagcgtgag gtccgctccc tcattctgga tgccagccgg
     8521 cgggtcgacc ggcggaagtt tgacgaaatc cggcccatta cctgcgaagt cggctacctg
     8581 ccccgcaccc acggctcggc tctgttcacc cggggtcaga cgcagagtct cgcggtcgtg
     8641 actctgggct ccaagatgga tgagcagaaa ttagacgaac tcgaggggga atcgtacaaa
     8701 tcgtacatgc tccactacaa cttcccgccc ttctcggtgg gcgaagtccg gccgaaccgg
     8761 ggaccgggcc ggcgggaaat cggccacggc gttttagctg agcgggccat tcaaccggtc
     8821 attcccaaag aggagcaatt cccgtacacg gtccgcatcg tctcagacat cctggagtcc
     8881 aacggctctt catcgatggc cacggtctcc agcggcagcc tggccttgat ggatgccggg
     8941 gtcccgatca aggcaccggt gacgggcatt gcgatgggcc tggtgatcga aggcggccgt
     9001 tatgcgatcc tctccgacat tcagggggtg gaggaccacc tgggggatat ggacttcaaa
     9061 gttgccggca cccggcaggg catcaccgcc ttccagctgg acattaaggt cccgggaatc
     9121 accctggaga ttatggaaaa agcgctccgg caggcgcagg ccgggcgcct cttcattctc
     9181 gaggcgatgg aaaaaactat tgcccagccg cgcgctgcgc tttcgcccta tgcgccgcgg
     9241 atcataatct tcaagatcaa accggataag atcggcgacg tcatcgggcc gggcggcaag
     9301 acgatcagaa gcatcatcga gaagaccggc gccaaaatcg atattgaaga cgacggctcg
     9361 gttttcatct cctcggttga ccaaaaagcg ggcgaactgg cccaagagat ggtgctggaa
     9421 ctggtgcagg aagccgaaat cggcaaggtc tacaccggca aggtccggcg ggtaaccgcc
     9481 ttcggcgcct ttgtggaaat catcccggga caggatgggt tagtccacat ttccgaaatc
     9541 gacacccagc gcatagcccg ggtagaggac gtgatgaacg tgggcgacat ggtgaccgtg
     9601 aaagtgatcg gcatcgacga agaagggaag attcgccttt cgcgcaaggc gcttttggca
     9661 cccgccgcgg gtgatcagga accgtcccac gagaggcggc atgggcacga acggtcgggc
     9721 caccggtccg accggggttc gggcccgggc cgttcccacg cgcacagacg gtaggtcggg
     9781 atgttgcagt cgctctacaa gaaaaccgtc ctggactccg gtctgcgcat tgtgaccgaa
     9841 tacatcccgc aggtgcgctc ggtttccctg ggaatctggg tgaacgtggg ttcccgtgac
     9901 gagcgtccgc agcactccgg catctcgcac ttcatcgagc acatgctctt caaagggacg
     9961 aaaaaacgca gcgcccggga aatcgccgac gcgctggaat cggtgggcgg ctccatcaac
    10021 gccttcacct cgcgggagca gacctgctac tacgccaagg tactggacga acacctgccg
    10081 gtcgccgtcg aagtcctggc cgacatactc aagaattcgc ttctggtgcc ggcggatctg
    10141 gaaaaagaga agaaggtaat ttccgaagag attaaagatg tccaggattc accctccgat
    10201 ctcatccacg atctcttcgc cgaatcgatc tgggaaggac accagctggg gcgaccgatt
    10261 atggggagcg tccagagcgt cacccggctg gcccggcgtg acttggtggc ctacctgcgg
    10321 tcgcactacg tcgccccggc ggtggtcgtg gccgcctgcg gcaacgtcag ccaccggaag
    10381 gtagtccggc tggtggaacg ggagttcgat ttttccgcca acggcaaagg gaacgggcgg
    10441 gtgcggaccc ggccgcgcgc caaaaaccgg cgcgaggtca aaccgcggga aacgtcgcaa
    10501 acccacgtct gcttgggcgt gccggcttta ccctttcgcg atgaagcgcg ccatgccctt
    10561 ttgattctga acaccattct cggcggcggc atgagttccc gcctctttca gtcggtgagg
    10621 gaaaagcacg gtttagccta tacgatttat tcgttccagg agttttacca ggacaccggc
    10681 ctcctgggca tttacctggg aaccgataac cggcaggtgg ctcaagccat cgaactggtg
    10741 ctgcgcgaac tccgcaagtt ccgccgggag aaactttcca aaagcgaact ggccgcagcc
    10801 aagtcgcaac tcaaagggag cctgatgctc gggctggagt ccacctccaa ccggatgaac
    10861 cgcctggcac ggcacgaact cttcttaaac gagtacgtct ccttagacga taccatccgt
    10921 gccattaaca aagtgaaaag ccgcgacgtg acccttttgg cccagcgcct gctggacgag
    10981 gaaagtctcg ccgcagcggt cctcgggccc aatcacgact cgctgatcga gaaaatcgac
    11041 tggagtctgt cgtaagactc gaccctacgt atgaacgact cttacgagcg gtaatagccg
    11101 gcagaaatcg ccttggccgt tcttggggtt ctcgccctcc tgggattagc cggcgcactg
    11161 gctctttttg ccgtctggca tttggtttat cgacgggtag ccgcaccgcc cgcgccggtt
    11221 ttcgtctgcc accgcgtctc gtcccggttt gatctttcga tctcgcgcca atcgtcccaa
    11281 aattttttgc ggatttgtca aacgctcgcc tttctgggtt accgcggcgt aacgctttcg
    11341 gaagcccttt ctcaatctcc ggacgcacat atttttgctt taacgttcga cgacgggtat
    11401 gaatgtttcc tccgggaggc ctatccggtt ctccaaaaat tcggttggcc agcgaccctc
    11461 tttgtggtca ccggttttgt gggtcaaacc agtgagtggg atttgcgctg gggctggcca
    11521 cgaccgcgtc atctcacctg ggaacaaatt gaagagttgc acgaaagcgg tattgagatc
    11581 ggctcccatg gggtaacaca ctgcgactta acccggcttt
//
LOCUS       DBNGZD010000056         2171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1257738, whole genome shotgun sequence.
ACCESSION   DBNGZD010000056 DBNGZD010000000
VERSION     DBNGZD010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1257738"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="AC16GM_01610"
     CDS             <1..434
                     /locus_tag="AC16GM_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922404.1"
                     /translation="AAHKFFSAECFNKAWELIEKPHRTPEENEQMIRLNHASLWHWTQ
                     RPDCTKRNLSIGYWQAARIYTVLGQGDNARWYGRLCLEVSVAEPPFYRAYAFEALARA
                     ETIAGNKQNAREYLAQASDLAKQVSEAEERKALLADLAAID"
     gene            575..1171
                     /locus_tag="AC16GM_01615"
     CDS             575..1171
                     /locus_tag="AC16GM_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922405.1"
                     /translation="MPLNSADPPDVRAQAAEDLRFIRETLAKAGSFTAVPGWGMVAMG
                     ISALAATVVAAARSSSAEWLVVWLVEALLAISVALLSLRRKAQRANVPVFSGPAARFL
                     LSLCPPLFAGALLTAVFYRFDLVTLLPSLWLLLYGTGIVTGGAFSIRIVPILGLCFML
                     LGVAALFTPTSWGNFFMGAGFGGLHVVFGTIIARRHGG"
     gene            1164..1556
                     /locus_tag="AC16GM_01620"
     CDS             1164..1556
                     /locus_tag="AC16GM_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR2922406.1"
                     /translation="MGRESVLVKDRSRNAPEESRLLRAVRNILSKSDAKRSDHLIHER
                     MRLGILSALAVNASLSFNDLKKLLNTTDGNLSVHARKLEEAGYIRCSKYFVKRVPKTE
                     FRLSAVGRRALERYLDEMEKLIAAMRRQ"
     gene            1621..>2171
                     /locus_tag="AC16GM_01625"
     CDS             1621..>2171
                     /locus_tag="AC16GM_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922407.1"
                     /translation="MQPYFNFQGSVVFGDTAWFGYLAFLGSGLALVAAAVTFIVLLIA
                     RKKQLARIVPLVVLIGAAAYLGTLLLFSLTSRVQVLNLNEEKYFCEVDCHLAYSVLSV
                     TTTKTLGSPPNQSTAQGVYYVVTLKVRFDETTIAKWRPKDVPLRPNPRTVAVLDDQGK
                     GYEISPEGLRALESARGAQVPLT"
BASE COUNT          461 a          607 c          627 g          476 t
ORIGIN      
        1 aggccgcgca caagttcttt tccgccgaat gcttcaacaa agcgtgggaa ttgatagaga
       61 agccccaccg cacgcccgaa gaaaacgagc aaatgatccg gttgaaccac gcctcgctgt
      121 ggcattggac ccagagaccc gactgcacca agagaaacct gtcaatcggc tactggcagg
      181 cagcgcggat ttataccgtg cttggtcagg gcgacaatgc ccgctggtat ggccgcctct
      241 gccttgaagt aagcgttgct gaaccccctt tctaccgcgc ctacgctttc gaagcgttag
      301 cccgcgcgga aacaatagcc ggaaataaac aaaacgcacg ggagtatttg gctcaagcca
      361 gtgatctcgc taaacaagtt tcagaggccg aggagaggaa agcgctcctg gccgacctgg
      421 cagccatcga ctgacgcatc ccaggcacac gcgccggtgc gtctgcctat gcactggcag
      481 cacgaaaaag aatcggttta tattttttat cataaagtac ttgactaaat tgaaattgtg
      541 ccgtatgtag taatacatgt ttgatgatcg ccctgtgcct ctgaactctg cagacccgcc
      601 cgatgtccgg gcgcaagccg ccgaagacct gcgtttcatc cgcgaaacgc tggcaaaagc
      661 ggggtcgttc acggctgttc cgggttgggg gatggttgcc atgggaattt cggccctggc
      721 ggcaacggtg gtggcggcgg ctcgctccag ttccgcagaa tggcttgtcg tctggctggt
      781 cgaagcccta ctggcgattt ccgtcgcgct cttgtctctg cgccggaaag cgcaacgagc
      841 gaatgtcccg gtattttccg ggccggcggc gaggttctta ttgagtcttt gcccgccgct
      901 tttcgccggg gctctcctca ccgcggtttt ttaccgtttt gatctcgtga ctttactgcc
      961 cagcctgtgg cttttgctct acggcacggg catcgtcacc ggcggcgcgt tctccattcg
     1021 gatcgttccg attttggggc tgtgcttcat gctcctgggc gtggctgcgc tcttcactcc
     1081 gacctcgtgg gggaatttct tcatgggcgc cgggttcggc ggcctgcacg tcgttttcgg
     1141 caccatcatt gcccggaggc acggtgggta gggagagcgt tctggtgaaa gaccgttccc
     1201 gaaatgcccc tgaggagtcg cggcttttgc gcgcggtacg caatattctc tcaaaaagcg
     1261 acgcaaaacg atctgaccac ctcattcacg agcggatgcg gttgggcatt ttgagcgccc
     1321 tggccgtcaa cgcatcgctc agtttcaatg acttaaaaaa actcttgaac accaccgacg
     1381 gcaatctgag tgtccacgcg cgcaagttag aagaagcggg gtacatccgg tgctccaaat
     1441 actttgtcaa acgagttccg aaaacggaat ttcggctgtc cgcggtgggg cgccgggcgc
     1501 tggagcggta cctggacgaa atggagaagc tgatcgccgc gatgcgccgg cagtaggaaa
     1561 aaatttttac agagacgctt tgcaaagcaa aggacttttc agaaaggaga ggaggtacag
     1621 atgcaaccct acttcaattt ccagggcagc gtagtattcg gggacaccgc atggttcggg
     1681 tacttagcgt ttttggggag cggtctggcg cttgtggcag cggccgtgac cttcatcgtc
     1741 ttactcatag cgcggaaaaa gcaactcgcc aggatcgttc cgctcgtcgt actcataggg
     1801 gcggcagcgt atttggggac tctcttgctg ttttccctca ccagccgggt acaggtgctt
     1861 aacctgaacg aggaaaaata tttctgcgaa gtagactgtc acctggccta ctcagtgctc
     1921 agcgtcacaa ccaccaaaac tttaggcagt ccgccgaatc aaagtaccgc tcaaggggtc
     1981 tattacgtag tgacgctcaa ggtccgcttc gacgagacta cgatcgccaa atggcgtccc
     2041 aaggatgtgc ccttgcggcc aaatccccgg acggtggccg tcctcgacga ccaagggaaa
     2101 gggtatgaga tttcaccaga gggtctacga gccctggagt cagcccgggg cgcgcaggtg
     2161 ccactcacca a
//
LOCUS       DBNGZD010000057         3337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1813835, whole genome shotgun sequence.
ACCESSION   DBNGZD010000057 DBNGZD010000000
VERSION     DBNGZD010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1813835"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="AC16GM_01630"
     CDS             <1..921
                     /locus_tag="AC16GM_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HIR2922408.1"
                     /translation="TNLCVLSCKFCDFAAKPGEAHAYEMTLEEILNKVTDELREVHIV
                     GGLHPTWRFEHYLEMMRAIKTKCPTVQLKAFTAVEVDFFAKMSKLTLEQTLRKLLDAG
                     LDTLPGGGAEVFSPRVRKELFKQKIGEQRWFEVHRVAHRMGIRSNATLLYGHIETYEE
                     RVDHLLKLRAVQAETGGFLTFIPLAFQPGTTGIVQRQTSALEDLRTLATSRLILENVP
                     HIKSYGVMLGEETASVGLHFGADDLDGTIGEEKIAHLALASSPVGLTRQALLEMIKEA
                     GKIPVERDALYNILKVYNGTADSQNSLPQR"
     gene            881..1648
                     /locus_tag="AC16GM_01635"
     CDS             881..1648
                     /locus_tag="AC16GM_01635"
                     /EC_number="4.2.1.151"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthetic enzyme MqnA/MqnD family
                     protein"
                     /protein_id="HIR2922409.1"
                     /translation="MGLRIAKIPYLNAEPFYAGLSATEFSLLEIPPRRLGELARAGEI
                     EAGLMPVVDFFALEKHFVPLADLGIAVRGAVKSVVLFSKVPREKLSGRKIGITPETST
                     SYRLLQLLCRGFWRVAPSAFIRDAAAISSEADVYLWIGNEVLRRNHGRSPEFPYQYDL
                     ALEWWRWQQKPFVFALWAIHRKVTTLERTRLAQVLEGSLDETSGQFEKVAQRLSGVLG
                     TTAELTEYLSNFTYRLGRDEWQGLETFRRLLDEFRIA"
     gene            1629..2714
                     /gene="mqnC"
                     /locus_tag="AC16GM_01640"
     CDS             1629..2714
                     /gene="mqnC"
                     /locus_tag="AC16GM_01640"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR2922410.1"
                     /translation="MNSVSLDAIRKKVLAGERITPAEGISLMREDDLLSVGDLAGQVR
                     WRRHPEPVVTFVIDTNPNYTNVCYIDCIFCAFYRHEGDPDAYTLSVDQVLEKVKWAEE
                     QGATTVLLQGGVNPALPLDYYLDIVRRMRQEFPRMHPHFFSAVEIQGMASVSGLSIRE
                     VLLKLKEAGLATLPGGGAEILADRVRMKLGKKKGMSNAWLEVHREAHQLNFKTTATMM
                     FGHIETDAELIEHLERIRALQDETGGFTAFIPWSFKPGNTPLEKRYPHHAGPNRYLRI
                     LAVARIFLDNFPHIQASWFSEGKKTGQVALHFGADDFGGTIFEENVHKCAGFVNTTTT
                     DEVVALIQEAGFRPAQRTTLYEIIKYL"
     gene            2773..3285
                     /locus_tag="AC16GM_01645"
     CDS             2773..3285
                     /locus_tag="AC16GM_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR2922411.1"
                     /translation="MKRVTVLTVGPVFITLLAAFAHAQEAQKPEAVAKSQHVMLTESD
                     LKWTDPAALPAGAKLAVLEGDPTQVGPFTMRLKFPAGYKIPAHWHPVDEHVTVLSGAF
                     NMGMGDKLDEKASRALPVGSFVVMPAKTNHFAWTKDGATVQLHGMGPWGITYVNPADD
                     PRIQKATTTK"
BASE COUNT          728 a          912 c         1013 g          684 t
ORIGIN      
        1 accaatctct gcgtcctttc ctgcaaattt tgcgacttcg ccgccaagcc gggtgaagcg
       61 catgcgtatg aaatgactct ggaggaaatc ttgaacaagg tcaccgacga actccgcgag
      121 gtgcacatcg tcggcgggct gcaccccacc tggcgcttcg agcattattt agaaatgatg
      181 cgggccatca aaaccaaatg tccgacggtg caactgaaag ccttcaccgc agtggaagtc
      241 gatttttttg ccaagatgag caaactgacg ctggagcaga cgctccggaa acttttggac
      301 gctgggctgg acaccctgcc tggcggcggg gccgaagtct tctccccgcg cgtgcggaag
      361 gaactcttca agcaaaaaat cggcgagcag cgctggttcg aggtgcaccg ggtagcccac
      421 cgaatgggta ttcgctccaa cgccacgctt ttgtacggcc acattgaaac ttatgaagag
      481 cgggttgatc acctcttgaa attgcgggcg gtgcaggcgg agacgggcgg tttcctcacg
      541 ttcattccgc tggcatttca gccgggcact accggcatcg tgcagcgtca gacctcggct
      601 ctggaggacc tgcggacgct ggccacctcg cggttgatcc tggaaaacgt cccgcacatc
      661 aagtcgtacg gggtgatgtt gggggaggag acggcgtcgg tggggttgca cttcggtgcg
      721 gacgatttag acggcaccat cggcgaggag aagatcgccc acctggcctt agcgtcgagt
      781 ccggtcggct taacccgtca agcgcttctg gagatgatca aagaagcggg gaaaattccg
      841 gtggaacgcg acgcgctcta caacattttg aaggtctaca atgggactgc ggatagccaa
      901 aattccttac ctcaacgctg agccgtttta cgccgggctg agcgctacgg agttctcgct
      961 tctggaaatc ccgccccggc ggctggggga gttggcgcgt gcaggtgaaa tcgaggccgg
     1021 cttaatgccg gtggtcgatt tctttgcgct ggaaaaacat tttgtgcctt tggccgacct
     1081 aggcattgcg gtccgaggag cggtcaaaag cgtggtgtta ttttccaaag tgcctcggga
     1141 aaaactctcc gggcggaaga tcggcattac tcccgagacg tcaacttcct accgtctctt
     1201 gcagctcctc tgccggggct tttggcgggt ggcgcccagc gccttcatcc gggatgcggc
     1261 tgcaatttca tccgaagccg acgtctacct ctggattggc aatgaggtct tacgccgcaa
     1321 ccatggaaga agtccggagt ttccctacca gtacgatctg gcgctggagt ggtggcggtg
     1381 gcaacaaaag ccattcgttt ttgcgctctg ggccatccac cgaaaggtca cgactttgga
     1441 acgcactcgg ctggcgcagg tgctggaagg ctcgctggat gaaaccagcg ggcagttcga
     1501 aaaagtcgcg cagcgattga gcggggtcct cggcacgacg gctgaattga cggagtatct
     1561 ctccaatttt acctaccgcc tgggtcggga cgaatggcag ggtttggaaa cctttcggag
     1621 actgctggat gaattccgta tcgcttgatg ccatccgtaa gaaagtatta gcaggggaga
     1681 ggatcacccc ggcagaggga atctccctga tgcgggaaga tgacctcttg agcgtcggtg
     1741 acctggccgg ccaggttcgc tggcggcggc acccggagcc ggtggtgacg ttcgtcatcg
     1801 atacgaatcc gaattatacg aacgtctgtt atattgattg catcttctgc gctttctacc
     1861 gacacgaggg tgatccggac gcttacacgc tttcagtcga tcaggttctg gaaaaagtca
     1921 aatgggcgga ggagcaaggt gccaccacgg tcctcttgca gggcggcgtc aatccggctt
     1981 tgccgctgga ctactattta gacatcgtgc ggcggatgcg gcaagagttt ccccggatgc
     2041 acccgcactt cttttccgca gtggaaattc aaggcatggc cagcgtgtcg ggtttatcga
     2101 ttcgggaagt cctgctgaaa ttgaaagagg ccggactggc gacgctgccc ggcggcgggg
     2161 ctgaaatctt ggccgatcgg gtgcggatga aattaggcaa gaagaaaggg atgtccaacg
     2221 cttggctgga ggtccaccgc gaagcgcacc agttgaactt caaaaccact gcaaccatga
     2281 tgttcggcca cattgaaacc gacgcggagc tcatcgagca tttagagaga atccgggcgc
     2341 tgcaggatga aaccgggggc ttcaccgcct tcattccctg gagcttcaaa ccgggcaata
     2401 ccccgcttga aaaaagatac ccgcaccatg cggggccgaa tcgctatctg cgcattctgg
     2461 cggtcgcgcg catttttctg gacaactttc cgcacatcca ggcctcctgg ttttcggaag
     2521 ggaaaaagac cggccaggtg gcgctgcact tcggagcgga cgatttcggc ggcacgatct
     2581 tcgaagagaa cgtccacaag tgcgccggtt ttgtgaatac caccacgacg gacgaggtgg
     2641 tggccctgat tcaagaagcc ggcttccgtc cggcccagcg cacgacgctg tacgagatca
     2701 tcaaatacct atagcggcgg tttttcattc gccgagagag agcggacaaa aacagtaagg
     2761 aaggagggaa aaatgaaacg cgtcacagtt ctgaccgtcg ggcccgtttt catcaccctg
     2821 cttgcagctt ttgcacacgc gcaagaagcg cagaagccgg aagcggttgc caaaagtcag
     2881 catgttatgc tgacggaaag cgacctcaag tggactgacc cggcggcgct gcctgcaggt
     2941 gccaagctcg ccgtcctgga aggagacccg acacaagttg gtccttttac catgcggctc
     3001 aagttccctg ccgggtacaa gataccggcg cactggcatc ccgtcgatga gcatgtcacc
     3061 gtgctctccg gtgccttcaa catggggatg ggtgacaaac ttgacgaaaa ggcgagtcga
     3121 gcactgccgg tggggagttt tgtggttatg ccagcgaaga ccaaccactt tgcctggacg
     3181 aaggatggtg ccacagtaca gctgcacggc atgggtccgt ggggcatcac ctacgtcaat
     3241 ccggccgacg atccgcgcat ccagaaggca accaccacca aataggcaac aaaagcgttg
     3301 tcgggtagcg ccccgctgtc agcggggcgc ttttttt
//
LOCUS       DBNGZD010000058         5572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_841383, whole genome shotgun sequence.
ACCESSION   DBNGZD010000058 DBNGZD010000000
VERSION     DBNGZD010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_841383"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="AC16GM_01650"
     CDS             complement(<1..2260)
                     /locus_tag="AC16GM_01650"
                     /inference="COORDINATES: protein motif:HMM:NF046853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HIR2922412.1"
                     /translation="MKRFFKGSTALGTDLFSRLAIFVPVLFLGGFFSVTSALAQEQKP
                     SRSVLNELLARGLDPRWSRIYPDTFSSQVLPPPVVREVPAPAGPKQVRLQNRTAVVSP
                     NMRMHPSVTTSQSEMSIWTHPTNPDIVLAGSNAAHVSVSVVSQGFYYTTNGGTSWTGR
                     DTLPTHTNFSLFMSDPAVGIDLDGNLFFNALLYGATGDLICARSTNNGTSWTQVTIPN
                     ATNGEDKNHFAVDVNPGSPFLNNLYTAYTDFDLSPSPVMFSRSTNRGVSFSSVIPISG
                     GIGAQFAQGVNLAVGPNGEVYATWTGYDVFPPAPTSRLGFNKSTNGGVTWGTATSIRS
                     VVDIRGTLSKGGNSIRVNSFPSMAVDRSSGPRRGWIYIVYPEKNPVSPDIFLVRSTDG
                     GSTWSTARKVNQDNSGKDQWFSWVSVDPVTGILYVAYYDSRNFAANDSAQVFLTASLD
                     GGDTFADLLVSDVPFLPTPIPGLAAGYMGDYIGVSSVNGVVWPCWNDNRTGIHQAWTS
                     RIVFITVGSPPQISVSPETLDYGNVFLAYPETLSISIRNLGFPDTLFVTNIQSDNSDF
                     TPELTSLALPGGGSRAVKVAFNPSTAGLVNATLSIASNDTANPTVTVALRANALIPPD
                     ISVSPDSLEADLLTGASAVETVTVTNSGGFRQSASLFSIFLRGLVFIDDVIGCNGNFP
                     LKLRSQPGELFYRDRLTGRQHFGRPDRLVCNEVDLRGRPAGSCHSNVKVQIAPAGVRN
                     RHRLHRRGAGQKI"
     gene            3446..4342
                     /locus_tag="AC16GM_01655"
     CDS             3446..4342
                     /locus_tag="AC16GM_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR2922413.1"
                     /translation="MSNFEIVARQDFSDVTYLLEIRHPLLAKAAQPGQFVIVMSQARG
                     ERIPLTIADFDREKGTITLVVQAVGKTTREMQQTCQPGTALFAVVGPMGLPSPIGKAK
                     KVLCVGGGLGVAPVFPQARAFRQAGAYVIGVIGFRNKNLIFWDDKFRSCCNEFILCTD
                     DGSAGIKGTVVEGIRLALEKHPDIDLCVAIGPPVMMKGCAEATRPRKIKTVVSLNPLM
                     VDGTGMCGGCRVKVGSAVKFACVEGPDFDAHQVDFDDLVSRLRRFAEEEKVALERWSE
                     TCRLNQPADALLPLSSTDHKSY"
     gene            4346..>5572
                     /gene="gltA"
                     /locus_tag="AC16GM_01660"
     CDS             4346..>5572
                     /gene="gltA"
                     /locus_tag="AC16GM_01660"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR2922414.1"
                     /translation="MAKKKTIRTIPQKRTPSREQDARERVKNFAEVSGGYRLEDVLNE
                     AERCLLCPEQPCIAGCPVGINIPGFIQKITEKDFRGAYDIITATNLLPAVCGRVCPQE
                     TQCEEVCIVGETLEPVAIGRLERWVGDTAIAEGWTNVPYIEPSGFRIGIVGSGPAGMA
                     CAADMAKAGCEVTVYEAFHEPGGVLRYGIPDFRLPNEVINAEIRNLAKLGVKFECNTL
                     VGRLFTIEEMIDELGYDAVFIGTGAGYPALLGIPGDSLNGVLSANELLTRCNLMRAKE
                     FPNYDTPLHLGRRVAVIGAGNTAMDALRVCLRLGAEKVYCIYRRSKVECPARAEEVHH
                     AEEEGVEFHWLTNPIEILDDGQGGVQGMRCIRMELGEPDESGRRRPVPVAGSEHEFET
                     DMVVFAIGTNANPIIGQ"
BASE COUNT         1235 a         1559 c         1675 g         1103 t
ORIGIN      
        1 cgatcttttg accggcgcct cggcggtgga gacggtgacg gttacgaact ccggcgggag
       61 cgatctggac tttgacattg ctgtggcagg acccggcggg gcggccgcga aggtcaactt
      121 cattgcaaac caagcgatcc ggtcggccaa agtgctggcg accggtaagc cggtcccgat
      181 agaacagttc acccggctga gatctcagtt tcaacgggaa atttccgttg cagccgataa
      241 cgtcgtcgat aaaaaccaac cctctcagaa aaatcgagaa cagagaggcg ctctggcgaa
      301 agccgccgga gttcgtaacc gtcaccgtct ccaccgccga ggcgccggtc aaaagatcgg
      361 cctccagtga atcaggtgag acggaaatgt ctggcggaat caacgcgttg gcccgcaggg
      421 ccaccgtcac ggttggatta gcggtatcgt tgctggcgat ggagagcgtg gcgttgacga
      481 ggcccgcagt cgagggattg aaggcgactt tcaccgcgcg actccccccg ccggggagag
      541 ccagagaggt caactccgga gtgaagtcgg aattgtcgga ctggatattc gttacaaaaa
      601 gcgtatcggg aaagcccagg ttacggatcg atatgctcaa ggtttccggg taggccagga
      661 agacattccc gtagtcaagc gtctcgggcg aaacgctgat ctgcggggga ctgccgacgg
      721 tgatgaaaac gatgcgggag gtccaggcct ggtggatgcc cgttcggttg tcgttccaac
      781 agggccagac caccccgttg actgaggaaa ctccaatgta atcccccatg taaccggccg
      841 ccaggccggg aatcggggtc gggaggaagg gcacgtcgct gaccaggagg tcagcaaacg
      901 tatcgccgcc atccaaagag gcggtgagaa agacctgcgc cgagtcgttg gccgcgaaat
      961 tgcgactgtc atagtaggcc acgtagagaa tacccgtcac cggatcgacg gaaacccagg
     1021 aaaaccactg gtctttgccg ctattatcct gattcacttt ccgcgcggtg ctccaggtgc
     1081 tgccgccgtc ggtggagcgc accaggaaga tatccggaga gaccggattt ttctccgggt
     1141 agacgatgta aatccaccct cgccgggggc cactgctgcg gtcgaccgcc atggagggaa
     1201 agctgttgac ccggatggaa ttgccccctt tgctcagcgt gccgcggata tccaccacgg
     1261 agcgaatact ggttgccgtt ccccaggtga ctcccccatt ggtcgatttg ttaaacccca
     1321 ggcggctggt gggcgccggc gggaagacat catagccggt ccaggtggca tagacctccc
     1381 cgttcggccc aaccgctaaa ttgactccct gggcaaactg cgcgccaatg cccccactga
     1441 tcggaatcac actggaaaaa cttacccctc tattggtcga gcgagaaaac atgactgggc
     1501 tggggctgag atcaaagtcg gtgtaggctg tgtagagatt attcaggaac gggcttcccg
     1561 ggttgacgtc caccgcgaag tggtttttgt cctcgccatt ggtggcattg ggtatcgtga
     1621 cctgcgtcca ggacgtgcca ttgttcgtgg agcgggcaca gatcagatcc ccggtggctc
     1681 cgtagagcag ggcattgaaa aataagttac catccagatc gataccgacg gccgggtcgg
     1741 acatgaacag gctgaaattc gtgtgcgtcg gcaacgtatc cctgccggtc cagctcgtcc
     1801 caccgttggt ggtgtaatag aatccctggc tcaccacgct gaccgaaacg tgtgccgcat
     1861 ttgacccggc caaaacaata tcggggttgg tgggatgcgt ccaaatagac atttcgcttt
     1921 gggaggtcgt cacactcggg tgcatacgca tgttgggtga aaccactgct gtccgatttt
     1981 gcagccgcac ctgtttcggc ccggccggag cgggaacctc ccggaccact ggggggggca
     2041 ggacttgaga cgagaacgtg tcggggtaaa ttcttgacca gcgcggatcc aggccgcgcg
     2101 cgagcaactc gttgagaacg gaacgggagg gtttctgttc ctgggccaaa gcggaagtta
     2161 ccgaaaagaa cccgcccaag aacaggactg gaacgaaaat agccagccgg gaaaaaaggt
     2221 cggtccccag agcagttgag cccttaaaga accgtttcat cggctgagtg aatgttgaat
     2281 tggtagagcc gctcaaaacc cgtgacctaa aagacatact gcctcccttc actttttctt
     2341 ttagtcgggt cccccccgac agagatagca tacctccctc ttttattttg tcaagtcttt
     2401 tcaagtattt tttcaaaaaa aagtgggcta aaattcaaaa aagggctgaa gaaaagagtc
     2461 ttggataacc ttagatacca caattgatta ctcgttcctt ttcctaaccg gtcagggaca
     2521 cgagctctct caggacatgc tacctatgca tttcaagctg cccggctccg aataactcga
     2581 aggtactaac gagcgcggct gctactcaga actttttgtc cttccgcagt tgcggtgccc
     2641 gagagaaagt gaccgaggac ttccgttagg gacaaaaaag caacatcccg tacgatgagc
     2701 atcaggaaga aaattagccc caccagtctc cgaccgactt gttgaatatg caatttccct
     2761 ggttgtaaaa aatggccgtt tcgccccagc gaagccagag gatgcaacga gatgcttgaa
     2821 cgtcgttata acccacgacc tgacccggcc gaggtctctg ccggtcgcaa caggaatatt
     2881 cttgaaaacc agaggtgatc aaaagcagga ccttaaagcc gaggcgtttt caaaaccggg
     2941 aagccccaac ccagcggttg tcagcggtcc ctcggtaaaa cgaccttgcg caatcgacaa
     3001 ggtatgttat ctttctaccg gaatggtcaa agagattctg actgcgtatt ctgaaatctc
     3061 caagttgcgt atccgacggc aggcaacctt gtgcgtaagg tcgtctccga aaagaagtac
     3121 agtggctttt cacggtaacc cagaatttgc tcgatttatg caagcgcttt gtcctctccc
     3181 caagtcgcgg ctcggcgcat ttcaaagatt tttcgaccac tggtccccag agcctggacg
     3241 acatagagct tgttacccgg accacaagct gcctcagaac taacggcagg cctttgaatt
     3301 gacgataatg aacgacaagg gaaaaaaccg gcctctcacc ctccctccac tccatctcct
     3361 cgacttggag cgccggcaaa ctgatcgtgg acggtgtttt gcccgaacgt gtctggatat
     3421 agaatattaa ggtacagata ctgaaatgtc caattttgaa attgtggcac gccaggattt
     3481 ctctgacgta acgtatctat tggagatccg tcatccgctg ctggccaagg ccgcgcagcc
     3541 gggccagttc gtcatcgtca tgtcgcaggc ccggggcgag cgcatcccgc tgacaattgc
     3601 cgacttcgac agggagaagg ggactatcac gctggtcgtc caggcggtcg ggaagacaac
     3661 cagagagatg caacagacct gccagccggg gacagcgctt ttcgccgtcg tcggtcccat
     3721 gggtctgccc agccctatcg gcaaggcgaa gaaggtgctg tgcgtcggcg gcggcctggg
     3781 ggtggcgccg gtctttccgc aggcgcgggc cttccgtcag gccggcgcgt atgtgatcgg
     3841 cgtcattggg ttccgcaaca aaaacctgat cttctgggac gacaagttcc ggtcctgctg
     3901 taacgagttc atcctgtgca ccgatgacgg ttcggcaggg atcaagggaa cggtcgtcga
     3961 gggcatccgg ctggctctcg aaaagcaccc cgacatcgac ctgtgcgtcg ccattggccc
     4021 gccggttatg atgaaggggt gcgccgaggc gacgcggccc cgcaaaatca agactgtggt
     4081 aagtctcaac ccgctgatgg ttgatggcac cgggatgtgc ggcgggtgcc gggtgaaggt
     4141 gggtagtgca gtgaagttcg cgtgtgtcga agggccggac tttgatgccc atcaggtcga
     4201 ttttgacgat ctcgtgtccc gcctgcggcg ctttgccgag gaggaaaaag tggcgctgga
     4261 gcgctggtcg gaaacctgcc gtctgaacca gccggccgac gccctgttgc cgctttcatc
     4321 caccgatcac aaaagttact agaccatggc caagaaaaag accatacgga cgatcccgca
     4381 aaagcggacc ccctcgcgcg agcaggacgc gcgcgaacgg gtcaagaact ttgccgaggt
     4441 gtcgggcggc tatcgtctcg aagatgtgct gaacgaggcc gagcgttgcc tgctttgtcc
     4501 ggagcagccc tgtatcgccg gctgcccggt cggcatcaat attcccggtt tcattcagaa
     4561 aatcaccgag aaggacttcc gcggcgccta cgacatcatc accgccacca atcttttgcc
     4621 cgcggtgtgc ggccgggtct gcccgcagga gacgcaatgc gaggaggtct gcatcgtggg
     4681 cgagacgctg gagcccgtgg cgatcggccg gcttgaacgc tgggtcggtg atactgccat
     4741 cgcggaaggc tggaccaatg ttccctatat cgaacccagc ggcttccgga ttggaattgt
     4801 tggctccggg cccgccggca tggcctgcgc cgccgacatg gccaaggcgg ggtgcgaggt
     4861 cacggtttac gaagctttcc acgagcccgg gggcgtcttg aggtacggca ttcccgattt
     4921 tcgccttccc aacgaagtga tcaacgccga gatcagaaat ctcgccaaac tgggcgtgaa
     4981 gtttgaatgc aacacgctgg tcgggcgcct gttcacgatc gaagagatga tagacgagct
     5041 cggctacgac gcggtcttca tcggaacggg cgcaggttac cccgcactgc tgggaatccc
     5101 cggcgactct ttgaacggcg tgctttcggc caatgagcta ttgactcgct gcaacctcat
     5161 gcgtgcaaag gagttcccga attacgacac gcccttgcat ctcggacggc gcgtggcggt
     5221 catcggtgcc ggcaacactg cgatggacgc cctgcgggtc tgcctcaggt tgggggcgga
     5281 aaaggtttac tgcatctacc gccgcagcaa ggtggaatgc ccggcacggg ccgaagaggt
     5341 ccatcacgcc gaggaggaag gcgtcgaatt ccactggctg accaacccga ttgagatctt
     5401 agacgacggc cagggcggtg tccagggtat gcgctgcatc cgcatggagc tcggcgagcc
     5461 ggatgagtcc gggcgccgcc gcccggttcc cgtcgcgggc agcgagcatg agttcgaaac
     5521 cgacatggtg gtgttcgcaa tcgggacgaa cgcgaacccg atcataggac ag
//
LOCUS       DBNGZD010000059         3619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_355447, whole genome shotgun sequence.
ACCESSION   DBNGZD010000059 DBNGZD010000000
VERSION     DBNGZD010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_355447"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="AC16GM_01665"
     CDS             <1..638
                     /locus_tag="AC16GM_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR2922415.1"
                     /translation="ASLGTAGFLRLAGQPKEAELKIDGKRQSLAAEPLKVLPGRRTIE
                     VLQNGQVIYTGLVDIRTGETITVDTSLKRIMPGLLALQVVPPDALIFIDGVAVSLVNG
                     RAELPAGGHTLKATKIGYQPYDGKFTLSSGEQTSLTINLEPASQPATPALALNATPKP
                     SKPLFKKPIFWGILGAAVIGGVVVAAGGGGGRGTDGGTTALPPPPPPPGKR"
     gene            681..3599
                     /locus_tag="AC16GM_01670"
     CDS             681..3599
                     /locus_tag="AC16GM_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2922416.1"
                     /translation="MQQWKRFWLTCTVLTVWGWLASNALAEPQKPLWGQDYLAYDGRV
                     AAFAADYTTDGSIYAVVVLAASPDSVVLLKSDNGGACWSEIGGRLVASGDSISEVRLA
                     TIEYGTVKGLLIFSLTRLGGGVLRATRFSTAGVFNAQSTVLTGILGDTLERFSVAPNL
                     ERGDTLVLAFGTKKGSVGVTRSVNAGATWGAYRLLVVGFTGLPDLAYGAKGAYFLACY
                     NSSTGEIQTFVSRDYGLTWTGPAAVARDTLRLALDLQIMAEHDANVDSTALWIFTNRL
                     ETDALLCGAGISFCFQLDLFFDASRDAGGKWSADSNLTSTPCCCNAGATVGYHEILPR
                     LAVSKVKPTRVSDLTYILVGGACSGVSAVEHLSSTVDAPDIWTVPQAVNVNGINEVIP
                     AQIVYSNAAARNGPAIFYAGKSGQGLYLQAGWLAATAGPVVCTRVAATASVGQGFDVV
                     ATVTDTAGLAQVKLFYRIIGRNAFGSIPMTGSSNTYIATIPAESVTVRGLEYYVEATN
                     NNSQTTLQPASAPSVPAKVAAVITNLVSPVFLPAAAYRMVAFPFLVYDSAATGTLFDD
                     LGSYNNTLWRLFRWNPTSATPAYDEYPALPPFAAGKGFWLISRNPAQFDASGISTVSD
                     NTNHVDVSLDRGWNQISSPYFWNVKIDSVRVDTGGAFTASFQQAVNRGILDSTMWELN
                     LSGSGYLRTHDLSPWSGAWIFNQRNYPIRLRVPAREQAGLGKIALAAPPPGWQVSVAL
                     SAGQYEDRENLFGVHPDARPGRDKFDLGEPPPIGPLVSLAFENSGAVLAADIRPPFTD
                     GGRWEMVVKNPQVNSPVRLEFAGVEGISPNFAAILFDPISGTKTDLRQTSVFTFEFTP
                     EQSERRFMILVGTPKFVATTSLVPYRFALEQNYPNPFNPATAIAYSLPVQQQVSLLVF
                     NILGQKVRTLVSGLEQAGPHQVTWNGKDDAGTEVASGIYFYRLAAGDERETKKMLLVR
                     "
BASE COUNT          681 a         1092 c         1132 g          714 t
ORIGIN      
        1 tggccagtct gggcacagcc gggttcctgc gcctggcggg tcagccgaaa gaggcggagc
       61 tcaaaatcga cggtaaaagg caaagtctcg cggctgaacc tttgaaagtc ctgccgggga
      121 ggcggaccat cgaggtcttg cagaacggcc aggtgatcta caccggtctg gtcgacatcc
      181 gaaccggtga gacaatcacc gttgatacca gtctcaaaag gatcatgccg ggattgctgg
      241 cgctgcaggt tgttcccccc gacgccctga ttttcatcga tggtgtggcg gtctccctgg
      301 tcaacggccg ggccgaactc cctgctggcg gacacacgct gaaagcaacc aagattgggt
      361 accagccgta tgacggaaaa ttcaccctga gctcgggcga gcaaaccagt ttaacaataa
      421 atctcgagcc ggccagccag ccggccactc ctgctttggc gctcaacgca acgccgaaac
      481 cttccaagcc acttttcaaa aagcctatct tctggggcat tctgggggcc gcggtgatcg
      541 gcggcgtggt ggtcgccgcg ggtggcggag gaggccgggg gaccgatggg ggtacaactg
      601 ctttaccgcc gccgccaccg ccgccgggga agcgctaagt ccggacttct gcaatctgtt
      661 ggtaaaaggt ggagggaacc atgcagcaat ggaaacgatt ttggctcacg tgcacagtat
      721 tgacggtctg gggttggctc gcgagcaacg cactggcgga accccaaaaa ccgctttggg
      781 gacaggacta cctggcctac gacggccgcg ttgccgcttt tgccgccgat tacaccaccg
      841 acggttcaat atatgcggtc gtggtgctgg cggcttctcc tgattccgtt gtcctcttga
      901 aatcggacaa cggcggcgcc tgttggtcgg agatcggcgg gcggctggtg gcgtcgggcg
      961 attcgatcag cgaggtacgc ctggccacca tcgagtacgg caccgtgaaa ggacttttga
     1021 tcttcagtct gacgcggctg gggggcggag tgctgcgcgc cacccgtttt tccaccgcgg
     1081 gagtattcaa cgcgcagagc accgtcctga cgggaattct cggagatacc ctggagcgtt
     1141 tctcggtggc gccgaatctg gaacgggggg atacgctggt cttggcattt gggacgaaga
     1201 aggggagcgt gggggtgacg cgcagcgtca acgcaggagc gacctggggc gcctaccgcc
     1261 tgctcgtcgt cggcttcacc ggcctcccgg atctcgccta tggcgccaaa ggcgcctact
     1321 ttctcgcctg ttacaattcg tcaaccggcg aaattcaaac gtttgtctcc cgagattacg
     1381 gtttgacctg gaccggaccg gcagcggtgg cccgcgatac cctgcggctc gccctggatc
     1441 tgcaaattat ggccgagcac gacgcgaacg tcgattccac cgccctgtgg atttttacca
     1501 accggctgga aaccgacgcg ctcctctgcg gggccggcat aagtttctgt ttccagttgg
     1561 atttgttctt cgatgccagc cgcgacgccg gcggcaaatg gagcgcagat tccaatctga
     1621 ccagtactcc ctgctgctgc aacgccggcg cgacggtagg gtaccacgaa atcctcccac
     1681 ggctggccgt ctccaaagtg aagccgaccc gggtgagcga cctgacctac atcctggtgg
     1741 ggggcgcgtg cagcggggtt tcggcagtag agcacctctc ctccaccgtg gacgcacccg
     1801 atatctggac ggtgccccag gcggtgaacg tcaatggcat caatgaagtg attcccgcgc
     1861 agattgtcta ctccaacgca gcagcgcgga acgggccggc aatcttttat gccgggaaaa
     1921 gcggccaggg actctacctg caagccgggt ggcttgctgc caccgcgggg ccggtagttt
     1981 gcacccgggt cgcggccacc gccagcgtgg ggcagggttt cgacgtggtg gcgacggtaa
     2041 ccgacacagc ggggctggca caggtaaaac tcttctaccg gattattggg cgcaatgcct
     2101 ttggcagcat tcccatgacc gggtcgagca acacgtacat tgcgaccatt ccggcggaat
     2161 cggtgacggt gcgcggactg gaatattacg tggaagcgac caacaacaac agccagacca
     2221 ccctgcagcc agccagcgcc cccagcgtcc cggctaaagt ggcggcggtc attaccaatc
     2281 tggtgagccc cgtcttcctg cccgcggcgg cctaccggat ggtggcgttc cccttcttag
     2341 tttacgacag cgccgccacc gggactttgt tcgacgatct cggcagttac aataacaccc
     2401 tctggcggct tttccgctgg aatcccacct ccgccacccc ggcgtacgac gagtatccgg
     2461 ctctgccgcc ttttgcggcc gggaaagggt tctggctcat cagccggaat ccggcccagt
     2521 ttgacgcctc aggcatttcc acggtttcgg acaataccaa ccacgtcgat gtctccctcg
     2581 accgcggctg gaaccagatc agttcgcctt acttctggaa tgtcaagatc gactcggtgc
     2641 gggtcgatac cggcggagcg tttacggcca gttttcagca ggcggtgaac cgggggattc
     2701 tggacagcac catgtgggaa ttgaatctct ccggctccgg ttacctgcgg acgcacgacc
     2761 tttcgccctg gtccggcgcc tggattttca accagcgcaa ctatccgatt cgcctgcgcg
     2821 tgccggcacg ggagcaggcc ggtttgggga agatcgccct cgctgctccc cctccgggct
     2881 ggcaggtaag cgtagcgctc tccgccgggc aatacgaaga tcgggagaat ctcttcggcg
     2941 tgcacccgga tgccagaccg gggcgggaca agttcgactt gggcgagccg ccccccatcg
     3001 gccctctggt ttctttagcc ttcgaaaatt ccggggccgt cctggcagcc gacattcgtc
     3061 caccctttac cgacggcggg cgctgggaga tggtggtcaa aaatccgcag gtgaactctc
     3121 cggtgcgtct ggaatttgcg ggcgtggagg ggatatcccc caacttcgcg gccattcttt
     3181 ttgatcccat ctccgggacg aagaccgatt tgcgtcagac gtcggttttc acgtttgagt
     3241 tcactccgga acagagcgag cgccggttca tgattttggt cggtacgccg aagtttgtgg
     3301 ctacgacgtc actcgtcccg tatcgctttg cgctggaaca gaactacccg aaccccttca
     3361 atccggcaac ggcgattgct tactcgctgc cggtacagca gcaggtctcg cttctggtgt
     3421 tcaacatcct agggcagaaa gtgcggacgc tggtttccgg tctcgagcag gccgggccgc
     3481 accaggtgac ttggaacgga aaggacgacg ccgggacgga agtcgcgagc gggatctact
     3541 tctaccggct ggcggcaggg gacgagcgcg agacgaagaa gatgcttttg gtacgttgag
     3601 aagattgaag tagtcgggc
//
LOCUS       DBNGZD010000060         5955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1536398, whole genome shotgun sequence.
ACCESSION   DBNGZD010000060 DBNGZD010000000
VERSION     DBNGZD010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1536398"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="AC16GM_01675"
     CDS             complement(<1..300)
                     /locus_tag="AC16GM_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR2922417.1"
                     /translation="MRIYAGNLGYDITEDDLKQAFGAFGSVAAVEMMVDKFSNQPRGF
                     AFVEMANAQEAQAAIEGLNGKELKGQTITVNEARPRTERFSGGGGGYGGGGGYGGG"
     gene            387..1061
                     /gene="tenA"
                     /locus_tag="AC16GM_01680"
     CDS             387..1061
                     /gene="tenA"
                     /locus_tag="AC16GM_01680"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015057.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HIR2922418.1"
                     /translation="MDFSLYLRRQADPMWESSFAHPFVQGIGKGTLPEEKFIFFLEQD
                     YVYLIEYCRFFGLAVAKGETLADMQKYAEVLHALLHFEMDLHRSVCAEFGISSQKLEQ
                     VKPAPDNLGYTSYLLKTAYEGDAGDILAALLPCLWGYVEIGQRLKAQGLPAHKHYRRW
                     IETYASPEFAEIADWCKARLNTFASGQKPEKLKRMEEIFLTTSRWEYLFWEMAWSGKQ
                     DFSRNA"
     gene            1054..1953
                     /locus_tag="AC16GM_01685"
     CDS             1054..1953
                     /locus_tag="AC16GM_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922419.1"
                     /translation="MHKQKLISILGGGFSLLIPELLEKAVVPYQRRGATKKLHKVSVL
                     EGCYSAAAILLACLMIESYRMRVVYFWRRRNVDRRKEIEKSVAQTLTDCLVEKEPKFP
                     KVAFCTVLNEIFVLRDVIAHNHLYEIFERYDSDWNLRNVRHRRLLGFFDRARFRSHVN
                     LSNRKTKILKCNVQPLRIGYEDALKVLIVCDTLIGVFERSYGRGLMPLILNYKAGNTW
                     TQSLGELISLLFEKLTAKDRYRVAKEVLLPLSVRFSTYLPNAGDWFLKNQCPGCNFFG
                     FRRFNRDIRCYKCNFSVKFVSLR"
     gene            2332..4071
                     /locus_tag="AC16GM_01690"
     CDS             2332..4071
                     /locus_tag="AC16GM_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR2922420.1"
                     /translation="MHILGISAFYHDAAACLLKDGEVVAAAQEERFSRIKHDFNFPIK
                     AIQYCLKEAGITSKDLSYVGFYEKPLLKFERILYQYFSTFPRSFNSFRQALPIWLKEK
                     LWIPGLIRKKLDYEGEILFIEHHLSHAASTFLVSPFKEAAILTIDGVGEWATATYGIG
                     RDNDITIFKEIRFPHSLGLLYSSFTYYLGFRVNSAEYKVMGLAPYGEPKYVDLIKREI
                     IDIKEDGSFVLNQDYFAYTYGLTMTNGKFDHLFDGPPRESDDPLEQRHKDLAASVQKI
                     TEEVMLKMANHLHREAKTKNLCLAGGVALNCVANGRILRETPFAELFNQPAASYAGGA
                     VGVATYIWHTLLKKERRWTWPHAFWGPEYSDEEIRSFLESQKVPFHRYERRKLLDHTA
                     QLIDEQKVVGWFQGRMEFGPRALGSRSILADARNPRNRDVVNLKIKFRESFRPFAPAV
                     LAEKASDYFELSCPSPYMLLTAQVRPDRRTIPAVTHVDGSARVQTVAREDNSRFYDLL
                     AAFAQRTGCPVLINTSFNVRGEPIVSSPEEAFRGFVNTDMDVLVIGSFVLLKVEMPPG
                     LRERGWYKKFQPD"
     gene            4097..4276
                     /locus_tag="AC16GM_01695"
     CDS             4097..4276
                     /locus_tag="AC16GM_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HIR2922421.1"
                     /translation="MVKVGGVKEKVSLARELWAFMRTRKKWWLGPILFILLLMAALII
                     FAETSAIAPFIYTLF"
     gene            4289..5353
                     /locus_tag="AC16GM_01700"
     CDS             4289..5353
                     /locus_tag="AC16GM_01700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR2922422.1"
                     /translation="MPRFVKNLLLAIASVLIFFGSIEVVLSLLHVEEKFQKRTFLLEP
                     ERDYPQFFAKDRNLFWKFRPNQTIQSHFFVEGEYRINSHGFRDREFSVAKPPGTFRIV
                     CLGNSCTFGWGVRQEETYPKVLEKILSDSLPGRKVEVINAGVTGYSSLQGLRLFEREI
                     LKLQPDLVTVCYGWNDHWPAVGGIPDTNQRLPAQWVLDLENFFDRLQTYRLLRRALGG
                     VSLNKQRYQDKSGATRLRATPEEVEANLRELAFQAKARGISVILVTPPSASPAWPWNE
                     IDYKRFTRIWQVHQQYTSAVRMTAQRWQIRLVDADFDFQRSNDYYDNPTNDYIHYNAK
                     GHARMGQLLYEALAPQIRQP"
     gene            5431..5503
                     /locus_tag="AC16GM_01705"
     tRNA            5431..5503
                     /locus_tag="AC16GM_01705"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5465..5467,aa:Glu,seq:ctc)
     gene            complement(5722..>5955)
                     /locus_tag="AC16GM_01710"
     CDS             complement(5722..>5955)
                     /locus_tag="AC16GM_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2922423.1"
                     /translation="TTTIRFALPEQASVQLVIYNVVGQKVRTLVDEELPAGEHSVVWD
                     GRDATGNVVASGVYLYKMVAGNHVLVQRMTLLK"
BASE COUNT         1327 a         1636 c         1588 g         1404 t
ORIGIN      
        1 cccgccgccg tagccgccgc caccaccgta gccaccgccg ccgccgctga accgctccgt
       61 gcggggccgg gcctcgttca ccgtgatggt ctgccctttc agttccttgc cgttcagccc
      121 ctcaatggcg gcctgcgcct cctgcgcatt ggccatctcc acgaaggcaa agccccgcgg
      181 ctggttgctg aacttgtcca ccatcatctc caccgccgcc actgatccga acgccccaaa
      241 ggcctgtttc agatcgtcct cggtgatgtc gtaccccaga ttccctgcgt agattctcac
      301 ttaccgctcc tttgttgttt ctaatatact cttttccgcg caatagccaa atggtatgtt
      361 accccccgcg ggccaaaacg aacgccatgg acttttccct ttacctccgc cggcaggccg
      421 atccgatgtg ggaatcatcc tttgcccacc ctttcgtgca gggcatcggc aaggggacgc
      481 tgccggaaga gaagttcatt ttctttttgg aacaggatta cgtttacctg atcgaatact
      541 gccgcttctt cggcctggcg gtggccaaag gggagacgtt ggccgatatg cagaagtacg
      601 ccgaggtgct ccacgccctc ctgcactttg agatggacct gcaccgttcc gtttgcgcgg
      661 aattcggaat atcctcgcag aagttagagc aggtcaagcc ggcgcccgac aatctcgggt
      721 acacgagtta cctcttgaaa acggcctacg agggggatgc gggcgatatt ttagccgcgc
      781 ttttgccctg cctgtggggg tatgtggaga tcggccagcg cctgaaagcg cagggcctgc
      841 ccgctcacaa gcactaccgc cggtggattg agacctatgc ctcgccggag ttcgccgaaa
      901 ttgccgactg gtgcaaggcc cggctcaata cgttcgcctc cggccagaag ccggagaaac
      961 tgaagcgaat ggaagagatt ttcctcacca cgagccggtg ggagtatctc ttctgggaga
     1021 tggcgtggag cggaaagcaa gacttcagta gaaatgcata aacaaaaatt aatctccatt
     1081 ctaggtggtg gcttttccct cttgattcct gaattgttag agaaggcggt tgtaccttat
     1141 caaaggcgtg gagcgacaaa gaaacttcat aaagtgtctg ttttggaagg atgttattcc
     1201 gccgctgcaa ttctgcttgc atgcctgatg attgaatctt accggatgcg cgtggtttat
     1261 ttttggaggc gtagaaacgt tgatcgcaga aaagaaattg aaaaatcggt tgctcagacc
     1321 ttgaccgatt gccttgtaga gaaagagccc aaatttccca aagtcgcatt ctgtactgtc
     1381 ctaaacgaga tttttgtttt gagagacgta attgcccaca accatctcta tgaaatattt
     1441 gagcgttatg attcagattg gaatttaaga aatgttagac atcgacgtct tttgggcttt
     1501 tttgatagag cgagatttag aagccatgta aatctctcga atcgaaaaac caaaatactc
     1561 aaatgtaatg tccaaccttt aaggatcggg tatgaagacg ctttgaaagt gctaatagtg
     1621 tgtgacacgc ttataggtgt gtttgaaagg tcctatggtc gtggactaat gcctttgatt
     1681 cttaactata aggcaggaaa tacatggacg cagtcacttg gagaactcat ttctctgctt
     1741 tttgaaaaac ttacagccaa agatcgttac cgagttgcga aggaggtcct actacctctt
     1801 tcagtcaggt tttctacata tctgcccaac gctggtgact ggtttttgaa aaatcagtgt
     1861 cctggatgta atttttttgg ttttcgacga tttaatcgag atattcgatg ttacaagtgt
     1921 aatttctcag tcaaatttgt ttccttaaga tgagggcctt cggcaatggg ggatagcgca
     1981 aaaaccactt ttagaaaggc ttggcaagtt tggaaatcct tcgcccaccg cctgggcaaa
     2041 atccagacca caatcattgt ctctctcttt tactttcttg tagttggcct cgtttggctg
     2101 gtctcttttc tcctccgcaa agaccttctg aacgtccgca ggcgcaatcg ccccaccttc
     2161 tgggtgtcca aagagccgat ggaggtgtcc tgggaaaggg cgaaaagaat gttttaatta
     2221 caagcagagc gggtgaatcc gcctgaggcg gattcacccc tacgtttctt aggaacattt
     2281 ccgcgaacga agggggtaca cgcagcgggg aaagggtgtt atatttttgc tatgcatatt
     2341 ctcggcattt cggcctttta tcacgatgcg gcggcctgcc tgctcaaaga cggtgaagtc
     2401 gtggcggcgg cgcaggagga aaggttttcc cgcatcaagc atgactttaa ctttcccatc
     2461 aaggccatcc agtactgtct gaaagaagcg gggataacaa gcaaagacct atcttacgtc
     2521 ggtttctacg aaaagcccct tttgaaattc gagcgcatcc tctatcaata cttctcgact
     2581 tttccccgct cgttcaactc tttccggcag gcgctgccca tctggctcaa ggaaaaactc
     2641 tggattcccg gcttgatccg caaaaaactc gactacgagg gcgaaattct tttcatcgag
     2701 caccacctgt cgcacgcggc gtccactttt ctggtttcgc ctttcaagga agcggcaatt
     2761 ctgaccatcg acggcgtggg agagtgggcg acggctacct acgggatcgg tcgggataat
     2821 gacatcacga ttttcaaaga aattcgcttc ccgcactctc tgggcctcct ctacagctct
     2881 ttcacatact atttaggttt tagagtgaac agcgcggagt ataaggtgat gggcctggcg
     2941 ccttacggtg agccgaagta cgtagacctc attaagcgcg agatcatcga catcaaagag
     3001 gacggctcgt tcgtgctgaa ccaggattac ttcgcctaca cctacggctt gaccatgacc
     3061 aacggcaaat tcgaccacct ttttgatggg ccgccccgtg aatccgacga tcctctggaa
     3121 caaaggcaca aagacctggc ggccagcgtc cagaaaatca ccgaagaggt gatgctcaag
     3181 atggccaacc acctccaccg cgaagcgaaa accaaaaacc tctgtctggc cggtggggtg
     3241 gcgctcaact gcgtggccaa cggacggatt ctgcgcgaga cgcctttcgc cgaactattc
     3301 aaccagcccg cggcaagcta cgccggcggc gcggtggggg tcgcaactta tatctggcat
     3361 accctcttga aaaaagaacg ccggtggacc tggccgcacg ctttctgggg gccggagtat
     3421 tccgacgaag aaatccgctc gtttttggaa tcgcagaagg taccctttca ccgctacgag
     3481 cggcggaagt tactcgatca cacggcgcaa ttgattgacg agcagaaagt ggtggggtgg
     3541 ttccaggggc ggatggaatt cggcccgcgc gccttgggca gccgctccat tctggccgat
     3601 gcgcgcaatc caaggaaccg ggacgtggtc aacctcaaaa tcaaattccg ggaatcgttc
     3661 cggccctttg cgccggcggt gttggccgag aaagcgtccg attacttcga actcagttgc
     3721 cccagcccct atatgctcct gaccgcgcag gtgcgccccg accggcgcac gattccggcg
     3781 gtgacgcacg tggacggctc ggcccgggtg cagacggttg cccgcgagga taattcgcgc
     3841 ttctacgacc ttctggcggc gttcgcgcag cgcaccgggt gtccggtgct catcaacacc
     3901 tcgttcaacg tgcgcgggga gccgatcgtc tcttcgccgg aggaggcctt ccggggtttt
     3961 gtcaataccg atatggatgt tctggtcatc ggttccttcg ttttgctcaa agttgagatg
     4021 ccgccgggct tgcgcgaacg cggctggtat aagaaattcc aacccgattg agattggtcg
     4081 gcagcgatga ggaggaatgg ttaaagtcgg cggtgtaaaa gaaaaagtat cgctggcgcg
     4141 tgagctgtgg gcctttatgc gcacgcgtaa gaagtggtgg ttggggccga ttcttttcat
     4201 tttgctcctg atggcggcgc tcattatctt cgccgagacc tcggccattg cgcccttcat
     4261 ctacaccttg ttttaacctc ccgccacgat gccgcgcttt gtcaaaaatc ttctcctggc
     4321 aattgcgagt gttctgattt tcttcggcag catcgaagtg gtgctcagtc tcctccacgt
     4381 cgaggagaag tttcaaaagc gcactttcct ccttgaaccc gaacgggact acccccagtt
     4441 ttttgccaaa gaccgcaacc tattctggaa attccgtccg aatcagacca tccagagcca
     4501 cttcttcgtc gagggagagt accgaatcaa ttcgcacggc ttcagggatc gggagttttc
     4561 ggtcgccaaa ccaccgggaa cgtttcgcat cgtctgcctc ggaaactctt gcaccttcgg
     4621 ctggggcgtg cggcaggagg agacttatcc caaggtactg gagaaaatac tctctgattc
     4681 gcttccgggg cggaaggttg aggtcatcaa tgcgggagtc accggttact cgtcgctcca
     4741 ggggttacgg ctctttgagc gggagattct caaactccag ccggacctcg tgaccgtctg
     4801 ctacggctgg aacgaccact ggccggcggt gggggggatt cccgatacca atcaacgtct
     4861 cccggctcag tgggtactgg atctcgagaa tttctttgat cggctgcaga cttaccgctt
     4921 gctcaggcgc gcactgggcg gcgtttccct gaataaacaa cgctatcagg ataagagcgg
     4981 agcgacgcgg ctgcgagcga cgccggagga ggtggaagcg aacctgcgcg agctggcgtt
     5041 ccaggcaaaa gcgcggggca tatcggtaat tctcgtaacg ccgccctccg cttcgcccgc
     5101 ctggccatgg aacgaaattg attacaaacg cttcactcgt atctggcagg tgcaccagca
     5161 gtacaccagt gccgtccgga tgaccgccca gcgctggcag attcggttag tagacgccga
     5221 ctttgatttc cagcggtcaa acgactacta cgataacccg accaacgact acatccacta
     5281 caacgccaaa ggccacgccc gcatggggca actgctctac gaggcgctgg ccccccaaat
     5341 caggcaaccg tgactattct taccaagagc agcctgttga cgcactacct ttttccctgt
     5401 aaactataga aaaatttgat tcttttttgc ggccccatcg tctagtggcc caggacgggt
     5461 ggttctcagc catcaaacca gggttcgact ccctgtgggg ctatacccga acattggaaa
     5521 tccgcccttg ccgcacaata gtcttgctgc ctgaactctt acggctccag ggtgactcaa
     5581 cgaattgagg gcttttcccg cccccagggc aggcagctct cggagtcatg aggaaaacgg
     5641 acaaaatcta tccctgaatc cgaagttcgg aaaaaacaaa aagccccgcc agaggcgggg
     5701 ctttttggac ttacgtggag tttactttaa gagggtcatg cgctgaacca aaacatggtt
     5761 cccggcgacc atcttgtaca ggtaaactcc cgaggctact acattgcccg tagcgtctcg
     5821 gccgtcccac accacggagt gttcgccggc gggcagttcc tcatctacca gggtccgtac
     5881 tttctgaccc acgacgttgt agatgacgag ttgcaccgac gcctgctccg gaagcgcgaa
     5941 gcgaatcgtg gtggt
//
LOCUS       DBNGZD010000061         2971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1605780, whole genome shotgun sequence.
ACCESSION   DBNGZD010000061 DBNGZD010000000
VERSION     DBNGZD010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1605780"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="AC16GM_01715"
     CDS             complement(<1..482)
                     /locus_tag="AC16GM_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR2922424.1"
                     /translation="MSVVAVDLQAFLAEKKKSIDSSLDSFLPKADQEPRRLHQAMRYA
                     VLDGGKRFRPILAIAAFEMAGGRGEVILPAACALELVHTFSLVHDDLPSLDNDLLRRS
                     QPSLHCAFDEATAILAGDALLALGFELLAQTGNAAVVAEVAQAIGSGGMIGGQMADLA
                     A"
     gene            complement(479..742)
                     /gene="xseB"
                     /locus_tag="AC16GM_01720"
     CDS             complement(479..742)
                     /gene="xseB"
                     /locus_tag="AC16GM_01720"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR2922425.1"
                     /translation="MPKKQAFEEALKRLEEIVEKLETGDVSLDESLAVFQEGIDLYRF
                     CHKRLTEAEKRVKVLIENVKGEFSLENFEPEGQGEAGADEEKS"
     gene            complement(829..2031)
                     /gene="xseA"
                     /locus_tag="AC16GM_01725"
     CDS             complement(829..2031)
                     /gene="xseA"
                     /locus_tag="AC16GM_01725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR2922426.1"
                     /translation="MLPKLFTVSELTRRIKENLEAGFPEVWVEGEISNYLLHTSGHRY
                     FSLKDESAQVRCVFWRRQARLLRFEPEDGMKVQIYGNLTVYEKQGQYQLNVLRILPKG
                     RGELELAFQQLKEKLFREGLFDEARKRPLPRFPEAIGIVTSPTGAAIRDLIEILTRRY
                     PPAQIILYPARVQGEDAGEEIEAGIKAFNRHGEADLIIVGRGGGSLEDLWAFNEERVA
                     RAIFHSRIPVVSAVGHEIDYTIADFVADLRAPTPSAAAELVVPEQGQLLAGLRGWLQS
                     LVNELERQVETRRERVVAIGKSYGFRRPLDFIAQRAQEVDEALRVLKFSASHRLDALR
                     GHLALLTGKLDALSPLAVFQRGYAMVLKLPERSLVREARVLQREDQVEVKVFKGRFQA
                     RVEETNVE"
     gene            complement(2241..>2971)
                     /locus_tag="AC16GM_01730"
     CDS             complement(2241..>2971)
                     /locus_tag="AC16GM_01730"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR2922427.1"
                     /translation="RACRSIGIHSEVKRLPKETTTAELLAVIDGLNRKPEIDGILIQA
                     PLPPQIDSRKIYVAVDPAKDVDGFHPQNLGEMLLGYSPFLPCTPLGVVELLVRSGHSA
                     EGKHVVILGRSNIVGKPLAALLLQKRSGCNATVTVVHTHTRNPLEQTRRADILVAAMG
                     NPEYVRGEMLREGCVVVDVGVNRVADPSQPKGYRVAGDVHFPSASEVAAAITPVPGGV
                     GPMTIAMLLSNTVQAAKQRRGKGL"
BASE COUNT          619 a          911 c          815 g          626 t
ORIGIN      
        1 gccgccaggt cggccatctg tccgcctatc atgccgccac tgccgatggc ttgcgctacc
       61 tcggccacca ctgcggcatt gccggtctga gccagaagtt cgaaccccaa agccagaagt
      121 gcgtcgccgg ctaaaatggc ggttgcctca tcaaaagcgc agtgcagtga tggctggctg
      181 cgccggagaa gatcattatc taaactgggt aaatcgtcgt ggactaaaga aaaggtgtgg
      241 accaattcga gagcgcaggc cgcgggcaaa atcacctctc ccctaccacc cgccatctcg
      301 aaagcggcga tggccagaat cggccggaag cgcttgccgc cgtcgaggac ggcgtagcgc
      361 atcgcctggt ggagcctcct gggttcctgg tccgctttcg ggagaaaaga atccagggag
      421 gagtcaatcg acttcttttt ttcagccaaa aacgcctgga gatcaaccgc caccacgctc
      481 acgacttttc ctcgtcagcc cctgcttctc cctgtccctc gggctcaaag ttttccaagc
      541 tgaattcgcc tttcacattt tcgattaaca ctttgactcg tttctcggct tcggtcaggc
      601 gcttgtggca gaaacggtag aggtcgatac cttcctggaa aacggccaag gattcgtcta
      661 aagatacgtc tcccgtttcc agtttctcaa cgatttcctc taatcgtttc agcgcttctt
      721 caaaggcttg ctttttgggc atagtgtccg gagtctcttc ggatgcaggt tacggtgccg
      781 gggtagtcgg gctcgtctcc gagcccgacg ggttagctca gggtacactc actcgacatt
      841 tgtttcttcc acccgcgctt gaaatcttcc tttgaaaacc ttgacctcca cctggtcctc
      901 ccgctgcaaa acgcgcgctt ctcgaacaag cgaccgctcc ggcagtttca gcaccatcgc
      961 gtagccgcgc tggaacaccg ccagaggcga gagcgcgtcc aatttgcctg tcaacagcgc
     1021 cagatgaccc cgtaaggcat ccaaccggtg ggaagcggaa aacttgagca cccgcagcgc
     1081 ttcatccacc tcctgcgcgc gctgggcaat gaaatccagc gggcgtcgga aaccgtacga
     1141 cttgccgatg gcaaccactc gttcccggcg tgtttccacc tgacgttcca attcattcac
     1201 cagactttgg agccaccccc gcagtccggc caaaagttgt ccctgctccg gcaccactaa
     1261 ctccgccgca gccgagggcg tgggcgcccg cagatcggcc acaaaatcgg caatggtgta
     1321 gtcgatctcg tgccccacgg cggagaccac cgggattctg gagtgaaaaa tggcccgcgc
     1381 cactctttcc tcattgaagg cccacaggtc ttcgagcgaa cctccaccgc gacctacaat
     1441 gatgaggtcc gcctctccgt gacgattgaa cgctttgatg ccggcttcaa tttcttctcc
     1501 ggcatcttcc ccctgcaccc gggccgggta gagaatgatt tgcgccggcg gatagcgccg
     1561 cgtaagaatt tcaatgagat cccgtatggc tgcaccggtg ggagaggtca caatgccgat
     1621 cgcttcggga aaacggggca aaggccgctt gcgggcttca tcaaataagc cctcgcggaa
     1681 cagtttctcc ttcaactgct gaaaggccag ttccagttcg ccccggcctt tgggcaaaat
     1741 ccgcaggacg tttaactggt actggccctg tttttcgtag acggtcaaat tgccgtaaat
     1801 ttgcactttc atcccgtctt ccggttcaaa gcgcaagagc cgcgcctgcc ggcgccagaa
     1861 gacgcatctg acctgggcgc tttcgtcttt gagcgaaaag tagcggtgcc cggaggtgtg
     1921 caggaggtag ttggaaattt caccctctac ccaaacttcg ggaaagccgg cttcgaggtt
     1981 ctctttgatc cggcgcgtca gttcggagac cgtgaaaagt ttggggagca tacaggcccc
     2041 gcccctggcg ggttcttcat aagatacgct tacgtcaccg acaccgtcaa ccggaaaccg
     2101 tcaggaaaaa atcggtgaaa gcgctcaatc aaaaaatgaa ggaatctaca caagggtagt
     2161 cgggctcgtc tcggagcccg ctaccctttt tgaccgactc agagacgaac cggtctaccg
     2221 atagcaaaaa aaacctgcaa tcagagacct ttcccgcggc gctgtttggc agcctggacg
     2281 gtattggaaa ggagcatggc gatggtcatc ggccccacgc cgcccggcac cggagtgatg
     2341 gccgcggcta cttcggacgc tgaggggaaa tggacgtcgc ccgccacccg gtaccctttc
     2401 ggctggctgg gatccgcgac gcggttgacg ccgacatcca ccacgacgca cccttctctc
     2461 aacatctcac cccgcacgta ctccggattg cccatggcgg ccaccagaat gtcggcccgc
     2521 cgggtttgct ccagaggatt ccgggtatgc gtatggacta cggtcaccgt cgcgttgcag
     2581 cccgagcgtt tctgcagcaa gagtgcggcc aggggttttc ccacaatatt gctgcgcccc
     2641 aggatcacca cgtgtttccc ttcggcggaa tggccagatc gtaccagcag ctcgaccacc
     2701 cccagggggg tgcagggcag aaagggggaa tatcccaaga gcatttctcc caaattttgg
     2761 ggatgaaatc catcgacgtc tttcgccggg tcgaccgcca cgtaaatctt cctcgaatcg
     2821 atctgcgggg gtaaaggtgc ttgaatgagg atgccgtcaa tctccggttt tcggttgagg
     2881 ccgtcgatga ctgccaaaag ctctgcggtg gtcgtctctt taggcaaccg cttcacttcg
     2941 gaatgaatgc cgatggagcg gcaggctctg g
//
LOCUS       DBNGZD010000062         2796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1397651, whole genome shotgun sequence.
ACCESSION   DBNGZD010000062 DBNGZD010000000
VERSION     DBNGZD010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1397651"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="AC16GM_01735"
     rRNA            complement(<1..1417)
                     /locus_tag="AC16GM_01735"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1668..1740)
                     /locus_tag="AC16GM_01740"
     tRNA            complement(1668..1740)
                     /locus_tag="AC16GM_01740"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1705..1707),aa:Ala,seq:tgc)
     gene            complement(1883..>2796)
                     /locus_tag="AC16GM_01745"
     CDS             complement(1883..>2796)
                     /locus_tag="AC16GM_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922428.1"
                     /translation="GVFIGQQEEHFRGPLFPVDQPQTRFNLIYGKEMSKVTLGLNFNF
                     SGVKTESRVGNVPTGTLLQDKAGTFGFGAGLGVPLTETQDLQVAGHIELRNFSSDTGN
                     VTVRQDDGNWSLLFLGRDFIEYNDMVTLVPAGQVEIFNEKSRRVPGPQPDTAHEIKGT
                     NWQFGLGCNVQPNEKVLINSAVGVRGGSSQHKIDQNLTEAGTSKTSFFTIPFALLGMD
                     VEVKKGLDFRAGATKEIRSDKNNILNTKNTDTDFTYAIGAAVRLGDVTFDTRVANTFL
                     NNGPYLLSGQTTFGGMFPQVSFTYAWK"
BASE COUNT          509 a          881 c          727 g          679 t
ORIGIN      
        1 gtgaacgggt ttctcgcccg ttttatcgct actcgttccg gcataatcgc ttccgcttcg
       61 tccagcgccg cttgtcgcga caccttcact ctacggcgga atgctcccct accactccga
      121 tattgctatc ggagtccgca gcttcggtgg ctggcttagt cccgggaatt atcggcgcgg
      181 agccactcga ccagtgagca gttacgcact ctttaaatga tggctgcttc taagccaaca
      241 tcctggttgt ttgcgtacct ccacatcctt agtcactgag ccaacacttg gggaccttag
      301 ctggcggtct gggttgtttc ccttttggca atgaagctta tcccccactg cctgtctccc
      361 gccgaacgaa ctggcggcat tcggagtttg gttcgggtcg gtagcccggt aaggcccctt
      421 gccgatccag tgctctacct ccgccagtct ttgcggcgag gctatacctc aatatatttc
      481 ggggagaacc agctatcgcc aggtttgatt agcctttcac ccctagggac agttcatccc
      541 ctcgcttttc aacgcaagtg ggttcggtcc tccatcccgt gttaccggga tttcaacctg
      601 accatcccta gatcacctgg cttcgggtct tctgcctgct acctgttccg cttgcgcgga
      661 acgcgccctg ttcagactcg ctttcgctcc ggctacggcc gttttggcct taacctttgc
      721 agcagacaag aaactcgccg gatcattatt caataggcac gcggtcagct ctctcccgcc
      781 cgaaggcgga aaatcgcctc ccaccgcttg taagcacatg gtttcaggta cttttcactc
      841 cccgtcaggg gtgcttttca cctttccctc gcggtactgg ttcactatcg gtcacacgca
      901 cgtatgtagc cttatcccgt ggtcgggacg gattcacgcg ggattgcacg tgtcccgcga
      961 tacttgggaa ccgtggcaac aggagcgtcg gaagtttcgc ctaccggact gtcaccgtct
     1021 ctggtttgct tttccacgca acttcggcta ccgccgacgt ttgtaactcc tgcggccgga
     1081 ctgcacccgg ccggattcca cggccccgct accccggcgg cacaacgcgt gcaggcttct
     1141 gcgtgccggc cggtttgggc tcctccccgt tcgctcgccg ctactagggg aatcgcaatt
     1201 gctttctttt cctgcaggta ctgagatgtt tcacttccct gcgtttgcct cctcccctac
     1261 gggaggatcg cccggcatga cccgggcggg gttgccccat tcggagatct ccgcctcact
     1321 gtgtgcgtgc cactcagcgg agcttatcgc agcttaccac gtccttcgtc gccgtcgtgt
     1381 gccaaggcat ccaccatgtg ccctttggaa cttgatccaa agtgctcgga tctgtgcttt
     1441 tggcaacaga tgcttgccca aaaatctgca accgccctcc ccgtacgggg agaactgcca
     1501 cagacattac ccgcggtatg aacttgtcaa agaaccggtg ctgaaggttt ttcgccttca
     1561 gccggaatgt ttgcgacact ccatccctgc tccgtctata ggtacgtgat tgggcaggga
     1621 aatgctatta taagagccaa tccctctggt tgtcaactgc tttcttatgg agataaccgg
     1681 gatcgaaccg gtaacctcct ggttgcaaac caggcgctct cccaattgag ctatatcccc
     1741 aacatacttt cgcgaccaga gaccagcggt gcaacttttc gcctctggta tgcggtccaa
     1801 aactggcgcc gctcaagatg accggtggct ctgttcagcg acagagccac cggcaaaaac
     1861 cgtacgaacg aactgcgctg aactacttcc aggcgtaggt gaacgaaacc tgcgggaaca
     1921 tcccgccgaa ggtcgtctgg ccggagagca agtacggccc gttgttcaag aacgtattgg
     1981 ccacccgggt gtcgaaggtt acgtcaccca gccgcaccgc cgccccaatg gcgtaggtga
     2041 aatcggtgtc ggtgttcttg gtgttcagga tgttgttctt gtcgctccgg atctctttgg
     2101 tagcacccgc ccggaagtcc agccctttct tcacttccac atccatgccc agaagagcaa
     2161 acgggatggt gaagaagctg gttttgctgg tacctgcttc ggtgagattc tggtcaattt
     2221 tgtgctgaga ggagccgccg cggaccccca cggccgagtt gatcaggact ttttcgttcg
     2281 gctgtacgtt gcaccccaac ccgaactgcc agttcgtccc tttgatctcg tgagctgtat
     2341 cgggctgggg accgggcacc ctccggctct tctcgttgaa gatttccacc tgacccgccg
     2401 gcacgagcgt taccatgtcg ttgtattcaa tgaagtcacg tcccaggaac aggaggctcc
     2461 agttgccgtc gtcctgtctc acggtcacgt tccccgtgtc ggagctgaag ttccgcagtt
     2521 cgatgtgacc ggccacctgg agatcctgcg tttcggtcag cggaacgccc aacccggcac
     2581 cgaaaccgaa tgtccccgcc ttgtcctgaa gcagggtgcc cgtgggcaca ttgccaaccc
     2641 ggctctcggt ttttacaccc gagaaattga agttcaaccc gagcgtcacc ttcgacattt
     2701 ccttcccgta aatcaggttg aaccgggttt ggggttgatc caccggaaac agaggacccc
     2761 ggaagtgctc ctcctgctgt ccaatgaata ccccca
//
LOCUS       DBNGZD010000063         2298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_772997, whole genome shotgun sequence.
ACCESSION   DBNGZD010000063 DBNGZD010000000
VERSION     DBNGZD010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_772997"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="AC16GM_01750"
     CDS             <1..1137
                     /locus_tag="AC16GM_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR2922429.1"
                     /translation="EAGLVTILDAKGQQVSQAIAGGEEKKRLLLELESELLISLRKNF
                     GVRSVYLTLDRDGTHTLFSYPLRSNGEVVGAISGVVSGTRNLALEEEFIEALGHQLGI
                     AVGKKVILPTLVKTERPAIVQEFLDYSLDLKQVLTRLHEKLSPRVKYDLVGLVHPRDA
                     VWVDLYLLRRGAGEVLEGRLERIEKTLLEQVASRRTPVLTNRVGETGLHAQVRGLQYV
                     GLVPLVFENDVVGVVYLGAAGMLTQPDLEELRALALPLALAVKNALLYGELRAAYQTL
                     SERQKELLKDERKTAVLEAAVTISHEVNNPLTAVLGNTQLLLLKKEQFPPEVQEKLKV
                     IEESALRIREVTQKLMNIEEPVSTEYVEGVKMLDLEKSMRRKKE"
     gene            1322..>2298
                     /locus_tag="AC16GM_01755"
     CDS             1322..>2298
                     /locus_tag="AC16GM_01755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2922430.1"
                     /translation="MASPKVLVVDDETSLCQFMQIMLAKEGYDVTALSTGQEALSRLA
                     QKRFDVVIADLMMPEMNGLELLSKVREIDPSLAFVVMTAFASVDSAIEALKKGAADYI
                     TKPFKVDEIKIALARLLEQKRLSSENLSLKRQLKKESDFTHFIGAAPAIVAMKKMAEQ
                     VAATDATVLIHGESGTGKEIVARAIHQLSPRADKPFVTINCAALPETLLESELFGHTR
                     GAFTGAVKDKEGLFTVADGGTFFLDEVGETTPAIQVKLLRALEEKEITPVGATKPIQV
                     DVRLIAATNADLEADVKNGKFRTDLFYRLNVFAIAIPPLRERQEDIRLLA"
BASE COUNT          524 a          648 c          677 g          449 t
ORIGIN      
        1 gaagccgggc tggtgaccat tctggatgcg aaagggcagc aggtctctca ggccattgca
       61 ggaggggagg agaaaaaacg gttattgctg gaactggagt cagaactcct catctccctc
      121 cggaaaaatt tcggtgtgcg cagcgtctat ttgactttgg accgtgacgg gacgcacacg
      181 ctgttttcct atcctttgcg ttccaacggc gaagtggtcg gcgctatttc gggggtcgtc
      241 tcgggcacgc gcaatctggc gttagaagaa gaattcatcg aggccttggg gcaccagtta
      301 ggcatcgcgg tcggcaaaaa ggtcattctc cccacgctgg tgaaaaccga gcgcccggcc
      361 attgtccagg agttcctcga ttacagtctt gacttaaaac aggtattgac ccgcctgcac
      421 gagaaactgt cgccgcgggt gaagtacgat ctcgtcggtt tggtccaccc ccgtgacgcc
      481 gtctgggttg atttgtacct cttgcgcagg ggggcaggag aagtcctgga agggcggctg
      541 gagcgaatcg aaaagacgct ccttgagcaa gtggcctcgc ggcgcacacc ggtgttgacc
      601 aaccgcgtcg gcgaaaccgg tctgcacgcg caggtgcggg gattgcagta cgtcggcctc
      661 gtgcccttgg tctttgaaaa tgacgtcgtc ggcgtggtct acctcggtgc cgcgggaatg
      721 ctcacccagc cggatcttga agaactgcgg gcgcttgcgc tcccacttgc cttggccgtg
      781 aagaatgcgc tcctctacgg tgaactgcgt gctgcttacc agacgttgag cgaaagacaa
      841 aaagaattac taaaggacga acggaagacg gcggtgctcg aggcggcggt caccatcagc
      901 cacgaagtga ataatccgct caccgcggtc ctgggcaaca cgcagttgct tttgctcaag
      961 aaagagcaat tccctcccga ggttcaggag aaactcaaag tgattgaaga gagcgccctc
     1021 aggatccggg aagtgaccca gaagttgatg aatatcgaag agccggtctc gaccgagtac
     1081 gtggaagggg tgaagatgct ggatctggag aaatctatgc ggagaaaaaa ggagtgaaac
     1141 tcagaacacc gaacacagta tacggagcac cgggaaagca aaaatcgcgt tgaaaggcga
     1201 ttttttttcg gtagcacttc ccggagataa gacggcacaa aggtttactc cacttttgtt
     1261 ctcgcggctt ctcgcccacg cgcctgtagc cggtctcccg tctgccgata actccaatag
     1321 tatggcatcc cccaaagttc tggtggtgga cgacgaaacc tctctgtgcc agttcatgca
     1381 gatcatgctg gccaaggaag gttacgatgt gaccgccctt tccacggggc aggaagcgct
     1441 ctcccgcctg gcgcagaaac gcttcgatgt ggtcatcgcc gacctgatga tgccggagat
     1501 gaacggctta gagttgttgt caaaagtgag ggaaatcgat ccctccctgg cctttgtcgt
     1561 gatgaccgcc tttgcctcgg tggacagcgc catcgaggct ttgaagaagg gggccgccga
     1621 ctacatcacc aagccgttca aagtggatga aatcaagatc gccctggcgc gcctcctgga
     1681 gcagaagcgg ctctccagcg aaaacttaag cctcaagcgc cagttgaaga aagagtccga
     1741 ctttacccat ttcatcgggg ccgcaccggc gatcgtcgcc atgaaaaaga tggcggaaca
     1801 ggtggccgcc accgatgcca ccgtcttaat tcacggagag agcggcaccg ggaaagaaat
     1861 agtggcccgg gccattcacc agctctcgcc ccgcgccgac aagcccttcg tcaccatcaa
     1921 ctgtgcggct ctgcccgaga cgcttctgga aagcgaactc ttcggccaca cccggggcgc
     1981 attcaccggc gcggtcaaag ataaagaggg cctcttcacc gtggccgacg gcggcacctt
     2041 cttcttggac gaagtgggag agaccactcc ggcgattcag gtcaaactcc tgcgggcctt
     2101 agaggagaaa gagatcacgc cggtgggagc caccaagccg atccaggtcg acgtccgtct
     2161 gatcgccgcc accaatgcgg acctcgaagc cgacgtaaaa aacggcaagt tccggaccga
     2221 cctcttctac cggctcaacg tctttgccat cgccattcct cctttgcgcg agcgccagga
     2281 ggatatccga cttctggc
//
LOCUS       DBNGZD010000064         2269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_495319, whole genome shotgun sequence.
ACCESSION   DBNGZD010000064 DBNGZD010000000
VERSION     DBNGZD010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_495319"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1819)
                     /locus_tag="AC16GM_01760"
     CDS             complement(257..1819)
                     /locus_tag="AC16GM_01760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR2922431.1"
                     /translation="MREPFPRSVLSRLQLVFVSSRVVTIFGVIAWLVYSPHREVNAGL
                     VQQLLIAFGLHLLIFYWLAARNFVSLPALYRVTTFFDLAFITLLTWATGGLTSNFYLF
                     YLLAISFASFYFGMAFGLLAAGLSTVAYLGTNAVTFSDLFLGDLLLRILLFWFFATAV
                     GLIAKYLRGSERRLLRALDTLNERTTELEHSQVQIETIYETSRSLGEMHNMHEVLDEI
                     LNIARRILGYPLCSVLLLNAREDRLLLMAKLESGEKQIYPEPIVVSLDGIAGSVARSG
                     KAERVSDVRIDPRYIPGLPGARSEMVVPMISRGNVIGVLNAESEQMSAFFERDQKIFS
                     ILASSAALALENAVMHNKMEELTIVDELTGTFNFRYFTMKLTDELRRANRYSQPLSLV
                     MIDIDWFKKTNDTYGHEKGNQVLQGLAQVIKNCIRDVDILCRYGGEEFVVILPQTDRR
                     DALVIGERVRSQVATTGFGNGSISPMVHITVSVGVTSYPENGRNQDEIVAVVDQALYR
                     AKGSGRNLVCAI"
BASE COUNT          531 a          605 c          710 g          423 t
ORIGIN      
        1 gagggcttcg ggccggggta gccgaagaca aaaagttgcc ctacgaggtt ttcggtgccg
       61 ggcatcgggg ggaatataag agttaattca cagcagatga agcagattga acacaatttc
      121 ccaaatcgga ctccttaagt tcgcaggact gacccagcgc aatccgaaac cgcagctggc
      181 aatctggtga agaagttagc gcaagcgaac agggcagagg tccgatccca cccagccagc
      241 cagggtagga taattcttaa atggcacaga ccaagttgcg gccggagccc ttggcgcggt
      301 agagggcctg atcgaccacc gccacgattt cgtcctggtt gcggccgttc tccggatagg
      361 aggtgacgcc cacggagacg gtgatgtgga ccatggggga gatggagccg ttgccgaagc
      421 cggtggtggc cacctggctg cgcacccgtt cgccgatcac caaggcgtcg cggcggtcgg
      481 tctggggcag gatcaccacg aactcctcgc cgccgtagcg acaaaggata tcgacgtcgc
      541 ggatgcagtt tttgatcacc tgcgccagcc cctggagcac ctggttgccc ttttcgtggc
      601 cgtaggtgtc gttggttttc ttgaaccagt cgatgtcgat catcacgagc gagagcggct
      661 ggctgtagcg gttggcgcgg cggagttcgt cggtcaactt catggtgaag tagcggaagt
      721 tgaaggtgcc ggtcagttcg tcgacaatgg tcagttcttc cattttattg tgcatgacgg
      781 cgttctccag ggccagggcc gcggaggaag ccagaatcga aaagattttc tggtcgcgct
      841 cgaagaaggc gctcatctgc tccgactcgg cgttgaggac gccgatgaca ttcccgcgcg
      901 agatcatcgg caccaccatc tcggaacggg cccccggcag gccggggatg tagcgcgggt
      961 cgatgcgcac gtccgacacc cgctcggcct ttcccgaccg ggccaccgaa ccggcaatgc
     1021 cgtccaggga aacgacgatc ggctccgggt agatctgctt ttcgcccgat tccagtttgg
     1081 ccatcaaaag caggcggtct tcccgggcgt tgagcaggag caccgagcag agggggtagc
     1141 ccaggatgcg gcgggcgatg ttcaaaatct cgtccaacac ttcgtgcata ttgtgcattt
     1201 cccccaggga gcgggaggtt tcatagatcg tttcgatctg cacctgggag tgttccagtt
     1261 cggtcgtccg ttcgttcaag gtatccaggg cgcgcaggag gcggcgctcc gacccgcgca
     1321 ggtacttggc gataagcccg actgccgtcg cgaagaacca gaagaggaga atccggagca
     1381 ggaggtcgcc caggaaaaga tcggagaagg tgacggcgtt ggtcccgagg taggcgaccg
     1441 tggagagtcc ggcggccaag agcccgaagg ccatgccgaa gtagaacgaa gcaaaagaaa
     1501 tcgccagaag gtagaagagg tagaagttgc tcgtcaggcc gcccgtggcc caggttaaaa
     1561 gggtgatgaa ggcgaggtcg aagaaagttg taacccggta gagcgccggc agggaaacga
     1621 agttgcgcgc cgccagccag tagaagatca acaggtgcag gccgaaggcg atcaaaagtt
     1681 gctgcaccag gccggcattc acctcccggt ggggggaata gaccagccag gcgatgacgc
     1741 caaagatcgt caccacgcgg ctggagacga agactaactg caggcgcgag agtacggagc
     1801 gcggaaacgg ttcacgcatt gccatcgatc aacatcatcc tgaaaaagac acacccatcc
     1861 atccgctgcg agccttcttc ctaaatacaa agccagacgc ggcgggtgtc aatccctacg
     1921 ggtcgtaccg accgctgttc agtatatcgg cagtttccca taaattctac ggaagcggcg
     1981 ggcataccga cacatcccaa taacccgttt tccatcaacg ggttggagcc ttacgatttt
     2041 ctctacttat taggatgagg gaacttccga agccgcgtgg tgctaaattg aacagaacta
     2101 cagtggatat tttgcgttgc acaattcatc ggcaaggtga tgaattactc cgggcacttg
     2161 ccttttagca agagaatcta cattaataag gggtcagagg agtacaaagt aaatcgcatt
     2221 tgcgcgtcaa tgcaagattg aagagggcga gcatataacc ggaggctac
//
LOCUS       DBNGZD010000065         1811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1328862, whole genome shotgun sequence.
ACCESSION   DBNGZD010000065 DBNGZD010000000
VERSION     DBNGZD010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1328862"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /gene="nusG"
                     /locus_tag="AC16GM_01765"
     CDS             <1..524
                     /gene="nusG"
                     /locus_tag="AC16GM_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR2922432.1"
                     /translation="HTYAGHEQKAKKYLEHAATAAGLADRIGQILVPTESVNEMKQGK
                     RATSLRKFLPSYILIEMDLDRETQALVVNTPAITNFVGGGGKPVPLKPDEVERILEQI
                     ETGSTREPKEIPYKIGDPIKVIDGPFSDFSGTVSEVNEEKKRVKVMVSIFGRPTPVEL
                     DFLQIQKQEKKRR"
     gene            529..954
                     /gene="rplK"
                     /locus_tag="AC16GM_01770"
     CDS             529..954
                     /gene="rplK"
                     /locus_tag="AC16GM_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR2922433.1"
                     /translation="MAKKIVATVKLQISAGQANPAPPVGPALGQHGVNIMEFCKAFNA
                     RTQSQTGLIIPVIITIFADKSFTFITKTPPASVLLLKAAKVEKGSGEPHRVKVGSVTR
                     AQVREIATVKMPDLNAASLEAAERLVAGTARSMGIEIVG"
     gene            958..1671
                     /gene="rplA"
                     /locus_tag="AC16GM_01775"
     CDS             958..1671
                     /gene="rplA"
                     /locus_tag="AC16GM_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR2922434.1"
                     /translation="MPLKSGKKYRKATEGLEPARRYPLAAAVKEVKERRFAKFDEAVE
                     VSVRLGVDPKQADQIVRGTVVMPHGTGKKVRILVLAKGEKEKEAQAAGADYVGAEEYV
                     AKIQQGWTDVDAIIATPDLMGQVGKLGKILGPRGLMPNPKSGTVTFDVAKAVKDLKAG
                     KIEYRVDKQGNIGTAIGKTSFSEQQLTENAQAFLAAVVRARPASVKGQYLRNVTLSST
                     MGPGVKVDPQEVLNLAKGL"
BASE COUNT          423 a          512 c          582 g          294 t
ORIGIN      
        1 cccacaccta cgccggccac gagcagaagg caaagaagta cttagagcat gcggcgacgg
       61 cggccgggct agccgaccgc atcggtcaaa tcctggtgcc gaccgagtcg gtgaatgaga
      121 tgaaacaggg caagcgggcc acgagcctgc ggaagttctt gccctcctac atcctgatcg
      181 agatggatct cgaccgcgag acccaggccc tggtggtcaa cactccggcc atcaccaact
      241 tcgtgggcgg cgggggaaaa ccggtgccgc tcaagccgga cgaggtggag cgcatcttag
      301 agcagatcga aaccggcagc acccgggagc cgaaggagat cccgtacaag atcggcgacc
      361 cgatcaaggt catcgacggc ccgttctccg atttctccgg cacggtgagc gaagtgaacg
      421 aggagaagaa gcgcgtcaaa gtgatggtct ccatcttcgg ccggccgacg ccggtggaac
      481 tggatttttt gcaaattcag aagcaggaaa agaaaaggcg gtaaggcagt ggcgaaaaag
      541 atcgtcgcaa cggtgaaact ccagatctcc gccgggcagg ccaatccggc ccccccggtg
      601 ggcccggctt taggccagca cggggtgaac atcatggagt tctgcaaagc cttcaatgcc
      661 cgcacgcaga gccagacggg gctcatcatt ccggtgatca tcaccatttt cgccgacaag
      721 tcgttcacct ttatcaccaa aacgccgccg gcctcggtcc tcttgctcaa agcggccaaa
      781 gtcgaaaaag ggtccgggga gccgcaccgg gtcaaagtcg gctcggtcac ccgggcgcag
      841 gtacgggaga tcgccaccgt gaaaatgccg gatctgaatg cggccagtct ggaagcggcc
      901 gagcggctcg tggccggcac ggcccgcagc atgggcatcg aaatcgtcgg gtaaggcatg
      961 cctttgaaat ccggtaaaaa ataccgcaaa gcgactgagg gactggaacc agcccggcgc
     1021 tatccattgg ccgccgccgt gaaagaggtc aaagagcgca ggttcgccaa gttcgatgaa
     1081 gcggtggaag tttcggtgcg gctgggggtc gatcccaagc aggccgatca gattgtccgg
     1141 gggaccgtgg tgatgcccca cggtaccggc aagaaggtgc gcattctggt tttggccaag
     1201 ggcgagaagg agaaggaagc gcaggcggcc ggcgcggact acgtcggcgc cgaggagtat
     1261 gtcgccaaga tccagcaggg ctggaccgac gttgatgcca ttatcgccac ccccgatctg
     1321 atgggccagg tggggaaact cgggaaaatt ttaggcccgc gcgggctcat gcccaatccc
     1381 aagtcgggca cggtcacctt cgacgtggcc aaggcggtga aagatttgaa agccggaaaa
     1441 atcgagtacc gcgtggacaa gcaggggaac atcggcacgg ccatcgggaa aacgtcgttt
     1501 tccgaacagc agttgactga gaacgcccag gccttcctgg cagctgtggt gcgggcccgg
     1561 ccggcgtcgg tcaaaggcca gtacttaaga aacgtcacgc tgtcttccac tatgggcccg
     1621 ggggtgaaag tagaccctca ggaggtcctg aacttagcca agggattgtg agactatgaa
     1681 gcgagaagca ccggcgactt ttcccgttga tgttcacgaa ccccgaccgg aaaaaaaggc
     1741 ggcggtggaa caattggcgc agaaattccg ctccagcggt tcgttctttt tgaccgactt
     1801 caccggtttg a
//
LOCUS       DBNGZD010000066         3148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1676295, whole genome shotgun sequence.
ACCESSION   DBNGZD010000066 DBNGZD010000000
VERSION     DBNGZD010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1676295"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..777
                     /locus_tag="AC16GM_01780"
     CDS             133..777
                     /locus_tag="AC16GM_01780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR2922435.1"
                     /translation="MKIGILGTGDVGQTLGRGFAQLGHDVKMGSRNPNQEKVKSWLAK
                     TGARVSAGTFAETAAFGDLAVLATAWSGTESAIRLANPKNLARKVVIDATNPLDFSSG
                     APPKLGVGHTDSGGEQVQRWLPGASVVKAFNTVGSPHMVNPEFPGGPPEMFICGDDTG
                     AKQKVTELLHAFGWSVVDIGGIQGARYLEPLAMVWILHYFNTNSGNHAFKLLRK"
     gene            1146..1218
                     /locus_tag="AC16GM_01785"
     tRNA            1146..1218
                     /locus_tag="AC16GM_01785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1179..1181,aa:Thr,seq:ggt)
     gene            1288..2493
                     /gene="tuf"
                     /locus_tag="AC16GM_01790"
     CDS             1288..2493
                     /gene="tuf"
                     /locus_tag="AC16GM_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR2922436.1"
                     /translation="MAKPKYERTKPHVNVGTIGHVDHGKTTLTAAITMVLNRKGLAQV
                     RTFDSIDNAPEERERGITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDAVDDPELLDLVELEVRDLLKK
                     YEFPGDKLPVIRGSALQAMTQAIKPEVKDDPAFKCVYELMAAVDSYIPTPKREIDKPF
                     LMPVEDVFSITGRGTVGTGRVERGKVKVSDELERVGFRETKKTVVTGVEMFRKTMDYA
                     EAGDNVGLLLRGIEKEDLERGMVLAKPGSITPHTKFKGEVYVLTKEEGGRHTPFFTGY
                     RPQFYFRTTDVTGVIKLPEGVEMVMPGDNITMEVELITPIALEKELRFAVREGGHTVG
                     AGVVGTILE"
     gene            2548..2700
                     /gene="rpmG"
                     /locus_tag="AC16GM_01795"
     CDS             2548..2700
                     /gene="rpmG"
                     /locus_tag="AC16GM_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR2922437.1"
                     /translation="MAREIITLACGECKRRNYSGTKNKKKHPDRVEFKKFCPFCSRHT
                     PHKEVR"
     gene            2788..2861
                     /locus_tag="AC16GM_01800"
     tRNA            2788..2861
                     /locus_tag="AC16GM_01800"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2822..2824,aa:Trp,seq:cca)
     gene            2913..3107
                     /gene="secE"
                     /locus_tag="AC16GM_01805"
     CDS             2913..3107
                     /gene="secE"
                     /locus_tag="AC16GM_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR2922438.1"
                     /translation="MGGWIEKITRYLKEVRAELQKVSWPSRKELNASTVIVIFVSLVL
                     ALFIFGVDNVLSMVLSLVIK"
BASE COUNT          702 a          856 c          977 g          613 t
ORIGIN      
        1 agttggctag agtcacagcc ttccaagctg ttggtcgcgg gttcgagtcc cgtctcccgc
       61 tttgagtctt tcgagcgcaa cagcgacttt tttccgttgc attattcatt gatcaccaga
      121 gggagaaatg gtatgaagat cggtattctg ggaaccggcg acgtcggaca gacactggga
      181 agaggattcg ctcaactcgg gcacgacgtc aaaatgggtt cgcgcaatcc caaccaggaa
      241 aaggtcaaat cttggctggc gaaaacgggt gcgcgcgttt cggcggggac ttttgcggaa
      301 accgccgctt tcggagacct ggccgtgctg gccacagcct ggtcaggcac tgagagcgcc
      361 atccgcttgg ctaaccccaa aaacctggcc agaaaggtgg tcattgacgc caccaaccct
      421 ctggattttt cttccggggc tccgcctaag ttaggcgtcg gccatactga ttccggcggg
      481 gagcaggtgc agcgctggtt gccgggtgct tccgtggtca aagcgttcaa taccgtcggc
      541 tccccccata tggtcaaccc ggagtttccg ggcggaccgc cggagatgtt catctgcggc
      601 gacgacaccg gggccaagca gaaagtaact gaactcctgc acgcgttcgg gtggtcggtc
      661 gtcgacatcg gcgggattca gggcgcccgg tatctcgaac ctttggccat ggtctggatc
      721 ctccactact tcaatacgaa cagcggcaac catgccttca agctgctgcg taagtaacgg
      781 actggctctc tgggccggat tgatcacgtg ttcgcctgtc cgatgtatgc tttaaggtta
      841 cggccaggga gtcccgtctc ccggtttgaa gtctttggat aagtaaagaa gatggagata
      901 gtcctgcgcc aagagggtgg ttctggaggc gcggccgcga aaatttaccg gggcccaaaa
      961 aggacttgac aaaaaaatga aggggtgtat aataataact tacgtgctct gctaacttgc
     1021 agaatgccgg ttggtggggt atacttgttt gttcggctca ggggaaagag catattcaca
     1081 tccagcatca aatgctcccc aataagccgg cacaacggga agcatttgaa acggtgacaa
     1141 ggactgccca gatagctcag ttggtagagc acgtccttgg taaggacgag gtcaccggtt
     1201 caatcccggt tctgggctga attacggaaa cgcgccgaag acggaaaacg aaagtaaagg
     1261 gacgaagcag aggctgaggg agacggtatg gccaaaccaa agtacgaacg aacgaagccg
     1321 cacgtaaacg tcggcaccat cgggcacgtg gaccacggca agaccacgct caccgccgcc
     1381 atcaccatgg tgctgaaccg caaagggctg gcacaggtcc ggacgtttga ctccatcgac
     1441 aacgcgccgg aggagcggga gcggggcatc accattgcca cggcgcacgt ggagtacgaa
     1501 accgacaaaa ggcattacgc ccacgtcgac tgccccggac acgccgacta cgtcaagaac
     1561 atgatcaccg gcgcagccca gatggacggc gcgatcttag tcgtttccgc cgccgacggg
     1621 ccgatgcccc agacgcgcga acacatcctt ttggcccggc aggtgggcgt gccgtacatc
     1681 gtggtgttcc tcaataaagt ggatgcggtg gatgatccgg aacttttgga tctggttgag
     1741 ctggaagttc gggacctcct gaagaagtac gagttccccg gcgacaagct tccggtcatc
     1801 cgggggtcgg ccctgcaggc gatgacccag gccatcaagc cggaggtgaa agacgatccc
     1861 gcgttcaagt gcgtctacga actgatggcg gcggtcgatt cctacatccc cacccccaag
     1921 cgcgagatcg ataagccgtt tttgatgccg gtggaggacg tcttctccat caccgggcgc
     1981 ggcacggtgg gcaccggccg ggtggagcgg ggcaaggtga aagtgagcga cgaactggaa
     2041 cgggtcggtt tccgcgaaac gaagaaaacc gtggtcaccg gggtcgagat gttccgcaag
     2101 accatggact acgccgaagc gggcgacaac gtcggtttgc tcttgcgcgg cattgagaag
     2161 gaagacttag agcgcggcat ggttttggcc aagcccggtt ccatcacccc gcataccaag
     2221 ttcaaagggg aagtctacgt cctgaccaaa gaagaaggcg gccggcacac gccctttttc
     2281 accggctacc ggccccagtt ctacttccgc accaccgacg tgaccggcgt catcaaactg
     2341 cccgaagggg tggagatggt catgcccggg gacaacatca cgatggaagt ggagctgatc
     2401 accccgattg cgctggagaa ggaactgcgc tttgccgttc gcgagggcgg ccacacggtg
     2461 ggcgccgggg tcgtgggaac gatcctggag taagggctct gcggggcgca ggcccttgaa
     2521 tttttgtctc tgaatgcgtg gtaaagtatg gcgcgagaaa tcattacttt ggcgtgcggg
     2581 gaatgcaagc ggcgcaacta ttcgggcacc aagaataaaa agaagcatcc tgaccgcgtg
     2641 gagtttaaaa agttttgccc attctgcagc cggcacacgc cgcacaaaga ggtgcggtaa
     2701 tcccgcctgt cggagactcg tcgtagactc gactcttcga gactcttcga gaggcggccc
     2761 ccggacgcaa gcgagtgaca gattcttagg actgtagctc aactggccag agcaccggtc
     2821 tccaaaaccg ggggttgggg gttcgagtcc ctccagtcct gttgggtgtc ccgcccctgg
     2881 cgggattcga aagatcggtc gagaggcaac atttgggcgg atggattgaa aagataaccc
     2941 ggtacctgaa ggaggtccgg gccgaactgc agaaggtgag ctggccgtca cgcaaagagc
     3001 tgaacgcctc cacggtgatc gtcattttcg tttcgctcgt tctggcgctc ttcatcttcg
     3061 gcgtggataa cgttctttct atggttttga gtctggtgat taagtgatgg cgatgcggtg
     3121 gtacgtggcc cacacctacg ccggccac
//
LOCUS       DBNGZD010000067         3265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1606551, whole genome shotgun sequence.
ACCESSION   DBNGZD010000067 DBNGZD010000000
VERSION     DBNGZD010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1606551"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="AC16GM_01810"
     CDS             complement(<1..1646)
                     /locus_tag="AC16GM_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2922439.1"
                     /translation="MRIRHTLFLGAVGFFVLFAFVSSYLWAGNTGKIVGRVVDKETKE
                     GVVGATVRVDGTSLGAASDAEGKFFILSVPPGKYRVTITGVGYISLGVTNVTVVTDQT
                     TDLGTLKVEPSAIEMPAQKVVGAPPLINPNVTANVRNLSAEDIKNLPVKTVEDLLRLQ
                     VGVTKQGDVIHVRGGRGNEILYVVDGVEVRDPLGGRGPVGQALNVASTSIEELSIIKG
                     GFDAEYGNAQSAVVNISTKEGEVKTTRGHIEYFTDDFGSSSLDKYSYNFDRLEASLSG
                     PDPFLTTRFLPALGISGLEDKLTYFLSADADKTDTYLSFAKLASPTTQPTFRNYRFLG
                     LNIPDRQQNSYTTNMKITYKATPNLRFALNYKGSWDRYHNYQPGAGAGGTGGWYYRYS
                     PFTQALNREWGSVYSLNATHNVSKNTFYQVVLSRFFRRYFEAPSDPNRPGEVLLPNQF
                     RLADTQYEVYDDLNHNGHWDDPEPFTDLNHDGRYTFGDVFDDQNGNGKYDPGIDILLW
                     DWNRNGQYDQTEGEPFVDLNGNGVRDAGDNLRVDLNGNGR"
     gene            complement(1815..2582)
                     /locus_tag="AC16GM_01815"
     CDS             complement(1815..2582)
                     /locus_tag="AC16GM_01815"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR2922440.1"
                     /translation="MALDQVLLQSESNSGSVFTLRFYGWRPPALSLGYFQRIEPAVAE
                     RCRARGIDVVRRLTGGRAVLHDRELTYSVTGPVNGVLGRSVREIYLKIGEALVLGLQK
                     LGAAAELVDPHGNKPESRRGYVMKAACFASQTQGEIAVGKMKVVGSAQRQVGGRFLQH
                     GSVPLAPPRVAIEDLFGVELPEKNRSSMTLTEASGRPVGYREVADALKASFAERFAVT
                     WQEAGLTPAERQTAGRLAEERYRRMDWSGQNQKLAVV"
     gene            3005..>3265
                     /locus_tag="AC16GM_01820"
     CDS             3005..>3265
                     /locus_tag="AC16GM_01820"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR2922441.1"
                     /translation="MDNKKKIIEMLNEDLGDELASIIQYMWHHVMVAGPTAPGAKEEF
                     MKAARDEMKHAEMLAEKVNYLGGVPTTRHGEIRVGGDLKKMLN"
BASE COUNT          686 a          980 c          891 g          708 t
ORIGIN      
        1 tagcggccgt tgccgttcag gtcaaccctg aggttgtcgc ccgcgtcccg cacaccgttg
       61 ccgttcagat ccacgaacgg ctccccctcg gtctgatcgt actgcccgtt gcggttccag
      121 tcccatagga gaatatcgat gccggggtca tacttcccgt tgccgttctg atcgtcgaaa
      181 acatcgccaa aggtatatct cccgtcgtgg tttaagtcgg tgaacggttc ggggtcgtcc
      241 cagtggccgt tgtggttcaa gtcgtcgtac acttcgtact gggtgtcggc caggcggaac
      301 tggttcggca aaagcacttc gcccggccgg ttggggtccg acggggcttc gaagtaccgc
      361 cggaagaagc gcgacaagac cacctggtaa aaagtattct tgctcacgtt gtgggtggcg
      421 ttcaagctat agacactccc ccactcccgg ttgagcgcct gcgtgaaagg cgaatagcgg
      481 tagtaccacc ctccggtgcc gcccgcgccg gcgccgggct ggtagttgtg gtagcggtcc
      541 caggagccct tgtagttcaa ggcaaaacgc aggttcggcg tggccttgta ggtgatcttc
      601 atattggtcg tgtaggaatt ctgctgccga tcggggatgt tcaagcccag gaacctgtag
      661 ttccggaagg tcggctgcgt ggtgggtgaa gcgagcttgg caaaggagag gtaggtgtcg
      721 gtcttgtcgg catcggcgga gaggaagtag gtcaatttgt cctctaagcc ggaaatgccc
      781 aaagccggca aaaagcgcgt ggtcaggaac ggatcgggac cggagaggct ggcctccaac
      841 cggtcgaagt tgtaggagta cttgtccagg ctgctcgagc cgaaatcgtc ggtgaagtac
      901 tctatgtgcc cccgggtggt tttgacttct ccttctttgg tgctgatgtt caccacggcc
      961 gactgcgcgt tgccgtactc ggcgtcgaaa ccgcctttga tgatcgagag ctcttcaatc
     1021 gaggtgctgg cgacgttcaa ggcctgcccc accggcccgc ggccgcccaa ggggtcgcgg
     1081 acttccacgc cgtccaccac gtagaggatt tcgttgcccc ggccgccccg gacgtggatc
     1141 acgtcgccct gcttggtcac ccccacctgg agccggagca aatcctccac cgtcttcacc
     1201 ggcaggtttt tgatgtcctc ggccgaaagg ttccgcacgt tggcggtcac attggggtta
     1261 atgagcggcg gcgcccccac taccttctgc gccggcattt cgatcgccga gggttcgact
     1321 ttcaaagtcc ccagatcggt cgtctggtcg gttaccacgg tgacattggt aacccccagg
     1381 gagatgtaac cgacgccggt aatagtgaca cgatattttc cgggcgggac gctcaggatg
     1441 aagaattttc cctccgcgtc ggaggccgcg cctaagctgg tgccatccac gcggacggtg
     1501 gcgcccacca ctccttcttt ggtctctttg tctaccaccc ggccgacgat cttcccggtg
     1561 ttcccggccc agaggtatga ggaaacgaac gcaaacagga caaaaaatcc caccgccccc
     1621 agaaacaacg tgtgacgtat ccgcattgct ctcatcctcc ttcaaacgag gcgtttattt
     1681 tgcaactttc tttgggccaa tcccgtaaaa gaaaagtttg acgaccatcg gctaatctgc
     1741 tgaccttccc gtattttgtc aagtcctttt ttggccacag aacgcgcgga ggcttgttgg
     1801 acggcccttc tttctcagac aaccgccagc ttttggtttt gcccggacca atccatacgg
     1861 cgatagcgtt cctcggccag ccgaccggcc gtctgccgct ccgccggagt caatccggcc
     1921 tcctgccacg tcacagcaaa tcgttccgca aaacttgcct tcagcgcgtc agccacttcg
     1981 cggtagccca ccggacgtcc ggacgcttcg gtcagggtca tgctcgaccg atttttctcc
     2041 ggcaactcga ccccgaacaa gtcttcaatc gcgaccctcg gcggtgccaa aggtaccgac
     2101 ccgtgctgta aaaacctgcc gcctacctgc cgttgcgcgc tgcccacaac cttcattttc
     2161 ccgacggcga tttctccctg cgtttgggac gcaaagcagg ccgccttcat cacataaccg
     2221 cgccgggact ctggtttgtt cccgtgggga tcaaccagtt ccgcggccgc acccagcttt
     2281 tgcagtccca gcactaaggc ctcaccgatc ttcaggtaaa tctcccggac tgaccggcct
     2341 aaaacgccgt tcaccggacc ggtgaccgag taggtcagtt cccggtcgtg gagcaccgcc
     2401 cggccgccgg tcaagcgccg caccacgtct attcctctgg cccggcaccg ttccgccact
     2461 gccggctcta ttctctggaa gtaccccagt gacagagcgg ggggtctcca tccatagaac
     2521 cgcagagtga atacggagcc ggagttgctc tcggattgta aaagaacctg gtcgagtgcc
     2581 atattcaatg ggccggaatt aaacccgcta tccagaaaac gccaatgctt ttccgcaccc
     2641 ggagacactc cgacacctcc aaatcagtca aaccctatgc cgagattatc tctacccaaa
     2701 aaaattatca cgttggaatt taacgactta caactcgaaa aaatttgttt catactgatc
     2761 accgacgaat tttggccgat attccttttc gattctttaa tctcaagttt aactgtatga
     2821 ctttcaggcg gttactggaa attaaaccta tcaattcgaa aattgccgaa atacgaaatt
     2881 ccgccgaggg cttcggcatt ccatcaggtc tatgtccccg ccaaaaagcc cataaagtgg
     2941 ttgaccgtcg ctaaggaaca aattatgtta gcggcggaaa gtcaaccgat ttcctggagg
     3001 aaaaatggac aacaaaaaga aaataatcga gatgctgaac gaagacctgg gcgacgaatt
     3061 ggcctcgatt atccagtaca tgtggcacca cgtgatggtc gccggcccca cggctccggg
     3121 cgccaaagag gagtttatga aagccgcgcg ggacgaaatg aagcacgccg aaatgttggc
     3181 cgagaaggtc aactacctgg gcggcgtccc tacgaccagg cacggggaaa tccgcgtcgg
     3241 cggtgatttg aaaaagatgc tcaac
//
LOCUS       DBNGZD010000068         2288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_148168, whole genome shotgun sequence.
ACCESSION   DBNGZD010000068 DBNGZD010000000
VERSION     DBNGZD010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_148168"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="holA"
                     /locus_tag="AC16GM_01825"
     CDS             <1..833
                     /gene="holA"
                     /locus_tag="AC16GM_01825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR2922442.1"
                     /translation="TNAEEVINAVSTAPMFGTHRLVEVHDAQRLSPPAKERLIALLPK
                     IPETGVTIFLADKSDFRQVFWKFFLDQGAAYEFASLLEKQALEWLFQRAKRSGIEVEE
                     EAAWALVEKLGTDAGQIASELEKLATLAQKSQKITKSLVEQAVGFSQRFSVYQLLDAV
                     GERNLPQALVIYNDLVSYRESLTQIVYWLGEHFYRLYVVTQSDNGKLADPRVAALHPF
                     VLNKCRRQAKNFSVGQLSSGLSQIAQADADIRLSKVKSELVVETLLVRLCRLSGDKRE
                     "
     gene            858..1457
                     /locus_tag="AC16GM_01830"
     CDS             858..1457
                     /locus_tag="AC16GM_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2922443.1"
                     /translation="MKSEGEAVRDVATPALPEEAALVRRARGQDKKAFAELVRIYQKR
                     IWALVYRLTKERDAAWDLSQDTFVKAYFAIGSFREGMPFYPWLATIAVHLALNYLKRE
                     KRQVKFTDEEMQEHVLESQPATSNPIEEMISAEMQAKLEGEIDRLPPEYRAVFVLRVY
                     EEMSYDQIAKTLEISQGTVMSRLFRARQRLLAALKEHLA"
     gene            1474..>2288
                     /locus_tag="AC16GM_01835"
     CDS             1474..>2288
                     /locus_tag="AC16GM_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR2922444.1"
                     /translation="MTTNRCGFDLAKLSAYVDGALSPKDHLTVEAHLQTCPDCREFIA
                     GLQKAEASLKGAPAPELTPEEEARFAPAVMRQIERLEQKPAPVSAGAKKFFLFRSVGL
                     RWAAAVAAVVLVAITSTLLLKEKSELLQPQRRLGQRTPEGRLAPPLTTPETTQTGLIG
                     NESLAKNEETIPAQEAAKKPVAESSRVAESLEATPQAELAVPAPAPEESLGEGARVEE
                     PAKVEDKDLAAQKKVPEKGEPLFVNPQIAPRQLAAETPETTTTRARDVLHMRGG"
BASE COUNT          524 a          656 c          663 g          445 t
ORIGIN      
        1 agaccaacgc cgaagaggtc atcaacgccg tctccaccgc tcccatgttc ggcacgcacc
       61 gcctggtcga agttcacgac gcccaaagac tgtcgcctcc ggcgaaggag cgtctgatcg
      121 ccttgctccc gaagattccg gaaaccggtg tcaccatatt tctggcggac aaaagcgatt
      181 tccggcaggt tttctggaaa tttttcctgg accagggtgc ggcctacgag tttgcatccc
      241 tgctggaaaa gcaggcgctc gagtggcttt tccagcgcgc caagaggtcg ggcattgaag
      301 tggaagagga ggccgcctgg gcgttggtcg aaaaattggg caccgatgcc gggcagatcg
      361 cttccgaact ggaaaagttg gccacgttag cacaaaaatc ccaaaaaatt acaaaatctc
      421 tggtggaaca ggcggtggga ttttcacagc gattctcggt ctaccagctc ttagatgccg
      481 tgggcgagcg caacctgccc caggcgctcg tcatctacaa cgatttggtc tcctaccgtg
      541 aatcattaac ccaaatcgtc tactggctgg gggagcattt ctaccggctc tacgtggtca
      601 cccaaagcga caacggtaaa ctggccgatc cccgggtggc ggccctccat cccttcgttt
      661 tgaacaaatg ccgccggcag gccaaaaact tttccgtcgg tcaactctct tccggcctca
      721 gccagattgc ccaggccgat gctgatattc gactctccaa ggtcaaatcg gaactggtgg
      781 tggagaccct tttggtccgg ctctgccgtc tttcgggaga caaacgggaa taaaacgccg
      841 ccccggctgt atttcctatg aaaagcgaag gtgaagcggt tcgagatgtc gccaccccgg
      901 cactgcccga agaagccgcg ctggtgcggc gcgctcgcgg acaagataaa aaagcgtttg
      961 cggagctggt ccgaatctat caaaaacgca tctgggcttt ggtctatcgc ctgaccaaag
     1021 aacgcgatgc cgcctgggat ctgtcgcagg atacttttgt gaaggcctac ttcgccatcg
     1081 gctcgttccg ggaagggatg cctttctatc cctggctggc caccattgcg gtgcatctgg
     1141 ccttgaatta tctcaaacgg gagaagcgcc aagtcaagtt taccgatgag gaaatgcagg
     1201 agcatgtttt ggaaagtcaa ccggcgactt caaatccgat agaggaaatg atcagcgcgg
     1261 aaatgcaggc caaactggag ggtgaaattg accgcctgcc gccggagtat cgcgccgtat
     1321 tcgtgttgcg ggtttacgag gagatgagtt acgatcagat cgccaagacg ctggagattt
     1381 cccagggtac ggtgatgtcg cgcttgttcc gcgcccggca gcgccttttg gccgcgctca
     1441 aagaacattt agcttaaggc aaaacaggag accatgacca ctaatcgctg tggatttgat
     1501 ttggcaaaac tttccgccta cgtggacggt gctctgtctc ccaaggacca tttgacagtc
     1561 gaagcgcacc tgcagacctg cccggactgc cgggagttta tcgccggtct ccaaaaggcg
     1621 gaagcatcac tcaagggggc acccgctccg gaactgaccc cggaggagga agcccgcttc
     1681 gctccggcgg taatgcgcca aattgagcgg ctggaacaaa agccggcccc ggtctcggcc
     1741 ggggcaaaaa agtttttcct cttccggtcg gtgggtttgc gctgggccgc cgcagtcgcg
     1801 gccgtcgttt tggtggcgat caccagcacc ctattactca aggagaaatc tgaactcctg
     1861 caaccgcagc ggcggttggg ccagaggaca ccggaagggc gcctggctcc gcctctgacc
     1921 acgccggaga cgacccaaac gggtttgatc gggaatgaat cgctcgccaa gaacgaggaa
     1981 acaatccctg ctcaagaagc ggcgaagaaa ccggttgcag aatcatctcg agttgcagaa
     2041 tcactggagg caactcccca agccgagctc gcagttcccg cacccgcccc ggaggagtcg
     2101 ttaggggaag gtgcgcgggt ggaggaaccg gcaaaggtcg aagacaaaga cctcgcggct
     2161 caaaagaagg tcccagagaa aggagaaccg cttttcgtca atccccagat tgccccccgg
     2221 caactcgcgg ctgaaacacc ggaaactact accacccggg ctcgcgatgt cttgcacatg
     2281 cggggtgg
//
LOCUS       DBNGZD010000069        12998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1259452, whole genome shotgun sequence.
ACCESSION   DBNGZD010000069 DBNGZD010000000
VERSION     DBNGZD010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1259452"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /gene="bamA"
                     /locus_tag="AC16GM_01840"
     CDS             complement(<1..1352)
                     /gene="bamA"
                     /locus_tag="AC16GM_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR2922445.1"
                     /translation="MCLWALPAFSQTQEYQIVSVEVQGNRVAERSLVQNVAGLAVGSR
                     LDAGGIADAIRRIYGLNLFSDVAIEGDLPGNNGIALVIQVQEYPKIRRIDIAGNKKVK
                     TEEIRTKLTLREGQTASPVAVQENVNRIRSLCEEKGYYLAAVESKSATEGSADEVVLS
                     FRINEGEKVKLEFIEFAGASAYPPDKLRGKIHGGGFKAERYEQDKEKLIEFYQKNGYT
                     DAAILGDTTWVDSTRRFMHALITLGEGKRFHFGQVAFSGDSLFGQQRLASQVKFKPGE
                     IFNQKKYDQTLGNLYALYQEEGYLYARIEDRKEPQDSLVNLTYEITQGIPAKINRVII
                     EGNQKTKDKVIRRELSVRPGQTFRRSLLLRSVRDVMQLNYFANVTPDYAVQPTGDIDL
                     VIKVEEKPTGQIQVGAGYSGQDKLVGTIGLGIPNLFGNGQRLDLNLDFGNRRNSVSLG
                     "
     gene            complement(1579..1902)
                     /gene="rplU"
                     /locus_tag="AC16GM_01845"
     CDS             complement(1579..1902)
                     /gene="rplU"
                     /locus_tag="AC16GM_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR2922446.1"
                     /translation="MYAIVEIGGMQFPVREGEVVEVPRLGAKAEDQVTFDKVLLVAGE
                     KGTAVGNPYLPKASVLGEVVREARGEKIIVFKFKRRTKYRRKRGHRQWYTAVKINKIQ
                     APQIA"
     gene            2050..3594
                     /locus_tag="AC16GM_01850"
     CDS             2050..3594
                     /locus_tag="AC16GM_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922447.1"
                     /translation="MVGIRFGEKNVLLVVGLAALAVAAWTAFASSGTWEDDAYIFFQY
                     AKNLAAGQGMAFNSGEVSFGVTSVLWTLILGFLSWVFHLGPVVMAKLASIVLFSASVT
                     ILTRLVIDCTQNSLLGFWTGLLVAVYPHLVLHAVSGMEIPLNLFLLTVLTVSFFRYGE
                     KHFWLYGILIGLAFLTRPENALWLPLFVWLAATSRGQFGLRIAALLGTCVGVVLPWEL
                     YLYSHTSLFLPPTRDGKLLLFLPVQYGVTLDQFRHLSLAARLDLAGRASVNVAKAIFL
                     NKGGVLFLPFLLVAGYFVARGKIALGRFGLALAGYGFGLVALFVLFFPLVKQRYFVHL
                     YPFLIFISVLTGCRLWQELRGRFKILIRPLWAKVGLVTVLLIAPLAGFFAARKFASMS
                     REQSIRAEVGRWLKTNTPPDARVALEPIGAVAYFADRYVVDLGGLINPDIWPFMSRGP
                     DSPPDALFDILVRKRVTHVVDYSQHPWAGKVVDLYPERFLLAAQISSPYPPAGYNQYD
                     IYKLNK"
     gene            3869..6322
                     /locus_tag="AC16GM_01855"
     CDS             3869..6322
                     /locus_tag="AC16GM_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR2922448.1"
                     /translation="MNEMFSERARRAIEYARDEAARLKHDYIGTEHLLLGLLKLGEGR
                     AIDVLTNIGLDLAELKQGIEDVVQPSGGTMVMGQLPLTARAKKTLEVSGQEARALKSK
                     DIDTEHILLALLKEEEGVAAQVLSMYDVDYKEVYEELKNIQSGKPSAYGRKRKKSKTP
                     ALDHFGRDLTELARRSKLDPIIGRDNEIERVSQILSRRKKNNPVLIGEPGVGKTAIVE
                     GMAQRIVEGRVPQTLENKRVVTLDMAALVAGTKYRGQFEERMKAVMNEIINSRDVIIF
                     IDELHTIVGAGGAEGSLDASNIFKPALSRGELQCIGATTLNEYRKYIEKDGALERRFQ
                     TVMVDPPSPGDTIKILEGLKSRYEDHHKVRITDEAIAASVKLSDRYISGKFQPDKAID
                     VLDEAGSRAHLASFTKPVTFTDIEQEIARIQRDKEHSVKNQEFERAAQLRDELKFKKD
                     ELEKARRAWEEERSKQVVELKAEEIAQVVSKMTGIPVFRLEEVESKKLLRMEEELTKR
                     VVGQSEAINVISKAIRRSRAGLSDPRRPIGSFIFLGPTGCGKTELARALAAFLFEDEE
                     ALVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEKVRRKPYSVVLLDEIEKAHPE
                     VFNLLLQVLDDGTLTDSFGRKVDFKNTVVIMTSNIGAREIHAGKHLGFVPEEEQFSYE
                     RMRQKITEEVKKIFNPEFLNRIDDMVVFHPLGRPEILKIVDIQLKEVAQRLAEKEVSF
                     TLTDAAKEFLADKGFDPVFGARPLKRAIQKYLEDPMAEEILKGQFGTECEVLVDKGEG
                     DGLRFTIAGGRPTLVPVKK"
     gene            complement(7152..9512)
                     /locus_tag="AC16GM_01860"
     CDS             complement(7152..9512)
                     /locus_tag="AC16GM_01860"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HIR2922449.1"
                     /translation="MLKKFSGNLLYILITLLVLTLYLDHHDSLAKYEVKIQDLMLKFR
                     GKSDPGKDVVVVAVDDQAINQIGHWPWPKATMARFLDALSAQKPKVIAGNFPLPFTAP
                     DTGAVTDSLADVIKRAKNVILPVSFTLAETGVNTITTPAYITSSAYLIYGEEFDLQDN
                     PPRLAREIVYPEAPLDSAAFGLGHTAFAPDPDGMLRWAPLIVKYEGFYYPSLAVQAAR
                     AYYGLVRQQVKIETGKKIWLDNLSIPIDDHGQMLIAFRGPANTFPSVSAADVLSGRVD
                     PSALRDKVAVVGMTATGLTQQYKTAIARGIPRVEVTATVIENIIHRRFYSSLSLSTLL
                     DVGILILIGVFSALILPKVALLYRAVILAVFAFAILNFNFILFASFNLMPKVFYPMME
                     VFFFLAASFTIKPKKEGAQAEVETGEEEGYAPRQYQRFARQEEAEPPRPERARVEYGP
                     ASLELAEMHATQHMVSSDLATVELEMKTPEQMAAEETQMLVEAKAEAKKQAEAGQLGK
                     LGRYQVLQVLGKGAMGTVYKGLDPALDRLVALKTIRMDFASSPDEVTELRERLLREAK
                     AAGKLSHPNIVTVYDVGQDGDIQYIAMEYLQGYTLEEVIKKKVELNFRIVASIITQIC
                     NALEYAHDQGIVHRDIKPANIMVLNGFNIKVMDFGIARIATSNMTQSGVALGTPNYIS
                     PEQLTGQEIDRRADIFALGVVMYEFLTRKKPFTGESLSALIYNIINLTPPPPSQINDK
                     VPELFDRIILRALEKSVLYRYQRAGEIAEDLKTYLASFRSRPVRTY"
     gene            complement(9586..10314)
                     /locus_tag="AC16GM_01865"
     CDS             complement(9586..10314)
                     /locus_tag="AC16GM_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR2922450.1"
                     /translation="MKLGFFSDVHGNLEALEAVLADCAAEKLEKLFFLGDAVGYGPSP
                     NECVRLVAEAAEVSLMGNHDYAALGLLELDLFNHYAKQTLDWTRRVLDERAQEILSGF
                     QLDHRVADCYLVHASPHEPLNWDYVLTLEDAEHCFPFFREPVCLIGHTHRPALVKKTG
                     SQPCTLLADTGVKIEGGSRYLINIGSVGQPRDGNPKASYLVYDTERHQATLKRVAYDF
                     QKVQQKMAEFQVPTFLIERIATGR"
     gene            complement(10311..11006)
                     /locus_tag="AC16GM_01870"
     CDS             complement(10311..11006)
                     /locus_tag="AC16GM_01870"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR2922451.1"
                     /translation="MPEILVKYEDKIIERVVTEKKRISIGRTSDNDIVLDNRGVSRKH
                     AQIEFNGNTAVILDNESLNGTFVNNRKVAEEILKHNDVITIGKYQLFYHVAAGHDYKP
                     TDMDGTMVLKTKKQRELLEKDLRDRQIVTKYGGPVLQGVAHCEAEQFKLDRDVVTFGK
                     SSFVNIQVKGFWVSGIQAKLIREDSRFVLVNVGRRGRVKVNGEPVERVGLRNDDLLEI
                     GRSVFRFVEGSSR"
     gene            11236..12000
                     /gene="larB"
                     /locus_tag="AC16GM_01875"
     CDS             11236..12000
                     /gene="larB"
                     /locus_tag="AC16GM_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR2922452.1"
                     /translation="MHPETLARLLRQIRTRKISPPQALERLKLLPFEDLGFATIDHHR
                     HLRQGLPEVILGTGKTAPQVEKIAERLLAAGAPVLATRLDEPVRKILREHFPWARENA
                     LARAVFISSTKLAKKTRGGGRLLIISAGTADQPVAEEASFAGEVIGLRTKRAFDVGVA
                     GLHRFFAHTDSLRWADVIIVVAGMEGALPSVVGGVVGKPVIAVPTSVGYGTSFGGLTA
                     LFAMLNSCAAGVTVVNIDNGFGAAFAAWRILQVKPK"
     gene            11997..>12998
                     /gene="larC"
                     /locus_tag="AC16GM_01880"
     CDS             11997..>12998
                     /gene="larC"
                     /locus_tag="AC16GM_01880"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR2922453.1"
                     /translation="MKIAYCDCIGGAAGDMLLGALVASGLKLEALQKEIAKLSLPEVR
                     VTVHPVKRQALAAAQIEISAPEAQAYRHLKEIEQIISTSGLADAVKLKSLAIFHRLAE
                     AEARVHGSSVEEIHFHELGAVDTIVDVVGSVAGFETLGIEKIYSSPLPLGRGMIASEH
                     GPLPLPAPATAELVRNYPVRPVDIEGELVTPTGAAILTTLAEFTAPPPYRLLTVGYGA
                     GSRNLANIPNVLRLFIGETVPAPAVDTVTQIETNLDNTSPEVLGYLAERLFSAGALDV
                     YFVPVTMKKSRPGVLLGLLANPEDREKFAAILFEETGTIGLRYWECQRRKLPRSEEVV
                     "
BASE COUNT         2551 a         3841 c         3803 g         2803 t
ORIGIN      
        1 aaccctaaag agaccgagtt ccgccggttg ccgaaatcca gattcaaatc gagccgttgc
       61 ccgttgccga agaggttcgg aatccccaga ccgatggtgc cgactaattt gtcctgcccg
      121 ctgtagccgg cgcccacctg aatctggccg gtcggtttct cttcgacttt gatcaccaga
      181 tcgatgtcgc ctgtcggctg caccgcgtag tcgggggtga cgttggcgaa gtagttcaac
      241 tgcatcacgt cccgtaccga gcgcagcaaa agggagcggc ggaaggtctg ccccggccgg
      301 accgaaagct cgcggcgaat aaccttatcc ttggtctttt gattcccctc gatgatcacc
      361 cggttgattt tggccggaat cccctgggtg atttcgtagg tcaaattgac gagcgaatcc
      421 tgcggctctt ttctgtcctc gatccgggcg tagaggtacc cctcttcctg gtagagggcg
      481 tagaggttgc ccaaggtctg gtcgtacttt ttctggttga agatttcgcc gggcttaaac
      541 ttcacctggc tggccaaacg ctgctgaccg aacaaactgt ccccggagaa ggccacctgg
      601 ccgaagtgga agcgtttccc ttccccaagg gtgattaacg catgcatgaa gcggcgggtc
      661 gaatctaccc aggtcgtgtc ccccaggatc gccgcatcgg tgtagccgtt cttctggtag
      721 aattcgatta acttctcttt atcctgctcg taacgctccg ctttgaaacc gccgccgtgg
      781 attttgcccc gcagtttatc cgggggatag gcgctcgccc ccgcgaattc gatgaattcc
      841 agtttgacct tttccccctc gttgatgcgg aaggataaga ctacctcatc ggcgctccct
      901 tcggtcgccg atttgctctc gacggccgcc aggtagtacc ctttctcttc gcagagggag
      961 cggatgcggt tcacattttc ctggacggcg accgggctgg cggtctgacc ttcccgtagc
     1021 gtcaatttcg tccggatctc ttcggttttg actttcttgt tgccggcaat gtcgatgcgg
     1081 cggattttgg ggtactcctg aacctggatg accagagcga tcccattgtt tcccgggagg
     1141 tcaccctcga tggctacgtc ggagaataaa ttaagcccgt agatccgccg gatggcatcg
     1201 gcaatgcccc ccgcatccaa ccggctgccg acggccagtc cggccacgtt ctgaacgagc
     1261 gaccgctccg ccacccggtt gccctgcacc tcaaccgaga caatctgata ctcctgagtc
     1321 tgagagaaag ctgggagggc ccacaggcac acaaccacga gacgtggtag ccacgctctc
     1381 tttagcctga gtcccgccca gggcgggatc gaaggccgga gcgtggaatt tgcgcgatgc
     1441 cgatggggaa cgttttttct ccagttaata aaaaaccaaa ccggcccgcc ctccccctgc
     1501 gctgccggtt tatgcgtgcg ctccttgcgt ttcaaaggct tcatcccgtg tcctctggcc
     1561 tgctgataat acgcaggctc aggcaatctg gggagcctgt attttattga tttttacggc
     1621 ggtgtaccac tggcggtgcc cccgtttgcg gcggtacttg gtccggcgtt tgaacttaaa
     1681 gacgatgatt ttctccccgc gcgcctcccg caccacttcg cccaaaacgc tggccttggg
     1741 caggtaggga ttcccgaccg cagtgccttt ttcgccggcc accaaaagca ccttgtcaaa
     1801 agtcacttgg tcctcggcct tggcccccag ccggggaacc tcaaccactt cgccctcccg
     1861 caccgggaac tgcattcccc ctatctcgac gatcgcatac attcgcttcc actcctttgc
     1921 ttctcgtagg ttgtaagcat tggctaatat acaccttccc gatttcggac gtcaagggac
     1981 cgaccagcgt aaatttttga caagggtagg gctcttgcgt atatgaacgg cctcgagagg
     2041 actataccga tggttgggat acggttcggc gaaaaaaacg tgttgcttgt ggtggggctg
     2101 gctgctctgg ctgttgccgc ctggaccgct tttgcctctt cgggtacctg ggaagacgat
     2161 gcctacatct tctttcagta cgccaaaaat ctggctgccg gtcagggaat ggctttcaat
     2221 agcggcgaag taagcttcgg tgtcaccagc gtgctctgga cgctcatttt gggtttcctc
     2281 agctgggtct tccacctggg cccggtggtg atggccaaac tcgcatccat agtcctgttt
     2341 tctgcctcgg tgaccatcct cacccgattg gtgatcgact gcacacagaa ctctctgctc
     2401 gggttctgga cgggtctatt ggtcgccgtc tacccgcacc tcgtgctcca tgctgtcagt
     2461 ggcatggaaa tcccgctcaa cctttttctc ttgactgtcc tgactgtctc gttcttccgt
     2521 tatggggaga aacatttctg gctctacgga atcctcatcg gtcttgcctt cctcacccgc
     2581 ccggaaaatg ccctctggct gccgctgttt gtttggctgg cggcaacttc ccggggacag
     2641 ttcggcctga gaatcgcggc cctcctgggc acctgtgttg gagtcgtcct cccgtgggaa
     2701 ttgtacctct actcccacac ctctctcttt ctgccgccta cccgggatgg taagctcttg
     2761 ctcttcctgc ccgtccagta cggcgtgacc ctggaccagt tccgtcacct gagcctcgcg
     2821 gcccgacttg atctggctgg ccgggcatcg gtgaatgtcg ccaaggcgat ctttctcaac
     2881 aaaggtggtg tactgtttct cccctttctc ctagtggccg gttacttcgt ggctcgcggg
     2941 aaaattgctc tggggcgatt cgggctggca ctggcggggt acggctttgg tctggtggca
     3001 ctctttgtcc ttttcttccc tctggtgaaa caacgctact tcgttcacct ctatccgttc
     3061 ttgattttca tctcggtttt gaccggctgc cgcctgtggc aggaactccg tgggagattc
     3121 aaaatcctca tccgcccgct ctgggccaaa gtcgggcttg tcactgtcct gctcatcgcg
     3181 ccgttggccg gattcttcgc cgcgcgaaag tttgccagta tgtcccggga gcagagcatt
     3241 cgggcggaag tcgggcgctg gctcaaaacc aataccccgc ctgacgcccg cgttgcgctc
     3301 gaaccgatcg gcgcggttgc ctatttcgcc gatcggtacg tcgtcgatct cggaggactc
     3361 atcaaccctg acatctggcc tttcatgtcc cgcggaccgg attcgccgcc ggatgcactc
     3421 tttgacattc tcgtgaggaa aagggtgacc cacgttgtcg attacagcca gcacccctgg
     3481 gcagggaaag tcgtcgatct ctatccggaa agattcctac tggctgcgca gatctcctcg
     3541 ccttatcccc cggcgggcta caaccagtat gacatttata aactgaacaa ataaccccga
     3601 ctggttgccg atcacctccg caaccggcgt gcctgctggt gaacaaattc acagccgcct
     3661 gctctccctt tttcccccga tatgccgata tttcattgag gagcgtccgg cgcgaagccg
     3721 agagcgaaaa atcgctgtgg gcacaaaaaa atatcttgtc tccacgtgtg ccggacagta
     3781 tattcgccca gtacgcagcg tggctgcgta tatcatatag agccccgcct taggcggggc
     3841 ccaaaaacgt tcaaaggatt gaaaaacgat gaatgagatg ttttccgaac gcgcccggcg
     3901 ggccattgag tatgcccgcg acgaagcggc ccgattgaag cacgactaca tcggcaccga
     3961 gcacctcctt ttgggcttgt tgaagttagg cgagggtcgg gccatagatg ttttgaccaa
     4021 catcggcctc gatctggccg agttgaagca ggggattgag gacgtggttc agccctccgg
     4081 cgggaccatg gtgatggggc aactgccttt gaccgcccgg gccaagaaga ctctggaagt
     4141 ctcgggacag gaagcccgcg cgttgaagtc gaaagacatc gacaccgagc acattctgct
     4201 cgccctctta aaagaggaag aaggtgtggc cgcccaggtc ctctcgatgt acgacgtcga
     4261 ttacaaggaa gtctacgaag aactcaagaa catccagtcg ggcaaaccct cggcctacgg
     4321 ccgcaaacgc aagaagagca aaaccccggc tttggaccac tttggccgcg acttaaccga
     4381 actcgcccgc cgcagcaaat tggacccgat tatcggccgc gacaacgaga ttgagcgggt
     4441 ttcgcaaatc ctctcgcgcc gcaagaagaa taacccggtt ctgatcggcg agcccggcgt
     4501 cggcaaaacc gccattgtgg aagggatggc gcagcggatc gtcgaaggcc gggtgccgca
     4561 gaccctggag aacaagcggg tggtcacgct ggacatggcg gccctggtgg ccgggaccaa
     4621 gtaccgcggc cagttcgagg agcggatgaa agcggtgatg aacgaaatca tcaattcccg
     4681 ggacgtgatc atcttcatcg atgaactgca caccatcgtc ggcgccggcg gggccgaagg
     4741 gtccctggac gcctcgaaca tcttcaaacc ggctctttcc cgcggcgaac tccagtgcat
     4801 cggcgccacg actttgaacg agtaccgcaa gtacattgaa aaagacggcg ccctggagcg
     4861 gcgcttccag accgtgatgg tcgatccccc ctcacccggc gatacaatca agattctgga
     4921 ggggttaaag tcgcgctacg aagatcacca caaagtccgc atcaccgacg aggcgattgc
     4981 agcctcggtg aaattgtccg accgctacat ttcgggaaaa tttcagcccg acaaagccat
     5041 tgacgtcctg gatgaagccg gctcccgggc ccacctggcc tctttcacca aacccgtcac
     5101 tttcaccgac atcgaacagg aaatcgcccg gatccaacgg gacaaagagc attcggtgaa
     5161 aaaccaggag ttcgaacgcg ccgcccagct gcgggacgag ttgaagttca aaaaagacga
     5221 attggagaag gcccgccggg cctgggagga agagcgcagc aagcaggtgg tggaactgaa
     5281 ggccgaagag atcgcccagg tggtttcgaa gatgaccggc atcccggtct tccggttaga
     5341 agaggttgag tccaagaaac ttttgcggat ggaagaggag ttgaccaagc gggtggtggg
     5401 ccagagcgaa gccattaacg tcatctccaa agcgatccgc cgctcccggg ccggattgtc
     5461 cgacccgcgc cgtcccatcg gctccttcat cttcctgggc cccacaggct gcggtaaaac
     5521 cgaactcgcc cgcgccctgg cggccttcct tttcgaagac gaagaggctc tggtccgcat
     5581 cgatatgtcg gagtacatgg agaagttctc cgtctcgcgc ttgatcggcg cgccgcccgg
     5641 ctacgtcggc tacgaagagg gggggcaact gaccgaaaaa gtccggcgca agccttactc
     5701 cgtggtgctt ttggacgaaa tcgaaaaggc ccacccggag gtcttcaacc tcctgttgca
     5761 ggtgttggac gacggcaccc tgaccgactc cttcggccgc aaggtcgatt tcaagaatac
     5821 cgtggtcatt atgacctcca acatcggcgc ccgcgagatc cacgccggca agcatctggg
     5881 cttcgtcccc gaagaggagc aattctccta cgaaaggatg cggcagaaga tcaccgaaga
     5941 ggtcaaaaaa atattcaacc cggaattttt gaaccggatt gacgatatgg tggttttcca
     6001 cccgctgggg cggccggaga ttttgaagat cgtcgacatc cagttaaagg aagttgccca
     6061 gcgcctggcc gagaaggaag tctccttcac cctcaccgac gccgccaagg aattcttagc
     6121 cgacaagggg ttcgacccgg tcttcggcgc ccggcccctc aagcgggcga tacaaaaata
     6181 cctggaagac ccgatggccg aagagatctt gaaaggccag ttcggtacgg aatgcgaagt
     6241 gttagtggac aaaggcgaag gcgacgggct gcgcttcacc atcgctggcg gccgccccac
     6301 tctcgtgccg gtaaagaagt aagagattat tcggtttggg aaaattgtag gggcggggtc
     6361 accccgcccc tttttatttt ccctctcctc gtaggagaga gcaggtcgaa gaccccgcca
     6421 gaggcggggg aggggtcagc cctttgttgc ttttcgcttc gcataggggc gaccgccccc
     6481 ggtcgcctat aattttccgt agccgaggat tttatcctcg gtgccccgct ggcggggcat
     6541 tgatttgatt ttccctctcc ttcgagggga ggggtacagg gagaggtcat tcttggtaga
     6601 ccggctcgtc tctgagccgg tcgagacgga tgtgtagggg cgaatcacaa ttcgcccaag
     6661 atttttccct ctcctgtagg agagggtagg gtgaggtcaa gatgtagggg cgaacctcgt
     6721 gttcgcccat ctctcagagg ttcttcttct ccctctcctg tagtggttcg gcccgtacgg
     6781 gctcaccacc ctgtcttcga ccctgagggt ctctcgttca gagcctgacg gctcttcaga
     6841 gccgaaggca gacccgatgg gagcttgccg aaggggagga ggtcggggtg aggtcaaatc
     6901 cgtagagctg attcacgaat tgcccctacc cacaatccgt ttaatctcgc cagagtcgca
     6961 tcgaccgctc caaaatccgt tgtcccatac gggtcgcacc gacagaattt tggggtcaca
     7021 tcgggggcac ataggtgccc cttcccccgc taatttgggg ttgccttggg ggtaaaacgg
     7081 acaaagtgcg aaataatggg caaccttcac cccggtgcga ggcgaaggtg atttttccaa
     7141 atctgaattt tttagtacgt ccgcaccggg cgggagcgga aggaggccag gtaggtcttc
     7201 aggtcttcgg cgatttcgcc cgcccgctgg tagcggtaga ggacgctttt ctccagcgcc
     7261 cgcaggatga tccggtcgaa cagttcgggc accttgtcat tgatctgtga gggcggcggc
     7321 ggcgtgaggt tgatgatgtt gtagattaaa gcggaaaggc tttcgccggt gaagggtttc
     7381 ttccgggtca aaaattcgta catcaccacc cccagagcaa agatgtcggc ccggcggtcg
     7441 atctcctgac cggtcaactg ctccggggaa atgtagttgg gcgtgcccaa ggctacgccc
     7501 gactgggtca tattggaggt cgctatgcgc gcgatgccga agtccatcac cttgatgttg
     7561 aaaccgttta ggaccatgat gttggccggc ttgatgtcgc ggtggacgat cccctggtcg
     7621 tgggcgtact ccagggcatt gcagatctgc gtgatgatcg aggccacgat gcggaagttc
     7681 agctcgactt tctttttgat cacttcctcc agcgtgtacc cctgcaggta ctccatggcg
     7741 atgtactgaa tgtcgccgtc ctgacccacg tcgtagacag tgacgatgtt cgggtgggag
     7801 agcttgcccg cggctttggc ctcgcgtaaa agccgttcgc gcagttcggt cacctcgtcc
     7861 gggctagagg caaaatccat gcggatcgtt ttgagcgcca ccagacggtc cagcgccgga
     7921 tcgagtccct tgtagaccgt acccatggcg cctttgccca aaacctgcag cacctggtag
     7981 cgtccgagtt tgcccagttg ccctgcttcg gcctgctttt tggcttcggc cttggcctcg
     8041 actaacattt gcgtctcttc ggccgccatc tgctccggag ttttcatctc cagttccacc
     8101 gtggcgaggt cggaagagac catgtgctgg gtggcgtgca tttcagccag ttcaagggaa
     8161 gccggtccat attcgacccg cgcccgttcc ggtcggggcg gctcggcctc ttcctgccgt
     8221 gcgaaccttt ggtactggcg cggtgcgtac ccttcctctt ccccagtctc gacctcggcc
     8281 tgggcacctt ctttcttggg tttgatcgta aaactggcgg cgagaaagaa gaacacttcc
     8341 atcatcgggt aaaaaacctt gggcattaag ttaaaggagg cgaacagaat gaagttgaag
     8401 ttgagaatgg caaaggcgaa caccgccaga atgaccgccc ggtagaggag ggccaccttg
     8461 ggcaggatca gcgcggagaa gacgccgatg aggatcaaaa tacctacgtc caagagcgtg
     8521 gagagggaga gactgctgta gaaccgccgg tgaatgatgt tttcaatgac cgtggccgtg
     8581 acttccaccc gcgggatgcc gcgcgcgatg gcggttttgt actgctgggt caggccggtg
     8641 gcggtcatcc cgaccacggc gaccttgtcg cgcaaagcgg aggggtcaac ccgtcccgag
     8701 agcacgtccg ccgcggaaac ggaggggaag gtattcgccg gtccccgaaa ggcgatgagc
     8761 atctgtccgt gatcgtcaat cggaatcgac aaattatcca gccagatttt tttgcccgtc
     8821 tcaattttca cctgctggcg gaccagcccg tagtacgccc gggccgcctg caccgccagg
     8881 gaagggtagt agaagccttc gtacttgacg atgagcggcg cccagcgcag catgccgtcc
     8941 gggtcggggg cgaaagccgt gtgccccagg ccaaaagcgg cgctgtccaa aggcgcttcg
     9001 gggtagacga tttcccgcgc caagcggggc ggattgtcct gcagatcgaa ctcttcacca
     9061 tagatgaggt aggcggagct ggtaatgtac gccggcgtgg tgatcgtatt gacccccgtc
     9121 tccgcaaggg tgaaactgac cggcaggatg acgttctttg cccgtttgat gacgtccgct
     9181 aaactgtcgg taaccgcacc ggtgtccggc gccgtgaaag gcaaagggaa attaccggcg
     9241 atcactttgg gcttttgggc cgagagcgca tcgaggaagc gggccatcgt cgctttgggc
     9301 cagggccagt ggccgatctg gttaatggcc tggtcgtcca cggccaccac cacgacatct
     9361 ttgccgggat ccgacttgcc ccggaacttg agcattaagt cctggatttt gacctcgtac
     9421 ttggccagtg agtcgtggtg gtccaggtag agcgtcagca ccaggagagt gatgaggata
     9481 tagaggaggt tgccggaaaa ttttttcagc atgcgtttga tcctgccggt ttcatttcct
     9541 tacgagtaca acaatcccac aggtacggcc caattcagag caggcttacc gtcccgtggc
     9601 tatccgctca atgagaaaag ttggcacctg gaattcggcc atcttctgct gcaccttctg
     9661 gaaatcgtac gccacgcgct tgagggtggc ctggtgcctc tcggtatcat agactaagta
     9721 actggctttc ggattcccgt cccgcggctg gcccaccgaa ccgatgttga tcaggtagcg
     9781 gctgccgccc tcgattttca ccccggtgtc cgccaaaagc gtacagggct gggaaccggt
     9841 ttttttgacc agcgccggac ggtgcgtgtg cccgatgaga cagaccggct cccgaaaaaa
     9901 agggaagcag tgttcggcgt cctccagcgt gaggacgtag tcccagttga gcggctcgtg
     9961 cggggaggcg tgcaccaggt agcagtcggc cacccggtgg tcgagctgaa aaccggagag
    10021 gatttcctga gcccgctcgt ctaagacccg ccgggtccag tcgagcgtct gcttggcgta
    10081 gtggttgaag aggtcgagtt ccagaagtcc cagggcggcg tagtcgtggt tgcccatgag
    10141 cgagacctcc gcagcttcgg ccaccaaacg cacgcactca ttcggcgacg ggccgtaccc
    10201 cactgcgtct cccagaaaaa aaagtttctc caatttctcc gccgcgcagt cggccagcac
    10261 cgcttccagt gcctcgaggt tgccgtgcac gtcggagaag aaccccaact tcatcggctg
    10321 gagccttcta cgaagcggaa gaccgagcgg ccgatctcca gtaaatcgtc attgcgcagc
    10381 cccacccgct ccaccggttc gccgttcacc ttcacccgcc cgcggcggcc gacgttcacc
    10441 agcacgaatc ggctgtcctc gcggattaac ttggcctgga tgccggaaac ccagaaccct
    10501 ttcacctgga tgttgacgaa agacgacttg ccgaaggtca ccacgtcgcg gtccagcttg
    10561 aactgctcgg cttcgcagtg ggccactccc tgcaacaccg gcccgccgta cttggtgact
    10621 atctgccggt cgcgcaggtc tttttccaaa agctcccgct gttttttggt cttcagcacc
    10681 atcgtgccgt ccatgtcggt gggcttgtag tcgtgcccgg ccgccacgtg gtagaagagc
    10741 tggtacttgc cgatggtgat gacgtcgtta tgcttcagaa tttcttcggc caccttgcgg
    10801 ttgttcacaa aggtgccgtt caagctctcg ttgtccagga tcaccgcggt gttgccgttg
    10861 aactcgatct gggcgtgctt gcgcgacacg ccgcggttgt ccaggacgat gtcgttgtcc
    10921 gacgtccgcc cgatggagat ccgcttcttc tcggttacca cccgttcaat aattttgtcc
    10981 tcatatttga cgaggatttc aggcataggc gctccttttc cgccgcagag aatcccccac
    11041 acttgcccgt ctggtcatct ctcctatatt gtcggtcggg tcgagggaaa cttaagagtg
    11101 gccggtcgct acggcgctgt gccgaagaaa gttgtcggtt gcgcaagatc tgcgcagcac
    11161 cggaagacgg taccccggcc cgaaacagtt tccgcacccg cccgagccga tgttggtttc
    11221 gtttccgtgg aacgtatgca tccagagact ctagcccggc tcctgcgcca gatccggacg
    11281 agaaaaatat ctccgccgca agctctcgaa cggttgaaac tcttaccctt tgaggatctc
    11341 ggctttgcga ctattgacca ccaccgccac ctgcgccagg gactcccgga agtgattctg
    11401 ggcacaggca aaaccgcccc gcaggtggag aagatcgccg aacggctcct ggcggccgga
    11461 gcgccggtcc tggccacgcg gttggatgaa ccggtacgga agatcctccg cgagcacttc
    11521 ccctgggcac gggagaacgc cttagcgcgg gcagtcttca tatcctcaac gaaacttgcg
    11581 aagaaaactc gggggggcgg acgtttattg ataatttctg ccggtacggc tgaccagccg
    11641 gtggccgaag aggccagctt tgccggcgag gtgatcggcc tgcggaccaa acgggcgttt
    11701 gacgtcggcg tggccggtct ccaccgtttc tttgcgcaca ctgactcgtt gcggtgggcg
    11761 gatgtgataa ttgtcgtggc tgggatggaa ggcgccctgc cctcggtcgt cggcggcgtg
    11821 gtgggtaaac cggtgattgc agtgccgacg tcagtcgggt acggcaccag tttcggcggc
    11881 ttgaccgcgc ttttcgccat gctcaactcc tgcgcggcgg gcgtcacggt agtgaacatc
    11941 gacaacggct tcggcgccgc cttcgccgcc tggcgcatat tgcaagtgaa gccgaaatga
    12001 aaatcgctta ctgcgactgc atcggcggcg ccgccggcga tatgctcctg ggggcactgg
    12061 tcgccagcgg cctgaaactc gaggcactcc agaaggaaat tgcgaagtta tctcttccag
    12121 aggtcagggt cacggtgcat ccggtgaaac ggcaggcgct ggcggccgcg cagattgaaa
    12181 tctcagcgcc ggaagcccaa gcgtaccgcc atttgaagga aatagagcaa attatttcca
    12241 cgagcggact ggccgacgcg gtcaaactga agagtctggc catcttccac cgcttggccg
    12301 aggccgaagc ccgcgtgcat ggaagttcgg tggaggaaat tcacttccac gagttaggtg
    12361 cggtggacac gatcgtggac gttgtcggca gcgtcgccgg cttcgaaacg ttgggaatag
    12421 aaaaaatcta ctcttcccct ttgcctctgg ggcggggaat gattgcctca gagcacgggc
    12481 ctctgccctt accggccccg gccacagccg aactggtacg gaattacccc gtgcgcccgg
    12541 tggacattga aggggaactg gtcactccca ccggagcggc cattctcacc actttggcag
    12601 agttcacggc cccaccgccg tatcggcttt tgaccgtcgg ctacggcgct ggctcccgga
    12661 atctcgccaa tattcccaac gtcctgcggt tgttcatcgg ggagacggta ccggcacccg
    12721 cagtcgacac ggtgacgcag atcgagacca atctcgacaa caccagtccg gaagtcctgg
    12781 gctatctggc cgaacggctt ttttctgcgg gggcacttga cgtatacttc gttccggtga
    12841 cgatgaaaaa gagccgcccc ggcgtcttgc tcggcctcct ggctaacccg gaagatcggg
    12901 agaagttcgc cgccatcctc ttcgaagaaa ccggaaccat cggcctgcgc tattgggaat
    12961 gtcaaaggcg gaagttgccg cgcagcgagg aggtggtc
//
LOCUS       DBNGZD010000070         6928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_633906, whole genome shotgun sequence.
ACCESSION   DBNGZD010000070 DBNGZD010000000
VERSION     DBNGZD010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_633906"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16GM_01885"
     CDS             <1..186
                     /locus_tag="AC16GM_01885"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR2922454.1"
                     /translation="LSAEELKELAARAIEEVGAKTQSDVGKVMKVLMPQVRGKADGKT
                     VNACVQALLEATPSPST"
     gene            289..939
                     /locus_tag="AC16GM_01890"
     CDS             289..939
                     /locus_tag="AC16GM_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR2922455.1"
                     /translation="MNWVDLVVLLLVIGGAAYGAKRGLIREVMSVLGIVVGIVLAIHH
                     HDAVAMTFLSKMKASPLFVSFLSFLIVLGVSYLGFKILGFALARAASVNQLGNADKVG
                     GAIVGATKAWIIVGFIFLLLFYLPLPDSMLKAVDSSFIAPAMLGTMPVLYEGTKMFHP
                     SGANFLAKLKAPLTRASNKKAEQIQGGMLLPGDWQNDRTEKCLERLDAHFAVKPQQ"
     gene            complement(1038..1790)
                     /locus_tag="AC16GM_01895"
     CDS             complement(1038..1790)
                     /locus_tag="AC16GM_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922456.1"
                     /translation="MTFVQYIPIFTTLFSIFFSWRIFRHYRERKSGPHLLWWGAGIGI
                     YGLGTLAESLVTLFGWHAGLFRLWYITGALLGGAPLAQGTVYLLLKRTTANRLTAVIV
                     PYFLFAATCLLLAPLQNQPADVRILTGKVIGLPDGSWSWVRFLSPFMNTYAVIFLIGG
                     AILSAFRYKKDPTMHHRFVGNVFIAVGAILPGVGGAFSRMGHTEVLYVTEFIGLLLIW
                     RGYRLCVKKQEGAEPSLDVRRAVKELLPQEKT"
     gene            complement(2146..4356)
                     /locus_tag="AC16GM_01900"
     CDS             complement(2146..4356)
                     /locus_tag="AC16GM_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR2922457.1"
                     /translation="MERMRPGVLVLTFMFLFSSLFLLSSVPARGENAALPAKGDESNS
                     PDSARMPVYQLDGIVVTANRYEKSAFAVPYAMSILSADKFQQAGAPIIADLLRNLAGV
                     DVTDAGPFRTRPIIRGLAGSRILVLVDGQRMNDTRENTFSGAELSQVSPGQVERVEVL
                     RGSNSVLYGSNAMGGIINILTRQPSARRGPLAVSGSLVNRYSTTDQQPYSRLNLNLAN
                     HRWRLAGGAEYRRANNYHAPGRSVFGRGREIQNSNLRRSVGLDFAGSYRLADRHTLAL
                     EAQSMLNSEIGFPETPNPTFPVGIFFPYHDRSKIALTYEGKKITPRLASIRATLYGQK
                     NKKDLRTNISLPNVPFPGAALYDTSHTYTTVLTVGANLQQIYTLSGKQTFSWGADYYR
                     EMTDGLTRDFTHITSPFFSSNTMKQSASVPENHLDALGLYLQDDVQPFERFSGHLGAR
                     FDWYRQVTDYTDGYNDIATGRPIAPQKKNQSALSGSAGVQYQVIPEVRLSGNVGTAFR
                     IPNLVEKFFSGTQDQQLVIPNPDLDPEKNISADLGVKFRFDRFSGSVTAFHNQLRDFI
                     ELRATGDSVTKSGRRQAVWHYENISKVRFQGVEAELEAKLPRGFFSYGNASYQRGDNL
                     STDRALYVAPAKVVLGLGWKAPQGQFEAEVNDRLILDQKRVDPDPSAVFQLPTPGFDL
                     LSLQTSYTWRWWTFNFNIHNLTNRKYSEPLNAASPFNPITEPGRNFIFGLTTRF"
     gene            4587..>6928
                     /locus_tag="AC16GM_01905"
     CDS             4587..>6928
                     /locus_tag="AC16GM_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR2922458.1"
                     /translation="MDRHSLEVLEFDKIVALVGERCLAAPGRELAGALTPQTDPGRIA
                     TWLSETAEVKEIITTDQHFPLRSYPDIREHLATVKMEGTFLEPLRLLEVADFSGLVGD
                     LVRTAKALLNAYPLVTSHLAGLTAHSELKKAIEKAIDPSGEIRDSASPALHTLRQQKQ
                     KIRREILAKLEAILSRRKTPQAQVTLRDNRYVIPVDEHDLSRQKGIVHDRSASGATFF
                     VEPLETVELNNRLRETEIKERQEIDRILRALSDHVRKHQDELAENLEILAQLDFLRAK
                     ARLSVDLNGSAPKITAGAFVSLLNARHPLLLLQAKTQPGMQVIPLDFSLGRDFSCVIV
                     TGPNTGGKTVALKTLGILVLMAQAGLHVPASPDSEIGSFGSVWADIGDEQSIEQSLST
                     FSSHMSRIIQALSRADAATLVLFDELGVGTDPKEGAALGEAVLLDLLEKKAKIVATTH
                     YSALKTLAQQYPQIRNASLEFDRKTLRPTYRFSLGLPGGSYALEIAERLGMPKKVTNK
                     AAELVGSQERSLVKLLEEVEENLHALSEDRKQVALQKATVGELERFIAEKKKKLDERE
                     RQAKAAALEEAQEIVRTTRKETERLVAEIRRSQADKKVVKETQTSLQRTAEELAAKGK
                     ELAPVVTPEMTELRIGDTVSVALSTDGAQQISGEVVELANDERKVRLWVGNIFYTVYR
                     EQIVKKVEPSAVPSSSTPPRRQLEEEKGVSRELSVRGMTVDEALAEIDKYLDGVALSG
                     LSEVVIIHGKGTGTLRKEVGAYLQNHPLVAATRLGQWNEGG"
BASE COUNT         1499 a         1948 c         2141 g         1340 t
ORIGIN      
        1 ctgtcagcgg aagaattaaa ggagttggcc gccagagcca tagaggaggt gggagcaaag
       61 acacagtcgg acgtcggtaa ggttatgaaa gtgctgatgc cgcaggtgcg cggcaaggcc
      121 gacggcaaaa cggtcaacgc ctgcgtgcag gcgcttctgg aggccacgcc aagtcctagc
      181 acatagaccg accatcgtgc gccggccagt gagggtgaaa catttcacgt tttgaccgat
      241 acgctaagta gaggaacagg acgtgaactt ttcatcggag gaggggaaat gaactgggtc
      301 gatctggtgg tgctattgct ggttatcggc ggcgccgcct acggagccaa gcgggggctc
      361 atccgggaag tcatgagcgt tctcggtatc gtggtgggaa ttgttctcgc cattcaccac
      421 cacgacgcag tggcgatgac ctttctctcc aaaatgaagg cctcgccgct tttcgtttcg
      481 tttttgagtt tcctcatcgt gctcggagtt tcctacctcg gctttaagat tttaggattc
      541 gctttagcgc gggccgcctc ggtgaatcaa ctggggaacg ccgataaggt cggcggcgcc
      601 atcgtcggcg cgaccaaggc ctggatcatc gtcggcttca ttttcctgct tttgttctac
      661 ctgcccctgc cggatagcat gctgaaagcg gtggacagct ccttcatcgc cccggcaatg
      721 ctgggcacga tgccggtgct ctacgaaggc acgaagatgt tccaccccag cggggcgaat
      781 ttcctggcca agttgaaagc tccgctcacg cgggcgtcga acaagaaagc cgagcagatt
      841 cagggcggga tgcttttgcc gggagactgg cagaacgacc gcaccgaaaa atgcctggag
      901 cgcttggacg cgcacttcgc cgtcaagccg cagcagtaga gactccctgg ccttaacgta
      961 ttaaaggaat cgtagggcgg cccgtacggg ccgcctttct ttttttcctc cccttgaggg
     1021 gaggattaaa ggaggggtca ggttttttct tgcggcaata gttcttttac tgcccggcgg
     1081 acatcgagag aaggttcggc tccttcctgc ttcttcacgc acagccggta tccccgccag
     1141 atcaaaagca gcccgatgaa ctcggtgacg tagagcactt cggtgtgccc catacgggaa
     1201 aaagcgcccc cgaccccggg caggatggcg ccgaccgcaa tgaagacatt accgacgaag
     1261 cggtggtgca tcgtcgggtc tttcttatag cgaaaagcgg agaggatggc gccgccgatg
     1321 aggaagatca cggcataggt attcatgaac gggcttaaga agcgcaccca ggaccagctg
     1381 ccgtccggca gaccgatgac cttgccggtc aggatgcgca cgtctgccgg ttgattctgc
     1441 agcggggcca ggagcaggca ggtggcggca aacaagaaat agggaacgat gaccgccgtc
     1501 agccgattcg cggtcgtccg cttgagcaga aggtagaccg tcccctgcgc caaaggcgcg
     1561 ccgcccaaaa gcgctccggt gatgtaccag aggcggaaga gtcccgcatg ccacccgaag
     1621 agagtaacta aactttccgc cagcgttccc aacccgtaga tgccgatgcc cgcgccccac
     1681 cagaggaggt gcggcccgct ctttcgctct cgatagtgcc ggaaaatccg ccaggagaag
     1741 aaaatcgaga aaagcgtcgt gaaaattgga atgtactgga caaacgtcat agttcgatca
     1801 ctttccgctt aatctcaact tgtcacccgg gcgaccgcgg gcgtggattc tgtattaggc
     1861 tgtagtcgag acttacatga cctcaccccg accctctcct tcaggagagg gggtaaattt
     1921 ccctcccctt tgaggggagg gtgagggagg ggtcaagagg tcaccaacca aagatctctc
     1981 ttggtgaggc ggcgagggcg ccgcgcccac ggcagtgaaa tggaacgccg cgtggggcga
     2041 ccgctctcgg tcgcccggaa cggagatagg tagaccggtt tgtccctgag ccggtcgaaa
     2101 attgcggcgg ttcaactcgt cgaaaaccac aacaggaaat gcctctcaaa accgcgtcgt
     2161 caaaccgaag atgaaattcc ttcccggctc ggttatcggg ttgaaggggc tggccgcgtt
     2221 caagggctcg ctgtatttcc ggttggtcag gttgtggatg ttgaaattga acgtccacca
     2281 gcgccaggtg taactcgtct ggagggaaag gaggtcaaat cccggcgtcg gcaactggaa
     2341 gaccgcggaa ggatcggggt ccacccgctt ctggtcgaga atcaaccgat cgttcacttc
     2401 ggcctcgaac tgtccctggg gcgccttcca gcccaggccg aggacaacct tggccggcgc
     2461 cacatagagc gcccggtcgg tggagaggtt gtcgccccgc tggtaggacg cgtttccgta
     2521 actgaagaat ccccgtggca gcttggcctc cagttccgct tccacgccct gaaagcgcac
     2581 tttgctgatg ttctcatagt gccagaccgc ctgcctgcgg ccgctcttgg tcaccgaatc
     2641 gccggtggcg cgcagttcga tgaaatcgcg cagctggttg tggaaggcgg tgaccgagcc
     2701 ggagaaacgg tcgaagcgga atttcacccc cagatcggcg ctgatattct tctcggggtc
     2761 taagtccggg ttggggatga ccaactgttg atcctgggtt ccggagaaga acttctccac
     2821 gaggttggga atgcggaagg cggtgcccac gttcccgctc aagcgcactt ccggaatcac
     2881 ctggtactgc acacccgcac tgccgctgag ggccgattga tttttcttct gcggtgctat
     2941 cggccggccg gttgcgatgt cattgtaccc gtcggtgtag tcggtgacct gccggtacca
     3001 gtcaaagcgg gcgcccaggt ggccggagaa gcgctcgaag ggctgaacgt catcctgcag
     3061 gtagagaccc agggcatcca gatgattctc cggcaccgat gccgattgct tcatcgtgtt
     3121 cgatgagaag aaaggcgagg tgatgtgagt gaagtcgcgg gtaagaccgt cggtcatttc
     3181 gcggtagtaa tcggcacccc acgaaaaggt ctgcttgccg gacagagtgt aaatttgttg
     3241 caaattggcg ccgacagtca ggacggtcgt gtaggtgtga ctcgtgtcgt acagggcggc
     3301 gccgggaaag ggaacgttgg ggagggagat gttcgtccgc aagtctttct tattcttttg
     3361 gccgtagagc gtggcgcgga tggaggccag acgcggggta atcttcttcc cttcgtacgt
     3421 cagggcgatt tttgaccggt catgataggg gaagaagata cccaccggaa aagtcggatt
     3481 gggcgtttcc gggaaaccga tctccgagtt cagcatgctc tgcgcttcca gcgcgagcgt
     3541 gtgccggtcc gccagccggt aactgccggc aaaatccagc cccaccgagc ggcgcagatt
     3601 tgaattttga atttcgcggc cccggccgaa aacgcttcgg cccggtgcat ggtagttatt
     3661 ggcgcggcgg tactcggcgc cgcccgccaa gcgccagcgg tggttggcga ggttgagatt
     3721 cagccgggag tagggctgct ggtcggtggt gctgtaccgg ttgaccagac tcccggacac
     3781 cgcaagcggc ccccgccgcg ccgacggctg ccgggtgaga atattgatga tcccgcccat
     3841 ggcgttggag ccgtagagga ccgagttcga cccgcgcaaa acctccaccc gctcgacctg
     3901 acccgggctc acctgcgaga gttccgcgcc ggaaaaagtg ttctcgcggg tgtcattcat
     3961 gcgctgaccg tccaccagca ccaggatgcg gctgcccgcc agcccccgga tgatgggccg
     4021 ggtgcggaac ggtccggcat cggttacatc gacaccggcc aggttgcgca agaggtcggc
     4081 gatgatcggc gccccggcct gctgaaattt atcagccgag aggatcgaca tcgcgtaggg
     4141 gacggcaaaa gccgatttct cgtaccggtt ggcggtgacg acgatgccgt ctaactggta
     4201 gaccggcatt cgggcgctgt ccggggaatt actttcgtct ccctttgccg gcagtgcagc
     4261 gttttccccc cgggcgggga cactggagag cagaaaaagt gaagagaaaa ggaacatgaa
     4321 cgtgagtacc aacacacccg gtcgcatccg ttccatcaga acctccttta agacgcctct
     4381 cgcccgggcc acttccgggg ccgggcactt accgataacg tttcgcaaca atatggatcg
     4441 ggcgaaattc gtgctatgac gcttatcata aaccgaaggg aagcattccg gaccgctgca
     4501 gccagaagga ggtacggttt tgcaaccggc ggcttatatt acttaccggt tactctaagc
     4561 acaggcgata gatcataaag aggtgtatgg atcggcactc ccttgaggtt ctcgaattcg
     4621 ataaaatcgt cgccttggtc ggcgaacgct gtttggcggc tccgggacgc gaactggcgg
     4681 gagcgctcac gccccagacc gatcccggaa gaatcgcgac gtggctgtcc gaaacggccg
     4741 aggtaaaaga aatcattacc accgaccagc atttccccct gcgatcgtat ccggacattc
     4801 gggagcatct ggctacggtg aaaatggagg gaacgtttct ggagccgttg cggctcctgg
     4861 aggtggccga tttttcgggt ctcgtgggtg acctggtccg cactgcaaag gccttgctta
     4921 acgcttatcc tttggtaacg agccatctcg ccgggctcac tgcccattcc gaattgaaaa
     4981 aggcaatcga aaaagccatt gacccctcag gcgaaattag agattccgcc tcgcccgctc
     5041 tgcacaccct gcgtcagcag aagcagaaaa tccggcgtga gatattagcc aaacttgaag
     5101 ccattctgtc ccggcgcaaa acgccacagg cacaggtcac cctgcgtgac aaccgctacg
     5161 tcatcccggt agacgaacac gacctctcgc gtcaaaaggg tatcgtgcac gaccgctcgg
     5221 cctccggggc gacttttttt gtcgagccgc tggagacggt ggaactgaat aaccggctgc
     5281 gcgagacaga aatcaaagag cgccaggaga tcgaccgcat cctgcgggcg ctctccgatc
     5341 acgtccgcaa gcaccaggac gaattggccg aaaatctgga aattctggcg caattggatt
     5401 tcctccgggc caaggcccgc ctgtcagtcg atttgaacgg ctcggcgccg aagatcaccg
     5461 ccggggcctt tgtttctctc ctgaacgccc ggcatccgct tttacttttg caggcgaaaa
     5521 cgcagcccgg aatgcaggtc ataccgcttg atttttccct gggtcgggat ttttcctgcg
     5581 tgatcgtcac cggcccgaat acggggggaa aaaccgtggc cctgaagaca ctgggaattc
     5641 tcgtgctcat ggcgcaggcc ggcctgcacg ttcccgccag ccccgattcg gaaatcggct
     5701 ccttcggcag cgtctgggct gacatcggcg acgagcagtc gattgaacaa tcactctcca
     5761 ccttctcctc tcatatgagc cggatcatcc aggcgctttc gcgggccgat gccgctacac
     5821 tcgtcctctt cgacgaactg ggcgtcggaa ccgacccgaa agagggtgcg gccctgggtg
     5881 aggcagtgct cctcgatttg ctggagaaaa aagccaagat cgtggccacg acccactatt
     5941 cagctttgaa aactctggcc cagcagtacc cgcagatccg caacgcttct ttagagttcg
     6001 atcggaaaac gttgcggcca acgtaccgtt tctcgctcgg cctgccggga ggcagttacg
     6061 ccctcgaaat tgccgagcgg ttggggatgc ccaaaaaggt cacgaacaaa gccgcagaac
     6121 tggtggggag ccaggagcgg agtctggtca aactcctgga agaagtggaa gaaaatctcc
     6181 acgcgctctc ggaagacagg aaacaggtgg cgctccagaa agcgactgtg ggagaactgg
     6241 agcggttcat tgcggaaaag aaaaagaaat tggacgagcg cgaaaggcag gccaaagcgg
     6301 cggcacttga ggaagcgcag gaaattgtac gtacgacccg caaggagacg gaacgtctgg
     6361 tagcggagat ccgccgctcg caggcggaca aaaaagtggt gaaagaaacg caaacgtcgc
     6421 ttcaacggac cgcagaagaa ctggcggcga agggaaaaga gttggcaccg gttgtgactc
     6481 cagaaatgac cgaattgcgc atcggcgaca ccgtgtcggt tgccctgtct acggatggtg
     6541 cccagcagat ttccggcgaa gttgtggaac tggccaacga cgagcgaaaa gtacggctgt
     6601 gggtcggcaa catcttctac acggtctacc gggagcagat cgtgaagaaa gtcgaaccgt
     6661 cggcagttcc gtcatcgtca acgccccccc gccggcaatt ggaagaagaa aaaggtgtct
     6721 cgcgggagct ctccgtgcgc gggatgaccg ttgacgaagc gctggccgag atcgacaagt
     6781 acctcgacgg cgttgccttg tccggactct ccgaagtggt tatcattcac gggaaaggga
     6841 ccgggacgtt gcgcaaagaa gtgggtgcat acttgcagaa tcatccgctt gtagccgcca
     6901 cccggctggg gcagtggaac gagggggg
//
LOCUS       DBNGZD010000071         1651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1398924, whole genome shotgun sequence.
ACCESSION   DBNGZD010000071 DBNGZD010000000
VERSION     DBNGZD010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1398924"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /gene="recR"
                     /locus_tag="AC16GM_01910"
     CDS             complement(<1..544)
                     /gene="recR"
                     /locus_tag="AC16GM_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR2922459.1"
                     /translation="MPTSTALENLITRLTKLPGIGRKSAQRVAFYLLKRPKEEAFLLA
                     EALRDVKEKVRTCSVCANITEDDPCPICRDPGRDRSTICVVEEINDVAAFDRTGEYRG
                     LFHVLGGALSPLDGIGPDELKIKELLTRISGGVKEVIVATNPNVEGEATAVYLAKVLK
                     PLGIKVTRIARGVPVGGALEF"
     gene            complement(583..894)
                     /locus_tag="AC16GM_01915"
     CDS             complement(583..894)
                     /locus_tag="AC16GM_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR2922460.1"
                     /translation="MKGLGDIMKMAQNMQAKMETVQQELAVVRVEGTAGGGMVRVVAN
                     GKQEIMEVKIDPEVFNANDREMLEDMILAASNQAHQRAQELAAEKMAQLTGGIKIPGL
                     F"
     gene            complement(928..>1651)
                     /locus_tag="AC16GM_01920"
     CDS             complement(928..>1651)
                     /locus_tag="AC16GM_01920"
                     /inference="COORDINATES: protein motif:HMM:NF044669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922461.1"
                     /translation="LRMLSSLGALKTEFDKLSQPRIFLELELVKLARLDRTVEIANLI
                     RRLEGGSMGGRHPTNAPLELLLGETTEQNPPRRSGSPTANTSVPVKTSSALEKEVATA
                     IPVASVKNFILDDIRRGWPTFVEKLRAEKPTLAAILYEGKAAQVEGNIVTVEVPNGQK
                     FHLQMLEKRENLRLVEEILSTACGIPPEAGLRVKISLAEKAAAEVSEKPSAGKRGEEI
                     PEVKQLIEKFDAEVIDRRPVRK"
BASE COUNT          288 a          523 c          441 g          399 t
ORIGIN      
        1 cgaactccaa ggcacccccc accggcaccc cccgggcgat gcgggtgacc ttgatcccca
       61 ggggcttgag caccttggcc aggtagactg ccgtggcctc accctctacg ttcggattag
      121 tggcgacgat gacctctttg acgcctcccg aaatccgggt caaaagctct ttaatcttca
      181 gctcatccgg gccaatgcca tccagaggcg agagcgcgcc gcccagaacg tgaaacagac
      241 cccggtactc gccggtacgg tcaaaggcgg ctacgtcatt gatttcttcg acgacgcaaa
      301 tggtgctccg gtcacgtccg gggtcccggc agatggggca ggggtcgtct tcggtgatgt
      361 tggcgcagac cgagcaggtg cgcaccttct cttttacgtc gcggagcgcc tccgccagaa
      421 ggaacgcttc ttctttcggc cgtttgagga ggtaaaaggc gacccgctgg gcgctcttgc
      481 gcccgatgcc cggcagtttc gttaaacggg tgatgagatt ttcaagagca gtggaagtag
      541 gcattttgct tttactggct actggtccct ggctactgtc ttttagaata gccccggtat
      601 tttgatgccg ccggtcaact gcgccatctt ctcggcggct aattcctgcg cccgctggtg
      661 cgcctggttg gaggccgcca gaatcatatc ctcgagcatt tcccggtcgt tggcgttgaa
      721 cacttccggg tcgattttca cttccataat ttcctgtttg ccgttagcca ccacccgcac
      781 catccccccg ccggcagtgc cctccacccg cacaaccgct aattcctgct gcaccgtttc
      841 catcttggcc tgcatgttct gggccatctt cattatgtcg ccgagacctt tcaccggact
      901 gctccttgag agagttgcgg gcgaccgcta tttgcgtacc ggccgccggt caattacttc
      961 cgcgtcgaat ttttcgatca actgcttcac ttccgggatt tcttcgcccc gcttccctgc
     1021 tgatggcttt tcgctcacct ccgccgccgc cttttcggcc agagagattt tgacccggag
     1081 cccggcctca ggcggaatgc cgcaggcggt ggagagaatc tcttccacca gccgcaggtt
     1141 ttcccgcttc tccaacatct ggaggtggaa cttttggccg ttgggaacct ccacggtcac
     1201 gatgtttcct tcgacctgcg ctgccttgcc ttcgtacaga atcgccgcca gagtcggttt
     1261 ctctgcgcgt aacttttcca cgaatgtcgg ccagccccgc cggatgtcgt caaggataaa
     1321 attcttcacg cttgcaaccg ggatcgccgt tgcaacctct ttctccaggg cggaactggt
     1381 tttcactggt accgacgtgt tggccgttgg agaaccgctt ctgcgcggcg gattttgctc
     1441 ggtcgtttct cccaacagca gttccaacgg cgcattggtt ggatggcgac cgcccatcga
     1501 cccgccttct aatctgcgga tcagattcgc aatctccacc gtccggtcga gccgcgctaa
     1561 cttgactaat tccagttcta agaaaatccg gggctgggag agtttgtcaa actcggtttt
     1621 gagcgcacct aaagaggaaa gcatccgcaa a
//
LOCUS       DBNGZD010000072        12239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1259705, whole genome shotgun sequence.
ACCESSION   DBNGZD010000072 DBNGZD010000000
VERSION     DBNGZD010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1259705"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1718)
                     /locus_tag="AC16GM_01925"
     CDS             complement(213..1718)
                     /locus_tag="AC16GM_01925"
                     /inference="COORDINATES: protein motif:HMM:NF021010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HIR2922462.1"
                     /translation="MRDRKLVYVTLASGLFLCTFGCSKKGTQPPPVVVPKKTAKLEAK
                     KIAGTADPAGVSNPTSAFWNSDYIEFTATPDSGSTGKTYDTSPASGCQYNLANQAPGF
                     APLVRMKAAYNDSFLYLWAQWSDTTFDRNFRRLYFDGRDSLFTGGIPAFPVDITQRDS
                     LSYSPVPDSTEWTLQLNDDKIAVMWDITDAAGNGGANSPGGRFAARGCAITCHGAGKM
                     YPDSGRTDLWVWNTSRGNPFGYVTDQYTDSIFLPAGIKDDDQEKFENENWLVTARCPR
                     PRYIFDATKAGLVPYDERYYLSATDAVAAPQLSDIEIGNTYYYRRNSFDGRSCGAFDC
                     HRSDGRGDPLRTIPDLSVIGRTLSDSLIREGLGLGSSIQIHRNYTNGIAPGVPDSTLA
                     KRLTARIRAYTGAPGYYFTPPAQPAGLPAVLNVNKNAVYDAANRRYTVIIKRPLRTYG
                     ATTLQDVQFVTTREFPFSVALMDSDGKNHAGSPLQVLKFLPMVTAELFASH"
     gene            2192..2986
                     /locus_tag="AC16GM_01930"
     CDS             2192..2986
                     /locus_tag="AC16GM_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HIR2922463.1"
                     /translation="MAPATTSNGRAGMFTEKDWVAFRKGKKPELREKLLTTYLPLVKN
                     VAGRMAMGFPRSVELSDLVSTGVIGLIEAMSHFDPKRGVKFETYAVPRIRGAILDELR
                     ALDWVPRSTRAKSREIERAVSKLENQLGRTPSERELAKSLRMSPDDLFLAIGDVSGTT
                     LLSLDELLYREEDNRQVPRVETLEDTNGTTVLGEIERQELKAFLVHAVTKLTEQEKLV
                     IALYYYEELTLKEIGEVMHISESRVSQIHTKAVGKLRGLIRDKFAL"
     gene            3024..3533
                     /locus_tag="AC16GM_01935"
     CDS             3024..3533
                     /locus_tag="AC16GM_01935"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR2922464.1"
                     /translation="MKKETEKKSSHKDPRSQEKTQKIPSDTPQRRKHVRLRIESPMTF
                     CPVPETGTIPGAAVGETKINGVILNLSRGGVLFATRTPPREHGYIVMQFRLNDGDTLT
                     DVVGKVKRVEREGDEEYFCGVEFITAGELEEMNKHTGSNNFSSAMATFDERLKDFLQQ
                     KLQSRSPVS"
     gene            complement(3546..4463)
                     /locus_tag="AC16GM_01940"
     CDS             complement(3546..4463)
                     /locus_tag="AC16GM_01940"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR2922465.1"
                     /translation="MRLEELSYPQLDCLARKTTIFFLPISPLEEHGPHLPIGVDAFNA
                     AYFAQLAAEGFLAQKRDWTCVLCPLLPVGTNVFRHIGSLRVRTRVVRDLVFDHGASLA
                     RYGFRWLVVTSAHGGPRHLVALEEACEKVSRKFGAKMICLTAGMAVDLLTGKFLAEIE
                     QKRGLKFTDQERNWLLHDYHAGWWETSMMLLLRPELVDAAYTQLPPILVKLTRARSRY
                     IPTLGKGLGYFGVPGQASAQFAQLTSQIFREKGVALLLRWIGGENVAAEVHSIFYRLL
                     IFRTAFRRWVAAFLILAAGIGLALYFAAR"
     gene            complement(4550..6124)
                     /locus_tag="AC16GM_01945"
     CDS             complement(4550..6124)
                     /locus_tag="AC16GM_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2922466.1"
                     /translation="MKVAWEVALIGLFPLSVFAQTLEYVFSPPVKVTDLPPFQTFPFI
                     YSAGQHNIAARGDTVYIVWEDIRAGDGGPRIYFAKSVDGARSFLSNVRVDDNFVPFTG
                     CHHPSMAVDQNGAIYVVWDDSRRFDLDIRFAKSTNGGQTFTPSVRVDDVNDRTVKQEF
                     ASIAVANDGLIYVVWNDYRDTLTSVYSARSTNAGLSFDNNVKVNDARAHAGFASVGAS
                     DGGRVFVAWSDLRNRQTTDLYFARSLDSGNTFQANLPVNDNLSDTTSDQAPPSLVANG
                     DKIYVAWPDKRNDDWSIYFAVSSDSGNSFFPNVKIDHAPRNTLQEFPSLAVQGNLIFA
                     LWRDQRRVPAGGEADVYFGFSANGGLSFQGDIPVDGDTLSRINSGSPSIAVNEQGTVF
                     VVFGDDRYDTLQHEAGDLFCVRGDPITTGIAINNDLPIPGFHLFQNFPNPFNGATLIT
                     FNISQSLRGSYTLEIFDLLGRKVRTLFSGHLDEGRYQAVWDGSGDNARQVASGIYFYR
                     LKGELAQAIKKMVLIK"
     gene            6498..6821
                     /gene="csrA"
                     /locus_tag="AC16GM_01950"
     CDS             6498..6821
                     /gene="csrA"
                     /locus_tag="AC16GM_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR2922467.1"
                     /translation="MLILTRKLGESITIGDDIKVTVLGIFGRQVRLGVDAPTSVIVHR
                     EEIYKRIQEENRQAAQGKPEDLAKAKEIIDETLPPERKNNTGQTGIHYKSSEPPNPKN
                     HRRPL"
     gene            7008..7556
                     /gene="fliW"
                     /locus_tag="AC16GM_01955"
     CDS             7008..7556
                     /gene="fliW"
                     /locus_tag="AC16GM_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HIR2922468.1"
                     /translation="MKNVSFVPPAAVQGEESTPAGRNAVVVTHLGEIPFTPRQIIIVP
                     KAILGFEALKRYLLVEHSGWAPFLWLVAVDQPAVTFAVVNPLLFFPGYHIEVDRRELA
                     ELEIANPAAVHTYGIVTIPHDLRLLSVNLQGPILVNTEKNLGKQVVLSKSPYCTRHFI
                     FEELRRQFQVPSRTNLKRLSAV"
     gene            7578..8480
                     /locus_tag="AC16GM_01960"
     CDS             7578..8480
                     /locus_tag="AC16GM_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922469.1"
                     /translation="MPKQDDHPTRDGDLVRKGLTLFRQGKLSQAEALLRQALAANGRD
                     PELHNNLGVILYSQKRYPEALEFFRTSIRNGPQYKDAYYNLAQLYLTTDRRQEAQATL
                     QQYLSLNGGDRDVELLLGSLFISGGEWQKASELLEKVVTNSPDNLLALNNLGLAYFNL
                     QKTGLAKAVWQKVVARDPKNVDALFALALVCQKEKAHTEALGYFEACLRLDPAKAEVY
                     NHYGVSLCHTARIAEAEEVFRQAVTFKPDYLNAWVNLGALYQVTGRLGQAKEAYEKAK
                     ALAPNEPVIEEQLAELQKRLQSED"
     gene            8641..9339
                     /locus_tag="AC16GM_01965"
     CDS             8641..9339
                     /locus_tag="AC16GM_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2922470.1"
                     /translation="MKQLALNCMVCGQRPKGIFCTLAGSHLTRLDREKTVHEYQRGQV
                     IFYEGNPAGAIHCIYSGRVKLYKTGPKGDYQIIRLLGPGEIMGYRALLANEPCAATAE
                     AVEPTTICTISKETLFDLLRQSSELAFQLLTKLAKELRVSEEQELVIAQETVRQRTAR
                     FLLFCCGGEKPKVGEPLKCPLSRAEMAQMVGTTPETFSRTLRYFALRGILRVTRWEIF
                     IENPAALELLSRSL"
     gene            9594..11561
                     /locus_tag="AC16GM_01970"
     CDS             9594..11561
                     /locus_tag="AC16GM_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR2922471.1"
                     /translation="MLRDRKSHLAGALAAAVLSVLLVGTARPAAAADPLDPATLTKYV
                     DPLPIPGVVQSVGMLGGYPYYEVTMTQFTQQLHSELPPTTVWGYHGTYPGPTFEARRG
                     KPIYVRWINNLPMEHLFTVDTGIHGAEYPENPYVRTVVHLHGGNVPHEADGYPTEWFT
                     PGQNDLNLYPNRQRATTLWYHDHALGITRLNVAAGLAGFYLIREPWERGLNLPSGPYE
                     IPIVIQDRTFDVNGQLMYPYPWEPEFFGNTAVVNGKVWPRLVVEPRKYRFRFLNGSNA
                     RFYNLKLFESDTEGNMTSTPGPAFYQIGTDGGYLSAPVMLNDPGNPFSPRLLFGSGER
                     ADLVIDFAGYEGKYFVLHNNAKAPFKGLDAPEEDEAPLSEIMLFEVSSTPVVDNSSLP
                     EYLREVEPLNPYEAVMTRDLTLEELLDAEGNPIAALLNGMMFHDPITEKPRLGTTEIW
                     RIINLTEDVHPIHLHLVQFQILDRQPFDVEHYMATKELVFTGPPEPPAPSEQGWKDTH
                     QDPPGYVTRIIARFRDFPGLFVWHCHILEHEDNEMMRPYEVIRGVKPLVPITQTSSAP
                     NLEQNYPNPFNPTTDIRYSLATDAQVNLVIYNILGQKVRELVNAQQPAGWYTAHWDGK
                     DELGRPTASGIYFARLVVGDFVDTKRMLLVK"
     gene            11761..>12239
                     /locus_tag="AC16GM_01975"
     CDS             11761..>12239
                     /locus_tag="AC16GM_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922472.1"
                     /translation="MTRKSLSTMGLLVLLLVGLAWAYPPGVGITSKSKNCLTCHVNNG
                     PWQDEANTIIDILDRETKKSLKQPDGTFLIAAHRGETKKLLTVIGRKAGDAGDSPLRN
                     GWIFIDPTRIESNSLNKFAAGWAVDLPASCRLTGDKLEGYEGAKVTVLQFTVQPGDEA
                     "
BASE COUNT         2807 a         3464 c         3536 g         2432 t
ORIGIN      
        1 ataatttaat cagaaaagga gaattttacg ggtttcggac agaatttgtt tccttgcagt
       61 tttctcctgc aagagcacgt gcgcgaagac cctggcgtcg cccatccgcc taaggcggat
      121 tgggctgaca ggtcatcccg tccattttcg cccagaccgg tgcagggaac ccgggcggaa
      181 cagggcggct atgcgcggtt gattatttcg cgttagtggc tggcgaaaag ttcggcggtg
      241 accattggca gaaactttaa cacctgcagg ggcgagccgg cgtggttttt gccgtcggaa
      301 tccataagcg ccacgctgaa ggggaactcc ctggtagtaa caaactgcac gtcctgtaaa
      361 gtagtggcgc cgtaggtccg cagcgggcgc ttgatgatca cggtgtagcg gcggttggca
      421 gcgtcgtaga ccgcgttttt gttcacgttc aagaccgcag gcaggccggc cggctgcgcc
      481 gggggggtga agtaatagcc cggtgcgccg gtgtaggcac ggatgcgggc ggtcagtctc
      541 ttcgccagag tggagtccgg tacgcccggt gcgataccat tggtgtagtt ccggtggatt
      601 tgaatggaac tgcccagtcc caagccctcc cggattaagg aatccgacag cgtccgccca
      661 atcaccgaca ggtccggaat tgtccggagg ggatcgccgc ggccgtcgga acggtggcag
      721 tcaaaggcgc cgcacgagcg gccgtcaaaa gaattacgcc ggtagtagta agtgttgccg
      781 atttcaatgt cggagagctg gggggcggct acggcgtcgg tcgccgataa gtagtagcgc
      841 tcgtcgtacg gcaccagccc cgctttggtg gcgtcgaaga tgtagcgggg acgggggcag
      901 cgggcggtca ccagccaatt ctcattttca aacttctcct gatcgtcgtc cttgattccg
      961 gccggcagga atatgctgtc ggtgtactgg tcggtaacgt agccaaacgg attgccccgg
     1021 gaggtattcc agacccagag gtcggtgcgg ccgctgtcgg ggtacatttt gccggcaccg
     1081 tgacaggtga tggcacagcc gcgcgcggca aaacgcccgc ccgggctatt ggcgccgcca
     1141 ttccccgccg catcggtgat atcccacatg acggcgattt tgtcgtcgtt caactggagg
     1201 gtccattcgg tcgagtcggg caccggggag taggagagcg agtcgcgctg ggtgatgtcg
     1261 accggaaagg cgggtatgcc gccggtgaaa agcgaatcgc ggccgtcgaa gtagagccgc
     1321 cggaaattgc ggtcaaacgt ggtgtcgctc cactgcgccc agaggtagag gaacgaatcg
     1381 ttgtaggcgg ctttcatccg caccaaaggg gcaaagccgg gagcttgatt ggccaggttg
     1441 tactggcagc cgctggcggg ggaggtgtcg taggtttttc cagtggagcc gctgtcgggc
     1501 gtcgccgtaa attcgatgta atcgctgttc cagaacgcag aagtggggtt gctcacgccg
     1561 gccgggtcgg ccgttccggc tatttttttc gcttccagtt tggccgtttt tttcggcacc
     1621 accaccggcg gcggctgcgt gccctttttg ctgcagccaa aagtgcaaag gaaaagtccg
     1681 ctggcaagcg tcacgtatac taattttcga tcccgcatat caacctccaa actgccggta
     1741 cgctctttcg ctccgcaccg gccaacagct caaagataca ctctcttttt ttgttcgcca
     1801 actcttattt ggagctcctt tttcgtcagt gacctctgag gaaccaggcg tctgcggccg
     1861 ctggggtgct cggaaaggtc ctggttttat ccagattccg cccagattac ttcgcagaaa
     1921 atgtgccgtt gagacgccaa aagagcggaa gcccgcttga tacttttagg aaatttccgc
     1981 cgcctttttc tggaaaaaag ttcccctgcc tctctttggg caacttttga ccagcccttt
     2041 tgatcatcta accctttcgc aaacagtccc ttagcaaaag ggttagccaa tgcacaaacg
     2101 gcacggggct tgcaaaaccg cccgccgatg cctcacctga catttcccct gagcgacgac
     2161 gggtgccacc caatcagttt ttcgctcgtc tttggctcca gccactacca gcaacggacg
     2221 ggccgggatg ttcactgaaa aagattgggt cgccttccgc aaggggaaaa aaccggaact
     2281 ccgcgaaaag cttttgacga cctacctgcc tctggtgaag aacgtcgccg gacggatggc
     2341 gatgggcttc ccccggtcgg tggagctctc cgatctggtc tccaccggcg tcatcgggct
     2401 catcgaagcg atgtcgcact tcgatccgaa gcgcggggtg aagttcgaga cctacgcggt
     2461 gccgcgcatc cggggcgcca tcctggacga actacgggcg ctggactggg tgccgcgttc
     2521 cacccgcgcc aagtcgcggg agattgagcg ggcggtctcc aagctggaga accagctggg
     2581 ccgcaccccc tcggaacgcg aactggccaa gtcgctgcgg atgtcgccgg acgatctgtt
     2641 tttggccata ggcgacgttt cgggcaccac gctcctctct ttggatgaac tcctctaccg
     2701 ggaggaggac aaccgccaag tgccccgggt ggaaacgttg gaagatacga acggcaccac
     2761 cgtcctgggc gaaatcgaac ggcaggaact gaaggccttt ctggtgcatg cggtcaccaa
     2821 gctgaccgag caggagaaac tggtgatcgc gctctactac tacgaagaac tcaccctgaa
     2881 agaaatcggc gaggtcatgc acatctccga gtcgcgggtg tcgcaaatcc acaccaaagc
     2941 ggtggggaag ctgcgcggcc tgatccggga caagtttgcg ctctaaagcg gccggttcca
     3001 ttcacggagg atagggaggg agaatgaaaa aagaaaccga aaagaaatcg agccacaaag
     3061 acccgcgcag tcaggagaaa acccagaaaa ttccatccga cacgccgcag cggcgcaagc
     3121 acgtccggct gcgcattgaa tcgcccatga ccttctgccc ggtgcccgaa acggggacca
     3181 ttccgggagc ggcggtggga gaaacaaaaa tcaacggagt catcttgaac ctttcccggg
     3241 gcggagtctt atttgccacc cgcacccctc cgcgggagca cggctatatc gtcatgcagt
     3301 tccggttgaa cgacggcgac accctgaccg acgtggttgg gaaagtgaag cgggtcgaac
     3361 gcgaggggga cgaggaatac ttctgcgggg tggagttcat caccgccgga gaattagaag
     3421 aaatgaacaa gcacaccggg tccaacaact tttcttcggc catggccact tttgacgagc
     3481 ggttgaaaga cttcctgcag caaaaactcc agtcccgctc cccggtgtcc tgaaaaaaac
     3541 cgctcttacc gggcggcgaa gtagagggcc aggccgattc cggccgccag tatcagaaaa
     3601 gccgcgaccc agcggcggaa agcggtacgg aagatcaaaa gacggtagaa gatcgagtgc
     3661 acctcggccg ccacgttttc tcctcctatc cagcggagga ggagcgccac gcccttctcc
     3721 cgaaaaatct gactcgtcag ttgggcgaat tgggccgatg cttggccggg cactccgaag
     3781 tagcctagac ctttccccag agtggggata taccgggagc gggcccgggt cagtttcacc
     3841 agaatcgggg ggagttgggt gtaggcggcg tccaccagtt cgggccggag caaaagcatc
     3901 attgaggtct cccaccagcc ggcgtgatag tcatgcaaaa gccagttgcg ctcttggtcg
     3961 gtaaacttta ggccgcgctt ctgttcgatt tcagctaaaa actttccggt caataagtcc
     4021 accgccatac cggcggtaag gcagatcatc tttgctccga acttccggga aactttttcg
     4081 cacgcttctt ccaaggccac cagatgcctg gggccgccgt gggcgctcgt gaccacgagc
     4141 cacctgaatc cgtagcgggc cagggaagcg ccgtggtcaa acaccagatc gcgaaccacc
     4201 cgggtgcgca cgcgtagcga gccgatgtgc cggaaaacgt tcgtccccac cggcagaagc
     4261 gggcagagaa cgcacgtcca gtcccgcttc tgcgccagaa agccctccgc agccagttgg
     4321 gcaaagtagg cggcgttgaa ggcatcgacc ccgatgggga gatgcgggcc gtgctcctcc
     4381 aagggagaaa tgggcaggaa gaagatggtc gttttgcggg ctaagcagtc gagctggggg
     4441 taggagagtt cttcgaggcg caaaagcgac ggcacgaggg gaaagtaggg actatcaacg
     4501 acctgtcaag gaactgagga attcccaatg cggactggga tgaatgagtt tatttgatga
     4561 gaaccatctt ctttatcgct tgagccagtt caccttttag tcggtaaaag taaatcccac
     4621 tggcaacttg acgagcgtta tctcctgaac catcccaaac cgcttgatac ctcccttcat
     4681 cgagatgccc tgagaataaa gtacggactt ttctcccaag gagatcaaaa atttccaaag
     4741 tgtaactgcc ccgaagtgac tgagaaatgt tgaacgtgat aagggttgcg ccgttaaatg
     4801 gattcgggaa gttttgaaaa aggtgaaaac cggggatcgg gaggtcattg tttatagcaa
     4861 tccctgtggt aatcgggtca ccacgaacac agaagaggtc gccggcttca tgctgtaagg
     4921 tgtcatagcg atcatcgccg aagaccacaa aaacagtacc ctgttcgttg acggcaatgg
     4981 atggtgaacc actattgatt cggctaagcg tgtcgccatc taccggaata tcaccctgga
     5041 aggaaaggcc accattggca ctgaagccga aatacacatc cgcttcccct cctgcgggta
     5101 ctcttcgctg gtcacgccat agtgcaaaaa tgagattgcc ttgaacggcc agcgaaggga
     5161 attcttgtag ggtgtttcgg ggggcatggt caatctttac attgggaaaa aaggagttcc
     5221 cgctgtccga acttacagcg aaataaatgc tccagtcatc gttgcgttta tctggccagg
     5281 caacgtagat tttatccccg tttgctacaa gactgggagg agcctgatca ctagtggtat
     5341 cagagaggtt gtcattgacc ggaagattcg cctgaaaagt atttccacta tccaaactcc
     5401 tggcaaagta taaatctgtt gtttgccggt tacgcaaatc gctccacgcg acaaataccc
     5461 ttcccccgtc gcttgcacct acagaggcaa aacctgcgtg ggctcgtgcg tcattgacct
     5521 tgacgttgtt gtcaaaactc aagcccgcgt ttgtgctgcg ggcggaataa accgaagtca
     5581 gcgtgtcgcg gtagtcattc cacacgacat aaatcaagcc gtcattcgcc actgcaattg
     5641 aagcaaattc ttgcttaacc gttcggtcat tcacgtcgtc aacgcgtacg gaaggagtga
     5701 aggtctgccc gccattggtg cttttcgcaa agcggatgtc cagatcaaaa cggcgggaat
     5761 cgtcccagac aacgtaaata gccccattct ggtcaaccgc catacttggg tggtggcagc
     5821 cggtgaaggg tacgaagttg tcgtccaccc gcacgttgga caagaaactc cgggctccgt
     5881 ctacactttt ggcaaagtaa atccgcgggc caccgtcacc ggcccggata tcctcccaga
     5941 cgatgtagac cgtgtcacct ctggcggcaa tgttgtgctg accggcggaa tagatgaacg
     6001 gaaatgtctg aaagggaggc agatcggtaa ccttgacggg cggggagaaa acgtattcaa
     6061 gtgtttgggc aaaaacacta agtggaaaaa gaccaataag ggctacttcc catgcaactt
     6121 tcattccagc actcccaatt acaatagtta aacgatattg aaactaaaaa attataaaaa
     6181 tctcaacaaa agcggagctt aaaactccgg aaagaatata gtttaagcac gcccttaggg
     6241 ccttagagcg gaaggcgtgg cgcaggcggc ggcagagcgt ccctctcagg agggggaaag
     6301 ggctaaaacc cgcaccagac gggcattccc tgcgtattag gagatggaaa gtcagttcct
     6361 ccagcactcc gccagattta ccaaccgaca gccgtgccgg tgctcccggg gaaaagacgg
     6421 gcggcgattc aagccacgct gcctttggcc gataactgaa gcacaggcag tcggtacgac
     6481 cgtacgggca gaaggccatg ctgattttaa cacggaagtt gggcgagagc atcaccatcg
     6541 gtgacgatat caaggtcacc gttctgggca tcttcggccg ccaggtgcgg ctgggcgtgg
     6601 atgcgccgac ttcggtgatc gtccaccggg aagaaatcta caagagaata caggaggaga
     6661 accggcaggc agcccagggc aagccggaag acctcgccaa ggccaaagag attatcgacg
     6721 aaaccctgcc tccagaaaga aagaataaca ccggccagac aggcatacac tacaaatcat
     6781 ccgagccgcc caacccgaag aaccaccgga gacccttgta ggtatgtggc actggaacaa
     6841 acccaaaaag acaaaagaac aaacttccgc agcgccggac cgccgaccgg ccgggccatc
     6901 tgtgaccaac cgcagtcgct tcgcaccggg tcagcgcgca ccgcagactc cccgccggga
     6961 tttacaaccc gatctcgacc gggggattga cgccgggttc gtttactatg aagaacgtta
     7021 gtttcgtacc gccggccgcc gttcaaggcg aagaatcaac cccagcggga cggaacgccg
     7081 tcgtcgtgac ccacctgggc gagattcctt tcactccacg gcagatcatc atcgttccga
     7141 aagcgattct gggatttgag gcgctcaagc ggtacctcct ggtggagcac tccggctggg
     7201 cgcctttcct gtggcttgtg gccgtcgatc agccggcggt cacttttgcc gtggtcaacc
     7261 cgcttttgtt cttccccggc taccacatcg aagtggaccg ccgcgaattg gccgaactgg
     7321 aaatcgccaa tcctgcggcc gtgcacacct acggcatcgt caccatcccg cacgacctcc
     7381 ggctgttgtc ggtcaatctg caggggccga ttctggtcaa caccgagaag aacttaggca
     7441 agcaggtggt gctttccaag agcccctact gcacgcgcca cttcatcttc gaggaactcc
     7501 ggcggcagtt ccaggtgcca agccggacca acctgaagcg gctgtccgcc gtttaattcg
     7561 aaccgatttt cattcctgtg ccgaagcagg acgatcatcc gacgcgcgac ggggatctgg
     7621 tccgcaaagg attgacgctt tttcgccagg gaaaactctc ccaggcggag gcgcttttgc
     7681 ggcaggcttt ggctgccaat ggccgcgacc cggagttgca caacaacctc ggcgtcattc
     7741 tctactccca gaagcggtat ccggaagcct tagagttttt ccggaccagc atccggaacg
     7801 ggccgcagta taaagacgcg tactataact tagcgcagct gtacctcacc accgaccgcc
     7861 gccaggaggc acaggcgacg ctccagcagt atctttccct gaacggcgga gaccgggacg
     7921 tcgaactact cctggggagt ctattcattt ccggtggaga gtggcaaaag gcatctgaac
     7981 ttttggaaaa ggtggtgacc aactccccgg acaatcttct ggcgctgaac aacctcggtc
     8041 tggcctattt caatttgcag aagaccggct tggccaaagc ggtctggcaa aaggtggtgg
     8101 cgcgtgaccc caagaacgtc gatgccctgt ttgcgctggc gttagtctgc cagaaagaga
     8161 aagcgcatac ggaagccctc ggctactttg aagcctgcct gcggctggat cccgcgaaag
     8221 ccgaagtgta caaccactac ggggtcagtc tctgccatac ggcccgcatc gccgaagccg
     8281 aggaggtgtt ccgtcaggcg gttaccttta agcccgacta tctcaatgcc tgggtgaatc
     8341 tgggggcact ctaccaggtc acgggacgcc tgggacaggc caaggaggcc tacgaaaaag
     8401 ctaaggccct ggcgccgaac gagccggtga ttgaagaaca gttagccgaa ctgcaaaaac
     8461 ggctgcagtc agaagattag ctcacacctc tgtcggccaa tttcgtcgta cctctctcaa
     8521 tacaacgaat aattcgtaac cggtttcaat tgacaaccac ctcggtctcc tattttttgt
     8581 ccgaatgaat accttcttca gatgtcgatc tttcgactgg ccttctgagc cggtagaaaa
     8641 atgaaacagt tagctctcaa ttgcatggtc tgcggccagc ggccgaaagg tatcttctgc
     8701 actctggcag gttctcactt gacccggctt gatcgcgaga aaaccgtcca cgaataccaa
     8761 cgggggcagg ttattttcta tgagggtaat ccggctggag cgattcactg tatctactcg
     8821 gggcgggtga aactctacaa aacgggaccg aaaggcgatt accagatcat tcggctcctg
     8881 ggaccggggg agattatggg ttaccgggcc ttgttggcca atgagccctg tgccgccacc
     8941 gcggaagccg ttgagccaac caccatctgt accatctcga aggagactct ttttgatctc
     9001 ctgaggcaat catccgaact ggccttccag cttttgacca aactggccaa agagctgcgc
     9061 gtctccgaag agcaggagtt ggtcatcgcc caggaaacgg tgcggcagcg caccgcccgc
     9121 tttctgctct tttgctgtgg gggagagaag ccgaaagtag gggaaccgct caagtgccca
     9181 ctttcccggg ctgaaatggc ccagatggtg gggacaacgc cggagacatt ctcccgcacc
     9241 ctgcgctact ttgccctgcg gggcatcctg cgcgtgaccc gctgggagat cttcatcgaa
     9301 aatcccgccg ccttagagct tttatcccgc tcgctctaat cccgacttga cgccgatcaa
     9361 gccggcgctt gacacccatc attggaatta aaagagtatt atatattttc atacttgatt
     9421 cgtctccatt ctgtacgacc gcaccgaacc agcacagccg accataagag gatgcgatgc
     9481 ggccagtgga acaatagagg ggaggaagaa ggtatgacag cgagcggagg gtaaaaagca
     9541 gaatgtctgt gagcagagga agtaaacaag gtgacgggaa ggagaagcaa gcaatgctga
     9601 gagacagaaa atcgcatctg gccggggcgc tggctgcggc ggtgctgtca gttcttctgg
     9661 tagggactgc ccggccagca gcggcggctg acccgctgga tccggcgacc ctcacgaagt
     9721 acgtagatcc gttacccatc cccggcgtgg ttcagtcggt ggggatgctc ggcggatacc
     9781 cctactacga agtgacgatg acgcagttca cccagcaact gcacagcgag ctccccccga
     9841 cgacggtgtg ggggtatcac ggtacctacc ccggaccgac cttcgaggcg cggcggggga
     9901 agccgatcta cgtccgctgg atcaacaact tgcctatgga gcacctcttt accgttgata
     9961 ctggcattca cggtgctgaa tatcccgaaa atccctatgt ccgcaccgtg gtacacctgc
    10021 acggcgggaa cgtcccgcac gaggcggacg gttaccccac tgagtggttc acgcccgggc
    10081 agaatgacct gaacctgtat cccaatcgtc agcgggccac caccctctgg tatcacgacc
    10141 acgctctggg gattacgcgc ctaaacgttg ccgcagggct ggcgggcttc tacctgatcc
    10201 gggagccctg ggagcgcgga ctcaatctgc cgagcggacc gtacgagatt ccgatcgtca
    10261 tccaggaccg gaccttcgac gtaaacggcc aactgatgta cccctacccg tgggagccgg
    10321 aatttttcgg gaacacggcg gtggtgaacg gcaaggtctg gccccgtctg gttgtggagc
    10381 cgcggaagta ccgcttccgc ttcctgaacg gctccaacgc acggttctac aacctgaaac
    10441 tgttcgagag cgacaccgag gggaacatga cctccacccc cgggccggcc ttctaccaga
    10501 ttgggacaga cggcgggtat ctctccgccc cggtgatgct caatgatccg ggcaacccat
    10561 tctccccccg gcttctgttc ggctccggtg agcgggcgga tctggtgatt gactttgccg
    10621 gctatgaggg gaagtacttc gtcctccaca acaacgccaa ggcacccttc aaagggctgg
    10681 atgctccaga ggaagacgag gcgccactat ccgagatcat gctctttgag gtgagcagca
    10741 cgccggtcgt ggacaacagt tcgctccccg agtatctgcg cgaagttgaa cccctcaacc
    10801 cgtacgaagc ggtgatgaca cgagatttga ctctcgagga actcctggat gccgaaggca
    10861 acccgattgc agcgctcctg aacggcatga tgttccatga ccccatcacc gagaagccgc
    10921 gtctgggtac taccgaaatc tggcgtatca tcaacctgac cgaggacgtg caccccatcc
    10981 acctccactt ggtacagttt cagattctcg accggcagcc gttcgatgtc gagcactaca
    11041 tggcaaccaa agaactggtc tttaccgggc ctccggaacc gccggctccc agtgaacagg
    11101 ggtggaagga cacccaccag gatccgccgg ggtatgtcac ccgcatcatt gcgcgcttcc
    11161 gcgacttccc cggacttttc gtctggcact gtcacatctt agaacacgaa gacaacgaga
    11221 tgatgcgtcc gtacgaggtc atccggggag tgaaaccatt ggtaccgata acgcagacat
    11281 cttccgcccc gaacctggag cagaactacc ccaacccgtt caatccgaca accgacattc
    11341 gctactcgct ggcgacagat gcgcaggtga acctggtcat ctacaacatc ctgggtcaga
    11401 aggtccggga gttggtgaat gcccagcagc ccgcgggctg gtacaccgcg cactgggatg
    11461 ggaaagatga actgggccgg ccaacggcca gcggcattta cttcgcccgg ctggtagtgg
    11521 gtgacttcgt ggacaccaag cggatgctcc tggtaaagta aagggcatcc gcagcagaag
    11581 agggcgctcc aaccggagtg ccctcttttt ttgacgttgg tgcgaatctt gcaaaggaaa
    11641 cggccacggg gtaaagattt cacccggcgc tgttggttct ggtgtacaac ttgcttgaca
    11701 attccggtga tctctcgttt tttgacagaa acggagtttc ttatcagaaa aggaggagcc
    11761 gtgaccagaa aatctctcag caccatgggt ttgctcgtgc tacttctcgt cgggttggcg
    11821 tgggcctacc cgcccggggt ggggatcacc agcaagtcca agaactgctt aacctgccac
    11881 gtcaacaatg gcccctggca agatgaggcg aataccatca ttgacatcct ggaccgagaa
    11941 accaagaaat ccttaaaaca gccggatggc acatttctca tcgccgctca ccgcggcgag
    12001 accaagaaac tcttgaccgt gatcgggagg aaagctggcg atgcgggcga tagtccgtta
    12061 cggaatggct ggattttcat cgacccgacc agaattgaaa gtaattccct gaacaaattc
    12121 gctgccggct gggcggtcga tttgccagct tcctgtcgtt tgacagggga caaattagaa
    12181 ggatatgagg gggcaaaagt cacggtgctg caatttacgg tccaacctgg cgatgaggc
//
LOCUS       DBNGZD010000073         6042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_288216, whole genome shotgun sequence.
ACCESSION   DBNGZD010000073 DBNGZD010000000
VERSION     DBNGZD010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_288216"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="AC16GM_01980"
     CDS             complement(<1..1188)
                     /locus_tag="AC16GM_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2922473.1"
                     /translation="MLKYRIDGVADNFNLGYSVAGAGDVNGDGYSDFIIGAPGRGIGS
                     GLVLVYSGRTGTFLHVLYGNTGERFGWSVAGSGYVNQEGTTDFVVGAPFAGTGGLPSA
                     GSVYLYSGASGNLIRRINGTAAGDHVGWSVAGAGDVNSDGWADIVVGAPGIPGYSLGR
                     AFVYSGKTGVALFQKTGDDRVCPDFSWVCYTDRLGFAVAHSDDVNDDGRPDFIIGVPF
                     ADTNGVESDFVDVGSAIVFSGATGDTLFRINGNEGDRLGYSVAGTGDVNSDGRADFIV
                     SAPGADPAGLSSAGSVFVYSGARGNLLYRVNGTARGENFGLSVAGTGDVNGDGVPDFV
                     IGAPLADPNGLIDAGSAYLYSGRDGSLLFQIDGTAAGDQLGQSVAGAGDLDGDGRAEF
                     IVGA"
     gene            complement(1292..2776)
                     /locus_tag="AC16GM_01985"
     CDS             complement(1292..2776)
                     /locus_tag="AC16GM_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2922474.1"
                     /translation="MAILLFAWGGILASPVAYSQGFVLKYRIDGVADNFNLGYSVAGA
                     GDVNGDGYSDFIIGAPGRGVSSGLALVYSGRTGQLLHVLYGSDGERFGFSVASTGYVN
                     QDRTTDFIIGAPYANPPGLLHAGSVYLYSGATGELIRRINGTAAGDHVGWSVGGFGDV
                     NEDGRADFLVGAPGFFGEIPGFGSAIVYSGKIGTVLYQQRGVSGENLGYSVAEVGDLN
                     GDGRADFIVGAPLANPPGYPATGGSAFVYSGATGNLLFQIDAATDDDQLGFSVAGAGD
                     VDGDGRADFIIGAPAHDPGEITNAGSAFVYSGATGTLLYQKDGSAAGDNLGYAVAIAG
                     DVNGDGRADFLVGSPFAAPSGLAYAGSAFLYSGRDGSFLFQIDGTAAGDQLGQSVAGA
                     GDLDGDGRAEFIVGAPFADPHGLAAAGTAYVFSLPCLPRGDVDLSGVTTIEDAVALLR
                     QVVFGEPLPNPTVGDVNCDGVWDLVDLVLLVQFIVFGSPSPCCL"
     gene            3381..4640
                     /locus_tag="AC16GM_01990"
     CDS             3381..4640
                     /locus_tag="AC16GM_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922475.1"
                     /translation="MNLLGQIQAALAEAGEQVTSDLVALINRRIRPPVPVAIDNVHIR
                     TMFVISDQVNSYGGRLPLEEHPNVARLLVDAPVLIGHAKDRLPVGRNFKAELVEKNGQ
                     RWIKVWFYWLKGTAEAESLAQNIDHGIYKECSIGFFFDFPECSVCGKDIRACAHVPLS
                     TFQSADGSEQKVSFNYRGVRKVLETSLVYRGAVPETALSNELFAKAGGNSVGVRFIEP
                     APVGARRAEPPPKIAPAQKNPNGTFELRRQEKSKKPKTLLILSLETETKVFALENFDL
                     NRLPTGKRFRCIPIDPKRINGEKNSFALLDTGACKVIGKDELVLTGKVLCGQISLALA
                     PSQGTFLSQSRVCDTSSEDKGSTTSPDSVQGPVRGVRLEQNAKTTPALVDRSDRSVRT
                     VPEPVKDRVGAAPSPREEGFREQENAR"
     gene            4630..5088
                     /locus_tag="AC16GM_01995"
     CDS             4630..5088
                     /locus_tag="AC16GM_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922476.1"
                     /translation="MLDNRQPIVKENWLNARGVWLRFLVTFAVTAGGILAAYYSTIGR
                     IDLALARKADQEQVASLDKKLSTLEVLLTTNAVTREEFHQFRDEVVQRLGRIEARLEP
                     QSRGQTIPGDESSLPFEGRGLRHLSSPYQGEGREGSEFRASSTREYRDDD"
     gene            5075..5557
                     /locus_tag="AC16GM_02000"
     CDS             5075..5557
                     /locus_tag="AC16GM_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922477.1"
                     /translation="MTTTELIHQLKVHLNLASPDRMTEVDFRQLQRDLDSAVEKLSEM
                     ERTQTELALRARWSDVLAEALVQEMKGKLLALRLTGEASADWPLWQKLIAAAGSPEEF
                     LRLRELIHRKFDSTFPNRLHAQAVGSENGNHKKEIVMEEFKTGSTPRRRHFSAAPLFS
                     "
     gene            5672..>6042
                     /locus_tag="AC16GM_02005"
     CDS             5672..>6042
                     /locus_tag="AC16GM_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922478.1"
                     /translation="MGIRDQNLEGIGAVFATFTIRQTASVDDLKDADVGKAVTITASN
                     QAAPTSDGSILLGKLVDLTLTDADNGKRVATVQIGGVVTLPVVATVPVVGNRVVGGAG
                     GTVKQAPVLAAFDPAGGNIARG"
BASE COUNT         1444 a         1931 c         1536 g         1131 t
ORIGIN      
        1 cgcgccgacg atgaattcgg cccgcccgtc tccatccaga tcccccgccc cggccaccga
       61 ctggcctaat tgatcccccg ctgcggtgcc gtctatctga aagaggagtg agccgtcccg
      121 gccagaatag agatatgccg aaccggcatc gataagacca ttggggtcgg ccagtggagc
      181 cccgatgaca aaatcgggca caccatcgcc attcacatcc ccggtaccag ctaccgacag
      241 gccaaaattt tcaccacggg cggtaccatt aacccggtac aagagattac ccctcgctcc
      301 ggaatagaca aaaaccgatc cggcgctcga aagtcccgcc gggtctgcac ccggagcgct
      361 gacgatgaaa tctgccctgc cgtcgctatt cacatcgccc gtcccggcta cggaatagcc
      421 caatcggtcc ccttcattgc cattgatccg aaataaggtg tcacctgtcg cacccgaaaa
      481 gacaattgcc gagccaacat ccacaaaatc gctctcaaca ccgtttgtgt cagcaaaggg
      541 aacgccaatg ataaaatccg gtctaccatc atcattcaca tcgtcggagt gagctacggc
      601 aaaaccgagc ctgtcagtgt aacaaaccca agagaagtcg gggcatacac gatcgtcgcc
      661 ggttttctgg aatagcgcaa ctccagtttt gccggaatag acaaaggctc tgccgagaga
      721 gtatccgggg attcccggag caccaaccac aatgtcggcc cagccgtcac tgttcacgtc
      781 tcccgctcct gccaccgacc atcccacatg atcgcccgcg gccgtgccgt ttattcgtcg
      841 aataagattg ccagaggctc ccgaatagag atagaccgaa ccggcactgg gaagccctcc
      901 cgtaccggca aacggagcac cgacgacaaa atcggttgtt ccctcctggt tcacataccc
      961 cgatcccgcc acagaccacc caaaccgctc tccggtattt ccatagagca cgtgcaaaaa
     1021 cgtccctgtc cgtccggaat aaaccagcac caaccccgac ccaatccccc gccccggcgc
     1081 accaatgatg aaatcgctat agccatcacc atttacgtcg ccggctccgg caaccgaata
     1141 ccccagattg aaattatccg ccaccccatc aatccggtac ttcagcacga atccctgacc
     1201 gtatactgat gaaagcggaa aacacagaac caaaagtgcc gcccatcgac acgaggtctt
     1261 tttgaacacc ggcctcacta atttctgccc ttcatagaca acaaggactt ggactcccaa
     1321 aaacgatgaa ctgcaccaga agtaccaagt ctaccaaatc ccacaccccg tcgcagttga
     1381 catcgccaac agtaggattg ggcaacggct ccccgaacac cacctgccgt agtaatgcaa
     1441 ctgcatcctc gatcgtggtc accccgctca aatccacgtc tccgcgcggc agacagggca
     1501 aggaaaaaac ataggccgtg cccgccgcgg ccagcccgtg cgggtcggca aacggcgcgc
     1561 cgacgatgaa ttcggcccgc ccgtctccat ccagatcccc cgccccggcc accgactggc
     1621 ctaattgatc ccccgctgcg gtgccgtcta tctgaaagag gaacgagccg tcccggccgg
     1681 aatagagaaa ggccgagcca gcgtatgcga gcccgctcgg agcggcaaac ggcgacccca
     1741 ccagaaaatc ggctctccca tcaccattca catctccggc gatggccact gcatagccca
     1801 aattatcacc tgctgcactt ccgtctttct gatacaaaag cgttccagtg gctccggaat
     1861 agacaaaggc cgaaccggcg ttcgtaattt cacctgggtc atgagcggga gcgccaatga
     1921 taaaatcagc tcttccatcg ccgtccacat ccccggctcc ggctactgaa aaacctaact
     1981 ggtcgtcgtc ggtagcagca tctatctgga acaacaaatt cccggtagct cccgaataaa
     2041 caaatgcgga acctcctgtg gcggggtatc ccggcggatt agccaaaggt gcaccaacaa
     2101 tgaagtcggc tcttccatcg ccattcagat ctcctacctc tgccaccgaa taccccagat
     2161 tctccccgct gacgcctctt tgttgataca aaacggtacc aatcttaccg gaataaacga
     2221 tggcagaacc aaatcccggg atttccccga agaaacctgg agcacccacg agaaaatcgg
     2281 ctctcccatc ctcattcacg tctccgaatc cccccaccga ccatcccaca tgatcgcccg
     2341 cggccgtgcc gtttattcga cgaataagtt cgccggtggc tcccgaatag agatagaccg
     2401 aaccggcgtg tagtagtccg ggcggattgg cgtaaggtgc gccgatgata aaatcggttg
     2461 ttctgtcttg gttcacataa cccgtactcg ccaccgaaaa accgaaccgc tctccatcgc
     2521 tcccgtagag cacatgtagc aattgtcccg ttctgccgga atagactaag gccaggccgg
     2581 atgatacgcc ccgccccggc gcaccaatga tgaaatcgct atagccatca ccatttacgt
     2641 cgccggctcc ggcaaccgaa taccccagat tgaaattatc cgccacccca tcaatgcggt
     2701 acttcagcac aaagccctgg ctgtacgcaa cgggagaagc caggattccg ccccaggcga
     2761 aaagcaaaat cgccataaga actaatcgtg tcggaaatgg gattactttg cgttgccttt
     2821 gactgcccac aagaaaaccg cttgccatac gcataacggc tccttttcca gcagtggcga
     2881 gattatcgtc cgcgccggtg gcttaaactc caccgcctcc cctcgtggat caagttacct
     2941 cttttggcag ccggttgtca agaagttttt tccaccgccc tgttgactat aaatcgagcc
     3001 ccactgactc atcacccccg agcccctaat cacttatcac gactcactgt ttttttcggg
     3061 gtcattccgg ggggtaaaac gaacttcccc ctttttcttt cccataggcg gcgctatcag
     3121 cccgccctac ctgttttaag atgttttcgt gcccaccgcc tccaatgtca cccatcccat
     3181 ctcattgcgc tgcaccttaa tccgcttaat ccgcttcaaa atccgttgtc agaatttggg
     3241 gtcgccacgg gggtaaaacg gacaaagtgc gaaatagtag aggcactggt cgggaagagc
     3301 aggcgccagt agccagttac caggggccag gtaaatccag aagccctgtg aatacaaagg
     3361 aaacgggtta cgagaaaacg atgaacctcc tcggtcaaat acaagcagca ttagcggaag
     3421 caggcgaaca ggtaacgagc gacctggtgg cgctcattaa ccggcgcatc cggccacccg
     3481 tcccggtggc gattgacaac gtccacatcc gcacgatgtt cgtcatctcc gatcaggtca
     3541 actcctacgg cggacgtctg cctttggaag aacatccgaa cgtggcgcgg cttttggtcg
     3601 atgcgccggt tctgatcggc cacgccaagg accggctgcc ggtggggcgg aatttcaaag
     3661 ccgagttggt ggagaaaaac ggccagaggt ggatcaaagt ctggttctac tggctcaaag
     3721 gaacagcgga agccgaatcg ctggctcaga acatcgatca cggcatctac aaggaatgct
     3781 ccatcggctt tttctttgac ttcccggaat gctcggtctg cggcaaagac atccgcgcct
     3841 gtgcgcacgt cccgttatca acgtttcaga gcgccgacgg ctctgaacag aaagtctcct
     3901 tcaactaccg cggcgtgcgg aaggtgctgg agacctccct ggtctaccgc ggcgcggtgc
     3961 cggagaccgc cctctcgaac gaactcttcg ccaaagccgg tgggaattcc gtaggggttc
     4021 gattcatcga acccgcgccc gttggggctc ggcgtgccga gccccctccc aagatcgccc
     4081 ccgcgcagaa aaacccgaat gggacttttg aactgcggcg acaagagaaa tcgaagaagc
     4141 ccaagacgct tttaatactc tcgctggaga cggagacgaa agtctttgct ctggaaaatt
     4201 ttgacctcaa tcgtcttccc accggcaagc gcttccgctg tatacccatt gatccgaagc
     4261 gaatcaatgg agagaaaaat tcgtttgcgc ttctcgacac cggcgcgtgt aaagtgattg
     4321 gtaaagacga attggtgctt accggaaaag ttttatgcgg ccaaatctcc ctcgctctgg
     4381 caccctccca gggcaccttc ctctcgcaga gtcgagtctg cgacacctcc tctgaggata
     4441 aagggagcac cacctccccg gactctgttc aaggcccggt ccggggagtc cgcctggaac
     4501 agaacgcaaa aacaacacct gctctggtag accggtcgga cagatccgta cggaccgtcc
     4561 ctgagccggt caaagatcgc gtgggcgcgg cgccctcgcc gcgcgaagaa gggtttaggg
     4621 aacaggaaaa tgctcgataa tcgccaacct atcgtcaaag aaaattggct gaacgcccgt
     4681 ggcgtctggc tccggttcct cgtgaccttc gccgtcaccg ccggcggcat cctcgcggcc
     4741 tactactcga ccatcggtcg tatcgacctc gctctggccc gcaaagccga tcaggagcag
     4801 gtcgcaagtc tcgataagaa actttccacc cttgaagtgc tcttgaccac gaatgctgtg
     4861 accagagaag agttccacca gttccgtgac gaggtggttc agcgcctggg ccgaatcgag
     4921 gctcggctcg aaccgcaaag cagagggcaa accatccccg gagatgaatc ctccctcccc
     4981 tttgagggga ggggtctccg acacctttcc tccccttacc aaggggaggg tagggagggg
     5041 tcagaatttc gtgcctcctc cacgagggaa taccgtgacg acgactgaac tcattcacca
     5101 gctgaaagtg catctgaatt tggcctcacc cgaccgaatg actgaagtcg atttccggca
     5161 attacaaaga gacctcgact ccgccgtcga aaagctctct gaaatggagc gaactcaaac
     5221 cgaactcgct ctgcgcgccc ggtggagcga cgttctcgcc gaagcgctgg tgcaggaaat
     5281 gaagggcaaa ctcttggccc tgcgcttgac cggcgaagcc tccgccgatt ggcccctgtg
     5341 gcagaagttg attgccgccg ccggaagccc ggaggaattc ctgcgcctgc gggaactgat
     5401 ccaccggaag ttcgacagca cattcccgaa ccggctgcac gcgcaggccg tgggtagcga
     5461 gaacgggaat cacaagaaag aaattgtgat ggaggaattc aaaaccggct caacaccgcg
     5521 taggcggcac ttcagtgccg ctcccctctt ctcgtaggag agggctggtc ggtacgaccc
     5581 gtatgggggt gaggtctccc ccattctcca taggcggggc atcttgcccc gcagaaagga
     5641 aatttgaaaa cgcaaaccaa aggagcaagc aatgggcatc cgtgaccaga acttagaagg
     5701 catcggcgcc gtcttcgcca ccttcaccat ccgccagacc gccagcgtgg atgatttgaa
     5761 agacgccgac gtcggcaaag ccgtcaccat caccgcctcg aatcaggccg caccgaccag
     5821 cgacggctcg atccttctgg gcaaactcgt cgatttgact ttgaccgacg cggacaacgg
     5881 gaaaagagtc gccaccgtgc agattggcgg agttgtgacc ctgccggttg tggcaacagt
     5941 gccggtggtg ggcaatcgcg tcgtgggcgg cgccggtggg acagtcaagc aagcccccgt
     6001 cctggccgcc tttgatcccg ccggcggcaa catcgcccgc gg
//
LOCUS       DBNGZD010000074         1345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1330526, whole genome shotgun sequence.
ACCESSION   DBNGZD010000074 DBNGZD010000000
VERSION     DBNGZD010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1330526"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="AC16GM_02010"
     CDS             <1..918
                     /locus_tag="AC16GM_02010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR2922479.1"
                     /translation="PAVVAVIEQARAGHSFSQPRELRVPLAGREAIFMVTSAQIQDEA
                     DGLLGVCVLGTDVTELRRLQEELVTKEKLAALGEMAAGLAHQLRNALAAISGFGKLLH
                     RVAPEESQVKSVAAEILREAEATAALLDRFLSFAQPQKLAAEVVSAADLWQEVTAAKS
                     VDLKERNISCTVNLPPGLVIQGDLHLLKQVFVNLLQNAIEAVPQNGKIEITGVAGPEI
                     VELAISDNGPGVPKALRTRIFDPFFTTKESGSGLGLSVARKVLELHGGTLTVRDQAGG
                     GARFILTFPRQKATEDDAPLVLASSAPHQ"
     gene            complement(967..>1345)
                     /locus_tag="AC16GM_02015"
     CDS             complement(967..>1345)
                     /locus_tag="AC16GM_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2922480.1"
                     /translation="VDYIDPVVTFASDNTLSSSVAAESLAIGSRAPGVYYYEVRARDA
                     QGQWGNFSVPDSLNVVGHPRFAKGDVNQDGTRDLTDIVMLIQYVVFGDVIFADPSLAD
                     LDNDSVVDLTDIVALINIVVFGG"
BASE COUNT          301 a          402 c          400 g          242 t
ORIGIN      
        1 cccgcggtcg ttgccgtcat tgaacaagcg cgggccggac actcgttttc ccaaccccgg
       61 gagttgcggg ttcccttggc cggacgcgaa gcgattttta tggtaaccag tgctcagatt
      121 caggacgaag cggacggcct gttaggagtc tgcgttctgg gaaccgacgt cacggaatta
      181 cgccgtctgc aggaggagct ggtcaccaaa gagaaattag ccgcactggg agaaatggcg
      241 gccggtctgg cacaccagct gcgcaacgcc ctcgcggcaa taagcgggtt cggaaaactt
      301 ttgcatcgag tcgctcccga agagtcgcag gtaaaatcgg ttgccgcgga gattctccgg
      361 gaagccgaag cgaccgccgc gctcctcgac cggttcctct cctttgcgca gccgcaaaaa
      421 ctggccgcag aggtggtgtc ggcggccgac ctctggcagg aggtcactgc ggcgaaaagc
      481 gtcgacttga aggagagaaa tatcagctgt accgtgaacc ttccgcccgg gctggtgatc
      541 cagggcgacc ttcacctgct gaagcaggtc tttgtgaacc tgttgcagaa tgcgatcgaa
      601 gccgttccgc agaatgggaa aatagagatc accggcgtcg ccgggcccga aattgtggaa
      661 cttgccataa gtgacaatgg accgggcgtc ccgaaagccc tgcggacccg catttttgat
      721 ccgtttttca ccaccaagga aagcggtagc ggtctgggcc tgagcgtggc ccgcaaggtg
      781 ctggaattgc acgggggaac gctgaccgtg cgcgaccagg ccggcggcgg agctcgtttc
      841 atcctcactt tcccccggca aaaagcgacg gaagatgatg ctccactcgt cctggcatcc
      901 tcggcaccgc accaataaaa aatcccgccc gctgagcggg atcgcaagct cggcagcgcc
      961 accgggctac ccgccgaaca caacgatatt aatcagagcg acaatatcag ttagatcgac
     1021 cacgctgtca ttgtccaggt cggccagtga cggatccgcg aagatgacgt ccccaaagac
     1081 gacgtattga atgagcatga cgatgtcggt aaggtcgcgc gtcccatcct gattgacgtc
     1141 ccctttggca aagcggggat gaccgaccac gttcagactg tcaggcacgc tgaaatttcc
     1201 ccactgcccc tgagcatccc gtgcccgcac ctcatagtag tacactcccg gagcacgcga
     1261 cccgatggcg agcgattcgg ccgccaccga actggaaaga gtattatccg acgcaaaggt
     1321 gaccaccgga tcgatgtaat cgacg
//
LOCUS       DBNGZD010000075         3200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_80412, whole genome shotgun sequence.
ACCESSION   DBNGZD010000075 DBNGZD010000000
VERSION     DBNGZD010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_80412"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..887)
                     /locus_tag="AC16GM_02020"
     CDS             complement(120..887)
                     /locus_tag="AC16GM_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922481.1"
                     /translation="MNMFSRKRIVKIGIVSIPGKRREKNRLKYVSAHYEQAFFSVRDS
                     LSGKYPVEQINLKKLAKFERLFKRGIQILVLPEAGVITHQQLNNLEAFVKLGGIVFAD
                     YPSLAFDALDTVDNDATRDGGRSKILERLRHLFGVEEASETQVSLAVDTQNGGYPNLF
                     KGAPKFVNFKGKAILVMRSNCHTHGILKIENIESSDNNLDINRIKQRNIPCLLFKDYN
                     YNGYFIYATIPVCRNLPNSGLGAIFDELVHIQEEKIR"
     gene            complement(1010..1417)
                     /locus_tag="AC16GM_02025"
     CDS             complement(1010..1417)
                     /locus_tag="AC16GM_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2922482.1"
                     /translation="MFTTGLSEIVLVVKDVAASARFYREVVGLTLNEAAASDKNWAWF
                     WAGVPGHPQSVAVTQDKDLLFAEKSPLPEGQRWGQVHYAFNVPREKLAEAVEHVRGKG
                     VEVYGPKHFKWMKATAYYFYDPDGNLLEFWSPD"
     gene            complement(1481..2119)
                     /locus_tag="AC16GM_02030"
     CDS             complement(1481..2119)
                     /locus_tag="AC16GM_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="resistance to Congo red protein"
                     /protein_id="HIR2922483.1"
                     /translation="MRERYEEAKKDLEKNSFVKRVEFREYSEGGMHHFSLDVTVRQFQ
                     NLNEIQKKIPPYIEDSGEESDFRIEKLPSGNISFRQKFGESPAESTEAEADTSERNPW
                     LKFGEFMAKASISTAFGNRYFTVRLHAPKIVSTNGNLDDRMQTAEWKLALVDLLSENP
                     YEQELKAEMHPPGKGLLWIVAAILLVAVIGGITLVTMRRRKRGVEPMAPAGN"
     gene            complement(2344..2961)
                     /locus_tag="AC16GM_02035"
     CDS             complement(2344..2961)
                     /locus_tag="AC16GM_02035"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF017778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR2922484.1"
                     /translation="MKAVAGTTVNDLIEELTAICSNAKGDAQVPKVKDCLSRFLEGNL
                     ELPPQYLQPAKDHYARHLLYRQPNGGFTIVVMVWSPGQATPVHDHAGTWCVEGVYQGS
                     MKVERFHLRPLANDGERSRTTDQNIVQMTAGEILFPGKGQTGALIPPVEHHRMGNESR
                     NVAVTIHVYGGELSKCKVFEPLGNERYLAKDKTLTYTTERPVVVA"
     gene            complement(2999..>3200)
                     /locus_tag="AC16GM_02040"
     CDS             complement(2999..>3200)
                     /locus_tag="AC16GM_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922485.1"
                     /translation="FLAEYVNSLEHEWFKRTTNYKVTTAGQTTEDRTEVTRRTLRFNP
                     EHRGGVPLVSFGSVKFGLSVYF"
BASE COUNT          769 a          872 c          694 g          865 t
ORIGIN      
        1 cccaacagta tttcccgctc tacctgaaag agatggagtt ccggtacaac caccgcaaag
       61 agcccatgtt cgaacgattg gcacaactaa tggcaaatcc cgtgtcaaat cttttataat
      121 cacctaatct tttcttcctg aatatgcaca agttcatcaa atatggctcc caatccggaa
      181 tttggcagat tccggcatac aggaatcgtc gcgtaaataa agtaaccatt gtaattataa
      241 tccttgaata gaaggcaagg aatattcctt tgctttatcc gatttatgtc taagttattg
      301 tcagaacttt caatattttc tattttcaaa atgccgtgcg tatggcaatt tgaccgcatc
      361 actaaaatcg cttttccctt aaaattaaca aactttggtg cgcctttaaa gagattagga
      421 tacccaccat tttgcgtatc aactgctaaa gaaacttgag tttctgaagc ctcttcgacc
      481 ccaaagagat gacgaagccg ctcaagtatt ttacttctgc cgccgtctcg tgtcgcgtca
      541 ttatccacag tatcaagagc gtcaaaggca agagatggat aatcggcgaa gactataccc
      601 ccaagtttta caaaggcttc aagattattt aattgttgat gagtaataac cccagcttca
      661 ggcagcacta atatttgaat tccacgttta aatagtcgct cgaattttgc taattttttg
      721 aggttaatct gttctactgg atatttgccg cttaaactat ctcttacgga aaaaaatgct
      781 tgctcataat gggcactaac atactttaat ctgttcttct ctcgtctctt accgggaata
      841 cttacaatgc caattttaac aattctctta cgtgagaaca tattcaccaa aagttaagtt
      901 tctccctgca aaccatttaa cttaatgttt agaccaccgg cctctccgtg gttcaactac
      961 gttcaccaca agtcgtgtag gtcaaggtct tccccgcttt ctctcatgtt cagtccggag
     1021 accagaattc aagcaggttg ccgtccggat catagaaata gtaggcggtg gctttcatcc
     1081 acttaaaatg tttcggcccg taaacttcca ctcccttccc gcgcacgtgc tcaaccgcct
     1141 cggctaattt ttcgcggggc acgttgaaag cataatgcac ctgtccccag cgttggcctt
     1201 ccggcagcgg tgacttttcc gcaaagagca ggtccttatc ctgagttacc gcaaccgatt
     1261 gcggatggcc gggtacgcca gcccagaacc aggcccaatt cttatcggag gcggcggctt
     1321 cattgagcgt cagccccact acttcacggt agaaccgggc tgaggcagct acgtctttca
     1381 caaccaacac gatttccgac aaaccggtag tgaacactca ggctaccctt tcacgcaatc
     1441 gccaccggct tttctgaggt tcgacccacc ccaggcagga tcagttcccc gccggggcca
     1501 ttggttcaac acctctcttc ctccttctca tagtaactaa tgtaatgccg ccaataacgg
     1561 ccaccaaaag aattgccgca acaatccaca aaagcccttt cccgggcgga tgcatctcgg
     1621 ctttcagttc ttgttcatag gggttttcac tcaaaaggtc aactaaggca agtttccact
     1681 ccgccgtttg catccggtcg tctaagtttc cgttcgtgga aacaattttc ggcgcatgaa
     1741 gccgcacggt aaagtacctg ttcccaaagg cggtagaaat cgacgctttc gccatgaatt
     1801 cgccgaattt cagccagggg ttgcgttcgg atgtatccgc ctctgcttcc gtgctttctg
     1861 ccggggattc gccgaatttt tgccggaagg aaatattgcc gctgggcaac ttctctatgc
     1921 gaaagtcgga ttcttcgccg gaatcttcta tatagggggg aattttcttc tgaatttcgt
     1981 tgagattctg gaattgcctg accgtgacgt caagactaaa atggtgcatt cccccttcgc
     2041 tgtattcccg gaattcaacc cgctttacga aagaattctt ttctaagtcc tttttggctt
     2101 cctcgtaccg ctctcgcaca cgtccaataa gatcatcctg acttcgcccc tgagctaagc
     2161 caagcagtgc atcggaaatt atgaagtcct cttttatcct ccccgatccg tcggtactga
     2221 tccagatttc ctccgacatt tcaaagcagc cggagagaat aagacaggat gagagtagga
     2281 gagaaaacca aaccgacttc ttaaagcaga tttgtatcag cccaccactc ttcataactt
     2341 gccttacgca accactactg gcctttcggt cgtgtaggtt aaggtcttgt ctttcgccaa
     2401 gtagcgctca tttcccaacg gctcaaagac tttgcacttg gagagttccc cgccgtacac
     2461 gtgaatggtc acggctacgt ttctgctttc atttcccatt ctgtggtgct ccaccggcgg
     2521 aatgagcgcg ccggtctgcc ccttgccggg aaaaaggatt tctccggcgg tcatctgaac
     2581 tatgttttgg tctgtggttc gactccgctc accatcattt gcgagaggcc gcaaatgaaa
     2641 acgctccacc ttcatactcc cctggtacac cccctccacg caccaggtgc cggcgtggtc
     2701 gtgcaccggt gtggcctgcc ccgggctcca aaccatcacc acaatcgtaa atccaccgtt
     2761 cggctgcctg tataaaaggt ggcgtgcgta gtggtctttg gcgggctgga ggtactgcgg
     2821 cggcagttcg aggttgccct ctaagaagcg actcaagcaa tctttgactt tgggcacctg
     2881 cgcgtcgcct ttggcattag aacagatggc agtgagttct tcgatcaggt cgttaacggt
     2941 tgttccagct acggctttca ttcaggccct cccttcctct ggcggaaatc ataacgcgct
     3001 aaaagtaaac cgacaagccg aatttgacgg agccaaatga caccagtgga accccgcccc
     3061 tgtgctcggg attgaagcgc agggtcctcc gggtaacttc agtgcggtcc tccgtcgtct
     3121 gaccggcggt agtgacttta tagttcgtgg ttcgtttgaa ccattcatgt tccagagagt
     3181 tcacgtactc cgctaaaaaa
//
LOCUS       DBNGZD010000076         9395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1400567, whole genome shotgun sequence.
ACCESSION   DBNGZD010000076 DBNGZD010000000
VERSION     DBNGZD010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1400567"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1351
                     /locus_tag="AC16GM_02045"
     CDS             215..1351
                     /locus_tag="AC16GM_02045"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR2922486.1"
                     /translation="MRFETKVIHAGQEPDPATGAIATPIYQTSTYVQEGIGKHKGYEY
                     SRTQNPTRTALEKSLAALEGGKYALAFSSGMAAINTVLNLLRTGSHVISSDDVYGGTY
                     RIFEQVYRQYGLEFDYVNTSNISEIKKAIKSNTRLIWVETPTNPLLKISDLAEISRLT
                     KQRQLVLGVDNTFASPYFQQPLALGADIVVHSSTKFLGGHADVVGGAIITSNATFYER
                     LKFCQNAVGAVPGPLDCFLVLRGIKTLALRMRQQERNAEKVVKFLSRHPKVKKIYYPG
                     LKDHPGHLIAEIQMTGFSSMVSFEVSGGLEFARKVCENTQLFKLAESLGGVESLIEHP
                     ALMTHASVPKEVREEKGVSDTLVRLSVGIEHPDDLIADLKQALE"
     gene            complement(1443..2360)
                     /locus_tag="AC16GM_02050"
     CDS             complement(1443..2360)
                     /locus_tag="AC16GM_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922487.1"
                     /translation="MQLQVKSKLGLVVLAVTLVFFLFSGCSKSPTSPQTTQLTADQLA
                     VQNLVQTDPAFDVTAAMDESDSVSEGGLAKSAAVLDTVFHWWRQITDRDRHIDIMITD
                     STARVTVTDTLMGLLHVVGADSVGRVQFTTPLTDVATHKALFKRRHPPDGDEHRGWVL
                     AAVSDVLFKSSPLDTRQIRTTQVRTSMSGVNRTLTESGITALTPRDSILTFTIGDTVW
                     VTVTTGDPSDSVFLHVRDGHRDMRPHRKSLHNNGDGTFSGQFLVPQDLEGASGRKHMV
                     VDVIKHDVLDNHARYDSHGWGILYRVRNI"
     regulatory      2622..2729
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2938..3789
                     /locus_tag="AC16GM_02055"
     CDS             2938..3789
                     /locus_tag="AC16GM_02055"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015300426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HIR2922488.1"
                     /translation="MTQFARATESQITKAILDRFYQDFTNHLSTDVIIVGAGPSGLVA
                     GRDLAREGFKVLIVESNNYLGGGFWIGGYLMNGVTVRQPAQAILDEIGAPYQEVAPGL
                     YLSPGPHACSKLVAAACDAGVTVINMSKFDDVVLREGNRVAGVVVNWTPVSALPRAIT
                     CTDPVALEAKVVIDATGHDAVVCEALKRRKLLDYGTYGAMWVERSEDLVVEKTCEVHP
                     GLIVAGVAVATVFEIPRMGPTFGAMLLSGKKAAQIAKQLLPRKAAVSGNGQPKTGRTK
                     PALAGVK"
     gene            4013..4510
                     /locus_tag="AC16GM_02060"
     CDS             4013..4510
                     /locus_tag="AC16GM_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR2922489.1"
                     /translation="MQTLLVLTHCYPQNERDSAGIFVKDWVEMVRDGGYKVEVLVCEG
                     LAGKLTRWRGLLKAIGLLPSCFWSAFRKARAVDLVAAHWIFPSGLLAVVVGFLRRKPV
                     YVIMHAEPFVIEKRRWVRVIAKMILHIPAHVTVQVVSTPCRKIVEQIYRGRLVVAPMP
                     CRLIP"
     gene            4492..5118
                     /locus_tag="AC16GM_02065"
     CDS             4492..5118
                     /locus_tag="AC16GM_02065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2922490.1"
                     /translation="MPVDTLKVPIVLSVGRLVEGKGHMYLIRAMKMLRRETELIIVGT
                     GPERDRLIAEARTAANGSPPTFRFTLVENPTQEKLHTLYDQASVFVLPSVSDRRGWKE
                     ALGMVLLEAIAHRLPVVAFDSGGISDVIQDGVTGFLVKEGDEPGLANQIADALDGKLN
                     VETALRVTTAKFSRRKIQETVLKALAETLARNRAGGAAHQPARMPADR"
     gene            5222..6604
                     /gene="thiC"
                     /locus_tag="AC16GM_02070"
     CDS             5222..6604
                     /gene="thiC"
                     /locus_tag="AC16GM_02070"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR2922491.1"
                     /translation="MSVNGSCPEYCFSQMHYAKRGIITDAMRRVAEGEDQNPELIRAE
                     IARGRLIIPANVNHRSLVPMGIGIAVKCKINANIGNSAVISKIDDELAKLHLAVKFGA
                     DTVMDLSTGGNIDKIREAIIAASPVPVGTVPIYQAVAGVKKVEALTADDLVGMIEHQA
                     KQGVDYITVHAGVLREHLPLVQGRITGIVSRGGALLAQWIISHRQENPLYAHFDKILE
                     IARRYDVTLSLGDGLRPGCLADASDKAQFAELKTLGELTLRAWEKDVQVMIEGPGHIP
                     IDQLQMNVEMEQQLCHEAPFYTLGPLVTDIAPGYDHITSAIGAAMVGFYGASLLCYVT
                     PKEHLGLPEFEDVKQGVIAYKIAAHAADIARHRKGARERDDALSRARFAFDWERQFNL
                     SLDPETARKYHDLTMPHPVHKEAEFCSMCGPKFCSMHISKAVENWAVALLPAEESTPL
                     KELQESRPGT"
     gene            6611..6958
                     /locus_tag="AC16GM_02075"
     CDS             6611..6958
                     /locus_tag="AC16GM_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922492.1"
                     /translation="MTGKFAAQSFSILDRFVNKKKKGVHLFDIRVNYFADRDDYHFLV
                     PEKNTLHSSSNDLAFGKAAWSFHIAQKKFCSHRDLVDLKLFYPIDRLSPVIDYALCEQ
                     RKTCGVSNAGHVN"
     gene            7311..8768
                     /gene="dnaA"
                     /locus_tag="AC16GM_02080"
     CDS             7311..8768
                     /gene="dnaA"
                     /locus_tag="AC16GM_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR2922493.1"
                     /translation="MLKSEQEIWTDCLAYLEKRIKKQSFYTWLRPTRAERLMNDKLVV
                     SVPNRFVVEWLEEHYLPLIHEALLAVTGKSLAVILQIGANGGGAQLSESNGQVRSFAD
                     QRTAAQEPVHGESRSANSSASFHPKTIDPRVVKGAAPGDGQLNLRYTFDTFVVGESNQ
                     FAHAAALAVAESASGGAGQTKYNPLFVYGGTGLGKTHLAQAIGHYVKELTPQARILYV
                     TSEKFTSDFITAIGHNTANAFAAFYRQAELLIIDDIQFFTGKESTQMQFFHTFNALYH
                     NGKQIVLTADRAPKEIRGLEDRLLSRFSWGLVTDIQPPDMETRLAILKKRTAVEQIGL
                     PEDVLEFVALRVTSNIRELEGALVRLLAYASLARKDITLEVAQTVLKDTLSNGSAPSV
                     TIEQIKQEVAQEFGVPLEVLVAKRKTQEVALARQVAMYLARTLTSSSLKTVGEKFGGR
                     DHSTVLHACNVVAEQLKSNIDFKRRIDGVITSLYV"
     gene            9073..>9395
                     /locus_tag="AC16GM_02085"
     CDS             9073..>9395
                     /locus_tag="AC16GM_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR2922494.1"
                     /translation="MKFSIAKGKLLLGLQNVLSVVPTRSTLPVLSNILIEAQDGKLKL
                     SATDLEMSVTTILSANVTKKGVTTVPAKTFGDIIRELPEVQVEVSVTDTRMEIKAEKG
                     TYRLSG"
BASE COUNT         2146 a         2574 c         2675 g         2000 t
ORIGIN      
        1 cactgggcaa aggttagatt cgagcgacgc tggcggaaga gcaggtagaa aaagtaaatc
       61 cagtggtggc gcttaagtgc cgcctatgtg tttaggagag cgctttctcc caatcacgac
      121 ggttgcaaga atcccagctt ttcgcatatt cagttccacg ctatggttat cgatgcatga
      181 gaaatagcag aagaattatt ggagtgaggt tttaatgcgc tttgagacca aggtaatcca
      241 cgccgggcag gagcccgatc ccgccacggg cgccatcgcc acgccgatct accagacctc
      301 cacctacgtt caggagggga tcggcaagca caaagggtac gagtactccc gcacccagaa
      361 tcccacccgc accgctttgg agaaatcgct ggccgcactg gaaggcggca agtacgcact
      421 cgcgttttcc tccggtatgg cggccatcaa caccgtcctg aatctcttgc gcaccggcag
      481 ccacgtcatc tcctccgacg acgtttacgg cggcacgtac cgaattttcg agcaggtcta
      541 ccgccagtac gggctggagt tcgactacgt taatacctcg aacatctccg agattaaaaa
      601 ggcaattaaa tcgaataccc gtttgatctg ggtcgaaacg ccgaccaacc cgcttttgaa
      661 aatctccgac ctggctgaaa tttcccgcct gaccaagcag cgtcaactgg ttttaggggt
      721 cgataacacc ttcgcctcgc cttacttcca gcagccgttg gctctcgggg ctgacatcgt
      781 cgtccacagt tcgaccaaat tcctgggcgg ccatgccgac gtggtgggcg gcgccatcat
      841 cacctccaat gccactttct atgagcggct gaagttctgc cagaatgcgg tgggcgcggt
      901 gccggggcct ctggactgct ttctcgtttt gcgcggcatc aaaactctcg ctctccggat
      961 gcgccagcag gaacgcaacg cggagaaggt ggtgaaattc ctctcccggc acccgaaggt
     1021 gaaaaaaatc tactatcccg gattgaaaga ccaccccgga catctgatcg ccgaaatcca
     1081 gatgaccggc ttcagctcga tggtctcttt cgaagtgtcc ggcggcctgg agtttgcccg
     1141 caaggtctgc gagaacaccc aattgttcaa attggccgag agtctgggcg gggtggagtc
     1201 gttgatcgag cacccggctt tgatgaccca cgcttccgtt cccaaagaag tgcgggaaga
     1261 aaaaggggtg tccgatacgt tggtccgcct ctcggtgggc attgaacacc cggacgattt
     1321 gatagccgac ctcaagcagg cgctggagta aaaacggtca aacaccggac tgcgggaagc
     1381 gaaacgggac acacactggt gtgtgtcccg tttcggcttg ccccgcttga cgctagcgcg
     1441 gcttagatat tccggacgcg gtagaggatt ccccacccgt ggctatcgta gcgcgcgtgg
     1501 ttatcgagga cgtcgtgctt gatcacgtcc accaccatgt gcttgcggcc cgaggcaccc
     1561 tccaggtcct ggggaaccag gaactgtccg ctgaaagtgc cgtccccgtt gttgtggagc
     1621 gacttccggt gcggacgcat atcccggtgt ccgtcgcgca catgcagaaa aaccgaatcg
     1681 ctggggtcac cagtggtaac cgtgacccag accgtgtcgc cgatggtaaa agtcaagatt
     1741 gagtcgcgcg gggtcagcgc ggtaatgcca ctttccgtta gggtccggtt cacgccgctc
     1801 atactggtgc gaacctgggt tgtgcgaatc tgacgcgtat cgagcgggct ggatttaaac
     1861 agaacgtcgg aaaccgccgc gagcacccag ccgcggtgct cgtcaccatc cggggggtga
     1921 cgccgcttga agagggcctt atgggtcgcc acgtccgtga ggggggtggt aaattgcacc
     1981 ctgccgacgg aatcggcacc caccacgtgg agcagtccca tcaaggtgtc ggtgacggta
     2041 accctggcgg ttgaatcggt aatcatgatg tcgatgtggc ggtcgcggtc ggttatttgc
     2101 cgccaccagt ggaatacagt gtccagcacg gcggcacttt tggccaggcc gccttcgctg
     2161 acagaatcgc tttcatccat cgcggcggta acatcgaacg ccggatctgt ctgcactaaa
     2221 ttctgtacgg ccagctgatc ggccgtgagc tgcgtggttt ggggcgatgt cggacttttg
     2281 ctgcagccgg agaacaggaa gaacaccaac gtgaccgcca gtaccaccaa cccgagtttg
     2341 gacttcacct gcaactgcat tgttggttac ctccttacga cttcttttcg actgaacgac
     2401 gtacttaccg tggccttaag cctcgccagg tgcatcggta cctccttcct ttcaccccaa
     2461 ttcgcggcgt ttggccaaaa tgcctgctct gactttcaaa agggataaac gcccattgcc
     2521 cgggaatgtt tgcaggattt tcggggactg gggcgcggtg caatcctaac cctaaaaagt
     2581 gcttgacaag tttccgaaag ctacgtatat agttgaatag aaaacaaaac caggggtggt
     2641 tttcccgccg cgggcggagg aagcctgaga taataccctt agaacctgaa ctgggtaatg
     2701 ccagcgtagg gagcgatgat ggaagaccgc accacagtat tctgcaggac cctccggttg
     2761 cggccttttt attttcgggt caaccccgcc atatccgggg cctgacaacc ctccagttcg
     2821 gtgacctccc ttcgggggcg ccggacatcc atcgtcaaac tcgcgctggc attgccctga
     2881 agacgcagtc ccgccaaaaa cgggatctcg tagtaaacct gaaggaggcc tgaaaaaatg
     2941 acacaattcg cacgggcaac cgaaagccag atcaccaagg cgattctcga ccgcttctac
     3001 caagatttca ccaaccacct ttcgaccgac gtgatcatcg tcggggctgg accctcggga
     3061 ctggtagcag gacgggattt ggcccgggag ggtttcaaag tgctgatcgt ggagagcaac
     3121 aactacttgg gcggcgggtt ctggatcggc ggctacctga tgaacggggt gaccgtccgc
     3181 cagccggcgc aagcgattct ggacgaaatc ggcgcgccgt accaggaggt cgcacccggt
     3241 ctctaccttt cgcccggacc gcacgcctgt tcgaaactcg tcgctgccgc ctgcgacgcc
     3301 ggggtgacgg tgatcaatat gtcaaaattc gacgatgtgg tgctgcggga agggaatcgg
     3361 gtggccggcg tggtggtgaa ctggacgccg gtttcggcgc tgccccgcgc catcacctgt
     3421 accgatccgg tggccctgga agccaaggtc gtcatcgacg ccaccggcca cgatgcggtg
     3481 gtctgcgagg cgctcaagcg gcgcaagttg ttggactacg gtacctacgg cgcgatgtgg
     3541 gtggagcggt cggaagattt agtggtcgag aaaacctgcg aagtgcaccc ggggttgatc
     3601 gtcgccggcg tggcggttgc caccgtgttc gaaatcccgc ggatggggcc gactttcggg
     3661 gcgatgctcc tctccggcaa aaaagcggcg cagatagcca aacagttgct cccccggaag
     3721 gcggcggtct ccggcaacgg ccagccaaag actggcagga ctaagccggc tctggccgga
     3781 gtcaagtaag ggaagcactg aaaagaaggc ggagaacttc ccagcaacca ggggtggcaa
     3841 cgccgccgcc ccgccacatt ggttactgcg ggagttcatc aatctcgttc cggcgtacat
     3901 ccttagccgc ttgaaaacgg agaacaacgc cgcgtggaca gaaaaacgag ttcactttat
     3961 actgcaggac aacgtgtgga tttacccttt gttcgcttgc cgattgtagc gtatgcaaac
     4021 cctcttggtc ctcacgcact gctaccccca aaatgagcgc gacagtgccg gaatattcgt
     4081 caaggattgg gtggaaatgg taagagacgg ggggtacaaa gtcgaagtgc ttgtgtgtga
     4141 gggattggcg ggcaagttga cccgttggcg gggcctgctc aaggccatcg gcctgctccc
     4201 ctcctgcttc tggtctgctt tcagaaaagc gcgggctgtc gatttagtgg ccgcccactg
     4261 gatttttccg tccggtcttc tggcggtggt cgtcggcttc ctccggcgca aaccggtgta
     4321 tgtcatcatg cacgccgaac cgttcgtcat cgaaaagcgg cgctgggtac gcgtcatcgc
     4381 gaaaatgatt ctccatatcc cggcgcacgt cacggtgcag gtagtctcca ccccctgccg
     4441 caaaatcgtc gaacaaatct accggggccg cctcgtcgtc gctccgatgc catgccggtt
     4501 gataccctga aggtgccaat tgtcctctcc gtgggccggt tggtcgaagg caagggccat
     4561 atgtacctga ttcgcgccat gaagatgctg cggcgagaga ctgaacttat tatcgtcggt
     4621 accgggccgg agcgggaccg cctgatcgcc gaagcgagaa ctgcggcgaa cggttcgccg
     4681 ccgacgtttc ggtttaccct ggtggaaaac ccgacgcagg aaaaactaca taccctctac
     4741 gatcaagcct cggttttcgt cctgccgagc gtcagtgacc ggcgcgggtg gaaggaggct
     4801 ctgggcatgg tgctcctgga ggccatagcc caccgcctgc ccgtggtggc gtttgactcg
     4861 ggcggcatct ccgacgtcat ccaggacggc gtaacgggct tcctggtcaa ggagggcgat
     4921 gaacccggac tggccaacca gattgccgat gcgctggacg gcaaattgaa cgtggaaacg
     4981 gcgctgcggg taacgacggc caaattctcg cgccgaaaaa ttcaagagac cgttctaaaa
     5041 gcactggccg aaactctggc gcgaaatcgg gcaggcggtg cagcgcacca gccagcccgg
     5101 atgcctgcag acagataatc gacgtagggc gtggaaccat gaacccgata aaaaaaccac
     5161 gccgcggtat aaagaaagcg tgccctgcta tcggcggggc ctgtcgcgag aggtctccgt
     5221 tatgtcagta aatggatcgt gcccggagta ttgctttagc caaatgcact atgccaagcg
     5281 ggggattata accgacgcca tgcggagagt ggcggaagga gaggaccaaa atccggaact
     5341 aatccgcgcc gaaatcgccc gcggccggct gatcattccg gccaacgtca accaccggag
     5401 tctggtgccg atgggaatcg gcattgccgt caagtgcaag atcaacgcca acatcgggaa
     5461 ctcggcggtc atctcaaaaa ttgacgacga gctggccaag ttgcacctgg cggtcaaatt
     5521 cggcgccgac acggtaatgg acctctccac cggtgggaac attgacaaaa ttcgggaggc
     5581 gattattgca gccagtccgg ttccggtggg aacggtgccg atttatcagg ccgtggcggg
     5641 cgtcaaaaaa gtcgaagccc tgaccgccga cgacttggtc gggatgatcg agcaccaggc
     5701 caaacaggga gtcgattaca ttacggtcca cgccggagtc ctgcgggaac acctgccttt
     5761 ggtgcagggg cggattaccg gcatcgtcag ccggggcgga gcacttctgg cacaatggat
     5821 tatctcacat cgccaagaga atccgttgta tgcgcacttc gacaagattc tggagattgc
     5881 ccggcggtac gacgtcacct tgagtttagg cgacggcctc cggcccggct gcctcgccga
     5941 tgcttccgac aaagcccaat tcgccgaact gaaaacgctc ggggaattga ccctgcgggc
     6001 ctgggagaaa gatgtgcagg tgatgatcga gggaccgggg cacattccga ttgaccagtt
     6061 gcagatgaac gtggaaatgg aacagcaact gtgccatgag gcccctttct acacgctggg
     6121 gcctctggtc accgacatcg caccggggta tgaccacatt acttccgcca tcggcgcggc
     6181 tatggtggga ttttacggtg ccagcctcct ctgctacgtt acccccaagg aacacttagg
     6241 gttgccggaa ttcgaagatg tgaagcaagg ggtaattgcc tacaaaattg cggcccacgc
     6301 cgccgacata gcccggcacc ggaagggagc gcgggaaaga gacgacgctc tgtcgcgggc
     6361 ccggtttgct ttcgactggg aacggcagtt taatctttct ttggatccgg agaccgcccg
     6421 gaagtatcac gacctgacga tgccccaccc cgttcacaaa gaggccgaat tctgctccat
     6481 gtgcgggccg aagttttgct cgatgcacat ttccaaggcg gttgaaaact gggcggtggc
     6541 gctgctgccg gcggaggaat caacgcccct gaaagagctg caggaatcgc ggcccgggac
     6601 ctaaaaacgt ttgaccggca agttcgcggc ccaaagtttc tcgatccttg atcgctttgt
     6661 gaacaaaaag aagaaggggg tacatctttt cgatatccgg gtaaattatt ttgcggatcg
     6721 ggatgattat cattttcttg ttccagagaa aaacactttg cactcctcgt caaatgatct
     6781 ggcgttcggg aaggcggctt ggagttttca cattgcccag aaaaaattct gctctcatag
     6841 agacttagtc gacttgaaat tgttttatcc aatcgatcga ctttccccgg tgatcgacta
     6901 cgcactatgc gaacaaagaa aaacatgcgg cgtaagtaat gcggggcacg tcaattaact
     6961 tttttgtgcg aacaagaaga aaaactcttg acagcatcgc tcatcacgag ttatttacca
     7021 actcaacaac tcgtgcttat taccgaacaa ggaaaaatcg ttggatctga ttgggtgggc
     7081 gggaacttat tgttctgaca ggtaattttc ctttgtgggc aaggcccttt gctgtggaga
     7141 atgggtggaa aagtgttttg atttttttat taacggctta ccgctcttcg agttagcttt
     7201 ttccacaacg gcttgaccga aatgactttc agtttcttga gtgctccaaa tgcgagtcgg
     7261 tgtgcaaata aatgattgct caaagttttc ccccattgtc tgtggaaaaa atgttgaaaa
     7321 gtgaacagga aatttggacg gattgcctcg cttatttaga aaagcggatc aaaaagcaga
     7381 gcttctacac ctggctgcgg ccaaccaggg ccgagcgtct gatgaacgac aaattggtag
     7441 tgtctgttcc caatcgcttt gtggttgagt ggttggaaga acactacctc ccgttgatcc
     7501 acgaggcgct gttggcggta accggcaagt cgctcgctgt gattttgcag ataggtgcga
     7561 atggcggggg tgcacaatta tctgaatcga atggtcaagt gcggtctttt gcagaccaac
     7621 ggactgccgc tcaagaaccg gttcacggcg aatcgcgaag cgcaaactcg tcggcgagtt
     7681 ttcatccgaa aactatcgat ccccgggttg tgaaaggcgc cgccccaggt gacggacaac
     7741 ttaacttgcg gtacactttc gatacgttcg tcgttggtga atcgaaccag tttgcgcatg
     7801 ctgccgctct ggccgtcgcg gaatccgctt ccggcggagc gggacagacc aaatacaatc
     7861 cgcttttcgt ttatggcggc acgggtctgg ggaagaccca tttggcgcaa gccattgggc
     7921 actatgtcaa ggagctgacg ccccaggccc ggatacttta cgtcacctct gaaaaattta
     7981 ccagcgactt cattaccgcc atcggccata acaccgcgaa tgccttcgcc gcgttttacc
     8041 gccaagccga acttttaatc atcgatgata tccaattttt caccggcaaa gaatcgaccc
     8101 agatgcagtt cttccatacc ttcaacgccc tgtaccacaa cgggaaacaa attgtcttga
     8161 ccgccgaccg ggctccgaag gagattcggg ggcttgagga tcgcctgctt tcccgcttct
     8221 cctggggttt agtgacggac attcagccac cggatatgga gaccagactc gcgatcctca
     8281 aaaaaagaac tgccgtggaa cagatcggcc ttccggaaga tgtgctggaa tttgtagctc
     8341 tgcgggtaac ttccaacatc cgcgaattgg aaggggcttt agtccggctt ttagcgtatg
     8401 cttccctggc gcgtaaagac attacccttg aggtagcgca aaccgtcctc aaagacactc
     8461 tctccaacgg atcggccccg tcggtaacga tcgaacagat aaaacaggag gtggcccaag
     8521 aatttggggt tcccttagag gtgctggttg ccaaaagaaa aacgcaggaa gtcgccctgg
     8581 cccgacaggt cgcaatgtac ttggcccgga cactgaccag tagttcgctc aaaacggtcg
     8641 gtgagaaatt cggcgggcgt gaccactcaa cggtactcca cgcctgcaac gtcgtcgctg
     8701 aacaactcaa gagcaacatc gacttcaaac ggagaattga cggcgttatc acctcactct
     8761 atgtgtagaa tggggggata acaggtttaa tcccgacgca taaccgtcct gttgtggata
     8821 cctctcattt tcggaattgg ttaccccgac agccttcaca ccacaaattc cttctggcaa
     8881 ggactatcct ctttcgagag aaagaattga aacggcatct caaaagtacc cacatttcca
     8941 cgctacttgc ctttatgatt atattttata aattcatact ctatagatta ctactgtgga
     9001 cgaaaatgac gcgtgaaagc tcttgaccct gaccggtaac ggggttctcc gtctgcccgt
     9061 cgaggaggga aaatgaaatt tagcatagcc aaagggaaat tactgcttgg gttgcagaat
     9121 gttttaagcg ttgtgccgac gcggtctacc ttaccggtac tttccaatat cctcattgaa
     9181 gctcaagatg gaaaattgaa actttcagcg actgatctgg aaatgtccgt gacgaccatt
     9241 ctttctgcca atgtgacgaa gaaaggggta acgaccgttc ccgctaaaac attcggtgac
     9301 atcatccgtg aattgccgga ggttcaagtt gaagtttcgg tgaccgatac ccgcatggaa
     9361 attaaggccg aaaagggaac gtaccggctc tccgg
//
LOCUS       DBNGZD010000077         6733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_635607, whole genome shotgun sequence.
ACCESSION   DBNGZD010000077 DBNGZD010000000
VERSION     DBNGZD010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_635607"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="AC16GM_02090"
     CDS             <1..253
                     /locus_tag="AC16GM_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR2922495.1"
                     /translation="LRLKVRVEEIQAVPSVTGVWSNVNWHEREVFDMFGLKFTGHPDL
                     RRILMPDDWVGHPLRKDFPLTYEAPKFSHNKDLPPEIVK"
     gene            336..1508
                     /gene="nuoD"
                     /locus_tag="AC16GM_02095"
     CDS             336..1508
                     /gene="nuoD"
                     /locus_tag="AC16GM_02095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR2922496.1"
                     /translation="MLKTETMTLNMGPQHPAPHGVLRVILELDGETVIKATPEIGYLH
                     TGIEKNAEDKKYYTALPMTDRMDYAAPMSNNLGFCLAVEKLIGMEIPPKAQYLRVLLT
                     ELTRIKSHLLWIGTHAIDIGAMSMFLYAFRERERVIEIYEMVSGQRMMSTYFRVGGVV
                     QDVPPDFEPKIRAILRDFPKRFDEYENLLTNNPIWRNRTIGVGVIAAKDAIDAGLSGP
                     SLRGCGVKWDIRKSNPYSGYEKFDFEIPTGKNGDVYDRYLVRIAEMRQSLRIIRQALD
                     GMPEGDVMSRMPGFTPPPKREVLYSMEALIFQFKYWTEGFRPPVGEVYQAIESPKGEL
                     GFYFVSDGSPKPYRMHVRAPSFVNLSALSKLVEGRLVADVVATIGSIDIVLGEIDR"
     gene            1508..2074
                     /locus_tag="AC16GM_02100"
     CDS             1508..2074
                     /locus_tag="AC16GM_02100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR2922497.1"
                     /translation="MAVATPEKKPGKTGPSPGIKLLLPQTIQKMRQALPKFPVRRSAI
                     LPALHAAYDQLGYLNDEIYAEISQVIGVPALEIVEAATFYTLFPKKKVGKYLIMVCTN
                     ISCALCGADSLVSYLEQKLDIKNGETTPDGLFTLWTVECLGSCGTAPMLQVNEAFYEN
                     LTHEKVDRLIEAWRQGKPVEVKIMERGW"
     gene            2252..3535
                     /gene="nuoF"
                     /locus_tag="AC16GM_02105"
     CDS             2252..3535
                     /gene="nuoF"
                     /locus_tag="AC16GM_02105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR2922498.1"
                     /translation="MPTYDKVLFKYIDDPGQYRVDTYIKHGGYVALAKALKMAPDAVT
                     AEVKKSNLRGRGGAGFPTGLKWSFVPKKPGPKYLLCNADESEPGTFKDRELMEKDPHQ
                     VLEGCIIASYAIGANTCYIYIRGEFVFAARRLEEAIAEAYAKGFLGKNILGSGFDLEM
                     HLYRGGGAYICGEETGLIESLEGKRGFPRVKPPFPASEGVYRAPTVVNNVETLANVPH
                     IINRGADWFLSIGPSKSPGTKIFCLSGHVNRPGNYELPMGVPLEELIYEYGGGIRHGR
                     KLKAVIPGGSSVPVLPADKVSCPLDFESVAAAGSALGCAAVIVIDDSTCMVWTAERLA
                     RFYEHESCGKCTPCREGTFWMREILHRIETGAGKREDIDTLLDMADNITFKTVCALAD
                     GAAAPTISTIQHFREEYEEHIRLKRCPMPVSFELA"
     gene            3657..6332
                     /gene="nuoG"
                     /locus_tag="AC16GM_02110"
     CDS             3657..6332
                     /gene="nuoG"
                     /locus_tag="AC16GM_02110"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR2922499.1"
                     /translation="MSDKTFTITVDGKKIEARPGETILEAALRSGIQIPTFCWHPKLD
                     PVGACRICLVKVEKSPKLVVSCATPAVAGQVVSTNSPEVIKGRQGVLEFLLINHPLDC
                     PTCDKGGECDLQDLTFRYGVDLSRYLEHKFRWAEQNTFDEISLGPMVLRNQNRCIHCY
                     KCTRVIQEVCGEHDLGAYQRGYRTEIHHWPAKAFEDQYAGNAVEVCPVGALTAKDFRY
                     KVRVWLTKQTKSVCQHCSDGCNTLLWVDPERNFLSRVTSRRNDCVDEGFLCSRGKFQY
                     QEANSPERLKTPLIRKDGRLVEVTWDEAYATLAKRLKDLKAKNGGESLAGIASATTPN
                     EDLYLFSRFLRTLVGSNNLDFRVNWGTNLPDPDLFYKFNRFSVAGVEQKKMIFVFGCD
                     FNREHPIITLRIRKASRKFGARVVLANPYRTKLGDSADVEAVYRFGSEVTFVNSLCQE
                     IIRQKLYPKEGYFCGEAIPEDFIQHLTGFPADQTAKVTGVDSEQVERLARALAGQANM
                     AILCGNGVLQHPQRNELVAALVNLAALLIGPSKVAQSLWLLPEEANFQGALDTGFLPD
                     QLPGNQPLADSQARAKCEQAWKKSISQSAGLSISQIIEATKSGKIKTWWVIGGEPTWQ
                     YPDHAAVEEAVKKVECLIVQANFRTPVVERADIVLPSAMLFERDGSFTSAEKRVQRFR
                     AGMKPVGQAKADWQIFSELAWWLEEPFDLFSAEEVFDEMAKLVPQYAGLNCKLLGDQG
                     LRWAEVNPEKEWTHKFTKVSADPIVAPPGDYPFLLLSGNSLYQSGFVYARTENLLRQE
                     PHPTVELSSETLASFACEEGDWVEIASPSGKVRVTAKENPYLSADVGIFHDNYPEARF
                     NFLTDESRDFCRVKLSKAPQPVTAQVPEPALAGVR"
     gene            6329..>6733
                     /locus_tag="AC16GM_02115"
     CDS             6329..>6733
                     /locus_tag="AC16GM_02115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HIR2922500.1"
                     /translation="MSNPVFIDALITVIKVFIVLNVILVSCAYLTLMERKVLARMQVR
                     LGPNKVGPGGIFQPFADLIKLIVKEDIVVTAGNRVLYILAPLLSWIPAFITFAVIPFG
                     TSVTIFGHKVDLVISDFNIGILYIFAATALGVY"
BASE COUNT         1485 a         1848 c         2013 g         1387 t
ORIGIN      
        1 gctccgcttg aaagtgcgcg tcgaagaaat acaggcggtg ccgtcggtca ccggggtctg
       61 gtcaaacgtc aactggcacg agcgggaggt cttcgatatg ttcggcctca aattcaccgg
      121 ccaccccgac ctgcggcgca tcttgatgcc cgacgactgg gtggggcacc ctttgcggaa
      181 agactttccc ttgacctacg aagcgccaaa gttttctcac aacaaagact taccgcctga
      241 aatcgtgaaa taaccttgtt ggaaaattgc cggacatata aattgcgcgc tcgtttgtga
      301 caaaaggtac aagccaaaac tgattcgcct ccactatgct taaaaccgaa acgatgacgc
      361 tcaacatggg gccccagcac cccgccccgc acggggtcct gcgggtcatt ctggagctgg
      421 acggtgaaac ggtgatcaaa gcgacgcctg agatcggcta cctgcacacc ggcatcgaaa
      481 agaacgccga ggacaagaag tactacaccg ccctgccgat gaccgaccgg atggactacg
      541 cggcgccgat gtccaacaac ttggggttct gtctggcggt ggagaagttg atcgggatgg
      601 agattccgcc caaggcgcag tacctgcggg tgcttttgac ggaactgacg cgcatcaaga
      661 gccacttact ctggatcggc acgcacgcca tcgacatcgg cgccatgagc atgtttttgt
      721 acgcctttcg cgagcgggag cgggtcattg aaatttacga gatggtctcc ggccaaagga
      781 tgatgtcgac ctacttccgg gtgggcgggg tggtgcagga cgttccgccc gactttgagc
      841 cgaagatccg cgccatcctc cgagattttc cgaagcggtt tgacgagtac gaaaatctcc
      901 tgacgaacaa ccccatctgg aggaatcgga cgattggcgt tggtgttatt gcggcgaaag
      961 atgcgatcga cgccggcctc tccggcccca gcctgcgcgg ctgcggcgtg aagtgggaca
     1021 tccgcaagtc gaacccctac tccggctacg agaagttcga ttttgaaatc ccgaccggca
     1081 agaacggcga tgtctacgac cggtatctgg tgcgcatcgc cgaaatgcga cagagtttgc
     1141 ggattatccg gcaggcgttg gatgggatgc cggagggtga cgtaatgtcc cggatgccgg
     1201 gattcacccc gccgcccaag cgggaagtgc tctacagcat ggaagccttg atcttccagt
     1261 tcaagtactg gaccgaagga ttccgaccgc cggtgggaga agtctaccag gccatcgaat
     1321 cacccaaagg agaactggga ttctacttcg tctccgacgg gagcccgaag ccctaccgga
     1381 tgcacgtgcg cgcgccgtcg ttcgtgaatt tgagcgcctt atccaaatta gtcgaaggcc
     1441 gcctggtggc tgacgtggtg gccacgatcg gaagcatcga catcgtcttg ggggagattg
     1501 accgctgatg gctgtcgcga cgcctgaaaa aaagccggga aagacgggcc cttcgcccgg
     1561 aatcaaactt ctcttgccgc agacgattca aaaaatgcgg caggcgctcc cgaaatttcc
     1621 ggtgcggcgg tcagcgattc tcccggcact ccatgccgcc tacgatcagc tcggctactt
     1681 aaatgatgag atttatgcgg aaatcagtca ggtcatcggc gtaccggctt tggaaattgt
     1741 cgaagcggcc actttctaca ccctcttccc gaagaagaaa gtcggcaaat atctgatcat
     1801 ggtctgcacg aacatttcct gcgcgctctg cggagcggat agtctcgttt cttacctcga
     1861 acagaagtta gacatcaaaa acggggagac gacaccagac ggcctattca cactctggac
     1921 agtggagtgc ctgggctcct gcggcaccgc gccgatgttg caggtcaatg aggccttcta
     1981 cgagaatttg acgcacgaaa aggtcgaccg cctcatcgaa gcctggcgac aggggaaacc
     2041 ggtggaagtg aagataatgg aacggggatg gtagtttacc cgggaggggc gacttcagtc
     2101 gcccgaaagc atcgcccgga atttttacac agaaccgggc tttagcccgg ttctggagaa
     2161 aaaatagcaa tggggagcgc gcgactggtt cggcttcgct caccacaggt aaagtcgcgc
     2221 ctcccgttgc ggtaacgaag ccgttacgat aatgccaacg tacgacaaag ttcttttcaa
     2281 atacatcgac gacccggggc aataccgggt tgacacctat atcaagcacg gtggttacgt
     2341 cgcgttagcc aaggcgctga agatggcgcc ggatgcagtc accgccgagg tgaagaaatc
     2401 gaatctccgc ggccgggggg gggcggggtt tcccaccggg ctcaagtgga gtttcgtgcc
     2461 gaagaagccc gggccgaagt acctcttgtg caacgccgac gagagcgagc cggggacctt
     2521 caaagaccgg gaattgatgg agaaagaccc gcatcaggtg ctcgagggat gcatcatcgc
     2581 ctcctacgcc atcggagcca atacctgcta catctacatc cggggcgaat ttgttttcgc
     2641 cgcccgccgg ttagaggagg caatagcgga ggcgtacgcg aagggatttt tgggcaaaaa
     2701 tattctgggt tccggttttg acctggaaat gcacctctac cggggagggg gagcgtacat
     2761 ctgcggggag gagacggggc tgatcgaatc gttggaaggg aaacgcggtt tcccccgggt
     2821 gaagccgccc tttccggcga gcgaaggggt gtacagagcg cccacagtgg tgaacaacgt
     2881 cgaaaccttg gccaatgtgc cgcacatcat caaccggggc gcggactggt ttttgtccat
     2941 cggaccgtcc aaaagtcccg gcacaaaaat cttctgcctc tccggccacg tgaaccggcc
     3001 cggaaactac gaattgccga tgggcgtgcc gctggaggag ttgatttacg aatacggtgg
     3061 gggaatccgc cacgggcgga agttgaaggc ggtgattccc ggcgggtcgt cagtgccggt
     3121 tttgccggcg gataaagtca gctgcccact cgacttcgaa tcggtggctg cggccgggtc
     3181 agccttgggc tgcgcagccg ttatcgtgat cgacgactct acctgcatgg tctggacggc
     3241 ggagcggctg gcccgattct atgaacatga atcgtgcggc aagtgcaccc cctgccgcga
     3301 aggaacgttc tggatgcggg agattttgca ccggattgaa accggagcgg ggaagcggga
     3361 ggatatcgat acgcttttgg acatggcgga caacatcacc ttcaaaacag tttgcgctct
     3421 ggcggatggt gcggcggcgc cgaccatttc gacgattcag cattttcggg aggagtacga
     3481 ggagcatatc cggctgaaac ggtgcccaat gcccgtgagt tttgagttgg cgtaatgatt
     3541 ggaaacgcgg atcagattcg gctcagggaa gggcaaggcc tgaatgaccc ctcccggctc
     3601 tcccctctaa gcggagggaa aagcgtccga agggtagaac ttttgacgtg acaagcatga
     3661 gcgataaaac gttcaccatc accgtagacg gcaagaagat cgaagccagg ccgggcgaga
     3721 cgattttgga agcggcgctg cgatctggca tccagattcc caccttctgc tggcacccga
     3781 aactcgaccc ggtgggggcc tgccggattt gcctggtgaa ggttgaaaag tcgccgaagt
     3841 tagtggtttc ctgcgctacg ccggcggtgg cgggacaggt tgtgtccact aattccccgg
     3901 aggtaatcaa gggccgccag ggggtgttag agtttttact catcaatcat ccgctggact
     3961 gtcccacctg cgacaagggc ggcgagtgcg atctgcagga tttgaccttt cgctacggcg
     4021 tcgatctctc gcgctacctc gaacacaaat tccgctgggc ggagcagaat acgttcgacg
     4081 aaatttccct gggcccgatg gtgctccgca accagaaccg ctgcatccac tgctacaagt
     4141 gcacccgggt gattcaggaa gtctgcgggg agcacgactt gggcgcctac cagcgcggct
     4201 accggaccga aatccaccac tggccggcca aggcgttcga agatcagtac gcggggaacg
     4261 cggtggaggt ctgcccggtc ggtgctttga ccgccaaaga ttttcgctac aaggtcagag
     4321 tctggctcac gaagcaaacg aaatcggtct gccagcactg ttcggacggc tgcaacaccc
     4381 tgctctgggt cgatccggag cgaaacttcc tttctcgggt cacctcccgg cgcaacgact
     4441 gcgtggacga agggtttttg tgcagccggg gaaagtttca gtaccaggaa gccaactcac
     4501 cggagcgcct gaaaacacct ttgatccgca aagatggccg cctggtcgaa gtaacctggg
     4561 atgaggcgta tgcaacgctg gccaaacgtc tgaaggactt gaaagcgaaa aacggcggag
     4621 agagtcttgc cgggattgct tcggctacga cgccgaatga agacctctac ctgttcagcc
     4681 gcttcctgcg cacgctggtc ggctcgaaca atcttgattt ccgggtcaac tggggtacaa
     4741 acctgcctga tcctgattta ttctacaagt ttaaccgctt ttccgtcgct ggtgtggaac
     4801 agaaaaaaat gattttcgtt ttcggctgtg atttcaaccg cgagcacccg atcatcacgc
     4861 tccgcatccg caaggcctcc cgcaagttcg gtgcgagggt cgttctggcg aatccctacc
     4921 gcacgaaatt gggcgacagt gccgacgtgg aggcagtcta ccgcttcggt tctgaagtaa
     4981 cgtttgtcaa ttcgctttgc caggaaatca ttcggcagaa actctacccg aaagaaggct
     5041 atttctgtgg tgaggccatt cccgaagatt tcattcagca tttgaccggt tttccggcgg
     5101 accaaaccgc aaaagttacc ggcgttgatt cggaacaagt tgagcggtta gcacgggctc
     5161 tggccggcca agccaacatg gccatcctct gtggtaacgg cgtacttcag catccccagc
     5221 ggaacgaatt ggtcgctgcg ctggtcaatc ttgcggcact gctgatcggg ccgtccaagg
     5281 ttgcccagtc gctctggtta ttgccagaag aagccaactt ccagggagcg ctggataccg
     5341 gttttctgcc cgaccaattg cccggcaacc agccgcttgc cgactcgcaa gcgagagcga
     5401 agtgtgaaca agcctggaaa aagtcgatct cccagagcgc tggtttgagc atttctcaga
     5461 ttatcgaagc gacgaaatcc gggaaaatca aaacctggtg ggtgattggc ggcgagccga
     5521 cctggcagta cccggaccac gcggcggtgg aagaggcggt caaaaaagtt gaatgcctca
     5581 tcgtgcaggc gaatttcaga acgccggtgg tggagcgggc ggacattgtt ttgccctcgg
     5641 cgatgctttt tgagcgcgat ggttccttca ccagcgccga gaagcgggtg cagcggttcc
     5701 gcgcaggaat gaaaccggtg ggccaggcca aagccgactg gcagattttt tctgaattag
     5761 cctggtggct ggaggagccc tttgacctat tttccgccga agaagttttt gatgaaatgg
     5821 ccaaacttgt tccgcaatac gctggtttga actgcaaact cctcggcgat cagggactgc
     5881 gctgggcgga ggtcaacccg gaaaaagagt ggacgcacaa gttcacaaag gtgtctgccg
     5941 atcccattgt cgcaccgccg ggagattatc ctttcctctt gctctccggc aattcgctct
     6001 accagtcggg cttcgtttat gcgcgaacgg aaaatctcct aaggcaggag ccgcatccca
     6061 ccgttgaact ttcgtccgag accttggcgt ctttcgcttg tgaagagggt gattgggtgg
     6121 agatcgcttc gccctccgga aaggtgcggg tgacggccaa agaaaatcct tacctttccg
     6181 ccgacgtggg aattttccac gacaactatc cggaagcccg gtttaatttc ttgaccgatg
     6241 aaagccggga tttctgccgg gtgaagttga gcaaagcgcc gcagccggtc accgcgcagg
     6301 tgccggaacc ggcgctggcg ggagtgcgat gagcaatccg gtgttcatcg acgccctcat
     6361 cacggtcatc aaggtgttca tcgtcttgaa cgtgatctta gtttcttgcg cttacctcac
     6421 tctgatggaa cggaaggttt tggcccggat gcaggtacgc ctggggccca ataaagtcgg
     6481 ccccggcggg attttccagc ccttcgccga tctgattaaa ctgatcgtca aagaagacat
     6541 cgtcgtgacg gcgggcaacc gggtgctcta catcttggcg ccgcttttgt cctggattcc
     6601 ggccttcatc acctttgcgg tgattccctt cgggacatca gttaccattt tcggccacaa
     6661 agttgacctg gtgatttccg acttcaacat cggcatcctc tacatctttg cggccacggc
     6721 actgggcgtc tac
//
LOCUS       DBNGZD010000078         2119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1678785, whole genome shotgun sequence.
ACCESSION   DBNGZD010000078 DBNGZD010000000
VERSION     DBNGZD010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1678785"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="AC16GM_02120"
     CDS             complement(<1..693)
                     /locus_tag="AC16GM_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2922501.1"
                     /translation="MKAFKRMGYLTLGFLFFGAPFQVAAGAQLSMTLEDCLRLAQEKN
                     YNLRSAAQQVELTQAKKEEAQAGFFPTLTLTGNYTYLGREPRAQIPVIPPLSPVARPI
                     TIGFHDNWDFKMGLSYTVFSWGRVGNSVAAADYAWQAAQKDAERNWHSVRLDVTRTFW
                     SVLLAQELVRVRSEAQKSLKRHQEQVAARLTAGQASRFDLLRAEVEQANALPPLLAAQ
                     HQVESGQDLLKNL"
     gene            904..1092
                     /locus_tag="AC16GM_02125"
     CDS             904..1092
                     /locus_tag="AC16GM_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR2922502.1"
                     /translation="MRKRFLFAPAFVLTLGLYPAAFSQVQVGQQAPDFTLTDVTGVPR
                     TLSQYRGQVVFLNFFGWG"
     gene            1108..1620
                     /locus_tag="AC16GM_02130"
     CDS             1108..1620
                     /locus_tag="AC16GM_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2922503.1"
                     /translation="MASSIEQNIWQVYQNQGVQVLGIDVWNGTTGQVLSYAATGNVTY
                     PLLRNGALVGRTYQGPNSWDGRDTYYIVDSTGRVNYVARYGVGAGYREAEILLHLNAL
                     LGLIGPGDVTGDIVVDITDIVALIQNVVFGTPLLVPQVADVTGDSVVDISDVVCLIQH
                     VVFATPLPCL"
     gene            1622..>2119
                     /locus_tag="AC16GM_02135"
     CDS             1622..>2119
                     /locus_tag="AC16GM_02135"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR2922504.1"
                     /translation="MRFSWRRLSLLIALFLSGTLGLFAGVPAPQFSLPDLDGKTVTLD
                     QLIGKGPLVIDFWATWCTPCTKALPKYQELYRKYRDRGLTIVAVNEDGPRSRAKVRSF
                     VSSLGLTFPVLLDTDGEVMRRFRVQGLPALLLLSAEGDILHNKQGYRPASEKELEALI
                     IPLLPE"
BASE COUNT          412 a          665 c          568 g          474 t
ORIGIN      
        1 cagatttttc aagaggtcct gacccgattc cacctgatgc tgcgccgcca gaaggggcgg
       61 aagggcattg gcctgttcga cttcggcccg caaaagatcg aagcgcgacg cctgccccgc
      121 cgtcaaccgc gccgccacct gctcctgatg ccgtttgaga cttttttgcg cttcgctgcg
      181 gacccgcact aattcctgcg ccagaagcac cgaccagaat gtccgggtta cgtccaggcg
      241 aacgctgtgc cagttacgct ccgcatcctt ttgggcggct tgccaggcgt agtctgccgc
      301 cgcaaccgag tttccgaccc tcccccagga gaaaacggtg taactcagcc ccattttgaa
      361 atcccagttg tcgtggaacc caatggtgat gggccgcgcc accggagaca ggggcgggat
      421 taccggaatc tgcgctctcg gctcccgccc caagtaagtg tagttgccgg tgagggtcaa
      481 agtcgggaaa aacccggcct gggcctcctc ttttttcgcc tgcgtcagtt caacctgctg
      541 tgcggcgctg cgcaaattgt aattcttttc ttgcgccaac cgcaggcagt cttccagggt
      601 cattgagagt tgggctcccg ctgctacctg gaaaggtgcg ccaaaaaaca aaaaacccaa
      661 cgtcaggtac cccattcgct tgaacgcttt catccgtcct cctttagtcg cttccgccgg
      721 agaataatac aggatgagcg ccgcagatgt acaactgcaa aatggaggga gaacgagtga
      781 acggatttca aatccgcgtc aggtccaaag aaaatgacgg aaattgtatg cctattgcga
      841 acttttcttg tccggaatat gtttagatta gtacagggct ttcgcccgaa ccaaggagca
      901 ggtatgcgca aacgctttct cttcgccccg gccttcgttc taacgctcgg cctttatccg
      961 gcggcctttt cgcaggtcca ggtcggacag caggcacccg actttaccct gaccgacgtt
     1021 accggtgtcc cccgcacctt aagccagtac cggggccaag tggtctttct gaactttttc
     1081 ggctgggggt gaccgtcttg cttggcggtg gcatcgtcaa tcgaacagaa catttggcag
     1141 gtatatcaga atcaaggcgt tcaggtcttg ggcattgacg tctggaacgg caccacgggc
     1201 caggtcctga gctacgccgc gaccggcaac gtcacctacc ctctgctccg caacggagcc
     1261 ttagtggggc gaacctacca agggccgaat tcctgggacg gccgcgacac ctactacatc
     1321 gttgactcca ccggtcgcgt caattacgtg gcccgctacg gcgttggcgc cggctaccgg
     1381 gaagcggaaa tcctcttgca cttgaacgcg cttttgggct tgatcggccc cggtgacgtc
     1441 acaggtgaca tcgtcgtgga catcaccgac attgtcgcgc tcattcagaa cgtcgtcttc
     1501 ggaactcctc ttttagtccc ccaggtggcc gatgtaactg gagacagtgt cgtcgacatt
     1561 tcggacgtcg tctgcctgat ccagcatgtc gtcttcgcca cccccctccc ctgtctgtaa
     1621 gatgcgtttt tcttggcgac ggctttctct ccttattgct ttgttcctca gcggaacgct
     1681 cggtcttttt gccggcgttc ccgccccgca attttcactc cccgacttgg acggcaaaac
     1741 cgtcacgctg gaccagctga tcgggaaagg tccccttgtc atcgacttct gggccacctg
     1801 gtgcaccccc tgcaccaaag cgctgccgaa gtaccaggaa ctctaccgga agtaccgcga
     1861 ccgcggcttg accattgtgg cggttaacga agatggcccc cgcagccggg ccaaagtccg
     1921 ctcctttgta tcgtcgttag gtctaacctt ccccgtcctg ctcgacaccg acggcgaggt
     1981 aatgcgccgc ttccgcgtcc agggattgcc cgcgctcctt ttgctctccg cagagggaga
     2041 cattctccac aataaacagg gctaccgccc ggcaagcgag aaagagttag aagcgttgat
     2101 catccccctt ttgcctgag
//
LOCUS       DBNGZD010000079         7734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1470291, whole genome shotgun sequence.
ACCESSION   DBNGZD010000079 DBNGZD010000000
VERSION     DBNGZD010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1470291"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /gene="carA"
                     /locus_tag="AC16GM_02140"
     CDS             <1..909
                     /gene="carA"
                     /locus_tag="AC16GM_02140"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR2922505.1"
                     /translation="KEYCAKPSNWRAEFTLDEFLKKWGIPGIAGVDTRALTRHLRDRG
                     AKMGIISADGTSEKVLVEKARKLPHLTGRDLAREVTTSQNYPWTEPSEWLPQRREVRF
                     NVVAYDYGIKQNILRKLVSYGCRVTVVPAVTPAEEVVALKPDGIFLSNGPGDPEAVTY
                     AVHAVERLVGLKPIFGICLGHQILGLAMGGRTFKLKFGHHGANHPVKNLLTGKVEITA
                     QNHGFAVDPASLGGTGLTVTHVNLNDGTVEGMKHGEYPVFSVQYHPEASPGPHDADYL
                     FEEFVQSMEQARKPERGTRYALTNAS"
     gene            complement(893..1366)
                     /locus_tag="AC16GM_02145"
     CDS             complement(893..1366)
                     /locus_tag="AC16GM_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922506.1"
                     /translation="MSVPQILGLRDLEKVNRGVLRERLKASRKRLSRLQKQKEQVESN
                     LATVTNYVQYLESLRSLTTTIADQKPKEGKHRLVVRRALRAAPRQTMGVVAYEVIRQA
                     GRPLHLKEIVERLQAQGKLRNAKRPIVAVRNTLTRLKKQFRKTKPATFAIIKTRS"
     gene            1482..4778
                     /gene="carB"
                     /locus_tag="AC16GM_02150"
     CDS             1482..4778
                     /gene="carB"
                     /locus_tag="AC16GM_02150"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR2922507.1"
                     /translation="MPRRTDLKTILIIGSGPIVIGQACEFDYSGTQACRVLKQDGFRV
                     VLVNSNPATIMTDPEVAEATYVEPLVPEVVEQIIAKERPQALLPTLGGQTGLNLAVAL
                     AEAGVLDRYGVELIGAKLPSIKLCEDRLLFKEAMCKVGLDLPRSDLATSLTQAEKIAH
                     TIGFPVVIRPSFTLGGTGGGIAYNLTELRQIVSLGLELSPAHSVLIEESVDGWKEYEL
                     EVMRDSADQCVIVCSIENLDPMGIHTGDSITVAPAQTLTDKEYQRMRDAALLIMRTVG
                     VDTGGSNIQFAVEPQSGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLEE
                     IPNDITRKTPACFEPSIDYVVVKVPRFTFDKFPEADPVLTTQMKSVGEAMAFGRTFRE
                     ALQKAFRSTEAGYTGLIPCENGNLTLDQLREKLARPHFERLQHLVQAFAAGLSVDEAF
                     RLTKIDSWFLNQIKSIVELDQRLAKTGQTGDLFRPGLNGVGPELLGQAKASGFSDIHI
                     GARLGLSEERVRALCRKWGLQPVFKAVDTCAGEFEAYTPYFYSTYEEEDEIPHSTKSK
                     VIILGSGPNRIGQGIEFDYACVHAVLATKEAGYEAILVNCNPETVSTDYDVADRLYFE
                     PITREDVLNIVEKEKPYGVLIGFGGQTPLKLALPLLKAGVPILGTQPQTIDLAEDRRK
                     FSRFLEALGIPQPQAGFATSLARAFQVAQQIGYPVLVRPSYVLGGQAMQVVFSPADLE
                     AYLKQAWEVSPEHPVLIDKFLEDAIEVDVDAVADGKTCFIGGIMEHIEEAGIHSGDSS
                     CVLPPFSLSDGQIATVADYTRRIALSLDVVGLLNIQFAIKDRTVYVLEANPRASRTVP
                     FVSKATGIPLAKVATKVALGRTLRELNLSSASPIAPPHFAVKMPVFPFIKFPGADYLL
                     GPQMKSTGEVMGLDVDLGAALLKAELAVYQKLPAAGKVFVSVKNKDKRLIGPLVRQLS
                     ALGFQLVATQGTARALAASGLAVETIAKLSEGRPNVVDMIKSGQIQIVINTPEGFVSQ
                     RDTAAIRRAALERGIPLVTTLSGTQMLVNALERLKQGKIEVRSLQEYFAPREKSEFTT
                     VVREPIQTGE"
     gene            4911..5231
                     /locus_tag="AC16GM_02155"
     CDS             4911..5231
                     /locus_tag="AC16GM_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003782420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR2922508.1"
                     /translation="MLTVSENAAHQLKDLIKQEGEKIAGLRMMVIEGGCSGYSYQMEF
                     VEAADPRDRVVEHDGVKVFVDPASAEMLDEATIDFIDGLQGGFTIRNPKAKSSCGCGK
                     SFQT"
     gene            5290..6381
                     /gene="nadA"
                     /locus_tag="AC16GM_02160"
     CDS             5290..6381
                     /gene="nadA"
                     /locus_tag="AC16GM_02160"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR2922509.1"
                     /translation="MPRTFLPIPDSYKKLPQEELIARIRQHKERLGNRMVILAHHYQR
                     EEVVHLGDYLGDSYVLAKKASQANGAEHIVFCGVHFMAESADILARPGQTDYLPNPYA
                     GCPMADMAQPEDVLRAWDEVTAVIPKEKIIPLSYMNSAASLKAFCGKNGGLICTSSNA
                     RKAFEWVFARAEKLFFFPDEHLGRNTANAMGIPPEKVIVWDFKQKLGGNSTEKIQNAQ
                     VILWKGYCHVHVTFGSDQIQEMRAKYPGIKVVVHPECTPDVVALADAVGSTTQIVNYV
                     ELMPSGSTIAIGTEINLVSRLAHEHPDMQIMQLNGVRCSMCVNMFRTSLYDLLFTLDN
                     LGKINIIRVDPEIKRDALIALNRMLEVAS"
     gene            6486..7307
                     /gene="thiD"
                     /locus_tag="AC16GM_02165"
     CDS             6486..7307
                     /gene="thiD"
                     /locus_tag="AC16GM_02165"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR2922510.1"
                     /translation="MHVALTIAGSDSGGGAGIQADLKTFAAFGVFGTSAITAITAQNT
                     VAVTGVLELPAEMVAKQIDAVFTDFGVAAGKTGMLASARIIETVAERIKHYNLKKLVV
                     DPVMVSKSGHPLLEPEATATLIKKLLPLARVTTPNLPEAEVIIGKKLSSEADRKEAAR
                     KIFETTGAAVVLKGGHLSGSAVDIYYDGTGFKELFAERVETKNTHGTGCTFSAAIAAN
                     LALGLDLWEACRIAKEYITQTLKSSVTLGHGFGPVNHFPQATVPFHGVEMPARVG"
     gene            7352..7654
                     /locus_tag="AC16GM_02170"
     CDS             7352..7654
                     /locus_tag="AC16GM_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018031534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR2922511.1"
                     /translation="MVTKEVVLEALKDCYDPEIPVNIVDLGLIYDVQVQGDTVNVEMT
                     LTAPGCHMAAPISEEAKLRIEQLDGVKAANVKVVWEPPWTPERISPEAKKMLGFGE"
BASE COUNT         1701 a         2133 c         2284 g         1616 t
ORIGIN      
        1 aaggaatact gcgctaagcc gtccaactgg cgggcggagt ttacgctgga cgaatttctg
       61 aaaaagtggg gcatccccgg cattgcgggc gtcgacactc gtgcgcttac gcgccacctg
      121 cgcgatcggg gcgccaaaat gggaatcatc tccgcggatg gcacgagcga aaaagtactg
      181 gtggaaaaag cccgcaaact gccgcacctg acgggccgcg acctggcgcg cgaagttacc
      241 acttctcaaa actatccgtg gactgaaccg tcggagtggc taccgcagcg gcgggaagtg
      301 cgctttaacg ttgtggctta cgactacggg atcaagcaga acattctgcg caaattagtg
      361 tcgtacggct gccgggtgac cgtggtacct gctgtaactc cggctgagga ggtggtggcg
      421 ctcaagccgg acgggatttt cctctccaac ggtccgggcg atcccgaagc ggtaacctat
      481 gcggtccacg ccgtggagcg tttggtgggt ctgaagccga ttttcgggat ttgcctgggg
      541 caccagattc tcgggctggc aatgggcgga cggacgttca aactgaagtt cggccaccac
      601 ggcgccaatc acccggtaaa aaatcttttg accggcaaag tcgaaatcac cgctcagaat
      661 cacggctttg cggtcgatcc cgcgtcactg ggcggcaccg gacttacggt cacgcatgtc
      721 aatctgaacg acggtacggt tgaagggatg aaacacggag aatatccggt attctccgtc
      781 cagtaccacc cggaagcctc gcccggcccc cacgatgcgg attatctttt cgaggagttt
      841 gtgcagtcga tggagcaggc ccgcaaaccg gagcggggta cccgctatgc gctcacgaac
      901 gcgtcttgat aatcgcgaaa gttgccggct ttgttttccg gaactgcttt ttgagccggg
      961 tcagggtgtt ccgcaccgca acgatcggcc gcttggcgtt ccggagcttg ccttgggcct
     1021 gaagccgctc cacgatctcc ttgaggtgga gggggcggcc cgcctgccgg attacttcat
     1081 acgcgaccac acccatggtc tgccgggggg cggctcgcag ggcgcggcgc accaccagcc
     1141 gatgcttgcc ttctttcggc ttttggtcgg cgatcgtagt cgtcaaactc cgcagcgact
     1201 caaggtactg cacgtagtta gtgaccgtgg ccaggttgga ctccacctgc tctttttgct
     1261 tttggaggcg ggacaggcgc ttccggctcg ccttcaagcg ctcccgcagg acaccgcggt
     1321 taactttctc cagatcgcgc aaacccagaa tttgggggac gctcatctta attcctcctc
     1381 attaactgtc tacaccatat atatcaatca gggtgtaagg tcaagagagc tgtttctatc
     1441 cagcgcctct tctgtaggag tcgataagcc gttcagatcc gatgccccga cgcactgacc
     1501 ttaaaaccat cctcatcatc ggctccgggc cgatagtcat cggccaggcc tgcgagtttg
     1561 actactcagg cacccaggcc tgccgggtgc tgaagcagga cggtttccgg gtcgtcctgg
     1621 tcaactccaa cccggccacc atcatgaccg atcccgaagt ggctgaggct acctacgttg
     1681 agccactggt gccggaggtc gtagagcaaa ttatcgctaa agagcgtccc caagctctcc
     1741 tcccgacatt gggcggacaa accgggctca atctggcggt ggcgctggct gaagcaggag
     1801 tactcgaccg atacggcgtt gagttaatcg gtgcgaaatt accctcgatt aaactctgcg
     1861 aagatcgact gttatttaaa gaagcgatgt gcaaagtggg cctcgacctg ccccgttccg
     1921 atctggctac ttccctcact caggcggaga aaatagcaca cacgatcggt tttccagtgg
     1981 tgattcgccc cagtttcacc ctaggcggca ccggcggcgg aattgcctac aacctcaccg
     2041 aactcaggca gattgtttcg ctgggtttgg aattgagccc ggcgcattcg gttttgatcg
     2101 aagaatcggt ggacggctgg aaagagtacg aattggaggt gatgcgcgac tccgccgacc
     2161 agtgcgtcat tgtctgctcg attgaaaact tagacccgat ggggattcac accggtgatt
     2221 cgatcaccgt cgcgccggcc cagacgctca cagataaaga gtaccagagg atgcgggatg
     2281 cggcactttt gattatgcgc accgtggggg ttgataccgg cgggtcgaat attcagttcg
     2341 cggtggaacc gcaatccggg cggatggtcg tcattgaaat gaacccgcgt gtttcccggt
     2401 cgtcggcatt agcctcgaag gccaccggtt tccccatcgc caaaatcgcg gcaaagttgg
     2461 cggtcggcta cacccttgag gaaattccca acgacattac tcggaagact ccggcttgct
     2521 ttgaaccctc catcgattac gtggtggtca aggtgcctcg cttcaccttc gacaagttcc
     2581 cggaggccga ccccgtgctg accacccaga tgaagtcggt gggcgaggcg atggccttcg
     2641 gccgaacctt tcgggaggcg ctgcagaaag ccttccgctc caccgaagca ggctatacgg
     2701 ggttaatccc gtgcgagaat ggtaatctca cacttgacca gttgcgcgaa aaactggccc
     2761 ggccgcactt cgagcggttg cagcatctgg tgcaggcctt tgccgcggga ctttcggtcg
     2821 acgaagcatt ccggctgaca aagatcgatt cctggtttct caaccagatc aagtcaatag
     2881 tagagctcga tcaaaggtta gccaaaacgg ggcaaaccgg tgacctcttc cgtccaggac
     2941 taaatggggt cggacccgaa ctcttggggc aggccaaggc cagcggtttc tccgacatac
     3001 acatcggagc gcgattggga ctctccgaag aaagagtgcg cgcactgtgc cggaaatggg
     3061 gcctccagcc ggtcttcaaa gcggtcgata cctgtgccgg ggagttcgag gcctacacgc
     3121 cgtatttcta ctccacctat gaagaggaag atgagattcc ccactcgacg aaatcgaaag
     3181 tgatcattct tggttccggg ccgaaccgga tcgggcaggg aattgaattc gactacgcct
     3241 gcgtccatgc agttttggcc acgaaggaag ccgggtacga agcgattctg gtcaactgca
     3301 acccggaaac ggtttcgacc gattacgacg tggccgaccg gctctacttt gaaccgatca
     3361 cccgcgaaga tgtgctgaat atcgttgaaa aagaaaaacc gtacggcgtc ctcattggat
     3421 ttggcgggca gacaccgctc aagttagccc tgccgctcct caaggcgggg gtgccgattc
     3481 tgggcaccca gccgcagacg atcgatttgg ccgaggaccg gcgtaagttc tcgcgcttct
     3541 tagaagcgct gggtattccc cagccccagg ccggatttgc cacgtcgctg gcccgggcgt
     3601 tccaggtagc gcagcagatc ggctatccgg tcttggtgcg gccgtcatac gttctgggcg
     3661 gacaggcgat gcaggtcgtt ttcagtccgg ctgacttaga agcgtacctc aagcaggcct
     3721 gggaggtatc gcccgagcat ccggtgctca tcgacaaatt tttggaagac gccatcgagg
     3781 ttgacgtcga tgccgtcgcc gacgggaaaa cctgtttcat cggcgggatt atggagcata
     3841 ttgaggaagc gggcatccac tccggcgatt cctcctgcgt gctcccgcct ttttccctct
     3901 cggacgggca aattgcaacc gttgccgact acacccggcg gattgcgctc agtctggatg
     3961 tggtcggatt actgaacatt caattcgcta tcaaagaccg gacggtttac gtgctggaag
     4021 ccaacccgcg cgcctcgcgc accgtgcctt tcgtgagcaa agcgactgga attcctttag
     4081 cgaaagtcgc aaccaaagtc gccctgggcc gcacattacg ggagttgaac ttgtcgtccg
     4141 cttccccgat cgcgccgccg cacttcgcgg tgaaaatgcc ggtctttccc ttcatcaaat
     4201 ttcccggggc cgactactta ttgggcccgc agatgaaatc gaccggtgaa gtcatggggc
     4261 tggatgtcga tctgggtgcg gcactcctca aggccgaatt agcggtttac cagaaactgc
     4321 ccgccgcagg caaagttttc gtcagcgtca agaacaagga caaacggctg attggccctt
     4381 tggtgcgcca gctttccgca ctcggctttc agttggtcgc cacacagggg actgccagag
     4441 cactggcggc ttccggctta gcggtagaga ccattgccaa actttccgaa ggccggccca
     4501 acgtggttga tatgatcaaa tcgggtcaga ttcagatcgt catcaatacg ccggagggtt
     4561 tcgtttccca gcgcgatacg gccgccatcc gccgggcggc cctggagcgg gggattccgc
     4621 tggtcaccac gctttcggga acgcagatgc ttgtgaatgc cctggaacgg ctgaagcagg
     4681 ggaaaatcga agtgcggagt ttgcaggagt attttgcgcc gcgggaaaag tcggaattca
     4741 caaccgtggt gcgggagccg atacagacag gggagtagaa acttggcgga gaagttccgg
     4801 agtgctccgg cttgacaggg gtattgcacg gcgtatacta cgaaagagga caaaaacagc
     4861 gtattattta gagtgaagga ttccagggcg acaattttga aaggagctat atgctgaccg
     4921 tatctgagaa cgccgctcac cagttgaagg acttaatcaa gcaggagggc gagaaaatcg
     4981 ccggactgcg gatgatggtg atcgaaggcg gctgctccgg ctactcgtac cagatggagt
     5041 ttgtggaagc cgctgacccg cgcgaccggg tggtggagca cgacggggtg aaagtcttcg
     5101 tcgatccggc ttcggcggaa atgctggacg aggcgacgat cgatttcatc gacgggctcc
     5161 agggcgggtt caccatccgg aatccgaaag ccaagtcttc ctgcggctgc ggcaagtcgt
     5221 tccagaccta aaggaagcag gtcagcgagt cgatcgaccc gcctgaggcg ggcgcccgaa
     5281 aggggaagta tgccccgtac gtttttaccg atacccgact cctacaagaa actcccgcaa
     5341 gaggaactaa tcgcccgcat ccggcagcac aaggagcggc tggggaaccg gatggtaatt
     5401 ttggcccacc actaccagcg cgaggaagtg gtacacttag gcgattactt aggggattcg
     5461 tacgttctgg ccaaaaaggc ctcccaggct aatggagcgg agcacatcgt tttctgcggg
     5521 gtgcacttta tggccgagtc ggccgacatt ttggctcgtc ccggacagac cgactactta
     5581 ccaaacccct acgccggctg ccccatggcc gacatggccc agccggaaga cgtcctgcgt
     5641 gcgtgggatg aggtcaccgc agtaattccg aaagaaaaaa tcattcccct ttcctatatg
     5701 aactccgctg cgtcgcttaa ggccttctgc ggcaaaaacg gcggcctgat ttgcacctcc
     5761 tccaacgcgc gcaaggcgtt cgagtgggtt tttgcgcgag cagaaaaact ctttttcttc
     5821 ccggacgaac acttgggccg caatacggcc aacgcgatgg gcatcccgcc ggagaaggtg
     5881 atcgtctggg actttaagca gaaattaggc ggcaattcga ctgaaaaaat tcagaacgcg
     5941 caagtgattc tctggaaggg gtactgccac gttcacgtta cgttcggctc tgaccagatt
     6001 caggagatgc gggcaaagta cccgggaatt aaagtcgtgg tacacccgga atgcacgccc
     6061 gatgtcgtgg ccttagccga tgccgtcggt tccacgacgc agattgtcaa ttacgtggag
     6121 ttgatgccct ccgggagcac gatcgccatc ggcaccgaga ttaacctggt cagccggctg
     6181 gcgcatgagc acccggatat gcaaatcatg cagctgaacg gcgtgcgctg ctcaatgtgc
     6241 gtgaatatgt tccggacaag tctctatgat ttgctcttta cgttggacaa cctgggaaag
     6301 atcaacatca tccgggtcga tcccgaaatc aagcgcgacg cgttaatcgc cctcaaccgg
     6361 atgttagaag tagcgtcgta attcaagctt tcttttattt caaggaaacc cgcgcggcac
     6421 tggttcgact gcgctcacca cgagtaaagt gccgcctacg cgttgctggt agatttgaag
     6481 acaatatgca cgttgcgttg accatagccg ggtcggattc gggcggcggg gcgggaattc
     6541 aggccgacct caagactttt gcggccttcg gggtgttcgg cacgtccgcg attaccgcta
     6601 tcaccgccca gaatacggtg gccgtgaccg gggtcctcga acttccggcg gagatggtgg
     6661 ccaagcagat cgatgcagtc ttcactgact tcggtgtagc tgccggcaag accgggatgc
     6721 tcgccagcgc gcgcatcatt gaaacagttg ccgagcgaat aaagcattac aaccttaaga
     6781 aattggtcgt tgatccggtg atggtttcca agagcggcca tcccctgctg gagcctgagg
     6841 ccaccgcgac tttgataaaa aaactcctcc ccctggcccg ggtgactact cccaacctgc
     6901 ccgaagccga ggtaatcatc gggaaaaaac tttcctccga agccgaccga aaggaggcgg
     6961 cgaggaaaat ctttgagacc accggtgcgg cggtcgtctt aaaaggaggt catttatcgg
     7021 gttcagcggt cgatatatat tatgatggga ccgggtttaa ggaactcttt gccgaacggg
     7081 tcgaaaccaa aaatacgcac gggaccggct gcactttttc agccgccatt gccgccaatc
     7141 tggcgttagg acttgatctg tgggaggcct gccggatcgc caaagagtat attacccaga
     7201 ccctgaaaag ttcggtgact ctgggccacg gcttcggccc ggtcaatcat tttccccagg
     7261 cgaccgtacc attccacgga gtggagatgc cggcgcgggt gggatgaaac tttttgaccc
     7321 gaaatttgtt taaccttaaa gaggagagtt cttggtaacc aaagaggtgg ttttggaggc
     7381 cttaaaagac tgttacgatc cggaaattcc ggtcaatatc gttgacttag ggctaatcta
     7441 tgacgttcag gtacaagggg atacggttaa tgtcgagatg acgttgaccg ctccggggtg
     7501 ccatatggcg gcgcccattt ctgaggaggc gaaattacgg attgagcagt tggacggggt
     7561 aaaggcggcc aatgttaaag tggtctggga gcccccctgg acgccggaac ggatcagccc
     7621 ggaggccaaa aaaatgttag ggttcgggga gtaaatttta cttgacagca ttttggccgg
     7681 ggtgtatatt gacctttgaa aagaggataa ccccacacgc ccgaaagaga acgg
//
LOCUS       DBNGZD010000080         4428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1817745, whole genome shotgun sequence.
ACCESSION   DBNGZD010000080 DBNGZD010000000
VERSION     DBNGZD010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1817745"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="AC16GM_02175"
     CDS             complement(<1..1709)
                     /locus_tag="AC16GM_02175"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR2922512.1"
                     /translation="MAKTENTERPQPAAPPLEERTARESLPKVYDPKLVEEKWYRQWL
                     EYGLFHGNPKTKKPVYSVVIPPPNVTAALHIGHALNHTLQDVLIRWKKLQGFETEWLP
                     GIDHAGIATQVMVEKELAKEGKTRQQLGREAFLEKIRDWKNKYGDTILNQIQKVGCAC
                     DWQRTRFTLDEGLSRAVSDVFVRLYEKGLIYRGNYIINWCVRCQTAISDDETERTEIN
                     SNLWYLRYPLEDKSGHVVIATTRPETMLGDTAVAVNPDDSRYAHLVGKTAILPLLGRR
                     LPIIADAYVDPEFGTGALKVTPGHDRNDFEIGQRHKLEVVNILNLDGTLNQNAGKFAG
                     QDRLKARAAVVEALETEGLLEKIEPYNLSAVTCSRCHSIIEPLISLQWFVKMQPLAEP
                     AARAVREGQIHFHPEHWAKVYLHWMDTIHDWCISRQLWWGHRIPVWYCRDCGEVNVAR
                     TTPAVCRKCQSKNLKQDEDVLDTWFSSWLWPFSTFGWPDETPELKKFYPTKALFTAPE
                     IIFLWVARMVMAGLEFTGKIPFADVYLHGTVRDAIGRAMSKSLGNGIDPLDIIKDHGA
                     DALR"
     gene            1880..2668
                     /gene="mazG"
                     /locus_tag="AC16GM_02180"
     CDS             1880..2668
                     /gene="mazG"
                     /locus_tag="AC16GM_02180"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR2922513.1"
                     /translation="MKRKKAQQPFDELVQIMARLRGPGGCPWDRKQNHKTLLPYLVEE
                     CYEVLEAIESRHYGKLKEELGDLLLQVVFHAQLASERGRFSVYESVADLNRKLISRHP
                     HVFGRTQRISARKVLHNWERLKLTDPSGNHRSVLDGIPRTLPALLFAFRTQEKVARFG
                     FEWKQISDVKDKIAEELTELKRAQRKRRKTAVEEELGDLFFALVTLARKSGIDPEAAL
                     RRTVRKFQRRFAGMERELQRQGKKLGTVPLRELEHLWGEAKAKR"
     gene            2996..>4428
                     /locus_tag="AC16GM_02185"
     CDS             2996..>4428
                     /locus_tag="AC16GM_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2922514.1"
                     /translation="MSRVLQKLLGSSQGWFAPLAGIILVTFFISLPSASGQSTLLHQK
                     DGDAASDFLGNSVAGAGDVNGDGKADFIIGASAADPGGRFQAGSAYVYSGADGFLLYQ
                     KDGSAAGDALGFGAAGAGDVNGDGSADFILGARGADPGGRSNAGSAYVYSGADGSLLY
                     QKDGANAGAYLGASVAGAGDVNGDGRDDFIVGAGQAGGSGQAYVYSGADGSLLFEKNG
                     GGTGQFSIAVAGAGDVNGDGKADFIVGSSLDDPVAESNAGSAWVYSGADGSLLYWKYG
                     AHTGDFLGFAVAGVGDVNGDGKADFIIGAYAANPGGVGNAGSAYLYSGADGSLLYQKD
                     GAAANDFLGNSVAGAGDVNGDGRADFIIGASLADPGGLFDAGSAYLYSGADGSLLFQK
                     DGAAANDQLGMSVDGAGDLSGDGRAEIIIGAWRADPGGVQDAGSAFVYGLPCTGRRGD
                     VGNSGSTDLEDIVALIQEVVFGTGLPNP"
BASE COUNT          898 a         1164 c         1419 g          947 t
ORIGIN      
        1 cgcagggcat cggcgccgtg atctttgatg atgtctaaag gatcgatgcc gttccccagc
       61 gacttggaca tggcccggcc gatggcgtcg cgtaccgtgc cgtggaggta aacgtcggca
      121 aagggaattt tgccggtgaa ctccagcccg gccatcacca tacgggccac ccagaggaag
      181 atgatttccg gcgcggtgaa aagcgctttg gtcgggtaga attttttcaa ctccggcgtt
      241 tcatccggcc agccgaaggt cgaaaaaggc cagagccagg aagagaacca ggtgtcgagg
      301 acgtcttcgt cctgcttaag attcttgctc tggcatttcc ggcagacggc cggtgtcgtc
      361 cgtgcaacat tcacctcgcc gcagtcccgg cagtaccaga ccgggatgcg gtggccccac
      421 cagagttggc gggaaatgca ccagtcgtga atggtatcca tccagtggag gtaaactttg
      481 gcccagtgct ccgggtggaa gtggatttgc ccctcgcgca ccgcccgggc ggccggctcg
      541 gccaaaggct gcatcttgac gaaccactgg agggaaatca agggttcgat aatcgagtgg
      601 cagcgggaac aggtaaccgc cgatagattg tagggttcga ttttttccaa aagcccttcg
      661 gtctctaacg cttccaccac ggcggcgcgg gctttgagac gatcctggcc ggcaaacttg
      721 ccggcgttct gattcaacgt cccgtccaaa ttgaggatat tcaccacttc caatttgtgt
      781 cgctggccga tttcaaaatc attgcggtcg tggccgggag tcaccttgag cgcgccggtg
      841 ccgaattcgg gatcgacgta ggcatcggca atgatcggca ggcggcggcc gagcaaaggc
      901 aaaatcgccg tcttacctac gagatgggcg tagcgggaat cgtccggatt caccgccacg
      961 gcggtatcac ccagcatcgt ttcgggccgg gtggtggcaa tgacgacgtg gccgctttta
     1021 tcctccagcg ggtagcggag gtaccagagg ttcgaattga tctcggtgcg ctcggtttcg
     1081 tcgtcggaaa tggcggtctg gcagcggacg caccagttga tgatgtagtt gccccggtaa
     1141 atcaaccctt tctcatagag gcggacaaaa acatcgctca ccgcccgcga tagtccctcg
     1201 tccagcgtaa aacgggtgcg ctgccagtcg caggcgcagc cgactttttg aatctgattg
     1261 agaatcgtat cgccgtactt gtttttccag tcccggattt tttccaggaa tgcttcgcga
     1321 ccgagttgct ggcgcgtttt cccctctttg gccagttctt tttccaccat cacctgggtg
     1381 gcgatgccgg cgtggtcgat cccggggagc cactcggtct caaacccctg cagtttcttc
     1441 cagcggatga gcacgtcctg cagggtgtgg ttcaaggcgt ggccgatgtg gagcgcggcg
     1501 gtgacgttgg gcggcgggat gacgaccgag tagaccggtt ttttcgtttt gggattcccg
     1561 tgaaaaaggc cgtactccag ccactgccgg taccacttct cctcgacgag ttttgggtca
     1621 taaactttgg gcagcgattc gcgagcggtt cgctcctcca aaggcggtgc cgccggttgc
     1681 ggtcgttcgg tattctccgt ttttgccatt acttttgatg atagggcaga taatgagctt
     1741 tgtcaagaat gcgttgtttg aatgcgggtg aactggttat actggtaacc tgtcatcgga
     1801 tttcgaacgg atttaaaacg gattagaaaa cggtgtggct ttttttgatt gagtggtgct
     1861 acatgaagga aatttcttga tgaaaaggaa gaaagcgcaa cagccatttg atgagttagt
     1921 acaaataatg gcccgcctgc gggggccggg cgggtgcccc tgggaccgga agcagaacca
     1981 taagacgctt ttgccgtatt tagtcgaaga gtgctacgag gtgttagagg ccatcgaatc
     2041 ccgccactac gggaaactca aagaggaatt gggtgacctc ctgctccagg tggtgttcca
     2101 cgcgcaattg gcgtcggagc ggggacgatt ttcggtttac gagagcgtcg ccgacctgaa
     2161 tcggaagtta atcagccgcc acccgcacgt cttcggtaga acccagcgca tttccgcccg
     2221 gaaagtcctg cacaactggg aacgactgaa gttaaccgat ccctcaggca atcaccgctc
     2281 ggttttggac ggcattcctc gaacgctccc ggcactgctc ttcgccttcc gcactcagga
     2341 gaaggtggcg cggttcggct ttgaatggaa gcagatctcc gacgttaagg acaagattgc
     2401 agaggaactg accgaactga aacgggcgca gaggaagagg agaaaaacgg cggtggagga
     2461 ggaattgggc gaccttttct ttgctctggt tacgctggcg cggaaatcgg ggattgaccc
     2521 cgaagcggcg ctgcggagga cggtgcggaa gtttcaaagg cgctttgccg gaatggagcg
     2581 ggagttacaa agacagggga agaaattggg gacggtgccg ctgagagaac tggaacatct
     2641 ctggggggaa gcaaaggcaa agagataagg tggcctgatc cgcaggagag caaccaaaac
     2701 ggggggctag gacaaggggt caggaacggc gaacctgtcg tcgcgggaag aaggacaagt
     2761 ccgcagcttt agttaaccac ttgagggaca gataattagg ggcgggactt cttgtgagtg
     2821 tggccaggat cacctaccgg gcgggaaaaa tattgataaa agggtaaaaa tgtccttgac
     2881 aagagaaaac cggttaagta ttctatcctt gcacggacac ctcgaagtcg cgtgacggat
     2941 aagcaatgaa gacttaggtc aacttcccaa ccctctttcc caagggaggt gtagtatgtc
     3001 gagggtactt caaaaacttt tggggtcttc tcaagggtgg ttcgcaccgc tcgcgggcat
     3061 cattcttgtc acttttttca tttcattacc gtccgcctcc ggccaatcca ccttgctcca
     3121 tcagaaagac ggcgacgccg caagtgactt tctgggtaac tccgtcgccg gagcggggga
     3181 tgtgaacggg gatggaaagg ccgatttcat tatcggcgct tcggctgccg acccgggcgg
     3241 acgtttccag gccggttcgg cttacgtcta ttccggtgcc gacggcttcc tgctctacca
     3301 gaaagacggc tccgccgcgg gtgacgcgct gggcttcggc gcggccgggg ccggggacgt
     3361 gaatggggat gggagcgctg atttcatcct cggtgcccgt ggggccgacc ccggcgggcg
     3421 tagcaacgcc ggttcggcct atgtttattc cggcgccgac ggctccctgc tctaccagaa
     3481 agacggcgca aacgccggcg cctatctggg cgcctccgtg gccggagccg gggatgtgaa
     3541 cggtgatgga cgagacgatt ttatcgtcgg agccgggcag gctggcggta gcggccaagc
     3601 gtatgtctat tccggcgccg acggctccct cctttttgag aagaacggcg gcggcacggg
     3661 ccagttcagc atcgccgtgg ccggggcggg agatgtgaac ggggatggga aagccgattt
     3721 tattgtcggc tcttccctgg acgatccggt agcggagagc aatgccggtt cggcctgggt
     3781 ctattccggc gccgacggct ccctgctcta ctggaaatac ggcgctcaca cgggtgactt
     3841 tctgggcttc gccgtggccg gagtggggga tgtgaacggg gatggaaagg ccgatttcat
     3901 tatcggcgcg tatgccgcca atccgggcgg ggtgggaaat gcgggctcgg cttacctcta
     3961 ttccggcgca gatggctccc tgctatacca gaaagatggt gccgccgcaa atgactttct
     4021 cggtaactcc gtagccggcg caggagacgt gaatggggat gggagagccg atttcatcat
     4081 cggcgcttcc ctggccgacc cgggcgggct tttcgatgcc ggttcggctt acctctattc
     4141 cggcgccgac ggctcgctcc ttttccagaa agacggtgct gccgcaaacg accaactggg
     4201 catgtccgtg gacggtgcgg gggatttaag tggcgacggc cgggccgaaa tcatcattgg
     4261 cgcttggaga gctgacccgg gaggagttca ggacgccggt tcggcttttg tctacggcct
     4321 gccctgcacc gggcggcgcg gggatgtcgg aaatagcggg tcgaccgacc ttgaagatat
     4381 tgtagctctg atccaggagg tagtgtttgg aactgggtta cctaaccc
//
LOCUS       DBNGZD010000081         7225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_636562, whole genome shotgun sequence.
ACCESSION   DBNGZD010000081 DBNGZD010000000
VERSION     DBNGZD010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_636562"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="AC16GM_02190"
     CDS             <1..888
                     /locus_tag="AC16GM_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2922515.1"
                     /translation="DLTINRGELFGLLGPNGAGKTTLIKILSTLLLPTSGEALIDGTD
                     VVAHPEQVRRKINVVSGGEHSGYGILNVRENLWMFSQFYGVPTKLARERIDRMLALLG
                     LTEFARTKLGKLSTGQRQKMNLIRGFVPDPQILFLDEPTLGLDVQISREVRQFIREWV
                     TNGQGKTVLLTTHYMAEADELCDRVAVIDRGRVLACDTPANLKKALRQEVVFKLEVSL
                     LTDHLERLGQLEPVRNFAYEHKPDQGRTILKLILHSDAGLPKITDFVTSNGSRILSIS
                     KMEPTLEDVFVAMVGRGLE"
     gene            885..1703
                     /locus_tag="AC16GM_02195"
     CDS             885..1703
                     /locus_tag="AC16GM_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2922516.1"
                     /translation="MKTDLKTNLRAIWGRAYVRIVGANREPSWIIFETLMPLLATLTY
                     VFVYKALKAPEDYTGFVVLGGAMTAFWLNVLWSMASQFYWEKQGGNLDLYLVAPISRM
                     AILLGMAIGGFFMTFSRAAAVVIGGVILFHVSFTVQSYGKLFLVFGLSLVALYGLGML
                     FSSLFLLFGREAWHIVTMFQEPVYLASGFYFPVRALGFYVALAASIVPVTLGMDAMRQ
                     LLFPTGRIHGLLPVNTEMLLLSGLSIFFLFLAQRALGYMEKLGKREGRLTLRWE"
     gene            1767..2504
                     /locus_tag="AC16GM_02200"
     CDS             1767..2504
                     /locus_tag="AC16GM_02200"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR2922517.1"
                     /translation="MNLKREHQKLLAEFRRQKDSKYAKNQAVFVPTKYQILGVRVPIM
                     RQIAGTWAKRHGDISPQGLFAFTDLLWKGRTLEELVFATLLLWRFRKFIPSLTQKHFD
                     SWRRKLDNWGSTDGLGVTVLGPWVLADTQRRIPYLKRLIGNRNLWSRRLALNGAIALI
                     RSQRDGKATKVAFEFIDRVKHERDPMITKAVSWLLREVAKVKPGETAAYLARNRATLP
                     ALALRETENKLRTGLKSGKRKHSRHPY"
     gene            2514..3362
                     /locus_tag="AC16GM_02205"
     CDS             2514..3362
                     /locus_tag="AC16GM_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2922518.1"
                     /translation="MRLKPNLMTLRTAGRLGWAIEANWADPLLFAIYSIVRPIFSTLI
                     LVFMYKVIAGGKTDTELFAFIYLGNAFFMYVTNLLSGMGAIVHDDREQYEMLKYIYVA
                     PINKYAYLIGRGLPKLFLTTLAVIITLTFGIFVFHLKIDWQAANWLLFLVAFPVGLFA
                     ALCLGLILAAVCLNTARHGFALSEGSAGVFYLAIGAVFPIDVLPHWLQKVSLFIPLTY
                     WLELLRRSLGAGGLSQTLSRYSNGHLLWILFLTALGLALTAHLVYQWMEHLARKQGKL
                     DLKFNY"
     gene            complement(3374..4120)
                     /locus_tag="AC16GM_02210"
     CDS             complement(3374..4120)
                     /locus_tag="AC16GM_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR2922519.1"
                     /translation="MGNLVSHFRRIEYRGKLFIFFVIWWAVWAINPVHPSDWRLENVL
                     TVLFIPILIATRNSFRLSNLSYTLIGIFLSLHTVGAHYTYAEVPYDRWFKTLFGTTVN
                     EIFGFQRNHFDRLVHFLFGLLMAYPIREVFVRIANVRGIWGYYLPLDLTMSLSMLYEL
                     VEWGASILFGGDLGAAYLGTQGDNWDAHKDMALATSGAVICMVVTALVNWRYQRDFAA
                     EMRDSLKVKGKTPLGEVKLREMREEGKGGR"
     gene            4247..5194
                     /locus_tag="AC16GM_02215"
     CDS             4247..5194
                     /locus_tag="AC16GM_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR2922520.1"
                     /translation="MDFLTHGLTGAALARFFYGKVRPLVAIITLVIGATMPDYDSVVR
                     FWSMTDFLEFHRGISHSLVGVTVAAIILASIMKAIFRDAGYLKLFVLALTGGLSHIFL
                     DMINSYGTQLFMPFTRYRYAWDLVMIIDPFITVSLILALALSVIFKRRRVLFCGIAIA
                     LILTYVGLRAVCHAIAVGTVEKFALPGEKVVASGAYPEILTPFEWLYVIETDSHYYTG
                     EVHILKGETRPNPAYTKLPEDEIVQVAKKAKTAEVFLRFAKYPYLNYHREGDRWVVTW
                     RDLRYDYPFGSLRKRNRPAFLTTVVVSADRRILEESFHF"
     gene            complement(5265..5837)
                     /locus_tag="AC16GM_02220"
     CDS             complement(5265..5837)
                     /locus_tag="AC16GM_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922521.1"
                     /translation="MIRTLSLFLPVFLLTNSLTSAADHKGHFAVGIEGGLLGPLSAAR
                     QGSDLSSLVEVSIEKFTGTNTVFGVSLLVSESELFLRPGIIPAIERLVVGWPQPKTSR
                     LTQVGVYLRAFNPTKSFVKLYGIIGPSIDRLKDRPGQTKAEYAWGIRGGFGLRLELFP
                     LVSLSFGPRLNFQSPSTFYYTASGGLVLFF"
     gene            complement(5906..6559)
                     /locus_tag="AC16GM_02225"
     CDS             complement(5906..6559)
                     /locus_tag="AC16GM_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR2922522.1"
                     /translation="MYVIIVGGGQIGLNLTKLLLGEGHEVLLVEKDPGRYANLADELG
                     EAVIQGDGGDLASLMDTGANRADVLAAVTGADQDNLIICQMAKSLYLIPRTIARVNNP
                     KNEEIFHTLGVDATVSSTRIIGALIEEKIDAGMAIPLLALKGGSVEIFQTQLEEDSPF
                     VGRKIAKVKLPEECIIMALLRGDQVIIPKGDTVLQEKDVLITLTSKEAKEEFRKLFL"
     gene            complement(6561..6962)
                     /locus_tag="AC16GM_02230"
     CDS             complement(6561..6962)
                     /locus_tag="AC16GM_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR2922523.1"
                     /translation="MRVVILGAGRVGAILAKRLLKDKHEVAVIDWRVESFRNLGKDFP
                     GQTVLGTGIDDEVLKKAGIEKADAFIAVSDSDNTNLMAVQVAKARFNTKTAIAWVVDP
                     QRAQSFQEYGLEIVCPTTLVADVIQKKLKGK"
     gene            complement(6968..>7225)
                     /locus_tag="AC16GM_02235"
     CDS             complement(6968..>7225)
                     /locus_tag="AC16GM_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2922524.1"
                     /translation="ETKAVEALNRAVEIVKLHDLEAIPSVERAREAAEGIIRVAKDNQ
                     VDLIVLGMRTNIHPTQAFFGRTTESLIRRAPCEVVIDKLPG"
BASE COUNT         1588 a         2078 c         1867 g         1692 t
ORIGIN      
        1 gacctgacca tcaaccgggg ggaactcttc ggcctcttgg gccccaacgg cgccgggaag
       61 accacactca taaagatttt atcgacgtta ttgctcccca cctccggtga agctttgatc
      121 gacggcaccg acgtggtggc ccacccggaa caggtgcggc ggaagatcaa cgtcgtctcc
      181 ggcggggaac actccggcta cggcattctg aacgtccgcg aaaacctctg gatgttctcc
      241 cagttctacg gcgtgccgac caagctcgcc cgcgaacgga tcgaccggat gctcgcactt
      301 ttgggactga ccgagttcgc ccgaaccaaa ctggggaagc tctccaccgg acaaaggcag
      361 aagatgaatt tgatccgcgg tttcgtcccc gacccgcaga ttctctttct ggacgaaccg
      421 acgttggggc ttgacgtgca gatctcccgc gaagtgcgcc agttcattcg cgagtgggtc
      481 accaatggtc aaggaaagac ggtgctcttg accactcact atatggccga agccgatgaa
      541 ctgtgcgacc gcgtagccgt gatcgaccgc ggcagagtcc tggcctgcga tacaccggcg
      601 aatttaaaga aagcgctccg gcaagaagtg gtgttcaaac tcgaagtgtc gcttttgacc
      661 gaccacctgg agcgcctggg ccaactggaa ccggtacgga atttcgccta tgagcataag
      721 ccggatcaag gccggacgat tttgaaatta atcctgcaca gcgacgcggg gctgccgaaa
      781 ataactgact tcgtcacgag taacggctcg cgcattctct cgatttcgaa aatggaaccg
      841 accttagaag acgttttcgt ggcgatggtc ggcagaggtt tagagtgaaa acagatctga
      901 aaaccaacct ccgcgccatc tggggccgcg cctacgtgcg gatcgtcggg gccaaccgcg
      961 aaccctcctg gattatcttt gagaccttga tgccgcttct ggccaccctc acctacgtct
     1021 tcgtttacaa ggccctcaaa gcgccggaag actacaccgg ctttgtagtt ttaggcgggg
     1081 cgatgaccgc tttctggctt aacgtcctct ggtcgatggc ttctcagttc tactgggaaa
     1141 agcagggggg gaatctggac ctctatctgg tcgcccccat ttcgcggatg gcgattcttc
     1201 tcgggatggc catcggggga ttttttatga ccttctcgcg ggcggctgcg gtggtcatcg
     1261 gcggggtcat cctgttccat gtcagtttca ccgtgcagag ctacgggaaa ctttttttgg
     1321 tcttcgggct ttcgctggtc gcactgtacg gattggggat gctcttttcg tcgttatttt
     1381 tgctctttgg ccgggaggcc tggcatatag tgactatgtt tcaggaaccg gtctacctcg
     1441 cttcagggtt ctacttcccc gtgcgggcgc tgggtttcta cgtcgctttg gcggcatcga
     1501 ttgtgccggt gaccttaggc atggatgcga tgcggcaact cttgttccct acgggacgga
     1561 tacacgggct cttgccggtg aacaccgaaa tgctccttct ctcaggttta tctatcttct
     1621 ttcttttcct tgcccagcgt gctctgggtt atatggaaaa attgggcaag cgggaggggc
     1681 ggctgacctt gcgctgggag tagctgtcaa cggattgcga acggattaaa cactcgactc
     1741 acacttcccg ttaagcacgc cagagaatga atctcaaacg agaacaccaa aaacttctgg
     1801 cagaattcag gcggcaaaaa gatagtaagt atgccaaaaa ccaagctgtt ttcgttccca
     1861 cgaaatacca aattttaggc gtccgggtgc ccataatgag acaaatagcc ggaacgtggg
     1921 ccaagaggca cggagacatt tctccgcagg gacttttcgc ctttaccgat ctcctctgga
     1981 aagggagaac gcttgaagaa ttggtctttg cgacgttgct cttatggcgc ttccggaagt
     2041 tcattccctc cttaacccaa aagcactttg actcttggcg cagaaaactg gacaactggg
     2101 gctccaccga cggattgggg gtgactgtcc tgggcccctg ggtgctggca gatacccaaa
     2161 gacgaatccc ctacctcaaa cggctcatcg gcaaccgcaa cctctggagc cggcgtctcg
     2221 ccctaaacgg agccatagcg ctaattcgaa gccagcgtga cggaaaagca accaaagtcg
     2281 ccttcgaatt catcgaccgc gtcaagcacg agcgcgaccc gatgattacc aaggcggtgt
     2341 cgtggctcct gcgcgaagtg gccaaagtaa agcccgggga aacagcggcc tatctcgccc
     2401 gcaatcgcgc caccttgcca gccctcgccc tccgcgaaac cgaaaacaaa ttgagaacgg
     2461 gattaaagag cggaaaacga aaacatagtc ggcaccctta ctaaattctc aagatgcgac
     2521 tgaaacccaa tctcatgacg ttgcgaaccg ccggccgcct cggctgggcg attgaagcca
     2581 actgggccga tcccttgctt ttcgccatct actccatcgt ccggccaatt ttctcgactt
     2641 tgattctggt tttcatgtac aaagtcattg ccgggggaaa gaccgacacc gaactctttg
     2701 ctttcatcta tctgggcaac gccttcttta tgtatgtgac gaatctctta tccggaatgg
     2761 gtgccatcgt gcacgacgac cgcgagcagt acgaaatgct caagtacatt tatgtcgcac
     2821 cgataaacaa gtacgcttac ttgattggta ggggcttgcc gaaattgttt ctgaccaccc
     2881 tcgccgtcat tatcaccctc acgttcggga tcttcgtctt ccatttgaaa attgactggc
     2941 aggcggccaa ctggctcctc tttttggtcg cctttccggt cggccttttt gcggcgcttt
     3001 gtctcggcct gattttagcc gccgtctgcc tgaacacggc ccgccacggc tttgctctct
     3061 ccgagggctc cgccggagtt ttctacctag ctatcggcgc ggtttttccg attgacgttt
     3121 tgcctcactg gctgcaaaag gtctctttat tcataccgtt gacttattgg ctggaacttt
     3181 tgcgccgctc gttgggagca gggggactga gccaaactct ttcgcggtat tcaaatggcc
     3241 acttgctttg gattttgttt ttgactgccc tgggcctcgc tctcaccgcg catttagttt
     3301 atcagtggat ggaacactta gcccgcaagc aggggaaatt agatctgaag tttaattact
     3361 gaggccgaag ccgtcacctt ccccctttgc cttcctcccg catctcccgc agtttcactt
     3421 cacccaaagg cgttttgcct ttcactttca gactgtcccg catttcagcg gcaaaatcgc
     3481 gctggtagcg ccagttgacg agcgcggtga caaccatgca aatcaccgct ccggacgtgg
     3541 ccagcgccat atccttgtgc gcgtcccagt tatccccctg tgtgccgagg taggccgcgc
     3601 ccagatcgcc gccgaagaga atcgacgcgc cccattcaac caattcatag agcatcgaaa
     3661 gcgacatcgt cagatccaga ggcaggtagt agccccagat gccccggacg ttggcgatcc
     3721 gcacgaagac ctcgcggatg ggatacgcca tgagcagccc gaagagaaaa tgcaccagcc
     3781 ggtcgaagtg gttgcgctga aaaccgaaga tttcgttgac cgtcgttcca aaaagcgttt
     3841 tgaaccagcg gtcgtacggt acctcggcgt aggtgtagtg cgcgccgaca gtgtgcaggg
     3901 agaggaaaat accaatgagc gtgtacgaca gattgctcag ccgaaaactg ttgcgcgtgg
     3961 ctatgagaat cggaatgaaa agtaccgtga ggacgttctc cagccgccag tcgctggggt
     4021 gaaccggatt gatggcccag accgcccacc agatgacgaa aaagatgaaa agtttcccgc
     4081 ggtactcaat gcgcctgaaa tgggagacta aattgcccat acgttcctct cgagtatggt
     4141 tgacgtttga ggaaagatac tactccctaa cgagggaaat ccagcgggtt acgatgatca
     4201 aaaaaattac ggttgaaatt ctccttcaac cgtattaact acacatatgg acttcctcac
     4261 tcacggcctc accggcgctg ccctggcgcg gtttttctat ggcaaagtcc ggcccctcgt
     4321 ggccatcatt actttggtca tcggcgccac gatgcccgat tacgatagtg tcgtgcgctt
     4381 ctggagtatg accgattttc tggaattcca tcgcggcata tcccactctc tcgtgggtgt
     4441 gacggtcgcc gccatcattc tcgcctcgat tatgaaagca atcttccgcg acgccgggta
     4501 tctgaaactt ttcgttttag ccctcaccgg cggcctctct cacatttttc tggatatgat
     4561 caactcttac ggcacgcagt tgtttatgcc tttcacccgc taccgttatg cttgggactt
     4621 ggtgatgatc atcgacccct tcatcaccgt aagtctcatt ctcgctctcg cgctatcggt
     4681 gattttcaaa cgccgccgtg tgctcttctg cggcattgcg attgccttaa tactaaccta
     4741 cgtcggcctg cgggcggtct gccacgcgat agcggtgggg acggtggaga agttcgcttt
     4801 acccggcgaa aaggtcgtgg cctccggcgc ttatccggaa atcttgacac cttttgagtg
     4861 gctttacgtc atcgaaaccg acagccacta ctacacgggc gaggtgcata tcctgaaagg
     4921 cgaaacccgc cccaacccgg cctatacgaa gttacctgaa gatgaaatcg tgcaagtggc
     4981 gaagaaagcg aaaaccgccg aggtcttcct gcgctttgcc aagtacccct acctgaacta
     5041 ccaccgcgag ggcgaccgct gggtggtcac ctggcgcgac ctgcgctacg actacccctt
     5101 cggctccctc cgcaagcgca accgcccggc gttcctgacg acggtagtgg tctcagcaga
     5161 ccggcggatt ttggaggaat cttttcactt ttaattgaag aacacccaac cagggtagac
     5221 cggctcgtcc ccgagccggt cggagaatta ccccattctt tcaactaaaa aaacaacacc
     5281 agaccgccgc tagcggtgta gtaaaaagtg ctcggggatt gaaagttgag ccgcggacca
     5341 aaagaaagtg aaaccaaagg aaacagctcg agccgtaatc cgaaaccgcc tcttataccc
     5401 caagcgtatt cagcctttgt ttgtcctggt ctgtctttga gacggtctat tgatggacca
     5461 ataataccgt agagcttcac aaaggatttg gttggattga aagctcgtaa ataaactccc
     5521 acctgggtca gtcgagacgt tttaggctgg ggccaaccaa ccactaaccg ctcgattgct
     5581 ggaatgatcc ctggccgcaa aaaaagctcc gattcgctta cgagtagact gactccaaat
     5641 acagtatttg tcccagtaaa tttttctatt gaaacttcta ctagagaact caaatccgat
     5701 ccttgtcgtg cggcggataa gggaccaagc aatccacctt cgataccaac ggcaaaatgg
     5761 cctttgtggt cagcggcaga agtaaggctg ttcgttaaaa gaaaaacggg taaaaagagc
     5821 gaaagagtcc taatcataat acccttctct attcttgcta cgttggggag ttcacattgt
     5881 aggaggtact tttcagaaaa gaaaactaca aaaacaactt cctgaactcc tccttcgcct
     5941 ctttgctcgt caaggtaatc aaaacgtctt tttcctgcag taccgtatcg cctttgggaa
     6001 taatcacctg gtccccccgc aaaagcgcca taatgatgca ctcctccggg agtttgacct
     6061 tcgcgatttt gcgcccgacg aagggcgaat cctcctccaa ctgcgtctgg aaaatctcca
     6121 ccgacccgcc cttgagcgcc aaaagcggta tcgccatccc cgcgtcaatt ttttcttcaa
     6181 tcagagcgcc gatgatgcgg gtggaagaaa ccgtggcatc gacgcctaag gtgtggaaaa
     6241 tctcttcgtt tttcggatta ttcacccggg caatcgtgcg cgggataaga tagagactct
     6301 tggccatctg gcagatgatg aggttgtcct gatccgcgcc ggtgaccgcg gccagaacgt
     6361 cggcccggtt ggccccggtg tccatcaagg aagccagatc gccgccgtcg ccctgaatca
     6421 ccgcctcccc caattcgtcc gctaaattgg cgtagcgccc cgggtctttt tcgaccaaaa
     6481 gcacctcgtg cccttcgccc aagagcagtt tggtcaaatt gagcccgatc tgcccgccgc
     6541 ccactatgat gacgtacatt tcatttccct ttgagtttct tttggatgac gtccgccact
     6601 aaggtcgtgg gacagacgat ctccagcccg tactcctgaa acgactgcgc ccgctgcgga
     6661 tcgaccaccc aggcaatggc ggttttggtg ttgaagcgtg ccttggctac ctgaactgcc
     6721 atgagattgg tgttgtcgct gtcggaaacg gcaatgaagg cgtcggcctt ctcgatgccg
     6781 gcttttttga gaacctcgtc gtcgattccg gtgcccagaa ccgtctgtcc gggaaaatct
     6841 ttgcctaaat tgcggaacga ttccacccgc cagtcgatga cggcgacttc gtgcttgtct
     6901 ttgagcaggc gcttggccag aatcgcgccc acccgccccg cccctaagat gaccactcgc
     6961 attcgtatta ccccggcagt ttgtcgatca ccacttcgca cggcgcccgg cggataagac
     7021 tttccgtcgt ccgcccgaag aacgcttgcg tcggatggat gttggtgcgc atgcccagca
     7081 caatcaaatc gacctggtta tctttggcaa cgcggatgat cccttccgcc gcctcccgcg
     7141 cccgctccac cgacgggatt gcctctaagt cgtgcaactt tacgatttcg acggcccggt
     7201 tcaaggcctc taccgctttg gtttc
//
LOCUS       DBNGZD010000082         3892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1541142, whole genome shotgun sequence.
ACCESSION   DBNGZD010000082 DBNGZD010000000
VERSION     DBNGZD010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1541142"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="AC16GM_02240"
     CDS             complement(<1..1190)
                     /locus_tag="AC16GM_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR2922525.1"
                     /translation="MARKFPLARVRNIGIMAHIDAGKTTTTERILFYTGRSYRIGEVD
                     EGAAIMDWMEQEKERGITITAAATTCVWSDHKINIIDTPGHVDFTIEVERSLRVLDGA
                     VAIFCAVGGVEPQSETVWRQADKYGVPRIAYVNKMDRVGADFDNVLEMMRKRLGANPV
                     PVQLPVGQGDLFTGIIDLIDFTFRVYDDASQGVTFDDRPIPKDLQKPAHDAREKLLEA
                     VSDYDDHLLAKFVHDEPIAHEEIAAALRRATLDGKIIPVLCGSSFKNKGVQKLLDAIV
                     DFLPSPLDMPPLVGAAMSNGNEIVRRPDDREPLTALAFKIMSDPYVGKLTFFRVYAGR
                     LASGSYVLNANTGQKERIGRLLLMHANKREEVEEVYAGDIAAAVGLRKTSTGNTLCDP
                     DHPIL"
     gene            complement(1385..1855)
                     /gene="rpsG"
                     /locus_tag="AC16GM_02245"
     CDS             complement(1385..1855)
                     /gene="rpsG"
                     /locus_tag="AC16GM_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR2922526.1"
                     /translation="MPRRRQIERRLRLPDPKYQSPLVTQFVNNLMKRGKKSTAEKIFY
                     RAVDLAGEKSGQPGLDVFQKALSNVKPVVEVKSRRVGGATYQVPVEVRPERRTALGIR
                     WLIQYGGERPGKTLAAKLAEELLAAAKNEGGAVKKKEDTHKMAEANKAFAHYRW"
     gene            complement(1895..2284)
                     /gene="rpsL"
                     /locus_tag="AC16GM_02250"
     CDS             complement(1895..2284)
                     /gene="rpsL"
                     /locus_tag="AC16GM_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR2922527.1"
                     /translation="MPTIQQLIRFGRRSVSVSSKTPALGGSPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGTL
                     DAQGVGARHQGRSKYGAKKPKEAAAKG"
     gene            complement(2357..>3892)
                     /locus_tag="AC16GM_02255"
     CDS             complement(2357..>3892)
                     /locus_tag="AC16GM_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR2922528.1"
                     /translation="TETAAQQIEESGLEAIKIRSVLTCESRRGVCAKCYGRNLATGRM
                     VDLGEAVGVIAAQSIGEPGTQLTLRTFHIGGTAARIAEQSKAAAKTDGRVVFRNLRSV
                     ARAAGETVAISRDGEINLVDGEGRLRARYNVPYAAVLHVEPESQVKKEQVLFEWDPYT
                     STIVAEQSGTVGFRDLVEGESYREELDETTGLRQRVVIEQREKALNPQILLTGERERK
                     LGTYNIPTGAHLQVHDGQKIHSGDLLVKIPREIAKTRDITGGLPRVAELFEARKPRDP
                     AIVSEIDGYVEFGKLIRGATQLFARGEDEVKEYQIPHGKHLRVHNGDRVKAGDRLCEG
                     PVDPHDILRIKGVNAVQEYLVNEIQEVYRLQGVKINDKHIEVIVRQMLQKVRIEEIGD
                     TNFLEGEQVDKFRFREENERAIAEGGEPATFQPQLLGITKAALSTDSFISAASFQETT
                     KVLTEAATNGRIDTLEGLKENVIIGRLIPAGTGLERYRSVQLLEEAEAHPAPLAEMVE
                     ERT"
BASE COUNT          630 a         1251 c         1148 g          863 t
ORIGIN      
        1 agaatcgggt ggtcgggatc gcagagggta ttgccggtgg aagttttgcg cagaccgacg
       61 gcggcggcga tatcgccggc gtacacttct tcgacttctt cccttttgtt ggcgtgcatc
      121 aaaagcagcc gtccgatccg ttctttctgg ccggtattgg cattgagcac gtaggagccg
      181 gaggccaacc gtccggcgta gacccggaag aaggtcagct tccccacgta ggggtcggac
      241 atgatcttga acgccagagc ggtcaaaggc tcccgatcgt cggggcggcg cacgatctcg
      301 ttcccgttcg acatggccgc accgaccaga ggcggcatat ccagaggcga ggggaggaag
      361 tcgacaatgg cgtctaagag tttctgcacg cccttgtttt tgaacgagga gccgcagagg
      421 accggaatga ttttgccgtc gagggtggcc cgccgcaaag ccgcagcgat ttcctcgtgg
      481 gcaatcggct cgtcgtggac gaacttggcc aggaggtgat cgtcgtagtc ggagaccgct
      541 tccaaaagct tttcgcgcgc gtcatgggcc ggcttttgca aatccttggg aatcggccga
      601 tcgtcaaagg tgaccccctg gctcgcgtcg tcgtagaccc ggaaggtgaa gtcgattaag
      661 tcgatgatgc cggtgaagag gtcgccctgg ccgacgggaa gttgaaccgg caccgggttg
      721 gcccccagcc gtttgcgcat catctctaag acgttgtcga aatcggcccc cacccggtcc
      781 atcttattga cgtaggcgat ccgcggcacg ccgtacttgt cggcctggcg ccagaccgtt
      841 tccgactgcg gctccacgcc gcccaccgca cagaagatgg ccaccgcgcc gtccaaaacg
      901 cgcaaagacc tctccacttc gatggtgaaa tcgacgtggc ccggggtgtc gatgatgttg
      961 attttgtggt cgctccagac gcaggtggtg gccgccgcgg tgatggtgat gccgcgttcc
     1021 ttttcctgct ccatccagtc catgatggcg gcaccttcat ccacttcgcc gatccggtac
     1081 gagcggccgg tatagaaaag aatcctttcg gtcgtcgtcg tcttcccggc gtcgatgtgg
     1141 gccatgatgc cgatgttgcg tacccgtgct aacggaaatt tgcgtgccat aaaaaaatac
     1201 tccactcccg cctgtgaaaa cccctctccc gtacgggcca agtggaggat ttgtctcact
     1261 ccttctgggt tttcaaaaac tcgttacgtg cttatcacct ttacccgtac gggcacggtg
     1321 ctggcgacct cctgtatttt ttctgcccta cgacctacga ccgctcaccc atcacctttt
     1381 tcggctacca ccggtagtgc gcgaaggcct tgttggcctc ggccatcttg tgcgtatctt
     1441 ctttcttttt gacggcgccg ccttcgtttt tcgccgccgc caacagctct tcggccagct
     1501 tcgccgccaa agtcttgccg gggcgctccc ccccgtactg gatgagccag cgaatgccca
     1561 gggcggtgcg ccgttccggc cgcacttcca ccggcacctg gtaggtggcc ccgccgaccc
     1621 ggcgcgactt cacctccacc accggcttga cgttggagag tgctttctgg aaaacgtcta
     1681 aacccggttg gcctgatttt tcgcccgcca ggtcgacagc ccggtagaaa attttttccg
     1741 cggtgctctt cttgccgcgt ttcatcaagt tgttgacgaa ctgcgtcacc aaaggactct
     1801 ggtacttcgg gtccggcagc cgcaggcgcc gttcgatctg tcgtctcctc ggcatctcgt
     1861 tcctcagtcc tcagggctct ggttcatttt cgattcatcc cttcgccgcg gcttccttgg
     1921 gctttttggc accgtacttg gagcggccct ggtgccgcgc acccacgccc tgggcatcca
     1981 gggtgccgcg gatgatgtgg tagcgcactc ctggcagatc tttgacccgg ccgccccgga
     2041 tgagcacgat ggaatgttcc tggaggttgt ggccctctcc gggaatgtac gcagtcacct
     2101 ccatcccgtt ggtgagccgg acccgcgcca ctttgcgcaa ggcggagttc ggcttcttgg
     2161 gggtggtggt atagacccgc gtgcagaccc cgcgcttctg gggcgatccg cccagggccg
     2221 gtgtttttga actgacgctc acgctccggc gtccgaagcg tatcagctgc tgtatcgtcg
     2281 gcactcaatt cctcctcagt agcaaacgcc tacgatcggc gcaaacctac ctatggtaac
     2341 gtctccggcg ccagtgtcaa gtacgctcct caaccatctc cgccaacggc gcgggatgcg
     2401 cctctgcttc ctctaagagc tggacgctgc ggtagcgctc caggccggta ccggcgggaa
     2461 tcaggcggcc gatgatgacg ttctccttca gtccttccaa cgtgtcgatc ctcccgttgg
     2521 ttgcggcttc ggtcagaact ttcgtcgttt cctggaacga ggcggccgag ataaagctgt
     2581 cggtggagag cgccgccttg gtgatgccca agagctgggg ctgaaaggtg gccggttcgc
     2641 cgccctcggc aatcgcccgc tcgttttcct cccggaagcg gaacttatcc acctgctccc
     2701 cttccagaaa gttggtgtcg ccgatctcct cgatccgcac tttctggagc atctggcgca
     2761 cgatcacttc gatgtgcttg tcgttgattt tcacgccctg cagccggtag acttcctgga
     2821 tttcgttgac gagatactcc tgtaccgcat ttaccccttt gatccgcagg atgtcgtggg
     2881 gatccaccgg gccttcacag agacggtcac cggccttgac tcggtcgccg ttgtgcaccc
     2941 ggaggtgctt gccgtgcggg atctggtact ccttgacctc gtcttccccg cgcgcgaaga
     3001 gctgcgtggc cccgcggata agcttgccga actccacgta gccgtcgatt tccgaaacga
     3061 tggccgggtc gcgcggcttg cgggcttcga agagctccgc cacccggggc agaccgccgg
     3121 tgatgtcgcg cgtcttcgcg atctcccgcg ggattttgac caggaggtcg cctgagtgaa
     3181 ttttctgccc gtcgtgcacc tgcaggtggg caccggtcgg aatgttgtag gtgcccagtt
     3241 tccgctcccg ctcgccggtc aaaagaatct gggggttgag ggccttctcg cgctgctcga
     3301 tcaccaccct ttgcctcaaa cccgtggtct catctaactc ttcgcggtac gactcacctt
     3361 cgactaaatc gcggaatccc accgtgccgg actgctcggc cacgatggtg gaggtatagg
     3421 ggtcccactc aaagagcacc tgctctttct tcacctggct ttccggctcc acgtggagca
     3481 ccgcggcgta ggggacgttg tagcgcgcgc gcaggcggcc ctcgccatcg actaagttaa
     3541 tttccccatc ccgggaaatg gccaccgttt ctccggcggc gcgggccacc gaacggagat
     3601 tgcggaagac cacccgcccg tcagttttcg ccgccgcttt cgactgttcg gcgatgcggg
     3661 ccgcggtgcc gccgatgtgg aaggtccgca aagtcaattg cgtgcccggt tcgccgatcg
     3721 actgggcggc gatgacaccc accgcctcgc ctaagtccac catccggccg gtggccaggt
     3781 tccggccgta gcacttcgcg cacacgcccc ggcgggattc gcaggtcaag accgaccgaa
     3841 tcttgatcgc ctccaggccg ctctcctcga tctgctgggc cgccgtttcg gt
//
LOCUS       DBNGZD010000083         4838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_429966, whole genome shotgun sequence.
ACCESSION   DBNGZD010000083 DBNGZD010000000
VERSION     DBNGZD010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_429966"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="AC16GM_02260"
     CDS             complement(<1..1003)
                     /locus_tag="AC16GM_02260"
                     /inference="COORDINATES: protein motif:HMM:NF021445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HIR2922529.1"
                     /translation="MKKRSLFVWTVALVTLASGVVNLVSVTSPALPERRAILREVFPL
                     EFLHLSRFLTLIIGFALVISAFNVYKRKKRAFQGVVLLTVLSVVFHLTKGLDYEEALL
                     ALVFLGLLAAARKRFTVKSSLPDLRSGVIRLGIAALVAFSYSVAGFWLLDKKEFGIDF
                     TLADSIKRTLLFFSLVGDPAIVPQTRHARWFVDSLYVMTATAVGYSLYALFRPALYKF
                     RTLPLERARVAEILSRHGRSALDYFKTWPDKSYFFSPSQKTFLAYRVAGNFALVLSDP
                     VGPEEEIEETVHDFMELCDENDWGLAFHQTLPDLLPIYRRLGFRKLKIGDDAIIDLTG
                     "
     gene            complement(1398..1727)
                     /locus_tag="AC16GM_02265"
     CDS             complement(1398..1727)
                     /locus_tag="AC16GM_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922530.1"
                     /translation="MKKFALILLAAAVAFMFSAPAFAQGGQATTQQAEKPAAKKEMTK
                     DTAAAHQAQTQHPAGTAEKSKPAAHPQAASHKGKARKHHTAMKAEPMKAKPDTEKVKQ
                     QSTGTPH"
     gene            complement(2167..3462)
                     /locus_tag="AC16GM_02270"
     CDS             complement(2167..3462)
                     /locus_tag="AC16GM_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR2922531.1"
                     /translation="MKLHNKIFLGLLLGALTGVGSHFLFYPDSRLEWVVTNLAYPVGQ
                     IFFRLIFMVVLPLIFSALVLGVSELGELKRLGRVGARTFGYTAIVSTISVLIGVGLVN
                     IVKPGAGLEPETRERLIASISGPGMEKEKAQVMEKAKEAQSVPETIVNLIPRNPLGAA
                     VNAFQGEILAFMVFAVLFGIALALIKSERTKPLVGFLEGVYDVVMKLIEIAMKLAPYG
                     VAALIFTATARFGLEIIKSLSQYVLVVVAGLAIHQFVVYSILVKSLARLSPKLFFSRI
                     GEVMITAFSTSSSNATLPTTMRVSEEKLGIPRKVGGFVLTLGSSLNQNGTALYEGVTV
                     LFIAQLFGVHLSIGAQLTVVLMSILAGIGTAGVPGASLPLIVIVLQSVGVPGEGIVVI
                     LGVDRILDMCRTVVNVTGDIAAAAYVARKENLLKVPERV"
     gene            complement(3449..4612)
                     /locus_tag="AC16GM_02275"
     CDS             complement(3449..4612)
                     /locus_tag="AC16GM_02275"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR2922532.1"
                     /translation="MKAVTARDERKTLDDAKKELDEAKRKFEELKKEREEEREAIEII
                     AAEKSKGFPWLARAYADYFHLQGLQEADHLEHKPHPAQVSADRVREIAGERRTIEEKL
                     RIAQWIIIYYQKLFPFLEDVIGNLDDEILQKILSRNIEKPIKEVAEVGIDPVRIYLSY
                     LPEEEYQKLSLIERNQLALDRYWKRKKSDWEIGRDYERYIGYLYEKDGYSVSYQGIIK
                     GYEDLGRDLICTKDNETEIVQCKYWSQYKTIHEKHINQLLGTTVEYWIQSQNVKNLQI
                     ELLPEFLKHCGITAVFITSTKLSERAKLFGETLGVKIRENFPIQQYPSIKCNVSRKTG
                     EKIYHLPFDQQYNRTLVEEERNECYVETVEKAERLGFRRAWRWRGPEQENETP"
BASE COUNT         1117 a         1308 c         1265 g         1148 t
ORIGIN      
        1 agccggtcag gtcgataatg gcatcgtcgc cgattttgag ttttcggaag cccagccgcc
       61 ggtagatggg cagaagatcg ggcagggttt gatgaaaggc aaggccccag tcgttctcgt
      121 cgcacagttc cataaaatcg tgaacagtct cctcgatctc ttcttccggg cccaccggat
      181 cgctcagcac cagagcaaag ttgcccgcca cccggtaggc cagaaaggtc ttttgcgacg
      241 gggagaaaaa gtacgatttg tccggccagg tcttgaaata gtcgagcgcg gagcggccgt
      301 gccgggagag gatttccgcc acccgggcgc gttcgagggg cagggtccgg aatttataca
      361 gggcgggccg gaacagagcg tagagcgagt agccgaccgc ggtggcggtc atgacgtaga
      421 gggagtcgac gaaccagcgc gcgtggcgcg tctgcgggac aatcgccgga tcgcctacta
      481 aggaaaagaa aagcagcgtg cgcttgatcg aatcggctaa ggtgaagtcg atgccgaact
      541 cctttttgtc caggagccag aacccggcca cgctgtaaga aaaagccacg agagcggcga
      601 tccccagacg gatcactccc gagcgcaggt cgggaaggct gcttttgacc gtgaatcgtt
      661 tgcgcgcggc cgcaagcaga ccgagaaaaa cgagcgccag aagcgcctct tcgtagtcca
      721 gccctttggt caggtggaaa actactgaca ggaccgtcag gagcaccacg ccctggaacg
      781 cacgcttctt ccgtttgtag acgttgaaag cggaaatgac caaagcaaaa ccgatgatga
      841 gcgtgaggaa gcgggagagg tggaggaatt cgagcggaaa gacctcgcgc aggatcgccc
      901 ggcgctccgg caaggctgga ctggtgaccg agaccaagtt caccaccccg ctggccaggg
      961 tgaccagcgc caccgtccag acgaaaagag agcgtttttt catagaggac gaccggtcgc
     1021 ccttttaaac tactacagtg ctacgatttt ataaagggcg ccgctaaaag gagatggatg
     1081 ccgtcgggct tagctcgggg agcggccctg catcgagggg tcgagagaaa ttttcgcttt
     1141 cttcgtagtg gaggaaaaaa cttttctcgc cgctcaggag agcggcgcta cagttgaatt
     1201 agactctcat tttccctccc ctttgagggg agggtgtccg tacggacggg aggggtcggg
     1261 ttacacccgc tccggcacgg agagccaagc cttttcattg ccgcgtacgc cgccggtctg
     1321 atagcgccgg aggccgtccc gttcagaatg gacggcctcc cacattgtta ctgcttctgc
     1381 gcctcggctt gcctcatcta atgaggcgtg ccagttgact gttgcttcac cttttcagtg
     1441 tccggcttgg ccttcatcgg ttccgccttc atggccgtgt gatgcttccg ggctttgccc
     1501 ttgtggcttg cggcctgcgg gtgcgccgca ggtttgctct tttccgctgt gccagccggg
     1561 tgctgcgttt gcgcctggtg cgccgcggcg gtgtccttgg tcatttcctt cttggcggcg
     1621 ggcttttcgg cctgctgagt cgtggcctgg cccccctggg caaacgccgg ggccgagaac
     1681 ataaacgcca cggcggccgc caggaggatg agcgcgaatt tcttcacttt ctcacctcct
     1741 ctttcggttg cgagtgaacg agttacggat actaagtcgg cagttccgac tggtttcctt
     1801 atcgacaaga ttgacctccc tccttgagag tctccagatg ccatttgagc gccattactt
     1861 caatcaagtt tagatacgta cctgcggacg ttgtgtgaga attatttccc tctccttgag
     1921 gagagggtgg ccgaaggccg ggtgaggtca agtttgaaaa gtttgacccc tcctggcttt
     1981 cagccatcct cccctctaag gggagggaga atcgaggctt aagttacaga agcaaccagc
     2041 gctacgatta tttttgagag atttgtccct cctctttgag gagagggcag ggtgaggtca
     2101 agcttaagaa tcgtgacccc tcccggtctg cgaccaccct cccctaaaag gagagggaaa
     2161 gacgattcac acccgctccg gcactttaag caaattctcc ttccgcgcca catacgccgc
     2221 cgcagcgatg tcccccgtca cgttgacgac cgtccggcac atatcaagga tgcggtcgac
     2281 gcccaaaatc accacaatcc cctccccggg cacgcccacg ctctgcagga cgatcacaat
     2341 caaaggcaac gacgcccccg gcacgccggc ggtgcctatg ccggccaaga tcgacatcaa
     2401 gaccaccgtc aactgcgcgc cgatgctcaa atgcacgccg aataactgag cgatgaagag
     2461 caccgtcacc ccttcgtaga gcgcggtgcc gttctggttg agcgaggagc cgagggtcag
     2521 gacgaacccg cccactttgc gcgggatgcc caatttctcc tcggagacgc gcatggtggt
     2581 cggcagggtg gcgttggagg aagaagtgga gaaggcggtg atcatcacct cgccgatgcg
     2641 ggagaagaaa agtttgggcg aaagccgcgc cagcgatttg accaaaatcg aatagaccac
     2701 gaactggtgg atcgccagcc cggcgacgac gactaaaacg tactgcgaga gcgatttgat
     2761 gatttccagc ccgaagcggg cggtggcggt gaagatgagc gcggccaccc cgtagggcgc
     2821 caatttcatg gcgatctcga tcaacttcat caccacgtcg tagacacctt caaggaagcc
     2881 gacgaggggt ttggtgcgct ccgacttgat gagcgccagc gcaattccaa acagcaccgc
     2941 aaagaccata aaggccagaa tctcgccctg aaaggcattg accgccgcgc cgagcgggtt
     3001 ccgggggatg aggttgacaa tcgtctccgg caccgactgg gcttccttcg ccttctccat
     3061 cacctgcgct ttttcttttt ccattccggg gccggaaatg ctcgctatga gccgctccct
     3121 tgtctccggc tccaatcccg cgcccggctt gacgatattg accagcccca cgccaatgag
     3181 aaccgagatg gtgctgacga tggcggtgta gccgaaggtc cgcgcgccca cccggcccaa
     3241 acgcttgagc tcacccaact ccgagacgcc caagaccaga gccgaaaaga tgagcggcag
     3301 gaccaccata aagatgagcc ggaaaaagat ttgccccacc ggataggcca aattggtcac
     3361 cacccactcc aggcgcgagt cggggtaaaa cagaaagtgg gagccgacac ccgtcagggc
     3421 tcccagaagg aggccgagga agattttgtt atggagtttc attttcttgt tctggccctc
     3481 tccatctcca cgcacgacgg aaaccaagtc tctcggcttt ctctacagtt tctacataac
     3541 actcgtttct ctcttcttct acaagggttc tattatattg ttgatcgaaa ggcaaatggt
     3601 aaattttttc tccagtcttt cgagaaacat tgcatttaat tgatggatac tgctgaatag
     3661 ggaaattttc ccttatttta acacctaaag tttctccaaa aagtttggct ctttcagaaa
     3721 gttttgtaga ggtgataaaa actgctgtga taccgcaatg tttcagaaac tctgggagca
     3781 actctatttg tagattctta acattttgag actgaatcca atactccacc gtagtaccaa
     3841 gtaattgatt tatatgtttc tcatggatcg tcttatattg agaccaatac ttacactgta
     3901 cgatttcagt ttcattgtct ttagtgcaaa tcaaatccct tccaagatct tcataaccct
     3961 taataatgcc ctgataagaa acagaataac catctttctc gtataagtag ccaatatatc
     4021 gctcataatc tctaccgatt tcccaatcgg atttcttcct tttccaatag cgatctaagg
     4081 cgagttgatt tctttcaatt aaggaaagct tctgatattc ttcttctggt aaataggata
     4141 ggtatatcct tacagggtct atgccgactt ctgcgacttc cttaattggt ttttctatgt
     4201 tgcgagagag aattttctgg aggatttcat catctaagtt tcctataaca tcttctagaa
     4261 aaggaaacaa tttttggtag taaataataa tccattgggc aatgcgcaat ttctcttcaa
     4321 tagttcttcg ctctcccgct atctcacgaa ccctatcggc tgaaacctga gcaggatgag
     4381 gcttgtgctc taaatggtcg gcctcttgaa gaccttgaag gtgaaagtaa tcggcgtatg
     4441 ctcgcgcaag ccaaggaaaa cctttacttt tttctgcggc aatgatttct attgcttctc
     4501 tttcttcctc tctctccttt tttaattcct caaattttct ctttgcttca tccaattctt
     4561 tttttgcatc gtctaaggtt ttcctttcat ctctcgcggt aacggctttc actatttgtc
     4621 tttgctgcaa gtaggcagaa atctttccaa tggcccatat taagacgatt atgcctataa
     4681 ttatcaagag ttccatattt attaaggctt taataattca tgtcaggact tagtcccgac
     4741 acagcgtcag gagcaactcc tgacgcaacc tgtaaattac attctgagtc gtctcaagac
     4801 aattcttatt agccacaaaa cgagggcgac gacgagag
//
LOCUS       DBNGZD010000084         2826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_637961, whole genome shotgun sequence.
ACCESSION   DBNGZD010000084 DBNGZD010000000
VERSION     DBNGZD010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_637961"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="AC16GM_02280"
     CDS             <1..199
                     /locus_tag="AC16GM_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HIR2922533.1"
                     /translation="RSIPVPLAWLGVLGSALLVVALPLQIAGLIKGPVTFFMWIPAGV
                     FEAVLGFWLLIKGVAKQTTAT"
     gene            272..979
                     /locus_tag="AC16GM_02285"
     CDS             272..979
                     /locus_tag="AC16GM_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR2922534.1"
                     /translation="MEMNSSSLKNTARLAGLLYLIWIITGLYAMFYVPSQINLRGDAV
                     TAAQSILSNELLFRTSIIVDLISSTLWVFMVLVLYRLFKSVDERQAKLMVALVIVQIP
                     VVFIMEAFNITSLMIFKGEILKTFELNQRQDLAMLFLRINDYGTLTLVMFWGLWLFPL
                     AILVYRSRFRPRFLGVWLIINGFALLVLSFTGLLLPQYKDMVYNIAFPAMLGEVAFML
                     WLLIKGAKPQPLAAPAS"
     gene            1030..2007
                     /locus_tag="AC16GM_02290"
     CDS             1030..2007
                     /locus_tag="AC16GM_02290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR2922535.1"
                     /translation="MKAIVHHTYGSPDVLRCEEIERPTPGGNEVLVKVRAASVNAFDW
                     RLMRGRPLFIRPMIGGLRKPKVTRPGVDVAGQVEAVGRNVTQFKPGDEVFGACRGAFA
                     EYACTTEDRLALKPANISFEDAAAVPLAALTALQGLRDKRRIQRGHKVLVDGAGGGVG
                     TFAVQIARSFGGEVTAVCSTRNVDTARSIGADQVIDYTREDFTQSGKRYDLIFAANAY
                     HSIFDYRRALGQDGIYVMVGGGWPQILQAMLLGPLLSLFGSKKMCLFGAKIDKTDLLL
                     LKDFLESGKIAPVIDRRYPLSEVAEAVRYLEEGHARGKVVITVEHCNET"
     gene            2112..>2826
                     /locus_tag="AC16GM_02295"
     CDS             2112..>2826
                     /locus_tag="AC16GM_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922536.1"
                     /translation="MRNVIWIPVLITLGLFASLAGRGAQGQEVKDGSWTVEFPVEKGE
                     LASTGRNPYFILEPGYQLVFEHGGERLTVTVLDETKTVAGVETRVVEERETKDGQPVE
                     VSRNYFAISKRTNSVFYFGEEVDIYKDGKVAGHEGGWLAGVKGAKFGLMLPGQILLRA
                     RYYQEIAPGVAMDRAEIMSMNEKVKTPAGEFDGCLKTEETTPLEPGEKEYKYYAPGIG
                     LVKDGSLPLVKYGKAGGAAK"
BASE COUNT          626 a          697 c          832 g          671 t
ORIGIN      
        1 ccgcagcatc cccgttccgc tggcctggct gggcgtactc ggctcggcac tgctcgtcgt
       61 agcccttccc ctgcagatcg ccggactcat caagggcccg gttaccttct ttatgtggat
      121 accggcgggc gtgttcgagg cggtgctcgg gttttggctg ctcatcaagg gcgtcgccaa
      181 gcaaacaact gcaacttaac ttgagagtta taccgcttgc gtttttaaat acattgtgcg
      241 tcattataaa aaaccttaag gagcaaatcg tatggaaatg aattcgagtt cactcaaaaa
      301 caccgcaaga cttgccggcc tactgtatct tatttggata ataacaggcc tctatgccat
      361 gttttatgta ccatcacaaa ttaatctacg gggtgatgct gttaccgctg cccaaagtat
      421 cctatccaat gaattattat ttcgaacgag cataattgtg gatcttatca gcagtactct
      481 ttgggtgttt atggtattgg ttctttaccg attgttcaaa tcggtagacg agcgtcaggc
      541 taaacttatg gttgcattgg tgattgtgca aattcctgtc gtttttatta tggaggcatt
      601 caatattact tcccttatga tttttaaagg cgaaatattg aaaacatttg aacttaatca
      661 gagacaagat ttggctatgc tatttcttag aattaacgac tacggaacgt tgacattagt
      721 aatgttctgg gggctttggc ttttcccctt agcgatcctt gtctacaggt cgcgctttcg
      781 tccccgcttt ctgggcgtct ggctgatcat caacggcttc gcccttctgg tcttgagctt
      841 tacgggctta ctgctaccgc aatacaagga tatggtctac aatatcgcct tcccagccat
      901 gctcggagag gttgcgttta tgttgtggct tttgatcaag ggcgcaaagc cgcagccgtt
      961 ggccgccccc gcctcttgac cggcttatcg ctcagtagag tctgccttgc aaactctgag
     1021 acgaaactca tgaaagctat tgtgcatcac acctatggtt cccccgatgt tcttcggtgt
     1081 gaagaaattg aaaggccgac cccggggggg aatgaagtcc tagtaaaagt tcgcgcggct
     1141 tccgtcaatg catttgactg gcgcctcatg agaggcagac cgttgttcat ccgcccaatg
     1201 atcggggggc tgcgcaaacc aaaggtcaca cgacccggcg ttgatgtggc cggccaggtc
     1261 gaagcggttg gcaggaacgt aacccagttc aaaccgggcg acgaggtgtt cggcgcgtgc
     1321 cgtggggcct ttgccgagta tgcgtgtacg actgaagatc gattggcgct gaagccggcc
     1381 aacatatcgt ttgaggacgc agctgccgta cccctggcgg cactcaccgc ccttcagggt
     1441 cttcgtgata agcgacgcat ccagcgaggc cacaaggttc tggttgatgg cgcgggtggt
     1501 ggggtgggca cgtttgcggt ccagattgcc agatcgttcg ggggtgaagt cactgccgtg
     1561 tgcagcacga ggaacgtgga cacggcgcga tcgattggcg cagaccaggt cattgattac
     1621 actcgggaag atttcacgca gagcgggaag cgctacgacc tgatttttgc tgcgaacgcc
     1681 tatcattcga ttttcgatta ccggcgtgcg ctcggtcagg acggaattta tgtcatggtt
     1741 ggaggtggtt ggcctcaaat tctccaagct atgttactgg gaccattact gtcactcttt
     1801 gggagcaaga agatgtgtct ctttggagcg aagatagaca agacggattt gcttcttctg
     1861 aaggattttc ttgaatccgg aaagattgca cccgtcatcg atagacgtta cccgttaagc
     1921 gaggtggcgg aagccgtccg ttatcttgaa gaaggacatg ccagaggaaa agtagtaata
     1981 actgtggaac attgtaacga aacctaacag cgccttgcac ccgtcggccg cagcgcagtt
     2041 gggaagcggc cgcggctgaa gcgccggccg ttagtcgttc gtcgggcgca ctcaaattcg
     2101 ggagaatgat catgcgcaat gtaatctgga ttccagttct cattaccctc gggcttttcg
     2161 cctccctggc gggccggggg gcgcaaggac aagaagtgaa ggacggctcg tggactgtcg
     2221 agttcccggt ggaaaagggg gagttggcgt ccaccggacg caacccctat ttcatcttag
     2281 agcccggcta ccagttggtc tttgagcacg ggggagagcg gttgaccgtt acggtcttgg
     2341 acgagaccaa aaccgtggcg ggcgtggaga cgcgggtggt ggaggagcgc gagaccaaag
     2401 acggccagcc ggtcgaggtc tcacggaact atttcgccat cagcaagcgg acgaacagcg
     2461 tgttctactt cggggaagag gtcgatatct acaaggatgg caaggtcgcc ggccacgagg
     2521 gcgggtggct ggcgggcgtg aagggcgcta aattcggcct gatgctgccg ggtcaaatcc
     2581 tgctcagggc gcggtactac caggagatcg cccccggcgt cgccatggac cgggccgaga
     2641 ttatgagcat gaatgagaag gtgaagacgc ccgccggaga attcgacggc tgcttgaaga
     2701 ccgaagagac gactcccctg gagccgggcg aaaaggagta caagtactac gccccgggga
     2761 tcggtctggt caaagatggg tcgctcccgc tcgttaaata cgggaaggcg ggaggagcag
     2821 cgaaat
//
LOCUS       DBNGZD010000085         3468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_637974, whole genome shotgun sequence.
ACCESSION   DBNGZD010000085 DBNGZD010000000
VERSION     DBNGZD010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_637974"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..2887)
                     /locus_tag="AC16GM_02300"
     CDS             complement(278..2887)
                     /locus_tag="AC16GM_02300"
                     /inference="COORDINATES: protein motif:HMM:NF049220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7451 domain-containing protein"
                     /protein_id="HIR2922537.1"
                     /translation="MRKRDLIGPVILLAAGLVCNALGAEQKPNSDASQPKNTALQVTP
                     VGKTGWAPSKQLTGPQDVCPFTMACFSGQVAFTVSGLPGGELCEYQDPAKTCPPPAAC
                     PTYPFEVKNVHILFSNANATACVLLVTPGIRRADFTNPACPVPGAMICTGPVQQIVIP
                     PNTQCMELIFPLNPGCCVDTTYFACLNFQVNCPPPGVGPCLDNAPCDPCCSYWDQTTG
                     TFIDWCTVIPSGGNLMLWTDGTLSCQNNCPRRPTGKNHYKTWRVLPNPTTILSATVSD
                     QFIQNDPVRLDSIQYLSNPTRKVTINPTRNDTFNIVDTTQHLSWYKAIGRDLLLQVRY
                     GNQFETTAVVIDSMRYLLVPTQKQPHGPPQGLDHYKAYRIKNPTAFSRQVQLQDQFDG
                     ANPELIQQLVPVYFLTPCRKNADPFFDDTTHYVAYRIAPQRTTTQQRQTQDQFGMHVL
                     TSVVSELLLVPTKKLSFGPPPPPECSDGLDNDGDGLIDFPADCGCINPSDNTEAPNPV
                     TQCNDGIDNDGDGLIDLADCGCKDVCDNSESPDAVTQCNDGIDNDGDGLIDFPADPGC
                     ANACDNDEFNPPPTTRKNHYKTWRLLPNPTQFLTAQVRDQFIQNDFLRLDSIQYLSNP
                     TRKITINPTRNDTFNIVDTTQHLSWYRARGLDTLLTVRYENQFESTTVQIDSVRYLLV
                     PTQKQPHAPPQGLDHYKAYRIRNGVPFTRPVQLQDQFDPVPEQIQTLVPVYFLTPCIK
                     NADPFFDLTTHYVAYSIFPPRTITQTRGTFDQFGPHVLTSIESDFLLVPTTKIGFCPS
                     IPGDLTGDGVKDIVDIVALINCVVFNDCPFANPPCCDLNCDGICDIVDIVCLINCVVF
                     VTQCTGCPFNWCP"
BASE COUNT          734 a          927 c         1027 g          780 t
ORIGIN      
        1 aaaacgttcc aggtgggttg gcgtgcgggt ctggccgtcc gccgtcccta acgggaaaag
       61 gggtgcgaat ccccacagga cgaacccgct taacaagaca agtccgagcc atcctaccac
      121 tgcatggatt gtcccagtag tcgagtcacg cagagaacta ttcgtcggat ggccattggt
      181 ctgagggagg ttgcctttcg gcagcgggtc tccaaaaaaa gagggcagcc ctttcggact
      241 gccctccggt gtcacttttt ggcttctccc cttcccctta cgggcaccag ttgaacggac
      301 accccgtaca ctgcgtcacg aagaccacgc agttgatgag gcagacgata tccacgatgt
      361 cgcagatgcc gtcgcagttc aaatcgcagc agggtgggtt ggcaaagggg caatcgttga
      421 agaccacgca gttgatcagc gccacaatgt ccacaatgtc cttgaccccg tcgccggtta
      481 agtcacccgg tatcgaggga cagaaaccga ttttcgtcgt cggcaccaat aggaaatcgc
      541 tctctatgct ggtcagaacg tgcggcccga actggtcgaa cgtgcctcgc gtctgggtaa
      601 tggtgcgcgg aggaaaaatg ctgtaggcga cgtagtgggt cgtcaggtcg aagaacgggt
      661 cggcattctt aatacagggg gtgaggaagt agaccggcac cagcgtctgt atctgctcgg
      721 gtacgggatc gaactggtct tgcaattgaa ccggcctcgt aaacggcacc ccgtttctaa
      781 tccggtacgc cttgtagtgg tccaagccct gcggcggggc gtgcggctgc ttctgggtgg
      841 ggacgaggag ataccgcacg gagtcgatct ggaccgtggt tgactcgaat tgattctcgt
      901 accgcaccgt cagcagggta tccaaacctc tggcccggta ccagctcagg tgctgcgtgg
      961 tatcgacgat gttaaaggta tcgttccttg tcgggttaat ggtaatcttc cgggtcgggt
     1021 tggagaggta ctggatactg tcgagccgca ggaaatcgtt ctggatgaac tgatccctca
     1081 cctgggcagt caggaattgg gtcggattgg gcagcagtct ccaggttttg tagtgattct
     1141 tccgcgtggt gggcggagga ttgaattcgt cgttgtcgca cgcattcgcg caacccgggt
     1201 cagccgggaa gtcaatcagc ccgtcgccgt cattgtcaat cccatcgttg cactgggtca
     1261 ccgcatcggg cgattcgctg ttgtcgcaca catctttgca cccgcagtcg gcgaggtcaa
     1321 ttaaaccgtc gccgtcgttg tcgattccgt cattgcactg ggtgaccgga ttgggcgctt
     1381 cggtgttgtc agacggattg atgcagccgc agtcggccgg aaagtcaatc agcccgtcgc
     1441 cgtcattatc caaaccatcg ctgcactcgg gcggtggggg cggaccaaag gacaacttct
     1501 tcgtcggcac gagcaagagc tcgctcacca cgctggtcag aacgtgcatt ccaaattggt
     1561 cttgggtttg cctctgctgg gtggtggtgc gctgaggagc aatgcggtag gccacgtagt
     1621 gggtggtgtc gtcgaagaac gggtcggcat tcttcctgca gggcgtgagg aagtagaccg
     1681 gcaccagctg ctgtattagt tcggggttgg caccgtcgaa ctggtcttgc agttgaacct
     1741 gccgcgagaa cgccgtcggg tttttaatcc ggtacgcttt gtagtggtca agcccctggg
     1801 gcggcccatg cggctgtttt tgcgtcggca ccagaaggta ccgcatcgag tcgatgacca
     1861 ccgcggtggt ctcaaactga ttcccgtacc tcacctggag caaaaggtcg cggccgatcg
     1921 ctttgtacca gctcaggtgc tgcgtggtat cgacgatgtt gaaggtatcg ttccgagtcg
     1981 ggttaatggt aaccttccgg gtcgggttcg agaggtactg gatgctgtcg agccggacgg
     2041 ggtcgttctg gatgaactgg tctgataccg tagcgctcaa gatggtcgtc gggttgggca
     2101 gaaccctcca ggtcttatag tgattcttgc cggttgggcg ccgcgggcag ttgttctggc
     2161 aggacaaagt cccatcagtc cagagcatta gattgccgcc tgagggaata acagtgcacc
     2221 agtctataaa ggtgcctgtc gtctgatccc aataggagca acaagggtca caaggggcgt
     2281 tgtcaaggca aggaccgacc ccggggggag gacagttgac ctggaaattc aaacaggcaa
     2341 aataggtggt gtccacacaa caacccggat ttagggggaa tattaattcc atacactgag
     2401 tgttgggggg tatgacaatc tgctggaccg ggccggtaca aatcatagcc cccggcaccg
     2461 gacaggccgg gttggtaaaa tcggcccggc gaatacccgg agtgaccaga agcacacagg
     2521 cagtagcatt cgcattggag aagagtatgt gtacgttctt gacctcaaaa gggtaagtag
     2581 gacaggcggc aggaggaggg cacgttttgg ccggatcctg atattcacac agctcccccc
     2641 ccggaaggcc actcacggtg aacgctacct gtccgctgaa gcaggccatg gtgaagggac
     2701 agacatcctg tggcccggtt agctgctttg agggtgccca ccctgtcttg cccactggcg
     2761 ttacctgaag agccgtgttc ttcggctggc tggcgtccga gtttggtttt tgttccgctc
     2821 ccagagcgtt acagacaaga cctgccgcca ggaggattac cggaccgatt aaatctcttt
     2881 tccgcatgtg cctctccttt ccgttttctc tttcgactct ctttcgctac cgatggttcg
     2941 gggaaaaaat aaaaaacacc tctaaacgaa ggtgctcttg gttaacctcc acttctttgc
     3001 cccaatagga cattttcgcc caccctggtg ccataactca cacgcctaca ctccaccctt
     3061 cacacaggcc gcgcttcaac tcctctttta agtcggcacc tccccttcag caattaaata
     3121 tagcaggtca tattttcttg tcaaggaaaa tcttggctca gcccaaaaaa tctttgtgaa
     3181 gaacatcaca agcccaccaa cggcgggtta agggatgccg gaaaccgtcg ataaatctta
     3241 aagaaaacca tagagttctg ttcggatatc tctttcggag gcaaataccg tggctacctg
     3301 cggacgctgt ggtaaagcgg aagttgaatc gggcgaacaa tactgttctc cttgtagtgc
     3361 ggaattgaag aaggaccctc ccgtccctgc tccggccggg gatgatccgc tgccggtttc
     3421 ttctccggta acgatctgga cgggaccgga atcggccatg cacgaccc
//
LOCUS       DBNGZD010000086         2568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_638404, whole genome shotgun sequence.
ACCESSION   DBNGZD010000086 DBNGZD010000000
VERSION     DBNGZD010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_638404"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..576
                     /locus_tag="AC16GM_02305"
     CDS             175..576
                     /locus_tag="AC16GM_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR2922538.1"
                     /translation="MYDGYNATITDHLKNPRNAGDLPGADVVATDKNDYCGDIMRLSL
                     KVVDGIIADLRFKTFGCGISIAAGSMMTVLAQGKTVSEVQQVTENQIASALGGLAPGK
                     IHGAQLAAAVLQKAVAEYLAKSANGSRGAGR"
     gene            536..1330
                     /locus_tag="AC16GM_02310"
     CDS             536..1330
                     /locus_tag="AC16GM_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2922539.1"
                     /translation="MRKAPTAAGAQAGDMNPLPLTHPSPTVETSLDLVRAVPFFRTLA
                     DADAAPLIGFFHERKFAKGDYLFWEGDAADWMYVIRSGKVRMFKSSPNGKEINLEIMA
                     PGEFCGGGAIYVDVQPASAQALTETVACGIRKTDFQEVLTRHPRLAQEVIAYLGQKLM
                     SAHDMILSLITSRVENRVASLLVRLAEHHGTKTPEGVRINIALTRQNIADVIGSTVET
                     TIRVMRRFEREKILGTREGRIMIFQLDRLQKMQFAQESGPSKKQRK"
     gene            1387..>2568
                     /locus_tag="AC16GM_02315"
     CDS             1387..>2568
                     /locus_tag="AC16GM_02315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2922540.1"
                     /translation="MTSNQISPVIPAHLKPPVRPGEFVSGLAAASENRIDVGVLFVGA
                     GPAGLGGAIRLAQLLEQAPKVKEALGEVPIAVIEKGKYPGAHLVSGAVVNPVVLRKLF
                     PDLKDGDFPFFGRVEKERVYFLTARQAFRAPFLPPTMRNHGNFVASLSRLGKWLAEKA
                     EAAGVTILSETVGMKLLVKNGAVCGVQTDDKGRDREGNPLPNFQPGSEIIAKTTVLGE
                     GTTGHLSQAAVEHFRLTRPNPQIYALGVKEIWEIPKPLDSVIHTMGWPLSSSKKAREF
                     GGSFIYPMGRNMVSIGLVVGMDYADAALSVHDLLQEFKTHPLIWNILSGGKRVGWGAK
                     AIPEGGFYSLPEPLHAPGALFVGDAAGFVNVPALKGIHYALWSGVLAAEAIFEALQNN
                     RN"
BASE COUNT          560 a          761 c          737 g          510 t
ORIGIN      
        1 tacctgaagt cgctgtaaat tgtaggataa aatccagccg aggagcatag tgcgggtgcc
       61 ctttctaaac aagggcaccc gtatctgccc acgcaggcct tcccgcccag acccgtactg
      121 cgccgagtta tatgttctca tttggccaag tgttgtatat tgctttgacg aattatgtat
      181 gacgggtaca acgccacaat caccgaccac ctgaaaaacc cccgcaatgc cggtgacctt
      241 ccgggggccg acgtggtcgc cacggacaaa aatgactact gcggggacat catgcggttg
      301 tcgctgaaag tggtggatgg aataatcgcc gacctgcgct tcaaaacctt cggctgcggc
      361 atttccattg ccgcggggtc gatgatgacc gtgctggcac aagggaaaac cgtttccgaa
      421 gtgcaacagg tgaccgaaaa ccagatcgcc tctgccctgg gcgggctggc gccggggaaa
      481 atacacggtg cgcagttggc cgcggcggtc ttgcagaaag ccgtagcgga gtatcttgcg
      541 aaaagcgcca acggcagccg gggcgcaggc cggtgatatg aacccgctcc cccttactca
      601 cccgtcgccg actgttgaaa ccagcctcga tctggtgcgc gccgttcctt tcttccgtac
      661 cctggccgac gcggacgccg caccgctcat tggattcttc cacgaacgga aattcgccaa
      721 aggcgattac ctcttttggg agggcgacgc cgccgactgg atgtacgtca ttcgttccgg
      781 caaagtgcgc atgttcaaaa gctccccgaa cggcaaggaa atcaatctcg aaattatggc
      841 tcccggagaa ttctgcggcg gcggcgccat ctacgttgat gtccagcccg cgtcggccca
      901 ggccctcaca gaaaccgtgg cctgtggcat ccggaagacc gacttccagg aggttttaac
      961 gcgccatccg cggctggccc aggaggtcat tgcctacctg gggcagaagt tgatgtcggc
     1021 ccacgacatg atcctgagtc tcatcaccag ccgcgtggag aaccgcgtgg cgagcctcct
     1081 ggtccgcctg gccgagcacc atgggaccaa aacgccggag ggcgtacgca tcaatatcgc
     1141 cttgacccgg cagaacatag ccgacgtcat cggcagtacg gtggagacca ccatccgcgt
     1201 gatgcgccgc ttcgagagag aaaaaatcct gggaacccgg gaaggccgaa tcatgatttt
     1261 ccagctcgac cggttgcaga aaatgcaatt cgcccaggag agcgggccgt caaagaagca
     1321 aagaaaataa tcccgctccg cagatcgagc gtcactgatg caccagacgc gaatgctgac
     1381 agctgaatga cctcaaacca aatttcccca gtcattccgg ctcacttgaa accgcccgtc
     1441 cgtcccggag aattcgtttc cggtttggcc gctgcgtcgg aaaatcggat cgacgtcggc
     1501 gtgctcttcg tcggcgccgg cccggccggt ttaggcgggg caatccggct ggcgcaactc
     1561 ctggaacaag cgcccaaagt caaagaggcc ttaggtgaag tccccattgc ggtgattgaa
     1621 aaagggaaat atcccggagc gcacctggtc tccggcgcgg tggtcaaccc ggtggtcttg
     1681 cgaaaactct ttcccgatct caaagacggt gattttccct tcttcggccg ggtggagaaa
     1741 gagagagtct actttcttac cgcccgtcag gcctttcgcg cgcctttcct gccgccgacg
     1801 atgcgcaatc atggtaactt tgtcgcttcc ttaagccgtc tgggcaaatg gctggccgaa
     1861 aaagccgaag cggccggtgt gacaatttta agcgaaaccg tcggcatgaa acttctcgtg
     1921 aaaaatggcg cggtctgcgg cgtccagact gacgacaaag gccgcgaccg ggaaggcaac
     1981 ccgctgccga atttccagcc cggctcggaa atcatagcga aaaccaccgt tctgggcgaa
     2041 ggcacgacgg gacacttatc gcaggcggcg gttgaacatt ttcggctgac ccggcccaac
     2101 ccgcagattt acgccttggg tgttaaggaa atctgggaaa taccgaagcc gcttgactca
     2161 gtcatacaca cgatggggtg gccactttcc agttccaaga aagcgcgcga gtttggcggc
     2221 tcattcatct acccaatggg ccggaacatg gtttcgattg gcctcgtcgt agggatggat
     2281 tatgccgatg ctgccttgtc cgttcacgac ctcttgcagg aattcaaaac tcatccgttg
     2341 atctggaata ttctctccgg cggcaagcgc gtcggctggg gagccaaagc cattcccgaa
     2401 ggtggtttct actccctacc ggagccgttg cacgcaccgg gagcgctttt cgtcggcgat
     2461 gccgccggct ttgtgaacgt tccggcactc aaggggattc actacgccct ctggtcgggt
     2521 gtcctggccg ctgaggcgat ctttgaggcg ttgcaaaaca accgcaat
//
LOCUS       DBNGZD010000087         2569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1820353, whole genome shotgun sequence.
ACCESSION   DBNGZD010000087 DBNGZD010000000
VERSION     DBNGZD010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1820353"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="AC16GM_02320"
     CDS             complement(<1..543)
                     /locus_tag="AC16GM_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2922541.1"
                     /translation="MPPNHHQNKGKSAPRLFRALRNRNYRLFFGGQSTSLIGTWLTRV
                     ATSWLVYRLTHSAFLLGVVGFAGQIPTFLLAPLAGVLVDRWDRRRLLVLTQVLSMFQS
                     LSLAFLALAGLIAVWQIILLSILQGCINAFDTPARQSFVVEMVEKREDLPNAIALNSS
                     MFNSARLIGPSLAGLLIAAVG"
     gene            complement(913..1437)
                     /locus_tag="AC16GM_02325"
     CDS             complement(913..1437)
                     /locus_tag="AC16GM_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIR2922542.1"
                     /translation="MWIFNHTLLEIALRAAIIYVVVLIGIRLAGKREVGQMTPFDLVL
                     LLLIANAVQNAMTGPDTSVTGGLVAAGTLLVANVVVSRLVWRYKKIRRLAEGTPTLLI
                     HSGKVLTENLAKEKLTTEELEQALREHGVASAGEVSQAVLEIDGAISVLRKDEMPAVA
                     RPHHRIRFVGRKSI"
     gene            complement(1540..>2569)
                     /locus_tag="AC16GM_02330"
     CDS             complement(1540..>2569)
                     /locus_tag="AC16GM_02330"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR2922543.1"
                     /translation="LTIIPRPPAKSARRFFQSVFVYLLAVAGLVWVFYDIRVEKLWRT
                     LPAIDWWWVVPAVVCDTLSYVCQGLRWQLLLRPVGTLPIGRATQAVYAGLFTNEILPM
                     RIGEFVRAYLVSRWISAEFATVIPSIVVERLLDGVWLALAIGLTAIFIPLPKDLLEAG
                     DFLGIIILAATGFFLYLIYRRRNVPADHWGLSPGWKPVRVLLSMAGRLADGLRKIGTS
                     RFLAGAAGVSLLMFLFQALAFWLVMIAYGFQQSFWVGAVVFLIVHLGTALPNAPSNVG
                     TYQFFTVVGLTLFGVEKTAATGFSVVVFLILTVPLWLIGSFALSRSGTTLSAIRSEIK
                     RLLSAVRK"
BASE COUNT          609 a          753 c          762 g          445 t
ORIGIN      
        1 cccgactgcg gcgatgagca gcccggctaa ggatggccct atcaagcgcg cgctgttgaa
       61 catggaggaa ttcagggcaa tggcgttggg caggtcctcc cgtttttcga ccatttccac
      121 cacaaacgac tgccgggccg gcgtgtcgaa cgcattgatg cacccctgca agatggagag
      181 gagaataatc tgccagacgg caatgagacc agccagggcc agaaatgcca gcgagaggga
      241 ctggaacatc gacaaaacct gcgtcagcac caaaagccgc cgccggtccc accgatcgac
      301 caaaacaccg gccagagggg cgaggagaaa agtcgggatt tgaccggcga agccgacgac
      361 gccgagtaag aaggccgaat gcgtcaggcg atagaccagc cagctagtgg cgacgcgggt
      421 caaccaagtt ccgatgagtg aggtactctg gccgccgaaa aagaggcggt aattcctgtt
      481 ccgcagcgcg cgaaaaagcc gcggcgcgct ttttccctta ttctgatgat gattcggcgg
      541 catctattta tctgcgggac tgcttgctaa cttttacggg cacccacatt tactgtttgt
      601 ttgccgttgc cgccatctgg ccaattcctt ttccgtttgc atcggccagc gcagtatctc
      661 cccgcctgac cccttccgtc ccgatacacc gggacaccct cccctacaag gggaacatct
      721 ctctaaacac acggtggcgc agattggctt gcccctgagt ccgcctgagg cggatcgaag
      781 ggttttggta atccgcgcca aagatggttt ggtgcaggtt acgcagacca acctgcacta
      841 cgtttgttat ggaagattct ccctctcctg taggagaggg tggccgtagg ccgggtgagg
      901 tcagaaagag gattagattg atttcctgcc cacaaatctt atccggtggt gcggacgagc
      961 aacggcgggc atttcgtcct tgcgcagaac gctgatggcg ccgtcgattt cgagcaccgc
     1021 ctggctcacc tcgccggcgc tggcgacgcc atgctcccgc agggcctgct ccaattcttc
     1081 agtggtcaac ttttctttcg ccaggttttc tgtgagaact tttcctgagt gaatgagcag
     1141 agtcggcgtc ccctccgcca accggcggat ttttttatag cgccagacca agcgcgacac
     1201 cacgacattg gccaccagaa gcgtccccgc tgccaccaag ccgccagtga ccgacgtatc
     1261 cggcccggtc atcgcattct gcaccgcgtt ggcaatcaga agcagtaaga ccaaatcgaa
     1321 gggcgtcatc tggccgactt cgcgcttgcc ggcgaggcgg atgccgatta agacgacgac
     1381 gtaaataatc gccgctcgca gggcaatttc aagcagggtg tgattgaaga tccacacgga
     1441 ggagattcta cgctcggttg gagacgaggt caagcggaaa gccggcgccg aaagaaaagt
     1501 tcgtgatcga gagaaaaacc gaaaatgaaa acgccggctt cacttcctga ccgcgctcaa
     1561 tagcctcttg atttcactgc ggatcgcgga caaagtcgtc ccgctgcggc tgagggcgaa
     1621 cgagccgatg agccacaggg gaaccgtcag aatcaggaac accaccacgg aaaagccggt
     1681 ggccgccgtt ttttccactc cgaagagcgt caaaccgaca acggtaaaaa attggtacgt
     1741 ccccacgttg gagggcgcat tgggcagggc ggtccccagg tggacgatca aaaagacaac
     1801 cgcccccacc cagaaggatt gctggaaacc gtacgcaatc atcaccagcc agaaggccag
     1861 ggcctggaaa aggaacatga ggagtgatac gccagcggcc ccggcgagga agcgggacgt
     1921 tccgatcttt cggagcccgt cagccaggcg tcctgccatc gacagaagca cgcgcaccgg
     1981 tttccagccc ggggacagtc cccaatggtc agccggaacg ttgcgcctgc ggtagatcag
     2041 gtagagaaag aaaccggtgg cggccagaat gatgatcccc aaaaaatcgc ccgcctcgag
     2101 taaatctttg gggagcggga tgaagatggc ggttaggccg atggccaacg ccagccaaac
     2161 tccgtccagg agacgctcca ccacgatgga gggaatgacg gtcgcaaatt ctgcggaaat
     2221 ccagcgcgag actaagtagg cccggacgaa ttcgccgatg cgcatcggca ggatttcatt
     2281 ggtgaagagg ccggcataca cggcctgggt tgcccgcccg atcggcagcg tccccaccgg
     2341 ccgcaaaagg agttgccagc gcagaccctg gcagacgtaa ctcagggtgt cgcagacgac
     2401 cgcggggacc acccaccacc agtcaatggc ggggagggtg cgccagagtt tttccacccg
     2461 gatgtcgtag aaaacccaca ccagacctgc aaccgccaaa aggtagacga agaccgattg
     2521 gaaaaaccgc cgggcggatt tcgccggcgg acggggaata atggtcagc
//
LOCUS       DBNGZD010000088         6471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1612697, whole genome shotgun sequence.
ACCESSION   DBNGZD010000088 DBNGZD010000000
VERSION     DBNGZD010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1612697"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..797
                     /locus_tag="AC16GM_02335"
                     /pseudo
     CDS             54..797
                     /locus_tag="AC16GM_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
     gene            922..1767
                     /locus_tag="AC16GM_02340"
     CDS             922..1767
                     /locus_tag="AC16GM_02340"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR2922544.1"
                     /translation="MSLSNRDTPTLLNIDPKVTTEILTAFLVNEAGKFGFTKAVVGVS
                     GGLDSATVAILAARAYGPNNVLGVLLPYKHSSPDSLKDGLALVRQTKIRHEVVDITPL
                     AEPYIERFKAIDRVRIGNVLARLRMLVLFDLSHRGASLVMGTSNKTELLLGYSTWYGD
                     MASSLNPIGDLYKSQVRQLAAFLKVSKPIQKKIPTADLWVGQSDEGELGFTYDEVDRL
                     LYYLIDELATREQLVSLGFRESLVKRVERMIYNSQFKRSLPIICKLSRRTVGIDFQLS
                     KDWGR"
     gene            1788..2483
                     /gene="rsmI"
                     /locus_tag="AC16GM_02345"
     CDS             1788..2483
                     /gene="rsmI"
                     /locus_tag="AC16GM_02345"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR2922545.1"
                     /translation="MLYLVATPIGNLEDISYRALEVLKRVDLIAAEDTRHTGLLLKHF
                     GIQARMVSYHEHNEARRAAELLELLKSGKSIAIVSDAGTPGISDPAYRLVQSAIQDQI
                     PVVPIPGATAFVPALVASGLPTDRFVFEGFLPVKSGRRRKRLEELRTEERTIIFYESP
                     YRLVRLLGELQEVLGDRKAVVAREVTKKFEEFVRGPLSEIRAHFQLHPPKGEMVVLVA
                     GTKNAVEPGSRAE"
     gene            2480..3439
                     /locus_tag="AC16GM_02350"
     CDS             2480..3439
                     /locus_tag="AC16GM_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR2922546.1"
                     /translation="MNRVKPHNGWFFLPVDWAILIYLGSISLAMLFFWKNVPHWPLFL
                     TFNLAVSGLAFSLARQVGNPQRGLARFFRHWYPLFLFTFFYEETGPLVHIFFPNWFDM
                     DVVHLERVLTGGAPSVWLEQFASRPLSEFLMFGYFSYYLLLPVVGLILWLKRREQEFD
                     RMIFIAAVTFFVCYWGFYLYPVQGPRWAIGDWHQVPIRGWIFVPVVNFFINLAVRGGA
                     MPSSHVAVALVVLLFAWRNVRWLGIILTPIVAGLAVGTVYGRFHYAADVVVGIAIGWG
                     CDFVLNRWLARRAVREKLPAFVPGRMAPARPAFAERRSGAAPE"
     gene            3441..4256
                     /gene="lgt"
                     /locus_tag="AC16GM_02355"
     CDS             3441..4256
                     /gene="lgt"
                     /locus_tag="AC16GM_02355"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR2922547.1"
                     /translation="MHPEIIKLGPFALRSFGLFLALSFFAGLGLIKWRAKKYGTDFNR
                     LVNLAFVVIISGILGARLFYVFSHWSAFAGHPLDIINPFQSGQVVGIAGLTLYGGVIL
                     AIVCGSIYLKAARLPFWPTFDIFAPAIAFGIFLTRIGCFLNGCCFGMPTALPWGVHFP
                     ADSIPAYYFPGATLHPAQLYASAYGLLLFLVLVPLDNRKKFYGSTFAWFLIGESIFRF
                     LLEFVRYADPGTFFTLDGSRWTYNQLVAVGLFAGGVAMRIFLRKSFPLTVQKA"
     gene            4402..5100
                     /locus_tag="AC16GM_02360"
     CDS             4402..5100
                     /locus_tag="AC16GM_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR2922548.1"
                     /translation="MDKVQPTAAAGELGSDDQVISFKENLKRGAHAALDPLVRALARL
                     GMRADHLTILGLVFGLGAGVLFFAGRFRLGALLAALAGLCDLLDGQVVRLTKSVSRFG
                     AFLDSTLDRLAEAAILVGIAGFYIAHPEVLATGPEPGSWAVAVSLFAMLALVGSFLVS
                     YTRARAEGLGIECKVGWFERTERMVLIIIAGLFGVGPVMPGALVVLVLLSFATAAQRV
                     AYVWKKTHVAGLDR"
     gene            5115..6218
                     /locus_tag="AC16GM_02365"
     CDS             5115..6218
                     /locus_tag="AC16GM_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR2922549.1"
                     /translation="MSKVRVAIIGVGNCASSFVQGVEYYKNAKDGDFVPGLMHVNLGG
                     YHIRDIEFSAAIDIDKNKVGKDLSEAIYTKPNNTYKFCKVPNLGVKLVRGMTHDGLGL
                     YLSKIIEKAPGPTADIVKTLKDTGTDVVVNYLPVGSEEATKWYVEQVLDAGCGFVNCI
                     PVFIAREKYWQKRFRDRGLPVIGDDIKSQVGATILHRVLARLFRDRGVKLERTSQLNV
                     GDNTDFLNMLERSRLESKKISKTNAVTSQLDYNLGEENVHIGPSDYVAWLTDRKWAYI
                     RLEGRTFGDVPLNIELKLEVWDSPNSAGVVMDAVRCCKLAMDHKLSGAIEAPSAYFKK
                     SPPVQYTDEEARRMTEEFIQKYGRKAKPARVKK"
BASE COUNT         1238 a         1779 c         1982 g         1472 t
ORIGIN      
        1 aaacccacct cgcgctcatc cgcaaagccc gggcgcggaa ggcagacctc atcattttcc
       61 ccgaactatc cttaagcggc tacctgctca aggatatggt gccccgggtg gcgctgaaac
      121 tcgacgcgcc ggaagtcaag caacttctga cggagagcaa gtcgatagcc attgcggtcg
      181 gtctggtaga ggaaacgccg gaatcactct aattcaacag cgccctcttt ttggaaaaag
      241 gcaaaatggc gtattgccac cgcaaagtat acctgccgac ctacggcatg ttcgacgagg
      301 ggcgtttttt cggcgccggt gaagcgctgc gcgccttcga cacccgcgcg gggcggttgg
      361 gggtccttat ctgcgaagag gcctggcatt cattctgtcc ctaccttttg accttggacg
      421 gtgcggtggc cgtcatcaac atggccaaca gcacggcgcg ggggttggac ggaaaaaggc
      481 gggtgggcac ggctcagacc tgggagttga tgaaccgctt ctacgccggg atgcaccagg
      541 tgtttttcat tttcgtcaac cgcgtcggct tcgaagacgg cataggcttc tggggcgggt
      601 cggaagtgat cgatcccttc ggcaaggtgg cggccaaagc gccctacttc cgcgaacagt
      661 tagtttttgc cgacatcgac ttggggcagg tggagcaggc gcgcctgcgc acgccgcttc
      721 tgcgcgacga gcggctcgat ttctctttag ccgaattggc ccgggtgcgc gaccagcggc
      781 agaccaccct cgcttaatcc caagagaaaa atgcgtgtcg gggtgtcgaa gtatcgaggt
      841 gtcgaggttg gcttcaagcg tagtaggaat ttgcctttaa cttcgacact ccgacactct
      901 ccctcgactt cgctcggggc ggtgagcctg tcgaaccgcg acacgccgac acttttgaac
      961 attgatccga aagttacgac cgaaatcctc accgcttttt tagtcaacga agcgggaaaa
     1021 ttcggcttca ccaaagcggt ggtgggcgtc tccggggggc tcgattcggc cacggtggcg
     1081 attttagcgg cccgggccta cgggccgaat aacgtgctcg gcgtactctt gccctacaaa
     1141 cactccagtc cggacagttt gaaagatggc ctggctttgg ttaggcagac gaaaatcagg
     1201 cacgaggtgg tggacatcac tcccctggcg gagccgtaca tcgaacgatt caaagcaatc
     1261 gaccgcgtcc gcattggtaa cgttctggcc cgcttgcgga tgctggtcct cttcgacctc
     1321 tcccaccgcg gcgcgtccct ggtcatgggc acaagcaaca agaccgaatt gcttttgggt
     1381 tacagtactt ggtacggcga catggcctcc tcgctcaacc ccatcggcga cctgtacaag
     1441 agccaggtgc gtcagttggc ggctttcttg aaagtatcca agccaatcca gaaaaaaatt
     1501 cccacggcgg atctctgggt ggggcagtcg gatgaaggcg aactcggttt cacctatgac
     1561 gaagttgacc ggctcctgta ttacctgatt gacgaactgg ccacccggga acaattggtg
     1621 tccctcggtt ttcgagagtc actcgtgaag cgggtggagc ggatgatcta caactcccag
     1681 ttcaagcggt cgctccccat aatttgcaaa ctctcccgcc ggacggtggg gattgatttt
     1741 cagttgtcga aggactgggg gagataatcc cgaagttgtg gacgtgaatg ctttacttag
     1801 tcgccacccc cattggcaac ctggaagaca tttcttaccg tgcgcttgag gtattgaaaa
     1861 gagtcgacct gattgctgcg gaagataccc gccacaccgg ccttttgctc aagcacttcg
     1921 gtatccaagc ccggatggtg agttaccacg agcacaatga agcgcggcgg gcggcggagc
     1981 tgctcgaact tttaaagtcc ggcaaaagca ttgccatcgt ctccgatgcc ggcacgcccg
     2041 ggatttcgga cccggcctac cggctggtgc agtcagccat tcaggaccag attcccgtcg
     2101 ttcccattcc cggagccaca gcgtttgtcc ccgcgctggt ggcttccggc ctgcccaccg
     2161 accgatttgt tttcgaaggt tttctgcccg tcaaaagcgg gcggcgccgg aagcggttgg
     2221 aagagttgcg cacagaagag cgaaccatca tcttctacga atcgccctac cggctggtgc
     2281 ggcttttggg cgagttgcag gaagttttgg gcgaccggaa agcggtggtc gcacgggaag
     2341 tgaccaagaa gttcgaggaa tttgtgcgcg gaccgctatc agaaattcgc gctcactttc
     2401 agctgcaccc gccgaaaggg gaaatggtgg tcttagtggc agggaccaag aacgctgttg
     2461 aaccggggtc gcgagcggag tgaatagagt caaaccacac aacggctggt tctttctgcc
     2521 ggtggattgg gcgatcctca tctacctcgg ctcaatttct ctggcgatgc tcttcttttg
     2581 gaagaatgtc ccgcactggc cgctcttttt gacgttcaat cttgcggtga gcgggctggc
     2641 gttctccctg gcgcggcagg tggggaatcc ccagcggggc ttggcccgtt tcttccgcca
     2701 ctggtacccc ctcttccttt tcacattctt ctatgaagag accgggcccc tggtgcacat
     2761 cttcttcccc aactggttcg acatggacgt ggtgcatctg gagcgggtgc tcaccggcgg
     2821 ggcgccctcg gtctggctgg agcagttcgc ctcccggccg ctctcggagt ttttgatgtt
     2881 cggctacttc tcgtactacc tgcttttgcc ggtcgtcggc ctcatcctct ggctgaaaag
     2941 gagagagcag gaattcgacc ggatgatttt cattgcggcg gtcacttttt tcgtctgcta
     3001 ctgggggttt tacctctacc cggtgcaggg gccgcgctgg gcgataggtg attggcatca
     3061 ggtgccgatc cggggctgga tcttcgtgcc ggtggtgaac ttcttcatca acctcgcggt
     3121 gcggggcggg gccatgccct cctcccacgt ggcggtggcc ttggtggtgc tcctttttgc
     3181 ctggcgcaac gtccgctggc tgggaataat tcttacgccc attgtcgcgg gattggcggt
     3241 cgggacagtt tatggacgtt ttcactacgc tgccgacgtg gtcgtgggca tcgccatcgg
     3301 ctggggctgt gattttgtcc ttaatcggtg gctggcccgg cgggcagttc gcgaaaaact
     3361 tcccgccttc gtgccgggca ggatggctcc cgcacgtccg gcgttcgcgg aacgccggag
     3421 cggggccgcc ccggagtaac gtgcacccgg aaatcatcaa attagggccg tttgccctgc
     3481 gctccttcgg gctctttctg gcgctctctt ttttcgcggg tctgggatta ataaaatggc
     3541 gggcgaaaaa gtacggaacc gatttcaacc ggttggtcaa cctggccttc gtggtcatca
     3601 tctcaggcat cctgggagcg cggctcttct acgttttttc gcactggagc gcgtttgcgg
     3661 ggcatcccct ggacatcatc aatccctttc agagcgggca ggtggtgggg attgcggggc
     3721 tcacgctcta cggcggggtc attctggcca ttgtttgcgg atcaatctat ctcaaagcag
     3781 cgcggctgcc cttctggccc acgtttgaca ttttcgcgcc ggccattgcg ttcgggattt
     3841 tcctcacgcg catcggctgc tttttgaacg gctgctgttt cgggatgccg accgcccttc
     3901 cgtggggcgt gcattttccg gcagattcga ttccggccta ctactttccg ggcgccacgc
     3961 tgcacccggc gcagctgtac gcctcggcct acgggctttt gcttttcctt gttctcgttc
     4021 cgttagacaa ccggaaaaaa ttttacggca gcacctttgc ctggtttctc atcggcgaat
     4081 caatctttcg cttccttttg gaattcgtcc gctacgccga ccccgggacg ttttttaccc
     4141 ttgacggttc ccgctggacc tacaaccagc tggtggcggt gggattgttt gcgggcggtg
     4201 tggcgatgcg catttttttg agaaagagtt ttcccttgac ggtgcagaag gcctgactat
     4261 attttgcgat tccgattcgc acctgaaaaa tctcagaaca ggcggatagg gaagagtatc
     4321 ttttcatgta cacagagtgc cggtcagacc gtgacctctt ggcggtgacc tccagttcgg
     4381 ggagatcaaa ggcgttggag aatggataaa gtgcagccga cggctgcggc aggggagctg
     4441 gggtcggacg atcaggtgat ctcgttcaag gaaaacctga aacgcggggc gcacgcggct
     4501 ctggatcctc tggttcgggc gctggcacgg ctggggatgc gggccgatca cctgacgata
     4561 ctgggactgg tattcggtct gggtgcggga gtattgtttt ttgctgggcg gtttcggctc
     4621 ggcgcgcttt tggcagcttt ggccgggttg tgcgacctgc tcgacggcca ggtggtgcgc
     4681 ctgaccaagt cggtgtcccg ctttggagcg ttcctcgatt ccacgctcga ccggctggcc
     4741 gaggcggcca ttctggtagg cattgccggg ttctacatcg cgcacccgga ggtgctggcg
     4801 accggcccgg agcccggaag ctgggcggtt gctgtctcac tttttgccat gctcgcgctg
     4861 gtggggtcat ttttggtttc ctacacccgg gcgcgggccg aaggattggg gattgagtgc
     4921 aaggtcggct ggtttgagcg gacggaacgg atggttttga tcatcatcgc gggattgttc
     4981 ggcgtcgggc cggtgatgcc gggcgcgctc gtcgttttgg ttttactgtc gttcgccacc
     5041 gcggcgcaac gggtggccta cgtttggaag aagacccacg tagcaggttt ggatcgataa
     5101 ggaggttcgt tttcatgagt aaagtgcgtg tggcaattat tggtgtgggc aattgcgctt
     5161 cgtcgttcgt ccagggagtg gagtactata aaaacgccaa agacggcgac tttgtgccgg
     5221 ggttgatgca cgtcaatttg ggcggctacc acatccggga catcgaattc tccgcggcga
     5281 ttgacattga caagaataaa gtcggcaagg atttgagcga ggcgatctac accaaaccga
     5341 acaacaccta caagttttgc aaagttccaa acctgggcgt caaactggtg cgggggatga
     5401 cgcacgacgg gttagggctg tacctctcca aaatcatcga aaaagcgccg gggccgacgg
     5461 ccgacatcgt caagacactc aaggacaccg gcaccgacgt ggtggtgaac tacctgccgg
     5521 tgggttccga agaggcgacc aagtggtacg tagagcaggt tctggacgca ggctgcggtt
     5581 ttgtgaactg cattccggtt ttcatcgcgc gggagaagta ctggcagaag cgcttccgcg
     5641 accgaggcct gccggtgatc ggcgacgaca tcaaatcgca ggtcggcgcg accattctac
     5701 accgcgtatt agcgcgtctc tttcgcgatc ggggcgtcaa attggagcga acgagccagt
     5761 tgaacgtcgg cgacaacacc gactttttga acatgctcga acgctcacgg ctggaatcca
     5821 aaaagatctc caagaccaat gcggttactt cgcaactcga ctacaatctg ggtgaggaga
     5881 acgtgcacat cgggccgtcg gactacgtgg cctggctcac cgaccgcaag tgggcctaca
     5941 tccggctgga gggccgcacc ttcggcgacg taccgctcaa tatcgaactg aaattagagg
     6001 tctgggactc gcccaactcc gccggcgtgg tgatggatgc ggtgcgctgc tgcaagctgg
     6061 caatggatca taagctctcc ggtgccattg aagcgccttc ggcctacttc aagaaatcgc
     6121 cgccggtgca gtatacggat gaagaagcgc ggaggatgac ggaggagttc atccaaaaat
     6181 atggcaggaa ggcaaaaccg gccagggtga agaagtaagc cctattcccg ggtttctgac
     6241 ccctccctca ctctcccctc gaaggggagg gaacattttg ggcgaggagg atacggagta
     6301 gtttatatag aagtatggga agaaggcgaa accggccaaa gccaagaagt aatcctcctt
     6361 ttgaccccac cctgccctcc cctacagggg agggaaaaat ttgcaaaagg tttcgtagtc
     6421 gaggattaca tcctcggcgc acatcgacag gttagcgttg cattaagaat t
//
LOCUS       DBNGZD010000089         2689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_431862, whole genome shotgun sequence.
ACCESSION   DBNGZD010000089 DBNGZD010000000
VERSION     DBNGZD010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_431862"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="AC16GM_02370"
     CDS             <1..1066
                     /locus_tag="AC16GM_02370"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR2922550.1"
                     /translation="FALNKQKGNIFLNSAIGFITPKFDVNDLGFQFRTDVINGHVAGG
                     YRWTKPGKFARRANLQAATFRNYDFDGNLTWSGYWHSGFIQFLNYYGLDWNVSYNPQS
                     VNNRRTRGGPLILNLPGWDAGISVESDDRKTWVFGAGASGGGGRSGSWGWGISTSIEW
                     KPAANVTFRLSPGFDRGREDAQWVGAFTDTLATATFGKRYVFAKLDQTSLSSSIRLNW
                     TFTPKLSLQLYVQPLISSGHYHGFKELARPKSYDFLRYGDGGSTFSEATHVADPDGPG
                     PANPIQLGNPDFNFKSLRGNAVLRWEYRPGSTLYLVWTQSRSDVESIGDFQFNHSVTR
                     LLDARPDNIFLLKIAYWWGP"
     gene            1242..1442
                     /locus_tag="AC16GM_02375"
     CDS             1242..1442
                     /locus_tag="AC16GM_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922551.1"
                     /translation="MTRRTRQRVLLGGAVIFILLLLLCRPWDWLGGPPPAMAEVRDVE
                     QLRVLFNQEIGAPRLILLLSPT"
     gene            1470..1802
                     /locus_tag="AC16GM_02380"
     CDS             1470..1802
                     /locus_tag="AC16GM_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922552.1"
                     /translation="MQKQILDKHPQAKLQVYAIWLPMRFTDKYSRAGARVLSDARVKH
                     FWDGEKVVGRWFAEQEGYRGVAWDIYFLYGAEADWNRAPAPLVSSGVTVFGKRKELLH
                     ALLPLMAD"
     gene            complement(1896..2642)
                     /locus_tag="AC16GM_02385"
     CDS             complement(1896..2642)
                     /locus_tag="AC16GM_02385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2922553.1"
                     /translation="MALPLEGKSALVTGSAWGIGRATALALADAGAGVVINYRTSEKA
                     ALEVVDAIRKKGGKSIAVQANVAEPGQVELLFERAQKELGTLDILVNNVGAFLLKPLS
                     ETTFTEWTALITSNLHTTFLCSKAALPGMRAKNWGRIINISFATADKITSAKRVSPYQ
                     IAKTGVLIFTKTLAAEEVKNGITVNSVGPGFVDTGRIAEAFREQMLKLSPLDRLITPE
                     EVARAVVFLTSPESAGITGTHLTVAGGWQV"
BASE COUNT          538 a          797 c          734 g          620 t
ORIGIN      
        1 ctttgcctta aacaagcaga agggcaacat ttttctgaac tccgctattg gtttcatcac
       61 tccgaaattc gacgtgaatg acctgggctt ccagtttcgc accgatgtca ttaacggtca
      121 cgtcgccggc ggctaccgct ggaccaagcc cggtaagttc gcccgccggg ccaatcttca
      181 ggcagccacg ttccgcaact atgactttga cggcaacctg acctggtcgg gctactggca
      241 ttccggcttc attcaatttc tcaactatta cggtctggac tggaatgtct cttacaatcc
      301 gcaatcggtt aataaccgca gaacccgggg gggaccgcta atcttgaatc ttccgggatg
      361 ggacgccgga atctctgtag aatccgatga ccggaaaacc tgggtcttcg gcgcgggcgc
      421 cagcggcggt gggggtcgat ccggttcctg gggctggggg atcagcacca gcattgagtg
      481 gaagccagca gccaatgtca cgttccgact tagccccggc ttcgaccggg gtcgtgagga
      541 tgcccagtgg gtcggtgcgt tcacggacac tttggcaacg gcaacgttcg gcaaacgtta
      601 tgtcttcgcc aaattagatc aaacctcgct ttcatcgagc atccggctga actggacttt
      661 tactccgaaa ctgagtttgc aactctacgt tcagcccctg atctcgtccg gccactacca
      721 cggtttcaaa gaactggccc gccccaagag ttatgatttc ctccgctacg gtgatggcgg
      781 atccactttt tcggaggcga cacacgtagc cgacccggac ggccccggcc ctgccaatcc
      841 aattcagttg ggcaatcccg actttaattt caaatctttg cgcggaaacg ccgtcttgcg
      901 ctgggaatac cgtcccggct ccacgctcta cctcgtttgg acgcagagcc ggtcggatgt
      961 cgaaagcatc ggcgacttcc aatttaatca ttcagtaacc cggctcctcg acgccaggcc
     1021 ggacaatatc ttcctgctga aaatcgccta ctggtggggg ccgtagcgtt ttattccgta
     1081 gccgaggatt tcatcctcgg cgccgcagac gaagctttca tcccaaggca atttgtgata
     1141 ttggttcaga aatggccgtt gactcttcac acatagatcc agaagattgt ctcgccagag
     1201 aggccttgaa ggtttcttgc aaatgacagc ggagtaaaac cgtgacgaga agaacgaggc
     1261 agcgtgtgct cttgggagga gcggtgattt ttatcctcct gctcctcctc tgccggccct
     1321 gggactggct cggcgggcct cccccggcca tggcggaggt gcgtgacgtc gaacaactcc
     1381 gggtcttatt caatcaggaa ataggcgctc cccgtctcat cctgttgctt tcccccacct
     1441 gagccgcctg cgtcgaaggc gcccggtggg tgcagaaaca gatcttagac aaacatcccc
     1501 aggcgaaact gcaggtttat gccatctggc ttcccatgcg attcactgac aaatactccc
     1561 gagccggcgc acgggtgctt tccgatgcca gagtgaagca tttctgggat ggagagaaag
     1621 tagtgggccg ctggttcgct gaacaagaag ggtaccgcgg cgtagcctgg gatatttatt
     1681 tcctgtacgg tgcggaagcg gactggaacc gtgccccggc accgctcgtg agttcaggtg
     1741 tcaccgtttt tggaaagcgc aaggaacttc tccatgccct cctgcccctg atggctgact
     1801 gatagcttcc tcagcagata atctgatccg cttaagccgc tctcaaaatc cgtagtcagc
     1861 cctgtgtgct gtattctgtc tactgtgttc tgcctttaca cctgccaccc ccccgccacg
     1921 gtcaaatgcg tccccgtaat ccccgccgac tccggactgg tcagaaagac caccgcccgc
     1981 gccacctctt ccggggtaat gagccggtca agcggtgaga gcttgagcat ttgctccctg
     2041 aatgcctcgg caatgcggcc ggtatcgacg aaccccgggc cgaccgagtt cacggtgatg
     2101 ccgtttttca cttcttcggc tgcgagcgtt ttcgtgaaaa tgagcactcc cgtcttggca
     2161 atctggtaag gagagacccg cttggcggag gtgatcttat ccgccgtggc aaaactgatg
     2221 ttgatgatcc gtccccagtt tttggcgcgc attcccggca gtgcggcttt ggagcagagg
     2281 aaggttgtgt ggaggttgga agtgataagg gccgtccact cagtaaatgt cgtctccgaa
     2341 agcggcttga gcagaaacgc gccgacgttg ttcaccaaga tgtcgagggt gcccaattct
     2401 ttttgcgccc gctcaaagag gagctcaacc tgccccggct cggccacgtt cgcttgaacg
     2461 gcgatcgact tgcctccctt tttgcgaatc gcgtctacaa cttccaacgc tgccttttcg
     2521 ctggtgcggt agttgatgac gacaccggcc ccggcgtcgg ccaaggccag ggcggtagcc
     2581 cggccaatgc cccaggcgct gccggtcacc agtgctgatt ttccttccaa tggtagagcc
     2641 atactatctc cctctcctcg ggacgcttct ttactgaagg gaatctaca
//
LOCUS       DBNGZD010000090         4451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1821348, whole genome shotgun sequence.
ACCESSION   DBNGZD010000090 DBNGZD010000000
VERSION     DBNGZD010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1821348"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2589)
                     /locus_tag="AC16GM_02390"
     CDS             complement(<1..2589)
                     /locus_tag="AC16GM_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014544.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HIR2922554.1"
                     /translation="MKPRRLAALLIVFLLSAPGLSAYAPGRPISGQPGTATQRATVNF
                     ADIARREAAAPATTEELQDVPYPLPGPRNRQLPPGAVKGKDESSSLNRVNAPALTTVS
                     PPPAASFAALGDNNTRIPPDVHGAAGPSHLMTTLNSQVRIQNRTGGVISTVSLNSFWS
                     SLSSPSAFDPKILYDPYRNRWIFTACSNSRSSSSSVLIGVTQTSDPTGTWNLYRIDAD
                     PANVAWADYPSFGFNNNWIVVQMNLFAVANDAFIRSNIYAFNKADLYAGGTGLFTLLV
                     DTVGGFSQAPAITYDTTLSTMYLVEDWFGNSGGNGMLRICTITGPVGSEVYTPDVSFP
                     TVADPWEDVGSTGFTDFAPQSGSVQKIMCNDARMQDCVYRNGFLWCTHTVFLPAGSGT
                     RSGVQWWKLSTAGSILQRGRIEDPSGAVFYAFPSLAVNKNDDVVIGHSRFSASQFASG
                     NYSFRAAGDPSNTFQTDTVLKVGEASYYKTFSGTRNRWGDYTGTVVDPVNDIDFWTIQ
                     EYAATPSGGFDRWGTWWGKVVPPAVPCAADTVRPNLTCPANIVKSNDIGQCNAVVTYT
                     ATAFDNCPGVTLACSPSSGSTFALGVTDVKCVATDAAGNKDSCVFTVTVNDTQKPTVA
                     CPANITLGNDPGQCGAAVTYSATAADNCAGVSLVCTPLTNSTFPIGATPVKCAATDAA
                     GNKDSCTFTVTVNDAEPPVVNCPADITVSNDPGQCGAVVNFTALFSDNCPGTTLSFTP
                     ASGSTLAVGTTLVTCVATDGGGNKDTCTFNVTVNDTERPVAQCRDTVVIADSGQQAQV
                     VNLPTSVSDNCPGATIVCTPPSGSSFPAGLTGVVCTATDASGNTAACSLSVAVQCFRY
                     RGDGDES"
     gene            complement(3063..>4451)
                     /locus_tag="AC16GM_02395"
     CDS             complement(3063..>4451)
                     /locus_tag="AC16GM_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2922555.1"
                     /translation="GAGDVNGDGKADFIIGVPAATPGGLTNAGSAYVYSGATGALLYQ
                     KNGAGAHDRFGESVAGVGDVDGDGKVDFMIGAPHAYGASPNVGSAFVYSGASGLLLYQ
                     KNGTGYNDQLGSHVVGAGDVNGDGRADFIVAARLADVGGFGDAGSVYVYSGADGSLLY
                     QKNGPSSQAWLGSSVASTGDVNEDGKPDFIIGAPGVSPGGLSFAGSAYVNSGADGALL
                     FQIDGATAGSLLGLEVAGVGDVNDDGKADFGVGTFAPGFSDSGSVYVYSGVDGSLLYQ
                     KKHPTLGNGYWGISVAGVGDVNDDRKADFIIGVAGSSSAFVYSGADGALLFQIDGAAP
                     HDYFGYSVAGAGDVNGDGRADFIIGAPAPSFIYEPHIGSAYVYAFSCAPRGDVAGAPI
                     TNCPDGTIDIVDIVAVINNVVFGTPFCGPEGAADVGGDPQGCPDTIVDIVDIVSIINN
                     VVFGTPFCQACL"
BASE COUNT         1019 a         1269 c         1279 g          884 t
ORIGIN      
        1 gctctcatcc ccgtcccccc ggtaccggaa acactgcaca gccacgctga gcgaacaagc
       61 agcggtattc ccggacgcat ctgtagcagt acagaccacc cccgtgagtc ccgcgggaaa
      121 actggaaccg gaaggcggag tgcaaactat cgtggcgccg ggacagttgt ccgaaaccga
      181 agtgggcaag tttaccacct gcgcctgctg accggaatcg gcgataacta ccgtatcgcg
      241 gcactgcgcc accggacgct cggtatcgtt cacagtaacg ttgaaggtgc aagtgtcttt
      301 attccctcct ccatcagtgg cgacgcaggt aaccagtgtc gtccccactg ccaaggtcga
      361 accggaagcg ggcgtaaaag agagcgtggt gcccgggcag ttgtcggaaa atagagcggt
      421 gaagttcact acagcaccgc actggccagg atcgttactg acggtaatat cggccgggca
      481 gttgactacc ggcggctcag catcattgac ggttacggta aaggtgcaac tgtccttatt
      541 tccggcggca tcggtagctg cgcatttgac gggggttgcc ccaatgggga aagtcgagtt
      601 ggttaacgga gtgcaaacca gagaaacacc cgcgcagttg tccgcagcgg tagcagagta
      661 ggttaccgca gcgccgcatt ggccggggtc gttaccaagt gtgatgttgg ccgggcaggc
      721 gacagtcggt ttctgggtat cgttgacggt caccgtaaag acgcaactgt ccttatttcc
      781 ggcagcgtcg gtggccacgc atttaacatc ggttactccc agcgcaaaag tggagccgga
      841 agaggggctg cacgctaagg taactccggg gcaattatcg aaagcggttg cggtgtaagt
      901 taccacggca ttacactgac cgatgtcgtt gcttttgacg atgtttgccg gacaggtcag
      961 gttcggtctt accgtatcgg cggcacaggg aactgccggc gggacaacct ttccccacca
     1021 ggtgccccag cggtcaaatc cgcccgaggg agtggcggca tactcctgaa tggtccagaa
     1081 atcgatgtcg tttaccgggt cgacaaccgt gccggtgtaa tctccccagc ggttgcgggt
     1141 gccggaaaag gttttgtaat aagaggcctc gcccactttc agcacggtgt cggtttgaaa
     1201 ggtgttggac gggtcgcccg ccgcgcgaaa agagtagttg ccgctggcaa attgcgaggc
     1261 cgaaaagcgg gaatggccga tgaccacgtc atcgtttttg ttgacggcta agctgggaaa
     1321 agcgtaaaaa acagccccgc tcgggtcttc gattctgcct cgctgtaaaa tagagccggc
     1381 cgtggacagt ttccaccact gcacgccgga gcgggtgccg gagccggccg gcaaaaagac
     1441 cgtgtgcgtg caccagagga aaccgttgcg atagacgcag tcctgcatgc gcgcgtcgtt
     1501 acacattatt ttttgcaccg agccggactg gggagcaaaa tcggtaaagc ccgtggagcc
     1561 gacatcctcc cacgggtcgg ccactgtggg gaaagaaacg tccggcgtgt aaacttccga
     1621 ccctaccggg ccggtgatgg tgcagatgcg caacatgccg ttgccgcccg agttgccgaa
     1681 ccaatcctcc accagataca tggtggaaag ggtggtatcg taggttatgg ccggcgcctg
     1741 cgaaaatccg cccaccgtat cgaccagaag cgtgaagaga ccggttcccc cggcgtagag
     1801 gtcggctttg ttgaaagcgt agatattgga gcggatgaaa gcgtcgttgg ccacggcaaa
     1861 caggttcatc tgcaccacaa tccagttgtt gttaaaaccg aagctggggt agtcggccca
     1921 ggcgacgttg gccgggtcgg cgtcgatgcg gtataaattc caggtgccgg tcgggtcgct
     1981 ggtttgcgtc acgccgataa gcacggacga cgaggaagaa cgggaattgg agcaggcggt
     2041 gaatatccag cggttgcgat acgggtcgta caatattttc gggtcaaagg cgctggggct
     2101 gcttaaagaa gaccaaaaac tgttcagcga aacggtgctg atgacccctc cggtccggtt
     2161 ctggatgcgc acctgggaat tcaaagtcgt catcaaatgg ctcggcccgg ccgccccatg
     2221 gacatcggga ggaatccggg tgttgttgtc tcccagggcg gcaaaactgg cggcgggagg
     2281 cggagaaacg gtggtaagtg ccggagcgtt taccctattc agagaagaag attcatcctt
     2341 ccccttgacg gcaccgggag ggagttggcg attgcgcggg ccgggtagcg gatagggaac
     2401 gtcttgaagt tcttcggtcg tagccggagc ggcggcttcc cgccgggcga tatccgcaaa
     2461 atttactgtt gctcgctggg tggccgttcc cggctggcca gagatggggc gtcccggagc
     2521 gtaagcagaa agcccgggtg cgctgagcaa aaatacgatg agcaaagctg ccagtcggcg
     2581 tggtttcatc gaactattct cctctacatt cccggttgac ggaaaatcgg ccgttatcga
     2641 accctacaga gtaattatcg acctccacag tcgtcacgcg caatttgaga acgagtcgta
     2701 ggcggcgaat aaaaaaactg gttttgcact tccgctggcg gtttgagttc caccgacaat
     2761 cctaacgtgg ccgatcattc attattgtca aggatttttt cgtcctgaat caaaaaaatt
     2821 ccaatgctaa atctgtcagc caattacctt ccttctgggc ttcaatatac tgccaaatcc
     2881 ctgaaaaccg cactgtttga ggtcgcaggg ttaaaacgat tcccctcccc cgcgtcggcg
     2941 ggtcgaagat tcgtgccggg gcggggttta ccccgcccct ttttattttc cctctccgcg
     3001 taggagaggg tagggtgagg tcgaatgaat cttcttgatc cctgatctct aatctctgcc
     3061 cttcataggc acgcctggca gaacggtgtc ccgaacacca cgttgttgat gatggagacg
     3121 atgtccacga tgtctacgat cgtgtccgga cacccctgcg gatcgccccc cacatccgcc
     3181 gcaccctccg gcccgcagaa gggcgtcccg aagacaacat tgttaatcac cgccacgata
     3241 tccacaatat caatagtccc atccgggcaa ttcgtgattg gagcccccgc tacatccccg
     3301 cgtggcgcac aggagaaggc atagacataa gccgaaccga tatgtggttc gtaaataaag
     3361 ctaggagcgg gagcccctat tatgaaatcc gctctgccat ctccgttaac atctcccgct
     3421 ccagctaccg aatagccaaa atagtcatga ggggcagcac catctatctg gaaaaggaga
     3481 gcgccgtctg cgccggaata gacaaaagcg gaggaagacc ccgcaacccc aatgatgaag
     3541 tcagcttttc tgtcgtcgtt gacatccccg acaccggcga cggagatccc ccagtaccca
     3601 ttgccaaggg ttgggtgctt tttctgataa agcagcgagc cgtccactcc ggagtacacg
     3661 tacacagagc ccgaatctga aaaccctgga gcgaaagttc cgacgccgaa atctgctttg
     3721 ccgtcgtcgt tgacgtctcc aaccccggcc acctccagac ccaacagact accagcggtg
     3781 gcgccatcta tctggaaaag gagagctccg tctgctcccg aattgacata ggcggagcca
     3841 gcgaaactaa gcccgcccgg tgagacaccg ggcgccccga tgataaaatc gggtttccca
     3901 tcttcgttta catctcccgt tgaggctact gaactgccca gccaggcctg cgaggacgga
     3961 ccatttttct ggtaaagcag cgagccgtcc gctccggagt aaacatagac agaaccggca
     4021 tcaccaaagc cacctacatc tgcaagacga gccgcaacga tgaaatcggc tcgaccgtcg
     4081 ccgttgacat cccccgctcc gactacgtga ctgcccagtt ggtcattata tcccgtgcca
     4141 ttcttttggt aaagcaagag gccgctggca cccgaataaa caaaagcgga gccgacatta
     4201 ggtgaggccc cgtaagcatg gggagcccca atcataaaat caaccttccc gtctccgtcc
     4261 acatcgccca ctcccgccac agactcgccg aacctatcat gggcgccggc gccgtttttc
     4321 tggtaaagca gggcaccagt ggcaccggaa tagacgtaag cagaaccagc attagtgagc
     4381 ccgcccgggg tggcagctgg aacaccgatg atgaaatcag ccttgccatc tccgttaaca
     4441 tctcccgctc c
//
LOCUS       DBNGZD010000091         9296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1265155, whole genome shotgun sequence.
ACCESSION   DBNGZD010000091 DBNGZD010000000
VERSION     DBNGZD010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9296
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1265155"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..594
                     /locus_tag="AC16GM_02400"
     CDS             391..594
                     /locus_tag="AC16GM_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2922556.1"
                     /translation="MPVGKVKWFNDQKGFGFIAQNDGGPDVFVHFSAIQDEGFKTLRE
                     GDEVQFEIVKGPKGPQAANVVKP"
     gene            734..3739
                     /gene="secA"
                     /locus_tag="AC16GM_02405"
     CDS             734..3739
                     /gene="secA"
                     /locus_tag="AC16GM_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR2922557.1"
                     /translation="MFPALITKLFGSKHERDVKKLRPLVAQMNEIYATLQDLTDEQLA
                     AKTPEFKDRLAQGETLDDLLPEAFAVVKEACRRLCGKSWPVCGIEVTWNMVPFDVQLI
                     GAMILHQGKIAEMATGEGKTLAATMPLYLNALEGEGTHLVTVNDYLARRDAEWMGEVY
                     KLLGLSVGCIQNEMDPAERRTQYSCDITYGTNNEFGFDYLRDNMAVRLEDRVQRGFRY
                     AIVDEVDSVLIDEARTPLIISGPTNITTAEYGEMQPLVEQVVRKQTVLVNRLVAEAEE
                     LLKNDGEYDAGTKLLAARRGAPKNKRLFKLLQEEGVKKLVSRVESDYMRDKKLHEIDD
                     LLYFFIDEKEHTAELNEMGREMLSPEDQKLFVIPDIVEGLHQIEADEALSPEEKIKKK
                     EELYRLHAERSQKVHATSQLIKAYSLYERDVEYVVQDGKIIIVDEFTGRLMPGRRYSD
                     GMHQAIEAKEGVRVEQETQTLATITLQNFFRMYDKLAGMTGTAVTEANEFFQIYKLDV
                     LSIPTNEPVRRIDYDDQVYRSRREKYNAIIEEITQYHNENRPVLVGTTSVEVSETLSR
                     MLKRQGIRHNVLNAKFHQQEAEIITQAGQAGAVTIATNMAGRGTDIKLGEGVVKHPNC
                     ALVELKPDVEPCPYLKELNCYERVPCGLHILGTERHEARRIDRQLRGRSGRQGDPGSS
                     RFYLSLEDDLMRLFGSERIASIMDRLGLEEGEVIQHRMVTKAIERAQKRVEAQNFAIR
                     KHLLEYDDVMNQQREVIYDRRLAALEKEDLQEEVLALIDQTVEEIINRHGGQERYVED
                     WDWTGLREEFRRVFLVEVRFKPEEFAKLTYPQIIEAYKNAALEVYRAKEATVGPEVMR
                     KLEKFAVLSTIDERWKENLYEMDQLKTGIGLRAYGQRDPLIEYKKEAYQTFSEMIDRI
                     DRETVELIYKLQVGAPQQPVRRRQPAMQTVHAEATGMGYAGEAQASESASGGQPGKPK
                     PVRVEQKIGRNDPCPCGSGKKYKKCHGAT"
     gene            3839..4510
                     /locus_tag="AC16GM_02410"
     CDS             3839..4510
                     /locus_tag="AC16GM_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR2922558.1"
                     /translation="MARNQVRRKKRNKHAIIKKRYRQKTVKRRIEAFFLDNIGRIATR
                     EQIIRVAKDPETEKIPENWHQRLSELRTDDGYTILTWRNRGDLRVSEYLMPTDQKRPA
                     TSKRVKIGPQAWQTVLRRANYRCEWEEGVDVCGLRDGEIDPIGGGTVRLTPDHKRPHA
                     VATEIDPNDPESWQALCGRHQVMKKNYWDHTTGWLNVSAIVQAASEGEKRKVYEFLQR
                     YFGDK"
     gene            4647..5375
                     /locus_tag="AC16GM_02415"
     CDS             4647..5375
                     /locus_tag="AC16GM_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR2922559.1"
                     /translation="MNTLSKIDEPVTTTILDPWYNKGVGGIRPDYHEWLISLLSATAK
                     ISDHILLWGFPEIVHRVLDHLPSGFKLVAWLTWYYKNCPSVIRGWRSSQLTCLHIAKA
                     SAQLYPEHFLNDAQLERQKRGKLRYMPGPLSVLETPLLVGFVGKNEQVGHPAQKPIKA
                     IEPLVLMTTKEGDLVLDPMCGSGTTGVVCLALRRRAILCDDSEKYLKMTEKRLTEFNI
                     STYNGCSTLNSQGELKFSSLDNLL"
     gene            5423..6454
                     /locus_tag="AC16GM_02420"
     CDS             5423..6454
                     /locus_tag="AC16GM_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050135 - NADP+ nucleosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR2922560.1"
                     /translation="MVDYVDLGTKDPHDRHRTYNVRMFSNSSHNGQIDLVFQAVFTVD
                     ALEKLNLPLDDKRTQAYFATRARSLLSPGQTKIDIKSNNCSELMPKAATMGKGENTIA
                     RLLHEVNRKPYETDPVISGRPIFTDEDIYVSTDFDFSLIDDVLNFFERERYIVRAGGH
                     VWKIHPIGTDFFREQVKQESVTLERFRTGIEVQEVTRTRDLSSKKVFIVHGHNEQYKS
                     ELARFLEKIGFEPIILHEQPSKGMTLIEKLEEYSDVGYAFILLTPDDVGGQKGKLLPR
                     ARQNVVFEFGMFVGKLGRDRVCCLYTGNVELPSDLEGLVYVQLKGSVNEVKLDIVKEL
                     RAAGYAVNI"
     gene            6585..8717
                     /locus_tag="AC16GM_02425"
     CDS             6585..8717
                     /locus_tag="AC16GM_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit alpha/beta"
                     /protein_id="HIR2922561.1"
                     /translation="MAVAPLRIAETLLSRKQLTELYRIIYLSRKLDDEEIKLKKQERI
                     YFQISGAGHEAVLAAAGMVLKPGYDWFYPYYRDRALALALGVTPLEMLLQAAGAADDP
                     ASGGRQMPCHFGKKDLNIVSQSSPVGTQFLQALGAAEGSWYLSVVKELQTKVHSYRKD
                     EVVYVSGGDGACSEGEFWEALNAACNTACGHQFPILFLIEDNAYAISVPVEIQTPGGS
                     ISKVIRGLADEGLLWMQEVDGTDPIKSYEVLSGAVAYIRRERRPALVQAHVIRPYSHS
                     LSDDEAAYRPKEEREDETRRDPIRTYRKYLIDEGLAVEEELAAIEQNVQEEIERAEEQ
                     ALTAAKPAKSSVTRHVYSEKIDPRSKQFDTQPEFSGDPQTMVTLINRCLADEMTRDLA
                     IVVFGEDVADASREKYLDRVGGKGGVFKLTAGLQKRFGHHRCFNSQLAEARIVGGAVG
                     MATRGLKPVVEIQFLDYIWPAFMQIANELSMMRWRSNGTWSCPLVIRASYGGYLRGGA
                     LYHSQCRESLFTHLPGVWVVIPSNALDASGLLRTAIRCDDPVIFLEHKHLYRQTYNRQ
                     PYPGPNYCIPFGKANLVREGKDVTIITYGALVNRSKLAADQVADQDISVEVLDLRTLI
                     PFDWEAIAASVRKTSRVIVAHEDSLTSGFGAEIAARIVEQLFDYLDAPVMRIGALDTP
                     VGYSPELEEAILPQTGTIAETIRKITAY"
     gene            8752..>9296
                     /locus_tag="AC16GM_02430"
     CDS             8752..>9296
                     /locus_tag="AC16GM_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922562.1"
                     /translation="MKRALETIFVPFLIVSCIATGILGLGNPSGKPGSQPVPQAAAAV
                     PQSPVSTGSEDSSDFDENNWCGWRPDGLQRALELHARFQSLWPKRPLAPTLSATGNVD
                     RGNIAVIEDDGFILADAGSGLTLDDVQASRRFYATHPDSFDIIAIYTTADINLGGAFA
                     YERNISNEVSGIGLGIFDGSAG"
BASE COUNT         2352 a         2334 c         2676 g         1934 t
ORIGIN      
        1 cgatcaggtg acgcttaccc ggtcgctgga ggggcggctg gagctctaat cggggaagcc
       61 gggcggtcag gcggccaggg gggtctcaag gaacaaaggg cttctgccga taaatttaaa
      121 tggtgccacc ggcaaaaaag gacttgacaa attcggcggc agcacgtata tagggttaag
      181 aacaggttgg ttacaggttg aaggaagagg aaaaagaaca aactgcaaaa ccgtaaaacc
      241 ccgccgtggg cggggtggta cggctttttt gtaaggccct cccaaattgc aatccagcac
      301 acatcgccac agcatatcaa gtttgagcgt cccgcagacg gcgggatgca ttggtgtttc
      361 acttataacc gtaaaggagg tgagcgatag atgccagtag gaaaagtgaa gtggttcaac
      421 gatcagaagg ggttcgggtt catagcccag aacgacggtg gtcccgatgt ctttgtgcac
      481 ttttcggcga tccaggatga gggcttcaag actctgagag aaggggacga ggtccaattc
      541 gaaatcgtca agggtcccaa aggcccccaa gcggcgaacg tggtgaagcc gtaacttcac
      601 ctgttcgtcc aagcaaaacc ctggataagc gattgtctgg ggttttttat ttcttttttt
      661 tagactccaa ctttttgtcc gtattccgag tgattatatt agacggttaa cgtaaggatc
      721 caaaaggaag acgatgtttc cagcgctgat aacaaaacta ttcggctcca agcacgagcg
      781 ggacgtcaag aaactgcggc cgctcgtggc gcagatgaac gaaatttacg ccacgctgca
      841 ggacttgacc gacgagcagt tggccgccaa gacgccggag ttcaaggatc gtctggctca
      901 aggggagacc ttagacgacc tcttgccgga ggcctttgcg gtggtcaagg aggcctgccg
      961 gcggctctgc gggaagtcgt ggccggtctg cgggattgag gtcacctgga acatggtgcc
     1021 gttcgacgtg caattgatcg gcgccatgat actgcatcag gggaaaatcg ccgaaatggc
     1081 gaccggtgaa gggaaaactt tggcggccac catgccgctc tacctgaacg ccttagaggg
     1141 cgaaggcact cacttagtca ccgtaaacga ctatttggcc cggcgtgacg ccgaatggat
     1201 gggggaggtc tacaaactcc tcggcctttc cgtcggctgc atccagaacg aaatggaccc
     1261 ggcggagcgg cgcacccagt acagctgcga catcacctac gggacgaata acgaattcgg
     1321 gttcgactac ctgcgcgaca acatggcggt gcggctggaa gaccgggtgc agcgcggctt
     1381 ccgctacgcc atcgtggacg aagtcgattc ggtcttaatc gacgaggcgc ggacgccttt
     1441 gatcatctcc ggccccacca acatcaccac cgccgaatac ggcgagatgc agcccttggt
     1501 ggaacaggtg gtgcggaagc agacggtgct ggtgaacaga ttagtggccg aggcggaaga
     1561 actgctgaag aacgacggcg aatatgatgc cggcaccaag cttttggcgg cgcggcgggg
     1621 cgcgcccaag aataaacgcc tgttcaaact tttgcaggaa gaaggggtga agaaattagt
     1681 ctcgcgcgtg gaatccgatt acatgcgcga caaaaagctc cacgagatcg acgacctgct
     1741 ctactttttc atcgacgaaa aggagcacac cgccgaattg aacgaaatgg gccgggagat
     1801 gctctccccg gaagaccaga agctcttcgt catcccggac atcgtggagg ggctgcacca
     1861 gattgaagcg gacgaagcgc tttccccgga agagaaaatc aaaaagaaag aagagctcta
     1921 ccggctgcac gcggaaaggt cgcagaaagt ccacgccacg agccagttga tcaaggccta
     1981 ctccctctac gagcgggacg tggagtacgt ggtgcaggac ggcaaaatca tcatcgtgga
     2041 tgaattcacc ggccgcttga tgccgggccg gcgctactcc gacgggatgc accaggcgat
     2101 tgaggccaaa gaaggggtca gggtcgagca ggagacgcag acactcgcca cgatcaccct
     2161 gcagaacttc ttccggatgt acgacaagct ggccgggatg accggcaccg ctgtgaccga
     2221 agcgaacgag ttcttccaga tttacaagtt ggacgtcctt tccatcccca ccaacgagcc
     2281 ggtgcggcgg atcgactacg acgatcaggt ttaccgcagc cggcgcgaaa agtacaacgc
     2341 catcatcgaa gagatcaccc agtaccacaa cgagaaccgc ccggtattgg tcggtaccac
     2401 ctcggtcgaa gtttccgaaa ccctttcccg gatgctcaag cggcagggga tccggcacaa
     2461 cgttttgaac gccaagttcc accagcagga ggcggaaatc atcactcagg ccggacaggc
     2521 gggcgcggtg accatagcca cgaacatggc ggggcgcggc accgacatca aattggggga
     2581 gggtgtggtc aaacacccca actgcgcgtt ggtggaactc aaaccggacg tggagccgtg
     2641 tccctatctc aaggaattga actgctacga gcgcgtcccc tgcggcctgc acatcttggg
     2701 caccgaaagg cacgaggcgc ggcggattga ccggcagttg cggggccgtt ccgggcgtca
     2761 gggcgatccc ggctcctccc gcttctacct ttctttggaa gacgacctga tgcgcctttt
     2821 cggctcggag cggatcgcct cgattatgga tcgcttgggc ctcgaagaag gggaagtgat
     2881 ccagcaccgg atggtgacca aagcgatcga gcgggcgcaa aagcgggtgg aagcgcagaa
     2941 cttcgccatc cgcaagcacc ttttggagta cgacgacgtg atgaaccagc agcgggaggt
     3001 gatctacgac cggcgcctgg cggcgttgga aaaagaagac ttgcaagagg aagtgctggc
     3061 gctcatcgac cagacggtcg aggaaatcat caaccgccac ggcgggcagg aaaggtacgt
     3121 cgaggactgg gattggaccg ggctgcgcga agagttccgc cgggtctttt tggtcgaagt
     3181 ccgctttaag ccggaagaat ttgcgaaact gacctacccg caaattatcg aggcctacaa
     3241 aaatgcggcg ttggaggtct accgggccaa ggaggccacg gtggggccgg aggtgatgcg
     3301 caagttagag aagttcgcgg tgctttccac catcgacgag cgctggaaag agaacctcta
     3361 cgaaatggat cagttgaaga ccggcatcgg gctgcgggcg tacggccagc gcgatccctt
     3421 gatcgagtac aaaaaagagg cctaccagac gttttcggag atgatcgacc ggatcgaccg
     3481 ggagacggtg gagctgatct ataagctgca ggtgggcgca ccgcagcaac cggtccgccg
     3541 ccgccagccc gccatgcaaa cggtgcacgc ggaagccacg gggatgggtt atgccggcga
     3601 agcgcaggcc tctgagtccg cctcaggcgg acagcccggc aagcccaagc cggtgcgggt
     3661 ggaacagaaa ataggccgga acgacccctg cccctgcggg tcggggaaga agtacaagaa
     3721 gtgccacggg gcgacgtgaa agcaaccact gaccacaggc agaaaatata ctttttgggt
     3781 atattgaaca ttgtgagtat ttgtctgcgg ggagacaacc gaacaccagg aagtctgcgt
     3841 ggctagaaat caagttcgca ggaaaaagcg gaacaaacac gcaataatca aaaaacgtta
     3901 tcgccaaaaa accgtgaaga ggaggataga agctttcttt ctagacaata ttggtagaat
     3961 cgcgacaaga gaacagataa taagagtcgc aaaagatcct gaaacagaaa agatacctga
     4021 aaactggcac caaaggctgt cagagctcag aactgatgat ggctatacaa ttctcacttg
     4081 gcgcaaccga ggcgacttga gagttagcga atacttaatg cctactgatc agaagagacc
     4141 agcaacttca aaaagagtca aaatcggccc acaagcttgg caaacagtgc tacgtcgagc
     4201 gaattaccgc tgtgaatggg aagaaggcgt tgatgtctgt ggtttaagag atggagaaat
     4261 cgaccctata ggtggcggaa cagtcagact tactcctgac cacaaaagac cgcatgccgt
     4321 cgctactgaa attgacccaa acgatccaga atcttggcag gcactttgcg gcagacatca
     4381 agtaatgaag aagaactatt gggatcacac aacaggatgg ctgaacgttt ccgctatagt
     4441 tcaggcagca tctgaaggtg aaaaaagaaa agtctacgaa tttctgcaac ggtatttcgg
     4501 agataaataa agcggagatg attttggcaa agagattctc gaaagagcgg aagcaatttc
     4561 tccattctca gtcacggagt agaatccgac ccacggtcgg ggcatttcaa aggaatggaa
     4621 ttttagtttt gccacctaat gacgtcatga atactctgag taaaattgat gagccagtaa
     4681 ctacaacaat tctggatcct tggtataata agggagtagg gggtattagg ccggactatc
     4741 atgaatggct gatatcactt ttgtccgcaa ccgctaaaat ttctgatcat attttacttt
     4801 ggggattccc cgaaatcgtt cacagagtgc tcgaccatct tccctccggt tttaagcttg
     4861 ttgcgtggct tacttggtat tataaaaact gtccctcagt tatacgagga tggcgatcta
     4921 gtcaactaac ctgcctacac attgccaaag cttcagccca attgtaccca gagcactttc
     4981 tcaatgatgc tcaacttgaa agacaaaaga gaggtaaatt gagatacatg cctggccctt
     5041 tgagtgtttt agaaacacca ctcttagttg gttttgtagg gaaaaacgaa caggtgggtc
     5101 atccagctca gaagcctatt aaggctattg agccactggt acttatgact accaaagagg
     5161 gagatcttgt tttagatccc atgtgtggct ccggcactac aggcgtagtt tgcttagctt
     5221 tgaggagacg agcgattctt tgtgatgatt cagagaaata tttaaagatg actgagaaac
     5281 gtttgactga attcaatatt tctacctaca atggctgtag tacattaaac tcacagggcg
     5341 aactaaaatt ttcgtctctt gacaatctat tatgagtgta attgctgttc gcctgattga
     5401 accgaataag gtgagaggtg tcttggtcga ttacgtagat cttggtacta aggatcccca
     5461 cgatcgacac cgcacttaca atgttcgaat gttttccaac tcatctcata atggacaaat
     5521 agacctagtt tttcaagcgg tatttaccgt cgatgctctt gaaaaattga atcttcctct
     5581 ggatgataag cgaacgcaag catatttcgc caccagggct cgatctttac tgtctcccgg
     5641 acagacaaag atagacatta agtctaataa ttgctcagag ttgatgccaa aggccgctac
     5701 tatgggaaaa ggagaaaata ctattgcacg actcctacac gaagttaata ggaaaccata
     5761 tgaaaccgac ccggttattt caggcaggcc aattttcact gatgaggaca tatacgtttc
     5821 cacagatttt gatttttcgc taatagacga tgtgttaaat ttttttgaac gagagcgata
     5881 tatcgtacgc gctgggggtc acgtttggaa gatacatccg attggaactg acttttttcg
     5941 tgagcaagtg aaacaagaaa gcgtcacact ggaacgattt cgaactggca ttgaggtaca
     6001 agaagtgaca agaactaggg atttgtcctc aaagaaggtt tttattgtac acggtcacaa
     6061 tgaacagtat aaaagcgaat tggctcgctt cttagaaaaa attggttttg agccaattat
     6121 tcttcatgaa caaccgagca aagggatgac tcttatcgaa aaattggagg aatattctga
     6181 tgtgggatat gctttcattt tactcactcc cgatgatgtg ggtggtcaga agggcaaact
     6241 tttacccaga gcaaggcaaa atgtagtttt tgaattcgga atgtttgtag gaaaacttgg
     6301 acgggataga gtgtgttgtt tgtacacagg aaatgttgag cttccttccg atttagaagg
     6361 gttagtttat gtccaactca aaggaagcgt caatgaggta aagttggata ttgtaaagga
     6421 actccgggcc gccgggtatg ccgtgaatat ttagagaatc ataaatagta gggtcttccc
     6481 cgaggccgac cgcccggctc cgggacgagc cgggctacca tcgcaaagaa tctgcgtttt
     6541 ttgcggaaat agattatatt gactccggac tttttgagaa tcccatggcc gttgcaccgt
     6601 tgcgtatagc agaaacgctc ctctcccgca agcaactcac ggagctttac cgcattatct
     6661 acctctcccg caagttagac gacgaagaaa tcaaactgaa aaaacaggag cggatttact
     6721 ttcaaatctc cggggcgggg catgaggcgg ttttggccgc cgccgggatg gtgctcaaac
     6781 ctggatacga ttggttttat ccctattacc gcgaccgggc gctggcctta gctttgggcg
     6841 taacgccctt agaaatgctt ttgcaggcgg cgggcgcggc ggatgatccc gcttccggcg
     6901 gcaggcagat gccctgccac ttcggaaaga aagacttgaa catcgtctcg cagtcgagtc
     6961 ccgtcggaac gcagttcctc caggcgctgg gcgcggcgga ggggtcgtgg tacctttcgg
     7021 tcgtcaagga actgcagacc aaagttcatt cctaccgcaa agacgaagtg gtctacgtct
     7081 ccggcgggga cggcgcctgc agtgaagggg agttctggga ggcgctcaac gccgcctgca
     7141 acacggcctg cggacaccag ttcccgattc tctttcttat tgaagacaac gcctacgcga
     7201 tttcagtgcc ggtggaaatt cagacgccgg gcgggtcgat ttccaaagtc atccgcgggc
     7261 tggcggacga agggctgctc tggatgcagg aagtggacgg gaccgacccg ataaaaagct
     7321 acgaggtgct ctccggagca gtggcctaca tccggcggga acggcggccg gctttggtac
     7381 aggcgcacgt gatccgcccc tactcgcatt cgctttcgga cgacgaagcg gcctatcgtc
     7441 ccaaagaaga acgggaagac gaaacgcggc gcgatccgat tcgcacctac cggaaatacc
     7501 tgattgatga gggcctggcc gtcgaagagg agctggcggc aattgagcag aacgtgcagg
     7561 aagaaatcga gcgggctgaa gaacaggcac tgaccgcggc caaaccagcg aaatcttcgg
     7621 tcacgcggca cgtttattcg gaaaagatcg acccccgctc aaaacaattc gacactcaac
     7681 cagaattttc aggcgacccg cagacgatgg tgaccttaat caaccggtgt ctggccgacg
     7741 aaatgacacg cgacctcgcc atcgtcgttt tcggcgaaga cgtggcggat gcctcgcggg
     7801 aaaagtactt ggatcgcgtc gggggcaagg gtggcgtgtt caaactgacc gccgggctgc
     7861 aaaagagatt cggccaccac cgctgtttca attcccaatt ggccgaagcg cggatcgtgg
     7921 gcggcgcggt cgggatggcg acgcgggggc tgaagccggt ggttgaaatt caattcctgg
     7981 actacatctg gccggccttc atgcagatcg ccaacgaact ttcgatgatg cgctggcgtt
     8041 ctaatggtac gtggagctgt cctttggtca ttcgcgccag ttacggcggc tacctgcgcg
     8101 gcggggcgct gtaccacagc cagtgccggg agagtctttt cacccacctg cccggcgtct
     8161 gggtggtgat tccctccaat gctctggatg ccagcggcct tctgcgtacc gcgattcgct
     8221 gtgatgatcc ggtcatattc ctggagcaca agcacctcta ccggcagacc tacaaccggc
     8281 aaccataccc cgggccgaac tactgcatcc cgttcggtaa ggcgaatctc gtccgggaag
     8341 ggaaggatgt gaccatcatc acctacggtg cgctggtgaa ccgctcgaaa ttggccgccg
     8401 accaggtggc cgatcaagac atatcggttg aagttttaga cctgcggacc ttgattccct
     8461 tcgactggga agcgattgcc gcctcggtca ggaaaacgag tcgtgtaatt gtggcgcatg
     8521 aagattcact cacttctggc ttcggcgctg agatcgccgc ccggattgtg gagcagttat
     8581 tcgactacct tgacgcaccg gtaatgcgca tcggcgcgct ggacacgccg gtcggctact
     8641 cgccggagtt ggaagaggcg attctgccgc agacggggac gatagctgag acgattcgaa
     8701 agataaccgc atactgaatc atggctgact gcgtcgggta agggagggag catgaaacgc
     8761 gctttggaga cgattttcgt tccatttctt atcgtcagtt gtatcgccac cggcattttg
     8821 gggctgggca atccatcggg aaagccgggc agccaacccg ttcctcaggc cgcggctgcg
     8881 gtgccgcaat ccccggtctc aaccggcagc gaggattcat ccgatttcga tgagaataac
     8941 tggtgcggct ggcggccgga cgggctccag cgcgccttag agctgcacgc ccggtttcag
     9001 agtctttggc ccaagcggcc gcttgcgccc accctttcgg cgaccggcaa cgtcgacagg
     9061 gggaacatcg cggtgattga agatgacggt ttcattttag ccgatgcggg gagcggtctt
     9121 accctggatg acgtgcaggc ctcgcgccgc ttctacgcga cccatccgga cagttttgac
     9181 atcatcgcca tctacaccac cgcggacata aatttgggcg gtgcgttcgc ctatgagcgc
     9241 aatatctcca atgaagtctc cggcatcggc ctggggattt tcgacggctc ggccgg
//
LOCUS       DBNGZD010000092         9926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1474115, whole genome shotgun sequence.
ACCESSION   DBNGZD010000092 DBNGZD010000000
VERSION     DBNGZD010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9926
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1474115"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="AC16GM_02435"
     CDS             complement(<1..1178)
                     /locus_tag="AC16GM_02435"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR2922563.1"
                     /translation="MTLSEKILSEHAGYAVRAGDLCLVEADFIFAHDLSGPLCISQIR
                     ELGIPKLKYPERVNFFLDHSSPPSTHIAATDHKLIRDFCHERNLKLYDIGSGIAHQVI
                     MEKIARPGDVIIGGDSHTCHVGVFGAFATGMGSTDIAIAMLLGRTWLKVPETLKFEIT
                     GKLRVGVYAKDIMLSIIGKISVSGATYKAMEFVGDTIDSMPLYERAIFPNMAIEAGAK
                     AGLCPSDDMTRQYLREFGREKDYKPIAPDPDAKYKQVFEINVTNLEPTVALPHSMDNV
                     APVTSDKCKDVPVHQVYLGTCTNGRFEDFREAARILKGKRIKPGTRLVCTPASRDVYM
                     KGLKEGLWEIFLESGGLVNSPGCGACPGVHTGILGDGENCISTLNLNFKGRMGNPKAF
                     "
     gene            1378..2265
                     /locus_tag="AC16GM_02440"
     CDS             1378..2265
                     /locus_tag="AC16GM_02440"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR2922564.1"
                     /translation="MAEPANPKEYRHTFRIAAKPERVFTALTDPKELEKWFAEHVSIE
                     PKPGGRFDFWGKHTLWGLPESSAAQKITAYDPPRRLGFTWELKGKPTTVTFTLSEKGA
                     ATEVTLIHDLSAFAGDRWHWYLLSDHWHLALPNLLYFVETGQPAYRVDFSKARGNVRL
                     DIDIIASAEKIFQSLTVPQEMDKWLSEKAEVELKVGGRYSFGWTIIVDGKKVPGGPTT
                     ITEIIPNRRLVHGWYWPGEDTGSTEVTWQLESRGKKTVVQLKHTGFVADRDGTDYYQG
                     WSAFLCRLKYLLERGKSWE"
     gene            2362..3051
                     /locus_tag="AC16GM_02445"
     CDS             2362..3051
                     /locus_tag="AC16GM_02445"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR2922565.1"
                     /translation="MRRVILAVFLGLLILAGVVYAQVSKQEAAPAGKTPWHLKATIIE
                     ACSCPLFCPCYFNLKPAHHYCQFNNAFNIEQGNLGNVKLDGMKVWLSGDLGGDFTTGK
                     AEWLVFTFEPTATPEQIDAGTKVLGSLYSAFKFPVLGIDKKQITWEIKGNKATAKMGD
                     GSGSIDLVAATGSDGKTPTVINNLPYWSAKSNSGFNLYKSKHHYKGHEKDFTFEDTNG
                     FTIGIETSGEM"
     gene            complement(3128..4420)
                     /locus_tag="AC16GM_02450"
     CDS             complement(3128..4420)
                     /locus_tag="AC16GM_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIR2922566.1"
                     /translation="MKTPTEKKLIYDWNAAVPKKRPAYNRKVTLADETLRDGLQSPSI
                     IDPPIATKIKILHLMENLGIQSCDIGLPGSGGRARADITELACEISRRRMKIKPFCAC
                     RTVIADIEPLLEISEKAGYPLEPAIFIGSSPIRIYAEEWTLDRMLQLTEEAVSFAVKH
                     GLPVMYVTEDTTRAHPSVIKKLYTTAIECGARRITVCDTVGYVTPVGIANLIAYVRKV
                     VKATGEKVKIDFHGHRDRGLDIPNALSAIAAGVNQVHATALGIGERVGNCAMDLLLVN
                     LKLEGVIKNDLSKLNEYCRTVAEAVGMPIPINYPVIGRDAFRTATGVHAAAVIKAKKK
                     GDHYLANRVYSGVPAELFGRQQEIEIGFMSGASNVNYWLIQRGIPPTPELVATILQTA
                     KDHNKILTEAEVLEVVHRFGPGRTAPASATFGSRSQFA"
     gene            complement(4485..5633)
                     /locus_tag="AC16GM_02455"
     CDS             complement(4485..5633)
                     /locus_tag="AC16GM_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR2922567.1"
                     /translation="MEFELTENQKVVRDLAREFAQKKLFPRAEEFDAREELDLALVRE
                     MGELGFLGLAIPEEYGGGGLDYLALVLAVEELSQGCASHTAVVSLHNSLFAHPVLAFG
                     TEEQKKRFLPPIARGEKFCAFALTEPGAGSDAGALETSARGEGDFYVLNGNKVYVTDG
                     NLADFILVYATVDRSLGHKGITAFMVEKTMPGFAVGTREKKMGYKASPTYELSFTDVR
                     VPKENIIGMVGEGFKIAMDSLNGGRIVIGAKALGLAEGAYRLALKYAKERRQFGQPIA
                     NFQAIQFMLADMATQIEAARALIYHAARLKDHGLPHTLEGAQAKLYASEMATRVTHKA
                     MQILGGYGYLRDYQVERYYRDARVTEIFEGTSEIQRLVIARNLLKGKA"
     gene            complement(5682..6239)
                     /locus_tag="AC16GM_02460"
     CDS             complement(5682..6239)
                     /locus_tag="AC16GM_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922568.1"
                     /translation="MLWATVPTGRAQELPSGTSRLATEKPQITITDQFQKRISGGYYG
                     LTEDSILLHVSTRLGLRRIWVDDITHLKVKGKSQRGTGVLLGFLAGVAVAAIIDIQRI
                     QRSTEENFTDPAFPLKAAIAATIGGTVGYALGSAVHAKSEQMDLAPLPHQDKVDLFEI
                     LGETGHLPEWDNETTPAELDSRQKR"
     gene            complement(6284..7066)
                     /locus_tag="AC16GM_02465"
     CDS             complement(6284..7066)
                     /locus_tag="AC16GM_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR2922569.1"
                     /translation="MELKNVKFDREESLAVVTIDRPAVRNALNDETIAELTYVFGQIE
                     KDEQIQVVIITGVGDKAFVSGADIGELRKVDTLSGKAKTNRGQGLLFKIENLPQVVIA
                     AVNGYALGGGCELLLACDIRIASDNAKIGLPEVGLGIIPGYGGTQRLSRLVGWGKAKE
                     LVFTGEIIDAGECLRVGLVEKVVPLAQLMGEAKKMAFTIASKGPLAVRAAKRAVNVAP
                     DVDLKTGCAYEAVEFAALCATEDKREGTTAFLEKRKPVFKGR"
     gene            complement(7181..8035)
                     /locus_tag="AC16GM_02470"
     CDS             complement(7181..8035)
                     /locus_tag="AC16GM_02470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016423138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2922570.1"
                     /translation="MTISKIGICGCGLMGSGVAEVCAKSGFKTIVREVDEPTLAKGKA
                     RIEGSLAKAVERGKATEEQKKQASANLSFTTKLDDFKDCDIVIEAIIEQMEEKKKLWK
                     ALDGVLKPQAICASNTSSLSIVEMAAVTKRPGKFIGMHFFNPVPVMKLVEIVRSIVTS
                     VETFETVKKFAADLGKTVIVAKDTPGFIVNLLLIPYLLDAVRAVEAGVGTKEDIDAGM
                     TLGCGHPMGPLTLLDFVGLDTTYYIAEAMFAEFKDTRYAAPPLLKQMVLAGYNGRKVG
                     KGFYAYGK"
     gene            complement(8101..8478)
                     /locus_tag="AC16GM_02475"
     CDS             complement(8101..8478)
                     /locus_tag="AC16GM_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2922571.1"
                     /translation="MSALVQKFGLILGRFCLAAIFVYSGVRKFMDLSGTAATLTKVGF
                     PIPMLFAVGAATFEVAGALSLVAGLQTRIGACLLIIFLIPTTFIFHPPGTQLIQFLKN
                     LGILGGLFIIVASGEKKIGQGQK"
     gene            complement(8530..8931)
                     /locus_tag="AC16GM_02480"
     CDS             complement(8530..8931)
                     /locus_tag="AC16GM_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922572.1"
                     /translation="MQRYAGAQAHHDRMIRFLAARLTELEYRDIRADLDGFVRPDLIA
                     GRKHEERPDLTCLDASGRLVIIEVETANTWDDQHVIGQWTLFRSAANYAGGEFHVAIP
                     KDCGVEDGRVLVENTLDILEIKAEQIWELEV"
     gene            9109..>9926
                     /locus_tag="AC16GM_02485"
     CDS             9109..>9926
                     /locus_tag="AC16GM_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR2922573.1"
                     /translation="MEHLTRILFELFAIFLAAKLAGEIFERLHQPAVIGEILAGILLG
                     PHLLGLVNPSEILDVFAEIGVIILLFTIGLQTKVEDLLRVGWISLFVGVLGIALPFIL
                     GFALLKALNYKTVEALFVGTAMVATSVGITARVFADIGVLKERTSQIILGAAVIDDIL
                     GMLFLAVVSGLGEGTISYVHLGIVFLEAAGFVLFLIFVGRRLIRRVVPEMNQFKSRNA
                     PFSLAILFCLGLSAMANYLQIAAIVGAFMAGMVLAEFNLVYGISVKSESLYDFLV"
BASE COUNT         2108 a         2878 c         2766 g         2174 t
ORIGIN      
        1 atgaaggcct ttgggttccc catccggcct ttgaaattca ggttaagcgt ggaaatgcag
       61 ttttcgccgt cgcccaaaat gccggtgtgg acgccgggac aggccccgca cccgggggag
      121 ttgaccaacc cgccagattc aaggaaaatc tcccacaacc cttccttgag ccctttcata
      181 tagacatccc gggaggcggg agtgcagacc aagcgcgttc cgggtttgat tcttttgcct
      241 ttgaggatgc gcgccgcttc gcggaaatcc tcgaaccggc cgttggtgca ggtgccgagg
      301 taaacctgat gcaccgggac atctttacac ttatcacttg tcaccggcgc gacgttgtcc
      361 atcgaatggg gcagagcgac agtcggttcg aggttggtta cgttgatttc aaaaacttgc
      421 ttgtatttgg cgtcgggatc tggcgcaatc ggcttgtaat ctttctcccg cccgaattcg
      481 cgcaggtatt ggcgggtcat gtcgtccgaa ggacagagcc cggccttggc cccggcttct
      541 atcgccatgt tcgggaagat ggcgcgctcg tagaggggca ttgagtcaat cgtgtcgccc
      601 acgaactcca tcgctttgta agtcgccccg gaaacggaaa tcttcccgat aatggagagc
      661 atgatgtctt tggcatagac ccccacccgg agtttgccgg tgatttcaaa cttgagcgtc
      721 tccggcacct tgagccaggt gcggcccagg agcatggcaa tggcgatgtc cgttgaaccc
      781 ataccggtgg cgaaagcgcc gaagaccccc acgtggcagg tgtgcgaatc gccgccgatg
      841 atcacgtccc ccggtcgggc gattttctcc ataatcacct gatgcgcaat gccggagccg
      901 atgtcgtaaa gtttcaggtt gcgctcgtgg cagaagtcgc ggattaactt gtggtcggtg
      961 gcggcgatat gcgtcgaggg cggcgaggag tggtctaaaa agaaattgac ccgttccgga
     1021 tacttgagtt tgggaatccc caattcgcgg atctgggaaa tgcagagcgg gccggagaga
     1081 tcgtgagcga agatgaagtc ggcttcgacc aagcagaggt cgcccgcccg gacggcgtag
     1141 ccggcgtgtt cggagaggat tttttcggat aaggtcattc ccataggcgc ctcgtgcgtt
     1201 gcgagaacca atatagggag cgaaaaacgg acgggcaatg accggtgcat gcgccggttg
     1261 actgtctcgg ggaatacgat aatttcgcct acaaacagaa tagaagccac cacggagaca
     1321 cagaggacac agagaaaaat cgatcctctc tatttctgaa cgaagaaaga atcgactatg
     1381 gctgaaccag cgaatccaaa agaataccgg cacactttcc ggatagcggc caaaccagag
     1441 cgggtcttca cggcgctcac cgacccgaaa gaactggaaa agtggtttgc ggagcatgtc
     1501 agcattgagc caaaacccgg tgggcgcttt gacttctggg gtaaacacac cctttggggg
     1561 ctgcccgagt cgagtgccgc acaaaagatt acggcttatg acccaccccg ccgcctgggc
     1621 ttcacctggg agttaaaggg caaaccgacc acagttacgt tcactctctc agaaaaaggc
     1681 gctgctaccg aagtcaccct gatccacgac ctctctgcct tcgccggtga tcggtggcac
     1741 tggtacctct tgagcgacca ctggcacctg gccttgccga atctcctcta cttcgtcgaa
     1801 actggtcagc cagcgtaccg cgtagatttt tccaaagcga gaggaaacgt gcgcttggat
     1861 attgatatta tagcctcggc ggagaaaatt tttcagtccc tcaccgttcc acaagaaatg
     1921 gacaaatggc tttctgaaaa agcggaagtg gaattgaaag tcggcgggcg ctactctttc
     1981 ggctggacga taatagtcga tgggaagaag gtccccggcg gcccgacgac aatcaccgag
     2041 atcatcccga acagaagact cgtgcacggc tggtactggc ccggcgaaga caccggttcc
     2101 acagaagtaa cctggcagct cgaatcgcgc ggcaaaaaaa cagtggtgca attgaagcat
     2161 accggctttg tggctgaccg cgacggaacc gactactacc agggctggag cgccttcctc
     2221 tgccggctca agtacctgtt ggaacgaggg aaaagctggg agtagagccc cccaaaaaag
     2281 ttgttgacaa attccagagc gataacgtat tctttcccgt cgtggaaaag cagaataaac
     2341 cttaaccaca aggagggatc gatgcgcaga gtaattctgg ccgtcttcct cggactgctc
     2401 attctggcag gcgttgtcta tgcccaggtg agcaaacagg aagcggcccc cgcgggcaaa
     2461 acgccctggc acctgaaggc gacaattatc gaggcctgct cgtgcccgct tttctgcccg
     2521 tgctacttca acctcaagcc ggcccaccac tactgccagt tcaacaacgc cttcaacatt
     2581 gagcagggga acctggggaa cgtgaagttg gacgggatga aagtctggct ctccggcgac
     2641 ctgggcggcg acttcaccac cggcaaggcg gagtggctgg ttttcacctt tgaaccgacg
     2701 gccaccccgg agcagatcga cgccggaacc aaggtgctgg gctcgctcta ctcggccttc
     2761 aagttcccgg tcttaggaat cgacaagaag cagatcacct gggagatcaa ggggaacaag
     2821 gcgaccgcca agatgggcga cggcagtggt tcgattgacc tcgtcgccgc caccgggtcc
     2881 gatggcaaga ccccgacggt catcaacaac ctcccctact ggtcggccaa gagcaacagc
     2941 ggcttcaacc tgtacaagtc caagcaccat tacaaggggc atgagaagga tttcaccttc
     3001 gaggacacca acgggtttac gatcgggatc gaaacctccg gagaaatgta aacccggggc
     3061 gtacgcctcc aaaggagagg gcaacctctc ctttttattt tagtctcaag gagtggggaa
     3121 tgacggctta agcgaactgc gatcgactgc caaaggtagc cgaggcgggc gcggtgcggc
     3181 cgggaccgaa ccgatgcacg acttccagca cctcggcttc ggttaaaatt ttattgtgat
     3241 ctttggcggt ctggagaatg gtggcgacga gttccggcgt cggcgggatg ccgcgctgga
     3301 tgagccagta gttgacgttc gaagccccgc tcatgaaacc gatttctatt tcctgctggc
     3361 ggccgaagag ttccgccggt accccggaat agacgcggtt ggcgagatag tggtcgcctt
     3421 ttttcttggc tttgatcacc gccgccgcgt gcacgccggt ggcggtgcgg aaggcgtccc
     3481 gcccgatgac cgggtagttt atcggaatcg gcatccccac cgcttcggcc accgtgcggc
     3541 agtattcgtt tagtttcgac aggtcgtttt taatcacgcc ttctaatttc aaattcacca
     3601 gaagcaaatc catcgcgcag ttccccaccc gctcgccgat gccgagcgcg gtggcgtgga
     3661 cttgattgac cccggcggct attgcggaaa gagcgttggg gatatccaaa ccccggtcac
     3721 ggtggccgtg gaagtcgatt ttgaccttct cgccggtggc tttgaccact tttctgacgt
     3781 aggcaatcag attggcaatc cccaccggcg ttacgtatcc caccgtgtcg cagaccgtga
     3841 tccgccgcgc cccgcactca atggcggtgg tatataactt cttgataact gagggatgcg
     3901 cccgggtggt gtcctcggtt acgtacatca ccgggaggcc gtgcttgacc gcaaaactca
     3961 ccgcttcttc ggtcaactgg agcatgcggt caagcgtcca ctcttccgcg tagatgcgga
     4021 tgggcgaaga gccgatgaaa atcgccggtt ccagagggta gccggctttt tcggaaatct
     4081 ccaaaagcgg ctcgatgtcg gcaatgaccg tgcggcaggc gcagaatggc ttgattttca
     4141 tccggcggcg cgaaatttcg cacgccaatt cggtaatatc ggcgcgcgcc cggccgcccg
     4201 aaccgggcaa accgatgtcg cacgactgaa tgcctaagtt ttccatcagg tggaggattt
     4261 ttatcttggt ggcgatgggc ggatcgatga tcgagggtga ctggagcccg tcgcgcaggg
     4321 tctcgtccgc tagggtgact ttccggttgt aggcgggacg ctttttcgga actgcggcgt
     4381 tccagtcgta aatcagcttc ttttcggtag gcgttttcat tgcggttcat caaatctacg
     4441 gttcgtagaa aagacaagca agtaactgtc tcggttaaca gtcgtcacgc ttttcccttg
     4501 agcaaattcc tggcgatcac cagccgttga atttcggaag tcccctcgaa aatctcggtc
     4561 acccgcgcat cacggtagta gcgctccacc tggtagtcgc gcaggtagcc gtaaccgcct
     4621 aaaatctgca tcgctttgtg cgtcacgcgg gtggccattt ccgaggcgta cagtttcgcc
     4681 tgggcgcctt ccagcgtatg cggcaaaccg tgatccttga gccgggccgc gtggtaaatc
     4741 aaggcgcgcg ccgcttcaat ctgggtcgcc atatcggcca acatgaactg gatggcctga
     4801 aaattggcga tcggctgacc gaactgccgg cgctctttgg catatttgag cgccagtcga
     4861 tacgcacctt cggccagacc caaagccttg gccccaatca caatccggcc gccgttgagg
     4921 gaatccatcg cgatcttgaa tccctctccg accattccga tgatattttc cttcgggacg
     4981 cgcacgtcgg tgaacgaaag ctcataagtc ggcgaagctt tgtagcccat ctttttttcc
     5041 cgcgtcccca cggcaaaacc cggcattgtc ttctcgacca tgaaagccgt aataccctta
     5101 tgcccgagcg aacggtcaac cgtggcatag acgagaatga agtcggccag attcccgtcg
     5161 gtgacgtaga ctttattccc gtttaagaca taaaaatctc cctccccgcg agccgaagtc
     5221 tccagagccc ccgcatcgga cccggcgccg ggttcggtga gggcgaaagc gcagaacttt
     5281 tccccgcgcg cgattggcgg caagaaacgc tttttctgct cctcggtccc aaaggccaga
     5341 accgggtgag cgaagagcga attgtggagc gacaccaccg ccgtgtggga agcgcaaccc
     5401 tggctcagtt cctccacggc taagactaaa gcgagataat ccaggccgcc gccgccgtac
     5461 tcttcgggaa tggcgaggcc gaggaagccc agctctccca tttcccgcac caacgctaag
     5521 tcgagttcct cccgggcatc gaattcttcg gcgcggggaa aaagcttttt ctgcgcaaac
     5581 tcgcgcgcca gatcgcgcac gaccttctga ttttcggtaa gttcaaattc catgtggttt
     5641 ggactgtccg tctccgccaa gaagtctctc aggacgactg cctatcgctt ttggcgcgaa
     5701 tccagttcgg ctggagtcgt ttcgttatcc cactcgggaa ggtgcccggt ctcgcccaga
     5761 atttcaaaca gatcaacttt atcctggtgg ggcaacggtg ccagatccat ctgttcggat
     5821 ttcgcgtgca ccgctgagcc gagggcgtat cccacggtgc cgccgatggt ggccgcgatg
     5881 gccgccttga gcgggaaggc cggatcggta aaattctcct cggttgaccg ttggatgcgc
     5941 tggatgtcta tgatcgccgc caccgccacc cccgccagaa aaccgagcaa aacacccgtt
     6001 cctcgctgcg atttcccttt gactttaaga tgggtgatgt cgtccaccca aatccgccgc
     6061 agacccagcc gggtggaaac gtgcaggagg atgctgtcct ccgtcagccc gtagtaaccg
     6121 ccagaaatgc gcttctggaa ctggtcggtg atggtaatct gcggcttctc cgtcgccaga
     6181 cggctggtgc cggacggcag ctcctgggcc cgtcccgtcg gcacggttgc ccaaagcaac
     6241 cagcccacac acgagacgca caaaaacaag cgtgacaatg tctttatcgc cccttgaaga
     6301 ccggtttccg cttctccaga aatgcggttg tcccttcgcg cttgtcttcc gtggcgcaga
     6361 gcgcggcaaa ttccaccgcc tcgtaagcgc agccggtttt gaggtcgacg tctggtgcca
     6421 cattgaccgc ccgcttggcc gcccggaccg ccaggggacc tttactcgct atggtgaagg
     6481 ccatcttttt cgcttctccc attaactgcg ctagaggcac gactttctcc accagtccaa
     6541 cacgcagaca ctcgccggca tcgataatct cgccggtaaa gaccaattcc ttggccttcc
     6601 cccagccgac caggcgcgaa agtctctgcg ttccgccgta gccgggaatg atgcccaacc
     6661 ccacctccgg caggccgatt ttggcgttgt cggaagcaat ccggatgtcg caggccaaaa
     6721 gcagctcgca cccaccgcct aaagcgtagc cgttgaccgc cgcaatcacc acctgcggca
     6781 ggttttcaat cttgaacagg agcccctgac cccggttggt tttggccttg ccggaaaggg
     6841 tatcgacttt ccgcaattcg ccgatgtcgg cgccggaaac gaaggccttg tccccgaccc
     6901 cggtaatgat caccacctgt atctgctcat ctttttcaat ctgaccgaag acgtaggtca
     6961 gctcggctat ggtttcatca ttgagggcat tgcgcaccgc ggggcggtcg atggtgacga
     7021 cagccaaaga ttcttcacgg tcaaatttga catttttcag ttccattgtt cctcctgcgc
     7081 gtggaaacag agcgggctcg cctgaagtcg agcatccagg atgaagaaaa ctaccggagg
     7141 ggtgacttga gccgccccat cactattatc cgactgtcta ctacttgccg taggcgtaga
     7201 atcctttccc gactttgcgg ccgttgtagc cggccaaaac catttgcttc aataatggcg
     7261 gcgccgcata acgggtgtcc ttgaattcgg cgaacatcgc ctcagcgatg tagtaggttg
     7321 tatccagccc cacgaaatcc agaagagtca aaggccccat cgggtgcccg cagcccaggg
     7381 tcatcccggc gtcgatatcc tctttggtcc ccacccctgc ttccacggcg cgcacggcgt
     7441 ccaaaagata gggaatgagc aaaagattca cgatgaaacc cggggtgtct ttggccacga
     7501 tgaccgtctt acccagatcg gcggcgaatt tcttcaccgt ctcaaacgtt tcaacgctgg
     7561 tgacgatgct gcgcacgatt tccaccaatt tcataaccgg caccgggttg aagaaatgca
     7621 tcccgatgaa ttttccgggt cgtttggtga ccgcagccat ctcaacaatc gaaagcgagg
     7681 aggtgttgga agcgcagatc gcttggggtt tgaggacgcc atccagcgcc ttccagagtt
     7741 ttttcttttc ctccatttgc tcgatgatgg cctcgatcac gatgtcgcag tctttgaagt
     7801 cgtcgagttt ggtcgtgaac gagagattgg ctgacgcctg ttttttctgc tcctcggtgg
     7861 ccttgccccg ctccaccgct ttggcaagcg acccttcaat gcgcgctttc cctttagcca
     7921 gcgtgggctc atccacttcc cggacgatgg ttttgaaccc gcttttggca cagacctcgg
     7981 caacgccgga tcccatcaaa ccgcacccgc agataccgat cttactgatc gtcattcagt
     8041 ctccttcctt atttttttcg ttctacccat tgcggatccg aaaaacggat tttctaattt
     8101 ttatttttgc ccctgaccaa tttttttttc tcctgatgcc acgatgatga ataaccctcc
     8161 cagaataccc aaattcttca aaaattgaat caactgcgtt cccggcgggt gaaaaatgaa
     8221 cgtcgtggga ataagaaaaa taatgagcag gcaggcaccg atacgggtct gcagaccggc
     8281 gactaaagac aaggcaccgg ccacctcaaa agtcgccgcg ccgacggcga aaagcatcgg
     8341 aatggggaaa ccaactttgg tgagtgtggc ggccgtgccg gaaaggtcca tgaatttacg
     8401 gacgccggag tagacgaaga tcgccgccag acagaagcgg cccaggatga ggccgaattt
     8461 ttgaacgagt gcactcatgg aatttcttcc ggttttaagc gaaccggcct taaggccggt
     8521 tctaaattct cacacctcta actcccagat ttgttcggct ttgatttcca gtatgtccaa
     8581 agtgttttca accagaaccc gtccatcttc caccccgcag tctttgggaa tggccacgtg
     8641 aaactccccg cccgcgtaat tcgcggcgga gcggaagagc gtccactggc cgatgacgtg
     8701 ctggtcgtcc caggtattgg cggtttcgac ttcgataatc accagccggc ctgaagcgtc
     8761 gaggcaggtt aggtcgggtc gttcctcatg cttgcgcccg gcgatcaagt cgggacgcac
     8821 aaaaccgtcc aggtcggccc ggatgtcgcg gtactccagt tctgtcagtc tggcggctag
     8881 gaatcgaatc atgcggtcgt gatgggcctg ggccccggcg tagcgctgca tcggaacatc
     8941 tccggtgcaa agtaaaagac aatccggggg aaatcaagac ggccgaacgc acggtgcgtc
     9001 caacgggtgt tggagtcatc cgatgcagga gaacttccgg tcgtttaata ccgaacaaat
     9061 ttccttgacc attcaattca ccggacggta tgttcactcc gtggggttat ggaacatcta
     9121 acgcgcatcc tttttgaact cttcgccatt ttcctggcgg ccaaactggc cggcgagatc
     9181 ttcgagcgcc tgcaccagcc ggcggtaata ggcgaaatct tagccggcat ccttttgggc
     9241 ccccacctcc tggggctcgt aaatccttcg gaaatcttgg acgtgttcgc cgaaatcggc
     9301 gtcatcattt tgctgtttac catcggcctg cagacgaagg tcgaagacct cctgcgggtg
     9361 ggctggattt cgctcttcgt cggcgtgctg ggaattgccc tccctttcat tctgggtttt
     9421 gcgctcctga aagcgctgaa ctacaaaacc gtagaagccc tctttgtggg cacggctatg
     9481 gtcgccacct cggtgggcat caccgcccgg gtttttgccg acataggagt gctcaaagag
     9541 cgcaccagcc agatcatcct gggcgcagcg gtgatcgatg acattctggg gatgcttttc
     9601 ttagccgtgg tctccggact gggtgaggga acgatctcct acgtccatct cggaatcgtc
     9661 tttctggaag cggcgggatt tgtcttgttc ttgatcttcg tcggccggcg cctgatacgc
     9721 cgggtggtgc cggagatgaa tcagttcaaa agccgcaacg cccccttttc gctcgccatt
     9781 ctcttctgcc tggggctttc ggccatggcc aactacctgc aaatcgccgc catcgtcggc
     9841 gcctttatgg ccgggatggt cttggccgaa ttcaacttgg tctacggcat ctcggtgaag
     9901 tcggaatcgc tctacgactt cctggt
//
LOCUS       DBNGZD010000093        11747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1613118, whole genome shotgun sequence.
ACCESSION   DBNGZD010000093 DBNGZD010000000
VERSION     DBNGZD010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1613118"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="AC16GM_02490"
     CDS             complement(<1..968)
                     /locus_tag="AC16GM_02490"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR2922574.1"
                     /translation="MSESYYNKIVSVQGKAAKMVGAALGLLILILFLWFGASPVKATV
                     PDGFVDTVIVSGLNQPVGFAFLPDGRILFIEQVTRRIRLVVQGAIAARDPLFTIADVN
                     TSGGERGLLGIAVDPGWPARPYIYCYFSHAGTATNYLVMFPVTGDLNDPTSDSLIISG
                     ASRYNILTDMPDNANNHNGGTLRFGSDGRLYLSLGEDAVPCAAQDSSGLRGMILRLSV
                     DSLPGAGAGPAPKSSITPSNNPFVASTNVNARLCYCFGLRNPFRFNIDPPTGDLFIAD
                     VGQNTWEEVSLSENGGENFGWPFREGPAVMIPGGCAEPGGPGAATYT"
     gene            complement(1196..1654)
                     /locus_tag="AC16GM_02495"
     CDS             complement(1196..1654)
                     /locus_tag="AC16GM_02495"
                     /inference="COORDINATES: protein motif:HMM:NF025725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HIR2922575.1"
                     /translation="MTYCIDTSALLAAWNERYPIINVPNFWNLFDQWVSQGKIISPIE
                     VRNEIKKKEDGLADWLKNREAMFVELEEDIQRAAREILVQFPLLTKGIEGRKIADPFV
                     ISLAKARGCTVVTEEGPGSPNRPRIPLVCRHFRTPCVNLLGLIQGENWVI"
     gene            complement(1651..2850)
                     /locus_tag="AC16GM_02500"
     CDS             complement(1651..2850)
                     /locus_tag="AC16GM_02500"
                     /inference="COORDINATES: protein motif:HMM:NF017885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR2922576.1"
                     /translation="MPRSPEALIEPSLLIWARKSAGFSTKDVAKKLNVKLEKVEAWES
                     GKARPSVAQLRRLCPIYKRPLAVFFLPEPPRDFMPVRDYRRLADLSSPDEISADLRSE
                     IRRILELRDAAISLLEDIEERKLPELPIVGRYTDNPDELAARIRKALAISSRVQSEWT
                     DEFTALREWRNAAEGLGVLVTQASGIELDEMRGFSVADSQLPLICLNAADRANGRIFT
                     LMHELTHVVLREGGLCEWEHPRRRSPENKQAEAFCNRVAGAVLVPEEDLLQDPTVRGT
                     SGPRAWSDEEVRLLAQRFSVSREVILRRLLIVGRATDTFYQRRREALLKEYKLLGKRK
                     PFPVAYEKRIANALGRSFIQLVLTSYHERRLTLDDTSSLIGVRLKHLPAIEQEVMGWS
                     HIRMAGS"
     gene            3453..3836
                     /locus_tag="AC16GM_02505"
     CDS             3453..3836
                     /locus_tag="AC16GM_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922577.1"
                     /translation="MKRSRDIAWLIRYCKSEPNCYRYLRKKRWRSLGPFCLKCRSPQL
                     WIYPEPSGRNNYICRKCRFHFNDLTASPLHNLRTPLSTYFRLVFLLLLFGEMPIPVLA
                     ALCDVNPKTALRLRNCLWYITPEDE"
     gene            complement(4161..5522)
                     /locus_tag="AC16GM_02510"
     CDS             complement(4161..5522)
                     /locus_tag="AC16GM_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR2922578.1"
                     /translation="MTDQQLRREIFQLAWPIVLSNLLQRGVGIVDTLLVGHVGANELA
                     AVGLCQVLIMVAVALLYAVGTGTNVLVAFHTGANEIKQRREVANTSLWVGFVLSILIS
                     LVGYQLSATGARVMGGTGTVVELAVTYLSITWIFFIFKAYINILSNIFQGTGDTRTPL
                     WVIIGVNVLHVLIAYPLIFGVWGFPRWGVRGAALASGISEALGALVLLAIAIQRKLVA
                     SHFVWYRKEDVRRLVSVGSPVAGERLLIQSMQIVYARLLLGFSVATYAAHQIGLAIES
                     FSFLPGLGFSQAATTLVGQNIGARDFKRASRSGYQSQIIALVFMSILGALFIIFPHFW
                     VSLFTRDPEVIGLGRRLCIILGILQPILAVAMVFSGALRGAGETKFVMYATFTGAWLV
                     RLPFAYLLGIQAGLGLLWVWIAMPVDWAVRALIVWWRYRKVRWASARGGLAAEVERVT
                     AAK"
     gene            complement(5638..6432)
                     /locus_tag="AC16GM_02515"
     CDS             complement(5638..6432)
                     /locus_tag="AC16GM_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922579.1"
                     /translation="MFFSFKQRSLSLKRLFIFLGALSLLLFFGRCSAKKKIFTPALLP
                     VDTPFALVIDAVQATPGTQGVAVEIRNNKSSYDSAIADGGRMGFFNFLVGYDPLVLQL
                     SKVEAGQILKEQRWDFFTYRFGPGNPPCSSCPSSWIRIIAVADINDSVPPTIFDGRNA
                     GGWFTMFFNVSTDSAFCSSFSPIRWIWWSCGDNYVTDSSEKRLWVVDTLRTVTSEIVN
                     VNSTFLIDSSDCNQFYSHWPIRRKFLNFRDGGVRIVCPPVEVAKRR"
     gene            6571..7425
                     /locus_tag="AC16GM_02520"
     CDS             6571..7425
                     /locus_tag="AC16GM_02520"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR2922580.1"
                     /translation="MFVESFITEFKGSRREYCTNPQIIPLRPGDYVIMQAERGEDMGQ
                     FTKRVLFEKAPHGRHSTLEFYPILRKGKREEIERLKHNREREKESLKECKRLVIEYGL
                     QMKVVDVEYQFDSSKITFYFTADRRVDFRALVRELAAIYRTRVELRQIGARDEARRIG
                     GFGRCGLKQCCTTFINRDFPQLTTQLARDQYLPTNPSRITGNCGRLLCCLSYERDTYL
                     AAAELFPRIGAKYQTPKGKGEVVKIDALKMQMAVKLESGEEEVVTALDLRRQKQKEKG
                     LFSLVTAG"
     gene            7429..9363
                     /gene="metG"
                     /locus_tag="AC16GM_02525"
     CDS             7429..9363
                     /gene="metG"
                     /locus_tag="AC16GM_02525"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR2922581.1"
                     /translation="MPNRIFLTTPIYYVNAEPHIGHAYTTIVADLLTRFHQARGDEIF
                     FLTGTDEHGANVAAAAKKAGLSPQEHCDRIAGLYRRTWQALSIKEDYFVRTTDKRHQV
                     AVEKFLLKLHESRTPKGEPIIYEGKYQGLYCLGCEKFLTEKELVNGLCPLHQTKPQLV
                     SEKNYFFRLSAYLDEVQRRIETDQICIRPEERKRETLGLFKQGLEDFSLSREKVTWGI
                     PLPFDPKQTAYVWVDALLNYLSAIGYGDDAKQFDTWWNQAKVVHLMAKEILKFHAIYW
                     PALLLAAGIKPPEFLFIHGFFSVNGQKMSKSLGNVIDPHHLVSRYGPDGTRFLLLTQF
                     PFGVDGDIQAERFTEKYNADLANDFGNLASRVFKLVVDHFNGEVPQPGKPTHEDKDFQ
                     KIALNLAEKTLTAYVGFAGENGRYVYNDALAYTSGLVKEANRYMETQAPWALAKEGKR
                     EQLATVLYYALESLRLAADLYAPALPNKCAELKKALGESQPTGAPFADGPQWGLLKPG
                     TKIKLTSSLFPRLEQEKVKMQTMADDDQKNQVTIDEFGKLDLRVAQVTSAQKVTGADK
                     LLQLEVEVAGEKRQVIAGVAQHYTPEVLLGKKIVVVWNLKPAKIRGLESQGMLLAAQA
                     DGQLVLVTVDGEIKTGAKIS"
     gene            9427..10203
                     /locus_tag="AC16GM_02530"
     CDS             9427..10203
                     /locus_tag="AC16GM_02530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR2922582.1"
                     /translation="MIDTHAHLDFPEFGEDLEAVLARASASGVKNIVTIGIDGESNRK
                     AVALAGQFPQVYAAVGCHPHEADHWSEEFLNDLEKLARHPKVVAIGETGLDFYRNRAT
                     PENQMRAFDAQIDLAIRLKKPLVIHIRNAFERAFTHLVERAEGKTTVVLHCFQEGEEG
                     ARRAVNAGFFLSFNGTLTYKSSALPEVAKALPLESILLETDCPFLPPVPYRGKRNEPA
                     YLSLVLEKLKTVRPARTRAEIDAQIEKNSRAVFGFDSSGL"
     gene            10145..11014
                     /gene="rsmA"
                     /locus_tag="AC16GM_02535"
     CDS             10145..11014
                     /gene="rsmA"
                     /locus_tag="AC16GM_02535"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR2922583.1"
                     /translation="MPKLKRTAERFSASTLVASELTKPHLVPRKSLGQNFLVDRRVQE
                     KIAAALSLKKSDVVVEIGAGRGELTRFLAEQAGAVYAVEIDRRLLPALQSLTKERATV
                     EIIPGDILTVNPREITGDRAVKVAGNIPYHLTSPIIDWLIAQKRRIELAVLLLQREVA
                     RRITGKPETKDWGPLAIAVTCHFHAKKLFDVKPGSFYPPPRVMSSLVEFFTRTEPKVP
                     ARIEEKLLEIVRRAFAHRRKTAINSLILSGGYSAEELVPAFREVNLSPKARGEEITFD
                     QFVRLTEALQKLR"
     gene            11016..>11747
                     /locus_tag="AC16GM_02540"
     CDS             11016..>11747
                     /locus_tag="AC16GM_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922584.1"
                     /translation="MAQLRRLFSILALFWLGGGAFTRASQVQFNFTQGNRTYSLTDSL
                     LLERNFSDHWKLSLTNQSNLLLVKKAFLGGTRDRLQKYATSQIALGYQLSGTAEVGVA
                     LGREITSTDPLGYALSRFSGMLKWTPRNEFSLTTLLGLADQSKDSDLGKKGNSGPHWR
                     SQVRWQKVLTKVGSVHLGASQNFLELKRLPARESNLSAALERKGGLADSLTVELHQRW
                     AKKTYYFDERALSATARQNVNSSFID"
BASE COUNT         2796 a         3427 c         3105 g         2419 t
ORIGIN      
        1 gtgtaggtcg ccgctcccgg cccgcccggc tccgcgcagc cgcccgggat catcaccgcc
       61 gggccttccc gaaacggcca cccgaaattt tcaccaccgt tttcggagag cgatacctcc
      121 tcccaggtat tctgacccac gtcggcaatg aagaggtcgc cggtgggcgg gtcgatattg
      181 aagcggaacg gattgcgcag gccgaaacag tagcagagcc gcgcgttcac attggtggag
      241 gcgacgaaag gattattcga aggcgtgatg ctcgatttcg gcgccgggcc tgcccccgct
      301 cccggcaggg aatcgaccga aagacgcaaa atcatcccgc ggagacccga cgaatcctgg
      361 gccgcgcagg gcaccgcatc ctcgcccaga ctgaggtaca gccgcccatc ggagccaaaa
      421 cgcagggtgc cgccgttgtg gttattggca ttgtccggca tatcggtgag aatgttatac
      481 cggctggctc ccgagatgat gagactgtcg ctcgttggat cgttgaggtc ccccgttacg
      541 gggaacatca cgaggtaatt ggtggccgtc ccggcgtggc tgaagtagca gtagatgtac
      601 ggccgtgccg gccagcccgg atcgaccgca atccccaaaa gcccacgctc gccgccggag
      661 gtattcacgt cggcgatggt gaagagcgga tcccgcgccg cgatggcccc ctgcaccact
      721 aaccggattc ttctggtcac ctgctcgatg aaaaggattc tcccatccgg cagaaacgca
      781 aaccccaccg gctggtttag cccgctcacg atgaccgtat ccacaaatcc atccggcaca
      841 gtggctttaa ccggggatgc gccaaaccag agaaataaaa tcagaatgag caatcccagc
      901 gccgcaccca ccatcttagc cgcttttcct tgaaccgata ctatcttgtt gtagtaagac
      961 tcgctcatac gtggtcgact cttgcgagaa gtatacgagt ttttaggcag gactcaaatt
     1021 ctacttcaca tttagggtta tttttcccct ctccttgagc ggagggatga agggagggtc
     1081 aaagcacaac ccgcgtattt tattcatgta tgcttatcta gacgaaaagc atgtatcccg
     1141 cgcatgtgaa tcgagcgtct atcccacaag cgcctaagga ccggcggccc gaaaactaga
     1201 taacccagtt ttccccctga ataagaccca acaggttgac gcaaggagtc cggaaatgcc
     1261 ggcataccaa gggaatgcgc ggtcgatttg gactcccagg accttcctcg gtaacgacag
     1321 tacaccctcg agctttggct aaggagatca cgaaagggtc agcaatcttt cgcccctcaa
     1381 tacccttggt taacaaagga aactgaacga gaatttccct agctgcccgc tggatatcct
     1441 cttcgagttc aacgaacatt gcttcgcggt ttttgagcca atcagccaga ccgtcttctt
     1501 tcttcttgat ctcgttacgc acttcgatgg gactaatgat tttcccttgg gaaacccatt
     1561 gatcaaatag attccaaaag ttaggtacgt ttattatggg atagcgctcg ttccatgcgg
     1621 caagtaaagc gctcgtgtcg atacagtatg tcatgaaccc gccatcctga tgtgagacca
     1681 acccattact tcctgctcga tagctgggag gtgcttgagc cgcacgccga tgaggctcga
     1741 ggtatcgtca agcgttaggc ggcgctcgtg gtatgacgta agaacgagtt gaatgaacga
     1801 acgcccaagc gcgtttgcga tgcgcttttc ataggcaact ggaaacggct tgcgcttgcc
     1861 gagcaacttg tactctttaa gcaacgcttc gcgcctacgc tgatagaagg tatctgttgc
     1921 gcggcctaca atgaggagcc ggcgaagaat tacctcacgg ctcacagaaa aacgctgggc
     1981 caggagtcgc acttcctcat cggaccatgc ccgtggacct gaggttccac gaacagttgg
     2041 gtcttggagg agatcctctt ccggcaccag aacggctccc gcgactcgat tgcagaatgc
     2101 ctcagcctgc ttgttctcgg ggctccgccg tcgcgggtgt tcccactcac acagaccgcc
     2161 ttcccgtaga acaacgtgag tcagctcgtg cattaaggta aagatgcgac cgttggccct
     2221 atcggccgca ttcagacaga tgagcggaag ctgtgaatcg gccaccgaga agccacgcat
     2281 ctcgtcaagc tctattccag aggcttgagt tacaagtaca ccaagacctt ctgcggcatt
     2341 acgccactcg cggagcgcag tgaactcgtc agtccactcc gactggactc tggaagaaat
     2401 tgcgagggcc tttcggatcc gagcagctag ttcatccggg ttgtctgtat atcgaccgac
     2461 gatcgggagc tcgggtaatt tcctctcttc tatatcctca agaagggaaa tcgctgcgtc
     2521 acgcagttca agtatgcgtc ggatctcgct cctcaaatca gccgagatct catccggaga
     2581 ggacaaatct gcaagccttc ggtaatcacg tacgggcatg aagtcgcgcg ggggttccgg
     2641 aaggaagaaa accgccagag gacgcttata gatcgggcag agacgccgca gctgggcgac
     2701 actgggacgg gctttaccgc tctcccacgc ctcaaccttc tcaagtttga cattgagctt
     2761 cttggcaacg tccttggtcg aaaaccctgc ggacttacgt gcccagatga gaaggctcgg
     2821 ttcgatgaga gcttcggggc ttcgcggcat tctcgctaag ttctcctatg cctgctaacg
     2881 gcctggcgat tagcggtcgg cgctagccag tgcgctgcat cgcctggtta ggcgttggtc
     2941 gcgcttcgaa actggcggca cttcacccat gacttctctt ttgattttca atttatcacg
     3001 aaacataaat caagcaagta ggggtgattt gtgaaccgcc ctttttttat ttctctctat
     3061 ctgaagccct ctttttcgcc cttctaatcc ctgatctctg atccctaatc ccccatttct
     3121 cagaatttgg ggtcactacg ggggtaaaac ggacaaagtg cgaaatagta gagggcctga
     3181 gtgctctgca ccggcctcta cgggactggc ctccttcagg cgggagctcc gctaaagaat
     3241 atgcctcaag cgccagtgca cgccggtcat cactccgctc tcgcaggact acggccccgg
     3301 ggccaccctg gaaaacccag ggcaaaagaa agcgaatcga actccccctc tcctgtagga
     3361 gagggtaggg tgagatctat ctttttccct ctatcctttt ccaaacccgt tgacggtttt
     3421 ctccgtgtcc tccgtgcctc tgtggtggaa ttatgaagcg ttcccgcgat atcgcctggc
     3481 tcatccgcta ctgtaaaagc gaacccaact gctatcgcta tcttcggaaa aaacgctggc
     3541 gctcgctcgg ccccttctgc ctcaagtgcc gcagcccgca actctggatt tatcctgaac
     3601 cttcgggacg aaacaactac atctgccgca agtgccgctt ccacttcaac gacctgaccg
     3661 cctcacccct gcacaacctc cgcacccccc tctccactta tttccggtta gtattccttc
     3721 ttctgctttt cggcgaaatg cccatccctg tcctggccgc cctgtgcgac gttaatccca
     3781 agaccgctct gcgcttgcgc aactgcctct ggtatataac ccctgaggat gagtgaaatt
     3841 ccgtgccgct ccctcgaacc gtgattattc tattgcgcga gaagaccttg ccccgcccga
     3901 ttgcgctctt ttgcgcgaaa aacccgttta cttccccgaa ctgccgcttt tctttgcccg
     3961 ataagatgtt gcgcagtttt cccgccgaac ggcttttccc cgctgttacg tctatctcgt
     4021 tgccgttcaa ccgagacgca gcagaaaaaa tttatgccgt tttgcccccc tcccacgaac
     4081 tctttgcctt taattacgag ttacgaatta ccagttacct gtggttattc tctgtgtcct
     4141 ctgtgcctct gtggtggatt ttatttcgcc gccgtcaccc gctccacctc cgccgccagt
     4201 ccgcctctgg cggacgccca ccttactttc cggtagcgcc accagacgat caacgcccgc
     4261 accgcccagt ccaccggcat cgcaatccag acccacaaaa gccccagtcc ggcctggata
     4321 ccgaggaggt acgcaaaggg cagccgcacg agccaggcgc cggtgaaagt cgcgtacatc
     4381 acaaacttgg tttcccccgc cccgcgcaaa gcaccggaga aaaccatcgc caccgctaaa
     4441 atcggctgga gaatgcccag gatgatgcag agccgccggc ccaggccgat tacttccggg
     4501 tcgcgggtga aaagcgagac ccagaagtgc gggaagatga tgaatagagc gcctaatatc
     4561 gacatgaaga cgagcgcgat gatctgcgac tggtacccgg agcgggaggc gcgcttgaaa
     4621 tcgcgcgccc cgatattttg acccacgaga gtggtcgccg cctgagagaa accgagaccg
     4681 ggcagaaacg agaacgactc tatggccaat ccaatctggt gcgccgcgta agtggcgacc
     4741 gaaaatccca aaagcagccg ggcatagact atttgcatcg attgaatcaa aagccgctcc
     4801 cccgccacgg gagagccgac gctcaccagc cggcgcacat cttccttccg gtaccagacg
     4861 aagtggctgg cgactaattt ccgctggatg gcaattgcca ggagcactag agcaccgagc
     4921 gcttcggaaa tgccgctggc caaagccgcg ccgcgtaccc cccagcgggg aaatccccaa
     4981 acaccgaaga tcaatggata tgcaatcagc acgtggagga cgtttactcc gatgatgacc
     5041 cacaaaggcg tccgcgtgtc gccggtgccc tgaaaaatgt tcgagaggat gttgatgtag
     5101 gctttgaaga tgaaaaaaat ccaagtgatg gaaaggtagg tgaccgccaa ctcgaccacg
     5161 gtgccggtcc cgcccatcac ccgtgcgccg gtggcgctca actggtagcc caccagagaa
     5221 atgaggatcg agaggacaaa cccgacccag agggaggtgt tggctacttc gcgccgttgt
     5281 ttgatttcgt tggccccggt gtggaaggcg accagaacgt tcgtccccgt gcccaccgca
     5341 tagagcaagg ccaccgccac catgatcaaa acctggcaga gccccaccgc ggctaattca
     5401 ttggcgccga cgtgcccgac taaaagcgta tcgacgatac cgacgcctcg ctggagcaga
     5461 ttggagagca caatcggcca ggccagctgg aaaatttccc gccgcagttg ctgatcggtc
     5521 acaagattcg gataatagag ggagttaagc gaaagtcaaa cctgacaaat actaatggta
     5581 gaccggctcg tccctgagcc ggtcagagac tattggagtt ggtattttaa ggtagactca
     5641 acgcctttta gcgacttcaa ccggagggca aacgatgcgt acaccaccat cgcgaaagtt
     5701 aaggaatttg cgccgaatcg gccagtgcga gtaaaattgg ttacaatcgg aactatctat
     5761 aagaaaggta ctgttcacat tgacgatttc actcgtgacg gtacgaagcg tatccactac
     5821 ccagagccgc ttttcactcg aatcagtcac gtagttatca ccgcaagacc accaaatcca
     5881 gcgtattgga gaaaaagaac tacaaaacgc ggaatcagtg ctcacgttaa aaaacatagt
     5941 gaaccaaccg cccgcattac gtccgtcaaa aattgtcggg ggtaccgaat cattaatgtc
     6001 ggcaacagcg attattcgta tccaagaact cggacaggaa ctgcacggag ggttaccggg
     6061 tccgaatcga taagtaaaga aatcccaccg ctgttcttta agaatttgac ccgcctcaac
     6121 ttttgagagt tgtagcacta atggatcata tccgactagg aaattaaaaa agcccatcct
     6181 tcctccgtcg gcaatggcgg agtcatatga actcttatta ttgcgaattt caactgccac
     6241 accctgagta ccgggtgtcg cttgtaccgc atcaattacc aatgcaaacg gggtatcaac
     6301 cggcaaaagt gccggggtaa aaattttctt cttagcagag cagcggccaa aaaagagaag
     6361 gagactgaga gctcccaaaa agatgaataa tcttttcaaa gataaactac gttgcttgaa
     6421 tgaaaaaaac actaaaaaca actcctcttt atgccgggca ctaccctctt ctccgaagat
     6481 ataggaagaa tgtgggctgt caagcaaaat ctaagcaagc cacgccttga cttcccgctt
     6541 cgaaacggtg attatagacg aaaggcgtct atgttcgtcg aatctttcat caccgaattc
     6601 aagggctcgc ggcgggagta ctgcaccaat ccccagatca tacccctccg gccgggggat
     6661 tacgtcatta tgcaggccga gcggggcgaa gatatggggc agtttaccaa gcgcgtcctg
     6721 ttcgaaaaag cgccccacgg ccgccattcg accctggagt tctatcccat tttgcgcaaa
     6781 gggaagcggg aggaaatcga acggctcaaa cacaaccgcg aacgggagaa ggaatcgctc
     6841 aaggagtgca aacgactcgt gatcgagtac ggtctgcaga tgaaggtggt cgatgtggaa
     6901 taccagttcg actcctcgaa gatcaccttc tacttcaccg ccgaccggcg ggtcgatttc
     6961 cgggccctgg tgcgcgaact ggcggcgatc taccggacgc gggtggagtt gcgccaaatc
     7021 ggcgcccgcg acgaggcccg gcggatcggc ggctttggcc gctgcggact caagcagtgc
     7081 tgcaccacgt tcatcaacag ggatttcccc cagctcacca cccagttggc ccgcgaccag
     7141 tacctgccta ccaaccccag ccggatcacc ggcaactgcg gccggctgct ctgctgcctt
     7201 tcgtatgaga gagataccta cttagccgcg gcggaactct tcccgcgcat cggcgccaag
     7261 taccagaccc ccaaagggaa gggcgaagtc gtcaaaatcg acgccctgaa aatgcaaatg
     7321 gcggtcaaac tggagtcggg ggaagaagaa gtggtgaccg cgctcgatct ccggcgccag
     7381 aaacaaaaag aaaaaggcct tttcagtttg gtaacggccg ggtgaggtat gccaaaccgc
     7441 atctttttga ccacgcccat ctactacgtc aacgccgagc cccacatcgg ccacgcctac
     7501 acgaccatcg tggctgacct tttgacccgt ttccaccaag cgcgcgggga tgaaattttc
     7561 ttccttaccg gcaccgacga gcacggcgcc aacgtggccg cagcagccaa aaaagccggc
     7621 ctttcaccgc aggagcactg cgaccggatt gccggtttat accgccggac ctggcaggcc
     7681 ctttccataa aagaagatta cttcgtccgc accaccgata aacggcatca ggtggcagtg
     7741 gaaaaatttt tgctgaagtt gcacgaatcg cggacgccca aaggcgaacc gataatttac
     7801 gaggggaagt accagggtct ctactgcctc gggtgcgaaa aattcttgac cgaaaaagag
     7861 ctggtgaacg gcctctgtcc tctgcaccag accaaaccgc aactcgtttc ggagaagaac
     7921 tatttcttcc gtctttctgc gtatttagac gaagtccaga ggcgaattga aacggatcaa
     7981 atctgtatcc ggcctgaaga acgcaagcgc gagacgctcg gactattcaa acaggggctg
     8041 gaagattttt cgctctcgcg ggagaaggtc acctggggca ttccgctgcc gtttgacccg
     8101 aagcaaaccg cctacgtctg ggtcgatgcg ctcttgaatt acctttccgc catcggctac
     8161 ggcgacgacg ccaaacagtt cgatacctgg tggaaccagg cgaaagtggt tcatctgatg
     8221 gccaaggaga ttttgaagtt ccatgccatc tactggcccg cgctccttct ggccgcgggc
     8281 atcaaacccc cggaatttct tttcattcac ggctttttct ccgtcaacgg gcagaagatg
     8341 agcaagagtt tggggaacgt catcgacccc caccacttgg tctcccgcta cggcccggac
     8401 ggcacgcgtt ttttgctctt gacgcagttc cctttcggcg tggatggcga cattcaggcc
     8461 gaacgcttca ccgaaaagta caacgccgac ttagcgaatg atttcggcaa cctggccagc
     8521 cgggtcttca agttagtggt cgaccacttc aatggagagg ttccgcagcc gggcaaaccg
     8581 acgcatgaag ataaggactt ccaaaaaatt gccctgaact tagcggaaaa aacattgacc
     8641 gcgtatgtcg gctttgcggg cgaaaatggg cgctacgttt acaacgacgc cctcgcctac
     8701 accagcggac tggtaaaaga ggccaaccgc tacatggaga cacaggcccc ctgggcactg
     8761 gccaaagagg gcaagcggga gcaactcgcc acggtacttt actacgcgct ggagagtttg
     8821 cgcctggcgg ccgatctcta cgcaccggcg ctcccaaaca agtgcgcgga gttgaaaaaa
     8881 gcgctgggtg aatcccagcc gaccggcgct cccttcgccg acggaccgca atggggtctc
     8941 ctcaaaccag gaacgaaaat aaaactaacc agttcgcttt tcccgcggct ggaacaggag
     9001 aaagtaaaga tgcaaaccat ggctgatgac gaccagaaga atcaggtgac cattgacgaa
     9061 ttcggcaaac tcgatctgcg ggtggcgcag gtcaccagcg ctcaaaaggt gaccggcgcc
     9121 gataaactgc tccaacttga ggtggaagta gccggtgaaa aacgccaggt catagccggc
     9181 gtggcgcaac actatacacc ggaagtgctc ctgggtaaaa aaattgtggt cgtctggaac
     9241 ttgaaaccgg ccaaaatccg gggcctggag tcgcagggaa tgcttttggc cgcccaggcc
     9301 gatggccaac tggtgctggt gacggtggat ggagaaatca aaaccggtgc gaagattagc
     9361 tgacctcccc cgcacagcat ctcggtacgg acgtcccgcc aaaggcggga cgtttttggt
     9421 tttcgcatga tcgacactca cgcccatctc gatttccccg aatttggcga agatttggaa
     9481 gcggtgcttg cgcgcgcctc cgccagcggt gtgaagaaca ttgtcaccat cggcattgat
     9541 ggcgaatcga accgcaaagc agtagcactg gccggacagt ttccacaagt ctacgctgct
     9601 gtcggctgcc acccccacga agccgaccac tggtctgaag aattcttgaa cgatttggaa
     9661 aaacttgccc ggcacccgaa agtggttgcc atcggtgaga cgggactcga tttctaccgc
     9721 aatcgcgcca cgccggaaaa tcaaatgcgg gcgttcgacg cgcagatcga cctggccatc
     9781 cggctgaaaa aacctctggt cattcacatc cggaatgcat tcgaacgggc ctttacgcac
     9841 ctggtggaac gtgctgaggg gaaaacgacg gtggtgctgc actgttttca agagggagaa
     9901 gaaggtgccc gccgcgccgt gaatgccgga ttttttctct ccttcaacgg cacgctcacc
     9961 tacaagagca gtgcgctgcc cgaagtggcc aaagcgctgc cgctggaatc tatcctcttg
    10021 gaaaccgatt gtccttttct gccacctgtt ccctaccggg gaaaacgcaa tgaaccggct
    10081 tacctgtctt tggtgttaga aaaactaaaa acagttcggc ccgcgcgcac ccgcgcagaa
    10141 atcgatgccc aaattgaaaa gaacagccga gcggttttcg gcttcgactc tagtggcctc
    10201 tgaactgacc aaaccgcatc tcgtccccag gaaaagtctg gggcagaact ttctcgttga
    10261 ccggcgcgtt caggaaaaaa tagccgccgc cctttcgctg aaaaagtccg acgtggtggt
    10321 ggaaatcggc gcgggccggg gtgagttgac ccgttttctg gcggagcaag ccggagcggt
    10381 gtacgcggta gaaattgacc gccgtctgct tcccgcattg caatcgctga caaaggaacg
    10441 ggcaactgta gagattattc caggtgacat tttgacagtg aaccccagag aaatcacagg
    10501 cgaccgggcg gtgaaggtag ccggaaacat cccctaccat ctcaccagtc cgattatcga
    10561 ttggctcatc gcacaaaaac ggcggattga actggcggtt ttgttgttgc aaagggaagt
    10621 ggctcgccgc atcaccggca aaccggaaac taaagattgg ggacctttgg ccattgccgt
    10681 aacctgtcac ttccatgcca aaaaactttt cgacgtcaaa ccgggcagtt tctatccgcc
    10741 gccaagagtg atgtccagtt tagtcgagtt ttttacccga accgagccaa aggtgcccgc
    10801 caggatcgaa gaaaagcttt tggagatcgt ccgccgggcc tttgcccacc ggcgcaagac
    10861 cgccattaat tctttgatct tgagcggagg ctactcagcc gaagaattgg tcccggcgtt
    10921 ccgggaggtg aatctctccc cgaaagcgcg cggcgaagaa ataacgttcg atcagttcgt
    10981 gcgtctgact gaagcactcc aaaaattgag ataaaatggc gcagctccgc agacttttct
    11041 cgattttggc ccttttctgg ctcggcggcg gtgctttcac ccgtgcctcc caggtgcagt
    11101 tcaacttcac ccagggcaac cgcacctaca gcttaaccga ttcacttctg ctggaacgga
    11161 atttctccga ccactggaaa ctttccctca ccaaccagtc gaacctgctc ctggtcaaga
    11221 aggcctttct cggcggcacc cgggatcgcc tccaaaaata cgccaccagc caaatcgccc
    11281 tgggatatca actctccggc acggcggaag tgggcgtggc gctggggcgc gaaatcactt
    11341 ccaccgatcc gctcgggtac gctctttccc gcttttccgg tatgctgaag tggacgccgc
    11401 ggaacgaatt ttctctgacg acccttctcg gcctcgccga tcagagcaag gattccgacc
    11461 tcggcaaaaa aggaaactcg gggccgcact ggcggagcca ggtgcggtgg caaaaggtgc
    11521 tgacgaaagt cggttcggtg cacctcggtg ccagccagaa ttttctggaa ttaaaacgcc
    11581 tgccggctcg cgagagcaac ctctccgccg cgctggagcg caaaggtggt ttggctgact
    11641 ccttaaccgt ggaactccac cagcgctggg cgaagaagac ctactacttc gacgaacgcg
    11701 cgctttccgc caccgccagg caaaacgtta attccagttt cattgat
//
LOCUS       DBNGZD010000094         3199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1891533, whole genome shotgun sequence.
ACCESSION   DBNGZD010000094 DBNGZD010000000
VERSION     DBNGZD010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1891533"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="AC16GM_02545"
     CDS             complement(<1..1785)
                     /locus_tag="AC16GM_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2922585.1"
                     /translation="MKRNSTNKYLRFGLLFIFFCSFRSSPALAQGKQCYDCHTKAKQE
                     YRKKFVHAPVAKGDCESCHQRHGFAQRLILKKEGAELCYSCHADLKDKFSSGTVHPPV
                     PQGACTACHNPHASDEKYLIRRIDNQSACFACHAEVKSQTGLAVQHAPFKAEQCRTCH
                     APHNSATPKLLTAPGDQVCAACHNPADKQLRDKHANFGTDLVGCTDCHSPHASAVPKL
                     LNPKAHQPFALGDCTSCHNVPQKGEPVKLVAPVATVCLTCHGDIEKNLTLPVPHTPAR
                     EKQCDACHQPHTAAQDHLLVEKEAVLCLGCHSDLEKQKKDKSVHVAFAEGKCSACHQP
                     HGSENAKLVKTTANELCLSCHKNISEQMQLAVGHPAVESATCLGCHKPHSSKEPFLLA
                     DEPKAICLTCHESLKPLDKKSVVHPPFSAGDCSACHAPHGAAQAKLLKGEELKVCAAC
                     HPPIYPKAQLKVVHSPFKENNCGACHNPHAADFKNLLVTNEKTVCLSCHGDKASLFSQ
                     KFLHTPVAQNDCSGCHDPHASNLDKFLKVKAEDLCYRCHKTEQKAFAQGVVHQPVAEK
                     QCLTCHNPHGSGVKNGLTAPVPELCATCH"
     gene            complement(1782..2207)
                     /locus_tag="AC16GM_02550"
     CDS             complement(1782..2207)
                     /locus_tag="AC16GM_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922586.1"
                     /translation="MTAKVRLCLLALFIFLASACHSDVSSVTIRVSHLLYPDYHQILT
                     VKMLEKFPLGDTDLSAAVVEFVPDFAIDTVSHKVVSGSQELRNPAFRIVVTQKGEKKE
                     EHWAFFEASVPHFTRQSGLVFETLAFKFNGKTYRREGEK"
     gene            complement(2915..>3199)
                     /locus_tag="AC16GM_02555"
     CDS             complement(2915..>3199)
                     /locus_tag="AC16GM_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2922587.1"
                     /translation="RELRNLVERLFIMTQGASVTAEQVRAVLPELDSAASSVARLSDA
                     VRKFERKQIEAALAAEGGNMTKAALRLGLERSHLYKKLKKLGWQPEELHQ"
BASE COUNT          633 a          873 c          943 g          750 t
ORIGIN      
        1 gtggcaggtg gcgcagagtt cgggtaccgg cgccgttaag ccgttcttca ctccggagcc
       61 gtggggattg tggcaggtga gacactgttt ctccgccacc ggctggtgca ccaccccctg
      121 ggcaaaggct ttttgctctg ttttgtgaca acggtaacac aagtcttcgg ctttgacttt
      181 caaaaacttg tcgagattgc tggcgtgggg atcgtggcag ccggagcagt cgttctgggc
      241 caccggcgtg tgtaggaatt tctggctgaa cagggacgcc ttgtctccat ggcaggacag
      301 acagaccgtt ttctcattgg ttaccaggag gtttttgaaa tctgcggcat ggggattgtg
      361 gcaggctccg cagttgtttt ccttaaaggg agaatgcacg accttcaatt gggccttcgg
      421 gtaaatcgga gggtgacagg cagcgcaaac tttgagttcc tcgcctttga gcaacttcgc
      481 ctgggcggca ccgtgcggag catggcaggc gctgcagtca ccggcgctga acggggggtg
      541 cacaaccgat ttcttgtcta agggtttcaa gctctcgtga caggtgaggc agatggcctt
      601 cggctcatcc gccagtaaaa aaggttcttt ggacgaatgg ggtttgtggc accccagaca
      661 ggtggccgat tctaccgccg gatggcccac ggccaattgc atctgttcgg agatattttt
      721 gtggcaggag aggcagagct cattggcagt ggttttgacc aacttggcat tttccgaccc
      781 gtgcggctga tggcaggcgg agcacttccc ctccgcaaag gccacatgaa ccgacttatc
      841 tttcttctgt ttctccaggt ccgagtgaca gcccaagcac agaacggcct ccttctccac
      901 caggaggtgg tcttgcgccg ctgtatgcgg ttggtggcag gcgtcgcact gcttttcccg
      961 ggctggcgta tgaggaactg gcaatgtcag attcttttca atatccccgt ggcaggtgag
     1021 gcagaccgta gctacaggcg ccaccagttt gaccggttcc cccttctgcg gcacgttgtg
     1081 gcaagatgta cagtccccca gggcgaacgg ttggtgcgct ttgggattca agagcttcgg
     1141 cactgcggag gcgtggggag aatggcagtc ggtgcagccc accaagtcgg tgccaaagtt
     1201 ggcgtgcttg tcccgtagtt gcttgtccgc tggattgtgg caggcggcgc agacctgatc
     1261 tccgggagcg gtcaagagtt tcggggtggc agaattatgc ggggcatgac aggtacggca
     1321 ctgctccgct ttgaaggggg catgctgcac cgccaaaccg gtctggcttt tcacctccgc
     1381 gtgacaggca aagcaggcgg actggttatc aattcttcgg atgaggtact tctcgtccga
     1441 cgcatggggg ttgtgacagg cggtgcacgc gccctgaggg acgggagggt ggaccgtgcc
     1501 ggaagaaaat ttgtccttga ggtcggcatg gcaggagtag cacagctccg ccccctcctt
     1561 tttcaagatc agacgctggg caaaaccgtg gcgctggtgg cagctctcgc agtccccctt
     1621 ggccaccggc gcatggacaa actttttccg gtattcctgc ttcgccttcg tatggcaatc
     1681 gtagcactgc ttgccttgcg ccaaggccgg ggacgaccgg aaagagcaaa agaaaatgaa
     1741 aaggagacca aaccggagat acttgttggt gctgttcctt ttcacttttc gccttcccgt
     1801 cggtacgtct tcccgttgaa tttgaacgcc agagtctcaa aaaccagccc gctctgtcgg
     1861 gtaaaatggg gcaccgatgc ttcaaaaaag gcccagtgct cttctttttt ttcccccttc
     1921 tgggtgacca cgattctgaa agcggggttc cgtaattcct gggaacccga gactacttta
     1981 tggctcaccg tgtcgatggc gaaatccggt acgaattcga ctacggcggc agaaaggtcg
     2041 gtatccccca gtgggaattt ttcaagcatt ttgaccgtga gaatctggtg gtaatccgga
     2101 tacagtaaat gggagacccg gatggtgacg gaagaaacgt ccgaatgaca agcgcttgcc
     2161 aggaaaatga aaagcgctaa aagacaaagg cggactttgg ccgtcaccgg atcatcctat
     2221 ccgcccgccg cacccgtcgc tccggatcgc tttttgcctt ttccggcaca tccgttcacc
     2281 tgctgcgtgc cactagggcc ttcctctcct ttacctatct atacgcttcc caactgctct
     2341 ggtagactat aataaagact gatttgtcct tttatcaagt attatttcac ggccccccca
     2401 aattttttgt ccagtccaga gccccttccc gacggcaccc agcccgatcg ccggctgacc
     2461 gttgacccgg tttggcctgt ttccctgcgc gtgaagccag acgcaaagac gcgagttgcc
     2521 cgcgccgggc ttggatggag tacttgaccg gtctactgat tggggagcga tctgcaagag
     2581 aagtacgccc aaagaaagaa cccgctgctc atgcgggaag tgtagagagg tggtgggaag
     2641 atgtcaagag acgacgcaac gtggtgggca acagcttccg ctccacgggt cagtaaatgt
     2701 tatgcggtga gaacctgacg gttggtgttc ttcttctctc ccttaacctt cccatcctcg
     2761 ccttcagaga cagccgggcg tgggcacacc caggaccacg gacgggacga aaagtacagc
     2821 gatttgtcta tcatcgtttc catcgtggtt gtgaccctgc tgagcaatga ggtttgccga
     2881 cctcaccggt tcagccctac cctcggggac atattcactg gtgcagctct tcgggctgcc
     2941 agcccagttt tttgagtttt ttgtagaggt gactccgttc cagtcccagg cggagagcgg
     3001 ctttggtcat attgccgccc tcagcggcca gggcggcttc gatttgcttt ctttcgaatt
     3061 tccgtaccgc gtcactcaag cgagcgactg aggaagcggc gctgtccagt tccggcagaa
     3121 cagcccgaac ctgctcggcg gtaacgctgg cgccttgagt cataatgaat aagcgctcga
     3181 ccagattgcg caattcccg
//
LOCUS       DBNGZD010000095         6827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_919122, whole genome shotgun sequence.
ACCESSION   DBNGZD010000095 DBNGZD010000000
VERSION     DBNGZD010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_919122"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16GM_02560"
     CDS             <1..353
                     /locus_tag="AC16GM_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2922588.1"
                     /translation="KEMIAKMVAGYETSRLLWLRAAWLKNQGLRNTRETSLAKWFACE
                     EAEAAAADAVQVHGSYGYSDEYPVERFYRNSKGASIYEGTREIHKLIQADYALGIRTD
                     HPVRCALPKWPYEG"
     gene            441..872
                     /locus_tag="AC16GM_02565"
     CDS             441..872
                     /locus_tag="AC16GM_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922589.1"
                     /translation="MKTGRWLVAGIVGGIVYLFFFGLFGGILADKYYDQTPETWRLFS
                     SGWRVKMLVLSLLYGLMVATAYATYAQRMKGPLAQRAVILGLVVWLVSVPQYLMEYVT
                     INVPFPAAFLWIVGGLGGNVGACLAIAYLFGRAPEPAPAQT"
     gene            complement(940..2376)
                     /locus_tag="AC16GM_02570"
     CDS             complement(940..2376)
                     /locus_tag="AC16GM_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR2922590.1"
                     /translation="MKKRLWLKGSLVAAVWFLFIFALVPRGQAAPQPVDPGIRYQFSQ
                     VAEKVLPAVVTVRVEQTVKSQPFAGFPDDQSGDDFFHRFFGTPQGDTQSFRQHGMGSG
                     VIVSSDGFILTNNHVVGRADEISVSVSGRKEPLKAKVIGTDSPTDLAVIKVDAADLPA
                     AKLGNSDSVRIGEWVLAVGAPFQLEHSVTGGIISAKGRSRVGIADYEDFIQTDAAINP
                     GNSGGALVNLVGEVIGINTAIATQTGVYAGVGFAIPINMAKKIMQSLIAEGRVVRGFL
                     GVSIQEVTPEIKEALKTDEGVLVGGVQRGTPADRAGLKPGDVILEFQGKKYTDIDALR
                     SAIAETPPGTKAAFRILRKEGVKTLTVLLAEKPSTQPVALREKPSEEVAESKLGLSAK
                     RITPELAQEMDLSQERGVVITTVKPGSPAAEAGLRAGDVILQINRVAVQALADYQDAL
                     KDLKPNAAVAFLVKRGDTTQFVSMRVPG"
     gene            complement(2542..3819)
                     /locus_tag="AC16GM_02575"
     CDS             complement(2542..3819)
                     /locus_tag="AC16GM_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR2922591.1"
                     /translation="MSHNKRAVKTVAVLSGFLVLFTFLGCGKRRNVVSSSSVAAGALQ
                     LSVHEFPSLKNNPLEAGNPPTDSRARRVIVYTPPGYRPVGFGPPFPVLYLLHQFDGNE
                     NSFGMYQLARVMDELIAAGEIQPMLVVQTDASTIYGGSFYTNSTGTGNYADMIVKNLI
                     DYIERDPIARYNVFGGRRSRAIGGHGMGGYGALRLALENDSLFSSVSCMSAPLAFDGT
                     GGDFKGFIDRLRFVDTMFAENGLLTGGQILVSGRAVVDTAEAFRRYKNLLRPDPGKPL
                     TRLFFAMAAALSPTQQGDKDSLKFKLTLKQADPGMTLPINFLGKVEQPIFDRWVAKDV
                     HTMVKNNPNKLDSLPVYVDAGNQDDFQFNLQSEAFNETVQLLKQAHPFYDYVFEEYAG
                     YPGVPANHTNFISDRLRQVLKFHSDRMLGPPKN"
     gene            complement(3871..5352)
                     /locus_tag="AC16GM_02580"
     CDS             complement(3871..5352)
                     /locus_tag="AC16GM_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR2922592.1"
                     /translation="MWGILFFQKSKLTGNGRCGLRVACAALISLYLLAGAPAWSSGFA
                     LSGVGVKASGMGGAFRAVADDWSAAYWNPAGLAYVTQSEVIVLGQVFSPRPDFTPNVT
                     YNGYEVGYGSVNGTRQFPNDRNFLIGGVSGFYRIPTIEAFTFGVAVFEPHTYASEWNL
                     YKLPYPFKGDSSFAFPEKNFQSKIKVVDIHPTIAKEIIPGRLALGLGIGVEWGTLHLR
                     QPFFQSNPFPPPLNSRPEEFFVVDNRLRTSGWGVGANFGALVKIGSVLRMGASLRSPV
                     TLHLNGDLTSTTYFPFNLAKMQDTLVPASQKFMFGGQSRRFSGAAEGKLNLPWDIGGG
                     VAVFLGKRLTFSADAAYTRWANLWNLQFAVSGQGLLGQPLAPLSMPLHWENTWRGSAG
                     VQVVASDYLTFRGGYHFEGSAIPDQTFSPLFVDLDNKHSVNAGAEAALGGFGFGYSFE
                     WLFASRRNVSNLADVNGDGMYDNLTGTYDFSAFVSYATVSYRF"
     gene            complement(5926..6750)
                     /gene="mutM"
                     /locus_tag="AC16GM_02585"
     CDS             complement(5926..6750)
                     /gene="mutM"
                     /locus_tag="AC16GM_02585"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR2922593.1"
                     /translation="MPELPEVETIARSLRKRMIGRTIITARLFCPKLQRENPPDFSSR
                     VAGSTVQSIQRKGKHLIFLLSDGWAFWVHLMMTGHLFLLQKSHPLVKADRAVFKLSGG
                     LELRFHDIRRFGHLRLVRQQAIGNLKEYRRLGPDALFVSEEEFCSRLRSRKRQMKPAL
                     LDQTLLAGLGNIYADEALFAARIHPLTRTDRLSRPQLRKLWSEIRRLLTRAIHLRGTS
                     VYSYTDTDGRRGKYQEKLKVYGQENAPCPRCRAEVKRLVIGQRSSHFCPRCQKRGA"
BASE COUNT         1460 a         1947 c         1990 g         1430 t
ORIGIN      
        1 tgaaagaaat gatagccaag atggtggcgg ggtatgagac ttcgcgcctc ttgtggctgc
       61 gtgccgcctg gctcaagaac cagggactgc gcaacacgcg ggaaacctcg ctcgccaagt
      121 ggttcgcctg cgaagaggcg gaagcggcgg cggccgacgc ggtgcaggtg cacggctcgt
      181 acggctattc ggacgaatat ccggtggaac gcttctaccg gaactccaag ggcgcctcga
      241 tttacgaagg gacgcgggag attcacaaac tgattcaggc cgactacgcc ctggggataa
      301 ggacggatca cccggtgcgg tgcgcgctgc ccaagtggcc ctatgagggg taatcgtgta
      361 gtacacccgg cagcagaccg atcttcagga cgaggcctgg caagccgtcc agcactcgtt
      421 tagctgcata aggagggagc atgaaaaccg gacgctggct tgtggcggga atcgtcgggg
      481 gaatagtcta cctgttcttc tttggactct tcggcggcat cctggccgat aagtattacg
      541 accagactcc tgaaacctgg cggcttttta gttcggggtg gcgtgtaaag atgttggtat
      601 tgagtctcct ctacggcctt atggtcgcca cagcgtatgc cacctacgcc cagaggatga
      661 aaggcccgct tgcccagcgg gcggtcatct taggattggt cgtctggctc gtcagtgtgc
      721 cgcagtacct gatggaatat gtaaccatca atgttccttt tccggcagct tttctctgga
      781 ttgtgggagg cctcggcggc aacgtgggcg cctgtctcgc catcgcctat ctcttcggac
      841 gtgcgccgga gccggccccg gcgcaaacat aaaaaggccg gggggagacg cgactctcca
      901 ccccggcggg gtaggctgct ctgacccaat gagcatgcgc tacccgggaa cccgcatgga
      961 aacaaactgg gtcgtgtctc cccgcttcac cagaaaggcc acggctgcgt tcggcttgag
     1021 atccttcaac gcatcttggt aatcggctaa tgcctggaca gctacacggt taatctgcag
     1081 gatgacgtcc cccgcacgca agcccgcctc agccgccggg ctgccgggtt tgacggtggt
     1141 aatgactaca cctcgttctt gggaaagatc catttcctgg gccagttcgg gtgtgatccg
     1201 ttttgccgac agacccagtt tcgactcggc cacctcttcc gagggcttct cgcggagagc
     1261 gacgggttgg gtactgggct tttccgccag caacacggtg agcgttttta ctccctcttt
     1321 ccgcagaatc ctaaacgccg ccttggttcc cggcggtgtc tcggctatgg cgctgcgcaa
     1381 ggcatcgatg tcggtgtact tcttcccctg aaattcaaga atgacatctc cgggcttcaa
     1441 acccgcccgg tccgccgggg taccgcgctg gacaccaccg accagtacgc cttcatcggt
     1501 cttcagtgct tctttgatct cgggcgtcac ttcctgaatg ctcaccccca gaaaaccgcg
     1561 caccacccgg ccctcggcaa tcaaactctg catgattttt ttggccatgt tgatggggat
     1621 ggcaaaaccg actcccgcat agacgccggt ctgggtggcg atggcggtgt tgatcccgat
     1681 cacttcaccg actaagttca ccagcgcgcc gccggaattg ccggggttga tggccgcgtc
     1741 ggtctggatg aaatcttcgt agtcggcaat ccccacccgc gagcggccct tggcggaaat
     1801 gatgccgccg gtcacgctgt gctccagctg gaagggggcg ccgaccgcca gcacccattc
     1861 gccgatgcgc accgaatcgg aattgcctaa ttttgctgcc gggagatcag ccgcatcgac
     1921 tttgatgacc gctaagtcgg ttgggctgtc cgttccgata actttggctt tgagcggttc
     1981 cttgcgcccg gagaccgaga ccgaaatttc atctgccctg cccacgacgt ggttattggt
     2041 gaggataaag ccatcggagg agacgatgac acccgacccc atgccgtgtt gccgaaagct
     2101 ctgggtgtcc ccctgggggg tgccgaagaa gcggtgaaaa aagtcgtcgc cggactgatc
     2161 gtccggaaag ccggcaaagg gctggctctt gaccgtttgc tcgactcgga cggtcaccac
     2221 cgccggcagc actttttccg cgacctggga aaactggtag cggattcccg ggtcgaccgg
     2281 ctggggtgcc gcctgcccgc gaggaaccag tgcgaaaatg aaaagaaacc acaccgccgc
     2341 cacaagagaa cctttaagcc ataatctctt cttcatcgta ggtaccttcc tttaaccctt
     2401 tcccacagcc tgacgcttcc agtatttctt atacgtacag agttcccggc cggttgaccc
     2461 tctttcacca cggcgggaag tacggtccga tgaaaaaacc ggggcaggtt gagccccggt
     2521 cggtaggcgg ttgtgcaacg tttagttttt cggcggaccg agcatccgat ccgagtggaa
     2581 cttgagcacc tggcgcaggc ggtcgctgat gaaattcgtg tggttggccg gcacacccgg
     2641 gtagccggca tactcttcaa agacgtagtc gtaaaaagga tgggcctgct tcaagagttg
     2701 caccgtttcg ttgaaggcct cagactgcag gttaaactgg aaatcatcct ggtttccggc
     2761 atcgacgtag accggcaagc tgtccaactt gttgggatta tttttcacca tcgtgtggac
     2821 atccttggct acccaccggt cgaagatcgg ctgttccacc ttgcccaaaa aatttatcgg
     2881 gagggtcatg ccgggatcgg cctgctttaa ggtcagtttg aacttcagac tgtctttatc
     2941 gccttgctgg gtcggcgaca gcgcggcagc catagcgaag aacaaacggg ttagcggctt
     3001 tcccgggtcc ggccgcagga gatttttata gcgacggaat gcttccgccg tatctacgac
     3061 cgctctaccg gagacgagga tttgaccgcc cgtcaacagc ccgttctctg cgaacatcgt
     3121 gtcgacgaac cgcaggcggt cgatgaatcc tttgaaatcg ccgccggtac catcgaacgc
     3181 caaaggcgcg ctcatacagg aaaccgaaga gaagagagaa tcgttttcca gcgccagccg
     3241 cagggcgccg tagccgccca taccgtgccc gccgatggcc cgcgaccgcc gtccgccgaa
     3301 cacgttatag cgggcgatcg ggtcccgttc aatgtaatcg atcaggtttt tgacgatcat
     3361 gtcggcgtag ttgccggtgc cggttgaatt ggtgtagaaa ctccccccgt agatggtgga
     3421 agcgtcggtc tgtaccacga gcatgggttg aatttccccc gctgcaatca gctcatccat
     3481 cacccgggcc agctgataca tgccaaagga gttttcattg ccgtcaaact ggtggagcag
     3541 gtagagcacc ggaaagggcg gtccgaaacc caccggccgg tatcctggcg gggtgtagac
     3601 gatgacccgg cgcgcgcgcg agtcagtggg cggattccca gcttccagtg gattattctt
     3661 gagcgaagga aattcatgca cggagagctg cagggcaccg gccgctaccg acgaggagga
     3721 gaccacgttc cggcgcttgc cgcaacccaa aaaggtaaag agtaccagga aaccgcttag
     3781 aaccgccact gttttcactg cccgtttatt atggctcatg ctgaactccc tttgccgttc
     3841 gcgattttca acttttctgg tcagaagagg ttagaaccgg tagctgacgg tagcgtacga
     3901 aacgaaagcg gaaaaatcgt acgtgccggt caagttatcg tacatcccgt cgccgttcac
     3961 gtcggccaga ttggagacgt tccgccggct ggcgaagagc cactcaaagc tgtagccaaa
     4021 tccgaacccg cccagagctg cttccgcccc tgcgttcaca ctgtgtttgt tgtccagatc
     4081 gacgaaaaga ggcgagaagg tctggtcggg aatcgccgaa ccctcgaagt ggtaccctcc
     4141 ccggaaggtg aggtaatcgg aagccacgac ctggactccg gcgctccccc gccaggtgtt
     4201 ctcccagtgg agcggcatgg agaggggggc caagggctgg cccaggaggc cctgaccgct
     4261 gacggcgaac tgcaggttcc agagattggc ccagcgggtg taggccgcat cggcggagaa
     4321 agtcaaccgt ttgccgagga aaacagcaac cccgccgcca atatcccagg gcaggttcag
     4381 ttttccttcc gcggcgccgg aaaagcggcg tgactggccg ccgaacatga acttctggga
     4441 agccggcacc agcgtatcct gcattttggc gaggttgaag ggaaaatagg tcgtcgaggt
     4501 gaggtcgccg ttcaaatgca gggtcaccgg actacggagg ctggcaccca tgcgaagcac
     4561 gctcccgatt ttcaccaggg caccgaaatt ggcgcccacg ccccagccgc tggtacggag
     4621 gcggttatcc acgacgaaga actcttccgg acgactgttc aaaggcggcg gaaagggatt
     4681 ggattggaag aacggctggc gcaggtggag cgttccccac tcgacgccga ttcccagacc
     4741 caacgccagg cgtcccggga tgatttcttt ggcgatagtc gggtggatgt ccaccacctt
     4801 gatcttgctc tgaaagtttt tctcgggaaa agcgaacgag gaatcgcctt tgaagggata
     4861 cgggagtttg tagaggttcc attcactggc gtaagtatgc ggttcgaaca ccgccacgcc
     4921 aaaggtgaac gcctcaatcg tcggaatgcg gtaaaaaccg gaaacgccgc cgattaaaaa
     4981 attccggtca ttcggaaact gccgggtacc gtttacgctc ccatacccca cctcgtagcc
     5041 attgtaggtg acgttggggg taaaatccgg gcgaggggaa aagacctgcc ccaggacgat
     5101 cacttccgac tgggtgacat acgccaggcc ggcgggattc cagtacgcgg cactccaatc
     5161 gtcggccaca gcgcggaagg cgccacccat gccgctggct ttgaccccga ctcctgataa
     5221 ggcaaatccg gaactccatg ccggtgcgcc agcgagaaga tagagtgaga tgagcgcggc
     5281 gcaggccact ctcaggccgc agcgaccgtt ccccgtcagt ttcgattttt ggaaaaaaag
     5341 aataccccac atccgcttaa ccctcccgaa catttgcctc attttctctt gccacgtaat
     5401 cattgccgaa cccgttagac cccgcactac gaaatagcgc gcaattaaac agatgcccta
     5461 aaagttgtca actctttttt ctggccggtg gacgctcgcc ccggataatc ccattcctgc
     5521 aataattgcc aaagtttaag cgtaaccgct tgccgtttag tttcggaggg gctccgcccg
     5581 ggtgtcgcca ctcacccggg cagagcatgc ggaggcatga agcgttcacg gaggtgagga
     5641 attcaaacgg ttcaaacctc atttctctgg tatggcaacc ggcgtgcccg aatctcgcgg
     5701 aggattatgg tgaggaaaaa ttatccgcta tttcttttgt accaaaaggt tacggcgtct
     5761 gataagacaa gaatgaaggg aaaggtggag tcacttcgta acccaaaaag ggcggaaaat
     5821 cgtcggcgct gacgattctt tacttttgta tttaatcaac cataaaattg acaagaactt
     5881 aatcgatcgg cgaaaatttg ccgccccgcg gacgattttt caagattatg cgcctcgttt
     5941 ctgacacctt ggacagaaat gactggagcg ctgcccgatg accagccgtt tcacttcggc
     6001 ccggcagcgc gggcagggcg cattttcctg accgtaaact ttcagttttt cttgatattt
     6061 gccccggcgt ccgtcggtat ccgtgtaact atagaccgaa gttccccgca ggtgaatcgc
     6121 tctggtcaaa aggcgcctga tttcgctcca gagttttctc agttgcggcc gggaaagccg
     6181 atcggtccgc gtcagcggat gaatgcgggc ggcaaaaaga gcttcgtcgg cgtagatatt
     6241 ccccagcccg gccaaaagcg tttgatcgag aagagcgggc ttcatctgcc gcttgcggct
     6301 gcggagccgg gaacagaatt cttcctccga aacgaagagg gcatcgggac cgaggcggcg
     6361 gtactccttt aaattcccaa ttgcctgctg acgcaccagc cgcagatggc cgaaccgccg
     6421 gatgtcgtgg aaacgcaact ctaagccgcc ggaaagtttg aacacggcgc gatcggcttt
     6481 caccagcgga tgtgattttt gcaaaaggaa gaggtggccg gtcatcatta aatgcaccca
     6541 gaaggcccag ccgtcggata agagaaaaat caggtgcttg cccttgcgct ggatcgattg
     6601 gacggtcgac cctgcgaccc gggatgaaaa atcaggcggg ttttcccgct ggagtttggg
     6661 acagaaaagc cgggcggtga tgatcgtgcg cccgatcatt ctttttctga gactgcgcgc
     6721 gatggtctca acttcgggca actcgggcat gcagattctc tggcagaacg gcgtaattcg
     6781 ctaacacgtt tttcttacgt cttgactttt ttcttttaca gccggtg
//
LOCUS       DBNGZD010000096         3081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_432852, whole genome shotgun sequence.
ACCESSION   DBNGZD010000096 DBNGZD010000000
VERSION     DBNGZD010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_432852"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /gene="tsf"
                     /locus_tag="AC16GM_02590"
     CDS             <1..404
                     /gene="tsf"
                     /locus_tag="AC16GM_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013085.7"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HIR2922594.1"
                     /translation="IHPGDKLGVLVELATPAARGPAQEHIRLLAKDVAMQTAAANPLV
                     VSRAELPPDLTEKEKQIYRSQAVHEGKPEKVLNRIVSGKLEKYFQEVCLLEQPFVKDQ
                     DRMVREVIAETEQKVGEKITVRRFARFRLGE"
     gene            473..1195
                     /gene="pyrH"
                     /locus_tag="AC16GM_02595"
     CDS             473..1195
                     /gene="pyrH"
                     /locus_tag="AC16GM_02595"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR2922595.1"
                     /translation="MPQTKYRRILLKISGEALMGAKEFGIDPDVIVSVAEQIKEVHEL
                     GVQIGVVVGGGNIFRGLAAGEAGIERVTGDQMGMLGTVINALALQDRLEKLGVYTRVM
                     SAIRMEAVAEPYIRRRAVRHLEKGRVIILAAGTGHPYFTTDTAAALRATEVEAEVILK
                     ATKVDGVYSADPKTDKNAKLFDTLSYLDVLSLGLKVLDATATSLCMDNQLPIIVFNLS
                     QRGNLKKIVLGDRIGTIVQNTR"
     gene            1228..1785
                     /gene="frr"
                     /locus_tag="AC16GM_02600"
     CDS             1228..1785
                     /gene="frr"
                     /locus_tag="AC16GM_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR2922596.1"
                     /translation="MIKQIYAETEEKMKKAVEVLSRELASTRTGKATTALLDGIRVDY
                     YGSHVPLNQVASLSVPEHRLLTIQPWERNLIPEITKAIQKSELGLTPSTDGNVIRLPI
                     PPLTEERRRDLVKHVKKLAEESKIALRNIRRDANEHLKKAKTDKKISEDDEKHAEKHV
                     QELIDKYIEAVDKLIKQKEAEIMEV"
     gene            2026..2358
                     /locus_tag="AC16GM_02605"
     CDS             2026..2358
                     /locus_tag="AC16GM_02605"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR2922597.1"
                     /translation="MRQRGAFRLLAFVIMPDLLHLLLQPVNGKSLPNIMHSLKSYTAK
                     KLMRVLPGLRKVWQDGYYEHSIRTERELVEKIRYVESNPLKSGLCRDPEKFCFSSASK
                     REQMDTRT"
     gene            2454..>3081
                     /gene="uppS"
                     /locus_tag="AC16GM_02610"
     CDS             2454..>3081
                     /gene="uppS"
                     /locus_tag="AC16GM_02610"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR2922598.1"
                     /translation="MSKERLRTEILARGNLPRHVAIIMDGNGRWAKKRGLPRTAGHKA
                     GVEAVRRTVRAAGELGIPYLTLFAFSVENWRRPREEVAAIMKLLYETTRRELRELMEN
                     NVRLIATGRLSELSPLRRAALRRAIEKTGANTGLTLNLALNYGGRTEILDAVRKACRE
                     ARAGRLNPEKLDEKLFANYLYTAGLPDPDLLIRTSGELRLSNFLLWQTS"
BASE COUNT          753 a          890 c          878 g          560 t
ORIGIN      
        1 acatccaccc cggagacaag ctcggcgtcc tggtggaact ggcgactccg gcggctcggg
       61 gtccagcgca agagcacatt cgcctcctgg ccaaagacgt cgccatgcag actgccgcgg
      121 ccaatcccct ggtggtcagc cgggccgaat taccgcccga cctgactgaa aaagaaaaac
      181 agatttaccg ctcccaggcg gtgcatgagg gcaaaccgga aaaggtgctc aatcgcatcg
      241 tgagcgggaa attagaaaag tactttcagg aagtctgcct tttggaacag ccgttcgtta
      301 aggaccagga ccggatggtg cgggaggtca ttgccgaaac cgagcaaaaa gtgggggaga
      361 aaatcaccgt ccggcgcttc gcccgcttcc gcctgggaga gtgacgcgcg ggagccggac
      421 gtattcgcac gggcagggaa tgccggtaac aatccgagag aagactgaag cgatgcccca
      481 gaccaagtac cgcaggattc tgttgaaaat ctctggcgaa gccttgatgg gggcgaaaga
      541 attcggcatc gatccggacg tgatcgtctc ggtagccgaa caaatcaaag aagttcatga
      601 gctgggggtg caaatcggcg tggtcgtggg aggcgggaac atcttccgcg gcctggccgc
      661 cggcgaggcc ggcattgagc gcgttaccgg cgaccagatg gggatgctgg gtactgtcat
      721 caacgccttg gccctgcagg accggctgga gaaattagga gtctacaccc gggtgatgag
      781 cgccatccgg atggaagcgg tggcggagcc ctacatccgc cggcgcgcgg tgcgccacct
      841 cgaaaaaggc cgggtcatca tcctggctgc cgggaccggc cacccctact tcaccaccga
      901 cacggcggcc gccctgcggg ccaccgaagt cgaggccgaa gtgatcctga aagccaccaa
      961 agtcgacggc gtctactccg ccgatcccaa aaccgacaag aacgccaaac tgtttgacac
     1021 cctgtcgtac ttagatgtct taagcctggg actcaaagtg ctggatgcca ccgccacctc
     1081 gctctgcatg gacaaccagc tccccattat cgtcttcaac ctgagccagc gcggaaattt
     1141 gaagaaaata gtcctgggcg accgcatcgg taccatcgtg cagaataccc gctgaaggca
     1201 tccggaaggg agggaaccga gcgtccgatg atcaagcaga tttacgctga aaccgaagag
     1261 aagatgaaaa aagcggtgga ggtgctctcc cgcgaactgg cttcgacccg caccggtaag
     1321 gcgacgaccg cactcctgga cggcatccgg gtggactact acgggagcca cgtgccgctg
     1381 aaccaggtgg cctccttaag cgtgccggag caccggcttt tgaccattca accctgggaa
     1441 aggaatttaa ttcccgaaat caccaaagcc attcagaaat cggagctggg gttaacaccc
     1501 agcaccgacg gcaatgtcat ccggctgccg atccccccgt tgaccgaaga gcgccggcgc
     1561 gatttggtca agcacgtaaa gaaactggcg gaggagagca aaatcgctct gcgcaacatc
     1621 cgccgggacg ccaatgagca tctgaagaaa gcgaaaacgg acaagaaaat ttccgaagac
     1681 gacgagaagc acgccgagaa gcacgtccag gagttgatcg acaagtacat cgaagcggtg
     1741 gacaaattga tcaagcagaa ggaagccgaa attatggaag tctaacccga gtttttttgc
     1801 gaagcgtgcc tcacccctct cgttcgggag gggtttttgt tacaatggtc tcaaatattc
     1861 tgggtaggcc gggcatcctg cccagccaaa gcggggtcat ttgagcagac caagacacat
     1921 cgccgtcgcg aacgcagtgt acttagttac gacggtaacc cagaaccgga atcctctctt
     1981 ctctgaaccg gcagcagccc aactcgttca aaacgccttt ttttcctgcg tcagcgcggc
     2041 gcctttcggc tcttggcctt tgtcattatg cccgacctcc tccatctcct cttacaaccg
     2101 gtgaatggaa aaagtctgcc caatattatg cacagcttaa agagttatac cgccaagaaa
     2161 ttgatgaggg ttctaccggg tttgcggaaa gtctggcagg atggatatta cgaacacagc
     2221 attcgcaccg agcgtgaatt ggttgaaaaa attcgttatg tcgaatccaa cccccttaaa
     2281 tccgggctat gtcgcgaccc ggagaaattc tgcttttcat cggcgtcaaa acgagagcaa
     2341 atggatacgc ggacataaaa accaaaaatg gcaccgggca ggatgcccgg cctacccgtc
     2401 ccaagaaata gcacctgctt tttcaaagac atttctgtta tactgacttt tcgatgtcaa
     2461 aagaacgtct gagaaccgaa atcctggccc gagggaatct gccccgccac gttgccataa
     2521 ttatggacgg caacggccgc tgggcgaaga aacgcgggct gccccgtacc gccggacaca
     2581 aagccggcgt ggaagcggtg cgccgcaccg tccgggctgc cggtgaactg ggtatcccct
     2641 acctcaccct cttcgccttt tcggtggaaa actggcgccg gccgcgggaa gaagtggcgg
     2701 cgattatgaa actcctgtac gagactaccc ggcgcgaact gcgggagttg atggaaaaca
     2761 acgtacggtt gattgccacg ggtcgcctga gtgaactctc ccccttgcgc cgtgccgccc
     2821 tgcgccgagc cattgagaaa accggagcaa ataccggctt gacgctcaac ctggcgctca
     2881 attacggggg ccggacggaa atcctggacg cggtgcggaa agcctgccgg gaggcccggg
     2941 ccggtcgtct caatccggaa aaattggacg aaaaactttt tgcgaattac ctctacaccg
     3001 ccggactgcc cgatcccgat ttgctcatcc gcacgtccgg cgagctgcgc ctgtcgaact
     3061 tccttctctg gcaaacttct t
//
LOCUS       DBNGZD010000097         5943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1405453, whole genome shotgun sequence.
ACCESSION   DBNGZD010000097 DBNGZD010000000
VERSION     DBNGZD010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1405453"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="AC16GM_02615"
     CDS             <1..628
                     /locus_tag="AC16GM_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2922599.1"
                     /translation="IREIQQQTGEISPPPGFHIGATGQAEAQKESFGYIFQALFLAVV
                     FVYLLLASQFESFVDPFAIMLSLPLSLVGAFLGLFLTGSSVSIMSLIGVVMLMGLVTK
                     NAILLVDFTKQLRRKGVERTEALLTAGPIRLRPILMTTFAMVFGMAPLALGFGPGAEM
                     RAPMARAVIGGVISSTLLTLVVVPVVYTMLDDLVHRKRKTAPLPVTES"
     gene            783..2420
                     /gene="bshC"
                     /locus_tag="AC16GM_02620"
     CDS             783..2420
                     /gene="bshC"
                     /locus_tag="AC16GM_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HIR2922600.1"
                     /translation="MQKTIDFRQIAATTRLFADFLYAPDKVQEFYNGSSADLESFRRV
                     GEFLRSRNYPRSAIAGILKEQNQAFGAGARTLANLDLFQTPDSRAVFAGQQVGLFGGP
                     LFTLYKMITIIKWAEKLGAQLSQKCIPFFWLATDDHDFAEIDHIAVLDHKNQLVQIRY
                     APGTPHNGSSMARVVLEATLEATIDELSRHLPETEFKSEVIAALRDCYQAGQPLHLAF
                     ARWVFRLIGDRGLVLVNPADPRLKALAQPVFQREIESTVQTSRLTQQTSSRLIQKQYH
                     AQVHKAETIVNLFYHDGNRAGISRSGDRFAIDGEKRSFSSAELVREVAVHPERFSPNV
                     FLRPVVQSAVFPTVAVVTGPSEVAYFAQIKGLFEHHGVPMPVLCPRVSLTVVEKRIAT
                     LVEKYQIDLGAFFRDPEGTTNRVLHQAIADPLEPKIDRTRTDLQEKLSTLEPDLVTLD
                     PTLKATFGQIKARVDLELKNLEKKVFAAQKKKNETIVEQLQRLRNHLYPGNKLQERQF
                     NIVYFLVKYGFGFSNHLFDALDIDRRDHQLVEVGGEG"
     gene            2417..3538
                     /gene="bshA"
                     /locus_tag="AC16GM_02625"
     CDS             2417..3538
                     /gene="bshA"
                     /locus_tag="AC16GM_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HIR2922601.1"
                     /translation="MKIGIACYPVPGGSGAVASELGLALARRGHEVHFLSYALPFRLA
                     AYTENFFFHEVEVSDYPLFKYPPYTLTLASKMIEVSRQWGLDVLHVHYAIPHASAAFL
                     AKQVLQSRLPVVITTLHGTDITLVGTNKSFYDVTKFSIECSDGITAVSDFLRQKTVAE
                     FDIKKEIRVIPNFVDTERFSDKNPACQRARFAPNGEKILMHLSNFRAVKRIGDVIEVF
                     RRVRKEIPAKLVLIGEGPETGQALALTRKFKLQNDTIFLGQQEFVENLLCLGDLFLLP
                     SETESFGLAALEALSCGVPVLATRTGGLAELVRDGENGFLFPVGESGKMAEQALELFR
                     DGDQLAWMKRQARQSAVERFDAKKMVPLYEAYYREVAGA"
     gene            3664..4374
                     /gene="bshB1"
                     /locus_tag="AC16GM_02630"
     CDS             3664..4374
                     /gene="bshB1"
                     /locus_tag="AC16GM_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HIR2922602.1"
                     /translation="MNLDCLVFAAHRDDIEITCGGTVIKMTDLGYKVGICDLTQGEMG
                     TKGTEVDRAREADAAAKVMGLSVRENLKLPDAGVQNTRENQLKVVEILRRYKPHLLIL
                     PYWEQRHPDHAVTPRLVYDACFLSGLKKLGAPGEPHRPFKILYSTSFFEIKHSFIVDI
                     TNQFSRKLEAVRCYNSQFDDTPESKEIFPPARNIFEFMETKARHYGYLVGKKYGEAFL
                     TREYVEVEDPMKLLVKSI"
     gene            4453..5160
                     /locus_tag="AC16GM_02635"
     CDS             4453..5160
                     /locus_tag="AC16GM_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HIR2922603.1"
                     /translation="MNRGNNNSTSFLNELDAIIAQRHMLSHPFYQAWSAGELSLETLR
                     HYARQYYHHVITFPRYLSNVHANCEDGQTRQVILENLIEEERGPENHPELWLRFGEAL
                     GDTREQVQSAIPLPETEAAVGTFLRLTREGSVAEGLAALYAYESQIPEVSKTKTEGLK
                     KFYGVTNLRGTAYFTVHEEADVRHAAQERELLASLSNTDITRRKARTSVRLALEAAWQ
                     LLDGIARTAPQPIVPCR"
     gene            5167..5358
                     /locus_tag="AC16GM_02640"
     CDS             5167..5358
                     /locus_tag="AC16GM_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922604.1"
                     /translation="MRPSEAEVKYLEVLRGFSGEERVRIGFELYRLARRIVEASVRAQ
                     YPDASDEEVERHIRQRLAA"
     gene            5355..5912
                     /locus_tag="AC16GM_02645"
     CDS             5355..5912
                     /locus_tag="AC16GM_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922605.1"
                     /translation="MTLEEVLVFVLRRLHDQNIPYMLTGAVAVNYYGRPRLTHDIDLV
                     VEVREKQIPALVTAFAQDFYASQEGITDAIVHRSSFNFIHHASGLKIDFWLVQGTPFD
                     EERFARRQQVVLFDERVWVTTAEDLILIKLLWLKESGSQKHRDDIQGIVAVQKDRLSN
                     QYVERWTVQQSTRALWGDVIAHLES"
BASE COUNT         1290 a         1772 c         1616 g         1265 t
ORIGIN      
        1 gatccgcgag atccagcaac agaccggaga gatcagtccc ccgcccggct tccacatcgg
       61 cgccaccggc caggccgagg cgcaaaaaga atcgttcggc tacatctttc aggcgctctt
      121 tctggcggtc gtcttcgtct acttgctttt ggcttcacaa ttcgaaagtt tcgtcgatcc
      181 tttcgccatc atgctctcgc tcccgctttc cctggtgggg gcgtttctgg gccttttcct
      241 caccggcagc tcggtctcga ttatgtcgct catcggcgtc gtgatgctca tgggtctggt
      301 gaccaaaaac gccatcctct tggtcgattt caccaagcag ctccggcgca agggggtgga
      361 gcgtaccgaa gcgcttttga ccgccgggcc gatccggctg cggccgattc tcatgaccac
      421 gttcgcgatg gtgttcggca tggccccgct ggcgctgggg ttcgggcccg gagcggaaat
      481 gcgggcgccg atggcgcggg ccgtcatcgg cggggtaatc tcttcgaccc tcctcacctt
      541 agtcgtcgtt ccggtcgttt acaccatgct ggatgacctg gttcaccgga agcgcaagac
      601 cgctccgctt ccggtgaccg aaagctgatc tctcttctcg cgcagttggg agcatcccca
      661 aaagtgctca atgcggtggc gccggttggt ttttgaagcc ggcactacag attttccttt
      721 accggaaatg ttattcccca ccccctgaat agcgtatatt gtcaaagatt tcgagggagt
      781 gaatgcagaa aaccatcgat tttcgccaga ttgcggcgac gaccagactc ttcgccgatt
      841 tcctctacgc cccggacaag gtccaggagt tctacaacgg aagttccgct gaccttgaaa
      901 gtttccggcg ggtaggcgag tttctgcgtt cgcgaaatta cccgcgttcc gctatcgccg
      961 gtattcttaa ggaacaaaac caagccttcg gtgccggagc caggactctg gctaacctcg
     1021 atcttttcca aacgcccgac agccgggcgg tctttgccgg tcagcaggtg ggcctcttcg
     1081 gcgggccgct cttcacactc tataagatga tcacgatcat caagtgggcg gaaaagttag
     1141 gcgctcaact gagccagaag tgtattccct tcttctggct ggccaccgac gaccacgact
     1201 ttgccgaaat tgaccacatc gccgtcctcg atcacaagaa tcagttagtc caaatccgct
     1261 acgcaccggg cacaccccat aatggctcgt caatggcgcg agtggtgctc gaggcgaccc
     1321 tcgaggcgac catcgatgaa ctttcccgac acctacccga aacagaattc aaaagtgaag
     1381 tgatcgctgc cctgcgcgac tgctaccaag cgggtcagcc gctccacctc gcctttgccc
     1441 gttgggtctt tcgcttgatt ggtgaccggg gattggtcct ggtcaatccg gccgatcccc
     1501 gattgaaggc gctggcgcag ccggtctttc agcgtgaaat cgaatcaacc gtccaaacca
     1561 gtcggcttac ccagcaaaca tccagccggc tcattcaaaa gcagtaccat gcgcaggtgc
     1621 ataaggccga gacaattgtc aatctcttct accatgacgg caaccgggcc ggcatcagcc
     1681 gctccggtga tcggttcgcc atcgacgggg aaaagcgctc cttttcatcc gccgaattgg
     1741 tgcgggaagt ggcggtccac ccggagcggt tcagcccgaa cgttttcttg cggcccgtgg
     1801 tgcagagtgc ggttttcccg acggtggccg tggtcaccgg cccgtcggag gtggcctact
     1861 tcgcgcagat caaggggctc tttgaacacc acggtgtccc gatgccggtc ctctgtcccc
     1921 gagtctcgct gacagttgtt gagaagcgca ttgccaccct ggtagagaaa taccagatcg
     1981 atctgggagc cttcttccgc gatcctgagg gcaccaccaa ccgcgtcctc caccaggcca
     2041 ttgccgatcc cttagagccc aaaattgacc ggacgcgcac cgatttacag gagaaactct
     2101 caactctgga acccgacctg gtgacgctcg atccgacgct gaaagcgacc ttcggtcaaa
     2161 ttaaagcccg agtggacttg gaactgaaaa acttagagaa gaaggttttt gccgcgcaga
     2221 agaaaaagaa tgaaaccatc gttgaacaac tccagcgatt gcggaaccac ctctatcccg
     2281 gaaacaaatt gcaggagcgg cagttcaata tcgtctactt cttggtgaag tacggtttcg
     2341 gtttcagcaa ccacctcttt gacgcgctgg atatcgaccg gcgcgaccac cagctcgtcg
     2401 aagtcggagg cgagggatga aaatcggtat cgcctgttac cccgtcccgg gcggttccgg
     2461 cgcagtggcg agtgaattag gcctggcctt ggcccggcgg ggacacgagg ttcactttct
     2521 cagttatgcg ctgcccttcc gtctggctgc ctataccgag aacttcttct ttcatgaggt
     2581 agaagtcagc gattacccgc tcttcaaata cccgccctac acgctcaccc tggcttccaa
     2641 gatgatcgag gtctcgcgcc agtgggggtt ggatgtcctc cacgtccact acgccattcc
     2701 ccacgcttca gccgcattcc tggccaagca ggtgctccag agccgcctgc cggtggtcat
     2761 caccaccctg cacggcaccg acattacgct cgtcgggaca aacaagtcct tctacgacgt
     2821 caccaagttt tccatcgagt gttccgacgg catcactgct gtctccgatt tcctgcggca
     2881 gaaaaccgta gccgaattcg acatcaaaaa agaaatccgg gtgattccca acttcgtcga
     2941 tactgagcgc ttctcggata aaaacccggc ctgccagcgc gcccgtttcg cccccaacgg
     3001 ggaaaagatt ctcatgcacc tctcgaattt ccgagcggtc aagcggattg gggacgtaat
     3061 cgaggtcttt cgccgggtac ggaaagaaat tccagccaaa ttggtgttga tcggcgaagg
     3121 ccccgagacg ggccaggcgc tcgccctgac ccgaaaattc aaactgcaga acgacaccat
     3181 ctttttgggc cagcaggaat ttgtggaaaa tctcctgtgt ttgggcgacc tgttccttct
     3241 gcccagcgaa accgagagtt tcggactggc cgcgctggaa gccctgagct gcggcgtgcc
     3301 ggtgctggcg acccggaccg gcgggttggc ggaactcgtt agagacggtg aaaacgggtt
     3361 cctcttcccg gtgggagaga gcgggaagat ggctgagcag gcgctggagc tgttccggga
     3421 tggcgaccaa ctggcctgga tgaaaagaca ggcgcgccag agcgcggtcg agcgctttga
     3481 cgccaagaaa atggtacccc tgtacgaagc ctactaccgg gaagtggcgg gagcataaga
     3541 tcagtttttt acggctggcc caactccgca acccacttgc aaaattccac aaatcaagca
     3601 tcttaccccg tttcgtggag gtgctcgttc aggacctgtg acgaaaaata gtgtggagat
     3661 ggaatgaatc ttgactgtct agtctttgcc gctcaccggg acgacatcga aatcacttgc
     3721 ggtggcacgg tcattaaaat gactgacctg gggtacaaag tcggcatctg cgacctgacc
     3781 cagggcgaga tgggcaccaa agggactgaa gtcgaccgtg cccgcgaagc cgatgccgcc
     3841 gctaaagtca tggggctttc ggtccgcgaa aatctgaaac tccccgatgc cggcgtccag
     3901 aacacccgcg agaatcaatt gaaagtggtg gagatactcc gccggtataa accccatttg
     3961 ctcatcctcc cctactggga gcagcgccac cccgaccacg ccgtcacacc ccgcttggtc
     4021 tacgatgcct gcttcctctc cggtctgaag aaattgggag ctccgggcga gccccaccgg
     4081 cccttcaaaa tcctctacag cacctcgttt ttcgaaatca agcactcctt catcgtcgat
     4141 attaccaacc agttttcgcg gaaattagag gcggtgcgct gctacaactc ccagttcgac
     4201 gacactcctg aatcgaaaga aatctttccc cccgcccgca acatctttga gttcatggaa
     4261 accaaggccc gccactacgg ctacctcgtc ggcaaaaagt acggcgaggc gttcctgacc
     4321 cgggagtacg tggaggtcga agatccgatg aagttgctag tgaagtcaat ttaagatttc
     4381 cgtttttcca gcccattgtg tatactccgg actaattcat cggtccgaaa ggtgataacg
     4441 aaaggagtca cagtgaatcg gggaaataat aattcgacct catttctcaa tgaactggat
     4501 gccattatcg cccaaaggca tatgctttcc caccccttct accaggcctg gagcgcgggg
     4561 gaactctcgc tggaaacgtt gcgccactac gcccggcagt actaccacca tgtcataacc
     4621 tttccccgct acctctccaa cgtccacgcc aactgcgagg acggtcagac tcgccaggtg
     4681 atcctggaga atttgatcga agaggaacgc ggcccggaga atcaccccga actctggctg
     4741 cgcttcggcg aggcccttgg cgacacccgg gaacaagtcc aatctgcaat tcccttgccg
     4801 gaaacagagg cggcggtcgg gacttttctc cggctcaccc gcgagggctc cgtggcggaa
     4861 gggctggccg cgctctacgc ctacgagtcg caaatccctg aagtttccaa aaccaagact
     4921 gagggattaa agaaattcta cggcgtcact aacctccggg gtactgccta cttcacggtt
     4981 cacgaggaag ccgatgtgcg ccacgccgcg caggaacggg agttgctcgc ctcactttcc
     5041 aacacggata taacccggcg aaaagcacgc accagcgtcc gactggctct ggaagccgcc
     5101 tggcagcttc tggatggaat cgctcgaacc gcacctcagc cgattgtgcc ttgccggtga
     5161 gtacccgtgc gaccgtcgga ggcagaagtc aaatatctcg aagtactccg ggggttttcc
     5221 ggtgaagagc gggtgcgtat tggttttgag ctctaccgct tagcccgccg gattgtagaa
     5281 gcttcggtac gagcccagta tccggacgcg tccgatgaag aggttgagcg tcacatccgt
     5341 cagcggttgg ccgcatgacc ctggaggaag tgctcgtttt tgtcctacgc cgtctgcacg
     5401 accaaaatat accctacatg ctcaccggtg cggtggcggt gaactactat ggtcggccgc
     5461 gcttgaccca cgacattgat ctggtggtag aagttagaga aaagcaaatc ccggccctcg
     5521 ttaccgcttt cgcccaagat ttttatgcca gccaggaagg gattaccgat gccatagtgc
     5581 accggtcatc attcaacttc attcaccatg ccagtggttt gaaaatagat ttctggctgg
     5641 tgcaagggac tccctttgac gaagagcgtt ttgcgcgccg ccagcaggta gtgctttttg
     5701 atgaaagggt ttgggtgacc actgctgaag acctcatact cattaagctc ctgtggttga
     5761 aagagtcagg atcccagaag caccgcgacg acatccaagg aatcgtagca gtacagaaag
     5821 atcgcttgag caaccagtac gttgagcgct ggactgttca gcagagcacc cgggcactct
     5881 ggggcgatgt gattgcccat ttagagagct aatcgcccaa agtcgatttc gcctccgctt
     5941 gcc
//
LOCUS       DBNGZD010000098         2781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1614063, whole genome shotgun sequence.
ACCESSION   DBNGZD010000098 DBNGZD010000000
VERSION     DBNGZD010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1614063"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="AC16GM_02650"
     CDS             <1..205
                     /locus_tag="AC16GM_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922606.1"
                     /translation="VQAVGVSIDVFLRDALGQRRLPCADQGGHVGAKIPQGDVPFIYL
                     ASPATCAASAIEGKIADPRRYLS"
     gene            202..714
                     /locus_tag="AC16GM_02655"
     CDS             202..714
                     /locus_tag="AC16GM_02655"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR2922607.1"
                     /translation="MTDISLRMRGRVWLFGNDVSTDLIFPGRYVHLLSNLPELTKHTL
                     EDARADYAAGVKPGDFVVAGENFGMGSSREQAALIIKMSGASAVIARSFARIFFRNSI
                     NNGLPLIETDTTGIDDGDILEVELNAGKLSNVTRGWERQFNPLPKIMTDILADGGLVA
                     HIKKHGDFNL"
     gene            746..1576
                     /locus_tag="AC16GM_02660"
     CDS             746..1576
                     /locus_tag="AC16GM_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922608.1"
                     /translation="MEKTDVLSYIKGCSQEIATRFDELFSGEMESFATEMTSTFSILQ
                     KARNYTKQPGASEIDHNSVMILWSKANSLISAFELVRRGYIVEPLILARHALEGAAVV
                     VDILKSPQKLSKFFSQKYKSTGAISVAKEVIPIFGKIYGGLSDNLVHIGLLSCMPQWG
                     MGPNFGIQSLFLGGGIDENRIEDYVIVLCSLDLCTDVFNTIVEYSLFKFVDSPRFWRK
                     KSETTLSFEPDDVIKDRHEERQRKLVEAFQKLMGRVEKQQEILSRIDGGNDSNTLLPG
                     "
     gene            1650..2060
                     /locus_tag="AC16GM_02665"
     CDS             1650..2060
                     /locus_tag="AC16GM_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922609.1"
                     /translation="MKLRQRLILIFGFILIMLRFLFPPVEISLADRVEEEKRTKELMA
                     NFDDLIKGMTELSGGEITPPMQDLINAHQELVNKRGRFMATYSSRPFHFLFNTPPRPA
                     KFVWNVVIIHVLIITILAWFGYWILKRSPRSNPR"
     gene            2272..>2781
                     /locus_tag="AC16GM_02670"
     CDS             2272..>2781
                     /locus_tag="AC16GM_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922610.1"
                     /translation="MKIKTKFWSAAVGSLPLMLGVYLPDSTVHSLKVAGGTGQYATIF
                     RDCAGNPTSSHGIPYKDVGGEYSFSPPGAASLGLRASRVWDRTVTGSFPDTFLLRRER
                     TIINPFFQLDWRKFGIGGGVAFNGRQNKVTGEKSAMPSWYLRIARGPIYFSSTFFCGV
                     PLYSGGGGLN"
BASE COUNT          695 a          659 c          706 g          721 t
ORIGIN      
        1 ggttcaggcc gttggcgtaa gcatcgacgt attcttgcgt gatgcgctgg gccagcgccg
       61 tctgccgtgc gccgatcagg gcggccacgt gggggcgaaa atcccgcaag gagatgtgcc
      121 cttcatctat ttagccagcc cggccacctg cgcggcttcg gccatcgaag gaaaaatcgc
      181 cgacccccgg aggtatctgt catgaccgat atttcgttaa gaatgcgggg acgcgtctgg
      241 cttttcggca acgacgtttc caccgacctc atcttccccg gccgctacgt ccaccttctc
      301 tccaacctgc cggaattgac caaacacacc ttagaagacg cccgggccga ctacgcggct
      361 ggcgtcaagc ccggcgactt tgtagtggcg ggcgaaaatt ttgggatggg ctcctcccgc
      421 gagcaggcgg ctttgatcat caagatgtcc ggggcctcag ccgtgattgc cagatcgttc
      481 gcgcggattt tcttccgcaa ctcgatcaat aacggcctgc ccttgattga aaccgacacc
      541 acaggcattg acgacggcga cattctcgaa gtggaactga acgcggggaa actctcaaac
      601 gtgaccaggg gctgggagcg gcagttcaac cccctgccga agattatgac cgatatctta
      661 gccgacgggg gcctggtggc gcacatcaaa aaacacgggg attttaattt gtaaactaaa
      721 cacaattgct ttcggtcaga agccaatgga aaagacggat gtgctctcgt acataaaggg
      781 gtgtagtcag gaaattgcta cccgctttga tgaactgttc tctggagaaa tggaatcttt
      841 tgctactgaa atgacttcta ctttttccat cttacaaaag gctcgtaatt acactaaaca
      901 acccggggca tctgaaatag atcataattc cgttatgatt ctctggagca aggcaaattc
      961 tctgattagt gcatttgaat tagtacgccg aggatatatt gtcgagcctc taatattggc
     1021 acgacacgcg ctggaaggag ctgccgtagt tgtggacatt ctaaaatccc cgcagaaact
     1081 ttcaaagttc ttttcccaaa aatacaaatc aactggtgca atcagcgtcg caaaagaagt
     1141 tattccgatt tttgggaaaa tttatggtgg cctgtcagat aatcttgtcc atattggact
     1201 tctttcttgt atgcctcagt gggggatggg gccaaacttt gggattcagt cacttttttt
     1261 gggaggtggg attgatgaga ataggataga ggattatgta atcgttttgt gttcgttgga
     1321 tctttgtaca gatgttttta acacgatagt tgaatattcg ctatttaagt ttgttgattc
     1381 acccagattc tggagaaaaa agagtgagac cactctttct ttcgagcctg acgatgtaat
     1441 aaaagatcgc catgaagaaa gacaaagaaa gttggtagaa gcatttcaaa aattaatggg
     1501 tcgagtcgaa aagcaacagg agatattgtc aagaatagat ggtggaaacg attctaacac
     1561 cctcttgccg ggttaaaagt ccagcccttt tctgaaaagt atcgaaggac tacttaatat
     1621 gactttggaa attggggaga ggtaaccaag tgaaactaag acaacgcctt atccttattt
     1681 ttggcttcat tctgataatg ttacgattcc tatttcctcc tgtagaaatc tccctagcag
     1741 atcgagtaga agaagaaaaa cgaaccaaag aactgatggc gaactttgac gatctaatta
     1801 aaggtatgac ggaacttagc ggaggtgaaa taacccctcc catgcaagac cttattaatg
     1861 ctcatcaaga gttagttaat aaacgtgggc gttttatggc tacctattcg agccgaccgt
     1921 ttcacttttt gttcaacacg ccacctcgtc cagcgaagtt tgtgtggaat gtagtaatta
     1981 tccacgtttt aattattact attcttgcgt ggtttggata ttggattttg aagcgatctc
     2041 ctcggagtaa tccgaggtaa ggggcctcgg ggatccaggt tatggaccta aaaagaattt
     2101 ctctcttttc actccgtgcc tccgtcgtgc atttttgtat ttgaatcctc ccgcaattct
     2161 ctctatcttc gctctgcatg cacaagatcg ccgttgtctc cggggatggc agcgggccgg
     2221 aagcgggtca gaacagtagc gaagcctcgg ccatagagga ggttcgtcgc aatgaaaatc
     2281 aaaactaaat tttggtcagc agccgtggga tcactcccat tgatgttagg cgtttacctt
     2341 cccgacagca ccgtccattc cctcaaagtt gccgggggca ccggacagta cgccacgatc
     2401 tttcgcgact gtgcgggaaa cccgacgagt agtcacggga tcccgtacaa agacgtcggc
     2461 ggcgagtact cgttttctcc acccggcgca gccagcttgg ggctccgggc aagccgcgtt
     2521 tgggatcgca cggtgacagg gagttttccc gatacttttc tcttgcgccg tgagagaaca
     2581 attatcaacc cattcttcca gctggattgg cggaaattcg ggatcggcgg cggtgtcgcc
     2641 ttcaacgggc gccagaataa agtgaccggt gaaaagagcg ccatgccttc gtggtatcta
     2701 cggatagcca gagggccgat ctatttttct tcgaccttct tctgcggtgt tccactttac
     2761 tcgggcgggg gcggtctcaa t
//
LOCUS       DBNGZD010000099         1355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1055507, whole genome shotgun sequence.
ACCESSION   DBNGZD010000099 DBNGZD010000000
VERSION     DBNGZD010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1055507"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16GM_02675"
     CDS             complement(<1..240)
                     /locus_tag="AC16GM_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922611.1"
                     /translation="MGARVISQPSNAAGAPLAASFGWPPWVKFHEQSGAPWLEFEPLK
                     RVPDLLHGFTLRSSNPASSENRREQLKEHLQRIGLP"
     gene            complement(225..>1355)
                     /locus_tag="AC16GM_02680"
     CDS             complement(225..>1355)
                     /locus_tag="AC16GM_02680"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2922612.1"
                     /translation="TQRHPEVEIHLCDRNTYHLLETRLHEAAMRKAEVAIPIARIIRR
                     WNVHFHLGEVSQIDLSVRQVVLGEKTLAYDYLVIALGSKTNFYAIPGLAELALQLKTL
                     EDSLKIRQRVDVMFARARSEKDPEKRRRLLTFVIGGGGLTGAELAGEMTERVEKHCHD
                     YEIPRGEVQIILLEAGPRIVPSLSEKQSALSVERLQSRGVRILTNTKVVGMEPGKVLI
                     EPGEPIPAETLVWTGGIRASRLLAGSGLPVGVQGRILVDEYLEVKGFPGVFAVGDNAL
                     AINPKTGKTVVAAAQFALQQGRLVAWNIFAEMFGKPKKVYVPKVFGEVISLGRHLAVG
                     WIALPGIKRIRFFGFLASLLKRAITEKHLLLLWRETRNWARA"
BASE COUNT          281 a          398 c          378 g          298 t
ORIGIN      
        1 gggcaggccg attcgttgga ggtgctcttt tagttgttcc cggcggtttt ctgacgaggc
       61 aggattactt gagcggagag tgaatccgtg cagcaaatcg ggaactcgtt tgaggggctc
      121 aaactcaagc caaggcgctc cgctttgctc gtggaatttc acccagggtg gccagccaaa
      181 acttgcggca agtggtgctc cagccgcgtt tgacggctgg gagattacgc gcgcgcccaa
      241 ttcctggtct ccctccagag caacaggaga tgcttttcgg tgatggcccg cttgagcaaa
      301 ctggccaaaa aaccgaaaaa tcggatgcgt ttgatacccg gcagggctat ccacccgacc
      361 gccaagtggc gccccaaact gatgacctcc ccgaagactt tgggaacgta cactttcttc
      421 ggttttccaa acatctcggc aaaaatgttc caggccacca gccgtccttg ctggagggcg
      481 aactgggccg cagccactac ggtttttccg gtcttcggat taatcgccag ggcgttatcg
      541 ccgacagcga agacgcccgg aaatccttta acttcgaggt actcatccac cagaatgcgt
      601 ccctgaacac caaccggcag tccgctgccg gccagaagtc ggctggcccg tatgcccccc
      661 gtccagacca aagtctcggc tgggattggc tcgcctggtt caatgaggac tttgcccggc
      721 tccataccca ctactttcgt attcgtcaga atacggacgc ctctggactg gagccgttcg
      781 acgctcaaag cgctctgttt ttccgaaagc gaaggcacga tgcgcggccc ggcttccagg
      841 agaattattt gaacttcacc ccgcggaatt tcgtagtcgt ggcagtgctt ttccacccgc
      901 tcggtcatct ccccggctaa ttcggcgccg gtcaatccgc cgccgccaat gacaaaggtc
      961 aaaagccggc ggcgtttctc tgggtctttc tctgaccggg cccgggcgaa catgacgtcc
     1021 acccgctggc ggattttaag ggaatcctcc aaggtcttca gttgcagcgc aagttcggcc
     1081 agaccgggaa tggcgtagaa attcgtcttg ctccccaaag caatcaccag gtaatcgtag
     1141 gccaaagttt tttcacccag tacgacctgt cggacagaga gatcaatctg actcacttcg
     1201 cctaaatgga agtggacgtt ccagcgccgg atgatacggg cgatcgggat cgccacttcg
     1261 gctttgcgca tcgcggcctc atgcaggcgg gtttctaaaa gatggtaggt attccggtcg
     1321 caaaggtgga tttccacctc ggggtgacgt tgggt
//
LOCUS       DBNGZD010000100         2814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1128008, whole genome shotgun sequence.
ACCESSION   DBNGZD010000100 DBNGZD010000000
VERSION     DBNGZD010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1128008"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16GM_02685"
     CDS             complement(<1..307)
                     /locus_tag="AC16GM_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR2922613.1"
                     /translation="MAKVKLPVAVGFFNDPQDLLEAARQAKERGFANLDAYTPFNVHG
                     LDRALGLKPSWVPFATLVAGLSGAALGLYFMYWTSTVNWPLIVGGKPFFSWPAFVPIT
                     "
     gene            complement(300..1721)
                     /gene="nrfD"
                     /locus_tag="AC16GM_02690"
     CDS             complement(300..1721)
                     /gene="nrfD"
                     /locus_tag="AC16GM_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR2922614.1"
                     /translation="MTPVVGQLPLSPAPLAPQREPLVRADIALSDVTRDVLAPLDRKP
                     GPLWLIGFLLAITGVAIGTYVVYNELKIGLGIFGWTHPVMWGVFITNFVFWIGIGHAG
                     TLISAILYLLRQRWRTSINRSAEAMTIFAVICAALFPVLHIGRSWFAYWVIPYPNTRH
                     LWVNFRSPLVWDVFAISTYFTVSLMFWFQGLVPDFATIRDRTTSALKKKIYGLLSLGW
                     AGSVRDWGHYEKAYLLFAGLATPLVLSVHSIVSFDFAVSLMPGWHTTIFPPYFVAGAI
                     FSGFAMVMVLTVVMRKAYHLEEYITLNHLELMNKIIVVTSLMVGYAYLTEAFMAWYSE
                     NPFERHMLANRATGHYAWCYWTMVLCNVLIPQALWFKKVRRNIPLMFAISFLINIPGM
                     WLERFTIIVTSLQHDFLPTTWGNYTPTAFDWGITLGSFGLFFTLFLLFCRFFPTISLA
                     EVKSVLLRPAGRKSRPPAEAAHG"
     gene            complement(1718..>2814)
                     /locus_tag="AC16GM_02695"
     CDS             complement(1718..>2814)
                     /locus_tag="AC16GM_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2922615.1"
                     /translation="ESEVVTLTANGSSLDLPVHLQPGVHPQVLAVAVGWGREKVGRYG
                     NKVGVNAFPLAALKENALHFSAIPVAVKKTGKKQSLACVQGHHYMESRPIIYAAALEE
                     YRKNPTAGREAEEKLPSIWKAHEYTGYRWGMAIDLNSCIGCNACMVACQAENNVAVVG
                     KAEVLKGREMHWIRIDRYFSGHPDHPEVVHQPMLCQHCEQAPCETVCPTLATVHNEEG
                     LNLQIYNRCVGTRYCSNNCPYKVRRFNWYENFWNIPTPLELVLNPDVTVREKGVMEKC
                     TFCIQRIREGKEKAKEKGIAVADGDIQTACQQTCPTRAIVFGDINDPDSAVSKMSRDP
                     RGYHVLEILNTKPSIAYLTKIRNRRGNGAA"
BASE COUNT          627 a          781 c          865 g          541 t
ORIGIN      
        1 aggtaatcgg cacgaaggcc ggccaggaaa aaaacggttt gccgccgacg atcaagggcc
       61 agttcaccgt cgaagtccag tacataaagt agagtcccag cgcggcaccg gagagccccg
      121 ccacgagcgt ggcaaacggc acccaggaag gtttcagacc caacgcccga tccagaccat
      181 gtacgttgaa gggggtgtag gcatcgaggt tagcaaagcc gcgctctttc gcctggcggg
      241 cggcttccag caggtcctgc gggtcgttaa agaaaccgac cgccaccggc aattttacct
      301 tagccatgcg ctgcctccgc aggcggacgc gactttcttc cggcgggccg caggagcacg
      361 cttttcactt cggccaggga aatggtgggg aagaagcggc agaaaagcag gaaaagagtg
      421 aagaagagac cgaaagaacc gagcgtgata ccccaatcga aagccgtcgg cgtatagttc
      481 ccccaggtag tcggcaaaaa gtcgtgctga agagaggtga cgatgatggt gaaacgctcc
      541 agccacattc ccgggatatt gatcagaaaa gaaatggcga acatgagcgg gatgttgcgc
      601 ctgactttct tgaaccagag cgcctgaggg atgaggacgt tacagaggac catcgtccag
      661 taacaccagg cgtaatggcc ggtggcccgg ttggccagca tgtgccgctc gaatggattt
      721 tcactgtacc aggccatgaa ggcttcggtg aggtacgcgt accccaccat gagggaggtc
      781 accacgatga ttttattcat caactccaga tggttcaagg ttatgtactc ttccaagtgg
      841 taggctttgc gcatgactac cgtcaagacc atcaccatgg caaagccgga gaaaatcgcg
      901 ccggccacaa agtacggggg gaagatggtg gtgtgccagc cgggcatgag cgagacggcg
      961 aaatcaaacg acacaatgct gtgcaccgag agcaccagag gcgtggccag gccggcgaag
     1021 agcaagtagg ccttctcgta gtggccccag tcgcgcaccg aaccggccca gcccaaagag
     1081 aggaggccgt aaattttttt cttcagggcg cttgtggtcc ggtcccggat ggtggcgaag
     1141 tcggggacca gtccctggaa ccagaacatg agggagacgg tgaagtaggt ggaaatggca
     1201 aaaacgtccc acactaaggg ggagcggaag ttgacccaga gatggcgggt gttgggatac
     1261 gggatcaccc aataggcgaa ccagctgcgg ccgatgtgca agaccggaaa gagcgccgca
     1321 caaatcaccg caaaaatcgt catcgcctcc gccgagcggt tgatcgaggt gcgccagcgt
     1381 tgccgcaaga ggtacagaat ggcggaaatc aaggttccgg cgtggccgat cccgatccag
     1441 aagacgaaat tggtgatgaa cacgccccac atcaccgggt gggtccagcc gaaaattccc
     1501 agcccgattt tgagctcgtt gtatacaacg taggtgccaa tggcgactcc ggtgatggcc
     1561 aggagaaaac caatgagcca gagcggaccg ggtttacggt ctaaaggcgc cagcacgtca
     1621 cgggtcacgt cgcttaaggc gatgtcggcg cggactaagg gctctcgctg aggtgccagc
     1681 ggagcaggag agagcggcag ttgtccgact accggcgtca tgcggctccg ttcccccggc
     1741 ggttgcggat tttcgtcagg taggcgatgg aaggtttggt gttcaagatc tccagcacgt
     1801 ggtacccgcg cggatcgcgg gacattttcg acacggcact gtcgggatca ttgatgtcgc
     1861 cgaaaacgat ggcccgggtc gggcaggttt gctggcaggc ggtctggatg tcaccgtcgg
     1921 ccaccgcaat acctttttct tttgcctttt ccttcccttc ccgaatcctt tgtatgcaaa
     1981 aagtgcactt ttccatgacc cctttttcgc gcaccgtcac gtccgggttc aagaccaact
     2041 ccagcggggt ggggatgttc cagaaattct cataccagtt gaagcggcgc actttgtagg
     2101 ggcagttgtt ggagcagtag cgggtgccga cgcagcggtt gtaaatctgg aggttcaaac
     2161 cttcttcgtt atgcacggtc gccagcgtag ggcaaacggt ctcgcagggc gcctgttcac
     2221 agtgctggca gagcatgggc tggtgcacca cttccgggtg gtccggatgg ccggagaagt
     2281 aacggtcgat ccggatccag tgcatctcgc gccccttgag cacctctgct ttgcccacca
     2341 ccgccacgtt attttccgcc tgacaggcca ccatgcaggc gttgcagccg atgcaggagt
     2401 tcaggtcaat cgccattccc cagcggtagc cggtatactc gtgcgccttc cagatcgaag
     2461 gcagtttttc ctcggcctcg cggccggcgg tcggattttt ccggtactcc tccagcgccg
     2521 ctgcgtaaat aatgggccgg ctttccatgt agtggtgccc ctgcacgcag gccagagact
     2581 gttttttccc ggttttcttt accgccaccg gaattgcgga aaagtgcagc gcattttctt
     2641 ttaatgccgc caggggaaag gcgttgaccc ccactttgtt tccatagcgt ccaacttttt
     2701 cgcggcccca gcccacggcc accgccaaaa cctgaggatg gacgccgggc tgaaggtgca
     2761 ccggcagatc gagacttgag ccattggcgg tcagcgttac aacctccgat tcgg
//
LOCUS       DBNGZD010000101         8307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1055596, whole genome shotgun sequence.
ACCESSION   DBNGZD010000101 DBNGZD010000000
VERSION     DBNGZD010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1055596"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC16GM_02700"
     CDS             <1..210
                     /locus_tag="AC16GM_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2922616.1"
                     /translation="EADQPLPARLKTCRKGLGLSQKRLAKILRVDPTTVASWEAATNR
                     PTAKSIKLIDSFLTSQTLAALVSSE"
     gene            complement(614..1513)
                     /locus_tag="AC16GM_02705"
     CDS             complement(614..1513)
                     /locus_tag="AC16GM_02705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR2922617.1"
                     /translation="MWVYIIGYFSLLALLYYREHFSPTEPLLVLLVVGGLFSLLAVWL
                     TRGRKAHSFPVNHPRAESRLLVGYLFVIVAFLTWGIPFLRTLGSTQFSSSLALLTGKL
                     LVFVVVPLILWKFLWGYTPACLLGLPIRLKGHWRPVLGMAGVLIVFQLVFGRALKALA
                     ETAHSPVVLGIASFLAFGWLLVEVGLVEEFFFRGLLQTRLAVWTGSECTAIVLVAVLF
                     GLAHAPGLYLRPETTGEALGTAPSLIKAVGYAITVTSVTGFFLGVLWARLRNLAALII
                     IHTAGDFFPNLVDFVRMVSQHSR"
     gene            2349..3536
                     /locus_tag="AC16GM_02710"
     CDS             2349..3536
                     /locus_tag="AC16GM_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2922618.1"
                     /translation="MSSALRFDIRRGAGLAALLILSFSFSSASGQFTLLYQKNGAAAS
                     DFFGRSVSSAGDVNGDGKADFIIGAYNTAPRDINGIGAGSAYVYSGVDGALLFQRNGA
                     AAGDLLGYSVASAGDVDGDGKADFIIGAPQAHVGSFINNIGSAYVYSGATGALLYQKD
                     GATTGDLFGTSVASSGDLNRDGKSDFIVGANNAQPGGLTSAGSAFVYSGATGALLYQK
                     NGAAAFDNFGYSVASAGDVNGDGKPDFIIGANVADPGGLTSAGSAYLYSGTTGALLLQ
                     KDGATTGDQLGIYVASAGDVNGDGRADFIIGAHAASPGGLFNAGSAYVYSLPCVVRGD
                     VTNDAATDIVDIVALVQNVVFGTPLPNPLAGDVNCDTVRDVTDIVSLIQYVVFGTPTP
                     CCL"
     gene            3551..>8307
                     /locus_tag="AC16GM_02715"
     CDS             3551..>8307
                     /locus_tag="AC16GM_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HIR2922619.1"
                     /translation="MTKRVACYAGFACFLLTSYFVLHGRTAAEQKKSGRSVLEQLLAR
                     GVDPRVSKIIPETLVVAPQPALIRREIPVPTEPRTVRLRGQTATVYPNIQMHPSTTTT
                     QSEMSIATDPVNPNVVLAGSNAVSVFVDFGSQGWYHTTDGGVNWKGGDTLPTHTNPST
                     FMSDPAVGIDLGANLFFSALLLGAQSSDVVIAKSTNGGAVWTQTFVPDPSNDQDKDHF
                     TVDVNPSSPHAGNLYAAYTDFEEVVPPIRFSRSIDGGATFSSPVSISNGVGKLFNQGV
                     NMAVGPDGELYAVWTGYDFFPLQPGDSTRLGFNKSTDGGQTWQAAKTIRNVADIRGYL
                     NKGGNIIRVASFPSMAVDRSAGPRRGWIYVVYAQIATTTLTADVFLIRSTDGGTTWNS
                     PKKVSQENTATDQWFPATAVDPATGVLYVDYYDSRNFADDSAQVYVSISSDGGDTFQD
                     VLVSDAPFLPASIPGLAGGYMGDYIGIAAVNGTVWPSWNDNRTGIHQAYTARMVTSTV
                     SFPAKIRVSPDTLDFGARFIGFPETLAVLVRNLGTPDTLVVSGIGSDNPLFVPDADSF
                     SLTGGAFRLVNVVFSPVAPGSFTGHLSISSSDSTQPLVTVALRGSAALPPQLAVLPDS
                     LRVDLTTGESKAETLLISNPGGSDLSFQILKGAAGPALQANFNAAPSLPTASPLATGR
                     PVTLSELKEMQAGIPETLQNFPDKLPDAIPGYNPTVPLEFSGAAAADSVEIFGGLQFP
                     FTGGFRLRGNMFFCTDSANLTEHRFWLKTFGPTDLWFLVYEGTRPPLPQFFSAGRLIS
                     ASYVKTSGAAEGWYSSGNVDVPLTPGHHYLIVTQFRQACTYYYQLCTSPCVSYPISTS
                     FGRLEGPILLGNWNGGPIYAVPPETLQFVPWYALSLQGAYSAHQTLVTGPGTSWLAAS
                     ADTGTVPPGGSRALPVVFDSKAFGSPSVGAGDYFANLIVSANFPATPAPAETVKVHLH
                     LTAAPNLVFFGDSLDFGEAFVGFPDTLPFIVSNTGAETLRVSGITTSNPRFTLLNSPV
                     FNLLPGQSRTLQVQFLPSGLGVETALLSISSNDPNEPVKNGFLKGAAVHPPVLSPAPD
                     SFAVTLNQGKSLATTLSLHNTGLGTLSFSLHISPRPKVLLLSTDIDPFQIGDVQAKLL
                     ATGRFSVVDTIQLRFVDLPTMDQLRQYDAVLIWSGNLFMQPSAAAVGDLLADYVDMGG
                     GVVTAFFAMDNDPRFPPGNMIEGRFNAENYWALQPGPPKRGRATLGTIHLPGHPTIQG
                     VKLFDGGDFSDRLQGGTVALGGTLVASWSDGEPLVAFKGKKRVDLDFYPVSDSANCYQ
                     CNIGRWVSTTDGAILMADALEYVAGIAALAPNAGTAPPGGTANLSLSLAADVPRPGAY
                     CADLIISSNDPTRRVDTIPVSLTVPAGPNLLPAKSSLDFGDAYVGFPDTLALRVYNNG
                     SAPLSVSGINATNPRFSVLGSTAFSVLPGQFQDLKARFLPTSVGTDTGTLAIASNDAA
                     DPTVAVPLQGKGLLAPNISFSADSIFSTLLPGGKSALTLIIHNPGATDLKFSASISRT
                     PPVEALLLRNGVASLVPTTEKVAAPGQVIRTILP"
BASE COUNT         1755 a         2493 c         2285 g         1774 t
ORIGIN      
        1 gaggccgacc agcccctacc tgcccggctc aagacctgcc ggaaaggact tggactgtcc
       61 caaaaacgct tggccaaaat cctccgggtg gacccaacca ccgttgccag ctgggaggcc
      121 gcaacgaacc ggccaaccgc caagtcgata aaactcattg atagctttct cacttctcaa
      181 actctggccg ccttagtctc ttccgaatga cctaagttct aaagttacgg ccatcgtttt
      241 aaaccgtacc gaggcgttgg acatcagcga aaagccttcg ggaggttggc gaaaacccct
      301 tgtctgcccg ctctcattgg actgagcaac gctaaggctg agcaagtgag gccataggcg
      361 tcggtcaaca aacgtgtctt tgctagtaag aagcttggtg atcaggacgt ctatatgatc
      421 ggtgagctcg caaagaacac tgaagatccg aagaactcgt ggatgcgacc atcactagtt
      481 tcggagacac tcccctgtta gtagtgaagc tacgttcacg agcgtagggt cttgtttgag
      541 cagtatcccg tgggcctcga gcactcggag aatgtagcgg ttctctcgtg caaggcggtc
      601 taaagcggca ggttcatctc gagtgttggc tcaccattcg aacaaagtcg accaagtttg
      661 ggaaaaagtc gccagccgtg tgaatgataa tcagtgccgc gaggttacgc aaccgtgccc
      721 acagtacacc aagaaagaag ccggtgaccg acgtaacggt aatggcatat ccgacagcct
      781 tgataaggga gggtgcggta cctagcgctt caccggtcgt ctcagggcgc aagtagagac
      841 cgggcgcatg ggctaggcca aagaggactg cgacaagaac gatagcggtg cactccgatc
      901 cggtccagac ggcaaggcgg gtctggagaa ggccccggaa gaaaaactcc tctaccagac
      961 caacttcgac caaaagccag ccaaacgcga ggaaggaagc tattccaagg acaacagggg
     1021 aatgggcggt ttctgctaaa gctttgaggg cacgaccgaa gacgagttga aagactatca
     1081 gcacgccagc catcccaagc accgggcgcc agtgaccttt gaggcgaatc ggcaaaccga
     1141 ggaggcaagc cggtgtgtac ccccagagaa acttccagag gatcaatgga accaccacaa
     1201 agacaagcag tttgccagtg agaagggcaa ggctggagga aaattgtgtg cttcccaggg
     1261 tacgtaggaa gggaatgccc caggtgagaa aagccacgat aacaaagaga tagccgacca
     1321 gtagccttga ctccgccctt ggatggttca ctgggaaaga gtgggctttt cgaccgcgag
     1381 tcagccacac tgccaaaagg gagaaaagtc cgcccacgac gagaaggaca agaagcggtt
     1441 ccgtagggga gaagtgttcg cggtaataaa ggagagcgag gaggctgaag tagccgatga
     1501 tgtagaccca cacggcaacg tgacgaagga aatgtgaggg cggtagcatt ttcgatgtcc
     1561 tttctccgtc agggtttacg cacgtccagc actaatagcc ggccggtcgc gtagtcaacg
     1621 gtcaggataa tctatgttag aatgtcagag tcgatcgaga gctgtacccg cggtgataga
     1681 gtcaagtcga tgcccgaccg gacatgctct atgacgacgg caccacattg atgagcggac
     1741 caatagcaag tgagtcaatg cgtaccccat tgactgctcc tgcaatcgtt tgatgcacct
     1801 tcgtcttatc taaaaccggc aaaaggacgc acaggtttca cgtctggagc ggaatataat
     1861 caatgcccgg ggatgcggca gagaaatttg cgtccaggga gcataaacgg gaaaacctgt
     1921 ccccaaattt gaagcgatcg gttcggcccc cccccgacat cgctggttcg attccgcctt
     1981 aggcggattc acctcaactc gggacaagtt cgctaaccgc aagtacagta ccgctatggg
     2041 aataaagtga atacattgcc tcccaggaac aatctgacat aaggtatggt ttaaggtagt
     2101 gatacgcgca ttctgtttag gatgggcagt gccgaatcgg gtgtccccgc cgaagggagg
     2161 ctgtgagcta cgaggccaag agcccggaaa attatgatta aaataatata aaaaggtaat
     2221 atagtccttg acaagactaa actggtagag tattttctcg taagaacact tgctgcaact
     2281 cggtctgaga aaagagacgc gtttgggaag ttaacggcag cattcaccca actttatctg
     2341 gaggtcgtat gtccagcgct ttacgcttcg atatccggcg cggtgcagga ttggcggctt
     2401 tgctcatcct gtcattttct ttctcatccg cttccggcca gttcaccttg ctgtaccaga
     2461 aaaacggcgc cgcggccagc gacttcttcg gccgctcggt gtcttcggcc ggcgacgtga
     2521 acggagatgg caaagccgat tttatcatcg gcgcttataa caccgctccc cgcgacataa
     2581 atggtattgg agctggttcg gcttatgtct attccggagt tgacggcgca ctccttttcc
     2641 agagaaacgg cgccgcggcc ggggacttgc tgggctactc cgttgcctcg gccggcgatg
     2701 tggatgggga cgggaaagcc gattttatca tcggggctcc tcaagcccac gtgggcagct
     2761 ttataaataa catcggctcg gcctacgtct attccggcgc caccggcgcc ctgctctacc
     2821 agaaagacgg cgccacgacc ggtgaccttt ttggaacctc cgtggcctcg tcgggagatc
     2881 tgaacagaga cgggaagtct gattttattg ttggggctaa taatgcccag ccgggcgggc
     2941 ttacctcggc cggctcggcg tttgtctatt ccggcgctac cggcgccctt ttgtaccaga
     3001 aaaacggcgc cgcggcattt gacaactttg gctattcggt cgcctcggcc ggagacgtta
     3061 atggggacgg gaaaccggat ttcattatcg gggctaatgt tgccgacccg ggcgggctta
     3121 caagtgccgg ctctgcctat ctttattcag gcaccaccgg cgctttgctt ctccagaaag
     3181 acggcgccac gaccggtgac caactgggca tttacgtggc ctcggccggg gatgtgaacg
     3241 gagacgggag ggctgatttt atcatcgggg ctcatgcggc cagtccaggc gggcttttta
     3301 atgccggctc ggcgtatgtt tacagtctgc cgtgcgtggt gcgcggcgat gtgacgaacg
     3361 acgcggcgac cgacatcgtg gatatcgtgg ccctggttca gaacgtggtg tttggcacgc
     3421 ccctgcccaa tccgctcgcc ggtgacgtca actgcgacac ggtgcgggac gtcaccgaca
     3481 ttgtatcgct catccagtac gtcgtttttg ggacacccac gccctgctgt ttgtgaaaaa
     3541 aggagtagtt atgacaaaaa gagttgcctg ctacgccggt ttcgcctgtt tcctcctcac
     3601 ctcgtacttc gttcttcacg gccggacggc agccgagcaa aaaaaatcag gccgttcggt
     3661 tttggaacag cttttagccc gcggggtcga ccctcgagtc tcaaaaataa ttcccgaaac
     3721 tcttgttgtc gcgccacaac ctgctctcat tcgacgtgaa atcccagtcc ccaccgaacc
     3781 gcggacggtc cgtttgcggg ggcaaaccgc caccgtttac cccaacatcc aaatgcaccc
     3841 cagcacaacc accacccaga gcgagatgtc gattgcgacc gacccggtca atccgaacgt
     3901 cgttctggct ggctctaacg ccgtcagcgt cttcgtcgat tttggcagtc agggatggta
     3961 ccacaccacc gatggcggcg tcaactggaa gggcggcgat acgcttccga cccacaccaa
     4021 cccctcgacg tttatgtccg acccggcggt cggcatcgac ctcggcgcga accttttttt
     4081 cagcgccctt cttttgggcg cccaaagttc ggacgtggtc attgccaaat cgaccaacgg
     4141 cggagcggtc tggacccaga ccttcgtccc ggatccaagc aacgatcagg acaaagacca
     4201 tttcaccgtg gacgttaacc cttccagccc gcatgccggc aacctttacg ccgcttacac
     4261 cgattttgaa gaggttgtgc cgcccatccg tttctcccgt tccatagatg gcggagccac
     4321 cttttcaagc cccgtttcca taagcaacgg agtggggaaa ctcttcaacc agggagtcaa
     4381 tatggcggta gggccggacg gagaactgta tgccgtttgg accgggtatg acttttttcc
     4441 gctccaaccc ggcgattcca cccggctggg attcaacaaa tcgaccgatg gcgggcagac
     4501 ctggcaggcg gccaaaacca tccgcaatgt ggccgacatc cgcggttatt tgaacaaggg
     4561 gggaaatatc atccgggtgg cgagcttccc gtccatggcg gtggaccgct cggccggccc
     4621 cagaagggga tggatttatg tggtctatgc gcagatagcg acaactacgc tcaccgccga
     4681 cgtttttctg attcgttcca ccgatggcgg tactacttgg aattccccca aaaaggtgag
     4741 ccaggaaaac accgctactg accaatggtt cccggcgacc gccgttgacc ccgccacggg
     4801 ggtcctttat gtggattact acgacagccg caattttgcc gacgactccg cccaagttta
     4861 tgtcagcatc tccagcgacg gcggcgacac cttccaagat gttttggtca gcgacgcgcc
     4921 ttttcttccc gcttccatac cgggcctggc gggaggttat atgggggact acattggcat
     4981 tgccgccgta aacgggacgg tttggccctc ctggaacgac aaccgcaccg gaatccatca
     5041 ggcctacact gcccggatgg tcacttcgac cgtcagtttc ccggccaaaa tcagggtctc
     5101 gccagacacg ctcgattttg gcgcccgttt catcggcttt cccgaaaccc tggcggtatt
     5161 ggtgcgcaat ctgggaacac cggatacgct ggtggtatcg ggcattggtt cggacaaccc
     5221 cctgttcgtt ccggatgcgg acagcttttc ccttacggga ggcgcgttcc gtttggtcaa
     5281 cgtggtgttc agcccggtgg cgcccggttc tttcaccggc catttgagca tctccagcag
     5341 cgactccacc cagccgctgg taacggtcgc cctgcggggc agcgcggcgc tccctccgca
     5401 actcgccgta cttcccgatt ccttgcgggt cgatttgaca accggcgaaa gcaaggccga
     5461 aacgcttttg atttcaaatc cgggaggcag cgacctctcc tttcagattt taaagggcgc
     5521 cgccgggccg gccctccagg caaacttcaa tgcggctcca tccctcccga ccgcctctcc
     5581 cctcgccacc ggccgcccgg tcaccctgag cgagttaaag gagatgcagg ccggcatacc
     5641 ggaaacgctc cagaattttc cggataaact tccggacgcc attccgggtt ataacccaac
     5701 cgttccgctg gaattctccg gagctgcggc ggccgattcg gtggaaattt ttggagggct
     5761 gcaatttccg ttcactggcg gattccggtt gcggggcaac atgtttttct gcaccgactc
     5821 cgccaatctg acggaacacc ggttttggct gaagaccttc ggcccgaccg acctgtggtt
     5881 tttggtgtat gaagggaccc gcccccccct gccgcagttt ttcagcgcgg gacgcctgat
     5941 cagcgcatcc tatgtcaaaa cctccggcgc ggcggaggga tggtacagtt ccggcaacgt
     6001 cgacgttccg cttacccccg gacatcatta tctgattgtt acccaatttc ggcaggcctg
     6061 cacttactac tatcagttgt gcacctcccc ctgcgtttcg taccccatct ccacctcatt
     6121 cggccggctg gaaggcccga ttcttctggg caactggaat ggcgggccga tttatgccgt
     6181 cccgcccgag accctacagt tcgtcccctg gtacgcgctc tccctgcaag gcgcctattc
     6241 tgcccatcag acactggtga ccgggccggg cactagctgg ctggcggctt cggccgatac
     6301 cggcacggtt ccgcccggcg gcagccgggc cttgccggtc gtttttgatt ccaaggcctt
     6361 tggctccccc agcgtgggag cgggggacta ctttgccaat ctaatcgtct ccgccaactt
     6421 tccggccact ccagccccgg ccgaaacggt aaaggtacat ctacatttga ccgccgcccc
     6481 taacctcgtt ttctttggcg actctctcga ttttggagag gcgttcgtgg gttttccgga
     6541 cacgcttccg tttatcgtct ccaacaccgg cgccgaaacg ctgcgggtga gcggaataac
     6601 cacctccaac ccccgcttca ctctcttgaa ctctccggtt ttcaacctgc ttccgggcca
     6661 gtcgcgcacc ttgcaggtac aatttctccc aagcggcttg ggagtcgaaa ccgctcttct
     6721 ctccatctct tccaacgacc caaatgaacc ggtgaaaaac ggatttctca aaggggcggc
     6781 cgttcatccc ccggtccttt ctcccgcgcc ggactccttt gcggtgacct tgaatcaggg
     6841 aaagagcctg gccacgacct tatctcttca caataccgga ttgggaacgc tttccttttc
     6901 tctccacatt tccccccgtc caaaagtcct gcttctttcc accgacattg acccattcca
     6961 gattggggat gtccaagcca agctccttgc caccggacgg ttctccgttg tggatacgat
     7021 ccaacttcgt ttcgtcgact tgccgactat ggaccaactc cggcagtatg acgccgtctt
     7081 aatctggagc ggcaatctat ttatgcagcc cagcgcggcg gctgtcgggg acttgctggc
     7141 ggattacgtc gatatgggcg ggggcgtggt aaccgccttc tttgcgatgg acaacgaccc
     7201 cagatttccc cccggcaata tgattgaagg ccgctttaac gccgaaaatt actgggcgct
     7261 ccagccgggg ccgcccaagc ggggcagggc caccttgggg accatacatc ttcccggcca
     7321 cccaaccatc cagggtgtaa aactttttga cggcggggac tttagcgacc ggctgcaggg
     7381 gggcacggtc gctttgggcg gcacgctggt cgcttcctgg agcgacggcg aaccgctggt
     7441 ggctttcaag gggaaaaaac gggtggactt ggatttttat ccggtctcgg actccgcaaa
     7501 ctgttaccaa tgcaacatcg ggcggtgggt ctccaccacc gacggggcca ttttaatggc
     7561 cgatgccctg gaatacgtgg ccggcatcgc cgctctggca cccaacgccg gaacggcgcc
     7621 tcccggagga acggcaaacc tttccctatc gcttgccgcc gacgtgccaa gacccggtgc
     7681 gtattgcgcc gacctgatta tttcgagcaa cgacccgacc cgccgggtgg acaccatccc
     7741 ggtttccctg acggtacccg caggtcccaa cctgcttcct gccaaatctt cgctggactt
     7801 tggcgacgcc tatgtcggat ttccggacac gcttgcgctc cgcgtctata acaacggttc
     7861 ggcgccgctt tcagtcagtg gtataaacgc aaccaacccc cgcttttcag tcctcggttc
     7921 gacggccttc agcgttttac ctggtcagtt ccaggatttg aaagcgcgct tcctcccaac
     7981 atcagtcggg acagacaccg gaacgctggc gattgcgtcc aacgacgcgg ccgacccaac
     8041 cgtggccgtg cctctgcagg gcaaagggct cctggcgccg aacatttctt tttcagccga
     8101 ttctatattc agcactcttc tccccggtgg aaaaagcgcc ttgaccttga tcatacataa
     8161 ccccggcgcc accgacctga aattctccgc ttcgatcagc cgaacccctc cggtggaggc
     8221 gctactcttg cgcaacgggg ttgcatcgtt agtgcctacc actgaaaaag ttgccgcccc
     8281 cggtcaggta attcgtacca ttctgcc
//
LOCUS       DBNGZD010000102         4618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1128137, whole genome shotgun sequence.
ACCESSION   DBNGZD010000102 DBNGZD010000000
VERSION     DBNGZD010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1128137"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="AC16GM_02720"
     CDS             complement(<1..367)
                     /locus_tag="AC16GM_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR2922620.1"
                     /translation="MPLIGKAWKFGDNISTDLIAPGRYFHLRSNLEEFAKHVMEDADP
                     SFASKVEKGDCIVGGRNFGLGSSREHAPTIIKLAGVSAVLAKSFARIFFRNSINVGLP
                     VLICDTDQINQGDELEIDLG"
     gene            complement(417..2699)
                     /locus_tag="AC16GM_02725"
     CDS             complement(417..2699)
                     /locus_tag="AC16GM_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922621.1"
                     /translation="MDLGVPFSQTRTFSERVLSWRAGLALVFVLALVLRLAVYLQSRG
                     LAQMETPILDSRYYLEAGRAIASGAGLPPQPYFMNPGYIWFVAVSSWLFHEPQRPLVV
                     TQIVLDALTCVLAALLAARLFGRLAGLATGFLLAVYGYGILSATRVLPETVAAFLLVV
                     LALMLFRAEDKPRPFMLLFTGVVFGLLTLFRSNALLILPFLGIALWPAKGAARWRTAA
                     MRFSLCLVGAAVVILPVTLHNALSGDDRVLISSSGGVNFYLGNAAGSDGRFVSLNLLP
                     LAPGRFDDDLSGGRFERSVQVYVEEREGRRLKPSEVSAFWMHLARTEITRHPTAWVAL
                     LWRKTYLFFNAFEIPQIDNLYFLAHYLPILGPLAFTSRILWPLGLFGFLLLLRHPGRA
                     RPLFLLFTGYALSIILFFVSARHRLLAAPLIASFAGYGLLRLYDLVRTRPEAGLREGS
                     LRQRWLVPALLFIACLIVTNLNPALGQRVPSGVARGERGSGLFRPGAEYLDFASQHNN
                     LAALLLERGDAVNAERECRQGLVQKPQHPTLLFNLGRALETQGDFSGAREALEQSLRI
                     NPGNAEVAAHLGDMRYKTGDFAGARAALEQAIHLAPLSASAWNSLGPVKYELGDPEGA
                     LAALREAERLAPGWTQPNYNRGLVLSRLGRYGEAIAVLESLYRQGPSNRTFAFALAGA
                     YKGAQRLGAAESLLEQLLAQDPNDTGSLLLLAEIQLTRGETAQARTFLFRVLAANPRN
                     ARALELLRASGSKGKGWVNK"
     gene            complement(2703..4040)
                     /locus_tag="AC16GM_02730"
     CDS             complement(2703..4040)
                     /locus_tag="AC16GM_02730"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR2922622.1"
                     /translation="MGATLAEKVIAGKVSHPVKAGELVITPVDLALAQDGTGPLTIRQ
                     IQKLGVKKVANPNRVIFFLDHSAPASRKELANDHNFIRQFAAQTGVQVSEIGEGISHQ
                     VVAEWHVNPGEVLVGADSHTCTGGALCAFATGMGSTDVAITIALGRTWLRVPETFKIS
                     ICGDFSPGVYPKDLILHLIGLIGADGATYKALEFCGPTIEAMAMHDRLTLSNMAVEAG
                     AKVGMIASDRITHQYLKKMGRPEKWREIRPDKDAVYERTIEIDASKLEPTVSFPHTVD
                     NTHPISEAKGIKVDQVFLGTCTNSRIEDWQIAASICKGRKKHPRVKFIVTPASRWVME
                     EALAKGYYQALLEFGAVITPPGCGPCVGVHAGVPADGEVALTTMNRNFKGRMGNPEAF
                     IYLASPATVVASAITGEITDPRQFVKSGRGKIWKARPAPQTPRKTGKSRRSKK"
     gene            complement(4105..>4618)
                     /locus_tag="AC16GM_02735"
     CDS             complement(4105..>4618)
                     /locus_tag="AC16GM_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR2922623.1"
                     /translation="RIFAVIFIADSIVHLAHLLFSPAGKRLLAGLIPKKRDLAQLVGN
                     LKFYFGLAKTKAPEYLYGYVEKAEYWALVWGGIIMAATGLVLWYKSLFLRFWPKWSID
                     VATTIHFYEAILATSAIFVWHFYAVIFKPGVYPMNWAWLTGRVSGAEIEPEEAAKRET
                     PKPSGEERAS"
BASE COUNT          975 a         1406 c         1338 g          899 t
ORIGIN      
        1 gtcccagatc aatctccagc tcgtctccct ggttgatctg gtccgtatcg cagatcaaaa
       61 ccggcaggcc gacgttgatt gaattgcgga agaaaatccg ggcgaacgat ttggccagaa
      121 cggccgacac gccggccaat ttgatgatgg tcggcgcgtg ctcccgggag gagcccaggc
      181 cgaaattcct gccgccgacg atgcagtcgc ctttctctac tttcgacgca aaggacggat
      241 cggcgtcctc catcacgtgc ttggcgaact cttccaagtt cgagcgcagg tggaagtagc
      301 gtccgggcgc aatcaggtcg gtggagatgt tgtcaccgaa tttccaggct ttgccgataa
      361 gaggcattgt cgttctcctt ctattttcgt ttcaattatt cacaccactc tctgttttac
      421 ttattgaccc atccttttcc ctttgaccct gaggcgcgca agagttccag cgcccgcgca
      481 ttgcgcggat tggcggcgag cacgcggaac aagaacgtgc gtgcctgtgc ggtttcgccc
      541 cgggtcagct gtatttctgc cagtaaaagc agactgcccg tatcattggg atcttgcgcg
      601 agtagttgct ccagaagtga ctctgcagcg cccagccgct gggcaccttt atacgcaccg
      661 gctaaggcaa aagcgaacgt gcgattcgaa ggcccctgcc ggtagagcga ttcgagcacg
      721 gcaatcgcct caccatagcg ccccaagcgg ctcagaacca acccgcgatt gtaattgggc
      781 tgcgtccacc cgggagccag ccgctccgcc tcgcgcaggg cagccagggc accttcggga
      841 tcgcctaatt catatttgac cggcccaagc gagttccacg cactcgccga taagggagcc
      901 agatgaatcg cctgttcgag tgcggcgcgc gcaccggcga agtcaccggt tttgtagcgc
      961 atatccccga gatgagccgc aacctcggca ttgccggggt tgattcgcaa actctgctcc
     1021 agcgcctcac gtgcgccgga aaaatctccc tgggtttcga gcgcccgtcc caggttaaag
     1081 agcaaggtcg gatgctgcgg cttctgcacc aaaccctgcc ggcactcgcg ctcggcgttc
     1141 accgcatccc cccgttccag aagcaaagcc gccagattat tgtgctggct cgcaaaatca
     1201 aggtactctg cgccaggtcg aaataaaccg ctacctcttt cccctcgggc aaccccggag
     1261 ggtacgcgct ggcctaaggc cggattcagg ttggtgacga tcaagcaggc aatgaaaaga
     1321 agggcgggga cgagccaacg ctgtcgaagc gacccttcgc ggagacccgc ttcgggccgg
     1381 gtgcgaacca ggtcgtagag gcgaagcagc ccataaccgg cgaagctggc aataagcggc
     1441 gccgccagca accggtgacg cgccgacacg aagaacaaaa tgattgagag ggcgtagccc
     1501 gtaaagagca agaagagggg gcgcgcccgc ccggggtgcc ggagaagcaa caggaacccg
     1561 aaaagcccca gcggccacaa aattcttgaa gtgaaagcga gcggcccgag tatcgggagg
     1621 tagtgagcaa ggaaatagag gttatcgatc tgcggaatct cgaaggcatt gaagaagagg
     1681 tacgtttttc gccaaaggag cgccacccac gccgtcggat gccgggtgat ttcggttcgg
     1741 gccaaatgca tccagaaagc ggaaacctcg gagggtttca aacgtctgcc ttcccgctct
     1801 tcgacgtaga cctggaccga ccgctcgaac cggccgccgg agagatcgtc atcaaaccgc
     1861 ccgggtgcca ggggcagcag gttcagggaa acgaaacgcc cgtcgctccc cgcggcgttg
     1921 ccgaggtaga aattcacccc gcccgaggag gagatcaaga cgcgatcgtc tccagaaaga
     1981 gcattgtgca gggtaacggg gagtatgact acggcggcac ccaccaggca gagtgagaaa
     2041 cgcatggcgg cagtgcgcca tcttgccgcg ccttttgcgg gccacagggc aattcccaga
     2101 aaaggaagaa tcaaaagagc attgctccgg aaaagtgtca gaagtccgaa caccactccc
     2161 gtgaaaagaa gcatgaaggg acggggttta tcctcagccc ggaaaagcat cagtgccagc
     2221 accaccaaga ggaaagccgc cacggtttcc ggcaggacgc gcgtagcgga gagaattccg
     2281 taaccataaa ccgccaggag aaaaccggtt gccaggccgg ccaaccgccc gaacagccgt
     2341 gccgccaata gcgcggccag aacgcaggtc agggcatcga ggacgatctg tgtcacgacc
     2401 agaggccgtt gcggttcatg aaacagccag ctcgagacgg cgacaaacca gatgtagccc
     2461 gggttcataa aatacggttg cggcggcagc cccgctccgg aggcgatcgc ccggcccgct
     2521 tcgagatagt agcgcgagtc cagaatcgga gtctccattt gcgccagtcc ccggctttgc
     2581 aggtaaacgg ccaggcgcag caccagggcc aagacgaaca ccagtgctaa tccggcgcgc
     2641 caggagagca cccgctcgga aaacgttcgc gtttgtgaaa aaggaactcc taagtccact
     2701 ttttatttct ttgagcgtct cgacttccct gtttttctcg gagtctgcgg tgccggtcgc
     2761 gctttccaga tttttccccg cccggatttc acaaactgcc gcgggtcggt aatttctccg
     2821 gtgatggctg aggcgaccac cgtcgccggt gacgcgaggt aaatgaacgc ctccgggttc
     2881 cccatccggc ctttgaagtt gcggttcatg gtggtgagtg ccacttcccc atcagcgggg
     2941 actcctgcgt gaacgccgac gcacggtccg cacccgggcg gcgtgatcac cgccccgaat
     3001 tccaagagcg cctggtagta ccctttcgcc agcgcctcct ccatcaccca gcgggaagcg
     3061 ggagtcacga tgaatttcac cctcggatgt tttttcctcc ccttgcagat ggaagcggcg
     3121 atctgccagt cttcaatccg tgagttcgtg caggtgccca aaaagacctg atcgactttt
     3181 atgcctttcg cctcgctgat cggatgcgtg ttatcgaccg tgtggggaaa cgacacggtt
     3241 ggttccaatt ttgacgcgtc aatctcaatc gtccgctcgt agaccgcgtc tttatccggc
     3301 ctgatttccc gccatttctc gggccggccc atcttcttca ggtactggtg ggtgatccgg
     3361 tcggaggcga tcattcccac cttcgcgccc gcttccaccg ccatgttcga gagcgtcagg
     3421 cggtcgtgca tcgccatcgc ctcgatggtc gggccacaga actccagcgc tttgtaggtg
     3481 gcgccgtcgg cgccgatcaa gccgatcagg tgcaaaatca aatctttcgg gtagactccc
     3541 ggagaaaaat caccgcaaat ggagattttg aatgtttccg gcacccgcag ccaggtgcgg
     3601 cctaaggcaa tggtgatggc cacgtcggtt gaccccatgc cggtggcaaa agcgcacaga
     3661 gcgccgccgg tacaggtgtg gctgtcggcc ccgactaata cttcgccggg attgacgtgc
     3721 cattccgcca ccacctgatg gctgatccct tcgccgattt cggagacctg cacccccgtt
     3781 tgcgccgcga actggcggat gaagttgtgg tcattggcca gctccttccg cgacgccggt
     3841 gctgagtgat cgaggaaaaa aatcacccgg ttgggattgg ccaccttttt gactcccaat
     3901 ttctggatct gccggatggt gagcggcccg gtgccgtcct gcgccagcgc cagatcgacc
     3961 ggcgtgatga ccagttcgcc cgctttgacc ggatggctca ccttccccgc tatgactttt
     4021 tcggctaacg tcgcacccat gttctcctct cttctatttg aaggcatacg aacccctggc
     4081 cgtcggccgc ggtgtccttc ccgactaact cgcgcgttct tccccgctcg gttttggggt
     4141 ttcgcgtttc gccgcttctt ccggttcgat ttctgctccg gaaaccctcc cggtcagcca
     4201 ggcccagttc atcggataga ctccgggctt aaagataacc gcatagaagt gccagacgaa
     4261 aatggcggaa gtcgccagga tcgcttcgta gaaatgaatc gtcgtcgcca cgtcgatcga
     4321 ccactttggc cagaagcgca ggaatagact cttgtaccag agcaccaggc cggtggcggc
     4381 catgataatg ccgccccaga ccaaagccca gtattcggct ttttccacgt agccgtacag
     4441 gtactccggc gctttcgttt tggccaggcc aaagtagaac ttcagattcc cgaccagctg
     4501 ggctaagtcc cttttcttcg gaatgaggcc ggccagcagg cgttttcccg ccggagaaaa
     4561 cagaaggtgc gccaggtgta cgatgctgtc ggcaatgaat atgacggcga aaatccgg
//
LOCUS       DBNGZD010000103         3957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_781032, whole genome shotgun sequence.
ACCESSION   DBNGZD010000103 DBNGZD010000000
VERSION     DBNGZD010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_781032"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="AC16GM_02740"
     CDS             complement(<1..833)
                     /locus_tag="AC16GM_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR2922624.1"
                     /translation="MRKEASIGIDLGGTAIKSGLVTPTGRVVLELVRKAEVPKGRAHL
                     LGRLRQTITDLLVIAREKKIKVTGIGLGSPGTVNVKTGVVVGNCPNIPHWVGVNLVRE
                     IGRKFRYPFACDNDANLMALAELRLGAARGVKTAICLTLGTGVGGGIVINGEVYRGAW
                     YARAELGHTTIAFDGPRCKCGNYGCLEVFVSAPALVRRFQQSARGRATFGRITPEKIF
                     ALARGGNREARVAVAETGRMLGVGLASFVNAFNPEVIVIGGGVAEGGPLLLQAAKREL
                     RR"
     gene            complement(889..2118)
                     /locus_tag="AC16GM_02745"
     CDS             complement(889..2118)
                     /locus_tag="AC16GM_02745"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR2922625.1"
                     /translation="MDQAAVKEPAQARVPALSPRARVMPPSPIRKLVPLADQAKKKGV
                     KVYHLNIGQPDIPTPEPFLAAVRSFDKKVLEYSNSRGDAGLIEALVTYYRAKGLVVTS
                     EEIQVTTGGSEAILFALLCVTSPGDEVLVFEPFYTNYNGFAVMAGVTLIPVPTSPETG
                     YHLPTREKIEAKISPRTKAILICTPNNPTGTLYTRAEMQLLADLTQEHNLFFISDETY
                     REFAYDGRHVSILEFPGLDGQAILVDSISKRFSACGARVGCLVSRNRQIVNAAWRFGQ
                     ARLSSPTIEMQAAIPLMQLDQNYYDALVSEFKKRRDTVVHLLTKIPGAFCVPPEGAFY
                     SMATLPVDDTEEFARFLLADFSHQGSTTMIAPGSGFYATPGQGKTEARIAYVLNTGDL
                     EKAMQILAKAAEAYQLK"
     gene            2215..2613
                     /locus_tag="AC16GM_02750"
     CDS             2215..2613
                     /locus_tag="AC16GM_02750"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR2922626.1"
                     /translation="MSIKRKSKTRLPTPEEARKLGAIFQSLGDLTRQKILFLLERGPR
                     SVSELVKPFSLTQPTVSRHLAVLKHAGLVTSQRRGQKVIYRLNPEVLRFCHDGFFCCF
                     DCCPVVPLARVMRTAGRGRVTRPIGRGQVA"
     gene            2661..3461
                     /gene="arsM"
                     /locus_tag="AC16GM_02755"
     CDS             2661..3461
                     /gene="arsM"
                     /locus_tag="AC16GM_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR2922627.1"
                     /translation="MNKEETREVVREAYGKIARSQQSCGCGPCGPDATEFAESIGYSE
                     EELKTIPAEANLALSCGNPTALANLKNGEVVLDLGSGAGFDCFVAASKVAPDGKVIGV
                     DMTPEMVEKARDIVRKNGVENVEFRLGEIESLPVEDNSVDVVISNCVVNLSADKPRVF
                     QEVYRVLKPGGKIAISDIALLKELPKKIRESLEAYVGCVGGAILIDEYKKIVEASGLK
                     EVRISIKGSSACIDPTTKDPMGKAILDSLEESESLEGYVVSVYVEGYK"
     gene            3884..>3957
                     /locus_tag="AC16GM_02760"
     CDS             3884..>3957
                     /locus_tag="AC16GM_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR2922628.1"
                     /translation="MGLKIFQVDAFTDSPFTGNPAAVCL"
BASE COUNT          876 a         1130 c         1123 g          828 t
ORIGIN      
        1 cggcgcagtt cccttttcgc cgcctgcaga aggagaggac ccccttccgc cacgccgccg
       61 ccgattacga tcacttccgg attgaaggca ttgacaaaag aagccagccc gacacctaac
      121 atccgtccgg tttcggcaac cgctaccctt gcctcccgat ttcctccgcg cgccagcgca
      181 aaaatctttt ccggagttat gcggccaaac gtggcccgac cccgcgctga ctgctgaaaa
      241 cgccggacta acgccggtgc tgaaacgaag acctccagac aaccgtaatt cccgcacttg
      301 cagcgggggc catcgaaagc aatcgtcgta tgccccaact cggctctggc ataccacgct
      361 ccgcggtaaa cctccccgtt gatgacaatc cctcccccta cgccggtgcc cagcgtgagg
      421 caaatcgcgg tcttgacccc gcgcgcggca cccagacgaa gttcggccaa agccatcaaa
      481 ttcgcatcat tgtcgcaggc gaacgggtag cgaaattttc ggccaatctc ccgcaccaga
      541 ttcaccccca cccagtgggg aatattggga cagttgccca cgaccacgcc ggttttgaca
      601 ttcaccgtcc ccggactccc caggccgatg ccggtaacct taattttttt ctctcgcgca
      661 attaccagga gatcggtgat agtctggcgc agcctgccca agaggtgcgc ccggcctttg
      721 ggcacttcgg ccttgcgaac cagttcgagc acgactctcc ccgtcggcgt caccagtccg
      781 ctcttgatgg ccgtgccgcc gaggtcaatg ccgatggacg cttctttgcg cattagagat
      841 aaaaaaacgg tcgggaaagc accgaccgtt acgccggtga accctgccct atttcaactg
      901 atacgcctcg gccgccttcg ccagaatctg catcgctttc tctaaatcgc cggtattgag
      961 gacataggcg atgcgcgctt cagtctttcc ctggcccggg gtggcgtaga aaccggaccc
     1021 gggcgcaatc atcgtcgtgc tgccctgatg cgagaaatcg gccagaagga agcgggcgaa
     1081 ctcttcggtg tcatccaccg gtagcgtggc catactgtag aaggcccctt ccggcggaac
     1141 gcagaaagct ccgggaattt tcgtgagcag atgcaccacc gtgtcccgcc gcttcttgaa
     1201 ttcactgacg agcgcgtcgt agtagttttg gtccaactgc atcaatggaa tagccgcctg
     1261 catctcgatt gtgggcgaag agagccgcgc ctgaccgaag cgccaggccg cattgacgat
     1321 ctgccggttg cgggaaacca ggcagccgac cctggcgccg cacgccgaaa aacgtttgga
     1381 gatgctgtct actaaaattg cctgaccgtc gagtccgggg aactctaaaa tcgaaacgtg
     1441 gcgcccgtcg taggcgaact cgcggtaggt ctcgtccgaa atgaaaaaga gattgtgctc
     1501 ctgcgttaga tcggccaaaa gctgcatttc cgcccgcgtg tagagcgtgc cggtgggatt
     1561 attgggcgtg cagatgagaa tggccttcgt gcggggagag attttcgctt cgatcttctc
     1621 ccgcgtcggc aggtggtagc ccgtctccgg tgacgtgggc acggggatca gggtgacccc
     1681 cgccattacc gcaaagccgt tatagttggt gtagaacggc tcgaagacca aaacttcatc
     1741 gccgggtgat gtgacacaca aaagcgcaaa caggatcgcc tcgctcccgc cggtggtgac
     1801 ctggatttct tcgctcgtca ccactaaccc tttcgctcgg tagtaggtca cgagggcctc
     1861 gatcaaccct gcatcccccc gggagttgct gtactctaaa acctttttgt caaaactgcg
     1921 gacggcggcc aggaacggct ccggggtggg gatatccggc tgtccgatat tgaggtgata
     1981 gactttaacc cccttctttt tggcctggtc ggctaagggg acgagtttgc ggatgggcga
     2041 aggcggcatc acccgcgcgc ggggcgagag cgccgggacg cgtgcttggg ctggctcttt
     2101 gacggctgcc tgatccacgg aagcagtata gggagggtgc cgctgccttg tcaaaccaat
     2161 tttccaccaa cggcggcgtt tcgctgccgt attaatatat agaatggttt gcatatgtct
     2221 ataaaacgaa aaagcaaaac gcgcctgccc acgccggaag aagcccgcaa attgggagcc
     2281 atcttccagt ccttgggtga tctcacccgg cagaagatcc tgtttttgct ggaacgcggg
     2341 cccaggtcgg tcagcgaact ggtcaagcct ttctccctca cccaaccgac cgtgtcgcgg
     2401 cacctggcgg tgctcaagca cgccggcctg gtgacgagtc aaaggcgcgg ccagaaggtc
     2461 atctaccggc tcaatccgga agtcctgcgc ttctgccacg acggcttctt ctgctgcttc
     2521 gactgctgtc cggtcgtgcc cttggcgcgg gtgatgcgca cagccggcag gggtcgggtc
     2581 acgcgaccca taggcagggg tcaggtcgcc tgaccccaga cgagcagagt tgctatcagg
     2641 caaaaacagg agctgtcaaa atgaacaaag aagaaacacg ggaagtcgtg agagaagctt
     2701 atggcaagat cgctcgaagt caacaaagct gtggctgcgg gccttgtgga ccagatgcca
     2761 cagaattcgc cgaatctatt ggatattcgg aagaagaact gaaaaccatc ccagctgagg
     2821 ccaatctggc tcttagctgc ggaaatccga ccgccttagc caatctcaag aacggtgagg
     2881 tggtacttga ccttggttcc ggtgccggtt ttgattgctt tgtagctgca tcgaaggtag
     2941 ctccggatgg taaagtcatc ggtgtggaca tgacaccgga gatggttgag aaagcaagag
     3001 acattgtcag aaagaacgga gttgaaaatg ttgaattcag gcttggcgag attgagagct
     3061 tacccgtgga agacaattcg gtggatgtgg tcataagcaa ctgcgtggtc aacctctcag
     3121 ccgataagcc gagagtcttc caagaggtct atagggtctt aaagccaggg gggaaaatag
     3181 cgatttctga tattgccctt ctcaaggaac ttcccaagaa gattcgggag agcctggaag
     3241 cttatgtggg ttgtgtagga ggggcaatac ttatcgacga gtacaaaaag atcgttgagg
     3301 cgtctggttt gaaggaggta agaatttcga tcaaaggctc ttctgcctgt atcgatccca
     3361 cgaccaaaga cccaatgggt aaggccattc tagatagctt ggaagagagt gagtctctcg
     3421 aaggctatgt ggttagcgtc tatgtggagg gttataagta agtggcagca attttttgcc
     3481 taaccattcg ttccggcgga cgtggtaaac cgcgcccccc aactttgtcg atcgaacgac
     3541 ccgaatgcct gacccgcaaa aggaggtgat acggatgctg cgtcgccgct ggatgctccg
     3601 cgcggtctgc aactgcccct gcggttgcca tagccggtcc tgagcacgac gctaaactgc
     3661 tcgaccaacc ccggatcggt gtgaatgtcc gatcccgatc tccgaccggt cgaatttcaa
     3721 ccccgccctc tttggcgggg ttttttatgg tacacccttt aatcaaaact gcgtagaagc
     3781 atctctggtc gtagccgccc taaatgaacc ccgaacccct ttgacacgcg acaaggccgt
     3841 agaatagttt cccgtaccgg tagttcagaa acgggaggaa cagatgggat tgaaaatttt
     3901 tcaggttgac gctttcaccg acagtccctt caccggcaat ccggcagcgg tctgcct
//
LOCUS       DBNGZD010000104         9455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_850871, whole genome shotgun sequence.
ACCESSION   DBNGZD010000104 DBNGZD010000000
VERSION     DBNGZD010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_850871"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16GM_02765"
     CDS             complement(<1..245)
                     /locus_tag="AC16GM_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922629.1"
                     /translation="MGLKEILIGVVSVIIGALLSGIGYLVKTRYEHKKLLNEALFNLL
                     EIWYVIRISTFFDVSDYMETYLRRVKDRFPEANFTKE"
     gene            complement(279..1814)
                     /locus_tag="AC16GM_02770"
     CDS             complement(279..1814)
                     /locus_tag="AC16GM_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR2922630.1"
                     /translation="MSPGERKILLISGDEILRRSLSDAFAKAGLTLSAAAQAQEGLKL
                     LREESFPLILLDSRPIGLEPEKLVRKIISKNPLADLVALTPAGMPDLRARLSDLGIND
                     FFDLATPPDEIVFHSRELLLQRQLLEQVGFVGKSPEIKRIAETLPQVAASDLVALITG
                     ESGTGKELLAQAIHKSSARKDKPFLAINCGAIAEGVLESELFGHEKGAFTGAIAQRPG
                     MFEVAHGGTLLLDEIGEMKPATQVKLLRVLEEKSFLRVGGKAKITVDVRVIAATNRDL
                     AAEVASGNFRRDLYYRLSVIKLDLPPLRARRGDIPLLFFYFINQLSASGHTARMGGRP
                     LEVTDEALRLLSDYHWPGNVRELKNFVERAGVLSPDGRITGREVQEYIESQSALGGLN
                     RMLPVATGKSSETVERELLYQALVGLGRQVNELRQLLISALGGGSSPLAVRTSPPQAD
                     WGNLNPSQEIIQPVTLDDVEREAIERALTEVHGNRKKAAQLLGIGERTLYRKIDKYGL
                     RKR"
     gene            complement(1819..4002)
                     /gene="rnr"
                     /locus_tag="AC16GM_02775"
     CDS             complement(1819..4002)
                     /gene="rnr"
                     /locus_tag="AC16GM_02775"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR2922631.1"
                     /translation="MSEIKTLDKNRIIEYLKKSGYQPAKTRRLAREMAIPEDLYGQFR
                     RLVRELLRAGEIVELKHNRLALPDKEKFVVGRIYGHRNGYAFLGPEKGGDDIFINPQN
                     LKTALHGDKVLATLVASRTGRSVEGRVVKILERGKKTVVGTFHHSRLADFVVPDDSRF
                     PKEIYIPPGATDGARDCQKVVVTLAPWEDEYRKPEGKISEILGYPGDPGLEILTIIRE
                     FDLPHEFPQEVEHEAAKIPEKIPAAEIKRRLDWRKKITFTIDPVDAKDHDDACSIEKL
                     ANGHWLLGVHIADVSYYVTEGSNADREALLRGTSVYLADRVIPMLPERLSNNLCSLVP
                     KKDRLTFSCLLEIDRHGKVHKSEVAESIIESRAKLAYEQVQAFFDTGKASPEIKGLEA
                     SLTEMRALARILLENRKKRGSLDFDLPEPHVLLDKEGRVIDVYQKIRLESHRLIEEFM
                     LLANQTVAELFTAKNFPILYRVHDRPAPEKMESFAAMMADFGYALHFKDEPTPFRLAR
                     FLEQVRGRPEEELVNELLLRSLKKAVYQPENIGHFGLAFDHYLHFTSPIRRYPDLLVH
                     RLGKELLRGRPTVSRFRWLEANLSKIGQITSERERVAEEAERESVKVKQLEFMQHKLG
                     EVYPGIVSGVTSFGVFVRIVALGVEGLVRFSALGDDYYIFEEEKHRLVGRRSKKVIRL
                     ADPMKVQVLRVDLLQKRLDLVPFGEEERPTAPTEKRPPQRGRRHR"
     gene            complement(4199..4714)
                     /locus_tag="AC16GM_02780"
     CDS             complement(4199..4714)
                     /locus_tag="AC16GM_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR2922632.1"
                     /translation="MTRKIFTGAATLVALGLLAGGAFGQPKKKERAGDQKKMEGMKLS
                     DEQKAKLQEMHFTFAKEQTMLRAKERVAALELRQMLSEDQPSREALMHKWGEVREYKN
                     QLAQRRFQELLSVRDVLTKDQREAMRHKMGEKFMQRRMHGRPGFMGPGMHRPGQMPQM
                     PMAPPEPPEPE"
     gene            complement(4740..5252)
                     /locus_tag="AC16GM_02785"
     CDS             complement(4740..5252)
                     /locus_tag="AC16GM_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922633.1"
                     /translation="MKTAIARRAEGITIGALVLALVVGGSATAQMRRRPPRPPEERQK
                     WQERLATLRELKLIEVLELSDEQSNQFLPAFKAYQKKRGLVAGKRDVVLQELGLLLEE
                     EGTSDQKLQEKIDQLGELRKEQNQLDNEFFAKARTILKPRQMARMAVFEVRFERAILE
                     QLRQRSEEQP"
     gene            complement(5270..5866)
                     /locus_tag="AC16GM_02790"
     CDS             complement(5270..5866)
                     /locus_tag="AC16GM_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR2922634.1"
                     /translation="MKCGDVKNYLTDYARGALAVGLREQVSLHLEGCPACHALLAEEE
                     RLAQLLAAEQPAEPSDTFWANFLPRVRERIAVRKKKRKSYLVRRWVYVTGSVAAILVL
                     SLFAHQRWFGQNEPTGWLSQLPPGVTVEDVVGYVAARGGDGQLASAAIGADSVYIAGL
                     VSAAQESLAQEGRLELVFHQIPEGEALRVLAEVERLKL"
     gene            complement(5863..6474)
                     /locus_tag="AC16GM_02795"
     CDS             complement(5863..6474)
                     /locus_tag="AC16GM_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2922635.1"
                     /translation="MEDARAREQSDPAKGGAHHELVSRFQSGDERAFNEIVLLYQKPV
                     YLVAFRMVRNHQDAQDLAQEVFVRTYSGLKKFKYEASLFTWLYRVTVNLAINFARRER
                     FKQFVSIFDVGELLTSKDDPRIDFQRDLLSRQIDGAVLKLPPRQRAVFVLRHYHDLPH
                     AEIAKIIGRTEGAVKANYFQALAKLKKFLRPESLRPVENVSAV"
     gene            complement(6602..8140)
                     /locus_tag="AC16GM_02800"
     CDS             complement(6602..8140)
                     /locus_tag="AC16GM_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR2922636.1"
                     /translation="MLTGWRRGFLVGWGLLAALVGTPSGSFAADKNITLLAQRAATNP
                     TGGYGPGYSGCWGYGDSLGREFALMCGYFELDIWDVTNPANPILAKTIPTTGSDLKQV
                     RPYRHYLYAVNQSGPLQIIDVSDPYNASTVAGYLPCAAGAGSHTIHIDGHYAYLNTAG
                     SCARDLRILDLTDPLNPVEVGHYTYPGPGFVDAHDSYVYGNTCYVANLAGGVSILDVS
                     NKTQPVLQAIITYPGNFDHNCWTTTDGKYLLTTDETCQGHLRVWDVRDLSNIREVGSY
                     AAGPNHIIHNVQVNGVWAFISYYSEGVKVVDIEDPTDPIEVGSYDTYPQGSTCSYGGC
                     WDVFPYFPSGTLVASDMANGMFLLRFNGARAGRIEGRVFNVGNGQSVAGATVRLLEIG
                     KSFVTGTDGRYAFRTGDGLRTIEVSRPGFRTDTVQVTAVLSDTISADLPLTPTLQGDF
                     NGDLEVNLADVVVLINAVVFNIPPLAPQAADLTGDCLVDLVDIITLINFVVFNNVSLQ
                     SCPP"
     gene            complement(8412..8825)
                     /locus_tag="AC16GM_02805"
     CDS             complement(8412..8825)
                     /locus_tag="AC16GM_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR2922637.1"
                     /translation="MKSHTEYLWFNTKKHREFINITGEVETALGKSGIKEGFALVSAM
                     HITASVYVNDAESGLIADIEEWLEKLAPSGVNYRHHRTGETNGDAHLKNLLMHHEVIV
                     PVTAGKLDFGPWQQIYYAEFDGQRKKRVVIKIIGD"
     gene            complement(8868..9338)
                     /locus_tag="AC16GM_02810"
     CDS             complement(8868..9338)
                     /locus_tag="AC16GM_02810"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2922638.1"
                     /translation="MSPQELTAYFDEIYALNLACLDLVPEEKLDFQPVPEVKSLRLLL
                     HHMYVNQKFYLLTARKGTMDVDDYKAMMKQVPDTKESLRREMGETFAEFKQALGDEAL
                     LKKEVQTLSGSRRVYHLFIGELEHQLHHRGQVYTYLRLLGLKPPDSGLYMGLAS"
BASE COUNT         1906 a         2736 c         2723 g         2090 t
ORIGIN      
        1 tcttccttgg taaagttagc ttcaggaaag cggtccttaa ctctacgaag ataagtttcc
       61 atataatcac tgacatcaaa aaaagtggaa attcgaataa cataccaaat ctctagaaga
      121 ttgaacagtg cttcattcag aagttttttg tgctcgtacc tagtcttaac tagatagcca
      181 atcccgctta ataacgcccc gattattaca cttactacac ctattaaaat ttcttttaag
      241 cccatttaaa atagtacctc gtgctctttt gtatttcctc agcgtttcct caacccatac
      301 ttatctatct tcctatacag cgtcctttcg ccaatgccca aaagttgcgc ggctttcttg
      361 cggttgccgt gcacttcggt gagggcgcgc tcaatggcct cgcgctccac gtcgtccaag
      421 gtcacgggct gaatgatttc ctgagaggga ttgaggtttc cccaatccgc ctgaggcgga
      481 ctcgtcctta cggcgagcgg gcttgagcca ccgcccaggg cggaaataag caactgtcta
      541 agttcattca cctgccggcc cagccccact aacgcctggt agaggagttc ccgctcgacg
      601 gtttcggaac ttttgcccgt cgccacgggc agcatgcggt tcaatccacc taaggcggac
      661 tggctttcga tatactcctg cacctcccgc ccggtgatgc ggccgtcggg agagagcacc
      721 ccggcccgct ccacgaaatt cttcaattcc cgcacgttcc ccggccagtg gtagtcggaa
      781 aggaggcgca acgcttcgtc ggtgacctcc agcgggcgcc cgcccatacg ggccgtatgg
      841 cctgaggcgg agagctgatt gatgaagtag aagaagagca gcggaatgtc gccccggcgc
      901 gcgcgcaagg gcggcaggtc gagtttgatg accgagagcc ggtagtagag gtcgcggcgg
      961 aaattgccgg aggcgacttc ggcggccagg tcgcggttgg tggcagcaat cacccgcacg
     1021 tcaacggtga ttttcgcctt cccccccacc cgcaggaacg atttctcttc caacacccgg
     1081 aggagtttta cctgcgtggc gggcttcatt tcgccgattt cgtccaagag gagcgtgccg
     1141 ccgtgggcga cttcgaacat tccgggccgc tgggcgatgg cgccggtgaa ggcgcctttc
     1201 tcatggccga agagttcact ctctaacact ccctcggcga tagcgccaca gttgatggca
     1261 aggaacggtt tatctttccg cgccgaactt ttgtgaatcg cctgcgcaag gagttccttg
     1321 cctgtgcccg attcgccggt gatcaaggcg accaggtcgc tcgcggccac ctgaggcagc
     1381 gtttcggcaa tgcgtttgat ctccggcgac ttgccgacaa agccgacctg ttccaaaagt
     1441 tgccgctgca acagcagttc acggctgtgg aagacgattt catcaggcgg cgtggcaagg
     1501 tcaaagaaat cgttaatgcc gagatcggaa agccgcgcgc gcaggtcggg cattccggcg
     1561 ggggtgagag cgaccagatc ggctaacgga tttttgctga taattttacg gactaatttt
     1621 tccggctcca agccgatggg ccggctgtcc aacagtatga gtggaaacga ctcttcgcgg
     1681 aggagtttca atccctcctg cgcctgcgcc gcggcagaga gggttaaacc ggctttggca
     1741 aaagcgtcgc tgagcgaacg gcggagaatt tcgtcgccgg agatgaggag aatttttctt
     1801 tcgcccggag acataacctc acctgtgccg ccgcccgcgc tgcggaggac gtttctcggt
     1861 gggagcagtc ggccgctcct cctcgccgaa aggaactaaa tcgagacgct tttgcaggag
     1921 gtcaacgcgg agcacctgca ccttcattgg atcagcgaga cggatgacct tcttggagcg
     1981 ccgccccacc agccggtgct tttcttcttc gaagatgtaa tagtcatctc ccagagcgga
     2041 gaaacggacg agcccttcaa cgcccagggc cacgatgcgg acgaaaaccc cgaagctggt
     2101 gacgcccgaa acgatgccgg gataaacctc gcccaatttg tgctgcatga attccaattg
     2161 cttgactttg accgattccc gctcggcttc ctcggcaacc cgctcccttt cggaagtgat
     2221 ctggccgatt ttgctcaggt tggcctccag ccagcggaag cgggacactg tcggacggcc
     2281 acggagaagt tccttgccga ggcggtggac caagagatcg ggatagcgcc ggatggggga
     2341 ggtgaagtgc aggtagtggt caaaggccag gccgaagtgg ccgatattct ccggctggta
     2401 gaccgctttt ttgagggagc ggagcaagag ttcgttgacc aactcctcct cgggccggcc
     2461 gcgcacctgt tccaaaaagc gggcgagacg gaacggcgtc ggttcatctt tgaaatggag
     2521 cgcgtaaccg aaatcggcca tcatcgcggc aaacgattcc attttttcgg gagcgggtcg
     2581 gtcgtgcacg cggtagagaa tcgggaaatt ttttgcggtg aaaagttccg ccaccgtttg
     2641 attggccaaa agcatgaact cttcaatcag ccggtggctt tccagccgga ttttctggta
     2701 gacgtcgatc acccggccct ctttgtccag gaggacgtgc ggctcgggga ggtcgaaatc
     2761 gagactgccg cgcttcttgc ggttttcgag aagaatgcgg gccaaagcgc gcatctcggt
     2821 gagactggct tcgagtcctt tgatttcggg tgaggccttg ccggtgtcga aaaacgcctg
     2881 cacctgctcg taggccagct tcgcccggct ctcgatgatc gattcggcca cctccgattt
     2941 gtggactttg ccgtgccggt cgatttcaag aagacaggag aaggtcaggc gatctttctt
     3001 cggcaccaga ctgcaaaggt tgttcgagag ccgctccggc agcatcggaa tcacccggtc
     3061 ggccaaatag acgctggttc cgcgcaggag cgcctcgcgg tcggcgttcg agccctcggt
     3121 gacgtagtac gaaacgtcgg cgatgtggac gcccaagagc cagtggccgt tggccagttt
     3181 ttcgatggag caggcgtcgt cgtggtcttt ggcgtcgacc ggatcgatgg tgaaggtgat
     3241 ttttttgcgc caatcgagcc ggcgcttgat ttcggcggcc ggaattttct ccgggatttt
     3301 cgccgcctcg tgctccactt cttgcgggaa ctcgtgcggg agatcgaact cccggatgat
     3361 ggtgaggatt tcgagaccgg gatcgccggg gtagcccagg atttctgaaa tctttccttc
     3421 cggtttgcgg tattcatctt cccagggggc gagcgtgacg accactttct ggcagtcgcg
     3481 ggcgccgtcg gtcgctccgg gcgggatgta aatttctttc gggaagcggg aatcgtcggg
     3541 tacgacgaag tcggcgaggc ggctgtggtg gaaggtgcct accacggtct tcttcccgcg
     3601 ctcgaggatt ttgaccaccc gtccttccac acttctgcct gttcgggaag caaccaatgt
     3661 cgccaagact ttgtcgccgt gcagggcggt cttgagattc tggggattga tgaagatgtc
     3721 atccccgccc ttctccggcc ccagaaaggc gtagccgttc cggtgcccgt aaatccggcc
     3781 gacgacgaac ttctctttgt ccggcaaagc cagccggttg tgcttcaact cgacaatctc
     3841 cccagcgcgg agtaattcac gcaccaaccg ccggaactgg ccgtacagat cttccggaat
     3901 agccatttcc cgggctaatc tgcgagtttt ggctggctgg taaccggact ttttaaggta
     3961 ttctatgatc cggttcttgt ctaaagtttt aatttctgac attttggcag aaaataagtc
     4021 aaaaagaacg tttagtcaaa gaggttttta actgattgtc gggctcgtcc ccgagcccga
     4081 caatccggct ctggtcggca cgactcgtac gaggacgagc cggactacca ccgaggtgcc
     4141 tgttcaatca aatgaaaagc cccgccaatc ggtggggctt tttcccagag gagggtggtc
     4201 actccggttc gggtggctcc ggaggagcca tgggcatttg tggcatttgg cccgggcggt
     4261 gcattccggg gcccataaat ccgggtcggc catgcatccg gcgctgcatg aatttttccc
     4321 ccatcttgtg gcgcatcgct tcgcgctggt ctttggtcag cacgtctcgc actgacaaga
     4381 gttcctggaa gcggcgctgg gcgagctggt ttttgtactc ccggacttca ccccatttgt
     4441 gcatcagcgc ttcacgactg ggctggtctt cggagagcat ctggcgcagt tccaatgcgg
     4501 ccacccgctc tttggcccgg agcatcgtct gctccttggc gaaggtgaag tgcatctctt
     4561 gcagcttcgc cttctgttcg tcggagagtt tcatcccctc cattttcttc tggtcgcccg
     4621 cgcgttcttt tttcttcggc tgaccgaagg cgccgcctgc cagaagtccc agggccacga
     4681 gcgtggctgc tccggtgaag atcttcctgg tcatctttct caccatcctt tcattatctt
     4741 tagggttgct cttcggatcg ctgtcgcagc tgttctaaaa tggcccgctc aaagcgcacc
     4801 tcgaagaccg ccatccgcgc catctggcgc ggtttgagga tggtgcgcgc tttggcgaaa
     4861 aactcgttgt cgagctgatt ctgctctttc cgcaactcgc ctaattggtc gattttctcc
     4921 tgcagtttct ggtcggaggt accctcttct tccaagagca gacccagttc ctgcaacacc
     4981 acgtcgcgct tgccggcgac caaaccccgc ttcttctggt aggccttgaa ggccgggagg
     5041 aattgattgg actgctcgtc ggagagttcc agcacttcga tcagttttaa ttcgcgcaaa
     5101 gtcgccagcc gttcctgcca cttctgacgc tcctccgggg gacgcggtgg ccggcggcgc
     5161 atctgggcgg tggcgctgcc acccaccacc agtgccagca ccaaagcgcc gatggtgatg
     5221 ccttctgctc gtctcgctat cgcagttttc atcgtaaacc tgcccttttt cagagtttca
     5281 aacgctccac ttccgccagc acccgcaggg cttccccctc gggaatttga tggaagacta
     5341 attccagacg cccctcctgc gccagcgact cctgagcggc agacaccagc ccggcgatgt
     5401 agacgctgtc ggcgccgatg gcggcacttg ccagttgtcc atcccctccc cgcgcggcca
     5461 cgtagccgac gacgtcctcg acggtaaccc ctggcggcag ctggcttagc cagccggttg
     5521 gttcattctg cccgaaccac cgctgatggg caaaaaggct gagcaccaga atagcggcga
     5581 cgctgccggt gacatagacc caacgacgaa cgagataact ttttcttttc tttttccgga
     5641 cggcgatccg ttcccgcacc cggggcagga agttggccca gaaggtgtcg gagggctcgg
     5701 cgggctgttc ggcggctaag agctgcgcca agcgctcttc ttccgccagg agcgcgtgac
     5761 aggccgggca accttccagg tgaagagaaa cctgctcgcg caaaccgacg gcgagggcgc
     5821 cgcgagcgta atcggtcaga tagtttttaa cgtctccaca tttcataccg cactcacgtt
     5881 ttccaccggt cggagtgatt ccggcctcaa aaactttttc aatttcgcca gcgcctggaa
     5941 gtagttcgct ttgaccgcgc cttcggtccg gccgatgatt ttggcaatct ccgcatgggg
     6001 cagatcgtga tagtgcctca ggacgaagac cgctcgctgc cgcggcggca gtttgagcac
     6061 cgccccatca atctggcgcg aaagaaggtc gcgctgaaag tcaatccgag gatcgtcttt
     6121 gctcgtcagc agctcaccga cgtcgaaaat cgacacgaac tgtttgaacc gctcgcgccg
     6181 ggcgaagttg atggcgagat tcaccgtcac ccggtagagc caggtgaaaa gcgaggcttc
     6241 gtacttgaac tttttcaacc cggagtaagt ccgcacgaac acctcctgcg ccaagtcctg
     6301 cgcgtcctgg tggttgcgca ccatccggaa ggccaccaga tacaccggct tctggtagag
     6361 gaggacaatt tcgttgaaag cccgctcatc gccggactga aaccggctga caagttcgtg
     6421 gtgcgccccg cctttggcgg ggtcggattg ctcccgcgcc ctggcatcct ccatctcacc
     6481 gcctgctatc gtattagacc ggtcaccctg ggaatagttt aaccctcaga gcggggcttg
     6541 ccaaacctcc gccaaaagaa aacccccggt caaaaccggg ggtctctctc cttgtcatcc
     6601 ctcagggagg gcaggactgc agggaaacgt tattgaatac gacaaagttg ataagcgtga
     6661 tgatgtccac cagatcgacc aagcagtcgc cggtcaggtc agccgcctga ggtgcaagcg
     6721 gggggatatt gaaaaccacg gcattgatca gaactaccac atcggcaaga tttacctcca
     6781 ggtcaccatt gaagtccccc tgcagagttg gggtgagggg caagtcggca ctaatggtgt
     6841 cactcaaaac cgccgtgacc tgaacagtgt cggtacgaaa acccggtcga ctcacttcga
     6901 ttgtgcgcag gccgtcaccg gtgcggaagg cgtagcgccc atcggtcccg gtcacgaaac
     6961 ttttgccgat ttccagaagg cggacggtag cgccggccac cgattggcca ttccctacgt
     7021 tgaacaccct gccttcaatt cgacctgcgc gcgccccgtt gaagcgcaag aggaacatcc
     7081 cgttggccat gtcgctcgcg accagcgtgc ccgatggaaa ataggggaac acgtcccagc
     7141 agccgccata actgcaggtt gaaccctggg gatacgtgtc atacgaacca acttcgatcg
     7201 ggtcagtggg gtcttcgata tccaccacct tgaccccttc ggaatagtac gaaatgaagg
     7261 cccacacccc gtttacctgg acgttgtgaa tgatgtggtt cggtccggcc gcgtagctcc
     7321 ccacctcccg gatgttgctg agatcgcgca cgtcccagac ccgcaggtgg ccctggcagg
     7381 tctcatcggt ggtcaaaagg tacttcccat ccgtcgtcgt ccagcagttg tggtcaaagt
     7441 tccccggata agtaattatc gcctgcagca ccggctgcgt cttattgctt acgtccagga
     7501 tactcacccc gcccgccagg ttcgccacgt agcaggtatt gccgtaaacg taggagtcgt
     7561 gcgcatccac aaagccgggg ccgggatagg tgtagtgccc cacttcgact ggattgagtg
     7621 ggtcggtgag atcaaggatg cgcaggtcgc gggcgcagga cccggccgtg ttgaggtagg
     7681 cgtagtggcc gtcgatgtga atggtgtggg agccggctcc agcggcgcag gggaggtagc
     7741 cggcgacggt gctggcattg tatggatcag acacgtcaat tatttgcagc ggcccgctct
     7801 ggttcacggc atagaggtag tggcgatacg ggcgaacctg tttgagatct gagccggtgg
     7861 tcggaatagt tttggccagg atcggattgg ctggattggt gacatcccag atgtcgagtt
     7921 caaagtagcc gcacattaaa gcgaactcac ggcctaaact atccccatag ccccagcagc
     7981 cggagtagcc gggaccgtag ccgccagtgg gattcgtggc cgcgcgctgg gccaaaaggg
     8041 tgatgttctt atcggcagcg aaacttccgg acggcgtccc aacgagagcc gccaaaagcc
     8101 cccatccgac caaaaatccc ctgcgccatc ccgtcagcat ttgcctctca cgaccggatt
     8161 aaaacctttc acatcttaac cgcaccactg acccgcaaaa agaaaggccc tcccgcaccg
     8221 gcgggattgc ccctctccag acagagtata tagaaaagaa cacaaaagtc aaccggtttg
     8281 tgcccggtcc attcggaatc aggatttggg agtcaggaat tagggggaga aaaaagtccc
     8341 tcccctttga ggggagggat gcagggaggg gtcgtagctt agccccttgt gggcgtggcc
     8401 gcaaagaatc gctaatcacc aataattttt atgactaccc gcttcttgcg ttggccgtcg
     8461 aactcggcgt agtaaatctg ctgccagggg ccaaaatcga gtttgccggc ggtgaccggg
     8521 acgatgacct cgtggtgcat caggagattt ttcaggtgcg cgtcgccgtt ggtttcgccg
     8581 gtacggtggt gccggtagtt gaccccggac ggggctaact tctccagcca ttcttcgatg
     8641 tcggcgatta agcccgattc tgcgtcgttg acgtagaccg aggcggtgat gtgcatcgcg
     8701 gagactaatg cgaagccctc tttaatgccg cttttaccca gcgctgtctc cacctcaccg
     8761 gtgatgttga tgaactcccg atgcttcttc gtgttgaacc agaggtattc agtgtgggat
     8821 ttcatagtga gaaattagcg ggtcgatccg catgaaccaa ttattactta gctcgctaat
     8881 cccatataca acccgctatc tggcggtttg aggcccaaga gccgcaggta ggtgtagacc
     8941 tgccctctgt ggtgcagttg gtgctctaat tcaccaatga acaggtggta gacacggcgc
     9001 gagccggata aggtctggac ctcttttttg agcagggctt cgtcgcccag cgcctgctta
     9061 aattcagcga acgtttcccc catttcccgc cgcagcgatt ctttggtatc cggcacctgc
     9121 ttcatcatcg ctttgtagtc gtcaacgtcc attgtccctt tccgggcggt aaggaggtag
     9181 aacttctggt tcacgtacat atggtggagc aagaggcgca gcgatttcac ttccggcacc
     9241 ggctggaaat caagtttctc ttccggcact aaatcgaggc aggccaggtt gagcgcgtaa
     9301 atttcgtcga agtaggcggt taattcctgc ggagacatag aagaaaaatg caggatgaac
     9361 gggcaaaaga caagacccac gccctgtcgg gcgccgagga taaaatcctc ggctacgacc
     9421 tctccccaac cctctcctca aaggagaggg agaaa
//
LOCUS       DBNGZD010000105         1922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1545425, whole genome shotgun sequence.
ACCESSION   DBNGZD010000105 DBNGZD010000000
VERSION     DBNGZD010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1545425"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="AC16GM_02815"
     CDS             <1..1380
                     /locus_tag="AC16GM_02815"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR2922639.1"
                     /translation="FLVIFLGVFAVGAAGLLLWRSRQIKSERLVKNLLAKEFSVFLSL
                     VLLVVTTIIVLLGTSAPLLTRIWGQPGNVNTVYYVRTSLPIGILLAVVLGVMPYLLWR
                     GSGLQVLALRALWPAVAGLAIVGLSILLGFRSPLYILFGFFAVFALLSNFLVFYRTFQ
                     LKPAYVGGHLTHVGVAFMLLGLITSNVYSTSEKITVTRGESKKAFGYQFTYQGLAGPV
                     EEKDNAVHVLVTRGQKSFTATPKFFWSEYNRDFMRKPYIKRAVFSDLYISPMAHSVMD
                     RAGQNTLALKQGETKEAYGYKITFENFEVLEHDQEKGLIKVGAVLSVAPYGRKAVVGS
                     PGPQRREPVFVLVGEEQSLIPDTLPGGELIYLAAPKPETKEVVLGFSGLNQAQAAAAD
                     YVVLDISRKPLINLLWIGTTCVALGALTASYRWGRRRMGTKELSVRQVGQAPPLSAEV
                     KEPTLATRV"
BASE COUNT          369 a          567 c          553 g          433 t
ORIGIN      
        1 tttctggtta ttttcctggg agtcttcgcc gtcggcgccg ccggacttct cctctggcgc
       61 agccggcaga tcaaaagcga gcgcctggtc aaaaaccttt tggctaaaga attttccgtg
      121 ttcctctcac tggtactttt ggtcgtcacg acgatcatcg tgcttctggg cacctccgcg
      181 ccacttctga cccgcatctg gggccagccg ggcaacgtca acaccgtcta ctacgtgcgg
      241 accagtttgc ccatcggcat tcttttggcc gtggtcttag gcgtcatgcc gtacctttta
      301 tggcggggtt cggggctcca ggttctcgcc ctgcgggcgc tctggcccgc ggtcgccggg
      361 ctagccattg tcggcctttc catccttctg gggtttcgtt cgcctttgta catcctcttc
      421 gggttttttg ccgtctttgc gctcctgtcg aactttttag ttttctaccg tacgttccag
      481 ctcaagccgg cctacgtggg cggacatctg acccacgtgg gggtggcctt tatgctcctg
      541 ggcctcatta cctcaaacgt ctactccacc tccgagaaaa tcaccgtgac ccggggggag
      601 tcgaagaagg cgttcgggta ccagttcacc taccaggggc tggccgggcc ggtggaagaa
      661 aaagataatg cggtacatgt gctggtcacg cggggacaaa agtcatttac cgccacgccc
      721 aagtttttct ggagcgaata caaccgcgac ttcatgcgca aaccgtacat caagcgggcc
      781 gtgttttccg acctctacat ctcgcctatg gctcactcgg tgatggatcg agccggacag
      841 aacacgctgg ccctgaagca gggagaaacc aaagaggcct acgggtacaa aatcactttc
      901 gagaatttcg aagtcctgga gcatgaccag gaaaaaggct tgatcaaagt cggcgcggta
      961 ctgagcgtgg ccccctacgg ccgcaaggcg gtcgtcggca gccccggccc ccagcgtcgg
     1021 gaaccggttt ttgttctggt cggggaggag cagagtttga ttcccgacac gctgccgggc
     1081 ggtgagctca tctacctggc cgcgccgaaa ccggagacca aggaggtcgt attgggtttc
     1141 agcgggttga accaagccca ggccgcagcg gccgactacg tggtgctcga catttcgcgc
     1201 aaaccgctca tcaacctcct ctggatcggc accacctgcg tcgctctggg tgcgctcacg
     1261 gcgtcgtacc gctggggtcg gcgcaggatg gggacgaaag agctgagcgt acgccaggtc
     1321 ggtcaggcgc cacccctatc cgcagaagtt aaagagccaa ccctggcaac cagagtatag
     1381 acattctttt atcgaaaaag aaagtgaagc gcagggaaag aacccgcgct tctttttttg
     1441 cattacccct gccgagcccc gacgctcttc ctttttgggt gagggtggtt tgatgtcggc
     1501 tgactctgga gagcaggcgt tagcgccgca cctcaccccc ggactgacgc gcgaacgccc
     1561 gctccggtct cccggtttgc ctttccgccg cctcttttgt ataattggga acgcgaggca
     1621 tgaatctgga aatcgcaaaa aaatctgcac ggtagcttcg gaggctttcc gggaagtcga
     1681 gtcggcgtcg gtctgctttg tgtggcgcgc tttgcgccgc ccgggtgcaa cctacgattt
     1741 tggtcggatt gtgccgattc atagagagta gagaagaccg cttctcagtc tggtaaaacg
     1801 tacgacactg ccatgcccga ttttgaatca ccgcttgagc acttggactc ggttgatcgg
     1861 ctgcggagca cgattgaagc caaaatctcc gaactgtccg aggccaacaa gctccacaaa
     1921 cg
//
LOCUS       DBNGZD010000106         4031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_87219, whole genome shotgun sequence.
ACCESSION   DBNGZD010000106 DBNGZD010000000
VERSION     DBNGZD010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_87219"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="AC16GM_02820"
     CDS             <1..383
                     /locus_tag="AC16GM_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922640.1"
                     /translation="DSGLVEAYVNWGLVLNKIGQHDSAIEKYRTALTRKPDFGEAYKA
                     YFNWGLALIGLGRHNEAVEKFEKAVQKKPDYADAHYNLGVALTKGNRKEEAIKELSKA
                     RELSIKQGKMELVLKADKLLNRLK"
     gene            422..2149
                     /locus_tag="AC16GM_02825"
     CDS             422..2149
                     /locus_tag="AC16GM_02825"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR2922641.1"
                     /translation="MLKYTLVFFLALVSLTFAQTTSPLKDAKIHLDFKHYYDYAELTQ
                     VLHDLASAYPQFTHLESMGKSLKGRDLWVLTVGNPKTGDLAKKPAMYIDGNTHGNEIQ
                     GGEVALYTIYYLLTRYGKDDYITKLLDTRMFYIAPSVNPDARDAFIHQPNNPHMPRQN
                     YRPWDEDHDGLVDEDGPEDIDGDGRILSMRVKNPLGNWKKGDDPRDMRPRQPDEPGEY
                     DFYFTEGIDNDGDGLINEDEAGGVDLNRNFPAAWQPRYQQYGAGDYPLSEPETRSTIE
                     WIVTHPNIAAIQSYHNAGNLILRPFGFKDDKGFPSRDLAMYRAMQRRGEAFLPGYKAW
                     QVFLDLYPVSGGFIDWGYEHFGAYTFTNELWESPLDYDKNGEVTEKEMLKWNDEIMHG
                     KAFVDWHPFKHPTLGEIEIGGWDKFHTRMPPTDYLEDLCLRNCLFTLYNAEMMAQLKI
                     KEVQTQPAAGAVKLTVTVTNEGFMDTNTGMADSVKISKPVIVRLEHGPEVEVISGGEK
                     VKHTDRVRLATEGERKVVPERIDLGAVRGRSERLVEWTVVKKSSSKAQIKITVTSEKG
                     GVDERVVSL"
     gene            2191..2628
                     /locus_tag="AC16GM_02830"
     CDS             2191..2628
                     /locus_tag="AC16GM_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922642.1"
                     /translation="MSAVEPYGRWVLFLSVVSFFFSSCGKSMSPNEGQVIRAQLGNFE
                     RALDSRQDSTVALFFVPQKGGERTAAQGLLRQLYSQPEIARVRLTNRRIEVVKKEATV
                     LCTVEALDAAGQKVASIPDRRAMLRLVKQHGTWLVTSYELTAL"
     gene            2609..3352
                     /locus_tag="AC16GM_02835"
     CDS             2609..3352
                     /locus_tag="AC16GM_02835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HIR2922643.1"
                     /translation="MNLPPSDQSKRLFAKTRSEKKAYVQKLFADITPEYDRCNRILSL
                     WRDMAWRRKAVASLPSNSVVLDIAAGTGDMAIALLERKNFTGQVVLSDFTLGMLEEAR
                     HKLKERKLERNAFYVVADAENLPFKAGAFDGAMMGFALRNLTDVPACLGETKRVLVNG
                     GRAAFLEISHPENKLFAKFFYFYFYRVLPFLAHPFTRHPYAYKYLPDSLKIFLTQKEV
                     ASLCRQVGFREATYQNLMGGSVAVYRMVK"
     gene            3487..4017
                     /locus_tag="AC16GM_02840"
     CDS             3487..4017
                     /locus_tag="AC16GM_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2922644.1"
                     /translation="MTEKWSKVFKRALILFLATGVWSALFNFVSPRGIPLVGFWPTGK
                     DLEKLEKPLSYQPTDPPLLSMLDASALYQKSDVIFIDARSPADFEKGHIPRAINVPFE
                     EVDLEEFWPKVADRVPKEKQVVTYCGGADCEASLHLARFLKEKGYSNLHIFFGGWKHW
                     TESKMPIEQGPVASSQ"
BASE COUNT          939 a         1073 c         1169 g          850 t
ORIGIN      
        1 tagattccgg tctggttgag gcatatgtta actgggggtt ggttctgaat aaaataggac
       61 aacatgattc agcaattgag aaatatcgaa cagcattgac gcgaaagcca gattttggtg
      121 aggcatataa ggcctatttt aactggggac ttgctctgat tggtttaggc aggcataatg
      181 aggcagttga aaagtttgag aaagcagtcc agaagaaacc cgattatgct gacgctcact
      241 ataatctggg agttgctttg accaaaggaa acagaaagga agaagccata aaagaattgt
      301 caaaagcccg agaactttct attaaacaag gtaagatgga attagttttg aaggcggata
      361 aattgctaaa ccgattgaaa tagtactcgt aaacaaccta gatgactaag gaggaatttg
      421 gatgttgaaa tacacgctgg ttttttttct tgcgctggtg tccttgacct tcgcccaaac
      481 caccagcccg ctcaaagacg ccaaaattca tttggacttc aagcactact acgactacgc
      541 cgagttgacg caggtgctcc acgacctcgc ttcggcctat ccgcaattca cccacttgga
      601 gtcgatgggc aagagtctaa aggggcgcga cctgtgggtg ctgacggtcg gcaatcccaa
      661 gacgggcgac ctcgccaaaa agccggcgat gtacatcgac ggcaacaccc atggcaatga
      721 aatccagggc ggggaggtgg cgctctacac catctactac ctcctcacgc gctacggcaa
      781 ggacgactac atcaccaaac tgttggatac gcggatgttc tacatcgcgc cgtcggtcaa
      841 tcccgatgcg cgggacgcct tcatccatca gccgaacaac ccccacatgc cgcggcaaaa
      901 ctaccgtccc tgggacgaag accacgacgg gctggtcgat gaggacgggc cggaagatat
      961 cgacggtgac gggagaattc tctcgatgcg ggtgaaaaat ccactcggca actggaagaa
     1021 aggggacgac ccgcgcgata tgcgcccccg ccagccggac gagccggggg agtacgattt
     1081 ctacttcacc gaggggatcg acaacgacgg cgacggctta atcaacgaag atgaagcggg
     1141 cggagtcgat ttgaaccgca acttcccggc ggcctggcag ccgcgctacc agcagtacgg
     1201 ggccggggat tacccccttt ccgaaccgga gacgcgctcg accatcgagt ggatcgtcac
     1261 ccacccgaac atcgcggcga tccaatcgta ccacaatgcc gggaatctga ttctgcggcc
     1321 tttcggcttt aaagacgaca agggtttccc gtcccgcgac ctggcgatgt accgggcgat
     1381 gcagaggcgc ggggaggcct ttttacccgg ctacaaggct tggcaggtat tcctcgacct
     1441 ctacccggtc agcggcggct tcatcgactg ggggtacgag cacttcggcg cctatacctt
     1501 caccaatgaa ttgtgggagt cccctctgga ttacgataag aacggtgaag taaccgaaaa
     1561 ggaaatgctc aagtggaacg acgaaattat gcacgggaag gcgttcgtcg attggcaccc
     1621 gttcaagcat ccgactttgg gcgaaattga aatcggcggc tgggataaat tccacacccg
     1681 gatgccgccc accgactacc tggaggatct ctgcctgcgg aactgcctct ttacgctcta
     1741 caatgcggag atgatggcgc agttgaaaat caaagaggtc cagacgcaac cggctgcggg
     1801 cgcggtcaaa ctcaccgtca cagtcaccaa tgagggattc atggacacga acaccgggat
     1861 ggcggattcg gtgaaaattt caaaaccggt gattgttcgg ttagagcacg ggccggaggt
     1921 ggaggtcatt tccggcgggg agaaggtgaa gcacaccgac cgggttcgtc tggccaccga
     1981 aggtgagcgg aaagtggtcc cggagcgtat tgatttgggc gcggtgcggg gccgttccga
     2041 aagactggtg gaatggacgg tggtgaagaa gagttccagc aaagcgcaga tcaagatcac
     2101 cgtaacctcg gaaaagggcg gcgttgacga gagagtcgtc tccctgtagc aggtctgatg
     2161 gttggtcctg gtagcttagc ccttttcagg gtgagcgcag tcgaacccta tgggcgttgg
     2221 gttctgttcc tctccgttgt ttcgttcttc ttttcatcct gcggcaaatc gatgagcccc
     2281 aacgaaggcc aggtcatccg ggcgcagttg gggaatttcg agcgggcgct cgattcccgg
     2341 caggactcca ccgtcgcact cttctttgtg ccgcagaaag ggggcgagcg cactgcagcg
     2401 caggggttgc tccggcaact ctatagccag ccggagattg cccgcgtgcg tttgaccaac
     2461 cgccggattg aggtggtcaa aaaggaagcc accgtcctct gcacggtcga agcgctggat
     2521 gccgctggac agaaagtagc ctccatcccc gaccgacgcg cgatgctccg tctcgtaaaa
     2581 cagcacggta cctggctcgt tacctcctat gaacttaccg ccctctgacc agtcgaagcg
     2641 actcttcgcg aagactcgct ccgagaaaaa ggcctacgtt cagaagttgt tcgccgacat
     2701 cacgccggag tacgaccgct gcaaccggat tctctcgctc tggcgcgata tggcctggcg
     2761 gcggaaggcg gtcgccagtt tgccgtcgaa tagtgtggta ttagacatcg ccgccggcac
     2821 gggtgatatg gccattgcgc tcctggaacg aaaaaatttc accggccagg tcgtgctttc
     2881 cgatttcact ctcgggatgc tcgaggaagc gcgccacaaa ctcaaggagc gcaagttaga
     2941 gcgcaatgcc ttctacgttg tcgccgatgc ggagaatttg ccgttcaaag ctggggcgtt
     3001 cgacggcgcc atgatgggct ttgccctgcg gaatttgacc gacgttccag cctgcctggg
     3061 ggaaaccaaa cgagtgttgg tgaacggtgg tcgggccgct tttttggaaa tctcccatcc
     3121 cgagaacaag ctcttcgcca aattttttta cttctacttc taccgcgtcc tgccgtttct
     3181 ggcgcacccc ttcacccggc acccgtacgc ctacaagtac ctgccggatt cgctcaaaat
     3241 ctttctgacg cagaaagagg tcgccagcct gtgccggcag gtgggcttcc gcgaggccac
     3301 ctaccagaat ctcatgggcg gctcggtggc ggtctaccgg atggtgaagt gattcaagtg
     3361 aagcgacgct gggattgtgc agaatcgagg cattttgcat ctttccagtg gaggttttgg
     3421 agactaacgt aagagacgct gataccgtaa tttttttgca tgattatttt ttaggagcgc
     3481 gagcggatga ctgaaaagtg gagtaaggtt ttcaaacggg cgctgattct gttcctcgct
     3541 accggcgtgt ggtcggcatt gttcaatttc gtctcgccgc gggggattcc cttggtcggt
     3601 ttctggccca ccggcaagga tttagagaag ttagaaaagc cgctctcgta tcagcccacc
     3661 gatccaccgc ttttgagcat gctggatgcc tccgctctct accagaaatc ggatgttatc
     3721 ttcatcgatg cccgttcccc ggcagacttc gagaaagggc atattcccag ggcgatcaat
     3781 gtgccgtttg aggaagttga tttggaggaa ttctggccga aggtggcgga ccgcgttccc
     3841 aaagaaaagc aggtcgtcac ttactgtggc ggcgcggact gcgaggcctc gctccactta
     3901 gcccgcttct tgaaagagaa gggttactcc aacctccaca tcttcttcgg cgggtggaaa
     3961 cactggacgg agagcaaaat gccaatagaa caggggccag tagccagtag ccagtagcca
     4021 gtagccagga a
//
LOCUS       DBNGZD010000107         5256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1684969, whole genome shotgun sequence.
ACCESSION   DBNGZD010000107 DBNGZD010000000
VERSION     DBNGZD010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1684969"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="AC16GM_02845"
     CDS             complement(<1..809)
                     /locus_tag="AC16GM_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR2922645.1"
                     /translation="MTFPATLDRYLLRQFATTLAFAAGTFLALSLIVDIIEHLDSFID
                     QHATGGAILRYYLYSIPAYLVLPVMPVATLMATLFSLGLLARQNEIVAMKAAGVSLYR
                     LVFPLYLFAFGIAVTLMAFGEWVVPAANRLKTDVREREIEKTRYQTVLLANLYVQGTA
                     GRIFHFQTYDPQAKFARQVLVQRFVNGKLAEEVDAQRMLWSGRVWILEDGHQRKFPLA
                     ADSVAPDTFRAFLRLERPDFTEKPEELVERQRNPQLMGYRDLKRYIKLKERS"
     gene            complement(806..2185)
                     /locus_tag="AC16GM_02850"
     CDS             complement(806..2185)
                     /locus_tag="AC16GM_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR2922646.1"
                     /translation="MFVATRYILREQIGPFVFAFAVITFVLVMDFILEVINLVVAKGL
                     SVWTVLELFALNLAWMLALSVPMAVLVATLMAFGRLGADHEVTAFKACGVNPVRLLLP
                     VLAAATVLAGLMVFFNNQVLPEANHRARLLLAAISQKRPTLRIEPNVFIDDVPGYHFI
                     VKKVDPRSGKISGVTIYDQKERPYPRTIIAKRGEIAFAADGTTLVFTLYDGEVHEADE
                     NDPTKYRRVAFDKQTVYMREAGSQLIRQESDYRTDREMSAAEMQAKNRQTRANLDGLQ
                     KQLRTAAAQRVSQLLTGNPLSGGTPSAPPDSAVTARAAVEEQAFALRLQEPASLVTSF
                     QQEINSLAVEIHKKYAIPVACVVFALIGAPLGILVRRGGFGTSAGISLGFFIFYWACL
                     IGGEELADRRFISAFWAMWTANLVLGLAGLWLLFLTQRDRGFPARELLAKPGRFLGRA
                     LRRPRPALP"
     gene            2327..3292
                     /locus_tag="AC16GM_02855"
     CDS             2327..3292
                     /locus_tag="AC16GM_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIR2922647.1"
                     /translation="MLSDLKVGDTFTGFAVLRKKELREYDGGKFLRLELGDRSGRIDG
                     VVWENAALLFPNLFVGDVVKIKGYVSLYRDTPQIKVDTIALAHQGEYERKALVPASEK
                     TTEQLIAEFKTVVTTVTQPQLRKLLDLFATDEPLLRAYAAAPAAKLWHHAYEGGLLEH
                     TLQVVQICDFAARLYAVVERDLLIAGALLHDIGKVRSYQLSTLIDFTDEGRLVGHINT
                     GDQIVCEKIKQIPDFPENLALRLRHLIIAHQGELQLGSPVVPQTAEAFVLHYADELDA
                     KLGALTRIITRERGQGKTWSDYVRLIDRYIYLGESASGGTQTGSE"
     gene            3304..4332
                     /locus_tag="AC16GM_02860"
     CDS             3304..4332
                     /locus_tag="AC16GM_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR2922648.1"
                     /translation="MSFFDLISNDIGIDLGTANTLVFVRGEGIVLNEPSVVAVEVATK
                     KVLAIGAAAKEMLGRTPGEIVAIRPLKDGVIADFEITERLLADFIKRVVRHRYLMKPR
                     IVISVPSGITEVEKRAVRDSAENAGAREVYLIQEPMSAAIGVGLPVDKPAGNMVIDIG
                     GGTSEIAVIALNGIVNNTSIRIAGDEMNEAIVTYLKKNYNLLIGELTAEEVKIKIGSS
                     FPLDKEETMEIRGRDLVAGVPKTMKISSVQVREALAETVDAIVEAVRQALERTPPELA
                     ADILDRGIILTGGGALLRGLDKRLRQETNLPVNVADDPLTCVVRGTGKVLENMNHYSK
                     VLIKSRRD"
     gene            4458..>5256
                     /locus_tag="AC16GM_02865"
     CDS             4458..>5256
                     /locus_tag="AC16GM_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR2922649.1"
                     /translation="MTDQRQPPRTIPELFAASVRAYGQLPALLAFRQRRWQSFSYRQI
                     KEQADQVASGLLTLGLKRGDRVGILSENRPEWGIAYLAIAAIGCVVVPLDMLLKSSEH
                     EEILRDAEIRAVFASGQFLPQLKALRPLVPTLRFLISLEETADRAVVSWSQMTQRTKG
                     QLAFIGPKPDDLLALIYTSGTMSTAKGVMLSQKNITANVAGCAEVVIFGPQDRFLSVL
                     PLSHTYECTAGFLVPLAKGAAVAYARSLKSKEILEDIGATGATLMLGV"
BASE COUNT         1096 a         1534 c         1650 g          976 t
ORIGIN      
        1 gagcgttcct tgagtttgat gtagcgcttc agatcccggt accccatgag ctgcgggttg
       61 cgctggcgtt ccaccagttc ctccggtttc tcggtaaaat cgggacgttc cagccgcagg
      121 aaggcccgga aggtatcggg agccaccgag tcggcggcga ggggaaattt ccgctggtgg
      181 ccgtcttcca aaatccagac gcgcccactc cagagcatcc tctgggcgtc cacttcttcg
      241 gccagtttgc cgttgacgaa acgctgcacc agcacctggc gggcaaattt cgcctgcgga
      301 tcgtaggtct ggaaatggaa aatccgcccc gccgttccct gcacgtagag gttggccaag
      361 agcaccgtct ggtagcgcgt tttttcgatt tcccgttcgc gcacgtcggt tttcaagcga
      421 ttggccgccg gcaccaccca ctccccgaaa gccatcaggg tgaccgcgat accgaaagcg
      481 aaaaggtaga ggggaaaaac cagacggtag agggaaactc cggccgcttt catggccacg
      541 atctcgttct gacgggctaa aagtcccaag gaaaagagcg tggccatgag cgtggccacc
      601 ggcatcacgg gaagcaccag gtaggcgggg atggagtaaa ggtagtagcg gaggatggct
      661 ccgcccgtgg catgctggtc gatgaagctg tcgaggtgct cgatgatatc gacgatgagc
      721 gaaagcgcca ggaacgttcc ggcggcgaag gccagggtcg tggcgaactg ccgcaggagg
      781 taacggtcga gggtggcggg gaaagtcatg gcagcgcggg ccggggacgt cgtaacgcgc
      841 gccccagaaa cctccccggt ttcgccagca gttcccgcgc cgggaaccca cgatcccttt
      901 gggtcaggaa caaaagccag agcccggcta agccgaggac taagttggcg gtccacatgg
      961 cccagaaggc agagatgaag cggcggtcgg ctagctcttc ccccccgatc aagcaggccc
     1021 agtaaaaaat gaagaaaccg aggctgatcc cggccgacgt gccgaacccc ccccgtcgca
     1081 ccagaatacc gaggggggcg ccgatgagcg caaaaacgac gcaggcgacg gggatggcgt
     1141 actttttgtg gatttccacc gccagggaat tgatctcctg ctggaaactg gtcactaatg
     1201 aggccggctc ctggagccgg agcgcgaacg cctgctcctc gactgcggct cgggcggtta
     1261 ctgcggaatc aggcggggcg ctgggggtcc cacccgagag gggattcccc gtcaagagtt
     1321 gcgacacgcg ttgcgccgcc gcggtccgca gttgtttctg gagcccatcc aggttcgctc
     1381 ttgtctggcg gttcttggcc tgcatttcgg cggccgacat ttcccggtcg gtgcggtagt
     1441 cagattcctg ccggatcaat tgcgacccgg cctcccgcat gtaaactgtc tgcttgtcaa
     1501 aagccacgcg ccggtacttg gtaggatcat tttcgtccgc ttcgtgaacc tcgccgtcgt
     1561 agagcgtaaa aaccaacgtc gtcccgtcgg cggcaaaggc gatctccccc cgcttggcga
     1621 tgatcgtgcg cgggtagggg cgctccttct ggtcgtagat ggtgacgccg gaaatctttc
     1681 cggagcgcgg atcgaccttc ttgacgatga agtggtagcc ggggacgtca tcgatgaaga
     1741 cgttgggctc gatccgtaag gtcgggcgct tctggctgat ggccgccaga aggagccgcg
     1801 cccggtgatt agcttccggc agcacctgat tattgaagaa aaccatcaag cccgccagga
     1861 ccgttgcggc ggccagaacc ggcaaaagca gccgcacggg gttcaccccg caggctttga
     1921 aggcggtcac ctcgtggtcc gcccccagac ggccaaaggc catcagggtt gctaccagca
     1981 ccgccatcgg caccgaaagg gcaagcatcc aggctaagtt cagggcgaac agttcgagga
     2041 ccgtccagac cgaaaggccc ttggccacga cgagattgat cacttcgaga atgaagtcca
     2101 tcaccagaac gaaagtgatc acggcaaagg cgaagacaaa agggccgatc tgctcgcgca
     2161 ggatgtagcg ggtggcgacg aacatcgcgc ggcaatatac gggagagcgg tgccgcaaca
     2221 atcgcttttc cttgacaacc gaatggcagc ggctttatat gcagacattc ccccccgcgt
     2281 gtggcggggg atttccgaaa gggagaagcg ataagtcagg gatgcaatgc tgagcgacct
     2341 caaggtaggg gatactttca ccggctttgc ggttctccgg aaaaaggaac tgcgggagta
     2401 cgacggcgga aaattcctgc ggctggagct gggcgaccgc tccggccgga tcgacggcgt
     2461 ggtctgggaa aatgccgccc tgctctttcc caatctcttc gtgggtgacg tggtgaaaat
     2521 caaaggttac gtctccctgt accgcgacac cccgcaaatc aaagtcgaca ccattgcgct
     2581 ggcgcaccag ggggagtacg agcggaaggc gctcgtcccg gcctcggaga aaaccactga
     2641 gcagttaatc gccgagttca aaaccgtggt gacgacggtc acccagcccc agctccgaaa
     2701 actgctcgat ctctttgcca ccgatgagcc cctgctgcgg gcgtatgccg ccgcgcccgc
     2761 ggccaaactc tggcaccacg cctacgaggg gggactgttg gagcacaccc tgcaggtggt
     2821 gcagatctgc gatttcgccg cccggctgta cgcggtcgtc gagcgcgacc ttttgattgc
     2881 cggcgcactc ctgcacgaca tcggcaaggt gaggtcctac cagctttcga cgttgatcga
     2941 ctttaccgac gagggacgct tggtggggca catcaacacc ggcgaccaga tcgtttgcga
     3001 aaagatcaaa caaattccgg attttccgga aaacctggcc ctgcggttga ggcacttgat
     3061 cattgcccac cagggagaac tgcaattggg gtcgcccgtg gtgccgcaga cggccgaggc
     3121 cttcgtgctc cactacgccg acgaattaga tgccaagctg ggggccctca cccgcatcat
     3181 cacccgggag cgggggcagg gcaaaacctg gagcgattat gtgcgcttaa tcgaccgata
     3241 tatatactta ggtgaatccg cctcaggcgg aacgcaaacc ggaagcgagt gaatggaggc
     3301 aggatgtctt tttttgattt gatctccaat gacattggga tcgatctggg gaccgccaac
     3361 accttggtct tcgtgcgggg agaggggatc gtactcaacg agccctcggt ggtggcggtg
     3421 gaagtggcga ccaaaaaggt tctggcgatc ggcgcggcgg ccaaagagat gctgggccgc
     3481 acgccgggag aaatcgtcgc catccggccc ctcaaggacg gggtcatcgc cgacttcgag
     3541 atcaccgaga gacttttagc cgatttcatc aaacgggtgg tgcggcaccg ctacctgatg
     3601 aagccgcgga tcgtgatctc ggttccttcc ggcatcaccg aagtggagaa gcgggcggtg
     3661 cgcgactcgg cggagaacgc tggcgcccgg gaggtctact tgatacagga gccgatgtcg
     3721 gccgccatcg gcgtcggctt gccggtggac aagccggccg ggaacatggt gatcgatatc
     3781 ggcggcggca cgtctgagat cgccgtgatc gccttgaacg gcatcgtcaa caacacctcg
     3841 atccggattg ccggcgacga aatgaacgaa gcgatcgtca cctacctcaa gaagaattac
     3901 aatcttttga tcggggagct gactgctgaa gaagtcaaaa ttaaaatcgg ctcctcgttc
     3961 cccttggaca aagaggaaac tatggagatt cgcggccgcg acctggtggc tggcgtgccg
     4021 aaaacgatga aaatctcctc ggtccaggtg cgggaagcgc tcgcggaaac cgtcgatgcc
     4081 atcgtcgaag cagtccggca ggcgctggaa cggacaccgc ccgagctggc ggcggacatt
     4141 ctcgatcgcg gcattatcct cacgggcggc ggggcgctcc tgcgcggcct ggataagcgt
     4201 ctccgtcagg aaaccaacct gccggtgaac gtggcggacg atcccttgac ctgcgtggtg
     4261 cgcggcaccg gtaaggtttt ggagaatatg aaccactact cgaaggtcct tattaagagc
     4321 cggcgcgact aaagagaggc ggtatcagga cgatacgacc caccccgcct cccgcggggt
     4381 tttttgtccc ttgtggctat gtccggcgtc cttcatatta ttattcgtgt ccttacctcc
     4441 acacacaaag gaagggtatg acggatcaga ggcaaccgcc gcgtaccatt cctgagctat
     4501 tcgcagccag cgtccgggcc tacggacaac tgccggccct gctcgcattt cgccagaggc
     4561 ggtggcagag tttttcgtac cgccagatca aagagcaggc cgatcaggtg gcgagcggac
     4621 tcttgacctt ggggctcaag cgcggagacc gggtcggcat cttaagtgaa aatcggccgg
     4681 agtgggggat tgcctacctg gccatcgccg ccatcggctg cgtcgtcgtc ccgctcgaca
     4741 tgctccttaa atcctcagag catgaggaga ttctgcgcga cgccgaaatc cgggcggttt
     4801 tcgcctccgg ccagttttta ccccaattga aagctctgcg cccgctggtg ccaaccctgc
     4861 gttttttaat ctcgctggag gagaccgccg accgcgcggt ggtttcctgg agccaaatga
     4921 cccagaggac caaagggcag ctggccttca tcggccccaa accggatgac ctattagcgc
     4981 tgatttatac ctccggcacg atgtcgacgg ccaaaggggt gatgctctcg cagaaaaata
     5041 tcacggccaa tgttgccggc tgcgcagaag tggtcatctt cggcccccaa gatcggttcc
     5101 tttcggtttt gccactgtcc cacacctatg aatgtaccgc cgggtttctg gtgcctctgg
     5161 ccaagggtgc tgcggtggcc tacgcgcgga gtttgaagtc aaaggagatt ctggaggaca
     5221 tcggcgccac cggagccacg ctcatgctgg gagtgc
//
LOCUS       DBNGZD010000108         5082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1824034, whole genome shotgun sequence.
ACCESSION   DBNGZD010000108 DBNGZD010000000
VERSION     DBNGZD010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1824034"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC16GM_02870"
     CDS             complement(<1..209)
                     /locus_tag="AC16GM_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR2922650.1"
                     /translation="MATRSLTQPREVELLRGLALESDLPVYPATGIKVTVIGGGFVGL
                     VAAAGFAQFGHTVALVEKISDKLNEL"
     gene            complement(524..1912)
                     /locus_tag="AC16GM_02875"
     CDS             complement(524..1912)
                     /locus_tag="AC16GM_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2922651.1"
                     /translation="MALVLVIDDEILLGRSLSRALSQRGHDASAVGTAEEGLSLLEKL
                     APDIVLLDMQLPGLSGLDALKKIREADPNILVIIVTAYGTIASAVEAMRVGAVDFLRK
                     PLDTDEVAIAIERALAHARLARTVSFYQSKEAQRLAEEEFIGQSPKAREVQAWLKKVT
                     ELKVAKASDLPPVLVLGETGTGKDLVARLLHYRSPFAGLPFIEVNCPTLPQGLEEAEL
                     FGYEKGAFTDARQCKRGLAEVAEGGTLFLNEIGDLAPETQAKLLRLIEHKKMRHVGGL
                     RDVSIDLRIIAATNRNLAQAVKTGHFRQDLFYRLDHVTITLPPLRERGEDIILLADHF
                     LKKVSGSSGGAKKLSDAAHRVLLVYHWPGNVRELRHLMERALLLSSGTFIEPSDLRLQ
                     TADGAAVTIAPGQKVKVEFGPHGIDIEEVEKELISRALDEAGGNVSEAARMLKLGREA
                     MRYRIQKFGLES"
     gene            complement(1914..3362)
                     /locus_tag="AC16GM_02880"
     CDS             complement(1914..3362)
                     /locus_tag="AC16GM_02880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2922652.1"
                     /translation="MTIRSKLLFSYLSLLLAVVAIGVAAFWAAGRWRQSAAELSRTYN
                     QGLAAERLRADIYRQIAAGTDFLRGDRQAEQEFWDIQERVTAHLQQLKASAKAPEEID
                     HLGAREETQKELAYFAGEIFDAPMQTARNPKSDPGEKLDDIGEEVTSDVAALNQYYQV
                     REKESLAAASRSGNLTAYLVGGAALLAFVELLAMVFLLQRWLAGPIAEVGRVTARISR
                     GDFGARVARESTDEWGELSGAVNRMAESLKSYEHRLRAQERLAALGEVAAFAAHNIRN
                     PLAGIRAASQVMLAECVEERTAVEAREALTGIMQSVDRLDVWIGRLLNFAKPLALQPE
                     KADLRLVVAETLRLLNPALKEKKVKITTRVPEDLPPVFADPALLEQVLSSIITNAAEA
                     TAGDGSVEIQAVAASGEVSVKVSDTGKGMTPAFLERVFKPFVTDKPGGTGLGLAQAKK
                     IVDLHGGDITIQSRPGAGTTVTVHLPVWREGT"
     gene            complement(3359..4306)
                     /locus_tag="AC16GM_02885"
     CDS             complement(3359..4306)
                     /locus_tag="AC16GM_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR2922653.1"
                     /translation="MKAFVTGGAGFIGSHLVSFLLKKGYAVTILDDFSSGRRENLCGL
                     EAATLVEGDVRDFEVVCQTAKGADYVFHLAAVVSVLGSLEDPQTTWEVNLKGSYNVLE
                     AARRAGVRRVIFISSSAVYGEYPKLPFKETFPAQPESPYALSKLMGEELCSLYWRLYQ
                     MEAVALRLFNVYGPRQNPFSQYANAIPNFTRTLLTGQTPTIYGDGEQTRDFVAVSDVV
                     RAALLAGRVKKTVGQVVNVGSGKRLSVNRLFSEIQKILQTDARPQFFPRKQGDVLHTW
                     ADLRRARTVLGFVPRADFARALRETVLWFKNSGIFSRAR"
     gene            complement(4510..>5082)
                     /locus_tag="AC16GM_02890"
     CDS             complement(4510..>5082)
                     /locus_tag="AC16GM_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIR2922654.1"
                     /translation="PMEDGYVTISRALVTLAFPAKFMLAGAMNPCNCGFFGDPGHECS
                     CTPTQIQKYMSRISGPLLDRIDIHIEVPAVKFRELAGQPTGESSAAIRERVNRARQVQ
                     LERFKSEKSIYCNAHMQSREIRKYCLIDDKSQELLRLAMQKLGLSARAFDRILKVSRT
                     IADLEGNAEIQPQHVSEAVQYRSLDRNLWL"
BASE COUNT         1053 a         1496 c         1449 g         1084 t
ORIGIN      
        1 agttcattca gcttatcgga gatcttttca actaaagcga cggtatgacc gaactgggca
       61 aagccggcgg ccgccaccag gccgacgaac ccgccgccaa tgacggtcac tttaataccg
      121 gtggcgggat agaccggcag gtcggactcc agagccagcc cccggaggag ctcgacctcc
      181 ctcggttgcg ttagcgaacg ggtcgccatt acaatttctc tctcccggaa tacgctgcgt
      241 cagctttttc agacaatgtc atctttaata aaattcttgt ttcggagaat tgtcggtcac
      301 tatctcttaa aggtcgaatc ctcagactgg gcaaagaatt ctcctccagg ctgtcaatgg
      361 atttgaaaaa tttgtttcgc tgtaagatca agtagtatca aaaactatgc cgaaaaagta
      421 tttctgaata agttattgat gagtaatagg ttaaatcata caaataaatc gagattacag
      481 agaaaactgg tttaattaaa ccaaactggt ttatttcaac caatcacgac tccaagccga
      541 atttctgaat ccggtaccgc atcgcctccc ggcctaactt caacatgcgt gccgcttcgg
      601 aaacatttcc cccggcttca tccagggccc ggctaatcaa ctccttctcc acttcttcaa
      661 tgtcaatccc gtgcggcccg aactcgactt tgactttctg tcccggagcg atggtcaccg
      721 ccgcaccgtc agcggtttgc aggcggaggt cggacggttc gatgaacgtt ccagaggaaa
      781 gtagcaacgc ccgctccatc aggtggcgca gttcccggac gttgccgggc cagtggtaga
      841 ccagcaagac acggtgggcg gcgtcgctca atttttttgc gccgccggag ctcccggaga
      901 cttttttcaa gaaatggtcg gccaacaaaa tgatgtcctc tccccgctca cgcagaggcg
      961 gcagcgtgat ggtcacatgg tcgagccggt agaagagatc ctggcggaag tggccggttt
     1021 tgactgcttg ggccagattc cggttcgtgg cggcgataat gcggagatcg atggagacat
     1081 cccgcaggcc gcctacatgc cgcatttttt tgtgttcgat caagcgcagc aatttggctt
     1141 gcgtttcggg cgccaaatca ccgatttcat tcaaaaataa cgttccgcct tcggccactt
     1201 cggccaggcc acgtttgcac tggcgggcgt cggtgaaggc ccccttttcg tagccgaaca
     1261 gctccgcttc ctcaagtccc tgcggcaacg tgggacagtt gacctcgatg aacggcaggc
     1321 cggcaaaggg gctccggtag tgcagaagcc gggcgaccag atctttgccg gtgccggttt
     1381 cccccagaac cagaaccggc ggcaggtcgg aggctttggc cactttcaac tcggtcacct
     1441 ttttgagcca ggcttgcacc tcacgggcct tggggctctg gccgatgaac tcttcctccg
     1501 ctaagcgctg agcttccttg ctttggtaga aggaaaccgt ccgggccagc cgggcgtggg
     1561 ccagggcccg ctcgatagcg atggccacct cgtcggtgtc caggggcttg cgcaggaaat
     1621 cgaccgctcc caccctcatc gcctccaccg ccgaggcgat ggtgccgtag gcggtgacga
     1681 taatgaccag aatgttcgga tcagcctcgc ggattttttt cagtgcatcc agacccgaaa
     1741 gaccggggag ctgcatatcg agcaggacga tgtcgggggc gagcttctct aagagcgaca
     1801 ggccttcctc ggcggtgccg accgcggagg cgtcgtgtcc ccgctgggaa agagcccggg
     1861 agagcgaacg ccccaggagg atttcgtcgt cgatgacgag caccagggcc atgttacgtt
     1921 ccctcgcgcc aaaccggtag gtggaccgtc accgtcgtcc cggcgccggg ccggctttgg
     1981 atggtaatgt cgccgccgtg caggtcaacg atcttcttgg cctgcgccag ccccagcccg
     2041 gtgccgcccg gtttatcggt gacaaaaggt ttgaagaccc gctccaggaa ggcgggtgtc
     2101 atccctttac cggtgtcgga aactttcaca gagacttctc cagacgcagc caccgcctgg
     2161 atctccacgc tgccgtcgcc cgccgtcgct tcggcggcat tggtaataat ggaagataaa
     2221 acctgctcaa gcaaggccgg atcggcaaag accgggggaa gatcctctgg cacccgggtc
     2281 gtgatcttga cctttttttc tttcagcgcg gggttaagca ggcgtaaagt ttccgctaca
     2341 accaggcgga ggtcggcttt ctccggctgg agtgccaagg gtttggcgaa attcaaaagc
     2401 cggccgatcc agacgtccaa cctgtccacc gactgcatga tgcccgtgag cgcctcccgg
     2461 gcctcgactg cggtgcgttc ctctacgcac tctgccagca tcacctggga agcggcgcgg
     2521 atgccggcca atgggttgcg gatgttgtgg gcggcgaagg cggcgacttc ccccagggcg
     2581 gccagccgct cctgcgcccg gaggcggtgc tcatagcttt tgagcgactc ggccatccgg
     2641 ttgacggcgc cggataattc cccccactcg tcggtgcttt ccctggcgac gcgcgctcca
     2701 aaatcccccc ggctgatgcg ggccgtgacc cgtccgacct cggcgatggg gccggccagc
     2761 cagcgctgca acaggaagac catggccaga agctcgacaa acgccaaaag cgcggctccc
     2821 ccgacgaggt acgcggtcag atttccggaa cgggaagcgg cagccagtga ttctttttcc
     2881 cgaacttgat agtactggtt caacgccgcc acatcggagg tgacctcttc gccgatgtcg
     2941 tctaactttt cgccggggtc acttttcggg ttgcgcgccg tttgcatcgg cgcgtcgaaa
     3001 atttcaccgg cgaagtacgc cagttctttc tgcgtctctt cccgggctcc gaggtggtcg
     3061 atttcttccg gggctttggc ggacgccttc aattgttgca ggtgcgcggt gacccgctct
     3121 tgaatgtccc agaactcctg ttccgcctgg cggtcgcctc tgaggaaatc ggtaccagcg
     3181 gcaatttgac ggtagatgtc ggcccggagc cgctccgccg ccaatccctg attgtaggtg
     3241 cgggaaagtt cggccgccga ctgccgccag cgcccggcgg cccaaaaagc ggccacgccg
     3301 atggcaacca ccgccaaaag cagggaaaga taactgaaaa gtaacttgga gcggatggtc
     3361 atcgggctcg cgaaaaaatg ccggagtttt tgaaccagag cacggtttcc cgcagcgccc
     3421 tggcaaagtc ggcccgggga acaaaaccta agacggttcg ggcgcggcgc agatcggccc
     3481 aggtgtgcag cacatccccc tgttttcggg ggaagaattg cggcctggcg tcggtttgca
     3541 aaatcttttg gatttcggaa aaaaggcggt tgacggaaag acgtttgccg gagccgacgt
     3601 taaccacctg tccaaccgtt ttcttgaccc gaccggccaa aagcgccgcc cgcaccacat
     3661 cggagacggc aacaaaatca cgggtctgct cgccgtcgcc gtagatcgtg ggggtctgcc
     3721 cggtcaaaag cgtgcgggtg aagttcggaa ttgcgttggc gtattgggaa aagggattct
     3781 ggcgcggacc atacacgttg aacagccgca gggccaccgc ttccatttgg tacagccgcc
     3841 agtacagcga gcagagttct tctcccataa gtttggacag ggcgtagggt gattccggct
     3901 gtgccggaaa ggtttctttg aagggcaatt tggggtactc gccgtacacg gccgatgaag
     3961 atatgaaaat cacgcgccgg accccggcgc gtcgggccgc ttccaggaca ttgtagctgc
     4021 ccttgagatt cacctcccag gtcgtctggg gatcttcgag agagcccaga actgatacca
     4081 ccgccgccag atgaaaaaca tagtcagctc ctttggctgt ttggcagacg acctcaaagt
     4141 cgcgcacgtc cccttctacc agtgtagccg cttcaagtcc gcagaggttc tcccggcggc
     4201 cgctggaaaa gtcatctaga attgtaacgg cgtacccctt tttcagcaaa aacgatacaa
     4261 ggtgggaacc gataaaaccc gccccgccag tgacgaacgc tttcacgtca gtcctttttg
     4321 gtgtctcttt ggacgccaca taatcattca aggtaatggg tgcgtttctt attcacaagg
     4381 ttttgctcat tccttgtgga tttgcgagta gagttgaaca aaacgaggcg gactctttac
     4441 tctgggaaca ggtaggtttt cataactaaa cacttatggc aaccggatcc tttttcaccg
     4501 cctatgttct cacagccata gattccggtc cagcgagcgg tactgcaccg cctccgacac
     4561 gtgctggggt tgaatctcgg cattgccctc caagtcggcg atcgtccgcg acactttcag
     4621 gatgcggtca aacgcccggg cggagagacc caacttttgc atcgccagcc gcaggagttc
     4681 ctgcgatttg tcgtcaatga ggcagtactt ccggatttct ctagactgca tgtgtgcgtt
     4741 gcagtaaatc gacttttcgc tcttgaagcg ctccagttgt acctgccgcg cccggttcac
     4801 tcgctcacgg atagcagccg aactctcacc cgtcggctga cccgctaact cgcgaaattt
     4861 tactgccggc acttcaatgt gaatgtcaat ccgatccaaa agcggcccgg aaattctcga
     4921 catgtacttc tggatttgcg tcggcgtgca ggaacactcg tgtccgggat cgccaaaaaa
     4981 gccacagtta caggggttca ttgcgcccgc taacatgaat ttggccggaa acgcgagcgt
     5041 caccaaagcg cgggaaatag tgacgtagcc atcctccatc gg
//
LOCUS       DBNGZD010000109         3822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1824329, whole genome shotgun sequence.
ACCESSION   DBNGZD010000109 DBNGZD010000000
VERSION     DBNGZD010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1824329"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC16GM_02895"
     CDS             <1..503
                     /locus_tag="AC16GM_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2922655.1"
                     /translation="DAILDTAKVRRFMGTVNDRLYLMDVLGYDLANAQAVARYRSASG
                     DTAFDGKPCGVYFPGSVTQQFKTIYLAFPLYYLTETSARSIFLWAMQQFGEPGATCAL
                     AGDVDGDGFRTITDVVALVNYVVFGDPLPTGEVCADVTVEGTIDLIDIICLLNHVVFQ
                     TPVPCG"
     gene            617..2437
                     /locus_tag="AC16GM_02900"
     CDS             617..2437
                     /locus_tag="AC16GM_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2922656.1"
                     /translation="MVRWFLRVILWVLLFPWPQALGQVVADTHYVSLTGSNLFPYTSW
                     ATAALKIQDAVNASDSGDAVLVDTGRYQLDTTLTLKPHILLRGKGMDSTIILSVRDLN
                     WAIAINPGDTVEHLWVKAAIGSGAGFHDIYSDLLPSFFVRNCKVTDFDDGMNFGFSRG
                     IISNNLLVGNDVEIDVCCDGIYLIENNTLIHAHAFNAINAAPGLQKGFIQKNFIILKE
                     DPFGNKSTGINIFFDADTAIIRNNIITSPSPNQPGVLEGMLVDLRVLEVQNNTISNLS
                     HDGLARSIGIDMSPQSAVPFRVVNNIIDSANVGVSILQGHGTVAYNDFWRVLTQDIEL
                     RNGAILDTNSGNITRDPMFVDSLDFHLQKHSPCIDAGHPGILDPDGSRSDIGAFGGLL
                     GETYIYQDLPPKAPQNLRVTFADTVLVLQWQKNAESDLAGYLLFKDSVPGFPVDSSHL
                     LSSLSPQDSTALDFSFRLGGSYYYRLVAFDNALQASTPSNEASIIGTPVAETHDPNLP
                     RVYSLSQNYPNPFNPSTVLEYTLPDVGAQPAPVRLVIYNLLGQAVRTLVDQKQHPGRY
                     RTVWDGRDDQGKDLPSGIYFAKLLIYDVALTKPTKMVLVR"
     gene            2452..>3822
                     /locus_tag="AC16GM_02905"
     CDS             2452..>3822
                     /locus_tag="AC16GM_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922657.1"
                     /translation="MRRILQLWLSLSVVLLNTVTAGANPQKFKFGHWPYGLLAEPGNG
                     YLISQWTDTLHFNFIIGGNEPATVLNDCLAKNLKVVEDNVVNPLNETYWPFYYSASHY
                     TAWQAEGNPGYNERLIFTGGDEVQDGAIRAHRFRPGVDPAGSVQVGPAYTQEVVFRGV
                     PRDSLGEFPKVEYTAEFHLRTDLIDTAHQPVAILQVQIGIFVCTDSTGGRCNSARYAD
                     SVVAIDTVFGNEIDTSYLPFTLIYRPRQYTIDKDAVYNVQFKVNWFGTKSLYLDKIVV
                     YDQVGKDLIVNHFGDADILNYVDTHNDAHPAVAGWYLAEEIQYPDLLQPWKDVDSLLR
                     VSGKKRGYAVNNATDFQAVVQPKEVAIDLYPLFGGFSTATDTAPPASLQNAWNLYLYG
                     DLYAVPHLGLDSLSRIAKQTNREFHVTAQAFSFDTLNRGPGGGWYPWWRKPTNSELRC
                     MTNLALA"
BASE COUNT          838 a         1079 c          994 g          911 t
ORIGIN      
        1 ctgacgccat cctggacacc gccaaagtgc gccggtttat gggcacggtg aatgaccggc
       61 tctacctcat ggatgtcctg gggtatgatt tggccaacgc acaagccgtc gctcgttatc
      121 gctccgcctc gggcgacacg gcttttgacg gcaaaccgtg cggcgtctac tttccgggtt
      181 cagtgaccca gcagttcaaa accatctacc tggcattccc cctctactat ctcaccgaaa
      241 ccagcgcccg gagcattttc ctgtgggcca tgcagcagtt tggcgaaccc ggcgcaacgt
      301 gcgccctggc cggtgacgta gatggcgacg gattccgaac catcaccgat gtggtcgcgc
      361 tggtcaacta cgtggtcttc ggggatcccc tgcccaccgg cgaagtctgc gccgatgtca
      421 ccgtcgaggg gaccatcgat ttgattgaca ttatctgcct gctcaaccac gtcgtcttcc
      481 agacgccggt accctgcggg taaggaaagt ttcggataaa agagccacgc tagctcctga
      541 tgggctcata aaattgtttg cctgattttc ctcagcgggt gttatattcc cttcagcctc
      601 gcttgaggag gtatcgttgg ttcgctggtt cttgagagtt atcctttggg tgcttctgtt
      661 cccctggccg caggccttag gccaggttgt tgccgacacc cactacgtct ccctgaccgg
      721 ctccaacttg ttcccctaca cctcctgggc caccgctgcc ctgaaaatcc aggacgccgt
      781 caatgcctca gattccggcg atgcggtatt ggtggatacc ggccgctacc agttagatac
      841 caccctgacc cttaagccgc acatcctctt gcggggcaag gggatggatt cgacgatcat
      901 tctttcggta cgcgatctga attgggctat tgccatcaat cccggcgata ccgttgagca
      961 tctctgggtt aaagcagcca taggttcagg tgctggattc cacgatatct actcagatct
     1021 tctgccgagt ttctttgtta gaaattgcaa agttacagat tttgatgatg gaatgaattt
     1081 cggattttca cgcggaataa tttctaacaa tctcttagtg ggcaacgatg tcgaaatcga
     1141 cgtttgttgc gacgggattt acctaatcga aaacaatacg cttatccacg cgcacgcatt
     1201 caatgccatt aacgcagccc ccgggttaca gaagggcttc attcaaaaga acttcattat
     1261 attgaaggaa gatcccttcg ggaacaagtc aaccggcatt aatattttct tcgatgccga
     1321 cacggccatc atccgcaata acatcatcac cagcccttct cccaatcagc ccggcgtcct
     1381 ggaaggcatg ttggtggacc tccgggtgct ggaagtgcag aacaacacca tctccaatct
     1441 aagtcacgat ggtctggcgc gatccattgg cattgacatg tcaccccaat ctgcagtgcc
     1501 ttttcgggtg gtgaacaaca tcattgactc ggccaacgtt ggggtgtcca ttctgcaagg
     1561 ccatggcacc gtcgcctaca acgatttctg gcgcgtcctc acacaagaca tcgagctgcg
     1621 caacggagca attctcgaca cgaattcggg caacatcacc cgcgatccaa tgtttgttga
     1681 ttccctggac ttccacctcc agaaacactc tccctgtatt gatgccggcc atccggggat
     1741 tttagacccg gacgggagcc gttcggacat tggcgctttc gggggactgc tgggggagac
     1801 ctacatttac caggacctgc cacccaaagc gcctcagaat ctgcgggtga cgtttgcgga
     1861 tacggtcctg gtgctccagt ggcagaagaa cgccgaatca gacttagcgg ggtatctcct
     1921 gttcaaggac tcggtccccg gctttccggt agattcttca catcttctga gcagcctttc
     1981 tcctcaggat tcaacggctc tggacttcag ctttcgactg ggtggttctt attactaccg
     2041 gctggtggct tttgacaatg cgctgcaggc gagcacccct tccaatgaag caagtattat
     2101 tggcacaccc gttgccgaga cccacgatcc taatcttccg cgagtttaca gcctatccca
     2161 gaactatccg aacccgttca atccgtctac tgttttggag tacaccttgc ccgacgtggg
     2221 cgcgcagcca gcgccggtac ggttggtcat ttacaatctc ttaggccagg ccgtgcgcac
     2281 tctggtcgat cagaaacaac acccgggccg ctaccgtacc gtctgggatg gcagagatga
     2341 ccagggcaaa gacctgccca gtggaattta ttttgccaaa ttgttaatct atgacgtggc
     2401 actaaccaag ccgaccaaaa tggttctggt tcgctgacag aggaggttcc gatgcgtcgc
     2461 attctgcaat tgtggttgag tctaagcgtt gttctcctga acacggtcac ggcgggagcc
     2521 aatccccaga agttcaaatt cgggcactgg ccgtatggac tcctggctga acctggcaac
     2581 ggctatttga tctctcaatg gactgacacg ctccatttca acttcatcat cggcggaaat
     2641 gaaccggcaa ctgtcttgaa cgactgcctg gccaagaacc tgaaggtcgt ggaggacaac
     2701 gtcgtcaatc ctttgaatga aacctactgg ccattctact attctgcaag ccattatacg
     2761 gcctggcagg ccgaaggtaa cccggggtac aacgagagac tgatttttac tggtggcgac
     2821 gaagtgcagg atggcgcaat cagggcgcac aggtttcgcc ccggagttga ccccgcgggt
     2881 tcggtacagg tgggcccggc ctatacgcag gaggttgtct ttcggggggt gccgcgagat
     2941 agcctgggag aatttccaaa ggtcgagtac acggcagagt ttcacctccg cactgacctg
     3001 atcgatacgg cccaccagcc agtggcaatc ctccaagtgc agataggcat ctttgtttgc
     3061 accgatagta ccggtggtcg ttgtaactct gcccgctatg ctgacagtgt cgtagccatt
     3121 gacacggtct tcggcaatga aatcgataca tcgtaccttc cgttcacttt aatctaccga
     3181 ccaaggcagt acaccatcga caaagatgcg gtctacaacg tccagttcaa agtcaactgg
     3241 ttcgggacca agtctctcta cctggacaaa atagtagttt acgaccaggt ggggaaagat
     3301 ttaattgtta atcactttgg agatgcagac atcttgaact atgtggatac tcacaacgat
     3361 gctcaccctg cggtggctgg ctggtacctg gccgaggaaa ttcaataccc ggacctctta
     3421 cagccctgga aggacgtgga tagtctcttg agggtgagcg gcaagaagcg gggctatgcg
     3481 gtgaataacg cgaccgattt ccaggcggta gtccagccga aagaggtcgc aattgacctg
     3541 tatccactct tcgggggctt ctcgaccgca accgataccg cgcccccggc atcgctccag
     3601 aacgcctgga atttgtacct gtacggtgac ttgtatgcgg tgcctcacct gggcttggat
     3661 tccctttccc gaattgccaa acagaccaat cgggaatttc acgtcacggc ccaggcgttt
     3721 tctttcgata ccttgaatcg ggggccaggt ggcggatggt atccctggtg gcggaaaccg
     3781 accaactcag agttgcgttg tatgacaaac ctggccttag cc
//
LOCUS       DBNGZD010000110         4019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_921650, whole genome shotgun sequence.
ACCESSION   DBNGZD010000110 DBNGZD010000000
VERSION     DBNGZD010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_921650"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2697
                     /locus_tag="AC16GM_02910"
     CDS             <1..2697
                     /locus_tag="AC16GM_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HIR2922658.1"
                     /translation="TCFPVGMTTVKCVVTDAAGNKDSCTFKITVNDTQKPTITCPADI
                     AKSTDPGKCCAVATFSPTINDNCPGATVVCTPASGFCFPVGMTTVKCVVTDAASNKDS
                     CTFKVTVNNGPPILSFPGGKNRSINLCESICFPVKATDPEGDSVTITKLSAEGGLSCP
                     PQPKPSPDSCEFCFTPTVPGLYRAIFRAADKCGNMTTDTVVITVNNERLFVEGPRDSS
                     LFLCAGDTIKFQVCAHDCDTLTLTKECGPGTFPPERDRSPVCGTLTFVPPSSGLYTWC
                     FKAVDRFGAMAVDTVRFNVTVNSPPNISVTPSLPETVTVGDTVCFTVTGTDIDAGDIV
                     TLTVSPPGFQKQCSPPGPNRTCTFCVTSSEPGTFCLVFKAADNCGATALDTVCITFQQ
                     CFRFVIDTATAFLGQHSVVLCVNNVVEAFDPSVTPNPSGVGGFNFLFAYDPTCLTFQK
                     AEMGQFLKDCQWEFFTFRFGTRGNCGTCPSGFIRVIGIADINDALQHHPPASCFHRNA
                     GQWICLFFEVTRDRTFQNTCCPVRWAWEDCTDNTVSDSTGNFLWVVDSVYNAHGVPID
                     LATEYGIGVDVSNCVSSGGPGKPQTIKCIVFKDGKICIPADSSIDARGDINLNGITNE
                     IADAVLHENCLLYGMSALSSDPLRRQAQIAASDVNNDGLDMTVADLVYQLRIITGDAQ
                     PFPKPAGGASPDVTIDFAERWGELFVASSNRTIDLGGLLLVLKVNGQVKDVVLTGRAG
                     EMGVRFAQTGDQLRILIDTYERGKIIAAGTGEILKITGVRSAEFVEAQASNRGGYPVT
                     IELAKSPVPTQFSLFQNRPNPFNPTTRITLALPVASDYRLVIYNVAGQVVRTFAGQSE
                     AGEVNITWDGTDENGNKVASGIYFYRGAAGTFSATKKMVLIK"
     gene            2817..>4019
                     /locus_tag="AC16GM_02915"
     CDS             2817..>4019
                     /locus_tag="AC16GM_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2922659.1"
                     /translation="MKKLLPQIVFSSLLVWVSVAVAQIGGATPIAADAEFCLRIEKKT
                     YFLGWHGAVVCINNEREASDPDLANGVGVSGFNFLLAYDPVCLTLQKVEPGQFLKDCQ
                     WEFFTYRFGPLGNCGGGCPSEFIRVVALADLNDARQYHPPPACSRRNSGQWVCLFFEL
                     TRDRTFENICCPIRWWFRDCTDNTISDSTGNVLWTVDSVYNAEGVPLDLATEYGSGVD
                     VSNCVSSGGQTKKRVVLKDGEICIPAEPAVDMPGDLNLNGLRFEIGDAVLFENYLLQG
                     LSALSPDPQRRQAQIASADMNRDRQINLADLVYLLCVILGDCQFPPPPQKPAVGVSAG
                     VTIDLIPQGREVAVLSDNRAVDLGGLLLVLKVNDTVADVSLTGKASGMTLRYLQAGDQ
                     LRILVDAHE"
BASE COUNT          813 a         1179 c         1194 g          833 t
ORIGIN      
        1 acctgcttcc cggtggggat gacgacggtg aagtgtgtgg tcaccgacgc tgccgggaat
       61 aaggacagct gcactttcaa gataacggtg aacgacacgc agaagccgac gatcacctgt
      121 ccggcggata ttgcgaagag caccgacccc ggcaaatgct gtgcggtggc gaccttcagc
      181 ccgacgatca atgacaactg tccgggggcg acggtggtct gcacgccggc ttcggggttc
      241 tgcttcccgg tggggatgac gacggtgaag tgtgtggtga ccgatgcggc cagcaacaaa
      301 gacagctgta cgttcaaggt aacggtcaac aacggcccgc cgattttgag cttccctggg
      361 ggcaagaacc ggtccatcaa cctctgtgaa agcatctgct tcccggtgaa ggccaccgac
      421 ccggaaggcg attcggttac catcaccaag ctttcggctg agggagggct ctcctgtccg
      481 ccacagccga aaccctcgcc cgattcctgt gagttctgct ttacgcccac cgtgccgggg
      541 ctctaccggg ctattttcag ggcggccgat aagtgcggca acatgacgac tgacacggtc
      601 gttattacgg tgaataatga gcgactgttc gtggaagggc cacgtgattc atcgttgttc
      661 ctctgtgccg gcgatacgat caagttccaa gtctgcgcgc acgactgcga cacgttgacc
      721 ttaaccaaag agtgcggtcc gggcaccttc ccgccggagc gggatcggtc gccagtctgc
      781 ggtaccttga cattcgtgcc gccgtcatcc ggcctctaca cctggtgctt caaagccgtt
      841 gatcgcttcg gcgcaatggc ggtcgacacg gtacggttca acgtgacggt gaattccccg
      901 ccgaacatct ccgtgacgcc ctccctgcca gagacggtca cagttggcga cacggtctgc
      961 tttacggtca ccgggacgga cattgatgcc ggggacatcg tcactttgac cgtttccccg
     1021 ccgggattcc agaagcagtg cagcccgcca gggccgaatc gaacctgcac cttctgtgtc
     1081 acttcgtcgg agccgggaac cttctgtctc gtattcaagg ccgcggacaa ctgcggcgca
     1141 acggccctgg ataccgtctg catcaccttc caacagtgct tccgtttcgt catcgacacc
     1201 gcaactgctt tcctggggca gcacagcgtt gtgctttgcg tcaacaatgt ggttgaagcg
     1261 tttgacccaa gcgtcacccc caacccgtcg ggtgtgggcg gcttcaactt cctctttgcc
     1321 tacgacccga cctgcctcac cttccaaaaa gccgaaatgg gtcagttcct taaggactgc
     1381 cagtgggagt tcttcacctt ccgcttcggg acgcgcggga actgcggcac ctgcccctcc
     1441 gggttcatcc gggtgattgg cattgccgac atcaacgatg ccctgcagca ccacccgccg
     1501 gcgagttgct tccaccggaa cgcgggccag tggatttgcc tcttctttga ggtcacccgc
     1561 gaccggacgt tccagaacac ctgctgcccg gtgcgctggg cgtgggagga ctgcacggac
     1621 aacacggtct ccgacagcac cggtaacttc ctctgggtgg tcgattcggt ctacaatgcc
     1681 catggcgtgc cgattgatct ggcaaccgag tacggcatcg gtgtggatgt ctccaattgc
     1741 gtctcttccg gcgggccggg caaaccgcag accatcaagt gcatcgtctt caaggacgga
     1801 aagatttgca tcccggccga tagcagcatc gacgcccggg gcgacataaa cctaaacggc
     1861 atcaccaacg aaattgccga cgccgtactt cacgagaact gtctactgta tggtatgagt
     1921 gcgctctcct ccgacccgct taggcgacag gcgcaaattg cggcttccga cgtcaacaac
     1981 gacggcttgg acatgacagt ggccgactta gtttaccagt tgcgaattat cacgggcgat
     2041 gcccagccct tccccaaacc ggcgggcggc gcatctcctg atgtcaccat tgactttgcc
     2101 gagcggtggg gcgagttatt cgttgccagt tccaaccgca ccatcgatct gggcggatta
     2161 ctcctcgttt tgaaggtgaa cggtcaggtc aaggatgtgg tcctaaccgg gagagcgggc
     2221 gagatgggcg tgcgctttgc ccaaacgggc gaccagctgc gcatcctgat cgacacctac
     2281 gaacggggca aaatcattgc cgcaggaaca ggcgagattt taaagatcac gggagttcgt
     2341 tcggctgaat ttgtggaagc tcaagcgtcg aaccggggcg ggtaccccgt aacgatcgaa
     2401 ctggccaaat caccggtgcc aactcaattc tcactcttcc agaaccggcc caacccattc
     2461 aacccgacga cgcgtatcac cctcgcccta ccggtagcca gcgactacag gctcgtgatc
     2521 tacaacgtgg cgggccaggt ggtgagaacc tttgccggac agagcgaggc gggtgaggtg
     2581 aatattacct gggatggtac cgatgagaac ggcaacaagg tggcctcggg tatctacttc
     2641 tacagaggag cggccgggac cttctccgcc accaaaaaga tggtactcat taaataaccc
     2701 cgccgcgggc gggttctgta ggttaacaat aagaaaaatc cccctgccgc aagggggatt
     2761 tttcttgggg aggaagtacg agaaccgggg aagcggatca gaggttggaa aaagcggtga
     2821 aaaagctcct tccacaaata gtattctctt ctttgctcgt gtgggtgagt gtcgcggtgg
     2881 cgcagattgg gggagcgacg ccgattgcgg cggatgcaga gttttgtctc cggatcgaga
     2941 agaagaccta cttcctcggc tggcacggcg cagtggtctg tatcaataac gagcgggagg
     3001 cctccgatcc cgatctggcc aacggggtgg gagtgagcgg tttcaacttt ctcctggcct
     3061 acgacccggt ttgtttgacc ttacagaaag tggagccggg acagttcctg aaggactgcc
     3121 agtgggaatt tttcacctac cggttcgggc cgctggggaa ttgcggcggg ggttgcccct
     3181 ccgaattcat ccgggtggtt gctcttgccg acctcaatga cgcccggcag tatcatccgc
     3241 cgccggcatg ttcgcggcgg aactcaggcc aatgggtgtg tcttttcttt gagttaaccc
     3301 gcgaccggac cttcgagaac atctgttgcc cgatccgctg gtggttccgg gattgcacgg
     3361 acaacacgat ttcagactcg accgggaacg tgctctggac ggtcgactcg gtttacaatg
     3421 ctgaaggcgt cccgcttgat ctggcgaccg agtatggcag cggtgtggat gtgtccaact
     3481 gtgtttcctc gggcgggcaa accaaaaagc gcgtggtact gaaagacgga gagatttgca
     3541 tccccgctga gcctgccgtc gacatgccgg gtgacttaaa cttgaacggc ctgcgctttg
     3601 aaatcggcga cgccgtcctc ttcgagaact acttgctgca agggttaagc gcgctctcac
     3661 ccgatccgca aaggcggcag gcacagattg cttctgcaga catgaaccgc gaccggcaga
     3721 ttaacttggc cgatctcgtt tatctattgt gcgtcattct cggcgattgc caatttcctc
     3781 cgcctcccca gaaaccggcg gtgggcgtct cagccggggt aacgattgac ttgatcccgc
     3841 aggggaggga agtggccgta ctctccgaca accgggcagt tgacttgggc ggactcctcc
     3901 tcgttttgaa ggtcaacgac acagtggccg acgtttctct gaccggaaaa gcgagcggga
     3961 tgacgttgcg ctacctgcag gcgggcgacc agctgcgcat cctcgtcgat gcccacgag
//
LOCUS       DBNGZD010000111         8791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1268847, whole genome shotgun sequence.
ACCESSION   DBNGZD010000111 DBNGZD010000000
VERSION     DBNGZD010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1268847"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /gene="eno"
                     /locus_tag="AC16GM_02920"
     CDS             <1..1208
                     /gene="eno"
                     /locus_tag="AC16GM_02920"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR2922660.1"
                     /translation="RAAVPSGASTGAHEALELRDGDPRRYLGKGVLKAVENVNRTIGP
                     ALIKKGIDLFVQEAMDQFLIDLDGTPEKKKLGANAVLGVSLAWAKAAALAQGVPLYRY
                     LGGDEAHVLPVPLLNVINGGRHADNNLDFQEFMLAPVGAPSFGEALRMGAEIFHTLQK
                     IAKQKGHSIAVGDEGGIAPNLKCEEEALELLSESARQAGYIPGEVLYFALDPAASEFY
                     DSRTGQYVLKGAGKTYTSSQLLDRYERLVDQFPIFSIEDGLAEDDWDGWKELTGRLGE
                     KIQLVGDDLFVTNPSRLSRGIKEKAANAILVKLNQIGTLSETLAVIRQAQKAKWGVVI
                     SHRSGETEDTTIADLAVATNAGQIKTGSLSRSERIAKYNQLLRLEEELGDRARYFGSQ
                     LKVREAVKA"
     gene            1209..1571
                     /locus_tag="AC16GM_02925"
     CDS             1209..1571
                     /locus_tag="AC16GM_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR2922661.1"
                     /translation="MSAQPPIIIRLTATARSRGWQKYLVRFGVLAAFVFLGYSLVGGN
                     FGLLRIRKLTRERQALETQKLHATVELYDLVRLSEQLRTDRRFGEYFARQKYHFARPE
                     EKVYIFRAPESVTGNGDQ"
     gene            1558..2319
                     /gene="recO"
                     /locus_tag="AC16GM_02930"
     CDS             1558..2319
                     /gene="recO"
                     /locus_tag="AC16GM_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR2922662.1"
                     /translation="MAISKGEGIILRERLFGETSKVITLVSPQQGKFALVAKGARGPK
                     SRYGSILDRFAHISFVFYERRGEGMAYLSQADLIRAYFGVAADLSRFACASALVELTD
                     RLVTGEEPVPAIFALLRDALGLCETVGLDDLEKLFWFFCYRMLRELGYRPHLDACAVC
                     QKTVEGENLFFSPEKGGLVCRRCANPRGTYQRIAAGTVTMLKQAEQSSLEEVVRAKIS
                     AQQVGETANLLRGMLTYQVGVTEPIRSLEFMVKAS"
     gene            2322..3647
                     /locus_tag="AC16GM_02935"
     CDS             2322..3647
                     /locus_tag="AC16GM_02935"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR2922663.1"
                     /translation="MAKTKAQPGSDAMEKLVSLAKRRGFIFQSSEIYGGLTSCWDYGP
                     LGVELKNNLKAFWWKAMTQLRDDVEGIDAAILMHPKVWETSGHVASFTDPLRDCKNCK
                     MRFRADEVTNSTCPNCGGELTAARQFNLMFKTHMGPVEDDASVVYLRPETAQGIYVNF
                     LNVIGPARQKVPFGIAQIGKAFRNEISPGNFIFRSREFEQMEMQFFIHPSEDEKWLDY
                     WKEQRLQWYFDLGIRKEKLRFHQHGPKELAHYAKAAYDVQYEFPFGWQELEGIHNRTD
                     FDLSRHKEATGKDLSYFDEKTNTRFVPYIIETSAGVDRTLLVTLVDGYYEEEVKGEKR
                     AVLRLSPKIAPIKAAVFPLVNREGMPDIAMKIFHDLKRYFKVFYDDSGAVGRRYRRMD
                     EAGTPYCITVDSQTLQDETVTIRDRDTMEQERVKSGSLAEVIRKRISFE"
     gene            3760..4344
                     /locus_tag="AC16GM_02940"
     CDS             3760..4344
                     /locus_tag="AC16GM_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922664.1"
                     /translation="MGERTDITDKLVHFTSGKDFEEASGRLRLIIDERRLSGGGEKIR
                     GGYRCVCFTEAPLTSLPHGLVNPRAYSLYSPFGILFEKKWIFDKGGRPVIYQPDVEFD
                     LLPESIRWRHVRYEPDTIDFTWEREWRIKCDYLDFTPEVACIVVPSKEWADWLVAEHD
                     AEQDFLVLQYSQVLDEQLAEQYREEFGWRISVLS"
     gene            4586..4963
                     /locus_tag="AC16GM_02945"
     CDS             4586..4963
                     /locus_tag="AC16GM_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR2922665.1"
                     /translation="MMKYLFKYQTSNIPRARKLRRDMTNAERKPWSLLRRDRCGVYFR
                     RQVPFGPYILDFCSIKIKLVVELDGGQHFVEKGIVADRKRDAALRKQGLAVLRFTDRE
                     VLTNTDGVMHAIYEQIQKRLRAS"
     gene            5111..6559
                     /gene="tilS"
                     /locus_tag="AC16GM_02950"
     CDS             5111..6559
                     /gene="tilS"
                     /locus_tag="AC16GM_02950"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR2922666.1"
                     /translation="MNAVLTKAVKTVQTHHLVKPGEKVVVACSGGPDSVFLVHLFAHL
                     KPLMFLDVAVAHLNHSLRGAESDADEQFTQDLAGQLELPFYARKVDVAALAEKEHLSL
                     EACGRNERYCFFSELAEQLNAEKIATGHTADDQAETVLMHLLRGAGSRGLAGMPLLRG
                     KIIRPLLHLSKKEILAYLESQKIQYRIDRTNEKTDFLRNKIRHHLMPLLEQEYNPSIT
                     AALGRTAEILEQQENYLEWFAQEAVEDCLVSSGEEAVILDSGRFLNYDPVIQRLVVRS
                     VWQKLTGSAFGLGFETVRRVIDDANGQSRLAPLGSRFWVEHTGTQLIFFKKVTQRVEH
                     SVVLPGSYSWGNPPLSLVAEVVEREGIDLFVNGDPAVAYFDWDRLTPPFVLRSVKPGD
                     RFAPLGMGGQKKVGDFFTDLKIPRYQRAKSLVLTSNGEVVWLVGYRISDRFKVTIQTR
                     RVLKAIVRDGLFAPKGKTHPADYIQQGFTKAG"
     gene            6486..7037
                     /gene="hpt"
                     /locus_tag="AC16GM_02955"
     CDS             6486..7037
                     /gene="hpt"
                     /locus_tag="AC16GM_02955"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR2922667.1"
                     /translation="MAYSLQRERPIQPIISNRALQKRVRELGRQLSRDYRTKNPLCVG
                     ILKGSFIFLADLVRHLTIPHEVDFISVASYGEATRAQGSVQLLKDLNRPVGGRHVVIV
                     EDIVDTGLTVEYIRKNLLARGPKSVAILTLLDKKGRRRVPVQLKYVGFAIPNDFVVGY
                     GLDLNEQHRNLPYIGKVAGVPLD"
     gene            7237..>8791
                     /gene="ftsH"
                     /locus_tag="AC16GM_02960"
     CDS             7237..>8791
                     /gene="ftsH"
                     /locus_tag="AC16GM_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR2922668.1"
                     /translation="MVKILNAGRGEVTRISYTEFMGQLDTNNIKTVTFVDKDLQGEFK
                     EKFEKKEGRFLRKYGRFKLHIPFDDPQLLEKLRAKSVEIAAEPRGWDWGAALLSMSPF
                     LVLLVFWLLMLRQMQGGPKGLFSFGKSRARLLTEDKPKVTFSDVAGADEAKEELREII
                     EFLREPAKFQKLGGKIPKGALLLGPPGTGKTLLARAVAGEAAVPFFSMSGSDFVEMFV
                     GVGASRVRDLFDQGKRSAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDG
                     FESNEGVILLAATNRPDVLDPALLRPGRFDRQIVVDSPDVRGREGILKVHTRKIPVGE
                     DVELSVLARGTPGLSGADLANLENEAALLAARRNHPKVTMVDFEDAKDKVMMGTERRS
                     LVISDDEKRTTAYHEAGHALVAKLIPGSDPVHKVTIIPRGLALGLTSYLPFDEKHTHS
                     KQYLETRLVHMMGGRVAERLVFNQLTTGAGNDIEQATVLARKMVCDWGMSEKLGPLTF
                     GNKEEEIFLG"
BASE COUNT         1921 a         2412 c         2683 g         1775 t
ORIGIN      
        1 gccgcgcggc ggtgccgtcc ggcgcctcga ccggcgctca cgaagcgctg gaactgcgcg
       61 acggcgaccc ccggcgctat ctgggaaaag gcgttttgaa agcggtcgag aacgtcaatc
      121 gtaccatcgg tcccgctctg ataaaaaaag gcattgacct tttcgttcaa gaggcgatgg
      181 atcaattctt aatcgacctc gatggcacgc cggagaagaa aaaattagga gcgaatgcgg
      241 ttttaggcgt ctcgctcgcc tgggcgaagg cggcggcttt ggcgcagggc gttcctctct
      301 accgttatct cggcggcgat gaggcgcacg tgctgccggt gccgcttttg aacgtgatta
      361 acggcgggcg gcacgccgac aacaatctcg atttccagga gttcatgctc gctccggtgg
      421 gcgcgccttc gttcggcgaa gccctgcgga tgggcgcgga aatcttccac acgcttcaga
      481 aaattgccaa gcaaaaagga cacagcatcg ccgtcgggga cgagggcggg atcgcgccga
      541 atctcaaatg cgaagaggaa gctttagaat tactctccga aagcgcgcga caggcagggt
      601 acatccccgg agaagttctc tactttgcgc tcgatcctgc cgcatcggaa ttttatgatt
      661 cccgtacggg tcagtacgtc ctgaaaggtg cgggcaagac gtatacttct tctcagttgc
      721 tcgaccggta cgagcggctg gtcgatcaat tccccatttt ctcaattgag gacgggctgg
      781 cggaagacga ctgggacggc tggaaagaat tgaccgggcg tctgggagag aaaatacaat
      841 tggtcggcga tgacctcttt gtcacgaatc cctcccgttt gtcacgcggg ataaaggaga
      901 aagcggcgaa cgcgatctta gtgaagttga atcaaatcgg gacgctgtcg gaaacactgg
      961 cggtcatccg gcaagcgcaa aaagcgaagt ggggcgtggt catttcccac cgctccggcg
     1021 aaacggagga cacgacaatt gccgacttgg cggtggcgac caatgccggg cagatcaaaa
     1081 ccggttcgct ctcccgctcc gagcggatag ccaagtacaa ccagttgctg cggcttgagg
     1141 aagagttggg tgaccgggcg cggtacttcg gctcgcaact gaaagtgcgg gaggcggtga
     1201 aagcgtaaat gagcgcccag ccgccgatta tcatccgcct gaccgcaacg gcccgcagcc
     1261 ggggctggca gaaatatctg gtgcggttcg gcgttctggc ggcgttcgtt tttctgggct
     1321 attcgctcgt gggcgggaac ttcggtctct tgcggattcg caagttgacc cgcgagcggc
     1381 aggcgctgga aacccaaaag ctgcacgcca ccgtcgaact gtacgacctg gtgcgcctgt
     1441 cggaacagtt gcgtaccgac cggcgtttcg gggaatactt cgcccgccag aagtaccact
     1501 tcgcccggcc cgaggaaaag gtctacatct tccgggcccc ggaatcagtc accggaaatg
     1561 gcgatcagta aaggtgaggg gatcatcctg cgcgagcggc tcttcggcga aacctccaaa
     1621 gtaatcacgc tcgtttctcc ccagcagggc aaattcgctc tcgtggccaa aggcgcgcgc
     1681 gggcccaaga gccgctacgg cagcattctc gaccgctttg cccacatcag tttcgtcttc
     1741 tacgaaaggc ggggcgaggg gatggcctat ctttcgcagg ccgacttgat ccgcgcctac
     1801 ttcggcgtcg ctgccgatct ctcccgcttt gcctgcgcct cggcattggt ggaattgacc
     1861 gaccggctgg tgacagggga agagccggtg cccgcgattt ttgccttgct gcgcgacgcc
     1921 ttagggttgt gcgaaactgt tgggctggac gacctcgaga aactcttctg gtttttctgc
     1981 taccggatgc tgcgggaact gggctaccgg ccccacctcg atgcctgcgc cgtctgccag
     2041 aaaacggtgg aaggcgagaa tctctttttc agtccggaaa aaggcgggct cgtctgccgg
     2101 cgctgcgcca acccgcgggg gacctaccag cggattgcgg cggggactgt cacgatgttg
     2161 aagcaggccg agcaaagttc ccttgaagaa gtcgttcgcg cgaaaatctc cgcgcaacag
     2221 gttggagaaa ccgccaatct cctgcgggga atgctgacct atcaggtcgg cgtgacggaa
     2281 ccgatccgct ccttggaatt catggtgaag gcttcctgaa catggccaag actaaagcac
     2341 agcccggctc cgacgcaatg gaaaagttgg tttcgctggc caagcgccgc gggttcatct
     2401 tccagtcctc ggaaatttac ggcggactca ccagctgctg ggactacggg ccgttgggcg
     2461 tcgaactcaa aaacaacctc aaggccttct ggtggaaggc gatgacgcag ttgcgcgacg
     2521 acgtcgaggg aatcgacgcc gccatcctga tgcacccgaa agtctgggaa acctccgggc
     2581 acgtggccag tttcaccgac cccttgcgcg actgcaagaa ctgcaagatg cgcttccgcg
     2641 cggatgaagt cacaaacagc acctgcccca actgcggggg agagctgaca gcggcgcgcc
     2701 agttcaattt gatgttcaag acccacatgg ggccggtgga agatgacgcc tcggtggtct
     2761 atctccgacc cgaaacggcg caggggattt acgtcaactt cttaaacgtc atcgggccgg
     2821 cccggcaaaa agtgcccttc gggatcgccc agatcggcaa ggccttccgc aacgaaattt
     2881 cgccggggaa tttcatcttc cgctcgcggg agttcgagca gatggagatg cagtttttca
     2941 tccacccgtc ggaagacgaa aagtggctgg actactggaa agagcagcgg ctgcagtggt
     3001 attttgattt gggcatccgc aaagaaaaac tgcgcttcca ccagcacggg ccgaaggaat
     3061 tagcccacta cgccaaagcg gcctatgacg ttcagtacga gttccccttc gggtggcagg
     3121 agttggaggg aattcacaac cgcaccgatt tcgatttgtc ccgccacaag gaagccaccg
     3181 gcaaagacct ctcctacttt gacgagaaga ccaacacccg ctttgtgccc tacatcatcg
     3241 aaacctcagc cggggtggac cggacgcttc tggtcacttt ggttgacggc tattacgagg
     3301 aagaggtgaa aggggagaag cgggcggtcc tgcgtctatc tcccaaaatt gcccccatca
     3361 aggccgcggt ctttcccttg gtgaaccggg agggaatgcc tgatatagcc atgaaaatct
     3421 tccatgactt aaagcgttac tttaaggtct tctacgacga ctcgggcgcg gtgggccgcc
     3481 gctaccgccg gatggacgaa gccggcaccc cctactgcat caccgtcgac tcccagaccc
     3541 tgcaggacga aaccgtcacc atccgcgacc gggacacaat ggaacaggaa cgggtgaaga
     3601 gtgggtcgtt agcagaggta attcgaaaga gaattagctt tgagtagagg acagtatttt
     3661 tagtagagtg cgagaccata gagcgtgccc tcaggaagtg cggggcaacc gcaaaaaagc
     3721 cgcggcttaa ccaggttgtt agaagccgta cattgagcta tgggcgaaag aacagacatc
     3781 acagataagc tagtacactt cacgagtggc aaagatttcg aagaggcatc tggtcgcctc
     3841 cgactgatca ttgacgagcg tcggctaagt ggtggtggag aaaagattcg cggcgggtat
     3901 cgctgtgtgt gttttacaga agcgcccctt acttcgttgc cgcacggcct tgttaaccca
     3961 agagcgtatt cactctactc accttttgga attctgttcg aaaagaagtg gatctttgat
     4021 aaaggtgggc gccccgtgat ttatcagccg gatgtggagt ttgaccttct gccagaatct
     4081 atccggtggc gccacgtacg gtacgaaccg gacacaattg actttacgtg ggaacgggaa
     4141 tggagaatta aatgcgacta cctagacttt actccggagg tagcgtgtat agtagttcct
     4201 tctaaggaat gggcagactg gctggtcgca gaacacgatg cagaacaaga ttttctagtg
     4261 ctccagtact cccaagtttt ggatgaacag cttgcagaac agtatcgcga ggagtttggc
     4321 tggcgaattt ctgttttgag ctgaatggca tctaactgcg cggccaaccg ggacatccca
     4381 gaaagtaccg tagccgagga ttccatcctc ggccctgcgg cttttttgag ccctccagtc
     4441 ttttccggga gccaagggtg acccctccct tgccctcccc tcgcagggta gggaaaagta
     4501 cccctcaagg gggagggata agtgttggga gcggagtggt gcgggaaacc cctccctggc
     4561 cctccccacg gaggggaggg aaaagatgat gaagtacctt ttcaaatacc aaacgtcgaa
     4621 tattcccaga gcacgcaaac tccgccggga catgaccaac gcggagagaa aaccgtggtc
     4681 gctcttgcgg cgggatcgct gcggagttta ttttcgacga caggttccct tcggtccgta
     4741 catcctggat ttctgctcca tcaagataaa actggtcgtg gagttagatg gcggccagca
     4801 cttcgtagaa aaagggattg ttgccgatcg gaaacgggat gctgctttac ggaagcaggg
     4861 gctggcggtg cttcgtttta cggacagaga ggtgctcacc aacaccgatg gggtgatgca
     4921 cgctatttat gagcagattc aaaagcgatt gcgagcgtca tgacgctgct cagcatctct
     4981 tttaagaaga ggtagttctg agtattccct ccccttcgag gggagggcga gggaggggtc
     5041 agagtatggc gtgaatcccc ccgtttgcca aatcggggct tttggcctat gttttagcag
     5101 cggagtcgct atgaatgcgg tactgacaaa ggcagtcaag acggttcaaa cgcaccacct
     5161 ggtgaagccg ggggagaagg tcgtcgtggc ctgctccggc gggccggatt cggttttcct
     5221 cgttcacctc ttcgcgcacc tcaagccgct catgtttttg gatgtggcgg tcgcccacct
     5281 gaaccactct ctgcgcgggg cggaatccga cgccgatgag cagttcactc aggacttagc
     5341 cggacaattg gaattgccgt tttacgcgcg caaggtcgat gtcgctgccc tcgcagaaaa
     5401 ggagcacctg tcgctggagg cctgcggccg gaatgagcgc tactgcttct tctccgaatt
     5461 ggccgagcaa ctgaacgctg agaaaatcgc caccggccat acggcggacg accaggcgga
     5521 aacggtcttg atgcacctgt tgcgcggggc ggggagtcgc gggttagctg gaatgccttt
     5581 gctgcggggg aaaatcatcc gcccgctcct gcacctctct aaaaaagaaa ttctcgccta
     5641 tttagagtcg caaaaaattc aataccggat cgaccggacg aatgagaaga ccgattttct
     5701 gcgcaataaa attcgacatc acctcatgcc gctcttagaa caagaataca acccctccat
     5761 caccgccgct ttggggcgaa cggctgaaat cttagaacag caggagaatt acctggagtg
     5821 gttcgcgcag gaagcggtgg aagactgtct cgtctcttcc ggcgaggagg cagtcattct
     5881 ggacagcggc cgcttcctca attacgatcc ggtgatccaa cgtttggtcg tccgctcggt
     5941 ctggcaaaag ttgaccggct cagcattcgg cctcggcttt gaaacggtgc ggcgggtgat
     6001 tgacgatgcc aacggccaat cgcgattggc acctctggga agccgcttct gggtcgagca
     6061 caccggcact cagttgatct tcttcaagaa ggtgacgcag cgggtagagc acagcgtggt
     6121 gctccccgga tcatattcct ggggaaaccc gccgctttcg cttgtagcag aagtggtgga
     6181 gcgggaggga atcgatctct ttgtcaacgg cgatccggcg gtcgcttact tcgactggga
     6241 ccggctaacg ccgccgtttg tgctccgctc ggtcaagcca ggcgaccgct ttgcccctct
     6301 ggggatgggt ggccagaaga aggtgggcga tttcttcacc gacctcaaaa ttccccgata
     6361 ccagagagcc aaaagtctcg tgcttacgag caacggtgag gtggtctggc tcgtgggata
     6421 ccggatcagc gaccggttta aggtgaccat tcagacccgc agggtgctca aagcaatagt
     6481 ccgcgatggc ctattcgctc caaagggaaa gacccatcca gccgattata tccaacaggg
     6541 ctttacaaaa gcgggttagg gagttaggcc ggcaactgtc gcgcgactac cggacgaaaa
     6601 atccgctctg tgtggggatc ctgaagggca gtttcatttt cctggccgac ttagtccggc
     6661 atttgacgat cccgcacgaa gtcgatttca tatcggtggc gagttacggg gaggccaccc
     6721 gggcgcaggg ctcggtgcag ttgctcaaag atttgaaccg ccccgtcggc ggacgacacg
     6781 tagtaattgt agaggatatc gtggacaccg gactgaccgt ggagtacatt cgcaaaaacc
     6841 tcctggcgcg ggggccgaag agcgtcgcca ttctgacgct tctggacaaa aaaggtcgcc
     6901 gccgcgtgcc ggtgcagctg aagtacgtgg ggtttgcgat tccgaacgac tttgtggtcg
     6961 ggtacgggtt ggacctcaat gaacagcacc gcaacctgcc gtatataggg aaagttgcgg
     7021 gcgttccgtt ggattgaaag cgaggagaga gaagcgttgt tgcaaaaacc gaaaaacctg
     7081 actgaccaga tggccaacca gccgggagcg ggcgctcccc ggtcaccccg acagcaagac
     7141 cgccgccgcc tgccgccttt gcggcgcgaa gatatacgct tccggcgcat ccgcactgcg
     7201 gccttctggg tcatgttcat tttacttgtg gtctggatgg tgaaaatctt aaacgccggc
     7261 cgcggggaag tgacccgaat ttcgtacacc gagttcatgg ggcagttgga tacgaataac
     7321 attaagaccg tcaccttcgt ggataaggac ctccaggggg aattcaagga gaagttcgag
     7381 aagaaggaag gccggttcct ccgcaagtac ggccggttca agttgcacat cccgtttgac
     7441 gacccgcagc tcctggagaa attgcgcgcc aaatcggtgg agatcgccgc cgagccgcgc
     7501 ggctgggact ggggagcggc cttgctctcg atgtcgccct tcctcgtgct tttggtcttc
     7561 tggcttttga tgctgcgcca gatgcagggc gggcccaagg ggcttttctc tttcggcaag
     7621 agccgggccc ggcttttgac cgaggataaa ccgaaggtga ccttcagcga cgtggccggg
     7681 gcggacgaag ccaaagaaga actgcgcgaa atcatcgaat ttttgcggga gccggcaaaa
     7741 tttcaaaaat taggcgggaa gattccaaaa ggagcgctcc tcttgggccc gccgggcacg
     7801 ggcaaaacgc ttttggcgcg cgctgtggcc ggcgaagcgg cggtgccgtt tttctccatg
     7861 agcggctcgg atttcgtcga gatgttcgtc ggcgttggcg cttcccgcgt gcgcgacctg
     7921 ttcgaccagg gcaagcggag cgcgccctgc atcattttca tagacgaaat tgacgctgta
     7981 ggccggcacc ggggcgctgg tctgggcggc gggcacgacg aacgggagca gacgctcaac
     8041 caactcttag tggaaatgga cggttttgag tcgaacgaag gggtcatctt gctggctgcc
     8101 accaaccgtc ccgacgttct tgatcccgcc cttttgcggc ccggccgctt tgaccggcag
     8161 atcgtggtgg acagccccga cgtgcgaggc cgcgaaggaa ttctgaaagt ccacacccgt
     8221 aagattccgg tgggtgagga tgtagaattg tcggttctgg cccgcggcac tcccgggctt
     8281 tcgggcgccg acctggccaa cctggagaac gaagcggcgc tcttggcggc gcggcggaat
     8341 catcccaaag tcaccatggt ggatttcgaa gacgccaaag acaaggtgat gatgggaacc
     8401 gaaaggcgga gtctggtcat ctccgacgac gagaagcgca ccaccgccta ccacgaagcg
     8461 gggcacgctc tggtggccaa gttgatcccc ggctccgacc cggtccacaa ggtgacgatt
     8521 attccgcgcg gcctcgctct gggactcacc tcctacctgc cgtttgacga gaagcacacc
     8581 cattccaaac agtacttaga gacccgcttg gtgcacatga tgggcggccg ggtggcggag
     8641 aggttggttt tcaatcagct caccaccggc gccggcaacg acatcgagca ggccacagtc
     8701 ttagcccgca aaatggtctg cgactggggg atgagcgaga agttggggcc tttgaccttc
     8761 ggcaataaag aagaggagat tttcctgggc c
//
LOCUS       DBNGZD010000112         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1130643, whole genome shotgun sequence.
ACCESSION   DBNGZD010000112 DBNGZD010000000
VERSION     DBNGZD010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1130643"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="AC16GM_02965"
     CDS             complement(<1..501)
                     /locus_tag="AC16GM_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2922669.1"
                     /translation="MTTHAFTVDVEDWPQSTLHSHHVITDRVVQNTRRLLHILCEHNI
                     HGTFFVLGIVAETFPRIVTEIAQEDHEVGTHGWSHVPIFTQSAREFARDLKKSLATLE
                     SILGQKVIGHRAPIFSITANTFWAFQVMADCGIRYDTSVFPIRGFRYGIPEARRFPYE
                     ISTDNSH"
     gene            complement(575..1768)
                     /locus_tag="AC16GM_02970"
     CDS             complement(575..1768)
                     /locus_tag="AC16GM_02970"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR2922670.1"
                     /translation="MAKDIVVLDGARTPFGEFCGSLRSVSATELGVSAANAALQRSGI
                     KKEWIEHVVFGNVMQTSGDAIYFARHIGLKCGIPIPVPAVTVNRLCGSGLQAIIQGAQ
                     LINLGEAEFVLAGGAENMSQSPHVIRGARWGLDLGRGQMEDALWVSLVDSYNNMGMAI
                     TAENLAERYKISREQVDEYALRSHSAAAKAQSNGRFTEEIVPVEIKNRKGDVTVVDKD
                     EHVRNDTSLEVLAKLKPVFKKEGVVTAGNASGINDGAAAVILTTKEKAKELGLSYLAE
                     MKTWSYVGCDPDVMGIGPAPAIRQVLKRARLDLEQIDLIEINEAFSAQYLAVEKELDL
                     ERNKVNVNGGAIALGHPLAASGARLTLTLLYELKRRSKKYGIASLCIGGGQGIAAVFE
                     RDEAR"
     gene            complement(1910..2299)
                     /locus_tag="AC16GM_02975"
     CDS             complement(1910..2299)
                     /locus_tag="AC16GM_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922671.1"
                     /translation="MRKTGLLLPLLVVGLFSCVFGDTPKPGSADAQKKPEVKLTGEVI
                     CLSCYMDHNSKGAQHASCAQMCAKNGVPLALLEDKTENVYPLVKGHEGGNADLAPYAG
                     KRVTLTGHWLEKGGAKIFTFAEVTPAK"
BASE COUNT          491 a          694 c          647 g          553 t
ORIGIN      
        1 gtggctgttg tctgttgaga tttcgtaggg aaaacgcctg gcctccggaa taccgtaccg
       61 aaagccgcgt atggggaaaa cgctcgtatc gtaccggata ccgcagtcgg ccattacctg
      121 aaaagcccag aaagtattgg cagttataga aaaaataggc gcacggtgcc cgatgacttt
      181 ctggcccaga atagattcga gggtggcaag cgactttttc aaatctcggg cgaattccct
      241 ggccgattgg gtgaagatcg gcacgtgact ccaaccgtga gtgcccactt cgtggtcctc
      301 ctgtgctatc tcggtcacta tccggggaaa cgtctcggcg acgatgccca gaacaaaaaa
      361 agttccgtga atgttgtgtt cacacagaat atgcaataac cgtctagtat tttgcaccac
      421 ccggtcggtg atgacgtggt gagaatgtaa tgtggactgc ggccaatctt cgacatccac
      481 ggtgaaggcg tgcgtcgtca tgattgacac ccccctggcg aaagcaaaac tgtttaaggt
      541 ggcttttggg actgaaccat gtcacgctgc ggctttaccg cgcttcatcg cgctcaaaca
      601 ccgccgcaat gccctgcccg ccgccgatgc agagggaggc gataccgtac tttttactcc
      661 ggcgcttgag ttcgtagagg agcgtcaaag tcaaccgtgc accggaggcg gccagaggat
      721 ggcctaaagc gatggccccg ccgttcacgt ttactttatt gcgttctaaa tcgagttctt
      781 tctcgaccgc caggtattgc gcggagaagg cctcattgat ctcgatcaag tcgatttgtt
      841 ctaaatcaag ccgcgcccgc ttgaggacct gccggatagc cggggccggc ccaatgccca
      901 taacgtcggg gtcgcacccg acatacgacc aggtcttcat ttccgccagg taggagaggc
      961 cgagttcctt tgctttttcc ttggtggtca agattaccgc tgcggcgccg tcgttgatgc
     1021 cggaggcgtt cccggcggtc accacgccct cttttttgaa caccggtttg agtttcgcca
     1081 gcacttccag ggaggtatcg ttgcgcacat gctcgtcttt atccaccacg gtcacgtcgc
     1141 ctttgcggtt tttaatttcc accggcacga tttcttcggt aaagcggccg ttggattgcg
     1201 ccttggccgc ggcggaatgc gaacgcagcg cgtattcatc cacctgctcg cgggaaattt
     1261 tgtaccgctc ggctaagttc tccgccgtaa tcgccatccc catgttgttg taggaatcga
     1321 ccaaagacac ccagagtgca tcttccatct gtccccggcc taaatcgagc ccccagcgcg
     1381 cgccgcgaat cacgtggggc gactggctca tattctctgc gccgccggcc agaacgaact
     1441 cggcttcgcc taagttaatc aactgcgccc cctgaatgat cgcctgcagt ccggagccgc
     1501 agagacggtt caccgtcacc gcggggacgg gaataggaat cccgcacttc aagccgatgt
     1561 gtcgggcaaa gtagatcgca tcgccggagg tctgcatcac gttgccgaag acgacgtgct
     1621 caatccactc cttttttatg cccgagcgct ggagcgccgc gttggcggca ctgacgccca
     1681 attcggtggc cgaaaccgac cggagcgatc cgcagaactc gccgaacggc gtccgcgcgc
     1741 cgtccaatac cacgatgtct ttagccatat tctatccttt acatcctctc tttatctttg
     1801 ttcaacgaaa tagtagcata ccgtccgcag aagtcaagtc ccgcttcgat cggtaactga
     1861 aaaaaaacgg gtgagagcgc gactcccacc cgtggcggaa gaatgctttt tacttcgccg
     1921 gggtaacttc ggcaaaggtg aagatcttgg ctccgccttt ttccagccag tggccggtca
     1981 acgtgactcg cttgcccgca tagggagcga gatccgcatt gccgccttcg tgtcccttca
     2041 ccagcgggta aacattttcc gttttgtcct ctaaaagtgc caaggggacg ccgttcttag
     2101 cacacatttg cgcgcacgag gcgtgctggg cccccttgga gttgtggtcc atgtagcagg
     2161 agaggcagat cacctcaccg gtgagcttta cttccggttt tttctgcgcg tcagccgacc
     2221 cgggtttggg cgtgtcgccg aagacgcaag aaaagagacc gacgaccaaa agcgggagca
     2281 agagtcctgt ctttctcatc ttttcctctt cctttctttt ctcggttgtc gtgcttcgta
     2341 ccagtgctga ctaccgttaa tacgccggca cgggagaaat cgata
//
LOCUS       DBNGZD010000113         6288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1130676, whole genome shotgun sequence.
ACCESSION   DBNGZD010000113 DBNGZD010000000
VERSION     DBNGZD010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1130676"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1975
                     /locus_tag="AC16GM_02980"
     CDS             125..1975
                     /locus_tag="AC16GM_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR2922672.1"
                     /translation="MEKAPIGTAASLKPDKNSPAYLYQCFRQLQAVIARAATLREAMQ
                     AALVYLAREGVCPQSAIWALGTDGRTFVPEVSVGLPLRELLLVSEGEFNGDDQLCLLD
                     RDPLARSPIRPGDVFRLELGGRTVGFLWALPSPAVPFETVQNLLKFCQNALAARLGQS
                     LFQTAVVRNRGVGTFLGQWQKTVASPARLEKTLPQALTVIATIFPCDYLALTLRETGG
                     NSIQRYAVTPAEPGGSGLGSVLLEKGKSLPVPASPSGRVLATGETLVREGFVDAPESD
                     EERFLRALSLLTYVVLPLREKGAVTGTLLLATRQPRARLSLPHRELLSTLSGQLLTQH
                     RLVTVARRRERLLYATVEQAQLVRQATTPDELAARTCTLIAQELPVSFCRIWRVEAPQ
                     RRLWSIAFSALRNLGDEVGLAKRCDLADLPWHALACEERKIMRADQSDLESLMPEAEL
                     RRALLPGVQKALLVPIISGDRVTGLIAVGDMRSAARRPFTADDEIFLTAVANQMAFAF
                     DRGTVPGRMFPGLPTVKGVSTEAAWRQGTLSPSTFSTLYGAVANPLSAILGSVELLRL
                     KQPELNPQSEKYLQVIERGAWRIKEMVEQPTSGAEAPSVPASDHGTARRP"
     gene            2042..4168
                     /locus_tag="AC16GM_02985"
     CDS             2042..4168
                     /locus_tag="AC16GM_02985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR2922673.1"
                     /translation="MSTADLSAIGNLLGERLSTVLGCSGFGFFWRETPADGFRIICAQ
                     TPPELPERFWEIVAAETFSVRRAANQEPITWGTGGTGELSSDLQQIFQVTAVLTVPVT
                     DNGYTQAALAAIWHRAPHQFGETDLVLARLLGDFSRAELCRLRLGRELEKGNQRLTAL
                     SELATTMYSSLNHQVVLEKLLALATKLARADGGTIYLCDKEAGLLQPLMTNREPYREQ
                     IMAFTIPLGEGLSGKVAQTGVGQILNHAEKDETAVQVPDTPLEVESLISVPLLYAGDV
                     IGVITLTALGGREFQSEDLELLTIFARQAADVLEMARLYKNLEQAYQQLFTAQEQMVQ
                     TEKLRALGEMAGGVAHDFNNMLGAVLGRVQLLLTQAQDEKTRLGLEQIQELTLAGART
                     VARLQEFTRVKTDAAFTAVDLGQVIREAIEVTKPRWRDQVQESGGFIAIENRLEPVPP
                     VQGNAAELVEVFTNLISNAVDAMPAGGTLTFTSRLDGERVSVIVTDTGQGIPEEIKSK
                     IFFPFFTTKGVKSTGLGLSVSYGIISRHKGTIEVDSAPGRGTTFTLTLPLSRPSGKPQ
                     PAFRQKTEPGRPLKILMVDDDENIREIFQDLLSLDNHRVKTAASGREALEYFAAEPFD
                     VVITDLGMPGMSGWEVTAEVKKLNPQVPVLLVSGWGAQIDEKEAQAKGADLVISKPFQ
                     LNQIRAALLEVSGRATAGRQSAAMSQ"
     gene            complement(4344..>6288)
                     /locus_tag="AC16GM_02990"
     CDS             complement(4344..>6288)
                     /locus_tag="AC16GM_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922674.1"
                     /translation="DPLSTIHKEPERPETTKVQLDFSGDDPTPDRQATPTEPAERLEL
                     ESPIAKVEASSRSIGPHPQDELAAQLVENLDQKKTGSQSSRKKKSPVTNRESDIYHIS
                     NYSVGTNALEVQRGRKENAEPPLPLSVIRSDEATPRSGSDLVIQTEPPRRPRRAPEDP
                     TSGEAGDRHERYPGLKDIAYFSGSTVRFTGGVRLHSGDEIAIGDRSFILRAKGTSKLP
                     IVLAGAVAGVLALAALIYSLVGKPTPPSVLSGVVYDRASKQLVGGVGIKIAELGKTVG
                     TDELGYFRFDDLAPGTYTLEALTPDGSAIRKKVEIQSEKSGFVTLAVPARKSPLPLLG
                     SPDQPPPNLARTVPEAGRTPRASQKGNLKIAVSVPNATVRLYGKLLGVGNTTYVNVKS
                     GSHRVAISKMGYKPWQGIVDIPQGGTTTISAALESDQEGPAKSLSFDDYLKSAQKKSA
                     AGDHSGAVADYEKALNLSPNNPLALAGRGVELAETGDRAKALNDFRQAGSLFFSDRKY
                     GLAAENFSRALDIAPSDGDLLLRRAQSYFRAGQLRESREDYKALAKQAPNSAQAYAGW
                     GLCEFQFGNMKEAIKVFEKATQLAPTTAEYYGYLTMAHFESGNRDKARKYYTKFSDFA
                     NLLDKQAFEKYPTWQMVTRAFGS"
BASE COUNT         1209 a         1905 c         1915 g         1259 t
ORIGIN      
        1 aacaccgccg tctgcaccgg tccgccggct ggataaagtc ccgaaacggt cgataatcca
       61 taatagaagc ggcagggtgt cgcttcacag accaaattgg aactttcttt gccaggaggc
      121 ggacatggag aaggcgccca taggaactgc ggcttccctg aagccagata aaaattcccc
      181 ggcctacctg taccaatgct tccgacagct acaggcggtc attgctcggg cggccacgtt
      241 gcgcgaagcg atgcaggcag cgctggtcta cttagcccgg gagggcgtct gtccgcagag
      301 cgccatctgg gcgctgggaa cggacggccg caccttcgtg ccggaggtca gtgtcggact
      361 gccactgcgg gaactcctgt tagtgagcga aggagaattc aacggagatg accaactctg
      421 tcttttggat cgagaccccc tcgcccgctc gccgatccga cccggcgacg tgtttcggct
      481 cgagctcggc ggccgaacag ttggattcct ctgggctctc cccagtcccg cagttccctt
      541 cgaaacggtt caaaatctac tcaaattctg ccagaatgcc ctggccgccc ggctggggca
      601 gtcacttttc cagacggcag tcgtccgcaa ccggggagta ggaacgttcc tcgggcagtg
      661 gcaaaaaaca gtcgcttctc cggctcgctt agagaaaacc ttgccccagg cgttaacggt
      721 catcgccacc attttcccct gcgactatct cgccctgacc ttgcgggaaa cgggcggcaa
      781 ttctatccag cgctacgcgg tgactcctgc ggagcccggg gggtccgggt tgggttccgt
      841 gctccttgag aaagggaaaa gtctccccgt accggcatcg cccagcggcc gggttttggc
      901 caccggcgaa accttggtgc gggagggttt cgtcgatgca ccggaatcgg acgaagagcg
      961 gttcctgcgc gctctgagct tgctcaccta cgtcgtactg cccctgcgcg aaaaaggtgc
     1021 ggtcaccggc acactccttc tggcaacccg tcagccgcgg gcgcggctct ccctgccaca
     1081 ccgggagctg ctcagcaccc tgagcggtca gttactcacc cagcaccgtt tggtgacggt
     1141 ggcacggcgc cgggaaaggc ttctgtacgc caccgtagaa caggcccagt tggtacgcca
     1201 agccacgacc cccgacgaac tcgccgcccg cacctgcacg ttgattgcgc aagaactgcc
     1261 ggtctccttc tgccggatct ggcgggtgga ggcaccgcaa agacggctct ggagcattgc
     1321 tttctccgcg ctgcgcaatc tgggagatga agtcggtttg gccaaaaggt gtgacttggc
     1381 cgacctcccc tggcacgccc tggcatgtga agaaaggaaa atcatgcggg ccgatcagag
     1441 cgatctggaa agtttgatgc ccgaagcgga gctccggcgg gcacttttgc cgggcgtaca
     1501 gaaagccctg ctcgtcccca ttatttcggg tgaccgcgtc accggcctga ttgccgtcgg
     1561 cgatatgcgc tcagcggccc gccgtccctt caccgccgac gacgaaatct ttttgacggc
     1621 ggtggcgaat caaatggcct tcgcctttga ccggggaacc gtcccgggaa ggatgtttcc
     1681 cggattacca acggtcaagg gggtctcgac agaggcggct tggcgccagg gaacactatc
     1741 cccgtcgacc ttttcgaccc tgtacggagc ggtggccaac ccgctctcgg ccattctcgg
     1801 ttcggtggaa ctcctgcgcc taaaacaacc ggaactcaat ccgcagagtg aaaagtacct
     1861 gcaggtcatt gaacgaggtg cctggcgcat caaggaaatg gtggagcagc caacttccgg
     1921 cgcggaagca ccgtcggtgc ccgcctcaga ccacgggacc gctcggcgac cgtaaacggt
     1981 gctttcggtt gctgcggctt cagttcgggt aacacggatg cagtcaaacg gtactcagag
     2041 aatgtcgacg gcggaccttt ccgccattgg caacctcttg ggagaacggc tttcgacggt
     2101 tttgggctgc tccggttttg gattcttctg gcgggagact ccggctgatg ggttccggat
     2161 catctgcgct cagacgccgc cggagttacc ggaacggttt tgggaaatcg tcgctgccga
     2221 aaccttttcc gtccgacgag ccgccaatca agaaccaatc acctggggca cggggggaac
     2281 gggtgaactc tccagcgacc ttcagcagat ttttcaggtc accgcggtct tgaccgttcc
     2341 cgtaacggat aacggctaca cccaggcggc tctggccgcc atctggcacc gggcgcccca
     2401 tcaattcggt gagaccgatt tggtgctggc gcgccttttg ggcgatttct cccgggcgga
     2461 actctgccgg ttacgcttgg ggcgggaact ggaaaaggga aatcagcgcc tcaccgcact
     2521 ctccgaactc gccaccacga tgtattcttc cctcaatcac caggtggtgc tggagaaact
     2581 tttggccctg gcgaccaagc tggcgcgcgc cgacggcggc accatttacc tctgcgacaa
     2641 agaagccgga ctgctccagc ccctgatgac caaccgggaa ccctaccgcg agcagatcat
     2701 ggccttcacg atacccctgg gggaaggact ctcgggaaag gtggcccaga ctggcgtcgg
     2761 ccagatcctc aatcacgcgg aaaaggacga aacggcggtg caggttcccg atacgcccct
     2821 ggaggtcgaa tcgctcattt ccgtgccgct cctgtacgcc ggcgatgtga tcggcgtcat
     2881 taccctgacc gctctgggag ggcgggagtt ccagtccgag gatttggaac tcttgaccat
     2941 tttcgcccgc caggccgccg atgttctcga aatggcccgg ctgtacaaaa atctggagca
     3001 ggcctaccag cagttgttca cggcccagga gcagatggtg cagaccgaaa agctgcgcgc
     3061 actgggagag atggccggag gagtagccca cgattttaac aacatgctcg gtgccgtcct
     3121 ggggcgggtg caactgctcc tgacgcaggc gcaggatgaa aagacccgtc tcggtctgga
     3181 gcaaattcaa gaactcaccc tggcgggggc gcggacggtc gcccgcttac aggagttcac
     3241 ccgggtgaaa accgacgccg ccttcaccgc cgttgacctg ggtcaggtca tccgcgaagc
     3301 gattgaggtg accaagcccc gctggcgcga tcaggtgcag gagagcggcg gattcattgc
     3361 gatcgaaaac cggctggagc cggtgccgcc ggttcagggc aacgccgccg aactggtgga
     3421 agtattcacc aatttgatca gcaacgccgt ggacgccatg ccggcggggg gaaccctcac
     3481 tttcacttcc cgcttggacg gtgaacgggt ttcagtcatc gtcaccgata cggggcaggg
     3541 aatacctgaa gagatcaaat cgaaaatttt ctttcctttt ttcactacca agggagtcaa
     3601 gagcaccggt ctgggactgt cggtttccta cggcatcatt tcccggcaca aagggacgat
     3661 tgaagtcgat tcggcgccgg gccgcggcac gacattcacc ctcacgctgc cactcagccg
     3721 gccttccggg aaaccgcaac cggcttttcg ccaaaagacg gagccgggac gccccctcaa
     3781 gattttgatg gtggacgatg acgaaaatat ccgggaaatc tttcaggacc tcctgtccct
     3841 ggacaaccac cgggtcaaaa ccgccgccag cggccgggaa gccctggagt actttgccgc
     3901 cgagccgttt gacgtcgtga tcaccgatct ggggatgccc gggatgtccg gctgggaagt
     3961 taccgcagag gtaaagaagc ttaacccgca ggtgccggtg ctactggttt cgggctgggg
     4021 agcgcagatt gacgaaaagg aagctcaagc caaaggagcc gacctggtca tctccaagcc
     4081 cttccagttg aaccaaatcc gcgcagccct tctcgaagtc tcaggcagag cgaccgcagg
     4141 acgacaatcc gcggcgatgt ctcaataatt ccgccgatgt ttttccgcaa ctgtaatgtc
     4201 tgtcagcagc tttcccgcgt agcgccggaa gcgaccccct cacggagaag caggccgtaa
     4261 cccggagcgg gaaacctgag gggcggacaa aaaagccgcc cttccggacg gcacgaccga
     4321 cttctctcac ggataaaaag gatttacgac ccgaacgccc gggttaccat ctgccaggtg
     4381 gggtactttt caaatgcctg cttgtcgagc aggttggcga agtcgctgaa tttggtatag
     4441 tatttccgcg ccttgtcgcg gttcccgctc tcgaagtggg ccatggtgag gtagccgtag
     4501 tactccgcgg ttgtcggggc cagctgcgtg gctttctcaa aaaccttgat cgcttctttc
     4561 atattgccga actgaaattc gcagagtccc cagccggcgt aggcctgcgc ggaattgggg
     4621 gcctgtttgg ccagggcttt gtaatcctcc cggctctcac gcagctgccc ggcccggaaa
     4681 taactctgcg cccggcggag cagcaagtca ccgtccgacg gcgcaatgtc taaagcccgg
     4741 ctgaaattct cggcggccag gccgtacttg cggtcgctga agaagagact cccggcctgc
     4801 cggaaatcgt tcagcgcttt ggcccgatct ccggtctctg cgagttcgac cccgcggcca
     4861 gccagggcca gggggttatt gggcgaaagg ttcaaggctt tctcataatc cgccacggcc
     4921 ccgctgtggt cgcccgccgc actttttttc tgagctgatt tgagatagtc atcaaaggag
     4981 agtgacttgg caggaccttc ctgatctgac tctaatgccg cgctgatggt ggtggttccg
     5041 ccctggggga tatcgacgat tccctgccag ggtttgtagc ccattttgga aatggccacc
     5101 cggtgcgagc cgcttttgac attcacgtag gtggtattgc ccacccccag aagttttccg
     5161 tagagacgga ccgtagcgtt gggaacgctc acggctattt tcaaattccc tttttgactg
     5221 gcccgcggag tccggccggc ctccggaacc gtgcgcgcca gattgggcgg cggttgatcc
     5281 ggactgccca aaagaggtaa gggagacttg cgcgcgggta ctgccagggt gacgaaaccc
     5341 gacttttcgg attggatttc cacttttttg cgaatggcgg acccatccgg ggtcagcgct
     5401 tccagcgtgt acgttccggg ggccagatca tcgaaacgga agtagcccag ttcgtcggtg
     5461 ccgacggtct tgcccagttc ggctattttg attcccaccc cccctacgag ctgcttggac
     5521 gctcgatcgt acactacgcc ggacaaaacc gaaggcgggg tcggtttccc gaccaaagaa
     5581 tagatgaggg cggccagtgc cagaactccg gctaccgccc cggccaggac gatcggcagt
     5641 ttcgatgttc ccttggcgcg caggatgaag ctgcggtcgc cgatggcgat ctcgtcgccc
     5701 gagtgcaggc ggacgccgcc ggtgaaccgc accgtcgatc cggaaaagta ggcgatgtcc
     5761 tttaatccgg gataccgctc atgccggtcg ccggcttctc ccgaggtcgg atcttccggt
     5821 gcccgccgcg ggcggcgcgg cggttcggtt tgaatcacca ggtcgcttcc ggaacgcggc
     5881 gtcgcttcgt cggagcgaat gaccgaaagg ggcagcggcg gttcggcatt ttccttgcgt
     5941 ccccgctgaa cttccagagc gttggtgcct acggagtagt tggaaatgtg ataaatgtca
     6001 ctttcgcgat ttgtgacggg cgactttttc ttgcgggagg attgtgaccc ggtttttttc
     6061 tgatcgagat tttccaccag ttgcgccgcc aactcgtcct ggggatgcgg cccgatgctg
     6121 cgcgaactcg cttcgacttt ggcgatgggc gattctaatt ccagccgttc cgccggctca
     6181 gtgggcgttg cttgccggtc aggcgtcggg tcgtccccgg aaaagtccaa ttgaaccttg
     6241 gtggtctcgg gccgctccgg ctctttatgg atggtgctca gggggtcg
//
LOCUS       DBNGZD010000114        29764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1894763, whole genome shotgun sequence.
ACCESSION   DBNGZD010000114 DBNGZD010000000
VERSION     DBNGZD010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 29764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29764
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1894763"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..787)
                     /locus_tag="AC16GM_02995"
     CDS             complement(17..787)
                     /locus_tag="AC16GM_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR2922675.1"
                     /translation="MTVAGIDIGAKTTKVLIAEDGRVLVQLLVLTGLETKQTVETAFE
                     QALQQASLSREKIARITVTGVGKQEFPLEHTEVSEVKADARGAVFLFPSARTVIDVGA
                     EEGRGIKCSQAGRVVDFTLNEKCAAGSGSFTEAMARALEVPLEELGELSRQSTQQIPM
                     NAQCAVFAESEVVSLLHAKTLKADIAKAVHDAIASRISSMARKIGIEQEVVLIGGVAK
                     NLGFIDSLKTDLSLHLLVPEEPEFVGALGAALIAQENG"
     gene            complement(800..2107)
                     /gene="bzdO"
                     /locus_tag="AC16GM_03000"
     CDS             complement(800..2107)
                     /gene="bzdO"
                     /locus_tag="AC16GM_03000"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase, bzd-type, subunit O"
                     /protein_id="HIR2922676.1"
                     /translation="MPKYKTEPLQCWQKAKEIRANYYQDYARAKEKGGLRWCGGAWSF
                     GAIPAGLGDDVFSLTGEPYAASIAWDKEFSTQCLEAVEKKGFARDLCSYMRNYWGSIL
                     LKRYAFGGEFPKPDFVWQDHICCSHAKWYQVASELEGGIPMFCIDVACGPYTELNANK
                     LDYVVGQMQDGIEWLEKITNRTFDDEKFIQAVYTECRSTALWAEICAFNKAVPAPLDE
                     KSMFSLYVLGTLMKHSPIVADFYAELRDEVADRVKRGIAALAGERCRLMSDTQPPWGF
                     LKIFRYLEEFGALSVGSLYSFGLIGMWEDQADGSWGPRKTPQQKGIEIKDRETALRIL
                     AEWNLSKPEWQHFYSPRHKSQMMLRIAQEWKVDGIMLHYNRGCEGLTLGIAENRLDLI
                     KAGFPVMTFEGNMGDEREFDEQLVTSRIDAFLENLNLKKLPHE"
     gene            complement(2125..3249)
                     /gene="bzdN"
                     /locus_tag="AC16GM_03005"
     CDS             complement(2125..3249)
                     /gene="bzdN"
                     /locus_tag="AC16GM_03005"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase, bzd-type, subunit N"
                     /protein_id="HIR2922677.1"
                     /translation="MFEVFRNWEKGRHAYAKEWKQRTGGKVLGYFCTYVPEEILYAAG
                     VLPVRILGSHEPCDLTEPHIFGMFCPFSRDCLAQGLKGRYDYLDGIMIAQSCLHIRQA
                     FTSWQKHLPVDFSYYLCMPHNLQSVRAESYLTGELLEFKKAVEKWTGKEIGAECLSRA
                     IETYNCSRRLLNQVYATRKEEHPPLTGEEAMEMVISNQVTDKSEHNRALEELARNLPN
                     RKIDRQTGTRLMLIGSENDDIPFIQMLESLGATVVIDEHCTGTRYFWNEVTPRENLVE
                     AIAQRYLQRPPCPTKDWVRRRVEHTLDLAKAYRVQGAVLIQQKFCDPHELDIPAVTSA
                     LEEKGIRTLFLEFDVTVPIGQFKTRCEAFLEMLQQEEDLF"
     gene            complement(3242..3898)
                     /locus_tag="AC16GM_03010"
     CDS             complement(3242..3898)
                     /locus_tag="AC16GM_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR2922678.1"
                     /translation="MTSSIHRAAGKRSPRSERLARLLSLAAHLIAQEGYQKASIRKVA
                     RAANVSIPSLYHYFKEKEEMLFLIQYHTFDSLVRQLKERLPPATSPEQKLYRMIENHL
                     EHFLTSMDELKVCAKELQSLSGRYYGKVLQKRQEYYQLTVEIVEELKEKFRNTKVDSR
                     LATLFLFGMLNWIHMWYRPGDGDSLKKITEQMHNLFLFGLGEIPMSRRAGRRKENRDV
                     "
     gene            4116..5138
                     /locus_tag="AC16GM_03015"
     CDS             4116..5138
                     /locus_tag="AC16GM_03015"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR2922679.1"
                     /translation="MKAAVFYEAHHPLRVEEVPLPRIKPDEILVKVAACGVCHTDLHY
                     LDHGVPTFKKPPVILGHEPSGTVAEIGVEVGNFKTGDRVLLPAVLTCGRCDYCRTGRE
                     NICSAQAMLGNNVDGAYAEYVKAPAKDAFHLPNEIPLIEGSIIADAISTPFHAVKNRA
                     RVQPGDTVVVFGCGGIGINVIQVAKAAGGIVLAVDIREKKLDWAKQFGADYVVLAQET
                     EDAAKQIRKLTGGGADVAIEAIGNPLTIQQAFSSLRTGGRLVLVGYSPEDVSLSAARI
                     MYREMEIMGSLGCRPVDYPKIIALVKLGRLKVKELVTHKLPLKEIHAAFELMRRGESL
                     RSVITF"
     gene            complement(5707..6879)
                     /locus_tag="AC16GM_03020"
     CDS             complement(5707..6879)
                     /locus_tag="AC16GM_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922680.1"
                     /translation="MRAKGSWWLAWCFGTTLVTLNAVAQTPPAPPAGSADSVVATQAV
                     PPGTLPIAQKRTGRRTPAPFFSGKLTLGTTYDNNILDYSARDVRLLDSGTQPTRFAIR
                     SAGDFIFGAGTQFDFRPVIWENRPTRLRLSSAGDLYARDGVKNKGEFGAELRQSLFRH
                     AALLTGIDYLPSFYLRNLFWRGHPLGSPAYARARFRRWTYSGAFAYDFSSRVDVQVGY
                     AFETRNYNREFNERDSKSHVVNGEIDYDLAKRLRVDLSYEFDWTLADGRHNPDPRVVD
                     ISNRAHQFAMDFFFRPVVWRHHPFQLVQRAAYQFQDYTSNRPLDRYHFGRKDHEWHLR
                     TAVSLDLTRSLEAGLAYTFAMATTNTSVTAPPPAVSLADVGDYKSHALAARVTVSF"
     gene            complement(6967..7872)
                     /locus_tag="AC16GM_03025"
     CDS             complement(6967..7872)
                     /locus_tag="AC16GM_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922681.1"
                     /translation="MRRRILFWFFVGVGLFATAVAGGAKKVRSVSITPVTGRPVAVVI
                     DEEVTSYFLCSAKEPLEISVSGPGTLEIATRLLFPKGKTEGKYTVELREGGKVLKRHE
                     TQTQVAAAKLSGRSEVPGKSRKFRYDLPEGQHRLTLAFVTGEYPEVAARFTFSVRKNK
                     GEFVAITPLSFARTATALIKEKQFTYYVATPTKPVQFRVVGPVRVQIVSRLTYDERLK
                     GTQKYTVTATEGKKLFNQFALETTKSTSVVYNDWPEVAPGKSRTFYIDVPAGEHLYDF
                     ALTGSVARAVALRFSLPHTALSNEP"
     gene            complement(8021..9160)
                     /gene="pgsW"
                     /locus_tag="AC16GM_03030"
     CDS             complement(8021..9160)
                     /gene="pgsW"
                     /locus_tag="AC16GM_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HIR2922682.1"
                     /translation="MIWRQGKVSAFTLVGLALFAILIFVLAERTAQPERQPAFTTKLK
                     AAKQALVAQQTIKEYSQTLGTAIDRENDPYVTGLIGQERTPITSDRGVVTSKILATNP
                     NFAAAFVNLLLKAKVKKNDVVACGMTGSLPGWNVAFYAACWAIGARPLVITSVGSSDW
                     GANNPALTWLDMERVLADRGIFPFRSVAASIGGGSDNGRGMSPEGRQLIREAVKRNAV
                     ELIEAPDLEANIGKRMEIFDRRAENRKIVTYVNIGGGLASLGGSQNERLIPAGLSLHL
                     AAKNFPVRGVINRMAERGLPVIHLLEVEKIAERYGLPAVVLPEPPELGEGPLFFKDTF
                     SVTSTSILTALLLMVLFVFIRLDVSHYLFRRKRGTAQPAPVGTGK"
     gene            complement(9157..9648)
                     /gene="pgsC"
                     /locus_tag="AC16GM_03035"
     CDS             complement(9157..9648)
                     /gene="pgsC"
                     /locus_tag="AC16GM_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HIR2922683.1"
                     /translation="MLTEAARLLIMQSIGLGLVVSLVFSETLGLAAGGMVVPGYAALM
                     IHHPLRIVGTILASLATFGTLKYLSNYIFIYGRRRTVLVILVGFCFGWLSRELLVLNI
                     GPIALEMQIFGLVIPGLIANWMERQGVVQTITTLVIAAVLVRLLLMVISGGDVQLGAD
                     VIL"
     gene            complement(9638..10810)
                     /gene="pgsB"
                     /locus_tag="AC16GM_03040"
     CDS             complement(9638..10810)
                     /gene="pgsB"
                     /locus_tag="AC16GM_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HIR2922684.1"
                     /translation="MWVVISLVILLSAYGFWEFRQHQKVVRTIPLRIHVNGTRGKSSV
                     TRLIAGGLRAGGLVTFAKTTGSRPRLIFPDGSETRVVRAGKANIIEQVAILRRAAACQ
                     AQVLVMECMAVQPVLQRLVEQQMIKSTAGVITNVRADHLDEMGPTLEDVAKALGGTIP
                     KNGFLFTSEKNFLPLFQAIAQRVGTTVVGIDSEGVTAEMLSGFSYLEHPENVALALAV
                     CNHLGVPAPTALAGMQTAQPDAGVLRIYRIQQGERTVDFVNAFAANDPDSYHIIRERL
                     ERILNPQARLVLLVNSRADRIQRAHQLGDLVAEWPAFRVVVTGEYTTALVNRAIRSGL
                     APQRIENYGGHTVEDIYEHLVRMADHHAAVVGIGNIVGFGEQIVNFFVNRGTEFAY"
     gene            complement(10848..13061)
                     /locus_tag="AC16GM_03045"
     CDS             complement(10848..13061)
                     /locus_tag="AC16GM_03045"
                     /inference="COORDINATES: protein motif:HMM:NF052691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPS10 domain-containing protein"
                     /protein_id="HIR2922685.1"
                     /translation="MKWSSWGGKRSGWLWAIWISLTGMVGPARGAWITNGPHHKNLRA
                     VVVSPVDSLQQWVGAFGSGVYKSTDGGLTWVNERTGLNNLYVRSLLALNDTLVFAGTN
                     DGIFRSTDGGLTWVLVQATAFSIRSLAHDPTTGALYAATFGSGLYKSTDLGQNWSPIL
                     VKDFITNDQLVHQRAVAVFGRDSLYVGGSITDIDATGGSLFKSTDGGVSWTQVQPGLA
                     IRSSTMSIAISPNQPDSSLIIGTAAKGVYKSINGGLNWLAINGAGTANPLPARPAPPD
                     SGHLQITAVAFSPSYRYAVVESTGLVYRRTLGDTTGGVGWLPATGLPGMNAGIQALTG
                     FSQSLARILSGSEGDGAFRSGDSGKTWAAENTGLLGVAAAALLFSQNHTLLLGTDFGD
                     QVWRSTDSAGSWVLADSLDNFNSVRDLEQAHVDGAPIYAALYGTGVYKSTDDGRHWLL
                     TDTATLGNHLVRPLAVVPNKPDTIFAGTGNGVYKTSNGCATWLAASSGIPLSTSIRSL
                     ALDPANPNLLLAGTDLDYLYRSTDGGTSWSHITNANGLATGLPFIRSLAFDPAAPAVV
                     YAGGDSGRIFRSMDAGATWSTRAVLTTPFSVRSIVIDPHDRSILFAATFGSGVFLSTD
                     TGGSWNQFNSGLSDLEILTVIADTATPLGLYAGTANSGVFATKFTPPPPGNKGDVTGD
                     GVVDILDVVSIIQYVVFGAPLPVPAGADVNCDTVVDIIDIVAVIQFVVFGSPFPC"
     gene            complement(13379..13870)
                     /locus_tag="AC16GM_03050"
     CDS             complement(13379..13870)
                     /locus_tag="AC16GM_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIR2922686.1"
                     /translation="MATQPCPCGYFGDPGHECFCTPPQSQKYMSKISGPLLDRIDIHI
                     EVPAVKFRELAGQPTGESSSAIRERVNRARQVQLERFKNEKAIYGNAHMQSKEIRKHW
                     LIDDKSQELLRLAMQKLGLSARAYDRVLKVSRTIADLEGTTEIQPQHVSEAVRYRSLD
                     RNL"
     gene            13935..14318
                     /locus_tag="AC16GM_03055"
     CDS             13935..14318
                     /locus_tag="AC16GM_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2922687.1"
                     /translation="MGDHIRRIRLDRGLWQRQVAAEIGVDVTTLHNWETQRNTPSLRF
                     MPRIIGFLGYNPYPTEPTQPLSAKLKTHRRQLGLSQERLAELLEVDESTVRKWEAGHV
                     NSCSKYTKIIDAFLTSQSSNCSDLQ"
     gene            complement(14513..15694)
                     /gene="argJ"
                     /locus_tag="AC16GM_03060"
     CDS             complement(14513..15694)
                     /gene="argJ"
                     /locus_tag="AC16GM_03060"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR2922688.1"
                     /translation="MRLPKGFLASGVRAGIRRKHPDLALIVAEEGATAAALFTKNRFR
                     AAPVVLSQTALRKTGGHVKGVVINAGCANAITGNQGLQAARRVRARAATLLRCSPEQI
                     FLASTGIIGVVLADAKIRAALPAAVLRLSASGLASASQAILTTDVAPKVAQANFQWAG
                     RRGRLVGLAKGAGMIHPNLATMLAFLLTDAPATPRFLKAALRKAVDQSFHTISVDGDT
                     STNDTVLLLASGGLGGPTMTSLGQAADFCRALTEVCKALALMIVRDGEGATRLLEVNI
                     CGARTEREAHLAAHAVATSPLVKTALFSGDPNWGRVLAAVGRSGARFSPRRVGLSVGD
                     LPLVSDGEPAPYRETDAARLFSKERVCVRVNLGAGKAAATRITCDLGHDYVSLNGDYR
                     T"
     gene            complement(15691..17067)
                     /gene="argH"
                     /locus_tag="AC16GM_03065"
     CDS             complement(15691..17067)
                     /gene="argH"
                     /locus_tag="AC16GM_03065"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR2922689.1"
                     /translation="MTAPRIWGGRFQESPAENLKRFNDSFSCDHVLLNEEIEGSIAWA
                     RALAGAGVLSHSEVKKIVQGLVEIRKEGYAKPPDTSAYEDVHSFVEAALGVKVGVLAG
                     KLHSGRSRNDQVATDLKLFLRKALAATREQVLDLASALVRRSFEEAGTPMPGYTHLKQ
                     AEPITFGHWCLAYVEMLLRDASRLAGAVKRGDECPLGSGALAGTPLRIDRGRLAQALG
                     FARPTANSLDAVSDRDMAVEFLFAGSLLLVHLSRLAEDVILFSSDEFGFLELPDSLAT
                     GSSRMPHKKNPDVLELVRGNAARAIGDLAGLLSLLKGLPLSYNKDLQLDKEPVFRLHR
                     TLGLALPVLTDLVEKLRLDRNRMCRMAAQDNFLATELTDILAARGIPFRQAHEIVGRR
                     YAEARRAGKTLVEHGPAGKIAAGDLAALRVEKILAKKRAWGGTAPARVKEAAQAARKR
                     IQKLRRRP"
     gene            complement(17064..18335)
                     /locus_tag="AC16GM_03070"
     CDS             complement(17064..18335)
                     /locus_tag="AC16GM_03070"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR2922690.1"
                     /translation="MKKTVKKAALAYSGGLDTSIIIPWLKENYGCEVVAVSVDVGQKE
                     EISGLAEKAYRTGASEFHLVDAREEFARDYLVSVLKAGAVYEKHYLLGTATARPLIAR
                     KQVEVALATGCDALAHGCTGKGNDQVRFELAYQALAPELTVIAPWREWAISSREAAID
                     YAAARGIPVPATKQDPYSRDRNLWHLSHEGGPLEDPASEPDPALYQLTVAPEAAPDEP
                     EKVSISFEAGVPVAVNGQQFTPAQLIESLNALGGKHGVGRADLVENRLIGIKSSGVYE
                     TPGGTILVTALPALESLTLDRDSAHEKERLALRYAELVYFGQWFSPLREALDAFVEKL
                     METVTGTVTLKLYKGTVSVLGRRSPYSLYSSHLASFNMAGYDAGDATGFIRLFGLPTR
                     GRKRVAPFVLSAKRTLRVMRNEVGSQEAGIR"
     gene            complement(18332..19567)
                     /locus_tag="AC16GM_03075"
     CDS             complement(18332..19567)
                     /locus_tag="AC16GM_03075"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR2922691.1"
                     /translation="MQTLLPAVEEGRTLLPLDESPAVVASREQTFLVRTYDRTPFHPR
                     RGKGARLFDAQGRAYWDLLAGIAVNVLGHQYSRLVKTLRAEAKNLLHVSNLFYHPAQG
                     LLAEHLVRASGLSRVFICNSGTEANEAALKFARLKHPGRPDVVALEESFHGRTLGALS
                     LTGHAAYRMPFEPLVPGVRFVPPNDIESLEKTVTEKTSAIFLEPILGEGGIIPLSSAY
                     LKSARRIADATGALLILDEVQCGLGRTGALFAFQRAGILPDMVTLAKPLGGGLPLGAV
                     LTGPALEGIVKPGQHGTTFGGNPLACRLGLVVLEEIENSGLVKNVEQLGHWLGRELLK
                     LKKRCSAIVDVRGTGLMWGIELDRDASPVARALLAQGFVVGTAQKRVIRLLPPYVVPK
                     TALRSFVKALEKVLKESER"
     gene            complement(19515..20306)
                     /gene="argB"
                     /locus_tag="AC16GM_03080"
     CDS             complement(19515..20306)
                     /gene="argB"
                     /locus_tag="AC16GM_03080"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR2922692.1"
                     /translation="MNSLSALTVVKLGGSLLEDASLRAATLSAIARRWRAGASLVLVH
                     GGGREVDLRLAQLGIPKRTHQGLRITDAETLDEVISVLAGLVNKSLVAEFCARGVVAA
                     GFSGVDGATLRAEFHPRMDEVDLGFVGRVVAANPTLLKSVLKAGMLPVVASIAMGPQG
                     FLLNLNADAAASALAVSLRARRLVFLTDVEGLLDESGRVVGKLTASEACQLLDSNVVN
                     GGMRPKLAACLEAISDGVTEVVIAGPNTYADTLARGRGGTHLAPA"
     gene            complement(20303..21322)
                     /gene="argC"
                     /locus_tag="AC16GM_03085"
     CDS             complement(20303..21322)
                     /gene="argC"
                     /locus_tag="AC16GM_03085"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR2922693.1"
                     /translation="MKRVSVVGAAGYVGAELCALLARHNGVKLVGVSSSSEGEEGDFA
                     SLHPALARREGPPVQRFQLETLLGNSPDCAFLAIPHETSAQLVPRLLAQGLTVIDLSG
                     AYRLKDVSGYPQWYEFSHPCPELLPDAVYGLTEWCDGRLPGCRLVANPGCYATSILMA
                     LKPLTPFLEFQEPLICDSKSGISGAGKRKELSYSFTELSGNFRAYSAGRHRHEPEIRQ
                     ELGLSTDTPLVFVPHLLPVIRGILSTLYVSFHKPVTVAMVSEAYHRAYVHSPFVQVHP
                     AGELPELKAVVGTPRMEIGFVLLSGGRRAVIISVLDNLLKGAASQAVQNFNHVFGFDE
                     TEGLI"
     gene            complement(21397..21891)
                     /locus_tag="AC16GM_03090"
     CDS             complement(21397..21891)
                     /locus_tag="AC16GM_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:1900079 - regulation of arginine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR2922694.1"
                     /translation="MIDHSPRVRRDAILKLVRAGPVRSQVELLKRLNQRGLRVTQPTL
                     SRDLKELGIAKTASGYVTRDDLSVLAATGTSFSAPQKREEKLNQVLREFVVAVEPAGS
                     LVVLRTPSADAQPVARAMDDAALPDVAGTLAGDDTVFVAARSRPAAQRLVRRFLAVLN
                     LPRR"
     gene            22525..22755
                     /locus_tag="AC16GM_03095"
     CDS             22525..22755
                     /locus_tag="AC16GM_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2922695.1"
                     /translation="MPSIIRFLGYDPCPAEPNQALSALLKATRRLLGMSQERLADLLG
                     ADKSTIASWEQGRNRPCEKSLQVIAAFWASQP"
     gene            complement(22901..26875)
                     /locus_tag="AC16GM_03100"
     CDS             complement(22901..26875)
                     /locus_tag="AC16GM_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013256.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 N-terminal
                     domain-containing protein"
                     /protein_id="HIR2922696.1"
                     /translation="MRKGPLCRAVLLAFLLLTPGGGKLGAQSPPPTRSKKQRLLNVVG
                     TAHLDTQWRWTIQNTIEEFVPNTFRDNFKLMDIFPEYVFSFEGAFRYMLLKEYYPQDF
                     RRLRHYIASGQWRVAGSWVDAVDVNIPAFESLVRHCLYGNGFFKRELGVSSRDIFLPD
                     CFGFGYALPAIAAHCGLKSFSTQKLTWGSSVGVPFDIGLWEGVDGSTLLAALNPGDYV
                     TQIRSDLSRDTTWLKKIDHQGDTSSLSAGYMYFGTGDVGGAPDSESVGWLDKSMKSDG
                     PITVRSTGSDELVDLVAAVDQKALPRFKGELLMTRHGVGCYTSQAAMKRWNRKNELLA
                     NAAERASVVANQIAALEYPGQGLRDTWVRFLWHQFHDDITGTSIPQAYQFSWNDEVLC
                     QNRFAGILENAVEATTPHLDTRVAGVPLVVFNPLAIDREDPVEATVIFSRGAPNAVRV
                     LNAAGKEVPSQITHRYSDSLTVLFLARVPSVGYAVFDVRPEMKSLNPQVKLKISNEIL
                     ENERYRVQVDRNGDISSIFDKLLSRELLTAPIQWQLLFDKPKRWPAWEIGYDDIMAKP
                     RAVLGMPAEIKIVEAGPARVGLEVRRQTDQSKFRTVIRLTSGSAGNRIEFDSEVDWYE
                     RETLLKAVFPLASANDSVTYDLGLGAIKRGINREKLYEVPGHQWADMTSANADFGVAI
                     LNDCKYGWDHPEKGTLRLTLIHTPSVYDAWSWVGDQRSQDNGHHQFTFALCGHAGGPS
                     AEIPWQAARLNQPLLAFQTTKHQGALGPAFSFVKVKAGDPKQKSDKGEAVPHVFVNAL
                     KRAEDTGEIIVRLRELCGTSITNVPIHFARPVLKAREVNGAEESAGGATIRRGDLVVS
                     LGPYQPKAYALTLGPQKLKLSARSYQPLALPYNLDGISTDDNRLAGDFDGKGNSLAGE
                     LLPDTLMYSGVPFVFGSKTAAALNGVSCRTQNLSLPGGPNDRLYLIAAAVGGPALGTF
                     AIDGNQREIWIQDWAEPIAQWNNRLVAGRLVEEPGEIAPAYINRQPVAWYGTHRHNAR
                     GENEAYRFTYLFLIRLDLPAGARSVTLPSNPQIKLLAATAANTGYDQIRSGQPLYDYT
                     NATLAKIAADSVNFIAQAKVSLSTPISGARIHYSLDGSDPDTLSPVYRRPFTIAATTT
                     VKARAILAGSADGYVTTAKYTKLSLREAVSVGKLLPGLRCSYYEGSWSRIPRFDTLTA
                     RVDTVLEAINIPTFARKEDYGLVLTGILSVPKDGMYEFSISSDDGSQLFIADSLVVDN
                     DGLHGAGEVSGQIALKAGYHAIKVLMFQAKGDQELSAAYQGPGVQKQRISPGILFHEV
                     GGRR"
     gene            complement(26924..28273)
                     /locus_tag="AC16GM_03105"
     CDS             complement(26924..28273)
                     /locus_tag="AC16GM_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2922697.1"
                     /translation="MHSNSSRATPNRWLASYGMLVVLLLLCLFFSLLTLADQTPVGEQ
                     AARSLFEKTFPALPKRTFVLIVCQPTAEDSLFAAALSGALHKTGFRSLRCIAGDPVTI
                     RSKLAALADSVKQPVLIATTGSSASIIASIKTQFPGLADVPVVTPPSRRWPTFLLADN
                     IRNVANQITVIAIVAIGMTVVIITAGIDLSVGSLIALSAVVVAGLISKMGGETASPGA
                     MIAAGFAGILLCGIIGAFSGLMITAFRIPPFIATLAMMQVAAGFAYLISQGRPIYQLP
                     ESFVWLGRGTDPLLKVPIAVLLMVALYAVAHILMTRTTLGRYVYAVGGNPEAARLAGI
                     RVQSILLFAYALSGMLAGLGGVVMTSQLKSGAPTYGVTYELYVIAAVVVGGTSLSGGE
                     GRVLGTLIGALVIAVIGNGMNLTNVESYTQKVVLGLVILGAVLLDKLKQRGFRIGKA"
     gene            complement(28320..>29764)
                     /locus_tag="AC16GM_03110"
     CDS             complement(28320..>29764)
                     /locus_tag="AC16GM_03110"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR2922698.1"
                     /translation="KSFPGVQALSSVSFDLGVGEIHALVGENGAGKSTLIKILTGAHP
                     ADAGAILLDGQPVSYRSPSEAQASGIVTMYQEFTLVPSLSVRANLFLGHERSRRGFID
                     FRSETSEARAIFEKLRVEIDIEAKVADLSIALQQMIEIGRALAQEARILILDEPTAAL
                     TPREVESLFRILRDLQARGIGILFVSHRLDEVFAIADRITVLRDGVAIDTRSTPDYSR
                     QELIEDMVGRSIDNEYPKAEVPLGEVRFEVRQLHGGGVSDVSFSVKRGEVVGLAGLMG
                     AGRTEVARLIFGADRKVSGAISLDGRSLSINSPREAIRQGICLLTEDRKAQGLIPKAS
                     VKENFALPNLAAWSRLGWVNSQKETSRFADRVNSLRIRVTSAEQRVEDLSGGNQQKLL
                     VARWLETDSQVFLFDEPTRGIDVGAKYEIYALIGELAAQGKVVLVISSELPELLGICD
                     RILVIREGRLVADVADVRNARQEDILSLAV"
BASE COUNT         6314 a         8928 c         8342 g         6180 t
ORIGIN      
        1 gcgtccatct ttccgcctac ccgttctcct gcgcaatcag agcggcccct aaggcgccga
       61 caaactccgg ttcttccggg acgagaaggt gcagactcag atcggttttc agggaatcga
      121 tgaagcccaa attttttgcc acaccgccga tcaagaccac ctcctgctcg atccctatct
      181 tccgggccat cgaggaaatg cggctggcga tggcatcgtg gaccgctttg gcgatgtccg
      241 ccttgagcgt tttggcgtgc aaaagcgaca ccacttccga ttcggcaaaa accgcgcact
      301 gggcgttcat ggggatttgc tgggtggact ggcgcgagag ttcgcccagt tcttccagcg
      361 gcacctccag cgcgcgggcc atggcctcgg tgaaagaacc ggatccagct gcgcattttt
      421 cattgagtgt gaagtcaacc actcttcccg cttgactgca ctttatcccc cggccttctt
      481 cggcacccac gtcgataacg gtcctggccg agggaaagag gaaaacggct ccccgggcat
      541 cggctttgac ttcacttact tcagtgtgtt ccagaggaaa ctcctgcttg ccgacgccgg
      601 tgacggtgat ccgggcaatc ttctcccggg agagcgaagc ttgctggagg gcttgttcaa
      661 aggcggtctc gaccgtctgc ttcgtctcca agcccgtcaa aaccagaagc tgaacgagaa
      721 ctcttccgtc ctctgcaatg agcactttgg tggtcttggc ccctatgtca attccggcta
      781 cggtcaccgc cgcatcctct cattcgtgtg gcaatttctt caagttcaag ttttccaaaa
      841 aggcatcgat ccgggaggtg acgagctgtt cgtcgaattc tctctcatcc cccatgttgc
      901 cctcaaaggt catcaccgga aatccggcct taatgagatc gagccggttc tccgcaatcc
      961 ctaaggtcaa cccttcgcag ccccgattgt aatgcagcat gatgccgtcc accttccatt
     1021 cctgcgcgat gcggagcatc atctggctct tgtggcgcgg ggaatagaag tgctgccact
     1081 ccggtttgga caggttccac tccgctaaaa ttctgagtgc ggtttcacgg tcttttatct
     1141 caatcccctt ctgctggggg gtcttccttg gaccccagct gccatccgcc tggtcttccc
     1201 acatgccaat caaaccaaag gaatagagcg agccgacgga gagcgcccca aattcttcca
     1261 agtacctgaa aattttgaga aaaccccagg ggggctgggt gtcggacatg agccggcacc
     1321 gctcaccggc caaagcggca atgcctcttt tcacccggtc ggcgacctca tctcttagtt
     1381 cggcatagaa gtcggccaca ataggactat gcttcatcaa cgttcccaaa acgtaaagtg
     1441 aaaacatgct cttttcatcc aggggcgccg gcacggcttt attgaaagca caaatttcag
     1501 cccagagcgc agtggagcgg cactcggtgt aaactgcctg gataaatttt tcatcatcaa
     1561 aggttctatt ggtgatcttc tccagccatt caatgccatc ctgcatctgg cccaccacgt
     1621 aatccagctt gtttgcgttc agctcggtat aagggccgca ggcgacgtca atgcagaaca
     1681 tgggaatccc gccttccaac tcgctcgcca cctggtacca tttggcgtga ctgcagcaga
     1741 tgtgatcctg ccagacgaaa tcgggtttgg gaaattcgcc gccgaacgca tagcgcttta
     1801 agaggatcga accccagtag ttgcgcatat acgagcacaa atcccgggca aacccctttt
     1861 tttcaacggc ctccagacac tgtgtagaaa actccttatc ccaggctatg gaggcggcat
     1921 acggttctcc ggtgagggag aaaacatcgt cccccaaacc ggccggaatg gcaccaaaag
     1981 accaggcgcc accgcaccag cgcagaccac ccttttcctt agcccgtgca taatcttgat
     2041 agtaattggc cctgatttct ttagccttct gccagcactg caggggttca gttttgtatt
     2101 tgggcatagc gaccctcggt tctctcagaa aaggtcttct tcctgctgca gcatctccaa
     2161 aaaagcttcg caccgggttt tgaactgccc gatgggaacc gtgacatcaa actccaaaaa
     2221 cagagtccga atgccctttt cctccagggc tgaagttacc gccggaatgt ctaactcatg
     2281 cgggtcgcag aacttctgct gaatcaaaac cgcgccctgg acccgatagg ccttcgccag
     2341 atcgagcgtg tgttcgactc tccgtcgtac ccaatctttg gtggggcagg gggggcgctg
     2401 gaggtaccgc tgtgctatcg cttcgactaa attctcccgg ggggtgactt cattccagaa
     2461 gtatctggtt ccggtgcagt gctcgtcgat gaccacggtc gcccccagcg attccagcat
     2521 ctggatgaaa gggatgtcgt cattctcact gccaatgagc atcaaccggg ttccggtctg
     2581 tcggtctatt tttctgttcg ggagattccg agccaattct tctaaagccc ggttgtgctc
     2641 ggatttgtcg gtcacctgat tggaaatgac catttccatc gcctcttccc cggtgagggg
     2701 cggatgttcc tccttccggg tggcgtagac ctggtttaac agccggcgac tgcaattgta
     2761 ggtttcaatg gcccggctga ggcattccgc accgatttct ttgccggtcc atttctcaac
     2821 tgcttttttg aattcgagaa gttcacccgt caggtagctt tcagctctca cgctttgaag
     2881 attatgaggc atgcacaaat agtagctgaa atcgaccggg aggtgcttct gccagctggt
     2941 gaacgcctga cgaatgtgca ggcaggactg ggcaatcatg atcccgtcta aataatcgta
     3001 gcgacctttc aacccctggg ccagacaatc ccgggaaaaa gggcagaaca tcccgaaaat
     3061 gtgcggctcg gtcaaatcgc acggctcgtg gctgcctaaa atcctgaccg gtaggacccc
     3121 ggcagcgtag agaatctcct cggggacgta ggtgcagaaa tagcccaaaa ctttcccgcc
     3181 ggtcctctgt ttccactctt tggcgtaggc gtgtctgcct ttttcccagt ttcggaatac
     3241 ctcaaacatc tcgattttcc ttccggcgcc cggcccggcg gctcattgga atttccccga
     3301 gcccgaagag aaagaggtta tgcatctgct cggtgatttt tttcagcgag tcaccgtccc
     3361 ccggtcggta ccacatgtgg atccagttca gcatcccaaa gagaaagagt gtggctaagc
     3421 gagaatccac cttcgtattg cggaatttct ctttgagttc ctcaacgatt tccacggtca
     3481 gttgatagta ctcctgacgc ttctgcagaa ctttcccgta ataccgtccg gacaaagatt
     3541 gcagctcttt cgcgcagacc ttcaattcat ccatagaggt gaggaaatgc tccaggtgat
     3601 tttcaatcat ccggtagagt ttctgttctg gggaggtggc cggcggcagt ctctccttca
     3661 gctgccgcac cagggaatcg aaggtgtggt attgaattaa aaacagcatc tcctcttttt
     3721 ccttgaagta gtggtacagg ctggggatac tgacgttcgc cgcccgggcc accttgcgga
     3781 tgctggcttt ctggtagccc tcctgggcaa tgaggtgggc cgccaggctg agcagccggg
     3841 ccagccgttc cgagcggggg gatctttttc ccgcagcgcg gtgaatagaa gaggtcatgg
     3901 tcgtgaactc tttattctat cgaacgttcg ataatatata cgtacgctgc gctgcggctg
     3961 tcaagggaaa aaatagaaaa gctgacagtt tagtctggat ttgcgtgacg gtcttcggga
     4021 aagcccccct tctgcttggc aatcatcgga gatctggata tactcgtgag gcggtttttg
     4081 ctcttgcacg ttctttttgt ggagaggagg acgaagtgaa ggcggcggtt ttctatgaag
     4141 cccatcaccc cctgcgggtg gaggaagttc ccctcccccg aatcaaaccg gatgaaatac
     4201 tggtgaaagt ggcggcctgt ggcgtctgtc acaccgacct gcactacctg gaccacggcg
     4261 ttcccacgtt taagaaacct ccggtgatct taggccacga gccctcgggc actgtggccg
     4321 aaattggtgt cgaagtcggc aatttcaaga ctggagatag agtgctattg cccgccgttt
     4381 tgacctgcgg ccgctgcgac tactgtcgaa cgggccggga gaacatttgc agcgctcagg
     4441 ccatgctggg gaacaatgtg gacggcgcgt atgcggaata tgtaaaggcg cccgccaaag
     4501 acgctttcca tttgccgaac gaaatcccct taattgaagg cagcatcatt gccgatgcca
     4561 tttcgacccc cttccatgcg gtcaagaatc gggcccgggt tcagcccggt gacaccgtgg
     4621 tcgtctttgg ctgtggcggt atcggcatca atgtaattca ggtggccaaa gcggccgggg
     4681 gcattgtcct ggcggtggat attcgggaaa agaaattaga ctgggcaaaa caattcgggg
     4741 ccgactacgt cgtccttgct caagaaactg aggatgcagc aaagcaaatc agaaaactga
     4801 ccggaggcgg tgcggatgtg gccattgaag ccatcggaaa tccgctgacc attcaacagg
     4861 cgttcagctc ccttcgaact ggagggagat tggtgttggt agggtattca ccagaagatg
     4921 tttccctgag cgcggcgcgc ataatgtatc gggagatgga gattatgggt tctctgggat
     4981 gccggccggt tgattacccg aaaataatcg cgttggtcaa gctgggccgg ctgaaggtga
     5041 aagagttggt aactcacaaa ttacctttga aggagatcca tgctgccttt gagcttatgc
     5101 gcagaggcga gtccttgaga tcggtcataa ccttttgagt caataaatcg cgagttcaaa
     5161 taccaatgaa aacgccgagc aattcgatcc ttatcaccta ccacccggct ttcacagcca
     5221 gaggttccga tccagtgatc gatactgttt aagggtggac gaaagtacgg taggtagatg
     5281 ggagactgga agtgccagtc ccggtaaaaa aacctgaaaa ttgtcaatgc gtttctatct
     5341 tccacgtcct taatccacaa tacatctgag gaattagggt ggaggaccgg tacagtgtca
     5401 ccggaaaggt acaaatggta gctacagaaa ttcgagatcg tcagtttacg cccggccttc
     5461 gctaattttg tcgtatcaaa ggaatatctt tccgacacct cccgcaaatg catcttcttg
     5521 gctgttacct ggcaagtctt ctcgtagtca agtttcttgg ttctctccga acttgttaac
     5581 cacctacccg atacttggcg ctggcgctct cggtcgacac cgagaaactc ctccgtctgg
     5641 ttcggctaac tccgacgcgg actgcgctcg aatctttcct gtaatattgg ggatttcgat
     5701 atcgctctaa aaggaaaccg tcactcgcgc ggccagcgcg tgactcttgt agtcgccgac
     5761 atcggccaga gataccgccg ggggcggagc cgtcactgaa gtattggttg tcgccatggc
     5821 aaaggtgtac gccaaaccgg cttccaggga gcgggtcaaa tccagactga ccgccgtccg
     5881 caggtgccac tcgtgatcct tccgaccgaa atggtaacgg tcgagggggc ggttggaggt
     5941 gtagtcttgg aattgatacg ccgcccgttg cacgagctga aacgggtggt ggcgccagac
     6001 caccggccgg aagaaaaagt ccatggcgaa ctggtgcgcc cgattggaaa tatcgaccac
     6061 ccgaggatcg ggattgtgcc gaccgtcggc caacgtccag tcaaactcat aggagagatc
     6121 cacgcgtaac cgcttggcca gatcgtaatc gatttccccg ttgacgacgt ggctcttgct
     6181 gtcccgttcg ttgaattccc ggttatagtt ccgcgtctca aaggcgtagc cgacctgaac
     6241 gtcgacccga gaggagaaat cgtaggcaaa agccccggaa taagtccagc gccggaagcg
     6301 tgcccgggcg taagcgggac ttccgagcgg gtgaccgcgc cagaacaggt tgcgcaggta
     6361 aaaggagggc aggtagtcaa tcccggtcaa aagcgccgcg tgacggaaga gggactgccg
     6421 caactccgcc ccaaactccc ccttgttctt gacgccatcg cgcgcatata ggtcgcccgc
     6481 tgacgaaaga cgcagccggg tgggacggtt ctcccagatc accgggcgga aatcaaactg
     6541 ggtaccggcc ccgaagatga aatcgccggc cgacctgatg gcaaagcggg tcggctgggt
     6601 gccggagtcg agcaaccgta cgtcccgggc cgaataatcc aaaatgttat tgtcgtaggt
     6661 ggtgcccaag gtcaacttgc ccgagaaaaa gggggccggg gtccgccggc cggtgcgttt
     6721 ttgggcaatc ggcaaagtgc cgggcggcac cgcctgcgtc gccacgaccg aatcagccga
     6781 accggccggc ggcgcagggg gagtttgcgc cactgcgttc agcgtcacca gcgtcgttcc
     6841 gaagcaccag gcgagccacc agctaccttt agctctcatg cgctcctctc ttctcgaccg
     6901 gtcccggtct cctcaggtgc gtgacctggt cgccctttca ccatttcttg agcgatacag
     6961 tcacacttac ggttcattgg aaagcgccgt gtgcggcagc gaaaaccgca acgccaccgc
     7021 cctggccaca ctgcccgtca gtgcaaaatc gtagaggtgt tcaccggcgg ggacgtcaat
     7081 ataaaacgtc cggctcttgc ccggcgccac ctccggccag tcgttgtaaa ccaccgaagt
     7141 cgatttggtg gtttccagag caaattgatt gaaaagcttc ttgccttcgg ttgcggtaac
     7201 cgtgtacttc tgcgtccctt tcaggcgctc atcgtaggtc agccggctca ctatctgcac
     7261 ccggaccggc cccaccaccc ggaactgcac gggcttagtc ggcgtcgcca cgtagtaggt
     7321 gaactgcttt tccttgatga gtgcggtggc cgtgcgggca aatgaaagcg gcgtgatcgc
     7381 tacgaactcg cccttatttt tccgaactga gaaggtgaag cgcgccgcca cttcagggta
     7441 ttctccggtc acgaatgcca acgtcagccg gtgctgacct tccggcaggt cgtagcggaa
     7501 cttgcggctc ttgccgggaa cttcggaacg accggagagc ttggccgccg ccacctgagt
     7561 ctgggtctcg tgccgtttga ggaccttacc cccttcgcgg agttccacgg tgtacttccc
     7621 ttcggttttg cctttgggga aaagcagccg cgtggctatt tccagtgttc ccggtccgct
     7681 caccgaaatt tccaaaggct cttttgccga acagaggaag taggaggtaa cctcttcgtc
     7741 gatcacgacc gccaccggtc tcccggtaac cggggtgatg ctcaccgacc ggaccttctt
     7801 ggccccaccg gcaacggcgg tggcgaaaag ccccactccg acgaaaaacc agaagagaat
     7861 tcttcggcgc atagtcatcc ttttctttgc aggtttaagg cctctatctc cgatttgatc
     7921 ctgaattttt tctcttctcc gttgactgcg tcctgtctac ggtgtcctga tagtaatttc
     7981 ccttcactgc cggggcggtc actcctctat tctgtctttc tcatttgccg gttccgaccg
     8041 gcgccggctg cgccgttccc cgttttcgcc ggaacaggta gtgcgagacg tcgagtcgga
     8101 tgaaaacgaa aaggaccatc aacaacaacg ccgtaagaat cgaagttgag gtgaccgaaa
     8161 aggtgtcttt gaagaagagt ggcccttcac ccagctccgg gggttcgggg agcaccaccg
     8221 ccggcaggcc gtagcgctcc gctatttttt cgacttcgag gaggtgaatc acgggcaggc
     8281 cccgctccgc catccggttg atcacgccgc gcaccgggaa atttttcgct gccaggtgca
     8341 gggacaaacc ggccggtatg agccgctcgt tctggctccc tcccagactg gccagtccgc
     8401 cgccgatatt gacgtacgtg acgattttcc ggttctccgc cctccggtca aaaatttcca
     8461 tccttttgcc gatgttggct tccaggtcgg gcgcttcaat caattcgacg gcattgcgct
     8521 tgaccgcctc gcggatcaat tgccgcccct ccggcgacat cccccggccg ttgtcggagc
     8581 cgccgccgat ggaagcggcc acggagcgga agggaaaaat tccccggtcg gccaaaaccc
     8641 gctccatatc cagccaggtc aaagccggat tgttggcacc ccagtcggaa gaaccgacgg
     8701 aggtgatgac gagcggccgg gccccgatcg cccagcaggc ggcgtagaag gcgacgttcc
     8761 agccgggcag cgacccggtc atgccgcagg cgacgacgtc atttttcttg actttcgcct
     8821 tgaggagcag gttcacgaac gccgcggcaa aattcgggtt ggtggccaaa attttcgacg
     8881 tgaccacccc gcgatccgag gtgatcggcg tccgttcctg gccgatcaag cccgtgacat
     8941 agggatcatt ttctcggtcg atggcggtac cgagagtttg gctgtattcc tttatcgttt
     9001 gctgagccac cagcgcctgc ttggccgcct tcagtttggt tgtgaaagcg ggctggcgct
     9061 cgggttgggc cgtccgttcc gccaacacga aaatcagaat cgcaaagagt gccagtccga
     9121 cgagcgtaaa tgcagaaact ttcccctgac gccagatcat aaaatcacgt ccgcccccaa
     9181 ctgcacgtcg ccgccggaga tgaccattaa gagcaggcgc accaggaccg cggcaatgac
     9241 cagggtggtg atcgtctgca cgaccccctg ccgttccatc cagttggcaa tgagtccggg
     9301 gatgaccaga ccgaaaatct gcatttccaa agcaatcggg ccaatgttta gaacgagcag
     9361 ttcccgcgag agccagccga agcagaagcc caccaggatg accaagacgg tgcgccgccg
     9421 gccgtagatg aagatgtagt tggaaaggta cttgagcgtt ccgaaggtgg ccagactggc
     9481 cagaatcgtc cccacgatgc gcaaagggtg gtggatcatg agcgccgcgt agccgggcac
     9541 caccatcccg cccgcggcca accccagggt ttcggagaaa accaggctca ccaccagccc
     9601 cagtccgatc gactgcatga tcaaaaggcg cgccgcttca gtaagcaaat tcggtccccc
     9661 ggttgacgaa gaagttcacg atctgttcgc cgaacccgac gatgtttcca atccccacta
     9721 ccgcggcgtg gtggtcggcc atgcgcacca ggtgctcgta aatgtcctcc accgtatggc
     9781 cgccgtagtt ttcaatccgc tgcggcgcca ggccggaacg aatggcccgg ttgaccagcg
     9841 cggtggtgta ttcgccggtg accaccacgc ggaaggccgg ccactccgca accaggtcgc
     9901 ccaactggtg agcccgttga atgcggtcgg cccgggagtt caccaggagg acgagccgcg
     9961 cctgcggatt gagaatccgc tccaggcgct cccggatgat gtggtaggaa tcggggtcgt
    10021 tggccgcaaa agcgttgacg aaatcgaccg tccgctcccc ctgctggatc cggtagatcc
    10081 gcaacacgcc ggcgtccggc tgcgccgtct gcatcccggc caaggccgtc ggcgccggga
    10141 cacccaggtg gttgcagacc gccaaggcca aagccacgtt ctccgggtgt tccaggtagg
    10201 aaaaaccaga gagcatctcc gcggtgacac cctcgctatc gatccccaca acggtagtgc
    10261 ccactctctg cgcgatggct tggaacaacg ggagaaagtt tttctcggag gtgaagagga
    10321 acccgttttt cggaatcgtt ccccccaatg ccttcgcgac gtcctccagc gttggtccca
    10381 tctcatctaa atggtcggcg cggacgttgg taatgacccc ggccgtcgat ttgatcattt
    10441 gctgttccac caatcgctgc aaaaccggtt gcaccgccat gcactccatc accaggacct
    10501 gcgcctggca ggccgccgcc cgccttagga tggctacctg ctcgataatg ttcgctttgc
    10561 ccgcgcgcac cactcgcgtc tcggagccat ccgggaagat aagccgtggg cggctgccgg
    10621 tagtcttggc aaacgtgact aggccgcccg cccgcaaccc ccctgcaatg agccgggtca
    10681 ccgaggattt cccccgcgtt ccattgacgt ggatgcgcaa gggaatggta cgcacgactt
    10741 tctggtgttg ccgaaactcc cagaaaccgt aggccgaaag aagaatgacg agactaatga
    10801 ctacccacat ctctgttcta ttcccagaga gagattcggc cgctgggcta acatggaaac
    10861 gggctcccga acacgacgaa ttggatcact gcgacaatat caatgatgtc cactaccgtg
    10921 tcgcaattga catcggcgcc ggctggaacc ggcagcggag ccccaaacac gacatactga
    10981 attatcgaca ccacatcaag gatatcgacc acgccatcgc cggtgacatc ccccttattc
    11041 cccgggggtg ggggggtaaa tttggtggcg aagactcccg agttcgccgt gccggcgtag
    11101 agacccaaag gcgtcgccgt gtcggcaatg accgtcaaaa tctccaaatc gctcagacct
    11161 gagttgaact gattccagct tccgccggtg tcggtgctga ggaaaactcc ggaaccgaag
    11221 gtggcggcaa agagaatgct gcggtcgtgg ggatcgatca cgatcgagcg caccgagaac
    11281 ggggtggtga gcacagcccg cgtcgaccag gtcgccccgg catccatact gcgaaagatc
    11341 ctccccgaat caccccccgc gtaaacgacc gccggggcgg cgggatcgaa agccagactg
    11401 cggataaacg gcaaaccggt agccagcccg ttcgcattgg tgatgtgtga ccaactcgta
    11461 ccgccgtcgg ttgaacgata gagataatcc aagtcagtgc ccgccagcag gaggtttggg
    11521 ttcgccggat ccaacgccag tgaccggatt gaagtggaaa gggggatacc gctacttgcc
    11581 gccagccatg tagcgcagcc attgctcgtt ttgtaaacgc cgttccccgt gcccgcgaag
    11641 atggtatccg gtttattcgg caccaccgcc aaaggcctca caagatgatt acccagggta
    11701 gcagtatcgg tcagtagcca gtgccgtcca tcgtcggtac tcttgtagac gccagtgccg
    11761 tacagagccg cgtatatagg cgccccgtct acgtgcgcct gctccaggtc gcgcaccgag
    11821 ttgaagttgt ccagcgagtc ggccagaacc caactgccag ccgaatcggt cgagcgccag
    11881 acttgatcgc cgaagtcagt gcctaagagc agggtgtgat tctgggaaaa gaggagcgcc
    11941 gcggcagcaa cgcccaaaag tcccgtattc tccgcggccc aggttttgcc cgaatcaccg
    12001 ctgcggaaag ccccatcgcc ttcggaccca gaaagaatcc gggccaagct ttgactgaat
    12061 ccggtcagcg cctggatacc ggcgttcatt ccgggcaaac ccgttgccgg aagccagccg
    12121 acgccacctg tggtgtcgcc caaggtccga cggtagacca ggccggtcga ttccaccacg
    12181 gcataacggt aggagggact gaacgctacc gcagttattt gcaagtgtcc ggaatcgggt
    12241 ggtgccggac gcgccggcag cgggttcgca gtcccggcac catttattgc cagccagttg
    12301 agcccgccgt tgatgctctt gtagacccct ttggctgccg tcccgatgat caaacttgaa
    12361 tcgggttggt tggggctaat agctatcgac atcgtactgc tgcggatagc cagtccgggc
    12421 tgcacctggg tccaggacac tcccccgtcg gtgcttttga agagcgagcc gccagtggcg
    12481 tcaatatcgg tgatcgatcc acccacgtag agggaatcgc gcccaaaaac cgccaccgcc
    12541 cgctggtgga caagttgatc gttggtgatg aaatctttga ccaaaatggg tgaccaattc
    12601 tggcctaaat cagtactctt atacaaccca ctcccgaaag tggcggcgta gagtgcgccg
    12661 gtggtcggat cgtgcgccag agagcgtatc gaaaaggccg tcgcctgcac caagacccag
    12721 gtgagtcccc cgtcggtgct ccggaaaatc ccgtcatttg ttccggcaaa gaccaaagtg
    12781 tcgttgagcg ccaagagcga ccggacatac aggttgttca ggccggtgcg ctcattcacc
    12841 caggtcagtc cgccgtcggt actcttgtag accccgctgc cgaaggcccc cacccactgc
    12901 tggagcgaat caaccggact gaccaccacc gcccggaggt ttttgtggtg cgggccgttt
    12961 gtgatccaag cacccctggc cggcccgacc atgccagtta acgaaatcca aatcgcccac
    13021 aaccatcccg accgcttacc accccacgat gaccatttca tacctgctcc tagttgaaaa
    13081 acgctttaca ttcgccgccg acaggctcat aagtcatgga accgcgtctc ccctgttgag
    13141 gttccaggca aattagacga ccgtacccct tgccgaccca ttcctccggt tgatgcacat
    13201 gcggattcgc acaagaaccg ttcacgctat acgaatttat acgctatgcg tctgcatttg
    13261 ttgtaataca actataatac aaaaagatac gattgtcttt atcagcaatg cttccccgcg
    13321 gcgttacccc tccgttcacg ctcagggtaa cccgggcctt gccgcctgct ttccacggtc
    13381 acagattgcg atccagcgac cggtaccgca ccgcctccga cacatgctgg ggttgaatct
    13441 cagtagtgcc ttcgaggtcg gcaatcgtcc gcgatacttt cagaacgcgg tcgtacgccc
    13501 gagccgagag ccccagcttc tgcatcgcca gccgcaggag ctcctgcgat ttgtcatcga
    13561 tcagccagtg tttgcggatt tccttagact gcatgtgcgc attgccgtaa atcgccttct
    13621 catttttgaa gcgctctaac tgcacctgcc tcgctcgatt cactcgttcg cggatagcag
    13681 acgaactctc acccgtgggc tgacccgcta attcacggaa tttcaccgcg ggcacttcga
    13741 tgtggatgtc gatccggtct aaaagcgggc cggaaatctt cgacatgtac ttctggcttt
    13801 gtggcggggt gcagaagcat tcgtgcccgg gatcgccaaa atagccgcag ggacagggtt
    13861 gcgttgctat cttgcaatat catcctgaaa gccaaaagcc ccccgctccc gcctatacta
    13921 agtcgttgaa cagcatggga gaccacattc gaaggatacg gttagaccgg gggctctggc
    13981 aacggcaggt ggctgccgaa atcggcgtgg atgtgacgac cctccataac tgggagacgc
    14041 aacgcaacac cccctctctg cggttcatgc cccgcataat cggcttcctt ggctataacc
    14101 cctacccgac cgaacctact cagccccttt ctgccaagct taagacccac cggagacagc
    14161 tggggttaag ccaggagcgc ctggccgaac tgcttgaggt ggacgagagt acggtaagga
    14221 agtgggaagc cggacacgta aattcctgca gtaagtacac gaaaattatt gacgcgttcc
    14281 taacttccca atcctccaat tgctcagatt tgcaatgatt ctctcgggaa gtagtactca
    14341 cagctatacc ttgtctgacc aagcaaaagt accggttcgc ctgcggcccc acgagaaaat
    14401 ttgagaggcg ataatgactt ccgcctccat accgcaacaa ttgttttcgc cagcagccag
    14461 ataactttga cctgcggcaa tgaatgttat gaagctattt tccggcaact ctctaagtcc
    14521 ggtaatcccc gttgagcgat acgtagtcgt ggcccagatc gcacgttatg cgggtggctg
    14581 cggctttgcc ggcaccgaga ttcaccctga cacaaacacg ctctttggaa aagagccggg
    14641 cggcatcggt ttcacggtag ggtgctggct caccgtcgga taccaagggc agatcaccta
    14701 ccgacaaccc gacacgacgg ggtgaaaacc gtgcgccgct gcgcccgaca gctgcgagaa
    14761 cccgtcccca gttaggatcg ccgctgaaaa gtgcggtctt cacgagcggt gatgtggcca
    14821 cggcatgagc cgccaggtgg gcttcccgct ccgttcgcgc accacaaatg ttgacctcca
    14881 gcagccgagt ggcgccctca ccgtcgcgga cgatcatcaa ggccagcgcc ttgcagacct
    14941 cagtcaaagc ccggcagaag tccgccgctt ggccaagtga cgtcatcgtg ggacctccca
    15001 gtccaccgga agccaaaagc agaaccgtat cgttcgtgga cgtgtcgcca tcgaccgaaa
    15061 tggtgtgaaa actctgatcg accgcttttc ggagagcggc cttcaagaac ctcggggtag
    15121 ccggagcatc ggttaaaagg aacgcgagca tggtagccaa gttggggtga atcatacctg
    15181 cccctttagc caagcctacc agccggcccc tccttccagc ccattgaaaa tttgcctgag
    15241 ccaccttggg ggccacatct gtggtaagaa tggcctgcga ggcggaggcg agacctgaag
    15301 cagaaagtcg cagcaccgcg gccggcagag cggctcttat cttggcgtcg gccagaacga
    15361 caccgatgat tccggtagaa gccagaaaga tctgttccgg actgcaccgc aacagtgtcg
    15421 cggctctcgc ccgaaccctg cgggctgcct ggagcccttg atttccggtg atcgcattgg
    15481 cgcaaccagc gttgataact actcctttca catgcccgcc ggtctttcgc aaggcggtct
    15541 gggaaagcac caccggagcc gcccgaaagc ggttcttcgt gaagagtgct gctgccgtgg
    15601 caccttcttc cgcgacgatc aacgctaagt ccgggtgttt tctccggatt ccggcgcgca
    15661 cgccggatgc gagaaaacct ttgggcagtc tcacggccgc ctccggagtt tctgaatccg
    15721 tttccgggct gcctgcgcgg cttccttgac acgcgcaggc gctgttccgc cccaggcccg
    15781 cttcttcgct aaaattttct cgacacgcag ggcggccaga tctcctgccg cgattttacc
    15841 cgctgggcca tgttcgacca gggtcttccc tgctcgtcgc gcctcggcat accggcggcc
    15901 aacaatttcg tgcgcctggc gaaagggaat tccccgcgcg gccaggatgt cagtcagctc
    15961 cgtggccaga aaattatctt gagcagccat acggcacatt cgattccggt cgagcctcaa
    16021 tttttctacc aggtcggtca gcaccggcaa ggccagtccg agtgtccggt gcagccggaa
    16081 aacgggctcc ttgtcgagct ggagatcctt gttgtaggag agcggcagcc ctttcaagag
    16141 cgaaagcagc ccggccaggt cgccgatcgc acgcgccgcg ttcccgcgca cgagctccag
    16201 cacatcggga ttctttttgt gcggcattcg ggacgaaccg gttgcgagcg aatcgggaag
    16261 ttccagaaag ccaaactcat cggaagaaaa aaggatcaca tcctcggcga ggcgcgagag
    16321 atgaaccagg aggagactac ccgcaaagag aaactcgacg gccatatccc ggtcagaaac
    16381 tgcatccagc gaattggccg tgggccgggc aaaacccagc gcttgtgcca atctcccccg
    16441 gtcgattctc aggggagttc cggccagggc gcccgaacca agaggacact cgtctcccct
    16501 cttcactgcc ccggccagcc ggctggcatc tcgcaggagc atttccacgt acgccaagca
    16561 ccagtggcca aaggtgatcg gctcggcctg tttcaggtgg gtgtagccgg gcatgggggt
    16621 gcctgcctcc tcgaatgaac gacgcacgag ggccgaggcg aggtccagca cctgttcgcg
    16681 ggttgcagcg agcgccttcc gcagaaaaag tttcaggtcg gttgccacct gatcgttccg
    16741 tgagcgaccg gaatggagtt ttccggccag aaccccgacc ttgaccccca gtgctgcttc
    16801 gacgaacgag tgaacgtctt cgtaggcgct cgtgtcagga ggtttggcat acccttcttt
    16861 ccgaatctcc accagcccct gaacaatctt tttgacctcc gagtgtgaaa ggactcccgc
    16921 ccccgccagc gcgcgggccc aggctatcga tccttcgatc tcttcgttga gcagaacgtg
    16981 gtcgcaagag aaagaatcgt taaaccgttt caagttctcc gcgggagatt cctgaaaccg
    17041 ccctccccag atccgaggag cggtcacctt atccctgctt cctggctgcc gacttcgttt
    17101 cgcataaccc gcagtgtcct cttggcagac aacacgaaag gggccactcg cttcctccct
    17161 ctggttggca gcccaaagag ccggatgaat cccgtcgcgt ccccggcgtc gtaacccgcc
    17221 atgttgaacg acgcgaggtg gctggaataa agcgagtacg gagaccgccg cccgagaacc
    17281 gacaccgttc ccttgtagag tttcaacgtc accgtcccgg tgaccgtctc catcaacttc
    17341 tccacgaacg catccagtgc ctctctcaag ggcgaaaacc attgtccaaa gtagacgagt
    17401 tcggcgtagc gcaaggccag ccgttctttt tcgtgggcac tgtcccggtc gagggtgagt
    17461 gattcgagcg ccgggagggc cgtcaccagg atcgtccctc ccggcgtttc atacacgccg
    17521 ctcgacttga tgccaatcaa ccggttctcg accagatcgg cgcgccccac cccgtgcttt
    17581 ccacccagcg cgttgagcga ttcgatcaat tgggccggcg taaactgctg gccattgacc
    17641 gcaacgggca ctcctgcttc aaaggagata gaaacctttt ccggttcatc cggcgccgct
    17701 tcgggagcga cggtcagctg gtacagggcc gggtcgggtt cactggcggg atcttccagc
    17761 ggcccgccct cgtgcgaaag gtgccagagg ttgcgatccc gggaatacgg atcctgcttg
    17821 gtggcgggga ccggaattcc ccgtgcggcc gcgtagtcga tcgctgcttc gcgagagctg
    17881 atcgcccact cgcgccacgg cgcaattacg gtgagttccg gagccagtgc ctggtacgcc
    17941 agctcaaaac gcacctgatc attccctttc ccggtgcagc cgtgcgccag ggcatcgcac
    18001 ccggtggcga gcgctacttc cacctgtttt cgggcaatga gcggacgtgc cgtggcggtt
    18061 cccaaaaggt agtgtttctc gtagaccgcg ccagccttaa gaactgaaac aaggtaatcc
    18121 cgggcaaact cttcgcgagc gtctacgagg tgaaattcgc tcgcaccagt gcggtaggcc
    18181 ttctcggcca gaccggatat ctcttctttt tgtcccacgt caacggagac ggccacgacc
    18241 tcgcagccgt agttctcctt gagccagggg atgatgatgg acgtatccag cccgcctgaa
    18301 tacgccaggg ccgccttctt taccgttttt ttcatcgctc gctctccttt aaaaccttct
    18361 ccagcgcttt gacgaatgag cgcaacgctg ttttgggaac cacgtacggc gggagcaacc
    18421 ggatgacgcg cttctgcgcc gttccgacga cgaaaccttg agccaggagc gcccgggcaa
    18481 ccggggaggc gtcccggtca agttcgatcc cccacatcag accggtgcca cgcacgtcaa
    18541 cgatggccga gcagcgtttt ttcagtttga gaagctcccg gcccagccag tggcccagct
    18601 gctcaacgtt cttcaccagc cccgagtttt caatctcttc gagcacgacc agacccagtc
    18661 ggcaggcgag cggattgccg ccgaacgtcg ttccgtgctg gcctggtttt acgatacctt
    18721 ccagagctgg tccggtcagt acggctccca gcggcagacc gccccccaac ggcttggcca
    18781 gcgtgaccat atcgggcagg atgcccgctc gctgaaaggc aaacagcgcc cctgtccgac
    18841 cgagcccgca ctgaacctcg tctaagatga gcagtgctcc ggttgcgtcc gcaatcctgc
    18901 gggcagactt gaggtaggca gacgaaagcg gaatgattcc cccctcaccg agaatgggct
    18961 ccaggaaaat ggctgacgtc ttttccgtca cggttttttc gagagattca atgtcgttgg
    19021 gcgggacaaa ccgtacaccc gggacgagcg gttcgaacgg catccggtac gccgcgtgtc
    19081 cggtaaggga cagtgcgccc agcgtccggc catgaaaact ttcctccagc gccaccacgt
    19141 ccggccgccc cgggtgtttg agtcgggcaa acttgagtgc ggcctcattc gcctccgtac
    19201 cgctgttgca gatgaagacg cgagacaaac cagaagctct gaccaaatgt tcggccagaa
    19261 ggccctgagc cggatggtag aaaagattag agacgtggag gagattcttg gcttcggccc
    19321 gtaaagtttt cacaagccgc gaatattggt gccccagcac gttgacggca atgcccgcca
    19381 aaaggtccca gtaggctcgg ccctgggcat cgaaaagcct ggccccttta ccccggcggg
    19441 gatggaacgg ggttcggtca tacgttcgca cgaggaaggt ttgctctcgt gatgccacga
    19501 ccgccggcga ttcgtcaagc gggagcaagg tgcgtccctc ctctaccgcg ggcaagagtg
    19561 tctgcatacg tgttgggtcc ggcaatgacg acttcggtca cgccgtcgga gatggcttcc
    19621 agacaggcag ccagtttggg tcgcatgccg ccattcacga cgtttgagtc aagcaactga
    19681 caagcttcag acgccgtcag ctttcccaca acgcgtccac tttcatccag caggccttcc
    19741 acatcagtca aaaacaccag tctgcgagcc cgcagggaaa cagccagagc cgaggcagcc
    19801 gcgtcagcat tgaggttgag cagaaacccc tgcggtccca tggcgatgga agccaccacg
    19861 gggagcatcc cggctttcaa gacgcttttg agcagcgtgg ggttagcagc aaccacccgc
    19921 cccacgaacc ccagatccac ctcgtccatt cgcggatgaa actcggcgcg cagcgtagca
    19981 ccatctacac cggagaagcc agccgcgact acaccgcggg cacagaactc agccaccagc
    20041 gacttattca ccagccccgc gagaacggag atgacttcat cgagggtttc cgcatcagta
    20101 atccgtaatc cctggtgcgt tctcttcgga attcccagct gagcaagcct caaatcgacc
    20161 tcccgcccgc ctccgtgaac gagtacgagc gatgcgcccg cacgccacct gcgggcaatc
    20221 gcggagagcg ttgccgcccg cagggatgcg tcttccagga ggctcccgcc cagcttaacg
    20281 acggtgagag cgctgagact attcagatca gcccctcggt ttcgtcaaag ccgaacacgt
    20341 ggttaaagtt ctgcacggcc tgggaagcgg cgcccttgag gaggttgtcc agaacggaaa
    20401 taatcaccgc gcgacggccg cccgaaagga gcacaaatcc gatttccatc cgcggcgtcc
    20461 cgacgaccgc tttcagctct ggtagctccc ccgccggatg tacctggacg aagggcgagt
    20521 gaacgtaggc ccggtgatac gcttccgaca ccattgcgac ggtgacgggt ttgtgaaaac
    20581 tcacatataa cgttgaaaga attccgcgga tgacgggcag gagatgagga acaaacacca
    20641 acggtgtatc ggttgagagt cccaattcct gccggatctc cggctcatgg cggtgccttc
    20701 cggcgctgta ggccctgaag ttccccgaca gctccgtgaa cgaataagaa agctcctttc
    20761 tcttgcctgc tccggagata ccactcttgg aatcgcaaat caaaggctcc tgaaattcca
    20821 gaaacggagt aaggggtttt agggccatga gaatcgatgt ggcatagcag ccgggatttg
    20881 ccaccagacg gcaacctgga agtcgcccgt cgcaccactc ggtgagaccg tagacggcgt
    20941 cggggagcag ctccgggcag ggatgggaaa actcgtacca ctgtgggtac ccgctgacgt
    21001 ccttcaggcg ataggcaccc gagagatcaa tgaccgtaag accttgggcc agaagacggg
    21061 gcaccagttg cgccgatgtc tcgtggggaa tcgccaggaa cgcacagtcg ggagaattgc
    21121 ccaagagcgt ttctaactgg aaccgctgca ccggaggccc ttcccgccgc gccagtgcgg
    21181 gatggagaga cgcaaagtct ccttcctctc cctcagaact tgaagagact ccgaccagct
    21241 tcaccccgtt gtggcgcgcc aacaatgcgc agagttcggc tcccacatag ccggccgctc
    21301 cgacaaccga gactcgtttc atcacctcat ctgcaccttt cggctctgcg ccaagctccg
    21361 catgtcgtct gtatcgggtt acctgccaga acgcggctac cgacgcggca ggttcaggac
    21421 agccaaaaaa cggcgaacga gccgctgcgc cgcaggtctg ctgcgcgccg ccacgaagac
    21481 ggtgtcgtcc ccggcgagcg tgcccgccac atcggggagc gcggcatcgt ccatcgcccg
    21541 ggccaccggc tgggcgtcgg cggaaggcgt ccgcaggacc acgagcgatc cggccggttc
    21601 tacggccaca acaaactcgc gcagtacttg gttgagcttt tcttcccgct tttgtggtgc
    21661 ggaaaaggat gtgccggtcg ccgccagaac cgagagatca tcccgggtga cgtagcccga
    21721 ggcggtctta gcgattccca gctctttcag atcccgagaa agcgtcggtt gcgtcacccg
    21781 gagcccccgc tggttcaggc gtttgaggag ctccacctgt gaacggaccg gcccggcgcg
    21841 caccagcttg aggatcgcgt cgcgccggac ccgtggtgaa tgatcaatca ttgccgtgaa
    21901 tatttattca cgccgctggt ttgtcaagaa cttttttcgg ctaattcggt agggtgggat
    21961 atcgtgaagt actgaacgcc cacagcctcc tcgcacgagg ctggctgcgg ccgttctcga
    22021 ggtccttaat ggttacgctc cggctcctca gcgcagttct cgaatgaaga agcggtggta
    22081 taagaaaacc tgtagcagaa taagctgcgg tggggcgcga tggatagggt taaaagtgtt
    22141 tgttcctgag gagaagtggc gataattcta tctgtttctt acgagagaga aaatcgcacc
    22201 ccgctggaaa tgctctgggg cacttcaacg tattccctca atgtctctca gagtcaaccc
    22261 acagctacgc ttgggacaac tggctcctta gcacctacca cctgtctttt caccgccacc
    22321 ggttccggtc cagcgagcgg tactgcaccg cctccgaaac gtgctgggac ttaccctaag
    22381 tccttgaaat gtttgggagg ctatgtttgg aagataaggt tagacccggt tttttcgcaa
    22441 cggcaagttg tcaaacagat cggggtggat gataccacca taactaactg ggagacgcag
    22501 cgaaccactc cctccctgcg gttcatgccc tccataattc ggtttttggg ctacgatccc
    22561 tgcccggcgg aacccaacca ggcactgtcg gccctactga aagccacccg aaggctgctg
    22621 ggaatgagcc aggagcgcct ggccgacctg ctgggggccg ataaaagcac gatagccagt
    22681 tgggagcagg ggaggaaccg accttgtgaa aagtctttgc aggttattgc agctttttgg
    22741 gcttcccaac cttaactgcc ccgatggcgg gacaaggggt actgcttccc ctcaaacgca
    22801 agttgcttca cccgaaaata gaagtccctg aatcatcttc atgctaagtt caagagcgcg
    22861 aatgtaaaaa gagggctact gattccgacc ggtagagcca tcatctcctg cctccgactt
    22921 cgtggaagag aatgccgggg cttatacgct gtttctgcac tccggggccc tgataggcag
    22981 cactcagttc ctggtcgcct ttggcttgaa acatcaagac tttgatagca tggtagccgg
    23041 cctttagggc aatttgcccg ctcacctctc ccgcaccgtg cagcccgtca ttatctacta
    23101 ccagagaatc ggctatgaac agttggctgc cgtcgtctga actaatcgag aattcgtaca
    23161 tgccgtcttt tggaacggag aggataccgg tcagaaccag cccgtagtcc tctttacgtg
    23221 caaatgttgg tatgttgatc gcttcgagaa ccgtgtctac ccgagcagtt aaagtatcga
    23281 accgaggaat tcttgaccat gacccctcat agtacgaaca cctgagaccc gggagcagtt
    23341 tgccgacaga gaccgcttcg cgcagggaaa gctttgtata tttggccgtc gttacgtagc
    23401 cgtcagcgct tcccgcgaga atggcccggg ccttcaccgt ggtcgtggcc gcaatagtga
    23461 acggtcgccg gtagaccgga gatagcgtgt cggggtcgct gccgtcaagc gagtaatgga
    23521 tccgagcccc tgaaatgggg gtgctcaggg aaactttcgc ttgcgcaata aaattgacgc
    23581 tatccgccgc tatcttagcc aaagtcgcgt tggtgtagtc atagagcggc tgaccggatc
    23641 ggatctggtc atagccggtg ttggcggcag tcgctgccag caattttatt tgcggattcg
    23701 acgggagagt gacggacctt gccccggccg gaagatcgag gcgtatcagg aaaagatagg
    23761 tgaaccgata ggcctcattt tcaccacggg cattgtggcg gtgggtgcca taccacgcca
    23821 ccggctgacg gttgatgtag gcaggcgcta tctccccggg ctcctcaacc aatctcccgg
    23881 cgacgagcct gttgttccat tgtgctatcg gctcggccca atcctgtatc caaatctcgc
    23941 gttggtttcc gtcaatcgca aacgttccca gtgcgggtcc gcccacggcg gcggcgataa
    24001 ggtacagacg atcgttcggc ccccccggca aactaaggtt ttgggtgcgg caactaaccc
    24061 cgttaagagc tgcagcagtc ttggagccga atacaaaagg aaccccgcta tacatgagcg
    24121 tgtctggcaa gagttcaccg gcgagcgaat ttcccttgcc gtcaaaatca ccagcgagac
    24181 gattgtcgtc ggtacttatg ccgtcaaggt tgtacggcag tgccagcggc tggtagctgc
    24241 gtgctgacaa cttgagtttt tgaggtccca aggttaaggc atacgctttc ggctgataag
    24301 ggccgagcga tacgaccaga tcacctcttc taatggttgc cccaccggcg ctctcttctg
    24361 caccgttgac ctcgcgggct ttcaacacag gccgtgcgaa atggatcggt acgttggtga
    24421 ttgacgtacc gcacagctca cgtaatctga cgatgatttc acctgtgtcc tccgcgcgct
    24481 tgagggcgtt gacgaagacg tgggggaccg cttctccctt atccgatttc tgtttcggat
    24541 cacccgcctt tactttcaca aaagaaaatg cggggccgag cgcaccctgg tgcttggttg
    24601 tctggaaggc taacaggggc tgattcaaac gtgcggcttg ccagggaatt tcagcgctgg
    24661 ggccgcccgc atgaccgcac agggcaaacg taaactggtg atgaccgtta tcctgagagc
    24721 gctggtcccc tacccatgac caggcatcgt acacgctcgg agtatgtatc agcgtgagac
    24781 gaagtgtgcc cttctccgga tgatcccaac cgtatttgca atcgttcaag atggcaacgc
    24841 caaagtcggc gtttgcggaa gtcatatccg cccactggtg gccgggaacc tcatagagtt
    24901 tctctcgatt gatgccgcgc tttattgctc cgagcccgag gtcgtaggtg acgctgtcgt
    24961 ttgcgctcgc cagtggaaaa accgctttca acagggtttc ccgttcgtac cagtcgacct
    25021 ccgaatcgaa ttcaatccga tttccggcgc tgcctgaagt aagccggata actgtgcgaa
    25081 acttggattg gtccgtctgg cgccgaactt cgagaccgac tctggcaggg cctgcttcaa
    25141 cgattttgat ctctgccggc atgcccaata ccgctctggg tttggccatg atatcgtcgt
    25201 acccgatctc ccaggctggc caccgcttgg gcttatcgaa cagaagttgc cattgaatcg
    25261 gcgcggtgag cagttcgcgg ctcaggagct tgtcgaatat cgacgaaata tcgccattcc
    25321 gatcaacctg gactctgtag cgctcgttct ccagaatttc gttggatatc ttgagcttga
    25381 cttgaggatt caatgatttc atttcagggc gaacgtcaaa aacggcgtag ccgacgctgg
    25441 ggaccctggc gaggaagaga accgtaaggc tgtcgctgta tctgtgggtg atctgtgagg
    25501 gaacttcctt tcctgccgca ttcaggacgc gcacggcgtt gggggcacca cgagagaaga
    25561 tgaccgtcgc ctcaaccggg tcttcgcgat caattgccag agggttgaac acgaccaggg
    25621 gcacccctgc gacccgcgta tcaagatggg gggtcgtcgc ctccacggca ttctccaata
    25681 tccccgcaaa acgattctga cagagaacct cgtcgttcca ggagaactgg taggcctggg
    25741 ggatgctggt gccggtgata tcatcgtgaa actggtgcca taaaaacctg acccaggtat
    25801 cccgcaggcc ctgcccgggg tactccagcg cggcaatctg gtttgcgaca acggacgcgc
    25861 gttcggcagc gttggcgagc agttcgttct tcctgttcca gcgcttcatc gccgcctgtg
    25921 aggtatagca gccgaccccg tgacgggtca tgagcaattc ccccttgaat cggggcaacg
    25981 ccttttggtc gaccgccgca accaggtcga ccaactcatc ggagccggtg ctccttaccg
    26041 ttatagggcc gtcgctcttc atcgatttat ccagccaccc aaccgattcc gaatcgggtg
    26101 ccccgcccac gtcgccggta ccgaaataca tgtacccggc ggaaagactg gaagtatctc
    26161 cctgatgatc tatcttcttc aaccaggtgg tgtctcggct caggtcggat ctaatttggg
    26221 tgacataatc gcctggattg agggccgcca gaagcgttga cccgtccacc ccttcccaca
    26281 aaccgatgtc gaacggaacg ccgaccgatg acccccaggt gagcttttga gtggaaaaac
    26341 tcttcaaccc gcagtgcgcg gctatggctg gcagcgcgta gccgaaaccg aaacagtcgg
    26401 gaagaaagat gtcgcgactg gacacaccga gctcccgctt gaaaaagccg ttgccgtaga
    26461 ggcagtgacg caccagtgat tcaaacgcgg ggatgttgac gtcgacggcg tccacccagc
    26521 tccccgccac ccgccactga ccgctggcga tgtagtgtcg cagacgccgg aaatcctgcg
    26581 gatagtactc tttaagcagc atgtagcgga aggccccctc gaaactgaac acatattcgg
    26641 gaaaaatgtc catcagtttg aaattatcgc ggaacgtatt cggcacgaat tcttcaatcg
    26701 tgttctgaat cgtccagcgc cactgggtat ccaggtgggc ggtccccacc acgttgagca
    26761 gccgctgctt tttactccgc gtcggcgggg gactctgcgc gccgagtttg ccgccgcccg
    26821 gcgtcaaaag caaaaacgct agcaggacgg cccggcacag cggtcccttt ctcatcgttt
    26881 gcatagtgga cctttcctgc tgacctgcca ggttcacctg tgcctacgcc ttcccaattc
    26941 gaaatccccg ttgcttcaac ttgtcgagta gcacggctcc caagatcacg agccccagaa
    27001 cgaccttttg agtatagctc tcgacgttcg tcagattcat cccgttcccg atcacggcga
    27061 tgaccagagc accaatgagc gttcccaata ccctgccttc gccgccgctc agactcgtcc
    27121 ccccgaccac gacggcggcg atgacgtaga gttcgtaggt gacaccgtag gtcggcgccc
    27181 cgcttttcag ttgcgacgtc atcaccacgc cgccgaggcc agccagcatc ccggagagcg
    27241 cgtaggcaaa aaggagaatc gattgcaccc ggatgccggc cagacgcgcg gcttccgggt
    27301 taccgccaac ggcatagacg taccgtccca gcgtggtgcg agtcatcaag atatgagcga
    27361 cggcataaag tgcaaccatc aacagcaccg caatcggaac cttcagcagg gggtcggttc
    27421 cccggccgag ccagacaaaa ctctccggca gttgatagat cggcctcccc tgagaaatga
    27481 ggtacgcgaa gcctgcagcc acctgcatca ttgccagcgt ggcgatgaac ggtggtatgc
    27541 ggaaggccgt gatcatgagt ccactgaacg cgccgataat cccgcagaga agtatgccgg
    27601 caaaaccggc ggcgatcatg gcgcccggcg aggccgtttc tccccccatt ttgctgatca
    27661 acccggcgac gaccacggcg ctgagcgcta tgagactccc gactgaaaga tctatccccg
    27721 cggtaatgat cacgacggtc atgccaatgg ccacgatggc gatgacggtg atctgattgg
    27781 cgacgttgcg gatgttatcg gcaaggagaa aggtcggcca ccggcgtgac ggcggggtaa
    27841 cgaccggcac gtccgccagc ccgggaaatt gagtctttat cgaggcgatg atggaggctg
    27901 acgaccctgt cgtcgcaatt agcactggct gtttgaccga atcagccaga gcggcgagct
    27961 ttgaacggat ggtcaccgga tcgccggcta tgcatcgcag cgaccgaaaa cccgtcttgt
    28021 gcagagcccc ggaaagtgcc gccgcaaaga gactatcttc tgccgtaggc tggcagacga
    28081 ttaatacaaa agtccttttg ggcagagcgg gaaacgtttt ttcaaaaaga gagcgcgccg
    28141 cctgctctcc gacgggagtc tgatcagcca gcgtgagaag ggagaagaaa aggcacagca
    28201 gtagcagcac gaccaacatg ccgtagctag ccagccagcg gttcggagtc gctcttgagg
    28261 aattgctgtg catcgggtta aggggactcg aacacctggc ttatcctgct gttgtcggct
    28321 taaactgcca gggacaggat atcttcctgt ctggcattcc tcacgtcggc tacgtcagct
    28381 accaaccgtc cttcccgaat caccagaatg cgatcgcaga tgccgagcag ttccggaagc
    28441 tcagaggaaa ttaccaacac gactttaccc tgggcggcta gttcgccaat aagggcatag
    28501 atttcgtatt tcgctcccac atctattccc cgagtcggct catcgaagag aaaaacctgc
    28561 gaatctgttt ccagccagcg cgccaccaga agtttttgtt ggttcccccc cgaaaggtcc
    28621 tcaacgcgct gttccgccga ggtcacgcga atcctcaagc tattgacacg atcggcgaag
    28681 cggctcgtct ctttttggga attcacccag cccagccttg accacgcggc gaggttcggg
    28741 agcgcaaaat tctccttaac agacgccttc ggtatgagcc cctgtgcttt gcgatcttcg
    28801 gtcaaaaggc agatgccctg ccggatggcc tcacggggag aattgatgct cagggatcgg
    28861 ccatccaggc tgatcgcgcc gctgactttg cggtcagctc caaagatcaa gcgtgccact
    28921 tcagtacgac ccgcccccat caaacctgcc aagccgacta cttcgccgcg ctttaccgaa
    28981 aatgagacgt cgcttacccc tcccccgtga agctggcgaa cttcgaaccg cacttccccc
    29041 agcggcactt ctgccttggg atattcattg tcgatcgatc ggcccaccat gtcttcgatc
    29101 aattcttgtc gagagtagtc tggcgtggaa cgcgtgtcga tcgctacgcc atctctcaag
    29161 accgttatgc ggtcggcaat cgcaaagact tcgtccagac gatggctcac gaaaagtatc
    29221 ccgattcccc gtgcttggag atcgcgtaag atgcggaaga gactttcaac ttcccggggt
    29281 gtcaaggcag cagtcggttc atccaaaatg aggattctgg cctcctgcgc cagcgcccgg
    29341 ccgatttcaa tcatctgctg cagagcgatg ctcaggtcgg ccacttttgc ctctatatcg
    29401 atctctaccc tcagcttctc aaaaatcgcc ctcgcctcgc tcgtttctga acggaaatca
    29461 atgaaccctc ggcgggaacg ttcgtgcccc aggaacaggt ttgcccgaac ggagagggat
    29521 gggacgagag tgaattcttg gtacatcgtg acaatgccgg aggcctgggc ttcggacgga
    29581 gagcggtagc tcacgggttg tccgtcaaga agtatcgccc ctgcatccgc cggatgcgcg
    29641 ccggtaagga ttttgatgag agtgctcttg cccgccccat tttcacccac cagcgcatga
    29701 atttcgccga cccccagatc aaaggagaca gaagagagcg cctgtactcc gggaaagctt
    29761 tttt
//
LOCUS       DBNGZD010000115         3231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1894776, whole genome shotgun sequence.
ACCESSION   DBNGZD010000115 DBNGZD010000000
VERSION     DBNGZD010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1894776"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1024
                     /locus_tag="AC16GM_03115"
     CDS             269..1024
                     /locus_tag="AC16GM_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922699.1"
                     /translation="MHQPMNVPLYLEWSFWAVVVATVALILSQLPPVHILIRRAKLEL
                     ELYSRILMQHKVGNPNVQFHLILTNTGGRSVKIKGIMLGIKREGKDVGTLPAQNYLQN
                     PNDKTTVLFTSFSLKSKEEWAHIVNCLNYFSRADEKKYRNAEFKLKEDIAQKRKLPEN
                     KDLLVEADSKHVDEFVRMFDEHFVWNPGEYELHISIETSDKKKDIGRDYRFTLFESDS
                     NELSRSKDDYKYGDGIYWDSGNHTGVVVQIVEA"
     gene            complement(1222..1677)
                     /locus_tag="AC16GM_03120"
     CDS             complement(1222..1677)
                     /locus_tag="AC16GM_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR2922700.1"
                     /translation="MQSKAKTVKEYLAELPPERREAIQGVREVILKNLPQGYEETMQY
                     GMIGYVVPHRLYPPGYHCDPKLPLPFACLASQKNYMAIYFMHIYGDREAEAWFRKAYQ
                     ASGKKLDMGKSCVRFKKLEDLPLEVIGKAAALTPVKKCIERYEKIRKGK"
     gene            complement(1766..1879)
                     /locus_tag="AC16GM_03125"
     tRNA            complement(1766..1879)
                     /locus_tag="AC16GM_03125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:Gln,seq:ctg)
     gene            complement(1912..2724)
                     /locus_tag="AC16GM_03130"
     CDS             complement(1912..2724)
                     /locus_tag="AC16GM_03130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2922701.1"
                     /translation="MKLKIVLTVTVMSLFICGATAGFTGAFTHLTAGRFTLYTIEFIT
                     LEGTTLYGIFERRAPVFGRIFWRGSQNLHAIALTFDDGPNEPYTSGVLDILREFRVNA
                     TFFVIGENVERFPGVVRRTAAEGHELANHTYDHEVLPLRWPGPIRNQIKRTSDLIEKT
                     TGRRPGLFRAPHGWRNPWVNRIAREEGCVPVAWTVGVWDTDRPGAEVIVQRTLKGLGN
                     GCVLLLHDSRGTERGADASQLVTALPAILQEAQRQGYRFLTLSEMVGETNKK"
     gene            complement(2721..>3231)
                     /locus_tag="AC16GM_03135"
     CDS             complement(2721..>3231)
                     /locus_tag="AC16GM_03135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2922702.1"
                     /translation="FDPRNGLALMLRAFAITKAEFPDVRLIVGGDGTFRNYYRRLVPK
                     EVAQDVHFAGPVRDERPAYYATCDVFCSPVTKASFGITLLEAMAAGKPIVATDNIGYR
                     EVLSPEEGFCVPPDDPALFARAILRLLRDENLRQEMGANGRKKALRYSWDKVAGDIAG
                     YYAEILKTR"
BASE COUNT          735 a          880 c          883 g          733 t
ORIGIN      
        1 ggcagacgcg gcccgggcgg aggcggcggg cgcggcggca aaggcggcgg gggtaaacgc
       61 cggggcggct tcaagcgctg gtagggtaat ctcaccaaat tgctgtaata aaatcgccgc
      121 agaagtgcgg cgttgagtcc attatactgg tagaaagtcc ggcaaacttg ccttgccgga
      181 ggatgaggga gaatgacccg ccattgtttg ggctgttata cggttcaata taaacaatgt
      241 taggccccaa gttcagatgg aggtatgaat gcaccagcct atgaatgttc ctctttactt
      301 ggagtggtca ttttgggcag tcgtggtagc gacagtcgcc ttaatcctct cacaactacc
      361 gcccgtccac atattaatca ggcgtgccaa gcttgaatta gagttatact cacgcattct
      421 tatgcagcac aaagttggga atccgaatgt tcagttccac cttatcctta ccaatactgg
      481 tggacgaagc gtcaaaatta aaggaataat gttaggaata aagcgggagg gaaaagatgt
      541 gggcaccctg cctgcacaga actatttaca aaatcctaac gacaaaacga ccgtgctatt
      601 tacgagtttt tctcttaagt caaaggagga gtgggcgcat atcgtgaact gtctaaatta
      661 cttttcacgc gcagacgaga aaaaatatag aaatgcagaa ttcaaattga aggaagatat
      721 tgcccaaaag agaaagctac ctgaaaacaa ggaccttctc gttgaagctg acagcaaaca
      781 cgtagacgaa ttcgttagaa tgtttgacga acacttcgtg tggaatcctg gtgaatatga
      841 attacatatc tcgatcgaga cttccgacaa aaagaaagat atcgggaggg actataggtt
      901 tactctcttt gaatcagatt cgaatgaact gtcaaggtct aaagatgatt acaaatatgg
      961 tgatggaata tactgggact ctggaaacca tactggcgtt gttgtgcaga ttgttgaggc
     1021 ctaatcagcg agtgcaggct atgcccaccg gcggtgtctg cgactgaccc ttgtccgtta
     1081 ggctggcaag ttcgacgacc gcgcgatctt gctcgcgttc gcgggcagga ttcgaaccgt
     1141 cattagaaag tcggaaatgg aattggctgg ggggacaggg atttcccggc cgtaacccga
     1201 ggactcggct cgggggaacc tctattttcc tttcctgatt ttttcgtatc gctctatgca
     1261 ttttttgacc ggggtcaacg ccgccgcctt gccgatcacc tccagcggca ggtcctccaa
     1321 tttcttgaac cggacgcacg attttcccat atccagcttc ttgccgctcg cctgatacgc
     1381 ctttctgaac caggcctccg cctcccggtc gccgtagata tgcataaagt aaatcgccat
     1441 gtaattcttt tgcgaggcga gacaggcgaa cggcagcggc agtttggggt cgcagtgata
     1501 ccccggaggg tagagccggt gcgggacgac atagccgatc atcccgtact gcatcgtttc
     1561 ctcataccct tgaggcagat ttttgagaat gacttcgcgg actccctgaa tcgcctcgcg
     1621 tctttccggc ggcagttcag ccaggtactc ttttacggtt tttgccttgc tttgcataag
     1681 ccctcaataa tgtagccggt ttgactttga ccggacaagg tttttcactg atccctgatc
     1741 tccaatccct gatctctgct ctcaaagctg ggggacaggg attcgaaccc cgattaagtg
     1801 gtccagagcc actcgtcctg tggtacgtat gacaaatgtt aagactccac ccgtagggcc
     1861 gttcgacgac ttccccagcc aagttacaag gcgtactttc cttcgtcaac tttacttctt
     1921 attggtctcc cccaccattt ctgaaagcgt cagaaacctg tacccctgtc tctgggcttc
     1981 ctgcagaata gccggcagcg ccgttactaa ttgactggca tcggcgccgc gctcggtgcc
     2041 gcggctgtcg tggagcaaaa ggacgcagcc gttgcccagc cctttcagcg tgcgctggac
     2101 gataacctcc gcgccgggac ggtcagtgtc ccacacccct accgtccagg cgacgggaac
     2161 acacccctct tctctggcga tccggttcac ccagggattt cgccagccat gcggcgcgcg
     2221 gaagagtccg ggacgccggc cggtggtctt ttcgatcaga tcggaggttc tttttatctg
     2281 attgcggata gggcccggcc agcgcaaagg gagcacctcg tggtcgtacg tatggttggc
     2341 aagctcatgc ccttccgcag ccgtccgcct gaccacgccg gggaatctct cgacgttttc
     2401 tccgatgacg aagaaagtcg cgttgacccg aaactccctc aagatgtcta aaaccccgga
     2461 agtgtacggc tcgttggggc cgtcgtcgaa ggtaagcgca atggcgtgca aattctgaga
     2521 cccccgccag aagatgcgcc cgaaaaccgg cgcccgccgc tcgaaaatac cgtaaagcgt
     2581 cgtaccttcg agggtgataa actcgatggt atagagcgtg aaacgtccgg cggtgaggtg
     2641 ggtaaatgct ccggtgaaac cggcggtggc cccacagatg aagagcgaca tcaccgttac
     2701 ggtgaggacg atcttcagtt tcatcgcgtc tttaaaatct cggcgtagta accggcgatg
     2761 tcgccggcga ccttatccca cgaataccgg agggcctttt ttcgcccgtt ggctcccatt
     2821 tcctgtctca gattttcatc ccgcaggaga cggaggattg cccgggcgaa tagggcgggg
     2881 tcgtccgggg gcacgcagaa cccctcttcc gggctcaaca cttcccgata cccgatgttg
     2941 tcggtggcca cgatcggctt gcccgcggcc atcgcctcca gaagcgtgat gccgaaagag
     3001 gccttggtga ccggagaaca gaagacgtcg caggtcgcat agtacgccgg gcgttcatcc
     3061 cgaaccggtc ccgcaaaatg gacgtcctgc gccacttcct tcggaaccag tcgccggtag
     3121 tagttccgga acgtgccgtc cccgccgacg atgagccgca cgtcggggaa ttcggctttc
     3181 gtgatcgcaa acgcccgcag catcaaagcc aggccgttcc gcggatcgaa c
//
LOCUS       DBNGZD010000116         3161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_228386, whole genome shotgun sequence.
ACCESSION   DBNGZD010000116 DBNGZD010000000
VERSION     DBNGZD010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_228386"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..854)
                     /locus_tag="AC16GM_03140"
     CDS             complement(6..854)
                     /locus_tag="AC16GM_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HIR2922703.1"
                     /translation="MYPTDAFSRRIQTERKARKYLRKRVWKNGRKFCIRCRSYGVYQV
                     VGQRYRCRRCGYTFGDWTGRWLGRLKLTAIQWLWVLKLFELECSARRLAQETGLSYPT
                     ALQACRLIRRAIVVHTAPALLQGEVEMDEAYFGGRRKGKRGRGAAGKVPVFGILERRG
                     RVQVEVVQDVTAQQLLDLALRKVRRGSIVYTDRFRGYDSLMFCGYRHLRIDHRKVFRR
                     GKVYINGLEGFWSYAKERLIKFHGVSQQYFPLYLKEMEFRYNHRKEPMFERLAQLMAN
                     PVSNLL"
     gene            944..2368
                     /locus_tag="AC16GM_03145"
     CDS             944..2368
                     /locus_tag="AC16GM_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922704.1"
                     /translation="MQKLIDTSSASFGSGLASQRKLFRVKTGQCTGRLIAIYPKTTGT
                     IVFSWSDPPYSAWAAPTNIVTDAADFPSSAWMDKDNNVYVAYTVATTFALKMVKLSYS
                     NGTWAVGAVQSIYNNDQNYHPFIFKDKIDRLWVVWSRFITASSDYRIQVKSSTNDGAT
                     WGTGPTDPGTALSSAGSSAYGQLIALGEKIYAFYTIGGTTTALRSFDISGAVWSAEQV
                     AYSGTGLSSDFHAATSADQKIGLLFNSSAGLLFKEYDGTNFSGQFTVDAAPTTTPQLV
                     YFQSTPYALFAKNIGTDQNRALYAYRNGSNFTAAQALFGDAVPFVKVFCYDQSAVAKF
                     ADRTTEAGNTAAADVFHPTSGGLVKDPQDALYVGLDVPFNQIAVLLSTTGIAGQVIFS
                     YWDGSAWVEFTPDSGAYHFATSPALVRLWPDLESVPATWRKSAVNSSVQFWIKIEVQT
                     AFTTVPVGTQITAVPNLKYLSAGG"
     gene            2550..>3161
                     /locus_tag="AC16GM_03150"
     CDS             2550..>3161
                     /locus_tag="AC16GM_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922705.1"
                     /translation="MALTNIELVKKHLRERIVPGPQVENVPVKPVGTAASQLPNTSLL
                     ANSEKVKAQEATQPTFEQVALTSAGVPLLHPEIVPDSVVVAKDSSLAQVYVENVDYAV
                     DYDAGKIKRIGAGSITEGQSVAVWYFFFRLYTKGVDYTIDYGKGKLTRLSSGAIEDGQ
                     VVLCDYSLDLAALGDDVIANAILEAEDRVTKFVDPTYLATEDQS"
BASE COUNT          698 a         1009 c          815 g          639 t
ORIGIN      
        1 ggtgattata aaagatttga cacgggattt gccattagtt gtgccaatcg ttcgaacatg
       61 ggctctttgc ggtggttgta ccggaactcc atctctttca ggtagagcgg gaaatactgt
      121 tgggagacgc catggaattt gatgagtcgc tccttggcgt agctccagaa gccttccagc
      181 ccgttgatgt acacttttcc ccggcggaag actttccggt ggtcgatccg gaggtgccgg
      241 tagccacaga acatcagact gtcgtagccc cggaaccggt cggtgtagac gatactgccg
      301 cgccgtacct tccgcaaggc caagtcgagc aactgctggg ccgtcacgtc ttggaccacc
      361 tccacctgga cgcgcccgcg ccgctccaga atgccaaaca ccgggacttt gcctgctgcg
      421 ccacggccgc gtttcccttt gcgccgacca ccgaagtaag cctcatccat ctccacctct
      481 ccctgcagca atgccggagc ggtgtgcacg acaatggccc gccggatgag ccgacaggcc
      541 tgcagggcgg tcgggtagct caaccccgtt tcctgagcca ggcgccgggc cgaacattcc
      601 aactcgaaga gtttcaacac ccagagccac tgaatcgccg tgagcttcag ccgtcccagc
      661 cagcgcccgg tccagtcccc gaaggtgtag ccacagcggc gacaacggta ccgctgcccc
      721 acaacctggt acaccccgta gctgcggcaa cgtatgcaaa atttgcgccc gtttttccac
      781 acccgttttc ggaggtactt ccgggctttc cgctccgtct gtatccgtcg agaaaacgca
      841 tccgtggggt acatgacacc agtatacttc tggtgtcaaa tcctttataa tcaccatctt
      901 ttaacctcga cacctcgata caccgacact tcgacatcgt cttatgcaga aactgattga
      961 tacgtcgagt gcctcattcg gcagcggcct ggccagccag aggaaactct tccgggtcaa
     1021 aaccggccag tgcaccgggc gcttgattgc catctacccg aagacgactg gaacgatagt
     1081 cttcagttgg tccgatccac cctactcggc ctgggccgca cccacaaaca tcgtcaccga
     1141 cgcggctgac ttcccctcct ccgcctggat ggataaagac aacaatgtgt acgtcgccta
     1201 cacggtggcc acgaccttcg ctctgaagat ggtgaagttg tcgtacagca atggcacatg
     1261 ggctgtcggc gccgttcaaa gcatttacaa taacgaccag aactaccatc ccttcatctt
     1321 caaggacaaa atcgaccggc tctgggtggt ctggtcgcgc ttcatcacgg cgtcaagcga
     1381 ctaccgcatt caggtcaaat cgtcaaccaa tgacggcgcc acctggggca caggaccaac
     1441 cgaccccgga acggcattga gcagcgccgg cagttccgcc tatggacaac tgattgctct
     1501 cggcgaaaaa atctacgcct tctacaccat cggcggcacg acgacagcgc tgcgttcctt
     1561 cgacatctcc ggcgcggtct ggagcgcgga acaggtggcc tattctggca ccggcctttc
     1621 cagcgatttc catgccgcca cctcagcgga tcagaagatc ggcctcctct tcaattccag
     1681 cgccggactt ttgttcaaag aatacgacgg cacgaacttc agcggccagt tcacagttga
     1741 cgctgcgccg acaacgactc cccaactcgt ctacttccag agcactccct acgccctctt
     1801 cgccaaaaac atcgggacgg atcaaaaccg ggcgctctac gcgtaccgaa acggaagtaa
     1861 cttcaccgcc gcgcaggcgc tcttcggaga cgcggtaccc ttcgtcaaag tcttctgcta
     1921 cgaccagagc gccgtggcga aattcgccga ccgcacaact gaagcgggaa acaccgccgc
     1981 agctgatgtg tttcatccca cctccggcgg gctcgtcaaa gacccgcagg atgcgcttta
     2041 cgtcgggctg gatgtgccct tcaaccagat tgccgttctc ctctccacca ccggcatcgc
     2101 cgggcaggtc attttcagtt actgggacgg ttccgcctgg gtggagttca ctcccgattc
     2161 aggggcgtat cacttcgcca cctcacccgc actggtgcgc ctctggcccg atttagagag
     2221 cgtgccggcc acctggcgca agagtgcggt caactccagc gtccagttct ggatcaaaat
     2281 cgaagtacag accgctttca ccaccgtccc ggtgggaacg cagataaccg ccgtcccgaa
     2341 tctcaagtac ctgagcgcag gaggatgaga gatttgaata ccaacgtagt gcaggtcgaa
     2401 gatcccgcct ctggcggggt tggtttctgt aatctgcacg ggaaagattt gaaggaattt
     2461 tccctcccct ttgaggggag ggtcagggag gggtcaaaag aatttttaac ttcgacaccc
     2521 cgacacttcg acacctcgac acttaacata tggccttaac caacatcgaa ctcgtcaaaa
     2581 aacacctccg cgagcgcatc gtccccgggc cgcaggtgga gaacgttccg gtgaaaccgg
     2641 tcggcaccgc cgccagccag ctgccgaaca cttcgctcct cgccaactcc gaaaaggtga
     2701 aagcgcagga agccacgcaa ccaacctttg aacaggttgc gctcacttcg gctggtgtgc
     2761 cactgcttca tccggaaatc gtccccgatt ccgtcgtcgt tgccaaagac agctccctgg
     2821 cgcaggtgta cgtagaaaac gtggactacg ccgtcgatta cgacgccgga aaaatcaaaa
     2881 gaattggtgc cggaagcatc accgaaggcc aatcggttgc cgtctggtat ttcttcttcc
     2941 gtctctacac caaaggagtg gattacacta tcgactacgg caaaggcaaa ctgacccgcc
     3001 tatcttcagg cgccattgaa gatggccagg tcgtcctctg cgactattca ctcgatctcg
     3061 ccgccttggg cgatgacgtg attgccaatg cgatcctcga agccgaagat cgggtgacga
     3121 aattcgtcga tccgacttat ctcgccaccg aagatcaatc g
//
LOCUS       DBNGZD010000117         7846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1687102, whole genome shotgun sequence.
ACCESSION   DBNGZD010000117 DBNGZD010000000
VERSION     DBNGZD010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1687102"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..1156)
                     /locus_tag="AC16GM_03155"
     CDS             complement(269..1156)
                     /locus_tag="AC16GM_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HIR2922706.1"
                     /translation="MRLAVVGGGPAGFFGALSAVTHNSSVQVVLFEAAQHPLRKVRIS
                     GGGRCNVTHYCFDPAELANNYPRGGKELVGPFTRFGPRETVAWFEREGVRLKVEEDGR
                     MFPVSDQSATVVDCLVQAVRASGIQLRLGTKVKHIEAVGRAAGSPQFEIARREGVNER
                     YDRVLLATGGSPAGYRFAATLGHSIVPCVPSLFTFNVADPRLEGLAGMSFEKVRLTLT
                     DGDKNRLEATGPLLITHWRFSGPAVLKLSAWGAQMLRHCNYCAELTVNLLPDYSALEL
                     YRELVAFKGQNGRKRVQSE"
     gene            1363..3261
                     /locus_tag="AC16GM_03160"
     CDS             1363..3261
                     /locus_tag="AC16GM_03160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR2922707.1"
                     /translation="MKPRKQLLRFSVFSLIWLFSSLALAQPAKQIPVRDFFKNPEKSY
                     FQISPNGKYISFTQPYEKRMNIFVQPVGSKEATRITAVTDRDISQYFWKGNDRLLYLK
                     DFGGDENFHLFAVDRTGQREKDLTPFDSVRVEVIDDLVDHDTDVLIGMNKRNREIFDV
                     YRLNTATGELALVAENPGNITGWVTDHTGGIRVATTTDGVNTSLLYRQNEKDTFRVVL
                     TTNFKESFAPLFFTFDNQNLYAASNLGRDKVAIVKYDVANGKELEVLYENPEVDVDGM
                     TYSRKRKVLTMITYVTWKPERKFLDRETEDLYKTLQKKLPKYDVFVTFNDKNEDVFTV
                     RTISDRTLGSYYLYEKKTGKLTKLADRNPWLKEGDLAEMKPVTYQSRDGLTMHGYLTL
                     PKGKEPKNLPVVVNPHGGPWARDVWGFNPEVQFLANRGYAVFQMNFRGSTGYGKKFWE
                     ISFKQWGKTMQDDVSDGVRWLVKQEIADPKRVAIYGGSYGGYCTLAGLAFSPDLYACG
                     VDYVGVSNLFTFMKSIPPYWKPYLDMFHEMVGDPQQDSALLASASPVFHVDQIKAPLL
                     VAQGAKDPRVNINESNQIVEALKSRGIDVPYMVKENEGHGFRNEENRFDFYEAMEKFL
                     AKHLLGQG"
     gene            3290..3526
                     /locus_tag="AC16GM_03165"
     CDS             3290..3526
                     /locus_tag="AC16GM_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR2922708.1"
                     /translation="MDQFCHSCAAPLSAPGFKGPAENYCKYCTDEAGNLKPKKEIQHG
                     IAQWFKSWQPNLTEQTATDRAVHYMKSMPAWAEK"
     gene            4345..4704
                     /locus_tag="AC16GM_03170"
     CDS             4345..4704
                     /locus_tag="AC16GM_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922709.1"
                     /translation="MAFERFWALPKSQWLLGRIYESYVGYLYEKDDHDVEYAGIFKGY
                     EDLGRDWICQKGNDFIVTQCNNWSQFKTIYEKQIFQFFGTIFQYRYANPGRKVQAFFI
                     QQRNYPIWLGVSLKSWD"
     gene            4842..5072
                     /locus_tag="AC16GM_03175"
     CDS             4842..5072
                     /locus_tag="AC16GM_03175"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR2922710.1"
                     /translation="MEPKKPNRYSLLRQASLLATIPAILAVGPVIGYFLGHFLDGKLS
                     TDPYLTYLLIILGFVASGREVYQIAKKANAPE"
     gene            5074..5478
                     /locus_tag="AC16GM_03180"
     CDS             5074..5478
                     /locus_tag="AC16GM_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922711.1"
                     /translation="MGLEFIQRIIKTTAVVATLAGLFVAVYYQFPYAVGLWLGALWGC
                     LNFFFIAALVKALLTPAQPIRWGNVIVTLGIKFPLLYGLGYLLLVWRYAPAVSYLIGF
                     TLLIAVTVLKALGRMLLGLDTGSLFHLTEMRK"
     gene            5479..6345
                     /gene="atpB"
                     /locus_tag="AC16GM_03185"
     CDS             5479..6345
                     /gene="atpB"
                     /locus_tag="AC16GM_03185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR2922712.1"
                     /translation="MSFTSLVHSSGLVSGGFWQEAGQPPAEVPEGAPELPNILTFVSH
                     LFGPQAAHFIHAYQNIIFAWLVMILLGVVAYLASRKSALIPGPLQNLMEMAVEELSNF
                     IIGILGPEGKRYVPFLGTIFLYTLAMNWFGLIPGLFSPTGGPNGVNITAAQAICVFLY
                     VQYTALTRLGFFKYLHHLAGEPKDAIGWSLVPINLPVHLIGELAKPFSLAIRLFGNIT
                     GEDILIAAFVGLGIALVAVLKSPVGIPLQVPFIFLALLTSTIQALVFTLLSTVYILLV
                     LPHHEEGEKREH"
     gene            6373..6606
                     /gene="atpE"
                     /locus_tag="AC16GM_03190"
     CDS             6373..6606
                     /gene="atpE"
                     /locus_tag="AC16GM_03190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR2922713.1"
                     /translation="MDFHTGLALGVPIGLGLAAIGSGIGLGGAVRGAMEAMGRQPEAS
                     GKIQTGMIIGAALCEALTIYSLIAFFMLQGRIK"
     gene            6681..7166
                     /gene="atpF"
                     /locus_tag="AC16GM_03195"
     CDS             6681..7166
                     /gene="atpF"
                     /locus_tag="AC16GM_03195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR2922714.1"
                     /translation="MNLELQQILTQIVGFLIALWILKRYAWKPLLNLLEQRRAKIRSE
                     FDAIEEQKKKIDAIAADYREKLKEIDALARVRLQEAVAEGQKVAVEIRENARREAKEL
                     LDKAKAELEREVAKAKVQLKNDLVEMTLAASRKMVKESLDTAKHRQLISDFISDLERV
                     T"
     gene            7166..7732
                     /gene="atpH"
                     /locus_tag="AC16GM_03200"
     CDS             7166..7732
                     /gene="atpH"
                     /locus_tag="AC16GM_03200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR2922715.1"
                     /translation="MARTKRTGDAALAERYAAALFALAQEKGAVEPVAADVAGLVELF
                     DRDSTLEDFLLAPHVLDEQKEELARTALSGKVLPTFLDFLLLLLDRHRFDQFRAIARR
                     YQELMEEDQGILRAQVVTVVDLPPVEALRLKAKLEQKTKKKILLKPVVDPEILGGMIV
                     KLKHQIIDRSIAQQLVELKEQLLAVKVH"
BASE COUNT         1968 a         2079 c         2113 g         1686 t
ORIGIN      
        1 caatatccag aatctccccg gctaaataca gcccgggaca gcgccggctc tgcatcgttt
       61 taaaatccac ttccttcaga ttaaccccgc cgcaggtcac aaattcttcc ttgaaaatcc
      121 cttttccgca aacggcaaag cgagctccgg tcagttcgga gacgagtgca gtcattgccc
      181 gcttggaaaa gttcgtccag gtggtttccc cggcaattcc ggcaaattga acgacccggc
      241 tccagtatcg agccggaatt gggaaaacct attcggattg aacccgcttc cggccatttt
      301 gtcccttgaa agcaaccagc tcccggtaca gctctagggc gctgtaatcg ggcagcaaat
      361 tgaccgttag ctccgcgcaa tagttgcagt gccggagcat ctgcgccccc caggccgata
      421 atttcaacac ggccggtccg ctaaatctcc agtgggtgat aagcaagggg ccggtcgctt
      481 ccagcctgtt cttatcgccg tccgtcaggg tgagccgaac cttctcaaaa ctcattcccg
      541 ccagcccctc caaacgcggg tcggccacgt taaaagtgaa aagcgaaggc acgcagggca
      601 ctatggaatg ccccaaggtc gcggcaaaac gatatccggc cggactgccg ccagtggcca
      661 aaagcacccg gtcataccgt tcgtttaccc cctcccggcg cgctatttca aattgcgggg
      721 aaccagctgc ccggccaacc gcttcgatat gcttcacttt ggtgccgagg cgcagttgga
      781 tgccggaggc gcgcaccgct tgcaccaggc aatccacgac ggttgcggat tgatcgctta
      841 ctggaaacat tcggccgtcc tcttccactt tcaaccgaac cccctctctt tcgaaccagg
      901 ccaccgtctc gcgcggtcca aagcgggtga agggccccac cagttccttg cccccccgcg
      961 ggtagttatt ggccaattcg gccgggtcaa agcagtaatg ggttacgttg cagcgcccgc
     1021 cgccggatat gcgcactttt ctcagcggat gctgcgccgc ctcgaacaac acgacttgta
     1081 ccgacgaatt atgggtaacg gcggaaagcg cgccaaaaaa tccggccggc ccccctccta
     1141 cgacagctag gcgcacaatc gcaaaccgtg cagttgaccg gacctcatac cggagtagta
     1201 tgagcgcttt ggatccaaac gacaagtgct ggagaagtgt tgacaaacgg gaggcacgac
     1261 tgtattcttg agcacaagtg ttttcgttac agagaaacaa ggaacgaccg tcagtagaga
     1321 agccacagat cagataaccg tcggcaacga aaggaaatca ccgtgaaacc gagaaaacag
     1381 ctcctccgct tcagtgtatt cagcctcatc tggcttttca gttcgctcgc cctggcccag
     1441 ccggccaagc aaatccccgt ccgggacttc ttcaaaaacc cggagaaatc ctacttccaa
     1501 atttccccga acggcaagta catctcattt acccagccct atgaaaagcg gatgaacatc
     1561 ttcgttcagc ccgtcggttc caaagaagcc acgagaatta ccgcggtcac cgaccgtgat
     1621 atttcccagt atttctggaa ggggaatgac cggctcctct acctcaagga cttcggcggc
     1681 gacgagaact tccacctctt tgcggtggac cggacaggtc aaagggagaa ggacctgacc
     1741 ccgttcgaca gcgtgcgggt ggaggtgatt gacgatctgg tggaccacga caccgacgtg
     1801 ctcatcggca tgaacaagcg gaaccgggag attttcgacg tctaccggct gaacaccgcc
     1861 accggggaac tggcactcgt cgccgaaaac cccggcaaca tcaccggctg ggtgacggat
     1921 cacaccggcg gcatccgggt ggccacgacc accgacggag tcaataccag tctcctgtac
     1981 cgccagaacg aaaaagatac ttttcgggtc gtgcttacca ccaacttcaa ggagagtttt
     2041 gcccccctgt tcttcacgtt cgataaccag aacctctacg ccgcctccaa cctgggacgg
     2101 gacaaggtcg ccatcgtcaa gtacgacgtg gccaacggca aggaactgga agtgctgtac
     2161 gagaatcccg aagtcgacgt ggatgggatg acctattccc gaaaacgcaa agtgctgacg
     2221 atgatcacct acgtcacctg gaagccggag cgaaaattct tagacagaga aactgaagac
     2281 ctgtacaaga cgttgcagaa aaagttgccg aagtacgacg tgttcgtcac gttcaacgac
     2341 aaaaacgagg atgtattcac ggtacgcact atcagcgacc gcaccctggg ttcctactac
     2401 ctctacgaga agaagaccgg caaactgacg aaactggccg accggaatcc ctggctaaag
     2461 gaaggtgact tagccgagat gaagcccgtc acctaccagt cccgcgacgg cctgacgatg
     2521 cacggctacc tgactttgcc caaagggaag gagccgaaaa acctgccggt ggtggtcaat
     2581 ccccacggcg gcccctgggc cagggacgtc tggggtttca acccggaagt ccaatttctg
     2641 gccaaccggg ggtacgccgt attccaaatg aacttccgag gttccacggg atacggaaag
     2701 aaattctggg agatttcgtt caagcaatgg ggcaagacca tgcaggatga cgtgtcggac
     2761 ggggtccggt ggctggtcaa gcaggagatt gcagatccca aacgcgtggc catttacggc
     2821 ggcagttatg gcggctactg cacgctggcc ggactggcct tttcgcccga cctgtacgcc
     2881 tgcggagtcg attacgtcgg cgtctccaac ctcttcactt tcatgaagtc gatcccgccc
     2941 tactggaaac cctacctcga catgttccac gagatggtgg gcgacccaca gcaggacagc
     3001 gcgcttctgg cctccgcctc gcccgttttc cacgtggacc agatcaaagc gccgcttttg
     3061 gtggcgcagg gtgccaaaga ccctcgcgtg aacatcaacg aatcgaatca aattgtggaa
     3121 gcgttgaaaa gccggggcat tgacgtgccc tatatggtga aggagaatga agggcacggc
     3181 tttaggaatg aggagaaccg cttcgatttt tacgaagcga tggagaagtt tttggccaag
     3241 catttactgg ggcaagggta aaaaggaagg gttcgcatca ggaggaacca tggatcaatt
     3301 ctgtcattcc tgcgctgccc ctttgagcgc acccggtttt aaaggcccgg cagagaatta
     3361 ttgcaaatac tgcaccgatg aagccggaaa tctgaaaccc aagaaagaga ttcagcacgg
     3421 cattgctcag tggtttaaaa gttggcagcc caacctcacc gagcagacgg ctacggatcg
     3481 agctgtgcat tacatgaagt ccatgccggc ttgggccgag aagtagactt agaaccggct
     3541 cgacaatttt gacggggaac ggctcataga gggggggccg gcgtctcgtg ggggggactt
     3601 gctcgcttcg gatctaaaaa cagggcgtgg gggcgaatcc cgtcctcttc accagttgga
     3661 gaaagaacgg ttataaaaat cgctgatggc ctcggtgttt ctttcgacaa tttggtaaaa
     3721 gattgaaata tgtggaaatt atttttcaaa aaatggatag tcaacttcta agcagacgga
     3781 attatatttt caatatttgg actttgggtt gttatcctta tttacgatct tgcatggatt
     3841 cctgccgcat ttattatagc acttgttgcc gtttaccaag cgtcaagtga tatgaccaaa
     3901 gaatttcaga aaaaaatatc aaaaagagct taaacaacga gaacaagcac aaaacgaaac
     3961 aaaactttgg aaacaatcgt ttattgaaaa ggccgccggt tttcgaactt tattacaggc
     4021 cagggaagaa tacgaaaaaa taaaagacaa aaaagttgag cggtttttta gatccaagag
     4081 ccgcccagcg agaaaaggag cagaggtggt acgcgaggaa gcgcaaagaa gacgagcagc
     4141 tgaataggag aataaaaaga tataagcgat catagaatac tacgaatcca ccgctccttt
     4201 tcttgtggat tcaaaaatga tattaccggt attgaagatg aggaaaaata gagagaatac
     4261 gacgaggaag aacaacttga cccagcaact cgttatttaa ccaaagtgga atttaggaaa
     4321 ccttccagtg tggaaaggaa ccaaatggct tttgaaagat tttgggctct cccaaaatcg
     4381 caatggcttc tcggcagaat ttatgaaagc tatgttggct atttatacga gaaagatgac
     4441 catgatgttg aatatgctgg gatttttaag ggctatgagg atttggggcg agattggatt
     4501 tgtcaaaaag gaaacgattt tatcgttaca caatgtaata attggtctca attcaaaact
     4561 atctacgaga aacagatttt tcagtttttc ggaaccatct ttcaataccg ctatgctaat
     4621 ccgggacgaa aagtgcaagc tttttttata caacaacgca attatccgat ctggctcggc
     4681 gtttcgctaa agagttggga ttagaactac aaaaaaattt taagtttgat aaggcctatc
     4741 cccctatcaa atgcaatgtc aggcaaaagg acttgacatg tccgagattg tgaataaatt
     4801 aacaagcgca aaacacgtca gttcacttat ggaacgtcag gttggaaccg aagaagccaa
     4861 accgatattc gctcttgcgt caggcctcgc ttttggccac gatcccggcc atcttagcgg
     4921 tgggcccggt cattggctat tttctggggc attttctgga cgggaaactt tccactgacc
     4981 cctacctgac gtacctgttg attattctgg gattcgtggc gtccgggcgg gaagtgtatc
     5041 aaatcgccaa gaaagcgaac gcgcccgagt aatatgggat tagaattcat ccaaaggatc
     5101 atcaaaacga ccgcagtggt ggccaccctc gcgggcttat ttgtggccgt gtactaccaa
     5161 tttccctacg cagtgggtct gtggctcggt gcgctctggg ggtgtctcaa tttctttttc
     5221 attgcggcgc tggtcaaggc actcctcact ccggcacaac cgattcgctg gggcaacgtg
     5281 atagtcacgc tgggcatcaa attcccgctg ctctacggtc tggggtatct tttgctcgtc
     5341 tggagatatg cgccggcggt aagttactta atcggtttca ctctcttaat cgcggtgacg
     5401 gttctcaaag ccctgggacg aatgcttctg gggctggata cagggtcgct ttttcacctc
     5461 acggagatga ggaagtaaat gtcgttcacg agcctggttc atagtagcgg tctggtgagc
     5521 ggcggctttt ggcaggaggc tggccaaccc ccggctgaag tgccggaggg cgcgccagaa
     5581 ctgcccaaca tcctgacctt tgtttctcac ctgttcggcc cacaggcagc gcatttcatc
     5641 cacgcttacc agaacatcat tttcgcctgg ctcgtaatga ttcttttagg cgtggtggcc
     5701 tacctcgctt ctcgcaagag cgctttgatt cccggccccc tgcaaaattt gatggagatg
     5761 gcggtagagg aactttccaa tttcatcatc ggcatcctcg ggccggaggg aaaacgctac
     5821 gtccctttcc tcggcacgat tttcctctac actctggcga tgaactggtt cggcctaatt
     5881 cccggtttat tctcgcctac cggcggaccc aacggggtga acatcaccgc ggcgcaggcg
     5941 atctgcgtct tcctctacgt ccagtacacc gcgctcacgc ggctggggtt cttcaaatac
     6001 ctgcaccacc tggcggggga accgaaagat gccatcggct ggtccttagt gccgatcaat
     6061 ctgccggtgc acctgatcgg ggagctggcc aagccgttca gtctggcgat ccgtctcttc
     6121 gggaacatta ccggcgagga catcttgatc gcggctttcg tcggcttagg catcgcgctc
     6181 gtagcggtac tcaaatctcc ggtcggcatc ccgctgcagg tgccgttcat tttcttagcg
     6241 cttttgacca gcaccattca ggcgttagtt ttcactttgc tttcgacggt ctacattctg
     6301 ctcgtattgc cgcaccacga agaaggggaa aagcgtgaac attaaccacc agatcacaca
     6361 ggaggagaaa caatggactt ccacacagga ctggcgttag gcgtaccgat cggtttaggg
     6421 ttagccgcta tcggctcggg gatcggcttg ggcggcgccg tgcgcggggc gatggaggcg
     6481 atgggccggc agcccgaagc ttcgggcaag attcaaaccg gcatgatcat cggcgcggcg
     6541 ctctgcgagg cgttgacgat ttactccttg atcgccttct tcatgctgca ggggaggatc
     6601 aagtaaaaag gtggtaggtg gaaggtggta ggtggtgggg aaatatcatc cgccgccaat
     6661 gcacttcctg gtgaacgacg atgaaccttg aactgcagca gatcttaacc cagatcgtcg
     6721 gctttctcat cgcgctctgg attctcaagc gctatgcctg gaaaccgctt ttgaacctgc
     6781 tcgagcaacg gcgggccaaa atcagatccg agttcgacgc catcgaagaa cagaaaaaga
     6841 aaatcgatgc cattgccgcc gattaccggg agaaattaaa agagatcgac gcgctagcac
     6901 gagtacggct gcaggaggcg gtcgccgaag gccaaaaagt cgcggtggaa attcgcgaga
     6961 acgcgcggcg ggaagcgaaa gaacttttgg acaaggccaa agccgaattg gagcgcgagg
     7021 tagccaaagc caaggtgcaa ctgaaaaacg acctggtgga aatgactttg gccgccagcc
     7081 ggaaaatggt caaggagagt ctggataccg ccaagcaccg gcagttgatt tccgatttca
     7141 tttccgatct ggagcgggta acctgatggc gcgcaccaaa cgcaccgggg acgcggcctt
     7201 ggccgaacga tacgccgccg cactctttgc actggcacaa gagaaaggtg ccgtcgaacc
     7261 ggtcgcggct gacgtcgccg gtttggttga gctcttcgac cgcgattcga ctttggaaga
     7321 ttttctcttg gcgccccacg tcttagacga acagaaagag gaattggcgc ggacggcgct
     7381 ttccggaaag gtgttgccca cctttctcga tttccttctg ctccttctgg ataggcaccg
     7441 cttcgaccaa ttccgggcca tcgcccgccg ttaccaggag ctgatggaag aggatcaggg
     7501 catcctgcga gcgcaggtgg tgaccgtggt cgatcttccg ccggtggaag ccctccggct
     7561 gaaagccaaa ctcgaacaga agacaaaaaa gaaaattctg ctcaaaccgg tcgttgatcc
     7621 cgaaatcttg ggcgggatga tcgtcaaact caaacaccag atcatcgacc ggagtattgc
     7681 gcagcaattg gttgaactca aggagcagct tctggcagtc aaagtgcatt agaaaatcag
     7741 cattaagaga acaatcaata ggtgggtagg tcgggaatct tgcctgaccg cgagcactaa
     7801 acggagacca gtaaatggcg atacggccgg aagaagtcag cgcgat
//
LOCUS       DBNGZD010000118         8782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_644206, whole genome shotgun sequence.
ACCESSION   DBNGZD010000118 DBNGZD010000000
VERSION     DBNGZD010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_644206"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="bzdQ"
                     /locus_tag="AC16GM_03205"
     CDS             <1..871
                     /gene="bzdQ"
                     /locus_tag="AC16GM_03205"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA reductase, bzd-type, subunit Q"
                     /protein_id="HIR2922716.1"
                     /translation="DAQFWRWKEYNWKAPDADWREAKSVTAGIDVGSVSSQAVILTDG
                     KIFAYSSQRTGSDSPQSAQKAMDWALDGTGLSLENIQFIVGTGYGRVNVPFAKKAITE
                     IACHARGANFMYGSSVRTILDMGGQDCKAIRCDEKGKVTAFLMNDKCAAGTGRGMEVF
                     ADLLSVPIEEIGDLSFTVDAEPPPVSCTCVIFAKSEALRLLRRGWAKNKVVAAYCSAM
                     AHRVVTLLERIGVEQAFAITGGIGKNRGVVQRIEQELHVKALAPTWDTQIAGALGAAL
                     FAHALLEKSRSSN"
     gene            882..1163
                     /locus_tag="AC16GM_03210"
     CDS             882..1163
                     /locus_tag="AC16GM_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2922717.1"
                     /translation="MLANYGYKDGSGEYFITIDTDRCAACTDRGCIQACPVQVLEIII
                     DDYEQEVASVAAEHRKKLKFSCASCKPNVGRSELPCMAACEPKAISHSW"
     gene            1177..3711
                     /locus_tag="AC16GM_03215"
     CDS             1177..3711
                     /locus_tag="AC16GM_03215"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR2922718.1"
                     /translation="METISLTIDGEQVRIAAGSSILSACQKANIYVPTLCSHPDLSPP
                     FDLAPSDFIFRGNQKIENADPSSRRYPGCRLCLVQVQGEDRPVRACATEALNGQIIST
                     NTDKVNLKRQENLSSILANHPHACLTCAQNRGCSLEPCTNNVAVVERCCPKFNRCEIQ
                     KVAEFIGISRETPRYTFRNLPVVKGEPLYAIDYNLCIGCLRCVQICREQRGVGALGFV
                     FQNGEVLVGTIAPTLKDSGCKFCGACVEVCPTGTLLDKAIKGIPTERDLVPCISACPV
                     GMDVPGYVTAIAERDLSRARKIIKEKAPFPRVLSRVCFHPCEQECRRSFLNEPVAICA
                     LKRFAFESGSEEEEEEKVSRTTGRKVAVVGAGPAGLTAAYYLARSGHSVAVFESGAEP
                     GGMLRYGIPDFRLPRALVAKEISQILKQGIELRTNTVFGRDITLETLQPNFDALFLAF
                     GAQGSKKLKLEGIDREGVLWGLDFLRKVKSGERIRLKDKVAVIGGGDVAIDVAMTALR
                     LNAQEVHLFCLEERDRMPAHPWQIEQALDEGVVIHCSWGPKRIVGDGNQVAGVELVKC
                     LSVFEQDGTFQPAFDESQGIFQEAGSIIFAIGQTLDASIFVDGISRRLVQDGKIRIKP
                     DTLETSLSGVFAGGDAVSGPASVVEAIQMGRRAAVAIDKFLGGEGRLEGKAGEEKRTA
                     SRDHPGWLGREDGFAGYSRIPIPTPPAEQRKKSFAELECGYDLEHGVKEAQRCLRCHL
                     RLQISRIGLPPEKHLEFTEGKIKTIPDIEGVFQLLDTNRKVILISGTSHLRESLHGEL
                     KTRKQAAYFIFEENKMYTQRETELLQQFLQRYGKLPEGNDEVGDLY"
     gene            complement(3724..4491)
                     /locus_tag="AC16GM_03220"
     CDS             complement(3724..4491)
                     /locus_tag="AC16GM_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR2922719.1"
                     /translation="MNFKNIFIETREGVAELTLNRPPLNILNIAMLQELNAILGGLNA
                     STELKLLVIKATGKSFSAGVDVGEHTKDKVTEMIKTFHRMFLLLDDLEIPTLAVVQGP
                     ALGGGCELATFCDLVIASEEAKFGQPEIKVGVFPPVAIAYFPTVVPGKRGLELLLTGE
                     PVTAPEALALGLVNRVYPQAMLEAEVKKFIEKIKSLSAVVLRLTKRNWKEARKTNFQQ
                     TLKKAEKVYLGDLMSTLDANEGLSAFLEKRAPVWKNR"
     gene            complement(4501..5238)
                     /locus_tag="AC16GM_03225"
     CDS             complement(4501..5238)
                     /locus_tag="AC16GM_03225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2922720.1"
                     /translation="MELQQKRALVTGGSAGIGRAIVLDLASNGADVAFAYLGPEVAEV
                     KNRVERLGRKCLPIYTDVSIFAEAQKTVSLVIKTFGGIDILVNNAGINWDGVIWKMTE
                     EMWDRVLAVDLKGCFNYTRAVAPIFKEQKYGKIVNIASINALRGKFGQANYAAAKAGV
                     IGLTKSVAVEMGKYNVNVNAVAPGFIETGMTEKLPEDIKKQAYNETVLKRFGSPEDVA
                     HLVTFLCSEKARHITGAVIQIDGGQNI"
     gene            complement(5284..6240)
                     /locus_tag="AC16GM_03230"
     CDS             complement(5284..6240)
                     /locus_tag="AC16GM_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR2922721.1"
                     /translation="MHAFQPASAEKFKFQEIIYEKKDWVARVTINRPESYNAYSTQAL
                     KELIAAFQDASWDDAIGVVVFTGAGDKAFCTGGDVKEYASVYTKKPRDYWKYMGLFRA
                     YLESILNLGKPTIARINGMAVGGGNESQMACDLAIMAEHAYLGQIGTNVGSVACGGAT
                     QWLPILIGDRRARYMLLLNERIPAKQALEWGLVNQVVPYEKLDAAVSDMCQKLLAKFP
                     ECTRYTKAQVNFWKELSWHNTIGHARDWLALHFTSLEPHEGMQAFVEKRKVDYMRLRK
                     LAAEGKSSEFLWGPYSLDCKKCGAKGIPEEFMFCGKCGNKLQ"
     gene            6778..7344
                     /gene="liaF"
                     /locus_tag="AC16GM_03235"
     CDS             6778..7344
                     /gene="liaF"
                     /locus_tag="AC16GM_03235"
                     /inference="COORDINATES: protein motif:HMM:NF021443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HIR2922722.1"
                     /translation="MAQSKGGTFIGLLLVLIGFLFLLNSLDLVDFGRSLGILWPLLLI
                     GVGIWFMLRHRRRPGTTVNPEDHRSTMQLFGDVGYTANGRVPERLTVSILFGDAQIDL
                     TRGEFPYPTHYIDHSSVFGDLKIRVPPSLAVSVSGHTLFGDTDVFGKKSGGFAQNLEL
                     AGGAAAGDARRLVISTNVVFGDVEVRTP"
     gene            7341..7607
                     /locus_tag="AC16GM_03240"
     CDS             7341..7607
                     /locus_tag="AC16GM_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922723.1"
                     /translation="MRLMTTPYDRVAAVAPELSRKASQVAEEILAFFFEPEELTDEKY
                     ENYAFVIENIVAEYLESSASRYLAARFTREMEQNRTDEPKAKDN"
     gene            7582..>8782
                     /locus_tag="AC16GM_03245"
     CDS             7582..>8782
                     /locus_tag="AC16GM_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HIR2922724.1"
                     /translation="MNQKQRTIKSPVSFSGVGLHTGNQATIAFKPAPANTGIVFVRTD
                     LPGRPKVPADIDWVSDITRGTTLAKGDVKIYTVEHVLAAAAGLEIDNLSVEMDNNEPP
                     VGDGSALPYVEKLLQAGIVEQDEPKVFLEIDQPLAYSEPSRGVDLVVLPSDDFRITFM
                     IDYRNPALGTQYTTLASLEQEFVKEFAPARTFCFLHEVEELKKLGLIKGGSLDSAIVI
                     CEDGVAASDLKRLKKLFGDGRDLSVGKNGILNDTTLRFPNEPVRHKAIDLLGDLSLIG
                     MPLKAHVLAARSGHAANVALARKIRGLYQKEKIIKKYQAQKAAKDSVLDINTILRLLP
                     HRYPFLLIARFIDLEPEQRVVALNNVNINEPFFAGHFPGHPIMPGVLIIEAMAQAGGF
                     LLLQTVEE"
BASE COUNT         2066 a         2321 c         2360 g         2035 t
ORIGIN      
        1 ggacgcacaa ttctggagat ggaaagaata taactggaag gcgccggacg ccgactggcg
       61 agaggcgaag agcgtcaccg ccggcatcga tgtcggctcg gtcagttcac aggcggtcat
      121 tttgaccgac gggaaaattt ttgcgtactc cagccagaga accggctccg acagcccgca
      181 gagtgctcag aaagctatgg actgggcgct ggacggaacc gggttaagtc tcgaaaatat
      241 ccagttcatc gtgggcaccg gctacggcag agtcaatgtt ccttttgcga aaaaagccat
      301 aaccgaaatt gcctgccacg cgcggggtgc caatttcatg tacggctctt cggtgcgcac
      361 gattctggac atgggcgggc aggactgcaa agccatccgc tgtgatgaaa aaggcaaagt
      421 gaccgccttc ttgatgaacg acaaatgcgc ggcgggcacc ggccgaggga tggaggtctt
      481 tgccgattta ctgtctgttc ccattgagga gatcggagat ctctccttta cggtcgacgc
      541 ggaacctccc ccggtcagct gcacctgcgt cattttcgcc aaatccgagg ccttgcggtt
      601 gctccggcgg ggctgggcca agaacaaagt cgtggcggcc tactgttcgg cgatggccca
      661 ccgggtcgtg acgctcttgg agagaatcgg ggtggaacaa gcgttcgcca ttaccggcgg
      721 catcgggaaa aatcgcgggg tcgtccagcg cattgaacag gagttacacg taaaagcgct
      781 ggcacccacc tgggataccc agattgccgg ggcattagga gcggcgctgt ttgctcacgc
      841 tcttttggag aagtcccgat cgtccaactg agggtaaaac catgttggcg aattacgggt
      901 acaaagacgg ttcgggcgaa tatttcatca ccatcgatac cgaccggtgt gctgcctgca
      961 ccgaccgggg gtgcattcaa gcttgtccgg tgcaagtttt ggagatcata atagatgact
     1021 atgagcaaga agtcgcttct gttgctgctg aacaccgcaa aaaattaaaa tttagctgtg
     1081 cctcgtgtaa accgaatgtg ggaaggagcg agctgccttg tatggccgcc tgcgagccaa
     1141 aagccatcag ccactcctgg tgagaagtcg gttcttatgg aaaccatttc tctgaccatt
     1201 gatggggaac aagtaagaat tgccgccggc tcaagtatcc tctctgcctg ccagaaagcc
     1261 aacatctatg ttcccactct ctgctctcat cctgatttgt ccccaccttt tgacctggct
     1321 ccctcggatt tcatcttccg gggtaaccag aaaatagaga atgctgaccc cagttcccgg
     1381 aggtacccgg ggtgtaggct ctgtctggtc caggttcaag gcgaggaccg gccagtacgg
     1441 gcttgtgcga cagaagcgtt gaacgggcag ataatctcca ccaataccga taaggtaaac
     1501 ctgaagcgtc aggagaattt gtcttcaatc ttagccaacc atccgcatgc atgtttgacc
     1561 tgtgcccaaa accggggctg ctcgctggaa ccctgcacca ataacgtggc agtcgttgag
     1621 cgttgttgtc ccaagttcaa ccggtgcgaa attcaaaagg tggcggaatt catcggcatc
     1681 agcagagaaa caccccggta caccttccgg aatctgccgg tcgtcaaagg cgagccgctc
     1741 tatgctattg attacaatct ctgcatcggt tgcctgcgct gtgtgcagat ttgcagagaa
     1801 caaagaggtg taggggcctt gggttttgtg tttcaaaatg gcgaggtact ggtgggaacg
     1861 attgctccca ccctcaaaga ttccggctgc aaattctgtg gcgcctgcgt ggaagtctgt
     1921 cctaccggca cccttttgga taaggcgata aaaggtatcc ccactgaaag ggatttagtc
     1981 ccctgcattt ccgcctgccc ggtgggaatg gacgttccgg ggtacgtcac tgcgattgcc
     2041 gaaagggact tgagccgggc ccggaaaatc ataaaagaga aggctccttt tccccgagtc
     2101 ttaagccggg tctgttttca cccctgtgaa caggaatgtc ggcgttcgtt tctcaatgaa
     2161 ccggtggcta tctgcgcgtt aaaaagattc gctttcgagt ctggatcaga agaagaagag
     2221 gaagaaaaag tatcccgcac cacgggtcgg aaagttgccg tcgtcggcgc cggccccgcg
     2281 gggctgactg cagcctacta tttagcgcga tcgggacatt cggttgcggt ctttgaatct
     2341 ggagcagaac ccggagggat gctgcggtac ggtatcccag acttccggtt acccagggct
     2401 ctcgtagcga aagagataag tcaaattctc aagcagggca ttgaactgag aacgaatacc
     2461 gtctttggaa gagacatcac gttagagacc ttgcagccca atttcgacgc cctcttttta
     2521 gctttcggtg ctcaagggtc gaagaaactg aagttagaag gcatcgaccg ggagggagtg
     2581 ctgtggggtt tggatttttt aagaaaggta aagtcggggg aaagaataag actgaaagat
     2641 aaagtcgcgg ttattggggg aggagatgtg gccattgatg tggccatgac ggccttaaga
     2701 cttaatgccc aagaggttca ccttttctgc ctggaagaaa gagaccggat gccggcccac
     2761 ccgtggcaga tagagcaagc gctcgatgaa ggggtggtaa tacactgttc gtgggggccc
     2821 aagaggattg tcggagacgg gaatcaggtt gccggggtgg agctggtgaa gtgcctttct
     2881 gtttttgaac aggacgggac tttccagccc gcctttgacg aatctcaagg cattttccag
     2941 gaagctggtt cgataatttt tgcgatcggc cagacgctgg atgcctcgat ttttgtggac
     3001 ggtatctcca ggaggttagt tcaagacggc aaaataagaa tcaagccaga tactctggag
     3061 acgagccttt ccggcgtttt tgccggaggg gacgccgttt ccgggccggc ctcggtcgtc
     3121 gaggccatcc agatgggcag gagggccgca gttgcgattg acaaattttt aggcggagaa
     3181 ggtcgtttgg agggaaaagc cggggaggag aagagaacgg cctctcgcga ccaccccgga
     3241 tggctgggaa gagaggacgg ttttgccgga tactctcgaa ttccgattcc cactccgcct
     3301 gcggaacaga ggaagaaaag ttttgccgaa ttagaatgcg gttatgatct ggagcacggg
     3361 gtaaaagaag cccagagatg tttgcgctgt cacctgaggc tgcaaatctc ccgaatcgga
     3421 ctgccaccag aaaagcactt agaatttact gaaggaaaga tcaagaccat acccgatatc
     3481 gaaggagttt tccaactgct tgacacgaac cgaaaggtta ttctcatttc cggcacgtcc
     3541 catctgcggg aaagcctaca cggagaactc aagacccgaa aacaggcggc ctattttatc
     3601 tttgaagaaa acaaaatgta tacccagaga gagaccgagc ttttgcagca attcttgcaa
     3661 cggtacggga aactgccgga aggaaacgat gaggtaggcg acctttacta acaggtagtc
     3721 tcctcaccta ttcttccaga ccggggctct tttctctaag aaggcagaca atccttcatt
     3781 cgcatccagg gtggacatca agtctcccaa ataaaccttt tccgctttct ttaaggtttg
     3841 ttgaaaattc gtcttccggg cttctttcca gtttcttttc gtcagccgga gcacaacggc
     3901 actcaaactt ttgatttttt caataaattt tttcacttca gcttccagca tagcctgtgg
     3961 gtacactcgg ttcacgaggc ccagggccaa tgcttccggg gcggtcacgg gttcgccggt
     4021 caaaagaagt tccaaccctc ttttccccgg tacgacggtg gggaaatagg caatcgctac
     4081 gggaggaaaa acaccgactt taatctccgg ctggccaaat ttcgcctcct cggaggcaat
     4141 gaccagatcg cagaaagtcg ccaactcaca gcccccgcct aaggcaggtc cctgaacgac
     4201 tgcgagcgtc ggaatttcta agtcgtctaa gagcaaaaac atccggtgaa aagtttttat
     4261 catttcagtt actttatcct tggtatgttc gcccacatcc acgccggcag aaaaagattt
     4321 gccagtggct tttattacta aaagtttcaa ctcggtggaa gcgttgagtc cacccagaat
     4381 agcattaagc tcctgcagca tcgctatgtt gagaatattc agaggaggac ggtttagcgt
     4441 gagttcggcc acgccctctc tagtttcaat gaagatgttc ttgaaattca ggctcgcctc
     4501 ctaaatgttc tgcccaccgt caatttgtat caccgcaccg gtaatatgcc gggctttctc
     4561 agaacagaga aacgtgacga gatgagccac atcctcgggg ctgccgaatc tcttcagcac
     4621 ggtttcatta tacgcttgtt tttttatatc ttccgggagt ttttcggtca tgccggtttc
     4681 aatgaagccc ggcgccaccg cattcacatt gacgttgtat ttccccattt caacggcgac
     4741 tgatttcgtc aggccaatga ccccggcctt cgccgccgca tagttcgcct gtccaaattt
     4801 cccccgcagc gcgttaatcg aggcgatgtt cacaattttc ccgtactttt gctctttgaa
     4861 aattggtgcg accgcccggg tgtagttgaa acagcctttc aaatccaccg cgagaactct
     4921 atcccacatt tcttcggtca ttttccatat gaccccgtcc caattgattc ccgcgttatt
     4981 aacgagtatg tcaattccac cgaatgtttt gattaccaac gagacggttt tctgggcttc
     5041 ggcgaaaatg gaaacgtcag tataaatcgg cagacatttt ctgcccaatc gttctacgcg
     5101 attctttacc tccgcgactt ccggacctaa gtacgcaaag gcaacatcag ccccatttga
     5161 agccagatcc aaaacaatcg cccggccgat gcctgcgctc ccgccggtaa ccagagctct
     5221 tttttgctgt aattccatct actttgagcg tctgcttcct gtagacctcg aggcttttat
     5281 tcgctactgc aatttattcc cgcatttccc acagaacata aattcttccg gaatcccttt
     5341 ggccccgcac tttttacaat ccaaagaata gggcccccat aagaattcag aggatttacc
     5401 ttcagcggct aattttctta accgcatata gtcaaccttt ctcttttcca cgaaagcctg
     5461 catcccctca tggggttcta aggaagtgaa gtgcaaggcg agccagtcgc gcgcatgacc
     5521 gatggtgtta tgccaggaga gttctttcca gaagttcacc tgagctttcg tataacgcgt
     5581 acattctgga aatttagcca aaagcttctg gcacatgtcg gagaccgcgg cgtcaagttt
     5641 ttcataaggg accacctgat ttactaaacc ccattctaaa gcctgtttcg ccggtattcg
     5701 ctcatttaat aaaagcatat accgggctct ccggtcgccg atcaaaatcg gcagccattg
     5761 cgtagcaccg ccgcaagcga ccgagccgac attggttccg atttggccca agtacgcgtg
     5821 ctcggccatg atagccagat cgcaggccat ctgggattca ttaccaccac ccacggccat
     5881 accattgatg cgcgcaatcg tcggtttgcc caggttgaga atactttcca aataggcccg
     5941 gaaaagcccc atatatttcc agtagtcgcg cggtttcttc gtgtaaaccg atgcgtactc
     6001 tttcacatct ccgccggtgc aaaacgcttt gtctcctgct ccggtaaaaa ctaccactcc
     6061 aatagcatca tcccaggatg catcctgaaa agccgcgatc aattctttta aagcttgagt
     6121 gctataggcg ttgtatgatt cggggcggtt tatcgtaact cttgccaccc aatctttttt
     6181 ctcatagata atttcttgga atttaaactt ttctgccgaa gctggctgaa atgcatgcat
     6241 tcaaaatcct cgctttccgg acacaaaccg tccttaaaaa tctatctcca taacgattga
     6301 ggtaggaatt tgggagtaaa tggccggctt tctgccgaca gagtccacgc ggcgaattta
     6361 cgggagggga aacgtgaagt caacagagga atcgccgcga cgcggaaccg gtattccgcg
     6421 gttatttgcc cgccttttag ggttgttaat gttcttaggt tcctcagagc atgcagtcgt
     6481 tacagcgagt caattatctt caactggcca ccgaggaagc tgcggcaact ccagaaagat
     6541 atcaaccaaa atgacgttat ggtgaatcag aaaatgctcg cttgagccga cttgggccgc
     6601 aacctgccgt tgcaaaagtc cccggtctaa ccttatcttt caaatgtgat ctcccaagct
     6661 gttaaaggac ttagggtaag tcccggcagg tgtaggagag gtgcagtgcc gttcgcagga
     6721 ttgatactgt ggttgtgaaa agtcaggtgg taggtaaaac aattaaggga gaaactggtg
     6781 gcacagagta aagggggaac gttcatcggc ttgcttttgg ttctcattgg cttccttttt
     6841 ctcctgaaca gcctcgacct ggttgacttt ggccgctcgc tcggcatcct ctggccgctt
     6901 ttgctcatcg gcgtagggat ctggtttatg ctccgtcacc gacggcgacc gggtacgacg
     6961 gtcaatccgg aagatcaccg ctcaaccatg caactttttg gcgacgtggg gtacaccgcc
     7021 aacggaaggg taccggagcg cttgacggtt tccatcctct tcggagatgc ccaaatagac
     7081 ctcacccggg gtgaatttcc ttacccgacg cattatattg atcacagttc ggtcttcggc
     7141 gatctgaaaa ttagggtgcc gccgtcgctt gcggtctcgg tttccggcca cacgctcttc
     7201 ggcgacacag acgtcttcgg aaagaagtcg ggaggattcg cccagaacct cgaactggcc
     7261 ggaggagctg cagccggtga cgccagaagg cttgtgattt caaccaacgt cgttttcggc
     7321 gacgtggagg tgcggacgcc atgagactaa tgacaacccc ctacgaccgg gtagcagccg
     7381 tagcaccgga attgagccgg aaagcctcac aggtggcgga ggagattctc gcgttcttct
     7441 tcgagccgga agaattgacc gacgaaaagt acgagaacta tgcgttcgtg atcgagaaca
     7501 tcgtggcgga gtacttagaa tccagcgcca gccggtactt agccgcccga ttcacccggg
     7561 aaatggaaca gaaccgcacg gatgaaccaa aagcaaagga caattaaatc tccggtatct
     7621 ttctccgggg tcgggctgca caccggcaat caggcgacga tcgccttcaa gccggctccg
     7681 gcgaacaccg gcattgtctt cgtccgcacc gacctgccgg ggaggccaaa ggtacccgcg
     7741 gatatcgatt gggtctcgga catcacccgc ggcaccacgc tcgccaaagg cgacgtgaaa
     7801 atctacaccg tggagcacgt tctggccgcg gcggccgggc tggaaatcga taatctctcc
     7861 gtcgagatgg acaacaacga gccgccggtg ggggatgggt cggcgttgcc ctacgtggag
     7921 aaactgctgc aggccggcat cgtcgaacag gacgagccga aggtttttct ggagatcgac
     7981 cagccgctgg cctactccga gccctcccgg ggcgtcgatc tggtcgtttt gccctcggac
     8041 gacttccgga tcaccttcat gatcgactac cgcaatccgg ccctgggcac ccagtacacc
     8101 acgttagcat cgctggagca agaattcgtc aaagagttcg cccccgcgcg gactttctgt
     8161 tttctgcacg aggtggagga actgaagaag ctggggctga tcaaaggcgg gagtttggat
     8221 tccgccatcg tcatttgcga ggacggggtg gcagcgtccg atttgaagcg gttgaagaag
     8281 ttattcgggg acgggcggga cttgagcgtc ggcaagaacg gcatcctgaa cgatacgacg
     8341 ttacgctttc ccaacgagcc ggtgcggcat aaggccatcg accttctggg cgacctctcc
     8401 ctgatcggca tgccgctcaa agcgcacgtt ctggcggccc gttccggcca cgcggccaac
     8461 gtggctttgg cccgaaaaat ccgcgggctg taccagaagg aaaaaattat taagaaatat
     8521 caagcgcaga aagccgccaa agattcagtc ttagacatca acacaattct gcggttgctg
     8581 ccgcaccgct acccgttcct cttgattgcc cggttcattg acctggaacc ggagcagcgg
     8641 gtggtggcgc tcaacaacgt caacatcaac gagccgttct tcgccgggca ctttccgggt
     8701 cacccgatca tgccgggggt gctcatcatc gaggcgatgg cgcaagccgg tgggttcctc
     8761 ctcctccaga ccgtcgagga gc
//
LOCUS       DBNGZD010000119         3388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1339834, whole genome shotgun sequence.
ACCESSION   DBNGZD010000119 DBNGZD010000000
VERSION     DBNGZD010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1339834"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..755)
                     /locus_tag="AC16GM_03250"
     CDS             complement(66..755)
                     /locus_tag="AC16GM_03250"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR2922725.1"
                     /translation="MDKTGKMVIKPQFYSAYDFSEGLAMVFTGHWGYIDKTGKMVIEP
                     QFDDAWKFSEGLAPVQKIYGKEWGFIDRTGKMIIPSQFDKAESFSEGLALVKINWKRG
                     YIDKTGKTLIPPQFDTAMSFSEGLAAVRIDGKWGFIDKLGRYVVKPKFHDADNFSEGL
                     ARVQINSKWAFIDKTGKEIIKPKFDFAWNFSEELAFVEIDKKWGCIDKTGRVKSHSSH
                     QIDMLVAWQDD"
     gene            complement(894..1544)
                     /locus_tag="AC16GM_03255"
     CDS             complement(894..1544)
                     /locus_tag="AC16GM_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922726.1"
                     /translation="MNLERLSTVGYLFIGLGVAILLWGLFRKLKDSTRKPPARQDESS
                     GPSIVGPILLAALLVGLGQLLVWLVGEHENFYPPKPDRPVASLKLVIPKSGDPYLEVQ
                     ELYKSGRYRLLLSKGPGELCRITANAIKWRKEFSFLGLKEYLRLTEVVVRDTADSAGA
                     RPAKLVLSEEAQEFASFLRSSNLPVVNSKEIASAPFPLLPDHEYQVFLQNDKFEIE"
     gene            complement(1680..2489)
                     /locus_tag="AC16GM_03260"
     CDS             complement(1680..2489)
                     /locus_tag="AC16GM_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR2922727.1"
                     /translation="MRKIRHMTFTVFSEDDQKALSIRLPSIIFYLIIGLLIATVIGLV
                     ISVLYSSRLAAKTSLLESVQIENQKLRNYTSKITLLEKELFDNRVLTVKLAKMAGIEL
                     PMLQDSSKSPTNVSMPESSKVVVSETISSSVASVLPPQKEALPTGLPASGTRLPRSLL
                     SGSDLLLPGTFLAATARVSVRATAEGTVEFSGRDSSLGNLVVVSHAQGVKTRYGRLGE
                     LKVSRGQKVNKGQTLGYTTPPDSTGKSIFYYEVQGTTNPPKRSDLSASSDS"
     gene            complement(2542..>3388)
                     /locus_tag="AC16GM_03265"
     CDS             complement(2542..>3388)
                     /locus_tag="AC16GM_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR2922728.1"
                     /translation="QKIALGKGLSALIPEGETGMEGESIQPILLAKIQPNPDQPRQAF
                     DPERLAELANSLKDQGVLQPIIVRPVGENYQVVAGERRVRAAQLAGFESIPALVRENV
                     SKEMALELALIENLQREDLNPIEEARAYKQLATEFGLSQEAIAGKVGKERSSVANALR
                     LLNLPQGIQTKIAEGELSPGQARLLLRLSDPQRQNQLAERIVQGSISVRELERLIESP
                     RPIRRRLKLKRPAALFDTIEQALKRFFGTNVILRRGKKRGKIVIEFYSDDDLNRILQL
                     LKIAV"
BASE COUNT          774 a          874 c          852 g          888 t
ORIGIN      
        1 gtgtgcacac cggcgttttc tccctcggag aatccagtac ttgccactgt cgcagcgtgg
       61 acacctcagt cgtcttgcca tgccaccaac atatcaattt ggtggctgga atgggacttt
      121 acccttcctg ttttgtcgat gcaaccccat tttttgtcaa tttccacaaa agcaagctct
      181 tcagaaaaat tccaagcgaa atcgaacttt ggtttaatta tttcctttcc tgttttgtca
      241 ataaaagccc acttactatt aatttgcaca cgtgccaacc cttcagaaaa attgtcagca
      301 tcatgaaact tgggttttac aacatatctc cccagtttgt cgataaatcc ccatttccca
      361 tctattcgta cagctgctaa accttcagag aaactcatag cggtgtcgaa ttgcggaggg
      421 atgagcgttt ttccggtctt gtctatataa ccgcgtttcc aattgatctt tacaagtgct
      481 aatccttcgg aaaaagattc agctttgtcg aactgcgagg ggatgatcat ttttccagtc
      541 ctatcgataa agccccattc ttttccgtat attttctgaa ctggtgcaag gccttcagaa
      601 aatttccaag catcatcaaa ttgtggttca ataaccattt taccagtctt gtcgatatat
      661 ccccaatggc ccgtaaatac cattgctaaa ccttcagaaa aatcgtaggc actataaaat
      721 tggggtttga taaccatttt gccggtcttg tccatgtacc catatctgcc actacacgac
      781 caaaccaaag tcgcaatgct caccaagagt aatgtacttt tcctcattac catttcagtg
      841 aagctccttt gaccatcttt acccttttca agttggtatg gacgactcaa tatctattct
      901 atctcaaatt tatcattctg caggaacacc tggtactcgt ggtcggggag gagaggaaaa
      961 ggagcgctgg cgatctcttt ggagttcacc accggcaagt tcgaggaacg caggaaagag
     1021 gcgaattcct gggcctcttc gctcaatact aatttcgccg gtcgcgctcc ggctgaatcg
     1081 gcggtgtccc gtactaccac ttcggttaag cgcaggtatt ctttgagccc cagaaaggag
     1141 aattcctttc tccatttgat ggcgttggcg gttatgcggc agagctcgcc ggggcctttg
     1201 gaaagaagca ggcggtaccg gcccgatttg tacagctcct gaacctccag gtaggggtct
     1261 cccgatttgg ggattaccaa tttgaggctg gctaccggac ggtcgggctt gggcggatag
     1321 aagttttcgt gctcccccac tagccagacc aaaagttggc ccagtccgac taagagtgcg
     1381 gccaggagaa tgggcccgac gatgctcggc ccggatgact catcctggcg ggcggggggc
     1441 ttgcgagtcg aatccttcaa tttgcggaaa agcccccaga ggagtatcgc tacgcccagt
     1501 ccgataaaga gatatccaac cgtcgaaagc cgctcgagat tcataatttt tcacacgccg
     1561 aaagaacgca aaaaccgatc atttccgaca tgaaattgac ctgtagccga ggtgtcgcta
     1621 cgactctacc gtcaaacttc ggctgcgact ttgcacgggc cggtagaggc ccgctatatc
     1681 taactatcgg aagaagccga gaggtctgaa cgtttgggtg ggttggtggt cccctgcact
     1741 tcgtagtaaa agatcgattt tccggtcgag tccggcggtg tggtgtagcc gagcgtctgg
     1801 cctttattca ctttttgccc ccggctgacc ttcaattctc caaggcggcc gtagcgggtt
     1861 ttcactccct gtgcatggga aacgaccacc agattgccca ggctggagtc tctgccggaa
     1921 aactcgactg tcccttccgc cgtcgctcga acggaaactc gggcggtcgc ggccagaaac
     1981 gtcccgggca gaagcaggtc agatccagag agaagagagc gggggaggcg ggtgccactg
     2041 gccggcaagc cggttggcag tgcttctttt tgaggaggca aaactgacgc taccgatgaa
     2101 gaaatagttt cacttacaac cacttttgat gattcaggca tcgaaacatt ggtaggtgat
     2161 ttgctcgaat cttgtaacat tggtaattct attcctgcca ttttggcaag ttttactgtc
     2221 aaaacgcgat tatcaaacag ttctttttcc aacaaagtga ttttggatgt gtagtttctt
     2281 aatttttgat tttcaatttg taccgattcg agaagtgaag tcttggcagc caggcggctg
     2341 gaatagagaa ctgaaatgac caatccgatc acggtggcaa tgagcaatcc aatgatcaaa
     2401 taaaaaatga tgctgggaag gcggatggaa agtgcctttt ggtcgtcttc cgagaaaacc
     2461 gtaaaggtca tatggcgaat tttccgcatc aggacctcct ttccttatca aatcggctgt
     2521 tgggccggca tcctttgccg ctcagacggc gattttgagg agttgaagaa tccggttcag
     2581 gtcgtcgtcc gaatagaatt cgatgacgat tttgccgcgc tttttgcccc ggcgcaggat
     2641 cacgttcgtt ccgaagaagc gcttgagagc ctgttcaatc gtgtcgaaga gcgccgcggg
     2701 acgtttgagt ttcagccggc gccggatggg tcggggtgat tcaatcaagc gttccaattc
     2761 ccgtacggag attgatccct gcacgattcg ctctgcgagt tgattttgcc gctgggggtc
     2821 cgaaagccgc aagaggagcc gcgcctgtcc cggagagagc tcgccctccg cgatttttgt
     2881 ctgaattcct tgtggcagat tcagaagccg gagcgcattc gccacggagg aacgttcttt
     2941 acccactttg ccagctatgg cttcctgcga cagaccgaat tcggtcgcca gttgcttata
     3001 ggcccgggct tcctcaatgg ggtttaaatc ttcccgctgg aggttttcaa tcaacgccaa
     3061 ttccagcgcc atttctttcg acacattttc ccgcaccagt gccggaatag attcaaatcc
     3121 ggcaagctgt gcggcccgca cccggcgttc gccggcgact acctggtagt tttcgcctac
     3181 cggccggacg ataatcggtt gaagcactcc ctgatctttc agggaattgg ccagttcggc
     3241 caggcgttcc ggatcaaagg cctggcgcgg ctggtcgggg ttcggttgaa ttttcgccag
     3301 aagaattggc tggatacttt ctccttccat tcccgtttcg ccttccggaa tcagggcgct
     3361 caggcccttg cccaaagcga tcttttgc
//
LOCUS       DBNGZD010000120         5972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_993433, whole genome shotgun sequence.
ACCESSION   DBNGZD010000120 DBNGZD010000000
VERSION     DBNGZD010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_993433"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /gene="topA"
                     /locus_tag="AC16GM_03270"
     CDS             complement(<1..1720)
                     /gene="topA"
                     /locus_tag="AC16GM_03270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR2922729.1"
                     /translation="MAKNLIIVESPTKSKTLVKFLGRDFSVFATKGHVIDLPKSRLGV
                     DLKRNFKPTYVVVPGRKEVLEALAEKAKGVKKIYLAPDPDREGEAIAWHVANRLAILK
                     KPVLRATFNEITKSAVLRGIEKAGKIDLDLVNAQQARRILDRLVGYQVSPLLWKTVHR
                     GLSAGRVQSVALRMICEREEAVKNFVPQEYWTIAATFATKKNEAFIARLVKIDGANPE
                     IPSQAKADEITADIRKKSYAVSSVTVEEKSRNPFPPYITSTLQQDAARRLFFSAAKTM
                     QIAQQLYEGVELGDEGSVGLITYMRTDSTRTAPEAVEMVRSYISHQFGDKYLPKEPRT
                     FKARKGAQEAHEAIRPTLLDYSPLAIKRYLTKDQFKLYELIWSRFIASQMEAARFEVT
                     TVEITGAPYLFRASAQAVKFDGFLAVYQDLKDENGDEEEAKALPPLKESDPLRLSDVT
                     PSQHFTKPPPRFSEATLVKELEANGIGRPSTYAPTIDTLKKRGYVTTEKRVFTPTELG
                     VTVNRILVEHFPDIFNVEFTAEMEAELDRIEEGEDNWVAVLKDFYGPFSEDVKKADAN
                     HRAIQKT"
     gene            complement(1744..1965)
                     /gene="rpoZ"
                     /locus_tag="AC16GM_03275"
     CDS             complement(1744..1965)
                     /gene="rpoZ"
                     /locus_tag="AC16GM_03275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR2922730.1"
                     /translation="MQKLVPLDDLEKVTRNRYEAVLVAAKRARSLNAQRKQAEEQAGT
                     DSLASDARRVTAQALEELLKGQLKFVRMQ"
     gene            complement(2004..2603)
                     /gene="gmk"
                     /locus_tag="AC16GM_03280"
     CDS             complement(2004..2603)
                     /gene="gmk"
                     /locus_tag="AC16GM_03280"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR2922731.1"
                     /translation="MKRSPRLFIVLSSPSGGGKTTICRKLLARHRDFGFSVSATTRSK
                     RPGERRGRDYHFMSAEEFERKRKQGAFVEWARVYGACYGTLRREVANLARRFPVVLFD
                     VDTRGGLAIKRQYPHTALVFLLPPSSAAQRRRLARRGTDDRATQKARLAASQRELARM
                     TRYDYVVTNERLGQTIRAIESIITAERSKSTCTGTTRTR"
     gene            complement(2748..3614)
                     /locus_tag="AC16GM_03285"
     CDS             complement(2748..3614)
                     /locus_tag="AC16GM_03285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR2922732.1"
                     /translation="MTGFGKGESASGGIRVAVELSSVNNRFCEFQFRLPKFLSAWEPK
                     LKEKLSQALERGKINYALTWEDERGAATYAELNEEAADIYHQLLIRLKKRFKISGKIT
                     LSDFTALPDLIKLNREEVPDEKAWRLIWGATEKALAQLVAMRKSEGTLLAGELMGRIQ
                     RLNEVIALIRGLAEGEVTSYRTRLKNRITELLGAPPVDEQRIALEVALLAEKSDVTEE
                     CVRFLAHNQQFLAALEENVAVGRRLNFLLQEMNREANTIGSKSLSAEIVQRVVVLKEE
                     IEKLREQVQNIQ"
     gene            3731..5503
                     /locus_tag="AC16GM_03290"
     CDS             3731..5503
                     /locus_tag="AC16GM_03290"
                     /inference="COORDINATES: protein motif:HMM:NF017633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled cytosolic
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIR2922733.1"
                     /translation="MLWNSFVIAGVVNFLKGEIASAWVKSVTSNPVQKTLRLELTVGK
                     KQGWLLFNYDARLYHLAWRSFPQSASKEADEKPVFSQLIGAPVNDIEQVNFDRVVKVC
                     FKKREDDKSSLDLIFEFTGRNSNAILVESATNQIAEVLRPVEKAQNRYRRLLSGEVYH
                     PPPAVDQLNPLSITAQALHDRWQKYAELPLIEFLTRHLMGCDHWLAQEIVARANLEPA
                     LSLSQASETSFESLAKTIQALIAKITHPSQPVSVLLENSTPVAIFPFTAVAQAGQEQK
                     SFASIIEAVASAYGTLQQSGTAQVRLAQLQKALANKLEKLEHRHTTLMEEIAGAKQAP
                     YHKKLGDLLTIHHANIPKKAASVTLPDDFDPHGAPITIPLDPAKTPAENAKAYFKKAR
                     KGKTGLPLLEKQLTNVENQLAALKQVQEETEQLTASSPPGDEKLLAQIESELARLGLA
                     PKPQKGQLPIRASKPAKPQFKEFITSEGFRVIVGRNNEENDKITLEVAKGDDFWFHAQ
                     QCPGAHVVLKLDGKREPGKRSLLEAAALAAHFSDARHSRKVPVIYTRAKYVRKPRKAK
                     PGLMTVEREKSLMVPPALLPLKAE"
BASE COUNT         1211 a         1804 c         1635 g         1322 t
ORIGIN      
        1 tcgttttctg aatggcgcgg tggttggcgt cggctttctt gacgtcttcg gagaaggggc
       61 cgtagaaatc tttcagcacc gccacccagt tgtcctcgcc ctcttcgatc cggtccagtt
      121 cggcttccat ttcggcggtg aactccacgt tgaaaatgtc cgggaaatgc tcgaccaaaa
      181 tgcggttgac ggtgacgccc agctcggttg gcgtgaagac ccgcttttcg gtggtgacgt
      241 agccgcgctt tttgagcgtg tcaatcgtcg gcgcgtaggt ggagggccgg ccgatgccgt
      301 tcgcctccaa ttctttgacc aacgtcgcct cggagaatct cggcggcggc ttggtgaagt
      361 gctgggaagg cgtgacgtcg gaaaggcgga gcgggtcgct ttctttcaaa gggggaagcg
      421 ccttcgcctc ttcctcatct ccattttcat ctttcaagtc ctgatagacc gccaggaacc
      481 cgtcgaactt gaccgcttgc gcgctggcgc ggaaaagata cggcgctccc gtgatttcga
      541 ccgtggtgac ctcgaagcgc gccgcttcca tctgcgacgc aatgaagcgg ctccagatta
      601 actcgtagag tttgaactga tccttcgtca aataacgctt gatggccaga ggtgaatagt
      661 ccagcaaggt gggccgaatc gcttcgtgcg cttcctgcgc tcccttgcgc gctttgaacg
      721 tgcgcggctc cttgggcaaa tacttgtcgc cgaactggtg gctgatgtag gagcgcacca
      781 tttcaaccgc ttccggcgcg gtgcgggtgg agtcggtgcg catgtaggtg atgagcccga
      841 cggagccttc gtcgcccaat tccactcctt cgtacaactg ctgggcgatc tgcatcgtct
      901 tggccgccga gaagaagagc cgcctggccg catcctgctg gagggtgctc gtgatgtagg
      961 gcgggaatgg gttgcgcgat ttttcttcta cggtgacgga actgaccgca tacgattttt
     1021 tccggatgtc ggcggtgatt tcatcggctt tggcttgcga gggaatctcc gggttggcgc
     1081 cgtcgatttt caccagccgc gcgatgaacg cttcgttttt cttcgtcgcg aacgtcgcgg
     1141 caatggtcca gtattcctgc gggacaaaat tcttgaccgc ctcttcccgc tcgcagatca
     1201 tccgcagggc caccgactgg acgcgccccg cggagagccc ccggtggacg gtcttccaca
     1261 aaagcgggct cacctggtag ccaaccaggc ggtccagaat gcgccgcgcc tgctgcgcgt
     1321 tgaccaggtc taagtcgatt ttgcccgcct tttcgatccc gcgcaagacc gccgacttgg
     1381 tgatctcgtt gaaggtcgcc cgcagcaccg gtttcttcag aatcgcaagc cggttggcca
     1441 cgtgccaggc aattgcttcc ccctcacggt ccgggtcggg cgccaggtaa atctttttga
     1501 cgcctttggc cttttcggcc agagcctcca agacctcttt gcgccccggc acgacgacgt
     1561 aggtcggttt gaagttgcgc ttcaaatcga cgcccaagcg gcttttgggc aggtcgatga
     1621 cgtgcccctt ggtagcaaag acggaaaaat cccggcccag aaatttcacc agggttttcg
     1681 atttggtcgg cgactcgacg atgattaaat ttttcgccac gtactatcct ttatcggcaa
     1741 acgctactgc atccgcacga acttgagctg gcctttgagc agttcctcca aggcctgcgc
     1801 ggtgacgcgc cgggcgtcgg aggccaggct gtcggtcccc gcctgttcct ccgcctgttt
     1861 gcgctgggcg ttcaaactgc gcgcccgctt ggctgccacc agcaccgctt cgtagcggtt
     1921 ccgggttact ttttccagat cgtccaatgg tacgagtttc tgcatacgct cttttctcct
     1981 tactattgag agtttcggga ttgttaccga gtccgcgtcg tcccggtaca agtcgatttc
     2041 gacctctccg ccgtaattat actttcaatc gcgcgaattg tttgaccgag cctttcgttc
     2101 gtcaccacgt aatcgtacct cgtcatccgc gccagttccc tctgcgaggc ggccaaacgc
     2161 gctttttgcg tggcgcgatc atcggtgccg cgccgtgcca gacgccggcg ctgggcggcc
     2221 gaagatggcg gcaaaaggaa gactaatgcc gtgtgcgggt actgcctctt aatcgccagc
     2281 ccgccgcgtg tatcaacgtc aaagagtaca accggaaacc gccgcgccag gttcgctacc
     2341 tcgcgccgca gcgtgccgta gcaagcgccg tagacccgtg cccattccac gaacgccccc
     2401 tgctttcttt tccgctcaaa ttcctccgcc gacatgaagt ggtaatcgcg accccggcgc
     2461 tcgccgggcc gtttggaccg cgtagtcgcc gaaactgaaa aaccgaagtc ccggtgccgc
     2521 gccagaagtt tccggcagat ggtggtcttg ccgccgccgg agggcgacga gaggacgata
     2581 aacaaacgcg gggagcgctt caccctctgc gtgcggctcc agaaatcagc ttgtgctttt
     2641 cagtgaacac tctgaggaga tcgaaacgaa tcggctccgc ccacttctct aatcttaaac
     2701 tcggaagtgt tggagacttt cggttttctc tggctactgg ccactggcta ctgtatattt
     2761 tgtacctgct cgcgcaattt ttcgatttct tctttgagca ctaccacccg ctggacaatc
     2821 tccgccgaaa gtgatttgga accgatggtg ttcgcctcac ggttcatttc ctgcaggagg
     2881 aaattgagcc gccttcccac cgcaacattt tcctccagcg ccgccagaaa ctgctggttg
     2941 tgcgccagaa agcgcacgca ctcttcggtc acgtcgcttt tttcggccaa gagcgccact
     3001 tccaacgcaa tccgctgctc atccaccggc ggggcgccca gaagttcggt gatccgattt
     3061 ttcaaccggg tgcggtagga agtcacctcg ccttccgcca ggccgcggat gagggcgatc
     3121 acctcattca accgctggat gcgtcccatt agttccccgg ccaacagcgt accttcactt
     3181 ttgcgcattg ccaccagttg cgccagcgcc ttttcggtgg caccccagat gagccgccag
     3241 gccttttcgt cgggaacttc ctcgcggttc agtttgatca agtcgggcag ggcggtgaaa
     3301 tccgacaggg tgatttttcc ggaaattttg aagcgctttt tcagccggat gagcaactgg
     3361 tggtaaatgt ccgcggcttc ttcgttcaat tctgcgtagg tagccgcgcc gcgctcgtct
     3421 tcccacgtaa gggcgtagtt gatcttcccc cgctccaggg cctgcgagag tttctctttc
     3481 agtttaggtt cccacgcgga gagaaacttg ggcaggcgga actggaattc gcagaagcgg
     3541 ttgttgaccg acgagagttc caccgccacg cggatgccgc ctgaggcgga ctcgcctttt
     3601 ccgaacccgg tcatgctgga gatcattccc aatctcacat tatagaaagc gattcgccgg
     3661 ttgtcaaccg gcgccagaaa aggttgaccg ctaccacgcc atcgctacat tgacttccgg
     3721 tggtataacc atgctttgga actccttcgt cattgccggc gtcgtcaact ttctgaaagg
     3781 agagattgca agcgcgtggg tcaaaagcgt tacctccaat ccagtgcaaa aaaccctgcg
     3841 cttggaactg acggttggca aaaagcaggg ctggcttctg ttcaactacg acgcccgact
     3901 ctaccacctt gcctggcgat cttttccgca gtcggcttcg aaagaagcgg acgaaaagcc
     3961 ggtattctca caactcattg gtgcccccgt caacgatatc gagcaggtga acttcgaccg
     4021 cgtcgtcaaa gtctgtttca agaaacgaga agatgataag tcatcgctcg atctgatttt
     4081 tgaattcacc gggcggaaca gcaatgccat tttggtggag tccgcgacaa atcaaatcgc
     4141 tgaagtcttg cgcccggtgg agaaagcgca gaaccgctac cgccgactgt tgtcaggcga
     4201 agtgtaccat ccgccgcccg ccgtggacca actcaacccg ctgagcataa ccgctcaggc
     4261 cttgcacgac cgctggcaga agtatgccga actccctctc atagaatttt tgactcgcca
     4321 tcttatgggg tgcgaccact ggctggcgca ggaaatcgtc gcccgcgcga acctggagcc
     4381 ggcgctttcg ctctcgcagg cgagcgaaac ctctttcgaa tccctggcga aaaccattca
     4441 ggcgctgatc gcaaaaatta cgcatccaag tcaacctgtt tccgttcttc tggagaattc
     4501 tacccctgta gctattttcc ctttcactgc ggtcgcgcaa gcggggcaag agcaaaaatc
     4561 tttcgcctcc ataattgaag cggttgcctc cgcgtatggg actctccagc agagcggcac
     4621 tgcccaagtc cgtttggccc agctccagaa agcgctggcg aataaacttg aaaaactgga
     4681 gcaccgccac acgacgctga tggaggagat tgctggagcg aaacaggccc cctaccacaa
     4741 aaaattaggc gaccttctca ccattcacca cgcgaacatt ccgaagaaag ccgcaagcgt
     4801 aacgctccct gacgactttg acccccacgg cgcgccgatt accatcccgc tcgatcccgc
     4861 aaaaactccg gcggagaatg ccaaggccta cttcaaaaaa gcgcgcaaag ggaaaaccgg
     4921 cctgccgctt ttagaaaaac agttgaccaa cgttgaaaac caactggccg ccttgaaaca
     4981 agtgcaggag gaaacagaac agctcaccgc ttcctcgccg cccggagacg aaaaacttct
     5041 ggcccaaatc gaaagcgaac tggctcgctt gggcctggct ccgaaaccgc aaaaggggca
     5101 actcccgatc cgtgcgtcga aacccgcgaa acctcaattc aaagagttta tcacctccga
     5161 aggattccgg gtcatcgtgg gacgtaataa tgaggagaat gacaagatca ccctggaggt
     5221 tgccaaaggt gacgacttct ggttccacgc ccagcagtgc ccgggcgcgc acgtggtctt
     5281 gaaactggac ggcaagcggg agccgggcaa acgctcgctt ttggaagccg ccgcgctggc
     5341 cgcccacttc tcggacgccc gccactcccg caaggtgccg gtgatttaca cgcgggcaaa
     5401 gtacgtccgc aagccgcgca aggccaagcc agggttaatg acggtggagc gggaaaaatc
     5461 gcttatggtg ccgccggcgc ttttgccatt gaaagccgaa taaattgatg acccctctct
     5521 gtcccttccc tccgagggga gggaagaatc tttcgctaca accgtagcgc aggttggttt
     5581 tggtaacctg cgcaagaccc ccgtgtcggt acgagtcgtg tcaacccgta aatgcggaac
     5641 gccctcccct ttgaggggag ggcggggagg ggtcaagaac ttcactgaga tacatggcct
     5701 aaagaacccg ccaaccggaa cgacccctcc agagtatcat tacttaggtt atgctgaagc
     5761 gagcaattgt tgcgctattc ggacttttag ccgtttccct tccgctcggc tttctcgttc
     5821 attcacagcc cgttagagtc acttccctca ccgatccgca gacctttacc gtcgcccggc
     5881 ttaaatatgg cggaggggga gactggtact ggggaccatc agccctcccc aatctcttgc
     5941 gctatattaa atagaacacc tcaattccgg tg
//
LOCUS       DBNGZD010000121         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_715530, whole genome shotgun sequence.
ACCESSION   DBNGZD010000121 DBNGZD010000000
VERSION     DBNGZD010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_715530"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="AC16GM_03295"
     CDS             complement(<1..1536)
                     /locus_tag="AC16GM_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2922734.1"
                     /translation="MTEDKSNRSPEFVLSNQKGREDARWPLTIICAIGILLQGCAATN
                     PPIKNKAVDAKQPKVKIELIPQEKLPRGVPAERATEYALRAILTSKEFAEFEKLKTRE
                     EQEKWVFRYWSERNPRPDRPQQNPYREEVLERILNALERFPAQGTPQPWDDRGEVWIR
                     QGEPKDRLIQESGSDSMWTKEVWTYPSIEYTFKDLNGTGEFRLTEAGYFGITPRSPSD
                     IAMTTSKMVATVRQQGVHYAYDYGKALKVIDRVEKFRQAEKSTGSDVFIQWAVRLRDL
                     LKPFSDTAKYSWRVVAYDLNGNVVCQDSASETRKITTPVDGQVFINQCRLWLPPGNYT
                     YALAVEGSGGLGLIKRKEPLIIPEYVTLRERSISDLMLPSLMKDVTGDSIPAELLRFV
                     RTFEGHEYLIVPSTGQIFQGDLLNLYAELYYLTLDTTTEKGVSRFRAKISLVSQKGQT
                     KRFLLADQELEGEGQHVPLLKAFSTEKIPPGEYIVNVEVEDLLAKPTDSKRKLTILGP
                     VKIN"
     gene            complement(1617..3305)
                     /locus_tag="AC16GM_03300"
     CDS             complement(1617..3305)
                     /locus_tag="AC16GM_03300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR2922735.1"
                     /translation="MIAGEKGLGNVIWAPDGKFIYFSSTEPKPEQKDQPAYERLNELY
                     ERWNYWKDKSHLNLISVVDKTRHHLTTGEYAIDDAALSPDGKKLALVRSVPIPERPFF
                     ATELWLFDPGARTEEKLANLRRQVGHLVWSPDGSALALSASVGEVTGDTSAVEHNNYN
                     YDLFLLSLSDRNLKNLTLNFDPHVGAALTGASDGRSPLWWSAQDKKIHFLATNRGRVN
                     LYRVDPAKPGIVEPLNLPFPVSSGTDVARNGRDLVFIGSSPTQMPQVYHYDLQRKKST
                     QVLTLNGELTTRLVLGKVEPWDFTNARGTKIDGWIYYPTDFAPDKKYPLVVYYYGGVF
                     PNGEAFGFTYHWWNAHGYLVYVLNPVGAVGYGQKFADLHVNDWGELAAADIIEGVQKL
                     SREKPCIDSTRVGAYGGSYGGFMTMTLATKTKLFGALCSMYGIADITSYWGEGWWGYL
                     YSDVATAKSYPWNRKDVYIARSPLYNADKITTPLLLLHGTGDTNVPPGESEQMFTALK
                     ILKQDVAYVRFPGEGHGISNKLSNYLSHRQMMLEWFDKYLKDQPEGWEERWKKK"
BASE COUNT          686 a          967 c          916 g          873 t
ORIGIN      
        1 gttgatcttc acgggcccca agattgttaa tttccgtttt gaatccgtag gcttcgccaa
       61 gagatcttcc acctcaacat taacgatata ctcaccgggc gggatctttt ctgtggagaa
      121 tgctttcaac aagggcacat gttggccttc accttcaagt tcctggtcgg caaggagaaa
      181 ccgtttggtt tgacccttct gagagactaa gctaatcttc gcccgaaaac ggcttactcc
      241 tttctctgtt gttgtatcaa gggtgagata gtagagctcg gcgtagagat ttaggagatc
      301 tccttggaag atttggcctg ttgagggaac aattaggtac tcgtggcctt cgaaagttcg
      361 aacaaagcga agtagttcgg ctggtattga atcgcccgtc acgtctttca tgaggcttgg
      421 gagcataaga tcacttatag aacgttcccg gagggtgacg tactcaggaa tgatgagtgg
      481 ttcttttcgc tttataagac cgagcccacc agaaccctcc acagccagag catacgtata
      541 gttccctggt ggaagccaga gccggcattg gttgatgaat acctgtccat caaccggtgt
      601 ggtgatttta cgggtttcac tcgcgctatc ttggcaaacg acattcccgt tgaggtcgta
      661 agcgaccaca cgccagctat acttcgccgt gtcgctgaac ggcttcaata ggtcacgcag
      721 acgaaccgcc cactggataa aaacatccga acccgtcgat ttttccgctt gccggaactt
      781 ttccacgcgg tctatgacct taagtgcttt cccatagtcg taagcgtagt gaacgccctg
      841 ctggcggaca gttgcgacca ttttggatgt cgtcatcgct atgtcgcttg gactgcgggg
      901 agtgattcca aagtacccag cttcggtgag cctgaactcc cctgttccat tcagatcctt
      961 aaacgtgtac tctatcgagg ggtatgtcca gacctccttc gtccacatac tgtcactccc
     1021 gctctcttgg atcagccgat cttttggctc cccttgacgt atccagactt caccgcggtc
     1081 gtcccacggc tggggcgttc cctgcgccgg gaatcgctcc aaggcgttga gaattcgctc
     1141 cagcacttct tctcgatagg ggttttgctg gggccgatcg ggccgggggt ttcgctcgct
     1201 ccagtaacgg aagacccact tctcctgttc ctctcgtgtt ttgagtttct cgaactctgc
     1261 gaattccttc gaggtaagaa tggctcgtag tgcgtactcg gtcgcccgtt ctgctggtac
     1321 accccgtggt aatttttcct gcgggattaa ttcaatcttg acttttggtt gtttcgcatc
     1381 aaccgccttg ttcttgatcg gcggatttgt agctgcgcat ccttggagca gtatccctat
     1441 tgcgcaaata atggtgaggg gccaacgggc atcctccctt cctttttgat tgctaagaac
     1501 gaattctggg cttcggttgc ttttgtcttc ggtcataccg tgttttccta cccccgtctc
     1561 catgacggca atctacgata ccccccagat ttgccaagta gttttggaaa cctgatttac
     1621 ttcttcttcc acctttcctc ccacccctcc ggctggtctt ttaagtactt gtcgaaccat
     1681 tccaacatca tctgtctgtg ggagaggtag ttgctcaatt tgttgctgat cccgtgcccc
     1741 tctcccggga agcggacgta ggccacgtcc tgcttgagga tttttagcgc cgtgaacatc
     1801 tgctccgatt cgcccggcgg gacgttcgtg tcgccggtgc cgtggaggag caaaagcggg
     1861 gtggtgattt tgtcggcatt gtagagaggc gagcgggcga tgtagacgtc tttccggttc
     1921 caggggtaac ttttggcagt ggccacgtca ctgtagagat aaccccacca accctcgccc
     1981 cagtaactgg tgatgtctgc gatgccgtac atggaacaga gggcaccgaa gagtttggtc
     2041 ttggtcgcca gagtcatcgt cataaagccg ccgtaactgc cgccgtaagc gccgacgcgc
     2101 gtcgagtcga tgcagggctt ctcgcgggat aacttctgca cgccttcgat gatgtcggcg
     2161 gcggccaact ccccccagtc gttcacgtgc agatcggcga acttctgccc gtaccccacc
     2221 gcgccgaccg gattgaggac gtagacgaga tagccgtgcg cattccacca atggtaggtg
     2281 aatccgaagg cttcgccgtt gggaaagact cccccgtagt agtagacgac cagcgggtac
     2341 ttcttatccg gcgcaaaatc agttggatag taaatccagc cgtcgatttt cgtcccgcgc
     2401 gcattggtga aatcccaggg ctcaactttg cccagcacca accgagtggt cagttcgcca
     2461 ttcaacgtta agacctgagt tgatttcttc cgctggagat cgtagtggta gacctgcggc
     2521 atttgcgtgg gcgaagaacc gataaagacg agatccctac cgttgcgggc cacatccgta
     2581 cctgaggaaa ccgggaaagg cagattgagc ggctcgacta tgcccggttt ggccggatcg
     2641 acgcggtaga gattcaccct cccccgattg gtagccaaaa agtggatttt cttatcctgc
     2701 gcactccacc agaggggcga gcggccgtcc gaggcgcccg tgagcgccgc accaacgtgc
     2761 gggtcaaaat tcagtgtgag atttttgaga tttcgatcgg aaagtgaaag caagaataag
     2821 tcgtaattgt agttattgtg ttcgaccgca ctcgtgtcgc cggtcacttc cccgacgctg
     2881 gcgcttagcg ccaacgcact gccatccggc gaccagacca ggtgccccac ctgccggcgc
     2941 agattcgcta gtttttcctc agtgcgtgct cccggatcaa agagccagag ttcggtggca
     3001 aaaaaggggc gctcaggaat cggcacgctg cgcaccagcg ccagtttttt cccgtccggt
     3061 gataaagccg cgtcgtcgat ggcgtactcc ccggtggtca ggtgatggcg cgttttgtcc
     3121 accaccgaga ttaaattcag gtgtgactta tccttccagt agttccagcg ctcataaagt
     3181 tcattcaacc tttcgtatgc cggctgatct ttttgctccg gcttcggttc ggtggaggaa
     3241 aaatagatga acttgccgtc cggcgcccag atgacgttcc ccagcccttt ctctccggcg
     3301 atcaaccggt caacccggcc tgaggtgatt tcgagcagcc agaggtcgtt cccctcttta
     3361 tcccctggcg cctggaaagc caggcgctct ccgtcgggct accagaccgg cgtggaaacg
     3421 ccgtctccca ttctttgcgt ct
//
LOCUS       DBNGZD010000122        13895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_507829, whole genome shotgun sequence.
ACCESSION   DBNGZD010000122 DBNGZD010000000
VERSION     DBNGZD010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 13895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13895
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_507829"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC16GM_03305"
     CDS             complement(<1..189)
                     /locus_tag="AC16GM_03305"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR2922736.1"
                     /translation="MGRNSSNNRYHRPAWVEVNLKQLAQNVRVIKKAIGLRSQLMAVV
                     KADAYGHGAVPVAKAALAA"
     gene            complement(192..1652)
                     /gene="dacB"
                     /locus_tag="AC16GM_03310"
     CDS             complement(192..1652)
                     /gene="dacB"
                     /locus_tag="AC16GM_03310"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR2922737.1"
                     /translation="MRRGTLAGGWLFFFIFELSTNAADYSALARTLDSLARAPELRGA
                     QVGIKIFCLDDSADVYEKNADQRMVPASNQKLVTSSCALVSLGRDFRFQTRFLSTGEI
                     REGTLKGDLIIEGRGDPSISDRDHSSNLEIFELWCDSLKSRGIRAIEGNLVLDERYFD
                     DQRVPAGWTWRDLSYWYATSTGALSYSDNCVALFFSPGPKVGAPAKIKIDPQTDYLTV
                     INQTKTGPRRTRRSIDYTRKPGTNVVTFFGQMGTRDTAEYKDYVSVEEPGKYLATVFR
                     QVLSSRRISVRGTIASAADDSAAVARAIPLFVWNSAPLAEILKVVNKNSQNFYAEQIL
                     KTVGREKRGEGSFAAGLAAESTFLLAVGIDSTQFDLSDGSGLSSDNLVTADALVRLLV
                     FMAHHPNRNEFYESMAIPGIDKTVKLRLNDVPQADQMRVKTGFIRHVSALSGYLAALN
                     GKGYAFSLLVNNYRGDLRRIWAWQDRFCRAVIEGAP"
     gene            complement(1718..2914)
                     /locus_tag="AC16GM_03315"
     CDS             complement(1718..2914)
                     /locus_tag="AC16GM_03315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR2922738.1"
                     /translation="MPQVITKERSALHFAERMSRLGTETAFEVLGRARQLEAQGRSVI
                     HLEIGEPDFDTPPNITDAAIVALKQKYTHYTPAAGLPEVREAVARYVAKTRKAPVSAD
                     QVVITPGAKPIITFSLLALIDAGDEVIYPNPGFPIYESVIRFCNGRPVPIRLREERNF
                     RLDSQELADLVTDRTKMIIVNSPENPTGSVLTRRDVEEICEIVRERDIWIFSDEIYSR
                     DLYGGEYTSPYSFPDMPERTIIMDGMSKAYAMTGWRLGYGVMPKELALQVTRLQINCT
                     SCTAAFSQRAIIEALEGPQESVAAMAAEFKRRRDVIVKGLNAIPRLSCQNPDGAFYVF
                     PNVSQIGWPSKKLADFLLEEAGVACLTGTSFGEFGEGYLRFSYANSIENIQEGLRRVE
                     SALKKL"
     gene            complement(2981..3940)
                     /locus_tag="AC16GM_03320"
     CDS             complement(2981..3940)
                     /locus_tag="AC16GM_03320"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR2922739.1"
                     /translation="MEWKEKKKTREGYGRALEELGERNPNVVVLTGDLAESTMAHFFA
                     QKFPERFFNMGVAEQNMANVSAGLSLTGKIPFFSTYGCFASCRALDMLRVTICYSNLN
                     VKIGGAHGGISVGPDGATHQAMEEFSIMRSMPNMHLVVPCDYWEAKKATLAAAEIRGP
                     VYIRFGREDVPVVTTEETPFVFGKGLELRPGHDVALLACGTMVSEALSAAEMLATEGI
                     DARVVNIHTVKPIDQEIIIKAARECGAVVTAEEHQINGGFGGAVAEVLVRNCPVPVEM
                     VAMNDRFGQSGKPEELLAAFHMKDVDIAKAAKKVLARKRNGKM"
     gene            complement(3986..4903)
                     /locus_tag="AC16GM_03325"
     CDS             complement(3986..4903)
                     /locus_tag="AC16GM_03325"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR2922740.1"
                     /translation="MNPELLVAEPPPKTERVWMNPFKYSVEQLKEKARIIRRNIITMN
                     VEAKSGHTGGPLSAADFCTVLFFHEMNFDPKSPKWPDRDMWFFSFGHVTPVHYSCLAE
                     AGFFPKCDLMKFRKFDGHLQGHPSSLDTPGVEVSSGSLGQGLSISVGAALGSRLDKHP
                     RRIYCLMSDGEQQEGSIWEAAMAGGHFRLDNLCGIIDYNRKQIDGDVEEVMGVAPLAD
                     KWRAFRWHVIEVDGQDIPQLLAAFDEARRIKGRPTVVLAHTVMGKGVSFMEDDFRWHG
                     KPPKPDEGERALAELGTTYAEWSRYLLEN"
     gene            complement(5137..5745)
                     /gene="coaE"
                     /locus_tag="AC16GM_03330"
     CDS             complement(5137..5745)
                     /gene="coaE"
                     /locus_tag="AC16GM_03330"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR2922741.1"
                     /translation="MILGLAGGIASGKTEVARVFQKLGAIIISGDDLGREVVEKDQRV
                     WVRLVETFGRKILNSNGTLNRRKLGQLAFSSPVSRKKLNRIVHPSLLRLMRQRVTYVR
                     RQNRKNPIVIDAALLVEWKIPVKMDKLIVVHASRNIRVRRLRKKDLTLSEIRDRMRSQ
                     LPFSVERKHADIIIRNDGTLEELRQKAREVWNQLIRSERGRH"
     gene            complement(5760..8456)
                     /gene="polA"
                     /locus_tag="AC16GM_03335"
     CDS             complement(5760..8456)
                     /gene="polA"
                     /locus_tag="AC16GM_03335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR2922742.1"
                     /translation="MKRERLFLIDGSALAYRAYFAFIRRPLINSKGENTSAAYGFVNS
                     LLKLLREEEPQYIALVFDTKAPTFRHQMYKEYKSTRAKMPEEMVAQLPRIRALAEALN
                     LPILEKEGYEADDLIGTLAVQAQKAGLAAVMVTGDKDFMQLVDDHVVILNPRQTTEEL
                     ELLDRAGVKEKIGVPPEKVIDFLGLMGDTSDNVPGIPGVGPKTAVELIEEFGSLEGVL
                     KNAEKIKKEKLRENILQHKEDALLSKKLVTIDTNAPVKLDLKHLRRQPPDEAALRKLF
                     LDLEFTSLLKILGGEGDGKKVVYRLVDDLSGLKSLVAVLKRAGEFAVDTETTNLEPLT
                     CELVGISISKKAGEAFYIPVGHTGPDAKKNLRLDETLALFRPLLESADLKKIGQNVKY
                     DYQVFRNHAIEMKGIACDTMLVSYLLNPTGRQHNLDALAQEHFGHKMIPISDLIGTGK
                     KQRSFAEVPVEKACEYSAEDADFTFRLKEKLLPRLAELGLEKLFYEIELPLVPVLAEM
                     ERNGVALDVPFLKKLSIELDKRLEKLTEKIYDLAGEKFNINSTQQLGQVLFEKLKLTP
                     GRKTTKKTALSTDIGVLEELAKEHKLPALIVEYRQYTKLKSTYIDALPALINPRTGRL
                     HTSFNQAVAATGRLSSADPNLQNIPIRTEVGSQIRKAFIPGEKGYSILSADYSQIELR
                     LMAHFSGDKNLIAAFERGEDVHARTASEVFGVPIDKVTSEQRRVAKTANFALMYGVTA
                     YGLSQQSEMTLQEAKQFIETYFARYPGVRQYMDDAIAQAQEKGYAETLFGRRRSLPEI
                     NSQNAQVRGFAERNAINTPLQGPAADVIKKAMIEVHERLKGKKSKMILQVHDELVFEV
                     PPDEVEWLKKMVTKEMEEVVKLKVPLKVDIGVGKNWLETKEG"
     gene            complement(8463..9062)
                     /gene="nadD"
                     /locus_tag="AC16GM_03340"
     CDS             complement(8463..9062)
                     /gene="nadD"
                     /locus_tag="AC16GM_03340"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR2922743.1"
                     /translation="MTRATPIGLFGGTFDPIHTAHLIIAQEAVTQLDLARLYFIPAFV
                     PPHKKGKRVTDFAHRLRMVALATAGNPRFACSEIELRRGGLSFSVDTVAAFRRQLPGA
                     RLHFLLGADNLKDLRFWHQPKKLFSLCRVVMVNRPGSFHPRIRDPFIRRILWLDAPQM
                     DISATHIREKVRRGETIRYLVPDAVAAYITKHRLYRSHA"
     gene            complement(9059..9874)
                     /locus_tag="AC16GM_03345"
     CDS             complement(9059..9874)
                     /locus_tag="AC16GM_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR2922744.1"
                     /translation="MRLRTYIFGSSAFFLAALFFVFACGRKATKEVPGQEALLAKALS
                     LFQRKKYLDATAEFQKYIFNYPGGTGVDTAEFYLGLSYFHQNDFLLAVAELRKIPSAY
                     PTSAFADDAQFYLGESHFKLAPGSVGTDQSEIDSAIVAYEGLLEEHPTTEYHEKAQAQ
                     LLRCRDRLARKAFETALLYYKLYDFRAAAISFQDFLDNHHQSRYAPEALYYLGISLEK
                     SGKASAACEKFRAYLVLFPSEKRGKEIQGIVAKFCPSEQPETATPADTTRPRP"
     gene            complement(9874..10500)
                     /locus_tag="AC16GM_03350"
     CDS             complement(9874..10500)
                     /locus_tag="AC16GM_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922745.1"
                     /translation="MKQFLLLSILLTSRLTLAQTAPPANEIFLRQAVSDVWDSLLAGQ
                     LSQPFVWHLVSDSGHPGNWLVEAALIEKLQAQHLQVRSGISLAADSTATSQLVRLLGY
                     RIIDLSLAYPRIWRKGFTGRKELRRQARVALSARLSDPATGEILFDRRASVIREDQLT
                     SAQVREVENGNYPLLSPPVPPLGYNKYIEPIVVTGVVAALVLLLFTSR"
     gene            complement(10562..11218)
                     /locus_tag="AC16GM_03355"
     CDS             complement(10562..11218)
                     /locus_tag="AC16GM_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015510.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR2922746.1"
                     /translation="MSLPKRFGLCLRGGVVVSLLAVPHPAFGLSGSQIADSVKQRFTQ
                     AKTIFVQYRQETRQKGFATAGKITGKIWLAGEKKFRIENPEQTVVSDGESLWSYAPRL
                     KQVTIQKLSKVKNLVLPKDFLFSFFNDYSIATSGEETVAGKPCFRLTLKNLKPDMLIA
                     VMTVWVEQGSFFTRKVAYTDANDTRVTFSFPTIRTDVAVPGSRFTFAAPQGVEVVTLP
                     "
     gene            complement(11224..13536)
                     /locus_tag="AC16GM_03360"
     CDS             complement(11224..13536)
                     /locus_tag="AC16GM_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR2922747.1"
                     /translation="MEKERRKRTLTLRRLKLPRREVGGLLLFAVAVLMLLSLVTFDRL
                     RDRYALAQVDWTHPSSLWELRVPNQGGIVGAYVASGLYNAFGFLALFLPLYLGSWGAS
                     LTLKRSWPLFFKKTTLLLATFFFVACVAALSRGQAERLNETITLGGLFAQNAARFCTG
                     LFGVAGSATIFIAGALVLVTILFPLDWGKLSSHLRLPARPKLFWHTAAIRWGRAWEHL
                     KSRRQAEGEQSLSSPTRPQVVLREAAADPPIGAPPRPARVKKAEGPEELNLFSELQKT
                     LNIVKGPELPPEGDYRFPGVDLLNLPSVIKSAVSRDELEQTAQALKQTLETFNVQIEG
                     EAIEAYPGPVITRFEFKPAPGIKVNQIVSLADDLALAMQAKSIRIIAPSTGKAAVGVE
                     IPNRQPQTVYLREIISSGLFQRPERRLPLALGKTIGGEPFVADLSKMPHLLIAGATGS
                     GKSVCLNGIITSLLFRLHPDLLRFVFIDPKMLELSIYQGIPHLLRPVVTTPRQAEKVL
                     QEAVVEMEDRYRKLAQAAVRNIEDYNAKKKRADRLPYLLIVVDELADLMMSTNSARTV
                     MFVARLAQMARAVGIHLLLATQRPSVDVITGVIKANFSSRIAFQVASKGDSRVILDVN
                     GAEKLLGAGDMLFLQTGQPEPVRIHGAYISGQETEQIVNFLKSQTYQPVAAETPLVQT
                     SSPDLETDDTDEDADQLFHQAAELVIRHGQGSVSLLQRRLGIGYQRAARLIDQLEAAG
                     LVGPYVGSKTREVLVDKSYLERFTSPPAQS"
     gene            complement(13645..13890)
                     /locus_tag="AC16GM_03365"
     CDS             complement(13645..13890)
                     /locus_tag="AC16GM_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR2922748.1"
                     /translation="MSKEAAISKAQAGRALDALFKGVTGSLKQGRRVSFVGFGTFSVG
                     KRKARMGRNPQTGATIKIAAARVPKFKAGKALKAAVK"
BASE COUNT         2865 a         4006 c         4024 g         3000 t
ORIGIN      
        1 tgccgccagc gccgcttttg caacgggaac agccccgtgg ccgtaggcgt cggctttgac
       61 caccgccatc aactgcgagc gcagcccaat tgcctttttg atgacccgaa cgttttgcgc
      121 gagttgcttt aagttaactt cgacccaggc aggccggtga taccggttgt tggaagagtt
      181 ccggcccatt gctacggcgc tccctcgatg accgcccggc agaagcggtc ctgccaggcc
      241 cagatccgcc tcaggtcgcc ccggtagttg ttgacgagaa gcgaaaaggc gtaccccttg
      301 ccgttgagag ccgcgaggta tccggagaga gctgagacgt gccggatgaa gccggtcttg
      361 acccgcatct ggtctgcttg gggaacgtcg ttcagccgga gtttgacggt cttatcgatg
      421 ccgggtatgg ccattgattc ataaaattca tttcgattcg ggtggtgcgc catgaaaact
      481 aagagtcgta ccagggcgtc ggcggtgacg agattgtcgc tggaaaggcc cgaaccgtcg
      541 gagagatcaa actgcgtgga gtcgatgccg acggccagaa ggaaggtgct ctccgcggcc
      601 agtcccgcgg cgaaacttcc ctccccccgc ttttcccgcc ccacggtctt gaggatctgc
      661 tcggcgtaga agttctggct gttcttgttc accactttca gaatttcggc caaaggcgcc
      721 gaattccaca caaagagggg aatcgcccgg gccaccgccg ccgagtcgtc agcggcagaa
      781 gcaatagtgc cccgcacgga aatgcggcgc gaactcagga cctggcggaa caccgtcgcc
      841 agatatttcc ccggctcttc caccgaaacg taatccttgt actcggcggt gtctctcgtc
      901 cccatctgtc cgaagaaagt gacaacgttc gtgcccggtt tgcgggtgta gtcgatggaa
      961 cggcgtgttc ggcgcgggcc ggtcttcgtc tggttgatga cggtgaggta atcggtctgc
     1021 ggatcgattt tgatcttcgc cggcgccccc actttcgggc cgggagagaa gaagagcgcc
     1081 acgcagttgt cactgtacga aagcgcaccg gtagaggtgg cataccagta ggagaggtcg
     1141 cgccaggtcc agccggcggg caccctctgg tcgtcaaagt agcgctcatc taacaccaga
     1201 ttgccctcaa tggcgcgaat gccgcggctc tttagactgt cgcaccagag ttcaaaaatt
     1261 tcgaggttgg aactgtgatc gcgatccgag atcgaaggat cgccccggcc ctcaataatc
     1321 agatctcctt tgagcgtgcc ttcccgaatc tcgccggtgg agagaaacct cgtttggaag
     1381 cggaaatcgc gacccaaact gaccagagcg caggacgacg tgaccagttt ctgattcgag
     1441 gcgggaacca tccgctggtc ggcatttttc tcatagacat ctgcactgtc atccaagcag
     1501 aaaattttga tgcccacctg cgccccgcgc agttccggtg cgcgtgccag ggaatcgagc
     1561 gtgcgggcta aagcggaata atccgccgca tttgtcgaca gttcaaagat gaaaaagaaa
     1621 agccacccac ccgcgagtgt tccccgcctc atgttcttaa atatacggaa atgccgggta
     1681 actggtagaa atgaaggagt gtccgaaaaa acaaccgcta caacttcttg agtgccgact
     1741 ctacgcggcg gagtccttct tgaatatttt cgatggaatt ggcgtaggag aagcgcaggt
     1801 aaccctcgcc gaactccccg aacgacgtgc ccgtcaaaca ggcaacgcct gcttcttcga
     1861 ggaggaaatc ggctaatttc ttgctcggcc acccgatttg ggaaacgttg gggaaaacgt
     1921 agaaggcgcc gtcgggattc tggcaactga ggcggggaat ggcgttgagt cctttgacaa
     1981 tcacatcgcg tcggcgtttg aattcggccg ccatggcggc gaccgattcc tgcggccctt
     2041 ccagcgcttc tatgatggcg cgctgggaaa aagccgcggt gcaggaagtg cagttgattt
     2101 gcaggcgcgt cacctgcagg gccaactctt tcggcatgac gccgtacccc agccgccagc
     2161 cggtcatggc gtaggctttc gacatcccgt ccatgatgat cgtccgttcg ggcatatcgg
     2221 ggaaagagta aggggaggtg tactcgccgc cgtagaggtc gcggctgtag atttcatcgg
     2281 aaaaaatcca gatgtcgcgt tcgcggacaa tttcgcagat ttcctcgaca tcgcgccggg
     2341 tcaggacgct gccggtaggg ttttccgggg agttgacgat gatcatcttc gtccggtcgg
     2401 tgacgagatc ggctaactcc tgtgagtcga gtcggaaatt tcgctcctcg cgcaggcgga
     2461 tcggcacggg ccggccgttg cagaaacgaa tgaccgactc gtagatggga aagccgggat
     2521 tgggatagat gacctcatcg ccggcgtcga tgagcgccag aagcgaaaag gtgataatcg
     2581 gcttggctcc cggggttatc accacctggt cggcggagac gggcgccttg cgcgttttgg
     2641 ccacgtaccg tgccaccgcc tcgcgcactt cgggcaggcc ggcagcgggc gtgtaatggg
     2701 tgtatttctg tttcagtgct acaattgctg cgtcagtgat attagggggt gtatcaaaat
     2761 ccggctcgcc aatttctaag tgaatgactg agcgcccctg cgcttctaac tgccgggccc
     2821 ggcccaggac ttcaaaagcg gtctcggtcc cgaggcggga catccgttcg gcgaagtgaa
     2881 gcgctgatct ttctttggtt attacttgtg gcatatcaca ttctattcca attgattact
     2941 gaaatttgta aaagcgactc tttaagtccc aaccaaaggg ttacattttt ccattccgct
     3001 tacgggccaa aactttcttc gccgcttttg cgatgtcgac atctttcatg tgaaaagcgg
     3061 ccaagagttc ttccggtttt cccgactggc cgaagcggtc gttcatcgcc accatttcca
     3121 ccggcaccgg acagttgcgg acgagcactt cggctaccgc gccgccgaag ccgccgttga
     3181 tctggtgctc ttcggcggtg acgacggccc cgcactcccg cgcggccttg atgatgattt
     3241 cctggtcaat cggcttgacc gtgtgaatgt tcaccacgcg agcatcgatg ccctctgttg
     3301 ctaacatttc cgcggcggaa agtgcttcgg aaaccatcgt cccgcaggcc agaagtgcca
     3361 cgtcatggcc gggccggagt tccaagcctt tgccgaaaac aaacggcgtc tcttccgtgg
     3421 tgacgaccgg cacatcttcc cggccaaagc ggatgtaaac cggtccccga atttccgcgg
     3481 cggccagggt tgctttcttc gcctcccagt aatcacaagg caccaccagg tgcatattgg
     3541 gcattgagcg catgatggag aattcttcca tcgcctggtg agtggcgccg tccggaccca
     3601 cggagatgcc gccgtgcgcc ccgccgatct tgacgttcaa gttcgagtag caaatcgtga
     3661 cccggagcat atcgagcgcc cggcaggaag caaagcagcc gtaggtggag aagaaaggga
     3721 ttttcccggt caaagagagt ccggcggaga cgttggccat attctgctcc gccaccccca
     3781 tgttgaagaa gcgctccggg aatttctggg cgaagaagtg ggccatggtc gattcggcca
     3841 ggtcgccggt gagcaccacc acgttggggt tgcgctcgcc caattcttcc aacgcccgtc
     3901 cgtatccttc ccgcgtcttt ttcttttctt tccactccac cgttttatcc ctcggtcctc
     3961 atccgtctca ggggcggact gaattttaat tttctaagag gtagcgcgac cactccgcat
     4021 acgtcgttcc cagttcggcc agcgcccgtt cgccctcatc cggtttgggc ggtttgccgt
     4081 gccagcggaa gtcgtcctcc ataaaggaaa cgcctttccc cattaccgtg tgggccagaa
     4141 ccaccgtagg ccggcctttg atccgccggg cttcgtcgaa cgccgccaaa agctgcggaa
     4201 tgtcttggcc atcgacttca atcacgtgcc agcggaaagc gcgccatttg tcggctaaag
     4261 gcgccacgcc cattacctcc tccacgtcgc cgtcgatttg cttgcggttg tagtcgatga
     4321 tcccgcagag gttgtccagc cgaaagtgtc ccccagccat cgccgcttcc cagatcgagc
     4381 cttcctgctg ttcgccgtcg gacatcaagc agtaaatccg gcgcgggtgc ttatccaagc
     4441 gggagccgag cgccgcgccc acagaaatcg aaagcccctg acccagcgac ccggaggaaa
     4501 cttccacgcc cggagtgtcc agagaggagg gatgcccctg caggtggccg tcaaatttgc
     4561 ggaacttcat caggtcgcat ttcgggaaaa agcccgcttc agccagacag gagtagtgca
     4621 ccggtgtgac gtggccgaag gagaaaaacc acatgtcgcg gtcgggccac ttggggcttt
     4681 tcggatcgaa gttcatctcg tggaagaaga gcaccgtgca gaagtcggct gccgaaagcg
     4741 gcccgccggt gtgccccgac ttggcttcca cgttcatggt aatgatgttc cgccggatga
     4801 ttctggcttt ttcttttagc tgttcgacgg aatacttgaa gggattcatc cagacccgtt
     4861 cggtcttggg cggtggctcg gctaccaaca gttccggatt cattcagtct cgcttgtgaa
     4921 aagtgtcaca ttcactacca atatacgcca ggcgaaaaga aatgcaaaag gaatatttgt
     4981 cttaaactac ctcctggtgg cgcggaatgg ttctcatatt ccgcgccgcg caggttgcgg
     5041 ttcgacttcg ctcaccgcga gccaaaaccc ttcggcctcg ctcagggcag gccagcctgt
     5101 gcctccgtat ttgaaaaacc ccacgttggc ggcactttag tgccgccctc tttctgaacg
     5161 aatcaactga ttccaaacct ctcgggcttt ctgtcgcagc tcttccaatg tcccatcgtt
     5221 gcgaatgata atgtcggcgt gcttccgctc aaccgaaaaa ggcaattgtg agcgcatacg
     5281 gtcacgaatt tcagaaaggg ttaggtcctt ttttctgaga cgacggacgc ggatgttccg
     5341 ggaggcgtgg accacaatca gtttgtccat tttgaccggg attttccact cgactaagag
     5401 tgccgcgtca ataacgatag gattcttccg attttgcctc cgcacgtacg ttacacgttg
     5461 gcgcatcaac cgcaagaggg acgggtgcac aatccggttc agttttttgc ggctgaccgg
     5521 agaagaaaaa gccagttggc ctaacttccg gcggttaagc gtgccattgg aattgagaat
     5581 tttccgacca aacgtttcaa ccaaacgcac ccatacacgc tggtcttttt ccaccacttc
     5641 ccggcccaag tcatcgccgg aaatgatgat tgcaccgagt ttctggaaga cccgggcgac
     5701 ttcagttttg ccggaggcga tgccccccgc caggccgagg atcatggagt caggggaaat
     5761 tacccttcct tcgtctccaa ccagttcttg cctacgccaa tatcgacctt tagcggcact
     5821 ttcaatttga cgacctcttc catttccttc gtcaccattt tcttgagcca ctccacctca
     5881 tctggtggca cttcaaagac caactcgtca tggacttgca agatcatttt tgattttttg
     5941 cctttcagtc gctcgtgcac ttcgatcatc gctttcttga tgacatccgc cgcggggccc
     6001 tgcagggggg tgttgatggc gttccgctcg gcaaagccgc gcacctgtgc gttctgggaa
     6061 ttgatctccg gcagggaacg ccgccggccg aagagcgtct ccgcgtaccc tttctcctgc
     6121 gcctgggcga tggcgtcgtc catatactgg cgtacccccg ggtagcgggc aaaataggtt
     6181 tcgatgaact gcttggcttc ctgcagggtc atctcactct gctgggatag accgtaagcg
     6241 gtcaccccgt acatcagggc aaaattggcg gttttggcca cccgccgctg ttcggaggta
     6301 actttatcaa tcggcacacc gaacacctcc gaggccgtgc gggcgtggac gtcttcgccc
     6361 cgctcaaagg cggcgatgag gttcttatcg ccggagaaat gggccattaa ccggagttcg
     6421 atctgagaat agtcagccga gagaatggag taccctttct caccgggaat gaacgccttg
     6481 cggatttgac tgccgacttc ggtgcgaatc ggaatgttct gcaagttggg atcggctgag
     6541 gaaagccgtc cggtagcggc caccgcctga ttgaacgagg tgtggagtcg cccggtccgg
     6601 ggattgatga gtgcggggag ggcatcgatg taggtcgatt tcaatttcgt atattgccga
     6661 tactccacga tcaacgcggg cagtttgtgt tctttggcca gttcttctaa aaccccaatg
     6721 tcggtggaga gtgcggtttt cttagtggtc ttccgccccg gcgtcagctt taacttctca
     6781 aacaaaacct gacccagttg ctgggtggaa ttgatgttaa acttctctcc ggccaggtca
     6841 tagattttct ccgtcaattt ctccaaacgt ttatccaact cgatggagag ttttttgaga
     6901 aagggcacat ccagggccac accgttccgc tccatttcgg ccagcaccgg cacgagcggc
     6961 agttcaatct cgtagaaaag tttttccaat cccaattcgg ccaggcgcgg aaggagtttc
     7021 tctttcaacc ggaaggtgaa atcggcgtct tcagcggaat attcacaggc cttctcaacc
     7081 ggcacctcgg cgaaggatct ctgctttttc cccgtcccga tcaaatcgga aatgggaatc
     7141 atcttatggc cgaagtgttc ctgagccagt gcgtccagat tgtgctggcg gcccgtgggg
     7201 ttcaacaggt aggataccag catcgtatcg caagcaatcc ccttcatctc aatagcgtgg
     7261 ttccggaaaa cctggtaatc gtacttgacg ttctgtccga ttttttttaa gtcagccgat
     7321 tccaggagcg gacggaaaag ggccagcgtc tcgtccaaac gcagattttt cttcgcatcg
     7381 ggccctgtgt gtccgacggg aatgtagaag gcctctccgg ctttcttgga gatcgagatg
     7441 ccgaccagct cgcaggtgag cggctccaga ttcgtcgttt cggtatcgac ggcaaactct
     7501 ccggctctct tgagcaccgc aaccagactt ttgagtcccg agaggtcgtc caccagccgg
     7561 taaacaacct ttttcccatc cccttccccg cccagaattt tcaagagcga ggtgaattca
     7621 agatcaagga agagttttcg cagcgcggcc tcatccggcg gctggcgccg gagatgtttt
     7681 aggtcgagtt tgacaggggc gttggtatca atggtcacta atttctttga aagcaacgca
     7741 tcttctttat gctggagaat attctcccgc agtttttcct ttttgatttt ctccgcattt
     7801 ttcaggacac cctccaggga accgaactcc tctatgagtt cgactgcggt cttgggaccc
     7861 acgcccggaa tgccggggac attgtcggag gtgtcgccca tcagacctaa aaagtcaatg
     7921 accttctccg gcggcacccc gattttctct ttgactcccg cgcgatccag aagttcaagt
     7981 tcctcggtgg tctgccgcgg attgaggatc actacgtggt catccaccag ttgcatgaag
     8041 tctttgtctc cggtgaccat gacggcggcg agtcccgctt tctgcgcctg caccgccagc
     8101 gtgccgatca ggtcatcggc ttcatagcct tccttttcga ggatggggag attgagtgcc
     8161 tcggccaaag cccggatgcg cggcagctgg gcgaccatct cctccggcat cttcgcccgg
     8221 gtcgatttgt actccttgta catctggtgc cggaacgtgg gcgctttggt gtcgaagact
     8281 aacgcgatgt actggggttc ttcttcgcgg agaagtttca aaagcgaatt cacaaagccg
     8341 taggccgccg acgtgttctc ccctttggaa ttgatgaggg gccgccggat gaaggcgaaa
     8401 taggcacggt acgctaaggc cgagccgtca atcaaaaaga ggcgctcgcg tttcatagac
     8461 ccctatgcgt gtgaccggta gagccggtgc ttggtaatgt acgcggccac cgcatccgga
     8521 acaaggtagc ggatcgtctc gccgcgccgg actttctccc ggatatgcgt ggccgatatg
     8581 tccatttgcg gcgcatccag ccagagaatt ctgcgtatga agggatcgcg gatgcggggg
     8641 tgaaaactcc cgggccggtt caccatcacc acccggcaga gggaaaagag tttcttgggc
     8701 tggtgccaga agcgcaaatc tttcaagttg tccgctccca aaaggaagtg taaccgcgcg
     8761 ccgggaagtt gtcggcgaaa ggccgccacc gtatcgacag agaacgacag ccccccccgt
     8821 ctcaattcga tttcggaaca ggcaaagcgc ggattgccgg ctgtggccaa ggccaccatg
     8881 cgcaagcggt gggcgaagtc ggttacccgc ttgccctttt tatggggtgg cacaaaggcc
     8941 ggaatgaaat agagccgcgc taaatctaac tgggtgaccg cttcctgggc gatgattaaa
     9001 tgcgcggtgt ggatcggatc gaaggtcccg ccgaagaggc cgatgggagt ggcgcgggtc
     9061 atggccgcgg gcgggtcgta tcggcgggcg tggcggtctc gggttgctcg gagggacaga
     9121 actttgccac gatgccctga atttccttcc cccgcttctc actcggaaag agcaccaggt
     9181 aggcacggaa cttttcgcac gccgcgctcg ccttccccga tttctccagc gaaataccga
     9241 ggtagtagag cgcctccggg gcgtaacggc tctggtggtg gttgtccaga aaatcctgaa
     9301 acgagatcgc cgccgcccgg aaatcgtaca gcttatagta gagcagcgcc gtctcgaacg
     9361 ctttccgggc taagcgatcc cggcagcgga gtaactgggc ctgcgctttt tcgtggtact
     9421 cggtcgtggg gtgctcttcc aaaagccctt cgtaggccac gatggctgaa tcgatctcgc
     9481 tctggtcggt gccgaccgag ccgggcgcca gcttgaagtg gctctctccc aagtaaaact
     9541 gcgcgtcgtc ggcaaaggcg ctggtggggt aggccgaggg aatcttgcgc aattcggcaa
     9601 cggccaagag gaagtcgttc tggtggaagt acgacagtcc cagatagaac tcggccgtat
     9661 cgacacccgt ccccccggga tagttgaaaa tgtatttttg gaattccgcg gtggcatcca
     9721 ggtatttctt ccgctggaaa agtgagaggg ctttggccaa aagcgcttcc tgtcccggta
     9781 cttcttttgt cgctttgcgc ccgcaggcaa aaacgaaaaa gagcgccgcc agaaagaacg
     9841 cgctgctgcc gaaaatgtag gtacgcagcc tcactaacga ctcgtaaaga ggagcaggac
     9901 taaggccgcc accacgccgg tcaccacaat cggctcaata tacttattat accccaaggg
     9961 gggcaccggc ggagacaaaa gtggatagtt cccgttctcc acctcccgca cctgggcact
    10021 ggtcagttga tcttcccgga tcacgctcgc gcgccggtcg aacagaattt caccggtagc
    10081 cggatcgctg aggcgggccg aaagcgccac ccgggcctgc cggcgtaact cctttcggcc
    10141 ggtaaatccc ttgcgccaga ttctgggata cgcgagggac agatcaatta tccgataccc
    10201 taatagccgc accaactgac tggttgccgt actgtccgca gcaagtgaaa tgcccgaccg
    10261 cacctgcagg tgctgcgcct ggagcttttc tatcaacgcc gcctccacca gccagttccc
    10321 gggatggccg gaatcggaaa ccaggtgcca gacgaatggc tgcgagagtt gaccggccaa
    10381 aagcgaatcc cacacatcgc ttaccgcttg gcgcaggaaa atttcattgg caggtggtgc
    10441 cgtttgggcc aaagtcaaac gggaagtcaa aagaatcgaa aggagcagga actgcttcac
    10501 gcgacccgct cccgtactgc aatctcttcc tctccggaag tgataacgcg cctgatcatc
    10561 gctaaggaag agtcactact tccacgcctt gcggcgccgc aaaggtgaac cgggatccgg
    10621 gaacggcaac atcggtgcgg atggtcggga acgagaaagt aactcgagta tcattggcgt
    10681 ccgtgtaggc gactttccgc gtaaagaaac ttccctgctc cacccaaacg gtcatgacgg
    10741 caatgagcat gtctggtttt aggttcttca aagtcaatcg gaagcagggc tttccggcaa
    10801 ccgtttcttc accgctggtg gcaatgctgt agtcattgaa aaacgaaaag agaaaatctt
    10861 tgggcagaac gagatttttg actttggaca acttttggat ggtcacctgc ttcagccgcg
    10921 gcgcatacga ccagagactt tccccgtcgg aaaccaccgt ctgctccgga ttctcgatgc
    10981 ggaacttctt ttctcccgcc agccagattt tgccggtgat tttcccggcc gtggcaaacc
    11041 ccttctggcg ggtctcctgc cgatactgga caaagatggt tttggcctgt gtaaagcgct
    11101 gtttgaccga atcggcgatc tgggaaccgg aaagtccgaa agccgggtgt ggtactgcca
    11161 gaagcgagac gacgacgcca ccgcgcaggc aaagaccgaa tcgtttggga agggacatcg
    11221 ggattagctc tgagccggtg gactggtgaa gcgctccagg tatgatttgt cgaccagcac
    11281 ctcgcgggtc ttgctgccga cgtacggccc cacgagcccc gcggcttcga gctgatcaat
    11341 gagccgcgcc gcccgttggt acccgatccc caaccgccgc tgcaaaagcg agaccgagcc
    11401 ctgcccgtgc cggatgacga gctccgccgc ctggtggaac agttgatcgg cgtcctcgtc
    11461 tgtgtcgtcg gtctccagat cgggtgagga cgtctgcacc aaaggcgttt cggcagccac
    11521 cggctgatag gtctggctct tgaggaagtt gacgatctgt tcggtttcct ggccggaaat
    11581 gtaggcgccg tggatgcgga ccggctccgg ctggccggtc tgcagaaaga gcatatcccc
    11641 cgcacccaaa agtttttcgg cgccgttcac atctaagatg acgcgcgaat cgcctttgga
    11701 agccacctgg aaggcgatcc gggaggaaaa gttggctttg atcacgcccg tgatgacgtc
    11761 gaccgacggc cgctgcgtgg ctaaaagcaa gtgaatcccc accgcccgcg ccatctgcgc
    11821 cagacgggcg acgaacatca ccgtccgcgc cgaattggtg ctcatcatca ggtcggccag
    11881 ttcgtccacg acgatcaaaa ggtacgggag gcggtcggcc cgtttcttct tggcattgta
    11941 gtcctcgatg ttccgcacgg cggcctgcgc taatttccgg tagcgatcct ccatttcaac
    12001 cactgcttcc tgcaacacct tctccgcctg acgcggggtg gtgactaccg ggcgcaatag
    12061 gtgcgggatg ccctggtaga tagagagctc taacatcttg ggatcgatga aaacgaagcg
    12121 caaaagatcc gggtgcaggc ggaagaggag actggtgatg atgccgttca aacagacgct
    12181 tttgcccgag ccggtggctc cggcgatgag caggtggggc atcttggaga gatcggccac
    12241 gaacggctcg cccccgatcg ttttccccaa ggccaggggc agccgccgct ccggtctttg
    12301 aaacaatccc gaagaaatga tttcgcgcag gtagaccgtc tgcggctggc ggttggggat
    12361 ttcaaccccc accgccgctt ttcccgtact cggcgcgatg atccggatgc tcttggcctg
    12421 catggccaga gctaaatcgt cggccaaact gacaatctgg ttgaccttga ttccgggggc
    12481 cggcttgaac tcaaagcggg tgatgaccgg cccgggatag gcttcgatcg cttcgccttc
    12541 aatctgcaca ttgaaggttt cgagcgtttg cttgagcgcc tgggccgttt gctctaattc
    12601 atcgcgggag accgcgcttt tgatgaccga agggaggttc aaaagatcga ctccgggaaa
    12661 acggtagtcg ccttccggcg ggagttcggg ccctttgacg atgttaaggg ttttctgcaa
    12721 ttcggaaaac aaattgagtt cttcgggccc ttcggctttt ttcacccgcg ccggacgcgg
    12781 tggcgccccg atcggcgggt cagcggctgc ctcgcggagc acaacctgcg gtcgcgttgg
    12841 ggatgaaagc gactgctccc cctccgcctg ccgccgggat ttcaaatgtt cccacgcgcg
    12901 tccccagcga attgcggcgg tgtgccagaa gagcttcggt cgggccggca ggcgaaggtg
    12961 actggagagt tttccccagt cgagggggaa aagaatcgtc accaggacca gcgctccggc
    13021 gataaaaatc gtggccgacc cggctacccc aaaaagaccg gtgcagaaac gcgccgcgtt
    13081 ctgggcgaaa aggccgccta aagtgatggt ttcattcaac cgctccgcct gcccgcgcga
    13141 cagcgccgcc acgcaggcga cgaagaagaa cgtcgccaaa aggagagtcg ttttcttgaa
    13201 aaagagcggc caggagcgtt tgagcgtcag actggcgccc caggaaccga ggtagagcgg
    13261 caggaagagc gccagaaagc cgaaggcatt gtaaagcccg gacgccacgt aggcaccgac
    13321 aattcctccc tggttcggca cccggagttc ccagagactg gagggatgcg tccaatcgac
    13381 ctgggccagg gcgtagcggt cacgcagccg gtcgaaagtg acgagactca agagcatcaa
    13441 aaccgccacc gcaaaaagga gtaggccgcc gacttcgcgg cgcggtaact tcagccggcg
    13501 gagagtgaga gtccttttcc ttctttcctt ctccatggat aaggagaatc taacatcggc
    13561 gtgcgcccgg cgtcaataaa aaagccccgc cgtggcgggg cttcacactg tcagcagagt
    13621 gaaagctctg ccgccatggg agaattactt taccgcggcc ttgagggctt ttccggcttt
    13681 gaactttggc acccgcgccg cagcgatctt gatggtggcg ccggtctgcg ggttgcggcc
    13741 catacgcgct ttccgctttc ccactgagaa agtgccgaaa ccgacgaacg agacccgcct
    13801 accctgtttg agcgaaccgg tcacaccttt gaagagggcg tccagtgccc ggccggcctg
    13861 cgccttggaa atggcggctt ccttggagat cctgg
//
LOCUS       DBNGZD010000123         3159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1270966, whole genome shotgun sequence.
ACCESSION   DBNGZD010000123 DBNGZD010000000
VERSION     DBNGZD010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1270966"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="AC16GM_03370"
     CDS             complement(<1..1055)
                     /locus_tag="AC16GM_03370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdM family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HIR2922749.1"
                     /translation="MEENEYKNYSTLEGLFEGKSKTGPGSGKPDFLLVESPESLRPLI
                     VIDTKATLALFKDAVKDANHYGNACFDSGIEVLSVAVAGAEREICSVKVQRRVNGNWK
                     DLTLHGSPIDWIPSPRQTISILASRNRIEVEPERPSEEVLASQANKLNEILRECKIKD
                     EYRPIYVATFMLALWKGNVSTDKEVVLTQVNANAEQALQKAGKPELAHSLRVDDENDA
                     LAARAWEIIDILKKLNIRSFMQEHDYLGQLYETFFRYTGGNTIGQYFTPRHIISFMCE
                     LVDVTPQDIVFDPACGTGGFLIGALNRMIKKKSIPYEEGVRKVKNNLFGIESEPATAA
                     LCITNMILRGDGKSGVI"
     gene            complement(1259..2422)
                     /locus_tag="AC16GM_03375"
     CDS             complement(1259..2422)
                     /locus_tag="AC16GM_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-hypusine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HIR2922750.1"
                     /translation="MTQNNNPAATYLPSLPADKRQKKNKILTLPIEPARLDSNISVAD
                     LVDQMAKMSVQARNVGQCARVWQKMLDDPRRPTILLGLAGPLIAAGLRKVIRDLVVGG
                     YVDVIVSTGAILYQDIYQARGFKHFRGSPNADDKVLRELYINRIYDTYVDEEKFWETD
                     LWCGKKADELAPARVSSRFYLDHLGSKLDDDESIVAQCHRLGVPIFAPAINDSSIGIG
                     LTEHYHRMAQKKKPGMALDSIRDNYELTQIVVKSAETAAIYVAGGVPKNYINDSVVMG
                     YIFGRQRGHAYALQVTTDSPHWGGLSGSTLGEAQSWGKIDLDADFAMAFVEPSVSLPL
                     LAAYVMARCDTKARGRLRYEWQEDRLVGLTVVRDKEKPMPQRQESKKRAAVRV"
     gene            complement(2443..3033)
                     /gene="clpP"
                     /locus_tag="AC16GM_03380"
                     /pseudo
     CDS             complement(2443..3033)
                     /gene="clpP"
                     /locus_tag="AC16GM_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
BASE COUNT          681 a          894 c          803 g          781 t
ORIGIN      
        1 ttaattacac cacttttccc gtcgccacgt aaaatcatat tcgttataca aagagcggcg
       61 gtagcaggct ccgattcaat gccaaatagg ttattcttga ctttcctaac accctcctcg
      121 taaggaatgc tctttttctt gatcattcgg tttagagcac ctataagaaa accgccggtc
      181 ccacaggcgg gatcaaaaac tatgtcttgg ggcgtaacat ctacaagttc acacataaat
      241 gatataatat gacgaggagt gaagtactga cctatggtgt tccctccggt atagcgaaag
      301 aaagtttcat acaactggcc gagataatca tgttcttgca taaaggagcg tatattgagc
      361 ttcttgagaa tgtcaattat ctcccatgca cgtgcagcaa gtgcatcgtt ttcgtcatca
      421 actcggagac tgtgagctag ttcaggcttc ccggctttct gaagtgcctg ttcagcattg
      481 gcgttgactt gagttaatac aacttcctta tcagttgaaa cattgccttt ccagagagcg
      541 agcataaatg ttgcaacgta gatcgggcga tattcgtctt taattttgca ttcacgaaga
      601 atctcattaa gcttgttagc ttgagaagct agtacttcct ctgacggccg ttcaggctca
      661 acctctattc tgtttctact cgcaaggatt gagattgtct gtctcggaga aggaatccag
      721 tcaatcggtg aaccatgcag tgtcaaatcc ttccagttac cgttcactct ccgctgaact
      781 tttacggaac agatttctct ttcagcacca gcaacggcaa cggaaagaac ctcaatacca
      841 gaatcaaaac atgcgtttcc gtagtgatta gcgtctttaa cggcatcttt gaagagtgct
      901 aacgtggctt tagtatcgat gacgatgagc ggtcttagac tttcgggcga ttcaacgagt
      961 aagaagtcgg gtttgccaga accaggacca gtcttagatt tcccttcaaa caatccctca
     1021 agtgtagaat agttcttata ctcgttttct tccaaaactt gtcccccact tgaaacagga
     1081 cgagggtccc aaccacgaaa taggagaagc tccctagtta cttccctagc tctttgctca
     1141 gagaggcgtt ttggagacat tttagaaata tcttgttgaa atttctatcg aggggccgca
     1201 ctcagtataa ccgtcatcta cgtaccgaga gttaacccga acatgttttg caaacgttct
     1261 acacccgcac cgcggctctt tttttgctct cttgtcgctg tggcatgggc ttctccttat
     1321 ctcgaaccac agtcaaccct accagccggt cctcctgcca ctcataccgg agtcggcccc
     1381 gggccttcgt atcgcaccgc gccatcacat aggccgccaa aagcgggagc gacaccgagg
     1441 gttccacaaa ggccatcgca aagtcggcgt ccaagtcaat tttcccccac gactgcgcct
     1501 cgcccagagt cgagccggag agcccgcccc agtgggggga gtcggtggtc acctgcaggg
     1561 cgtaggcgtg cccgcgctgc cggccgaaaa tgtaccccat caccaccgag tcgttaatgt
     1621 agttcttggg caccccgccc gccacgtaaa tcgccgccgt ctcggctgac ttgacgacga
     1681 tctgggtcag ttcgtagttg tcccggatcg aatcgagcgc catccccggc tttttctttt
     1741 gggccatccg gtggtagtgc tcggtcaagc cgatgccgat ggaggagtcg tttatggccg
     1801 gagcgaagat ggggacgccg agacgatggc actgcgccac gattgattcg tcatcgtcca
     1861 acttactgcc taagtggtcg aggtagaaac ggctggagac gcgcgcggga gccagttcat
     1921 ccgccttctt cccgcaccag aggtcggtct cccagaattt ctcttcgtcc acgtaggtat
     1981 cgtaaatccg gtttatgtat aactcgcgga gaactttgtc gtcggcgttg ggactgcccc
     2041 ggaaatgctt gaacccccgc gcctggtaaa tatcctggta gagaatcgcg ccggtggaga
     2101 cgatgacatc gacatacccg cccactacca ggtcgcgaat cacttttcgc aatcctgccg
     2161 ctatgagagg tcccgcaaga cctaagagaa tcgtggggcg gcgggggtcg tctaacatct
     2221 tctgccagac gcgggcgcac tggccgacgt tgcgcgcctg caccgacatc ttggccatct
     2281 gatccaccaa gtcagcgacg gagatattag aatccagccg cgccggctca atcggcaggg
     2341 tgaggatttt attctttttc tgccgcttat ccgcaggcaa agatggcagg tacgttgcag
     2401 cggggttatt gttctgtgtc attcctccat ccctcccgtg cctcacttcg gcttccgctc
     2461 aaagacctgg tcgatgatcc cgtactcctt cgattcctcc gccgacatga agtagttccg
     2521 gtcggtgtct ttttcgattt tttccagcgc ctggccggtg tgtttggcta aaatttcatt
     2581 cagccgcgag cgggtcagca aaagttcttt ggcgtggatc tggatgtcgg tcacctgtcc
     2641 ctgcaccccg ccccacggct ggtgaatcat aacccgcgag tggggcagtg ccgcccgctt
     2701 ccctttggtg ccggcggcca acaaaagcgc gcccatcgag gccgccatgc cgacacaggt
     2761 ggtcgccacc ggcggcttga cgaactgcat cgtgtcgtag atggcgagcc ccgcggagac
     2821 gatcccgccc ggcgtgttga tgtacatctg gatgtctttc tccgggtctt cggcttccag
     2881 aaagagcagc tgcgcgatca ccaaattggc gatcgtgtcg tccaccggcg tgccgatgaa
     2941 aataatccgg tctttgagta gccgggagta gatgtcgtag gcccgctcgc cccgcccggt
     3001 ctgctccacc accatcggaa tcaaggtcat ctgtctcctc tcatcgtcgt catcctctta
     3061 acgctcacgc gctgttcggt atcgttcatt gccgcaaacg gggtgactgc gccgggctcg
     3121 atgcccagtg tcgcgtgcat gagctcggcc gagccgaag
//
LOCUS       DBNGZD010000124        13695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1341005, whole genome shotgun sequence.
ACCESSION   DBNGZD010000124 DBNGZD010000000
VERSION     DBNGZD010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 13695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13695
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1341005"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="AC16GM_03385"
     CDS             <1..659
                     /locus_tag="AC16GM_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2922751.1"
                     /translation="KHVPVRFNVVQGPLLDVSPTNLFFTGQVGDPDPAAQTVRIDNTG
                     AGTLNWQAAPTQPWVSITPNSGTAPPAQNASVGVHPAGLIPNIYADTVTVSGNAPNSP
                     KQVIVFLQLNGPTAVREISESQLPRAYTLNQNFPNPFNPSTRIRFALPKEGKVTLEVY
                     SILGSKVATLVDEFLSAGTKEAVWNGTDDAGQTQASGIYFYRLKAGDFTDVKKMLLTK
                     "
     gene            817..2031
                     /locus_tag="AC16GM_03390"
     CDS             817..2031
                     /locus_tag="AC16GM_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922752.1"
                     /translation="MKIKFCLLSVVAVLGLFASGCEKKVNRIQGPAPTGVLRPPSGAV
                     IPSPLLTVSFGGTSRELWPFTGSDFSGAPRDPVNLIFVGQSDPRIIRAALLSLNGDRT
                     AFGFPNVFPFNCTWTDAVGDVQTGYATASGWVGSAIQLACGTYGPLRFHLRLFNVGDW
                     TLGGAHFEVLIPGTTEHQVLSWELAEQLVVVDFLRSGLLDPTLPLLPTGVINPSPYRE
                     IPAVIYNGLPADLKALIGGPPGNVSDPVPLTTDGKATILNIAQSVNGLPVVAKQDFVV
                     QFDQVIPKPFCASGPFDFVLVQGPVNFSQKIVMTPAGNFLSRFLAQGHLDVTPVNPLT
                     DPPTPLGETYRALVNQDNRGIITDNVTLVSSFQMQIEIPPTGPFRGRLVVKLKVGPGG
                     SNQFSLSVNCLP"
     gene            complement(2116..2532)
                     /locus_tag="AC16GM_03395"
     CDS             complement(2116..2532)
                     /locus_tag="AC16GM_03395"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR2922753.1"
                     /translation="MRILFLCTGNSARSQMAEGFGRKLFPPEVEIWSAGVEPRKLNSL
                     AVQVMAERGINISRQFSKGVDAVPPPVDLVVTLCGDAAERCPSFASSPRHLHWELPDP
                     ALPQKDKAVPLALFREVRDRIESHVQSLAKELAVRR"
     gene            complement(2581..2665)
                     /locus_tag="AC16GM_03400"
     tRNA            complement(2581..2665)
                     /locus_tag="AC16GM_03400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2630..2632),aa:Leu,seq:taa)
     gene            complement(2967..4220)
                     /gene="nrfD"
                     /locus_tag="AC16GM_03405"
     CDS             complement(2967..4220)
                     /gene="nrfD"
                     /locus_tag="AC16GM_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR2922754.1"
                     /translation="MEPFLQSLPGGTGGISGFVFPNEFELHWGILIVVYPYLTGLVAG
                     AFILASLERVFKVQALEPIYRLSLLTALSFMIVAPLPLLAHLGHPERALEMFLTPHTS
                     SAMAMFGFVYAWYLAVVLLLEIWFDYRRDIVNWSKQKKGPAKVIYRILTLGVSDVSEG
                     AVRFDHKIGKIITVIGIPSAFLLHGYVGFIFGSVKANPWWSSVLMPIVFLFSAIVSGI
                     ALVILVYMVTAMVRWKTINMVCLDKLAELLLYAIIVDFSLELLDFIHRLYESEESINI
                     LSQMISSRLFISLIITQITIGTIIPVISIVMVRTRTVHVEMRRILYFIAAILVQVGIF
                     STRWNVVIGGQLFSKSLRGLTVYKLDLFGPEGVLMAGALLILPFIIIYVLTKLLPPWP
                     QGEQDNISGDEAGRTDAAIVLVKAS"
     gene            complement(4223..4993)
                     /locus_tag="AC16GM_03410"
     CDS             complement(4223..4993)
                     /locus_tag="AC16GM_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2922755.1"
                     /translation="MFTVPKMPRREFLTTFAQKVSGLAGSMVALGSLTALAEEKNDKY
                     NPSEHKYAMAIDIQKCIGCGRCVEACKKENNVPLDDAHFRTWVERYVIAEDGETYVDS
                     PSGGMYGFPDLKEDMKVFRTFFVPKLCNHCENPPCVQVCPVGATYISPDGVVLVDEKY
                     CVGCRYCIQACPYGARYFHPVKKVAEKCTFCYHRITKGLLPACVEVCPTQARIFGDLK
                     KTSSPLRRFFHFMKISVLKPWMNTEPKVYYGGLDGEVK"
     gene            complement(6175..7371)
                     /locus_tag="AC16GM_03415"
     CDS             complement(6175..7371)
                     /locus_tag="AC16GM_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2922756.1"
                     /translation="MSWAGWVFDFYDLVLYTFLLIPIGKELNLTNVELSYVLGASLAA
                     TAVGGVIFGVLSDRFGRKRVLQWTILTYSVGTFLSGLASNLPLLISFRIFTGLGVGGE
                     WATGQTYVGETFPPNVRGRYGAFMQTGAPIGIALASMIGGFLAPAIGWRVCFFLSGLP
                     ALLVVVVRRSLSESDLWLARKQQMEESGERSKYRHFSKFLTLFSKPHRKLFLLALILA
                     IFDMSAYWFTYSWLPGYLHQQRQFSLAKSAAWILVTQCGGFLGYFTFGFVADRLGRRP
                     AYSIYSFLMAAGLVMITLLWETIAVYPAVILACMFLVGFGTGMFGGYGPLFSELFPTE
                     IRNTAMGSAFNLARGVQFFTPVIIAKIASLYGLGGGISLAAFFALLTGIWIWTFPETK
                     GKKLSA"
     gene            complement(8081..9154)
                     /locus_tag="AC16GM_03420"
     CDS             complement(8081..9154)
                     /locus_tag="AC16GM_03420"
                     /inference="COORDINATES: protein motif:HMM:NF021235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2922757.1"
                     /translation="MKRLVAGVAGLCLFGLVVVAWAGDYHYGTTLICSDCHVMHFSQT
                     HGYNNDGGGGIDPPLGTQPEPFLLRDEVNKLCLSCHDGEASTPDVYGNNTLVNTRQAG
                     ALNKTTDGGPYYPANGHTLYSKAIAPGGTWAPDTSEGLNCVNCHHQHGFPGGGFSDPG
                     GTGTYRNLMARPGNAPTNRWITYRVGVNDLTKDVFERATGGVATHYAVSNIDFNEPDQ
                     TKSAYGAWCQGCHTNFHGSSADANMRDAGHAAGTGWLRHPTADANIGALTGGHSSLAV
                     LDSNLYRTQVMSASGNWGTQGSTWPGSPTDLTPSCFSCHKGHGNKNAFGLIYALGNAP
                     LGEEGDGTQAKNLCKQCHVQGAD"
     gene            complement(9630..10586)
                     /locus_tag="AC16GM_03425"
     CDS             complement(9630..10586)
                     /locus_tag="AC16GM_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR2922758.1"
                     /translation="MKRVRRAPGFLVLLVTGPLLAQGPQLLVTIKSLVTGYSSALRFS
                     NPQGIFFDRKFKELYVCDAGNHQVVIFDSTGYPIFRFKHWVEQEGKRILGEPRSMVVN
                     AEGDIFLTDNLADYIDVLDHRGESEDQIDLAKLLHLSTAKPDYLALDGEEHLYVGYRG
                     EKSEVAVLDRGYQVIRQLKNQPDNHQDFESISGLWVDAGGKIYVTDVKAEPCVKVFSN
                     TGVYLFGFGRHEVGWGDFSLPAGVATTTDGTIWIVDTFQQVVKAFNSKGEFIQYIGGY
                     GRHPGDMKYPDAIAGDGDQTIFVLERVGERYQEFVVQTQVRQ"
     gene            complement(10676..11239)
                     /locus_tag="AC16GM_03430"
     CDS             complement(10676..11239)
                     /locus_tag="AC16GM_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HIR2922759.1"
                     /translation="MNRSWAFLFPLFCLVGCSHTPRIYLTPQPNVSGDRTVAVLPFEN
                     LSGEEGAGEKVQKIFLVELLKLRKFSVFEPGEVAQALKELRIRATDKLSQEQASQLKE
                     KLGVDWICLGSVLEFAGQRQGTAEVPVVSLTARILDAASGKIIWAAFESRQGDDAEFL
                     FGVGKINSASELAKKIAEKMVVTLNRR"
     gene            complement(11241..11822)
                     /locus_tag="AC16GM_03435"
     CDS             complement(11241..11822)
                     /locus_tag="AC16GM_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922760.1"
                     /translation="MRKYLGLVVSGAVLWAGCGGSRSFVHPQTDIGFYKKLGVVTFRN
                     LTGDRFAGQKMTSSFVTELLLSKKFDVVEPGEFERIVADVLKGTAGSEEQELNAEQLK
                     AIGEKAGVQGVIEGEVREFQMTRIGSEDFPLISLAIKLVDAPSGQVIWMSSYTAKGGP
                     KFPIFSFGETRTLGELAQKVCRKMVQQFIAQVF"
     gene            complement(11837..13453)
                     /locus_tag="AC16GM_03440"
     CDS             complement(11837..13453)
                     /locus_tag="AC16GM_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922761.1"
                     /translation="MAPVGKTFFLIVVTFLAGRNVSFAYPKIYGNYQFNFQRQLLPLG
                     SNNLYNQILYFNLQDYLMVKNRFLFTAYVVRNEYVSNSKRVDFRPRLDFDLTGTPYKL
                     FFSYLPYALPEAGGVKSVYKQYQGLASLHPGNVPDLRFNWNRTDLHDNQPVRRSDASF
                     RTWSVGSAWTKNFFNAALFYTRQENADRLLQEKTLLANAFNVSGGLNLATPGAGYLSS
                     TYNYSHNEREDRGVKTSTANAHSLTTLYATPLAKNLSLSSNYSGRFSFIRQLLPKQKF
                     QDQTLNTSLSFTPLSRMDLSVLRGDMQSLAPGSRTSQDYWAGTANYAFPVWKAVASKI
                     SYSKTWFVQSPQGQFFSDVYYLSLAAPVYPGLQVRADLSVNFRSGALFVPSRYQTSRF
                     LDIRSQPWEKVQLNYYYQSSVAAPNLTFTQVAAGSHNFALNYFPVPTWSATLNYIERF
                     ASQAVGKQRVFAGQIYHTFREQYTINVNYNLSRFSMPLPGQPRQSDNLSSQLVLFLER
                     RTVLTFSYAASNLTVGTFSSTLGATVNYQF"
     gene            complement(13453..>13695)
                     /locus_tag="AC16GM_03445"
     CDS             complement(13453..>13695)
                     /locus_tag="AC16GM_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922762.1"
                     /translation="GCRGCHAGIYENDANLACDNGSARGTIHGGNFTWPICSKSPGSV
                     SKRFMVGGYLSGWQEGTVNNATCWGGGCHHSNGVRY"
BASE COUNT         3218 a         3765 c         3633 g         3079 t
ORIGIN      
        1 ccaaacacgt gccggtgcgc ttcaatgtcg ttcagggacc gcttctggac gtctcgccca
       61 ccaatctgtt tttcaccgga caggtcggcg accccgatcc cgcggcgcaa accgtccgga
      121 tcgataatac gggcgctgga actttgaact ggcaagccgc tcctactcag ccctgggttt
      181 cgatcacgcc gaattccggc acggccccgc cggctcagaa cgccagcgtg ggggtccacc
      241 cagcgggcct gattcccaac atctacgccg acacggtcac ggtgagcggc aatgcgccga
      301 actctcccaa gcaggtcatc gtcttcctgc aactgaacgg gcctaccgcc gtgcgcgaaa
      361 tatccgaaag ccagctcccc cgggcttaca cgctcaacca gaatttcccc aaccccttca
      421 acccgtcgac gcggattcgc tttgccctcc ccaaggaagg caaagttacg ctggaagtct
      481 atagcatcct gggctcaaaa gtcgccaccc tggtagatga attcctctcc gcggggacga
      541 aagaagcggt ctggaacggt actgacgacg cgggacagac gcaggcctcc ggcatttact
      601 tctaccggct caaggcgggc gatttcaccg acgtgaagaa gatgcttttg acgaagtgac
      661 ggttgcggaa aatgtaacta ctgccgccac ggaataggcg gcgcaaactt tggctgtctt
      721 ctccagggag gtggtgtctt acaagagtaa accgagaacc ggaatatcct gcgagtgttt
      781 ttgacccttc tcttttaagt gaaaggggga atatccttga aaattaaatt ctgcctcctc
      841 agcgtcgtgg ccgttttagg actttttgct tctggctgcg aaaagaaagt aaatcgtatc
      901 cagggaccgg ctcccactgg tgtgctgcgc ccgccttcgg gagcagtcat tccgtctccg
      961 ctcttgacgg tttcttttgg aggaacgtcc cgggaattgt ggccctttac gggaagtgat
     1021 ttctccggtg cgccccggga tccggtcaat ctcattttcg tcggccaatc ggatcccagg
     1081 atcatccggg cggccctctt atccctgaat ggcgaccgga ccgccttcgg ctttccgaat
     1141 gtctttccgt tcaactgtac ctggaccgat gcggtgggag acgttcagac cggctatgcc
     1201 actgcctccg gttgggtggg cagcgccatt caactggcct gcggtaccta tggtccgctc
     1261 cggtttcacc tgaggttgtt taatgtcggc gattggactt tgggcggcgc ccatttcgaa
     1321 gtgctcattc cgggcacgac ggaacaccag gtgctgagtt gggaactggc ggagcaattg
     1381 gtcgtcgttg atttcctgcg cagcggtctt ctggatccta cccttcccct cctgccgacc
     1441 ggagtaatca acccttcccc gtaccgcgaa attccagcgg taatctacaa cgggctgccg
     1501 gctgacctga aagcgctcat cggagggccg cccggtaatg tgagcgaccc ggtgcccctc
     1561 accactgacg ggaaagccac cattctcaat atcgctcaaa gcgtgaatgg cttacccgtc
     1621 gtcgccaagc aggactttgt ggttcagttt gaccaggtga tccccaagcc gttctgcgcc
     1681 tccggtccct ttgattttgt gctggtgcaa gggccggtga atttcagcca gaaaattgta
     1741 atgacccccg ctggaaattt cctgagccgg tttcttgccc agggtcattt ggacgtgacg
     1801 ccggtaaatc cgctcaccga cccgccaaca ccgctcggtg agacgtatcg ggcgttagtc
     1861 aaccaggaca acagaggaat aataaccgac aacgtcaccc tggtctccag tttccagatg
     1921 cagattgaga ttcctccgac cggccccttc cgcggccgac tggtggtcaa gctaaaggtt
     1981 ggtcccggcg gctccaacca gttttccctg tctgttaact gcctgccgta gggattagcg
     2041 cagaacggaa aacccacgca cccgcgtggg ttttttattt ctgggcgaag tttttctgcc
     2101 aatcgagcgc cataattacc gtctgacggc caattcttta gccagcgatt ggacgtggga
     2161 ctcgatcctg tcgcgcactt cgcgaaaaag tgcgagcggg actgctttgt ctttctgcgg
     2221 cagggccgga tcgggcagct cccagtgcag gtgtcgtggg gaacttgcaa aagatggaca
     2281 gcgctctgcc gcatctccgc acagagtaac cactaaatcc actggcggcg gaacagcatc
     2341 aactcctttc gagaactgcc gggaaatgtt gattcccctc tcggccatca cctgcactgc
     2401 cagcgaattg agttttcgag gttccacccc ggcgctccag atttcaactt ccggcgggaa
     2461 gagctttcgg ccaaaacctt cggccatctg gctgcgggcc gaattgccgg tgcagaggaa
     2521 taaaattctc atgcgaaact atcttcgcat tctcagggga acatcggtca aagcgaaaaa
     2581 tgccggaggc cggactcgaa ccggcacccc gaagtatcgg ggagggattt taagtccctt
     2641 gcgtctacca ttccgccact ccggcatttt cttctcaggc aataccttac ttcacttttc
     2701 caagatagta aattattggg cttgtgacca aattgagtcc ctatgctgcc acctttcgat
     2761 ggcgaaagaa tacttcatcg gaaaaggatt cgagaaaaag aaacctcttc cgccgaatga
     2821 acgttttgcg aaccgcaaac tgaagccctt tgaaaaagtg tcatagaatt gggggtaggt
     2881 acacacccga aattcttgca ccgagagtag tcgaggggtg ggcacccggc cgaagactcc
     2941 ggatatttca acgcagagat ttcaggtcag gatgctttca cgaggacgat ggcagcatct
     3001 gttctgccgg cttcatcacc ggaaatatta tcttgttcgc cttggggcca gggcggcaac
     3061 aattttgtca agacatatat gattataaac ggcaaaatca acaaagcgcc ggccatcaaa
     3121 accccttcgg ggccgaacaa gtccagctta taaacggtca gcccgcgcaa actcttcgag
     3181 aaaagctgcc cgccaattac cacattccaa cgagtgctga aaatgccgac ttgtaccaga
     3241 atggcggcaa tgaaatacaa aatccgccgc atttcgacat gaaccgttct cgtccggacc
     3301 atgacgatcg atatcacggg aattatggtt ccgatagtaa tctgggtaat aatcagactg
     3361 atgaacaatc ggctggaaat catttgcgaa agaatattga ttgattcttc tgattcatag
     3421 agtcgatgga tgaagtccag gagctccagt gaaaaatcca ctataatggc atagagcaaa
     3481 agttccgcaa gtttatccag acagaccatg ttgattgttt tccagcgaac cattgctgtc
     3541 accatataaa ccagaataac caaagcaatt ccggaaacta tagcggaaaa gagaaacacg
     3601 atgggcatca ggacgctgct ccaccagggg ttggctttga cggacccgaa gatgaagcca
     3661 acatagccat gcagcagaaa tgccgaagga atacctatga ccgtaataat ctttccaatt
     3721 ttgtggtcaa acctgaccgc tccttcagat acatcgctga ctccaagtgt taaaatgcga
     3781 taaataacct tggccggccc ttttttttgt ttggaccaat taacaatgtc gcggcgatag
     3841 tcgaaccata tctccaaaag taataccact gccaaatacc aggcatagac aaaaccgaac
     3901 atggccatgg ccgacgaagt gtgcggggtt aaaaacattt ccagcgcgcg ctccgggtgc
     3961 cccaggtgag ccaaaagcgg cagcggcgct acaatcataa aagaaagggc cgtcaataag
     4021 gaaagccgat aaattggctc aagggcctgg actttgaaga cgcgctccag cgaggccagt
     4081 ataaaggcac cggccaccag tccggtcagg taaggataga ctacaatcaa gatcccccag
     4141 tgcaattcaa attcattagg gaacacaaag cccgagatac ctccggttcc acccggcagt
     4201 gattgcagaa aaggttccat gattatttca cttctccgtc gaggccgcca taatacactt
     4261 tgggttcggt attcatccac ggttttaaga ccgagatctt cataaaatga aagaatcttc
     4321 taagcgggct gctggttttt ttcagatcgc cgaaaatgcg ggcttgagtc gggcaaactt
     4381 ccacacaggc gggcaaaagc cctttggtga tgcgatgata gcaaaatgtg cacttctcgg
     4441 ctaccttctt taccgggtgg aaataacgag ctccataagg acaggcctgg atgcagtaac
     4501 gacagcccac gcagtatttt tcatcgacca gtaccacccc gtcggggctg atataggtgg
     4561 cgcccaccgg acaaacctga acgcaaggcg gattctcaca atggttgcaa agcttgggta
     4621 caaaaaacgt gcgaaaaacc ttcatatctt ctttcaggtc cggaaagcca tacatgccac
     4681 cgctcggact gtccacatag gtttccccgt cttcggctat gacataccgc tctacccagg
     4741 tacggaagtg cgcgtcatcg agaggaacat tgttttcttt cttgcaggcc tcaacacagc
     4801 ggccgcagcc aatgcacttc tgaatgtcga tggccatcgc atacttatgt tctgatggat
     4861 tgtatttatc gtttttctct tccgccaggg ccgtcaaact gcccagcgcc accattgacc
     4921 cggccagtcc ggaaactttc tgggcaaagg tcgtaagaaa ttctcgcctt ggcattttgg
     4981 gcacagtaaa cattactact tcccctccgg gtcatgggga taatgacaat cccagcacac
     5041 gtgccgttcg ctgtgggact ctatatcgac tctgggaatc tcgcttgagc cggtcgcatt
     5101 cttagcatgg cagttggcac aggtctgaat attggttggc ttctcggcat ggatgaggcg
     5161 ggggttgaca ttatgacctt tgcgcacatt gtggcactgc gtacagggca gattggtgtg
     5221 tactgataca agcttttggt tggcaatact cctgtggcag gcactgcatt cctcgggcgt
     5281 gtgaggaggc ctgggatcat ggggattatg acaggacata caggctttcc caggattgtg
     5341 ctgattcttt agaatttgtg gaaaaccggt cggacgggaa ggattatatc catggcaaac
     5401 gggacagaat tctcgccctt tgggcttgaa aggcagattt tcatcgggag cgttagcgtg
     5461 gtcagcacac ggcccatgac aggcctcgca cggtacttta cgatgaaatg aaccggcttt
     5521 aagttctttt atttcagaat ggcagtccgc acagacctct gctccggcat atttgatttt
     5581 gatctcagaa gcctccttta gcgcgtcgcc tctataaaag cccatcttgc cgaaagatgg
     5641 cgggacgaac atttgacggg cggtaatgaa agccgcgatg accagcgcga acagaacgaa
     5701 aagtgggatt aactgtctcg gaattctgga cataaccttc gcctaaacac ccttgggtaa
     5761 aaacctacgt tttctacttt acgatacaat actattatta taatatatcg gctgaaaaat
     5821 gaaacaccag ccccgccttg tcaagtgggt aggttgagtg gagttgctga atagctcagc
     5881 cactttccaa aaccatccct ttatctcgac ccaatacggt gcggcagatt cgagagtaaa
     5941 gcagtttgcc ccaactcgct gtctattaac ggcttatgcc acttccccgg ttcaactcgt
     6001 tgggttttgt tcggaagaag ttctgcctat gtagaagtga acgtccccga gcttattgat
     6061 acgcgcacca ttcggttcga ggtttgttca taggaggtgc ccgggtgggg caatatgcga
     6121 accgttaccc gcaattcttt gaaaaagagc cttatctttg ggattcttat atcgttaagc
     6181 tgaaagcttt tttcccttcg tctccggaaa ggtccaaatc cagataccgg tcaaaagggc
     6241 aaaaaaggca gccagagaaa ttccgcctcc cagcccataa agagaggcga ttttggcaat
     6301 aatcacgggc gtgaaaaact gcactccacg ggccaggttg aaagccgagc ccatggcggt
     6361 attgcggatt tcagtcggga aaagctccga aaaaagcggg ccatagccgc cgaacatccc
     6421 ggtgccgaag ccgaccaaga acatacaagc caaaatcacc gccggataga cagcgattgt
     6481 ttcccacaga agggtaatca tcaccagtcc ggccgccata aggaaagagt aaatcgagta
     6541 agccgggcgg cggcccaagc ggtcggccac aaagccgaac gtaaaatacc ccagaaatcc
     6601 gccgcattgg gtcaccaaaa tccaagcagc tgacttggcc aaggaaaatt gcctttgctg
     6661 gtgcaagtaa cccggcaacc aggagtacgt gaaccaataa gcagacatat caaaaatcgc
     6721 caatataagg gccaaaagga aaagcttacg gtgcggtttt gaaaagagcg tcaagaattt
     6781 tgaaaagtgt ctgtattttg accgttcacc tgattcttcc atctgctgtt ttcttgccag
     6841 ccaaaggtct gactcagaca gggaccggcg aaccacaact accaatagcg ccggcagtcc
     6901 agagagaaaa aagcagaccc gccaaccgat ggccggggcc aagaatcctc ctatcatcga
     6961 agcgagggcg atgccaatcg gcgcgccggt ctgcataaaa gcaccatatc gcccccggac
     7021 gttgggtgga aaagtttctc ccacgtaggt ttgtccggtg gcccattctc ccccaacgcc
     7081 gaggccggtg aaaattcgaa acgagattaa gagtgggagg ttggaggcca agccggaaag
     7141 aaaggtacca acgctatagg tcaaaatcgt ccactgcaaa accctcttgc ggccaaagcg
     7201 gtcggataat acgccaaaga tgactccgcc tacggctgta gccgccagcg acgcccccag
     7261 aacgtaagac agctcaacat tggtgagatt tagctccttg ccgatcggaa ttaggaggaa
     7321 cgtgtagagt accaaatcgt aaaaatcaaa aacccaccca gcccaactca tcaccaatat
     7381 tcgatagtgt tcacgaatgg gcctgtcgta ttcgtttaga aattgcgctt tcatagcagg
     7441 aaacggcctg gccgttcggt aagcaagtaa gtctgatgac ccacaaccgt caagaaaaaa
     7501 tcccgaaggc gccccccggc cctccgaagg acactttcct gactcaaaac tgtgttacct
     7561 cgtgactctc aaaataaata ccttttctcc caaggcagga ttcgaaccag tgttttttgg
     7621 gcgaggggag taaaaacgtc agaggaaatg aaacgttctc ttgtgaccaa tttgtgacta
     7681 tgaataatcc caaaatagtc caatacaatc cccgataatc ctcgcatgcg cttccactat
     7741 ccacttggcg ctcaaaatat tgtgctgatt tccaacgagt tgcaataagg gtgcaaaggc
     7801 tttttaagtc ccctgcgtct acccttccgc ctctccggca accgttcttg gaacgataac
     7861 ttatcttctg aagcgtccga cttcaagcgc cccccggctg cccaatagag cttcccctcc
     7921 tgtccccttt ccacccgcat gtcaggtgca aggagcagaa agaaacccgg gaaaaattgg
     7981 ctgaagtaat ttacccagac aagatcagaa atttccactt ccaaaactta aaggcgctcg
     8041 tttgcgagcg cctctaaggg taaaccaacc tgcgtcgact tcagtccgcg ccctgcacgt
     8101 ggcactgctt gcacaggttc ttggcctgcg tcccatctcc ttcttcaccc aggggggcgt
     8161 tccccaaagc gtaaatcagt ccgaacgcat tcttattgcc gtgccccttg tggcaggaga
     8221 agcaggaagg ggtcaggtca gtgggtgaac caggccacgt agacccctga gttccccagt
     8281 tgccgctggc gctcatcact tgcgtgcggt agaggttgct gtccaagacc gccaagctgg
     8341 agtgcccgcc ggtgagggca ccgatgttgg cgtctgcggt cgggtgccgc aaccagccgg
     8401 ttcccgcggc gtgtccggca tcccgcatat tggcgtcagc cgaagaaccg tggaaattgg
     8461 tgtggcaacc ctggcaccaa gcaccgtacg ccgacttcgt ctggtccggc tcattgaagt
     8521 cgatgttgga aacagcgtaa tgcgtggcga ccccaccggt agctctctca aatacgtctt
     8581 tggtcaggtc gttcactccg acccgatagg tgatccaccg gttggtgggg gcattccctg
     8641 gccgcgccat gagattccgg taggttcctg ttcccccggg atccgaaaac ccgcctcccg
     8701 ggaatccgtg ctggtggtgg cagttcacac agtttaagcc ctcgctggta tccggcgccc
     8761 aggtgccacc gggtgcgatg gctttggagt agagcgtgtg tccgtttgcc ggatagtacg
     8821 gcccgccatc cgtggttttg ttcaacgctc ccgcctggcg cgtgttgacc agcgtgttgt
     8881 tcccgtacac gtcgggggta gaagcctccc cgtcatggca gctcaggcag agtttgttga
     8941 cctcatctct caggaggaat ggctcaggtt gggttcccag cgggggatca ataccacctc
     9001 cgccgtcatt gttgtaccca tgggtctgag agaagtgcat gacgtggcag tccgagcaga
     9061 tcaaggttgt gccgtaatgg taatccccgg cccacgctac aaccactagt ccgaacaagc
     9121 acagacccgc gactcctgct actaaccttt tcatttcttt ttcacctcct tcgctctgtt
     9181 accgtttcgc tgagaattct tcactctcat tcgttccact cgcacaaccc agtctgccga
     9241 gagctcttga gtgtcgctcc tcacctcccc tgcagttgcc gaacattgtg aatactttca
     9301 cacactctcg tatgacctac gtcatccctc atttcgccgt tggtcgctta atagagaact
     9361 cagagcgtcg tattgtgaac attctcacaa gcactattat atcacctctc cggaatttgt
     9421 caagagaaat ttacatcttt ttatcttgta tctaagtcgt tgggggtgaa gtaactacgc
     9481 gagaaaacaa ctccttatat tttcaggcca aaaattcgag caacgctcac tcgagaggta
     9541 aagaggcaat cttgggagtc gattgaggat agaatcagtc tttttccctt ttcgaacgaa
     9601 aacctcagac aaagagcccg gactaaacgt cactggcgga cctgagtttg gaccacaaat
     9661 tcctggtatc tttctcctac gcgctccagg acgaaaatag tttgatcgcc atctccggcg
     9721 attgcgtccg gatatttcat atctcccgga tgccggccat agccgccgat gtactggatg
     9781 aattctcctt tggaattaaa tgctttgacc acctgctgga acgtgtccac aatccagatc
     9841 gtcccgtctg tggttgtggc cactcctgcc gggagagaaa agtctcccca accgacctca
     9901 tgccggccaa aaccgaaaag gtacacgccg gtgttggaga acactttcac gcagggttcc
     9961 gccttcacgt cggtgacgta gattttccct ccggcgtcga cccagagacc ggaaatactc
    10021 tcaaagtcct ggtggttgtc cggttgattc tttaactgtc ggatcacctg atacccgcga
    10081 tccagaacgg ccacttcgct tttctcgccg cggtaaccga cgtagagatg ctcctcgcca
    10141 tccagggcga ggtaatcggg cttggccgtg gagagatgca atagcttcgc caggtcgatc
    10201 tggtcttcgc tttcgccccg gtggtctaaa acgtcaatgt agtcggctaa gttatcggtc
    10261 aagaagatgt ccccctccgc attgaccacc atacttctgg gctcgcccag gattctcttc
    10321 ccttcttgct cgacccagtg cttgaagcgg aaaatgggat agccggtgga atcaaaaatg
    10381 accacctggt gattgccggc gtcacagacg tagagctctt tgaattttct gtcaaaaaaa
    10441 atcccctggg ggttggaaaa acggagggcg gaactgtagc ccgtgacgag cgatttgatg
    10501 gtaaccaaaa gctggggacc ctgtgccaga aggggacctg tcaccaggag gacaaggaaa
    10561 cccggagcgc gccgaactct tttcatacgg actatttcac acctcgcccg gtctgcccgc
    10621 gaggttgcct tctttgagta tacggggaag tccggtgagc tgcttactgc cgctgctacc
    10681 gccggttgag ggtgaccacc attttttcgg ctattttttt ggccagttct gaagcggagt
    10741 tgattttccc cacgccgaag aggaattccg catcatcgcc ctgccgggat tcaaaagcgg
    10801 cccagataat cttgccgctg gcggcgtcca ggatccgcgc cgtgagcgaa actaccggaa
    10861 cttctgcggt gccctggcgc tggccggcaa actccaaaac cgaacccagg cagatccagt
    10921 ccacccccaa tttttctttc aactgggagg cctgctcctg agaaagctta tctgtggccc
    10981 ggatgcgcag ttctttcaac gcctgggcca cttcccccgg ttcgaagact gaaaattttc
    11041 tcagcttgag aagttctacc agaaatattt tttggacttt ttccccggcc ccctcttcgc
    11101 cggagagatt ttcaaacggc aggacggcca ctgtgcggtc gccggaaacg ttcggctggg
    11161 gtgtgagata aatccggggg gtgtggctgc agccgaccag gcagaagagc gggaaaagga
    11221 aggcccagct ccggttcatc ttaaaagacc tgcgcgatga actgctggac cattttccga
    11281 cacactttct gcgccagttc ccccaaagtg cgggtttcgc cgaaagagaa aatggggaat
    11341 ttcggcccgc ccttggcggt gtacgaagac atccagatca cctggccgga aggggcatcc
    11401 accagtttga tggcgagcga tattaagggg aaatcttctg acccgatgcg cgtcatctgg
    11461 aattctctga cctccccttc gatgacgccc tgcacgcccg ctttttcccc gatcgctttc
    11521 agctgctcgg cgttgagctc ttgctcttcg gatcctgcgg tgcctttcag gacatccgcg
    11581 acgattcttt cgaattcccc cggctccacc acgtcaaatt tcttggagag gagcagctcg
    11641 gtgacaaagg aggaggtcat tttctggccg gcaaagcggt cgccggtgag gttccgaaac
    11701 gtgaccacac ccagcttctt atagaaaccg atatctgtct gcggatgcac gaaactgcgc
    11761 gagccgccgc agccagccca cagaaccgcc ccgctcacaa ccagacccag gtattttctc
    11821 atctgctccc ctgccctcag aattggtaat tcacggtggc gcccaacgtg gatgagaagg
    11881 tccccaccgt caaattactc gctgcgtagc taaaggtcaa aactgtcctc cgctctagaa
    11941 acaagaccag ctgggacgac aggttatcac tctgccgggg ctggcccggt aacggcatgg
    12001 aaaaacggct cagattgtag ttgacgttga tggtgtactg ctcacggaag gtatggtaga
    12061 tttgaccggc gaaaacccgc tgttttccca ccgcctggga ggcgaatcgc tcgatgtaat
    12121 tgagcgtggc gctccaggtc gggacgggga agtagttgag ggcgaagttg tggctgccag
    12181 ccgccacctg ggtaaaggtg aggttcggtg ccgcgacgct ggactggtag tagtagttca
    12241 gctgcacctt ttcccagggc tggctgcgga tgtccaggaa acgtgacgtc tggtacctgc
    12301 tcggcacaaa taaggctccg gagcggaaat tgaccgagag atcggctcgg acctgcaggc
    12361 cgggatagac gggcgccgcg agcgaaaggt agtagacgtc cgaaaaaaat tgcccctgcg
    12421 gcgactggac gaaccaggtc ttggaatagg agatcttgct tgcgacggct ttccacaccg
    12481 ggaaagcgta gttggccgtt ccggcccagt agtcctggct cgtgcggctg cccggggcta
    12541 agctctgcat gtcgccccgc agcacggaca aatccattcg ggaaagcggc gtgaaggaga
    12601 gtgacgtatt gagcgtttga tcctgaaatt tctgtttggg tagcagttgg cggatgaatg
    12661 aaaaacgtcc cgaatagttg ctggagagcg agaggttttt cgccagcggg gtggcgtaga
    12721 gggtagtcag cgaatgcgcg ttggcggtcg aggttttcac cccccgatct tcccgctcgt
    12781 tgtgggagta attgtaggtt gaagataagt agccggcgcc gggcgtggcg agattgagtc
    12841 ctccgctgac gttgaatgca ttggccaaga gcgtcttttc ctggagcagg cggtcggcgt
    12901 tctcctggcg ggtatagaac agggcggcgt tgaagaagtt tttcgtccag gcggagccca
    12961 ccgaccaggt gcgaaacgag gcgtcgctac ggcggaccgg ctggttgtcg tgcaggtcgg
    13021 tgcggttcca gttgaaacgg agatcgggaa cgttacccgg gtggaggctg gctaacccct
    13081 ggtactgttt atagactgat ttgaccccgc ccgcctcggg gagggcgtag ggcaggtagc
    13141 tgaagaagag cttgtacggc gtgccggtga gatcaaaatc gagccggggg cggaaatcga
    13201 cccgcttgga attgctcacg tattcgttgc gcacgacata ggcggtaaag aggaaacgat
    13261 tcttgaccat gaggtagtcc tgcaggttga aatagagaat ctggttatag agattattgg
    13321 aaccaagagg caaaagttgc cgctggaaat tgaactggta gttcccgtaa atcttcgggt
    13381 aggcaaaaga gacgttcctc ccagccagga acgtcacgac aatcaagaag aaggttttgc
    13441 ccaccggagc catcagtacc gcactccgtt gctgtggtgg cagccgccgc cccagcaagt
    13501 ggcgttattg accgtcccct cctgccagcc ggagagatag ccgcccacca tgaaccgctt
    13561 gctcacgctg cccggggatt tggagcagat cggccaggtg aaattgccgc cgtgaatcgt
    13621 gccgcgcgcc gagccgttgt cgcaggcaag gttggcgtca ttttcgtaaa tccccgcatg
    13681 gcagccccgg cagcc
//
LOCUS       DBNGZD010000125         4154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_160105, whole genome shotgun sequence.
ACCESSION   DBNGZD010000125 DBNGZD010000000
VERSION     DBNGZD010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_160105"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..916)
                     /locus_tag="AC16GM_03450"
     CDS             complement(50..916)
                     /locus_tag="AC16GM_03450"
                     /inference="COORDINATES: protein motif:HMM:NF041895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HIR2922763.1"
                     /translation="MRKVLLGSICFLALPIILFAYSFGPPPGFTGAPGELSCTFCHIG
                     APLNPGDATLSVIGLPTDYQPGATYPLSATLSDAGHFRWGFQLVVKDTAQLQAGTIVR
                     TDTVNTQIMTSFDGHQYLEQTSTGTFQGSSGPAVWNFSWTAPPQGTGPVTFYAAGNVT
                     NNDLFSSGDSIYTRSFSVPEMTTGVTREEKAGVPSSFTLSQNSPNPFNAATQIVFDLP
                     GGETRPGRLELFNILGQKIKTLIDEPLPGNRYRVEWDGTDEAGRRVASGIYFYRITSD
                     QRQITKRMALLK"
     gene            1116..2195
                     /gene="rlmN"
                     /locus_tag="AC16GM_03455"
     CDS             1116..2195
                     /gene="rlmN"
                     /locus_tag="AC16GM_03455"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR2922764.1"
                     /translation="MKKLQPLNLIGLWLPELEELVERLGERPYRARQLAQWIYKRGMT
                     DFERMTDFAKLFRQRLAEVATCERLVCQSTLESPLDRATKYLFELADGERIESVRIRM
                     NEKITLCLSSQVGCPLACTFCATGDMGFRRNLTAGEIVSQVLQLREDLKEQKINNVVF
                     MGMGEPLLNYEQVVKAIRIMTSELGCELPARRITVSTAGWVPGIYQLAEEKLKVKLAV
                     SLNAPDEERRKKLMPVTRKYSLDELLKAAKHFAFRTRRRVTFEYVLMEGVNDNPADAH
                     LLAKLVRGIPCKINLILYNPNKGQPYIRPALERVERFREILYPRAPAVTLRISKGWDI
                     QAACGQLAADYPLRKRTLRKTVTTV"
     gene            2327..2662
                     /locus_tag="AC16GM_03460"
     CDS             2327..2662
                     /locus_tag="AC16GM_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030077 - plasma membrane
                     light-harvesting complex [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center cytochrome c
                     subunit family protein"
                     /protein_id="HIR2922765.1"
                     /translation="MRKIAAASAGIGLILLVGFGLAQQPPSPGGEKNQPASFKNVQVL
                     KNLTPKEMNLEMVKMSEALGVKCDFCHNLNDWGSDVKKEYQTSRVMLRMVQNINANYL
                     TWDSAPKIA"
     gene            3057..3461
                     /locus_tag="AC16GM_03465"
     CDS             3057..3461
                     /locus_tag="AC16GM_03465"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2922766.1"
                     /translation="MPVATEKKVKTAKILIIDDEPEITEIVQTFLENAGYQVMVENSS
                     VMGIERAKKGLPDLILLDIMMPFMDGYGVCNELKKNPATGNIPVVFLTGKDTRDDSGR
                     SFKVGGDMFIKKPFSCERLLEIVKIVLQSLNK"
     gene            3520..>4154
                     /locus_tag="AC16GM_03470"
     CDS             3520..>4154
                     /locus_tag="AC16GM_03470"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR2922767.1"
                     /translation="MVRPGLLAFLFFCLLSSFFSAVSWAEEYPGPVGYVNDFAGLLAE
                     APAGALEQQLRQFEESTGVEVAVATVATTGGLAVQDYANELFKRWGVGKKGKDNGVLL
                     LVARDDRNVWIEVGYGLEPVLPDALVGQIFLNEIRPRFRAGDFSGGIVAGVNALQAAI
                     EGKYTGQPSKKRPRSVVAYPILLVMLFIGVLVLSSFVSGGRRGGKRSGWIFL"
BASE COUNT          966 a         1121 c         1186 g          881 t
ORIGIN      
        1 gtatacttct ggtgtcaaat cctttataat caccaaaaac attcaacttt cacttcagca
       61 gtgccatgcg cttcgtaatc tgccgctggt cagaggtgat gcgataaaaa taaatcccgc
      121 tggccacccg ccgtcccgct tcatctgtgc catcccactc gacgcgataa cggttgccag
      181 gcagcggttc gtctatgagc gtttttattt tctgccccag aatattgaaa agttccagcc
      241 gccccgggcg agtctcgcca ccgggcaggt caaagacaat ctgggtggcg gcgttgaagg
      301 ggttggggga attttggctt aaagtaaaac ttgaaggaac acccgccttt tcctcccggg
      361 tgacgccggt ggtcatctcc ggtacggaga aagagcgcgt gtaaatagaa tcaccggaag
      421 aaaacaggtc gttattggtg acattgccgg cagcgtagaa ggtcactggt cccgttccct
      481 gtgggggcgc agtccaggag aagttccata ccgccgggcc ggagctgccc tgaaaagtac
      541 cggttgacgt ctgctccagg tattggtgac cgtcgaagga ggtcatgatc tgcgtgttga
      601 cagtatccgt ccggactatg gttcccgcct gtagctgggc ggtatccttt acgactaatt
      661 gaaagcccca ccggaagtgc cctgcgtcgc tcaatgtcgc cgacaacggg taggtcgctc
      721 ccggttggta atcggtcggc agcccgataa cggagagggt tgcatccccc ggattgagcg
      781 gtgctccaat gtggcagaac gtacacgata gttctccagg agcacccgtg aaacccggag
      841 gtggaccaaa tgaataggca aaaagaataa tgggaagcgc gagaaaacaa attgaaccca
      901 gtaagacctt tcgcattacc tcacctctcg attttacgac cttccgaatc ttacgactcg
      961 cgctcaccct tctcacgggg cgacgctttt cgcagggaag agaatcaccg aactattata
     1021 acaaaagaac tgtcaaattg ttgctaatat tttcgccggg gttgcccttt ttcagcattt
     1081 tcgcggtcgt atattacgtt agtatggtct tgactttgaa aaagctacaa ccactgaacc
     1141 tcatcggcct ctggctgccg gaattggagg aattggtcga acgcctgggg gagcgtccct
     1201 accgcgcccg tcagttagcg caatggatct acaagcgcgg gatgaccgac ttcgagcgga
     1261 tgaccgactt cgccaagctg ttccgccagc ggctggccga ggtggcgacg tgcgaacgcc
     1321 tcgtctgcca gagcacgctc gaatcaccgc tcgaccgggc gacgaagtac ctgttcgaac
     1381 tggccgacgg cgaaagaatt gagtcggtca ggatacgcat gaacgagaaa atcacccttt
     1441 gcctctcctc ccaggtcggc tgccctttgg cttgcacttt ctgcgccacc ggcgacatgg
     1501 ggttccggcg caatctgacc gccggcgaga tcgtgagcca ggttttgcaa ctgcgcgagg
     1561 acctgaagga gcagaagatt aataacgtcg tcttcatggg aatgggcgag ccgctcctca
     1621 attacgaaca ggtggtcaag gcgatccgca ttatgacatc ggaattgggc tgtgagctcc
     1681 ccgcacgccg gatcaccgtt tccaccgccg ggtgggtgcc gggtatctac cagttggccg
     1741 aggagaaact gaaagtgaaa ctggccgtct cgctcaacgc tccggacgag gagcggcgga
     1801 agaaactgat gcccgtgacc cgcaagtatt cgttagacga gctgctcaaa gccgccaagc
     1861 acttcgcctt ccgcacccga aggcgcgtca ctttcgagta cgtcttgatg gaaggggtca
     1921 acgacaaccc cgcagacgcc caccttttgg cgaaactcgt ccgggggatt ccctgcaaaa
     1981 tcaacctcat tctttacaac ccgaacaaag ggcaacccta cattcgaccc gccctggagc
     2041 gggtggaacg tttccgtgaa atcctctacc cgcgcgcgcc ggcggtgacg ttgcgtatca
     2101 gcaaggggtg ggatatccag gccgcctgcg gccagttagc agccgattat ccgctgcgca
     2161 aacgaacttt gcgcaaaacc gtaacaacgg tgtgaattcc tcttctcacc tcctcccaat
     2221 cgcaatagtc cgcataacct tggatgaaca accgctgctt tagttcggtt atagagtaaa
     2281 agatgacgag agcacggtcg ccggaccaaa aatggaagga gactccatga ggaagatcgc
     2341 cgcagcaagt gcaggaatcg gtttgattct tttggtcggg ttcgggctgg cacagcagcc
     2401 cccatctccg ggcggagaaa aaaatcagcc ggcgtctttc aaaaacgttc aggttctcaa
     2461 gaacctgacc cccaaagaga tgaacctcga gatggtgaaa atgtcggagg ccttgggggt
     2521 gaagtgcgat ttctgccaca atttgaacga ttggggctcc gacgtcaaaa aggaatatca
     2581 gacgtcccgg gtgatgctcc ggatggtgca gaacatcaac gccaactacc tgacctggga
     2641 tagcgccccc aaaatcgcct gactttcatg ccatcagggg aaagacagac cgaaaatccc
     2701 cctcgaaaga cccgagaaaa aaggggggaa gtagtaactc tcctgtggtt tcgtcgccga
     2761 ggattgtatc ctcggcgcaa gtcgattgtt tctcgggtac ccgaacaaag gaccacggcg
     2821 ggactgttga ggttggtcgg tgcgactcta tcgagaaacc tcggttacgg gataagaaac
     2881 aggggcggct gaagccaccc ctcccggaga gcaaattgat aggcatcagg ttacgcaaag
     2941 tgccttgaga ggggcattaa aggaagggct gcagacagcc gaaaacagga caaaggagtt
     3001 tcgttaccat cctgggtacc tccgaccggg cgttgaccgg ggaaggtgtt tgtgctatgc
     3061 ctgtcgccac tgaaaagaaa gtcaaaacag ctaaaattct gatcatcgac gatgagccgg
     3121 agatcaccga gatcgtccag acctttctgg agaatgccgg ctaccaggtg atggtcgaaa
     3181 actcctcggt gatggggatt gagcgggcca agaagggatt gcccgattta atcctcctgg
     3241 acatcatgat gccgttcatg gacggctacg gagtctgcaa cgaactgaag aaaaacccgg
     3301 ccaccggcaa tatccccgta gtgttcctga ccggcaagga tacccgggat gacagcggcc
     3361 gctccttcaa agtgggcggc gacatgttca tcaagaaacc gttctcctgc gaacggctct
     3421 tggaaatcgt caaaatcgtc ctgcaaagcc tgaacaagta gttccacttt cccacacagg
     3481 ccgtttccga ccggcgtctc atccgcctat attgggggca tggtccggcc cgggctgctc
     3541 gcgtttcttt ttttctgcct tctttcctct ttcttttctg cggttagctg ggccgaggaa
     3601 tatcccgggc cagtcggcta tgtgaacgat ttcgccgggc ttttggccga agctcctgct
     3661 ggggctctgg aacagcaact ccgccagttc gaagaaagta ccggcgtgga ggtcgccgta
     3721 gccaccgtgg cgaccaccgg cgggctggcc gtgcaagact acgccaacga actgttcaaa
     3781 agatgggggg tcggaaaaaa ggggaaagat aacggtgtcc tcctcctggt ggcgagagac
     3841 gaccggaatg tctggataga agtgggttac ggcctggagc cggtgctgcc ggatgcctta
     3901 gtcggtcaaa tttttctcaa cgaaatccga ccgcgcttcc gggcggggga tttttcggga
     3961 ggcatcgtcg cgggagtcaa cgccctccag gcggcgattg aggggaagta tacagggcag
     4021 ccgtcaaaga aacgcccacg gtcggttgtt gcatatccaa ttcttctcgt catgctcttc
     4081 attggagttc tggtgttgtc gtcttttgta tcgggaggac gcagaggtgg gaaacgttct
     4141 ggctggattt ttct
//
LOCUS       DBNGZD010000126         2649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_508243, whole genome shotgun sequence.
ACCESSION   DBNGZD010000126 DBNGZD010000000
VERSION     DBNGZD010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_508243"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..303)
                     /gene="rpmA"
                     /locus_tag="AC16GM_03475"
     CDS             complement(37..303)
                     /gene="rpmA"
                     /locus_tag="AC16GM_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009870504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR2922768.1"
                     /translation="MAHKKGVGASRNGRDSHGQRLGVKAYGGEFVPAGSVIVRQRGTR
                     IYPGLNVGMGKDNTLFARITGTVHFETKGGDKKMVRVEPLPQPA"
     gene            762..2426
                     /locus_tag="AC16GM_03480"
     CDS             762..2426
                     /locus_tag="AC16GM_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922769.1"
                     /translation="MSRRVESSTSLRIVFLSALVCLLMASTRTAALPQLINFQGKLTD
                     AVGNPVADGSHTVNFSIFNVPSGGAFLWSEATSQTTAGGLFTHLLGSVTPLPLSLFVN
                     NDSLFLEIVADGQTISPRTRLASVAYALRVGTVDGASGGNLSGDLTVSGNVGIGAAPG
                     FQTKLDVQTSGNNSFIPIKGIQGVAENTSSGPAEGGYFSVGPSGTGAHYGVYGQALGS
                     ASSSYGVYGEGPTGVYGTGGTYGVYARSTGTAAVFGDIGGNFGTYGGYFQGDVKVERN
                     LVVNGTTSSSAGVYASSSSGAGVVASGSTNGVEATGGTRGVYAGGGTYGVVGFATSNV
                     FPSYGVYGSDNGTPVTTYAGFFSGQVKITSLLEVVGFIFKDGGGFKIDHPTDPANKYL
                     YHSFVESPDMTTLYNGNITLDAKGEATVQLPDWFEALNGDFRYQLTAIGAPGPNLYIA
                     REVSGNQFKIAGGTPGMKVSWQVTGIRQDAYAKAHRIPVEEEKPAAERGKYRHPKEQG
                     MPETLGMNYEETQKMEVERKQQEEQRAKMEQERLLMEQKTKANGNK"
     gene            2445..>2649
                     /locus_tag="AC16GM_03485"
     CDS             2445..>2649
                     /locus_tag="AC16GM_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922770.1"
                     /translation="MKRTLLFVVGLAAFLSQIAMTAVPNLINYQGKLTDGSGNPITNP
                     TQAVIISIYNVASGGSPLWAETLS"
BASE COUNT          595 a          786 c          697 g          571 t
ORIGIN      
        1 cacctgtagg ggtttgatta atcaaacccc tacgccctac gctggttggg gcagcggctc
       61 cacgcggacc attttcttat ccccgccttt ggtctcgaaa tgaacggtgc cggttatccg
      121 agcaaaaagg gtgttgtcct tgcccatgcc gacgttcagt ccggggtaaa tccgcgtccc
      181 gcgctggcgg acaataaccg accccgccgg gacaaactcc ccgccgtagg ccttgacgcc
      241 gagcctttgc ccgtgcgaat ctctgccgtt ccgcgaagca ccaacacctt tcttatgcgc
      301 catcgaattg cctccttaaa tctggagtag tatacgaaac tctcctcgcg aacacaagcc
      361 gccgctggcc accgcaggat tgaccgccaa gtcgtactta tcactccctt ctgtccccct
      421 tgataagggg gacagccctg agcagagtcg aagggtcagg gggtcaacaa aaaatcgccg
      481 gggtaaccca ctgggtcgcc ccacctttca attcaagacc ttctcgctct tgacaagaat
      541 ccccagcggg ttaaatttaa cttggtacac ggacatggcc cgtacgggtc gagggagggg
      601 tgcccaaaga gtagttcaac catttcctgt tcgagtcggt acaactcgga tgagaaggtc
      661 gtatgcttaa cgaattcggt tctccgcgca ggtccgcttt aagtggagcg attacccgtc
      721 aactgtttgt tcaaaacact tctcacaaga aaggagatac catgtctcgc agagtcgagt
      781 cttcgaccag tctccggatc gtattcttat ccgcattagt ttgtctgttg atggcatcaa
      841 cccgcaccgc cgccctgcca caactgatca acttccaggg gaaactgacc gacgccgtcg
      901 gcaacccggt cgccgacggc tcccacaccg tcaacttctc catcttcaac gttccctccg
      961 gcggcgcttt cctgtggagc gaagccacct cccagaccac tgccgggggg ttgttcaccc
     1021 atcttttggg ttcggtcact cctctgcccc tttccctttt cgtgaacaac gattcgctct
     1081 ttctggaaat cgtggccgat ggccaaacga tttctccccg gacccggctg gcctcggtgg
     1141 cctatgctct ccgggttggc acggtcgatg gcgcctccgg cggcaacctc agtggtgatc
     1201 tgaccgtctc cggcaatgtc ggtatcggcg cagcccccgg attccaaacg aaactggatg
     1261 tccagacttc aggcaacaac tcctttatcc ctataaaagg gatacaaggt gtagcggaga
     1321 acacctcctc gggtccggcc gagggcgggt atttctctgt cggtccaagt ggtaccggcg
     1381 cccattatgg agtttatgga caggctcttg gttcggccag ctccagttat ggcgtttatg
     1441 gcgaaggccc cactggggtt tacggcaccg gcggcacata cggcgtttac gctcgttcta
     1501 ccggaaccgc cgccgtcttt ggcgacatcg gcgggaactt cgggacgtat gggggctact
     1561 tccagggaga cgttaaagta gaacgaaatc ttgtcgtgaa cggcaccact tcatcaagcg
     1621 ctggcgttta cgcctcatca tcgagcggcg ccggcgttgt cgcctcgggc agcacgaacg
     1681 gcgttgaagc cacaggcggc acgcgcggcg tttatgccgg tggaggaacc tatggtgttg
     1741 tcggttttgc gaccagtaat gtctttccct cctacggtgt ctatggctcc gacaatggga
     1801 cccccgtaac tacgtatgcc ggcttcttct ctggacaagt taaaataacc agtctcctcg
     1861 aggtagtcgg tttcattttc aaagacggcg gcgggttcaa aatcgaccat ccgaccgacc
     1921 cggccaacaa gtatttgtat cactcctttg tcgaatcacc cgatatgacc accctttaca
     1981 acggcaacat aacgcttgat gccaaaggtg aagcgacggt gcaactgccg gattggtttg
     2041 aggcattgaa cggcgatttc cgctaccagc ttaccgccat cggagctccg ggacccaacc
     2101 tatacattgc ccgggaagta tccggcaatc agttcaaaat tgctggcgga actcccggta
     2161 tgaaagtttc ctggcaggtt accggcatcc ggcaggatgc ctatgccaaa gcccaccgga
     2221 ttccggtaga agaggaaaaa ccggccgccg agcggggcaa ataccgacac cccaaggaac
     2281 agggtatgcc ggaaacctta gggatgaact atgaagagac ccaaaaaatg gaagtagaac
     2341 gcaagcaaca agaagaacaa cgagccaaaa tggagcaaga gcgcttgctt atggaacaga
     2401 aaaccaaggc gaacgggaat aagtaaaccg aaagtgagga gcgtatgaaa cgcaccttgt
     2461 tgttcgtagt agggctggcg gccttcttgt ctcaaattgc aatgacagcc gtaccgaacc
     2521 tgatcaacta ccagggcaaa ctgaccgacg gttcgggtaa ccccatcact aaccccaccc
     2581 aggcggtcat tatttccatc tacaacgttg cttccggggg ctcacccctc tgggcggaaa
     2641 ccctttccg
//
LOCUS       DBNGZD010000127         7284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_855158, whole genome shotgun sequence.
ACCESSION   DBNGZD010000127 DBNGZD010000000
VERSION     DBNGZD010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_855158"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1654)
                     /gene="groL"
                     /locus_tag="AC16GM_03490"
     CDS             complement(26..1654)
                     /gene="groL"
                     /locus_tag="AC16GM_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR2922771.1"
                     /translation="MPKLIEYNTTAREKLKRGVDKLADAVKVTLGPRGRNVVIDKKFG
                     SPTVTKDGVTVAKEIELEDHFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIFREG
                     LRNVTAGANPMDLKRGIEKAVGVVVGELKKISKPVPGKTEISQVGAISANNDKVIGDL
                     IADAMEKVGKDGVITVEEAKTTETKLEVVEGMQFDRGSLSPYFITNPDTMEAVLEEAL
                     ILIHDKKISTMKDLLPILEKVAQVGKPLLIVSEDVEGEALATLVVNKLRGTLRVCGVK
                     APGFGDRRKEMLQDIAVLTGGKVISEELGFKLENTVMSDLGTAKRITIDKDNTTIVEG
                     GGKSSEIKGRVNSIKKQIEETTSDYDKEKLQERLAKLAGGVAVINVGAATETEMKEKK
                     ARVEDALHATRAAVEEGIIPGGGVAYLRCLAALDKIKENGDVAIGVNIVRKALEEPIR
                     MIAGNAGVEGSLVVNRVKEEKGAFGFNAENEKYEDLYTAGVVDPTKVARIALENAASI
                     AGLLLTTEAIIAEKPEEKKAPAMPPGGGYGGDMY"
     gene            complement(1784..2083)
                     /gene="groES"
                     /locus_tag="AC16GM_03495"
     CDS             complement(1784..2083)
                     /gene="groES"
                     /locus_tag="AC16GM_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR2922772.1"
                     /translation="MQVKPLADRVLIKPLETQETKKGGIIIPDTAKEKPQQGEITAVG
                     PGRITEDGKKIAMEVRKGDRVLYGKYSGTEVTIDDLECLIMRESDILAVIENGKK"
     gene            2485..3195
                     /locus_tag="AC16GM_03500"
     CDS             2485..3195
                     /locus_tag="AC16GM_03500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2922773.1"
                     /translation="MAKESIKDRAYYDQAGPYFERLKREYLQDTRWKRNRVRNVLNLA
                     RPQPGDVVLDLGCGIGTFVFESARRNARAYGIDFSITALSTAREIAAGQGLRVGGFVA
                     GDAGALPFQSESFTLIICADLVEHLYENTYRSMLAECHRTLTRGGRLAIYTPSPTHLF
                     ERLQHHEILLQKDESHIGLKTMPYLLETLRQAGFVIPHYYFRPTHLPVYRWVESCFIP
                     IPGLGRPFRRRSCILAMK"
     gene            3286..5751
                     /locus_tag="AC16GM_03505"
     CDS             3286..5751
                     /locus_tag="AC16GM_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922774.1"
                     /translation="MAKKKVHAKGCGRLQPAAPGGIVLAPPLQRLLPVVAYAIFTLLL
                     FREFIFSKGMLYGADTFQAGVFFRNFFVQFVKTFHELPQWDPYLFCGMPYLDAFHGDI
                     LYPLSLPFKFLLPLSRALGWILVFHIFLAGCFMYLCARGFGLGKFASFFAGTVFMLSS
                     YLVSLVHPGHDGKIFITALFLLALFFLHRGLEKKRAAFFVYFGVVVGVIIITPHPQMA
                     YFSLWGLGAYTVFRLVIHLRTEKKILETARLGVFTALAVLLALAISAIQFWPGYVYVK
                     QYSPRAEGAKDFAWATSWSLHPEELVSQVVPEFSGQHLGTGKNDYWGRNPFKDNSEYA
                     GLIPLLLAGIALVGARRKEAWFFCGLGLFVLIYALGATTPFFHFFYAVIPNIRNMRAP
                     SMIMFLFAFCSSLLGAMGLEEVFRRIKQGKLGESQKVVHYLWGATALLGLGAIIVTFS
                     GQGFAGLWKSIFWTTMPPERLVQLRLNLPRITLGFWLATFFVGICAFAIQRGLKGVWK
                     PTLVGAIIWTVAFFDLWRMDGRFIYVVDPQRVFGLHPSVEFLKQDPEPFRCFMQNDNF
                     SRDYFPYYGIELVSGYHGNQLRWYQEFLEAAQSETLGGFRFWNLINTKYFILPHGVDI
                     PRDPALGLDKVYDEQNVTIYRNLNAQPRAFVVHQIEVIPDREKILPRLADPTFDYRRK
                     IILEEDSKVALPPADTLATAEPVRISDNRINSFTVTATLDRPGFLFLSDTYYPAWKVL
                     EGNRERTIYRADYAFRAVYLEAGTHVLRFVYDSALYRIAKQVTIFSTLFAFAFLGGDL
                     AWRRWKKKRGKIAIPPVSSDTGG"
     gene            5854..6204
                     /gene="hpf"
                     /locus_tag="AC16GM_03510"
     CDS             5854..6204
                     /gene="hpf"
                     /locus_tag="AC16GM_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR2922775.1"
                     /translation="METRVTARHFELSDPLRRLAEEHSARLRRFFDRIIENHWTLTRE
                     KSRQTAELTVKVYGTVLTSKVVSHDIVLAVEGAADKMERQLKKYKERLKEKDVKRIST
                     LKTGGEIRPGAAEY"
     gene            6215..7183
                     /gene="hprK"
                     /locus_tag="AC16GM_03515"
     CDS             6215..7183
                     /gene="hprK"
                     /locus_tag="AC16GM_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR2922776.1"
                     /translation="MELISVEQLYRDKKEYLELSVLTGNSGLSKLVLNNEVHRPGLAM
                     AGFLDRFASQRTQVLGETELAYLVSFDEKQRAEIAERIFGQNLPVVFVTKGLAPPADF
                     LEVAVRHETAVLSSRLTTTEFVSRISTYLDHLFAPQTTIHGTLVDVYGVGLLYTGKSG
                     IGKSECALDLVERGHRLVADDMVRIIRKTPEVIIGTGSELLGHHMEVRGIGIIDIEKL
                     FGIRAIRMQKRIEVEVRLVAWDEEAEYERLGVEDKFTTILGVEIPVVTIPLSPGKNIT
                     VISEVIAMNHMLKIYGEHTAQEFSKRLSEEIHRRKMTTTYLESDYE"
BASE COUNT         1413 a         2024 c         2002 g         1845 t
ORIGIN      
        1 cggggctttt ggttctttct gtagattagt acatatctcc gccatagcca ccgcctggcg
       61 gcatggccgg agccttcttc tcctcgggtt tctcggctat gattgcctcg gtggtcaaga
      121 gcaacccggc gatcgaggcg gcgttctcca gcgcaatccg cgccaccttg gtcgggtcca
      181 cgacacccgc ggtgtagagg tcttcgtatt tctcgttttc ggcgttgaag ccgaaggcgc
      241 ctttctcctc cttcacccgg ttgaccacga gggagccctc caccccggcg ttcccggcga
      301 tcatccggat cggttcctcc aacgccttgc ggacgatgtt gaccccgata gcgacgtcgc
      361 cgttttcttt aattttatct aaggccgcca agcagcgcag gtaggccacc ccgccgccgg
      421 ggataatccc ttcctcgacc gccgcgcgcg tggcgtggag cgcgtcttcg acgcgggcct
      481 tcttctcttt catttctgtc tcggtggcag caccgacgtt gatcaccgcc actccgccgg
      541 ccaacttcgc caggcgctcc tgcaacttct ccttgtcata gtcggaggtg gtctcttcga
      601 tctgtttttt gatcgaattc acccggccct tgatttccga cgatttgccg ccgccctcga
      661 cgatggtggt gttgtccttg tcaatggtga tgcgcttggc ggtgcccagg tccgacatca
      721 ccgtgttctc cagtttgaaa cccagttcct cggagatcac cttgccgccg gtcagaaccg
      781 cgatgtcctg gagcatctcc ttgcggcggt cgccgaaacc gggagctttg actccgcaaa
      841 cccgcagggt gccgcgcaat ttgttgacga cgagagtcgc caatgcctcg ccctccacat
      901 cttccgagac gatcaagagc ggcttgccaa cctgcgccac tttttccagg atcggcagaa
      961 gatctttcat cgtggagatt ttcttgtcgt ggatgagaat gagcgcttcc tccagcaccg
     1021 cttccatcgt gtcgggattg gtgatgaagt agggcgagag agagccgcgg tcgaactgca
     1081 ttccctccac cacttcgagt ttggtctcgg tggttttggc ttcctcgact gtgatgacgc
     1141 cgtctttgcc caccttttcc atggcgtcgg caatcaggtc gccgatcacc ttgtcattat
     1201 tggcggaaat ggcgccgacc tgggagattt cggtcttccc cggcaccggc ttggagattt
     1261 ttttcaattc tccgaccacc acgccgaccg ctttttcaat cccgcgcttc aaatccatcg
     1321 ggttggcacc ggcggtgacg ttcctcaaac cttcccggaa aatcgcctga gccaggacgg
     1381 tagcggtggt agttccgtca ccagccacgt cggaggtctt ggaggcgacc tccttgacca
     1441 tctgggcgcc catgttctcg aagtggtctt cgagttcgat ttccttggcc acggtgacgc
     1501 cgtctttggt gacggtgggg gagccgaact tcttgtcaat tactacgttc cggccgcgcg
     1561 gacccaaagt cacttttacc gcgtcggcca gtttgtccac gccccttttt aatttctccc
     1621 gggcggtggt gttgtattca attaacttcg gcatacacta aacctccgtt ttgtttttgt
     1681 ttagggtgtt ttggagtgtc gaagtttcga agtgtcgagg ttgaaagact ttgtccttaa
     1741 ctctgacact ccgacactac gatacttcga cacgcagttt ccttcacttc ttcccgttct
     1801 cgatcacggc gaggatgtcg gattcgcgca tgatcaggca ttccaaatca tcgatggtca
     1861 cctcggtacc cgagtatttg ccgtagagca cacggtcgcc ttttctgact tccatcgcga
     1921 tcttcttgcc gtcctcggtg atccgccccg gcccaacagc ggtgatctcc ccctgctggg
     1981 gtttttcctt ggctgtgtcc gggatgatga tcccgccctt tttggtttcc tgcgtctcta
     2041 agggcttgat cagtacccgg tctgccagcg gttttacctg cattgctttt ccctcctttg
     2101 gggttttatt aaattttccc tgagtgtgag tgaaatcgat ttacacctcg cacttttagc
     2161 actcattcga agagagtgct aacaaaatta aaacctccaa tgaatattgt caagcgaaaa
     2221 aaatgtcgga agggcatttt ttatttaact cattgtctga cagattgtta tagtggaatt
     2281 atgtgggctt agtacttttt agagcaatct taaaggagtg aactcaagtc cgttctggtt
     2341 cttttttttt ggggtcggta ggagtaatag ccctgcgagg ccgcaggtct tcgccaacta
     2401 tcgtttacca tttgaattga ataagactcc ttgccaaata attgggttct gtgtgtattg
     2461 atcaatttgg agacgatagg gagaatggca aaggaatcga ttaaagatcg cgcttattac
     2521 gatcaagccg gtccgtattt cgagcgcctg aagcgggagt acctccaaga cacgcgctgg
     2581 aagcgcaacc gcgtccgtaa cgtcttaaac ttagcccgac cgcaaccggg cgacgtggtg
     2641 ttggatttgg gctgcggcat cgggactttc gtttttgaaa gcgcccggag gaatgcccgg
     2701 gcctatggta tcgatttttc gataaccgct ctttccacag cgcgcgaaat tgctgccggt
     2761 cagggcttgc gggtgggcgg ctttgtcgcc ggggacgccg gagcactccc gttccagagc
     2821 gaaagtttta cgctcatcat ctgtgccgat ctggtggaac acctctacga aaatacctat
     2881 cggagtatgt tggccgaatg ccaccgcacc ttgactcgcg gtggtcggct ggccatttac
     2941 accccgtcac cgacgcacct gttcgaacgg ctgcagcacc acgaaatctt gttgcaaaaa
     3001 gacgaatccc acatcggcct caaaacgatg ccgtatctcc tggaaactct ccggcaggcc
     3061 gggtttgtca tcccgcacta ctactttcgc cccacacatc tgccggttta ccgctgggtg
     3121 gagtcttgtt tcataccgat accgggtctg gggcggccct tccggaggcg gagttgcatt
     3181 ctggcgatga agtaggtcgt cccccccttc gtgtttttaa aaacgaaact ggccgtctgc
     3241 gagttttggc ttgacaggaa ggacttcttt ctgcctattt aacctgtggc gaaaaagaaa
     3301 gtccacgcga aaggctgtgg acgactgcaa cccgccgcac ccggcggtat cgttcttgct
     3361 cctcccctgc agcgtctgtt accggtggtt gcctacgcta ttttcacgct gcttttgttc
     3421 cgcgagttca tcttctccaa gggcatgctt tacggggccg acacgttcca ggccggggtc
     3481 ttcttccgta atttctttgt tcagttcgtc aaaactttcc acgaattgcc gcagtgggat
     3541 ccctatcttt tctgtggcat gccgtacttg gatgccttcc acggcgatat tctctacccg
     3601 ctctcactcc ccttcaaatt ccttttgccg ctctcccggg ctctgggctg gattctggtc
     3661 tttcacatct tcctggccgg ttgcttcatg tacctctgtg cgcgcggctt cggtctgggg
     3721 aagtttgcca gttttttcgc cggaacggtc ttcatgctct ccagttatct ggtttcgttg
     3781 gtccatcccg gacacgacgg gaagattttc atcaccgccc tgttcctttt ggcattgttt
     3841 ttcctccacc ggggattaga aaagaaacga gcggcattct tcgtttattt cggggttgtg
     3901 gtcggggtca tcatcatcac gccgcacccc cagatggcgt atttttccct ctgggggctg
     3961 ggcgcctaca cggtcttccg cctggtcatc cacctgcgca cggagaaaaa aattttggag
     4021 acggcccggt tgggggtgtt taccgcactg gccgttcttt tggcactggc gatttcggcg
     4081 atccagttct ggccggggta tgtctacgtg aaacagtact ccccgcgggc ggagggagcc
     4141 aaagacttcg cctgggctac ttcctggtcg ctccatcctg aggaattagt gagtcaggtc
     4201 gtgcccgaat tctcgggcca gcacctggga acggggaaaa acgactactg ggggcgcaat
     4261 ccgttcaagg ataactcgga gtacgccggc ttgatacctt tacttttggc cggcatcgct
     4321 ctggtcggcg cccggcgcaa agaagcctgg tttttctgcg ggttgggtct ttttgtcctg
     4381 atctacgctt tgggagccac gacccccttt ttccatttct tttacgccgt catccccaac
     4441 atccgcaaca tgcgggcgcc gtcgatgatt atgttcctgt ttgcgttctg ctcgtcgctt
     4501 ctgggagcga tgggattgga ggaggtgttt cggcggataa agcaagggaa gctgggcgaa
     4561 tcccaaaagg tagtccacta cctgtggggg gcgacagcgc ttctggggct gggtgcaatt
     4621 atcgtcacct tctccgggca gggattcgcg gggctgtgga agtcgatttt ctggacgacg
     4681 atgcccccgg agcgcctcgt gcagttgcgg ttgaatttac cccgcattac cctgggattc
     4741 tggctggcca ctttttttgt cggtatctgc gcttttgcca ttcagcgcgg attgaaaggg
     4801 gtctggaaac caaccttggt cggtgcgatc atctggaccg tggctttttt tgacctctgg
     4861 cggatggacg gccgcttcat ctacgtggtc gatccccagc gggttttcgg attgcacccg
     4921 tcggtggaat tcctcaaaca ggatccggaa ccctttcgct gctttatgca aaacgacaac
     4981 ttttcccgtg attatttccc ctattacggc atcgaattgg tttccggcta ccacggtaac
     5041 cagctccgct ggtatcaaga gttcctggaa gcggcgcagt cagagactct gggtggtttc
     5101 cggttctgga acctcatcaa caccaagtac tttattttac cgcacggggt tgatattccg
     5161 cgcgatccgg ctctggggtt ggacaaggtc tatgacgaac agaatgtgac catctaccgc
     5221 aacctgaacg ctcagccccg ggcttttgtg gtacaccaga tcgaggtcat tcccgaccgg
     5281 gagaaaattt tgccgcgctt ggccgatccg accttcgatt accgacgaaa aatcattctc
     5341 gaagaagact caaaggtcgc cctcccgccg gccgatactc tggcaacggc tgagccggta
     5401 cggattagcg ataaccgcat caattctttc acagtgacgg cgactctgga cagaccgggt
     5461 tttctctttc tgagcgacac ctactatccg gcctggaaag ttctggaagg gaaccgggag
     5521 cgcaccatct accgggcgga ttacgccttc cgcgcggtct atctcgaagc ggggacgcat
     5581 gtcctccgct ttgtgtacga ttccgccttg taccggatcg ccaagcaggt cacgatcttt
     5641 tccacgcttt ttgcttttgc ctttctgggc ggggatctgg cctggcggcg ctggaagaaa
     5701 aaaaggggaa aaatcgcaat acctcccgtc tcttctgaca cggggggata gaaaatgcgt
     5761 atattaattc agggaataag atgcgggtac gattcgcgag agatgtccac ctcttaaccc
     5821 atcacctttc aatgacttaa gaaaggagcg gttatggaaa cccgtgttac cgcgcgccat
     5881 tttgagctat ctgatccttt acggcgatta gccgaagagc attctgcccg cctgaggcgg
     5941 ttctttgacc ggatcataga aaaccactgg accttaaccc gggaaaagtc gcgccagacc
     6001 gcagagttaa ccgtgaaagt gtacggcacg gtgctgacga gcaaagtcgt ttcgcacgat
     6061 atagtgctgg cggtggaagg ggccgccgat aagatggaaa ggcagttgaa aaaatacaaa
     6121 gagcggttga aggaaaagga cgtgaagaga atatccaccc tgaaaacggg tggagagatt
     6181 cgtcctggag cagctgaata ttgatgggcg aagcatggaa ctgatttcag tcgaacagct
     6241 ctaccgcgat aaaaaagagt atctggaact ttccgttttg accggcaact cgggcctctc
     6301 caagctcgtt ttgaacaatg aggtgcaccg gccggggctg gcgatggccg ggtttctgga
     6361 ccgctttgcc agccagcgga cgcaggttct gggcgaaacc gaactggcct accttgtcag
     6421 tttcgatgaa aagcaacgcg cggagatcgc cgagcggatt ttcggacaaa atctgccggt
     6481 ggtcttcgtc accaaagggc tggcgccgcc cgccgatttc ctggaggtgg cggtgcgtca
     6541 cgaaaccgca gtattaagct cgcgtttgac gaccactgaa ttcgtttccc ggatttcgac
     6601 ctacctggat cacctctttg cgccgcagac caccatccac ggcaccttag ttgacgtcta
     6661 cggggtgggg ctgctctaca ccggcaaaag cggcatcggc aaaagcgagt gcgccctcga
     6721 tctggtggag cggggccatc ggctcgtcgc cgacgacatg gtgcggataa tccggaagac
     6781 gccggaagtg atcatcggca ccggctccga acttttgggc caccacatgg aggtgcgcgg
     6841 catcgggatc atcgacatcg agaaattatt cggcatccgc gccattcgga tgcagaagcg
     6901 aatcgaagtc gaagtgcgtt tagttgcctg ggacgaagaa gcggagtacg agcggctggg
     6961 ggtcgaagac aagtttacca cgattctggg agtggagatt ccggtggtga cgattccgct
     7021 ttcgccgggc aagaacatca cggtgatctc cgaggtaatc gccatgaacc acatgctcaa
     7081 aatctacggc gagcacacgg cccaggagtt ctccaaacgg ctttccgaag agatacaccg
     7141 gcgcaagatg accaccacct acttggaaag cgactacgag taagcaggta gcaggtggtg
     7201 ggtgagaggt ggtaggggga aaaagttcat ctctgggggc tgattgtatt cagcccctcc
     7261 attttgagca catcccttac ggtt
//
LOCUS       DBNGZD010000128         4079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_160620, whole genome shotgun sequence.
ACCESSION   DBNGZD010000128 DBNGZD010000000
VERSION     DBNGZD010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_160620"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1713
                     /locus_tag="AC16GM_03520"
     CDS             154..1713
                     /locus_tag="AC16GM_03520"
                     /inference="COORDINATES: protein motif:HMM:NF025699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR2922777.1"
                     /translation="MRKVLMNQTGAAMLVAMVVLVMLAFIGIAAVTTSLTDSDISHNY
                     QKKTEAFYTAEAGLEYGLKTMQNNVTILDSAQLMNMINASNTLGRGTFTVTMGNDLPV
                     RTLISSGRNTDATSAQAVDVQYKLNPLSVWNNVLFAGVGQSGKAINGNVGFHGSVHIQ
                     GNGDRFTDTNGNGIHDPLEPYTDDNGDGVYTAPLTSSDVALDIGGGAGVYNNYNGMPV
                     SLSSRVPALGQVPFGGENVSSLDAEFRVQHGLVTINSNASQIGEPDVSGGSPPVKETM
                     DGVYVNDGFGGSNPTNVYADNGTSEKYDLGGGQLSFPNLNDPYTDGGVTYANYTAYLL
                     AHALVIGSDLILEAGTAMAKITGPNGSSIELDDQGHLNISGIVYVTGNITIQQGKGGS
                     GNNPIIYDGRGTLAARNDISITNNLLSKGAFPTDDVIGLVAGHDMTIAGSGASHLELT
                     GAFFAQHQMNNSKQNELAGAMVSNYFNITNVPHFYQVPSLKDNLPPGMPGAGTVTTYT
                     WKKRFASWREL"
     gene            1732..2211
                     /locus_tag="AC16GM_03525"
     CDS             1732..2211
                     /locus_tag="AC16GM_03525"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR2922778.1"
                     /translation="MRKLRKNRGFTFVELIVAVAVVGVLAALAAPRFFRTMTQLKAKG
                     AARDAVSVLRLARSQAVSKKIRYGVLFELDNRRITYFRDDNSSGTYDTGDSLLTREVL
                     STDVTLSNSTLTNNTVVFTSTGQATGSGAVRVQTNDGTWRYAVDVLASTGRVRLTAL"
     gene            2497..3555
                     /gene="pilM"
                     /locus_tag="AC16GM_03530"
     CDS             2497..3555
                     /gene="pilM"
                     /locus_tag="AC16GM_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR2922779.1"
                     /translation="MITDLLRRSQNVAALDIGSGAIKLVQLEKKSGGYSLKSLGVREL
                     PLEAIVADEIKDRDTVIFNIQSLVDQCDPHTKEVVVSLAGHGVITDKISIDKKSGSEA
                     EQAILFEAEQRAPFDVEDVTLDYHVIAVNEETNKMDVLLVAARNEYLRSFLDVIADAG
                     LRAVVVDIDAFAILNAYEANYEIDPQKVTALVNIGFDATNITFLKDGQYHSTREVSAG
                     GRIIYDAIQKEFRLNHELTAKAIRGELEGTVDADMLRVTVISAAEELLSGLEVAFSYF
                     KSSAKVPAVEWIMLSGGGALVPFLPEFMQSKFATPVEIANPLRNIDYDPDQFRHLDLE
                     RIAPLLMVPIGLAARRVK"
     gene            3558..>4079
                     /locus_tag="AC16GM_03535"
     CDS             3558..>4079
                     /locus_tag="AC16GM_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR2922780.1"
                     /translation="MIEINLLPKDLRKRSHVIRLPRTAWYVSVGVGALLVAVVGATVM
                     QRYQMKKWQTRIQKTRTQVEQMRRDIQVVDALEEVKAKILDRLSAIEQLDRNRAVYVS
                     MFEDLARRVPEYLWLTTFKEGLAQPAAAAPGGNAPPTAPSPGASAGSASPQRTKMEGF
                     AYSLNSIATFLIQM"
BASE COUNT          888 a         1230 c         1232 g          729 t
ORIGIN      
        1 cagatgctca agggcaacca gtacgtgaat tacacccgcc ggctggtctc cagcgtcaag
       61 ctcaagaacc ggtagcggag caggcacttt gaaaaacgac agcaatggcc cagagaatcg
      121 atgacgaaaa gagagaacaa aggagcgaga cgtatgcgta aagtcctgat gaaccaaacc
      181 ggagcggcga tgctcgtcgc catggtggtg ctggtgatgc tggccttcat cggcattgcc
      241 gccgtcacca cctcgctgac cgatagcgac atttcccaca actaccagaa gaaaaccgag
      301 gccttctata cggctgaggc gggactggag tacgggctga agacgatgca gaataacgtc
      361 accattctgg acagcgccca gctgatgaac atgattaacg ccagcaatac ccttggtcgc
      421 gggaccttca cggtgacgat ggggaacgac ctgccggtcc ggaccttgat ttcctccggc
      481 cggaacacgg acgccaccag cgcccaggcc gtcgatgtgc agtacaaact caacccttta
      541 agtgtctgga acaacgtgct ctttgccggc gtggggcaga gcggcaaggc gatcaacggc
      601 aacgtggggt tccacggctc agttcacatt caggggaacg gcgaccgctt caccgacacc
      661 aacgggaacg gtatccatga tccgctcgaa ccgtacaccg atgacaatgg cgacggcgtc
      721 tacaccgccc ctttaacttc ttccgacgtg gcattagata tcggtggtgg agccggggtg
      781 tacaataact acaatggaat gccggtctcg ctctcctcgc gcgtaccggc tctggggcag
      841 gttcccttcg gcggcgaaaa cgtttcctcc ctggacgccg aattccgggt ccagcacggc
      901 ctcgtgacca tcaactccaa cgcgtcgcaa atcggagagc cggacgtgag cggcggctca
      961 cccccggtga aagaaacgat ggacggggtc tacgtcaatg acggctttgg cggttcgaat
     1021 cccaccaacg tttacgcgga caacggcacc agcgaaaagt acgacttagg cggcggtcag
     1081 ttaagctttc ccaatctgaa cgacccgtat accgacggcg gagttaccta cgccaactac
     1141 acggcttacc tcctggccca tgcgctggtg attgggagtg acctcatcct ggaggcggga
     1201 acggcgatgg cgaagataac cggccccaac ggaagcagca tcgagctgga tgaccagggt
     1261 cacctgaata tttccggcat cgtttacgtg accggcaaca taacgatcca gcagggtaag
     1321 gggggcagcg ggaataatcc gatcatctac gacggccgcg gcacgctggc ggccaggaac
     1381 gatatttcga tcacgaataa cctgctctcg aagggcgctt tcccgaccga cgacgtcatc
     1441 gggttagtcg ccgggcatga tatgaccatc gccgggtcgg gcgcttcgca cttagagctg
     1501 accggggcct tcttcgccca gcatcagatg aacaattcga agcagaacga actggctggg
     1561 gccatggttt ccaactactt caacatcacc aacgtgccgc acttctacca ggtaccgtcg
     1621 ctcaaggaca acctcccgcc cggcatgccc ggtgcgggta cggtaacgac ctacacctgg
     1681 aagaagcggt tcgcctcctg gcgggaactg taaaggaaac gagagggtgc tatgcgaaaa
     1741 ctgaggaaaa atcgcgggtt cacgttcgtc gaattgatcg tggcggtggc cgtggtcggg
     1801 gtgttggccg ccctggccgc gccgcggttc tttcggacca tgacccagct caaggccaag
     1861 ggggccgccc gggatgcggt ctcggttctg cgcttggccc gctcccaggc ggtgagtaag
     1921 aaaatccggt acggcgtcct cttcgagctt gataaccggc ggatcaccta cttccgggac
     1981 gacaacagtt cgggaaccta cgacacgggc gattccttac tcacgcgaga ggttctgtcg
     2041 accgacgtga cgctctccaa cagcaccttg accaacaaca ccgtcgtttt tacttccacc
     2101 ggccaggcca cgggctccgg cgccgtccgc gtccagacca atgacggcac ctggcgctat
     2161 gctgttgacg ttttggcctc caccggccgg gtacgcttga ccgcccttta acgcagttcg
     2221 tccgccggac cacccaaaaa cgtccccgat ttccgggacg tttttgggtt tcgtccatgg
     2281 gtgccctggg taccgtctcc cgccgccgcc cctggcagac ggggaggggt ccccattggg
     2341 tggggagcaa aaaaagtttt tgggggtgct caatattttg gcaggggggt cgataatctg
     2401 aaagtggaaa gcaggttaac cgccagcggg tggggcagca ccggagaggg aaaagcggtg
     2461 gccccggagc gccggcccaa aacggactga agacacgtga tcaccgatct cttacgcaga
     2521 agccagaacg tcgccgcgct ggacatcggc tccggcgcca taaagctggt gcagctggag
     2581 aagaagtccg gggggtacag cctgaagtcc ctgggcgtgc gcgaactgcc actggaggcc
     2641 atcgtcgcgg acgagattaa agaccgcgac acggtgattt tcaacatcca gagtctggtc
     2701 gatcagtgcg acccgcacac caaagaggtg gtggtgtcgc tcgccgggca cggcgtcatc
     2761 accgataaga tctccatcga caaaaaatcg ggtagcgaag ccgaacaagc gattctcttc
     2821 gaagccgaac agcgggcgcc cttcgacgtg gaggacgtga ccctggacta ccacgtcatt
     2881 gcggtcaatg aagagaccaa taagatggat gtcctcctag tggccgcccg caacgagtac
     2941 ctccggtcgt ttttggacgt catcgccgac gccgggctcc gggcggtggt ggtcgatatc
     3001 gacgccttcg ccattctcaa cgcctacgag gcgaactatg aaatcgaccc acaaaaggtg
     3061 accgcgctgg tgaacatcgg cttcgacgcc accaacatca ccttcctcaa agacggccag
     3121 taccactcca cccgcgaagt gtcggccggg ggacggatca tttacgatgc gattcagaaa
     3181 gaattccgct tgaaccacga actcaccgcc aaggcgatcc gcggcgaact ggaaggcacc
     3241 gtggatgccg acatgctgcg ggtgacggtc atttccgcgg ccgaagagct cctctccggc
     3301 ctggaagtgg ccttctccta cttcaaatcc tcggccaaag tgccggcggt ggagtggatc
     3361 atgctgtccg gcggcggcgc cctggtgccg tttctgccgg agttcatgca gtcgaaattc
     3421 gccacccccg tggaaattgc caatccgctc cgcaacatcg actacgatcc ggaccagttc
     3481 cggcatctgg acttggaacg catcgcgccg cttttgatgg tgcccattgg cctcgcggcc
     3541 cgaagggtga agtagccatg atcgagataa acctgctccc caaagatctc cggaagcggt
     3601 cccacgtcat ccgcctgccc cgcaccgcct ggtacgtctc ggtgggggtc ggggcgctcc
     3661 tggtagctgt ggtcggggcc acggtgatgc aacggtacca gatgaagaag tggcagacgc
     3721 ggattcagaa aacccgcacg caggtggaac agatgcgccg cgacattcag gtggtggatg
     3781 ccttggagga agtgaaagcc aaaattttgg accggctgtc ggccatcgaa caactggacc
     3841 gcaaccgggc cgtgtacgtg agcatgttcg aagacttggc gcggcgggtg ccggagtacc
     3901 tgtggctcac cacgttcaaa gagggtctgg cccagccggc ggcggccgct ccgggcggga
     3961 atgctccgcc cacagcgccg tccccgggtg cgtcggcggg ttcggccagt ccgcagcgga
     4021 ccaaaatgga agggttcgcc tactcgctca acagcatcgc cacattcctg atccagatg
//
LOCUS       DBNGZD010000129         4852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1202721, whole genome shotgun sequence.
ACCESSION   DBNGZD010000129 DBNGZD010000000
VERSION     DBNGZD010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1202721"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="AC16GM_03540"
     CDS             <1..211
                     /locus_tag="AC16GM_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922781.1"
                     /translation="LARKFLEIEGLLFRFEESRLELPESLFFQKFFFAVEQKEAAQFA
                     GSYLPHQIGVRFDLSLSRWHRLPGC"
     gene            complement(443..826)
                     /locus_tag="AC16GM_03545"
     CDS             complement(443..826)
                     /locus_tag="AC16GM_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HIR2922782.1"
                     /translation="MQCYFYGVTRREKKSAQPGGVASFSIPDLGLSFSAQFDGNREEC
                     QYAGLLALLEFVELNSNLFRKKTLEIYGDSFIVVHQVNLKMVCTRTLEPYRNLALVYK
                     KKISYTLNWIPPSQNRAQPGLALQA"
     gene            complement(1099..2262)
                     /locus_tag="AC16GM_03550"
     CDS             complement(1099..2262)
                     /locus_tag="AC16GM_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR2922783.1"
                     /translation="MIDVQTTRLRLEETEAHLLAPYATRNRDSRGRVYPEEEHPYRTR
                     FQRDRDRIIHSTAFRRLEYKTQVFVNHEGDHYRTRLTHTIEVSQIARSLARALRLNED
                     LAEAIALVHDVGHTPFGHAGEEVLNELLKDSGGFSHNRQTLRVVDLLEERYPEFPGLN
                     LTYEVREAIVKHGTSYGSPYPQEFSAKEQATLESQLVNIADEIAYNCHDVDDGLYSGI
                     LSVEELRQVELFRRLEEQARADFPQATPGRLQNVIVRRLINYEVSDVVTATLENLKAK
                     GVDSLEKVRDAGETLVGFSDSLAALNRELKEFLFRRMYRHFRLVRMADKARRIMTALF
                     AVYRDDPDQLPPTTRARLSREPVHQVIADYIAGMTDRFALLEYKKLFDPLERV"
     gene            complement(2303..2698)
                     /locus_tag="AC16GM_03555"
     CDS             complement(2303..2698)
                     /locus_tag="AC16GM_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR2922784.1"
                     /translation="MRKKNFLTYPKSAVTKARKLRRGMTDAEKKLWTFLRRNYLGTHF
                     RRQVPFGPYILDFLAINARLAVELDGSQHYEPKGKTRDTKRDVFLKEQGLAVLRFTDR
                     EVLGNINGVMQRIIEHIQQDRFSLPLRGG"
     gene            complement(2849..3658)
                     /locus_tag="AC16GM_03560"
     CDS             complement(2849..3658)
                     /locus_tag="AC16GM_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922785.1"
                     /translation="MRRFFFLFSLCGILFSSEIARSAFELQPLSTRALALGLSRFAPT
                     PTCLFSQPALLTKTPRSFQADYFRLYDLAELSQGVVAANLPRGDFAFGLGVSSFGRSD
                     YYRENQVAFGVARRVNNFSLGAGAKFWQVALPAPYSSPTGIAADLGLTYDLSTLGRFG
                     SSLGNLSLVASDDHLYPPVEIALGFSSTIANQFFVTLSGQWDDETKTSLRFGQEYHVN
                     SNLTLRFGFLTDPARFTLGLGVGKGKLHIDYGFLSHPVFSGSHSVGVGVEW"
     gene            complement(3655..3843)
                     /locus_tag="AC16GM_03565"
     CDS             complement(3655..3843)
                     /locus_tag="AC16GM_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922786.1"
                     /translation="MRGRQIRTLVEQTPQASGRLTWDGKGEDGKRVRAGIYIVFAESV
                     GSQHFTHKTTVVVAKSRE"
     gene            complement(3816..4568)
                     /locus_tag="AC16GM_03570"
     CDS             complement(3816..4568)
                     /locus_tag="AC16GM_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR2922787.1"
                     /translation="MSQTFSDTLSLSTGPHRLWCVLDPDDRPENNRASMDYVVGDFLP
                     GVVLNEFLPDPTSAVGTEWIELVNRSAGPVNLKDWKIRDAAGDTLAFAESLFVSHSEF
                     LIAAKDKSLFAAAYPQVTSRVYQPATWLTLNNSGDRIMLIDARGFAVDSVSYANGWGG
                     NVSWERVDVNRPASASDNWWRSVAPSGSTPGQVNSLASGFAQGLTLDINPNPFSPDGD
                     GFDDETEISFSVPFASSLTLKILTCAGARSER"
BASE COUNT         1093 a         1277 c         1443 g         1039 t
ORIGIN      
        1 gctggcccga aagtttctgg agattgaggg ccttctcttc cggtttgaag agagccgcct
       61 cgaattaccg gagagcctct tttttcagaa attcttcttc gccgtagaac agaaagaagc
      121 ggcccaattt gcgggcagct atctcccgca ccagatcggc gtacgctttg acctttctct
      181 ctctcggtgg catcgactcc ctggctgttg aaatatataa cctgccggtg gacgcgttca
      241 aagcaaaaga gggctcaagg ggagtagcgc cggtcacctg accggcgtct ttgcctgaat
      301 gacctctcgg agcgagtctt cgcgaagagt cgcttcgaca ccctaccctc tcctccagga
      361 gaggaattat gaaataatga aaactccctc cccttgaggg gagggatcaa gggaggggtt
      421 gcttagaagt tatgaatagg acttacgcct gaagcgccag ccccggctgg gcgcggttct
      481 gactgggggg tatccagttc agagtgtagc tgatcttctt cttgtagacc aaggcgaggt
      541 tgcggtaggg ctccagggtc cgggtgcaga ccattttgag gttcacctgg tgtaccacga
      601 tgaaactgtc gccgtagatt tccagcgtct ttttgcgaaa aagattcgag ttcagttcga
      661 cgaactccaa cagcgccaga agccccgcat actggcactc ctcccggttg ccgtcgaact
      721 gggcgctgaa ggagagcccc agatcgggga tggagaagga ggcgacgccg cccggttgcg
      781 cgctcttttt ctctctccgc gtgaccccgt agaagtaaca ctgcatcggc aacttgactc
      841 tccaaaacgt caaaagtgca aaccctgtgc cgatggaaga ttagcgcaag cccgctctgg
      901 atgcggattt ccggagagaa ggggaaagcc gccggagcgt aagtgtatgt agaactgtaa
      961 gataagtgaa atggcctttt gtagaactcc tttgtgtggg ttggctcgat tacgagccca
     1021 aaacaccgct gcggaacacc gcaaaatcgc tatgcatagt cctacaggag aggtgcagag
     1081 agggttatac gcttgaggtc agactcgctc cagcggatca aacagtttct tgtattccaa
     1141 gagggcgaag cggtcggtca taccggcgat gtagtcggcg attacctggt gcaccggctc
     1201 ccgggagagc cgcgcccggg tggtcggggg taattggtcg ggatcgtccc ggtagacggc
     1261 aaagagggcg gtcattatgc gccgcgcctt gtcggccatc cgtaccaggc ggaagtggcg
     1321 gtacatccgc cggaaaagga attccttcag ttcccggttt aacgccgcca gtgaatcgga
     1381 gaaccccacc agtgtctccc cagcgtcccg cactttttcc agcgaatcga cgcccttcgc
     1441 cttgagattt tccagtgtgg cggtgaccac gtccgacacc tcatagttga tcagccgacg
     1501 gacgatgaca ttctgcaggc gcccgggagt ggcctgcgga aagtccgccc gcgcctgttc
     1561 ttcgagacgg cggaagagtt ccacctggcg caattcttct accgagagga tgccggagta
     1621 gagcccgtcg tcaacgtcat ggcagttgta ggcgatttcg tcggcgatgt tgaccaactg
     1681 gctttccagc gtggcctgct ctttggcgga aaattcctgc gggtacggcg agccgtaact
     1741 ggtgccgtgt ttgacgattg cctcgcgcac ttcgtaggtg aggttcaggc cggggaactc
     1801 ggggtagcgt tcttccaaga gatcgacgac gcgcaacgtc tgccggttgt gggaaaagcc
     1861 gccggagtct ttgagcagtt cgttcagcac ctcttccccg gcgtggccga agggggtgtg
     1921 gcctacgtcg tgcaccagcg cgatggcttc ggctaagtct tcatttaagc ggagggcgcg
     1981 ggcgagcgag cgggcgattt gcgagacttc aatcgtgtgc gtgaggcggg tgcggtagtg
     2041 gtcgccttcg tggttaacga agacctgggt tttgtattcc aagcggcgga aggcggtgga
     2101 gtggatgatg cggtcgcggt cgcgctggaa gcgggtgcgg taggggtgct cctcttccgg
     2161 gtagacgcgg cctcggctgt cgcggttgcg ggtggcgtag ggggccaaca gatgggcttc
     2221 ggtttcttcg agccgcaggc gggtggtttg gacatctatc atgttaacgt ctctttctcc
     2281 cgtttcaata ctgacccctc cctcaccctc ccctcaaggg gagggaaaac ctatcttgct
     2341 ggatatgctc aataatcctt tgcatcaccc cattaatgtt acccaggact tctctatctg
     2401 taaaccgcag gacagcaagt ccctgttctt tcagaaatac atcccgtttt gtatcacgag
     2461 tcttaccttt tggttcatag tgttggctac catctaattc tacagctagt cttgcattga
     2521 tggcaagaaa atcaaggatg taagggccga aaggaacctg ccgccgaaaa tgagtaccga
     2581 gataattacg tctcaaaaaa gtccagagct tcttttctgc atcggtcatc ccgcgccgga
     2641 gtttccgcgc tttagtaacc gctgactttg ggtatgtaag aaagttcttc ttcctcacct
     2701 ttaaacctca aaatcgtatc gcgcctaacc aacacttccg acctctcccc aaccctctcc
     2761 tcaaggagag ggagaatact ccacggctat caagaacgta tttctttccc tccccttgag
     2821 gggagggcag ggaggggtcg cttcatcttt accactccac tcccacccca acactatggc
     2881 ttccggaaaa gaccggatgg ctcaggaaac cgtagtcaat atggagtttt cctttgccca
     2941 cgccaagtcc taaggtaaag cgggcggggt cggtgagaaa gccgaagcgc agagtgagat
     3001 tcgagttcac atgatactcc tgcccaaagc ggagagaggt tttggtttcg tcatcccact
     3061 ggccggagag ggtgacgaag aattgattgg cgatcgtgct cgaaaagccg agcgcaattt
     3121 cgaccggcgg gtagaggtgg tcgtctgacg ccacaagcga gagattgccg agagaggagc
     3181 cgaagcggcc tagagtggaa agatcgtagg tcaggccgag atcggcggcg atacctgtcg
     3241 gcgacgaata aggagccggg agcgccacct gccagaattt ggctcccgca ccaagcgaga
     3301 aattgttcac ccggcgggcg acgccaaagg cgacctgatt ttcacggtag tagtcggagc
     3361 ggccgaaact cgacacccct agaccaaagg caaaatcccc tcttggaaga ttcgccgcca
     3421 cgacgccctg actcaattcg gcaaggtcat agagccggaa gtaatcggct tgaaacgagc
     3481 gtggagtttt tgttagtaat gcgggctgag agaagagaca ggtgggcgtc ggcgcaaagc
     3541 gggataaacc gagggcaaga gcgcgagtgc tcagcggttg gagttcaaag gcgcttctgg
     3601 ctatttccga agagaaaaga attccacaaa gactgaacag aaagaaaaag cgcctcattc
     3661 gcggcttttg gccaccacga cggtggtttt gtgggtgaaa tgctgagaac caacgctctc
     3721 ggcaaaaacg atgtaaattc ctgcccgcac gcgtttacca tcctctccct tgccgtccca
     3781 ggtgaggcgt cccgatgctt gaggcgtttg ctctactaac gttcggatct ggcgcccgcg
     3841 cacgtcaaga ttttgagcgt gagactggaa gcgaacggca cggaaaagga gatttccgtt
     3901 tcatcgtcga aaccgtcacc atccggcgaa aagggattgg ggttaatatc cagcgttagc
     3961 ccttgagcaa atccgctcgc cagagaattg acctgccccg gtgtggaacc ggacggcgcc
     4021 actgagcgcc accagttgtc ggacgcggag gcaggacggt tgacatcgac tcgttcccag
     4081 gaaacgttcc cgccccagcc gttcgcatag gacactgaat cgaccgcaaa gccgcgcgca
     4141 tcgatgagca tgatgcggtc gccgctgtta ttgagggtga gccaggtggc gggttggtag
     4201 actcgtgaag tgacctgcgg gtaagcggcg gcaaaaagcg atttgtcctt cgccgcaatc
     4261 agaaactcgc tatgggacac gaacaaactc tccgcaaatg cgagcgtgtc gcctgcggcg
     4321 tcacggattt tccaatcttt gagattgact ggccccgctg agcgattgac cagttcaatc
     4381 cattccgttc ccactgcgga agttgggtcg gggagaaatt cattcagcac cacacccggc
     4441 aagaaatcac cgacaacgta gtccatcgat gcccgattgt tctccgggcg gtcatcggga
     4501 tcaaggacgc accagaggcg gtgcgggccg gtggagagcg agagtgtgtc ggaaaaagtt
     4561 tgactcattc caatacctaa tggaggaacg gaaaaagtgc tcagtgattc cgtgaatact
     4621 agaatcgaat caaaatcgcg atcatcataa agaacaacgc ggttgggcag ggaagtggcc
     4681 gtgccgacgt ttttgacctc gcaagaaacg agaaaatgag aactgtcagg aaaactcgcg
     4741 agcagcgacg gcggaaaaag aagcggcgtc aaatcattgg gctgtggtga ctgagtattg
     4801 atccgtcctg gtgtgtttcc gggcggatta gtcgccggaa gccagagtgt at
//
LOCUS       DBNGZD010000130        11729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_369529, whole genome shotgun sequence.
ACCESSION   DBNGZD010000130 DBNGZD010000000
VERSION     DBNGZD010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_369529"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1247
                     /locus_tag="AC16GM_03575"
     CDS             156..1247
                     /locus_tag="AC16GM_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922788.1"
                     /translation="MFAWFGTSFPSRCGTTFFLAGFLGLTAFASPASRAADPPAELKL
                     PPHTAEILAGIDLSTREEYQKARAVFRSLVERDSTDPWGWFFLAAGLQDEMMDKENFI
                     WKEEFKQAVERTLLLAKARADRDKKDAWSLFILGNAYGYLTVFLARQGDWFSGMRAAV
                     KAKGYYEKALKINPQLYDAYVGLGNYHYWKSVRTKAFLWLPFFKDTRKQGIAELEKAA
                     RLGTFARTAACFSLMYIYIHEKRYDAALALADSLSREYPESRSFLWGQAAAYFENYHW
                     AEAARAYAELVHEIEEEPDQNYYNLIDCTYRIAQCRFNLGRFNDCITSCEKLLAYPAS
                     PEIVKRQEAKIRKTRALLEKAQKITGKKK"
     gene            complement(1251..2213)
                     /locus_tag="AC16GM_03580"
     CDS             complement(1251..2213)
                     /locus_tag="AC16GM_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922789.1"
                     /translation="MNTKPRFLFPGLGRALAAQRLLFFLWLANLVCAAALTYPLRAAF
                     ADAAGTSLAGRTLGEGFNFALMSDFIEHRGAALDAAFGLLGPALLFFLFLYTFFDGGI
                     IAQFNNDQNRPLLSSFLANCGKYWGRLLRLFLWFLPAVIVFYIITKIMSKIAGLVSAN
                     RGNEHLTIWAYGVAWVLSFGVWCFFSMIFDYAKIGLVVEDSRKTFAALRRSFRFVFRN
                     FGRTVRLYFSLVAIGLVFFLIYFLIEINAPTEKWGIFWLVLILQQLCLLSRIWLRLGF
                     YSCQTALYLRYADRPTSERLPAPAISREIAPPFPSAGERPELAA"
     gene            complement(2214..4292)
                     /locus_tag="AC16GM_03585"
     CDS             complement(2214..4292)
                     /locus_tag="AC16GM_03585"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR2922790.1"
                     /translation="MPIRKNTLRAFLFGLFLAGIFFPLPARAALSRQVVHYEITAKLD
                     TFPKAVIGQETVTWINDSPDHVSSLRFHLYLNAFKNEKSTFFKELGGRRRRGKLRGDK
                     WGYIDIQKMTLADGTDLLPTLRYEHPDDNNLDDQTVCSVKLPKPVPPGGQVTLRIEFF
                     SKLPEVFARTGYKRNFYMVGQWFPKLGVYEKAGMRGRKTGGWNCHQFHANSEFYADYG
                     SYRVTLTVPDGFKVGATGARTSESADPKAHTQTVTYLQEDVHDFAWTASPAFVVQPDT
                     HVFPSGHRVAITLLNQPDYAGQKQRLLEAAHQALKYFGRWYGEYPYATLTIVMPPTGA
                     FGAGGMEYPTLFTGITLVPADWPLLRNIHYLVEETIVHEFGHQYWYGLVGSNEFEESW
                     LDEGLNTYSTGKVLAAWFGDKASFGRCLGAAISELEFARWQVVQYPHYDPVNTFSWRF
                     FNGNSYGTNSYAKVQILLTTLENYLGPDRMENVMRTYFERFRFKHPATEDFVRTANEV
                     SGQNLDWFFNQFVYGTQKVDYAVAGIASQEIKPQRGFFDLDGRKVFVESGGDTTVLGD
                     TALTKGKTLAKAKDRNGKKKEVKVYESAVQIQRKGDGIFPVEIEIKFEKEKPFTYHWD
                     GEERWIKYRFRGPHELEYAAVDPQNKIVLDLNRLNNSRTIVKQSRAITRWSSRWLFVM
                     QNLLQTLAVF"
     gene            4391..4807
                     /locus_tag="AC16GM_03590"
     CDS             4391..4807
                     /locus_tag="AC16GM_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922791.1"
                     /translation="MTRNKRRTAASLLLLVLLLVLWVVFNPFGRFGWCSFGFTIYNAI
                     PRPVTDIQVRADGKVRRVAKTHDLRYEDIVWLLDPKPDLLIIATGWDGVTVPRPEIGN
                     LRDCEVRILKTEEARLLFNALKRSGKQVAIHFHSTC"
     gene            complement(4878..5693)
                     /locus_tag="AC16GM_03595"
     CDS             complement(4878..5693)
                     /locus_tag="AC16GM_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922792.1"
                     /translation="MRKTIVAVFILSLVVAATALAVDQSKLEQLPAKIEHGRTLTAFP
                     LSLGYACNLSFIYPVPDGVGDTYEGMRFDVPAGKTFTLGTVDVLLYNRPDSTNDTVNN
                     VVVYVWGDSPSCPPGVPDTTRLLACDTITPAEQLAPGPWPPNGVQSVASDFTSKNLHF
                     YSGQKFHITLMNLPTDPGPFWALLDDGTCTPLAKHWVEWGPGCPPCAGTAFHVVPVCF
                     GASNDLNLAIFADGTVPTLTTYGLIIFGVLLAGTLGWVVVRRRKLATAKGSLA"
     gene            complement(5739..6350)
                     /locus_tag="AC16GM_03600"
     CDS             complement(5739..6350)
                     /locus_tag="AC16GM_03600"
                     /inference="COORDINATES: protein motif:HMM:NF039484.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922793.1"
                     /translation="MLIAVFFPDYWYHSFIALIPILGKIVNAAPRAAFRLGFFFGATF
                     FSVSLAESFLSAPFLTLVQLAFGVTLFAVFGWGVGWIKERWGFNPLFIAALWVGFELA
                     LMKMGFARGLLDAPVLALASPFFHGLAILFGSLVISFVVVLFNSLLVLAVEKVLLLIK
                     ARPAVVPESDEIWSFSPGHSFLAQKVYLVPAGRAPPVFPILGV"
     gene            complement(6886..8208)
                     /locus_tag="AC16GM_03605"
     CDS             complement(6886..8208)
                     /locus_tag="AC16GM_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2922794.1"
                     /translation="MRVNFFLKVFGTIFLLLFNSALRAQETSFSTTHNYELPGPFGVA
                     VADLTGDGQVDIAVSTTSGAIGLVNFGQQQFVPALTFSGTNLGNLAVADLDGDGRLDL
                     VAPDRNESVIVFLATGPGQFAPGVAHAVGSVPTQVAASDIDGDTYPDLIATDFGDNTI
                     SILKDYGDAVYGPPHVFAAGQKPYALVATDFDGDGRPDIAVTNQGSNTVSIFTNVGGY
                     AWFAPAVAYAVGLGPAGLAAADFNGDGAPDLAIANSASDDIALLLNNGDGTFAPARYF
                     PAGDSTWALTAVDLDRDGDFDLVAGSLGSHELGLFSNDGRANFQLRGTLPAGTSPSAV
                     AAADLDGNGAIDLVSVDGTSGILTFFGQAPCAVASPGDLNGDGQANVLDVIALIEFVV
                     FRTPALVPGTYAGDLNCDGVVELTDILGLIGNVVFGALVCDPCGSPLR"
     gene            8935..9537
                     /locus_tag="AC16GM_03610"
     CDS             8935..9537
                     /locus_tag="AC16GM_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922795.1"
                     /translation="MKKTRKIAQAALQVGGIILVVGWGLVGCTKKSPTGPVEAPATLA
                     PDKIPSEAASPPPYFQVQPYQDDGTGDSLTFLSAQVSRIVGKGGSKILIPLGQDTSVL
                     QIPHGALTSKVMITIKAQKTLAPDSSWQSTDYVILPAHLTFAKPATLTHQTKGLRNGT
                     TLRLLEYQNPVWNQLGTANIIKTKATFKNLSRAGTYRVQP"
     gene            complement(9575..10048)
                     /locus_tag="AC16GM_03615"
     CDS             complement(9575..10048)
                     /locus_tag="AC16GM_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2922796.1"
                     /translation="MTSNELKHFLEQWEREYVTTVKVLQAIPDDRLDYKPAEKSRSLR
                     DLMWHIVGVENFAATLALTGAYPKERLPPPATVKELLEFYERQHKALVSQVAGAEAAL
                     LSRTVDFFSRPVPVSHILWRVLLYHQIHHRGQLSVYLRLVGAKVPSIYGPSADES"
     gene            complement(10085..>11729)
                     /locus_tag="AC16GM_03620"
     CDS             complement(10085..>11729)
                     /locus_tag="AC16GM_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR2922797.1"
                     /translation="VIVTIPAFLIWQASEPEFTFKIANWQIVVLSILGGFLGILFMIP
                     LRRHLIVKEHGILPYPEGTACAEILVAGDEGGSKARTVFAGLGIGGLYKALMSGGKLW
                     PDNFHFTPGFFKGSQMGVDATPALMGVGFIIGPRIAALMFAGAVLGYVGIGPLLAFLG
                     NYLTTPVPPATVPMAQLSPAELRLYYIRYIGIGAVAMGGFISLLRSFPSIISSFKQGI
                     GDFAARGATATVRTDQDLSLKLVLGGSFLVAVAIWLLPGTQMHFVGALLAVLFGFFFV
                     TVSSRMVGIVGSSSLPVSGMTIGTLIVASVILLAYGVSGTAGMVGAMMVGTVVAIAVS
                     MSGDASQDLKTGFLVGATPKKQQIAEFVGVLVPAVIMGYVLLFLNKTHGFVGPGAFEA
                     PQANAMSAVVKGVMTGNLPWIFVIVGTMVGLSIELLGVHALPVAIGLYLSPDLSTPIV
                     IGSLIYLAVQTFSNGGLKNSRIEKGILFGSGLVAGDALVGVGIAFLVGSISSYEAFFR
                     REDNIGTWLGGYGQIFTLGIFLLLGLGYLYHIWRMKARA"
BASE COUNT         2711 a         3415 c         3335 g         2268 t
ORIGIN      
        1 atccgttcgt gtttttcggc ggcgcaagag aaaaatactt ccggtaggct acgcaaccta
       61 atatcggcca tttgcgcggg cttctttatc tccggccttc cgatgcccca cagccatttg
      121 cttttccatc ctcagccgtt ttacttggcc gaccaatgtt cgcttggttc ggaacttctt
      181 tcccctcgcg ctgcggcacg actttttttt tggccggttt tctcggcctg acggccttcg
      241 cttcgcccgc ctcccgcgct gctgatccgc ccgcagaact gaaactgcct ccccacaccg
      301 ccgaaattct ggcgggcatc gacctctcca cccgggagga ataccagaaa gcccgggccg
      361 tcttccgctc gctcgtcgaa cgcgattcca ccgatccctg gggctggttt ttcctggcgg
      421 cgggcctcca agacgagatg atggacaaag aaaatttcat ctggaaggag gaattcaaac
      481 aggccgtcga gcgaaccctg cttttagcca aagcgcgggc cgaccgcgac aaaaaagacg
      541 cctggagtct tttcattctg ggaaacgcct acggctacct gaccgtcttt ttggcccggc
      601 agggcgattg gttttcgggt atgcgcgcgg cggtgaaggc caaagggtac tatgaaaaag
      661 ccctgaaaat caacccgcag ctctacgacg cctacgtcgg tttaggaaac taccactact
      721 ggaagagcgt gcggacgaaa gcttttctct ggctgccttt tttcaaagat acgcgcaaac
      781 agggaattgc cgaattggaa aaagcggcgc ggctggggac ctttgcccgg accgccgcct
      841 gcttctcctt gatgtacatt tacattcacg agaaacgcta tgacgcggcg ctggccttag
      901 ccgacagtct ttcccgcgag tacccggaga gcaggagttt cctctggggc caggccgccg
      961 cgtacttcga aaactaccat tgggccgagg ccgcgcgggc ctacgccgag ctggtgcacg
     1021 agatcgaaga ggaaccggac cagaactact acaacctgat cgactgcacc taccggatcg
     1081 cccagtgccg gttcaacctg gggcggttca acgactgcat tacctcctgc gaaaaacttt
     1141 tggcctaccc ggcttctccg gagatagtca aaagacagga agcgaaaatt cgcaagacgc
     1201 gcgcgctttt ggagaaagcg cagaaaatca ccggaaagaa gaagtagact ttaggccgcc
     1261 agctcgggcc gctcgcccgc agagggaaaa ggaggcgcaa tctcccggga aattgcgggt
     1321 gccggcaatc tctctgaggt tgggcgatca gcgtatcgta agtagagggc ggtttggcat
     1381 gagtaaaatc ccaaccgcag ccagatgcgg ctcaaaaggc acagctgctg gaggatgagc
     1441 accagccaga aaatgcccca tttctcggtc ggtgcattta tctcaatcaa gaagtaaatc
     1501 aggaaaaaga cgagaccaat agccaccaag gagaagtaca gccggaccgt ccggccaaag
     1561 ttacggaaga cgaagcgaaa cgaccggcgc aaggcggcaa acgtcttccg ggaatcttcc
     1621 accaccagcc ctatcttggc gtagtcgaag atcatcgaaa agaagcacca gacgccgaag
     1681 gagagcaccc aggccacccc gtacgcccag atggtgaggt gttcgttgcc tctgttcgcc
     1741 gaaaccagac cggcgatttt gctcatgatt ttggtgatga tgtagaatac gattacggcc
     1801 ggcaaaaacc agaggaaaag ccgcaggagc ctcccccagt actttccgca attagccaga
     1861 aacgaagaaa gaaggggcct gttctgatcg ttgttgaact gggctataat gccgccgtca
     1921 aaaaaggtgt aaaggaaaag gaagaacaag agtgcggggc caagcaggcc gaaggcggcg
     1981 tcgagcgccg cgccccggtg ttcgatgaaa tcgctcataa gcgcaaaatt aaaaccctcc
     2041 cccagtgtcc gccccgccaa acttgtcccc gcggcatcgg caaatgccgc ccgcaggggg
     2101 taggtgagcg ccgcggcgca aaccaaattc gccagccaga gaaaaaacag aaggcgctgg
     2161 gccgcgagcg cccggcccaa gccggggaaa agaaatctcg gctttgtgtt catctagaac
     2221 accgccaagg tttgcaacaa attctgcatc acaaaaagcc accgggagga ccaccgggtg
     2281 atcgcccgcg actgtttcac tatcgtccga ctgttgttga gccgattaag atcgaggacg
     2341 attttattct gcggatcaac cgccgcatac tccagttcgt gcggcccccg gaaacggtac
     2401 ttaatccagc gctcttcccc atcccagtgg taggtgaacg gcttttcctt ctcgaacttg
     2461 atttcaatct ccaccggaaa gataccatcc cctttccgct ggatctgaac tgctgactcg
     2521 tagactttta cttctttttt cttgccgttc ctgtccttcg ctttcgcaag cgtcttgcct
     2581 ttggtcagag cggtgtcgcc caatacggtg gtatccccgc cgctttccac gaacaccttc
     2641 cggccgtcga ggtcaaaaaa gccccgctgg ggcttgattt cttggctcgc aatgccggcc
     2701 acggcgtaat ccaccttctg ggtaccgtag acgaactggt tgaagaacca atccagattt
     2761 tgcccggaaa cttcgttggc cgtgcgaacg aaatcctccg tggcgggatg cttgaaccgg
     2821 aagcgttcga agtaggtgcg catcacattc tccatccggt cggggccaag gtagttctcc
     2881 agggtcgtga ggagtatctg aaccttggca taggagttag tgccgtaact attaccgttg
     2941 aaaaaccgcc aggaaaaggt attgaccggg tcgtagtgcg ggtactgcac cacctgccag
     3001 cgggcgaatt ctaattcgct gatcgcagca cccaggcagc ggccaaaact cgccttatcg
     3061 ccgaaccagg ccgccagcac cttgccggtg gagtaggtgt tgagcccttc atccaaccac
     3121 gattcttcga actcgttgga gccgaccagc ccgtaccagt actggtggcc gaattcatgg
     3181 acgatggtct cctccacgag gtagtgaatg ttgcgcaaaa gcggccagtc ggccgggacg
     3241 agcgttatgc cggtgaaaag cgtgggatac tccatcccgc cggcgccgaa agcccccgtg
     3301 ggcggcatca cgatggttaa agtcgcgtag ggatactccc cgtaccaccg gccaaaatat
     3361 ttcagcgcct ggtgggccgc ctccaggagc ctttgctttt gacccgcata atcgggttgg
     3421 ttcaggagcg tgatcgccac tctatgtccg ctcgggaaaa cgtgcgtatc tggctgcacc
     3481 acaaaggccg gtgaagcggt ccaggcaaag tcgtgaacat cttcctgcaa gtaggtgacc
     3541 gtttgcgtgt gcgctttcgg gtctgccgac tcactggtgc gggcgccggt ggccccgact
     3601 ttgaagccgt cgggcacggt cagggtcacc cggtacgagc cgtagtcggc gtagaattcg
     3661 ctgttggcgt ggaactgatg gcaattccag ccgccggttt tgcgtccgcg catcccggct
     3721 ttttcgtata ctcccaattt cgggaaccac tgtcccacca tgtagaagtt gcgcttgtag
     3781 ccggtgcggg cgaagacttc gggcagtttg gagaaaaatt caatccttag cgtcacctgc
     3841 ccgccgggcg gcaccggctt cggcagtttt acggaacaga cggtctggtc gtccagattg
     3901 ttgtcatccg ggtgttcgta gcgcagcgtg ggcaacaaat cggtgccgtc ggctaaagtc
     3961 attttctgaa tgtcgatgta cccccacttg tcaccccgca acttcccccg gcgccggcgg
     4021 ccgcctagtt ccttgaagaa agtcgacttt tcgtttttga atgcgttcag gtaaagatgg
     4081 aaccggagac tggagacgtg atcaggcgaa tcgttaatcc aggtcacggt ctcctgcccg
     4141 atgaccgctt tcggaaaggt atccagtttg gcggtgatct cgtagtggac cacctgacgg
     4201 gagagggcag ccctggcggg aagcggaaag aaaattccgg cgagaaacag accgaacaaa
     4261 aacgctctca gcgtgttttt acgtatcggc acggggacgg agcatatgcg aaagacggcg
     4321 attggtcaat cgacttcagg ggtggcttgc ctccgtcgat atgctaagcg agaggagatc
     4381 cgctgccgcc atgacccgga ataagagaag aaccgccgcc tccctgctgt tgctggtgtt
     4441 actcctcgtt ctctgggtgg tgttcaaccc cttcggacgg tttggctggt gctccttcgg
     4501 gttcacgatc tacaatgcca ttccccgtcc ggtgaccgat atccaggtgc gcgccgacgg
     4561 gaaggtgaga cgggtcgcca agacgcacga cttacggtat gaagatattg tgtggctgct
     4621 cgacccgaaa cccgatctcc tcatcatcgc caccggctgg gatggcgtca ccgtcccgcg
     4681 cccggaaatc ggaaacctcc gggattgtga ggtgcggatc ctgaaaacgg aagaggcgcg
     4741 gcttctcttt aacgcgctca agcggtcggg gaagcaggtg gcgatccact tccattcaac
     4801 ctgttaggct gaaacaaaaa gagaaagagc ggaatgctcc gctctttctc ttaaactact
     4861 tctgactgct cttttcccta cgccaaactg ccttttgccg tcgccagctt cctccggcga
     4921 acaacgaccc atcccagcgt gccggctaag agcacaccga agatgatgag accataagtg
     4981 gtgagcgtcg gaaccgtgcc atcggcaaag attgcgagat tgaggtcatt gctggcaccg
     5041 aagcatacgg gaaccacgtg gaaggctgtt ccggcgcagg ggggacagcc aggcccccat
     5101 tcgacccagt gttttgccag aggggtgcag gttccatcgt ccaggagcgc ccagaaggga
     5161 ccgggatcgg ttggcaaatt cataagcgta atgtggaatt tctggcctga gtagaagtgg
     5221 agatttttgg acgtgaaatc ggatgcgaca gactgaacac cgttcggtgg ccagggaccg
     5281 ggggccagtt gctcggcggg ggtaatcgtg tcgcaggcca agagtcgggt agtatccgga
     5341 acacccggcg ggcaggaagg gctgtctccc cagacgtata ccaccacgtt gttcacggta
     5401 tcattcgtcg agtcagggcg gttatagagg agaacgtcca ccgttcccaa cgtgaaggtt
     5461 ttgccggcgg gaacgtcaaa gcgcatgccc tcgtacgtgt ctccgacgcc atccggcacc
     5521 gggtagatga agctcaagtt gcaggcgtac cccaggctca agggaaaggc cgtcagggta
     5581 cgaccgtgct ctattttcgc tggcaactgc tcaagcttgg actggtcgac ggccagagca
     5641 gtggcggcca cgaccaacga caagatgaac actgcgacaa ttgttttgcg caacactttt
     5701 tcctccattt cggttaaagg ttcattcgca cgtcgctatc aaacaccgag aatggggaaa
     5761 accggggggg ctcggcctgc cggaacgagg taaacttttt gagccaaaaa cgagtggccg
     5821 ggtgaaaaac tccaaatttc atcgctctcc ggaacgacag ccgggcgggc tttgatgagc
     5881 aggaggactt tctcgaccgc cagaaccaaa agcgaattga acaacacgac gacaaacgaa
     5941 atgaccagag aaccgaagag gattgccagc ccgtgaaaga agggggaagc gagggccaaa
     6001 acgggagcat ccaggaggcc gcgggcaaac cccatcttca tcaacgccag ttcaaaaccg
     6061 acccatagcg cggcgatgaa aagcggatta aaaccccacc gctctttgat ccagccgact
     6121 ccccagccga aaaccgcaaa cagggtcacg ccgaaggcca gctggacgag cgtaagaaaa
     6181 ggcgcagata aaaacgattc ggccagtgac acggagaaaa aggtcgcgcc aaagaagaac
     6241 cccagccgaa aagcggcccg gggggcagcg ttgacgattt tcccgaggat gggaattaaa
     6301 gcaatgaaag agtgatacca gtagtcgggg aaaaaaaccg cgatgagcaa caggaaggcc
     6361 gatgttccgg cgcagaggaa actttccgcc cacccgggag tgcctcggcc gggggggcgt
     6421 atgcattcag cttcacgccg cgcacgaccg ttcagcaagc ttcgtctggt tgttccccgc
     6481 tgctctgcga tgtgccctcc cagcccaacc gctgcgtcgc agtggtcagg acagccggct
     6541 attcacctga tgaacttcgt ctgatgcctg cggcctgctg tccttagcaa tgccaaactc
     6601 aagtcatcaa gccgttccgc aaattcgccg ggatgctccg gcgatttcct ctcactttgt
     6661 ggttgcgggg tcttctgcca gtaaactgaa acatccctgc ctgacaccca acctaaagta
     6721 cccgcacttt atatttaata tatctcattg taatctgcag tcaagtgatt tcttcaaaaa
     6781 acgaccgcgg ttaagtgact atgggacaag ggaatccaaa gacaagtccg gccgactgat
     6841 ggttgaatgg gttcagaaga tgacgtgaac cgaccgcttg ggtgcctacc ggagcgggga
     6901 gccgcacgga tcgcagacca gcgcgccgaa aacgacgttg ccgatcaaac cgaggatatc
     6961 cgtcaactct accacaccgt cgcagttcag atcgcccgca tacgttccgg gcacgagcgc
     7021 tggagtgcgg aaaaccacga actcgatgag cgcgatcaca tccaaaacgt tggcctggcc
     7081 gtcgccgttc aggtctccgg gacttgccac ggcacaaggc gcctggccga agaaagtcaa
     7141 aataccgctg gtgccatcta ccgacactaa gtcgatcgcc ccgttcccgt ccaggtcggc
     7201 ggcggccacg gcgctggggc tcgtgccggc ggggagcgtt ccgcgcaact ggaaattggc
     7261 ccggccatcg ttggagaaaa gccccagttc gtggctgcct agagaaccgg ccaccagatc
     7321 gaagtcgccg tcccggtcga gatcgactgc cgtcagggcc caggtgctgt ccccggcggg
     7381 gaaataccgg gccggggcaa aggtgccgtc cccgttattg agcaagagcg caatgtcatc
     7441 gctggcgctg ttggctatgg ccagatcggg tgcgccgtcg ccgttgaaat cggcggccgc
     7501 caaaccggcc ggcccaaggc cgacggcgta agcaaccgcc ggagcaaacc atgcgtaccc
     7561 gccgacattg gtgaaaattg agacggtatt tgagccctgg ttggtcaccg cgatgtcggg
     7621 gcgcccgtcg ccgtcaaaat cagtcgcaac cagagcgtaa ggtttttgcc ccgcggcaaa
     7681 cacgtggggc gggccgtaga ccgcatcgcc gtagtctttc agaatcgaga tcgtattgtc
     7741 gccaaagtcg gtggcaatca agtcgggata ggtatccccg tcgatatcgg atgccgccac
     7801 ctgggtcggc accgacccca cggcatgcgc cacgcccggg gcaaactggc ccggtccggt
     7861 agcgaggaaa acgatgaccg attcgttccg gtcgggagcc accagatcga gacgcccgtc
     7921 gccgtccaaa tccgccaccg caagattccc gaggtttgtt ccggagaagg tgagtgcggg
     7981 aacgaactgc tgctggccga aatttaccag accgattgcc cccgaggtcg tggagacggc
     8041 gatgtccacc tgcccgtcac cggtcaaatc cgctaccgcc acgccgaaag ggccgggaag
     8101 ctcgtagtta tgggtggtgg aaaaactggt ctcctgcgcc cggagtgcgg agttgaacaa
     8161 gaggaggaaa atcgtcccga atactttcaa aaaaaagttt accctcatca cctcaccacc
     8221 tttccccgcg cgggcttgtc cgttgctcca aaggagtccc actgcaccgg aggcaaaccg
     8281 gccgcacggc acttccgcca tcgctgtcta atatgcgctg cggggcgcgt caactggttt
     8341 ctctaaaaaa ccgggagggt aaccctctcg gaaggagtcg taccgacccg caggaactgc
     8401 tttgccggag ggctacttca tcttcttcat tggtttgccg caacacatca tcggcttgga
     8461 agatttgtag tcacagacct ggcattggta ccgggctttg gtcgatttct tcgctttctt
     8521 cgcgggcata cttttcacct cctcagactt agccactgag gccgttctct acaataatcg
     8581 gaaaccaaaa tagctcataa tggtcaattt gtcaaacacc cccgggggta gaaataaccc
     8641 ggttcaactt ttgaggagag tccgccgccg gcccggctgg catctctggc atctaaagta
     8701 agggcgctca agatcaaatc tttttctctg ggtggcacca gtcaagccaa gaggggctgt
     8761 tggcgaaatg ccataaagta tgggattgtc cagattctta gcagcgattc aattattgca
     8821 caattccaat ccatccccgt aatctcttgc tccgaaagca gtagcgcaat tttcctgcgg
     8881 tccgtccggt tggcattgcg ttcgcagtga tcagtaggtg aaatgaaaga gcaaatgaaa
     8941 aaaacaagga aaatcgccca agcggctttg caggtgggcg gcatcatttt ggtggtcggt
     9001 tggggtctgg ttggctgcac caagaaatca cccacggggc cggtcgaagc cccggcgact
     9061 cttgctcctg acaagattcc ttcggaagcc gcctcgccgc cgccctattt tcaggtgcaa
     9121 ccctaccaag acgatggcac cggagatagt ttgacctttc tgtccgctca ggtgagccgc
     9181 atcgtcggga aaggcggtag taaaattctc atccctctgg gccaggacac gagcgtttta
     9241 caaattccac acggcgccct caccagcaag gtgatgataa cgatcaaagc gcagaagaca
     9301 ctggcccctg atagttcctg gcagtcgacc gactacgtga ttctcccggc gcacctcacc
     9361 tttgccaagc ctgccacgct cacgcaccaa acgaagggac tccggaatgg aaccaccctc
     9421 cgcctcttgg agtaccagaa ccctgtctgg aatcagttgg gaacggcgaa catcatcaaa
     9481 acgaaagcga ccttcaaaaa tctttcccgt gccggcacgt accgggttca gccgtaagtc
     9541 tccctcataa tctccttggt ttaatagcct tcttctacga ttcgtcagcc gacgggccgt
     9601 aaattgaagg tacttttgcg ccgaccagcc gcaggtaaac cgagagctgt ccccggtggt
     9661 ggatctggtg gtagagcagg acccgccaga gaatgtggga aaccggcact ggccgggaga
     9721 aaaagtccac ggtcctactc aggagcgcgg cctcagcacc agcgacttgc gacaccaacg
     9781 ccttgtgctg ccgttcataa aattcaagga gttctttaac ggtggccggg ggaggcagtc
     9841 gctctttcgg gtaagcgccg gtcaaagcca aggtggcggc aaaattttcc acgcccacga
     9901 tgtgccacat caggtcgcgg agcgagcgac ttttctcagc cggtttatag tccagtcggt
     9961 cgtccgggat cgcctgcagc accttaacgg ttgtaacgta ctcccgctcc cattgctcca
    10021 ggaagtgttt taactcgttg gaggtcatct ttcctcttag gtacccgaaa cctgctgcgt
    10081 tcggttacgc gcgcgctttc atccgccaga tgtggtacaa atatcccagc cccaagagca
    10141 ggaaaatacc cagggtgaag atctgcccgt agccgcccag ccaggtgccg atgttatcct
    10201 cacggcggaa gaacgcttcg taggaagaga tcgatcccac cagaaaagcg attcccacgc
    10261 cgaccaaggc atctccggcg accagccccg agccgaagag aattcctttt tcaatgcggc
    10321 tgtttttcag accgccattg gagaaggttt gcaccgcaag gtaaatcaga ctgccgatga
    10381 cgataggcgt cgaaaggtcg ggtgaaaggt ataaaccgat ggccaccggc agggcgtgca
    10441 cgcccaaaag ttcgatggag aggcccacca tcgtcccgac gatgacgaaa atccagggga
    10501 ggttgccggt cattacgccc ttgaccaccg ccgacatcgc gttggcctgc ggcgcttcga
    10561 aggcccccgg ccccacaaag ccgtgcgttt tgttcaagaa tagcaggacg taccccatga
    10621 taaccgccgg caccaaaacg ccgacaaatt cggcgatctg ctgctttttg ggcgtggctc
    10681 ccacgagaaa accggttttc aaatcctgag acgcgtcgcc cgacatggag accgcaattg
    10741 ccaccaccgt ccccaccatc atcgctccca ccatgccggc cgtgccggag acgccgtacg
    10801 ccaaaaggat aaccgaggcg acgatgagcg tgccgatggt catgccggaa accggcagag
    10861 aggatgagcc gacgatgccc accatccggg aggaaaccgt cacgaaaaaa aacccgaaca
    10921 agaccgccag aagcgccccg acgaagtgca tctgcgtgcc cggcaaaagc cagatcgcca
    10981 cggctaccaa gaatgacccg cccaggacca atttcaaaga caggtcttga tcggtgcgca
    11041 cggtagcggt tgcccctcgg gccgcaaagt caccgattcc ctgcttgaac gaggagatga
    11101 tggaggggaa cgaccgcaaa agcgaaatga agccacccat ggccacggcc ccgatgccga
    11161 tgtagcggat gtagtagagc cgcagttccg ccggtgagag ctgagccatg ggaactgtgg
    11221 ccggcggcac cggcgtggtg agatagtttc cgagaaaggc taaaagcggc ccgatgccga
    11281 cgtagcccag aacggcgccg gcgaacatca aggcggcaat ccgcggcccg atgatgaacc
    11341 ccacccccat caaagcgggc gtcgcgtcaa cgcccatttg cgaccccttg aagaacccgg
    11401 gcgtgaagtg aaaattgtcc ggccagagtt tgccacccga catcagggct ttgtacagac
    11461 cgccgattcc caggccggcg aacacggtcc gcgctttgga accgccttcg tcaccggcga
    11521 ctaatatctc cgcgcaggcg gtgccctcgg ggtagggcag gataccgtgc tctttgacga
    11581 tcaaatgccg gcgaagcgga atcatgaaga gaatgcccag aaaaccgccc agaatcgaca
    11641 aaaccacaat ttgccagttg gcaattttga aagtgaactc cggctcggag gcctgccaga
    11701 tgaggaaggc ggggatcgtg acaatcact
//
LOCUS       DBNGZD010000131         2832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1897493, whole genome shotgun sequence.
ACCESSION   DBNGZD010000131 DBNGZD010000000
VERSION     DBNGZD010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1897493"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            840..1967
                     /locus_tag="AC16GM_03625"
     CDS             840..1967
                     /locus_tag="AC16GM_03625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR2922798.1"
                     /translation="MAKILHTADCKLGSAFPDLGFAGEKVREIQKATFKRIIDLALDE
                     KVDLFLVAGDLFASNLVSKGLVEFVLDEVGRLGEIPAVVLPGDCDAWGPDSVYHWLPF
                     LEVPKNFYLVTLANQPLFFEKIDVSVSGIPVTSSAADESTWKELKKPEEEGHHILLVH
                     GQPVGGKDSDAQPPFVRETAVEKAGFDYVALGHQPAWAEKSLDKGKAVNPGYPEPLDF
                     EQEASGKVAVVKISRSRLDISSETTGSLTWVRQTVPASKLKTAAEIHEMTKKHKGEKN
                     LCEVVLEGEAEHWLDLEKLYDDLAPRFLSLRIDDRRTYPDLRRGSREIVETTILGQYR
                     KFLQGQWDGAEGGDHQLVAAAWTLGQTLLSKDISESAADAD"
     gene            1957..>2832
                     /locus_tag="AC16GM_03630"
     CDS             1957..>2832
                     /locus_tag="AC16GM_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2922799.1"
                     /translation="MQIERCQIKAFGKLKQFTGEFGPGVTVIQGPNESGKSTLVQALC
                     AAFFDSAAGDPGEKEKQGTLVPKNLGEVTLDFKTDGAHYRLHRDFSDGAVKLEKKPGG
                     LTLKDPKGVAEILAGELGLESPELFFATACLRQDESSPTPAASEAVRGKLKEVVTGGP
                     QARAAEAAAQLLKREGEFLQSPDSPDSLPTLEKKQEFLKTEITRLTEELETLRQSRVD
                     FAQIDATLQQVSFSAKEKEAELVTARRLYEAEQALGALAKEFQDLTLRIRELQKSEQV
                     VTTLRATLSGLTPVER"
BASE COUNT          663 a          762 c          848 g          559 t
ORIGIN      
        1 caatcttggg gcccgtgaag atcaaccaaa ggtctgacat ccaatctgaa aattcggaaa
       61 agtgaaagga ggtggtcgag cgaagcaccc cacgcagcaa aggaacgcag cggatttaac
      121 gaaaaaccta aaatctgcac agggaggtga atgacgatgc agaaacaaaa aatcctcacc
      181 gtctcgacca tatttgttct ggtcttcttc gtctccttcc taacagccaa acaagttagt
      241 caggcgaaga tgggtgggaa ggccttcgtc aaaacgtggt tatttaacga cgtatgccct
      301 ttacctggcg ggtactggcc gggagaaacg tagaggttgc gcacgtgctc ggctcatcat
      361 acactaaggt gtgggtaaac gggcaggttg tctacagcac gtacccaaag aatttcggct
      421 cttccaccct gacggcagtg ggcggcggtg gtactccgca gtgcgtgatt gtgcaacatc
      481 acgggataat ctgcggcacc atagactgtt ggcctcagga atgttgtgaa ttaacgtggg
      541 tggagtgtga agcgggctca ggaggaaacg cctgtacctg cggtagtacg taccctcctt
      601 accagcaata acactggtgg gctctaagcg gagggatcac cgaagtgatc cctccgcttt
      661 ttattactcc ttgtttacac aaggaatagt cgagctggct ttaccacgcg actaccccac
      721 cgcctgacct gctggggttt acctcgtaac ctaaatatcc ccttccggtt tgccgataca
      781 taagtagcgt caattcgggg cacagaagac ggttttgtgc cttttgtttc cggatgacta
      841 tggccaaaat tctgcacacc gccgattgca aattaggtag cgctttcccc gacttgggct
      901 ttgccgggga gaaagtgcgg gaaatccaga aggccacttt caaaagaatc atcgacttag
      961 ctctggatga aaaagtcgac ctcttcttgg tggcgggcga tctgttcgcc tccaatttag
     1021 tttccaaagg tctggtcgag tttgtcctcg atgaagtagg gcggttggga gagattcccg
     1081 cagtcgtttt gcccggcgat tgtgacgcct gggggccgga ttcggtttac cactggctgc
     1141 cgtttctgga ggtgccgaaa aacttctacc ttgtgactct ggcgaaccag ccgctttttt
     1201 ttgaaaaaat cgacgtttca gtttccggga ttccggtcac cagcagcgcc gccgacgaat
     1261 cgacctggaa agaactcaag aaacccgagg aggaaggcca ccacattttg ctcgtgcatg
     1321 gtcagcccgt cgggggtaaa gattccgacg cccagccgcc tttcgtccgc gagacggcag
     1381 tggagaaggc cggattcgac tacgtggctt tggggcacca gccggcctgg gcggagaaaa
     1441 gtctggacaa agggaaggcg gtgaatccgg gatacccgga accgctcgat tttgagcaag
     1501 aggcttcggg caaagtggcg gtcgtcaaaa tcagccgaag ccggctggat atttcctccg
     1561 aaacgaccgg ttccctcacc tgggtccggc agaccgtgcc cgccagtaag ttgaaaaccg
     1621 cggcagaaat tcacgagatg accaagaaac ataaggggga gaaaaatctc tgcgaggtgg
     1681 tcttggaagg cgaggcggaa cactggctcg acctggagaa attgtacgac gatttggcgc
     1741 cgcgcttcct ctccctgcgg atcgacgacc ggcgcaccta tcccgacctc cgccgcggat
     1801 cccgggagat cgtcgaaacg accattctgg gacagtaccg gaaatttctt caagggcagt
     1861 gggatggagc ggaaggcggg gaccaccagc tggtcgccgc cgcctggacg ttggggcaga
     1921 cgctgctctc aaaagacata agcgaatcgg cggccgatgc agattgagcg ttgccagatc
     1981 aaagcgttcg gtaaactgaa gcagtttacc ggcgagttcg ggccgggggt gacggtcatc
     2041 cagggtccca acgagtcggg caaatcgact ttggtgcagg cgctctgcgc ggcttttttt
     2101 gattccgcgg ccggggaccc cggtgagaaa gaaaagcaag gcactctggt cccgaaaaat
     2161 ttgggagagg taaccttgga tttcaaaacc gacggggcgc actaccgtct gcaccgggat
     2221 ttttccgacg gcgcagtgaa attggaaaag aaaccgggtg gcctgacgct caaggacccc
     2281 aagggggtcg ccgagatttt agccggcgag ctgggtctgg aaagcccgga gctctttttt
     2341 gccacggcct gtctgcgtca ggacgaatct tcccccactc ccgccgcgag cgaagcggtg
     2401 cgcggcaaac tcaaggaagt ggtcaccggc ggaccgcagg cccgcgcggc cgaagcggcg
     2461 gcccagttgc tcaagcggga aggggagttt ctccagagcc cggacagccc ggacagctta
     2521 cccaccctgg aaaaaaagca ggaattcctg aagacagaga tcacccgcct gaccgaagaa
     2581 ttggagacgc tgcgccagag ccgcgtcgac tttgcccaaa ttgacgccac cctgcaacag
     2641 gtgtcgtttt ccgcaaaaga aaaagaggcg gaactggtca ccgcccgccg tctctatgag
     2701 gccgaacagg cgctgggagc gctggcaaag gagttccagg acctcaccct gcgcatccgg
     2761 gagctgcaga aatccgaaca ggtcgttacc accctgcggg ccacactgtc gggattgacg
     2821 ccggtcgagc gg
//
LOCUS       DBNGZD010000132         3058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_646497, whole genome shotgun sequence.
ACCESSION   DBNGZD010000132 DBNGZD010000000
VERSION     DBNGZD010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_646497"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="AC16GM_03635"
     CDS             <1..1480
                     /locus_tag="AC16GM_03635"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HIR2922800.1"
                     /translation="DAFKEQAEGLLEGGVDLFIVETFSDLTEITQAVKAVRSVCDLPI
                     VAQMSFTEEGATLAGQSPEAVAQALEALGVDMLGTNCSVGPQALLDVLLAMRRTTKRP
                     LSIQPNAGLPRLVEGRWFYYTSPTYFAEYAQKFLEAGATLVGGCCGTTPEHIHAMAQS
                     LKRFRATATEVHPAPEAPRVQVVEKPEPLPAFEASHFAQKLGKKFVVSVELDPPRGIN
                     PEKLLKGAELMEKHGVDAINIGDSPMARVRMSCIAIGVLIQKRVKNIDIILHWTTRDR
                     NLMGLQSDLIGAHALGIRNILAITGDPPTIGDYPHATGVYDVDSIGLIQVIARLNTGV
                     DWAGNSIGSPTALTIGCGVNPTAEDMAKEVERYKKKIEGGAQFAFTQPLYELSILEEF
                     LDRTHDCRIPVLLGLLPLQSTRHTEFLHNEVPGITIPADIRKRMAEAGHNGAQEGIAM
                     CRDLLEKAKDMVSGAYLMPSFGRYETVLEVVKGFLTKTQVVT"
     gene            1594..>3058
                     /locus_tag="AC16GM_03640"
     CDS             1594..>3058
                     /locus_tag="AC16GM_03640"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR2922801.1"
                     /translation="MEIQVLKGEIQDAKDEAIVVNLFEGVKAPGGATAAVDQKINKSI
                     TKLIATGDFTGKKNQVAVLYPPDGFPAKRVFVVGLGKKEKFNLDVIRQAAGSVCQKAQ
                     QLNVKSYTTIVHGAGAGRISPDDAAQALVEGTLLANYRFDRYHTETDSRPQPVQKVTL
                     LERDGRNIVRMKKGAAIGEIISWAQCLARDLSNEPGNVLTPTKLAETAADLGKKYGFK
                     TQILRKAEIEKLKFGALLGVARGSAEPPTFTVMEYQPSKRAFGPIVLVGKGVTFDSGG
                     ISIKPADNMDEMKGDMTGGAVVIATITAAARLQLPVHLIGLVPSTENLPSGTSYKPGD
                     VLRSYLGKTIEIINTDAEGRLILADALAYAQTFKPKAVIDVATLTGAVKIALGTVAAG
                     LLASDQKLANRLKAAADKSAEKVWQLPLWEEYQERIKSDIADVKNSGGRPGSTITAAC
                     FLQKFVGDSPWAHLDIAAMDIEEKGQPYVPKGGTGFGC"
BASE COUNT          688 a          907 c          871 g          592 t
ORIGIN      
        1 tgacgccttc aaagaacagg cggaaggatt gctcgaaggc ggggttgacc tcttcatcgt
       61 tgaaaccttc tccgacctca ccgagatcac gcaagccgtg aaagctgtcc gttccgtttg
      121 cgacctgccc atcgtggcgc agatgagctt taccgaggaa ggcgcgacgc tcgcggggca
      181 gtcgcccgaa gcggtggcgc aggcgttaga ggccctcggc gttgatatgc tcggcaccaa
      241 ctgcagcgtc ggcccgcagg ccttgctgga tgtgcttctg gcgatgcgac ggacgacgaa
      301 gaggccattg tcgattcagc ccaacgccgg tcttccccga ttagttgaag gacgctggtt
      361 ctactacacc tcgcccacct acttcgccga gtatgctcaa aaatttttgg aagccggcgc
      421 gacgcttgtc ggcggctgtt gcggcaccac gccggagcac atccacgcca tggcgcaaag
      481 tctcaagcgc ttccgggcta ctgcgacgga agtccatccg gctccagaag cgccccgcgt
      541 tcaggtcgtc gaaaaaccgg agccgctgcc cgcatttgag gcctcccact tcgcgcagaa
      601 gttgggtaaa aagttcgtcg tttcggtgga actcgatccc ccgcgcggca tcaatccgga
      661 gaaactgctc aagggcgccg aactgatgga aaaacacggc gttgatgcca tcaatatcgg
      721 cgactcccct atggcccgcg tgcggatgtc ctgcatcgcc atcggggtct tgattcaaaa
      781 gcgcgtgaaa aacatcgaca tcatcctcca ctggacgacg cgagaccgca acctgatggg
      841 cctgcagtcc gacctgatcg gcgcgcacgc tctggggatt cggaacatct tggccatcac
      901 cggcgaccct cccaccatcg gcgactaccc gcatgccacg ggagtttacg acgtagattc
      961 catcggcttg attcaggtga tcgcccggct caacaccggc gtcgactggg ccggcaactc
     1021 catcggcagc cccaccgctt tgaccattgg ctgcggagtc aatcccaccg ccgaggatat
     1081 ggctaaggaa gtggagcggt acaagaagaa aatcgagggc ggcgcgcagt tcgctttcac
     1141 ccagcccttg tacgaacttt ctattctgga ggaattcctc gaccgcaccc acgattgccg
     1201 gattccggtg cttttaggtc ttttgccctt gcagagcacc cgccatactg agtttttgca
     1261 caacgaggtg ccggggatta cgattccggc tgatattcgc aagcgcatgg ccgaagccgg
     1321 ccacaacggc gcgcaggaag ggattgcgat gtgccgcgac cttctggaaa aggcgaagga
     1381 tatggtttcc ggtgcttacc tgatgccttc cttcgggcgc tacgagacgg tgttagaagt
     1441 ggtgaaaggg tttttgacca aaacgcaggt ggtaacgtaa aaacaatcgt gcctcgggag
     1501 gcgcggcttt agccgcgcat gacgacccat ggcgtgcgac tgaagtcgca cctcccggta
     1561 ttgggtccca gaggtagatt gcaaggagca aacatggaaa tccaagtgtt gaaaggtgaa
     1621 attcaagacg ctaaagacga agccatcgtc gtcaacctct ttgagggggt taaagccccc
     1681 ggcggggcca ctgcggcggt cgatcagaag atcaacaagt ccataacgaa actgatcgcc
     1741 accggcgatt tcaccggcaa gaaaaatcag gtcgccgtcc tctaccctcc cgatggtttt
     1801 ccggccaagc gagtcttcgt cgttggcctg ggaaagaaag aaaaatttaa tctcgacgtc
     1861 atccgccagg ccgccggttc ggtctgccag aaagcgcagc agttgaatgt gaaatcgtat
     1921 acgaccattg tgcacggcgc cggcgcggga cgaatcagcc cggatgatgc cgcccaagcc
     1981 ctcgtcgaag gcacgctttt ggccaactac cgcttcgatc gctatcatac cgagaccgac
     2041 tcccgccctc agccggtaca aaaggtcact ctgctggaac gggacggacg gaatatcgtg
     2101 cgcatgaaaa aaggcgcggc aattggcgag ataatctcct gggcacagtg tttagcgcgc
     2161 gacctatcga atgaaccggg aaacgtcctc acgccgacca aactggccga aaccgctgcc
     2221 gacctcggca aaaaatacgg cttcaaaact cagattctcc gcaaagcaga aattgaaaaa
     2281 ctgaaattcg gcgccctgct cggcgtggcc cgcggctcgg ctgaaccgcc gaccttcacg
     2341 gttatggagt atcaaccttc taagcgcgcc ttcggcccga ttgtcttggt gggcaagggc
     2401 gtcaccttcg attccggcgg catctccatc aagccggctg acaacatgga cgagatgaaa
     2461 ggcgatatga ccggcggcgc ggtcgtgatt gctacaatta ccgctgccgc gcggttgcag
     2521 ttgccggtgc atttgattgg gctggttccg tccaccgaaa acctcccctc cggcacatcg
     2581 tataaaccgg gtgacgttct gcggtcgtat ttaggaaaaa caattgaaat catcaacacc
     2641 gacgccgaag gacgattgat tttggccgat gctctggcct acgcgcagac cttcaagccg
     2701 aaagccgtta tcgacgttgc caccttgact ggcgcagtga aaatagcttt gggaacggtg
     2761 gccgccggac ttctggccag cgatcagaaa ttagccaacc ggctcaaagc cgctgccgac
     2821 aaatcagccg aaaaggtgtg gcagctgcct ctctgggagg agtatcagga gcggattaag
     2881 tcggacattg ccgacgtgaa aaactccggc ggccgtccgg gttcgaccat caccgccgcc
     2941 tgcttcttac aaaaatttgt cggtgactcc ccctgggcac atttagacat cgccgctatg
     3001 gacatcgagg aaaaaggtca gccctacgtc cccaaaggcg gcaccggctt cggctgcc
//
LOCUS       DBNGZD010000133         6790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_509033, whole genome shotgun sequence.
ACCESSION   DBNGZD010000133 DBNGZD010000000
VERSION     DBNGZD010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_509033"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC16GM_03645"
     CDS             complement(<1..256)
                     /locus_tag="AC16GM_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922802.1"
                     /translation="MRRLVCLGVAALALISCGKSIRLKNYPGPAVPLAFRLDGAIQFP
                     LSVGWSYKASGALTMPVAVNQRYLFFGTLNRKFTVLDRFTG"
     gene            complement(256..3045)
                     /locus_tag="AC16GM_03650"
     CDS             complement(256..3045)
                     /locus_tag="AC16GM_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR2922803.1"
                     /translation="MRRLGATLVTLATVIWGSTPLWAQFYFGQNKVQYTRFDWQVLTT
                     DHFTIYFYPEEQGVAGIAAASAEESYTFLGNKFNHHIEKRTPLIIYSSPHYFEQTNVV
                     PGLLPENVGGFTEFFKGRVVLPYQGSYSEFVKVLRHELVHVFTFDKLTKVLRSHRKTT
                     AAVPPLWFTEGLAEFWSRPEETGATMILKDLVIGGNLVGMDNIASISGSYLMYKEGES
                     FCRFLSQTYGEERILLLLENWWQGRSFEEIVRLTYGKPLGELGREWEYALKKRFFPEI
                     ASHDLPDKKYSQLTQDGFNVKAVPVQLAKNGKTEDWIVFKANKLGYSGLYLMPLAGEK
                     KKLETLVKGERSRTFESLHLLQSGIDGNARGQVVFGSKNNEKDNLYVYDIPRHTITLA
                     AGFDSLVGVLSPVWIPGRQAVVFTGVDKGGFSDLFLYDLESRSLRRLTHDYYEDTDPA
                     VAPDGQEVAFSSDRGSSGKSGTKNIFVLDLATGVITPRTSGAHDDLTPSWSPDGRWIV
                     FTSDRNGPANVFAVNDSNQTVQLTNVLTGVSDPKFLPDGRHLLFTAFKDFSFQIFSTP
                     LDSTLWQKPAPEPQIPVIAWVPDTLSGNLAQGAVKYQKDFSFDIAQSAVAYDNVFGTV
                     GGFQTAFTDILGNQQLYFLLSNNARTRKQFFSSFNAGVTYVNKTHRVNFGAGAFHFYD
                     EYYDDFDGFIAERQVGGLVFASYPVSKFQRFETSFFLRYSNKDFQYFFDRRKALLATN
                     YLSWIRDNSLWDVVGPIDGHRYNLTVGLTSDLTTGRQYNRSVLADGRRYFRLGRHSAL
                     ALRLFGFSSTGLEPQRLYLGGSWSLRGFSRRAFYGRHLILSSTELRFPLIDNLSIRFP
                     MATLGFQAIRGALFFDVGNAWNDQFGRLRGSFGIGARVGLGYLAVLRFDLARTTDFRT
                     IAPNNKFDFFFGWNF"
     gene            3422..3964
                     /locus_tag="AC16GM_03655"
     CDS             3422..3964
                     /locus_tag="AC16GM_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922804.1"
                     /translation="MRCLLLIVLVGLWLASPYGAVPLSAQSAPLAPDTVPPCDHDIYG
                     VCDTVKFFLCPCTDSTKFRIRVWLWNDEPVREILGPSAIFSIGTFFPHYDSVSYRASR
                     PELLPWFRLPLIGYMNFVSPHTFDHTMDADVLTPPTVGRRTSIRRLVFPARPGGGVLV
                     ELKLGERNPYSLSASSRASL"
     gene            complement(4208..4339)
                     /locus_tag="AC16GM_03660"
     CDS             complement(4208..4339)
                     /locus_tag="AC16GM_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922805.1"
                     /translation="MSGAVGAPLWAASGTAPYELAIHKPTIIAKMRRRTFTFPSFFA"
     gene            4667..4996
                     /locus_tag="AC16GM_03665"
     CDS             4667..4996
                     /locus_tag="AC16GM_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922806.1"
                     /translation="MFDIWFSQPGSEGEFWWYSPPGNTILDVDTFLPPNSIRPFVAGA
                     GLLKVPRFGDVDADGLTDLKDIVTLIREVVFATEPSKRGEVTCDGIVDIEDIVAIINW
                     VVFSRGP"
     gene            5036..>6790
                     /locus_tag="AC16GM_03670"
     CDS             5036..>6790
                     /locus_tag="AC16GM_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922807.1"
                     /translation="MERGKTGIVWGLLFTALVLFPAHVNAQCSGGPLQIDSNTTWSGT
                     ITLGRDVVVNPGKTLTIISGTTIRVRTSDSSCSGIDPNKIELTIKGTLVAIGGTGAND
                     TIRFKSDAASPAQGDWYGIVDSGQTRQRYNSLQHARYAVLDRGSAADTLEFSTVTNTA
                     VACSTLNFNLAFRSNNVSGGRHGLVTDVPTSGNLVIDGNSFTNQTDAAVRIARAVSPG
                     TVKITNNRITVADSAVRGIFLERSADTNLIANNVIQGYFTQGHIRIETPGIQLKTTLI
                     NDNRLITGAGTKRSGTGLYVDVSSIAPNIVFKARRNVISGFTVDGVFNGGEPIDLGNV
                     AAGDPGLNTIVTDTTPLFAAVDNRYSEDTLHAEGNWFGMVNPPSSIFSSFVDFDSSVT
                     APLDSVDGKVRVYALTAIAWGPGTANVKGDLTVDSNLTWTLKPNLTLSFKPNADSKAS
                     GLDVNKGEVIVSNGGKLRVEGTSTNRVVFTSPASNDSAWLAVRILPGGRGFADNAKFE
                     RAYSAWQWGGGGAVDTVRSSIFDHDYLHGILCKDTNAVILSNTFTNILHVGPANDTVG
                     YGIRVQSASPQVKTNRLTA"
BASE COUNT         1467 a         1884 c         1999 g         1440 t
ORIGIN      
        1 tccccgtaaa gcggtcgagc accgtgaact tgcggttcaa ggttccgaag aagaggtagc
       61 gttgattgac cgccacgggc atcgtcagag cgccgctggc tttgtaactc cagccgactg
      121 aaagggggaa ctggattgca ccatccagtc gaaatgcaag cggtacggct ggccctggat
      181 agttttttag ccggatcgac ttgccgcagg agataagagc cagggctgcc acccccaggc
      241 agaccagacg ccgcattaaa aattccagcc gaagaagaag tcgaacttat tgttgggggc
      301 aatggtacgg aagtcggtgg tacgggccaa atcgaaccgg agcactgcca ggtagcccaa
      361 ccccacccgg gcgccgatgc cgaaactgcc ccgcagccgg ccgaactggt cgttccaagc
      421 gttgcccacg tcgaaaaaga gcgccccgcg gatcgcctga aaccccaggg tggccatcgg
      481 gaagcggatg gagagattgt cgatgagcgg gaagcggagt tcggtggaac tcagaatcaa
      541 atgcctcccg tagaaggccc gccgggagaa accgcgcaac gaccaggagc cgcccaggta
      601 gagcctttgg ggctccaggc cggtggagga aaaaccaaag agccgcagcg ccagggccga
      661 atgccggccc aagcggaagt agcgccggcc gtcggccagg acgctccggt tgtactgccg
      721 gccggtggtg agatcggagg tcagacccac cgtaaggttg tagcggtgcc cgtctatcgg
      781 ccccaccacg tcccagagac tgttatcccg gatccaggag aggtagtttg tggccaaaag
      841 cgccttccgc cggtcgaaga aatactggaa gtccttgttg gagtaacgga gaaagaaact
      901 cgtttcgaac cgctggaact tggaaaccgg ataggaagcg aagacgagcc cgcccacctg
      961 gcgctcggcg atgaagccgt cgaaatcgtc gtagtactca tcatagaaat ggaaggcgcc
     1021 ggccccgaag ttcacccggt gggttttatt cacgtaggtg acgccggcgt tgaaactgga
     1081 gaagaactgc ttgcgggtgc gggcgttgtt ggagagcaaa aagtagagct gctggttgcc
     1141 caggatgtcg gtgaaggcgg tctggaaccc gccgaccgta ccgaagacgt tgtcgtaggc
     1201 caccgccgac tgggcgatat caaaggaaaa atctttctgg tatttgaccg cgccctgcgc
     1261 caggttgccc gagagggtgt cgggcaccca ggcgatcacg ggtatctgcg gttccggagc
     1321 cggcttttgc cagagggtgc tgtccagcgg ggtcgagaag atctggaagg aaaaatcttt
     1381 gaaggcggtg aaaagcaggt gccggccgtc cggcaggaac ttcgggtcgg agacgccggt
     1441 gaggacgttg gtgagctgga ccgtttgatt cgaatcgttc acggcgaaaa cgttggccgg
     1501 gccgttccgg tcggaggtga aaacgatcca gcgcccgtcg ggcgaccagg agggtgtcag
     1561 gtcgtcgtgg gcgccggagg tcctgggggt aatgacgccg gtagccagat cgagaacaaa
     1621 aatatttttt gtgcccgatt tccccgacga accccggtcg gaactgaagg ccacctcctg
     1681 tccgtcgggc gcaacggcgg gatcggtatc ctcgtagtaa tcgtgggtca ggcgcctgag
     1741 actccgactt tccaggtcgt acaagaaaag gtccgaaaaa ccgcccttgt cgacgccggt
     1801 gaaaacaact gcctgccggc ccgggatcca gaccggtgag agaacgccca cgagcgagtc
     1861 aaaaccggcg gccagagtga tcgtgtgcct ggggatgtcg taaacgtaga ggttgtcttt
     1921 ctcgttgttt ttggaaccga acaccacttg cccgcgggca ttaccgtcaa tgccgctctg
     1981 cagcagatgg agcgattcaa acgtccggga gcgctcgcct ttgactaagg tttccagctt
     2041 ctttttctcc ccggccagag gcatcaagta gaggcccgaa taccctaatt tattcgcttt
     2101 gaagacgatc caatcttcgg ttttgccgtt tttggcgagt tgcaccggga ccgctttaac
     2161 gttgaaaccg tcctgggtca actgggaata ctttttgtcc ggcaggtcgt ggctggctat
     2221 ctccggaaag aagcgttttt tgagcgcata ctcccactcg cggcccagtt cccccagcgg
     2281 cttgccgtag gtgagccgga cgatttcctc gaaactgcgt ccctgccacc agttctccag
     2341 caagagcagg attcgttctt cgccgtaggt ttgggagagg aaccggcaga aggactcccc
     2401 ttccttgtac atcaggtaac tgccggaaat cgaagcgatg ttgtccatcc cgaccagatt
     2461 gccgccgatg acgagatctt tcaaaatcat cgtggcaccg gtctcctcgg gccgggacca
     2521 gaactccgcc aagccctctg tgaaccataa gggcggtacg gcggcggtag tcttgcggtg
     2581 ggagcgcagg accttggtca gtttatcaaa agtaaagacg tgcactaatt cgtgccgcaa
     2641 aactttgaca aactcgctgt aagagccctg gtaggggagc accacgcggc ctttgaagaa
     2701 ctcggtgaag ccgcccacgt tttccggcag aagcccgggg acgacgttgg tctgctcgaa
     2761 atagtggggc gaggaataga tgatgagcgg ggtgcgtttc tcaatgtggt ggttgaactt
     2821 gttccccaga aaagtgtagc tctcctccgc cgaagccgcc gcaatgccgg ccacgccttg
     2881 ttcttccggg tagaagtaga tggtgaaatg gtcggtggtg aggacctgcc agtcgaacct
     2941 ggtgtactgg actttgttct ggccgaaata gaattgggcc caaagcgggg tgctccccca
     3001 gatgactgtg gcgagggtca cgagtgttgc gccgaggcgt ctcattagtc tactcccgtg
     3061 cggggaattc cctctcccaa ttcccattct ctctacacct gctacgttca gctttcggca
     3121 gtaatttgca taaaggcgct ggcccggcct ccgctggccg gccgttcatt ttttgaccac
     3181 cggcatctga ttataagata cgatgttcct tcgctccgtg gcaagttcca gataccctta
     3241 gccatcgcgc tcttcatata ccgcaagccc tttgccgtta ggggaagaat tgaaggcggt
     3301 tgccggctgc ggggactggt acggcatcgt aatcgagtcg ggcgggaaga cgagagatgg
     3361 gaacataaca cctaaaccag cggttacgta taattgaacg ccaacatcgg ggtgcgcagt
     3421 aatgcgttgt ttattgctca tagttctggt tggcttgtgg ctggccagcc cctacggggc
     3481 agtgccgctt tctgcccaga gcgcgccttt agcgccggac accgttcccc cctgcgacca
     3541 cgatatctac ggcgtctgcg acacggtcaa attcttcctc tgcccctgca ccgactcgac
     3601 caaattccgc atccgggtct ggctctggaa cgacgaaccg gttagagaga tcctcgggcc
     3661 gagcgccatc ttttccatcg gtaccttctt tcctcactac gattcggtga gttaccgggc
     3721 gagccgcccg gaacttttgc cctggttcag acttccgctg attggttata tgaactttgt
     3781 ctcgccccat acgttcgacc acactatgga cgcggacgtt ttaacccccc ctaccgtcgg
     3841 gagaaggacc tctatacgac gtctggtttt cccagcccgg cccggagggg gagttcttgt
     3901 ggaactcaag ctcggggaac gtaatcctta cagtttatcc gcctcctccc gtgcctcctt
     3961 ataattttaa cccgttcatg tcagccactg gcgtgcttag agtaacccgc tttggggatg
     4021 tgaatgccga cggggtagcg gatatagtgg acattatctc tctcatccac gaggtggtct
     4081 ttgcggcaga gccttcaact cgaggggagg tgacctgtga tgggctgata gacatagtag
     4141 acatcgtgac cctcatcaac tgggtcgttt tcggaaacaa ttcagtcgca ggacaggtta
     4201 cgtataatta agcaaagaaa ctggggaaag tgaaagtgcg gcgtctcatt ttggcaataa
     4261 tggtcggctt gtggatggcc agctcgtacg gggcagtgcc gcttgctgcc cagagcggtg
     4321 caccaacagc gccggacacc gttcccccct gcctgtacga tcagtttggg gtttgcgata
     4381 cactcaaatt catcgtctgc ccctgcaccg actccaccaa gttccgcctc cgagtctggc
     4441 tctggaatga tgagcggatt aactcggtta atggtcccgt ggcgatcttt tccaccggga
     4501 ccttctttcc gcattacgat tcagtcagtt accgggatag tcgcccagaa ctcctgcctt
     4561 ggagcagatt tccctcacag cgcatggact tcgtctcgcc cgatactttc gaccacacga
     4621 tggacgctga cgttataacc cccccctacc gtcgggggaa gggccattgt ttgacatctg
     4681 gttttcccaa cccggctcgg aaggggaatt ctggtggtac tcacccccgg gcaacacgat
     4741 tctagacgtt gatacgttcc taccccccaa ttccattcgc cctttcgtgg ctggcgctgg
     4801 tctgcttaaa gtacctcggt ttggagatgt ggatgccgac ggactgaccg accttaagga
     4861 tatagtaaca ttgatccgtg aggtcgtatt cgcaactgaa ccttcaaaaa gaggggaggt
     4921 gacctgtgat ggaatagtcg acatagagga catcgttgcg atcatcaact gggttgtgtt
     4981 cagcagaggt ccgtagtttt agaaccaatg ttgtggcagc aaaggaggtg cagcgatgga
     5041 acgcggtaag acagggatag tctggggatt actctttacg gcactggtgc ttttcccggc
     5101 gcatgtgaat gctcaatgca gcggtggtcc gctccaaatt gactccaata ccacctggag
     5161 tggcaccatc accctggggc gggacgtggt tgtcaacccc ggaaaaacgt taaccataat
     5221 ctctggcaca accatacggg tgcgcacgtc ggattccagc tgttccggca tcgacccgaa
     5281 caagatcgaa ttaaccatca aaggcacgct ggtggctatt ggcggaacag gcgccaacga
     5341 caccattcgt tttaagtccg acgcggcctc tccggctcaa ggcgattggt acggcattgt
     5401 ggattccggc cagaccaggc aaaggtacaa tagcctccag cacgcccgct atgctgtcct
     5461 ggatcgtggg agcgccgctg atactctgga attcagcaca gtcacaaaca cagcggtggc
     5521 ctgctcaact ttgaatttca acctcgcttt tcgcagtaat aacgtctccg gagggcgcca
     5581 cggactcgta acggacgtac cgacgtctgg caacctcgtc attgacggca acagtttcac
     5641 aaatcaaacg gatgcggccg ttaggatagc cagagccgtc agtccgggga cggtcaagat
     5701 caccaataac cggatcaccg ttgcagatag tgcggtgcga ggaatctttt tagagcggag
     5761 cgccgacacg aacctgatcg caaataacgt catccagggc tactttaccc agggtcatat
     5821 tagaattgaa acaccaggca tccaacttaa gaccacgctc atcaacgaca accggctcat
     5881 caccggcgca ggcacgaaac ggtcgggcac gggattatat gtcgatgttt cctcgattgc
     5941 ccccaacatc gtcttcaagg cgcggcgcaa cgtaatcagc ggttttactg ttgatggtgt
     6001 gttcaacggg ggcgagccga ttgatttagg aaacgttgcg gcaggcgacc cggggctgaa
     6061 cacgattgtc accgatacta ctcccttgtt cgcagcagtt gacaacaggt attcggaaga
     6121 caccttgcat gcggaaggga actggttcgg gatggtgaac ccaccctcgt cgatcttttc
     6181 cagtttcgtt gacttcgatt cgtccgtcac tgctccctta gattcggtgg acgggaaggt
     6241 tcgtgtgtac gcgttgaccg ccattgcctg ggggccgggc acggccaacg tcaagggcga
     6301 cctgacggtg gattccaatc tcacgtggac gctcaagccc aatctgacac tctcgttcaa
     6361 accgaacgcc gattcgaaag ccagcggtct tgatgtgaac aagggcgagg tgattgtgtc
     6421 taacggcggc aagttacgcg tcgaaggtac gagcaccaat cgcgtggtat tcacctcacc
     6481 ggccagcaac gactccgcct ggttggcagt gcgcattctc cccggtgggc gggggtttgc
     6541 ggacaatgcc aagttcgagc gggcctactc ggcctggcag tggggcggcg ggggcgcggt
     6601 ggatacggtg cggagcagca tcttcgacca cgattacctg catggaattc tgtgcaagga
     6661 taccaacgcg gtgattctct ccaacacctt cacgaacatc ctgcacgtgg ggcctgccaa
     6721 tgacacggtg ggctacggca tacgggtgca gagcgcctcg ccgcaggtga aaacgaaccg
     6781 tctgaccgcc
//
LOCUS       DBNGZD010000134         3488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_92450, whole genome shotgun sequence.
ACCESSION   DBNGZD010000134 DBNGZD010000000
VERSION     DBNGZD010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_92450"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3139)
                     /locus_tag="AC16GM_03675"
     CDS             complement(<1..3139)
                     /locus_tag="AC16GM_03675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR2922808.1"
                     /translation="MKHADFVHLHNHTQYSLLDGACRIEAMLDMAAHFKMPALAITDH
                     GNLFGTIDFYKQAKAKGIKPIIGCEAYVAPESRFKKEPLRGFPDAGYHLILLVKNQTG
                     YKNLVKLVTAGYLEGFYHRPRMDKELLKAHAEGLVALSSCLNGEVPAQLRNNDVKKAK
                     EMAEEYLKIFGEGNFFIELQDHGLEEEAKIRRALAKVAGDLDISLVGTNDCHYLKREH
                     AQAHDALLCIQTGKLLTDTDRLKYETDQIYFKSPAEMKALFADYPDAVEHTLRIAELC
                     NLELELGKIHLPHFPLPHGFESNDQYLEYLARKGLTHRYSHVTPALEERLDYELKIIR
                     QTGYAGYFLIVKDFIDYARHQNIPVGPGRGSAASSIVSYALGITSIDPIKYGLVFERF
                     LNPERISMPDIDIDFADRDREKIIEYVTRKYGQANVSQIITFGTLAARGVIRDVGRVM
                     ALPYSEADRIAKLIPFEIDMTIDRALELEPDLKRLADSDPKVQKLLDFSRTLEGLTRH
                     ASTHAAGVVIAPTELTNYVPLYKTNKDEVITQFDMKGIEEIGLLKIDFLGLRTLTVIQ
                     ETVRLVLEHHGLALDIENIPLDDQKVYDLFGRGDTVGLFQFESSGMRDYLRKLKPTGL
                     EDLAAMNALYRPGPLDSFMVDEFIARKKGEKQIVYEHPLLEPILRETYGVIVYQEQVL
                     RIASELAGYSPGKADILRKAMGKKIAELMQEQRAEFIAGARARGIRKETAEKTFELIA
                     TFGRYGFNKAHSVGYALLAYQTGYLKIHYPVEFTAASLTSEMGNTDRIVILMDECRRM
                     GIEVLPPDVNESNATFSVVGNQIRFGLAAVKNVGENAVAAIVEARGKFGRFQTLFQFC
                     ERVDLAAINKRALESLIMAGACDSLKGHRAQLLLAIPDALGYGQLANEERKRGQTSLF
                     AGGAKHTPVPEPRLPDAPEWSLAEILSKEKEMLGFYVSGHPLTKHQDHLRLYATHTSE
                     ALATVRDGQEVTVGGIVVAVKTNIDKKGKQMAFATLEDFAGTIETVIFADLYEKNKGL
                     VRADQMILLRGRASTK"
     gene            complement(3200..>3488)
                     /locus_tag="AC16GM_03680"
     CDS             complement(3200..>3488)
                     /locus_tag="AC16GM_03680"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR2922809.1"
                     /translation="GVLGAYAVKFPRAGIWTLLFLGFFVRVVKIPAMMVLGFWFIIQL
                     ANALPALGTEVVGGVAWFAHIGGFLAGIVLFGLFKLFGSAAKPAESQSETE"
BASE COUNT          700 a          976 c         1035 g          777 t
ORIGIN      
        1 ctttggtgga cgctcgacca cgcaaaagga tcatctggtc ggcccgcacc aggcctttgt
       61 tcttctcgta caggtcggca aagatgaccg tctcgatggt gccggcgaag tcttccagcg
      121 tggcaaaggc catctgtttg cctttcttgt cgatattggt tttcaccgcc accacgatgc
      181 cgccgacagt aacttcttgc ccgtcgcgga ccgtcgcgag cgcttcgctg gtgtgggtcg
      241 cgtacaagcg gaggtggtcc tgatgcttgg ttaatggatg gccggagacg tagaagccca
      301 gcatttcttt ttctttggaa agaatttcgg caagcgacca ctccggcgca tcgggcagtc
      361 gcggctccgg taccggagta tgtttggcgc cgcccgcaaa aagggaggtc tggccgcgtt
      421 tgcgctcttc attcgccagt tgaccgtaac ccaacgcatc cggaatggcg agcaaaagtt
      481 gcgcccggtg acctttcagc gagtcgcagg caccggccat aatcaagctc tccagggcac
      541 gtttattgat agccgccaga tcgacccgct cgcagaactg aaaaagagtc tggaaacggc
      601 caaacttccc gcgggcttcc acgatggcgg ccacggcgtt ctcaccgaca ttcttcaccg
      661 cggccagccc gaagcggatc tggttcccca ccacgctgaa agtggcgttc gattcgttga
      721 cgtcgggcgg cagaacctcg attcccatcc ggcggcactc gtccatcagg atcacaatcc
      781 ggtcagtgtt ccccatttcg gaggtgaggg aggcggccgt gaactccacc ggatagtgaa
      841 ttttgaggta gccggtttgg taagccaaaa gtgcgtagcc gaccgagtgg gccttgttaa
      901 acccgtagcg tccgaaggtg gcgatcagtt cgaaggtctt ttcggcggtt tctttcctaa
      961 tcccgcgcgc gcgggcgccg gcaatgaact cagcacgctg ctcctgcatt agttcggcga
     1021 tttttttgcc cattgccttg cgcaggatgt cggctttgcc cggagaatag ccggccaact
     1081 cggaggcaat ccgtagcacc tgttcctggt agacgatgac gccgtaggtt tctcttaaga
     1141 tcggctccaa gagggggtgc tcgtagacga tttgcttctc gcctttcttg cgggcgatga
     1201 attcgtccac cataaaagag tccaaagggc ccggacggta gagggcgttc atggcggcca
     1261 agtcttccag gccggtgggc ttcagcttcc gcaggtagtc acgcatgccg gacgattcga
     1321 actggaaaag ccccaccgtg tcgccccggc cgaagaggtc gtagactttc tggtcgtcca
     1381 gaggaatatt ttcgatgtcc agcgccaagc cgtggtgctc caagaccagc cgcaccgttt
     1441 cctgaatgac ggtcaaagtc cgcaggccga gaaaatcgat tttcaaaagg ccgatctcct
     1501 cgatgccttt catgtcgaac tgggtgatca cctcgtcctt attggttttg tagagcggga
     1561 cgtagttggt cagctccgtc ggcgcgatca ctacgccggc ggcgtgggtg gaggcgtggc
     1621 gggtcaggcc ttccaaagtc cgggagaagt cgagcaattt ttgcacttta gggtcggagt
     1681 cggccaggcg cttcaaatcc ggctccagtt ccagcgcccg gtcgatggtc atgtcaattt
     1741 caaaagggat cagtttggca atccgatccg cttccgaata cggcagggcc atcacccggc
     1801 ccacgtcgcg gatgacgccg cgggcggcca gcgtgccgaa ggtgatgatc tggctgacgt
     1861 tggcctggcc gtacttgcgt gttacatact cgataatttt ctctcggtcg cggtcggcaa
     1921 agtcgatgtc gatgtcgggc atcgagatcc gctccgggtt caaaaagcgc tcgaagacca
     1981 gtccgtactt aatcggatcg atactggtaa tccccagcgc ataggaaact atactcgaag
     2041 ccgccgaccc gcggcccggt cccaccggga tgttctggtg gcgggcgtaa tcgatgaaat
     2101 ctttgactat cagaaaatag ccggcatagc cggtctggcg gatgattttc aattcatagt
     2161 ccaaccgctc ttccagcgcc ggggtgacgt gcgaataacg gtgcgtcagc cctttgcgag
     2221 ccaaatactc aaggtactgg tcgttcgact caaatccgtg cgggaggggg aaatggggca
     2281 ggtggatctt gcccagttcc aactccaggt tgcacagttc agcgatgcgc agggtgtgct
     2341 ccaccgcgtc gggatagtcg gcaaagagcg ctttcatctc ggcgggcgac ttgaagtaaa
     2401 tctgatcggt ttcgtacttc agccggtcgg tgtcggtcaa aagtttaccg gtctggatgc
     2461 agaggagggc gtcgtgggct tgggcgtgct cacgtttgag gtagtggcag tcgttggtcc
     2521 caacgagaga gatatcgagg tcgccagcga ccttcgccag ggcgcggcgg attttggctt
     2581 cttcctccag cccgtggtcc tgcagttcaa taaagaaatt cccctcaccg aagattttca
     2641 gatattcttc cgccatttct ttcgcttttt tcacgtcatt attgcggagc tgagctggca
     2701 cttcgccgtt gaggcaggaa gaaagcgcca ccaacccctc ggcgtgtgct ttgagcagtt
     2761 ccttatccat ccgcgggcgg tggtagaagc cctcgaggta gcccgcggtg accagtttaa
     2821 ccaggttctt gtagccggtc tggtttttga ccagaaggat caagtggtac cccgcatcgg
     2881 gaaagccgcg caacggctct tttttgaagc ggctttccgg ggcgacgtag gcctcgcagc
     2941 cgatgatcgg cttaatccct ttcgctttcg cctgcttgta gaagtcgatt gtcccgaaga
     3001 ggttcccgtg gtcggtgatg gccagggcag gcatcttgaa gtgcgcggcc atatcgagca
     3061 tcgcctcgat gcggcaggcg ccgtccagaa gcgagtactg ggtgtggtta tgcaggtgga
     3121 cgaaatcggc gtgcttcata agtcaacgga ttcggagcgg atttaaacgg attcatgacg
     3181 tgatcacttt cagattccct cattctgttt cagattgaga ttcagctggc ttggccgccg
     3241 aaccaaaaag tttgaacaac ccaaagagca ctatccctgc cagaaagccg ccgatgtggg
     3301 cgaaccaggc aacgccaccc accacttcgg tgcccagggc cggcagggca ttggccagct
     3361 ggatgatgaa ccagaagccc aggaccatca tagcgggtat tttcactacc cgcacgaaga
     3421 aacccaaaaa gagaagcgtc cagattcccg cgcgggggaa tttaacggcg taggctccca
     3481 gcacgccc
//
LOCUS       DBNGZD010000135         8500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_370439, whole genome shotgun sequence.
ACCESSION   DBNGZD010000135 DBNGZD010000000
VERSION     DBNGZD010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_370439"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..806
                     /locus_tag="AC16GM_03685"
     CDS             147..806
                     /locus_tag="AC16GM_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR2922810.1"
                     /translation="MEKRRGPEKVRRLFLAGVLVIVPLIATFIVLRFLFRSLDGLLGP
                     PLQHLIGHEIPGIGIVATLVLIFLAGFLTSNLVGSRLYGIAELFFIRAPIVRTVYSAA
                     KQFVEAIAVPSKRQFERVVLVQYPRAGSYALGFVSKSIALTGFPGRDKLVAVFVPSTP
                     TPFTGFAVLLPPDEIIPLDLSLEEGVKFIVSGGIASPETFNVKLPPERNALPHAISRP
                     A"
     gene            784..1689
                     /locus_tag="AC16GM_03690"
     CDS             784..1689
                     /locus_tag="AC16GM_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR2922811.1"
                     /translation="MQLADLLDPELILLDLDCATKREAVERLVEIAVKKYPELSAEDL
                     MNVIFEREEVESTSLGRGFAFPHARIDQVTGLIFAVAVTRRDLPEKTIDAKPLRVICL
                     FLTPRHMARLYLQTMSALVSFARTEGLVEHLLQAKTAEELILFFRKANIEVRRDLVAR
                     DIMRRQVVKATLQMSLKEVANLLFKHRISGVPVVNAEEEVVGEVTEKELIKAAIPNYS
                     RLVDNARAHLEVDPFETLIQKEDKITARDLMASKFVTRSEETPVVELAALMLLNNVRR
                     VEIVRDHKLVGIVVRSDIVGKIIRG"
     gene            1689..2897
                     /locus_tag="AC16GM_03695"
     CDS             1689..2897
                     /locus_tag="AC16GM_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922812.1"
                     /translation="MRFQETAESILAGAAKGKALSALWTFPSILFAAFCIAWAAEAAQ
                     VLVSRGLALAVLAWLQTAPEFAVDGVIAWRAGKVPTPENIGLMTANFTGALRLLVGFG
                     WPGVYFMCWLGHRRKGNSGLPVIRMEGEDSIPIVGMIVPLLYAFFIAIKGSLSLWDAA
                     ILIFIYSIYLWILYRVPPRQEEDLEEMERLPKWILSHTKPRRNALILLFFGAGAILII
                     TMAHPFVESMLGVAVWVGISSYFAVQWIAPFLSESPEFLSAFYWARRSAKAPMALLNT
                     VSSSIAELTLLIALIPIVYSLSGGHFSAVPFDGPHRMEILLTAVQALLGFLLLANFRF
                     HWYEAAGIFALWFVQFIIPHLREEVIFAYGFWILVEFTLIMVGQQQLPAFAAFGRCLR
                     QHGILVKKSA"
     gene            3004..3477
                     /locus_tag="AC16GM_03700"
     CDS             3004..3477
                     /locus_tag="AC16GM_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922813.1"
                     /translation="MHLVALVGAAMLVLGVFLPWMQVGIFQERGIDALDGGLVLAAGL
                     LGLIVAGHNLARGRNEYRWVYLPDGVLALIISLVALARIGQRAAKITGALEEILRIFG
                     GTEKIKTSELVGSGLVVVIIGSMLLLAAGLGQVVGSKFAAPRGDTEGTSSAKENW"
     gene            3477..4097
                     /locus_tag="AC16GM_03705"
     CDS             3477..4097
                     /locus_tag="AC16GM_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR2922814.1"
                     /translation="MGLLPAKEKFFDMLDRAAKNVTQGVEEFQAMLADYAHREEHVKK
                     LDAMEHEGDIITHEIMDKLNRSFVTPIDREDIHALASGLDDVIDVVEEAAHRMYLYRI
                     EKPNPRLLEQAEILLKAVQEVCRAVTSLRDLKRPRRILDYAIEINRLENEGDVALNRN
                     VAALFEGQVAPLEVIKWKEIYEHVEAALDKCEDIAVIIESVVVKNA"
     gene            4217..5218
                     /locus_tag="AC16GM_03710"
     CDS             4217..5218
                     /locus_tag="AC16GM_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR2922815.1"
                     /translation="MIELSAGLVVVLVLVLAAEFVNGWTDAPNAIATVVSTRALSPGK
                     AVVLAAVLNAVGALSGTAVAATIGKGIVRPEVINLTTVGAAMVAIVFWSTLAWKYGLP
                     TSESHALVAALAGAGLATAGPSVLLWVGWKKVLIGLVFSTFLGFGVGWAIVKVILRFF
                     AQMTPTRARKVFGRMQLVSAAFMAFSHGSNDGQKFIGTFSLALVLGGVLPEFRVPWEV
                     ILICSLIMGISTSVGGWRIIKTMGMQMVKLETYQGFSAEAGAASSIELASRLGIPVST
                     THTIGTALMGVGASRRLSAVRWGVAGQVVTAWILTFPVCGFIGWLVATVFGMFFHSR"
     gene            5433..6284
                     /gene="htpX"
                     /locus_tag="AC16GM_03715"
     CDS             5433..6284
                     /gene="htpX"
                     /locus_tag="AC16GM_03715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIR2922816.1"
                     /translation="MKVFKTVLLLTVLTLLFVFIGNLIGGQQGMLIALIFAFAMNFFA
                     YWFSDKMVLAMYRAKPVTESDNPRLYRIVRSIATKASIPMPKVYTIPVDTPNAFATGR
                     NPQHAAVAATEGILRLLDDNELEGVIAHEVSHVRNRDILIGTIAATIAGAVMFLSHLA
                     QWGAIFGGGGGRDSENRGNAFALLAVAILAPFAAMLVQLAISRSREYAADETGARISR
                     QPLALASALKKLQICNARLPMQANPATAHMFIVSPFSARGLANLFSTHPPVEELVARL
                     ETLARQF"
     gene            6766..8103
                     /locus_tag="AC16GM_03720"
     CDS             6766..8103
                     /locus_tag="AC16GM_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR2922817.1"
                     /translation="MPEYLVQLILIFALILANSFFAGSEIALVSARRSKIRQLAEAGD
                     TNARIAEALQNQPERLLATVQVGMTVVSTLAGVIGGATIVAVLQKELAQVPVTFIQKG
                     SQTIAIAIVVVSISYLTIVVGELVPKYIAYVYSETLATRLARPVHWISRIFSWAIKLL
                     SVSSRLVVRAIGIKSLPESGAVSEEEIKLLLREGQKKGIFEKTEQELIHSVFEFTDTT
                     VRMAMTPRTDIVAIDLAWDQKRLLRAVTEEGYSRYPVYQGSLDNVVGIIHTKDIINLL
                     VDSELIILQDILRKPYFVPDSKKIGELLRDFQRLQVHLAIVLDEFGGTAGLITLEDIL
                     EEIVGEIQDEYDIEAERYVRLPDGSVWVAARLSLSELREILPGELPPGADADTIGGLV
                     TSALGRIPSLSEAVNAGDYRFTIMEKAGHRITRVRVEKIQNTAYRTQNTGENH"
     gene            8159..>8500
                     /locus_tag="AC16GM_03725"
     CDS             8159..>8500
                     /locus_tag="AC16GM_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR2922818.1"
                     /translation="MVSKILEAVGGWIVALISSSGYAGVVIAMTIESACIPLPSEIIM
                     PFAGYLVFTGRFTMLGASVAGAFGCLVGSILAYGIGYYGGRPLAVKYGRYVLLSQHDL
                     DKADRWFARWGN"
BASE COUNT         1635 a         2354 c         2635 g         1876 t
ORIGIN      
        1 atctgaaccc gggcgaatct aaagattcac ccctgcattc ttttactgcg cactgcgcga
       61 gtaaactcgc gcctcctgag gttggatgtg cttgacggtc gggaagcagg cggttaagtt
      121 gccttgctcg aaagcaaagc atggcgatgg aaaagcgcag aggtccggaa aaagtccggc
      181 ggctgttttt ggccggggtc ctggtcattg tcccgctcat cgccaccttc atcgtcctca
      241 gattcctctt tcgctcgctg gacggtctgc tcggcccgcc cttgcagcac ttgatcggcc
      301 acgagattcc cggcatcggg attgtcgcca cgctggtttt gatttttctg gccggcttcc
      361 ttacctccaa tctggtcggc tcgcggctgt acggcatcgc cgaacttttc ttcatccggg
      421 cgccgatcgt gcgcacggtc tacagcgcgg ccaagcagtt cgtggaggcg attgcggtgc
      481 cctccaagcg gcaattcgaa agagtagtgt tagtgcagta cccgcgcgcc ggctcgtacg
      541 ctttgggatt cgtctccaaa tcgatcgcac tcaccggctt ccccggccgg gataaactgg
      601 tcgcagtatt tgtcccctcc acgccgaccc ctttcaccgg ttttgccgtt ctcttgccgc
      661 cggatgaaat catcccgctc gatctttcgc ttgaggaggg ggtgaagttc atcgtctccg
      721 gcgggatcgc ttcaccggaa acgttcaacg tgaaattgcc tccggaacga aacgcccttc
      781 cccatgcaat tagccgacct gcttgatccc gaactcatcc ttcttgacct cgactgtgcc
      841 accaaacgcg aggcggtgga gcgattggtc gagatagcgg tcaaaaaata tccggaactc
      901 tcggctgaag atctgatgaa cgtaatcttt gagcgggaag aggtggaatc gaccagtttg
      961 ggccggggct tcgccttccc ccacgcccgg atcgatcagg tgacgggatt aatcttcgcc
     1021 gtcgccgtca cccgaagaga tttaccggag aagaccatcg atgccaaacc cctgcgggta
     1081 atctgccttt tcctcacccc tcggcacatg gcgcggctct atttgcaaac gatgtcggcc
     1141 ctggtatcct tcgcccgcac cgaagggttg gtggagcacc tgctgcaggc caaaaccgcg
     1201 gaagagctca ttcttttttt ccggaaagcc aatatcgagg tgcggcgcga cctggtggca
     1261 cgcgacatca tgcggcggca ggtggtcaag gcgacgctcc agatgagcct gaaagaagtg
     1321 gccaacctcc tgttcaaaca ccgcatctcc ggcgtgccgg tggtcaatgc ggaggaagaa
     1381 gtggtgggag aggtcaccga gaaagagtta atcaaggccg ccattccgaa ctactcccgc
     1441 ttggtggaca atgcccgggc gcacctggag gttgatccgt tcgagacctt gattcagaaa
     1501 gaagacaaga ttaccgccag agacctgatg gcgtccaagt tcgtaacccg cagcgaagag
     1561 acgccggtag tggagttagc agcgctcatg cttttgaaca acgtccggcg cgtggaaatc
     1621 gtccgggacc acaaattggt cggaattgtg gtccgttccg acatcgtcgg caaaatcatc
     1681 cggggctgat gcgcttccaa gagacggcgg aatcgattct ggccggagcg gccaaaggca
     1741 aggccctctc ggctctctgg actttccctt ccattctctt tgcggcgttc tgcattgcct
     1801 gggcggcgga agcggcgcag gttctggttt cccgcggact ggccctggcg gttctggcct
     1861 ggctgcagac cgcgccagag tttgcagtgg acggggtgat tgcctggcgg gcgggaaaag
     1921 tgccgacgcc ggagaatatc gggctcatga ccgccaactt caccggcgcc ttgcggcttt
     1981 tggtcggctt cggctggccc ggagtctatt tcatgtgctg gctgggacac cggcggaagg
     2041 ggaattcggg tttgccggtc attcggatgg aaggagagga ttcgattccc atcgtcggga
     2101 tgatcgtgcc gctcctctac gcctttttta ttgcaataaa aggctcgctt tcgctttggg
     2161 atgcggctat tctcattttc atctactcga tttacctctg gattctgtac cgcgttcctc
     2221 cccggcagga agaagatttg gaggaaatgg agcggctccc caagtggatt ctctctcaca
     2281 ccaagcccag gcggaacgca ctcattctcc ttttcttcgg cgccggcgcc attctgatca
     2341 ttaccatggc ccacccgttc gtggagagca tgttgggcgt agcggtctgg gtagggattt
     2401 cgtcctactt tgcggtgcag tggattgcgc cctttctctc ggagtcgccg gaattcctct
     2461 ccgctttcta ctgggcccgc cgcagcgcca aggcgccgat ggctctgctc aacaccgtct
     2521 cgtcttccat cgccgaattg acgcttttga ttgcgctcat tccgattgtc tattcccttt
     2581 cgggcgggca tttttcggcg gtgccgtttg acgggccgca ccggatggag attcttttga
     2641 ccgcggtgca ggcgcttctg ggatttctct tgctggcgaa tttccgcttc cactggtacg
     2701 aggcggcggg gattttcgcc ctctggttcg tgcaattcat cattccccac ctgcgggagg
     2761 aagtgatttt tgcctacggc ttctggattc tggtggagtt cactctgatt atggtgggcc
     2821 agcaacagtt gccggcgttt gcggcctttg gacgctgttt gaggcagcac gggatactgg
     2881 tgaaaaagag cgcgtaatgc gattaatatt cataaaggcg ctgaaggcca ctaaccatgt
     2941 ctttcgtaac ggacttgagt cttggcgggc cgagggcggc ccgtccacgc ggggaaaagt
     3001 ttcatgcacc tcgtagcgct ggtaggtgcg gcgatgctcg tcctgggcgt ctttttgccc
     3061 tggatgcagg tgggtatttt ccaggagcgg gggattgatg ccctggacgg cggactggtt
     3121 ttggccgccg gcctcttggg gttaattgtc gccgggcaca atctggcccg ggggcggaac
     3181 gagtaccgct gggtctacct gccggacgga gttctggcct taattatcag tctcgtcgcc
     3241 ctggcacgga tcggccagcg ggcggccaaa atcaccggag cgctggagga gatcctccgg
     3301 attttcggtg ggacggaaaa aatcaaaacg agcgaactgg tcggttcggg tttggtggtc
     3361 gtcatcatcg gcagtatgct cttgttggcg gcggggctcg ggcaggtggt cgggagcaag
     3421 ttcgcagcgc cgcggggtga cactgaggga acgagttcag caaaggagaa ctggtaatgg
     3481 gacttttacc cgcaaaagag aagtttttcg acatgctcga ccgcgccgcc aagaacgtca
     3541 cccagggggt ggaagagttc caggcgatgc tggccgacta cgcgcatcgc gaggagcacg
     3601 tcaagaagct ggacgccatg gagcacgagg gggacatcat cacccacgag atcatggata
     3661 aactgaaccg ctccttcgtc acgcccatcg accgcgaaga catccacgcg ctcgcctccg
     3721 gcctggacga cgtgatcgac gtggtggagg aggcggcgca ccggatgtac ctgtaccgga
     3781 tcgaaaagcc gaacccgcgc cttctggaac aagccgaaat cctgctcaag gcggtgcagg
     3841 aggtctgccg ggcggtgacc agcctccgtg atctgaaacg accgcggcgg attctggact
     3901 acgccattga aatcaaccgg ctggaaaacg aaggggacgt ggccttgaac cgcaacgtgg
     3961 cggcgctctt tgaaggtcag gtggcgcctt tggaggttat caaatggaag gagatttacg
     4021 agcacgtgga ggcggccctc gacaagtgcg aggacatcgc ggtcatcatc gagagcgtgg
     4081 tggtgaagaa tgcgtgaggc gggcagaacc gaattggacc gaggatacaa tcccatacgg
     4141 cccgtatggc ctcggctaca ggggcaaaca tttttttgtg catacataaa gtcgttgccc
     4201 gcgggggaga gtggtcatga ttgagctttc ggcgggactg gtcgtcgtcc tcgtcctcgt
     4261 tctggccgcg gaatttgtca acggctggac cgacgcgccc aacgccatcg ccacggtggt
     4321 ctccacacgg gcactttcgc cggggaaagc ggtggttctg gcggcggtgc tcaatgccgt
     4381 cggagcgctc tccggaacgg cggtggcggc caccatcggc aaagggatcg tccgcccgga
     4441 ggtgattaac cttaccaccg tcggagcggc gatggtggcg attgtcttct ggagcacgct
     4501 cgcctggaag tacggcctgc cgacctccga aagccacgcc ctggtggcgg ctctggccgg
     4561 agcggggttg gccaccgccg gtccgtcggt cctgctctgg gtgggatgga aaaaggtgct
     4621 catcggtctc gtcttttcca cgtttttagg tttcggggtc ggctgggcaa tcgtcaaagt
     4681 cattttacgc ttctttgcgc agatgacgcc gaccagggcg cgcaaggtgt tcgggagaat
     4741 gcagttggtc tcggccgcct ttatggcttt cagccacggc agcaatgacg gccagaagtt
     4801 catcgggacg ttttcgctgg ctttagtgct gggaggagtt ctccccgaat tccgggtacc
     4861 ctgggaggtt attttgattt gctctctcat tatggggatc agcacgtcgg tgggcggctg
     4921 gcggatcatc aagacgatgg ggatgcagat ggtgaaatta gaaacctacc agggcttttc
     4981 cgccgaggcg ggagctgcct ccagtatcga actggcgtcg cgcctgggca taccggtgag
     5041 caccacgcat accatcggca cggccttaat gggcgtcggc gcttcccggc ggctttcggc
     5101 ggtgcgctgg ggcgtggcgg ggcaggtggt caccgcctgg attctgacct tcccggtctg
     5161 cggcttcatc gggtggctgg tggcgacagt attcgggatg ttctttcaca gcagatgaag
     5221 cagatgatca tggtgtcgga gtgtcgaggt gtcgaaagtg tcgaggttgt gattcgacag
     5281 acttaccaac agtaagtgtg aggtaagagc attcacagca gattgagcgg ataaaacaga
     5341 taagatcaaa tgccgggtaa aaacgagagg ggcaaacttt ccgggtggct ctccgtataa
     5401 aaggcgagaa agaaatggag gttggaaggt agatgaaagt tttcaagacg gtccttttgc
     5461 tcacggtgct gacgttgctt ttcgtcttca tcggcaacct gatcggcggg cagcagggga
     5521 tgctcatcgc tttgattttc gctttcgcaa tgaacttctt cgcctactgg ttctcggaca
     5581 aaatggttct ggcgatgtac cgggcgaagc ccgtcaccga gtcggataac ccccggctgt
     5641 accggattgt gcgcagcatc gcgacgaaag ccagcattcc gatgccgaag gtgtacacca
     5701 ttccggtcga tacgccgaac gcctttgcca ccgggcgcaa tccccagcat gcggcggtgg
     5761 cggcgacgga gggaattctc cgtttgctgg atgacaacga attagaggga gtgatcgccc
     5821 acgaggtatc gcacgtgcgc aaccgggaca tcctgatcgg caccattgcg gcgaccatcg
     5881 ccggagcggt gatgttcctt tcgcacttag cgcagtgggg cgcgatcttc ggcggcgggg
     5941 gcggacgcga cagcgagaac cggggcaatg cctttgctct tctggcggtg gcgattctcg
     6001 ctccttttgc ggcgatgtta gtgcagttgg cgatttcccg ctcgcgggag tatgcggcgg
     6061 atgagaccgg cgcgcgcatt tcgcggcagc ctttggctct ggcttcggcc ttaaagaaat
     6121 tgcagatctg caatgcccgc ctgccgatgc aggccaatcc ggccacggcg cacatgttca
     6181 tcgtcagtcc attctcggcg cgggggctgg cgaatctttt ctccacacac ccgccggtgg
     6241 aagagctggt ggcgcggtta gaaacgcttg cgagacagtt ttaattcctg aggagggatg
     6301 gggtggccaa aggcggcttg aaagagccgc ctttttattt ggaacaaaaa cgggggtcag
     6361 gcaagatgcc tgacctatgg ggctgaagag accgacggta taaacccaga ttccgagcga
     6421 ctggttcgat cgcgccgttg gcgggactca ggctaaaaag accggcacaa caagttctat
     6481 ctttccccgc gtcttgggcg actgaagtcg cccctcccga tgaaggagaa cacgtggtag
     6541 tcgggctcgt ccccgagccc gactactccc gctcaaggat gagcgggagt accggtcttc
     6601 caataatctt atggaatttt caaatagtat gttttgggtt cgccgcaaaa atcgtagtta
     6661 acttcttatc tctcaaagta ataggaaagt ctcgccagcc tttaatttcc cctttaacac
     6721 cattgtttcc ggcgcaccac ctgttagatt atccctcgga ggcccatgcc ggaatatctc
     6781 gtccagttaa ttctaatttt cgccctgatc ctggccaata gcttctttgc cgggtcggag
     6841 attgccttgg tctcggccag gcggagcaag atccggcaac tggccgaggc aggcgatacg
     6901 aacgccagaa tcgcggaagc actccagaac cagccggaga gactcttggc cacggtgcag
     6961 gtggggatga cggtggtttc gactctggcg ggggtgatcg ggggtgccac catcgtggcg
     7021 gtgctccaga aagagttggc ccaggtgccc gtcaccttca tccagaaggg gagccagacc
     7081 atcgccattg ccattgtggt agtatcgatt tcctacctca ccatcgtggt cggggagttg
     7141 gtgcccaagt atatcgccta cgtctattcc gaaacgctcg ctacccggct ggcccggccg
     7201 gtacactgga tctcccgcat tttctcgtgg gcgatcaaac tgctctcggt ctcctcccgc
     7261 ctcgtggtgc gggccatcgg gatcaagtcc cttccggaat caggggcggt ctccgaggaa
     7321 gagatcaaac tgcttttgcg cgaggggcag aagaagggaa ttttcgaaaa gaccgagcag
     7381 gagttgattc actcggtctt cgagttcacc gataccacgg tacggatggc gatgaccccg
     7441 cgcaccgaca tcgtcgccat cgacctcgcc tgggatcaga agcggctcct gcgcgccgtc
     7501 accgaggagg ggtactcgcg ctatccggtc taccagggat cgctggacaa cgtcgtgggg
     7561 atcatccaca ccaaggacat catcaatctt ttagtcgata gcgaactcat catcctgcag
     7621 gatatattgc gcaagccgta cttcgtgccc gattcgaaaa agatcggcga actcttgcgc
     7681 gacttccaga ggttgcaggt tcacctcgcc atcgtcttgg acgaattcgg cgggacggcg
     7741 gggttaatca cgctcgaaga catcttggaa gaaatcgtgg gcgaaattca ggacgagtac
     7801 gacatcgaag cggagcgcta cgtgcgcctg cccgacggct cggtgtgggt ggcggcgcgg
     7861 ctttcgctct ccgaactgcg cgagattctt cccggagagt tgccgccggg agccgatgcc
     7921 gacaccatcg gcgggctcgt cacaagcgcc ttaggccgga ttccatcgct ctccgaggcg
     7981 gtgaacgccg gggattatcg ttttaccatc atggagaaag cggggcaccg gattacacgg
     8041 gtgcgggtgg agaaaattca gaacacggca taccggacac agaatacagg ggagaatcat
     8101 tgacccaact tctcattccc agttcccaac aaatccgaaa gtctggcgtt ccccggcaat
     8161 ggtatctaaa atcttagagg cggtgggggg gtggatcgtg gctttgattt cctcctccgg
     8221 ctacgcggga gtggtcatcg ccatgaccat tgagtcggcc tgcattccgc tcccttccga
     8281 aatcatcatg ccctttgcgg ggtatctggt cttcaccggt cggttcacta tgctcggggc
     8341 gtcggtggca ggggcgttcg ggtgtctggt cggctccatt ctggcctacg gcattggcta
     8401 ctacggcggc aggcctttgg ccgtgaagta cgggcgctac gttttgctct cccaacacga
     8461 tctggacaaa gccgaccggt ggttcgcccg ctggggcaac
//
LOCUS       DBNGZD010000136         1384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_995740, whole genome shotgun sequence.
ACCESSION   DBNGZD010000136 DBNGZD010000000
VERSION     DBNGZD010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_995740"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1384)
                     /locus_tag="AC16GM_03730"
     CDS             complement(<1..>1384)
                     /locus_tag="AC16GM_03730"
                     /inference="COORDINATES: protein motif:HMM:NF017043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HIR2922819.1"
                     /translation="YPSSDGFGLAQLEVSTGEFRVEEGKPERINELLSVLAPAEILLP
                     SSAKEHQITRNHFPWQPQFSYLDDWKFAFDFAYRSLTEHFGTQSLAGFGCDGLKVGIS
                     AAGATIVYLKETKKTVLSHIRSLSRIGSQSEMFLDFATVRNLELVSSLGSDERKYSLF
                     GVLNRTRTAMGARLLRQWLLAPLIEVEPIQKRQKAVARLVKDADLRGDIQNLLSRASD
                     LERLAGRLGASKASPKDLIGIKETCALLPTFQKLLADEKVSLLAELNQELPDVSDVVD
                     LISRSVVDEPPVVTNEGGLIKSGYDGKLDSLKDSIRGSKTWIANLQAIERERTGIPSL
                     KVGFNKVFGYYLEVTTPHVAKVPPDYIRKQTLVNGERYITPELKEKEELILNAEEKIN
                     RLEQELFLVICQRVAQRIGDLQRAASVLAQLDVLASLAQVGWENGYCLPEVNTEGQIE
                     ITEGRHPIIEQ"
BASE COUNT          300 a          379 c          377 g          328 t
ORIGIN      
        1 cttgttcgat gatggggtgc cggccttcgg tgatttctat ctggccctcg gtgttgacct
       61 cgggtaaaca gtagccgttt tcccacccga cttgggcgag agaggcgagt acatccagct
      121 gcgccaggac cgaggccgcc ctttggagat cgccgatgcg ctgggcgacg cgctggcaga
      181 tgaccagaaa cagttcctgc tcgaggcggt tgattttttc ttcggcgttg agaatcagtt
      241 cttctttctc ttttaattcc ggggtgatgt accgctcgcc attcaccagc gtctgcttgc
      301 ggatgtaatc cggcgggact ttcgccacgt gcggggtggt gacctccaga tagtagccga
      361 aaaccttgtt aaacccgacc tttaatgaag gtatgccagt cctttcccgc tcaatggctt
      421 gtaaatttgc aatccaggtt ttactgcccc ggatagagtc cttcagagaa tcgagtttgc
      481 cgtcgtagcc ggacttgatc agcccgccct cgttggtcac taccggcggt tcatcgacaa
      541 cgcttcggga gatgagatcg acgacatcgc tgacgtccgg gagttcttga ttgagttcag
      601 cgaggagtga gactttttcg tctgccagca atttctgaaa cgtaggcaaa agtgcacagg
      661 tttctttgat gccgatcaaa tctttcggac tggccttgct cgctcccaat cgtcctgcta
      721 acctttccag atccgaagcg cggctcaaaa gattctggat gtctccgcgc aggtcggcat
      781 ccttcaccaa acgagcgacg gctttctgcc gtttttgaat cggctcgact tcgatcaaag
      841 gcgcaaggag ccattggcgc agcagccgcg cacccatggc ggtccgggtg cggttcaaaa
      901 ccccgaaaag tgagtatttc cgctcatcac tgcccagcga tgacaccagt tcgagattac
      961 gcaccgtggc gaaatcgaga aacatttctg actgcgagcc gatgcgggaa agcgagcgaa
     1021 tgtgggagag gacggttttt ttggtttctt tgagataaac gatagtcgct cctgccgcag
     1081 aaattcccac tttcaggccg tcacagccaa aaccggccag agactgcgtc ccgaagtgct
     1141 cggtcaaact gcggtaggcg aaatcaaaag cgaatttcca atcgtcaaga tacgaaaact
     1201 gcggctgcca ggggaagtga ttcctggtaa tctgatgttc tttggcagaa gacggcaaaa
     1261 gaatttccgc cggagccagg acggagagga gttcattgat ccgctccggc ttcccctcct
     1321 ccacccggaa ctcccctgtg ctcacttcca attgcgctaa gccgaaacca tcggaagacg
     1381 gata
//
LOCUS       DBNGZD010000137         8375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_301486, whole genome shotgun sequence.
ACCESSION   DBNGZD010000137 DBNGZD010000000
VERSION     DBNGZD010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_301486"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="AC16GM_03735"
     CDS             complement(<1..1399)
                     /locus_tag="AC16GM_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922820.1"
                     /translation="MAQDVFKILKDLELKAVGLDPQTNKMQEGYFVSFRTVGLPIHKD
                     DFNNPYAPTGVNLQKDIPKSDPVDPANAPTTASGKIDEDKIFTANIAKSMQSYLNTFL
                     LTDDKLRMNNQYAVMPGSSKVSDSWYAITSGANGIPTTSELSDAMKKAYDDARAKLMD
                     KDGNPTPHYEAYIRYEDEYNSKRRTYFKAYADAFTNHMKLQNFPNEGKLYHDDVDEAW
                     NRWQGLGFKEEIEKAINTLAAQGTDPAIALIARAKKRFINSLVEFMNIGEIPYTVMLP
                     NSWYEVDNDDGWNDYTEHDFHSESHYVASSTSIGVSGGFNIGLFSAGASFNHNESKAS
                     SNFQMNNLRIRVKYCVVDIIRPWLDTSLLNLKNWFLMGEYKKNCISNGTMGQELPQGN
                     PEPPFLPSLVTSLILIKDLEIYWDNWKSDWQTFQQSFRKFKYVMVPVDYSKCKRTVLP
                     VSKKDFFENVELCKET"
     gene            complement(1513..2499)
                     /locus_tag="AC16GM_03740"
     CDS             complement(1513..2499)
                     /locus_tag="AC16GM_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR2922821.1"
                     /translation="MAVFWGCHRAGCNNEMDGMTKRSLVIGIDDYSGIDPSGKSNLGS
                     CVADANNVYELLISAFGFDPDQSTKFTDQQASSSAIQLSLSNLIAKSGPGDVICFYYS
                     GHGSTAAANPNQDCEKFFQTIVPASGRQISDATLFKIASKLQQSVVNFTVILDSCHSG
                     GMDQETDAVLRCKSLKLSDEIVERIQEFLHTLIPCGICIPASADVCDNNVSNVSVSNG
                     CFSLDEDSDKVFVPLAKTTLIAGCKFNELSWETGGHGLLTKSLLDLVNASNFQISYRD
                     ILDELETRVSDYFNTLILPTIPHNLPRSQTPQLRGQANRMDEGFLQGWMDSS"
     gene            complement(2916..4373)
                     /locus_tag="AC16GM_03745"
     CDS             complement(2916..4373)
                     /locus_tag="AC16GM_03745"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR2922822.1"
                     /translation="MSASTKKILVVDDDPEVAASVADLFDPQEFSCQTAGQASSAFQK
                     LSNENFDLLLLDVNLPDRSGLDILQEIKAKHHDLPVVVITGFVSAEGALQAMKDGAYE
                     FLTKPFTAQKLSEVVKRALGQPQKKGKAAGTDWESAQEFTEDALIGKSPEIVEIAKLI
                     GQVAQSDAAVLITGESGTGKELVAQAIHKNSKRCDKAFLSVNCAALPETLLESEMFGH
                     EKGAFTGAYSRKPGKFEQCHTCTIFLDEVADMSLLTQSKVLRVLQEQAFERVGGDQTI
                     KVDVRIIAATNKSLVQCMRENQFRVDLFYRLKVVSLYLPPLRERKVDIPLLAGHFLRR
                     YARELNKPVQGFAPDVQAALASYHWPGNVRELENNIHTAVVLAKEDILTLEHFPFYSE
                     NKNQPQPTFDELRDDYSKVFTNFIDARIGHLVAGANNRLYHQLEDALEKSLITAALRH
                     FNSNQSKTAEVLGLSRNTLRDRMQRFGLFGQAIQE"
     gene            complement(4508..5746)
                     /locus_tag="AC16GM_03750"
     CDS             complement(4508..5746)
                     /locus_tag="AC16GM_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR2922823.1"
                     /translation="MASEFEIVDRFLKRPAPQKNNSFARIARELGGQLHETPAGPVIH
                     LASKTSPTDRYGRLRLKEIWREDASALGGLEFLVKEPPPGNVALEDLLFLDCETTGLA
                     TAAGTYAFLVGLGAFRGQTFHLEQIFLPDYSCEKAFLQTLLKKIRPGTVLVTYNGKSF
                     DVPLLQTRFILNRLAFPLAELPHVDLLFPARRIWRYRLADLSLRSLERELLGAERERD
                     IDSGLIPGTYFHYLRSGDVSYLPDVLAHNRYDILTLAFLTLWHASLLATPQGYPNLEP
                     CDYFGLGRLYYQKHEVEKAVWCLEQAWGKAEDETLKGLAGTLLSLIHKRRENWPEATN
                     LWQAMSRLPLRWALYATEELAKHYEHRARDYSQALRMVENALATFAFSEPEHIHFPLE
                     RWEKRKVRLLCKQKRPRQLY"
     gene            complement(5764..6777)
                     /locus_tag="AC16GM_03755"
     CDS             complement(5764..6777)
                     /locus_tag="AC16GM_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2922824.1"
                     /translation="MLGQPPFQIRERPTLVGLRLHRIKQLFSPRVDGSLVPLLWVWLA
                     FASHLQADDSLAKRLDSLWIRASSGEERFRDQVEPCKQDLIAMGDTAVPYLVTKLSVE
                     DAREALTLELIFGGIGAPAVPALCAALSDSNIWVLRNIGRALAAIKDKRAVGPLIGIL
                     KNPEQGVRSQACTALGKIADTSATLALLPMLADSAETVRKSASVALGGLADLRGLLAL
                     VAALGDSYYGVRFTAMEGLVKIGKPAVEPLLKFLDSAPPKARWLAIATLGKIKDPHAE
                     GKLTAELKSTDPVSRAFAVDALVDLGTPGSRVALQKLSDPDPFVQSRLAKAKEKMIAS
                     PKK"
     gene            complement(6837..8003)
                     /locus_tag="AC16GM_03760"
     CDS             complement(6837..8003)
                     /locus_tag="AC16GM_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922825.1"
                     /translation="MRDGLLLDLDTHRGKIAIGGTGPNTYKGTYQLIIDLGGDDRYEL
                     EGGPGQNQIIIDLGGNDTYVAKSDYAIASGFMGVGILIDESGDDTYIGKNFSLGSGLF
                     GVGILWDKAGNDKYYGDQFTQGAGSFGLGALIDDGGNDQYHAAMSAQGFGFVKGAGIL
                     ADRSGNDTYFAGGKYQDVLRYKDHYLSLSQGFAYGYRPTLSGGIGLLFDGQGNDLYVT
                     DIFGQGASYWWGLGGLVDLQGNDQYISFQYAQGSAEHMTLGVLIDSSGDDLYRSKGVS
                     QGCGHDYAAGMLLDYGGNDTYIAQDLSQGAGSANGIGILIDKSGDDRYYVGDTTNTQG
                     YGNPRREYGSIGVLLDCLGKDRYDGNGKDNDVWIINSTWGVGVDGEFQQVVGGR"
BASE COUNT         1893 a         2178 c         2206 g         2098 t
ORIGIN      
        1 ttgtttcctt gcatagctca acgttttcga agaaatcttt tttagaaacc ggaagcaccg
       61 ttcttttgca tttggagtaa tcaacgggca ccattacgta tttgaatttc cggaacgatt
      121 gctggaacgt ttgccagtct gacttccagt tatcccaata aatctctaag tccttaatta
      181 atatcaaact ggtaactaag gacgggagga acggaggctc cggattgcct tgtggtaatt
      241 cctgtcccat ggttccattg gaaatgcagt tctttttata ctctcccatc agaaaccagt
      301 ttttcaaatt aagaagtgag gtatctaacc aaggccttat tatgtctact acacaatatt
      361 taaccctgat tctcaggtta ttcatttgaa aattggaact ggccttcgac tcattatgat
      421 taaatgaggc accggcagaa aagaggccga tattaaatcc gccgctgacg ccaatcgaag
      481 ttgaggatgc cacgtagtgg ctttcggagt gaaaatcgtg ctccgtgtaa tcattccatc
      541 catcatcatt gtcgacctcg taccaagagt tgggaagcat taccgtataa ggaatttccc
      601 caatattcat gaattccaca aggctgttaa tgaatctctt tttagcccgc gctatgagcg
      661 caatagccgg atccgtcccc tgagctgcta gggtgttaat tgctttctca atttcttctt
      721 taaatccaag cccttgccat ctattccatg cttcgtctac atcgtcatgg tacagtttgc
      781 cctcatttgg aaaattctgt aatttcatat gattagtgaa agcatcggca taggccttga
      841 aatatgttct tcttttgcta ttgtactcat cttcatagcg tatgtatgcc tcgtaatgcg
      901 gtgtagggtt tccatccttg tccattaact tcgctcttgc atcatcgtag gcttttttca
      961 tggcatcact taattccgag gtcgtaggta tgccgttagc cccagaagta attgcgtacc
     1021 atgagtcact cactttgctt gatcccggca tcacagcata ctgattattc atcctcaact
     1081 tatcatcggt aaggagaaaa gtgtttaagt agctctgcat ggatttggct atgttggccg
     1141 tgaatatttt atcttcatcg attttcccgc ttgctgttgt gggcgcgttt gcaggatcaa
     1201 cgggatcact tttcgggata tctttttgaa gatttacacc tgtaggtgcg tatggattat
     1261 taaagtcatc cttgtgaatt ggaagaccga cagttcggaa ggatacgaaa tagccttcct
     1321 gcattttgtt tgtttgagga tcaagcccaa ccgctttgag ctctaagtct ttcaagattt
     1381 taaaaacatc ttgtgccata atcttaatct cctttctaaa agtttccgaa tgtcagttta
     1441 tttggtttcc tttcacggat ctaacaagac cgtctaatct caacgggatg cccaattcct
     1501 ttctttcacg gtttagctgg agtccatcca gccctgtaga aatccttcgt ccatacgatt
     1561 cgcttgacct cgcagttgag gtgtctggct cctgggaagg ttatgtggaa tcgtcggaag
     1621 aattaaggta ttgaaataat cgctaactct tgtttccaac tcgtctagga tgtctcgata
     1681 acttatctgg aaattgcttg cgttcacaag atctagcaaa gactttgtca gcagtccatg
     1741 gccacccgtt tcccacgaca gctcgttaaa tttacatccg gcaatgagcg tggtctttgc
     1801 cagtggcaca aacactttat ctgagtcctc atcaagactg aaacaaccat tgctcaccga
     1861 aacattgcta acgttattgt cacagacatc ggcacttgct ggaatgcaga ttccacacgg
     1921 gattaaagta tgcaagaatt cctgaatacg ttcaactatc tcatcagaaa gcttcaacga
     1981 cttgcatctc agtaccgcgt cagtttcttg atccatgcca cctgaatgac aagagtctag
     2041 aatgaccgtg aagttaacta cagattgttg aagcttactg gcgattttga aaagcgttgc
     2101 atctgagatt tgacgtcccg atgccggaac gatggtttgg aaaaattttt cgcaatcctg
     2161 atttggattg gcggcggcag ttgaaccatg tccggaataa taaaagcaaa taacatcacc
     2221 gggcccggac ttggcaatga ggttgctcag tgataattgg atagcgcttg aactagcttg
     2281 ttgatccgtg aactttgtcg actgatcggg atcgaatcca aaggcactga tcaataactc
     2341 gtagacattg ttggcgtccg ccacacatga gcccaagttt gacttgccac tggggtcgat
     2401 gcccgaataa tcgtctattc ctataaccaa tgatcttttc gtcataccgt ccatttcatt
     2461 gttacaacca gctcgatgac acccccagaa aacagccacc tcctgctcaa cgtccccacg
     2521 cttcctcgaa cccctctgga caggcatcgt tgctctttcc atctgacatc ctcgtccaat
     2581 aatacctgtc ccctccttaa aaaaaattaa attaaataca aacgctcttg atgtcaagaa
     2641 atctccctga ttttttcaac cctttaatct taagtaagtt aagaagttag ctgaaataag
     2701 caaataaagg tgaaactttt cacttttatc catattcaac gcatcaaaat cgcacaagca
     2761 atttactcaa cgggcctaat attgtgaaat tctatactat ttagagtagg aatgacttat
     2821 gtgtgacctc acgttggacc tcagaatctg cgagaaaaat tggagttgtg attaggaact
     2881 tatttgtaac ggcgagccgt ccattcgctc gatttttatt cctgaatggc ctgcccgaac
     2941 agcccgaagc gctgcatccg gtcgcgcagg gtgttgcggg aaagccccag cacttccgcc
     3001 gttttcgact ggttgctgtt gaagtgccgg agcgcggcgg tgatgagcga tttttccagc
     3061 gcatcttcca actgatggta tagccgattg ttggcgcccg ccaccaggtg gccgatgcgg
     3121 gcgtcgatga agttggtaaa aactttgctg tagtcgtcgc gcagttcgtc aaaagtcggc
     3181 tggggctggt ttttgttctc ggagtagaag gggaaatgtt ccaaagtcaa aatatcttcc
     3241 ttggccagca cgacggcggt gtggatgtta ttttccagtt cccgcacgtt gccgggccag
     3301 tggtagctcg cgagcgcggc ctgcacgtcc ggggcaaagc cctgcaccgg tttattcagc
     3361 tcccgggcgt agcggcgcag gaagtgaccc gccagaaggg ggatgtcgac cttccgttcg
     3421 cgcaagggcg gcagatagag ggagacgact ttgagccggt agaagaggtc gacccggaac
     3481 tggttctccc gcatgcactg gaccaaactc ttgttggtgg ccgcgatgat gcgcacgtcg
     3541 accttgatgg tttggtcgcc gcccacccgc tcgaaggcct gttcctgcag aacccgcagg
     3601 accttgctct gggtcaaaag cgacatgtcg gccacttcgt ccaagaaaat cgtgcaggtg
     3661 tggcactgtt cgaacttccc cggtttgcgg gaataagcgc cggtaaaggc gcccttctcg
     3721 tgcccgaaca tctcgctttc cagaagcgtc tccggcaggg cggcgcagtt gaccgagagg
     3781 aacgctttgt cgcagcgttt gctgttctta tggatggcct gagccaccaa ttccttgccg
     3841 gtgccgcttt cgcccgtgat gaggacggcg gcgtccgact gggccacctg gccgatcaac
     3901 ttggcgattt ccacgatttc cggactcttg ccgattaaag cgtcttccgt gaattcctgg
     3961 gcactctccc aatcggtgcc ggccgctttc cctttcttct ggggctgccc tagggcgcgc
     4021 ttgaccactt cggaaagttt ctgcgcggta aagggcttgg tcaagaactc gtacgcacca
     4081 tccttcatgg cctggagggc accctcggcg gagacgaacc ccgtaatcac caccaccggc
     4141 aggtcgtgat gcttggcctt gatttcctgc agaatgtcca acccggagcg gtcggggagg
     4201 tttacgtcca gaagcaggag gtcaaaattt tcatttgaaa gtttttggaa ggcgctggag
     4261 gcctgcccgg cggtttgaca gctaaactcc tggggatcaa agagatcggc gacggatgcg
     4321 gccacctccg ggtcgtcatc cacgaccagt atctttttcg tcgacgcact catagcgaga
     4381 atccggcttc tctatcatat catcggtaga aataaggcag ttcattacct gccgaaacgg
     4441 ggcaaaatta ttttaaatga gactgtaata ttatggagaa ataaaaaaca aaatctggcg
     4501 aattatctca gtagagctgg cggggccgtt tttgtttaca gaggaggcgg acttttcttt
     4561 tttcccagcg ttcgagaggg aaatggatat gctccggttc agaaaacgca aaggttgcca
     4621 gggcattttc aaccatccgg agcgcctgtg agtagtcgcg cgcgcggtgt tcgtagtgtt
     4681 tggccagttc ttctgtggca tagagcgccc agcggagcgg caggcgggac atagcctgcc
     4741 agaggttcgt cgcttccggc caattctccc ggcgcttgtg aatcaagctc aggagcgtcc
     4801 cggccaagcc cttgagcgtt tcatcttccg cttttcccca cgcttgttcc aagcaccaga
     4861 ctgctttttc gacttcgtgt ttttgatagt acagccggcc cagcccaaaa tagtcgcagg
     4921 gttccaagtt tggataacct tgcggcgtcg ccaggaggct cgcgtgccaa agcgttagga
     4981 acgccagagt cagaatatcg tagcggttgt gcgccagaac gtcggggagg taactcacgt
     5041 cgcctgagcg caggtagtgg aagtaggtgc cgggaatcaa gcccgaatcg atgtcccgct
     5101 ctcgctcggc gcccaaaagt tcccgttcca gcgaacgcag ggagagatcg gccagccggt
     5161 aacgccaaat gcgccgggca gggaagagga gatcaacgtg cggcaactcc gccagaggaa
     5221 aggccaaacg gttgagaatg aaacgggttt gcaaaagcgg aacatcgaaa cttttgccgt
     5281 tataggtcac caggacggtg cccggcctta tttttttcag gagtgtttgg agaaaggctt
     5341 tttcgcaact gtaatcgggc aggaaaatct gttcgaggtg gaaggtttgt ccgcggaaag
     5401 cgcccagtcc gaccagaaag gcataggtgc ccgccgcagt agccaggccg gtggtttcgc
     5461 agtctaaaaa gagcaggtct tcgagggcaa cgttccccgg tggaggctct ttcacaagaa
     5521 attccaatcc gcctaaggcg gacgcatctt cgcgccagat ctcttttagc cgcaagcgtc
     5581 cgtagcggtc agttgggctt gttttcgaag caaggtggat gaccggcccg gccggggtct
     5641 cgtggagttg cccgcccaac tctcgggcga tgcgggcgaa agaattattt ttctgtggag
     5701 ccggcctctt cagaaaccgg tcaacaattt cgaattcact tgccattgga gataagatcg
     5761 caactacttc ttcggggacg caatcatttt ttctttcgct ttggccagac gggactgcac
     5821 gaaggggtcg gggtcgctta acttctggag agcgacccga ctcccgggcg ttccgaggtc
     5881 gactaaagca tccacggcaa aagcgcggga aacgggatcg gtcgatttca attccgctgt
     5941 cagtttccct tcagcgtgcg ggtctttgat ttttcccaaa gttgcgatag ccagccagcg
     6001 ggctttgggc ggggccgaat ccaagaactt gagcagcggt tcgactgccg gtttgccaat
     6061 cttgaccaag ccctccatag cggtgaagcg tacgccgtag tacgaatcgc cgagggcggc
     6121 caccagcgcc aggagcccgc gcaggtcggc aagccccccc agcgcgaccg aagcgctctt
     6181 gcgcaccgtt tccgctgaat ccgcaagcat aggcaaaagc gcgagggtgg ccgacgtatc
     6241 ggcaattttg cccagagcgg tgcaggcctg gctgcgcact ccctgttccg gatttttcag
     6301 aatgccgatg agcggcccga cggcgcgttt gtctttgatg gcggccaagg cccggccgat
     6361 gttgcgcagc acccagatat ttgagtccga gagcgccgcg cacaaagccg gcaccgccgg
     6421 cgcgccgatg ccgccgaaaa tcagttccag ggtcaaggcc tcccgggcgt cttcaacgga
     6481 aagtttggtg acgaggtagg gcaccgccgt atcgcccatt gcgattaagt cctgtttgca
     6541 cggttcgacc tgatcgcgga agcgctcctc gccggaggag gcgcggatcc agagggagtc
     6601 gaggcgtttg gcaagcgagt cgtcggcctg tagatgggag gcaaaggcga gccaaaccca
     6661 aaggagcggc actaaagagc cgtctacgcg gggggagaaa agttgtttta ttctatgcag
     6721 tcgtaggccg actaaagtcg gccgctcccg gatttgaaag gggggctgtc caagcattgt
     6781 ggattttcct ttactcccat ctcccaactc ccaaatccgt ccttccctac cacctatcac
     6841 cttccaccta ccacctgctg aaactctcca tcgacgccga ccccccaagt ggaattgata
     6901 atccagacgt cattgtcctt gccgttgccg tcgtagcggt ctttgcccag gcagtcgagg
     6961 aggacgccaa tggagccgta ctcccggcgc ggattgccgt agccctgggt gttggtggtg
     7021 tcgccgacgt agtaacggtc gtcgccagac ttgtcgatga ggatgccgat gccgttggcc
     7081 gagccggcgc cctgcgagag gtcttgggcg atgtaggtgt cgttgccgcc gtagtccaaa
     7141 agcattccgg cggcgtagtc gtggccgcag ccctgcgaca cgcctttgga gcggtagaga
     7201 tcgtctccgg aggaatcgat caagacgccc agggtcatat gctcggctga gccctgggca
     7261 tactggaacg aaatgtactg gtcgttcccc tgcaggtcga ccaacccgcc caacccccac
     7321 cagtaactgg cgccctggcc gaagatgtcg gtgacgtaga ggtcgttgcc ctgcccgtcg
     7381 aacaataacc cgataccgcc ggagagggtg gggcggtagc cgtaggcgaa gccctgagaa
     7441 agggagaggt agtgatcttt gtagcgcaag acgtcctggt acttgccgcc ggcaaagtag
     7501 gtgtcgttgc cggagcggtc ggctaaaatt cccgcgcctt tgacaaagcc aaagccctgc
     7561 gccgacatgg cggcgtggta ctggtcattt cccccatcgt caatcaacgc cccgaggccg
     7621 aaggagcccg cgccctgggt gaactggtcg ccgtagtatt tgtcgttgcc cgccttatcc
     7681 cacaggatgc cgacgccgaa aagcccggag ccgagggaga agtttttgcc gatgtaggta
     7741 tcatcacccg attcgtcgat taaaatcccc acgcccataa agccggaggc gatagcatag
     7801 tcgcttttgg ctacgtaagt gtcgttgccg cccaggtcaa taatgatctg attctgacca
     7861 ggcccgcctt ctaattcgta gcggtcgtcg ccgccgagat caatgatgag ttgataggtg
     7921 cctttgtaag tattcggtcc ggttccacca atggcaatct tacctcgatg agtatcgagg
     7981 tcgagcaaca atccatctcg cattttgcct ttatcaagat tctcgtgaga tgaatacaac
     8041 tgactgagcg cttcgcctgt tgcgaccgaa acccttcttc ctaaactgag catgagtttg
     8101 ttgtcaattt ttcgccctct ggacgggaat tctttagccc attggtcttt tcgtttctgg
     8161 aggctgtcaa tttcttcaac agaacgccgt tcgtcggaag tatcttcgag aagtagttgg
     8221 ggaaaccgga aggtagcaaa atttctatcg gagctgtcgg ggatcaactg tatagaagaa
     8281 aaaagatcga gcgctctcag cattgtccgt aagcttgttt ttagagattc gttgagcgac
     8341 ccgacgcggt cgatactttt tcgaacgtgg cgagg
//
LOCUS       DBNGZD010000138         8239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1135176, whole genome shotgun sequence.
ACCESSION   DBNGZD010000138 DBNGZD010000000
VERSION     DBNGZD010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1135176"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="AC16GM_03765"
     CDS             <1..1272
                     /locus_tag="AC16GM_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HIR2922826.1"
                     /translation="NGPGTKPPAKNRAVADCYEPGSTFKIVTAAAALEEGVKKPEGQI
                     FAENGAWRLGRRVIHDAEKHGWLSFREAVVHSSNIALAKVALEVGGPISYRYAHDFGF
                     GEKTKVDLPGEGRGYLPPQKIWETSQLANFAFGQGVSVNALQLVMAYACIANDGLLLR
                     PYVVKEIGEDGRDTGEKRLPQVVRQVVSVATARTLCQWLAEAVRDGTGRAATVEGLEV
                     AGKTGTAEKPNLERGGYFKNRYVSSFVGFFPADQPRVVGFVAIDDPYGLHFGGQVAAP
                     IFQRVAQRVLPLLPAPPPQLIQPSLVENSGSDAGASLSVNQQPAREENGEPFFAAQAA
                     RAEEAAPKITTPASLIGPPVPEDLAPSATVPDLAGLTIREAVGVLGRFGFRARVRGSG
                     VVVAQSPAPGPVRGNTKTVIITGLPPVPRAW"
     gene            1284..2735
                     /locus_tag="AC16GM_03770"
     CDS             1284..2735
                     /locus_tag="AC16GM_03770"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR2922827.1"
                     /translation="MISALEEKEVRGDVRVAISGLEYDSRNIKRDNLFVALSGFALDG
                     HRFAPEAVARGASSLVVERWLNTLPRPQVRVPNSRAALARLAHQFYGHPGEKLTVIGV
                     TGTNGKTTTAYLVKKILETAGEKVGLIGTIQYEIAGEALPAWNTTPESLDLARFFVRL
                     QEAGAKSCVMEVSSHALALHRVEAIDFRVAVFTNLTQDHLDYHRTMENYREAKAKLFR
                     SLQGKDRWAAINRDDPESAYFEQQSGGSVFTYSLEGRGADLIATRIQPSENGSRFELK
                     TDAGTYAVNFPLVGRFNVANALAAAAGAHLVGIGWETVAVGLEKIEPVPGRVEPVQSG
                     QPFTVVIDYAHTPDGLKQILTLARSQSKAGVIVVFGCGGDRDRTKRPLMGEIAGALAD
                     RVIVTSDNPRDEDPQKIIDEVLPGLRGKKNVRTSVDRAEAIADALHAARRGDWVVIAG
                     KGHEEYQIIGKEKIPFSDREEVKRVLGTMGFRG"
     gene            2741..4192
                     /locus_tag="AC16GM_03775"
     CDS             2741..4192
                     /locus_tag="AC16GM_03775"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR2922828.1"
                     /translation="MMEVISLAPVQTMFPITLDDVMAATGGRLLLPGLNGTAGITGVS
                     TDSRTLKPGELFVALSGERFDGHTFIGEAVGRQAGALLVQHDHEAHLHLASLSLPVLS
                     VPSTLAALGDLGAWFRRKFPLPVVAVTGSVGKTTTKEMSACVLETKYRVHRSAGNYNN
                     LVGIPLSLFALKPDHQVSVLELGMSTPGEIGRLTEITRPEAGVITNIAPAHLETMGSL
                     ENIARAKFELLDGLGETKTVILNADDPFLAARARQERRPVVTFGIQKPADIWAGDRAP
                     DENGCYRFRVNDQTDVRLAVLGLGNVYNALATLAVATRFGITFEKAAKALENYRPAKM
                     RLEVFTVAGVKIIDDSYNANPYSLRCALETLGQMNPPKRDSGRRVAVLADMLELGEKQ
                     ETLHREVGSVAAKAGVDFLIAVGPLAGEYLAGATPVGMFSSQMRHFENNPAALDFLLG
                     WLRAGDLVLVKGSRGMKADAIVTGLKELLPKQS"
     gene            4200..5285
                     /gene="mraY"
                     /locus_tag="AC16GM_03780"
     CDS             4200..5285
                     /gene="mraY"
                     /locus_tag="AC16GM_03780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR2922829.1"
                     /translation="MLYHLFYPLSQYISGFNLFRYITFRTAGATVTALFIAWVIGPYI
                     IRKLRARQVGEKIRAEGPQTHLKKEGTPTMGGIIILAAVVVPTLLWADLSNRFVQVAL
                     LVTVWMGVVGFVDDYLKVIKGKRKGLVARNKFSGQILLGLIVGAILYFFPPAKEFTPD
                     TDIPFFKNYYIPFGALYIPFVVLIITGTSNAVNLSDGLDGLAIGLVGICFFAFAGLAY
                     LTGRVDFSHYLTITYLEGAGELTIFCGAVLGAALGFLWFNAHPAEIFMGDTGALALGA
                     ALGTVAVLIKKEILLVIVGGVFVAEAASVILQVVSFKTRGKRIFKMAPLHHHFELLGW
                     AEPKVVVRFWIAGALLALLTLSTLKIR"
     gene            5285..6736
                     /gene="murD"
                     /locus_tag="AC16GM_03785"
     CDS             5285..6736
                     /gene="murD"
                     /locus_tag="AC16GM_03785"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR2922830.1"
                     /translation="MTELFSAAAPAANQSNFNGQGNHFRPDRVTGKRVGIVGMARSGL
                     ACARLLANQGAAVFLSDTKSAGQLQTEISQLQKWGLEYEAGGHTRKLVTDRDFLVLSP
                     GVPPDVPICRAAEAQGIPVFSEIEVASWLCPAAIVGITGSNGKTTTTSLAGEMFRNAG
                     YHTLVAGNIGTAFSSVVEQATKASVVVLEISSFQLEKIEEFRPRTAVLLNLSPDHLDR
                     YGNYEAYKQAKLRIFENQKETDFAVLNADDLGLSDLPPKLAAKVAFFSTKGEVGFGAC
                     IRQGEICLKRNGHYDTVLPAAKVGIPGPHNLSNATAACAIGLSFGLAPEVMARALAEF
                     KGVEHRLEFVAEIDGVKFINDSKATNVDAVWYALQSVPQPIVLIAGGKDKGGSYAPLR
                     EFVAQRVKKLILIGQAKEKIKEALGDVVTTVTAESLPEAVEVGFSSAAPGDTVLLSPA
                     CASFDMFTNFEERGRIFKQTVAGLKGRTGGGDQ"
     gene            6733..7839
                     /gene="ftsW"
                     /locus_tag="AC16GM_03790"
     CDS             6733..7839
                     /gene="ftsW"
                     /locus_tag="AC16GM_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR2922831.1"
                     /translation="MSRRIDFFLLLIILSLLAIGTVTVYSSSAYLGEVRYRNPAVYFH
                     RQVIWAGLALVTLWFFSRIDYHRWRPLAPWGLAGGILLLIAVLFFPAKNDAHRWIDFG
                     PIQFQPSELFKLAVILYVAAALAKRGHKISDLRYLLLPYAPLLGVAFILLMLEPDLGT
                     FLVIAGIVFSSLAIAGARWKHLLTGFLAAALVVSVLVVGLGYKQQRVKDYVKSWEDPL
                     AGSYQVKQAMLGLSLGGLTGAGLGEGRQKMLYLPAPHTDFIFAAIGEEGGLLWLALIL
                     ALFFVLIWRGWLVAQQAPDRFGFSLAFGITALLFTSITLNVGVVLGLLPTTGIPLPFL
                     SYGGTALLAASAGCGVLLNIASQKEMQARYGYVR"
     gene            7823..>8239
                     /locus_tag="AC16GM_03795"
     CDS             7823..>8239
                     /locus_tag="AC16GM_03795"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR2922832.1"
                     /translation="MATYGKLKILFAGGGTGGHLFPAIALAQAAEKAFPGVQVVFVGA
                     PQGLEAKKIPELGWRLHLVPIRGLSRTRPFSNLVFPFSVVRSLVQANRVLSLEQPDLV
                     VGSGGYVAWPVLRAAVAKSIPTLIQEQNSYPGIVTRR"
BASE COUNT         1562 a         2365 c         2595 g         1717 t
ORIGIN      
        1 aacggtccag gaaccaagcc gccggcgaag aaccgggcgg tggccgactg ctacgaaccg
       61 ggatcgacgt ttaagatcgt aacggcggcg gccgctttag aagaaggagt gaaaaaaccc
      121 gagggacaga tctttgccga aaatggcgcc tggcggctcg ggcggcgggt gattcacgac
      181 gccgaaaagc acggatggct ctcgttccgc gaggcggtgg tgcactcctc caacattgcc
      241 ctggccaagg tggcgctgga ggtgggcggg ccgatctctt accgctacgc ccacgacttc
      301 ggtttcggcg aaaaaacgaa agtggacctg cccggcgaag gccggggcta cctgccgccg
      361 caaaaaatct gggaaacgtc gcaattggcc aattttgctt ttgggcaggg ggtgtcggtc
      421 aacgcgctgc aattggtaat ggcctacgcc tgcatcgcta atgacggcct gctcctgcgt
      481 ccgtacgtcg tcaaggaaat tggggaagat ggccgggaca ccggcgaaaa acgcctgccc
      541 caggtggtgc gtcaggtggt gagtgtggcg acggcccgaa ccctgtgcca gtggctggcc
      601 gaagcggtgc gggacggcac cgggcgggcc gccacggtcg aagggctgga ggtcgccggc
      661 aagaccggca cggcggaaaa accgaattta gagcggggcg ggtatttcaa gaaccgctac
      721 gtttcttcct tcgtcggttt cttcccggcc gaccagccgc gggtggtcgg cttcgtagcc
      781 atagatgacc cgtacgggct gcactttggc ggccaggtgg ctgcgccgat cttccagcgg
      841 gtagcccagc gcgtgctgcc gcttttgccc gctccgccgc cgcagctcat tcagccctcc
      901 ctggtcgaaa attcggggtc cgacgcaggg gcctccctct ccgtcaatca gcaaccggcg
      961 agggaagaga acggagagcc gttctttgcg gctcaagcgg cccgggcgga agaggccgct
     1021 ccaaaaataa cgacgccggc gtccctcatc ggccctcccg tgccagagga tctggcccca
     1081 tccgcaaccg ttccggatct ggccggcctg acgatccggg aggcggttgg cgttctcggc
     1141 aggttcgggt tccgggcgcg ggtgcgcggt tccggagtgg tcgtggcgca atcgccggca
     1201 ccggggccgg tacgcggaaa tacgaaaacg gtgatcatca ccggcttgcc gccggtacct
     1261 cgggcatggt gactttggct gagatgataa gcgctcttga ggaaaaagaa gttcgcggtg
     1321 acgttcgggt cgcgatttcg ggtctggagt acgactcgcg gaatataaag agggataatc
     1381 tctttgtggc gttgtccggg ttcgctctcg acgggcatcg ttttgctccc gaggcggtcg
     1441 ctcgcggcgc ttccagtttg gtcgtggagc gctggctgaa tacgcttccc cgtccccaag
     1501 tgcgggtgcc caacagccgc gcggccttag cccgtctcgc acatcagttt tacggccacc
     1561 ccggcgaaaa actcaccgtg atcggtgtca ccggtacgaa cggcaagacc accacagcct
     1621 atctcgtcaa aaaaatatta gaaaccgcgg gggagaaagt tggtctgatc gggacgattc
     1681 aatacgaaat tgccggcgaa gcccttccgg cgtggaatac cacgccggag tctttagacc
     1741 tggcccgctt cttcgtccga cttcaggagg ccggcgcaaa aagctgcgtg atggaggttt
     1801 cctcccacgc cctcgccctc caccgggtgg aggccatcga cttccgggta gcggtcttca
     1861 cgaatttgac tcaagatcac ctcgactacc accggacgat ggaaaactac cgggaggcga
     1921 aagcgaaact cttccgctcc ctgcagggga aagatcgctg ggcggcgatc aatcgcgatg
     1981 accctgaatc agcctacttt gagcaacagt caggcggttc ggtcttcacc tactccctgg
     2041 agggtcgggg ggccgacctc atcgccaccc ggatacaacc cagtgaaaac ggcagccgct
     2101 ttgaactcaa aaccgatgcg ggcacatatg cggtgaattt tcctctggtc gggcgcttca
     2161 atgtggcgaa tgccctggcc gccgcagccg gtgcgcatct cgtcggcatc ggctgggaaa
     2221 cggtggcagt cgggttggag aaaatcgaac ctgtgcccgg gcgggtggag ccggtgcagt
     2281 caggccagcc ctttaccgta gtgatcgact acgcccatac gccggacggc ctgaagcaga
     2341 ttctaacgtt agcccgcagt caatccaaag ccggagtgat tgtggtcttc ggctgcggcg
     2401 gggaccgcga ccgcaccaaa agacctttga tgggggagat cgccggtgcg ttagccgatc
     2461 gggtgatcgt cacttcggat aacccgcggg acgaggatcc gcaaaaaatt attgacgaag
     2521 ttttacccgg cctgcgcggg aagaagaacg tgcgtacctc cgttgaccgg gccgaagcga
     2581 ttgccgacgc gctccacgcc gcccggcgcg gagattgggt cgtcatcgcc gggaaagggc
     2641 atgaagaata tcagatcatc gggaaagaga aaattccctt cagcgaccgg gaagaagtca
     2701 aacgagttct ggggacaatg ggttttcgtg gataagcgaa atgatggaag taatctcttt
     2761 ggctccggta cagacaatgt ttcccatcac cctggatgac gtcatggcgg ccacgggggg
     2821 cagactgctc ctccccgggt tgaatggcac cgccgggatt acgggcgttt ccaccgattc
     2881 gcgcaccctg aaaccgggtg agctgtttgt cgctttatcc ggagaacggt ttgacggaca
     2941 caccttcatc ggcgaggcgg ttggacggca ggcaggtgcg cttttggtcc agcacgacca
     3001 cgaggcgcac ctgcatctgg cttccctctc gctccccgtc ctttccgtac cttccaccct
     3061 ggcggccctg ggcgatctgg gggcttggtt ccggcggaaa tttcccctgc cggtggtggc
     3121 ggtgaccggt tcggtcggca agacgacgac caaagagatg agtgcctgtg tactggaaac
     3181 gaagtaccgc gtccaccggt cggcgggcaa ctataacaat ctggtgggca ttccgctgtc
     3241 cctttttgca ctcaagccgg atcaccaggt gtcggtgctc gaattgggaa tgagcactcc
     3301 cggagaaatt gggcggttga ccgagataac ccgcccggaa gcgggcgtca ttaccaatat
     3361 cgcaccggcg catctggaaa cgatggggtc gctggagaat atcgccaggg caaagttcga
     3421 actgttggac ggcttggggg agaccaaaac cgtcatcctc aatgccgacg accctttctt
     3481 ggcggcgcga gccagacagg agcggcggcc agtggtgacc ttcggcatcc agaaacccgc
     3541 tgatatttgg gccggagatc gcgcacccga tgagaatggt tgctaccgtt tccgggtcaa
     3601 cgatcaaacg gacgtgcgct tggcggtgct cgggttggga aatgtctaca atgccttagc
     3661 gacgttggcc gtcgctacac gcttcgggat cacgtttgag aaggcagcca aggccctcga
     3721 gaactaccgg ccagccaaaa tgcgcctgga ggttttcacc gttgccgggg tgaaaatcat
     3781 cgacgacagt tacaacgcca atccctattc gctgcggtgt gcactggaaa ctttgggcca
     3841 gatgaatccg ccgaaacgag actccggccg ccgggtggcg gtgctggcgg atatgttaga
     3901 gttgggggaa aagcaggaaa cgctgcaccg ggaagtgggc agcgtcgcgg ccaaagcggg
     3961 agtcgatttc ttaatcgccg tggggccttt ggcgggcgag tatctcgccg gagcgactcc
     4021 ggtggggatg ttttcgtcgc agatgagaca tttcgagaat aacccggcgg cgctcgactt
     4081 ccttttgggc tggctcagag cgggcgatct ggtcttggtc aagggttccc gcgggatgaa
     4141 agccgatgca atcgtcaccg gtttgaaaga actcttaccg aaacagtcat aggaggatag
     4201 tgctctatca cctgttctac cccttgagtc aatacatttc cggcttcaac cttttccgct
     4261 acatcacctt ccggaccgcc ggagccacgg tgacggctct tttcatcgcc tgggtgatcg
     4321 ggccgtatat catccggaaa ttgagagccc gccaggtggg ggagaagatt cgcgccgaag
     4381 ggccgcagac ccatctcaag aaggaaggga cgccgaccat gggcgggatc atcattttgg
     4441 cggcggtggt cgttccgacg cttctgtggg ccgacctctc caaccgtttc gtgcaggtgg
     4501 cgcttttggt gacggtctgg atgggggtcg tagggttcgt ggatgactac ctgaaggtga
     4561 tcaagggcaa gcgcaagggg ctggtggcgc gcaacaagtt ttcgggtcaa atccttttag
     4621 ggttgatcgt cggagccatc ctctacttct tccccccggc caaagagttc actccggaca
     4681 ccgacatccc cttctttaaa aactactaca ttcccttcgg cgcgctttac attccctttg
     4741 tcgttttgat catcaccggc acctccaacg ccgtgaactt gagcgacggg ttggacggcc
     4801 tggccattgg cctcgtgggc atttgctttt ttgcttttgc tgggctggcc tacctgaccg
     4861 gccgggtcga tttcagccac tatttgacca tcacctacct cgaaggcgcg ggcgaactga
     4921 ccattttctg cggcgcggta ctgggcgcag ctttgggttt cctctggttc aatgcgcatc
     4981 cggccgaaat tttcatgggt gacaccggcg cgctcgcctt gggggccgcc ttagggacgg
     5041 tcgccgtttt aatcaaaaaa gagattctct tagtaatcgt gggcggggtc ttcgtggccg
     5101 aagcggcctc ggtcattctc caggtggtgt cgttcaagac ccgcggcaaa cggattttca
     5161 aaatggcgcc tctgcaccac cattttgaac tcttgggctg ggccgagccg aaggtggtgg
     5221 tgcgcttctg gattgcggga gcgcttctgg cccttttaac cttgagcacg ctcaagattc
     5281 gctgatgacc gaactcttct cggcagccgc tccggcggcg aaccagagca actttaacgg
     5341 tcagggcaat cacttccggc ccgatcgggt gacggggaag cgggtgggaa ttgtgggcat
     5401 ggcccgctcc ggcctggcct gcgcccggct tttggccaat cagggtgcgg cagtcttttt
     5461 gagcgacacc aagtcggcag gacagttgca gacggaaatc agccaactcc aaaagtgggg
     5521 tctggaatat gaggccgggg ggcatacccg gaaactggtc accgaccgcg attttctggt
     5581 gctttcaccc ggggtgccgc cggacgtgcc gatttgccgg gccgccgaag cccaggggat
     5641 tccggttttc tccgagattg aagtggcctc ctggctctgt ccggcggcga tcgtcggcat
     5701 caccggctcc aacggcaaaa ccaccaccac cagcctggcg ggcgaaatgt tccggaacgc
     5761 ggggtaccat acgctcgtgg cggggaacat cggcacggcc ttttcctcgg tggtggagca
     5821 ggcgactaag gcgagcgtcg tggttctgga aatttccagt ttccagttgg aaaagatcga
     5881 ggagttccgc cccagaacgg cagtgttgct caatctctcg cccgaccacc tcgaccggta
     5941 cgggaattac gaggcataca aacaggccaa actgcggatt tttgaaaacc aaaaggagac
     6001 tgactttgcg gtgctcaatg ccgacgactt agggctttcc gatctgccgc ccaaattggc
     6061 ggcgaaagtg gcattctttt ccaccaaagg agaagtcggt tttggcgcct gtatccgcca
     6121 gggcgagatc tgcctcaagc gcaacggcca ctacgacacg gtgctgccag cagcgaaggt
     6181 cggcatcccg gggccgcata acctgtcgaa tgcgaccgcc gcctgcgcca tcggcctttc
     6241 cttcggcctc gcgccggagg tcatggcccg ggccttggcc gagttcaaag gggtcgagca
     6301 ccggttggag ttcgtggcgg aaatcgacgg ggtaaaattc atcaacgact ccaaagccac
     6361 gaacgtcgat gcggtctggt acgcgctcca gagcgtcccg cagccgatcg tcctcatcgc
     6421 cgggggcaaa gacaaaggcg ggagttacgc gccgttgcgc gagttcgtgg cccaacgggt
     6481 gaaaaagttg attttaatcg gccaggccaa agagaaaatc aaggaagcac tgggcgacgt
     6541 ggtgacgacc gtcactgccg aaagcctccc ggaggcggtg gaggtcggtt tttccagcgc
     6601 ggcacccgga gacacggtgc tcttgtctcc ggcctgcgcc agtttcgata tgttcaccaa
     6661 tttcgaagag cggggacgga ttttcaaaca aacggtggcc ggactcaagg ggcgtacggg
     6721 cggaggcgac caatgagccg ccggattgat tttttcctcc tcctgataat cctctctctt
     6781 ctggccatcg gcacggtgac ggtctatagt tcttccgcct accttggaga ggtgcgctac
     6841 cggaacccgg cggtctactt ccaccggcag gtgatctggg ccgggctggc cctggtgacg
     6901 ctctggtttt tctcccgcat tgactaccac cgctggagac ctctggcccc ctgggggctg
     6961 gccggaggca ttctcctgtt aatagcggtc ctgttttttc ccgccaaaaa cgacgcgcac
     7021 cgctggatcg acttcgggcc gattcagttc cagccgtcgg agctcttcaa attagcggtc
     7081 attctgtatg tcgcggccgc gctggccaag cgggggcaca aaatttctga cctgcgctac
     7141 cttttgctgc cgtacgcgcc gcttttggga gtggcgttca ttttgctcat gctggagccg
     7201 gatttgggaa cttttctcgt catcgcgggc atcgtctttt catctttggc catcgccggg
     7261 gcacggtgga agcacttact caccggattt ttggcagcgg ctctggtggt ttcggtactc
     7321 gtcgtcggat tgggctacaa gcagcagcgg gtcaaagact acgtcaaatc ctgggaagat
     7381 cccctggccg ggagctacca ggtgaagcag gcgatgttgg gtttgagttt gggtggattg
     7441 accggggccg ggttgggcga aggacgccag aagatgctct atctccccgc gccgcacacc
     7501 gacttcattt tcgccgcaat cggagaagag ggaggacttc tctggctggc gctcatctta
     7561 gcgctgttct tcgttttaat ctggcgcggg tggctcgtcg cccagcaggc cccggatcgt
     7621 tttgggtttt ccctggcgtt cggcattacg gctttacttt tcaccagcat caccttaaac
     7681 gtcggcgtcg ttttaggcct tttacccacc acgggcattc cgctgccctt tttgagttac
     7741 ggcggcacgg cgctattggc tgcctccgcc gggtgtggtg tgctcttaaa tattgcgagt
     7801 cagaaggaga tgcaggcgcg gtatggctac gtacggtaag ttgaaaattc tttttgccgg
     7861 cggcggcacc ggcgggcatc tctttccggc gattgctctg gcgcaggcag cagagaaagc
     7921 cttccccggc gtccaggtcg ttttcgtcgg cgcaccgcag gggctggaag ccaaaaaaat
     7981 tcccgagttg ggctggcgct tgcacctcgt gccgattcgc ggtctctcac gcacacgtcc
     8041 gttctcgaat ttggttttcc cgttctccgt agtgcggtcg ctggtacagg cgaatcgcgt
     8101 cctttcgctg gagcaacccg acctcgtggt gggcagcggc ggctatgtgg cctggccggt
     8161 tttgcgcgcc gcggttgcca agagtattcc gactttgatt caagagcaga atagctaccc
     8221 gggtatcgtg acccggcgc
//
LOCUS       DBNGZD010000139         3672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_648226, whole genome shotgun sequence.
ACCESSION   DBNGZD010000139 DBNGZD010000000
VERSION     DBNGZD010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_648226"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC16GM_03800"
     CDS             complement(<1..310)
                     /locus_tag="AC16GM_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922833.1"
                     /translation="MAQYDCTLAMNTTATAAAGKIAPANERTLRRDTWWLQPLLTVIG
                     LGGFAIYATWVAFQNANYLVEPYLSPFYSPLIKVSWWSFSPAFLVLWAPLGFRATCYY
                     Y"
     gene            complement(383..571)
                     /locus_tag="AC16GM_03805"
     CDS             complement(383..571)
                     /locus_tag="AC16GM_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922834.1"
                     /translation="MAKKQSFADKASKVKHTVTCPVCHSAQTPTLYIKSEQSPGGAWK
                     FRQVHVNVCKCNQAEVMG"
     gene            complement(683..2692)
                     /gene="uvrB"
                     /locus_tag="AC16GM_03810"
     CDS             complement(683..2692)
                     /gene="uvrB"
                     /locus_tag="AC16GM_03810"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR2922835.1"
                     /translation="MAQFELVSDFPPSGDQPKAIDQLAEGLQNGANHQVLLGVTGSGK
                     TFTIANVIARLGRPTLVISHNKTLAAQLYGELKGFFPRNAVEFFISYYDYYQPEAYVP
                     QTDTYIEKDTSINDDIDRLRLRATSSLLEREDVVIVASVSCIYGIGSPAEWKEQMLLL
                     EVGETVERDHLLRRLIDIHYNRNDFEFSRGTFRVRGDVVEVIPAYEEHALRIELFGDQ
                     IERISVIDPLRGEIAAEKKKAAIYPAKHFVTTQPRLEAAIKNIEAELHERLAHFRSQG
                     KLLEAQRLESRTKFDIEMMKEIGYCTGIENYSRHLSGRKPGERPFCLLDFFPKDFLLV
                     IDESHQTVPQIQGMYHGDRSRKEVLVEYGFRLPSALDNRPLTFDEFEALADRVIYDSA
                     TPAEYEIKKTRGVVVEQIIRPTGLIDPKITVKPLDKQVDDLLEEIRARAASHERVLVT
                     TLTKRMAEDLADYLAQMGIKVRYLHSEIEAIDRVEILRNLRLAEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSERSLIQIAGRAARNKAGEVIMYADAVTDSMRKAIDET
                     DRRRKLQEEYNRTHGIEPQTIYKTKEEILRATAFADSRTAAVPGEPVEKLGFLEKMEL
                     EDQLAYLTKEMKKASGTLDFERAAAFRDKIAQLKESLKKKTKPVR"
     gene            2867..3379
                     /locus_tag="AC16GM_03815"
     CDS             2867..3379
                     /locus_tag="AC16GM_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR2922836.1"
                     /translation="MKFSLHAKDDPHRLLHLEAAPDLLHIAYGEVRFARPVVCDVSVD
                     KRGDAFVCHGEIRTVVGLDCDRCATPFDHRVGAAIDLVIQRVTGGPAKDELDADYVEL
                     PPHVSEYDLSDRVRDAVILALPIHPLCQAGCRGLCPTCGRNLNSGSCNCRPESSDSRW
                     EDLKKLKYSL"
     gene            3389..3571
                     /gene="rpmF"
                     /locus_tag="AC16GM_03820"
     CDS             3389..3571
                     /gene="rpmF"
                     /locus_tag="AC16GM_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR2922837.1"
                     /translation="MPLPKRRHSKTRGAKRRTHWKLEPPAVVECSHCHQPKVAHRVCP
                     HCGYYDGKEVIAPEEA"
     gene            3578..>3672
                     /locus_tag="AC16GM_03825"
     CDS             3578..>3672
                     /locus_tag="AC16GM_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922838.1"
                     /translation="MSAPVKIAVDAMGSDHGPEAVVAGAVLALRE"
BASE COUNT          760 a         1014 c         1094 g          804 t
ORIGIN      
        1 ggtagtagta gcaggtggcc cggaaaccga gcggcgccca gaggacgagg aatgcgggcg
       61 aaaaagacca ccaggaaact ttgattaagg gcgagtagaa gggcgaaagg tacggctcca
      121 ccagataatt cgcgttctgg aaagccaccc aggttgcata gatagcgaat ccgcctaatc
      181 cgatgacggt caaaagcggc tgaagccacc aggtgtcccg gcggagggtt ctctcatttg
      241 cgggagcgat ttttccggct gctgcggttg cggtcgtatt catcgccaag gtgcaatcgt
      301 attgtgccaa ataaagtttg cgccaaggaa ttgtcaataa aaaaagcggc cttacggccg
      361 ctttttcaac ccaatcactc ttttatccca tcacctcggc ctggttgcac ttgcagacgt
      421 tgacgtgcac ctggcggaac ttccaggcgc cgccggggct ctgctccgac ttaatgtaga
      481 gggtcggggt ctgcgccgag tggcaaaccg ggcaggtgac ggtgtgcttg accttggatg
      541 ctttgtccgc gaacgactgt ttcttggcca tttaagcctc cgttcttcta ctcaagtata
      601 cgtagaaaca tttgggttgt caaagagccg gacaacgatt tttgaccgga gaagagaaac
      661 ctcccgtccg agagaacgcg gattatctca ccggcttcgt ttttttcttc agtgattctt
      721 tcaactgcgc aattttgtcc cggaaggcgg cggcgcgttc gaaatccagg gtcccggatg
      781 ccttcttcat ctctttggtc aagtaggcga gttggtcttc caattccatt ttttccagga
      841 aaccaagttt ctccaccggt tcgcccggaa cagcggcggt gcgggagtcg gcaaaggcgg
      901 tggcgcggag gatttcttct ttggtcttat aaatcgtctg cggctcgatg ccgtgcgtcc
      961 ggttgtactc ttcttggagt ttccgccgcc ggtcggtttc gtcgatggct ttgcgcatcg
     1021 agtcggtgac cgcatcggcg tacataatca cttccccggc cttattgcgg gcggcgcgac
     1081 cggcaatttg aatcagcgag cgctcggagc gcagaaaacc ttccttgtcg gcatccagaa
     1141 tggcgacgag agaaacctcc ggcaaatcca gtccttcccg caggaggttg atccccacca
     1201 ggacgtcgaa ctcggccaag cgcaggttgc gcaggatttc gacccggtcg atggcctcaa
     1261 tctcagaatg caggtagcgg accttgatcc ccatctgcgc caggtaatcg gccaagtctt
     1321 cggccatcct tttcgtcaag gtcgtcacca ggacccgctc gtggctggcg gcccgggcgc
     1381 ggatttcttc cagaaggtca tccacctgct tgtcgagcgg tttgacggtg atttttggat
     1441 cgatcaagcc agtcgggcgg atgatctgct cgaccaccac cccccgtgtc tttttgattt
     1501 cgtactcggc cggcgtggcg gaatcgtaaa ttacccggtc tgcaagcgct tcgaactcgt
     1561 caaaggtcaa gggccggtta tccagagccg aggggaggcg aaagccgtac tcaaccagaa
     1621 cttctttccg cgaccggtcg ccgtggtaca tcccctgaat ctgggggacg gtctggtgcg
     1681 actcatcgat gacgagcaaa aaatctttgg ggaaaaagtc aagcaggcag aagggacgct
     1741 ctccgggttt ccgtcccgag aggtgccgcg aatagttctc aatccccgtg cagtagccga
     1801 tttccttcat catttcaata tcgaacttgg tgcgggactc caggcgctgg gcttccaaaa
     1861 gtttcccctg agaacggaag tgagccaggc gctcatgcaa ttcggcctcg atgtttttga
     1921 tcgctgcttc gaggcgcggc tgggtggtga cgaaatgctt ggccgggtag atggcggcct
     1981 tctttttttc ggcggcaatt tctccgcgca ggggatcaat caccgaaatc cgttcaatct
     2041 gatcgccgaa caattcaatg cgcaacgcgt gctcttcgta agccgggatc acctccacca
     2101 cgtcgccccg gacgcggaac gtcccgcggg agaattcgaa atcatttcgg ttgtaatgga
     2161 tgtcgatcaa ccggcggagg aggtggtcgc gttccacggt ttcgcccact tccaggagca
     2221 gcatctgctc tttccactct gcgggcgagc cgatgccgta aatgcaggag accgaggcga
     2281 cgattaccac gtcctcgcgc tccagaagcg aactcgtggc gcgcaggcgc agccggtcga
     2341 tgtcgtcgtt gatcgaggtg tctttctcga tgtaggtgtc ggtctgaggc acgtaggcct
     2401 ccggctggta gtagtcgtag taggagatga aaaactccac ggcgttacgg gggaagaacc
     2461 ctttcagttc gccatagagc tgtgcagcta aggtcttgtt gtgggaaatg acgagggtgg
     2521 gccggcccag ccgggcaatc acattggcaa tcgtgaaggt tttcccggag cccgtgactc
     2581 ccaaaagcac ctggtgattg gcgccgtttt gcagtccttc ggctaattgg tcaatcgcct
     2641 tgggctggtc gcctgagggc gggaaatcag aaacgagttc aaactgagcc ataaggtaag
     2701 tgataagaat cgggcataac agtcgcctgt taataaatat acgtacaaat attcttagaa
     2761 caaagagatg aaggtcgcaa gttaagagag ttgatagagt taccaacccg gactgcgccg
     2821 ggaaggagct tgacaaccgc actgctgcaa cgtatcctgt ttgcccatga aattcagcct
     2881 tcacgcgaaa gatgatccgc accgcctgtt gcaccttgaa gccgcacccg acctgctcca
     2941 catcgcctac ggcgaggtgc gctttgcccg gccggtggtc tgcgacgtga gcgtggataa
     3001 gcgcggggac gctttcgtct gtcacggcga gattcggacg gtcgttgggt tagattgcga
     3061 tcggtgtgcg accccttttg accaccgggt gggggcggcc atcgacttag tcatccagcg
     3121 ggtgacgggc ggtccggcca aagatgagtt agatgccgat tacgtggaac tgccaccgca
     3181 cgtcagtgag tacgatctct cggaccgggt acgggatgcg gtcattctcg ccttgccgat
     3241 tcaccccctg tgccaggccg ggtgccgggg gctttgcccg acctgcggcc gcaatttgaa
     3301 ttccggttcc tgcaactgcc gaccggaaag ctccgactcg cgctgggaag acttaaagaa
     3361 actgaaatat tcactctgag gaaccagtat gcccttaccc aaacgccgcc actcgaagac
     3421 gcggggcgcc aaacggcgca cacactggaa attggagccg ccagccgtgg tggagtgttc
     3481 gcactgtcac cagccgaagg tcgcgcaccg ggtctgcccc cactgcggct actacgacgg
     3541 caaagaagtc atcgcgcccg aagaggcgta gcccggaatg tccgcaccag tcaagattgc
     3601 ggtggatgcc atgggttccg accacggccc ggaggcggtg gtggccggag cggttttggc
     3661 cttgcgcgaa aa
//
LOCUS       DBNGZD010000140         8797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_93579, whole genome shotgun sequence.
ACCESSION   DBNGZD010000140 DBNGZD010000000
VERSION     DBNGZD010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_93579"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..895)
                     /locus_tag="AC16GM_03830"
     CDS             complement(50..895)
                     /locus_tag="AC16GM_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2922839.1"
                     /translation="MRKFSVMLVAGIVLVGDAAWAGPFVDSVIVASKAFALGSQGIIE
                     VYIHNDSALNGIALPIVIKSQSGGAFATVNRGGGVDSIQMVVAHPGAPIYNLTRINGT
                     DPDSLFILWIDLSGGTIAAGTRREAFRIFVTNNSNAGVYVLDSNRLNASQTLKFTHPP
                     TQSSVPKYILGRDTVGLTAVREEHNSSLPTSFALSQNRPNPFNPTTTITFALPKESEV
                     TLEVFNLLGQKVIDLVDEHLSAGTKSVEWDGRDALGREVPSGIYFYRMRAESFAETKK
                     MVLMK"
     gene            1484..3025
                     /locus_tag="AC16GM_03835"
     CDS             1484..3025
                     /locus_tag="AC16GM_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2922840.1"
                     /translation="MRRAFILLLGALAFFFTTLIALAPPCNLYTKNGAGAGDWFGYRV
                     SGTGDVNGDGKADFIIGAPYTNPGGLTDAGSAYVYSGANGALLYQKNAESSGDAFGST
                     VAGAGDVNGDGKADFVVGAPYSDPGLVPLGSAYVYSGSDGALLYQKNGTDYEVTLFGY
                     SVNGAGDVNGDGKGDFIISAPLTDSISSMGELVFEDCGSAYVYSGSNGALLYKKNGEA
                     NNDHFGRYSVAGAGDVNGDGKADFMGASILGESNRGYAIVFSGDNGISLYRKTGAAAG
                     DEFGYSVDGAGDLNGDGKADFIIGAPKTDPGGRTNAGSAYVYSGADGNLLYQNDGASA
                     GDELGWSVAGTGDMNLDEVPEFIVGAYEADPGGRTDAGSAYVYSGGNGSLIDQRDGIS
                     AGDKFGNSVDGAGNVNGTGGDEFIVGSPYTDTTGKVDRGSAYVYRYCGLLSSPPCVAR
                     GDVTNDGLIDIADIVALIQDVVFETGVPNGLAGDADCDAVRDISDIILLIQYVVFGTP
                     SPCCL"
     gene            complement(3132..8492)
                     /locus_tag="AC16GM_03840"
     CDS             complement(3132..8492)
                     /locus_tag="AC16GM_03840"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2922841.1"
                     /translation="MTITVANSNLAPVLDSIRAKTVAENQLLQFRVTSSDPDLTTPSL
                     SAINLPVGAVFVDSGNSRGSFTWTPTFFQAGTYNVTFIASDGSLADSELVAITVTNVN
                     RAPVLDSVRARSVAENSLLQFLVTSSDPDSTVPALQAVNLPVGAVFVDSLNGRGSFTW
                     TPSFFQAGIYNVRFIASDGSLADSELVTITVTNVNRAPVLDSIRSKTVAENSLLQFRV
                     TSSDPDLTNPTLNTVNLPTGAVFVDSLNGRGSFSWTPSFFQAGTYNVTFIASDGALAD
                     SEGVTITVTNSNLAPTLDSIRAKSVAENQLLQFRVTSSDPDLTTPSLSAINLPVGAVF
                     VDSGNSRGSFTWTPTFFQAGTYNVTFIASDGSLADSELVAITVTNVNRSPVLDSIRAK
                     TVPENSLLQFRVTSSDLDSTIPSLSAVNLPVGAVFVDSLNGRGSFTWAPSFFQAGIYN
                     VRFIASDGSLADSELVTITVTNVNRAPVLDSIRAKTVAENQFLQFRVTSSDPDSTTPS
                     LNAVNLPVGATFVDSLNGRGSFSWTPSFTQAGTYGVTFIASDGSLADSESVTITVTNS
                     DRAPVLDSIRARAVAENQLLQFRVTSSDPDLQTPSLVAVSLPAGAVFVDSGNSRGSFT
                     WTPTFDQSGLYTVTFIASDGTLADSELVSLTVTNVNRPPVLALIGARNGAEGQRLVFV
                     ISASDPDPQTPTLAGFSLPAGAVVFDSSNGRGTFDWTPNFSQAGTYQVLFVASDGALA
                     DSETVTITIGNSNQAPILAPIGARTTSQGQILTFSISAVDSDLTVPVLSAENVPLNAT
                     FVDSGNGRGSFRFAPSFTQSGVFTVRFIASDGSLADSELVPITVNFVNKPPILAAIGA
                     KTVAEGSLLSFRVTATDSNGTTPRLFAQNLPVNATFTDSLNGAGGFVFQPSFVQSGIY
                     QVLFFASDSLAADSELVQITVTDAGNQAPRIDSLGPFTVRENDSLVFVVRAVDPESGA
                     VRLAMVKGPTGAVFTDSLNGHGLFRFRPTYLQASIDSAVFTATDNAVPPLSASRRVQI
                     TIVDANQPPVLDSIGPKAVLAGQVIQFRVHAKDTTDASPGRLFLSASPLPLHATFVDS
                     ANGAGSFRFAPDFTQIGFDTVSFLVFDDDLPVMSDREDVVIEVRSANRAPVLDPIGTK
                     MVREGDVLSFRVHATDPDGTIPRLYTSALPQNATFVDSGNGAGSFRFTPNFVQAGLYS
                     VTFSATDNIATAREAVLIQVTEAGNQRPILTTPDTTTLTEAETVVLPVFASDPDTTIP
                     RLSAFNLPPNATFADTGRGSGVFRFTPSYIQAGTYTIGFVASDGALADTNLMTVIVRD
                     AGNQPPVLDSIPDRTVTEGGTLTFTVHASDPDTTKPFLTAAPLPANATLVDNRNFTAT
                     FSFSPSFFQAGQYQITFYARDFLTTALVDSQKVTITVTNVNQFPIVDSIVSRPSSRTI
                     AEGETLLAVVYARDPDNTIPRLTAANLPLQSNFVDSGNGHGLFRYTPDFCQTGNSAQK
                     FFSVVFTATDRDADSATATQSLQITLNNTQKAPVLNPIGPLSITEGDTLRTVISSFDP
                     DCTTPTLAVQNLPRNATFTSSSGVGLFTFAPGFNQAGTYNVLFIATDNTGRADSELVA
                     VTAVEFGNHAPQITLTAPLPTPLTRGDTTILRVGDLSSARVSATDADSTIPVLSAVPV
                     PRNAVFVDSGNGRGVFRITPDSSQIDSTYLVRLIASDGLATDTVLLKFRIRPFFRGDC
                     NHDGVIDLSDIVQLIQYVVNDQFPPIPPWAGDVKLDGVIDLVDVVGLISFVVNGSPVP
                     LP"
     gene            complement(8541..>8797)
                     /locus_tag="AC16GM_03845"
     CDS             complement(8541..>8797)
                     /locus_tag="AC16GM_03845"
                     /inference="COORDINATES: protein motif:HMM:NF036814.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922842.1"
                     /translation="GSLADSELVTITVTNVNRAPVLDSIRAKSVAENSLLQFRVTSSD
                     PDSTLPALSAVGLPAGAVFVDSLNGRGSFSWTPSFFQAGT"
BASE COUNT         2016 a         2383 c         2571 g         1827 t
ORIGIN      
        1 atcaataaaa aagccccgct gggcggggct ttttaaattc atttccggac tacttcatca
       61 gcaccatctt cttggtttcg gcaaaactct ccgcccgcat ccggtagaag taaatcccgc
      121 tgggcacttc ccggcccaga gcgtcgcgcc catcccattc caccgacttg gttccagcgg
      181 acaggtgctc atcaaccaaa tcaatcactt tctgtcccag gagattgaac acctccagcg
      241 ttacctccga ctctttgggg agcgcaaagg taatggtggt ggtggggtta aacggattgg
      301 ggcgattctg cgaaagagca aacgaggtcg gcagactcga gttgtgttct tcgcgcactg
      361 ccgtgagacc gaccgtgtcc cgacccagaa tgtattttgg aacactactc tgagttggtg
      421 ggtgagtgaa ttttagtgtt tggctggcat tcagtcggtt cgaatctaag acgtaaactc
      481 cagcattgct gttattggtc acaaatatcc ggaatgcctc acgccgtgta cccgctgcaa
      541 tggtaccccc actcaaatcg atccacaaaa tgaacaaaga gtccggatcg gtaccattta
      601 tcctggttaa attgtaaatt ggcgctccgg ggtgcgctac caccatttga attgaatcca
      661 ccccaccacc tctgttgacg gtagcgaatg caccccccga ttgtgacttg ataacaatgg
      721 gcagggcgat tccgttgaga gcggaatcat tgtggatata cacttcaatg atcccttgcg
      781 acccgagggc aaatgcttta gaagcgacaa tgaccgaatc aacaaaaggt ccggcccacg
      841 ccgcatcgcc aacaagcaca atacccgcca ccagcatcac cgagaatttt cgcatccgat
      901 tactcctctc ttcagtccct cagattccgg tagagggttt cacgcactcc cggaaagtca
      961 atttccgctt agaatactac ttccgaaaaa caaacgcaag tccttttcgt aaaaaaatca
     1021 atctcgctca accgcccatc ccacctcctt gttcacacaa atatcaattc cttttcactt
     1081 gcgccaggtg gactgctcgc aagaggcaat tgtcctaaat tatccccccc gccaattcct
     1141 gtcaatagaa aaattggggg tggcaaaaaa aagttggact tagagccgag cgtaaaggac
     1201 ggtggaaatc ttcaaagaaa aacctcgctg gcggaaatag ggctgaaggg tagtcctttg
     1261 cgtgcttcta acttcgcggt tctcgagaac aaagtgggag ataatgagaa attaagagaa
     1321 gccccctaat ttcaagggga aatatgatca acaagtaacc taagtagttt gtagataaga
     1381 ggttctattt ttttcttcac agaaaagaat tctcttgaca cctgcctgag atttagcttt
     1441 gttacgagca gaaagaaagt taagtcaatg ttagggagga cctatgcgaa gagcatttat
     1501 acttttgctg ggtgctttgg ctttcttctt caccacatta atcgcacttg ccccaccctg
     1561 taatctttac accaaaaacg gtgctggcgc aggtgactgg tttggctatc gagtatctgg
     1621 aaccggcgat gtgaatggcg atggaaaagc tgattttatc atcggtgctc catacactaa
     1681 tccaggggga cttactgacg ccggttcggc gtacgtctat tctggagcta atggggcatt
     1741 gctttaccaa aaaaacgccg aatccagtgg tgacgcattc gggagtacag tggcaggggc
     1801 tggagatgtt aacggcgatg gcaaggctga ttttgtcgtg ggcgctcctt actccgaccc
     1861 cgggttggtc cctttaggtt ctgcttacgt ttactctggt agcgatggtg ccttgctgta
     1921 ccaaaagaac gggacggact atgaagttac gctttttggc tattctgtga acggcgcagg
     1981 ggacgtgaat ggagatggga agggcgattt tataattagc gcccccctta cagattctat
     2041 cagctcaatg ggcgagttgg tgtttgaaga ctgcggttcg gcttatgtct attcgggttc
     2101 caacggcgcc ctgctttaca agaaaaacgg tgaggccaat aatgatcact ttggtcggta
     2161 cagtgtagcc ggtgctgggg acgtgaatgg cgacgggaaa gctgacttta tgggtgcctc
     2221 tatactgggc gagtccaatc gaggctacgc cattgtcttt tccggtgaca atggcatatc
     2281 actttacaga aaaactggcg ccgccgccgg tgatgaattc ggctattcgg ttgacggtgc
     2341 cggagatttg aacggtgatg gaaaagccga ctttataatc ggtgctccca agaccgaccc
     2401 aggcgggcgg accaacgctg gctccgcata cgtttattcc ggagctgacg gcaatcttct
     2461 ctatcaaaac gatggcgcat ccgccggtga tgagctcggg tggtcagtgg ccggtaccgg
     2521 agatatgaat ctcgatgaag ttcccgagtt tattgtcggc gcctacgaag ccgacccagg
     2581 tggtcgtact gatgctgggt cagcttatgt ctattctggc ggcaatggct cattgattga
     2641 tcagagagat ggtatcagcg ctggtgacaa gtttggcaat tcagttgatg gagcggggaa
     2701 cgtcaacggc actgggggag acgaatttat tgtcggttct ccttatacag atacgaccgg
     2761 gaaagttgat cgtggctcgg cttatgttta ccgatattgt ggtctgctat cttctcctcc
     2821 ctgcgtggcg cgcggagatg taaccaacga tggcttaatc gatattgcgg acatcgtcgc
     2881 cctaatccag gatgtagtct ttgaaaccgg cgttcctaat gggctggcgg gcgacgcaga
     2941 ttgtgacgcc gtccgagaca tatcggacat tatcctgcta atccaatacg tggtattcgg
     3001 aacgccaagc ccgtgctgcc tctaaaggcc tgcttttaga gtctgattta aaaattgccg
     3061 aagatttttc cgaaaatctc cacaccctcc aactaagtag gggggtcttc tcatatctga
     3121 gaaaagtcga tctacggtag cggcacgggg ctgccgttaa ccacaaagga gattaaaccg
     3181 acaacgtcca ccaggtcgat gacgccgtca agtttgacat cccccgccca cggaggaatg
     3241 ggtgggaatt gatcattgac cacatattgg atgagttgga caatgtcgga caggtcaata
     3301 accccatcgt gattacagtc gccgcggaag aaaggccgga tgcggaactt caggagcacc
     3361 gtatcggttg ccagtccgtc cgaagcgatg agacgaacga ggtaggtcga atcgatctgg
     3421 ctggaatcgg gagtaatccg aaatacaccc cgtccattcc cggaatcgac gaagaccgcg
     3481 ttccgcggca cgggcaccgc ggaaaggacc ggaatggtgc tgtcggcgtc agtggcggaa
     3541 acacgcgccg atgagagatc gccgacccgc aggatggtcg tatctccccg cgtgagtggg
     3601 gtcgggaggg gcgccgtcag ggtaatctgc ggcgcatggt tcccgaattc aacggccgtc
     3661 acggccacca gttcgctgtc ggcccggccg gtattgtccg tggcgatgaa gagcacgttg
     3721 tacgttcccg cctggttgaa acccggagcg aaggtgaaaa gaccgacccc gctgctcgac
     3781 gtaaacgttg cgttgcgcgg caagttctgc accgccagcg tgggggtggt acagtcgggg
     3841 tcaaaagacg agatgacggt gcgcaacgtg tccccctcgg tgatgctcaa aggcccgatc
     3901 ggattgagta ccggcgcctt ctgggtgttg ttcagggtga tctggagcga ctgggtggcg
     3961 gtggcggaat cggcatcccg gtcggtggcg gtgaagacga ctgagaagaa cttttgcgca
     4021 ctgttgccgg tctggcagaa atccggagtg tagcggaaga ggccgtgccc gttgccggaa
     4081 tcgacgaaat tagattggag cggcaggttc gccgcggtca ggcgcggtat ggtgttgtcc
     4141 gggtcgcgtg cgtagaccac cgccagaagc gtttctccct cggcaatggt gcggctggac
     4201 ggccggctga cgatcgaatc gacgatgggg aactgattca cgttggtcac cgtgattgtc
     4261 accttctggc tgtcgactaa ggccgtcgtc aaaaagtctc gggcgtagaa ggtaatctgg
     4321 tactgccccg cctggaagaa actcgggctg aaggagaaag ttgcggtgaa gttccggtta
     4381 tcaaccaaag tggcgttggc cggcaaaggc gccgccgtca ggaatggttt ggtcgtatcc
     4441 ggatcgctgg cgtgcacggt aaaggtgagc gtcccgcctt cggtgacggt tctatcggga
     4501 attgaatcca gaaccggtgg ctggttgccg gcatcccgga cgatcaccgt catcaagttc
     4561 gtatccgcca gcgcgccatc cgacgccaca aaaccgatgg tgtacgtccc cgcctggatg
     4621 taggaagggg tgaatcggaa gaccccgctg cccctgccgg tgtccgcaaa cgtggcgttc
     4681 ggaggcaggt tgaaagccga aagccgcgga atggtggtat cgggatcgct ggcgaagacc
     4741 ggcagaacga cggtctcggc ctcggtgagc gtggtggtat cgggggtggt caggatgggc
     4801 cgctggttgc cggcttcggt cacctggatc aagactgctt cgcgcgccgt ggcaatatta
     4861 tccgtggctg agaaggtgac tgagtagagc ccggcctgga cgaaattggg agtgaagcgg
     4921 aacgagccgg caccattgcc gctatcgacg aacgtggcat tctgcggcag tgccgacgtg
     4981 tacagacgcg gaatcgtccc gtccgggtcc gtggcatgca cgcggaatga taggacatct
     5041 ccctcgcgca ccatcttcgt tccgatcgga tcgaggaccg gcgcccggtt ggccgagcgc
     5101 acttcaatca ccacatcttc ccggtcactc atcaccggca ggtcgtcatc gaagaccaag
     5161 aaggaaacgg tgtcgaaacc gatttgggta aagtcgggcg caaaccggaa cgatccggcg
     5221 ccattggccg agtcgacaaa ggtggcgtgg agcggcagcg gggaagcaga aaggaacaga
     5281 cggcccggac tggcatcggt cgtgtctttg gcatgcaccc ggaactggat tacctgcccg
     5341 gctaagacgg ctttgggacc aatcgaatcc aaaaccggtg gctgattggc atccactatg
     5401 gtaatctgca cccggcgcga ggccgaaagc ggcggcaccg cgttatcagt ggcggtgaag
     5461 accgccgagt caatgcttgc ctgcaggtag gtcggccgga agcggaagag gccatggccg
     5521 ttcagactgt cggtgaagac cgcacccgtc gggcctttga ccatcgccag gcgcacggca
     5581 ccgctctccg gatcaacagc ccgcacgaca aaaaccagac tgtcattttc acggacggta
     5641 aaaggaccga gcgagtcgat ccgcggcgcc tggttgccgg catccgtaac cgtgatttga
     5701 acgagttcgc tgtcggccgc cagagaatcc gatgcgaaga agagaacctg gtagatacct
     5761 gactgcacga aactcggttg gaaaacaaac ccgcccgctc cgttcaaact atcggtgaat
     5821 gtagcgttca ccggcaggtt ctgggcgaag agtctgggcg tggtgccgtt ggaatcggtg
     5881 gccgtcaccc ggaaggagag gagcgaacct tcggctacag ttttggcacc gatggcggcc
     5941 agaatcggcg gcttgttcac gaaattgacc gtgatcggga ccagttcgct gtccgccagg
     6001 gaaccatccg acgcgatgaa ccgcaccgta aaaacgcccg actgggtgaa cgacggcgca
     6061 aagcggaagg agccgcgacc gttaccgctg tcaacaaaag tggcgttgag tggcacgttt
     6121 tcagccgata agacgggcac ggtcaagtcg gaatctacgg cagatatact gaaagtgaga
     6181 atttgcccct ggctggttgt cctggcgcca atgggagcta agatcggcgc ctggttggag
     6241 ttgccaatgg tgatggtgac cgtttcgctg tcggctaaag cgccgtcgga agcgacaaaa
     6301 agcacctggt aagttccggc ctggctgaaa ttcggcgtcc aatcgaaagt cccacgccca
     6361 ttgctgctgt cgaataccac cgcaccggca ggcaggctga acccggccag ggttggcgtt
     6421 tgcggatcgg ggtcgctcgc cgaaataacg aagaccagcc gctggccttc cgccccgttt
     6481 ctggctccta tcagtgccaa aaccggcggc cgattgacgt tcgtgaccgt gagcgaaacg
     6541 agttcgctat ccgccagcgt gccgtcggag gcgatgaagg tgacggtata gagaccggat
     6601 tggtcaaaag tcggcgtcca ggtgaaactc ccgcggctgt tgccgctgtc cacaaatacg
     6661 gcacccgccg gcagactgac cgccacaagc gagggcgtct gcaggtccgg gtcgctcgag
     6721 gtcacccgga attgcaggag ctgattctcg gctaccgccc tggcgcgaat tgagtctaag
     6781 accggcgccc ggtcggaatt ggtcacggtt atggttacgg actcgctgtc tgccagtgag
     6841 ccgtccgagg caatgaaggt gacgccgtac gtccccgcct gcgtgaacga gggcgtccag
     6901 ctgaaactcc cgcgaccgtt cagcgaatca acgaaagtgg cacccaccgg gagattaacc
     6961 gcattcaacg acggtgtcgt cgaatccgga tcgctggagg tcacgcggaa ctgcaagaac
     7021 tggttctcgg caacagtttt tgctcggatg gagtcaagca ccggcgcccg gttgacgttc
     7081 gtgacggtga tggtgaccaa ttcgctgtcg gctaaagaac catccgacgc aatgaaccgc
     7141 acgttgtaaa tccccgcctg gaagaaagag ggcgcccagg tgaatgagcc gcgcccgttc
     7201 agtgagtcca caaacaccgc tcccactggc aagttcaccg cgctcaaact cggtatcgtc
     7261 gaatcaagat ccgatgaggt cacccggaac tgcaaaagac tgttctccgg caccgtcttc
     7321 gctcggatag agtcgaggac tggtgaccga ttcacgttag taaccgtgat cgccaccagc
     7381 tcactgtcag ctaaggaacc atccgacgca atgaaggtga cgttgtacgt ccccgcctgg
     7441 aaaaaggtcg gcgtccaggt gaaactcccg cggctgttgc cgctgtccac aaagaccgca
     7501 cccaccggca aattgatcgc gctcaaagaa ggcgtggtca aatcgggatc gctggaggtc
     7561 acccggaact gcaggagctg attctcggct acgctcttgg cccggattga gtccagagtt
     7621 ggtgccaagt tgctattcgt cactgtaatc gtcacccctt cgctgtcggc cagggcgcca
     7681 tccgaagcaa tgaacgtgac gttataggtt cccgcctgga agaaggaggg cgtccagctg
     7741 aaactcccgc gcccgttcag tgaatccaca aataccgcac ccgtcgggag attgacggta
     7801 ttcaaagtgg gattggttaa atcaggatcc gaggacgtga cccggaattg caggagactg
     7861 ttctccgcca ccgtcttgga acgaatcgaa tccaaaaccg gtgcccggtt cacgttcgtc
     7921 accgtgatcg tcaccaactc gctgtcggct aaagaaccat ccgaggcaat gaacctcacg
     7981 ttgtaaatcc ccgcctggaa gaaagagggc gtccaggtga atgagccccg accattcagc
     8041 gaatccacaa ataccgcccc caccggcaga ttcaccgctt gtaaagcggg aaccgtcgaa
     8101 tccggatccg acgaggtcac caggaactgc aagagactgt tctccgcaac gctcctggcg
     8161 cgaacagagt ctaagaccgg cgctcggttc acattcgtca ccgtgatcgc caccagctca
     8221 ctgtcagcta aggaaccatc tgacgcaatg aaggtgacgt tgtacgtccc cgcctggaag
     8281 aaagtcggcg tccaggtgaa actcccccgg ctgttcccgc tgtccacaaa caccgccccc
     8341 accggtaaat tgatcgcgct caaagaaggc gtggtcaaat ccggatcgct ggaggtcacc
     8401 cggaactgga gaagttgatt ctcggctacc gtcttggccc ggattgaatc cagaaccggt
     8461 gccaggttgc tattcgcgac ggtaatcgtc accacttcgc tgtcggccag ggcgccatcc
     8521 gacgcaatga acgtgacgtt ctaggtcccc gcctggaaga aggaaggcgt ccagctgaaa
     8581 ctcccgcgcc cgttcagtga atccacaaat accgcacccg ccggcaaacc caccgcagaa
     8641 agcgccggga gcgtcgaatc aggatcactg gacgttaccc ggaactgcag gagactgttc
     8701 tcggcaacac tcttagcgcg aattgagtct aagaccggtg cccgattgac gttcgtcacc
     8761 gtgatcgtca ccaactcgct gtcggctaaa gaaccgt
//
LOCUS       DBNGZD010000141         2028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_93659, whole genome shotgun sequence.
ACCESSION   DBNGZD010000141 DBNGZD010000000
VERSION     DBNGZD010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_93659"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC16GM_03850"
     CDS             <1..477
                     /locus_tag="AC16GM_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922843.1"
                     /translation="VLAWSLTFMFVNMLWTQALLAYDCQKKLAWVTLGNVLVNVGANL
                     VLIPIAGFRGACIATVLTEAVSFVLQGVIIRRLAGSLGILKATAQGAALAAGAVALET
                     AARSLSSGWLVTGIAVWSGLMLAFLFSLRGVLGRLPLFPFFRSPAQETVFPEVPWS"
     gene            468..1001
                     /locus_tag="AC16GM_03855"
     CDS             468..1001
                     /locus_tag="AC16GM_03855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2922844.1"
                     /translation="MVVVDFGCGRRKHPGTIGLDRNPRSDADIICDLNHPVPLPDSSV
                     DGVICKDVLEHVGDLVFTMEEIHRICKPGAKVEIQGPFASSPEFFHDPTHRRAFTSQS
                     FDYFIEGTPAAQFRYSDAYFRLEKVYYSRGRVRLLDRLFLSLANRFKRQFEARFLFWY
                     PVNTIHFVLRTVKNGKA"
     gene            988..>2028
                     /locus_tag="AC16GM_03860"
     CDS             988..>2028
                     /locus_tag="AC16GM_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2922845.1"
                     /translation="MGKRSRRIGIDVGPLTWPVQSGIPTFVRQLVSGLNSTPEAAAAG
                     AGAISFGYYRFSHRGWSPLAREFVTSGLRIAGLPLPMRLARWLWDFPGFLEVAGVKQY
                     DLVVGTVSELPPLAPQQKGLSVFHDLAGFKIPGAFSAPAVRQRRAIYQKTLERSHRIL
                     VYSRSTWRDLVELFGVPEEKLRLVPLGIELAPFQKKIGPEEKNRILRKYGISPPFIFF
                     SGIIQPRKNLARLAEAFLSLKEEEKLPHQLVLAGGLGYQGKEILEVIRSQDKSGAIHY
                     TGYVAASDLPLLYQAADLFVYPSLYEGFGLPVVEAMASGTPVVASYSSSIPEVVGQAG
                     VLVDPESPEAIAS"
BASE COUNT          351 a          617 c          609 g          451 t
ORIGIN      
        1 gtcctggcct ggtctttgac tttcatgttc gtcaacatgc tctggaccca ggcgctctta
       61 gcgtatgact gccagaaaaa gttggcctgg gtgactttgg ggaatgtgct cgtaaacgtc
      121 ggcgccaacc tggtgctcat accgatcgcc ggctttcgcg gtgcctgcat cgcgaccgtt
      181 ttgaccgaag cggtcagttt tgttttgcag ggggttatca tccgcagact ggccggttct
      241 ctgggcatat taaaggcgac cgcgcagggt gccgccctgg ccgccggggc cgtggcgctg
      301 gagacggccg cacgttcgct ctccagcggc tggctcgtaa cgggcattgc cgtctggagc
      361 gggctcatgc tggcgttcct cttttccttg cggggcgtgc tggggcgtct gccgctcttc
      421 ccgtttttcc gcagcccggc ccaagaaacg gtcttcccgg aggtgccatg gtcgtagtcg
      481 atttcgggtg cgggcggcgc aagcacccgg ggacgatcgg cctcgatcgc aatccccggt
      541 cggacgccga catcatctgc gatttgaacc atccggttcc cctgcccgac agctcggtgg
      601 acggggtgat ctgcaaagac gtgctggagc acgtcggcga tctggttttc accatggagg
      661 aaattcaccg gatctgcaag ccgggtgcga aagtggaaat ccagggaccc ttcgcctcca
      721 gccccgaatt ctttcacgac ccgacccacc gccgggcctt tacttcgcag tcgttcgact
      781 acttcatcga gggcaccccg gcggcccagt tccgctactc cgacgcctat ttccggctgg
      841 aaaaagtgta ctactcgcgc ggccgcgtgc ggttgttgga ccgcctgttc ctttctctgg
      901 cgaaccgctt caagcggcag ttcgaagcgc ggttcctctt ttggtacccg gtgaacacca
      961 tccacttcgt cctgcggacg gtgaaaaatg ggaaagcgta gccggaggat cggcatagac
     1021 gtgggacccc tcacctggcc ggtgcagtcg ggaatcccga ctttcgtgcg gcaactcgta
     1081 tccgggctca actcaactcc ggaagcagcc gcggccgggg ccggagccat ttccttcggt
     1141 tactatcgtt tctcccaccg ggggtggtcg cctttggcgc gagaatttgt caccagcggg
     1201 ctgaggattg cggggctgcc tttgcccatg cgtctggcgc gctggctctg ggacttcccc
     1261 ggatttctgg aggtggccgg agtgaaacag tacgacttgg tcgtcggcac cgtgtcggaa
     1321 ctgccgccgc ttgcgccgca gcagaaaggt ttgagcgtct tccacgacct ggccggcttc
     1381 aaaattccgg gtgctttttc cgcaccggct gtgcgccagc gccgggccat ctaccagaag
     1441 accttagaac gctcccaccg cattctggtc tactcccgct caacgtggcg ggacttggtg
     1501 gaactgtttg gggtccccga agagaaattg cgcttggtcc ccctgggaat cgagctggct
     1561 ccctttcaga agaaaatcgg cccggaagag aaaaatagga tcttgagaaa gtatggaatt
     1621 tccccaccct tcattttttt cagcggcatt attcaaccgc gcaagaattt ggcccgcctg
     1681 gccgaagctt tcctctcgct caaagaagaa gaaaagctgc cacaccagtt ggtgctcgct
     1741 ggcggtctgg gttaccaggg aaaagaaatt cttgaggtga tcaggtctca ggataaaagc
     1801 ggcgccatcc attacaccgg ttacgttgcc gccagtgacc tccccctcct ttaccaggcc
     1861 gcagaccttt tcgtttatcc ctccctgtat gaagggttcg gcctgcccgt cgtggaagcg
     1921 atggcctccg gcacaccggt ggtggcgtcc tatagttcat ccataccgga agtggtaggc
     1981 caggccggcg tcttggttga cccggaaagc ccggaagcga ttgccagc
//
LOCUS       DBNGZD010000142         4802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1482837, whole genome shotgun sequence.
ACCESSION   DBNGZD010000142 DBNGZD010000000
VERSION     DBNGZD010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1482837"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..1807)
                     /gene="rho"
                     /locus_tag="AC16GM_03865"
     CDS             complement(386..1807)
                     /gene="rho"
                     /locus_tag="AC16GM_03865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR2922846.1"
                     /translation="MKTVANGPAGRRNKVNADVAVAEPPEPQLAEVVTPTPGEEATTP
                     VIQVGEPPLSEKPELSITELKNKTIAQLLKTAEDLEIPGCSGLRKQELIFKILEAKTQ
                     KSGLIFAEGVLELMEEGYGFLRSPDYNYLPGPDDIYVSPSQIKRFDLRTGDTVSGQVR
                     PPKEGERYFALLKIEAVNFENPEVAKHKTLFDNLTPLYAQKRFKLETEQPEIAMRIMD
                     LLTPTGKGNRGLITSPPKAGKTILLQKIANSITTNHPEVKLIVLLIDERPEEVTDMKR
                     SVKGEVISSTFDEPAERHVQVAEMVIEKAKRLVEHQNDVVILLDSITRLARAYNAVVP
                     HSGKILSGGVDSNALQKPKRFFGAARNIEEGGSLTVIATALIETGSRMDEVIFEEFKG
                     TGNMEIVLDRRLSDRRIFPAIDINRSGTRKEELLLTPEELNRVWIMRKFLNEMNAIEA
                     MEFLQDRMRKTKDNAKFLESMKG"
     gene            complement(2224..2682)
                     /gene="rsfS"
                     /locus_tag="AC16GM_03870"
     CDS             complement(2224..2682)
                     /gene="rsfS"
                     /locus_tag="AC16GM_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR2922847.1"
                     /translation="MRRSPVARTGRHTAAARLPAAPKHRLTIARPSRSRASSGRLALL
                     AGQLALEKKARQVLVLDLRGLSAVCDFFVLCSADAHVHAQAVADNIVLRLEAKGQKVW
                     HKEGYPDTSWILLDYVDFVVHIFLDEARSFYNLERLWGDAPSRILDENHR"
     gene            complement(2672..3196)
                     /locus_tag="AC16GM_03875"
     CDS             complement(2672..3196)
                     /locus_tag="AC16GM_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIR2922848.1"
                     /translation="MRRKAASPRRAFRRRTVRSSRKTFWTKIAFGVLFIITVAYFASF
                     VKRIAQGESSDAPYPVRQARLQVLNGTGRPGLARKVADLYRRSTYQGVFFDVVDEGNS
                     APRVVEKTLVVNRTPEDLWGEATAAVLGVASRQVLTHPLTDNVLNLHVTVIVGGDIDS
                     LLRLQEQKKGERAS"
     gene            complement(3366..3839)
                     /locus_tag="AC16GM_03880"
     CDS             complement(3366..3839)
                     /locus_tag="AC16GM_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922849.1"
                     /translation="MKKVGLALSAVCFLSFLSTAVYSQGILQSQKPDLLRSPLQSGQG
                     LSLFAPSRFSFRNSYTLSFMSNGRQGNLLGLYVGSVNYQVAKPLEVTVNLGYLHQPQN
                     FVARNRQSGINGQILPSIRLDFRPSENFFFRIDVGSYPAWGGTPNLYDPYYFWNR"
     gene            complement(3999..4418)
                     /locus_tag="AC16GM_03885"
     CDS             complement(3999..4418)
                     /locus_tag="AC16GM_03885"
                     /inference="COORDINATES: protein motif:HMM:NF022591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HIR2922850.1"
                     /translation="MRKGMIGGLVLACGMVGTALAQEDTAKAMPAAAEVTVGHIVFGT
                     GIENREATGVDSSFAGDVGRVYCWTLITGASGETEVSHIWYHNDKEVAKVALPVRSPR
                     WRTYSSKTILPSTSGSWRVDVADASGNVLKSAKFEVK"
     gene            complement(4430..>4802)
                     /locus_tag="AC16GM_03890"
     CDS             complement(4430..>4802)
                     /locus_tag="AC16GM_03890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2922851.1"
                     /translation="SRAVIPIMQAQQSGQIVNVVSGAGRTGIPNLAAYCASKFGLLWF
                     GEALALELRPYHIKVASILPGSVDTEWSAEFPKDRPKPNPLPVKLTLEEVAEGILFLC
                     TQNENAWTSELNLRPHKVVAQ"
BASE COUNT          951 a         1414 c         1396 g         1041 t
ORIGIN      
        1 cgccagaggc ggattcgcac aaagaactgg tagtcgggtc gatacgactc gtgccgagct
       61 cgtccctgag cccgactctt gtctttgact ccgggacgag ccggtctttc gagttacgag
      121 gatcaaccct gggttgcaag ggctagagtg tcttccgcgg ctggaagcct cggctatgga
      181 agaggaaaaa ttccttcctt ccataggtca ggcatcctgc ctgaccgaag tccgagactc
      241 gtatagaccg ccaacgttgg tcagatacga cccgtaccgg gaaacctcgg ctacaaatat
      301 tcgggtggac gtaaacgtcc gcccctacga aggatttaca ccagctgctt ttgggggaac
      361 tgcgcgaaaa acgctgaagc cgggcttacc ctttcatcga ctccaaaaac ttggcgttgt
      421 ctttggtctt tctcatccgg tcctggagga actccatggc ctcgatggcg ttcatctcgt
      481 ttaagaattt gcgcatgatc cagacccggt tcagttcttc cggcgtcagg agcagttctt
      541 ctttgcgcgt gccggagcgg ttgatgtcga tggccgggaa aatccggcgg tcggaaagcc
      601 ttcggtccag gacgatctcc atgttcccgg tgcctttgaa ttcctcgaag atcacctcgt
      661 ccattcggct gccggtttcg atcagggccg tggcgatgac ggtcaaagac ccgccctctt
      721 cgatgttccg ggccgcgccg aagaagcgct tcggcttctg gagcgcgttg gaatcgaccc
      781 cgccggagag aatcttgccg gagtgcggca ccaccgcatt gtaggcccgg gccagccggg
      841 tgatggagtc aaggagaatc accacgtcgt tctggtgctc caccagccgc ttggccttct
      901 cgatcaccat ttccgccacc tgcacgtgcc gctccgccgg ctcgtcgaag gtggaggaaa
      961 tcacctcgcc cttcaccgag cgcttcatgt cggtcacctc ttcgggccgc tcgtcgatta
     1021 agagcacgat cagtttgact tccggatggt tggtggtaat cgagttggcg attttctgca
     1081 ggaggatggt cttccccgct ttgggcggcg aggtgatgag cccccggttt cccttgcccg
     1141 tgggggtcaa aaggtccatg atgcgcatcg cgatttcggg ctgctcggtt tcgagcttga
     1201 accgtttctg ggcgtacaga ggcgtcaggt tgtcgaagag cgttttgtgc ttggccactt
     1261 ccggattttc aaagttgacc gcctcgattt tcaaaagcgc aaagtagcgc tcgccttcct
     1321 tgggcggccg cacctggccg gagacggtgt cgccggtgcg cagatcgaag cgcttgatct
     1381 gcgagggaga gacgtagatg tcgtccggcc ccggcaggta gttgtagtcg ggtgagcgca
     1441 ggaagccgta cccttcttcc ataagttcca gaacaccctc ggcaaagatg aggccgcttt
     1501 tctgcgtttt ggcctctaag attttgaaaa tcaattcctg cttgcgcagg cccgaacagc
     1561 cggggatttc taagtcctcg gcggtcttca agagttgggc gatggtcttg tttttgagtt
     1621 cggtgatgga gagttccggc ttttcggaaa gcggcggttc ccccacttga atgacggggg
     1681 tggttgcttc ttcgcccggg gttggggtta cgacctcagc gagttggggc tcgggcggct
     1741 cggcgacggc cacgtcggca ttcaccttgt tccggcggcc ggccggccca ttagcgacag
     1801 ttttcatggt ttcctttgta gcggtagaag gtttacggtt gttcacggta gcgcggtact
     1861 gcggttgtct tgtgaatcag ccctgctggg attatcgact gcgtacgaga ttactttatg
     1921 ctctggtttc cggcccatgc aagggtatag gtctctgccc tgcatcttcc ggaggttggt
     1981 accacccgta ccgaagagga ttctgcctgt ataacgcacc tacccacgtc tatgtttgag
     2041 gcaacatctt tctttccggt gcctgggcaa cataaaaggg agaataaaga atgtcaagaa
     2101 tttttttgct ttccggccgg aaatcttttc tgccccggtt gcatcatgtg cgagccgcac
     2161 ggccgctcgc tggactgtgg gtggctacgc tatcagaggt ccataccccg gacgccaaca
     2221 ggatcaccgg tgattctcgt ctaagattcg gctcggcgca tccccccaca accgttccag
     2281 gttgtagaac gaccgcgctt cgtccaggaa aatgtgcacg acgaaatcga cgtagtctaa
     2341 gagaatccag gatgtatccg ggtacccctc tttgtgccag accttttgcc ctttcgcttc
     2401 caggcggagg acgatgttat ccgccaccgc ctgggcatgc acgtgcgcat cggccgagca
     2461 aagaacgaag aaatcacaca ccgcggagag gccgcgtaaa tcaagcacca gcacctgccg
     2521 cgcctttttt tctaaagcca gctggccggc caaaagggcc aaacgcccgg aagaggcccg
     2581 ggaacgggac ggtcgggcga tggtcaggcg gtgtttgggt gccgcgggca ggcgtgccgc
     2641 cgcggtatgc cggccggttc gggccaccgg actacgacgc acgttcgccc tttttctgtt
     2701 cctgcaggcg caggagcgaa tcgatgtctc cgccgacgat caccgtcacg tgcaggttca
     2761 aaacgttgtc cgtcaaaggg tgggtgagca cctggcggga ggccacgccc agaaccgccg
     2821 ccgtggcttc gccccagaga tcttccggcg tgcggttcac caccagagtt ttttcgacga
     2881 cgcggggcgc cgagttgcct tcgtccacca cgtcgaagaa aaccccctgg taggtggagc
     2941 ggcggtacag gtcggccacc ttccgggcca ggcccgggcg gcctgtaccg ttcaacacct
     3001 gcagccgcgc ctgccgcacc gggtagggcg catcgctgga ctcaccctgc gcgatccttt
     3061 tgacaaacga agcgaagtag gccaccgtga tgatgaagag caccccgaaa gcgattttcg
     3121 tccaaaaggt ttttcgggaa cttctcaccg tccggcgtct gaaggcccgg cgcggggaag
     3181 cagctttccg gcgcaaaagg ggaatcattc gctccggaag tcctccgggc ggaaattgag
     3241 tcgagacaaa tatgcagcga acagagtgct aaacgacaac agacgatggg cgcgatggca
     3301 ggagtggatt tggcagcccc gggtgcgtgg tgacttttgg tccgtcttgg cctgctcctt
     3361 cgcttttagc ggttccagaa gtagtacgga tcgtaaaggt tcggcgtccc gccccaggcg
     3421 gggtaactgc cgacgtcaat ccggaaaaag aaattctccg acggccgaaa atcgagccgg
     3481 atcgagggga gaatttgtcc gttaatgccc gactgccgat tacgggcgac aaaattctgc
     3541 ggctggtgca ggtagcccaa gttgaccgta acctccagcg gcttggcgac ctggtagttc
     3601 accgaaccga cgtaaagccc caaaaggttc ccctgcctcc cgttggacat aaacgagagg
     3661 gtataactgt tgcggaacga gaaccgtgac ggggcaaaaa gcgacaaccc ctgcccggac
     3721 tgcaacggcg aacgcagcag gtcgggtttt tgcgactgta aaattccctg agagtagacc
     3781 gccgtgctga gaaaactcaa aaagcaaact gcgctgagtg ccaaaccgac ttttttcatt
     3841 ccacgctcct cgaaagaggg ctactactcg tctttacttt aaatatacgc aaacccggca
     3901 gcaccggtgg cacaaataat gtcaaagacc acgctctgtc aatccttaat ctctatttcg
     3961 gccggtcgcc gggcggcctg aatcccgcct gaggcgaatt acttgacctc aaacttggcg
     4021 ctcttgagca cgttgcccga ggcgtccgcc acatccaccc gccaggagcc ggaggtcgac
     4081 ggcagaatgg ttttggacga ataagtccgc cagcggggcg agcgcaccgg taaagccacc
     4141 ttcgccactt ccttgtcgtt gtgataccag atgtggctca cttcggtttc gccggaggcg
     4201 ccggtgatca aagtccagca gtagacccga ccaacatctc ccgcaaacga cgagtcgact
     4261 ccggttgcct cccggttttc gatgccggtg ccgaagacga tgtgccctac ggtgacttca
     4321 gccgcggctg gcatggcctt ggcagtgtct tcctgcgcca aggccgttcc gaccatcccg
     4381 caggccaaaa cgagtccccc gatcattccc ttccgcataa accctccctt cactgcgcca
     4441 ccaccttgtg cggtctcaaa ttcaattctg acgtccaggc attttcgttt tgcgtgcaga
     4501 ggaacagaat cccttcggcc acttcctcca gcgtcaattt caccggcaag gggttcggct
     4561 tgggccggtc tttgggaaat tccgccgacc actccgtatc gaccgaaccg ggtaaaatcg
     4621 acgctacctt aatatgatat ggccgcaact ccagcgccag cgcctccccg aaccacaata
     4681 gcccgaactt tgacgcgcag taggccgcta aattgggaat gccggtcctt cccgcgccgg
     4741 agacgacatt gacgatttgc ccgctttgct gcgcctgcat tatcggaatg accgcccgcg
     4801 aa
//
LOCUS       DBNGZD010000143         3487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1343962, whole genome shotgun sequence.
ACCESSION   DBNGZD010000143 DBNGZD010000000
VERSION     DBNGZD010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1343962"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="AC16GM_03895"
     CDS             <1..1212
                     /locus_tag="AC16GM_03895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2922852.1"
                     /translation="EEATGRSLGWFFDQWVYKGGYPRFEVHADWDEEHKVEVVKVKQT
                     QTPDTLTPVFRAPLIIELVDSSGTRSNPVEITQADQQFTFPASRRPLEVRFDPGNTIL
                     KTLDFPKEKKELFYQVRFDPDVVARIWACGELGKKFADPEVVDTLAAVLTGDSFYGVR
                     GAAASALGKLQTGAAEKTLYVGYNDKDARVRRAAVDALGEFKESQTATDFLKKVIAGD
                     PNDYVKASAVSSIGRIAAPGAFEILKQALRQPSEYDVVRTAAAGAFGTLKDKKAIDLL
                     MPETRYGKTPSVRTAAIGALGKVGKEEDSVTRRLLLLLNDPWLWVRGSAMDALAELKI
                     AEAIPAIEGHAQNEPDGRLRKQARNAIKRIREGKAETEDMAALRSELERLKSEQKLLR
                     DRIEVLEKHAP"
     gene            complement(1303..1374)
                     /locus_tag="AC16GM_03900"
     tRNA            complement(1303..1374)
                     /locus_tag="AC16GM_03900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1339..1341),aa:Arg,seq:ccg)
     gene            1572..3305
                     /locus_tag="AC16GM_03905"
     CDS             1572..3305
                     /locus_tag="AC16GM_03905"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR2922853.1"
                     /translation="MRWSELYIPTLRDDPADAELVSHKLLVRAGYVRKLTSGVYVYLP
                     LMQRVLAKISQIVREEMNKKGAQEITMPVLHPAELWQASGRWESIGKELFRLKDRHQR
                     EMALGPTHEEIVTNLVKGEIRSYRQLPLNLYQIQVKFRDEIRPRFGLMRCREFPMKDA
                     YTFDASEESFKQSYQKMLDAYRSAFLRCGLETKMVESDTGAMGGKAAHEFVVIVETDG
                     GESEILFCQKCDYAAHIDKAMSVEPNPPAADGKPKPNQKVDTPGQKTVEEVSRFLGVT
                     PRDLVKTLLYRADGKIVAALIRGDRQINEVKLKNALGVLELEMADAATVEKVSRAPIG
                     FAGPVGLSGATFVCDPEVTKLVNFVTGANEADRHLINVNINRDFAPDRVADIRNAAPG
                     EFCPRCQQGVLQSARGIEVGNTFQLGTKYSESLGATFIDEHGQEKPFIMGSYGIGITR
                     TTQAAIEKFHDQAGIIWPLSIAPFAVEILPVNPGDEKQKSLALSLYEELTKQRIEVLL
                     DDREERAGVKFKDADLIGIPLRITIGEKSLAENMVEIKIRKTGETIKPAVPQAVTKTN
                     EVLSRLAPDLG"
BASE COUNT          847 a          921 c         1038 g          681 t
ORIGIN      
        1 gaggaggcca ccggccgctc tttgggttgg ttcttcgacc agtgggttta taaaggcggc
       61 tatcccaggt tcgaagtcca cgccgactgg gatgaggagc acaaagtcga agtcgtcaag
      121 gtcaagcaga cgcagacgcc cgacacgttg acgccagttt tccgcgcacc gctgataatc
      181 gaactcgtgg acagcagcgg aacgcgctcg aatcccgtgg agatcactca ggccgatcag
      241 cagttcacct tcccagcttc gcgacggccg ctggaagtac gctttgatcc ggggaatacc
      301 atcctcaaaa ctctggactt tcccaaagaa aaaaaagaac tcttttacca ggtgcgcttt
      361 gacccggacg tggtagcccg catctgggcc tgcggggagt tgggaaagaa gtttgccgat
      421 ccggaagtgg tcgatacgct cgcggctgtt ttgaccggag attcattcta cggcgtgcga
      481 ggcgcagcgg cttcggcctt gggaaaactt cagacgggcg cggcggagaa aaccctctac
      541 gttgggtata acgacaaaga cgcccgcgtc cgccgggcgg cggtcgatgc gttgggggaa
      601 ttcaaagaga gccagaccgc caccgacttc ttgaaaaaag ttatcgccgg ggatcccaac
      661 gactacgtga aggccagcgc tgtctcctca attggtcgga ttgccgcgcc gggtgcgttt
      721 gagattttga agcaagccct gcgccaaccc tcagagtacg acgtggtgcg cacggcagct
      781 gccggtgcct ttggaacgct gaaagacaag aaggccatcg acctgcttat gcccgagacg
      841 cgctacggca aaacgccttc agtccgcacg gcggccattg gcgcgttggg gaaagtaggg
      901 aaggaggagg atagcgtcac ccggcggctt ctcttgcttt tgaacgaccc ctggctgtgg
      961 gtgcgtgggt cagcgatgga tgctttagcc gaattgaaga ttgcggaggc aattccggca
     1021 atcgaaggtc acgcacagaa cgaacccgac ggccgcctgc gcaagcaggc gcggaatgcc
     1081 attaaaagga taagagaagg aaaagccgag accgaagaca tggccgccct gcgcagcgag
     1141 ctggaacgtt tgaaatctga gcagaaactt ttgcgcgacc ggattgaggt cttagagaag
     1201 cacgcgccct aaaaaaagtg tcgaagtatc gatgtgttga ggtgttggag ttaaaagatt
     1261 ttaccgatcc ctaatccctg atctctgatc cctgcagtta catacccccg gtaggaatcg
     1321 aacctacatg gccagctccg gaggctggtg cgttgtccat tacgcgacgg gggcgaataa
     1381 aagcagtgat gagtgacaag tgattagtga tggaaagaaa agcgctcatc gtatctccaa
     1441 aatatacttg agaagaattt agtccggagg gggaatgaag tcaatggcat ttcaagcgag
     1501 tttgacttca cctgctgcac acatataatt acgcttagcg tcacttagtt accaattacg
     1561 agttacaaac catgcgctgg agcgaactgt acatccccac cttgcgagac gacccggccg
     1621 atgccgaact ggtcagccat aagctgttag tccgggccgg gtacgtccgc aaactgacct
     1681 cgggggtgta cgtctacctg cctttgatgc agcgcgtcct ggccaaaatt tctcagatcg
     1741 tgcgggagga gatgaataag aaaggggcgc aggagatcac gatgccggtg ctgcacccgg
     1801 cggaactctg gcaggcctcc ggcaggtggg agtccatcgg caaggaattg ttccggctga
     1861 aagaccgcca ccagcgcgaa atggccttgg gcccgacgca tgaagaaatc gtcaccaacc
     1921 tggtcaaagg tgaaatccgc tcctaccggc aattgccctt gaacctgtac cagattcagg
     1981 tgaaatttcg ggacgagatc cgcccccgct tcggtttgat gcgctgccgg gagttcccga
     2041 tgaaagacgc ctacacgttc gacgccagcg aggaaagttt caaacagagt taccagaaaa
     2101 tgctcgacgc ctaccggagc gcctttctgc gctgcgggct ggaaacgaaa atggtcgaat
     2161 ccgacaccgg cgcgatgggg gggaaggcgg cccacgaatt cgtggtgatc gtcgaaaccg
     2221 acggcggcga gagcgaaatt ttgttctgcc aaaaatgcga ctacgccgcc cacatcgaca
     2281 aagcgatgtc ggtcgagcca aatccaccgg cagcggacgg aaaacccaaa ccaaaccaga
     2341 aagtcgatac ccccggacaa aagaccgtgg aagaggtgag ccgctttctg ggcgtgacgc
     2401 cgcgcgactt agtgaaaact cttctctacc gggccgacgg caaaattgtg gcggctttga
     2461 tccgcggcga ccggcagatt aacgaagtga aattaaaaaa tgccctggga gtccttgaac
     2521 tggaaatggc cgatgcggcc acggttgaaa aagtctccag agcgccgatc ggttttgccg
     2581 ggccggtggg actttccggg gcaacttttg tctgcgatcc ggaagtgacg aaactggtta
     2641 acttcgtgac cggcgctaac gaagccgacc ggcacttgat caacgtcaac atcaaccgcg
     2701 actttgcacc cgatcgggtg gccgacatcc gcaacgcggc acccggtgaa ttctgccccc
     2761 gctgtcagca aggcgtcctg cagtcggcgc ggggtattga agtcggcaac acctttcagt
     2821 tgggaaccaa gtacagcgaa tcgctcggtg cgaccttcat cgatgagcac gggcaggaaa
     2881 agcccttcat catgggctcc tacgggatcg gcatcacccg caccactcag gcggccattg
     2941 aaaagtttca cgaccaggcc gggattatct ggccgctctc gattgcgccc ttcgccgttg
     3001 agattttgcc ggtgaacccc ggcgacgaaa agcagaaatc gctggcgctg tcgctctatg
     3061 aggaattgac caaacagaga attgaggtgc tgctggacga tcgggaggag cgggccggcg
     3121 tcaaattcaa agacgccgat ttgatcggca ttccactgcg aatcactatt ggcgaaaaat
     3181 ccctggccga aaatatggtg gaaatcaaaa tccgcaaaac gggagagact ataaaacccg
     3241 ctgtacctca agcggttaca aagacgaacg aggttttgtc ccggctcgcc ccggatttgg
     3301 gttgattttt ttttcttggg gtggtaaatt agtgtgttcg gtctggaagt gggcggtggc
     3361 ccactttttg gtattgtgtt tacagccggg ccacgaccct cggggcaaat gaacctgacc
     3421 gtgaggcatg ttccggcaag tgtgggaaaa tggaagggat acgcgaaaga cttgtggaaa
     3481 ttatcgc
//
LOCUS       DBNGZD010000144         3091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_648695, whole genome shotgun sequence.
ACCESSION   DBNGZD010000144 DBNGZD010000000
VERSION     DBNGZD010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_648695"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(541..1869)
                     /gene="tolB"
                     /locus_tag="AC16GM_03910"
     CDS             complement(541..1869)
                     /gene="tolB"
                     /locus_tag="AC16GM_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR2922854.1"
                     /translation="MKILRSICFGLLCLGVFSASSVAQQELRLRLRVPGFKPIEVGVD
                     DFASDSRIFGPGDQELLTKIPQIIRDDLWYALFFVVIEPDTAYLRTFQKEKLEPADWF
                     ALGAKFYLSGKLNFKADRITCDFELRDVNTNHKIVSDDVSQARGQERRIAHKIADAIV
                     KQFTGKDGIFSTQLAFVSAQSKNREIFVCDFDGANVRKVTDDRSINLSPSFSPDGTKL
                     LYTSYKAGNPDLWLLDLRSARSKKISAKAGLNSAAVWSPDGRSIAATLSYQGNPELYL
                     LDANGNIIRRLTQNEAIDSSPSFSPDASQVAFVSDRSGAPQIYLADVETGAVNRLTFA
                     GSYNDSPAWSPDGTQVALVMRNDQGNFDVCTVDVTGQNYRQLTSNGSNENPHWSPDGL
                     HLVFSSNRSGIFQIYTMDFDGNNQRMITSRNESNPTGGQNYNPTWGPYLK"
     gene            complement(1877..2494)
                     /locus_tag="AC16GM_03915"
     CDS             complement(1877..2494)
                     /locus_tag="AC16GM_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR2922855.1"
                     /translation="MKGEFVVSALAHLLLCVILFTGGTSRPLIVARPQPIQVRLVSLP
                     PEPPEAVAEAKPIKKPPVKETKGITFAEIKKPKKKAEKKAAAKVEEPKPQPQVSPSQT
                     GSRFGEAVVDAPDFAYSYYVDLILNKIYDGWANPVSSDFALSVVIYFKILRGGQLADV
                     KVEAPSGIAAFDRAAYRAVDNAGPLPPLPAEYEGTELGIHLEFQQ"
     gene            complement(2491..2910)
                     /locus_tag="AC16GM_03920"
     CDS             complement(2491..2910)
                     /locus_tag="AC16GM_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR2922856.1"
                     /translation="MELSENRRYHPRTPLADINVTNLVDVVLVLLIIFMITAPMLQSG
                     IDINLPKAKTEAKEVSESATVTITKERGIFINDKFVRPDRFETQLGVLKRSGQVRSIL
                     LRADKDVPYGFVVETMAKIRAAGIENLNLITVPEERK"
     gene            complement(2901..>3091)
                     /locus_tag="AC16GM_03925"
     CDS             complement(2901..>3091)
                     /locus_tag="AC16GM_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922857.1"
                     /translation="GGSPLQKDEKNKAGRPGGSPLRNDRTALGWETQPLHCETQPLQE
                     NDPSSPYGKTDRMDLTWN"
BASE COUNT          588 a          894 c          910 g          699 t
ORIGIN      
        1 ggcgtgggtg gcggtgccgg tgttggcgtt ggggccggtg ccggcggtgg tggtggcggc
       61 gctttcttgg cgcagccgac cataagcccg ctggctgcca agctgagcat gagcaaaatc
      121 gctgtcagtt tccgcatcgg gttcctcctt gatttttcgc gttcaaattc cctcgcttga
      181 ctcataataa accacgccgc tcgtggctgt caacagaaaa atgctcgccc gcatcgctgt
      241 agcgcaggtt ggttcgccct gagcgaagcc gaagggttta cttgtgccga gtccgcctca
      301 ggtggatcga ggcatggaac ctgcgccatt ttttattcgc cccggtagca caggcccttg
      361 tggccgttcc gaggctggtc tgatacgact ctacgagaag cctcggctat ggaggcaaga
      421 aatgtctttt atccctcggt caggcttctc ggtgagagtc gtgccgacca gcctgacccg
      481 aattctgatt tctgtacgat tccccccaaa tcccgactcc cttatcccaa atccgctttt
      541 ttatttcaag tacggccccc acgtgggatt gtaattctgc cccccggtcg gattcgactc
      601 gttccgagag gtgatcatcc gctggttgtt gccgtcaaaa tccatcgtgt aaatctgaaa
      661 gatgccgctc cggttggaac tgaagacgag gtgcaatccg tcgggcgacc agtgcgggtt
      721 ttcgttcgag ccgttggagg tgagctgccg gtaattctgt ccggtgacgt caacggtgca
      781 aacatcaaaa ttcccctgat cgttgcgcat caccagcgcc acctgcgtgc cgtcgggcga
      841 ccaggccggc gaatcattgt aactgcctgc gaaagtcaga cggttgaccg cgccggtttc
      901 cacgtcggct aagtaaatct gcggcgcgcc ggagcggtcc gacacgaagg ccacctgact
      961 ggcgtccggc gaaaacgaag gtgaggaatc aatcgcctca ttctgcgtca ggcggcggat
     1021 gatgttgcca ttggcgtcca aaaggtacag ttccggattc ccctggtaac tcaaggtcgc
     1081 ggcgatggag cggccgtccg gcgaccagac cgccgcgctg ttcaaccccg ctttggccga
     1141 gatttttttc gagcgagccg accgcaagtc cagaagccac aggtctgggt tgcccgcttt
     1201 gtacgaggtg taaagtaact tcgtcccgtc cggcgaaaag gacggtgaca ggttgatcga
     1261 acggtcatcg gtgactttgc ggacgttggc gccgtcaaaa tcgcagacaa aaatctcccg
     1321 gttcttcgac tgcgccgaaa cgaaggcgag ctgcgtggag aaaattccgt ctttgccggt
     1381 gaactgcttg acgatggcgt cggcgatctt atgcgcgatc cgccgctcct gcccccgcgc
     1441 ctgcgacacg tcgtcggaga cgatcttgtg attggtgttc acgtcccgga gttcaaagtc
     1501 gcaggtaatc cggtcggctt tgaagttcag tttaccggag aggtagaatt tcgcgcctaa
     1561 ggcgaaccaa tccgccggct ccagtttttc tttttggaag gtccgcaggt aagcggtgtc
     1621 cggttcgatc acgacgaaga agagcgcgta ccagaggtcg tcccggatga tctggggaat
     1681 tttggtcaga agttcctggt cccccgggcc gaagatgcgc gaatcggagg caaaatcgtc
     1741 cacgccgacc tcaatcggct tgaatcccgg cacccgcagg cgcagccgca actcctgctg
     1801 cgctacagac gaggcggaaa agaccccgag acagaggaga ccgaaacata tgctcctgag
     1861 gattttcatc agatcgctac tgctgaaatt cgaggtgaat gcccagctcc gtgccttcgt
     1921 actctgccgg caaaggcggg agcggcccgg cgttgtccac cgcccggtag gcggcccggt
     1981 cgaacgccgc gatgcccgag ggcgcttcca ccttgacgtc ggccagctgg ccgccgcgca
     2041 gaattttgaa gtagatgacg accgaaaggg caaagtcgct ggagaccgga ttggcccagc
     2101 cgtcgtaaat tttgttcaag atgagatcga cgtagtagga ataggcgaaa tcgggggcat
     2161 ccaccaccgc ttcgccgaaa cgtgagccgg tttgacttgg actgacctgc ggttggggct
     2221 tcggttcttc aactttggct gcggctttct tctccgcttt tttctttggt ttctttattt
     2281 cggcaaaggt gatccctttg gtttccttta ccggcggctt tttgatcggc ttggcctccg
     2341 cgaccgcttc cggcggttcg ggtggcagag agaccagccg cacctggatc ggctgcggcc
     2401 gggcgacgat caagggccgg gatgtgccgc cggtgaacag aatgacgcag agtaaaagat
     2461 gcgccagagc agaaacgacg aactcccctt tcacttccgc tcctccggca ccgtgatcag
     2521 gttcagattt tcgatccccg ccgcccggat tttggccatc gtctccacca caaagccgta
     2581 cggcacgtcc ttgtccgccc gcaaaagaat cgaacgcacc tggccgctgc gcttgagcac
     2641 gcccagctgc gtttcaaaac ggtcgggccg cacgaacttg tcgttgatga atattccccg
     2701 ctccttggta atcgtcaccg tcgccgactc gctcacctct ttggcttcgg ttttggcttt
     2761 ggggagatta atgtcgatcc cgctctggag catcggcgcg gtgatcatga agatgatcaa
     2821 aagcaccaag acgacatcga ccagattggt gacgttgatg tcggctaagg gcgtccgcgg
     2881 gtgatacctt ctattttcgc ttaattccac gtcaaatcca tcctgtcagt cttgccgtaa
     2941 ggtgatgacg ggtcattctc ttgtaggggc tgggtctcac aatgtagggg ctgggtctcc
     3001 cagcccagag cggttctatc attccgtagg ggcgacccgc cgggtcgccc ggccttgttc
     3061 ttttcatcct tctgtagggg cgaacccccg t
//
LOCUS       DBNGZD010000145        12455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_441292, whole genome shotgun sequence.
ACCESSION   DBNGZD010000145 DBNGZD010000000
VERSION     DBNGZD010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12455
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_441292"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="AC16GM_03930"
     CDS             <1..814
                     /locus_tag="AC16GM_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR2922858.1"
                     /translation="GPLEAPPLVYGSVVYIPVRGLGPGLVAYDVVAGSEQWRANLKDV
                     ATTPILYKSRLCAASAAGEVKGLDVAKGEELWHFDAGGPVLAPPIAYESTTVIGSVGG
                     KLHLLAMSTGKVIWEKEMPEGVRAQPAVVDSTLYVGGLDGNCYALDVTTGTTRWRTAV
                     GGKIYSSPVVADSLVFFGCNDRKVYALAQRSGAVVWTHTAGSIVNVPVTPVGKYLICG
                     TTDGTLFALQSDDGVLVWEYQLKGGLAGRPVGSGNQLFVTTTPGRVFCFTLR"
     gene            820..3048
                     /locus_tag="AC16GM_03935"
     CDS             820..3048
                     /locus_tag="AC16GM_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2922859.1"
                     /translation="MTSVAIFREEELRTNLRDLAIEVLRELWMSSRKVGLYSFDHPGV
                     QRAAAKPFFYLVRYFKYKSAFSCVIQDRRLFVGGTPVPPLVFTKGLVAEWERAAVDSV
                     LFRSEVTVNDLAVFLDRLSKRFPPSDYHNQYLQYLTKRKVFSIEINGDLAKKIFSRSN
                     LYDPELEYDFSVEGMAAELLRREAALATALLAGEWKSDREALEEGGVEFRLAVLREFA
                     GAVIGTYSEEKLLALVESQISGEDLNLLIEKSERVAALKNLLRSLAQHPQKDKVFGKV
                     KELFTARGVQREIYEQTFDLAALFRFRSSEETEALINKVFSSAFAPDDFERFREVFPR
                     LVASRQEEKLHEIFRVLLLNLTREDHSARQKALFLIRPTLESLDTLRDEGNFDFLIDD
                     LLTFVAEKKETFEFSEVFDSCLKICLKNRRYQPVAKMALALRQRLGGQNPDDAFGNVV
                     AQKALEFFGADAVVEQLVGEIVEGKTTQIQAIRQALTAINNQKVALALAEVVTHPDRA
                     VRQNALKILLHLGSNSLETFSRILNDGQTFIRQPDGNTLTQAAWFRVRNAIHVLGNLR
                     DERALEVLGRSAIDPDPRVRGEVVRALEKIGGTKAGIILKQLASDRDGEVRKTAIITL
                     GLVGTPDDVIFLRELFLLGSPERLPIFYAAGNLGGVQAKAFFLELLQPNSAAARLAFG
                     RGDDVQVKAAIVKGLAKVNDPECAEKVRELGDSLTNTQKVFIKNILSSKTPKKILSRL
                     MQ"
     gene            3315..3770
                     /locus_tag="AC16GM_03940"
     CDS             3315..3770
                     /locus_tag="AC16GM_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR2922860.1"
                     /translation="MALVRWRPARELSLFPEDFNRMFEDFWGRFPRFFGNDFGDGGTW
                     APAVDVTETKDDVIVKAEVPGMTKDDIKVTLQDNILTIQGEKKEEREEKEASFHRVER
                     SYGSFVRSFSLPTTVKSDKIHAAYKDGVLRITLPKVEEVKPKEIAVSVN"
     gene            3955..4866
                     /locus_tag="AC16GM_03945"
     CDS             3955..4866
                     /locus_tag="AC16GM_03945"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF053157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR2922861.1"
                     /translation="MTRDWEVQFREWAKPLGQTEQDRCDNAVSAIRNAIKASEKLRAR
                     GVLPSSVFAQGSYRNNTNVRQDSDVDIGILCTASFCYVLPEHTTAETFGIIPASYLYG
                     QYKNEVEEALVDYFGRSAVKRGNKAFDISETSYHVEADVAAFFEHRSYHSDGTYLKPE
                     GVALRTDKENRRVINWPDLHYENGVNKNKQIGTRFKSFVRILKALSIEMTEQGVRAGN
                     IPGFLLECLVWNVPNDRFQHNTYTDGVKAVLVFLYESTKTDELCREWVEISGLKRLFR
                     PTQKWTREQANAFTMAGWNYAGLGGQS"
     gene            4863..5537
                     /locus_tag="AC16GM_03950"
     CDS             4863..5537
                     /locus_tag="AC16GM_03950"
                     /inference="COORDINATES: protein motif:HMM:NF037740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cap15 family cyclic dinucleotide receptor
                     domain-containing protein"
                     /protein_id="HIR2922862.1"
                     /translation="MKNFNTKPFIYFLAVLSVVVLFVAALIQGFDLGNFLDVLRLIPI
                     VATVDVIAYFVFAGWLWRWKRLQGWLIPFPDLNGTWHGNIQTNWKNADGNTPRPIPAI
                     LTIKQTFGRMSCVMRTGEMESHSYLEGFCIDKDAQVRRLCYSYTSRPKAALRERSTPH
                     DGTILFNVVGNPVNKLEGEYWTQRQTAGTVTLTFRTKELVDEIPDDLPAHPITGGRGH
                     IVNDKF"
     gene            5614..7524
                     /gene="dnaK"
                     /locus_tag="AC16GM_03955"
     CDS             5614..7524
                     /gene="dnaK"
                     /locus_tag="AC16GM_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR2922863.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGQPVVIANSEGMRTTPSVVAFTK
                     TGERLVGQVAKRQAITNPLNTIFSIKRFMGRKFREVTEEIKNFPYKVVEGPNGDARVQ
                     VEKEQYSPPEISAMVLGKMKQTAEDYLGEKITEAVITVPAYFNDAQRQATKDAGRIAG
                     LDVKRIINEPTAASLAYGLDKKKNEKIAVYDLGGGTFDISILELGDGVFEVRSTNGDT
                     HLGGDDFDKRIIDWLVAEFKKQEGIDLSRDPMALQRLKEAAEKGKMELSSTMETQINL
                     PFITADQTGPKHLNVTLTRAKMEQLVDDLIERTVEPCRKALADAKLTVDQIDEVVLVG
                     GMTRMPKVQQLVRDFFKKEPHKGVNPDEVVAVGAGIQGGVLVGDVKDIVLLDVTPLSL
                     GIETLGGVFTRLIERNTTIPTRKSEIFSTAENNQTKVEVHVLQGERQMAIDNKTIGRF
                     ILDGIPPAPRGIPQIEVTFDIDANGILHVTAKDKATGKEQKIRIEASSGLTEAEIKKM
                     VTDAKEHEGEDKKRREEVDTRNRADQLAYETEKNLKELEGKIEPDDKAKIEAAVGRVK
                     EALKGKNTEEIRSAQEALTQVWHQASAKIYQKTAGQSAPGGSTGGTEPPPGGDKGPEG
                     GQPVDADYEVVK"
     gene            7549..7854
                     /locus_tag="AC16GM_03960"
     CDS             7549..7854
                     /locus_tag="AC16GM_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR2922864.1"
                     /translation="MPTYEYRCTANHRFEEVQNISEPPIEKCPHCGAKAQRVISGGAG
                     LLFKGSGFYITDYRSESYKKKAKEESGESNPPKADSGGETKLSETKPEKPKKTEGSP"
     gene            7907..8329
                     /locus_tag="AC16GM_03965"
     CDS             7907..8329
                     /locus_tag="AC16GM_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922865.1"
                     /translation="MPLPLMRVPGKLSSRSILSGAATFWLGMALTIWLSACSKRPSEK
                     ELFTQAQMAVTEQRYYTGLDLYAQVLRNFPKSENAYKALFMLGYVNSEFLHRPTVARD
                     YFKKVLEKYPGCDLADDAVYLLDNQSGLARPLPNPTTP"
     gene            8431..8907
                     /gene="dtd"
                     /locus_tag="AC16GM_03970"
     CDS             8431..8907
                     /gene="dtd"
                     /locus_tag="AC16GM_03970"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR2922866.1"
                     /translation="MRLVLQRVSRARVTVGGNVTGEIGPGLLILLGAKRDDTGKQADF
                     LADKVLGLRIFEDQAGKMNLSVRDVRGEILVVSQFTLYADTRRGRRPSFTEALEPQQA
                     EELYGHFVNRLKASGLVVATGSFGAKMEVELVNAGPVTLVLEKEEGKAEAEAERSA"
     gene            8925..9638
                     /locus_tag="AC16GM_03975"
     CDS             8925..9638
                     /locus_tag="AC16GM_03975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR2922867.1"
                     /translation="MNFVRPQTYTRFAEKIKLLLRGDRLILASSSPRRAQMLAELGLS
                     FFALRPRLVEEDLVIACPKARSVDLSGRKAASVLPEASAGLVLGADTSVVRGGEILGK
                     PKDHHEAFEMLHRLSGQWHEVYTGLSLVAAPSGAASSGWECTRVKFRALTARAIHEYI
                     ASGEPFDKAGAYGIQGLGGALVEGIEGDYENVIGLPVATFAFLLEQLRLTGPPNASVP
                     GAPHGELLADRGIPGEGSG"
     gene            9635..10393
                     /locus_tag="AC16GM_03980"
     CDS             9635..10393
                     /locus_tag="AC16GM_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2922868.1"
                     /translation="MTSIGQFFKEKREALGLSLEEMAERTRLQVSYLAALEGDRLDEL
                     PGRPYYRLFLRTYAASLGLNFDQIAREFPETEITAEIRLPPAQTVKQRARRAGIAVAS
                     VVVFAGILFFFLRYGRFSTTGPGQVEKSATETKSAGPKVAPPPARESTEVALQPPLVL
                     RLEGLEDTGVRVVADRDTLFNGLLKKAEVEQWFAQDSFIITLGKAWAVTAFVNEHKLK
                     RLGRPGEPRYRLVIRADNYERLVDSTALSEPGQE"
     gene            10404..>12455
                     /gene="smc"
                     /locus_tag="AC16GM_03985"
     CDS             10404..>12455
                     /gene="smc"
                     /locus_tag="AC16GM_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR2922869.1"
                     /translation="MYLSRVEIFGFKSFPQRTVLAFDGGITAIVGPNGCGKTNILDAI
                     RWSMGEQRPTVLRGTKMDEVIFNGSRSLKALGLAEVTLSIQNDRGVLPSEYEEVAVTR
                     RLLRTGESEYLLNQIPCRLKDITDLFLDTGMGAHAYSVIHQEMVDLILSSKSSDRREL
                     FEEAAGVAKYKQRRTEALRKLDTTQADLLRLSDLSAEVEKQVTSLKRQVKKAERHQRY
                     TAEIKTLELLRSRHEYFQLRRRRDVAAQEARELEGQKQTVSADLATLELSLENHRLQE
                     TEAEKRKQGAERALLEFRESVRTAEQELLLARERREQAQKDTERLQSEIGELEEKEKA
                     LAGEVADLKTRGDYLARTMKKKETDLEGLQTYLETLRGETARHRHQLEGLRQSLLELE
                     ASLGEVKGDLAGRESRRQEWSDRRHHLQQEETKQNQKLQALVTRSEEVKKELAQKREA
                     LERKQKQQAGLEAQVQAAREELVRAEAGFAQLKNEQAVAVSQRDFWRQVVEANEGYPS
                     GVRTLLQKPRPVKGVLDTLANLLTVEARYARAVEAALGEAAGCLVCLNAPAVQKAIAY
                     LNEHGAGRAAFLPLDYFSNGQKPPRYESKKDFSRSDVCGWAVDFVRCAPELRPLAAIL
                     LAGTLVVESGTLLWELVEAEPAPAAGFTGTIVSLDGEIVRSSGLISGGGDRETGLVGR
                     AA"
BASE COUNT         2838 a         3494 c         3732 g         2391 t
ORIGIN      
        1 cggccctttg gaagcgccgc ctttggtgta tgggagcgtc gtctacattc cggtccgggg
       61 tctggggccc ggtctcgtcg cctacgatgt cgtggcgggc agcgagcagt ggcgggcgaa
      121 cctcaaagat gtggccacga cgccgatact ctataaaagc cggctttgcg ccgcttccgc
      181 cgccggggaa gtcaaggggt tggatgtggc caaaggtgag gagctctggc acttcgatgc
      241 cggcggtcca gtgctggcac cgcccatagc ctacgagagt acgacagtca tcggttcggt
      301 gggcggaaaa ctgcacctct tggctatgtc aacgggaaaa gtgatctggg aaaaagagat
      361 gccggaagga gttcgcgcgc aaccggcggt cgtcgacagt accctgtacg tcggcgggtt
      421 ggacggtaat tgttatgccc tggatgtgac gaccggcacg acgcgctggc ggacggccgt
      481 gggcggcaaa atttactcgt cacctgtggt tgccgattcg ctggttttct ttggctgcaa
      541 cgaccggaag gtttacgctc tggcgcaacg ctcaggagcg gtggtctgga cccataccgc
      601 cggaagcatc gtgaacgttc cggtgacacc ggtggggaaa tacctcatct gcggtaccac
      661 ggacggtact ctttttgcgc tccaatccga tgatggtgtc ctggtctggg agtaccagtt
      721 gaaaggcggc ttggctggcc ggccagtggg gagcggtaac cagcttttcg tcaccacgac
      781 cccgggccgg gttttttgct ttaccctccg gtgaccgtta tgacaagcgt agccattttt
      841 cgggaagaag agctccgcac gaatctgcgc gacttagcca ttgaggtcct gcgcgagctc
      901 tggatgtcgt cccggaaggt cggtctctac tccttcgatc atccgggcgt gcagcgggcg
      961 gcggcgaagc cgtttttcta cctcgtccgc tacttcaaat acaagtccgc gttttcctgc
     1021 gtcatccagg accgccggct cttcgtcggc gggacgcccg tgccgccttt ggtttttacc
     1081 aaggggctcg tcgccgagtg ggagcgcgcc gcggtggaca gcgtgctctt ccgcagcgag
     1141 gtgaccgtca atgacctggc ggtcttcctg gaccgcctct ccaagcgctt cccgccgtcc
     1201 gactaccaca atcagtacct tcagtacctg accaagcgca aggtcttttc gattgaaatt
     1261 aacggtgacc tcgccaaaaa gatattctcc cgctcgaacc tctacgatcc ggaactggag
     1321 tacgacttct ccgtggaggg aatggccgcc gaactcctga ggcgcgaagc ggcactggcc
     1381 acagcactct tagccgggga gtggaagtcg gaccgcgagg cgctggagga gggcggcgtg
     1441 gagttccggc tggccgtcct gcgcgaattt gccggcgcag tcatcggcac ctacagcgag
     1501 gaaaaactgc tggcgttggt tgaaagccag atttccggtg aagatttaaa tctcctgatt
     1561 gagaaatcgg agcgggtggc ggcgctcaag aacctcctgc gctcgctggc gcagcacccg
     1621 cagaaagaca aagttttcgg caaggtgaaa gagctcttca ccgcgcgggg cgttcagcgg
     1681 gaaatctacg agcagacatt cgatctggcc gcgctcttcc gcttccgctc ctccgaggag
     1741 accgaagcgc tcatcaacaa agttttctcc agcgcctttg ccccggacga ttttgaacgc
     1801 ttccgcgaag tcttcccccg cctcgtggcc agccgccagg aagaaaaact ccatgaaatt
     1861 ttccgggtgc tcctgctcaa cttaacccgg gaagatcact ccgcccgcca gaaggcgctc
     1921 tttttaatca gacccacctt ggaatctcta gatacgctcc gcgacgaagg aaattttgac
     1981 ttcttaatag atgacctttt gacctttgtc gccgaaaaga aagagacctt tgagttttca
     2041 gaggtgttcg actcctgctt gaaaatctgt ctgaaaaacc ggcgctatca gccggtcgcc
     2101 aaaatggctc tggctctgcg tcagcggctg ggcggccaga accccgatga cgctttcggc
     2161 aatgtcgtgg cccagaaggc gctcgagttt ttcggcgccg atgccgtcgt cgagcaactg
     2221 gtgggagaaa tcgtggaggg gaaaaccacc cagattcagg cgattcgcca ggcgttgacg
     2281 gctatcaata accaaaaggt cgctttggct ctggctgaag ttgtcaccca ccccgaccgg
     2341 gcagtgcggc agaatgccct gaaaatcctt ttgcacctgg gcagtaactc gctggagacc
     2401 ttctcccgga tcttaaacga cggtcagacc ttcatccgtc agcccgacgg caacacgctg
     2461 actcaggcgg cctggtttcg ggtgcggaat gccatccacg ttctgggcaa tctgagggat
     2521 gaacgggcgc tggaggttct gggccgctcg gccattgatc cggatccgcg cgtccggggc
     2581 gaagtggtgc gggcgctgga aaaaatcggc gggactaaag ctggtatcat tctgaagcag
     2641 ttggccagcg accgggacgg cgaagttcgc aaaaccgcca tcatcaccct gggactggtc
     2701 ggtacccccg acgacgtgat tttcctgaga gagctcttcc ttctcggttc gccggagcgg
     2761 ctcccgatct tctatgccgc tggcaacctc ggcggagtgc aggccaaggc cttcttttta
     2821 gaacttttac agcccaattc ggcggcggcc cgtctggcct tcggccgggg ggacgatgtg
     2881 caggtcaagg ctgccattgt caaaggactg gccaaagtca acgacccgga gtgcgccgag
     2941 aaggtccggg aactgggcga ttcgctgaca aatacccaga aagttttcat caaaaatatc
     3001 ctctcttcaa aaacgccgaa aaaaatcctc agccggttga tgcagtagtc gggtccaccc
     3061 tcaagtttta agttccgcca gaacagcagt gacaacggtc ctttcataca tatttacctg
     3121 tcaaaatggc accgtattgg cagttcgtaa taattcaaaa ccttaaatta tttgacaact
     3181 cactgattac caattagtta ctctaatcga aaaacttggc acgagatttg acatacttcc
     3241 ttgtagaaac agacgaggat tgagtgacca tcaggattgt aaccaaaaag ctagatcgaa
     3301 aggaggtgac agatatggcg ttagtcagat ggcgtccagc gcgcgaactc tccttgttcc
     3361 cggaggactt caaccggatg ttcgaagact tctggggccg cttcccgcgt ttctttggaa
     3421 acgatttcgg cgacggcggc acctgggctc cggccgtgga tgtcaccgag accaaggatg
     3481 acgtaatcgt caaagccgag gtccccggta tgaccaagga tgacatcaaa gtcacgcttc
     3541 aggacaacat cctcaccatc cagggcgaaa agaaagaaga gcgcgaagaa aaagaggcgt
     3601 cgttccaccg ggtggagcgg tcgtacggtt ccttcgtgcg ctccttctcg ctacccacca
     3661 cggtgaaatc cgacaagatc cacgccgcct acaaagacgg cgttctgcgg atcaccctgc
     3721 ccaaggtgga agaggtgaag ccgaaggaga tcgcggtttc ggtgaactaa tctggtcacg
     3781 agggcgggtg cgtgggtgga aggaaaaaat tcacccactc ccgcccctcg ggcagattgt
     3841 agggaggtag atgaaaatga aatttatggc attcagagta agtttgctgt ttattggcag
     3901 acatggaacc gcgagggtcg ctgcaattaa ctcacagcag cacgaaagat aataatgacg
     3961 cgcgattggg aagtacaatt cagagagtgg gcgaagccgc tggggcagac cgaacaagac
     4021 cggtgcgaca atgccgtgtc cgcaatccgc aatgcaatca aggcaagcga aaaactccgt
     4081 gcccgtgggg ttttgccctc gtcggtcttt gcccagggct cctatcggaa caacacgaac
     4141 gtccgccaag atagtgacgt tgacatagga atcctttgca cggcctcatt ttgctacgtc
     4201 ctgcccgagc acacgactgc cgagaccttc ggaatcattc ccgcaagtta cctttacggg
     4261 caatacaaga acgaagtcga agaagccctt gtggactatt tcgggcgctc tgctgtcaag
     4321 cgtggcaaca aggctttcga cattagcgaa acgagctacc atgttgaagc cgacgtagcg
     4381 gcgttctttg agcatcgcag ctaccactcg gatggcacat accttaaacc cgaaggtgtt
     4441 gcactccgaa cggacaagga aaaccgccgg gtcataaact ggccagacct gcattacgaa
     4501 aacggggtta acaagaacaa gcagattggc actcggttca agtccttcgt ccggattctc
     4561 aaagctcttt ccatcgaaat gaccgagcag ggcgtccgcg cggggaacat acccggcttc
     4621 ctgctagagt gccttgtctg gaacgtgccg aatgaccgct ttcagcacaa cacctacacc
     4681 gatggcgtga aggcggttct tgtgttcctg tatgagagca cgaagaccga cgaactttgc
     4741 cgggagtggg tggaaatcag cgggctgaag cgtctctttc gtccgacgca gaagtggaca
     4801 cgagagcaag cgaacgcctt cactatggct ggttggaact atgcggggct tggtggacaa
     4861 tcatgaagaa ctttaacaca aagccgttta tttattttct ggctgtgttg tctgtcgtag
     4921 tcttatttgt cgccgcctta attcagggct tcgaccttgg taacttcctc gacgttctta
     4981 gacttatacc tattgttgcc acggtggacg ttatcgccta cttcgtcttc gccggttggc
     5041 tttggcgctg gaagcgtcta cagggctggc tcatcccgtt ccccgacctg aacggcacat
     5101 ggcacggaaa cattcaaacg aactggaaaa atgccgacgg aaacacgccc agacctatcc
     5161 ccgctatctt gactatcaag cagaccttcg ggcgtatgag ttgtgtcatg agaactggcg
     5221 agatggaaag ccacagctac cttgaggggt tctgcatcga caaggacgct caagttcgac
     5281 ggctttgcta ctcctacaca agcagaccga aggccgcttt gcgtgaaagg agcacgccgc
     5341 atgacggcac gatacttttc aacgttgttg gaaatccagt taacaaactc gaaggcgaat
     5401 actggacaca acgtcaaaca gccggaaccg taacactaac ctttcgaacg aaggagcttg
     5461 ttgacgaaat cccggacgac cttccagcgc acccgatcac aggtggcaga ggtcatatag
     5521 taaatgataa gttttgacgt taaaacacag tgcgggaaac cacaaaaaaa catagcggag
     5581 cgcgaatgcg cccgcgataa ggagtgacaa aacatgggta aaatcattgg cattgacttg
     5641 ggaaccacca actcgtgcgt ggcggtgatg gaaggcggcc agccggtcgt cattgccaat
     5701 tcggaaggca tgcgcaccac gccttcggtc gtggctttta ccaaaaccgg agagcgtctc
     5761 gtgggccagg tggccaaacg ccaggccatc accaatcccc tgaacacgat tttctccatc
     5821 aagcggttca tgggacggaa attccgcgag gtgaccgagg aaatcaaaaa cttcccctac
     5881 aaagtggtcg aagggcccaa cggcgacgcc cgggtgcagg tggagaaaga gcaatactct
     5941 ccgcccgaaa tctcggccat ggttttggga aaaatgaagc agaccgccga ggattacctg
     6001 ggtgaaaaaa tcaccgaagc agtaatcacc gtcccggcct atttcaacga cgcccagcgc
     6061 caggcgacca aagacgccgg ccgcatcgcg gggctggacg tcaaacgcat tatcaatgag
     6121 ccgaccgcgg cttcgctcgc ttacggtttg gataaaaaga aaaacgaaaa gatcgccgtc
     6181 tacgacctgg gcggcggcac gttcgacatt tcgattctcg aattgggcga cggcgtcttc
     6241 gaggttcgct cgaccaacgg cgacacccac ttaggcggcg atgactttga caagcggatc
     6301 atcgactggc tcgtggccga attcaaaaag caggagggga tcgatctctc gcgcgacccg
     6361 atggcgctcc agcgtctcaa agaagcggcg gagaagggga agatggaact ctcctccacg
     6421 atggaaaccc agatcaacct ccccttcatc accgccgacc agaccggccc caaacacttg
     6481 aacgtcacgc tcacccgcgc caagatggag cagttggtcg atgatttaat tgagcgcacg
     6541 gtggagccgt gccgcaaggc cttagccgat gccaaactaa ccgttgacca aatcgacgag
     6601 gtcgtgttgg tgggcgggat gacccggatg ccgaaggtcc agcagttagt ccgcgacttc
     6661 ttcaaaaaag agccgcacaa gggagtcaat ccggacgaag tggtggcggt cggcgccggc
     6721 attcagggcg gcgttttagt cggcgatgtc aaggatatcg tcctcttgga cgtgacgccc
     6781 ctttcgctcg gtatcgaaac tttaggcggg gttttcaccc ggctgatcga gcggaacacc
     6841 accatcccga cccgtaaatc ggaaatcttc tccaccgcgg agaacaatca gacgaaggtg
     6901 gaagtccacg tgctccaggg cgaacggcag atggcgatag acaacaaaac catcggccgc
     6961 ttcattctgg acggcattcc gccggcgccg cgcggcatcc cgcagatcga agtcaccttc
     7021 gacatcgacg ccaacggcat cctgcacgtc accgccaaag acaaagccac gggcaaagag
     7081 cagaaaatcc ggatcgaggc ctccagcggt ctcaccgagg cggaaatcaa gaaaatggtc
     7141 acggacgcca aagagcacga aggcgaagat aagaagcgcc gcgaagaagt cgatacccgc
     7201 aaccgggccg accaactggc ctacgaaacc gaaaagaatc tgaaagaatt ggaaggtaaa
     7261 attgaaccgg acgacaaggc caaaatcgaa gcggcggtgg gccgggttaa agaagcgctc
     7321 aaggggaaaa acactgaaga aatccgctcg gcccaggaag ccctcaccca ggtatggcac
     7381 caggcctcgg ccaaaatcta ccagaagacc gccggtcagt ccgccccagg cggatcaacc
     7441 ggtggaacgg aacccccgcc cggcggcgac aaagggccgg agggcggcca acctgtcgat
     7501 gccgactacg aggtcgtgaa gtaagacggc ggctaagtac agatcgctat gccgacgtac
     7561 gaataccgct gcaccgccaa tcaccggttc gaggaggttc agaacatctc cgagccgccg
     7621 attgagaagt gcccccactg cggcgccaaa gcccagcgtg tgatctccgg cggtgccggg
     7681 ctgctcttca agggttccgg tttttacatc accgactacc ggagcgagtc gtacaaaaag
     7741 aaagccaagg aggagtcggg cgaaagtaat ccgcctaagg cggactcagg cggtgagacc
     7801 aagttgtcgg aaacgaagcc ggagaaacca aagaaaacag agggtagtcc ctgaacgccg
     7861 ccggtaaggg aagttactct tctggggccg aaatttcagc aaaccgatgc cgttgccatt
     7921 gatgcgcgtg ccgggcaaat tgtcttcacg tagcattctt tccggggccg cgactttttg
     7981 gctggggatg gccctgacga tttggctcag cgcttgttcc aaaaggcctt ccgaaaagga
     8041 actctttact caggcgcaaa tggcggtaac ggagcagcgc tactacacgg gacttgacct
     8101 ctacgcgcag gtcttgcgga attttccgaa aagcgaaaac gcctacaaag cgctcttcat
     8161 gctggggtat gtcaactccg aattcttgca ccgcccgact gtggcgcgcg actatttcaa
     8221 aaaggtgttg gaaaaatatc ccggctgcga tttggctgac gatgccgttt atttactcga
     8281 caatcagtcc ggtttagccc gaccattgcc gaatcccacc accccctgag ttaacttttt
     8341 gaccggccac aagatatagt ggcgggttca aagatttctc ttgacaaatt tttgccaggc
     8401 ggctatccta tcacctgatc aaggaggtgt gtgcgccttg tgctccagag ggttagccgg
     8461 gccagagtga ccgtcggcgg caatgtgacg ggcgaaatcg gcccgggact gctcatcctc
     8521 ttgggagcca aaagagacga caccgggaaa caagccgact tcctcgcaga caaagttctg
     8581 ggcctgcgga tttttgaaga ccaggcgggg aagatgaatc tttcggttcg ggacgtcaga
     8641 ggagaaattc tggttgtctc gcaatttacg ctgtatgcgg acacccgcag agggagacgg
     8701 cccagtttca ccgaggcgtt ggaaccgcaa caagcggagg aactgtacgg ccattttgtg
     8761 aaccggctca aagccagcgg gctcgttgtc gccaccggct cgttcggcgc gaagatggaa
     8821 gtggaactgg tcaatgccgg gccggtcact ttggtactgg aaaaagagga ggggaaagcg
     8881 gaggcagagg cagagcggtc cgcctgaggc gggcagggaa gttgatgaat tttgtccggc
     8941 cgcaaaccta tacgcgtttt gcggaaaaaa ttaagctcct tctgcgcggc gatcggctca
     9001 ttctggcatc cagttcgccg cggcgagctc agatgttggc tgagttgggc ctttcttttt
     9061 tcgcgctccg cccccggttg gttgaggagg atctggtgat cgcctgtccc aaagcccgct
     9121 cggttgatct ctcgggtcgc aaagccgctt cggtactgcc cgaagcgtcc gccgggttgg
     9181 tactgggcgc ggatacgtcg gtcgtacgcg gaggcgaaat tctagggaaa ccaaaagacc
     9241 accacgaagc gtttgagatg ctgcaccggc tttcgggtca gtggcacgaa gtctacaccg
     9301 gtttaagttt agtcgcggcg ccttcgggcg ccgcctcctc cggctgggaa tgcacccggg
     9361 tgaagttccg ggcgctcacc gcgcgtgcga ttcacgagta catcgcttcc ggagaacctt
     9421 tcgataaagc cggcgcctat ggcatccagg gtctgggcgg ggcgctggtg gagggaattg
     9481 aaggcgacta cgaaaacgtg atcggcctgc ccgtggcgac gttcgctttt ctcctggagc
     9541 agttgcgcct gaccggtccg ccaaatgctt cagttcccgg cgcaccacac ggggagctgc
     9601 ttgccgaccg ggggatacca ggcgaggggt ctggttgacg agcatcggcc agtttttcaa
     9661 agaaaagcgg gaggcgctgg ggctttcgct ggaggagatg gccgagcgga cccggttgca
     9721 ggtgagttac ttagccgctc tggaaggcga ccggctggac gaactgcccg gccggcccta
     9781 ctaccgtttg ttcctgcgga cctacgccgc cagtctgggc ttaaactttg accagattgc
     9841 ccgggaattt ccagagaccg agatcaccgc cgaaatccgt ttgccgcccg cgcaaaccgt
     9901 caagcaacgc gcccggcgag ctggaatcgc agtcgccagc gtggtggtgt tcgcaggcat
     9961 ccttttcttc ttcctgcgct acggccgctt cagcaccacc ggacccggtc aagtggagaa
    10021 atcggctacc gaaactaaat cggccggacc gaaagtggca cctcctcccg cgcgggaatc
    10081 gactgaggtg gcgctccagc cgcctttggt tctccggctg gaaggtctgg aagacaccgg
    10141 cgtgcgggtg gtggctgatc gcgatacgtt gtttaacggg cttttgaaaa aagcggaggt
    10201 cgagcagtgg tttgcgcagg atagtttcat tatcaccctg ggcaaggcct gggccgtgac
    10261 ggcctttgtc aacgagcata agctgaagcg gctgggacgg cccggggagc cgcggtaccg
    10321 attggtcatc cgggcggaca actatgaacg tctggtggac tcaaccgcgc tttcggaacc
    10381 cggacaagag tgagattctt ccagtgtatc tttcgcgcgt agaaattttc ggcttcaagt
    10441 cgttccccca aaggaccgtc ctggccttcg acggcggcat caccgccatc gtcgggccca
    10501 acggctgcgg caaaaccaac attctggacg ccatccgctg gtcgatgggg gagcagcgcc
    10561 ccaccgttct ccgcgggacg aagatggacg aggtgatttt caacgggtcg cggagtctga
    10621 aggctttggg tctggcggaa gtgacccttt cgattcaaaa cgaccgcggc gtattgccct
    10681 ccgaatacga agaagtggcc gtcacgcggc ggcttttgcg caccggcgag agcgaatacc
    10741 tcctgaacca gattccctgc cggctcaagg acatcaccga cctctttctg gacaccggca
    10801 tgggggccca tgcctactcg gtcattcacc aggagatggt tgacctgatc ctctcctcca
    10861 agtccagtga ccgccgggaa ctgttcgagg aagccgcggg agtcgccaag tataagcaaa
    10921 ggcgtactga agccctgcgg aagttggata ctactcaggc cgatctgctg cggctttccg
    10981 atctctccgc cgaagtggaa aagcaggtca cttccctcaa acggcaggta aagaaggcgg
    11041 agcgccacca gcgctacacc gctgaaatca aaacgctgga gctcctgcgc agccggcatg
    11101 agtatttcca gctgcgtcgc cggcgggacg ttgccgccca ggaagcccgg gaactcgaag
    11161 gtcaaaagca aacagtatca gccgatctgg caaccctgga gctttccctg gaaaatcacc
    11221 gcctgcagga gacggaggcg gaaaagcgca agcagggggc cgagcgcgcg ctcctggaat
    11281 tccgtgagag cgtgcggacc gccgaacagg aactcctcct cgcccgggag cgccgggagc
    11341 aggcccaaaa ggatactgaa cgactgcaaa gcgagatcgg tgaacttgag gaaaaggaga
    11401 aggcgctggc cggtgaggtg gccgatctca aaacccgggg cgactacctc gctcgcacga
    11461 tgaaaaagaa agaaaccgac ctggaaggtc tccagacgta cctcgaaacg ttgcgggggg
    11521 agacggcccg ccaccggcac cagctggaag gtttacggca gtcgcttttg gagctggaag
    11581 cctcgctggg tgaggtgaaa ggagacctgg ccgggcggga gagccgccgc caagaatgga
    11641 gtgaccggcg gcaccatctg cagcaagaag aaacgaaaca gaaccaaaag ctgcaggcgc
    11701 tggtcacccg gtcggaggaa gtcaaaaaag agctggcgca gaaacgagag gctctggaga
    11761 gaaaacagaa gcagcaggcc gggcttgagg cgcaggtgca ggccgcgcgg gaagaactgg
    11821 tgcgggccga agcggggttc gcgcagctga aaaacgagca ggcggtggcg gtttcgcaac
    11881 gcgatttctg gcggcaggtg gtggaagcca acgaagggta cccctccggg gtacgcaccc
    11941 ttctgcaaaa gccgcggccg gtgaaagggg tcctggatac tctggccaat ctcctcaccg
    12001 tcgaggcccg gtatgcgcgg gctgtcgaag cggccctggg cgaggccgcg gggtgcctgg
    12061 tctgcctgaa tgccccagcg gtacagaaag ccatcgccta cctcaacgaa cacggggccg
    12121 gacgcgccgc tttcctgccg ctcgactatt tctccaacgg ccaaaagccg cctcggtatg
    12181 agtcgaaaaa agatttctcg cgcagcgatg tctgcggttg ggcggttgat ttcgtccgct
    12241 gtgcgcccga actccggcca cttgccgcca tacttctggc gggaacgctc gtcgtggaaa
    12301 gcggaacgct gctttgggaa ctcgtggaag cggagccggc ccccgcggcc ggcttcaccg
    12361 ggacgatcgt ttctctggat ggcgagatag tacgctcaag cggcctgatc tccggcggcg
    12421 gggaccggga aaccggtttg gtcggccggg cggcc
//
LOCUS       DBNGZD010000146         8788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_441715, whole genome shotgun sequence.
ACCESSION   DBNGZD010000146 DBNGZD010000000
VERSION     DBNGZD010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_441715"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1267)
                     /locus_tag="AC16GM_03990"
     CDS             complement(74..1267)
                     /locus_tag="AC16GM_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922870.1"
                     /translation="MPGLWNNDALTQSLAAIQNGQMVPQFARVAEELQRQKKIDEAVA
                     ICETHLLQFPNYATGHLVLARLYHEKNRLEEARQEYGQVLKLDPENLAAFKGLGDVFL
                     AFGEKGLALWHYRRAAEIDPLSAEVGILVAELSRDEGAWLPAPPTLPGGAARDSRPVQ
                     SQAGSQELPEVDLEVEYTPPPLPKAPEILKAEFASQLGQVKTKEVDADQVLIGFEETG
                     TAPVESNRAELRPGGAPTHTESLAAHETQSLEKPVARPGGAAPVPPPVTPGVGAPLRG
                     ETEAEPTATARAETPPPMDEAEAAIDRAFAGFEEPTGETSHPKTGKVETARTRPAPAR
                     PAGGLAHPLFETESIADIYARQGLGEQALKIYRKLLTQKPDNPVLQEKIKRVESQLSA
                     KTPVK"
     gene            complement(1581..3245)
                     /locus_tag="AC16GM_03995"
     CDS             complement(1581..3245)
                     /locus_tag="AC16GM_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2922871.1"
                     /translation="MNRHVIPTAFVLLLASISSASVAGEHTSPQKIAPALTTRWQTSP
                     DSSVKVWVFFTDKGSADPKVQAQALTNLNQYFSPRALARRQRVNRGQLVFDAADLPVS
                     SNYLSQLSHSGITADQVSRWLNAASVSLTYPQALAAANLPFVREIRPVMRSVQLLPLI
                     EPEVQIPYAPAAPEALNYGNSFRQVHQIKADSLHALGYTGQGILIGMLDTGFFTDHAA
                     FDSLRLQGRLIATRDFINGDFDVQDNAFDGQRNHGTSTFSAVGGFVPGTLVGTAFGAS
                     FALAKTEILATEYVISEEDRWVAGLEWLDSLGADVVSSSLGYNVGDTGFSYTPAQMDG
                     NTAITTQAADLAASRGLLVVNAAGNEGDGPWRIIIAPADGDSVLAVGAVDSLGSRVFF
                     SSTGPTADGRIKPDVMAQGRADWLASAGGGYGRGSGTSFATPLVAGVVALLLDVDSAL
                     TPDQLIQRLHQSGSRASNPDTLMGWGIVNAFAASNLGCARRGDVDNSSVVDIADITAL
                     VQDIVFGTPLPNPVAGDANCDGVRDVIDIVLLIQFVVFGSPTPCCL"
     gene            complement(3424..5700)
                     /locus_tag="AC16GM_04000"
     CDS             complement(3424..5700)
                     /locus_tag="AC16GM_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922872.1"
                     /translation="MRNRWEVSKLKFFGVSVVIALLSFFATYARVNRIQSEGRESGAA
                     TTFTVNLTGDESDSAPGSGGCDIDPITLGFQCTLRAALEEANAHPGVDSINFNVAAVG
                     VPTISPGSALPTITEAVVIDGTTQPTGNRIELNGSGAGFANGLVISAGGSTVKGLVIN
                     RFGNNGIGLVTSGGNVLVSNFIGTDVTGGIALGNGQSGVHINNSPDNIIGGTAAAERN
                     IISGNGMWGVRIQGSSATKNLVQGNFIGTDVRGVARLGNGLDGVIIGFASDNTVGGTT
                     AGTVNIISGNGQSGVFIAGNRATGNVVQGNFIGTDVTGTGALGNSSSGVAFDNAPNNT
                     VGGRTPGARNVISGNNQSGVIIAGSKATGNLVQGNFIGTDVTGAGALGNSGSGVTIDN
                     VSNDTVGGTAAGARNVISGNNQSGVEIGGIVATGNVVLGNCIGTDVTGTAALGNSYAG
                     IFIRETAENNTLGGTVAGARNVISGNERYGVVMAGGATGTIVAGNFIGTDITGTVALG
                     NAENGVSIFGFSSNNTIGGGTEAAGNTIAFNGNEGMVVDLTSRGNSCLHNSIFSNGAR
                     GIDLFPGGVTLNDLGDVDAGSNDLQNFPVFTSAGSVGATVTVQGSLNSRANGTFTLEF
                     FASDSCDPSGYGEGQNFFGSVLVTTDGSGNASFNVTFQVAVAAGKFITATATDSEGNT
                     SEFSQCLTVCALSGDVTNDAVIDIVDLAALVQDIVFGLPLPNPAAGDANCDGVRDIVD
                     VVGLIQYVVFGSPTPCCL"
     gene            complement(5929..8103)
                     /locus_tag="AC16GM_04005"
     CDS             complement(5929..8103)
                     /locus_tag="AC16GM_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922873.1"
                     /translation="MQKAYRFFTVVIFLLASRTPTVYGFDWPELLLYQKNGAAAADQF
                     GYTVAGAGDVNGDGRSDFIVGAPFADPGGLANAGSAFVYSGATGLLLYQKNGPTAGDQ
                     FGQSVAGAGDVDGDGKADFIVGAPFADPGGVGFAGSAFVYSGATGALLFQKNGAAAFD
                     LFGFSVGGVGDATGDNRSEFIVGAHFADPGGLLDAGSAYVYSGATGTLLYQKDGAEGD
                     QFGVSVAGAGDANGDNRADFIIGARLADPTLGLIDAGSAFVYSGATGALLYRKDGAVT
                     GDELGWPVSGAGDVDGDGRGDFLISARLADPGAVVNAGSVFIYSGATGTLLYQKDGAA
                     VEDRFGFWAAGAGDVNGDGKAEFIVGAFKADPGGLADAGSVFIYSGATGGLLFQRNGA
                     AGDSLGFSVAGAGDVNGDGRAEVIVGAPFADPGALSAAGSAFVYALRSVRIASLADVP
                     NDQGKQVIFRWKSFPGNDSLMDLFIVYRRLDSTFNSPGKGVLAADALPLPPGDWLQVA
                     VLAATGDTAYATIVPTLVDSTLAGTKYSVFFVRGRSTNPLLSIDSPLDSGYSIDNLVP
                     APPANLSASGTPGGIVLAWRPVPDFDFDFFFLYRDTVAGFTLSPANRIKATSDTGAVD
                     SPADASKTYYYRVTAVDFSGNEGPPSNEGTAVCAGVRGDVTDDAVADIVDIVALIQEI
                     VFGTPLTNPHAGDTNCDDVRDIVDIVLLIQFVVFGSPTPCCL"
     gene            complement(8239..>8788)
                     /locus_tag="AC16GM_04010"
     CDS             complement(8239..>8788)
                     /locus_tag="AC16GM_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2922874.1"
                     /translation="NDTGDADAGANNLQNFPVLTSALSVGGSTTIEGTLNSTANDSFR
                     LEFFSNDSCDASGYGEGQTFVGSTSVLTDGSGNASINVTLPVAVPGGKFITATATHSA
                     GNTSEFCQCLTVCAGLRGDVTTDGVIDILDLAALVQDIVFGVPLADPAAGDANCDTVR
                     DIVDLALLVQYIVFGSPNPCCL"
BASE COUNT         1819 a         2704 c         2554 g         1711 t
ORIGIN      
        1 gagggtgtcg aggtgtcgga gtgtcggagt gtcggggtta acctcgacac ctcgatacct
       61 cgacaccccg acactacttg accggagtct tggcggagag ttggctttca acgcgtttga
      121 tcttttcctg taaaaccgga ttgtcgggct tctgcgtcag gagtttccgg taaatcttca
      181 gggcttgttc ccctaaaccc tgtcgggcat agatgtcggc tatactttcg gtttcgaaga
      241 gggggtgcgc caaaccgccg gcgggcctgg ccggtgccgg tcgggtccgc gcggtttcca
      301 ccttcccggt cttggggtga ctggtttcac ccgttggttc ctcaaaacca gcgaacgccc
      361 gatcgatggc ggcttccgct tcgtccatcg gcggaggagt ttccgctcgg gcggttgcgg
      421 tcggttcggc ttcagtctca cctcgaagcg gcgcaccgac gccgggtgtg acgggcggag
      481 gaacaggcgc tgctccaccc ggcctggcca cgggcttctc aagagattgg gtttcgtggg
      541 cggccagact ctccgtgtgc gttggtgcgc cgccgggccg gagctccgcg cggttcgatt
      601 cgacgggagc cgtgccggtc tcttcaaaac cgattaagac ttgatcggca tcgacctctt
      661 tggttttcac ctgccccagc tgggaagcga attcggcctt gagaatttcc ggcgctttgg
      721 ggagcggcgg cggcgtgtat tcaacttcca gatccacttc cggcaactct tgagaaccgg
      781 cttggctctg aaccgggcgg ctgtctcgtg cggcaccgcc gggaagggtt ggaggggccg
      841 gaagccatgc gccttcgtcc cgggaaagtt cagccaccag gatccccact tcggcactca
      901 aagggtcaat ttccgccgcc cgccggtaat gccagagcgc cagtcctttc tcaccgaagg
      961 ccaggaagac gtctcccaaa cccttgaacg ccgccagatt ttccgggtca agtttgagca
     1021 cctggccgta ctcctgccgc gcttcttcga ggcgattctt ctcgtggtac agccgcgcta
     1081 agaccagatg ccccgtggcg taattcggga attgcaacag atgtgtttca cagatggcga
     1141 ccgcctcgtc gatttttttc tgccgctgca actcctcggc cacgcgtgca aattgcggca
     1201 ccatctggcc gttctgtatg gccgccagtg actgggtcaa ggcatcgtta ttccacaatc
     1261 cgggcacgcg ctaaaatctc ctctacgttc gactgaaacc tatccgcctg aggcggactg
     1321 tcaagcgaaa tttctctcat cactttaagc aaaacgtgct aaaagggtta gacgtaccac
     1381 gaccctgtgg tcgtctaccc tctcggctta ctacgttaaa atatcggcaa aaaccgggat
     1441 ttgtggaaca tcccgcccgg ggcggggtga gcgggggtca ccccaaatgc tgtgaggtct
     1501 cgtcccctca actattccct ggttttagag acagcacggt gtcgggctgc ggttcggctc
     1561 cgctcaccac acgccccaaa ttacaagcag catggagtgg ggctgccaaa aaccacaaac
     1621 tgaatcaaaa gcacaatatc tatgacgtcc cgcactccgt cgcagttcgc gtcccccgct
     1681 acaggattcg gcaggggcgt gccaaacact atgtcctgca ccagcgccgt gatgtcggca
     1741 atgtcgacga cactgctgtt gtccacgtcc ccgcgccgcg cgcagcccag attggatgct
     1801 gcgaaggcgt tgacgatccc ccaccccatg agcgtatcgg gattggaagc ccggctgccg
     1861 gattggtgca gacgttgaat aagttggtcg ggggtaagag cggaatcaac gtccagaagc
     1921 aacgccacga cgccggccac cagaggagtg gcgaaggaag tgccgctgcc gcgcccgtac
     1981 ccgccccccg cactggccag ccaatcggcc ctcccctgcg ccatcacgtc gggcttgatc
     2041 cgtccgtcag cagtggggcc ggtggaactg aaaaagacgc gagaaccgag cgaatcgacc
     2101 gcgccgactg cgagtacgct gtccccatcc gccggcgcga tgatgatgcg ccagggaccg
     2161 tcaccctcgt tgccggcggc gttgacgacc aaaagcccgc gggaagcggc gagatccgct
     2221 gcctgcgtgg tgatggcggt gttgccgtcc atctgcgctg gcgtgtaact gaatccggtg
     2281 tcgcccacgt tgtagcccaa agaactggaa acgacgtccg ctcccaaact gtccagccac
     2341 tctaaccccg ccacccagcg gtcttcctcg gagatgacgt attcggtcgc cagaatttcg
     2401 gtcttcgcca gggcgaatga cgcgccaaag gcggtgccga ccagcgtccc ggggacaaac
     2461 ccgccgaccg ccgagaaggt ggaggtgccg tggttgcgct ggccgtcgaa ggcgttgtcc
     2521 tgcacgtcga agtcgccgtt gatgaaatcg cgcgtggcga taagccgccc ctgcagccgg
     2581 agactgtcga acgccgcatg gtcggtaaag aagccggtat ccagcatgcc gatgagaatc
     2641 ccctgccccg tgtagcccag cgcgtggagc gaatcggcct tgatctgatg tacctgccgg
     2701 aaggagttgc cgtagttcag cgcttccggc gctgcgggag cgtaggggat ctgcacctcc
     2761 ggttcaatca aaggcaacag ctgcaccgag cgcattaccg gccggatttc ccggacaaaa
     2821 gggagatttg ccgccgccag cgcctgcgga taggtgagcg aaacgctcgc cgcgttgagc
     2881 cagcgcgaga cctgatccgc ggttattccg gaatgggaaa gctgcgagag gtaattagag
     2941 gataccggca gatcggcggc atcgaatacc aattgaccgc gatttactct ttgtctgcgc
     3001 gccaaagcgc ggggagaaaa atactggttt aagttcgtga gggcttgcgc ctgaactttt
     3061 gggtcggcgc tgcccttgtc ggtgaaaaag acccatactt tgacggacga atcaggcgaa
     3121 gtctgccagc gggtagttag tgcgggggca attttctgag gactggtgtg ttctccggcg
     3181 actgaggccg aggaaattga tgccagcaag aggacaaaag cagtcggaat gacgtgccgg
     3241 ttcactaact attattacgg aagatttccg tctaatgcga ggggataata ctttaagaag
     3301 ctattctaat ctccgatccc cgttttgata atgactctcc ctcccctttg atgggagggt
     3361 tggggagggg tcagacaaga tgtctgacct atggaatgaa attggtcttc taatccctgc
     3421 tcatcacaaa cagcaaggcg tagggctgcc gaacaccacg tactgaatca agcccaccac
     3481 gtccacaatg tctcgaaccc catcgcagtt cgcatcaccc gcggcgggat taggtagagg
     3541 caggccgaag acaatgtcct gcacaagcgc ggctaagtcc acgatgtcga tcaccgcgtc
     3601 gttggtcaca tccccgctga gagcgcaaac ggtaaggcac tgcgagaact cagaagtgtt
     3661 gccttcagag tcggtagcgg tggcggtaat gaatttaccg gcggcgactg cgacctggaa
     3721 ggtgacgttg aagctggcgt taccacttcc atcagtggtc accagcacgg agccaaagaa
     3781 attttgcccc tctccgtagc ccgaagggtc acaggagtcg ctggcgaaga attcgagggt
     3841 aaaagtgcca ttcgctcggc tgttgaggct tccttgaacg gtgaccgttg caccgacact
     3901 gccggccgaa gtgaaaactg gaaaattttg caggtcattg gagccggcgt caacatcccc
     3961 cagatcattc aaggttaccc caccggggaa aaggtcaata ccccgagcgc cgtttgaaaa
     4021 aatggaattg tgcaggcaac tgttgccgcg gctggttaaa tctaccacca tgccctcgtt
     4081 accgttgaag gcgatggtgt tgcccgcggc ctccgttcca cccccgatgg tgttgttgct
     4141 ggaaaagcca aaaatcgaca cgccattttc ggcgttgccc agagcaacgg ttcccgtgat
     4201 gtcggtcccg atgaagttac ccgccacgat ggtccccgtt gcacctcctg ccatcacgac
     4261 gccataacgc tcattgcccg agatgacatt gcgcgctccc gccaccgtgc cccccagggt
     4321 gttgttttcg gcggtctccc gtataaaaat gccagcatag gagttgccca gagcggcagt
     4381 gccggtaacg tcggtgccaa tgcagttccc caggaccacg ttcccggttg ccacaatccc
     4441 gcctatttca acgcccgact ggttattacc ggaaatgacg ttgcgcgctc cggcagccgt
     4501 tcccccgaca gtgtcgttgg aaacattatc aatggtcacc ccagagcccg agttgcccag
     4561 agctcccgcg ccagtgacgt cggtgccaat aaagttccct tgcaccagat tcccggtagc
     4621 cttgctcccg gctatgataa cgcctgactg attattgccc gaaatgacgt tgcgcgcccc
     4681 gggagtcctt cccccgacgg tattgttcgg agcattatca aaagccacgc cagagctcga
     4741 attgccgaga gctcccgtgc cagtgacatc ggtgccaatg aagttccctt gcaccacgtt
     4801 cccggttgcc ctgttcccgg ctatgaaaac gcctgactgg ccattgccgg aaatgatgtt
     4861 gaccgttccg gcggtcgttc ccccgacggt gttgtcagac gcgaagccga tgatcacccc
     4921 gtcgaggccg ttgcccaaac gggcaacacc cctaacgtcg gtgccaatga agttgccttg
     4981 cacgaggttt ttcgtggcac tggagccctg aatacgaacc ccccacatgc cattgcctga
     5041 tataatgttg cgctccgcag ccgcagttcc cccgatgatg ttatcgggag aattgttgat
     5101 gtgaactcca gattggccgt tgcccagagc aatgccgccg gtaacgtctg tgccgatgaa
     5161 gttgctcacg agtacgttgc caccactggt caccaaccct attccattat tcccaaaacg
     5221 attaatcacc aaccccttta ccgtactgcc tcccgcgctg attaccaggc cattggcaaa
     5281 tccggcaccg cttccgttca gctctatccg gttacccgtc ggctgcgtgg tgccgtctat
     5341 caccactgct tccgtgatag tcggcagggc actgccgggg ctgatcgttg gcacccccac
     5401 ggcggcgacg ttgaagttga tgctatccac gccggggtgc gcgttggcct cctcaagcgc
     5461 cgcccgcaaa gtgcattgaa aaccgagcgt aatcggatcg atgtcgcagc cgccggaacc
     5521 gggagcggaa tcgctctcgt cccctgtcaa gttgacggtg aaagtggtgg cggctccgga
     5581 ttcccggccc tcgctctgga tccgattcac ccgggcgtag gtggcgaaaa acgataaaag
     5641 ggcgatgacg actgaaacgc cgaagaattt caatttgctc acttcccaac gattgcgcat
     5701 gctacctccc gcgcccgcac gactcttgga gaacgagtcg atcccaccag ctcttgagga
     5761 gaagtgagcg gcggcactgt gccgccccgg actcctttct gctgcggcgg cgagcacccg
     5821 cccttcaccc tcgctggtgg taagataaga ggactaattt acttatcaaa agaaaactat
     5881 gcgcactgcg gttcacacca tctgaggcga gctcgccgtg ccttccactt acaggcagca
     5941 gggagttgga ctcccgaaca ctacgaactg tatcagaagg acgatgtcca ctatgtcccg
     6001 cacatcgtcg cagttggtgt ccccggcgtg aggattggtg aggggcgtgc cgaacacaat
     6061 ctcttgaatc agggcgacaa tatcgacaat gtcggccacc gcatcgtcgg tgacatcccc
     6121 ccgcactcct gcgcagacgg ctgtgccctc gttcgacggc ggcccttcgt tgccggagaa
     6181 atcgaccgcg gtcacccggt agtagtaggt tttggaggca tccgccggag aatcgactgc
     6241 gcccgtgtcg ctcgtggcct taatgcggtt ggccggtgaa agggtaaaac cggccaccgt
     6301 gtcgcggtag aggaagaaga agtcaaagtc gaaatccgga actggacgcc aagccaggac
     6361 gatgccgccc ggtgtgccgg aggcggagag attggccggc ggcgccggga cgaggttgtc
     6421 aattgagtag ccggagtcca aaggcgagtc aatcgacagg agaggattag ttgagcggcc
     6481 gcgcacaaag aagaccgaat actttgtccc ggccagggtg gaatcaacca gcgtcggtac
     6541 gatagtcgca taggccgtat caccggtggc cgccagaacc gctacctgca gccagtcgcc
     6601 cggcggcagg ggaagggcat cagccgccag cacacctttt ccgggcgagt tgaacgtcga
     6661 atcgaggcgg cgataaacga taaataagtc cataagcgag tcgtttccgg gaaaactttt
     6721 ccagcgaaaa atcacctgtt tcccctggtc gttggggaca tcggccaggg aggcgatccg
     6781 caccgaacga agtgcataca cgaaagccga accagccgcc gagagtgcgc cgggatcggc
     6841 aaagggggcg ccgacgatga cctcggccct gccgtcaccg ttcacgtccc cggcgccggc
     6901 gaccgagaag cccagggagt cgccagccgc accgttcctt tggaagagca gtccgccggt
     6961 cgccccggaa tagatgaaaa ccgatccggc gtcggcgagc cctccgggat cggctttgaa
     7021 agcgccgacg atgaactccg ctttcccgtc accattgacg tcacccgcac ccgccgccca
     7081 gaagccgaac cggtcttcaa cggcagcgcc gtctttctga tagagcaatg ttcccgttgc
     7141 cccggagtaa ataaagaccg aaccggcatt gactaccgca ccgggatcgg ccagccgcgc
     7201 gctgatcaga aaatcgccgc gcccgtcgcc atcgacgtct cccgcacccg atacgggcca
     7261 gcccagttca tcgccggtga cggcaccgtc tttccggtag agaagcgccc cggtcgctcc
     7321 ggagtaaacg aaggccgacc cggcatcaat gagcccgagg gtcggatcgg ccaggcgcgc
     7381 gccgatgatg aaatcggcgc ggttgtcgcc attcgcgtct cccgcaccgg ctaccgaaac
     7441 gccgaattga tccccctccg caccgtcctt ctggtacagg agtgtcccgg tagcaccgga
     7501 gtagacgtag gccgagccgg catcgaggag gccgcccgga tcggcgaagt gcgcgccgac
     7561 gatgaattca gaccggttgt cgcctgtggc atctccgact cccccgacag aaaaaccaaa
     7621 gagatcgaac gccgccgccc cattcttctg gaaaagcagc gcccccgtcg cgccggagta
     7681 aacgaaggcc gaaccggcga acccgactcc gcccggatcg gcaaagggag cgccaacgat
     7741 gaaatcggct ttcccgtcgc cgtccacgtc acccgctccg gcgaccgact ggccgaactg
     7801 atcacccgcc gtggggccgt ttttctggta caagagcaaa ccggtggcac cggagtaaac
     7861 gaaagccgac ccggcattgg cgagcccgcc cggatcggca aagggggcgc cgacgatgaa
     7921 atcacttctc ccgtcgccgt tgacatcgcc cgctccggcc acggtatagc cgaactggtc
     7981 agcggccgca gctccgtttt tctggtagag cagaagctcc ggccagtcga acccgtagac
     8041 ggtcggcgtt cgagaggcca acaaaaaaat cacgactgtg aagaaccggt acgctttctg
     8101 catatagtta ctcaaaagcg cgagtcccct ccctgttcct cgctggtgtt taatataatg
     8161 ccccctggag gttgtcaagc aattttgtct gtttgtggct taactaatcc ctaattcctg
     8221 gtctctaatc cctgcctttt agaggcagca cgggttcgga ctcccgaaca cgatatactg
     8281 caccagaagc gccagatcga ctatgtcccg caccgtgtcg cagttcgcat ccccggcggc
     8341 gggatcggcc aaaggcacgc cgaacacgat gtcctgcacc agcgcggcca ggtccaggat
     8401 gtctatgacg ccatctgtgg tcacatcccc gcgcagtccg gcgcagacgg tgaggcactg
     8461 gcagaactcc gaagtgttcc ccgccgaatg ggtcgccgtg gccgttatga atttaccgcc
     8521 gggaacggca actggaagag taacatttat gctggcgttg ccgctgccat cggtcaaaac
     8581 gctcgtcgag ccaacaaaag tctgcccttc cccatagccg gaagcgtcgc aggaatcatt
     8641 ggagaaaaac tccagccgga aactatcgtt ggccgtactg tttagcgtcc cctcaatggt
     8701 agtgctacct cctacgctta aggctgaagt caaaaccgga aagttctgca gattgttcgc
     8761 tccggcgtcg gcatccccgg tgtcattc
//
LOCUS       DBNGZD010000147        13857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1205880, whole genome shotgun sequence.
ACCESSION   DBNGZD010000147 DBNGZD010000000
VERSION     DBNGZD010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1205880"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..2130
                     /locus_tag="AC16GM_04015"
     CDS             88..2130
                     /locus_tag="AC16GM_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922875.1"
                     /translation="MKTSRKIETSLFLLLVLLAGSGCVYYNTFYYARRAYNKAYQEQK
                     KQGQLKANPAQSRQYEEAIQRASKVLANHPRSSWVDDALLLIGKSYFYEGEYQKAERT
                     FRELIAGFPKSKFVPEAQFFIGWSLVLSGSYDNAMTELQRTLGLTKNKDLLGRVEMQL
                     AETFSIQEEYDSAIVHFQTLVERYGNDKEKGMAQFRTGESFMKMDNHREAAAAFEKVL
                     KYSPPLEQYFNARFYQAGCLCESKELNQGIAILRGLARDERFYKNSGRVNLKLAEAHL
                     LLDSVSTAIDLCEKVTSLFPQTPDAAQAYYQLGEIYQKRLGDLKKAKEQFDKAASEGR
                     GSEYGRLAVARSSDIAKLETYQKAISSEETEKAAQSQFLLGEVYYHQLDQPDSAVREY
                     RSVVENHPESPYAAKALAALAYIYLEEHQDTTQARAAYRALLDNYPHADCARDAIYFL
                     GLAGTAADTGYPAKVFADGERLLANDHNVDSALTLFRSIPVRFPQSQYVPRAFFTVAW
                     ILENFKAPADSSVYYAYRLVADSFPNSPYAQQALVKLGSPPRRTAPPPPPVVKPDTTV
                     IPKDTLALDTTLVTRAAIPMAPRPIHDPQFVYPPSQKDSGFQDRLRFKILINFLGNVA
                     DVELLNPSSIQEIDEAAKLAVHNLQFDPLKINPRQYNTWFIYELDVIPPAQAPFHP"
     gene            2225..3076
                     /locus_tag="AC16GM_04020"
     CDS             2225..3076
                     /locus_tag="AC16GM_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR2922876.1"
                     /translation="MKAKLTGEVTAVRFFSPAIVRLDFQAPHITAHCRPGQFIALKAS
                     DRYDPLLRRPMSLFTVDPRKKTAAILFKIFGPGTRYLANRKKGQTLDFLGPLGNRFVI
                     PARTDRVIIVAGGLGLAPLNFLLEELIRKRKHPQDRLLLLLGARVKEDLVALRKSLRL
                     PTKTALATDDGSVGFRGTVTQLLEKELDGKKNDHRHTVLFACGPEGMLQATCRIANER
                     QIFGQIAVENHMACSFGICMGCMVRCRKKVTPPSEETDRTEISREVYQRACTEGPIFT
                     MHEVVWP"
     gene            3073..4011
                     /locus_tag="AC16GM_04025"
     CDS             3073..4011
                     /locus_tag="AC16GM_04025"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR2922877.1"
                     /translation="MKGGKRYGKPDLSVTIAGIPFANPVWVASGTVGYGEELAEIIDL
                     SKIGAIVTKTITLEEREGHPYSRTVETAGGMLNAIGLQNVGVERFIKEKLPFLAQIKK
                     TRIVVNIGGSSIDDFVAVAQRLSDHGPIDMLEANISCPNVKEGGHEFSATPQPAFQVI
                     AAVVKASRFPVIAKLSPNTANVAAVARAVAEAGAAAISLINTLVGLAIDIDARRPVLS
                     NVTGGLSGPAIKPVALAMTLQVARAVKLPIIGMGGIMTVEDTVQFFLAGATAIQIGTA
                     NFVDPTTSLTIVQGLEKYLRQKKISRITDLVGKLET"
     gene            4013..4855
                     /gene="pyrF"
                     /locus_tag="AC16GM_04030"
     CDS             4013..4855
                     /gene="pyrF"
                     /locus_tag="AC16GM_04030"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR2922878.1"
                     /translation="MKNTTPFREKLTRAIRNAQSFLCVGLDPEPEKLPAACQKAADPV
                     LEFNRRIIEATTDIVCAYKPNLAFYEALGKSSWETLNETCKAIPKNLPIILDAKRGDI
                     GHTARLYAQALFDQLGGDAVTVNPYLGGDALVPFADYVDRGIFVLCLTSNPGAGDFQT
                     RLVNGHPLFLEVVRKVVEWDRHRNFGLVIGATYPDQLQQVREAAGDLPILIPGVGAQG
                     GDLEKSVQYGTRNGTNLAIINVSRAILYASSGSDFAEKARAKALELKTAMLSAVGAKI
                     TLSY"
     gene            4883..6397
                     /locus_tag="AC16GM_04035"
     CDS             4883..6397
                     /locus_tag="AC16GM_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR2922879.1"
                     /translation="MRAIDRRAIEDLGLPILDLMEKAGLGVALAARQMLGGELEGKTI
                     AVLCGKGNNGGDGLVAARYLAQGGGKVTIYLLARATELAGAAKTNLTRAQEAKIRIVE
                     LSDAKQLPETCDLFIDAIVGTGFQGTLTSLAAEVLQKVSSSERAVLAVDIPSGVDATT
                     GEIKGPVAKARKTVTFAWPKVGQFLFPGKFYSGEVQVVDIGIPKSGAEPEKVNLHLLT
                     KSEIAQNLPLRKPDGHKGTFGKVLVIAGSEGLTGAACLASLAALRSGCGIVILGCPES
                     LNDIFEIKLTEVMSRPLPEVKHRRVLSLRSLGEILKLMEWADVVALGPGVGTHHETQD
                     LVRRLLMRIEKPVVLDADGINALAKDNSALKNRTHPTIVTPHVGELARLLSESPEELN
                     RNRIESARQAARELNCIVVFKGAPTLIGEPSGNVYLNPTGNSGMATAGSGDVLTGAIA
                     GIWAQGAKPVQAACAGVFLHGLAGDLSRDAKNEYSLIAGDILDHLPQTISSLFS"
     gene            6462..7748
                     /locus_tag="AC16GM_04040"
     CDS             6462..7748
                     /locus_tag="AC16GM_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HIR2922880.1"
                     /translation="MRVQPLKFVVPVLLALVVLLSLCEPASAQKLLIPMDLAQADHLK
                     AYGIAYWCLQNAHNVEWLLNYRGGSFLTDGWQALADLCRLRGVTFTPIDAAGAGQIYQ
                     TIEQGNMDVVLLEKSPKLAVYVPANREPWDDAVRLALDYAEIPYTTLWDTEVLRGDLA
                     KYDWLHLHHEDFTGQYGKFYGAYRNALWYREEVATNEALAHALGFRKVSEEKKAVART
                     IKDYVTSGGFVFAMCSAPETMEIALAAEKDDIVAQEFDGDPPDPDYQKKLDYSQCLAF
                     ENFVVYPDALDNRRSNIDTITDRLRYIKNQETDYFTLFDFSAKLDPVPTMLVQDHVNA
                     VAGFMGLTTAFRKSLLKKYVTILAEVEGEDEVRYIHGNYGRGTFTFYSGHDPEDYQHF
                     INDPPTDLSLHKNSPGYRLILNNILFPAARKKERKT"
     gene            complement(7896..8501)
                     /locus_tag="AC16GM_04045"
     CDS             complement(7896..8501)
                     /locus_tag="AC16GM_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2922881.1"
                     /translation="MTSQRKVKVLVVTADQPQAAELGQALVQKGYQAQVTGKAATALA
                     KIHSENPHIVCIDQTLPDKSGLDLLREIRKHDTDLCVIMLTDQPTVEQAVASFKLGVF
                     DYLKKPSKPADLWPVVERAVVERGLVTDLAERVNLKIGERLRALRLKKDLTLQQVANR
                     ADVSVSLISQIERAQSSASLETLTKLASALGAKLASLFEGL"
     gene            complement(8576..8932)
                     /locus_tag="AC16GM_04050"
     CDS             complement(8576..8932)
                     /locus_tag="AC16GM_04050"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR2922882.1"
                     /translation="MTSDLQYVRVAALADISEGEGKRVRVGLAVIVLFKISGDLFAVE
                     GLCRHMKAPLEFAQVQPNQTITCSWHGWQYDLTSGQCLTTSWAKLKTYPVKIENGQVW
                     VSVPGPVEPEKSSAAL"
     gene            9043..9444
                     /locus_tag="AC16GM_04055"
     CDS             9043..9444
                     /locus_tag="AC16GM_04055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIR2922883.1"
                     /translation="MLQVAAAVIVQNGKILIGQRKKTDRFPLKWEFPGGKVQSKESPE
                     TGLLRELSEELGIEAQIGNKLTEVTYAYPNFGPVRIQFFTVTRYHGTLENRAYEQIAW
                     VNPAQLPDYDFLEADRSLIRELARSTVAGGR"
     gene            9582..10364
                     /locus_tag="AC16GM_04060"
     CDS             9582..10364
                     /locus_tag="AC16GM_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD family protein"
                     /protein_id="HIR2922884.1"
                     /translation="MMLQQTQVERVLPKYREFLKKFPTLTSLAKAPVAAVIRCWCGLG
                     YNQRAVYLHRAARLLIERNGGKFPRVPVELQKFPGVGPYTAGAVAIFACNRDVVMLDT
                     NIRRVLTRVFAGQRPVSEKKLHKLAFRLLPAGRSKIWHSALMDLGALLCTTRNPKCPL
                     CSVQRDCLWYRESQRKPRPASRPARRKQPPFRNSTRFWRGRIVAALRELSNGRKFSLF
                     QLGKRIKPNFKTADLPWLKRLATALVRDGLATQSGRQISLPT"
     gene            complement(10501..11355)
                     /locus_tag="AC16GM_04065"
     CDS             complement(10501..11355)
                     /locus_tag="AC16GM_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR2922885.1"
                     /translation="MKISQIFGLSVCFLPASAGCFTSAQAEPADSVHANQTAGAAQNS
                     TLKALDHLVSEFWTDTKSVVFAPTHWQKKDWVVCASAGAVTVGLLATDHDVEAEISRR
                     RSNLSDDLARWVTPLGSREGASLAMGGLYVGGIVFRHPTSRRAAWNSFKAVAIAQGIT
                     QVLKHGLNRRRPGALQNPGHFDGPTWHSPETGLSFPSGHTTTAFALASAISRTYRKPY
                     VTIPAFTLAGTVALARLNDFVHYPADVFFAAAMATWVGQVVSREKGERKVQVGLLSGN
                     RAGLVVRF"
     gene            complement(11387..12133)
                     /locus_tag="AC16GM_04070"
     CDS             complement(11387..12133)
                     /locus_tag="AC16GM_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922886.1"
                     /translation="MPLFPRRSVRTYSGVVLFFLLLTPTVAFAHREDYLNKTFVYQTL
                     DHRELELEYWAHYGSTSVSRSVLNTWQHTASFEYGILEHWMVEAFSTFQHLPGRNLGY
                     VATHVETRVRLFDEGKKFLDPAFSLEYSHSNAEGVSIHSLEPTIVLSKDLQRLNFTLD
                     FSLEQQLNAGHETEFGYAIATRYDTKHLLRIGAEAQGVFGHAAPHYLVPQVHLVLPAE
                     VTAKVGVGFRFSGRGEKIFLQLLTELGLEL"
     gene            complement(12180..12563)
                     /locus_tag="AC16GM_04075"
     CDS             complement(12180..12563)
                     /locus_tag="AC16GM_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2922887.1"
                     /translation="MKSVGAVLIYVKEIERVKAFLRDSLGLHLFDSKGDWAGFKLGEV
                     LIELFGPQKSGLDVGVPGRSGIKDREQQPLRLSFKVDDIEAEVARLKSKGVRLVTPVG
                     EESWGKYVWFADPEGNQYQLWKPAK"
     gene            complement(12601..13500)
                     /locus_tag="AC16GM_04080"
     CDS             complement(12601..13500)
                     /locus_tag="AC16GM_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIR2922888.1"
                     /translation="MNILVAGGTGFVGSEIARQALARGHTMRCLARGTRDVSGTVREL
                     KRLGAEICHGNLAEPASLTSAFAGVNVLVHAIGIIRERKEQTFEKLVVTGTRNLVAEA
                     KRAGVRQIIYISAAGTRENARSTYHRTKWQAEQIIIQSGIPYTVFRPSVIFGPRDAFI
                     NLLAKLIRYNPVVPVIGSGLSKLQPVSVKDVAACAVKSLADPRAQNQIFEIGGPQAYT
                     YNELLDVVAKVMLKKRMKIHLPLALMRPVVRLLEIMLTNPPVTSEQLIMLQEDNVCDN
                     RKLIGTFGIALLPLEAGIREYLT"
     gene            13698..>13857
                     /locus_tag="AC16GM_04085"
     CDS             13698..>13857
                     /locus_tag="AC16GM_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HIR2922889.1"
                     /translation="MERTTTISGTGHAVPENVLTNQDLEKMVDTSDEWIIERTGIRKR
                     RIGNATTTA"
BASE COUNT         3145 a         4104 c         3704 g         2904 t
ORIGIN      
        1 cctgtttgcc gactatctca atatcctgac cgagctgtgc gggaaggttg ataccatggg
       61 acacgtgaag accagcgggc atgaactttg aaaacttctc ggaaaattga gacttctctt
      121 tttctcctgc tcgtcttgct ggcaggcagc ggttgtgtct actataacac gttctattat
      181 gcccgacgtg cgtataacaa ggcctaccag gagcagaaaa agcaggggca gctcaaagcc
      241 aacccggccc agtcccggca gtacgaggag gccatccagc gtgcttccaa agtgctggcc
      301 aaccaccccc gctcctcgtg ggtggacgat gccttattat tgatcgggaa gagttacttc
      361 tacgaaggcg agtaccagaa ggccgagcgc accttccgtg aattgatcgc aggcttcccc
      421 aagtccaaat tcgtgccgga agcccagttt ttcatcggct ggtctttagt cctgtcggga
      481 agctacgaca acgccatgac cgaattgcaa cgcaccctgg gcctcacgaa aaacaaagac
      541 ctcttgggtc gggtcgaaat gcagttggcc gaaaccttct ccatccagga agaatatgac
      601 agcgccatcg tccacttcca gacactcgtg gagcggtacg ggaatgataa ggaaaagggg
      661 atggcccagt tccggacggg ggagtccttt atgaaaatgg acaatcaccg ggaagccgcc
      721 gccgcttttg agaaagttct gaaatacagt ccgccgttgg aacagtactt caacgcccgc
      781 ttctaccagg ccggctgcct ctgcgagagc aaggaactga accagggtat cgccatcctg
      841 cggggcctag ccagagacga gcgcttctat aaaaattcag gtcgggtgaa cctgaaactg
      901 gccgaagccc acctcctttt ggactcggtc tccaccgcca tcgacttgtg tgaaaaagta
      961 acttccctct tcccgcaaac tcccgacgcg gcccaggcct actaccagtt aggcgagatt
     1021 tatcaaaaac ggctcggcga tttgaaaaaa gccaaagaac agttcgacaa agccgccagc
     1081 gaaggtcgcg gctcggaata cggccggctg gcggtcgcgc gctccagcga catcgccaaa
     1141 ctcgaaacct accaaaaagc gatctcctcc gaagaaacgg aaaaggccgc tcaatcgcag
     1201 ttcttgctcg gcgaagtcta ctaccaccag ttggatcagc ccgattcggc ggtgcgggag
     1261 taccggagtg tggtcgaaaa ccacccggaa agcccctacg ccgccaaagc gttggccgcg
     1321 ctggcctaca tttacctgga ggagcatcag gacacaaccc aggcgcgggc cgcctaccgg
     1381 gcactcctgg ataactaccc tcacgccgac tgcgcccggg atgccatcta ctttttggga
     1441 ttggccggta cggcggcgga caccggttat cctgcgaaag tatttgccga cggtgagcgg
     1501 ctcttagcca acgaccacaa cgtcgattcg gcgctcaccc tttttcggag tatccccgtg
     1561 cgtttccccc agtcccagta cgtgccgcgg gccttcttca ccgtcgcctg gatactggaa
     1621 aatttcaaag cccccgcgga ctcgagcgtc tactacgcct accgcctcgt agccgacagc
     1681 ttcccgaatt ctccctacgc ccaacaggcc ttggtcaaac tggggagtcc tccccgccgc
     1741 acggctcctc cgccgccacc ggtagtcaaa ccggatacca ccgtcattcc caaagatacg
     1801 ctcgccctcg atactactct ggtcacccgg gccgccatcc ccatggcccc gcggcccatt
     1861 catgatccgc agttcgtcta tcccccttcc caaaaggatt ccggcttcca agacagactt
     1921 cgcttcaaaa tattgatcaa ttttctgggt aacgtagccg atgtggaact cctgaatcct
     1981 tcaagcattc aagagatcga tgaagccgcc aaactggcgg tgcataatct tcaatttgac
     2041 cccctgaaga ttaatccaag acagtacaac acctggttca tctacgaact ggacgtcatt
     2101 ccaccagcac aggctccgtt ccacccctag taaacatctt ttccgtctct cggatcgtct
     2161 cagtaaatgc ttggttttct ccggcttttt cttctgcgga aaaaatgtta taatgaatta
     2221 tctgatgaaa gctaaactga ccggggaggt caccgccgtc cgttttttct ctcccgcgat
     2281 cgtacgtctt gacttccaag ccccgcatat tacggctcat tgccgccccg gccagtttat
     2341 cgctctcaaa gccagtgatc gctacgatcc gctcctgcgc cgcccgatgt cgcttttcac
     2401 cgtcgacccc cgcaaaaaaa cggctgccat tctgttcaaa attttcggcc ccggaacgcg
     2461 ctacctggcc aatcgaaaaa aaggacagac gctcgacttt ttaggccctc tggggaatcg
     2521 cttcgtcatc cccgcccgca ccgaccgggt catcattgtc gctggcggcc tcggcctggc
     2581 accgctgaac ttcctgttag aagagctgat ccggaagcga aagcatccgc aggatcgtct
     2641 tttgctgctc ctcggcgccc gggtaaaaga agacctggta gcgctgcgga agtcgttgcg
     2701 gctgccgacg aagaccgccc tggccaccga tgacggctcc gtcggattcc ggggaacggt
     2761 cacccagctt ttggaaaaag aacttgacgg aaaaaaaaac gatcaccgcc acaccgtcct
     2821 tttcgcctgc ggccccgagg ggatgctcca ggccacctgc cggattgcca atgagaggca
     2881 gattttcggc cagattgccg tggagaacca tatggcctgc agtttcggca tctgtatggg
     2941 gtgcatggtg cgctgccgca aaaaggtgac tcctccgtcg gaggaaacgg accgaactga
     3001 aatttcccgc gaagtctacc agcgtgcctg caccgaaggc ccgatcttca ccatgcatga
     3061 ggtcgtctgg ccgtgaaggg ggggaaaaga tacggaaaac cggatctctc cgtgacgatt
     3121 gccgggattc ctttcgccaa tccggtctgg gtcgcctccg gcacggtggg ctacggtgag
     3181 gaactggctg aaataatcga cctctctaaa atcggggcca ttgtcaccaa aaccatcacc
     3241 ctggaagaac gggaaggcca tccctactcg cgcacggtgg aaaccgcggg cgggatgctg
     3301 aatgccatcg gcctgcagaa tgtcggcgtg gagcgtttca tcaaagaaaa actacccttc
     3361 ctggcgcaaa ttaagaaaac ccgaattgtg gtgaacatcg gcggctccag cattgatgac
     3421 ttcgtggcag tggcccagcg tctttccgac cacggcccga tcgacatgct ggaagccaac
     3481 atttcctgcc cgaatgtgaa agaaggcgga cacgaatttt cggccacccc ccagcccgct
     3541 ttccaggtca ttgccgctgt cgtcaaagcc agccgctttc cggtgattgc caaactctcg
     3601 cccaacaccg ccaacgtcgc tgccgtcgcc cgcgccgtgg cggaggcggg cgccgccgcg
     3661 atctcactca tcaacaccct cgtgggtctg gcaattgaca tcgacgcccg gcgaccagtc
     3721 ctttcgaacg tcaccggcgg cctctccggc ccggctatta aaccggtggc cctggcgatg
     3781 accttgcagg tggcacgggc ggtcaaactc cccatcatcg gcatgggggg gattatgacg
     3841 gtggaagata cggtgcagtt cttcctggcc ggagccaccg ccattcagat cggcactgcc
     3901 aatttcgtcg atcccaccac cagtctcacc atcgtccagg gtctggaaaa gtatctgcgc
     3961 cagaagaaaa tttcccgcat caccgacctg gtcggcaaat tggaaacgta gggtgaagaa
     4021 caccactcca tttcgggaaa aactcaccag ggccatccgg aacgcacaat cattcctctg
     4081 tgtgggactt gatccggagc cggaaaaact gccggcggcc tgccaaaagg cggctgaccc
     4141 ggtactggaa ttcaatcgcc ggattatcga agcgaccaca gacatcgtct gcgcctacaa
     4201 gccgaacctc gccttctacg aggctctggg gaaaagcagc tgggaaacgc tgaacgagac
     4261 ctgtaaggca attcctaaaa acctgcccat tattctggat gccaagcgcg gcgatatcgg
     4321 gcataccgcc cggctctatg cgcaggcgct ttttgaccag ttaggcggcg atgccgtgac
     4381 ggtgaatccc tatctgggag gagacgctct agttcctttt gccgactatg tggatagagg
     4441 gatcttcgtg ctctgtttga cctccaatcc gggagccggt gattttcaga cgcggttggt
     4501 aaacggtcat ccgctcttcc tggaggttgt ccggaaggtg gtagagtggg atagacacag
     4561 gaattttggt ctggtcatcg gcgccaccta tcccgatcaa ttgcaacagg tccgcgaggc
     4621 ggccggagac ttgcccatct tgattcccgg cgtgggcgct cagggcggcg atctggaaaa
     4681 gtcggtgcag tatggaacga ggaacggaac caatctggcc atcatcaatg tctcgcgagc
     4741 cattctgtac gcatccagcg gaagcgactt cgccgaaaaa gcgcgcgcga aggcactgga
     4801 attgaaaacg gccatgcttt ccgccgtggg cgcaaagatt actctgagtt actaaccgat
     4861 gtaccttttg agtgctgaac agatgcgggc catcgaccgc cgggcgattg aagacctcgg
     4921 ccttccgatt ctcgatttga tggaaaaagc cggtctgggc gtggcgctgg cagcgcggca
     4981 gatgttgggt ggcgaactgg aaggcaaaac aattgccgtc ctctgcggca aagggaataa
     5041 tggcggcgat ggtctagtag cagcacgtta tttggcccag gggggtggga aagtaacgat
     5101 ttatctctta gccagagcaa ctgaattagc cggtgccgcg aaaacgaatt tgactcgcgc
     5161 tcaggaagcg aagattcgga tcgttgaact atcggacgca aaacaactgc ctgaaacttg
     5221 cgacctgttc atcgatgcca tcgtcggcac gggttttcag ggaaccctca ccagtctggc
     5281 ggcggaggtc ttgcagaaag tgtcctcaag cgagcgggcc gtgctggcag tcgatatccc
     5341 ttcgggtgtg gacgcgacca ccggcgaaat caaagggcca gtggcaaaag cgcggaagac
     5401 cgtgactttt gcgtggccaa aggtggggca gtttctcttt ccggggaaat tctattcggg
     5461 cgaggtgcaa gtggtagaca ttggcatccc gaagagcggt gctgagccgg aaaaggtcaa
     5521 cctgcatctt ctgaccaaat cagaaatcgc acaaaacctc cccctccgaa agcccgacgg
     5581 ccacaaaggc actttcggca aagtattagt catcgcgggc tcggaaggtc tcaccggcgc
     5641 cgcctgcctg gcctctttag ccgcgctccg cagcggctgc gggatagtca ttttaggttg
     5701 tcctgaaagt ttgaacgaca ttttcgaaat caaactgacc gaagtgatga gccggcccct
     5761 gcctgaagtg aagcacagac gcgttctctc cctgcggagt ttaggcgaaa ttttgaagtt
     5821 aatggaatgg gcggatgtcg ttgccctggg ccccggggtg ggaacgcacc atgagacgca
     5881 agacctggtc cggcggctcc tgatgcgtat tgaaaaaccg gttgtgctgg atgccgatgg
     5941 gattaatgcg ctcgccaaag ataactcggc tctgaaaaat cgcacacatc cgaccatcgt
     6001 caccccccac gtgggggaac tggcccgcct cttgagtgaa tcgccggagg aattgaaccg
     6061 caatcgaatc gaaagcgcgc gccaggccgc ccgcgaatta aattgcattg tcgtcttcaa
     6121 aggtgcgccg actttaatcg gggaaccctc tggaaatgtc tacctgaacc ccaccggcaa
     6181 ctccggcatg gctaccgccg ggtcaggtga cgtgctgacg ggagccatcg ccggcatttg
     6241 ggcgcaggga gccaaaccgg ttcaagcggc ctgtgccggt gtgttcctcc acggcttagc
     6301 cggcgatctg agccgtgatg caaaaaacga gtacagtctc attgcggggg atattctcga
     6361 ccacctgccc cagacgattt cgtcactttt ctcttgaatt ttccagtacg actccggtaa
     6421 aattctttca gaaagactga attctgccgt ataaccaaaa gatgcgcgtg cagccattaa
     6481 aattcgtagt gcctgtactt ttggctctcg ttgttctcct ctccctgtgc gaacccgcct
     6541 cagcccagaa actcttaatc ccgatggacc tcgcacaggc cgaccacctg aaggcctacg
     6601 gcattgccta ctggtgttta cagaacgccc acaatgtcga gtggctattg aattaccgcg
     6661 gcggttcgtt tttgaccgac ggctggcagg cgttagcaga cctctgccgc ctgcgggggg
     6721 tcacttttac acccatcgac gccgccggag ccggacagat ttaccagacc attgaacagg
     6781 gcaacatgga cgtggtgctg ttggaaaaat cacccaaatt ggccgtctac gtgcctgcca
     6841 accgcgagcc ctgggacgac gcggtacgcc tagctttgga ctatgccgaa attccgtaca
     6901 ccaccttgtg ggacaccgaa gttctacgcg gcgacctggc caagtacgac tggctccacc
     6961 tgcaccacga ggacttcacc ggccagtacg ggaagttcta cggcgcttac cggaacgcgc
     7021 tctggtaccg cgaggaagtg gccacgaacg aagcgttggc ccatgctttg ggcttccgca
     7081 aggtttccga agagaaaaaa gcggtcgccc ggaccatcaa ggattacgtc acctccggcg
     7141 gtttcgtctt cgcgatgtgc tccgcgccgg aaaccatgga gatcgccctg gccgccgaaa
     7201 aggacgacat tgttgcccaa gagttcgacg gcgacccgcc cgacccggat taccagaaaa
     7261 aactcgacta ctcccagtgc ctggcttttg agaacttcgt ggtctacccc gatgccttgg
     7321 acaaccgccg ttccaacatc gataccatca ccgaccgcct gcgctacatc aaaaaccagg
     7381 aaaccgatta cttcaccctg ttcgactttt cagccaaact cgacccggtg ccgacgatgt
     7441 tagtccagga ccacgtcaac gcggtggccg gatttatggg attgaccacg gcgttccgca
     7501 agtcactttt gaagaagtat gtgaccatct tggccgaagt ggaaggggaa gatgaagtgc
     7561 gctacatcca cggcaactac ggccgcggca ctttcacttt ctactccggc cacgacccgg
     7621 aagactacca gcacttcatc aacgacccgc cgacggacct ctcattgcac aagaactccc
     7681 ccggctaccg gctgattttg aacaacatct tgttcccggc agcgcgcaaa aaagagcgca
     7741 aaacctaaaa aaccaagaga tcaacccctc ttacgttgta gcccccctct cttcagcttg
     7801 agtcccgcct cgggcgaatc gaaggccaga acggggaaat tatagaataa gaaaatgcgc
     7861 ttgtaggaac gaaccgctat aacccggcaa ctgacttaca atccctcgaa taagctcgct
     7921 aacttcgctc ccagcgcgct cgctagcttg gtcaaggttt ccagagaggc cgaactctgc
     7981 gctcgctcga tctgggaaat caaactgacc gatacgtcgg cccggttggc cacctgctgc
     8041 aaagtcaggt cctttttcaa gcgcagcgcc cggagccgtt cgccgatttt cagatttacg
     8101 cgctcggcca aatcggtcac cagcccgcgt tcgaccactg ctcgctccac caccggccaa
     8161 aggtcagctg gcttggacgg tttcttgagg tagtcaaaga ctcccaactt gaaactggcc
     8221 accgcctgct ccaccgtcgg ctggtcagtg agcataatga cgcatagatc agtatcgtgc
     8281 ttccggattt cccgcaacag gtcaagcccg cttttgtccg gcagcgtttg atcgatgcag
     8341 acgatgtgcg gattttcgct gtggattttg gcgagcgccg tggcggcctt gccggtcacc
     8401 tgagcctggt aacccttttg caccagggct tgtcctaatt ctgccgcctg cggctggtcg
     8461 gcagtcacca ccagcacctt cactttgcgt tggctggtca taaaacacct ctttttcttt
     8521 ctgcccgtag agtatacgtg aggtattcaa ctacgttgta aaaataggag ggagcttaaa
     8581 gggcagctga acttttttcc ggttcgacgg gtcctggcac cgagacccag acctgcccgt
     8641 tttcgatttt caccgggtag gttttgagct tggcccagga cgtagtaagg cactgaccgg
     8701 aggtaagatc atactgccac ccgtgccagg agcaggtaat ggtttggttg ggttgcacct
     8761 gcgcgaattc cagaggcgcc ttcatatggc ggcaaagtcc ttccacggca aaaagatctc
     8821 cgctgatttt gaaaagaaca ataaccgcca gacccacgcg cacccgtttc ccctcccctt
     8881 cggagatatc cgcaagtgcc gccacccgga cgtactggag atcgctcgtc atttcttgcg
     8941 agactaccgg cgcagctgat ttttgtcaag gtcggcccgt ctgttccgat ctccgtgatg
     9001 ggctcccttg taccatcgcc acagaatttc tatgtttaag aaatgctcca ggtcgccgct
     9061 gcggtcatcg tccaaaatgg aaaaattttg atcggccaac gcaaaaaaac cgaccgcttt
     9121 ccgctcaaat gggaatttcc cggcggcaaa gtacagtcta aggagtcacc ggaaaccggt
     9181 ctcctgcggg aactcagtga agaattaggg attgaagcgc agatcggcaa caaactaacc
     9241 gaagtgacct acgcctaccc gaactttggc ccggtgcgga ttcagttttt caccgtcaca
     9301 cggtaccatg ggaccctgga gaaccgggcg tacgaacaga tcgcctgggt caatcctgcc
     9361 caactgccgg actacgactt tcttgaggcc gaccgatcgt tgatccggga actggcacgc
     9421 agtacggtag ccgggggtag atagaaagag cacctgtgaa agcaatttcc acgaaaaaaa
     9481 agaacagatt ccgaaaaaac ttgcggcgct ggtttgtccg aaatagacgc gacctcccct
     9541 ggcgccgaac ccggaatcca taccgcattc tggtgtccga aatgatgctc cagcaaactc
     9601 aggtggaacg cgtcctgccg aagtaccggg agttcctcaa aaaatttccg acgctgactt
     9661 ctctggcaaa agcgccggtt gctgcagtga ttagatgctg gtgcggcttg gggtacaacc
     9721 agcgggcggt ctacctccac cgagccgctc gactgttaat cgagcgaaac ggcggaaaat
     9781 ttccccgcgt gcctgtcgaa ttacagaaat tccccggcgt tggtccctat accgccggtg
     9841 ccgttgctat ttttgcctgc aatcgagatg tcgtgatgct ggacaccaac atccggcggg
     9901 tgctgaccag agtgtttgcc ggtcagcgac ctgtatccga aaaaaaactc cataaactgg
     9961 ctttccggct cttgcccgct ggccggagca aaatctggca cagcgccctt atggatctgg
    10021 gtgctttact ctgcaccacc cgaaatccaa agtgccctct ctgttcggta caacgtgatt
    10081 gcctttggta tcgggaatct cagcgaaaac ctcggcccgc gagccgtccc gcacgacgaa
    10141 aacaaccgcc atttcgcaat tccacccgct tctggcgcgg acggattgtg gctgcactgc
    10201 gggaactttc caatggccga aagttttcgc tttttcagtt ggggaaacgg atcaagccga
    10261 atttcaaaac cgccgacctg ccctggctca aacgactcgc taccgcttta gtccgcgacg
    10321 ggctggcaac ccagagcggc cggcaaatct cgctcccgac gtaattgcag ccacgctctt
    10381 ttgagcgcgg tgaaagttga ggcattggga ataataatcg cacagcagat gaagcagatt
    10441 gagcagatct tctctgtcgg atatgcttca tccgctccct ctgctgcgaa tgccttcttt
    10501 ctaaaaccgc actaccaacc ctgctcgatt tccggatagc aaccctacct gtactttcct
    10561 ttcccctttt tcccggctga ccacctgccc cacccacgta gccatcgccg cggcgaagaa
    10621 tacgtccgcc gggtagtgca caaaatcatt caagcgtgcc agagccaccg taccggccaa
    10681 tgtgaacgcg ggaatcgtga cgtatggctt ccggtacgtc ctacttatag ccgaagccag
    10741 ggcaaacgcc gttgtggtat ggccggaagg aaaggaaagt ccggtttcag gtgaatgcca
    10801 ggtcgggccg tcaaagtgcc cggggttctg gagcgcgccc ggacgccggc gattcaatcc
    10861 gtgcttgagg acctgcgtga ttccctgcgc tatggccacc gctttgaagc tgttccacgc
    10921 cgcgcgccgt gaggttggat gccggaaaac gatgccgccg acgtaaagcc cgcccattgc
    10981 cagactggcg ccctcgcggc tgcccaaagg cgttacccag cgcgccagat catcggagag
    11041 gtttgagcgg cgccgggaaa tttccgcttc aacgtcgtga tcggttgcca gaaggccgac
    11101 ggtaactgcg ccggcgctgg cgcaaaccac ccaatccttc ttctgccagt gggtcggagc
    11161 gaaaacgacc gatttcgtgt cggtccagaa ttctgataca agatggtcga gggctttcag
    11221 cgtggagttc tgtgcggcac ccgccgtctg attcgcgtgg acggaatccg ccggctcagc
    11281 ctgagccgaa gtaaagcaac ccgcgctggc cgggaggaaa caaaccgaaa gcccgaaaat
    11341 ctgtgagatt ttcaaagcgc ttctgcgggg ctgaccggtt cagtcttcac aattccaggc
    11401 cgagttccgt caaaagctgc aggaaaattt tttcgccccg gccggagaac cggaaaccga
    11461 cgccgacttt ggccgtcact tcagccggca aaacgaggtg gacctgcggc acaagatagt
    11521 ggggagcagc gtgcccgaac actccctggg cttcggcgcc aatgcgcaaa agatgtttgg
    11581 tatcgtatcg cgtcgcgatg gcatagccaa actcggtttc gtgcccggcg tttaactgct
    11641 gctccagcga aaagtcgagg gtgaaattca accgctggag atccttagaa aggactatag
    11701 ttggttccag actgtggata gaaacccctt ctgcattgga gtggctatac tccaatgaaa
    11761 aggccggatc gagaaacttc tttccttcat caaaaagtcg aacccgtgtc tcaacatgcg
    11821 tggctacgta gcccagattc ctgccgggaa ggtgttgaaa agtggaaaaa gcttcgacca
    11881 tccagtgctc gagaatgccg tactcgaaag atgcggtgtg ctgccaggta ttcagaactg
    11941 aacgtgagac gctggttgaa ccgtagtgcg cccagtactc aagttccagt tcccggtggt
    12001 ctaaggtctg gtagacgaag gtcttgttga ggtaatcttc gcggtgcgcg aatgcaaccg
    12061 tcggagtcaa caataagaag aacagaacga ctcctgaata cgtgcggact gaccgtctgg
    12121 ggaaaagggg catctctatt tcctacagca tcattggagg aaaagtttgt gtgaactctt
    12181 cacttcgccg gtttccagag ttggtactga tttccctcgg gatcggcgaa ccagacgtac
    12241 ttgccccagc tttcctctcc cacgggtgtt accagccgca cccctttgct tttcagccgg
    12301 gccacttcgg cttcgatatc gtccactttg aacgaaagcc gcagtggttg ctgctctctg
    12361 tctttgatac ccgacctacc cggtacgccc acatccaagc cgctcttttg cggcccgaac
    12421 agctcaatca gaacctcacc gagtttgaag ccggcccagt cgcccttgct gtcgaagaga
    12481 tgaaggccca gcgagtcgcg caggaatgcc ttcactctct cgatttcctt gacataaatc
    12541 aaaacggctc cgacactttt cattgcacct ccctcgccgg gggaatcaac aacaatactc
    12601 tcaggtgaga tactcccgaa tgcccgcttc gagaggtaat aaggcaatcc cgaacgtccc
    12661 gatcagcttg cggttgtcgc acacgttgtc ctcctggagc attatcaact gctcgctggt
    12721 aacaggagga ttggtgagca tgatttccaa gagccgcacc accggacgca tcagtgccag
    12781 cgggagatga attttcattc ttttcttcag catgactttt gcaacgacgt cgaggagctc
    12841 attgtaggta taggcttggg gcccaccgat ctcaaaaatc tggttttgcg cccgcggatc
    12901 ggctagcgac ttaacagcgc aggcagcgac atctttcacc gacacgggct gcaacttcga
    12961 taaacccgaa ccgatcaccg gcaccaccgg gttgtaccgg atcagttttg ccaggagatt
    13021 gatgaacgca tctctcgggc cgaagatcac cgatggtcgg aaaaccgtgt acggtattcc
    13081 gctttgaatt attatctgtt ctgcttgcca cttggttcgg tggtaggtgg aacgggcatt
    13141 ttcccgcgta cccgcagcgc tgatgtaaat aatctgcctg acacccgctc gtttagcctc
    13201 agccaccaga ttccgagtgc cggtgacaac taatttttca aaggtttgtt ctttcctttc
    13261 ccgaatgatg ccaattgcat gcaccaggac attaacaccg gcaaaggcag aagttaggga
    13321 cgccggctcg gcaaggttcc cgtggcagat ttcagcgccc agccgcttca gttcccgaac
    13381 cgttccggag acgtcgcgcg taccgcgcgc caggcagcgc atcgtgtgcc cccgggcgag
    13441 cgcttgccgc gcgatttccg atcccacgaa gccggtgccc ccggcgacaa gtatattcac
    13501 agagatcctc ggactctcct gccaagatag acgttagaaa aaccgcgtca atcccgcgcc
    13561 atgtacccaa aaatctcttg cgtaccgcag gctccatttc gtattttata attgcgaatg
    13621 cggcaggcat ccttccacta cgacgccagg gagcatgccc tcaggaacct aaagaaggcc
    13681 tgcctgaact aaacgagatg gaacgaacca ccaccatcag cggaaccggc cacgccgtgc
    13741 cggaaaacgt gttgaccaat caggatttgg aaaagatggt cgacacctcc gacgagtgga
    13801 taattgagcg cacgggcatc cgtaagaggc gtatcggcaa cgccactacc actgcct
//
LOCUS       DBNGZD010000148         5038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_581348, whole genome shotgun sequence.
ACCESSION   DBNGZD010000148 DBNGZD010000000
VERSION     DBNGZD010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_581348"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="AC16GM_04090"
     CDS             complement(<1..2026)
                     /locus_tag="AC16GM_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2922890.1"
                     /translation="MTHQMKAVIMAGGFGTRLRPLTATLPKPMVPVGGVPLMEHVVRL
                     LARHGFSEVIVLLYFQSQAIRDYFGDGKEFGVRLEYIQPTEDFGTAGSVKLAAERLPE
                     EFLVISGDVCTDIDLAQAAAFHRTKQAQATMILTRRENPLPYGVVVTEPDGRITQFLE
                     KPTWGQVFSDTVNTGIYILQHDVLSAIPAQLRFDFSQNLFPLLLERKSALFGYVALGY
                     WRDIGNLEEYFQIHRDLLAEKVQLEIPLERKRTPSATLWVGSNVQIDPPAQFSGRVII
                     HRGARIDAQAAVANTVIGAGTVVGPEAAVKDSILWERVTVGERASLTGCVVASDAVIG
                     PEARVGEQVIVSEGTTIGRRAKILPGVKIWPGKKVEDEAVLGTSLIWGESWARELFSG
                     AKVAGLANRELTPEFVVKLGAAYGAFLGEGATVVTSRDASKVSRMLNRAVICGLVSAG
                     VHAQDLRTLPIPVVRYELKNGSQKGGLHVRHSPRDPATVEIVFFDAGGQDLPPGKTKA
                     MERLFFREDFRRAEPASVGQLDFPVRVIEHYREGFLQNIPAETIRQARFKVVVDYSWG
                     GAAEVLPGILGHLGLDTVSLDAYVDPQKLFQAEVVEKTGWTQLATIVRSVGADLGLWL
                     DPGGERLALVDEAGAVISGQRLLALFTFLVLQGLPVKELAVPVMATMAVEK"
     gene            complement(2023..2754)
                     /locus_tag="AC16GM_04095"
     CDS             complement(2023..2754)
                     /locus_tag="AC16GM_04095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HIR2922891.1"
                     /translation="MNVEQKSPLPLARSSPWIVCVDFDGTVTEGDVGNRFSVLFSGGR
                     TRPLVERWLAGSITARECLEEECKLLNFDAAQFERFLAEQKITPGLHEFVRFLEEQGI
                     PLYILSDGLDLYISRLLEREGLGRIPVFVNRARIEGGRVIPEFPYLDQGCGRCGNCKK
                     YHIQRLRQPGQKVLFVGDGYSDRCAAEAADLIFARGDFLQYCRERNIAAIPFASFFEI
                     AANVKTILGGETPVAPLRAKKAVVL"
     gene            complement(2751..3053)
                     /locus_tag="AC16GM_04100"
     CDS             complement(2751..3053)
                     /locus_tag="AC16GM_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019459.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HIR2922892.1"
                     /translation="MNTQSLDRTVGKLLLVGSWLAVICLGLGLVILLVGGTLAQGNSG
                     KVLLTAGLVTMLATPVGRVLAALFAFLRQRDWPYATVAGGVLGTLILGFLIGLVTR"
     gene            complement(3055..3870)
                     /locus_tag="AC16GM_04105"
     CDS             complement(3055..3870)
                     /locus_tag="AC16GM_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR2922893.1"
                     /translation="MSGVEPLALVLGGAAAGLFGSLLGLGGGLLIIPYLTLVFNVPMH
                     TAIATSLICVIATSSAAAAVYVQRHLCDIRLGMVLELATTTGAVAGGLFANILNPNVL
                     ATLFAGLLFFTAWTMWRRREPLPEAAIAPNADPTIPPVRPTYRHLPGGLGISFLAGNL
                     SGLLGVGGGILKVPAMHLVMGVPLKIATATSNLMIGVTAAASGFIFFLRGRVDLPLAA
                     WIVFGVFWGATLGARWAAKVKTKTLRLVFVLVLVVVGIEMASKGLSVPLPWQR"
     gene            complement(3873..>5038)
                     /locus_tag="AC16GM_04110"
     CDS             complement(3873..>5038)
                     /locus_tag="AC16GM_04110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR2922894.1"
                     /translation="KRDEIEVKAVPYAGLTEEGIGYVRLTRFSESAPKELAEALTFLQ
                     TQNPKGIILDLRSNPGGLLGAAVGVAGLFLGGEKLVVETRGRRDEQNKKYTAGATALC
                     PDLPLVVAVDEYTASAAEIVAGAIQDWDRGLVIGRPTFGKGLVQTPIDLPNGAALKLT
                     TARYFIPSGRTIQRPDRARALSLVEADSAADSSAHQPFHTKGGRTVYGMGGVVPDLAV
                     ERTPRTPVEVALIPYFFDFAVYYLAGHPEVPADFTPDAAVRAQFKKFLAEKKFSYRSQ
                     TEDDLALLEGKAAQEGYSLSLRQAIRALRGRINKEKEQALAGESEFISWQIKEQVLGA
                     RYGERAIYPAVWLHRQPELLKAREILTRSGEYRKLLALDSGQSGAIAPPRVAR"
BASE COUNT         1069 a         1609 c         1436 g          924 t
ORIGIN      
        1 atttctccac cgccatcgtc gccatcaccg gtaccgccag ttctttgacc ggcaaaccct
       61 gcaaaacgag aaaagtgaaa agtgccagca acctttggcc ggaaatgacc gcaccggctt
      121 catcgaccag cgccagccgt tccccaccgg gatcaagcca gagacccaag tcggctccta
      181 cagagcggac gatggtagcg agctgggtcc agccggtttt ttccacgacc tccgcctgga
      241 agagcttctg cggatcgacg tacgcatcca aagagacggt atccagcccg aggtggccga
      301 ggatgccggg gagcacttcg gccgccccgc cccaggagta atccaccacc actttgaacc
      361 gtgcctgccg gatggtttcg gcaggaatgt tttgcaggaa accctcccgg tagtgttcaa
      421 tcacccgcac cggaaagtcg agctgtccca ccgacgcggg ttcggcgcgg cgaaaatctt
      481 cccggaagaa gagccgttcc atcgccttgg ttttcccggg cggcaggtcc tgtccgccgg
      541 cgtcgaagaa gacgatttcc accgtggcgg gatcgcgcgg ggaatgccgc acgtggaggc
      601 cgcctttttg agatccgttc tttagttcat agcgcaccac cgggatcggc aaggtgcgta
      661 aatcctgggc gtgcaccccg gcggagacta acccacagat gacggcccgg ttgagcatcc
      721 gcgacacctt actggcatcc cggctggtga cgacggtggc accttctccc aaaaatgcgc
      781 cgtaggcggc acccagcttg accacaaatt ccggcgtcaa ctctcgatta gccagccccg
      841 ccactttggc accgctgaaa agctcccgcg cccagctttc accccagatt aaactggtgc
      901 cgagtacggc ttcatcttcc acttttttcc cgggccagat tttgacgccc ggaagaattt
      961 tagcccgccg gccaatggtg gtgccctcac tcacgatgac ctgctcgccg acccgcgcct
     1021 ccggcccgat cacggcgtcg cttgccacta cgcagccggt gagggaagcc cgctccccca
     1081 ccgtgacccg ctcccagagg atgctgtctt tgactgccgc ctccggcccc acgaccgtcc
     1141 ccgccccgat aacggtattg gccacggcgg cttgagcgtc gatccgcgca ccccgatgaa
     1201 taatgacccg gccggagaat tgagccggtg ggtcgatctg gacgttgcta ccgacccaga
     1261 gtgtcgcgga tggtgtcctc tttcgctcca gggggatttc cagttggact ttttcagcca
     1321 atagatcgcg gtggatttgg aaatattcct ctaaattacc gatgtcgcgc cagtacccca
     1381 gagcgacgta accgaaaagt gctgattttc tctccagcaa gagcgggaac aggttctgac
     1441 tgaaatcaaa gcgcagctgg gcgggaattg cggacaaaac gtcgtgctgg aggatataga
     1501 tgccggtgtt gaccgtgtcg ctgaagacct gtccccaggt cggcttttct aaaaactgcg
     1561 tgatccgacc gtccggttcg gtcaccacga cgccgtacgg caatggattt tcccgccggg
     1621 ttaaaatcat ggtcgcctgg gcctgcttcg tccggtgaaa agcggccgcc tgggcgagat
     1681 cgatgtcggt acagacgtcg ccggagatga ccagaaactc ttcggggagg cgttcggcgg
     1741 ccaatttgac gctccccgcc gtgccgaagt cctcggtcgg ctggatatac tccagccgga
     1801 cgccgaactc cttcccgtcg ccgaagtagt cccggatcgc ctgcgactga aaatagagga
     1861 gcacgatcac ctcggaaaaa ccgtggcggg ccaaaaggcg caccacgtgc tccatcaaag
     1921 ggacgccgcc caccggcacc atcggtttgg gaagggtggc ggtcaaaggc cggagccgcg
     1981 tgccaaagcc gccggccata attaccgcct tcatttggtg tgtcatagca ccactgcttt
     2041 ctttgcgcgg agcggtgcga ccggcgtttc acccccgaga atggttttga cgttggccgc
     2101 gatctcaaaa aatgatgcga aaggaattgc agcgatattg cgctcccgac agtattgcaa
     2161 aaagtccccg cgagcgaaaa tgagatccgc cgcctccgcc gcacaccggt cggagtagcc
     2221 gtcgccgacg aagagcacct tctgtccggg ctggcgcagc cgctggatgt gatatttttt
     2281 gcaattcccg caccggccgc agccctgatc aaggtaggga aattcgggaa tgacgcggcc
     2341 accctcgatg cgcgcgcgat tgacgaaaac cggaattcgg cccaagccct cccgctccaa
     2401 gagccgcgaa atgtagaggt ccaatccatc gctcaagatg taaagcggaa tcccctgctc
     2461 ttccaggaac cggacgaatt cgtgcaagcc cggcgttatt ttctgctccg ctaagaatcg
     2521 ttcaaactga gccgcatcga agttgaggag tttacattcc tcctccagac actcccgggc
     2581 ggtgatactc cccgccagcc agcgctccac gagaggccgg gtgcggccgc cggaaaataa
     2641 gactgagaaa cggttgccca catcaccttc ggtgaccgtg ccgtcaaaat cgacgcagac
     2701 gatccagggt gatgatcgcg ctaagggcag aggtgatttt tgctccacat tcatcgggtc
     2761 accaagccga taaggaaacc gaggatgagt gtccccagga cccctcccgc taccgtcgcg
     2821 taaggccagt cgcgttgccg caagaaagcg aaaagcgcgg ccagcacccg tcccaccggc
     2881 gtggccagca tcgttaccag accggcggtc aaaagcactt tccctgaatt cccttgagca
     2941 agtgtgcctc cgacgagcaa aatgacgagc cccaatccga gacagattac cgccagccag
     3001 gatcccacca atagcaattt gcccactgtt cgatccagcg attgagtatt cacgttacct
     3061 ctgccagggc aaaggcaccg aaagtccctt gctcgccatt tcgatgccga ccacgaccaa
     3121 aaccagtacg aaaaccagac gcaaagtctt cgtcttgacc ttggccgccc agcgcgcgcc
     3181 caaagttgct ccccagaaaa cgccaaaaac gatccaggcc gccagcggca gatcgacccg
     3241 gccccgcaag aagaaaatga atccagaagc ggctgccgtg accccaatca tcagattgct
     3301 ggtggccgtg gcaattttca gcggcacacc catcaccagg tgcatcgccg gcactttcaa
     3361 tattcctccg cccactccca gaagtccgga aagatttccg gccaaaaatg aaattcccag
     3421 cccgccgggc aggtgccggt aggtcggccg tacgggaggg atggtcggat cggcattggg
     3481 cgcaattgcg gcttcgggca aaggttcccg acgccgccac atcgtccaag cggtgaagaa
     3541 cagcaggccc gcgaatagtg tcgccagcac attcggattg agtatgttgg cgaaaagtcc
     3601 gccggcaaca gcgccggtgg tagtagccag ttccaagacc attcccagcc ggatgtcgca
     3661 gaggtgcctt tggacgtaga ctgccgccgc cgccgaagag gtggcgatga cgcagatcaa
     3721 actggtggcg atggcggtgt gcatcggcac gttgaataca agtgtcaggt agggaatgat
     3781 caaaaggccg ccgcccaaac ccaaaagcga accgaagagc cccgccgccg ctccgcccaa
     3841 gaccagtgcc agaggctcta cgccggacat gttcagcgag cgaccctggg cggggcgatc
     3901 gccccacttt ggccggaatc gagtgccaaa agcttccggt attctccgga gcgggtgaga
     3961 atttcccggg ctttcaaaag ttccggctgc cggtgcagcc agaccgccgg gtagattgcc
     4021 ctttccccgt aacgcgcgcc gagcacctgt tctttgatct gccaggaaat gaattccgac
     4081 tcaccggcca gcgcctgttc tttctccttg ttaattctgc cgcgtaaggc gcggatggcc
     4141 tgccggagag agagggaata tccttcttga gcggcttttc cctccaggag agctaaatca
     4201 tcttcggtct ggctccggta ggaaaacttt ttctcagcca ggaacttttt gaactgcgct
     4261 cgcaccgccg catcaggggt aaaatcggcc gggacctcgg ggtgacccgc caggtagtaa
     4321 acggcaaaat cgaagaagta cggtatcaga gccacttcca ccggcgtccg gggtgtacgc
     4381 tcgaccgcca gatcgggcac cacgccgccc atgccgtaga ccgtgcgtcc ccctttggtg
     4441 tgaaatggtt gatgcgccga ggaatcggca gcggagtcgg cttccacaag agagagcgcc
     4501 cgcgcccggt ccggtcgctg aatcgtccgc ccggaaggga tgaagtagcg ggcagtggtc
     4561 agcttcaagg ccgccccgtt cggcagatca atcggcgtct gcaccagtcc tttgccgaac
     4621 gtggggcggc cgatcaccag accgcgatcc cagtcctgaa tggcgccggc cacgatttcc
     4681 gccgccgagg cggtgtactc atccaccgcc acgaccaaag gcaggtccgg acagagggcg
     4741 gtggccccgg cagtgtactt cttgttctgt tcatcccgcc gcccccgggt ttcaacaacc
     4801 agtttttcgc cacccaaaaa caagccggcc acccccaccg ccgcgcccag taaccccccg
     4861 gggttgctcc gcaggtcgag gatgataccc ttgggatttt gcgtttggag aaaggtgagc
     4921 gcctcggcta actctttggg cgccgattcg gaaaagcggg tcaaacgcac gtagccgatc
     4981 ccctcctccg tgagaccggc gtacggcacg gccttgactt cgatctcgtc ccgcttca
//
LOCUS       DBNGZD010000149         4080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_442055, whole genome shotgun sequence.
ACCESSION   DBNGZD010000149 DBNGZD010000000
VERSION     DBNGZD010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_442055"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="AC16GM_04115"
     CDS             complement(<1..1773)
                     /locus_tag="AC16GM_04115"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR2922895.1"
                     /translation="MTARRLFLTVGLGVAAGFLLIASDRPAGPMLVRAYFPDRQTFQK
                     IQALDLDIAEVNAQGLFCDIVASSSDLARLQGAGIRTEVTIRDLTLYYTNRTLATMGG
                     FHTFSEVVAALDSIHNEHPTITTAKDSIGTTIEGRTIWAMKISDNPGVDENEPEVFFN
                     GLTHAREPIGMEICLNTMRYLTNNYGSDPQVTSLVNDRELWFVPVVNPDGYVRNQTTN
                     PSGGGMWRKNRRNNGDGTFGVDLNRNYGYQWGYNNVGSSPNTSSDVYRGTAPFSEPET
                     QVIRNFCNSHNFTICLNYHSYGNLFLAPWGYTTEFPEDYDLLRAIGDSAVQSNGYISQ
                     PGWGLYFTNGDADDWQYGERSTKAKTISYTPEVGRNSDGFWPTLARIPVLISENLAPN
                     LFVARIADNPWRLLPPLPPTLTLGGTEVDTAGYVIHWSHSDPNNPAALFEVHELQSPY
                     LATDGAELGLARWRADGFTMTNARSFSGVASFYGGNANATVTTLTAKEGYLVSSVADT
                     LRFHTWYDTEVDYDYAYVEVSTDQGRTFTPIPGNITTDSNPNYLNRGNGINGSSGGLW
                     GEAKFPLGSYAGQRILIRFTYITDP"
     gene            complement(1947..>4080)
                     /locus_tag="AC16GM_04120"
     CDS             complement(1947..>4080)
                     /locus_tag="AC16GM_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2922896.1"
                     /translation="FREKRNTKDVMSVKILGGSVPSESVSYNWWISEGTNPVDQDWGP
                     QRLPGRRNYSGGYGTPEGDVGKYRYMSEGEKDYDQLTLELPHPGWIPPPKNADSLVDG
                     FDTRFLLSVGPFDIAPGESVQVGFAVVAGKDFHVGANDFRNTFPPGNFSGFKDSATVA
                     AFYSLLNFDSLVATVQLAQATYASNYRLPPPGPPEGLRVDTWTDSTVTLGWNPSGIVD
                     VFGYNVYRSTDSTFAGPPLNATLLPTNGFVDTTVTRGVLYLYAVSAVDSGLAEGKKSL
                     PVSVLAGQPEIPQGLSAVGGRGEVTLFWAPNPEPDIDHYNIYRSTAPNLPFGNIGSTT
                     GAETTFVDQTVQNGVVYRYQISAVRVDGLASFLSPIVFGVPMAFDQGLGLVDLTFNGF
                     ANLVNHDSVIAFYNRALRGYQFSLLNFSASIDATPSLANLSPYPVLLLASEDLRYPLP
                     DSLFRVLSFYRIAGGKVLISGQANFSFYSYTDPNRYNDIFNLSGLPPQMLFAVDSAYA
                     PRPAIYLAFTDSLKVQREFIGAQPKVQGYPLLEVDTARLNRYLCVPFVPPRCLAHNGT
                     LPAVEYFFSADTSEDIYTFVSRYPDTSHAHGKAVAKRKITPGYSYVFFDLPLFFMKEA
                     EAKAALKAGLADLGLTPTPYTVGDVNNNGAIDIADIAQLVQFVVFATNPPQPFEFVGD
                     TNCDGLIDIVDIVLLLQYVLYGQPPLCL"
BASE COUNT          914 a         1116 c         1194 g          856 t
ORIGIN      
        1 gggatcggtg atgtaggtga agcggatgag aattctctga ccggcgtaac tccccagagg
       61 gaatttggcc tccccccaca ggccgcccga ggaaccgttg atgccgttgc cccggttcaa
      121 ataattcgga tttgaatccg tcgtgatgtt gcccggaatg ggggtgaagg tccgtccctg
      181 atcggtggag acctcgacgt aggcgtagtc gtagtcgacc tcagtgtcgt accaggtgtg
      241 gaagcgcagc gtatcggcca cgctggacac aaggtaccct tccttcgccg tcaaagtggt
      301 gacggtggcg ttcgcattcc cgccgtagaa gctggcgacc ccggaaaaac tgcgggcgtt
      361 ggtcatcgta aagccgtcag cccgccagcg ggccagcccc aattccgcgc catcggtcgc
      421 cagataggga ctttgcagtt catggacttc aaacagtgcc gctgggttat tgggatcact
      481 gtggctccag tgaatgacat agcccgcagt gtcaacctcc gtacccccga gtgttagagt
      541 cggcggcaag gggggcaaga gccgccaggg gttatccgca atgcgggcca cgaagagatt
      601 cggggcgaga ttctcactga ttaaaaccgg aatgcgggcc agagtcggcc aaaaaccgtc
      661 gctattccgc cctacttccg gggtataact aatggtcttg gctttcgtgc ttctttcccc
      721 atactgccag tcgtcagcgt cgccgttggt gaagtacagc ccccagcccg gctggctgat
      781 gtacccgttg gactgcacgg cgctgtcccc gattgccctc aggagatcat agtcttctgg
      841 aaactcggtg gtgtaacccc agggggccag gaagagattc ccgtaggaat ggtagttgag
      901 gcagatggtg aaattgtggc tgttgcagaa gttgcggatc acctgagtct ccggctcgga
      961 aaaaggggcc gtaccgcgat agacgtcgct ggaagtgtta ggactggagc cgacgttgtt
     1021 gtagccccac tggtagccgt agttccggtt caaatcgacg ccgaacgtgc cgtccccgtt
     1081 gttgcgcctg ttcttgcgcc acatcccgcc gcctgagggg ttagtcgtct ggttgcgcac
     1141 gtagccgtcg gggttgacca ccggcacgaa ccagagctcc cggtcgttca cgagtgacgt
     1201 cacctgtggg tcactgccgt agttgttggt caggtaacgc atggtattca gacagatttc
     1261 catcccgatc ggttcgcgag cgtgggtgag cccgttgaaa aagacttccg gttcattttc
     1321 gtcaacgccg gggttatcgg aaattttcat cgcccagatg gtgcgtcctt ctatggtcgt
     1381 cccgatgctg tccttggcgg tggtgatggt ggggtgttcg ttgtggattg aatccagcgc
     1441 ggccaccact tcactgaacg tatggaaccc acccatggtg gcaagcgttc ggttcgtgta
     1501 atagagggtg agatcgcgga tggtcacttc cgtacgaatg ccggctccct ggaggcgagc
     1561 caggtcggat gacgaagcaa caatgtcgca gaacaaaccc tgggcattga cctcggctat
     1621 gtctaaatca agcgcctgga ttttctggaa agtttgccgg tcgggaaagt aggcccgcac
     1681 cagcatcggg cccgcgggcc ggtcactggc aataaggaga aaaccagcgg ccacacccag
     1741 cccgacggtc aggaaaagtc ttcgcgctgt catcacattt cgctccgcat caggtcagcc
     1801 agtacgtgaa agcgcgacct ctttctggct tcgccgcgcg gggagaatat aacagttttt
     1861 agaccccatg caagaaaaat tttcgctggt ctggcccccc ctgcagcggg cggcaggaag
     1921 agttgcgggc aaccctcggt gggaggttac agacacaaag gcggctggcc gtacaagacg
     1981 tactgcagca gaagaacgat atcgacaatg tcgattaatc cgtcgcagtt cgtatcgccg
     2041 acgaactcga acggttgggg cgggttggtg gcaaagacga caaactgcac caactgcgct
     2101 atgtcggcaa tgtcgattgc cccgttgttg ttcacgtcac cgacggtgta gggagtaggc
     2161 gtcaaaccca gatctgccag cccggccttt agggcggctt tcgcctctgc ttctttcatg
     2221 aagaaaagcg ggaggtcgaa gaagacgtag gaataccccg gtgtaatttt gcgcttggct
     2281 accgctttgc cgtgggcgtg cgaagtgtcg gggtagcgcg agacaaaagt gtaaatgtcc
     2341 tctgaggtat cagcgctgaa gaaatactcc acggccggca aggtgccgtt gtgggcaagg
     2401 caccggggcg gaacaaacgg tacacacagg tagcggttga gcctggccgt atccacttcc
     2461 agtagcggat agccctgcac tttgggctgc gccccgatga attctcgctg aacttttaat
     2521 gaatcggtga aggcaaggta gatggcaggg cgtggagcgt aggcactatc tacagcaaaa
     2581 agcatctgtg gcggcagacc cgagagattg aaaatatcgt tgtagcggtt gggatcagtg
     2641 taactgtaga atgagaagtt cgcctgcccg ctgatcagaa cttttccacc agcaatacga
     2701 tagaacgaaa gaacacggaa gagcgagtcg ggtagggggt agcgcaaatc ttcagacgca
     2761 aggaggagga cggggtacgg cgagaggttc gccagagatg gcgtcgcgtc gatggaagca
     2821 gagaaattca ggagtgaaaa ttgatacccc cgcagcgcgc ggttatagaa ggcaataacg
     2881 ctgtcatgat ttaccagatt cgcgaagcca ttaaaagtca aatcgaccaa ccccaaaccc
     2941 tggtcaaaag ccatcggcac gccgaacaca atcggggaca agaaacttgc cagtccatcg
     3001 acccgcacgg cactaatctg atagcggtag acgaccccat tctgcaccgt ttgatcaacg
     3061 aaagtggttt cggctcccgt agtggaacca atgtttccaa acggaagatt tggagccgtg
     3121 gatcggtaga tattgtagtg gtcaatatcc ggctctggat tgggtgccca gaaaagtgtc
     3181 acctctcccc tgccgccgac ggcagacaaa ccttgcggga tttcgggctg tcccgccagc
     3241 accgataccg gaagtgactt tttgccttca gccaatccac tgtcaacggc tgaaacggcg
     3301 taaagataga gcacccctcg cgtcaccgtc gtatcaacga aaccattcgt cgggagtaat
     3361 gtcgcattga gcggcggacc cgcgaatgtc gaatctgtgc tccggtaaac gttgtagccg
     3421 aaaacgtcca ctatgcccga cggattccag cccagcgtga ccgtgctgtc cgtccaggta
     3481 tcgacccgca atccttccgg cggcccgggc ggcggcagcc ggtaattgga agcatacgtg
     3541 gcctgcgcca gttgcactgt tgcgacaaga gaatcgaaat tcagcaggct gtagaacgcc
     3601 gccaccgtcg ccgaatcttt gaagccactg aaatttccgg gaggaaacgt attccgaaag
     3661 tcgttggcgc ccacgtgaaa atcctttccc gccacaaccg caaaccccac ctgaaccgac
     3721 tcgcccggag caatgtcaaa cggcccaacg ctgagaagga aacgggtatc aaaaccatca
     3781 acgagactgt ccgcattttt gggcggagga atccagccgg ggtggggtaa ttccaatgtt
     3841 aactggtcgt aatctttctc gccttcactc atataccggt attttcccac gtctccttcg
     3901 ggtgtcccat atccaccgct gtaattacgc ctgccgggta accgctgtgg cccccaatcc
     3961 tgatccacgg ggtttgtccc ctccgaaatc caccagttgt acgagacact ctccgacgga
     4021 acactgccgc ccagaatttt tacgctcatg acatcttttg tattccgttt ctctctgaag
//
LOCUS       DBNGZD010000150         6593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_95244, whole genome shotgun sequence.
ACCESSION   DBNGZD010000150 DBNGZD010000000
VERSION     DBNGZD010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_95244"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2194
                     /locus_tag="AC16GM_04125"
     CDS             <1..2194
                     /locus_tag="AC16GM_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2922897.1"
                     /translation="KIVPFYDRSDLIHHAVSSLEHTLVEEAIIVSLVILIFLLHPRSA
                     FVVIISIPIAVLVAFISMYYLRITSNIMSLGGIAVAIGVLIDAAIIMVENAYRHLADL
                     PSGERREITPVVVRAAQQVGRPIFFSLVIIILSFLPVFLLEGQEGRLFRPLAFTKTTA
                     MAGAAILSITLVPVMMTFFLRGKLKPESANPVSRFFIKIYEPVLRLTLKHKWKTLLIN
                     ALVLVFTVFLTTRIGREFMPPLDEGSLLFMPITLPSVSITEAKRIVQIQDKIIKEFPE
                     VEAVLGKIGRAETATDPAPVSMIETIVLLKPKDQWRKGMNRVEIINELDRKLQIPGVS
                     NAWTMPIINRINMLSTGVRTDLGVKVFGSNLNIIEALGVEVEAALRKIPGAVDIVAER
                     VTGGKYLDIEIDRTRAARYGLNVGDIEEVIETAIGGERLTTTVEGRARFPVRVRYLRE
                     YRDDPEKLKRVFVPTPLGQQIPLEQLANIKVTVGPPEINSENSLLRGIVFLNVRGRDM
                     GGFVDEAKRVLSASIKLPPGYFLSWSGQWENQVRAKKRLEILMPAVIFIIFILLYFTF
                     HSVLEAFLVMLSVPFSLTGGVLLLWLLGYNFSVAVWVGFIALYGVAVETGVVMVVYLH
                     EALDRRLQRGTVDEAGLQEAIMEGAVLRLRPKLMTVFAALAGLLPIMWSSRTGSDVMR
                     PIATPMIGGMITSAIHVLIVTPVIFGIMKRHELRKGRLKPSGITLHHD"
     gene            2277..2747
                     /locus_tag="AC16GM_04130"
     CDS             2277..2747
                     /locus_tag="AC16GM_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922898.1"
                     /translation="MPKPLTPVPAPRRIPTAADSVVKIGSLRLSFSLYLWQDFMPIVP
                     PGGPPFHLSLEVTVKNQGTKPVTDFRLEKLSLYYDKSRRLFWTFRLASADSASGTLSI
                     APGAKRTFAYGPKSPGPRNIKLKEGTVLYGRVLGRVKGEKSIITLPPAGVNLTY"
     gene            complement(2797..4686)
                     /locus_tag="AC16GM_04135"
     CDS             complement(2797..4686)
                     /locus_tag="AC16GM_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025034.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HIR2922899.1"
                     /translation="MGRKNYSLQLPVRATHRLILWAVWFFSIWFISPGTGNANEIESI
                     LAKHAQALGGLEKVRALTTSHAQATVTAGGFTGKSEAYEAPPGRFRQDIDLGIFNLSV
                     GCDGTNWWIKDQNGIVRDLGEKEKEEFVTESFFSAYKYLTEDTATFTLRFLPEKSDSQ
                     FYAIAVKPQGGLGRTLFINRRTYLVDKSQQIQDGALITTTYADFRSVDGLLQPFHLHQ
                     SSGQVIFDTDIRITSVETNVAIDPAVFARPGAAARRDFAFAPGKTSTTVPFELVNNHI
                     YFSATLQGSVTGSFLLDTGAGAVVLDSTFAAKSGLVGAGGLEAKGVSGSEKAGLVKIG
                     SLTVGDVTFDSLVAATLDLQPLTQFEGHGIDGILGYDILARVVVEVDYEKSQLTFSDP
                     DLFTYTGTGQVLPMTLDQRIPHVEATVEGQYRGWFNVDTGSRNTLDLFSPFVFEHTLA
                     DKYPKRVETRLGGIGDDLPSTTQMVRMKSFTVGSFEIPDLLVGLSTAQKGIFASEKFQ
                     GNIGSGLLKRFTVIFNYPKQQLILFPNRHFGERDEFDKSGLWLREVAGKFIVEEVVKG
                     TAGDKSGFKRGDAITEINGKPAREYSLNDLRALFRGPDGTKFSLKFQRTNKEKKKELK
                     LKTIL"
     gene            4837..5277
                     /locus_tag="AC16GM_04140"
     CDS             4837..5277
                     /locus_tag="AC16GM_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922900.1"
                     /translation="MKKGLVFAGLLVLAFVFLFSSAFAQMSQPKKEEPKKEAMKMEMG
                     PKEMTVTGEVVDLACYIKMGARGAGHKECATECANAGIPLGIVDAKGEIFLPASKEDK
                     KGANAELLPFVAEMVTVKGKLYHKGGQRLLAIESVEKAAAAPAK"
     gene            5593..5979
                     /locus_tag="AC16GM_04145"
     CDS             5593..5979
                     /locus_tag="AC16GM_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922901.1"
                     /translation="MKRILTPVLAFVAVCAFTVMVFAQTPAADSQKAAPATKAQTKAP
                     AMKATTKPAAAKSLTFSGRVVSVDTVAMTVVVKGKKAEETFNVEPGTKVKVAGKTAKL
                     ADLKKDAWVKVMYKMKDGRRTATAIM"
     gene            6185..>6593
                     /locus_tag="AC16GM_04150"
     CDS             6185..>6593
                     /locus_tag="AC16GM_04150"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR2922902.1"
                     /translation="MPTETALAEGAPAPQFTLSADTGQKISLADFAGKTNVVLYFYPK
                     DDTPGCTKEACSFRDLKKEFEKAGAAILGVSMDSVDSHKKFKAKFKLTFPLLADEDGK
                     VTRAYGVYKEKNMYGKKYWGIERTTFVINKQGKI"
BASE COUNT         1450 a         1833 c         1906 g         1404 t
ORIGIN      
        1 aaagatcgtg ccgttctacg accgctccga tttgatccac cacgccgtgt cttccctgga
       61 gcacaccctg gtggaagagg cgatcatcgt cagtctcgtg attttgatct tcctgctcca
      121 cccgcgctcg gcttttgtcg tcatcatttc gattccgatt gcggtcctcg tcgccttcat
      181 ttcgatgtac tacctgcgca tcacgtccaa catcatgtct ctgggcggta ttgccgtggc
      241 gatcggtgtt ctgatagacg ccgccattat catggtggag aatgcctatc gacatctggc
      301 cgatttgccg tccggagaac gtcgggagat cacgccggtg gtggtccggg ccgcccagca
      361 ggtgggccgg ccgatcttct tctcgttggt catcatcatc ctctcgtttt tgccggtctt
      421 ccttttggaa gggcaggagg gccggctctt ccgaccgctg gcattcacga aaacgaccgc
      481 catggccgga gcggcgattc tctccatcac cttagtgccg gtgatgatga ccttctttct
      541 ccggggcaaa ctgaagccgg agtcggccaa tccggtctcg cgctttttca tcaaaatcta
      601 cgagccggtg ctgcgcttga ccctcaagca caagtggaaa acgctcctca tcaacgcgct
      661 cgtcttggtt ttcacggtct ttttgaccac gcgcatcggc cgggagttca tgccgccttt
      721 ggacgaaggg tcgcttttgt tcatgccgat tacgttgccc agcgtctcaa tcaccgaggc
      781 caagcggatc gtccagatac aggacaaaat catcaaagaa tttccggaag tagaagcggt
      841 gctgggcaaa atcggccggg cggaaaccgc caccgatccg gcgccggtct ccatgatcga
      901 aacgattgtg ctcttgaagc cgaaagacca gtggcggaag ggaatgaacc gggtcgaaat
      961 tatcaatgaa ctcgaccgca agctgcaaat tcccggcgtt tccaatgcct ggacgatgcc
     1021 gatcatcaac cggatcaaca tgctctccac cggggtgcgg accgatctgg gcgtcaaggt
     1081 cttcgggtcg aatctgaata tcattgaagc cctgggagtg gaggtcgaag cggccctgcg
     1141 caaaattccc ggagcggtgg acatcgtggc cgagcgggtg accggcggga agtacttaga
     1201 tatcgagatc gaccgtaccc gagcggcgcg ctacggcctc aacgtcggcg acattgaaga
     1261 ggtgatcgaa acggccatcg gcggcgagcg gttgactacc acggtggaag gccgggcgcg
     1321 ctttccggtg cgagtgcgct acctgcggga gtaccgcgac gacccagaga aactgaagcg
     1381 cgtcttcgtg ccgacgcctc tgggtcagca gattcccctg gagcagttgg ccaatatcaa
     1441 ggtcaccgtc gggccgccgg agatcaattc cgaaaacagc ctcctgcgcg ggattgtctt
     1501 tttgaatgtc cggggccggg atatgggggg attcgtggac gaggccaagc gggtgctatc
     1561 ggcttcgatc aagctcccgc ccggctactt tctctcttgg agcggacagt gggaaaacca
     1621 ggtccgcgcc aaaaagcgcc ttgagatcct gatgccggcg gtgatcttca tcatcttcat
     1681 cctgctctac ttcactttcc actcggtcct cgaagcgttc ctggtaatgc tctcggtgcc
     1741 tttttccctc accggcggcg ttctgctcct ctggctctta gggtataatt tctcagtggc
     1801 ggtctgggtc ggcttcatag cgctctacgg tgtggcggtg gaaaccggcg tggtgatggt
     1861 ggtctacctc cacgaagcgc tggaccgtcg tctgcaacgg ggaaccgttg acgaagccgg
     1921 gctgcaggaa gcaatcatgg agggggcggt gctccgcctg cggcccaaac tcatgaccgt
     1981 ctttgccgct ttagccgggc ttttgccgat tatgtggagc agccgcaccg ggtccgacgt
     2041 catgcggccc atcgccaccc ccatgatcgg cgggatgatc acctccgcca tccacgtctt
     2101 gatcgtcacg ccggttattt tcggcattat gaagcgccac gaactgcgca agggccggct
     2161 gaaaccttca gggattacgc tgcaccatga ctaaaagacg ctgggaaata gtcctgctgt
     2221 taacactggt tttcttcacg accccgcttt tgggcaaaac gagaaaaggc gcatacatgc
     2281 ctaaaccgct cacccccgtg ccagcaccca ggcggattcc gaccgcggca gattcggttg
     2341 taaaaatcgg gagcttgcga ctgagttttt ccctctacct ctggcaggat ttcatgccca
     2401 tagtaccgcc gggcgggccg ccgtttcatc tctcactgga agtgacggtc aaaaatcagg
     2461 gcaccaagcc agttaccgac ttccgactgg aaaaactctc gctctattat gacaaaagta
     2521 gaaggctctt ctggacgttt cgattggcct cggctgatag cgcttcgggc actctctcta
     2581 tcgcaccggg ggcaaaaaga acttttgctt atggtcccaa aagtcccggc ccaaggaaca
     2641 taaagctcaa agaaggaacg gtgctgtacg ggcgggtact ggggcgggta aaaggggaaa
     2701 aatctataat tacacttccg ccagctgggg tcaaccttac ctactaatcg aaagaatgcg
     2761 cgactaaagt cgcgcctccc agagcggggg aggaggctac aaaatcgttt tcagtttcaa
     2821 ctcttttttc ttctctttgt tggttcgctg gaatttgagc gaaaacttgg tgccgtccgg
     2881 gccgcggaag agggcccgca gatcgttcaa ggagtactcc cgcgccggct tgccgttgat
     2941 ctcagtgatt gcgtcgcccc gcttgaatcc tgacttatct cccgcagtac ccttgaccac
     3001 ttcctccacg atgaatttcc ccgccacttc gcggagccag aggcccgatt tgtcgaactc
     3061 gtcgcgctcg ccgaagtgcc ggtttgggaa caggatcagt tgctgtttgg ggtagttaaa
     3121 gatcaccgta aagcgcttta aaagcccgct gccgatgttg ccctggaact tctccgaggc
     3181 aaagatccct ttctgcgccg ttgagagtcc gacgagcagg tcgggaattt caaaagagcc
     3241 cacagtaaaa gacttcatcc gcaccatctg cgtggtggag ggcagatcgt cgccaattcc
     3301 ccctaagcgg gtttcgactc ttttcggata tttgtccgcc agggtgtgtt caaagacgaa
     3361 aggcgagaag aggtcgagcg tattgcgcga gccggtatcg acgttgaacc agccgcggta
     3421 ctgcccctca actgtagcct ccacgtgcgg aatgcgctgg tcaagcgtca taggcagtac
     3481 ctgtccagtg cccgtgtagg tgaagaggtc ggggtcggag aaggtcaatt ggctcttctc
     3541 gtaatcgact tccaccacaa cccgggccag tatgtcgtat cccaagatcc catcaatgcc
     3601 gtggccttcg aattgcgtca acggctgtaa atcgagagtc gccgccacca gactgtcgaa
     3661 ggtcacgtcg ccgacggtaa gtgagccgat cttcaccaag cccgcctttt ccgagccgga
     3721 aacacctttg gcttccagcc cgccggcacc cacgagtccg gacttggcgg caaaggtgct
     3781 atccagcacc accgctccgg cgccggtgtc caaaaggaac gagccggtca cgctcccctg
     3841 aagggtggcg gaaaaataga tgtggttgtt caccagttca aagggtacag tcgtggaggt
     3901 cttgcccggt gcgaaagcaa aatcgcgccg ggctgccgcg ccggggcggg caaagaccgc
     3961 cgggtcaatg gcgacgtttg tttccacgct ggtaatccgg atatcggtgt cgaagatcac
     4021 ctgtccgctg gactggtgca ggtggaacgg ctgcaggagg ccgtccacgc tgcggaagtc
     4081 ggcgtaggtc gtggtgatga gcgcgccatc ttggatttgc tgcgacttgt ccacgagata
     4141 agtccgtcgg ttgataaaca gggtgcggcc caggccgcct tgcggtttca cggctatggc
     4201 gtagaactgg ctgtccgact tttccggcag gaagcggagg gtgaaagtag cggtgtcttc
     4261 ggtgaggtat ttgtatgcag aaaaaaatga ttcggtgacg aactcctctt tctctttctc
     4321 gcccagatcg cgcacgatgc cgttctggtc tttaatccac cagttcgtcc cgtcgcagcc
     4381 caccgacaga ttgaagatcc ccagatcgat atcctggcgg aagcggcccg gcggcgcctc
     4441 gtacgcttcg ctttttccgg taaagccgcc cgcggtgacg gtggcttgag cgtggctggt
     4501 ggtgagggcg cggacctttt ccaatccccc cagcgcctgc gcgtgtttgg ccagaatcga
     4561 ttcgatttcg tttgcatttc cggttcctgg agaaataaac cagatcgaga aaaaccacac
     4621 cgcccacaaa atgagtcggt gggttgcccg caccggcaac tggagagaat agtttttgcg
     4681 tcccataagg taaaagcata gcattccctt cagggccggt caagatactg gcccccaaaa
     4741 tgatggaaaa gtccgttcca acagtggtaa gcatttttcg tataaggcat aaatacgcaa
     4801 ccatgatcaa tatcacataa aggaggtgag tgaagcgtga agaaaggact cgtattcgcc
     4861 ggcctgctgg ttttggcgtt cgtgttcctc ttctcgtctg cgttcgcgca gatgtcccag
     4921 cccaagaagg aagaacccaa gaaggaagcc atgaagatgg aaatggggcc gaaggagatg
     4981 acggtgaccg gcgaagtggt ggacctggcc tgctacatca agatgggcgc ccggggagcc
     5041 gggcacaagg agtgcgccac cgaatgtgcc aacgccggga ttccattggg catcgtcgac
     5101 gccaagggag aaatcttcct gcccgcatcc aaggaggata agaaaggcgc caacgcggaa
     5161 ctgctacctt ttgtggctga gatggtgacg gtgaaaggga agttgtacca caagggtggc
     5221 cagcgcctgc tcgccatcga atcggtggag aaggccgccg ccgcgccggc caagtaagcg
     5281 cgtcacagcc aaagccccgc tggtggtcgg aacgattact accgagcggg gcttttgtgg
     5341 gtcagcggag ggagcaatgt gggttcttgc gtcaaagtta agaaccattt ccggaacacc
     5401 tgaatccgag ctatgaattc agaatccgct cccgccaaac cgggctcggc ccagccggag
     5461 tggttttgtc ccggaaaaaa cttgacttta gaatcagcga acaaatgtac gttttggatg
     5521 aggataaaat cgtctgatgc gagataataa gagcgttatt agaacaccca taacccaaag
     5581 aaggaggttt tcatgaagag aatcttaact cccgtactgg cgttcgtcgc agtctgcgca
     5641 ttcaccgtga tggtgtttgc ccagaccccg gcggccgaca gccagaaggc cgctcccgcc
     5701 accaaagccc aaaccaaggc gcccgccatg aaggcgacca ccaagcctgc cgcggcaaag
     5761 agtctcactt tctctggccg ggtggtttcg gtggatacgg tggctatgac cgtcgtggtc
     5821 aagggcaaaa aggcggaaga aactttcaac gtcgagcccg gcaccaaagt caaggtggcg
     5881 ggaaagacgg cgaagttggc ggacctcaaa aaggacgcct gggtaaaggt tatgtacaag
     5941 atgaaagatg gccggaggac cgccacagcg atcatgtaat tgaacacccg gcttgcgcag
     6001 taaagccaag agccagtcac cactttacta aaagtggcgg ttagggctcg agggggagaa
     6061 tattttctta atcagactcg gcggtgtccg atccccgccg cagtgagagt tctattttgt
     6121 tgtctccgtt cgaccggatt tgactggtca gagaacgacc tgccggaaaa tgaggagtaa
     6181 aacgatgccg accgaaaccg ctctggccga aggcgctccc gccccgcagt tcacgctctc
     6241 cgctgatacg ggccaaaaga tttctttggc cgactttgcc ggcaaaacga acgtcgttct
     6301 ctacttctac cccaaagacg atacccccgg ctgtaccaag gaggcctgtt cattccgcga
     6361 cctgaaaaaa gagtttgaaa aagccggcgc ggcgatctta ggagtctcga tggattcggt
     6421 cgactcccat aagaagttca aagccaagtt caaactgact tttccgcttt tagccgacga
     6481 agacgggaag gtgaccaggg cctacggggt ttacaaagag aagaatatgt acggcaaaaa
     6541 gtactggggg attgagcgga ccacctttgt catcaacaag cagggcaaaa tcg
//
LOCUS       DBNGZD010000151         2220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_234472, whole genome shotgun sequence.
ACCESSION   DBNGZD010000151 DBNGZD010000000
VERSION     DBNGZD010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_234472"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="AC16GM_04155"
     CDS             <1..341
                     /locus_tag="AC16GM_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2922903.1"
                     /translation="ELRRRKKMKFLDIFELAQSDGDLRWLSPAPGGWKQALEKAIAHL
                     PVKYRTCFLLRDVDGWSHEEIAQMLAVPVGTVKSRVARAHHLLQKELKPRLEEINALS
                     HGTPLPVDLR"
     gene            298..1005
                     /locus_tag="AC16GM_04160"
     CDS             298..1005
                     /locus_tag="AC16GM_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922904.1"
                     /translation="MRCRTARRYLLISAEGGTGSAQVTAHAAVCPACRAYAASLAHLR
                     HSAAGIDPQTVPDGFTDRLLVRLRETPKREVQLVPERSRAIAWRWLPVPIALALVAAG
                     GIYLGSGHRTKMRSGPTIPPVAIAPQAAQLPLPPRAVAPDKPSAAENKAELPAPPQRQ
                     LASSRLEESPPTQISERSAPDLSGMIPDGFWFISARDQKNYLMPGVDLNARRLREAQP
                     VYVLPVVATERYVYRDY"
     gene            1048..>2220
                     /locus_tag="AC16GM_04165"
     CDS             1048..>2220
                     /locus_tag="AC16GM_04165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR2922905.1"
                     /translation="MSKLFWIAAGLFFGFIWTNIVTLPEAEAQRTLSNLEEDINALLE
                     GVKPAIVTVEAKHTWEGEPGAKKKKIESEASHRFIGSGIIYNDKGYILTTPNVLQSGP
                     NVLQSGSFQLLFGDQPARSQEKYQVTLGDGRSFSAELIGQDEETNLAVLKVEAANLHP
                     ARLGNSGLLKEGSWITVVGNAYGVPAGVSFGLVNGFRDDGTIQMSVNVSPGSSGGPVL
                     NTKGEVVGLVLAKVSEPFFISLSGDDNKKSTTVKRYSEQLDLPTSGVSLAVPIDRVRE
                     VADQIIAKGKVERAYAGVYPLDLDDDTKENFDLKAGALISEVVAGGPADKAGIKEGDV
                     VTALDGEAVKNEADFRQKMSRLKPDTKATFTVRRKVSGREDQTKEIAVTLGSRSAAA"
BASE COUNT          470 a          636 c          711 g          403 t
ORIGIN      
        1 atgaattgag gcggcggaag aaaatgaagt ttttggacat cttcgaactg gcccaatccg
       61 atggtgacct gcgctggctt tcgcctgcgc ccgggggctg gaagcaggcc ctggagaaag
      121 cgatagccca cctgccggtg aagtaccgca cctgttttct cctgcgagac gtggatggct
      181 ggtcccacga ggaaatcgcg cagatgcttg cagtccccgt gggaacggtg aaatcgcggg
      241 tcgcccgcgc gcaccatttg ctgcaaaaag agttgaaacc aaggttggag gaaatcaatg
      301 cgctgtcgca cggcacgccg ttacctgttg atctccgctg agggaggaac tggttcggct
      361 caggttacgg cgcacgcagc cgtctgtccg gcctgccgcg cctacgcggc ctctttagct
      421 cacctccgcc actcggcagc cgggatcgac ccgcagaccg tcccggatgg cttcaccgac
      481 cgcttgctgg tccggttgcg agagacgccg aagcgggaag tgcaactggt gccggagcga
      541 tccagagcaa ttgcctggcg ttggctgccg gttccgattg ccctggctct ggtggctgcg
      601 ggtggcatct atctggggag cggccaccgg acaaaaatga gaagcggccc gaccatcccg
      661 ccggtcgcaa tcgcccccca ggcggcacag ctgccactgc cgccccgggc agttgcgccg
      721 gataaacctt ccgctgcaga gaataaagct gagcttccgg ccccccctca gcgtcagctg
      781 gcttcgtccc gcttggaaga aagtccgcca acccagatct cagagcggtc ggcgcctgat
      841 ctgtccggga tgattccgga cggcttctgg ttcatctctg ctcgggatca aaagaactac
      901 ctgatgccgg gagtcgattt gaacgcccga aggctacgcg aagcccagcc ggtgtacgta
      961 ttgccggtcg tggccactga aagatacgtt taccgtgatt actgagaagg gaatgaaggt
     1021 accagtgcga aaggaggagg aatgtcaatg agcaaactat tctggatcgc tgcaggtctg
     1081 ttcttcgggt tcatttggac gaacatcgtc acgctgcctg aggcggaggc ccaacggact
     1141 ctttccaatc tcgaagagga catcaacgcc ctcttagagg gggtcaagcc ggccatcgtc
     1201 acggtggaag ccaaacacac ctgggagggc gaaccgggag caaagaagaa gaaaatcgaa
     1261 tccgaggcca gccaccgctt catcggctcc gggatcatct acaacgacaa gggctacatc
     1321 ctgaccaccc ctaacgtgct tcagtccggc cccaacgtgc tgcagtccgg ttccttccaa
     1381 cttcttttcg gcgatcagcc ggcacgctcg caggaaaagt accaggtgac cctgggggac
     1441 ggccggtcgt tttcggcgga actgatcggc caggacgagg aaactaatct ggcggtcttg
     1501 aaagtcgagg ccgccaacct gcaccccgcg cggctgggga attctggcct cttgaaggaa
     1561 gggtcctgga tcactgttgt gggaaatgcg tatggcgttc cggcgggggt gagtttcggg
     1621 ctcgtgaacg gttttcggga tgacggcacg attcagatgt cggtaaacgt gtcccccggc
     1681 tcttccggcg ggccggtttt gaacacgaag ggggaagtgg tcggcctggt gctggcgaag
     1741 gtcagcgagc cgtttttcat ctctctttcc ggcgacgata acaagaagag caccacggtc
     1801 aaacgctata gcgagcagct ggatctgccg acttcggggg tcagcctggc ggtaccgatt
     1861 gaccgcgtcc gggaagtggc cgaccagatc atcgccaagg gaaaagtgga gcgcgcctac
     1921 gccggcgttt atcccctgga cttggacgat gacacaaaag agaacttcga tctcaaagcc
     1981 ggggcgctga tctcggaggt ggtggcggga gggccggcag acaaggcggg catcaaagag
     2041 ggtgatgtgg tcacggccct cgatggcgag gcggtgaaaa acgaagccga cttcaggcag
     2101 aaaatgagcc gcctgaaacc ggatacgaag gcgaccttca cggtcagacg aaaagtctcc
     2161 ggacgggaag accagacgaa agagattgcc gtgaccctgg gcagccgctc cgcggccgcg
//
LOCUS       DBNGZD010000152         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_372959, whole genome shotgun sequence.
ACCESSION   DBNGZD010000152 DBNGZD010000000
VERSION     DBNGZD010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_372959"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="AC16GM_04170"
     CDS             <1..1590
                     /locus_tag="AC16GM_04170"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR2922906.1"
                     /translation="GGKTPLTTSSGIPIKTLYTPEDKEHLESPAKLGQPGQFPYTRGI
                     YPDMYRGKLWTMRQYAGFGTARESNERYKYLLSQGQTGLSVAFDLPTQIGYDPDHPLA
                     NGEVGKAGVSIASLVDMETLLAGLPLDQISTSMTINATAIILLALYVAVARKRKIPLE
                     KLAGTVQNDILKEFIARGTYIFPPKPSLRLVTSIFLYAKDRLPRWNTISISGYHIREA
                     GSTAVQEVAFTLADAVAYVQTALEAGLAVDDFAPRLSFFFNAYNDFLEEVAKFRAARR
                     IWAHLMREKFKAQKPDSWKLRFHTQTAGSTLTAQQPENNIVRTTLQALAAVLGGTQSL
                     HTNAYDEALALPTEKSVQIALRTQQIIAYESNVAATADPLGGSYYLEALTDAIETGVW
                     KYLEQIEKAGGTLACVESGFFQREIQRSAFAYQKKVEAGERVVVGVNRFTAKEAEQKN
                     ILKVDLRVQEEQMKSVRRLRQERDSSKTEKARARLLAEAKGSSPLFEPILECVENYVT
                     LGEICETLRSVWGEFRQQVIL"
     gene            1604..2002
                     /gene="mce"
                     /locus_tag="AC16GM_04175"
     CDS             1604..2002
                     /gene="mce"
                     /locus_tag="AC16GM_04175"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR2922907.1"
                     /translation="MFTRLDHIGIAVRDLDKAVAYWSELLELKSDHRIPVPAQGVEVV
                     FIPTRGARLELLAATGPDSPIAKFLERRGEGLHHVCFAVRDIEAELADLKSKGVELID
                     EKPRLGAEGKRIAFLHPKSTYGVLIELLEE"
     gene            2095..>2820
                     /locus_tag="AC16GM_04180"
     CDS             2095..>2820
                     /locus_tag="AC16GM_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR2922908.1"
                     /translation="MPKGMRNVYMVSGGVSKFEKARPDVTFQPMVKEAYDYALKDLGL
                     EFKEASEVVDWTVASYFSDHFMRQLKAGAMVQDYLGLCPKPSHRVEGGGATGGLAFQE
                     AWKTVASGLAEVCVTYGWETMSHVNTWKGNEFIALASDTSFDYPVGGFYSGYYAMMVV
                     RHMHEFGTTVEQLAKIAIKNHKHAYYNPYSQKKIFDLSIKDVRNSPMVAYPLTRYDIC
                     VMSDGAAVAFLASEEGIKRIEKKT"
BASE COUNT          640 a          794 c          824 g          562 t
ORIGIN      
        1 ggcggcaaaa cgccgcttac gaccagttct ggaattccca tcaaaactct ctacacgccc
       61 gaagacaaag agcatctgga gagtcccgcg aaactcggcc agccggggca gtttccctac
      121 acgcggggga tataccccga tatgtaccgg ggcaaactct ggacgatgcg ccagtacgcc
      181 gggttcggca cggcccgcga gtccaacgag cgctataagt accttctctc acaggggcag
      241 accggactct cagtggcgtt tgatttgccc acgcagatcg gttacgaccc ggatcatccg
      301 ttagccaacg gggaagtggg caaagccgga gtctccattg ccagtttagt ggacatggag
      361 acgcttctgg ctggtttgcc cctggatcaa atttccacct ctatgaccat caatgcgacg
      421 gctatcatcc ttctcgcgct ctacgtcgcc gtggccagga aaagaaaaat tcctttagaa
      481 aaactcgccg ggacggttca gaatgacatc ctgaaagaat ttattgcccg cggcacctac
      541 atttttccgc ccaagccatc cctccgcctc gtcacctcaa ttttcctcta tgccaaagac
      601 cgcctgccgc gctggaacac gatttcgatc tccggctacc atatccgcga ggcggggtct
      661 acggcagtgc aggaagtggc cttcacgctg gctgatgcgg tggcctacgt tcaaacggcg
      721 ctggaggccg gcctggcggt tgacgatttc gcgccgcggc tctcgttttt cttcaatgcg
      781 tacaacgact ttctggagga agtggccaag ttccgcgccg cgcggcggat ctgggcgcac
      841 ctgatgcggg aaaagttcaa agcgcaaaaa cccgattcct ggaaattgcg ctttcacacg
      901 cagaccgccg ggtcgacctt aaccgcccag cagccggaga acaatatcgt ccgcaccacg
      961 ttgcaggctt tggctgcggt cttgggcgga acgcagtcgc tccacaccaa cgcctacgac
     1021 gaagcgctgg ccctgccgac ggaaaaatca gtccagatcg cgctccgcac ccagcagatc
     1081 atcgcctacg aatccaatgt cgccgccacg gccgaccctt tgggcgggtc gtactacctt
     1141 gaagcgctca ccgatgcgat tgagaccggc gtctggaagt acctcgagca aatcgaaaaa
     1201 gcgggcggga ccttggcctg cgtggagtcg ggttttttcc agagggaaat tcagcggagc
     1261 gcctttgcct atcagaagaa agtcgaagcg ggggagcggg tcgtggtggg ggtaaatcgc
     1321 ttcaccgcaa aagaggcgga acaaaaaaat attcttaagg ttgacctgcg ggtgcaggag
     1381 gagcaaatga aaagtgtccg ccggttgcgc caggagcgcg atagcagcaa gaccgaaaaa
     1441 gcccgcgccc gcctgctggc tgaagccaaa ggaagcagtc ccctctttga accaattctg
     1501 gaatgcgtgg aaaattacgt gacgttaggc gagatttgcg aaacgctccg cagcgtgtgg
     1561 ggggaattcc ggcagcaggt gatcctctga gtccgagtta aacttgttca cccgtctcga
     1621 tcacattggc attgccgtcc gtgacctgga caaagcggtt gcttactggt cagagctcct
     1681 tgaactgaaa tctgaccaca gaatccccgt tccggcgcag ggtgtggagg tggttttcat
     1741 tcctacgcgt ggagcgcggc ttgagctttt ggccgcaacc ggaccggact cccccattgc
     1801 aaaatttctg gagaggcgcg gcgaggggct gcaccacgtt tgttttgcgg tgagggatat
     1861 cgaagcggag ttggcggact taaaatcgaa gggggttgaa ctaatagacg aaaagccgcg
     1921 ccttggggct gaaggaaaaa gaattgcctt cctgcaccca aagagtactt acggagtctt
     1981 gattgagtta ctcgaagagt gaagctggcg gagcaagtta ggcaaaccaa cctgcgctac
     2041 cgtttcgcca aaatacaaaa tctattatct tcggccggaa caacaggagg attcatgcct
     2101 aagggaatgc gcaacgtcta catggtttcc ggcggggttt cgaagttcga aaaagcccgg
     2161 cccgacgtca cctttcagcc gatggtcaaa gaggcctacg actacgcctt gaaggacctg
     2221 gggctggagt tcaaagaggc gagcgaggtc gtcgattgga ccgtcgcctc ctacttctcc
     2281 gaccacttta tgcgccagtt aaaggccggg gcgatggtgc aggattactt aggactctgc
     2341 cccaagccgt cgcaccgcgt ggaaggcggc ggcgccacgg gtgggctggc gttccaggag
     2401 gcctggaaga cggtcgcttc cggtttggcc gaggtgtgcg tcacctacgg ctgggagacg
     2461 atgtcccacg tcaacacctg gaaggggaat gagttcatcg cgctcgcctc cgatacgtct
     2521 ttcgattacc ccgtgggggg cttctactcc ggctactacg cgatgatggt ggtgcggcac
     2581 atgcacgagt tcggcacaac cgtcgaacag ttggccaaga ttgccatcaa aaaccacaag
     2641 catgcctact acaaccccta ctcgcagaaa aagatttttg atttgagcat caaagacgtg
     2701 cgtaattcgc cgatggtggc ctacccgctg acccgctacg acatctgcgt gatgtccgac
     2761 ggagccgcgg tcgccttctt ggccagtgag gaggggatca agcgcatcga gaagaagacc
//
LOCUS       DBNGZD010000153         1728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_512806, whole genome shotgun sequence.
ACCESSION   DBNGZD010000153 DBNGZD010000000
VERSION     DBNGZD010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_512806"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..>1728
                     /locus_tag="AC16GM_04185"
     CDS             305..>1728
                     /locus_tag="AC16GM_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR2922909.1"
                     /translation="MTSNIKEAIRLEEVRDGKALWKKWGPYLSERQWGTVREDYSVGG
                     DAWDYFTHDQARSRTYRWGEDGIAGLSDDKQRLCFALALWNGNDPILKERLFGLTNSE
                     GNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQAAFHYSDLVETNRRRSRAEMEYELLDT
                     GVFDGDRYFDVFVEYAKGGAEDILVQITAINRGPEEAEVHLLPTLWFRNDWAAWIARP
                     AEKPNLKQIEGRAGTCAVAAAHPVVGEYTLYCEGEVPLLFTENETNYERVFGTPNEAP
                     YVKDGINNYVVLGQQGAVNSEKTGTKVAPHYRFTIGPGQSATVRLRLTAQAPAGESQK
                     TTAAATPFGTEFEQTLTARLQEADEFYQSVTPPSVSPDAANVMRQAIAGMLWSMQFYF
                     FDGDHWLDEHRANPLHPGSRDFRNREWFHMVNEDIISMPDKWEYPWYAAWDLAFHTLP
                     LAIVDPDFAKQQLELMLRGVYLHP"
BASE COUNT          379 a          547 c          479 g          323 t
ORIGIN      
        1 ctttgacagg atggacccgt acgtacggtt cgtatttttt ttgggagttc agccgatggt
       61 aatccaggta aaggtcaatt gccttggcta ctttcatgct ccacctcctg gtttgattgc
      121 atcgtgccga tgcggatagc caaaccatat caaattattt ccagcgaatc tgataacttt
      181 cagctgtggc aagttatcaa ttatacgcca ccctttcgga aggctttttc caacatccat
      241 ttcaagcttc attccccatt cttaaactta atcctgaaca gagattctca aaaaaggagt
      301 tggtatgacc tccaatataa aagaggctat ccgcctggaa gaggtccggg acggcaaagc
      361 gctctggaag aagtggggcc cgtacctgag cgaacggcaa tggggcacgg tgcgggagga
      421 ttacagcgtg ggtggcgatg cctgggacta cttcacccac gatcaggccc gctcgcggac
      481 ctaccgctgg ggcgaagatg gcatcgccgg cctttccgac gacaagcagc ggctctgctt
      541 cgccctggcc ctttggaacg gcaatgaccc cattctcaag gaacgcctct tcggcttgac
      601 caacagcgag ggcaaccacg gcgaggacgt caaagaatac tatttctacc tcgacagcac
      661 ccccacccac tcctacatga aatatctcta caagtatccc caggcggctt tccattactc
      721 cgacctggtg gagacgaatc gccggcgcag ccgcgccgag atggagtatg aactcctcga
      781 caccggcgtc ttcgacggcg accgctattt tgacgtcttc gtggagtacg ccaaaggcgg
      841 tgcagaagac attctggtgc aaatcaccgc catcaaccgg gggccggaag aggccgaagt
      901 gcacctgctg cccaccctgt ggttccgcaa cgactgggcg gcgtggattg ccagacctgc
      961 cgagaaaccg aacctcaagc agatcgaggg acgggcgggc acgtgtgcgg ttgcggcggc
     1021 gcacccggta gtcggtgagt acaccctgta ctgcgagggc gaggtgccac tactcttcac
     1081 cgagaacgaa accaactacg agcgcgtttt cggcacgccc aacgaggccc cgtacgtcaa
     1141 ggacggcatc aacaactacg tagtgctggg ccaacagggc gcggtgaact ccgagaaaac
     1201 tggaacaaag gtggcccccc actaccggtt cacgatcggc cccggccaat cggcgacggt
     1261 gcggctgcgt ctcaccgccc aggcccctgc cggggaaagc cagaaaacca cagccgccgc
     1321 aacccccttc ggcacggagt ttgagcagac cctgaccgcc cggctgcaag aggcagacga
     1381 gttctaccag tccgtgactc cgccctcggt ctccccggat gcggccaacg tgatgcgcca
     1441 ggccatcgcc ggcatgctct ggagcatgca gttctacttc ttcgacggtg accactggct
     1501 ggacgagcac cgcgccaacc ctctccatcc cgggagccgc gacttccgga accgggagtg
     1561 gttccacatg gtgaacgagg acatcatctc catgcccgac aagtgggagt acccctggta
     1621 cgcggcctgg gacctcgctt tccacacgct gcccctcgcc atcgtggacc ccgacttcgc
     1681 caagcagcag ctggaactaa tgctccgcgg ggtctacctg caccccag
//
LOCUS       DBNGZD010000154         3586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_373187, whole genome shotgun sequence.
ACCESSION   DBNGZD010000154 DBNGZD010000000
VERSION     DBNGZD010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_373187"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2882
                     /locus_tag="AC16GM_04190"
     CDS             <1..2882
                     /locus_tag="AC16GM_04190"
                     /inference="COORDINATES: protein motif:HMM:NF048764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF7450 family protein"
                     /protein_id="HIR2922910.1"
                     /translation="LATVSDPGAPRSMGQTIGQPDPSPPDYPANSFFDVFVEVSLEHA
                     PWLGGQPDVIYTGNPGNQYQPVRVTDPKLGCIPPPPNDSFCTSPGFSVLLTSRRTNQV
                     VGQLISVWHVVLPPNHYKTWKITDFSPPQIRQVIAKDQFMVDNLVLDSAVYLSNPAQK
                     CVPGRCFNIADTTDHFTWYQARGRETLLDVDFTNQFQQSTVRIDSVKYFLLPTHKFPH
                     NPPRLLDHYKAYKIRNPQQITVPVQLLDQFDLSGPENINLLIPRYFLTPAQKNTEPVY
                     DTLTHFVAYEINPKRLSTLSRDIEDQFGIHPIRIDSSVFVLVPTCKLRAQPPPPEEAC
                     CLPNGTCQLVPVGQCAAIGGTVVPACLGDGNFNGKDDACEVPPPRDSSQNHYKTWRVV
                     EQGPVVPRTVSVLDQIMRDDNVRLDSIQYLSNPVQKCIEMQPGFGICFDIVDTTDHLT
                     WYRAFGRDTLLQIVFQNQFTDPFNQNVRDSVLIDSVRYLLLPTRKFPHQPPVKLDHYK
                     AYRIRNPRPVPLPGFGTTLLDQFDLVDPRGTEFIVPNEVVPRYFLTPARKDVGGVIGQ
                     RFDTLTHYVAYEFNPKRPTALTRDIEDQFGIHILQVDSSVLILVPTKKLKVEPPPPQG
                     GCCLPDGKCVALDLQGCLAVGGTHVPACLGDLNGNGKDDACERPEPCDSLREELIINT
                     TRPHQLHPGDTVTVCIKITNLFNNRVCCLDSVIFEQAGFGIVVPGWNVINKLTFVPCR
                     PIPPGGTLTLCVLDTIPGTGHGCIRVRKYSDCCNDTLQQNLDAEDDSDCGPKCDTFRV
                     DIPSNPQGLPYNLVSICNVPTGWTCTVTPANLPPVGGLVTIIICTDTIRYTPGDTIPK
                     DSGCVTVVALAGGVIAGEATFCKYISYMHGDVAGDPVCPDGVIDIVDIVGLINNVVYG
                     ADYCAPMPAGDVAGDAQNCPDGIVDIVDIVGLINNVVFSNPLCCK"
     gene            3038..>3586
                     /locus_tag="AC16GM_04195"
     CDS             3038..>3586
                     /locus_tag="AC16GM_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR2922911.1"
                     /translation="MKRGWLAGLVIAGVILFFVLLTVAYFWTTYNKLVTRNEAIKNAW
                     AQVDNQLERRSDLIPNLVETVKGYATHERELFEKIADARARLAGARSPDEKMTVSNEL
                     SGYLGRLLVVVENYPNLKANESFNNLMFELSGTENRIAVERKRYNDTVLGYNQSLRRF
                     PTNFVASMFRFSQAPYFQVEEGK"
BASE COUNT          758 a         1038 c         1027 g          763 t
ORIGIN      
        1 gtttggccac ggtgagtgac ccgggcgccc cgcgctctat gggtcaaacg attggacagc
       61 cggacccgag cccccctgat tacccggcca attccttttt tgatgttttt gtcgaagtat
      121 cgctggagca cgcgccgtgg cttggcgggc aaccggatgt aatttacacc ggcaatcccg
      181 gcaaccagta tcagcccgtg cgggtgactg atcccaagtt gggttgcatt ccaccgccgc
      241 cgaacgacag cttttgcacg tcacccggct ttagcgtcct gttgaccagc cgccggacca
      301 atcaagttgt tggccaactg atttcggtgt ggcacgtcgt cctaccgccg aatcactaca
      361 aaacctggaa gattaccgac ttttccccgc ctcaaatccg gcaggtgatt gcgaaagacc
      421 aatttatggt ggataatctg gtcttggaca gcgcggtcta cctctccaac ccggcccaga
      481 agtgcgtccc cggtcgctgc tttaatatcg ccgacaccac cgaccatttt acctggtatc
      541 aggcgagagg cagggaaaca ctcttggatg ttgactttac gaatcagttt cagcaaagca
      601 ccgtccgcat agattcggtg aagtatttcc tgcttcctac ccataagttt ccccataatc
      661 cgcctcggct gctggaccac tacaaggcct acaagattag gaatccgcag cagattacag
      721 tgccggttca gttgctggac cagttcgacc tcagcggacc ggagaatata aacctgctga
      781 ttccacgcta tttcttaact ccggcccaaa aaaataccga gccggtgtat gacacgctga
      841 cccactttgt ggcctacgaa ataaatccga aacggctcag tacgctgagt cgggatattg
      901 aggaccagtt tggcatccat ccgattcgaa tcgacagcag cgtgttcgtt ctggtgccta
      961 cttgtaagct gagggcgcag ccgcctcctc cagaagaggc ctgctgtctg cccaatggga
     1021 cgtgtcagct ggtgccggtg ggccaatgcg cggctattgg cggtacggtc gttccggctt
     1081 gtttgggtga cggcaacttt aacggcaaag acgatgcctg cgaagtgccg ccgcctcgag
     1141 actcgtcgca gaaccactac aaaacctggc gggttgtgga gcaaggcccg gttgttcccc
     1201 ggacggtgag tgtgctggac cagattatgc gggatgataa cgtcagactg gacagcatac
     1261 agtacctttc caacccggtc cagaaatgca tcgaaatgca acccggtttc gggatctgct
     1321 tcgatatcgt cgatacgacc gaccatctga cctggtacag agcgtttggc cgggacactc
     1381 tgcttcagat agtgtttcag aatcagttca ccgatccgtt caatcagaat gtgcgtgatt
     1441 cggttctcat agactcggtg cggtatttgc tgcttcccac ccggaagttt ccgcatcagc
     1501 cgccggtgaa gctggaccac tacaaggcct accggatcag gaatccgcgg ccggttccgc
     1561 tgccggggtt tggaaccact ctgctcgacc agttcgacct cgtcgatccg cgcgggacgg
     1621 agttcatagt cccgaacgaa gtggtgccgc gctacttttt gaccccggcc cggaaggatg
     1681 tcggcggggt tatcggccag cggtttgaca cgttgaccca ttacgtggct tacgaattca
     1741 atcccaaacg gcccactgcc ctgactcggg atatagagga ccagtttggt atccatattt
     1801 tgcaggttga cagcagcgtg ctcattctgg tacctacgaa aaagttgaag gtggaaccgc
     1861 caccgccgca agggggctgc tgtctccccg acggcaagtg cgtcgctctc gatctgcagg
     1921 ggtgcctggc ggtgggcggc acacacgttc ctgcctgctt gggtgacctg aacggcaacg
     1981 gcaaagacga cgcctgcgag cggcccgaac cctgcgacag tctgagagag gagttgatta
     2041 tcaacacgac gcggccgcac cagctccatc cgggcgacac ggtgacggtc tgcatcaaga
     2101 tcaccaacct cttcaataac cgggtctgct gtctcgattc ggtcatcttt gagcaggccg
     2161 gtttcggcat cgtggttccc ggctggaacg tcatcaacaa gttaaccttc gtaccctgcc
     2221 ggccgattcc gcccggcgga acgctcaccc tctgcgtcct cgacaccatc ccgggaacgg
     2281 gtcacggctg catccgggtg cggaagtact ccgattgctg caacgatacc ttgcagcaga
     2341 acctggacgc cgaggacgat tccgactgcg gacccaagtg cgacaccttc agggtcgaca
     2401 ttccgagcaa tccgcagggg ctgccgtaca acttggtcag catctgcaac gtgccgaccg
     2461 ggtggacctg cacggttact ccggcgaatc tgccgccggt gggcggtctg gtgaccatca
     2521 tcatctgcac cgacaccatc cgctacaccc ccggagacac cattcccaag gacagcggct
     2581 gcgtgacggt cgtagccctg gctggcgggg tgatcgcggg cgaggccacc ttctgcaagt
     2641 acatctccta catgcacggt gacgtggcgg gtgacccggt ctgcccggat ggagtgatcg
     2701 acattgtgga catcgtcggt ctgatcaaca acgtggtgta tggtgccgac tactgtgcgc
     2761 cgatgccggc cggggatgtg gcgggtgatg cacagaactg tccggacgga atcgtcgaca
     2821 ttgtggacat cgtcggcctg atcaataacg tggtcttcag taatccgctc tgctgcaagt
     2881 agagcagggc gtacctcctt accgaaagga gtgagtgcca ggcggagcgg cgcaagccgc
     2941 tccgcttctt ttttcctgca gcgacaggag tgaccggact tgacggacga aaggacaggc
     3001 atattttgat atgtatggag ggagaaagcg gatgcccatg aaacgcggct ggctggctgg
     3061 cctggtaatc gccggtgtca tattattttt tgttcttttg accgtcgcct acttctggac
     3121 gacctacaac aaactggtca cccgaaacga agcgataaag aacgcctggg cgcaggtgga
     3181 caatcaactg gagcggcgct cggacctgat tccgaactta gtggaaacgg tcaagggata
     3241 tgcgacgcat gaacgggaac tcttcgagaa gattgccgac gcccgggcgc ggctggctgg
     3301 cgcccgcagc ccggacgaga agatgaccgt ttccaatgaa ctttccggct acttaggacg
     3361 gcttttagtc gtggtggaga actacccgaa cctgaaggcc aacgagagtt ttaacaatct
     3421 gatgttcgaa ctctccggga ccgaaaaccg gattgccgtc gagcgcaagc gctacaacga
     3481 tacggtgctg gggtacaacc aatccctgcg ccgcttcccc acgaatttcg tagcatctat
     3541 gtttcggttc agtcaggcgc cctatttcca ggtggaggag gggaaa
//
LOCUS       DBNGZD010000155         1499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1832036, whole genome shotgun sequence.
ACCESSION   DBNGZD010000155 DBNGZD010000000
VERSION     DBNGZD010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1832036"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1291)
                     /locus_tag="AC16GM_04200"
     CDS             complement(182..1291)
                     /locus_tag="AC16GM_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922912.1"
                     /translation="MDELDKLFEQNYAQHLLEATQREPKLTAPVDLSRAAQVSVTRDM
                     YVEAQAKGLTLSELLESDEYDPSPLACPLDAFERQLAVKGIKVGGPNPSTVELFFSTA
                     PTLLPEFMLREIKRGQKMRPGLADLGATSTVINANRYTPFFVSGTPTQPKFSLRPIGD
                     GAEVPKLVVAEQLHTITIPDYGMALEVSYKALRHRTTAQFKVLLWYVGFRLQADKMGL
                     VVNTIVNGDGNANPATVLNTAATGVLTYADLITFWAEFFPYELNVVLCHKDKLKSILT
                     LPEFKDPMAGFKFQRTGEVVSPLGSKLIRSDDVPNDLVIGLDSRFAIEEVIAQPVMVE
                     FDKVIKAKLEEAVISESVAYAKVIKEASLVLDTVW"
BASE COUNT          290 a          424 c          434 g          351 t
ORIGIN      
        1 cccaacagta tttcccgctc tacctgaaag agatggagtt ccggtacaac caccgcaaag
       61 agcccatgtt cgaacgattg gcacaactaa tggcaaatcc cgtgtcaaat cttttataat
      121 cacctaaaag atttgtcctt caacttcgac acctcgaccc cccgatactc cgacacagtt
      181 tttaccatac tgtatccagg accagactcg cttctttgat caccttggcg taggcgaccg
      241 attcggagat caccgcttcc tccaacttcg ccttgatcac cttgtcgaac tccaccatta
      301 cgggctgggc gatcacctct tcgatggcaa agcggctgtc cagaccgatg acgagatcgt
      361 tcggcacgtc gtcggaacga atgagtttcg atccgagcgg agagaccact tcgccggtgc
      421 gctggaattt gaagccggcc atcgggtctt tgaactccgg cagggtcaaa atcgatttca
      481 gtttgtcctt gtggcagagg acgacattca gttcgtaggg gaagaattcg gcccagaagg
      541 tgatcaaatc ggcgtaggtc aggacgccgg tggcggcggt gttgagcacc gtcgccgggt
      601 tggcgttgcc gtcgccgttg acgatggtgt tcaccaccag gcccatttta tcggcctgca
      661 gccggaagcc gacgtaccag aggagcactt tgaactgtgc ggtggtgcgg tgccggaggg
      721 ctttgtacga aacttccaag gccatcccgt agtccggaat ggtgatggtg tggagctgct
      781 ccgccacgac cagcttgggc acttccgcgc cgtcaccgat gggtctgagg gagaacttgg
      841 gctgggtcgg tgtgccggag acgaaaaagg gcgtgtagcg gttggcgttg atgacggtag
      901 acgtagcccc gaggtcggcc agtcccggac gcatcttctg cccgcgcttg atttccctga
      961 gcatgaattc cggcaggagg gtcggcgcag tggagaagaa aagttccacc gttgacggat
     1021 tcgggccgcc gactttgatg cctttcaccg ccagttgccg ttcgaaggca tccaggggac
     1081 aggcgagcgg cgagggatcg tactcgtctg attcgaggag ttcggagagg gtcagacctt
     1141 tcgcctgcgc ttctacgtac atgtcgcggg tgacggaaac ctgggcggcg cgcgagagat
     1201 ccaccggcgc agtcaatttc ggttcgcgct gggtggcctc taagagatgc tgcgcgtagt
     1261 tttgttcgaa gagcttatcg agttcgtcca tttcttttcc tttcagttca cttctggggc
     1321 acggcacgcc gtgtccgtac gggttgtctt ccgtctcagt tcatttctgg gcacggcacg
     1381 ccgtgcccgt acgttgacct aagcacagtg ccggagtttt tttgattcgc cattgtcttt
     1441 ccctaccacc taccacccat cacctatccc tcgactttgc tcgtggcaag ccacctttc
//
LOCUS       DBNGZD010000156         2977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_512875, whole genome shotgun sequence.
ACCESSION   DBNGZD010000156 DBNGZD010000000
VERSION     DBNGZD010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_512875"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..868)
                     /locus_tag="AC16GM_04205"
     CDS             complement(53..868)
                     /locus_tag="AC16GM_04205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2922913.1"
                     /translation="MSHDKDYVLGTHDEEIARLALQHRVWRPRALDAWRRAGFTVGQT
                     LLDIGCGPGHASIDLAEIVGPSGRVIAIDRSRRFLDALDAARRQRGLNNIEALELDLD
                     EAELPALGADGAWSRWVFAFVKHPRELLARVAGALKPGGVLVLHEYFDYSTWRLAPRL
                     PELEEFVKVVIESWRADGGEPNIGLDLPMWLAELGFEIKTLNPIIDVVPASNFIWQWP
                     KAFIQVGLRRLVDLGHLTSERAQAIAEAFAAAETAPHTLMITPAVLEIIAVRK"
     gene            complement(907..1449)
                     /locus_tag="AC16GM_04210"
     CDS             complement(907..1449)
                     /locus_tag="AC16GM_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR2922914.1"
                     /translation="MAFLRGINVGGRKIIKMEDLSRMFVSMGFRNVKTYIQSGNVIFE
                     TAEINSNVLTKKIEKELHKSLGYEVRVFLRTILEVEDIVQHNPFKGMAANANVGMYVT
                     FLSEEPKSKPELPLVSPQKDVEVFRIRKREAFILCRKIKGRSGFPNNFIEKELGMPAT
                     TRNWTTISKIIIQTLPKPGR"
     gene            complement(1489..1959)
                     /locus_tag="AC16GM_04215"
     CDS             complement(1489..1959)
                     /locus_tag="AC16GM_04215"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR2922915.1"
                     /translation="MQTIDVTEEHVRQILKDLVFRALWFPLFVFLLHVFLSQVLRAYQ
                     KFPVLGSPMHLLGGIAITFFFSRVLTILQKSGLAGELDRRIRTMLLFALTATAAVFWE
                     FGEFLVDHFFGTRMQLGLEDTLLDMFLGIVGGVSYLSAGLLVSSAGRNKRIART"
     gene            complement(2160..2699)
                     /locus_tag="AC16GM_04220"
     CDS             complement(2160..2699)
                     /locus_tag="AC16GM_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR2922916.1"
                     /translation="MFPSEFKFSTSKYIYSIKGDGQGLRKVNETDTKAFVLVQESQDM
                     LSHLSFLDRGEGVILLFEITNDSLDAGWGEIVEFNQSSLKIYWRKNIPFNVGEPLVED
                     NYVYLTGIGYIAKLALDSGKVIWQHDNLYDPKSYHFNSFEIPKIAGDTVLFMESPDQV
                     KEAKTVRVHRIAGKILSIK"
BASE COUNT          728 a          780 c          711 g          758 t
ORIGIN      
        1 cctaaaggtg catctgatga agttgtgaca agaggtgttc aaatcgtccg tctcacttac
       61 gaacggcgat gatctccagc accgcgggcg taatcataag cgtgtgcggc gcagtctcgg
      121 cggctgcaaa cgcttccgcg attgcctgcg cgcgttcact ggtcagatgg cccagatcaa
      181 ccaggcggcg caggcccact tgaatgaacg cctttggcca ctgccaaata aagttagatg
      241 ccggaacgac gtcgattatg gggttgagag tcttaatctc gaatcccagt tctgcgagcc
      301 acatgggcag gtcgagcccg atgttcggct cacccccatc ggcgcgccag ctctctatca
      361 ccaccttcac gaactcctca agctccggca accgtggcgc aagccgccag gtggagtagt
      421 cgaaatactc gtgcagaacc agtacgccgc ccggtttcaa tgcgcctgcc acccgggcga
      481 gaagctcgcg gggatgcttg acgaacgcaa agacccagcg ggaccaggcg ccgtcggcgc
      541 caagagcggg caattccgct tcgtcgaggt cgagttcaag cgcttcgata ttattaagtc
      601 cgcgttgccg tcgtgcagca tcgagcgcgt ccagaaaccg tctcgacctg tcgatggcga
      661 tgaccctccc ggaaggtccg acgatctctg ctaaatcaat cgaggcgtga ccggggccgc
      721 acccaatatc gaggagcgtt tggcccaccg tgaatccggc ccgccgccag gcatccagcg
      781 cgcgcggccg ccagacgcga tgctgcagcg ccaaccgtgc gatctcctcg tcgtgcgtgc
      841 ccaggacgta gtctttgtcg tggctcatcg ttgctctctt ctccgcagcg gttcgtgaac
      901 tactcactac ctcccgggtt tcggcagagt ttgtattatg attttactga ttgttgtcca
      961 gttccgtgtc gttgccggca ttccaagttc tttctcgatg aagttgttcg gaaaaccgga
     1021 ccttcccttt atcttgcggc ataaaatgaa cgcttcacgt tttcttatgc ggaaaacctc
     1081 aacgtctttt tgaggagata ccaaagggag ttccggcttg ctttttggtt cttcggataa
     1141 aaaagtgacg tacatcccta cattggcgtt ggctgccatt cctttgaaag gattgtgttg
     1201 cacaatgtct tcaacttcaa gaatcgtcct caagaaaact cttacctcat aacccagcga
     1261 tttatggagt tctttctcaa ttttcttggt aagaacgttg gaattgattt ccgcagtttc
     1321 gaaaatgacg ttcccgctct ggatgtaggt tttcacgttt ctgaatccca tcgatacaaa
     1381 catccggctc aagtcttcca tttttattat cttgcggccg ccgacattaa tgccgcgcag
     1441 aaatgccaca tatgtaatca tttttatcgc ctatgcccct tttctatttt acgttcgggc
     1501 tatccgcttg tttctcccgg ctgacgacac caacagcccc gctgataagt aagagacccc
     1561 gcccacaatg ccgaggaaca tgtcgagtaa agtgtcttcc agaccaagct gcatacgagt
     1621 accgaaaaag tgatccacca gaaactcgcc gaattcccag aacaccgccg ccgtcgccgt
     1681 taaggcgaag aggagcatgg ttcggatgcg ccgatcgagt tcccctgcca gaccgctctt
     1741 ctgcagaatc gttagtacac gggagaaaaa gaaggtgatg gcaatgccgc ccaaaaggtg
     1801 catcggggaa ccgaggacag ggaatttttg atacgcgcgg agtacctgcg aaaggaaaac
     1861 gtggaggaga aagacaaaga gtgggaacca gagggcacga aaaaccaggt cttttaaaat
     1921 ttgtcgaacg tgctcttcag tgacatctat cgtctgcaag gtatcctgtc ctcgacccta
     1981 attcgaaacc aaaactcgga tgcacagggc tgatctgcct gacttgtatt taggccgacc
     2041 ggcctaccac catgccatcg atgtatatta tgaacgggta ggtagaatgt aaacgctgtt
     2101 cattttttta aatgagctag gaatctggga caactttaga acttactttc aaactgccat
     2161 cattttatag atagtatttt accagctatg cgatggactc ttaccgtttt tgcttccttt
     2221 acttggtcag ggctttccat aaaaagtact gtatcgcccg ctatcttcgg aatttcaaat
     2281 gaattgaaat ggtagctttt tgggtcgtaa agattatcat gttgccaaat aaccttcccc
     2341 gagtcaagcg ccaacttagc aatataccct ataccagtaa ggtatacata attgtcttcc
     2401 acgagaggtt caccaacgtt gaaggggatg ttttttctcc agtatatttt taaagagctc
     2461 tgattgaact ctacaatttc accccatcca gcgtctaatg aatcattggt aatctcgaat
     2521 aaaaggataa cgccctcgcc cctgtcaaga aaagaaagat ggctgagcat atcttggctt
     2581 tcttgaacga ggacaaatgc ttttgtatca gtttcgttta ctttcctcag accttgaccg
     2641 tcacctttaa ttgaataaat atacttggaa gtggaaaatt tgaattcgga cggaaacatc
     2701 gagtccccaa tagcttctgc cttactaaaa gtcttattgg gtgattcgga gcgtttcgaa
     2761 gtacagcccc caaaaatggc aattgcgaat aactgcaacc aaaaaactcg ttgaaaggag
     2821 gctcgcgcta atgccctttt ggctttatac cgcatttgca cctcgttatc gaatcaagcg
     2881 gagaggctgg gattcgccgt caggccctga gcgagtcctt tggacgagtc gaagggaacc
     2941 catttatgag tcgttccttt ttctcacgac gccatcc
//
LOCUS       DBNGZD010000157         1318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_582176, whole genome shotgun sequence.
ACCESSION   DBNGZD010000157 DBNGZD010000000
VERSION     DBNGZD010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_582176"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="radA"
                     /locus_tag="AC16GM_04225"
     CDS             complement(<1..925)
                     /gene="radA"
                     /locus_tag="AC16GM_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR2922917.1"
                     /translation="MQPAKLKTIYACQSCGAQQSRWLGKCPECGTWNSLVEEKISLAP
                     TKRGRGEKVASEKPVALSTIEPLERERYLSGLDEFDRILGGGAVDGSTILVGGDPGIG
                     KSTLLLQVGAAVAKRYGGVLYISGEESHRQIKMRAERLGLEGLGALAILTETDAQQIS
                     ATVVDQKPVMVVIDSIQTTFSPYLESPPGTVGQIRECAAILISQIKKQGGMLFLVGHV
                     TKDGAIAGPKVLEHMVDTVLYFEGEKHHLFRILRAVKNRFGSTNEIGIFQMQEQGLVE
                     VANPSGLFLAERDARTSGSAVVASIEGTRPLL"
     gene            complement(983..>1318)
                     /locus_tag="AC16GM_04230"
     CDS             complement(983..>1318)
                     /locus_tag="AC16GM_04230"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2922918.1"
                     /translation="LSGGMRKRVALARASALDPEIILYYEPTTGLDPIMSDVINELVI
                     ALRNQLRITALAVTHDIVSAYKIADRVAMLYDGVIIHDGTPEETRQSTNPVVQQFIHG
                     QAEGPIQAV"
BASE COUNT          256 a          396 c          384 g          282 t
ORIGIN      
        1 ctaatagcgg ccgggtgccc tcaatgctcg ccaccacggc ggagccggag gtacgggcat
       61 cccgctcggc caaaaatagg ccggacgggt tggccacttc gactaagccc tgctcctgca
      121 tctggaaaat cccgatttca ttggtggagc cgaaacggtt cttcaccgcg cgcagaatgc
      181 ggaagaggtg gtgcttttcc ccctcgaagt agagcaccgt atcgaccata tgctccaaca
      241 ccttgggccc ggcaatggcg ccgtccttgg tgacgtggcc cactaaaaag agcatgccgc
      301 cctgcttttt tatctgggaa atcaatatcg ccgcacactc acggatttgc cccaccgtgc
      361 cgggaggcga ttcgaggtag ggggaaaatg tagtttgaat cgagtcgatc accaccatca
      421 ccggcttttg gtcaacgacg gtggccgata tctgctgggc atcggtttcg gtgagaatgg
      481 cgagtgctcc gagtccctcc agacccaagc gctcggcgcg catcttgatc tgacggtgcg
      541 actcttcgcc ggaaatgtaa aggacgccgc cgtagcgctt ggccaccgcc gctcccactt
      601 gcaaaagcag cgtggacttg ccgatgcccg gatcgccgcc aactaagatg gttgaaccat
      661 cgaccgcgcc gccgcctaaa atgcggtcga attcatccag tccggagagg tagcgctcgc
      721 gctctaaggg ttcgatggtg ctcagtgcga ccggtttctc ggacgcgact ttttctcccc
      781 ggccgcgctt tgtgggtgca agcgaaatct tttcttccac cagactgttc caggtgccgc
      841 attcggggca ttttcccagc cagcggctct gctgggcgcc gcaggactgg caggcgtaga
      901 tggttttgag cttcgctggt tgcatcaatt cttctttaaa gaagggtcgc gtgggtcaga
      961 gacccacgcc taccccaaga aattaaactg cctgaatcgg tccctccgcc tgaccgtgaa
     1021 tgaactgttg caccactggg ttcgttgact gccgcgtctc ttccggcgtc ccgtcgtgga
     1081 tgatgactcc gtcgtagagc atcgccaccc ggtcggcgat tttgtaagcc gaaacgatgt
     1141 cgtgggtcac tgccagagcg gtgatccgca gttgattgcg cagcgcgatg acgagctcgt
     1201 tgatcacatc ggacataatc ggatccaggc cggttgtggg ttcatagtag agaataattt
     1261 ccggatcgag cgcactggcc cgcgccagag ccacccgctt gcgcatcccc ccggagag
//
LOCUS       DBNGZD010000158         7001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_26068, whole genome shotgun sequence.
ACCESSION   DBNGZD010000158 DBNGZD010000000
VERSION     DBNGZD010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_26068"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..2109)
                     /locus_tag="AC16GM_04235"
     CDS             complement(373..2109)
                     /locus_tag="AC16GM_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2922919.1"
                     /translation="MLTRFKIRHFKRFEEVDIELGNPVVFIGPNNSGKTTALQALALW
                     QIGVHRWQEKRKGKTAPEKRPGVTISRRDLVAVPVPDANLLWLNLHVRDVQREDGKLK
                     TENIRVDILVEGVTAGKKWECGLEFDYANEESFYCRPLRLTGDASSERMLVPPEASSV
                     KIAFLPPMSGLAAQETRLDTGAVNVRIGEGRTAEVLRNLCYQLVSSGGESKSHKAINK
                     WEKLSRHINHFFGVTLNPPQYISERGEIVMSYREVGSGVTLDLSAAGRGLHQILLLLA
                     YLYANPGAVLLLDEPDAHLEILRQREIYQLLVETAREQSSQIIAASHSEVILNEAADR
                     DVVVAFVGKPHRIDDRGSQVLKALKSIGFEHYYLAETKGWVLYLEGATDLAILREFAQ
                     IIRHPVASPHLERPFVHYVENQPKKALDHFYGLREAKPDLLGIAIFDRRDPPPDPDPN
                     LKILMWQRCEVENYLFFPEVLLSYARGEGTPNLFTPVYEDTMRRCINDLVPPLALRDR
                     SDRWWSEVKASEFLDRLFKEYFQRLNLPNIMSKSNYHELARLVPENLIIPEVREKLDA
                     ILTIAEAARPHT"
     gene            complement(2215..3615)
                     /gene="mnmE"
                     /locus_tag="AC16GM_04240"
     CDS             complement(2215..3615)
                     /gene="mnmE"
                     /locus_tag="AC16GM_04240"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR2922920.1"
                     /translation="MSDTTIYPQDDTIAAIATPLGEGGLGIVRLSGKMAIEVAERLFQ
                     GKHNLLAAPSHTAHFGRIVDPKTGAIADEIVATVFREPHSYTGENLVELSCHGGPLVT
                     SKVLSIALENDSRLANPGEFTLRAFLNGRIDLTQAEAVVDIIRAKTDLSLQAGIEQLS
                     GKLSHVVNALREQLLDLLAHLEAAIDFPEEEIETKTASQLLGQLGEIQTKIKTLLATY
                     EEGRILRDGLSVVIAGRPNVGKSSLLNALLKEERAIVTPYAGTTRDVIKEYANFHGVP
                     VKLVDTAGLRPKGGKIEREGIRRTEKEIEKSDLILFVCDAQAGFGPRDRHLWQKLPEG
                     NRLAVINKIDLVSPKEIARAKTQLNDNNPISEVSALYGTGVEELKKTIAALALPTKKD
                     TAGEVVVTNFRHKEALEKARTGLARVEEGLQKKRGFELVAFDLRQATDHLGEIVGKIY
                     TENILGRIFSTFCIGK"
     gene            complement(3741..5417)
                     /gene="yidC"
                     /locus_tag="AC16GM_04245"
     CDS             complement(3741..5417)
                     /gene="yidC"
                     /locus_tag="AC16GM_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR2922921.1"
                     /translation="MNRQSITGIVLIVLLTIGYFIYVGKEAAKRKPLQRPTGTTDTTK
                     IPTPTPAAPDTGKEIAGVLPTLTLPEATPTGRMAAAVETVTVTTPLYIAQFENRGARL
                     VNFELTQFHEADGTQVNLVPQENRPTLSLLFPYENFDLNQHFLVPSAKEIKIDEKSGA
                     QVLTWILKQKKDSLLTLTYRFSPQTYAIDLQIKTYPSLAAGKEYRLGWLSGLSPTEKN
                     RAEDLSHFGASARMGEDYVKVTKFSDHRLHEAATGETWWAATRSKYFGVALIPRSRPG
                     AGFYTDGELTELELNGKKIPERRIAVELVMDVSPGKLVNDSFSVYLGPLDYKELRSFH
                     LGLENMLDLGFKIIKPFSIAILWCFQAVHKIIPSYGIVIILFTLVIKVLTFPLSRKMI
                     KSMQKMSALQPKMAALKERYKNDAKRMNAEMMKLYKEAGVNPLGGCLPMVLQLPLWWA
                     LFAVFRTTIQLRGTRFLWLPDLSQPDAFPSELFPLLPIVMALTMFWQQKLTTKDPKQK
                     MLVYLMPIMFFFFFRSFPTGLVLYWTMFNILSVAEIYLTGRHKTPAPMTA"
     gene            complement(5544..5753)
                     /gene="yidD"
                     /locus_tag="AC16GM_04250"
     CDS             complement(5544..5753)
                     /gene="yidD"
                     /locus_tag="AC16GM_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR2922922.1"
                     /translation="MKPLGIFLIRIYQHTLRYFFPASCRFYPSCSEYSAEAIKRYGFF
                     RGVWLGAGRLLRCHPFHAGGYDPLP"
     gene            complement(5750..6118)
                     /gene="rnpA"
                     /locus_tag="AC16GM_04255"
     CDS             complement(5750..6118)
                     /gene="rnpA"
                     /locus_tag="AC16GM_04255"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR2922923.1"
                     /translation="MAGEKPFGLPASVRLKKRKDVVRVLKQGKKVRQGPLECYFLRGE
                     SDGFRFGVQARSKLGIAVLRNRAKRLVREVVRLNKDQVPAGCDFFILVSKWEKAWGFE
                     EMKAHLLPLFDQVRTMATAQ"
     gene            complement(6127..6276)
                     /gene="rpmH"
                     /locus_tag="AC16GM_04260"
     CDS             complement(6127..6276)
                     /gene="rpmH"
                     /locus_tag="AC16GM_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR2922924.1"
                     /translation="MKRTYQPSRRKRLNDHGFRARMSTPGGRKVLSRRRAKGRWRLIV
                     EIGKK"
     gene            complement(6466..>7001)
                     /locus_tag="AC16GM_04265"
     CDS             complement(6466..>7001)
                     /locus_tag="AC16GM_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922925.1"
                     /translation="AFSPSENPVLATVAAWTPQSSCHATNISIWWLEWDFTLQFKFYD
                     TSIKEIAEIIKEVQEEVTPNHIARIRRLYQCADKLDLRYSKIWKREYLDKEYGNDNFR
                     LVEELYGEGRGMAVDMIDLSNLAERLEDFIGKKTKQKSNSEPAIDMLELKPNFFGLGI
                     NFNKVLKFFRRKRGVGT"
BASE COUNT         1616 a         1803 c         1822 g         1760 t
ORIGIN      
        1 gggtgatgat gtcggcggtc tggatcaaat ttatatcgcc gtcgagacga tagacccccg
       61 cgtcgtcgaa ggtgtcgatc cccacgacta catttttatt cttcgggatt ttgaggcccc
      121 gaacgatgtc ggataagtcc accggactta gcttaccggc tcaaccgccc gaacgtgcaa
      181 acgaggtaag tttgcaggtt tgaccttcca tcattccttt ctgtctcccg caggggacga
      241 ggttctacac catccaaaat aatacggaaa gatacccgat acaaattatc ggtaatgagc
      301 caaactgaga atgatgaaga aaaacgccgt gaccatttgg gaggtaatga ggagctttgt
      361 aatactgctt tcctatgtgt ggggtctcgc agcctctgca atcgttagaa tggcgtccag
      421 tttttcacgg acttccggga taatgagatt ttctggtact aaccgtgcca gttcatgata
      481 gttgctctta ctcattatgt tcggcaaatt taatcgttgg aaatattctt taaaaagacg
      541 gtcaagaaac tcgcttgcct ttacttcgct ccaccaccga tcacttctgt cacgcaatgc
      601 caatggcggt accaagtcgt taatgcatct ccgcattgtg tcttcataga caggtgtgaa
      661 taaattgggt gtcccctctc cccgagcgta ggacaacaga acttcaggaa aaaaaagata
      721 attttccact tcacatcgtt gccacattag tatttttaag tttggatcag gatctggtgg
      781 aggatcccgt ctgtcgaaga tcgcaattcc taacaagtct ggtttggctt ccctgagtcc
      841 gtagaagtga tctagcgctt tcttaggttg attctccaca taatggacga atgggcgttc
      901 aagatggggg gaagcaacag ggtgcctgat aatttgagcg aactctctaa gaattgctaa
      961 gtcagtcgca ccttcaaggt aaagaaccca accttttgtc tcagctaagt agtaatgttc
     1021 aaaaccaata gactttaggg ctttgagcac ctggctgccg cgatcatcaa tgcgatgagg
     1081 tttaccgaca aaggcaacaa caacatcacg gtctgccgct tcattgagaa tgacttctga
     1141 atgactggcg gcaataatct gactgctctg ctcacgagca gtttctacca aaagttgata
     1201 gatttcacgt tgacggagaa tttcaagatg ggcgtcgggt tcgtccagaa gtaacaccgc
     1261 cccggggttg gcatagagat aggccaataa aaggagaatc tgatggagac ctctccccgc
     1321 tgcagataga tccagagtga ctccactgcc cacctcgcgg taggacatta caatttcacc
     1381 ccgttctgat atgtattggg gcgggtttaa tgtgacacca aaaaaatggt tgatgtgcct
     1441 ggatagtttt tcccatttgt tgattgcttt atgggattta gattcacccc cagatgaaac
     1501 gagctggtag cacaggtttc tcaatacttc ggcagtgcga ccctcgccga ttcggacatt
     1561 aacagctcct gtgtccaatc tggtttcttg tgctgctagt cctgacatag gtggaaggaa
     1621 ggcaatctta accgaagaag cctccggtgg aacgagcatt ctttctgaac tggcgtcacc
     1681 cgttaaacgc agtgggcgac agtagaacga ttcttcattg gcataatcaa attccaaacc
     1741 gcattcccat tttttgcctg ccgttactcc ttccacgagg atatctacgc ggatgttttc
     1801 agttttaagt ttaccatctt ctcgctgaac atcgcggacg tgtaaattga gccagagaag
     1861 atttgcgtca ggtactggca cggcaacaag atcccggcgg ctaatagtta caccaggtct
     1921 cttctcagga gcagttttcc ctttgcgttt ttcctgccag cggtgtacac ctatttgcca
     1981 tagagcaagt gcctgcagcg cagtcgtctt tccggaattg ttgggaccta tgaacacaac
     2041 gggattgcct aactctatgt ccacctcctc aaaacgctta aagtgtctaa ttttgaagcg
     2101 cgttagcact gttaagaacc tcctttaatt ttcaagtcac tttcatttcc tataaatgaa
     2161 gttttaataa tacttcaaag actgtccaac tcaaaatcgt gctcagtctg aattttactt
     2221 tcctatacaa aacgtcgaaa aaatccgtcc cagaatattc tccgtataaa tcttccccac
     2281 gatttctcca agatggtcgg tggcctggcg caggtcgaag gcaaccaact caaagccgcg
     2341 ttttttctgc agcccctcct ccacccgggc caagccggtc cgggctttct ccaacgcttc
     2401 cttatggcgg aaattggtga ccaccacttc acccgcggta tctttcttgg tggggagcgc
     2461 gagtgcggca atggtctttt tcagttcctc cacgccggtg ccgtagaggg ccgagacctc
     2521 acttatcggg ttattgtcgt ttaattgtgt tttcgcgcgg gcaatctctt tcggggatac
     2581 gaggtcaatt ttgttgatga ccgcgagacg attgccctcg ggtagtttct gccacaggtg
     2641 ccggtcgcgg gggccaaagc cggcctgcgc atcgcagacg aaaaggatga ggtcgctttt
     2701 ttcgatttcc ttttccgttc ggcgaatccc ctcccgctcg atttttccgc ctttgggcct
     2761 gagaccggcg gtatcgacca acttcaccgg tacgccgtgg aagttggcgt attctttgat
     2821 cacatcccgc gtcgtcccgg cgtagggcgt cacgattgcg cgttcttctt tcaggagcgc
     2881 attcaacaga ctcgacttcc ccacgttcgg cctgcccgcg atcacgaccg agagcccgtc
     2941 gcgcaggatg cggccttcct cgtaggttgc gagcagtgtt ttgatctttg tttgaatctc
     3001 acccaattgg ccgagcaatt gactcgcggt tttagtttcg atctcctcct ccgggaaatc
     3061 aatcgccgct tccaggtgcg ctaagaggtc gagcagttgc tcgcgcaggg cgttgaccac
     3121 gtgggacagt ttgcccgaca gttgctcgat tcccgcctga agcgaaaggt cggtcttggc
     3181 gcggatgatg tcgaccaccg cttcggcctg ggttaagtcg atccggccgt tcagaaacgc
     3241 ccgcagggtg aattcacccg gattggctaa gcggctgtcg ttttccaacg caatcgaaag
     3301 cactttactc gttaccagcg gcccgccgtg gcaggagagt tccaccagat tctctccggt
     3361 gtaggagtgc ggctcgcgga aaaccgtcgc tacgatctcg tcagcaatcg ctccggtctt
     3421 cgggtcgacg attcggccaa agtgggcagt gtgggaaggt gcggccaaga gattgtgctt
     3481 tccctgaaag agccgctcgg cgacttcaat ggccatcttc cccgaaagac gcacaatccc
     3541 cagcccgcct tcccccaggg gcgtggcaat ggcggcaatg gtgtcgtcct gcgggtatat
     3601 tgtcgtgtct gacatcggaa ataaatatag caagaaatgt aggggcgtac cttcaggtgc
     3661 gcccattcct gggcgaatct ccttcgacta cgcttaggac aagtgaagat tcgcccctac
     3721 atttattcca aggggaaatt ttacgccgtc atcggcgccg gggtcttgtg ccgcccggtc
     3781 aggtaaatct ccgccaccga gaggatgttg aacatcgtcc agtagagtac gagaccggtg
     3841 ggaaaggagc ggaagaaaaa gaagaacata atcggcatca ggtaaaccag catcttctgc
     3901 ttggggtctt tggtggtcaa tttctgctgc cagaacatgg tcagggccat gacgatcggc
     3961 agaagcggga aaagttcgga aggaaaagcg tccggctggg agaggtcggg cagccagagg
     4021 aaccgcgtcc cgcgcagctg gatcgtggtc cggaaaaccg caaagagcgc ccaccagagg
     4081 gggagctgca acaccatcgg cagacaaccg cccagaggat tgacgccggc ttccttgtag
     4141 agtttcatca tttcggcgtt catccgtttg gcatcgttct tgtagcgctc ctttaaggcc
     4201 gccatcttcg gctgcaaggc cgacatcttc tgcatcgact tgatcatctt ccgcgagagg
     4261 ggaaaggtga gcaccttgat tacgagcgta aagaggatga tgacgatacc gtaactgggg
     4321 atgattttat gcaccgcctg aaagcaccag aggatggcaa tcgagaaggg cttgatgatc
     4381 ttgaagccca gatcgagcat gttttccagg ccgaggtgga acgagcgcag ctctttgtag
     4441 tcgagggggc ctaaataaac tgaaaatgag tcgttcacta acttgcccgg agaaacatcc
     4501 atcaccagtt cgacggcaat gcgccgctcc gggatttttt tgccgttcaa ttcgagttcg
     4561 gtcagttcac cgtcggtata aaagccggcg ccgggacggg agcgggggat cagcgctaca
     4621 ccgaagtatt tcgaccgggt ggcggcccac caagtctccc ccgtcgccgc ttcgtgcagg
     4681 cggtggtcgg agaatttggt gaccttgacg taatcttctc ccatccgcgc ggaagcgccg
     4741 aagtgggaga gatcctcggc gcggttcttt tccgtggggg aaaggccgga aagccagccc
     4801 aggcgatact ctttgcccgc ggcgagagac gggtaggtct ttatctgtaa atcgatggca
     4861 tacgtctggg gagaaaaccg atacgtcaag gtgagcagag aatctttctt ctgcttcaat
     4921 atccaggtga ggacttgcgc accgcttttt tcgtcgattt taatttcttt tgcgctagga
     4981 acgaggaagt gctggttgag gtcaaaattc tcgtaaggga aaagaagcga gagcgtgggc
     5041 cggttttcct gcggcacgag gttcacctgc gtgccgtcgg cttcgtggaa ctgcgtcaat
     5101 tcgaaattga ccagccgggc accgcggttt tcgaactgcg cgatgtagag gggggtagtt
     5161 accgtcaccg tctccactgc cgcagccata cggcccgtag gggtggcttc gggcaaagtc
     5221 aatgtgggca aaactccggc gatctctttg cccgtgtcgg gcgctgcggg agttggcgtt
     5281 ggaatcttcg ttgtatcggt agtgcccgtc ggacgctgga gcggtttgcg cttggctgct
     5341 tcttttccga cgtaaatgaa atagccaatc gtcagaagga cgatgaggac gattccggtg
     5401 atgctctgtc ggttcaaatg aaatgcactc ccggtttatc aatgttgggg tgaatgtagg
     5461 ggcgaacctc gtgttcgccc tgattggttt gcccaacacc cgtatgcggg cgaataccag
     5521 attcgcccct acaaggaaaa acgttatggt agcggatcat atccccccgc atggaacggg
     5581 tggcagcgca gcaaccgccc cgcacccagc cagacgcccc tgaagaagcc gtaccgcttg
     5641 atcgcctcag ccgaatactc cgagcaggac ggatagaacc ggcaggaggc gggaaagaaa
     5701 taccggagcg tgtgctgata gattctaatg agaaaaatgc cgagcggctt cattgagcag
     5761 ttgccatagt acgtacctgg tcgaacaaag gcaagaggtg ggctttcatt tcttcaaagc
     5821 cccaggcctt ttcccacttg gacactaaaa tgaaaaaatc acacccggcg ggcacttggt
     5881 ctttgttcaa ccgcaccact tcgcggacta accgcttggc ccggttgcgc aggacggcga
     5941 tgcccagttt ggagcgggcc tgcacgccaa agcggaaccc gtcactttct ccacgcaaaa
     6001 aatagcactc cagcgggccc tggcggactt ttttcccctg cttcagcacc cggacgacgt
     6061 ctttgcgctt tttgagccgg actgaggccg gtaagccgaa cggcttctct cctgccattg
     6121 gcaggattac tttttgccaa tttccacgat caggcgccag cggcccttgg cccggcggcg
     6181 ggagagcacc ttgcggccgc ccggagtgga catgcgggca cgaaaaccgt ggtcattcag
     6241 ccgcttgcgc cgtgagggtt ggtatgtgcg tttcatttaa ttcctttcca gtacgatctc
     6301 ttaccattct tcatctatta tttttgagta aacaattcaa tataattcaa taagaaccac
     6361 cgtcaaggga ctgaaggtca caccattgcg agattaggaa acttcctcat tctattttct
     6421 tgaccgccca attcgcttta aaagaacatt taaccgcaat ccatgttatg tacccacgcc
     6481 ccgttttctt cggaaaaact tgagcacttt gttaaagttg attccgagac caaaaaaatt
     6541 tggcttcagt tctaacatgt caatggcggg ctcagaattc gatttttgtt tggttttctt
     6601 accaataaaa tcctctaaac gttctgcgag attggacaga tcgatcatat caacagccat
     6661 accacgtcct tctccgtata gttcctccac taaccggaaa ttatcattgc catattcttt
     6721 gtcgagatat tctcgtttcc agattttact atatctaaga tcgagtttgt cagcacactg
     6781 atataaacga cgaattctgg cgatatggtt tggtgtgact tcttcttgta cttcttttat
     6841 tatttcagca atctccttaa tgctggtatc ataaaactta aattgaaggg taaagtccca
     6901 ttccagccac caaattgata tgttggtggc atggcaagac gactgaggtg tccacgctgc
     6961 gacagtggca agtactggat tctccgaggg agaaaacgcc g
//
LOCUS       DBNGZD010000159         5267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_929089, whole genome shotgun sequence.
ACCESSION   DBNGZD010000159 DBNGZD010000000
VERSION     DBNGZD010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_929089"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1795
                     /locus_tag="AC16GM_04270"
     CDS             212..1795
                     /locus_tag="AC16GM_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2922926.1"
                     /translation="MDGVVALTNFKTPAPAPESNSPYTTSGMKSKALCRLRWFISVTS
                     AGSFVCLSCVGPPPEVAPVENPGLDPQVRQYWEIKAAASTDEFTAYEATPDSLKPDWV
                     RRFWKSKDPTPATPENEFLTEHGRRVDYALQHYGHQGKGRPWDDRGEMYIRYGEPDEI
                     EMSDEGFYDPGAPKSAIELRDNPIAAKQAATRRGTGAETSIGGDPLFATSIERWYYSR
                     YGLSFQFQPDLATGLVEAVPYVSTDRGQVQTAREFVNSTEIRHEIYQHDYGGDFLPYT
                     FSIARFRGPARNWEVDVNLALPLEKLALAGPDTNEVSCLRRMVIFDQAWHEVALDSTV
                     IVKEVERYRRRGKLLVEQKTFQVPPGLYTLSVEVRDLITRKVGIYKKPLLLPEYAAPD
                     VQEVSDVVLASYVKPAEPSSLAFVRNGWVVMPQPSRVFFPDDPLAFYFEVYNLRRGPD
                     GKTHVKVGYEVVDFKSKKKIVTPLPQALVSDSTDLVRTGTVDIKTLPPGDYILAVRVV
                     DETTRKEKSALASFRIVKG"
     gene            2029..3810
                     /locus_tag="AC16GM_04275"
     CDS             2029..3810
                     /locus_tag="AC16GM_04275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR2922927.1"
                     /translation="MTIDTEKTEGATVTYRGPAHAHACLFRDVAKIVSSELEIDALVQ
                     VTLELLVKTFNAGAGALHLFHPETGKVHLYVSDQDGNRAPREISYRPGEGFLGWVAEK
                     REPLLVNDTTHDLRSAPVLESEFVSPVRSLVVVPLVWRDNLYGVLELINKVEGDFTSE
                     DRDTLGALADQIGIAFRNASLYEKLKKESLQRERLFEIGKYLSSSLELDEVLEKILDA
                     LHQVVPYSVAGIFLVDRKSQTVNTFHTRGYDPALAADLRLKIGQGLVGYVAKTGEPVI
                     VPDVAVDPRYINAHSETQSEMVAPIVAGGKVIGVFNLEANRKAAFSPADLELLNAFAG
                     QAALTIERARLYREILGKKRLEDELKIARQIQVSFLPTKNPTVRGFDIAGLNISSQQV
                     GGDYYDFIPIVEGQMGIAIADSIGKGIPAALVMASFRASLLAEIRNNYAIRTILGKVN
                     RLLWESLEQGNFVTAVYGVLDTQNRILTYSNAGHNQPILRRASGKIDYLAEGGMALAI
                     SPDAAYAERRFTLRAGDILVFYTDGVTEAKNERNEEFGQGRLEALLHQPPNLSAEALA
                     HKIVDEVKMFAAPTHQMDDLTLVVVRA"
     gene            3962..>5267
                     /locus_tag="AC16GM_04280"
     CDS             3962..>5267
                     /locus_tag="AC16GM_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR2922928.1"
                     /translation="MTKKFKASSIFLLLFGTLLWGGVTLYLNLTLKKSFSITYDGATW
                     KTASGGLVIDSLVPGDPVDRAGLLRTDTLLLANGVSVTSDPALQDVLDRTPAGRPATY
                     TVRRGADLVKLEVPVYRYRSEIIWHWLLQLAGFVYIVLAVLVYAKKPESKTARTFFFF
                     GLVAGSLVATIWVNEPSPNKYFDLFTNVWLYYNIFFINALGLDFIRRLMFFSPGQEPK
                     KRLLLLNYLPALLGLALYLAPLVLSLAHGQFVIKAPVGFWNKIQKIIFPATYGLYLVW
                     FFAEIATRARKKQFAINRTQGEWLRWGVGLPMALFVVLEILLPAFFKIELIKWSRVIL
                     LPPPFVFAYLILKERILDVGVVIKRSAIYAILSALLIGTFVVLVMAVSQLAVLVTGQE
                     SKLAVFASALLTAGVFNLYRQKTQNYLDRKFFKDRHNYQKALL"
BASE COUNT         1110 a         1536 c         1477 g         1144 t
ORIGIN      
        1 atttaccaga ccggcaagat caacctgcgc gatctggccc ccggccagta cattctctcg
       61 ctcaaggtga aagattacac gaccggcaag gaaaagaccg ccctggcggg gttcaagatc
      121 gccaggagtg agtgatctca gaatttttgt tcctgccata gccgaggttt tcagcctcgg
      181 attccattca ttcaggctgg aagcctgacc tatggacggg gttgtagccc tgaccaattt
      241 caaaactccc gctcctgcgc cagagtcgaa ttctccgtat actaccagtg gaatgaaatc
      301 aaaagcactc tgccgtcttc ggtggttcat ctcggtcacc agcgccggaa gctttgtttg
      361 tctctcctgt gtggggccgc cgccggaagt tgccccggta gagaatcctg gtctggatcc
      421 ccaggtgcgc cagtactggg agatcaaagc ggcggcttcc acggacgaat ttaccgccta
      481 cgaggcgacg ccggacagtc tcaagcccga ctgggtgcgg cgtttctgga aatcgaaaga
      541 ccccactccc gccacgccgg agaatgaatt tttgaccgag cacggccgcc gggtcgacta
      601 cgccctgcag cactacggcc accagggaaa gggccgcccc tgggacgacc ggggagagat
      661 gtacattcgc tacggagagc cggacgaaat cgaaatgagc gacgaaggat tttacgaccc
      721 cggcgcgccg aagtcggcca ttgaactgcg cgacaatccc atcgccgcca agcaggcggc
      781 cacgcggcgc ggcaccggcg cagaaacctc aattggcggg gacccacttt tcgccacctc
      841 catcgagcgc tggtactact cccgttacgg gttgtcgttt cagttccagc ccgacctcgc
      901 caccgggctg gtcgaggcag tgccgtacgt ttccaccgac cggggccagg tgcagaccgc
      961 gcgcgagttc gtcaactcca ccgagatccg gcacgaaatc taccagcacg actacggcgg
     1021 cgacttcctc ccgtacacct tcagcatcgc ccgcttccgc ggtccggcac gaaactggga
     1081 agtcgacgtc aacttagcgc tccctctgga gaaactcgct ttagccgggc cggataccaa
     1141 cgaggtgtcc tgtctcaggc ggatggtgat tttcgatcag gcctggcacg aagtggcttt
     1201 ggacagtacg gtaatcgtca aggaagtgga gcgctaccgg cggagaggga agcttttggt
     1261 ggagcagaaa acttttcagg tgccgcccgg gctctacact ctttcggtgg aggtgcgcga
     1321 cctcatcacc cggaaggtgg gcatctacaa aaaacctctg cttttgccgg aatacgccgc
     1381 acccgacgtg caggaagtat ccgacgtggt cctagcctcc tacgtcaaac ccgccgagcc
     1441 ttccagtctg gcgttcgtgc gcaacgggtg ggtggtgatg ccgcagccgt cgcgggtttt
     1501 ctttccggac gatcccttgg cgttctactt cgaagtgtac aatctccgtc gggggccgga
     1561 cggcaaaacc cacgtcaaag tgggctacga agtggtggat ttcaaaagca aaaagaaaat
     1621 cgtgacgcct ttgccccagg ctctggtttc cgacagcacc gatttagtcc gcaccggcac
     1681 agtcgacatc aagacgctgc cgccgggcga ctacatcttg gccgtccgcg tggtggatga
     1741 aaccacccgc aaggagaagt cagccctggc cagtttccgg attgtgaaag gctgaaagaa
     1801 ttaagcattg cgcggtgggg attgaggggg aaagcgctcg tgcattcgat gtgagctcta
     1861 aaacgtttct tcaactcccg acttcccgct aaatttcctc cgagaatctg tcgagagaaa
     1921 tacgagaggg tgctccagtc gtgttccgga catttcgaat tgacagttct actttgcaaa
     1981 aataagttac gaactccggc aaaaggaaaa cgtatacctt aagtagtgat gactatcgac
     2041 acagaaaaaa cagaaggtgc gaccgtcacc taccggggac cagcgcacgc ccacgcctgt
     2101 ttattccggg acgtggccaa aatcgtcagt tcggagttgg aaattgacgc gctggtgcag
     2161 gtgacgctcg aacttttggt caagaccttc aacgccggtg ccggtgcact ccaccttttc
     2221 cacccggaga ccggtaaagt ccacctctac gtctccgacc aggacggtaa ccgtgcgcca
     2281 agggaaatta gctaccggcc cggggaggga ttcctcggct gggtggccga gaagcgcgaa
     2341 ccgctcctgg tcaacgatac cacccacgac ctacgttccg ccccggttct tgagtctgaa
     2401 ttcgtctctc cggtgcgctc gctggtggtc gtacccttag tctggcgcga caatctgtac
     2461 ggtgtcctgg aactcattaa taaggtcgaa ggagacttta cctcggagga tcgggatacc
     2521 ctgggggcct tggcggatca gatcggcatt gcctttcgca acgcctccct ctacgaaaag
     2581 ttgaagaagg aaagcctgca gcgcgagcgg ctgtttgaaa tcggcaagta cctgtcgtcg
     2641 tctctggagt tagatgaagt cttagagaaa atcttagacg cgctgcacca ggtcgtacca
     2701 tacagcgtgg cgggtatctt cctggttgac cggaagagtc aaacggtcaa cactttccac
     2761 acccgcggct acgatccggc tttagccgcc gatctccggc tcaagatcgg ccaggggctg
     2821 gtcggctacg tggccaagac cggcgaaccg gtgatcgtgc ccgacgtggc agtggacccg
     2881 cgctacatca atgcccactc cgaaacgcag tcagagatgg tggccccgat tgtggccggc
     2941 ggaaaggtga ttggagtctt caacctcgaa gcgaaccgga aagccgcatt cagtccggca
     3001 gatctggagt tgctcaacgc ttttgcgggt caggcggccc tgaccatcga gcgggcgcga
     3061 ctctaccggg aaattttggg caagaagcgc ctggaagacg aactgaaaat cgcccggcag
     3121 atccaggtct cgtttctgcc gacgaaaaac cctaccgtca ggggttttga catcgccgga
     3181 ctgaacatct cctcccaaca ggtgggcggc gactactacg acttcattcc catcgtcgaa
     3241 gggcagatgg gcattgccat cgccgattcg atcggcaagg ggattcccgc ggcgttggtg
     3301 atggcctcct tccgcgcgtc gcttttagct gaaatccgca acaactacgc catccgcacc
     3361 atcctgggga aggtgaaccg gctgttgtgg gaatcgctgg aacaagggaa tttcgtcacc
     3421 gcggtctacg gcgttctgga cacccagaac cgcatcctca cctactcgaa tgccgggcac
     3481 aaccagccga ttctccggcg ggcgagtggc aagatcgact acctggccga agggggaatg
     3541 gcgctggcga tttctccgga cgccgcctac gccgagcgcc gcttcaccct gcgcgcgggg
     3601 gatattctgg ttttttacac cgacggggtg accgaagcca aaaacgagag gaacgaggag
     3661 ttcggccagg gacggttaga agcactcctg caccagcctc ccaacctctc ggcggaggcc
     3721 ctggcccaca agatcgtcga cgaagtcaaa atgttcgccg ccccgacgca ccagatggac
     3781 gacctgacgc tcgtggtcgt ccgcgcctga agagctttcc ctgcgaaaag tttctctttc
     3841 cggctttggg tttgtatatt tttgccgtga tcgagagaag tcttgcgcga gggtggggaa
     3901 cgtacacggg ggtgtgtccc gcaagccaat tgatccctcc ggcggccggt aatttctcac
     3961 cgtgaccaaa aagtttaaag cctcgtctat ttttctcctc ctctttggaa ccttgctctg
     4021 gggtggggtt accctctatt tgaacctgac cctgaagaag tctttcagta taacgtacga
     4081 tggtgccacc tggaaaaccg cttccggggg cctggtcatc gattcccttg tcccgggtga
     4141 cccggtggat cgagccggcc tcctccgtac ggacactttg ctattggcga acggtgtcag
     4201 cgtgacctcc gatccggctc tgcaggacgt cttagaccgg actcccgccg gccggccggc
     4261 cacctacacc gtccggcgcg gagctgattt ggtaaagttg gaagttccgg tgtaccggta
     4321 ccgatcagag atcatctggc actggctttt gcaattggcg gggttcgtct acatcgttct
     4381 ggcggttctc gtctatgcaa agaagccgga gtcaaaaaca gcacgtacct tcttcttttt
     4441 cggtctggtc gccggctccc tggtggcgac catttgggtg aacgagccca gtccgaacaa
     4501 atacttcgat ctcttcacca acgtttggct ctactacaac atttttttca tcaatgccct
     4561 cgggctcgat ttcattagac ggcttatgtt tttttcgccc ggtcaagagc ccaagaaaag
     4621 gctattgctc ttaaattatc ttcccgctct gctcggactg gccttgtatt tggcacccct
     4681 ggttctctcc ctggcccacg gccaattcgt cattaaagca cccgttggtt tttggaacaa
     4741 aattcagaaa ataatctttc cggccaccta cggtctctat ttagtctggt tttttgccga
     4801 aattgccact cgcgccagga aaaaacagtt cgccatcaat cgcactcagg gggaatggct
     4861 gcggtgggga gtgggcctgc cgatggcact tttcgttgtt ttggaaattc tcctgccggc
     4921 ttttttcaag attgagctta taaagtggag ccgggtcatc cttttgcccc ccccttttgt
     4981 cttcgcctac ttgatattga aagagcggat tttggatgtc ggcgtggtga tcaagcgctc
     5041 ggccatctac gccatcctga gcgcgctttt aatcggcacc tttgtcgttc tcgtgatggc
     5101 cgtaagccag ctggccgtgc tcgtaacggg gcaagagtcg aaactggcgg tgtttgccag
     5161 cgcactccta actgccgggg tgttcaattt gtaccggcag aaaacccaaa attatctcga
     5221 ccggaaattc ttcaaagacc gccacaacta tcagaaagca ctcctcg
//
LOCUS       DBNGZD010000160        27861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_235031, whole genome shotgun sequence.
ACCESSION   DBNGZD010000160 DBNGZD010000000
VERSION     DBNGZD010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 27861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27861
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_235031"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC16GM_04285"
     CDS             complement(<1..474)
                     /locus_tag="AC16GM_04285"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR2922929.1"
                     /translation="MIGQTISHYKIVEKLGEGGMGVVYKALDTKLKRPVALKFLPLDL
                     TRDEETKLRFVHEAQAASALDHPNIAVVHEIDETHDGRSFICMAYYEGETLKKKIEAG
                     PLKIEEAIAIALQIAEGLQRAHEAGIVHRDIKPSNVIVTSQGHVKIVDFGLAKLAG"
     gene            complement(471..3407)
                     /locus_tag="AC16GM_04290"
     CDS             complement(471..3407)
                     /locus_tag="AC16GM_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2922930.1"
                     /translation="MLGKIVSHYKIVEKLGEGGMGVVYKALDLKLHRPVAVKFLPPHV
                     ADSPDEKARFLHEAQSASALNHPNITTIYGIEETPEGIFIAMEYVEGKTLKQVVEKET
                     LPLKKILEIASQVCEGLTAAHKKEVVHRDIKSENIMVTREGLVKLMDFGLALLKGTSR
                     LTRAGSTLGTAAYMSPEQASGEEIDQRSDIFSFGVVLYELLTGKLPFPGEHQAAIIFS
                     ILNEQPQPVARYNSKVSPELERIVAKTLAKDKEERYQHIDDLLADLRREKKQLEYVKA
                     SQIPPEAAAARPKKKLLPILVPASLVFAAAFLFFILNPFKIEIGSEKKATAAENSLAV
                     MYFDNVADPEDRDRIVQMITALLITDLSESQYLQVVSRQRLYDILKLVGKENLKSIDK
                     SVASEVAQRAGVKWVLTGSVLQTKPNIVLVSDIADAASGKVRASERITGEAGQDIFAV
                     VDRLSAEIKKDLALPTQAAQEPDKPVADATTHSPEAYRYYLEGVDYWEKLYDPEAVKS
                     FRKAIELDSTFTMAYFYLARVLTRLQNPDAKKAIAQAVKYSNKVSEMERHYIRALEAR
                     LAGNFPLAIKEWEKIAADYPQEKDAYISLGATYANALQQPEKAVGPLTKAIEIDPLVK
                     AAYNLLAYAYNDLGDFEKSIWAINKYISLAPEEANPYDTRGDLYAYNGKLDEAIESYR
                     KALEKKPDFYSSLTKLGYLYLFKREYVRAESSFQALAASSEKDIRSEGRLHLAQIPVY
                     QGRFNEALQVLDQGIAADQFERAEGTALPRKHAVKARIYEEKKNLKLALEEVKKCMEV
                     YHKAFPSDAANFRGFYAAFLAKSGEIDKAEEVARTFKKDIEKTDTTRMWLYWVATGWI
                     ELTKGNLPVAVAQLAKAGKAHTDFPVHYLLAEAYLEAGRLGEAVSEFEKILSRYDEIR
                     SFFLTWSVKANYFLGLAYEKSGWNQKAIEKYQEFLDIWKNADPGIPEVEDAQKRLAKL
                     KQSAAK"
     gene            complement(3491..3970)
                     /gene="coaD"
                     /locus_tag="AC16GM_04295"
     CDS             complement(3491..3970)
                     /gene="coaD"
                     /locus_tag="AC16GM_04295"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR2922931.1"
                     /translation="MKIAIYPGTFDPITNGHRDLIKRALELFDELVVAIAENPEKHPL
                     FTLEERLEMVKATVASLSGVKVMTFAGLTAELTTKAGAVAILRGLRAVSDFEFEFQIA
                     LMNRRLAPKAETVFLMPSEQFTYLSSNVIKDVARHSGDITAFVPPIVAQKLKAKFKS"
     gene            complement(4047..4616)
                     /gene="rsmD"
                     /locus_tag="AC16GM_04300"
     CDS             complement(4047..4616)
                     /gene="rsmD"
                     /locus_tag="AC16GM_04300"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR2922932.1"
                     /translation="MRIIAGFAKKRKLFTEPRQRRSLRYSSDKTKGAIFNILGATVVC
                     ARVLDLYAGSGNLGLEALSRGAAGVTFVEKRFDAVNLIKRNAALLGVAEKITVLRGDV
                     QTVVERLAHQGQAFDLVLADPPYREGLCRKLVILLSGNSVLKPEGFLVCEHDKREVLP
                     EQFETLALVKHRVFGDTALSIYQYRPGAA"
     gene            complement(4788..5741)
                     /gene="pilM"
                     /locus_tag="AC16GM_04305"
     CDS             complement(4788..5741)
                     /gene="pilM"
                     /locus_tag="AC16GM_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR2922933.1"
                     /translation="MTRFQQKTIGIATGGAAVTYAVVETSGSQRTITDLGAEQAKTKK
                     DPVTAVVKALVARKGNTAAWRNGELRLNLERPAWLNRKVTYPAELAGLYPHWPEWEMR
                     QGLLAAPEGLVIDFWKKALQTGERATAQAVVTRREPVEMTQRILDEAGLECQVFLATP
                     LALWAAHQTLSAGLAGTTVLLDVGEEVTNLAVGKDGELDYIEALTTPPGLFDTPPRQL
                     NEGFVLLKSELTRALAEEVATLLRSQGPDLQNGKIALSGPGASDAEIQKAIATALSLP
                     VEPANLLAACDWSSELKPKREDFLSQSHRYSVALGLALAPH"
     gene            complement(5746..6150)
                     /locus_tag="AC16GM_04310"
     CDS             complement(5746..6150)
                     /locus_tag="AC16GM_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR2922934.1"
                     /translation="MTLWFTRLQRIAVLVLAVTFLIGAGLWLQKREKTRLEPELIFRT
                     KSITPGPPASLAPETSGTLLPQFQLGQKVDLNTATGEQLAALPGIGPVLAQRIITTRQ
                     KYGPFQKAGDLLRVPGIGRKKLQQIAPLVETK"
     gene            complement(6214..7131)
                     /locus_tag="AC16GM_04315"
     CDS             complement(6214..7131)
                     /locus_tag="AC16GM_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR2922935.1"
                     /translation="MLHRPRSREILWQSLLFGCFLFWSGGCVSRSLPGFDGRRAYRTL
                     NDFYALGPKVPGTGTHERGLVFLLDTLQPFAAQVDTQQFSLSRGKSEIRLTNVIARFA
                     PEKRERLWLCAHWDTRPRAERDPDSTKRNEPTGGANDGASGVAVLLELARLLHEHPPE
                     VGIDLIFFDGEDFGPEGELDWYFLGSRYFARHLPPQRAELAILLDMVGDRDLAIYREQ
                     NSVRYAPEVVEKVWRKARELGVTTFRDSAKYSVEDDHLQLLWAGIPAIDVIDFDYPWW
                     HTTGDTPDKCSAQSLAAIGRVLAAFIYSR"
     gene            complement(7135..13029)
                     /gene="sov"
                     /locus_tag="AC16GM_04320"
                     /gene_synonym="sprA"
     CDS             complement(7135..13029)
                     /gene="sov"
                     /locus_tag="AC16GM_04320"
                     /gene_synonym="sprA"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS outer membrane translocon Sov/SprA"
                     /protein_id="HIR2922936.1"
                     /translation="MDSVPLLSLPASLKPHTAALDSSGGAIRLSNGSPDDPLLLDLSA
                     YRDWRSQTSYLDEWGKLKATSIRKPESQKAGGLTTFQIPWKPPKAVANIIGEGSAGLK
                     VSGYRRITMGGRSNWVDGLERTATYRASKFPTIQMKQDYQFNISGTVGSKITIDLRQN
                     SNQNVFDQANLADRIQLRYKGDEDEVIRSIEAGNTNLSLPGTQFVGYSEQKQGLFGIK
                     TQAKLGGLDLTMITSQDKSNSQRFNFHAGAQGATIYLRDYQYLRYAYYDLGRNTKADP
                     QRGVNEFDPARDSIIQLEVYRWKSSTAPGTGSIALPGICYVDPNDPNRPDVDTLERET
                     GYFERLKPDSNYFVNSLLRPFVVYFEPQLQESDIIGVYLKIKRSNPDTTIEYGCIDLA
                     ACDSILKLKLIKRRRGGSRPTDVTYSYEWKNVYNLGARNIDWDGLNVQIFKGSQGDES
                     NPARQEHDPGLANYLQVTGIDQFNALGVRLIDNKVDKREEVLDLRRGHLFFPERYPFN
                     SDVSYAGDTATSLRTRIAAIYDSINVPDVLQRNTQYYLEIKSLTGRQKDFSLGRVNIL
                     ENSEEVLLNNRRLVRGKDYNIDYQLGQISFLNEEVLNPNADLSINFDYSPFLSTEKKA
                     LFGLRGDYQLSDNFRAGTTVLFRNETSNDQKPKIGQEPNRTLLWDADASYRFQAPWLT
                     RAVNALPLVEAAAPSTVNLSGEIARSFPNPNTRQNAYLDDFEGSKETVDLGIFREGWT
                     AASPPVGKGPSSRGRLHWYNPDGQDWEQRFRVKDIWPDRDTRTVDERTDVLKFVFVPK
                     DTGAAPPESSWGGIMRSFFGGGVNLSQAQNLEVWATGDSGILHFDFGKIQEDLDSNGI
                     LNTEDSTVNGIRDNILTPGEDIGLDGQPDGNEPPAFGGTIGDPGGDNWHSRPDQINGT
                     EGNRFDPGRRDRPDTEDLNGDGALETHNEYFEFTLDLADTTSPFIVPGTLGRFWSQPG
                     NDFFTGTSYGTRWRLYRIPLRDSLIYRKEGRPDWNSIEYVRFWVTGVRDTARFAIATQ
                     AVVGNRWRELGLTPKDSISPDEKFRVAVINSQENKNYQIPPGVTETIDRITNVRQQEQ
                     SLVLQYENLGPGHTGLAQRTILGTAEDYSGYRRMHMFVHGPSGPGELLFFLRFGSGTN
                     ADTNNYYEYRTKVYPGWDSRNEVVIDFDRMTEIKGLLLKNHPSGGAQVPDTLTGNYRI
                     RGNPTLGSVRWFTMGVTNADTAGGPPASGEIWADELRVTDVRRDAGLAARFSAGAQFS
                     DFIDLKVDYLHRDAFFRNLTSGDRSGSLAASSYRFSSTQNNLTVSTQLALQKFLPPRW
                     GATLPLSINYRNGSSVPRLKNGSDIVLAPALRAEESTTNQDFNLTLSHQFRIETKNWL
                     VNLTLNRLGQFTYAYGRSQSHTPTLPKNDQSRYDVSGNYDLSPRTQLGIPWLAWSKWL
                     LVPRSIAKSKLQLLPSSFTLRGSLSRVLTEQVNSSQRPTTVFTRNFSGGWSTRFQPFS
                     PLSVTLDGTTQRDLANPDNLHFSLNPKTARFGFETSRTQAFGANYTPRIFSFMDNSLS
                     YSANYAENANPQQTVYGRTVTLTSGYGASSLVNLPNLYQATLGHFSFGKPAQQPKKPA
                     TVPQSVSPPAGANRDSLERAGRTLSADSTDSVKAGAPKGPGFSPLRLVSRSLYRGLQI
                     IDPVRLSYKKDRNLSRQALPGSPQQTFRFGFSEKTGFSTTSLGDQSSFADNYSATSGL
                     KLGGGFDLDVAYSRRVGTSQTQTSNTRSTSRVFPDLRFSMTNVDRFGLLKKLISSSQL
                     QSNYAFKSDAGLDLNRRHPTARTTTATFQPLAGLTLNWKNGMSTTLQSNQSTAKSQDL
                     SIGSANTRTENTSRGFTLSTSYSFRAPHGIQLPLLRGIRLQSTLRLDLAVTKRRDTQK
                     QAPGGRPLAILTDNSIFNLGLTGNYSFSSQVNGGLQVIWNDTNDLIRKRKSHTRELNF
                     YMELRF"
     gene            complement(13287..14282)
                     /gene="murB"
                     /locus_tag="AC16GM_04325"
     CDS             complement(13287..14282)
                     /gene="murB"
                     /locus_tag="AC16GM_04325"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR2922937.1"
                     /translation="MLKLQEGTQRDVPLTPPWAGLLKKYLSEEVTEKVLLAPHTTFGL
                     GGPADFFYAPRTLTEVARAVQLAKDEGIPYYLMAGGSNLVIADRGFRGLVIKITCAEL
                     EADNHTISAQAGISLGKVVDTAYEMGWAGIETLTGIPGSFGGAIYGNAGAYGRSISDV
                     LESAVVYDSKGQVKIVGPDYFGFRYRHSDLKVTKEVVLSARIRLTPGDKPKLKAKMEE
                     ILATRHAKHPVDLGSAGSYFKNIEDPQAEFGKVPAGLLLDRVGAKNIRIGNAGVYRRH
                     ANIIVNYGGALTSDVLRVAELMREKVKRAQGITLEEEIQLVGDFDEPPAGILKAA"
     gene            complement(14379..16034)
                     /locus_tag="AC16GM_04330"
     CDS             complement(14379..16034)
                     /locus_tag="AC16GM_04330"
                     /inference="COORDINATES: protein motif:HMM:NF028259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit"
                     /protein_id="HIR2922938.1"
                     /translation="MLRLLLKVRFKSYLNQFRRVSWGKRIGALALLLAYLYVGVMLSL
                     LFVALFKLFQRPELAVLGLDYHLLGVLFLAVLALLLVSGNSFTIYTIFLSPDLPLLST
                     FPLSRRTIFTGKFLEGTALNALIALVFLWPVMLGFGLAHRVTFVYYPVALAGLFLLVL
                     VPTGLSVFTALFFIRILPVTRIKEIMAAAGSILGIAIWVGSQLVSQRAEHAFSGTQAY
                     KTVESLRPLLDRPWFDYLPSHWMAKTLCGIAGLDFPVLFPAFLTVTFATLFFFGCLLL
                     AERVYYRGLSALQSEAVRQRKRKKPRTIAIPLPDRVRSPLWAIAIKDLRTTFRNFNLL
                     INIAIFPLMVILMPVLMSQNQKMPSGIGHLVQYFLIFFLLIMAASQMGMRTVPLEGKG
                     FWLLLTSPLALEKILWSKLWSSLFLVMLIAVAGLVSLGLLRGVDVTFSLFTAVASLCA
                     AAGAASLGIWQGAAYANFDWTNPKRMIKGAGGITFVFINLGYIAVWAVIIGLTYLAMK
                     TLHLPVWLAAVVLALLGISVAWALTMLSIKRAAVSIAKREWEY"
     gene            complement(16009..16803)
                     /locus_tag="AC16GM_04335"
     CDS             complement(16009..16803)
                     /locus_tag="AC16GM_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2922939.1"
                     /translation="MLKAVDLTKQFGKLTAVDHLNLEVKKGEFFGFLGPNGAGKTTTI
                     KMMVGLMRPTSGRVEIGDFDLAAHPLEAKRLLGFVPDQPYLYDKLTVSEFFDFVASVF
                     QLDRRTAAWKKKSLIELFDLGEKEDELIGTLSHGTKQKVALGGALLHEPKVLLLDEPT
                     VGLDPRSARILKDILNELARTGCVVFMSTHIMEIAEAMCDRVGIIDHGKLVAVGNLDD
                     LRRKTKEVGATLEDLFLSLTGGSEYEEVKRFLSAPAPTYAPAAPQS"
     gene            complement(16828..19425)
                     /locus_tag="AC16GM_04340"
     CDS             complement(16828..19425)
                     /locus_tag="AC16GM_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2922940.1"
                     /translation="MIGQTISHYKISAKLGEGGMGVVYKAQDLRLDRTVAIKFLPEHL
                     TADPAARTRFVAEAKGASAINHQNITTVYDIDEADGKSFIVMEYLEGKSLKDLAQEGV
                     RINRVSDIGIQIAEGLQAAHRKEIVHRDIKSENILVTKDSVVKIMDFGLAKLKGPSHL
                     TQTGATIGTVAYMSPEQTRGEEVDHRSDLFSLGVVLYELLTGKLPFSGEHQAAIIYSI
                     CHEAPEPLARYKAGIPEELQRVVTKTLQKDRNLRYQSAADLGADLKALRKVEEGTGAP
                     VSPPEPVRRQKTWVAVAIALIFVVIAGYLLFSRFFGPKGEKSEARRKMLAVLPFENLG
                     SPEEEYFATGITEEITTRLAKIGGLGVISHTSARKYKKTEKTLKQIGAELGADYILEG
                     NIRWAKSDGASRIRINPQLIQVSDDSHLWAENYDAVLNDVFELQSSIAEKVAAALDVA
                     LLEPERRVLAAKPTENLEAYDFYLRGNDQFNRTSMVGVGSEKYLRIAFQMYERALDLD
                     SNFALAHCQFSRVNTELYWHYDRKDQYLKNANEGVEKAFKLSSGLPEAYLALGSLHYH
                     AGEYEKALEEFAIAQKGLPNNPDLLKEIGFVQRRKGKWDEAIASFKKAAEVDPARSDL
                     PIALGGTYARLRKFTEAASYYQRAILLDPGVSGQYVILMYVYVRQDGNTKKARRTLEE
                     ATGKVDAAEFNRGWVWLDISDGNYPQALNRLNGPEAYDNDSVDYYLDKAKIYGLIAQP
                     KPARACYDSARGHLEKQLRTEFENEALYSQLGIAYAGLGRKEEAIREGKKAVELLPVS
                     RDAWSGPTRILSLARIYVMIGEYDAAVKELEYLLSIPSELTASLLRLDPVWAPLKNHP
                     RIQKLLAGK"
     gene            complement(19540..19734)
                     /locus_tag="AC16GM_04345"
     CDS             complement(19540..19734)
                     /locus_tag="AC16GM_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922941.1"
                     /translation="MMKDDSVARQLEALLNESDKCAHAIQKLIPKIGDYDLVRLLKKI
                     DADLADIRHNLAVTKRLAEK"
     gene            complement(19745..19963)
                     /locus_tag="AC16GM_04350"
     CDS             complement(19745..19963)
                     /locus_tag="AC16GM_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922942.1"
                     /translation="MEEIKGQQLGALLAEMLRETDKRAHGLHRVMHRVKDYDLQRRLE
                     KIEADLADIRHTFAVAKRLTEQCFGERA"
     gene            20298..20726
                     /locus_tag="AC16GM_04355"
     CDS             20298..20726
                     /locus_tag="AC16GM_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR2922943.1"
                     /translation="MAPKVKLFADYLASRGLKLTGERQKIVIEIFRTPRHFEPEELLI
                     DLRGRHLKASRATIYRTLELLMEAGLIRKVCLGSGRFSYEPVSEDRAHDHLICVRCGK
                     VVCFIDTEAEAAKKRISAEKGFELTGQCFQLFGYCPACRS"
     gene            20869..21963
                     /locus_tag="AC16GM_04360"
     CDS             20869..21963
                     /locus_tag="AC16GM_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922944.1"
                     /translation="MQRGILMACLCVSSGGLAAAQETSPYHFHGYGEMHFNRPKIGTM
                     SQGAGDEIDFHRMVFGFGYDFSEKIRLDFELDFEHAFTEPELEYAQVDFDLLPGLTLR
                     GGNLLIPVGPLNEFHEPPLFYSVERPYVQRSIIPTTWQENGAGVVGRVLDNRLAYRAY
                     AVSGLDGAKITSLDGLHDVSSKGAEAKANDFAGTGRVEFTPIRELTVAASGYFGGADQ
                     DEPGLGKVEVDIAEGDFHFQIQDIDLTGVFVRTKITGARQLSLAAGETIGKAMQGWYG
                     EAAVHTLKRLLPETSHDLVLFGRFEKFDTNHEVPAGFVQNLKADREIFTFGLAYYPID
                     RVTVKSDLELWKDGTDDTVSRFNLGLAFMY"
     gene            21982..22533
                     /locus_tag="AC16GM_04365"
     CDS             21982..22533
                     /locus_tag="AC16GM_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922945.1"
                     /translation="MKTSQTFASFLGLSLFLSSLAWAGEKKDIAQPFARLTVKFNGCA
                     SCAGCRSAMRQVAQTKSARHARIQIGDDRSLSATYPKATNIPMRELPAALGKSGLHQF
                     EVAEVTLETKGTVTEKDGQFFLILPETGQCLLLSGLANAEDVTGQELALRGKVRNWQS
                     PEEQLVLDVQSAQSIASAASERK"
     gene            22541..22819
                     /locus_tag="AC16GM_04370"
     CDS             22541..22819
                     /locus_tag="AC16GM_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR2922946.1"
                     /translation="MPSATGEAKIPAPVRKNLAEFTPGQVGVVSEIHGEVELTLRMME
                     IGLVPGVRVRYLRAAPLGDPIQVEVEGFLLSLRKSEAEAVILHSSELP"
     gene            22816..24978
                     /gene="feoB"
                     /locus_tag="AC16GM_04375"
     CDS             22816..24978
                     /gene="feoB"
                     /locus_tag="AC16GM_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR2922947.1"
                     /translation="MTAQLTEKNVFTVALVGNPNCGKTTLFNALTGLRQKVANYPGVT
                     VEKKTGLWEIEGTTVEALDLPGSYSLVPRSPDEKMVADVLSGRRPDLPAPDAVICVVD
                     VTNLSRHLYLVGQVIELGRPTVVALTMVDEAERIGLTVDTAKLSARLGVPVVAVIAAR
                     RVGLDQLALAVKKGVFSQWRSPERFWRLLPAFENRVKAYAPEAAATELETVACVAGLH
                     TPACTEKLEKVANQIRSELGVNEETVRRYVVEARYRWQREVTREVVEKTGDPRVDWTK
                     RLDKIFLHSILGPLVLLVTLTLVFQSIFSWAQAPMNAIGYVTGTFLPSLTYKILAPGP
                     LSSLINDGIFAGVGAVVTFLPQILLLFFFLTFMEDSGYLARAAFLLDRLMSKVGLSGR
                     SFIPLLSSFACAIPGIMATRTVPHHRDRLATILVAPLMTCSARLVVYTLLIAAFIPVA
                     HVWGPFSLQGMTLLGLYLLGIFAAAGVAALFRRTILRGPTPPLLLELPPYRWPKPQTL
                     FLTLWDRAKIFLVSAGTIIFAISVLLWGLSSYPRWTSIESHLTAGQKILFSQPDGPQK
                     QAALEALNQKLAAERLEHSFAGYLGKAIAPVLQPLGFDWKIGVGILGSFAAREVFVST
                     MGVVYGVGSAAEGQTLTDRLKADRSPVTGTLVWTPLVAITLLVFYAFALQCMSTIAII
                     RRETNTWRWPAFAFAYMTVLAYGGALATRWIGQAFFGG"
     gene            25358..26875
                     /locus_tag="AC16GM_04380"
     CDS             25358..26875
                     /locus_tag="AC16GM_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2922948.1"
                     /translation="MEGRAALKRLSIFGLAAIFSLAFPFSSASSQTSLLYRFDGSALD
                     ELGYSVAEVGDVDGDGKADFIIGVPRADPNGLTDAGSAFVYSGATGALLYRVNGAADF
                     DFFGFSVAGAGDIDGDSRADFIAGAPFASPEGIFIAGSAFVYSGATGVLLYQKNGEPG
                     DRLGHSVAGGGDMDGDDRADFIVGAYFADPGGMVNAGSAYVYSGADGGLLYHKDGTAA
                     TDRFGQFVAVTGDLNGDGPADFIVGAPLTDPGGLADAGSAYVYSGADGHLLYRINGAS
                     AGDGLGVPVAGTGDLNGDGQAEFLVGATFADPNGLADAGSLSVYSGKDGSLLFQENGV
                     SAGGRLGFAVSGAGDLDGDGVPDFMAGAPFADPGGLEDAGSAFVFSGANGLLLFQKNG
                     TSGDQLGWAVAGSGDLNGNGSAGFIVSARYADPGGLVDAGSVFVYQCLPRGDVNNDGE
                     TDVMDIVGLIQHVVFDVSLNSLIAGDTNCDGLSEIVDIVLLIEYLVYGTPALCCL"
     gene            26972..>27861
                     /locus_tag="AC16GM_04385"
     CDS             26972..>27861
                     /locus_tag="AC16GM_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922949.1"
                     /translation="MKRLSIVSAGILLLCAGATREGIAGPFVDSVIVVSRVFSPGSTG
                     TIDILLHNDSILSGIGLPLVIKSETGGAFGRIDSIQRLPSLNPFGMTSVLKVNGVTPD
                     SVYMLSVDSGGGTVSPGSRRAGYRLYVTSNNNQGTFSLDSNRLSSANSLKFAHPPTQI
                     RIPVFIRGMEIIGGSLSLAATQVTLFKGKDSCVTVGGMLCDTTSQTLTLDSVRVQHSG
                     DPDLLCITAIGATNLVGKTLSAFSCATDSGNGFLRVCQAPTASIGDTGTCTITFFGTL
                     PSGAGAPARIVAFSAAISMAS"
BASE COUNT         5659 a         7764 c         8127 g         6311 t
ORIGIN      
        1 gcccgccaac ttggccaagc caaaatcaac gatcttcacg tgcccttgac tggtgacgat
       61 cacgttcgac ggtttgatat cccggtggac aatgccggcc tcgtgtgccc gttgcaagcc
      121 ttccgcaatc tggagagcga tagcaattgc ctcttcgatc ttcagtggtc cggcttctat
      181 ctttttcttc agcgtttcac cctcatagta ggccatgcaa atgaaggaac gtccgtcgtg
      241 cgtctcgtcg atctcgtgga cgaccgcaat gtttggatga tcgagtgcgg aagcggcctg
      301 cgcctcatgg acgaagcgca gcttggtttc ctcatcgcga gtcaagtcca atgggaggaa
      361 cttgagggcg acgggccttt tgagcttggt gtccagggcc ttgtagacga cccccatccc
      421 cccctcgccg agtttctcta cgattttgta gtgggagatg gtttggccga tcatttcgcc
      481 gccgattgtt ttaacttcgc caatcgtttt tgggcgtctt cgacttcagg aatgccggga
      541 tcggcgtttt tccagatgtc taaaaactcc tgatatttct ctatcgcctt ttggttccag
      601 cccgattttt cgtatgccaa gcccaaaaag taattggcct tcaccgacca tgttaaaaaa
      661 aagctccgga tttcatcata ccttgaaagt attttctcaa actcactcac cgcctccccc
      721 agccgccccg cttccagata ggcttccgca agcagatagt gtaccgggaa atccgtgtgg
      781 gctttgccgg cttttgctaa ctgggctaca gccaccggca ggtttccttt ggtcaattca
      841 atccagccag tggcaaccca gtataaccac atccgagtag tatcggtctt ttcgatatcc
      901 ttcttaaaag ttcgggcgac ctcttcggct ttgtcaattt cgccgctttt ggctagaaac
      961 gcggcataaa aacctcgaaa attggccgca tcggaaggaa aagccttatg ataaacctcc
     1021 atacactttt taacttcctc cagggccagc ttcagatttt tcttttcttc atagattctt
     1081 gctttgactg cgtgtttacg cggcaacgcc gttccttcgg cccgctcgaa ttgatcggcg
     1141 gctatccctt ggtctaaaac ttgcagggcc tcattgaacc ggccctgata aacgggaatt
     1201 tgggccagat gcaacctacc ctccgagcga atatctttct cactgctggc ggccagcgcc
     1261 tgaaaagagc tttccgcccg aacgtattcc cttttgaaaa ggtacagata ccccaattta
     1321 gtcagagacg aatagaaatc cggctttttt tccagggcct tccggtagga ctcaatcgcc
     1381 tcgtccaatt tcccattgta ggcgtatagg tcgccccggg tgtcgtaagg attggcttcc
     1441 tccggcgcca gggaaatata tttatttatc gcccagatgg atttctcaaa atctcccaag
     1501 tcattgtaag cataggccag caggttatac gcggccttta caagggggtc tatttcaatc
     1561 gctttggtca aaggcccaac cgctttttcc ggctgctgaa gcgcgttcgc atatgtggcc
     1621 cccaaactga tatacgcgtc tttttcttga gggtaatcgg ccgctatctt ctcccactct
     1681 tttatggcca gcggaaaatt accggccaaa cgcgcctcca aagctcttat gtagtgcctc
     1741 tccatctcgc tgaccttgtt cgagtatttc acggcctggg ctatcgcttt tttggcatcc
     1801 ggattctgga gtcgggtgag aacgcgggcc aaatagaaat aggccatggt aaaggtggaa
     1861 tccagctcga tggctttcct gaaacttttt accgcctccg gatcatacaa tttttcccag
     1921 taatcaacgc cttccaagta gtagcgatag gcctccgggg aatgagtggt tgcatccgcc
     1981 accggcttgt ccggttcctg ggccgcttga gtcggcaagg ctaaatcttt ctttatctcc
     2041 gctgaaagcc ggtccaccac ggcaaaaata tcctgccccg cttctccggt aatgcgttcg
     2101 ctggcccgca ctttgcctga agcagcatcg gctatatccg aaactaaaac gatattcggt
     2161 ttagtctgca aaacgctgcc ggtcaaaacc catttcaccc cggctctttg agccacttcc
     2221 gaggccacgc ttttgtctat ggatttcagg ttttccttgc cgacgagttt cagaatatcg
     2281 tacagccgct gccggctgac cacctgcagg tactgggatt cggaaaggtc ggtgatgagc
     2341 aatgcggtga tcatttgaac aatccggtcc ctatcctccg gatccgccac gttgtcgaag
     2401 tacatcaccg ccagggagtt ctcggccgcg gtcgccttct tttccgagcc gatctcaatt
     2461 ttaaacggat tgagaatgaa gaagaggaag gcggcggcaa aaacgagcga ggcgggtacc
     2521 agaatgggga ggagtttctt tttcggtctg gccgccgcgg cttcaggtgg aatctggctg
     2581 gccttgacgt actccagctg ctttttctcc cttcgcaaat cggccaggag gtcgtcgatg
     2641 tgctggtagc gctcctcttt gtccttcgct aaagtcttgg cgacgatgcg ctccaattcg
     2701 ggggaaactt tgctgttgta tctggcaacc ggctgaggtt gttcgttgag gatggagaag
     2761 atgatggcgg cctggtgctc gcccggaaag gggagtttcc cggtcagaag ctcgtacagc
     2821 accactccga aggagaaaat atcggagcgc tgatcaattt cttcccccga ggcctgctcc
     2881 ggggacatgt aggccgctgt ccctaaggtt gaccccgccc gggtcaaccg gctggtgcct
     2941 ttcagcaggg ctaagccgaa atccatgagc ttgaccagcc cctcccgggt gaccatgatg
     3001 ttctccgact tgatgtcccg gtgcaccact tcctttttgt gagccgccgt taacccctcg
     3061 cagacctggc tggcaatctc cagaatcttc ttgagcggca aggtttcctt ttccaccacc
     3121 tgtttgagtg ttttcccctc cacgtactcc atggcgatga aaatcccctc gggcgtctcc
     3181 tcgatgccgt agatggtggt gatgttgggg tgattgagag ccgaggccga ctgcgcttcg
     3241 tgcaggaagc gcgccttctc gtcgggacta tccgcaacgt gcggcggcag aaacttcacc
     3301 gccaccggcc ggtggagttt gaggtccagg gctttgtaga ccacccccat ccccccttca
     3361 ccgagtttct ctacgatttt gtagtgggag acaattttcc caagcataag ggataaaagt
     3421 aggatagttt agtgcccaaa gcaacatgac tgggccacct gagttccccg cagcagaaaa
     3481 ttccggtgag tcaggatttg aatttggctt tcaatttctg ggctacgatg ggcgggacaa
     3541 aagcggtgat atcgcctgag tggcgggcga cgtctttgat cacgttggaa gagaggtagg
     3601 tgaactgctc agacggcatc aggaacaccg tttcggcctt gggggctaaa cgccggttca
     3661 tcagggcgat ctggaattca aattcaaaat cggaaaccgc gcgcaggccg cgcagaattg
     3721 ccaccgcccc ggccttggtg gtaagctcgg cggtcagacc ggcgaaggtc atcaccttga
     3781 cgccggaaag agacgccacc gtcgctttga ccatttccag ccgctcttcc agagtgaaga
     3841 gcggatgctt ttccggattt tccgcaatgg cgacaaccaa ctcgtcgaac aactccagcg
     3901 cccgcttgat caagtcgcga tggccgttgg tgatggggtc gaacgtgccg gggtaaatgg
     3961 ctattttcac ggatacctcc cgggaaggcc gccccggatc gccggatccg gaacctgttc
     4021 cttttttccg gctggcagag cggaattcat gccgctccgg gtcggtactg ataaattgaa
     4081 agtgcggtgt ctccgaaaac gcggtgcttg accagcgcca gcgtttcgaa ctgttccggc
     4141 aagacctctc ttttatcgtg ctcacagacg agaaagcctt ccggtttcag cacggagttt
     4201 ccagagagca ggatgactaa cttccggcac agaccttccc ggtagggcgg gtcggccagg
     4261 acgagatcga acgcctgacc ctggtgcgcc agacgctcca cgacggtctg aacatctccc
     4321 cgcaggacgg tgattttctc cgcgactccc agaagggcgg cattgcgttt gatcagattg
     4381 acggcgtcaa aacgcttctc cacaaaggtt acccccgcgg cgccccgcga gagcgcttcc
     4441 agaccgaggt ttcccgatcc ggcatagagg tcgagcaccc gggcacagac gaccgtcgcc
     4501 cccaggatgt tgaaaatggc acctttggtc ttatcgctgg aatagcgcag gctccgcctc
     4561 tggcggggtt cggtgaagag tttccttttc ttggcgaaac cggcaatgat gcgcatcgtc
     4621 aaaaacccgc agccatttcc ttccgtctga aaatatagcg cccccttagg gaaatttcca
     4681 ctcaaagaga aaggctcgtt ttctccgagc acggtcgttt accttcggca gtctaggacc
     4741 ggctcaagga taaatgggtc aacgaacctt gggggaaatg gaggaaatca atggggagcc
     4801 agcgccaagc ccaaggccac gctatagcga tggctttggg aaagaaagtc ttctctcttg
     4861 ggcttgagct cggatgacca atcacaggcc gctaagaggt tggccggttc caccggaagg
     4921 gagagggccg tggctattgc cttttgtatt tccgcatcgc tcgcgccggg gccggagaga
     4981 gcgattttcc cgttttggag gtcggggcct tgactgcgga ggagcgtggc cacttcctca
     5041 gccagggcgc gcgtcaactc gcttttgagg agaacgaagc cctcgttcaa ctgccgggga
     5101 ggcgtgtcga aaagtccggg cggagtcgtc agcgcttcga tataatccag ttcgccgtct
     5161 ttccccacgg cgaggttcgt cacctcttcg ccgacgtcga gcagaaccgt cgttcccgcc
     5221 aaaccggcag agagcgtctg gtgcgccgcc cagagcgcca gaggcgtggc caggaagacc
     5281 tggcactcca agccggcttc gtccaggatg cgctgggtca tctccaccgg ctcgcgccgc
     5341 gtcaccaccg cttgcgccgt cgcccgctct ccggtttgga gcgctttttt ccagaaatcg
     5401 atcaccaggc cttcgggagc agcgagcaga ccttgtcgca tctcccattc cggccagtgg
     5461 gggtaaagcc cggccagctc ggccggatag gtcacctttc ggttgagcca ggccggtcgc
     5521 tcgagattca aacgcaactc cccgttgcgc caggctgcgg tatttccttt ccgggccacc
     5581 agcgctttca ccacggccgt gaccggatct tttttggttt tggcctgttc ggcaccgaga
     5641 tcggtgatgg ttctctggct gccggaggtc tccaccacgg cgtaggtcac tgccgccccg
     5701 ccggtggcga tgccgatggt tttctgctgg aaccgggtca tcctactatt tagtttcgac
     5761 cagtggggcg atttgctgta atttctttcg gccgattccc ggcacccgca agagatcccc
     5821 ggccttctgg aacgggccgt acttctgacg cgtggtgatg atccgctgag ccagaactgg
     5881 tccgatgccc ggcagagcgg ccagttgctc cccggtggcg gtattcaggt cgactttctg
     5941 gcccagttga aattggggaa ggagggtccc gctggtttcg ggagccaaag aggcgggagg
     6001 cccaggggtg atggacttgg tgcggaagat caactccggc tcaaggcggg tcttctcccg
     6061 cttctgcagc cagaggcccg cgccgatcaa aaacgttacc gcgaggacca gtacggcaat
     6121 gcgctgcaag cgagtgaacc agagggtcac ggttgcctca tattcctcaa ggtgcggtta
     6181 tggtatgccc gtctccccaa tgggtcaagc aggtcagcgg ctgtagatga aggcggccag
     6241 cacccgcccg atggcggcca ggctttgcgc agagcattta tcgggcgtgt caccggtggt
     6301 atgccaccag ggatagtcga aatcaatcac gtcgatggcc ggaatgccgg cccagagcag
     6361 ctggaggtgg tcatcttcga ctgagtattt cgcggaatcc ctgaaggttg tgactcccag
     6421 ttcccgggct ttccgccaga ctttttccac cacctccggc gcataccgca ctgagttttg
     6481 ctcccggtag atggccaggt cgcggtcgcc gaccatgtcc aggagaatgg ctaactccgc
     6541 tcgttgtggc gggaggtggc gtgcgaagta gcgcgacccc agaaagtacc aatcgagttc
     6601 tccctcggga ccgaaatctt ccccgtcaaa aaaaatgagg tcgatgccca cctccggcgg
     6661 gtgttcgtgc aaaaggcgcg ccagttccag caggacggcc acacccgagg cgccgtcgtt
     6721 ggccccgccg gtcggttcgt tccgcttggt gctgtcggga tcgcgttcgg cccggggccg
     6781 ggtatcccag tgcgcgcaga gccagaggcg ctcccttttt tccggagcga aacgggcgat
     6841 aacgttggtc aacctgatct cgcttttacc ccgggagaga gagaattgtt gggtgtccac
     6901 ctgagcggca aagggttgca acgtgtcgag caggaagact aacccgcgct cgtgcgtccc
     6961 ggtgccgggc actttcggcc ctaaagcgta aaagtcattc agcgtccggt acgcccgtct
     7021 cccgtcgaac cccggcaggg atcgtgaaac acagccccca ctccaaaaga ggaagcaccc
     7081 gaaaagcaga ctctgccaga gaatctctct cgaacggggc cggtgaagca cagcttaaaa
     7141 gcgcagctcc atgtagaagt tcaattctcg cgtgtggctc ttgcgcttgc ggatcaggtc
     7201 gttggtgtcg ttccagatca cctgcaggcc gccattcacc tgcgaggaaa aggagtagtt
     7261 gccggtcagg cctaaattga agatcgagtt gtcggtgaga atggccagcg gccggccccc
     7321 cggagcctgc ttttgcgtgt cgcgtctttt ggtgaccgcg aggtcgagcc tcagggtcga
     7381 ctgcagccgg atgccccgca gtaaggggag ttggatcccg tgcggggctc gaaaggaata
     7441 gctggtggaa agggtgaatc cccgggaagt gttctcggta cgcgtgttgg ccgacccgat
     7501 actcaagtcc tgacttttgg cggtgctctg gttggactgc agggtcgtgc tcataccgtt
     7561 tttccagttc agggtcaagc cggccaaagg ctggaaggtg gcggtggtgg tcctggcagt
     7621 gggatgcctg cggttcaggt ccaaccctgc gtcggatttg aaggcataat tgctctgcag
     7681 ttgggagctg gagatcaatt tcttcagcag gccgaaacgg tcaacgttcg tcatgctgaa
     7741 acgcaggtcc gggaagacgc ggctggtgct tctggtgttg ctggtctggg tctggctggt
     7801 gccgacccgc cggctgtagg caacgtccag gtcgaagccg ccgcccagtt ttaggccgct
     7861 ggtggcggag tagttatccg caaaactgga ttggtcgccc agactggttg tgctgaagcc
     7921 ggtcttttcg ctgaagccaa accggaacgt ctgctgcgga ctgccgggca gcgcctggcg
     7981 ggacaggttg cgatccttct tgtaggacaa gcggaccgga tcaatgatct gcaacccgcg
     8041 gtacagcgac cgggagacga ggcgtagcgg ggagaagccc ggccctttcg gagcgccggc
     8101 tttgaccgag tcggtgctgt cggccgagag ggtgcggccc gcccgctcga ggctgtcgcg
     8161 gttcgctccc gccggcgggc tgacgctctg cggaacggtg gcgggttttt tcggctgttg
     8221 agccggcttg ccgaaactga aatgtcccag agtcgcctga tagaggttcg gcaaattgac
     8281 cagcgaggag gcgccgtagc cggaagtcag ggtcaccgtg cgtccataga ccgtctgctg
     8341 cgggttggcg ttttccgcat agttggcgct gtaggacagc gaattgtcca tgaaactgaa
     8401 aattctcggc gtgtagttgg caccaaaggc ctgggtgcgg ctggtttcaa agccaaaacg
     8461 cgcggtcttc gggttcaggg agaaatgcaa gttgtccgga ttggccaggt cgcgctgggt
     8521 ggtgccgtcc agggtaacgc tcagcgggga aaacggctgg aaacgcgtcg accagccccc
     8581 tgaaaaattg cgggtaaaga ccgtggtcgg gcgctgactg gagttgacct gctcggtcag
     8641 gacccgggag agcgacccgc gcagggtgaa cgatgacggc aggagctgga gtttggattt
     8701 ggcgatggac ctcggaacca aaagccactt cgaccaggcc agccagggaa tgccgagctg
     8761 ggtgcggggc gacaggtcgt agttcccgga gacgtcgtag cggctctgat cgtttttggg
     8821 caaggtcggg gtatggctct ggctgcgacc gtaggcatag gtaaattgcc ccagccggtt
     8881 caaggtcaag ttcaccagcc agtttttcgt ctcgatccgg aattggtggg agagcgtgag
     8941 gttaaagtcc tggttggtgg tcgattcctc ggcccgcagg gccggggcga gcacgatgtc
     9001 cgaaccgttc ttcagccgcg gcaccgagct cccgttgcgg tagttaatgc ttaagggaag
     9061 ggtggccccc cagcgcggcg gtaaaaactt ctgcagggcc agctgcgtcg acacggttaa
     9121 attgttctgg gtactggaga aacggtacga cgaggcggcc agactgccgc tccgatcgcc
     9181 ggaggtcagg ttgcggaaga acgcgtcgcg gtggaggtag tcgaccttga ggtcgatgaa
     9241 gtcggaaaac tgggcgccgg cggaaaaccg ggccgccagg ccggcatccc ggcggacgtc
     9301 ggttacgcgg agttcgtcgg cccagatctc gcctgaagcg ggcgggccgc ccgcggtgtc
     9361 tgcgttggtc actcccatgg tgaaccagcg caccgagccc agggtaggat tcccccggat
     9421 ccggtagttg ccggtcaacg tgtcgggaac ctgcgctccc ccggaggggt gatttttcaa
     9481 aagaagccct ttgatttcgg tcatccggtc aaaatcaatc accacttcgt tgcggctgtc
     9541 ccagccggga tagactttgg tccggtactc gtagtagttg ttcgtgtcgg cgttggtgcc
     9601 cgatccgaag cgcaggaaaa agaggagttc tccagggccg gacggaccgt ggacgaacat
     9661 gtgcattctc cggtagccgg aataatcttc ggcggtgccc aggatcgtcc gctgggccag
     9721 accggtgtgt ccgggcccga ggttttcata ctgcagcacc aacgactgct cctgctggcg
     9781 cacgttggtg atccggtcaa tggtttcggt gacgccgggc gggatttggt agtttttgtt
     9841 ctcctgggag ttgatgaccg ccacccggaa tttttcgtcg ggggagatac tgtctttcgg
     9901 tgtaagcccc agttcccgcc agcggttccc gaccacggcc tgcgtggcaa tggcaaagcg
     9961 ggccgtatcc cgaactccgg tcacccagaa ccggacgtac tcgatcgagt tccagtccgg
    10021 acggccctct ttccggtaga tgagactatc ccgcaaggga atgcggtaga gccgccagcg
    10081 agttccgtag gatgtccctg taaaaaagtc gttgccaggt tgggaccaga agcgtcccag
    10141 ggtccccgga actatgaaag gggaggtggt atcagctaaa tcgagtgtga actcaaagta
    10201 ttcgttgtgc gtctctaacg caccgtcacc attgagatct tcggtatcgg gccggtcgcg
    10261 ccggccaggg tcaaagcgat ttccttcagt tccgttgatt tgatcgggtc tgctatgcca
    10321 gttgtccccg ccgggatcac cgatcgttcc tccgaacgcc ggtggttcgt tcccatccgg
    10381 ctgcccatcc aacccgatat cttcacccgg ggtcaggatg ttgtcccgaa tgccgttgac
    10441 cgtgctgtct tcggtgttga gtataccgtt gctgtccaaa tcctcctgga tttttccaaa
    10501 gtcgaaatgg agaatgcccg aatcgccggt ggcccaaact tccaggtttt gagcctgcga
    10561 gaggttcaca ccgccgccaa agaaggagcg cataatgcca ccccaggaag actcaggcgg
    10621 cgccgcaccg gtgtcctttg gtacaaagac aaatttcagt acatcagtgc gttcatcgac
    10681 cgtgcgggta tcccggtcgg gccagatatc tttgactcgg aagcgttgtt cccaatcctg
    10741 gccgtccggg ttgtaccagt gtaaacggcc gcggctggag ggacctttgc cgaccggcgg
    10801 gctggcggcg gtccaccctt cacggaaaat gcccagatcg accgtttcct tgctcccctc
    10861 aaaatcgtcc aagtaggcat tctgtctggt gttcggattg ggaaaactcc gggcaatttc
    10921 tccggagaga ttgacggtag aaggcgcggc cgcttccacc agaggcaagg cattgaccgc
    10981 ccgggtgagc cagggggcct ggaaacggta cgaggcgtcg gcatcccaga gcagggtgcg
    11041 gttcggctcc tggccgattt tgggtttctg gtcgttggag gtttcgttgc ggaacaagac
    11101 cgtcgtgcct gcccggaagt tgtcgcttaa ctggtagtcg ccccgcaggc cgaagagggc
    11161 ctttttctcc gtggagagga agggggagta gtcgaagttg atgctcaaat cggcattggg
    11221 gttcagcacc tcctcgttca agaacgaaat ttgccccaac tggtagtcga tattgtaatc
    11281 cttcccgcgg acgagccggc ggttgttcag gaggacctct tcggaattct ccaggatgtt
    11341 cacgcggccc agggagaaat ctttttgacg gcccgtgaga cttttgattt cgaggtagta
    11401 ctgggtgttc cgctggagga catcagggac attgatggaa tcgtaaatgg ccgcgatgcg
    11461 ggtgcgcaga ctggtggcgg tgtcccccgc atagctcacg tcggaattga aaggatagcg
    11521 ctccggaaag aagaggtgcc cgcgcctgag gtcaagaacc tcttcgcgct tatccacctt
    11581 gttgtctatg agcctcaccc ccagggcatt gaactggtcg atccccgtaa cctgaaggta
    11641 gttcgctagg cccggatcgt gttcctgccg ggcgggattc gattcatcgc cctggctgcc
    11701 tttgaagatc tgcacattca aaccgtccca gtcgatgttt ctcgcgccca ggttgtagac
    11761 gttcttccac tcgtaactat aggtgacgtc ggtcggacgg ctcccgcctc gccgcctttt
    11821 aataagcttc aatttgagaa tgctgtcgca ggcggcaagg tcgatacagc cgtattctat
    11881 cgttgtatcc ggattactcc gcttgatttt cagataaacg ccgatgatgt cagattcctg
    11941 caactgaggt tcgaaataga ccacaaaggg ccgcaggaga gaattgacga agtaattgga
    12001 atcaggcttc aacctctcga agtacccggt ttcccgctcc agagtgtcta cgtcgggacg
    12061 gttggggtcg ttggggtcaa cgtaacagat acccgggagc gcgatactcc cggtgccggg
    12121 agcagtgctc gacttccacc tgtagacttc cagttggatg atgctatctc tggcgggatc
    12181 aaattcattt accccgcgct gcgggtcggc cttggtgttc cggcccaggt cgtagtaggc
    12241 gtagcggagg tattgataat cgcgcaggta aatggtggct ccctgcgcac cggcatggaa
    12301 gttaaacctt tgggagttgc ttttgtcctg actcgtaatc atagtcaggt ccagcccgcc
    12361 cagtttggcc tgagttttga ttccgaacaa cccctgcttc tgttccgaat agccgacgaa
    12421 ctgcgtgccg ggcagactca agttcgtatt gcccgcttcg atcgagcgga tgacctcgtc
    12481 ttcatcccct ttgtagcgca gttgaatgcg gtcggccagg ttggcctgat cgaagacatt
    12541 ctggttggag ttctggcgca ggtcaatcgt aatctttgac cccaccgtgc cggaaatgtt
    12601 gaactggtag tcctgtttca tctggatggt ggggaacttg ctggcccggt aggtggcggt
    12661 ccgctccaga ccatccaccc agttggaacg cccgcccatg gtgatccgcc ggtagccgga
    12721 gactttcagc ccggcagagc cctcgccgat gatgttcgcc accgctttgg gcggcttcca
    12781 gggaatctgg aaagtggtca gaccgcccgc cttctggctt tccggttttc ggatggaagt
    12841 cgctttcaat ttgccccact cgtccagata gctcgtctgg ctgcgccagt cgcggtaggc
    12901 ggagaggtcc aacaggagcg gatcgtccgg tgagccgttg gagaggcgta tggcgccgcc
    12961 ggacgaatcc agagcagccg tatgcggctt taagctcgcc ggcagggaaa ggagcgggac
    13021 tgaatccaaa caccagcaga ggcccgtccg gggggtcggt ccttgggacg ggacagagcc
    13081 atcgacggcg aggtccaggc gcagtccctg gccaaaacag aggaggggcc accccaagaa
    13141 aaacaaaaac gccaaccacg cgctttggcg ccgcctgttg gcgaagattt tggtcacgtt
    13201 cggcaaacga ctaacccttt ttagtcgatc gtaacttttc gccttcggtc tttatttcct
    13261 cctttcgctc gggttgactg tcgccatcag gccgccttga ggatacccgc gggaggctcg
    13321 tcaaaatcac cgacgagttg aatttcctcc tccagagtaa tcccctgcgc ccgtttcacc
    13381 ttttcccgca tcagttcggc cactctgagc acgtcactgg tcaaggcgcc gccgtaattc
    13441 acgatgatgt tcgcatgccg ccggtagacc ccggcattgc cgatgcgtat attttttgcg
    13501 ccaactcggt ccaagagcaa tccggccggg accttgccga actcggcttg cgggtcctca
    13561 atgttcttaa aataactccc cgccgaaccg aggtcaaccg gatgtttggc gtgtctggtc
    13621 gccagaattt cctccatctt ggctttcaat tttggtttgt ccccgggcgt caaccggatc
    13681 cgcgccgaca gcaccacttc tttggtcacc ttcaggtcac tatgccggta gcggaagccg
    13741 aagtagtcgg gcccgactat tttcacctgc cctttactat cgtacaccac tgcgctttcc
    13801 agaacgtcgc tgatcgaccg gccgtaggcg ccggcatttc cgtaaatggc accgccaaaa
    13861 ctgcccggaa ttcccgttaa ggtctcgatg ccggcccagc ccatttcgta ggccgtatcg
    13921 accactttgc cgagcgaaat tccggcctgg gcgctgatgg tatgattgtc ggcttctaac
    13981 tccgcacagg ttatctttat caccaatccc cggaagcccc ggtcggcaat caccagattg
    14041 ctcccgccgg ccataagata gtagggaatc ccctcatctt tcgcaagttg aacggcgcga
    14101 gcgacttcag tcaaagtccg gggtgcgtag aagaagtcgg ccgggccgcc caggccaaag
    14161 gtggtatgag gggctaagag taccttttcg gttacctcct cacttaaata ctttttcagc
    14221 agccccgccc acggcggggt cagaggtacg tctctttggg taccttcctg cagtttcaac
    14281 atataatata cccggaatca ggcgcctgtc aaggattttc gtcgtttttc tcgctcgtgc
    14341 ctcagggtga gcgctgccta aactaccgta tcaaatgcct aatattccca ctctcttttg
    14401 gctatcgaca cggccgcccg ctttattgag agcatagtca aggcccaggc gactgagatg
    14461 cccaggagag ccaggacgac cgcggccagc cagaccggca ggtggagggt tttcatcgcc
    14521 agataggtta aaccgattat cacggcccag accgctatat aacccagatt gatgaagacg
    14581 aaagttatcc ccccggctcc tttaatcatc cgcttggggt tcgtccagtc aaagttggcg
    14641 taggcagccc cctgccagat tcccagagac gcagccccgg ctgcggcgca gagggaagcc
    14701 acagcggtga aaagactaaa agtgacatcc acaccgcgga gcaacccaag ggaaaccagt
    14761 cccgccaccg caattagcat gacgagaaaa agggacgacc aaagtttact ccagaggatt
    14821 ttttccaatg cgagggggct ggttaaaagg agccagaacc ctttgccttc taggggaaca
    14881 gtacgcatgc ccatctggct ggcagccata atcagcagga agaaaatcaa aaaatactgc
    14941 acgaggtggc cgattcccga aggcattttc tggttttggc tcatgagcac cggcatcaag
    15001 atgaccatca gggggaagat ggcgatgttg attaaaaggt tgaagttgcg gaaagtcgtc
    15061 cggagatctt taatggcaat agcccacaac gggctgcgta cccggtcagg aagcgggatt
    15121 gcaatcgttc ggggtttttt gcgcttcctt tgacggactg cctctgactg caacgccgat
    15181 aacccgcggt agtagacccg ctccgccaga agcaggcaac cgaaaaagaa gagcgtcgca
    15241 aaggtcactg tcaaaaaagc ggggaaaagc accgggaaat cgaggccggc gattccgcag
    15301 agcgtcttgg ccatccagtg ggatggcagg tagtcgaacc agggccggtc cagaagcggc
    15361 ctgagcgatt caaccgtctt gtaggcctgt gtgccggaaa aggcgtgctc cgcacgctga
    15421 gacaccaact gggaaccgac ccaaattgca attcccaaaa ttgaccccgc ggcggccatg
    15481 atttctttga tgcgcgtgac cggcagtatc cggatgaaaa agagcgcggt gaataccgag
    15541 aggccggtcg gaaccagcac gagcaggaag agccccgcga gcgcaaccgg atagtaaacg
    15601 aacgttaccc tgtgcgccag gccgaagccc agcatcaccg gccagaggaa aaccagggcg
    15661 atcagggcat tcaaggcggt accttcgaga aatttgccgg tgaaaatcgt gcggcgcgac
    15721 aaggggaagg tggaaagcag gggcaggtcg ggggaaagga aaatggtgta aatcgtaaat
    15781 gagttgccgc tcaccaatag gagagccagc actgcgagga acagtacgcc caacaggtgg
    15841 tagtcgagcc ccagaacggc cagctccgga cgctggaaga gcttgaacaa ggccacgaag
    15901 agaagagaga gcatcacgcc cacgtacagg taggcgagca ggagggccaa agcgccaatc
    15961 cttttgcccc aggaaacgcg gcggaattga ttgaggtaac ttttgaatct aactttgagg
    16021 agcagccgga gcataagtcg gggcgggggc ggaaaggaag cgcttcactt cctcatactc
    16081 agagccgccc gtaagggaga ggaacaaatc ttctaacgtg gcacctactt ctttggtctt
    16141 gcggcgcagg tcgtctaagt tgccgaccgc cactaacttc ccgtggtcaa tgatgcccac
    16201 ccggtcgcac atcgcctcgg caatttccat gatgtgggtg gacatgaaga cgacacagcc
    16261 ggtacgggcc agttcgttca aaatgtcctt taagatgcgg gccgagcggg ggtcgagccc
    16321 caccgtcggc tcatccaaaa ggagcacctt cggctcgtgc aggagcgcgc cacccaaggc
    16381 cactttctgc ttggtaccgt gagagagggt gccgatcaac tcgtcttctt tctcccccag
    16441 gtcgaagagt tcaatgagcg acttcttctt ccaggcagcg gtccgacggt cgagttggaa
    16501 gaccgaagcg acaaaatcga aaaactcgct cacggttagt ttgtcgtaca gatagggttg
    16561 atcgggcacg aaccccagga ggcgtttggc ctccagagga tgagctgcca gatcaaagtc
    16621 acctatttcg acgcggccag aagtcggtcg catcaatccc accatcattt tgatggtcgt
    16681 ggttttcccg gcgccgttcg gccccagaaa gccgaagaac tcgccttttt tcacctccag
    16741 gttcagatga tcgacggcgg tgagtttccc gaactgcttg gtcaggtcaa cggctttgag
    16801 cataggtggg agaagtggat ttactcttca tttccctgcc aaaagtttct gaattctagg
    16861 gtgatttttc aaaggcgccc agacggggtc aagccgaagc aaggaggcgg ttagttcact
    16921 cggtatggac agcagatatt caagttcctt tacggcggca tcatattctc ctatcatcac
    16981 gtaaatacgg gcgagagata gaatccgggt aggaccactc caagcatccc ggcttaccgg
    17041 caatagctcc accgcttttt taccttcgcg gatcgcctcc tctttgcgtc ccagtccggc
    17101 ataagcaatt cccaattgac tgtaaagagc ttcattctca aactctgttc gcaattgttt
    17161 ttccagatga ccccgggcgg aatcgtagca ggcgcgcgct ggcttcggtt gggcgattaa
    17221 gccgtatatt ttggctttgt ccaaatagta gtccaccgaa tcgttgtcat acgcttccgg
    17281 tccgttaagc cggttcaagg cctgcggata attgccatca ctaatatcca gccaaaccca
    17341 tcctctatta aattccgcgg catccacttt tcccgttgcc tcttcaagag tccttcgagc
    17401 ttttttggtg ttaccatctt gtctaacgta cacgtacatc agtattacat actggccgct
    17461 tacacccgga tcgagcaaaa ttgcgcgctg ataataggat gccgcctcgg taaatttgcg
    17521 caatcgagcg taggtcccgc cgagagcaat ggggaggtcg gaacgggcgg ggtccacctc
    17581 ggcggctttt ttaaagctgg ctattgcctc gtcccatttt cccttacgcc tttgcacaaa
    17641 tccaatctcc tttaaaaggt cgggattgtt gggcagcccc ttttgggcaa tggcgaactc
    17701 ttcgagcgct ttttcgtact cccctgcgtg gtagtgtaag gagccaagcg ccaaataggc
    17761 ttccggcagg ccggaagaaa gcttaaaggc cttttccacc ccttcattgg catttttaag
    17821 atactggtcc tttctgtcgt aatgccagta cagctcggta ttgacccttg aaaactggca
    17881 atgggccaag gcaaaattgg agtccaaatc gagcgcccgc tcatacattt gaaaggcgat
    17941 tctcaaatat ttttcagatc cgacccctac catggaagtg cgattgaact ggtcattgcc
    18001 gcgcaggtaa aagtcgtagg cctctaaatt ttctgtcggt ttggcggcca gcacgcgccg
    18061 ctctggctct aacagggcca catccaaagc ggccgccact ttctcggcga tgctggactg
    18121 cagttcgaaa acgtcgttta ataccgcgtc gtagttttcg gcccaaaggt gtgagtcgtc
    18181 ggaaacctgt atcagctgcg gatttatacg aatgcggctg gcgccgtccg atttagccca
    18241 gcgaatattt ccttccagaa tgtaatccgc ccccagttcg gcgccgattt gcttcaaagt
    18301 tttttcggtc tttttgtatt tacgggcact ggtgtgagaa atgacgccca acccgccgat
    18361 tttcgccaaa cgggtggtaa tctcctccgt tatgccagtc gcaaagtact cctcttccgg
    18421 cgaccccaaa ttctcgaacg gcaaaactgc cagcattttt cttcgagctt ccgatttttc
    18481 tccttttggg ccaaaaaagc gcgagaatag caaatatccc gcaataacga caaagattaa
    18541 agcaatcgcc acagcgaccc aagttttttg ccttcggact ggctctggag gtgataccgg
    18601 agcgccggtg ccctcctcca cctttctcag cgccttcagg tccgctccca ggtcagccgc
    18661 actctggtat ctaagattgc ggtccttctg aagcgtttta gtcacgaccc gctgcagttc
    18721 ttcgggaatg ccagctttat agcgcgctaa cggttccggc gcttcatgac agattgaata
    18781 gatgatggcc gcctggtgtt cgcccgaaaa aggcaacttg cccgttagta actcatacaa
    18841 aactacgcct aaagaaaaca gatcacttcg gtggtctact tcctcccccc gcgtctgctc
    18901 cggggacatg taggccaccg tgccgatggt ggcaccggtt tgcgtgagat gggacggccc
    18961 tttcagcttg gccaagccga agtccataat cttcaccacg ctgtctttgg tcaccagaat
    19021 gttctcggac ttgatgtccc ggtgcactat ctcttttctg tgggcggcct gcaacccctc
    19081 agcgatctga atcccaatat ccgaaacccg atttattctt actccttctt gcgccaggtc
    19141 tttcaaggat ttcccctcga ggtactccat gactatgaag gatttgccgt ccgcttcgtc
    19201 gatgtcatac accgtggtga tattctggtg gttaatggcc gaggcgcctt tggcttcggc
    19261 gacgaatcga gttctggcgg caggatcagc ggtgaggtgc tccggcaaga acttaatggc
    19321 cactgtgcgg tctaaccgga ggtcctgggc cttgtagacg acgcccattc ccccttcacc
    19381 gagtttggct gagattttgt agtgagagat cgtctggccg atcataattg cgttgtcttg
    19441 attttacgat tcaggagtag caccggcggg ggccggggga ctcaaaacca cctgtgggaa
    19501 gagcagacca gcagtggctt cacccatacc ggtgggagac tacttttccg ccaggcgttt
    19561 ggtcacggcc aggttgtggc ggatgtcggc caggtcggca tcaattttct ttaagaggcg
    19621 caccaggtca tagtcgccga tcttaggaat taacttctgg attgcgtgcg cacacttgtc
    19681 cgattcattg aggagggcct cgagttgccg ggccaccgag tcatctttca tcatgatagc
    19741 ccgcttacgc cctttccccg aagcactgtt ccgtcagccg cttggctacc gcaaaggtat
    19801 gccggatgtc agcgagatcg gcttcaatct tctccaaacg ccgctggagg tcgtagtctt
    19861 tcaccctgtg cattactcgg tgaaggccgt gggcgcgctt atccgtttcc ctgagcattt
    19921 cagccaacaa cgcgccgagt tgctggccct tgatttcttc catgatccac ctcactccac
    19981 tgctacatta taatacgcag tccgcgcgcc ccgcgcaagg ttatgtggtt tcaacgaagc
    20041 gaaaagcatt ctatttttct tctcgacaca ctttgggaac aacttcagat tctgtacagt
    20101 tctcgtccgc aatccgtgct gacttctggt cttcgacgcc tgttgcggcc aagagactgt
    20161 aaaaaaggtc agaaaactaa aggaggttgc cggagcgcag gagacaaaat tagtatgtga
    20221 aaaaagtctc aaacgagact tgacaggata aatcgagtct tttattattc cagtattgag
    20281 atagggtctc aaacactatg gcaccgaaag tgaaactctt cgctgattac ctggcatcca
    20341 ggggtctgaa gctgaccggg gagaggcaga agatcgttat tgaaatcttc cgaacgccgc
    20401 ggcattttga gccggaagag cttttgattg atttgcgcgg tcgccacctc aaggcctccc
    20461 gggcaaccat ctaccgcacg cttgagctcc tgatggaagc cggcctgatc cgcaaggtct
    20521 gcctcggttc cgggcgcttt tcgtacgagc cggtgtccga agacagggcg cacgaccacc
    20581 tcatctgcgt ccgctgcggc aaggtggtct gtttcatcga taccgaggcc gaggcggcaa
    20641 aaaaacggat cagcgccgag aagggatttg aactgaccgg ccagtgtttc cagttgttcg
    20701 ggtactgccc ggcctgccgg agctgacctt tggcctttta tttatgaacc tattgcaaat
    20761 gaatctcaat atcaaaaaga acggagcaag gttgtaattt ttttcacaag aaagatgact
    20821 tataagtcag gaatcaaaac gggaagccga aggaaaagag attcagtggt gcagcgtggg
    20881 attttgatgg cctgcctttg cgtgtcctcc ggagggctgg ctgcggctca agaaacttca
    20941 ccctaccact ttcacggata cggcgaaatg catttcaacc gacccaagat cgggacaatg
    21001 tcccagggag ccggagatga aattgacttc caccggatgg tcttcgggtt cggttacgat
    21061 ttttcagaga aaattcgcct cgacttcgaa ctcgattttg agcatgcttt taccgaaccg
    21121 gaattagagt acgcgcaggt tgattttgac ctcttgcctg ggttgaccct ccgcggcggc
    21181 aatctcctga ttccggtggg tcctttgaac gaattccacg agccgccgct tttctacagc
    21241 gtcgagcgac cctacgtcca gcgcagcatc atccccacca cctggcagga aaacggtgcg
    21301 ggtgtggtgg ggcgggtact ggataaccgg ctggcctatc gggcctacgc cgtctcgggg
    21361 ctggacggag ccaaaatcac ctctctggac gggctgcacg acgtcagcag caaaggggcg
    21421 gaggccaaag ccaatgattt cgctggaacc gggcgggtag aatttacgcc cattcgtgag
    21481 ctgaccgttg ccgcctcggg ctactttggc ggagccgatc aggatgagcc gggtctgggc
    21541 aaagtggagg tcgacatcgc cgagggcgac tttcactttc aaattcaaga catcgacctt
    21601 accggagttt ttgtacgtac gaaaatcacc ggagcacggc aactcagctt ggcggcaggc
    21661 gaaacgatcg gcaaggccat gcagggctgg tacggcgaag cggccgtcca cactttgaag
    21721 cgtttgctgc cggagacctc gcacgatttg gtgctcttcg gccggtttga aaagtttgac
    21781 accaaccacg aagtcccggc cggatttgtc caaaacctta aagccgaccg ggagatcttc
    21841 accttcggcc tggcgtacta tcccatcgac agggtcacgg tgaaaagtga cttggaactc
    21901 tggaaagacg gcaccgacga cacggtgagc cggttcaatc tcggcctggc cttcatgtac
    21961 tgagggaaag gagcagcgtt cttgaaaacg tctcaaacat tcgcaagttt cctggggttg
    22021 agcctgtttc tgtccagtct cgcttgggcc ggcgaaaaga aggatatagc tcagcccttt
    22081 gcccggctga ccgtgaagtt caacggctgc gcctcctgcg ccggctgccg gagcgccatg
    22141 cgccaggtgg cgcagaccaa atcagcgcgc catgcgcgta tccagatcgg ggacgatcgg
    22201 agcctctcgg ccacttaccc caaggcgacg aacatcccca tgcgggagtt accggcagcg
    22261 ctgggaaaaa gcggcctgca tcagtttgaa gtggccgagg tgaccctgga gaccaaaggc
    22321 accgtgaccg aaaaagacgg gcagtttttc ctcattctgc ccgaaacggg tcagtgcctc
    22381 ctcctttccg gcctcgcaaa tgcagaggac gtcaccggcc aggagctggc cttgcggggg
    22441 aaagttcgca actggcagag cccggaggaa caattagtcc tggacgtgca gtcggcccag
    22501 agcatagcgt cggccgcttc ggagagaaag taggggttca gtgcccagcg caaccgggga
    22561 agcaaaaatt cccgcgccgg taaggaaaaa tctggcagag tttactcccg gacaggtggg
    22621 agtcgtttct gaaattcacg gcgaggtgga gttgaccctg cggatgatgg aaatcggcct
    22681 ggtgccggga gtgcgcgtgc gctacctgcg cgccgcgcct ttgggcgatc cgattcaggt
    22741 cgaagtggag ggttttttgc tctccctgcg gaaaagcgaa gccgaagcgg tcatcctgca
    22801 ttccagcgag ctgccatgac agcgcaattg accgaaaaga acgttttcac ggttgccctg
    22861 gtcggcaatc cgaactgcgg gaagaccacc ctctttaacg ccctgaccgg cctgcggcag
    22921 aaggtggcga actaccccgg cgtgacggtg gagaagaaga ccggtctctg ggaaattgag
    22981 ggaaccactg tcgaagctct tgatcttccc ggcagttatt cactggttcc gcgctccccc
    23041 gatgaaaaaa tggttgcgga cgtattgtcg ggaaggcgtc cggatctgcc cgcgccggat
    23101 gccgtcatct gcgtcgtcga tgtaaccaat ctctcgcgtc acctttacct ggtcgggcag
    23161 gtgattgaat taggtcgccc gacggtagtg gccctgacga tggtggatga ggcggagcgg
    23221 ataggcctta ccgtggacac cgccaaactt tctgcccgtt tgggtgttcc ggtcgttgca
    23281 gtgatcgccg cgcgccgggt tgggctcgac caactggctc tggcggtcaa aaaaggagtc
    23341 ttcagtcaat ggcgatcacc ggagcggttc tggcggctgc tcccggcctt tgagaaccgc
    23401 gtaaaagcgt atgcgccgga agccgcagcg acggaattgg aaacggtggc ctgcgtcgcc
    23461 ggactccaca cccccgcctg caccgaaaag ttggaaaaag tcgcgaatca aattcgttcc
    23521 gaattagggg tgaacgaaga gaccgtccgc cgctacgtgg tggaagcccg ctaccgctgg
    23581 cagcgggagg tgacccgaga agtcgtcgag aaaacggggg accctcgggt cgattggacc
    23641 aaaagacttg ataagatttt tctgcactcc atccttggcc cgctcgtttt gctggttacg
    23701 ttaacgctgg tttttcagtc gattttcagt tgggcgcagg cgccgatgaa tgccatcggc
    23761 tacgtcaccg ggacgttttt gccgtcgctc acttataaaa tcctcgctcc cggcccgctt
    23821 tccagtttga ttaacgacgg catcttcgcc ggcgtcgggg cggtcgtcac tttcctgccg
    23881 caaatactcc tgctcttctt ctttctcacc tttatggaag actccggcta tctggcccgc
    23941 gccgcgtttc tcctcgaccg cttgatgtcc aaagttgggc tttccggacg cagtttcatt
    24001 ccgctcctgt cttcctttgc ctgcgccata cccggtatta tggccacccg gaccgttccg
    24061 caccaccgcg accggctggc gacgatttta gttgcgccgc tcatgacctg ttccgcgcgg
    24121 ctggtggtgt ataccctttt gattgccgcg ttcattccag ttgcacacgt ctgggggccg
    24181 ttcagcttgc aggggatgac gcttttgggc ctgtaccttc tgggaatatt cgccgccgcc
    24241 ggagtcgcgg cactattccg ccggacgatt ctgcgcgggc cgacgccgcc tttgcttttg
    24301 gaactgccgc cctaccgctg gccgaagccg cagacactct ttttgaccct ctgggaccgg
    24361 gcgaaaatct tccttgtttc agcggggacg atcatcttcg ccatttcggt tctcctctgg
    24421 gggctttcca gttacccgcg ctggacatcg attgagagtc acctcaccgc tgggcagaag
    24481 attttattct ctcagcccga cggtccacag aaacaggcgg cgctggaggc gctcaatcaa
    24541 aaattggcgg cggagcggtt agagcattct tttgccgggt atctgggaaa ggccattgcg
    24601 ccggtgctgc aaccactggg atttgactgg aaaatcggcg tgggcatcct cggctctttt
    24661 gcggcgcgcg aagttttcgt ttccacaatg ggcgtggtct atggcgtggg gagcgcggcg
    24721 gaaggccaaa cgttaacaga tcgtctgaaa gccgaccgct cacctgtaac cggcaccctg
    24781 gtctggacac ctctggtcgc aatcactctc ctcgttttct acgcttttgc gttgcaatgt
    24841 atgtccacca ttgccatcat ccgccgcgag acgaatacct ggcggtggcc ggcttttgcc
    24901 tttgcttata tgaccgttct ggcctatggc ggtgctttgg ccactcgttg gattgggcag
    24961 gcattctttg gaggataaag catgtttccg gtgaacttgc cgatggtggt tcaagatatt
    25021 tccgtcgcat cattctttgt tggcgcgggg cttttcctct tctggaaaaa gaggagcaaa
    25081 aagaacgctt cttgtccggc ttgtcaatcc gggtgcagcg gcccgaactt gcttgacacc
    25141 gcgaggacat ccaagaaaaa gcagactttt cgttaatcgg gagcagaact tacactcccg
    25201 accggctccg gggcgagcca gtctaccaaa tcctcggcga cggggcttga cagaaaaaat
    25261 ggagatggct attttattgg cgggaggggc agggacacgc caccggtgtc cgaaagggag
    25321 aggtgtgtcg gaacgaatgt gttattcaag tgattacatg gaagggagag cagccctgaa
    25381 gcggcttagt attttcggac tggccgctat tttctccctc gcctttcctt tttcatccgc
    25441 ctccagccaa accagtttgc tctaccggtt cgatggctcc gcccttgacg aactgggtta
    25501 ttccgtggcc gaggtcggag atgtagacgg cgacggcaaa gccgatttca tcatcggcgt
    25561 tccccgggcc gacccgaacg ggcttaccga tgcgggctcg gctttcgtct attccggcgc
    25621 aaccggagct ttgttgtatc gggtgaacgg cgccgccgat tttgactttt tcggtttttc
    25681 cgtagcggga gccggagaca ttgatggcga cagtcgagcc gattttatcg ccggcgctcc
    25741 ctttgcaagt ccggagggaa ttttcatcgc cggttccgca tttgtctact ccggcgccac
    25801 aggagttctg ctttaccaga agaacggcga accgggggac cggctgggac actcggtggc
    25861 gggcggggga gatatggatg gcgatgacag agccgatttt atagtcgggg catactttgc
    25921 cgatcccggc ggaatggtca atgccggttc ggcctatgtt tattcggggg cggacggcgg
    25981 tttgctttac cacaaagacg ggacggcggc tactgaccgg ttcggacaat ttgtggccgt
    26041 aacaggtgat ctgaatggcg acggcccggc tgattttata gtcggcgctc cccttaccga
    26101 tccgggcggt ctggcggatg ccggctcggc ttatgtctat tcgggagccg atggccacct
    26161 gctttaccgg ataaacggtg ccagcgccgg tgacggcctc ggcgttccgg tggcagggac
    26221 gggggatctg aatggcgacg gccaggcaga gttcctagta ggtgctactt ttgccgatcc
    26281 gaacgggctg gccgatgcgg gttccctctc ggtctattcc ggcaaggacg gttcgctcct
    26341 ctttcaagaa aatggggttt ccgccggcgg tcgtctgggc tttgcggtgt ccggggccgg
    26401 ggatttagac ggcgatggcg tacctgattt tatggccggt gctccgtttg ccgatccagg
    26461 cgggctggaa gacgccggct cggcattcgt cttttccggg gcgaacgggt tgctcctttt
    26521 tcagaaaaac ggcacgtccg gtgaccagct ggggtgggcg gtggccggga gcggggattt
    26581 gaatggcaac ggcagcgccg gttttatcgt cagtgcgcgc tacgccgacc cgggcgggct
    26641 tgtggatgcg ggctcggtat ttgtctatca gtgtctgccc cgcggggatg tgaacaatga
    26701 cggcgagacc gacgtcatgg acatcgtggg gttgatccag catgtggtgt tcgacgtttc
    26761 cttaaacagt ttgattgccg gagataccaa ctgcgacggg ttatctgaga ttgtggacat
    26821 cgtgcttttg atcgaatacc tggtgtacgg gacacccgcc ctgtgctgct tgtgaaaggt
    26881 cggagcaaag tagcgcggtt ttcgcgggcg gcgggacaga gatcatatcg aaatgtcaga
    26941 gagagcagaa caccggagaa gggagggtaa aatgaaacgc ttgagcattg tcagtgcggg
    27001 aattttgctc ctctgcgcag gggcaacgcg ggaggggata gccgggccgt ttgtcgattc
    27061 ggtcattgtg gtttccaggg tcttcagccc cgggagcacg ggcaccatag atattttgct
    27121 ccacaacgat tcgatcctct caggaattgg tctgccgctg gttatcaagt cggaaacggg
    27181 cggagcgttt ggcagaattg attccatcca gcgcttgccc tcgcttaatc cgtttgggat
    27241 gacctcagtc cttaaggtga atggcgtgac gccggactct gtatatatgc tctcggtcga
    27301 tagtggcgga ggcacggttt cgcccggctc aaggcgagcc gggtatcggc tctacgtgac
    27361 cagcaacaac aaccaaggaa ccttctcgct ggattccaac cggctatcat cagccaactc
    27421 cttgaagttt gcccacccgc cgacccagat acgtatacct gtcttcattc gcgggatgga
    27481 gataatcggc ggctcgctga gtctggcagc aacacaggtg actctgttca aaggaaagga
    27541 tagttgcgtg acggtgggag gaatgctctg cgatacaact tcccagacct tgaccctcga
    27601 cagcgtcagg gtccagcaca gcggcgatcc cgaccttttg tgtatcactg cgatcggggc
    27661 aacgaattta gtcgggaaga ccttgagtgc cttctcctgc gcgaccgatt cagggaacgg
    27721 atttttgaga gtctgtcagg cgcccacggc gtccatcggg gacacgggaa cctgcacgat
    27781 tacgttcttc ggcacgctac cgagtggcgc cggagcaccg gcacggatag tggcgttttc
    27841 ggcggcaatc tcgatggctt c
//
LOCUS       DBNGZD010000161         5686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1832287, whole genome shotgun sequence.
ACCESSION   DBNGZD010000161 DBNGZD010000000
VERSION     DBNGZD010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1832287"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..988
                     /locus_tag="AC16GM_04390"
     CDS             275..988
                     /locus_tag="AC16GM_04390"
                     /inference="COORDINATES: protein motif:HMM:NF053332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG domain-containing protein"
                     /protein_id="HIR2922950.1"
                     /translation="MSKLCKWLHEQLEQLPLMKFPFKLEELPENGIYFFYEKGEIWEH
                     GGNKLRIVRIGTHKDGNFRSRIEEHFLLDESRMNFSVTGLAPHWRSVFRKNIGRALLN
                     ERNDAYLQIWNVDFIPVKNREEFGPQRNIQKEKIIELKVTRVLREKFSFRFIIIDNQI
                     ERMGTKGLESALIGTVAQCGLCRPSSNWLGRFSPKKQIRESGLWLVQHLNADEIIESD
                     KADILDAIRRTKEWVTIGK"
     gene            1025..1648
                     /locus_tag="AC16GM_04395"
     CDS             1025..1648
                     /locus_tag="AC16GM_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922951.1"
                     /translation="MNARRFFGFSCTTLLGTMLVVACSSKEKQSATPEPGSSPAAAAH
                     GAGGNAINAGGLIWTAPARWEVGGERPMRAATYSVPAAKGDAEAGECAVYYFGQGQGG
                     DVQSNLQRWIGQFEQPDGKSSADAAKIAEKTVNTLHITTIELTGTYLASAGPMATSQV
                     NKPGFMLFGAIVEGPQGNVFFKFTGPKQTVSAAQKEFQSLIASVKKQ"
     gene            1723..2985
                     /locus_tag="AC16GM_04400"
     CDS             1723..2985
                     /locus_tag="AC16GM_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR2922952.1"
                     /translation="MNKTRTLLIYGLTFFLTAPAWALSKETFKQRRAKVLELLGDGVA
                     VLYGKSGGGDGDFRQDNNFYYLTGVEEPGAILLLAPKERTRREVLLLPTRNPEEERWT
                     GERPSLGKKLEDSLGFEYVQRTTGLGGIINSYLQHTKILSYISPPAAYSSPVPPDLSL
                     YRDVSSRVPETSIKNFSTLLPSLRVIKSAEEIAAIQKAIDITGAGFRAACQKVKPGMK
                     EEDVQDVIESTFKAEGARRLAFPSIVATGHNTTTLHYTRNDAVVKDGDLFLMDIGAEY
                     DHYAADVSRTIPVSGKFTPEQRKIYDIVLKAQKAAIAKVKPGAFIRDDVHNTARKVIE
                     EAGYGDYFIHSTSHFLGMDVHDVGDYSLPLQPGMVITVEPGIYLPDQDIGVRIEDDVL
                     VTENGCKVLTQGIPREVEEIERLLRERK"
     gene            3027..4811
                     /locus_tag="AC16GM_04405"
     CDS             3027..4811
                     /locus_tag="AC16GM_04405"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR2922953.1"
                     /translation="MGRGIKTLRPVIAGAVLMTLAVQSPPAGAQTAKSAALHKIFEDY
                     WEFVLQASPTFATYLGDHRYDDRLEDLSQEFYERRIAKAGEFLARLQAIPLDSLSSAD
                     SLNYDLFAREMQEQIEAAQFRPYLLPLSQQEGPHIGLVELTTYHPFFTPTDYENYIKR
                     LSLFPVQVEQILTNLREGLRTGIVPAKVTVEKIIPQIQAQVVAAPESSDLYQPVRKFS
                     DAFSPDDKVRLTDELKNAIASGVIPAYRKLLDFVQREYLPKCRTEAGIWALPDGDQRY
                     RFAVRHYTTTNLPPEEIFELGKKELARIQAEMKKIKEAAGFKGDLPAFIHYLRTDPKF
                     HYPTADSLVAGFKAILRRMDAKLPLLFGKLPQAHYDFREIERFRAAAAPDAYYYPPPE
                     DFSRPGYFYINTYKPESRPKYTMEALAYHEAVPGHHLQIAIQQELTDLPKFRRHGGYT
                     AFVEGWGLYSEQLPKEVGMYQDPYSDFGRLTMEAWRAVRLVVDPGLHFFKWTREQAIE
                     FFKANTALSEQNIVSEVERYIADPGQATAYKIGQLKIRELRAEAEKTLGPQFDVRAFH
                     DELLSDGALPLAILEKKMQRWVVRVNPN"
     gene            complement(5112..>5686)
                     /locus_tag="AC16GM_04410"
     CDS             complement(5112..>5686)
                     /locus_tag="AC16GM_04410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR2922954.1"
                     /translation="WWGLNDWIKENARKFAEQGYVALAVDLYRGRVTNSPDEAHELMR
                     GLPEDRAIRDLKAAAAYLQSRSDVKKNKIGAIGWCMGGGYALQLAINQPDLAACVMNY
                     GRIVTDSALIADIACPVLGIFGEEDRGIPVADVRQFEKACQRAGKTVNVHFYKAGHAF
                     MNPNNKSGYRPAATKDAWSMITTFFARSLK"
BASE COUNT         1338 a         1568 c         1586 g         1194 t
ORIGIN      
        1 tgttcgacct gcccgccgac gtcaaagacc cgcacctaat gctctcggag gcggcctggg
       61 aaacccactt tttgatcggg cacgagaata gcttcctgca caagaagacg acgtttcggc
      121 tgtcgattta aaggtttttt aagtaacttt ccagatggac acccactgaa tggagaactt
      181 gcttccaaaa ttagtttgta tttctccaaa agactcgtta atttcggtta agatttaaga
      241 taaccgaggc tcaaagagtt tatgaagggt tgaaatgtcc aaactctgta aatggttgca
      301 cgaacagttg gagcaattgc ccctcatgaa gttcccattc aaactggaag aactgcctga
      361 gaacgggatt tatttctttt atgaaaaggg agaaatctgg gaacatgggg gaaataaact
      421 acgaattgtt agaattggaa cgcataagga tggaaatttc agaagtcgga ttgaggaaca
      481 ttttctcttg gacgaatcaa ggatgaactt tagcgttact gggcttgctc ctcattggag
      541 aagtgttttt agaaagaata tcggaagagc tttgttaaat gaacgaaatg atgcgtatct
      601 gcaaatttgg aatgtagatt ttataccggt aaagaacaga gaggaatttg gaccgcagcg
      661 gaatattcaa aaagagaaaa tcattgaatt gaaggtcaca agagtactgc gagagaaatt
      721 ttcgttcagg ttcattataa tagataacca gattgaaagg atgggtacta aaggacttga
      781 aagtgctctt attggtacgg tagcacaatg tggattgtgc agaccttcaa gcaattggct
      841 gggccgtttt tccccaaaga aacaaattcg agaaagtggc ttgtggctgg tacagcatct
      901 taatgcggac gaaataatcg aatctgataa agccgatatt ttggatgcga taagaaggac
      961 aaaagagtgg gttactattg gcaaatgact gggcggttga tgttctcaat aaaggaggat
     1021 tccaatgaac gcacggcgct tcttcgggtt ttcctgcact actcttctcg gcacaatgct
     1081 ggttgttgcc tgttcctcca aagagaaaca atcggccacg ccggagcccg gttcatcccc
     1141 cgccgctgcg gcgcacggcg ccgggggaaa tgcgataaat gccggcggcc tcatctggac
     1201 ggcgcccgcg cgctgggagg tgggcgggga gcggcccatg cgggcggcga cgtattctgt
     1261 tcctgcggcg aaaggagatg ccgaagcggg tgagtgcgcg gtctactact tcgggcaggg
     1321 gcaggggggt gacgtgcaat ccaatctcca gcgctggatc ggtcagtttg agcagccgga
     1381 tggaaagtcc tcggcggatg cggccaagat cgccgagaaa acggtcaata ctttgcacat
     1441 taccaccatc gaactcaccg gaacctatct tgcctccgcc ggtccgatgg cgacttcgca
     1501 ggtaaacaag cccggcttca tgcttttcgg agccattgtc gaggggccgc agggaaatgt
     1561 cttcttcaaa ttcaccggtc ctaaacagac cgtttcggcg gcgcagaaag aatttcagtc
     1621 gctcatcgcg tcggtgaaaa agcagtaggt ttgactctca aaagggccac ctgcggcgcc
     1681 gggaattttg tcttccggag attcgggaca gaaggagttg agatgaataa aacgcggact
     1741 ttgctcatct acggcctcac gttttttctc actgctcccg cctgggcttt gtctaaagag
     1801 accttcaagc aaaggcgagc taaggtactc gaacttctgg gcgacggagt ggccgtcctt
     1861 tacggcaaga gcggcggggg agacggcgat tttcgtcaag acaacaattt ctactacctg
     1921 accggtgtgg aagagccagg agcaattctc ctactggccc cgaaggaacg aacccgcaga
     1981 gaagtgctct tgctcccgac ccgcaatccg gaagaagagc gctggacggg ggaaaggccg
     2041 tctctgggaa agaaattaga agattcgctg gggtttgagt acgtgcagcg gacgacgggc
     2101 ttaggcggga tcatcaactc ctacctccag cacacgaaga ttctctccta catctcaccc
     2161 ccggccgcgt acagttcgcc cgtgccgccc gacctctcgc tctaccgcga cgtctcctcc
     2221 cgcgtgccgg aaacgtcaat caaaaacttc tcaacgcttt tgccttcttt gcgggttatc
     2281 aaatcagccg aagaaattgc cgcgatccag aaggccatcg acatcaccgg cgcgggattc
     2341 cgagccgcct gccagaaggt caaacccgga atgaaagaag aggacgtgca ggacgttatc
     2401 gagtcaactt ttaaggccga aggggcaagg cggctggcgt tcccgtcgat tgtcgccacc
     2461 ggacataaca ccaccaccct gcactacacc cgcaacgatg cggtggtgaa agacggcgac
     2521 ctgttcttga tggacatcgg ggcggagtac gaccattacg cggcggacgt gtcgcgcacc
     2581 atcccggtgt cgggcaagtt cacgccggaa cagcggaaga tttatgacat cgtcctcaaa
     2641 gcccagaagg cggccattgc caaggtcaaa ccaggtgcct tcatccgcga cgacgtgcat
     2701 aacaccgccc gcaaggtgat cgaagaggcg ggctacggcg actacttcat ccacagcacc
     2761 agccactttt tgggaatgga cgtgcacgac gtgggcgact actcgctccc gctccaaccg
     2821 ggaatggtga tcaccgtgga gccggggatt tacctgccgg atcaggacat cggcgtgcgg
     2881 atcgaagatg acgttttggt gacggaaaat gggtgcaaag tgctcaccca aggcattccc
     2941 cgcgaggtag aagagattga acgacttctc cgtgaacgga agtaatcgat agttttgagg
     3001 aatttcaacc tgcgaagcga ggtattatgg gcagaggaat caagactctt cgtccggtca
     3061 tcgccggagc ggtgcttatg actttggcag ttcaatctcc gccagccgga gcgcagaccg
     3121 ccaaatcggc agcactccac aaaatcttcg aggactactg ggaatttgtc ctgcaagcgt
     3181 ctccgacgtt tgccacctac ttgggtgatc accgctacga cgaccggctg gaagacctct
     3241 cgcaggaatt ctacgagcgg cggatcgcca aggccggaga gttcctggcc cggctgcagg
     3301 caattccgct cgattcgctg tcatccgccg acagtctcaa ctacgacctc tttgcccgtg
     3361 aaatgcagga gcagatcgaa gcggcccaat ttcggcccta cctcttgccg ctatcccagc
     3421 aggagggacc gcacatcggg ctggtggaac tcaccaccta ccaccccttc ttcacaccca
     3481 ccgactacga gaactacatc aaacggctct ccctattccc ggtgcaggtc gagcagattt
     3541 taactaatct gcgagaaggt ctgcgcaccg gcatcgttcc ggccaaggtg acggtggaga
     3601 aaattattcc ccagattcaa gcgcaggtgg tggccgcacc ggaatcgagc gacctctacc
     3661 agccggtcag gaaattttcg gatgcttttt cgcccgacga taaagtccgg ctcaccgacg
     3721 agctgaagaa cgccatcgcc agcggggtca ttcccgccta ccggaaactc ctcgatttcg
     3781 tgcagcgcga gtacctcccc aaatgccgca ccgaggcggg catctgggcc ctgccggatg
     3841 gcgaccagcg ctaccgcttc gccgtccggc attacacgac caccaatctt ccgccggaag
     3901 agatttttga attgggcaaa aaagaactgg cgcgcattca ggcggaaatg aaaaagatca
     3961 aggaggcagc gggattcaag ggcgatttac cggcgttcat tcactacctg cggacagatc
     4021 cgaagttcca ctacccgacc gccgactcgc tcgtggccgg gttcaaagcg attctgcggc
     4081 ggatggatgc caaactgccc ttgcttttcg gaaaactgcc ccaggcgcac tacgacttcc
     4141 gggagatcga gcgtttccgc gccgcggcag caccggacgc ctactactat ccgccgccgg
     4201 aagatttctc ccggcccggc tacttttaca tcaacaccta caaaccggag agccggccca
     4261 agtataccat ggaggcctta gcctatcacg aagcggttcc cggccaccac ctgcagatcg
     4321 ccatccagca ggaattaacc gacctgccga aattccgccg ccacggcggc tacaccgcct
     4381 tcgtcgaggg ctgggggctc tactcggagc agctacccaa agaagtgggg atgtaccaag
     4441 atccctattc cgatttcggc cggctgacta tggaggcctg gcgggcggtg cggttagtcg
     4501 tcgatccggg cctgcacttt ttcaagtgga cgcgcgaaca ggcgattgag tttttcaaag
     4561 ccaataccgc actctccgaa cagaacatcg tctccgaggt ggagcgctac atcgccgacc
     4621 ccggacaggc cacggcctac aagatcggcc agttgaaaat ccgggaactg cgggcggagg
     4681 cggagaagac tttaggcccg cagtttgacg tccgcgcctt ccacgacgaa ctcttgagcg
     4741 acggcgcgct gcctctggct attctggaaa agaagatgca gcgctgggta gtgcgggtga
     4801 atccaaacta agccatttga ctccgccgag gatgaaatcc tcggctacag ggcccaaggg
     4861 tcattcggat tttcgtagcc gaggttttta acctctgcgg ccggtagacc gccgcctttt
     4921 ataattcaac gcccggacgg gcgccgagaa tgtaattctc gactacggaa ctggtgggaa
     4981 ttccgatttg atatttgtag ccgagagttt tagcctcggc gatccgcagg atcgctgatt
     5041 taaatattaa agtcgggctc agggacgagc ccgactaccc acaatcgctt ctcgtattcg
     5101 accgatcgtg gctacttgag gctgcgcgca aagaaggtcg taatcatgct ccaggcgtct
     5161 ttggtcgccg ccgggcggta gcccgacttg ttgttcggat tcatgaaggc gtggccagcc
     5221 ttgtagaaat gcacgttgac cgtcttgccc gctctctggc aggccttttc gaactggcgc
     5281 acgtcggcca ccggtatgcc acggtcttcc tccccgaaaa tccccaaaac cgggcaggcg
     5341 atatcggcga tgagcgccga atcggtgacg atgcggccgt agttcatcac gcaggcggcc
     5401 aaatcgggct ggttaatcgc cagttgcagt gcatacccgc cgcccatgca ccagccgatg
     5461 gcgccgattt tattcttctt cacgtcactc cggctctgca agtaggccgc cgccgctttc
     5521 aggtcgcgga tggcgcggtc ctccgggagg ccgcgcatca gttcatgcgc ttcgtcgggc
     5581 gagttggtaa ctctgccccg gtagagatca acagccaggg ccacgtagcc ctgctcggcg
     5641 aattttcgcg cattctcctt gatccagtcg ttcaagcccc accatt
//
LOCUS       DBNGZD010000162         4385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_582615, whole genome shotgun sequence.
ACCESSION   DBNGZD010000162 DBNGZD010000000
VERSION     DBNGZD010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_582615"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="AC16GM_04415"
     CDS             complement(<1..755)
                     /locus_tag="AC16GM_04415"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR2922955.1"
                     /translation="MTERLIEVAFPLSVRHQFTYRVPPELRERVVPGMRVLAPLGQRQ
                     KIGFVVGEATHRPKLIKEITDLLDPEPIVVPDLLRLTRWMADYYFSSWGECLQAVLPA
                     GIQGQVAETIRWNPEVKAESILAGNHWSAAEKNFLTLLSQEHSLKISVLKRTFGTREL
                     YARLIDFERRGFLVLETSIQRHQSEPLTHQYVRLTIGLAKEQQTTFAQYLAALPEKQK
                     KKRGTLQFLADQAKPVASQLLVERFGVGRAQLRR"
     gene            complement(868..2022)
                     /locus_tag="AC16GM_04420"
     CDS             complement(868..2022)
                     /locus_tag="AC16GM_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922956.1"
                     /translation="MKTAQVAMLLGAVACSLAFTPATLKADCKPADYYTRSAKVNYIQ
                     IRNPDYKKALALLNEGRACYPKNAEIYFWLGNLYGELDSIKLMSAFYDTSVSLSDKYK
                     KEITNTRVSKWGDLFQTGLNALKADTVQSDSLKLATRLRGKERLRKATVVDPTRYEAF
                     VNLGVAYLKLNQPESALVCYETAHKLNPKDVAIISSLAGNYFTQKRFSEAVKLYQEIV
                     AIDPQNKDALLAMGAAYDQLGETEKVKETYLRVLQIDPKDKNVLFNMGLFALRAVADT
                     TKHFQAILEKNPNDTKAQAEQQRIQKIYYASAESLFVRASQADTTDKYAWHYAGVCAR
                     IMDHVDEALTYQLKAVAVDDNFKDAYQELAVLYTLKNMPDKAKEAEAKSK"
     gene            complement(2051..3448)
                     /locus_tag="AC16GM_04425"
     CDS             complement(2051..3448)
                     /locus_tag="AC16GM_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024985.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HIR2922957.1"
                     /translation="MSLLNRENGRRRMVIRQSAFRFVVLALFTLTADHTSAQKEVSLT
                     IYNQDLGLVKEIRTLDFARGRNRIQFTDVASAIDPTSVRFRALEHPESINLLEQNYQF
                     DLVNSEKILSRYVDKSIQAIAKDGKVFEGTLLSVSPGSLVLQEPSGGLRIVSRDNILD
                     IAFPALPEGLFTRPTLLWLIDAETAGKRKAEVSYLTSAINWHAEYVATVDATDTNLEL
                     AGWVSIDNRSGAGYADAKVKLIAGEIHRAEAPRPRYAEGLAMEQMAKAPAFAEEAFFE
                     YHLYTLQRPATVANNEIKQISLFEPAQTPVKKIFTFEGQRRAKVNVTLEFVNAQARGL
                     GMPLPAGKIRVMKKSADGGMEFVGEDMIDHTPKDEKVRIEVGSAFDLVGERKVKDRRQ
                     ISDRVWEEDYEISLRNRKAENVEIVVTEHLYGYWEIRNASSPYTKKDANTVEFPVKVP
                     AGAEVVLTYTARFRS"
     gene            complement(3516..>4385)
                     /locus_tag="AC16GM_04430"
     CDS             complement(3516..>4385)
                     /locus_tag="AC16GM_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR2922958.1"
                     /translation="EIKDPAMLSLPDRWIFSRLHQTIQTMTAQLDDFRYNAAAKTLYD
                     FAWGDFCDWYLELSKVLLKNAASGAKRKTQAVLFTTLNDLLLLLHPFLPFLSEELWHK
                     LNGIPENDSFRSILQEHWPQANAKLINAGLEKEVAWLQSLITAVRALRGELDIPPVKK
                     SRLVVSVDDLAQKESLEFQLDFVRELARAEAVEVGQHLRRPHQSAVAVLSGAEVYLPL
                     EGLVDIAAEKSRLSRAKEKYEHLLSAVERKLATPEFAQKAPPEVVQKEETKRADFAKA
                     VAKLQALVEQLEK"
BASE COUNT          843 a         1203 c         1298 g         1041 t
ORIGIN      
        1 cggcgcaact gggcgcgacc caccccgaag cgttcaacca gcaattggga agcgaccggt
       61 ttagcttggt cggctaaaaa ctgcaaagtg ccccgctttt ttttctgttt ctccggaagt
      121 gctgccagat attgggcaaa ggtcgtctgc tgttccttgg cgagaccaat agtcagacgc
      181 acgtactggt gggtcaaagg ctcgctctgg tggcgctgga tggaggtttc cagcaccaga
      241 aagccgcgcc gctcgaaatc gattaaacgg gcgtagagtt cccgggtgcc gaaagttctc
      301 ttgagtacgg atattttgag cgaatgctct tgggagagaa gggtaaggaa gtttttctct
      361 gctgccgacc agtggttgcc ggccaggatc gactccgctt tgacttctgg attccagcgg
      421 atggtttcgg ccacctggcc ctgaatcccc gctggcagga ccgcttgcaa acattcgccc
      481 caggaagaga agtagtagtc ggccatccag cgggtgaggc gcaaaaggtc ggggacgacg
      541 atcggctccg gatcgagcag gtcagtgatt tctttgatca gtttcggccg gtgggtggct
      601 tccccaacga caaaaccgat tttttgccgc tggccgaggg gagccagaac gcgcatgccg
      661 ggaacgaccc gctccctcag ttccggcggg acgcggtagg tgaactggtg ccgcaccgag
      721 aggggaaaag cgacttcgat taagcgttcg gtcatgagtc agtaatatgt agagcaaaag
      781 acccaaaaag aaagccacgc tcagaaaagc gtggctacag gcgaaataaa ttcttcaccc
      841 tcgaaacacc gccagcggct actgaggtta cttcgatttc gcttcggcct ctttggcttt
      901 gtccggcata ttcttcagcg tgtagaggac cgccagttcc tggtaggcgt ctttgaaatt
      961 gtcatcgacg gcgacggcct tgagctggta cgtaagggct tcatcgacgt gatccataat
     1021 gcgggcgcag acgccggcgt agtgccaggc gtacttgtcc gtcgtatcgg cctgggaggc
     1081 ccggacaaag agcgattcgg ccgaggcgta gtagattttc tggatccgct gctgttcggc
     1141 ttgggctttc gtatcgttgg gatttttctc caggattgcc tggaagtgct tggtggtgtc
     1201 ggctacggcg cggagggcaa agagccccat gttgaacagc acgttcttgt ctttcgggtc
     1261 gatctggagc acccgcaggt aggtctcctt cactttttcg gtttcgccca actggtcgta
     1321 ggccgcgccc atggccaaaa gcgcatcttt gttctgggga tcgatggcaa caatctcctg
     1381 atacagtttc accgcctccg agaagcgctt ctgggtgaag tagttccccg ccagactgga
     1441 gatgatggcg acgtccttgg ggttcagttt gtgggccgtc tcgtaacaaa ccaaggccga
     1501 ctcgggctga ttgagcttca gatatgcgac gcctaagttg acgaaggcct catagcgggt
     1561 gggatcgaca acggtggctt tccgcaagcg ctccttgccc ctcaggcggg tagcgagttt
     1621 gagactgtcg ctctgtaccg tgtcggcctt gagggcgttc aatcccgtct ggaacagatc
     1681 gccccacttg ctgacccggg tattggtaat ttccttctta tacttgtccg aaagggagac
     1741 ggaagtgtcg tagaaggcgc tcatcaactt gatcgagtcc agttcaccgt agaggttgcc
     1801 cagccagaag tagatttcgg cgtttttggg atagcaggcg cggccctcat tcaaaagcgc
     1861 cagcgccttc ttgtagtccg ggtttctgat ctggatgtag ttcaccttgg ccgagcgggt
     1921 gtaatagtcg gcgggcttgc agtcggcctt aagtgtggcg ggtgtgaaag ccaaagaaca
     1981 ggctactgcc cctaacagca tggcaacttg tgcggttttc attgccctcc ctttcgttac
     2041 tctatccatt tcaactccgg aagcgggcgg tgtaggtaag caccacctcg gcaccggctg
     2101 gcacttttac ggggaattcg accgtgttgg cgtctttttt cgtgtaggga gaactggcat
     2161 tcctgatctc ccagtagccg tacaggtgct cggtcactac tatttctacg ttttccgcct
     2221 tgcggttgcg cagggaaatc tcgtagtcct cctcccagac ccggtcggaa atctgccgcc
     2281 ggtctttcac cttccgctcc cccaccaggt cgaatgcgga gccgacttca atccggactt
     2341 tctcgtcttt gggggtgtgg tcgatcatat cttcgcccac gaattccatt cccccgtcgg
     2401 cggacttttt catcacccgg attttgcccg ccggcagggg cattcccagc cctctggcct
     2461 gggcgttgac gaattccagc gtcacattta ctttcgcgcg cctttgaccc tcaaaggtga
     2521 agattttctt gacaggggtc tgggccggct caaacaggga gatctgtttt atctcattat
     2581 tggcgacggt tgcaggccgc tggagggtat acaggtggta ttcaaagaag gcctcttcgg
     2641 caaaggccgg ggccttagcc atttgctcca ttgccagccc ttcggcatag cgggggcgcg
     2701 gcgcctcggc ccggtgaatt tcaccggcga tcagtttgac tttggcgtcg gcgtaaccgg
     2761 ccccggaacg attgtcgatc gacacccagc cggccaattc gaggttggtg tcggtggcat
     2821 ccaccgtggc cacgtactcg gcgtgccagt tgatggcgga ggtcaggtag ctcacttcgg
     2881 ccttgcgctt ccctgctgtt tccgcgtcga tcagccagag cagcgtcggc cgggtgaaga
     2941 gcccttccgg cagggcgggg aaggcaatgt ccaggatgtt gtcccggctc actatgcgca
     3001 gaccgccgct gggttcctgc aggacaagac tccccggcga aaccgaaagc aaagttccct
     3061 cgaagacctt gccatctttg gcgatggctt gaattgactt atcaacatag cgggagagga
     3121 ttttttcgga attgaccagg tcgaactggt aattttgttc caacaggttg atgctttccg
     3181 gatgctccag cgcccgaaag cggaccgagg tcgggtcgat ggcggaggcc acgtcggtga
     3241 actggatgcg attccttccg cgggcaaaat ccagcgtcct gatttctttc acgagtccta
     3301 agtcctgatt gtagatcgtg agactcactt ctttttgcgc cgaagtatgg tcggcggtca
     3361 gggtgaagag agccagcacc acgaatcgaa acgcactttg ccggatcacc attctcctgc
     3421 ggccgttttc ccggttcaaa agcgacatgc gctcctcctt ttctggtcaa ccgggatacc
     3481 ggcggagccg ggaaatattc ccgaacatgt cgacatcact tttccaactg ttccacaagg
     3541 gcctggagtt tggccaccgc tttggcgaag tcagcccgtt tggtctcttc tttttgcact
     3601 acctccggcg gggccttctg tgcgaattcg ggcgttgcaa gttttcgttc caccgccgat
     3661 agcaggtgtt cgtacttctc ctttgcccgg gagaggcggc ttttttcggc ggcgatgtcc
     3721 accagtcctt ccagaggcag gtaaacctcc gctccggaga gaaccgccac cgccgattga
     3781 tgcgggcgtc tcaagtgttg ccccacctct accgcttcgg cacgagccag ttcgcgcacg
     3841 aaatcgagtt ggaattcgag ggattccttt tgtgccagat cgtccacact gaccaccagc
     3901 cggctcttct taacgggcgg gatgtccaat tcgccgcgga gggcgcgcac agcggtgatg
     3961 agcgactgga gccaggcgac ctctttttcc agtccagcat tgatgagttt ggcgttcgcc
     4021 tgcggccaat gctcctggag aattgagcgg aaggagtcgt tctccgggat gccgttcagt
     4081 ttgtgccaca attcctcgct cagaaatggc agaaagggat ggagcaaaag caagaggtcg
     4141 ttcagcgtgg taaagagcac cgcctgcgtc tttcgtttgg cgccggaggc ggcatttttc
     4201 aagagcactt tggacagttc caagtaccag tcgcagaagt cgccccaggc aaaatcgtag
     4261 agggtcttgg cggcggcgtt atagcggaag tcatccagct gggcggtcat cgtctggatg
     4321 gtttgatgca agcggctaaa aatccagcgg tctgggagac tcaacatagc cggatccttt
     4381 atctc
//
LOCUS       DBNGZD010000163         2093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_859839, whole genome shotgun sequence.
ACCESSION   DBNGZD010000163 DBNGZD010000000
VERSION     DBNGZD010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_859839"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16GM_04435"
     CDS             <1..759
                     /locus_tag="AC16GM_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922959.1"
                     /translation="GLVLVCNTGFNPVDFSYGQGTVAVFDPAVDSVVKVIPVGSNPQA
                     IAQAPSGVLHVVCTGNYVNISDKIYEIDPATLTVVDSILIGGQPGAIGIGPNGIAYVA
                     AGGFVGSGIVFSYDTFTGTVLHGRGNPIATGTGASAVAVDNLNQALVCDFQAATVTQL
                     DSLGNPLATYALGDGPLSVAIYDTTRLGDLDGDGLVDLADIVTAVQHVVFDRRPLVIR
                     LADVNADCVVDLLDIIVLVRHIVFGTPQLLPGCA"
     gene            complement(795..1916)
                     /locus_tag="AC16GM_04440"
     CDS             complement(795..1916)
                     /locus_tag="AC16GM_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR2922960.1"
                     /translation="MKKQSWFWAWSGAGVLVVSGVLLSSCVEKVPPPLGAAPILSGNF
                     VTLKPENLDSLPSSQVYELWLLRSTPHGTEQKSLGKFRWDKFLYAARHPDGSRITENK
                     FDAGEDVFAWAEIAITIEQANDPSPAPSASVFFLGTINDTLPDLRLTYPINLTKEEFK
                     GDFFLASPTDSAGFEFLYTDFTTPPLADRQRRESQGIWFGDLALAKEAKRDTAQFVGV
                     TPGLVLPKLSAGWQYEGWVLVGGKYLSTGKFLRADSADFTNPYLGPKATGKQTFTSGD
                     SLRFTTDFKIPGEDFILNPPSGFVFPLSMIRTPASGDSVFVTIEPSPDPSPATPFRSL
                     TLYGAQLPTALSSSFIHQNLGLANISRRAPAVAVSISDK"
BASE COUNT          420 a          566 c          664 g          443 t
ORIGIN      
        1 ggtctggtct tggtctgcaa caccgggttc aatccggtcg acttttccta cggccagggg
       61 acggtggcgg tgttcgatcc ggcggtcgat tcggtggtca aggtgattcc ggtcggcagc
      121 aatccgcagg caatcgctca agctccttcg ggagtgctcc acgtcgtttg cacgggaaac
      181 tacgtgaaca tatccgacaa aatttatgag attgatccag cgacgctgac tgttgtggat
      241 agcatcctca tcggcgggca gcccggggcg atcggtattg gaccgaacgg gattgcgtac
      301 gtcgcggccg gcggttttgt cggcagcggt atcgtcttta gttacgatac cttcaccggc
      361 actgtgctcc acggacgcgg gaatccgatc gccacgggaa ccggcgccag tgcggtggcg
      421 gtggacaacc tgaaccaggc gctggtgtgc gactttcagg cggcgacggt tacccagttg
      481 gattctctag gcaacccgct ggcgacttac gccttgggtg acgggccgct ttcggtggcg
      541 atctacgata cgacgcggtt gggtgatctg gacggcgatg ggctggtgga tcttgccgat
      601 atcgttaccg ccgtccagca cgtggtgttt gatcgccggc ctctggttat tcgtctggcg
      661 gacgtgaatg ccgactgcgt ggttgatctg ctggacatta ttgtgttggt ccggcacata
      721 gtcttcggga cgccccagct tttgcccggg tgcgcctgac ctggcagagc gtccgtccgt
      781 gtggagtgcg gcgattactt gtcgctgata ctcactgcga ccgccggcgc gcggcgggaa
      841 atattcgcca atccaagatt ttgatggatg aaggaactgc tgagagcggt cgggagttgc
      901 gcgccataaa gcgtcaaaga gcggaagggt gtggccggac tgggatctgg agacggctcg
      961 atggtgacaa aaaccgaatc cccggaggca ggggtgcgaa tcatggagag ggggaacacg
     1021 aaccccgacg gagggttgag gatgaaatct tcaccgggaa ttttgaaatc ggttgtgaac
     1081 ctcagcgaat ccccactggt gaaggtctgc tttcccgtgg ctttcggccc gaggtacgga
     1141 ttcgtgaagt cggctgagtc agcacggaga aatttccccg tggacaggta ttttccgccg
     1201 acgagcaccc agccttcgta ctgccaaccc gcggagagtt tcggcagcac taaaccgggt
     1261 gtgacgccga cgaattgcgc cgtgtcccgt ttggcttcct tggcgagggc taagtcacca
     1321 aaccagatcc cctgtgattc acggcgctga cggtcagcca ggggcggggt ggtaaaatcg
     1381 gtatagagga attcaaaacc ggctgagtcg gtggggctgg ccaagaagaa atcgcccttg
     1441 aattcttcct tcgtgagatt gatggggtag gtcaggcgca ggtcgggcag ggtgtcgttg
     1501 atcgtcccca aaaagaaaac gctggccgag ggcgccggac tggggtcatt ggcctgctcg
     1561 atggtgatgg cgatctctgc ccaggcaaaa acgtcctcgc cggcatcaaa cttattttcg
     1621 gtaatccgtg agccgtccgg atgccgggca gcgtagagga atttgtccca gcggaatttg
     1681 ccgagacttt tttgctcggt cccgtgcggt gtggaacgga gaagccagag ctcatagacc
     1741 tggctggaag gtagcgaatc taaattctcc ggcttcaacg tgacgaagtt gccggagaga
     1801 atcggcgccg cccccaaggg cggcggaact ttctcgacgc aggatgaaag taataccccg
     1861 ctcacaacga gcacccctgc accgctccac gcccaaaacc aactctgctt tttcatagtc
     1921 actcccgcat cgctcctgaa gtacccatct accgggaact acaatacagc ccctctcgtc
     1981 aaatgtcaag cggtgattcc ctgcctcacg ggccggtgaa ctgccaggag gccagcatcc
     2041 cggctacctg ttccagattg atccggaaga actggcggtc gcagatgaaa taa
//
LOCUS       DBNGZD010000164         3094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1624567, whole genome shotgun sequence.
ACCESSION   DBNGZD010000164 DBNGZD010000000
VERSION     DBNGZD010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1624567"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="AC16GM_04445"
     CDS             complement(<1..489)
                     /locus_tag="AC16GM_04445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2922961.1"
                     /translation="MPPKSPLSFGSPAVGARQAVFSASRPVLLPSEKTNYFDWYWETR
                     DLDTADLRSIERARIVLGLLGRPYGKLLDAGCGRGLAAALFQQAGFTVTGWDISPEAV
                     AVARAFNVSAQVVDIEEDRLSGKFDVIVLSEVLQQVADPLRVVLRLKKVAHSGCRWVI
                     SLP"
     gene            complement(479..1660)
                     /locus_tag="AC16GM_04450"
     CDS             complement(479..1660)
                     /locus_tag="AC16GM_04450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2922962.1"
                     /translation="MRLCLITNRFPTHPDDTASPFVGDFYRGLRERGVEVFVFTPEYP
                     TVRLSPDPRVCRFTWLGSNVPIGSLDYRKPANLLRLASFVLSGRNQLLEFLQVKEINH
                     CLALWALPSGYLAWYARQQLGIPYSVWCLGSDIYVWAQKPLLHGLTVSVLSEATHLYA
                     DGFDLAHRATGLAGKACSFLPSARRLPPACLSGNNFGTATEFLYVGRLDPDKGLAELL
                     LAFEIVSRRYDCRLILVGWGSLEEEIRRWIAQRHLEHKITLVGRQGAAELANWYARAD
                     CVVIPSRSDSIPLVVSEAAQFKKPVIATRVGDLGRVVRRYRLGWVVPPANVETLAQAL
                     EEFIHKGTAVRPDFKGLLQLLSIEQAVNQVIKDFGIATVRQPQPLPTGSSEKRSYLYA
                     S"
     gene            complement(1617..2690)
                     /locus_tag="AC16GM_04455"
     CDS             complement(1617..2690)
                     /locus_tag="AC16GM_04455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2922963.1"
                     /translation="MSRSQRMEAIAQPEVSVVVPMHNESANLPVTLARIAQALRLSGK
                     SWEIIAVNDGSTDDTERKIQAFAREELNVRLVSYQPNHGRGYALRQGFAAARGEIICT
                     TDADLSYGEEYLPAMVKVLQEHPEVDVVIGSPYMPGGKVEKVPKFREMISRAANKILG
                     FALPGNLATVTGILRAYRRPSLEALGLESDGKEIHLEILSKAVAMGFGVAEMPATLWG
                     RQKGKSKFKFRATSISHLLFSFFEKPMLLFGLVGLLLFSLAVLGGGYVIYLWQTGQLN
                     PNRPLMTLIVILSLTGLQVLLFGFLGTQIVHLRREIYRLQRTTRVMAQSLGRQPAEPS
                     VPGTDTKKIYAALPDYQSLSHSS"
BASE COUNT          661 a          937 c          931 g          565 t
ORIGIN      
        1 gggcagagag atgacccagc ggcagccgga atgggccact tttttcaggc gcaggaccac
       61 ccgcagcgga tcggccacct gctggagcac ttcggaaaga acgatcacgt caaatttgcc
      121 ggagagccgg tcctcttcta tgtcgaccac ctgggcgctg acgttaaacg ctcgggccac
      181 cgctaccgct tccggcgaaa tgtcccagcc ggtcaccgta aacccggcct gctggaagag
      241 cgccgccgcc agaccccgtc cgcagccggc atccaagagc tttccgtatg gccggccgag
      301 aagccccaga acgatccggg cccgttcaat actccgcagg tcggcagtat ccagatcgcg
      361 cgtctcccag taccagtcga agtagttggt cttttcactg ggcaaaagca ccggccggga
      421 ggccgaaaaa acagcctgcc gggcgccgac tgcgggcgaa ccgaaggaga gcggcgattt
      481 aggaggcata caggtaactc cgcttttcgc tcgatccggt tggcaacggc tgcggctggc
      541 ggacggtggc gatgccgaaa tccttgatga cctgattcac cgcctgctcg atgctcaaaa
      601 gctgcaaaag ccctttgaaa tcgggccgca ccgccgtccc cttgtggatg aactcttcta
      661 aagcctgggc cagcgtttcc acgttggccg gcggcaccac ccagcccagt cggtaccggc
      721 ggaccacccg gcccagatcg cccacccggg tggcgatcac cggttttttg aactgggccg
      781 cttcggacac aaccaaagga atgctgtcgg agcgggaagg gatcaccacg cagtcggcgc
      841 gggcatacca gttggccaat tctgcggccc cctgccggcc aaccaaggtt atcttatgct
      901 ccaggtgacg ctgggcaatc caacggcgaa tctcttcttc gagacttccc cagccgacta
      961 agatcaggcg acagtcgtac ctccggctaa cgatctcaaa agcgagcaaa agttccgcca
     1021 gccctttgtc cggatccaga cgccccacgt agaggaattc ggtggctgta ccgaagttgt
     1081 tcccggacag acacgcgggg ggtaagcgcc gggcgctggg caggaaagag caggccttcc
     1141 cggccaagcc ggtggcccga tgggccagat caaaaccgtc ggcgtacaga tgcgtcgcct
     1201 ccgagaggac gctgaccgtc aggccgtgca gcagaggttt ctgcgcccag acgtaaatgt
     1261 cggaacccag acaccagacg gagtacggta tgcccaactg ctggcgagcg taccaggcca
     1321 ggtagccgga aggcaaggcc cacaaggcca agcagtggtt gatctctttg acctgcaaga
     1381 actccagcaa ctggttgcgt ccggagagaa caaaactggc cagccggagg agattggcgg
     1441 gcttgcggta gtccagtgag ccgatcggca cattggaacc cagccaggtg aaacggcaga
     1501 cccggggatc gggagagagg cgcacggtag ggtactcggg tgtgaataca aatacttcca
     1561 ctccccgctc gcgcagaccg cggtagaaat cgccgacgaa aggcgaggcg gtgtcgtcag
     1621 gatgagtggg aaagcgattg gtaatcaggc agagccgcat agattttctt ggtatcagtc
     1681 ccggggacgg atggttccgc cggctggcgg cccagagact gggccattac ccgggtggtg
     1741 cgctgcaacc ggtagatttc ccggcgcagg tggacgattt gcgttccgag aaaaccgaac
     1801 aagagcacct gtaatccggt cagcgaaaga atcacgatca aggtcatgag cggccgattg
     1861 ggattcaact gcccggtctg ccagaggtag atgacgtagc cgccgccgag caccgcaagg
     1921 gaaaagagga gcaaaccgac cagcccgaag agcagcatcg gtttctcgaa gaacgaaaac
     1981 aacaggtgcg atatggaagt ggcgcggaat ttgaatttcg acttgccctt ctgacgcccc
     2041 cagagggtgg ccggcatctc cgccacgccg aaccccatgg cgacagcttt cgagagaatc
     2101 tccaggtgaa tttcttttcc gtccgactcc agaccgagcg cttccagaga gggacggcgg
     2161 taggcgcgca ggatgccggt gacggtagcc agattccccg gcagggcaaa acctaaaatc
     2221 ttatttgcgg cgcggctgat catttccctg aatttgggga ctttttccac ttttcccccc
     2281 ggcatatagg gtgaaccgat gacgacatcg acttccgggt gctcctgcaa taccttgacc
     2341 atagccggca ggtactcctc gccgtaactc aaatcggcgt cagtggtgca gatgatttcc
     2401 ccccgcgcgg ccgcaaatcc ctgcctcagc gcataccccc ggccgtggtt gggctggtaa
     2461 ctcaccagac ggacgtttaa ctcttcccgg gcgaaggctt ggatcttccg ttcggtatcg
     2521 tcggtggagc catcgttgac ggcaataatt tcccacgact taccggaaag acgcagggcc
     2581 tgggcaatcc gcgccagggt gaccggcagg ttggccgact cgttgtgcat cggcacaacg
     2641 accgaaacct ccggctgggc tattgcctcc atcctctgtg acctactcat cctctctctg
     2701 gcatcggcgg ctggcgggtg ttccttgagt gatgcggctt tctgctcggc gatctacttg
     2761 acccctcccc gcctgaggcg gatcttcgac ctcaccctcc cctcaagggg agggaaggat
     2821 tcttcccttt gtgaaggaat gacggagtta catttaagcg caggaggcgt tacccaaaca
     2881 aaaaccccgc cctgcgcttg atgcaggacg gggtttgtac gaactgcgga aaagatcatg
     2941 tctccgcgtc gttgatggta cagctcagcg tgccggtttc gtcaatctgc caggtgtcga
     3001 tcgtcgcatc gtcgtccagg ttcgcggtcg cgatagcgaa gaaggagtta acaatccccg
     3061 caccacccgc gttgatcacg tagctgtacc gtgc
//
LOCUS       DBNGZD010000165         4141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_513707, whole genome shotgun sequence.
ACCESSION   DBNGZD010000165 DBNGZD010000000
VERSION     DBNGZD010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_513707"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1104)
                     /locus_tag="AC16GM_04460"
     CDS             complement(169..1104)
                     /locus_tag="AC16GM_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR2922964.1"
                     /translation="MAGYILILDDCFSWLARREPHSIHAVVTDPPYGLKEYSPSEQKK
                     LRRGRGGVWRLPPSFDGCQRSPLPRFTILNRADQNAIRQFFTEWAQLIFRVLTPGGHV
                     FIATNSLLSHLVYQPMIEAGFEKRGEIIRLVTTLRGGDRPKNAHVEFRDVTVMPRARW
                     EPWGLFRKPCEGRVQDNLRKWSTGGLRRVSAEIPFSDVIPSGPTRPEERGLANHPSLK
                     PQAFMRQIVHASLPLGEGIVLDPFMGAGSTIAAAEAVGYASIGIEKDPHYFRMARRAI
                     PRLAALSLNGNMSGRFVNFARISQRVTRTVESPKK"
     gene            complement(1344..2180)
                     /gene="larE"
                     /locus_tag="AC16GM_04465"
     CDS             complement(1344..2180)
                     /gene="larE"
                     /locus_tag="AC16GM_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR2922965.1"
                     /translation="MSAEKLQRLAEELRTLGSVLVAYSGGVDSAFLLWAACRFLGNER
                     VLAVTANSASYPPEELESARSFARLLGLAERHLVVETDEVASENYAQNSPARCYFCKE
                     ELYTKLTPVAAQYDIPFIVDGFNASDAGDFRPGKKPADIFGNVKSPLLAAGLTKPEIR
                     EIAKAHDLPFWNKPAMACLSSRVPYGQRVTPEKLAQIAQAERFLRSLGFRQFRVLHHE
                     QVARIEVEPEELSRFLDEGIRTAIADRLREIGFLYVTLDLAGYRSGSLNEALALERKG
                     QR"
     gene            complement(2237..2743)
                     /locus_tag="AC16GM_04470"
     CDS             complement(2237..2743)
                     /locus_tag="AC16GM_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR2922966.1"
                     /translation="MTNEARMIKAKINSLALDVSTNSPVVVLSPEDSEKILPIWIGHY
                     EAWAIAMELSGIASKRPLTHDLVKAVVGSLGGKIQKVEVTDLVSQTFYAKIYIERNGS
                     VLTVDARPSDSIALALKSKAPIFVNETLFQEKKEETGERPEGESPDSLRERLRRINPE
                     DFGKYSLG"
     gene            complement(2822..3283)
                     /gene="rplI"
                     /locus_tag="AC16GM_04475"
     CDS             complement(2822..3283)
                     /gene="rplI"
                     /locus_tag="AC16GM_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR2922967.1"
                     /translation="MKIILRDDVEKLGKAGEVLEVKDGYARNYLLPRNLAIPATQGNL
                     RAIDQIHRQKAGREQKRQRESLKLKEAIEKVSLTAEVLVGEEDRLFGSVTALDIAELL
                     ARQGLTIDRHKIELEEPIKALGVYSVPVRLAHDVTAEVKLWVMKKEKEAEA"
     gene            complement(3295..3534)
                     /gene="rpsR"
                     /locus_tag="AC16GM_04480"
     CDS             complement(3295..3534)
                     /gene="rpsR"
                     /locus_tag="AC16GM_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR2922968.1"
                     /translation="MAEEFEPRRIKKSCRFCARGAEPIDYKNERLLRRCVTEQGKIVP
                     RRISGCCAKHQRRVATAVKRGRHLALIPFVAEVYR"
     gene            complement(3561..3914)
                     /gene="rpsF"
                     /locus_tag="AC16GM_04485"
     CDS             complement(3561..3914)
                     /gene="rpsF"
                     /locus_tag="AC16GM_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR2922969.1"
                     /translation="MLRYETTVIFDPLLEEAALQTEIDRITNQITAAGGSVVALQRWG
                     LKRLAYSIKKKKQGYYLHVVFEGPGPLPQELERNYRLSESILRFLTVRAEESGPAQPE
                     AAGGASAERQAVEVE"
     gene            complement(3985..>4141)
                     /locus_tag="AC16GM_04490"
     CDS             complement(3985..>4141)
                     /locus_tag="AC16GM_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR2922970.1"
                     /translation="APEKKEQVAEYVLDRFLPEEQKVKEEIVNRAVVAVEICLESGLQ
                     TAMNQFN"
BASE COUNT          879 a         1244 c         1055 g          963 t
ORIGIN      
        1 ctttgacacc gaagatacta acgagggcac tgaaaacgtt ggcttggtcg taataaactt
       61 cgacgaactc gtcgagctct ctctcaatgt aggaaatggc ccgtcttatt attcccaccg
      121 tgagatcagg aggtaaacga acaccggaat cccatcctct ggaatccctc atttctttgg
      181 actctcaacg gtacgggtaa ctctttggct gatacgagcg aaattaacaa aacgaccaga
      241 catgttccca ttaagggaca atgcggcgag ccgtgggatg gcacgcctag ccatgcgaaa
      301 atagtgagga tccttctcga tgccaatact cgcatatcct acagcttccg ctgccgcgat
      361 agttgaacct gcgcccatga acgggtccaa tactatgcct tcccccagcg gaagactcgc
      421 atgaacaatc tgacgcataa aggcctgagg tttcaaagac gggtgatttg ctaaaccacg
      481 ctcctctggg cgggttggac ctgagggaat cacatcacta aaggggatct ctgcggagac
      541 acgacgtaaa ccgcctgtgc tccatttccg taagttatcc tgtactctac cttcacaagg
      601 ttttcggaaa agaccccagg gctcccatct agctctgggc atcacggtta catctcggaa
      661 ttctacgtgc gcattcttag gtctgtctcc acctcgcagt gtggtgacca agcgaataat
      721 ctcaccacgc ttctcaaatc ctgcctcaat cataggctgg taaacaaggt gggatagcag
      781 agaatttgtc gcaataaaaa cgtgaccccc tggggtcaaa actcgaaaga ttaactgggc
      841 ccattctgtg aagaactgtc tgattgcgtt ttgatctgcc cgattcaata ttgtaaatcg
      901 agggagtggg ctccgctggc acccgtcaaa cgatggtgga aggcgccaaa caccgcctct
      961 acctctacgc agttttttct gctccgaagg gctgtactcc tttaacccat aaggtggatc
     1021 ggtaacaact gcatgtatcg aatgcggttc tctccgggct aaccatgaaa aacaatcatc
     1081 gaggataaga atatatcccg ccattcagac tcccgatatt tttttaatta gactgacacc
     1141 atgtagttta cgaaaaagaa aatactacat ctagacgtca tgtcaactca atcttcttga
     1201 gagacttaca cctaatcccg gttcccgttc tcttatgatt ccatggttcg ccataaaccc
     1261 ccatttccat ttaattcttg aagagcggcc ccaaagaact ttttatgtgg tatttccttg
     1321 caatgaatat tgggtaagca ggtttacctt tgacctttcc gctcaagcgc cagtgcctca
     1381 ttcaaactcc cgctccggta ccccgccaga tcgagcgtga cgtacaggaa cccgatttct
     1441 cggagtcgat ccgctatggc cgtacgaatg ccctcatcca aaaaacggga tagttcctca
     1501 ggttccacct caatgcgggc cacctgctcg tggtgtagca cccgaaactg ccggaagccc
     1561 agcgaacgga ggaagcgctc ggcctgtgcg atttgagcca gcttttccgg ggtcacccgc
     1621 tggccgtacg gaacgcgcga cgagaggcag gccatggccg gcttgttcca gaagggcagg
     1681 tcatgcgctt tggcaatctc ccggatttcc ggcttggtga gaccggcggc cagaagcgga
     1741 cttttcacat tgccgaaaat gtctgccggc tttttgccgg ggcgaaaatc tcccgcatcg
     1801 ctggcattga aaccgtcgac gatgaaggga atgtcgtact gcgcggcaac gggggtgagt
     1861 ttggtgtaca actcctcttt acagaaatag cagcgtgccg gggaattttg agcgtagttt
     1921 tcgctggcga cctcgtcggt ctcaaccacc agatgccgct ccgccagacc gagtaagcgg
     1981 gcgaagcttc tggcgctctc caattcctcg ggcgggtaac tggccgagtt ggccgttacc
     2041 gccaggaccc tctcattccc caaaaatcgg caggccgccc agaggagaaa cgcagaatcg
     2101 acaccgccgg agtaggccac cagaaccgac ccgagggtgc gaagttcctc cgccaggcgc
     2161 tggagctttt ccgcagacat tggctaaccg gaaaaaggcc cggggtgtac ccatccggtg
     2221 aaccccggtg gtgccatcat cccagcgagt acttgccgaa atcttccgga ttgatccggc
     2281 gcagccgctc ccgcagcgaa tcgggcgact cgccttcggg gcgctccccg gtctcctctt
     2341 ttttctcctg gaagagcgtc tcgttgacga aaatcggcgc tttcgacttc aaggccaggg
     2401 cgatggagtc ggacggccgg gcgtccacgg tgagaacgct gccgttccgc tcgatataga
     2461 ttttcgcgta gaacgtctgg ctgaccagat cggtcacttc cactttctgg attttgcctc
     2521 ccagggagcc caccaccgct ttcaccaggt cgtgggtcag ggggcgtttg gaggcgatgc
     2581 cggagagctc catggcgatg gcccaggcct cgtagtggcc gatccagatc ggcaggattt
     2641 tttcggaatc ttccggggag aggaccacca ccggcgagtt ggtggaaacg tccaaagcca
     2701 gactgttgat tttggccttg atcatccgcg cctcgttcgt catacgctcg tcttcaccaa
     2761 tggcttcgct cgctctatca atatatacgg cagcgatccg cgcttgcacc acgaaaaatg
     2821 cctacgcttc ggcctctttt tcttttttca tcacccagag cttcacttcg gcggtcacgt
     2881 cgtgcgccag ccggaccggc acgctgtaga cgcccaaggc ttttatcggc tcttccaatt
     2941 caattttgtg ccggtcgata gtgagcccct gccgggccag aagctccgct atgtccagcg
     3001 ccgtaaccga gccgaacagc cggtcctctt ctccgaccaa gacctccgca gtcagagaaa
     3061 ctttttcaat cgcctctttc agtttcaggg actctctctg tcgtttctgc tcgcgacccg
     3121 ccttctgccg gtggatttga tcaatcgcgc gaagattccc ctgagtggcc ggaatcgcca
     3181 aattacgcgg gagcaggtaa ttccgcgcgt agccatcctt gacttccagc acctcgccgg
     3241 ccttgcccaa tttttcgacg tcgtcgcgca ggataatttt cataggctct gcctctaccg
     3301 gtagacctcg gccacaaacg gaatcaaggc taggtgccgc ccccgcttga cggctgtggc
     3361 gacgcgcctt tggtgcttgg cgcagcagcc ggaaatccgc cggggaacga ttttcccctg
     3421 ttcggtgacg caacggcgca acagccgttc gttcttgtag tcaatcggct ccgcgccccg
     3481 ggcgcagaag cggcagctct ttttgattcg tcgtggttca aattcctcag ccatcgccac
     3541 tccttaagat cattcccgtg tcattcgact tctaccgcct gccgctcggc ggacgctccc
     3601 ccggccgcct ccggctgggc cggacccgac tcttcggcgc gcaccgttaa aaaacgcagg
     3661 atgctctcac tcaaccggta atttcgctcc aactcctgcg gcaggggccc cggcccctca
     3721 aaaacgacgt ggagatagta cccctgtttt ttctttttaa tcgaataggc caggcgtttg
     3781 agcccccagc gctgcagggc caccaccgac ccgcctgcgg cagtgatttg attggtgatc
     3841 cggtcgattt cggtttgcag cgccgcttct tctaagagcg gatcaaaaat aaccgtcgtt
     3901 tcgtaacgca gcactctggg tctcccttcg gtctctggcg gccccccggc ggatcaccag
     3961 acccgcctgg ggagcagggt acacctaatt aaactgattc atcgccgttt gtaaaccgct
     4021 ttccaggcaa atttctactg caaccacggc ccggttcaca atctcctctt taaccttttg
     4081 ttcttccggc aggaaccggt cgagaacata ctcagccacc tgctcttttt tttccggcgc
     4141 g
//
LOCUS       DBNGZD010000166         4390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1416318, whole genome shotgun sequence.
ACCESSION   DBNGZD010000166 DBNGZD010000000
VERSION     DBNGZD010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1416318"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16GM_04495"
     CDS             <1..208
                     /locus_tag="AC16GM_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR2922971.1"
                     /translation="IRPQSATVEGLDLDGKTVTYEGKGMVARVFLHELDHLNGVLFID
                     HLSPAKRELLKSRLREIKEKHWAL"
     gene            209..1138
                     /gene="fmt"
                     /locus_tag="AC16GM_04500"
     CDS             209..1138
                     /gene="fmt"
                     /locus_tag="AC16GM_04500"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR2922972.1"
                     /translation="MRSVFLGAPEFALPSLQKLAESHHTVAAVVTHPDRPQGRGLKPE
                     PTPVKRQAQKLGLPVLTPAKLDAPDFLEQMRGLQSDLMVVVAFRFLPEALYAIPPKGA
                     INLHASLLPKYRGAAPIQWVLIHGAKVTGLSTFFLNRSLDQGDLLLQREVAIAPDDNF
                     GSLSARMAEQGAGLLLETIDRIEAGTLKPKKQDNSQATRAPKITKEMCVIDWSRSAEK
                     IHNLVRGLAPEPGAVTFFQGKRLKILRTGITPEKETGSAGAILHADPKQGLSVAAGEG
                     SLELLEVQPEGKSPVSGAEFVRGYRPRTGERLG"
     gene            1222..2817
                     /locus_tag="AC16GM_04505"
     CDS             1222..2817
                     /locus_tag="AC16GM_04505"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR2922973.1"
                     /translation="MPEPTGTDNRPVLSKEIIINVSEHETRIAILEEGKLVELLVERA
                     EAERMVGNIYKGKVTNVLPGMQAAFIDIGAEKAAFLHASDAGEVVTGDEESEESADEA
                     EKPQRGRGDSDREDERRFRSIQEVVAKGQEILVQIVKEPISTKGPRVTTEISLPGRFI
                     VLVPAWKQVRVSKRIEGWPERRRLRRIVTELLPPGLGAIVRTEGEGKTEREFKADIKL
                     LVHTWKKILRQAARKGAPALIHKEFGITSSIIRDLFTRDVDRLVIDSRKEYKLILGYL
                     RSVAPELREKVSLYAESVPIFDLYGIESEIEKMLDRKIWIKRGAYIVIDQTEAVVAID
                     VNTGRFVGKGNQEQTIFRTNLEAAKEVARQIRLRDIGGLIIIDFIDMYSRENRRRLFE
                     EFRAGFKNDRSKFSISPVSEFGLIEMTRERVRPSLMYTFSEPCPTCSGFGRVLSRETV
                     ATKIERWFKRAKAGAKYKRFKLIISPYLAEAIVDANPSRVRQVAQEFKVEIDVIRDTT
                     MPPEEFRVIAAQDGKDITELFSA"
BASE COUNT         1009 a         1206 c         1358 g          817 t
ORIGIN      
        1 catccgcccc caatccgcga cggtggaggg actcgatctc gacggcaaaa ccgtaaccta
       61 cgagggaaag gggatggtgg cgcgggtctt tttgcatgaa ctggaccact tgaacggggt
      121 gttgttcatc gaccacctga gcccggccaa gcgtgagctc ctcaagagcc gcctgcggga
      181 gatcaaagag aaacactggg cgttgtagat gcgctcagtc ttcctggggg cgccggagtt
      241 tgcgctcccc agtttgcaaa agctggccga aagtcaccac accgtggcgg cggttgtcac
      301 ccaccccgac cggccgcagg gccggggttt gaagccggag ccgacgccgg tcaagcggca
      361 agcgcagaaa ctggggttgc cggtgctcac ccccgcaaaa ttggatgcgc ccgattttct
      421 ggaacagatg cgcggtttgc agagtgacct tatggtagtc gtcgcctttc gcttcctgcc
      481 ggaagcactc tatgccattc cgccgaaggg cgccatcaat ctccacgcgt cgcttctgcc
      541 caagtaccgc ggggcagcgc cgattcagtg ggtgctcatt cacggagcaa aggtgaccgg
      601 tctctcgact tttttcctta accgcagtct cgatcaaggc gaccttttgc tccagcggga
      661 ggtggcgatt gcgccggacg acaacttcgg gagtctttca gcgcgcatgg ccgagcaagg
      721 cgccggactt ctcttggaga ccattgaccg tattgaagca ggtaccctca aaccgaaaaa
      781 acaggacaat tcacaagcca cccgggcgcc gaagattacc aaagaaatgt gcgtcattga
      841 ctggtcgcgt tccgccgaaa agattcacaa tctggtgcgg ggtttggcgc ccgagccggg
      901 cgccgtcacc tttttccaag gcaaacggct gaagattttg cggacaggca tcacgccaga
      961 aaaagaaacc ggttcagccg gtgccattct ccacgccgat ccgaagcagg gtctctccgt
     1021 cgctgccgga gagggaagtc tggaactctt ggaggtgcag ccggaaggga agtcacctgt
     1081 ttccggcgcg gaattcgtgc gcggctaccg tccccgaaca ggggaaagat tgggatgaac
     1141 agagttgaaa ccgtcagaac taaagacgcg ggtcagccac cggcacgccc gccggcgccc
     1201 gcgtcccacg tgaaaacacc tatgcctgaa ccaaccggaa cagacaaccg ccccgttttg
     1261 agcaaagaaa tcatcatcaa cgtctccgag cacgagaccc gcattgccat cctggaagaa
     1321 ggcaagttgg tcgaactttt ggtcgaacgg gccgaagcgg agcggatggt ggggaacatt
     1381 tacaaaggga aagtcacgaa cgtcctcccg ggaatgcagg cagctttcat cgacatcggc
     1441 gccgaaaaag ccgccttttt gcacgcgagc gacgcgggcg aagtggtgac cggcgacgag
     1501 gagtccgagg aatcggccga cgaagccgaa aaaccgcagc gcggccgggg tgactccgac
     1561 cgggaagacg agcgccgctt ccggagcatt caggaggtgg tggcgaaggg tcaggaaatt
     1621 ttggtgcaga tcgtgaaaga gccgatcagc accaaagggc cgcgggtcac caccgaaatt
     1681 tcgctccccg gccgcttcat cgtgctcgtg cccgcctgga agcaggtacg ggtctccaaa
     1741 aggattgagg gctggccgga aaggcgaagg ctgcggcgca tcgtgaccga acttctgccg
     1801 ccgggcctgg gcgccattgt ccgcaccgaa ggcgagggga agaccgaacg ggagttcaaa
     1861 gccgacatca agctcttagt ccacacctgg aagaaaattc tgcgccaggc ggcgcgcaag
     1921 ggagcgcccg ctttgattca caaagagttc ggcatcacct ccagcatcat ccgcgacctc
     1981 ttcactcgcg acgtggatcg gttagtcatc gattcccgaa aagagtacaa gttgatctta
     2041 ggctacctgc ggagcgtggc gccggagctg cgcgagaaag tttccctcta cgccgaatcg
     2101 gtgccgatct tcgatctcta cggcatcgag tcggaaatcg aaaaaatgct cgaccgcaag
     2161 atctggatca aacgcggggc ctacatcgtc atcgaccaga ccgaggcggt ggtggcgatc
     2221 gacgtcaaca ccggccgctt cgtcggcaag gggaaccagg agcagacgat tttccgcacc
     2281 aacctggaag cggccaagga ggtggcgcgg cagatccggc tgcgcgacat cggcgggctc
     2341 atcatcatcg acttcatcga catgtactcg cgggagaacc ggcggcggct cttcgaagag
     2401 ttccgcgccg gcttcaaaaa cgaccgctcc aagttttcca tatcgcccgt ctcggaattc
     2461 ggcttgatcg agatgacccg cgagcgggtg cgtccctctt tgatgtacac cttctccgag
     2521 ccctgcccca cctgttccgg ctttggccgg gtgctctcgc gggagacagt ggcgactaaa
     2581 atcgagcgct ggttcaagcg ggccaaagcg ggagcgaaat acaaacgctt caaactgatc
     2641 atctcccctt atctggccga ggccatcgtg gatgccaacc ccagccgggt gcggcaggtg
     2701 gcgcaggagt tcaaagtgga aatcgacgtg atccgcgaca ccacgatgcc gccggaagag
     2761 ttccgggtga ttgcggcgca agacggcaag gacattacgg agcttttctc ggcataaagc
     2821 gaactccgtg tcggggtgtc gaggttaagg cggaaccaaa gttcctggga ggggggcaaa
     2881 acggcataaa ttttttcttt tgcgtcctgg ttgaacggca acgagatccg cagaaaagcg
     2941 gggaaaagcc gtttggcggg aaaactgcgc aactctctac cgggcaaggg gcagcggcag
     3001 ttcggggaag taaaccggtt ttcacgataa aaagcgcaat cgggcggagc aaggtcttat
     3061 cgtccaagag aataatcacg gttcggggga gcggcacgga aattcactca tcgtcagggg
     3121 cgacgtacca gcggcagttg cgcaagcgta aagcggtctt ggggttaatg tcacagaggg
     3181 cggcccaagg gtgtcgaagt gtcaaggtgt cggggtgtcg gtggagcagg agtaagaaca
     3241 aagcaaggtg gtaggtggaa ggtgataggt ggtaggaaaa aaatggattc gggagatggg
     3301 agttaggatt tgaggaaaag caacggcatt cacagcagat ggagcagata aaaagctctc
     3361 accacggaga cacggggggc acggagaact gacaacggat tagccagcgg attaagcggg
     3421 ttatctcatc tgcctgtgtc tttctattcc ttctcttccc ctgggttttc caaggtggcc
     3481 acgtggcctt tttctcctgg cgagagtgat gcccgatcct atccgcctga ggcgggtcgg
     3541 accgaccggc gtgcgctggc gcttgaagct ctttgtttaa cgggtacccg gtcccgcctt
     3601 tggacgggat cttcgacctg aagttggcca gctcacccgg tacgggtcta ccgacagagg
     3661 ccggtgagag cactcaggcc ctctactatt tcgcactttg tccgttttac ccccaagacg
     3721 accccaaatt ttctctctcc gcgtagacgg cactttcagt gccgtccctt ttgtcgggac
     3781 taaaagtccc gcctacgcga aaggaagagg gcatccgttt tacctccatg gcaaccccaa
     3841 attagcgggg gaaggggcac ctgtgcgccc ccatgacgac cccaaattag cgaggctgga
     3901 agcctcgcct atggggagag gacatccgtt ttacccccgc gacaacccca aatttctgag
     3961 acatggggga ttagggatca gtaaagaaaa gaactggact caagtcccgc ctacgcgggg
     4021 agagggaaga agcggacgaa tccccttagg cgaattgaac cactcctgta ttgaactttc
     4081 cttgacagac aaaagaatcc gaatttattg attgtggaat aggtggggat gcctcgagga
     4141 ggaaagaggc gtgataagaa aacggggggg caaattagtc ttttgaaacc agcgggtcga
     4201 gatgacccga tgggagggtg tatgcgcaga gcgctacgat ttttgaccgc taccgctcgt
     4261 aagagtcgaa gccgcccata cgggccgtat ggcctgaggc ggatttggac gagtctgacg
     4321 acagttctgt tcgtttatgc cagcgtgggc tacagccagg tgccggtcct gctttaccag
     4381 aaaaacggcg
//
LOCUS       DBNGZD010000167         6683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1486149, whole genome shotgun sequence.
ACCESSION   DBNGZD010000167 DBNGZD010000000
VERSION     DBNGZD010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1486149"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1200
                     /locus_tag="AC16GM_04510"
     CDS             52..1200
                     /locus_tag="AC16GM_04510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2922974.1"
                     /translation="MKSKADVVVIGGGVIGAAVAYNLAKRGLKEVVVLEKEPFLASGS
                     TGKAAGGIRAQFSTPGNILFSMRSEELFACFPEEMDSPAQFEQVGYLFLVSDEKTWEI
                     YKQQAELQQKLGLSVQLLTPEEALQLAPFVRMSDLVGATFCGRDGLGDPGDFSQGYIT
                     QARRLGVEFALEAEVTGIEVTGGKVGGIISQKGRIATQVAVNAAGPYALQIGKMVGVD
                     IPVLPLKRQVVTTAPLPYVPPSLPMVVDVATGLYLHKESGGLLLGWADRDTPPGFDES
                     VDPNYSLAIMERALHRIPQLGEAQISSEWAGLYETSPDHNAILGRVPEVEGFICANGF
                     SGHGFMHAPAAGETIAELILEGKSSLDISAFSIERFRNGNFAVEQNVI"
     gene            1500..3509
                     /locus_tag="AC16GM_04515"
     CDS             1500..3509
                     /locus_tag="AC16GM_04515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2922975.1"
                     /translation="MDDRLQKIQKRDWQLWALALFVLFAAVSLVVFLALGTNIGQWFQ
                     KEFRMQNVLFLLLDFALLAILLGIYLVQEEVKLKKIQEHLIEEQVSSASWQHQFQELR
                     ALFEVSTVMSAKVELPEILNTICEQVNTSLGADRTSVMLYDEHKRTLSCAAAAGQKKA
                     LTVNQEVALGKSVAGWVMEHGEAVVLGDDLSHFAFAEFVPKEEKIASAICIPLVIKGK
                     RAGVLSASRFNPQRKFSPEDLKIVSIFAENAAVSIERAQLYREVKESTRQLVQTEKMR
                     ALGEMAAGVARDFNNTLAIILGRVELLMQQIQDPKYAGWLESVRHAGLHASEIVKKIQ
                     DFTRRHATKTFVSLDLNQIIRDTVELAAPKWKHEPAARGKTIHLSTGFGEISPVEGNP
                     SELKEVFMNLIFNAVEALPEGGTILFQTRLVQGQVEVTVIDNGVGMSEETKQKIFEPF
                     FTTKGPKGTGLGLSVAYGIVAHHGGEIAVDSALGKGTTVCIRLPVHKPAHEQTKVEPK
                     VPKSQELSGARIVIVNGEAPENTILTNFLLGCGHLVKSVLSGREVLDQLRRDDLDLVF
                     LDSALSKSSPFELAAEMKRTKSAPAVVLIGGWGHVRLTQDATEQGIDFVIGRPFVLDE
                     ISAVTQKALLQRRGRLLREQVIPSRTDRAPADPVIDLTKTGAEVV"
     gene            complement(3543..4646)
                     /locus_tag="AC16GM_04520"
     CDS             complement(3543..4646)
                     /locus_tag="AC16GM_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922976.1"
                     /translation="MLCLSLAFACGQKKKNAAEENPAPASTGGPSQFQDTSSIRIAPA
                     SPAPVPQAHSQSEARGLNLPGRIAFWRLGQLWVMNADGSNQRQILRFPTGDNGYGKIS
                     WAPDGKRLAFARKGLLTVNYPDGGTGNVPIYDVFYTHLDSANWFFGLSDEFGSSFPDW
                     SPDGSFVVYARDMNRATAAANLAQYQPNYQLVVEQVKNYTTRTLQMPKSTQTPLQAIQ
                     PAIAPDGRRVAFSVVISSPQGGGEVVGIATDDLRGIKKSGDALLSEARRTPGGYGPAW
                     SPDGKQLAFIVTQGATQGLCLMAPDGTNQRLLVEKSDRISINSFPPAWSPDGRWLAFS
                     TADSSIYVVNVQSRETKRLTGPHDDLHPAWSKK"
     gene            complement(4816..5652)
                     /locus_tag="AC16GM_04525"
     CDS             complement(4816..5652)
                     /locus_tag="AC16GM_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922977.1"
                     /translation="MPGSNLKKPLTEKDIVSHKVKELEGKHEGASGTLGFFDLTFGGG
                     APLLARYNNQFRAIAWKNIFRSDGSSQRLFEPINSSLDLVGSLGLTFGRHMVSGNFSY
                     WFKMGSANVGDFVLPQPPNGTNTLVTNFPLTSEITAWGISADYQLFVLNPPGEHPLGR
                     TWSFRLGGGAGYYHASWNLWNGYGATDPTTGQYAVAPLPLTQSKFAPHLSLGGDVPVG
                     QGWVLSVDTRYLFLNFNKIGWNDPSGNFHYVEYPSQPGERIPLDLSGFRAHLGFKKYF
                     SF"
     gene            5720..6385
                     /locus_tag="AC16GM_04530"
     CDS             5720..6385
                     /locus_tag="AC16GM_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922978.1"
                     /translation="MTPPAGLGAAGGGAGIGEVGAGEVDGLGVTVGVGVTGFGIAVVA
                     GVPDGFGVSVGLGATGLAVALGFGGAAFGVVGVAGLAAGDGGVAGLGAAVVVGVPAGF
                     DISAGFGATVEVTEPVGFGAFGLAGTVVAGVPEGCLGAGAAAVPVDFGAGGGLEVAAG
                     TRLPGFGVAVVAGVPVGFAVAAGFWAGFWVVAGVAVPAGLGTSVALGAAVAAGVPVGL
                     GAV"
     gene            complement(6326..>6683)
                     /locus_tag="AC16GM_04535"
     CDS             complement(6326..>6683)
                     /locus_tag="AC16GM_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922979.1"
                     /translation="RPTSPARRYVQQHGDGSARRNDRPPRRRRTTRYDTTDRDGSGNG
                     NAKTGLTNKSSSASAYQTGSPTAENNCNSGAAETVNSAGQTSRTETGCAKSRSPNSNR
                     AQADRYSGCHGRTQSD"
BASE COUNT         1313 a         1781 c         2219 g         1370 t
ORIGIN      
        1 cctgcttgac tgtggccgcc gggagaaatt gattactcct cggagcggga gatgaagagt
       61 aaagccgacg tagtggtcat cgggggtgga gtgatcgggg cggccgtggc ctacaatctg
      121 gccaagcggg ggctcaagga ggtcgtcgtc ctcgaaaagg aaccatttct cgcttccggt
      181 tccactggca aggccgccgg cggtatccgg gcgcaattct ccaccccggg aaacattctt
      241 ttctccatgc gctctgagga gttattcgct tgcttcccgg aagaaatgga ctcaccggcc
      301 cagttcgagc aggtgggtta tctctttttg gtctccgacg aaaagacgtg ggaaatctat
      361 aaacagcagg cggaactcca gcagaaattg ggcctgtcgg tccagctcct cacgcccgag
      421 gaggcgttgc agctcgcacc ctttgtgcgg atgagtgatc tggtgggtgc gaccttctgc
      481 ggcagggacg gtttggggga tccgggcgac ttcagccagg ggtatatcac ccaggcccgg
      541 cggctggggg ttgaatttgc cctcgaggcc gaggtcaccg gcatcgaggt cacaggcggg
      601 aaggtgggcg ggatcatttc ccaaaagggg agaatcgcta cacaagtggc ggtcaatgcc
      661 gccggcccct acgcattaca gatcgggaag atggtgggtg tcgatatccc ggttctcccg
      721 ctcaagcgcc aggtggtgac gaccgcgcct ttgccgtacg ttccgccctc cttgccgatg
      781 gtggtggatg ttgccacagg tctctacctg cacaaggaat caggcggcct gctcctgggg
      841 tgggccgaca gagatacgcc tccgggattc gacgaaagtg tcgacccgaa ctacagtctg
      901 gcgattatgg aacgggcctt gcaccggatt ccgcagctcg gagaagcgca gatttcgtcg
      961 gaatgggctg ggctgtacga gacctctccc gatcataacg ccatcttagg cagggtaccg
     1021 gaggtggagg ggttcatctg cgccaacggt ttttcgggcc acggtttcat gcacgctccg
     1081 gcagcggggg aaactattgc ggagttgatc ctggagggca aatcgtctct ggatatctcc
     1141 gctttttcca ttgagcgctt ccgcaacgga aacttcgctg tcgagcaaaa cgtcatctaa
     1201 gcactttctg gcagaggcgc accgtcaatt ccaaagacgg ttccccttct gatttttcga
     1261 gagcccgcaa agatgcctgc gggttttctc ggttccgact caaccggctg tagccgggga
     1321 gcgcaacgca gtcgttgaaa gacgggaaat cccattgacc agctggggag cgcgacgcac
     1381 cggccggagg cgctgaaagc gggggaaaat gcaggacact atggtgacgc ctccggattg
     1441 ttcatcgtca gggagaatat gccgatatac agagagactg agagaatcgg aacaaatcca
     1501 tggatgatcg tttgcagaaa atccagaagc gcgactggca gctttgggcg ctcgcacttt
     1561 ttgtcctgtt cgcggccgtc tctctggtcg tctttttggc gcttggcacg aacatcggcc
     1621 agtggtttca aaaagaattc cggatgcaga atgtgctgtt cctccttttg gatttcgccc
     1681 tcctggcaat ccttttgggc atctacctgg tgcaggaaga ggtaaaatta aaaaaaatcc
     1741 aggagcacct gattgaggaa caggtcagtt cggcgtcgtg gcagcaccag tttcaggaac
     1801 tgcgggcgct ctttgaagtc tccacggtca tgagcgccaa agttgaatta ccggaaatcc
     1861 tgaatacaat ttgtgaacag gtgaacacga gtctgggagc cgaccgcacg agcgtcatgc
     1921 tctacgatga gcacaaacgc acgctcagct gcgcggctgc agccggccag aagaaggccc
     1981 tgacggtgaa tcaggaagtg gccctgggga agtcggtagc gggctgggtg atggagcacg
     2041 gcgaggcggt ggttctgggc gatgaccttt cgcacttcgc gtttgccgag ttcgttccca
     2101 aagaggagaa gatcgcctca gccatctgca tcccgctcgt gatcaaaggg aagcgggcgg
     2161 gggtgctcag cgcgtcgcgg tttaaccccc agcggaaatt cagcccggaa gacctgaaaa
     2221 tcgtttccat ctttgccgag aacgccgcgg tctccattga gcgagcgcag ctgtaccggg
     2281 aggtcaaaga atcgacgcgg cagctggtcc agacggaaaa gatgcgggct ttgggggaga
     2341 tggcggccgg ggtcgcccgc gatttcaaca atacactggc gatcatcctc gggcgggtcg
     2401 agctcttgat gcagcaaatc caggatccta agtacgcggg atggctggag agcgtccggc
     2461 acgccggcct gcacgcgagc gaaattgtca agaaaattca agatttcacg cggcgccatg
     2521 caaccaaaac gttcgtatcc ttagatctca accagatcat ccgggacacc gtcgaactcg
     2581 ccgcgccgaa atggaaacac gaaccagccg cgcgcgggaa aaccattcac ctctccacgg
     2641 gcttcggtga aatctcccca gttgagggaa acccgtcgga actcaaagaa gtgttcatga
     2701 acctgatttt caatgcagtg gaggcactcc ccgagggagg caccattctc ttccaaaccc
     2761 ggttggtgca ggggcaggtt gaggtgaccg tgatcgataa cggggtgggc atgagcgaag
     2821 aaaccaaaca gaagattttt gaaccgttct tcaccaccaa aggccccaag gggaccggtc
     2881 tcggcctgag tgtcgcctac ggcatcgtgg cgcaccatgg aggcgagatc gccgttgatt
     2941 ccgctctcgg caaaggaacc acagtttgca tccggttgcc cgttcacaag ccggcgcacg
     3001 aacaaacgaa ggtggagccg aaagtcccaa aaagccagga attatcgggt gcacgcatcg
     3061 tgatcgtgaa cggcgaggca cccgagaaca cgattctgac gaacttcctt ttgggttgcg
     3121 gtcatctggt aaaaagtgtc ctttcgggac gggaggtact cgaccaattg aggcgggacg
     3181 acctggatct cgtattcctc gactccgccc tgagtaaatc gtcacctttc gaacttgccg
     3241 ccgaaatgaa gagaacgaaa agtgcacccg cagtggttct catcgggggg tggggacacg
     3301 tccgcttgac ccaggacgct accgagcagg ggatcgattt tgtgattgga cggccgtttg
     3361 tgctggatga gatttcggcc gttactcaga aagccctgct tcagaggcgg ggccggctcc
     3421 tccgcgagca ggtcattccc tcccggaccg acagggctcc cgcggaccca gtcatagatc
     3481 tcacgaagac cggagcggag gtagtttaac ctccggaact tcgttccacc ccagaaagcc
     3541 gactacttct ttgaccaggc ggggtgcagg tcgtcgtgcg ggccggtaag ccgtttggtt
     3601 tcgcgcgact ggacgttgac gacgtagatg ctcgagtcag cggttgaaaa agcgagccac
     3661 ctgccatccg gggaccaggc gggcggaaaa ctattgattg aaatgcggtc gcttttttcc
     3721 acgagcaagc gctggttggt accatccggg gccatcagac aaaggccctg agttgccccc
     3781 tgggtgacga taaaggcgag ctgtttccca tcgggcgacc aggccgggcc gtagccgccg
     3841 ggcgtccggc gtgcttcact caaaagcgcg tcgccacttt tcttgatacc gcgcaaatcg
     3901 tctgtggcaa tcccgaccac ttccccgcca ccttgtgggc tggaaatgac cacggagaag
     3961 gccacacggc ggccatccgg ggcgatggcg ggttgaatcg cctgcagcgg cgtttgagta
     4021 gatttcggca tctggagcgt tcgggtggtg tagtttttta cctgttccac cacgagctgg
     4081 tagttgggct ggtactgcgc caggttcgcg gcggcggtgg cccggttcat atccctggcg
     4141 tagacgacga aagaaccatc cggcgaccaa tcgggaaagc tggaaccgaa ttcgtcggag
     4201 agtccgaaaa accagttggc tgaatcgaga tgggtgtaga aaacgtcata gatgggaacg
     4261 ttgcccgtac cgccatcggg gtagttaacc gttaaaagtc ccttacgagc aaaagcgagc
     4321 cgtttgccgt cgggtgccca ggagattttg ccatagccgt tgtcgccggt cggaaagcgg
     4381 aggatctggc gctggttgga gccgtcggca ttcatcaccc agagctggcc caaccgccag
     4441 aaggcgatgc ggccggggag gtttaacccg cgcgcctcac tttgactgtg cgcctgcggc
     4501 accggtgcgg gtgaagccgg agcgatgcgg atgcttgaag tgtcctgaaa ttgcgacggc
     4561 ccgccggtgg aagccggagc gggattctcc tcagccgcgt ttttcttttt ctgtccgcag
     4621 gcgaaagcga gagagaggca cagcaacgct gggcccagac cgaggaaagg tcgtcccgga
     4681 agagtcatcg cggcggcaaa tatagtcgaa ccggggagca gcacaacaac aaaatttcgg
     4741 gtacaagctg atgggtaaaa gaagacgggc tcggagacga gcccgtctac ccttgcggag
     4801 gaaaaactcc cgcgtttaga aggagaagta cttcttaaat ccgagatgcg cccggaagcc
     4861 gctcagatcc aatggaatcc gctcgcccgg ctggctgggg tactcaacgt agtggaaatt
     4921 gccggaaggg tcgttccagc caattttgtt gaagttcagg aacagatagc gcgtgtccac
     4981 cgagagcacc cagccctggc cgacggggac gtcgccgccc agactcaggt ggggggcaaa
     5041 cttgctttgc gtgagtggca ggggtgccac cgcatactgg ccggtggtgg ggtctgtggc
     5101 cccgtagccg ttccaaagat tccaggaggc gtggtaatac cccgcaccgc cgcccagccg
     5161 gaaagaccac gttcggccca gaggatgctc gccgggagga ttcagaacga agagctggta
     5221 gtcggcggag ataccccagg cggtaatttc gctggtcaac gggaagtttg tcaccagcgt
     5281 gttggttccg ttcgggggct ggggcaaaac gaagtcgccc acattggcgg aacccatttt
     5341 gaaccagtac gagaaattcc cggaaaccat atgccgtccg aaggtcaggc cgagactgcc
     5401 gaccaagtcg aggctggagt tgatcggctc aaacaggcgt tgcgatgagc cgtcggaacg
     5461 gaagatgttc ttccaggcga tggcccggaa ctggttgttg tagcgggcca acagcggtgc
     5521 gcccccaccg aaagtaaggt cgaaaaatcc gagcgtcccg cttgctcctt cgtgtttgcc
     5581 ttcgagctct ttgactttat gggatacaat atctttttcc gttaagggct tctttaagtt
     5641 tgagccaggc acaataccct ggtcgggctt gcgcggagcc agtggtgtgg cctggacagg
     5701 ggccggtgct ggggctggtg tgacgccccc ggcaggcttg ggtgcggctg gcggaggggc
     5761 cgggattggt gaagttggcg caggtgaagt cgacggtttg ggagtcacag ttggcgtcgg
     5821 ggtgaccggc tttggaattg cggtcgtagc cggtgtgccg gacggtttcg gtgtttccgt
     5881 aggtttgggc gccacgggtc tcgctgtcgc gcttggcttc ggcggagccg cctttggagt
     5941 ggttggggtc gccggacttg cggctggtga cggcggagtc gccggtttgg gagccgccgt
     6001 cgtcgtcggg gtaccggctg gcttcgacat ctcggcaggt tttggcgcaa cggttgaggt
     6061 taccgaaccg gtgggtttcg gcgcattcgg cttggccgga actgtagttg ccggcgtgcc
     6121 ggaaggatgt ttgggcgcag gtgccgcggc ggtgccagtt gacttcggtg cgggcggtgg
     6181 attggaggtc gcggccggca ccaggttacc cggctttgga gttgccgttg tggccggggt
     6241 gccggtcggc ttcgccgttg ccgcaggttt ctgggcaggt ttctgggttg tagctggagt
     6301 cgcggtcccg gctggtttgg gtacttcagt cgctttgggt gcggccgtgg cagccggagt
     6361 accggtcggc ttgggcgcgg tttgagttgg ggctccggct tttggcacac ccggtttcgg
     6421 tacggctggt ttggccggcg ctgttgacgg tttcggcggc gccggagttg cagttgtttt
     6481 cggcggttgg agacccggtt tgataggcgc tggcggagga ggatttgttg gtgaggccgg
     6541 ttttggcgtt accgttgccg gagccgtccc ggtcggtcgt gtcgtagcgg gttgtccgcc
     6601 ggcggcgggg tgggcggtcg ttccggcggg ctgacccgtc cccatgttgt tggacgtatc
     6661 gacgggcggg gctggttggg cgt
//
LOCUS       DBNGZD010000168         2254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_514521, whole genome shotgun sequence.
ACCESSION   DBNGZD010000168 DBNGZD010000000
VERSION     DBNGZD010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_514521"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..864)
                     /locus_tag="AC16GM_04540"
     CDS             complement(118..864)
                     /locus_tag="AC16GM_04540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR2922980.1"
                     /translation="MSAVKVVLFDLGSTLLEYENVPWETLDRRCIASGYRYLAEAGRP
                     LPPAEQFAAVFFDRFRAMWRETERTLAEIDLARFAEEVLQSLGITWRDGEKAGFFERY
                     YRPIFEQITPYPESAAVLESLSRADLRLGLVSNSIFPAQFHRQELQKFGLARFFDVTI
                     FSSEFGWRKPKPEIFQTALEQLRALPQEAIFVGDRVREDIWGPQQVGMRAVLKWRADR
                     EWSAGTVPDAIIHSLVELPAVLEVLNRTKV"
     gene            complement(851..1903)
                     /locus_tag="AC16GM_04545"
     CDS             complement(851..1903)
                     /locus_tag="AC16GM_04545"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR2922981.1"
                     /translation="MKILVLLGGDSAEREVSLASGEAVVKALQKQGHQVIAADPAVGL
                     KRLSDTNSILLGRIKKEPPAPEKLPVATEKTVIQTVESFDFEESDVIFLALHGGKGED
                     GTLQALLDMTGKPYTGSGMLASALAMNKAVSKRLFVQARIPTPHWLLCDTKRSRNLDQ
                     IVEEIRAGFAFPLVVKPHNQGSTVGLSVVHAPSELKPALETAAKFSPEVIVEEFIAGR
                     EITVGILGERALPVVEIIPQHEVYDYECKYTKGRSDYVCPAELTKEEAEEVQNLGLRA
                     FQCLGCSGYGRVDFRMSREGKFYCLEVNTLPGMTALSLVPMAAKGAGLDFPHLLEAII
                     EQALMYKKSGAAVVRS"
     gene            complement(1900..>2254)
                     /locus_tag="AC16GM_04550"
     CDS             complement(1900..>2254)
                     /locus_tag="AC16GM_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR2922982.1"
                     /translation="SHWRTPAHLAKVERWFVRYGAKILIASRFLSGIRSGIALAAGIG
                     NIGWRRMVLYGTVSILLWNGLILFVSSFVEQNWQRLYGLLLLYNKVLFGGLLLTAIGF
                     GIWWWRKRQVPKGAA"
BASE COUNT          491 a          736 c          566 g          461 t
ORIGIN      
        1 ccatcacccg ggccaccgcc tgttccttgc ccggcacgcc cggcgcgtcg gtcaattccc
       61 gtaaaaccgt ctcgctgaaa tccatcttcg ctcctctatc tctgcttttt acgattctta
      121 cactttcgtt cgattcaaca cttccaaaac cgccggtagt tcgaccaggg aatggataat
      181 ggcatccggc accgtgccag ccgaccactc gcggtcggcc cgccatttga gcaccgcgcg
      241 catcccgacc tgctgcggcc cccagatgtc ttcccgaacc cggtctccta caaagatcgc
      301 ttcctgtggg agcgcgcgca actgctccag agcggtttgg aaaatttccg gcttgggctt
      361 gcgccagccg aattccgaag agaaaatcgt aacgtcaaag aaacgagcca gcccgaactt
      421 ctgtaattcc tgccggtgga actgggccgg gaaaatcgag ttcgacacca gccccagccg
      481 caggtctgct cgggagagcg actccagaac tgcggcactt tccgggtagg gcgtgatttg
      541 ctcaaaaatc ggccggtagt accgctcgaa gaaacccgcc ttctccccat cgcgccaagt
      601 gatccccaaa ctctgcaaaa cttcttccgc aaagcgggct aagtcgattt ccgccagcgt
      661 cctttcggtt tcccgccaca tcgcgcgaaa acggtcaaag aaaacggcgg caaactgctc
      721 cgccggaggc agcgggcggc cggcctcggc caaataccga tacccggaag caatacaccg
      781 ccgatctaag gtttcccaag ggacattttc atactccaag agcgtggagc ccaaatcgaa
      841 aagaaccact ttaactgcgg acaacggctg ctccactttt tttatacatc agcgcctgct
      901 cgattatcgc ctccaaaagg tgcggaaagt cgagcccggc ccccttggcc gccatcggca
      961 ctaaggaaag cgcggtcata cccggcaagg tgttgacctc caagcagtag aacttccctt
     1021 cccggctcat ccggaaatcg acccgtccgt agccggagca gcccaggcat tgaaaggccc
     1081 gcagtcccag gttttgaact tcctcggcct cttccttcgt caactccgcc gggcaaacgt
     1141 aatccgaacg tccctttgta tacttgcact cgtaatcgta cacttcgtgc tggggaatga
     1201 tctccaccac tgggagcgcc cgctcgccca ggatgcccac ggtgatttcc cggccggcaa
     1261 tgaattcttc cacaataacc tctggagaaa acttcgccgc cgtctccaag gccggcttga
     1321 gttcgcttgg cgcatggacg accgaaagcc ctaccgtcga gccctggttg tgcggcttga
     1381 ccaccagagg gaatgcgaag cccgcccgga tttcttccac aatttggtcg agattacggc
     1441 ttcgtttcgt atcgcacagc aaccagtgcg gagtgggaat tctggcttgt acaaaaagac
     1501 gcttggaaac cgccttgttc atcgccaggg cactggccag catcccggaa ccggtgtagg
     1561 gcttgccggt catatccaaa agcgcctgga gcgtgccgtc ttcccctttc cctccatgca
     1621 gagccaaaaa gatgacgtcg ctttcctcga agtcgaacga ttcgacggtt tgaattaccg
     1681 tcttttcggt ggccaccggc aacttctccg gcgccggcgg ctcttttttg attctgccca
     1741 aaagaattga gttggtgtcc gaaagccgct tcaatcccac cgccgggtcg gcggcaatca
     1801 cctggtgccc ctgcttctgc agggctttca ccaccgcttc acccgaggcc agggagactt
     1861 cccgctccgc cgaatcgccg cccaataaga ctaaaatctt cacgccgctc ctttcggtac
     1921 ttgcctcttc cgccaccacc agatgccgaa gcctatcgcg gtcagtagaa ggccgccaaa
     1981 caggacctta ttgtagagca aaagcaggcc gtagagcctt tgccagttct gctccacaaa
     2041 ggaagataca aaaagaatca ggccgttcca taatagaatc gagaccgttc catagaggac
     2101 catcctccgc cagccgatgt tcccgatccc cgccgctaag gcgatgccgg agcggatgcc
     2161 gcttaagaac cggctggcaa tgagtatctt ggctccgtac cggacaaacc agcgctcgac
     2221 tttggctaaa tgagccggcg tccgccaatg gctt
//
LOCUS       DBNGZD010000169         3521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1064738, whole genome shotgun sequence.
ACCESSION   DBNGZD010000169 DBNGZD010000000
VERSION     DBNGZD010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1064738"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="AC16GM_04555"
     CDS             complement(<1..361)
                     /locus_tag="AC16GM_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2922983.1"
                     /translation="MAVLATIKTTPGGLLQEIELLCQAQKYRQAAELLAQISPEESGP
                     PNAKVAYLRALTFHHAGEYRQALAAARETLGALSVSSDHDLVAKTQYVIGDTYLALGD
                     LKNAEPYLRDSVSHFRRV"
     gene            complement(391..696)
                     /locus_tag="AC16GM_04560"
     CDS             complement(391..696)
                     /locus_tag="AC16GM_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922984.1"
                     /translation="MRKLIWLIPVLLALLTVGLCLELTNAKGVPNANGKANLADWDEH
                     PWDIHGAYRIDTLAITNEYLILRIWTSPDSFYVITVPRPTARGLTDYGKRTRSPLER"
     gene            complement(1018..2079)
                     /locus_tag="AC16GM_04565"
     CDS             complement(1018..2079)
                     /locus_tag="AC16GM_04565"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HIR2922985.1"
                     /translation="MSKAKSLFEHLAHKPARWLSGDGPEAGIVLSSRVRLARNIAGLT
                     YPTVADSDTRRQVVDALRQAVEKSVLLKSGELITSGEITELDRNFLIERHLISPEFMK
                     EKDHHALYVGEDEALAIMVNEEDHLRIQAITSGLEIARTYELADQADVELAGTLAFDF
                     DRDFGFLTACPTNVGTGMRASVLIHLPGLVLTRGIDAVINRITKVGLAVRGFYGEGSD
                     VLGNLFQISNQTTLGRSEQDILDSLEKVTRQIIEYELSARETLVRDAKEQIEDKIWRA
                     YGILKYARVLSSAEVMNLLSAVRLGLGLGFIRDVSLGTINEILLLSQPSHLQKYHQKE
                     LSSNERDILRAELVRSKLA"
     gene            complement(2076..2585)
                     /locus_tag="AC16GM_04570"
     CDS             complement(2076..2585)
                     /locus_tag="AC16GM_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR2922986.1"
                     /translation="MLCQDCNKRKASFHITQIINNQKAMLNLCHECAEKRGIHNPIQG
                     MPFPLGELLASMTSTQPAGEKKRGKTADPKCPGCGMSFSEFGKTGRFGCGHCYSAFRP
                     QLTDLLRKIHGSNQHQGKSPSAKSEKLQPLREERKLQDELRRAIASEDFEKAALLRDR
                     LRTLTGKEL"
     gene            complement(2591..3274)
                     /locus_tag="AC16GM_04575"
     CDS             complement(2591..3274)
                     /locus_tag="AC16GM_04575"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2922987.1"
                     /translation="MLAASGIEKSYQTPHGLLHVLRGVDLTVAPGEMVAVVGASGVGK
                     STLLHILGLIDRPDSGGLVLDGRNVLELDDGRLAQFRNRAVGFVFQFHHLLAECTALE
                     NVMVPLLIRGDSLQTAGEAAYILLQEVGVAERTGHRPAELSGGEQQRVAVARALATDP
                     KVVFADEPTGNLDRATAQELHQLFVTLCREKKTAVVVATHNLELARQCDRVLRIVDGR
                     TQPVERERI"
BASE COUNT          712 a         1034 c         1029 g          746 t
ORIGIN      
        1 ctacccgccg gaaatgggaa accgaatccc tcaggtaggg ttcggcgttt ttcaggtcgc
       61 ccagcgccag gtaggtgtcg ccaataacgt actgggtttt ggctaccagg tcatgatcgc
      121 tgctgacgct taaggccccc agcgtttccc gcgccgcagc cagggcctgg cgatactctc
      181 ctgcgtggtg gaacgtcaac gcccgcagat aggccacttt tgcgttcggg gggccgcttt
      241 cctctggaga aatctgggcc aaaagctcag ccgcctggcg gtacttttgc gcctgacaga
      301 ggagctcgat ctcttgaagg agaccgcccg gggttgtttt tatcgtggct aaaacggcca
      361 tcctcttctc tactccctta aagaggaagg ttaacgttct aatggtgatc gcgttctttt
      421 accgtagtca gtgaggccac gcgccgtcgg ccgaggtacg gtaatgacgt aaaacgaatc
      481 gggactggtc cagatgcgca gaatcaggta ctcattcgtg atggctaagg tgtcaatgcg
      541 gtaggctccg tggatgtccc aggggtgctc gtcccagtcg gcgaggttcg cttttccatt
      601 cgcgttcggg acccctttgg cgttggtcaa ctccaaacag agcccaaccg ttagaagggc
      661 cagcaagacc ggaataagcc agatcaattt tctcatgata ctatcctcct cgcagtaact
      721 ttgagaagaa agaagcggaa cgcagacaaa atccttacgg acacagcacc tccaggtggg
      781 tagaacgacg aaggcaacct atccactaac aaggtacaat gagagcgcaa aacttgtcaa
      841 gacattttcc acttattgac gcaaaaaatc cgcattgtgc ttctgagaac gatgatggta
      901 ttattattga gaaattgaga ataatccgtt tgtccggcaa tgagttagca aacgaatttg
      961 ggaaaacgtt cacaaacaag agcagactcg tgaagtgcct gctccgcaaa atctgcgtta
     1021 tgccagtttg gaacggacca attcggcgcg caggatgtcg cgttcgttgg aggaaagttc
     1081 tttttggtga tacttctgca ggtgcgacgg ctggctcaac aacaggattt cattgatcgt
     1141 ccccagcgac acgtcgcgga tgaaccccag acccaaaccc agtcgtaccg cggaaagaag
     1201 gttcatcact tcggcggaac tgagcacccg ggcgtacttc aggatgccgt aggcccgcca
     1261 gatcttgtcc tcaatttgct ctttggcgtc acgcaccagc gtttcccgcg ccgacagttc
     1321 gtattcgatg atctgccgcg tcaccttctc caagctgtcg aggatgtcct gttcggaacg
     1381 gcccagggtg gtctggttgg aaatctgaaa gaggttcccc aggacgtcgc tcccttcgcc
     1441 gtagaagccg cgcaccgcca gcccgacctt ggtgatgcgg ttgatgaccg cgtcgatgcc
     1501 gcgggtaagc accaagccgg gcaggtgaat gagcaccgaa gcccgcatgc cggtgccgac
     1561 gttcgtgggg caggcggtga ggaaaccgaa atcccggtca aagtcaaacg ccaaagtgcc
     1621 ggccaactcg acatcggcct gatcggccag ctcgtaggtg cgggcgatct ccaggcccga
     1681 ggtgatggct tgaatccgca ggtggtcttc ttcgttgacc atgatcgcca gcgcttcatc
     1741 ctctccgacg tagagcgcgt ggtggtcttt ctccttcata aattccggcg aaatgagatg
     1801 cctttcgatc aagaaattac ggtccagttc ggtgatttct cccgaggtga tgagctctcc
     1861 gcttttcaag aggacgcttt tctccaccgc ctggcgcagg gcatccacca cctgccggcg
     1921 ggtgtcgctg tcggccacgg tggggtaggt taaacccgct atgttgcgcg ccagccgcac
     1981 ccggctggaa aggacgatgc cggcttccgg tccgtcgccg gagagccagc gggccggttt
     2041 gtgcgccaga tgctcaaaca aagacttcgc cttgctcaca attccttccc cgtcagggtg
     2101 cgcagccggt cccgcaggag ggcggctttt tcaaaatcct cggaggcaat ggcccggcgc
     2161 agttcgtcct gcaacttgcg ctcctcccgc aagggctgga gtttctcgct tttggccgac
     2221 ggcgattttc cctgatgctg attggaaccg tggatcttgc gcaagaggtc ggtcaactgg
     2281 gggcggaagg cgctgtagca atggccgcag ccgaaacgcc cggttttgcc aaattcagaa
     2341 aagctcattc cgcaacccgg acacttggga tcggcggttt ttccccgctt cttctctccg
     2401 gccggttgag tggaagtcat gctggccaag agttcgccca aggggaaggg cataccctgg
     2461 atgggattgt ggatcccgcg cttctcggcg cactcgtggc acaagttgag catcgccttc
     2521 tggttgttga tgatctgggt gatgtggaaa ctggctttgc gtttgttgca atcctgacag
     2581 agcataccct tcagatcctc tcacgctcta cgggttgggt ccggccgtcc acgattctca
     2641 acacccggtc gcactgccgg gccaattcca gattgtgcgt cgccaccacc accgcagtct
     2701 ttttctcccg acagagagtc acgaacaact gatgtaactc ctgcgccgtg gcccggtcga
     2761 gatttccggt gggctcgtcg gcgaacacca ctttcggatc ggtcgccagc gcacgggcga
     2821 cggctacccg ctgctgctcc ccgccggaga gttcggccgg ccggtggccg gtcctttcgg
     2881 ccaccccgac ttcctgcaac agtatatacg ctgcctcacc ggccgtttgc aggctgtccc
     2941 cgcggattaa gagcggcacc atcacgttct ccagcgcagt gcattccgcc aacaggtggt
     3001 ggaactggaa cacaaagccg acggcgcgat tacggaattg cgccagccgc ccgtcatcca
     3061 actccagtac gttccgcccg tcaaggacga gcccgcctga gtcgggccgg tcgatcaggc
     3121 ccaggatgtg caagagcgta ctcttcccga cacccgaggc gccgacgacg gcgaccattt
     3181 ctccgggagc gaccgtcagg tcaaccccgc ggagcacatg caaaagaccg tgcggggtct
     3241 ggtaactctt ttcgatgcct gaggcggcca gcaccacacg gtcgttttca acgtatccct
     3301 ccgcccggct tctgtcaaga ggtatcggcc tgttccggga actcctgaat tcggggctca
     3361 ctggtagcgg atggcttcgg ccgggaccag tgccgccgcg cggcgggagg ggtagagggt
     3421 ggcgaggaaa ctgatcccca gggcggcaac ggcgacaatt aaaaaatcgc cgggccggac
     3481 gtcaatcggc agcgtgttga tgaagtaaat ttccgcgggc a
//
LOCUS       DBNGZD010000170         1470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_651794, whole genome shotgun sequence.
ACCESSION   DBNGZD010000170 DBNGZD010000000
VERSION     DBNGZD010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_651794"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16GM_04580"
     CDS             complement(<1..299)
                     /locus_tag="AC16GM_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922988.1"
                     /translation="MRKTELISLVALLVLAVGANGWGQQCCRSTAFPPAGQYTFPTSA
                     TLQVQLGPNPPMTLQPLGPTMVRAGDPVPNPSGDVIQTEIVALSLMSADPIVGNY"
     gene            complement(700..>1470)
                     /locus_tag="AC16GM_04585"
     CDS             complement(700..>1470)
                     /locus_tag="AC16GM_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922989.1"
                     /translation="FEPGVPTCDSIEIIGCLVLCPAGDQPFQVRLRDQFGNPVSGWPK
                     ENVWLNFSDCPSICPCPDEAAWPLVYPEDTSNAGGIVTFRIGAGGCREHCTITLDIVR
                     AVGDTCKLTVNLVRTTDFNCDLTVSFEDVCIMKNCFFQGICLPDPLCFDFNCDGINSL
                     TDLVLLVRHVGHCCGQGCLPSPSLCFVLGDFNDDAVKDVVDVVSVINCVVFTNPPCDQ
                     CCADLNYDGIVDIVDIICLINCVVFTTQCPGCPFNWCP"
BASE COUNT          368 a          414 c          369 g          319 t
ORIGIN      
        1 tcataattgc ccactattgg gtcggcggac atcagcgaca gtgctacgat ttcggtctga
       61 ataacgtccc ccgaagggtt ggggaccggg tccccggctc gaaccattgt tggccctaag
      121 ggctgcagcg tcattggcgg attgggaccg agttggacct gaagggtggc gctggtggga
      181 aaggtatatt gaccggccgg aggaaacgcg gtagaccggc agcattgctg cccccacccg
      241 ttcgcgccga cggccagcac caaaagggct accagactaa ttagctctgt tttccgcatg
      301 ttccctctcc tttttggtta tcgtaggact catttcgcct tcactctggg gggataaaat
      361 aaaaaagcac ccacacaaaa tgccgccttt cgttggctgg tcgatcttgc gacaatatga
      421 aaattcagcc caccccactc ccatggcgtt tctccctgcg ctcctacctt gcaaacgacc
      481 gcttctcaaa tcctcttcct gcggtcggac acctccccaa ttgcggagga atctactcca
      541 ctatacctat tttgtcaagg aaaaaatttg gttgtggtta agaaattctt tgtgacaaca
      601 atcacaagca gtaccccctt cgggtagccg accgccatga aaaaagggag ggcgcagtct
      661 aaagagacta cgccctcttc tggtgtttct ccggctacct tacgggcacc agttgaacgg
      721 acaacctgga cactgggtgg tgaagaccac acagttaatc aggcaaatga tgtccacgat
      781 gtcgacgatg ccatcgtagt tcaagtccgc acagcactgg tcgcacggcg ggttggtgaa
      841 aacgacgcaa ttaatcactg ataccacatc cacgacatct ttaaccgcgt cgtcgttgaa
      901 atcgcccagc acaaagcaaa gtgatgggct gggcaaacaa ccctggccgc aacagtggcc
      961 gacgtgtctg actaaaagaa cgagatctgt taggctattt atgccgtcac agttaaagtc
     1021 aaaacacaaa ggatccggaa ggcatattcc ctgaaagaag cagtttttca tgatgcagac
     1081 gtcctcgaat gagaccgtca gatcgcagtt gaagtcggta gttctgacca agttgaccgt
     1141 caacttgcag gtatcaccca ctgctcggac aatgtccagt gtaatcgtgc agtgctctcg
     1201 gcaaccccct gcccctatcc gaaaggttac aattccaccg gcattcgagg tatcttccgg
     1261 ataaaccaaa ggccacgccg cttcatctgg acacggacag atggacgggc aatcggagaa
     1321 gttaagccag acattctcct ttggccagcc gctcaccgga ttgccgaact ggtcgcgcag
     1381 tctcacctgg aacggctgat cgccagccgg acagagcacc agacagccaa tgatctcaat
     1441 cgagtcgcag gtaggtactc ccggttcaaa
//
LOCUS       DBNGZD010000171         4554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_861177, whole genome shotgun sequence.
ACCESSION   DBNGZD010000171 DBNGZD010000000
VERSION     DBNGZD010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_861177"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="AC16GM_04590"
     CDS             <1..986
                     /locus_tag="AC16GM_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR2922990.1"
                     /translation="KLKVLKKFFQPEEAPALLNSLQPVLNGRITIEKLDVLGYRLELR
                     SRSLLRLDLKQDSRTTTLVAKVMRPERAAQLFWHLQELERSGFHTRAADAITIPHPIA
                     YAPEGIIWLEAVFDPSLHDVIGEEAFVSGCGAAARTLNKLKTAPLENLVPYTLEEELL
                     LLRQTGAEIAQIYPVLAERLQEVLALVLQNAPAARQNRPAPVHRDFYDKQLLIGAART
                     TLIDADTLALSDPALDVGNFLAHLTLRAKQNPAFHVFVKNGRRAFTAEYRVKDLSLPA
                     EEFWRSVRWWEAAALLRLGCLYSLRPRWKNLAIPLLEQARKMLTHAGGIDG"
     gene            979..1527
                     /locus_tag="AC16GM_04595"
     CDS             979..1527
                     /locus_tag="AC16GM_04595"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR2922991.1"
                     /translation="MGRTICLLLFTLLFLGGPAAAPPPPRPQYLKLKVGDTVEVKGNW
                     DRKQQIFVASEIEKLPAPRRPKLRGPIGKILLADKSFVLFGKKISTDTRTEFLDSGSQ
                     KVHFADLKASQRVEVTCNPDEKGNWTARKIAVEKVKPTDKIKATVTRLAFTGTPPDTF
                     EVSGLKILVGAGTEMFGPWPEK"
     gene            1546..3351
                     /locus_tag="AC16GM_04600"
     CDS             1546..3351
                     /locus_tag="AC16GM_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024765.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HIR2922992.1"
                     /translation="MTTKRIATFWTAAFFAAALVPRITWAGSFTKLKAGDGVEVSGTW
                     DPKGFFAAKSVEKLKEPRRPTLRGAIEKVGKSDSSFVLFGRKIHVNGKSAFWAKGKPV
                     GDFGRLKAGLRVEVTCNATEAGVWEGRKIEWDGVKPSDKIKGTLTRVAFHNQPPDTLE
                     IYGLKILVNNNTKLSGSTRYIEEELFSTLSPDEAEAYLPRLKLGEQLLISGDYIQSTR
                     FENRYTLSQVQRDDYQATEPGLRVGAAGDWTPEVQSFLQLRLRKKFTFGTFPNRPPSA
                     ESTRVEFQAIQAYLLFRNPGGQGVALAVGKQRVRDERRWLFYEYLDAARLYVYETHPI
                     VLEASYLPSLFPLRDKKFKTWDDILFQVRYLPDAKNEARLYFLKRRDSSPRQREPVYW
                     GLSYYGRPRRFVTGWLEASVLRGTDKQRTQQAFAVDVGGTLAAVKFKVRPSVTLGYAV
                     GSGDKVRGDAVSQEFRQTGYETNAGRFGGFAIFQYYGEVLNPELANIQILTVGAGLRP
                     HPLVSADAVFHTYRQNQLGTKIRLESQGLIAPVVVPSGVDKNLGWELDFVLGIEKIWQ
                     RLNLGYAFGLFHPGQGFSPRTDQAVLNRLNLRVAF"
     gene            3354..>4554
                     /locus_tag="AC16GM_04605"
     CDS             3354..>4554
                     /locus_tag="AC16GM_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR2922993.1"
                     /translation="MRAKVLWLFPLLAVFLGAGPGYSQCPLPCSQGFINVQGKVVDVN
                     SAPVHKVNLDFFPNSFGIKIATFCDKTNILGNYNTCVYPPDFYRITFNPLYPFNTHLV
                     GKETLNVDLHAPTVLRDILLEFGWVASGQATDSLGKPVDSVRLSVENLVTQRRVYLST
                     PGNRTDSLGNYRIVIPNGNYRFRYAPAAGRRQVGVQLDTVNNTAGRDTLFNVTIHSGL
                     FISGQILDSTFKPRLDSIPDVTFEIQDTLGKKIFLPRNKSDSAGKFTVVAPPGVFEFL
                     FVPPRDSHFVANRERVVRLADDTTFSQFLQRGVLLTVQVRDSLQRPVLFANLDLFRLF
                     PTPKKMFTPTDLADSAGVIKVAVPPDSYALVLKPPGGNTNFDTLFLPGLVKIFHDTTI
                     HLILPGRV"
BASE COUNT          950 a         1360 c         1277 g          967 t
ORIGIN      
        1 ccaagctcaa agtattgaaa aaatttttcc agccggagga ggctccggcg ctgctcaaca
       61 gtttgcaacc ggtcctgaac ggccggatca ccattgaaaa actcgacgtt ctgggctacc
      121 ggctggagct gcgcagccgc tcccttctgc gcctggactt aaaacaggat tcccgaacca
      181 cgacactcgt ggccaaagtg atgcggccgg aaagagcggc gcagctcttc tggcatctgc
      241 aggagctgga acgctccgga ttccataccc gggcggccga tgccattacc attccgcacc
      301 cgattgccta tgcgccggaa ggcattatct ggctggaagc cgtctttgat ccttcactcc
      361 atgatgtgat cggagaagaa gcgttcgttt ccggttgcgg ggccgccgcc cggaccttga
      421 ataagcttaa aaccgctccg ctggaaaacc tcgtccccta cactctggaa gaggagcttt
      481 tactgctccg gcaaaccggg gctgaaatcg ctcaaattta tccggttttg gcagagcgtt
      541 tgcaggaagt gctagcctta gtgctgcaaa acgctccggc agcccggcag aacagaccgg
      601 caccagtgca ccgcgatttt tacgacaagc agctgctcat tggtgcagcg aggacgacct
      661 taattgacgc ggatacgctg gccctgagcg atcccgctct ggacgtcggc aatttcctgg
      721 cgcacctcac cctccgcgcc aaacaaaatc ccgctttcca cgtattcgta aaaaacggtc
      781 gccgcgcttt caccgcggaa taccgggtga aggatctctc tttgcccgca gaggaattct
      841 ggcggagcgt tcggtggtgg gaagccgcgg cgcttttgcg cttgggttgc ctctattcct
      901 tacgtccccg atggaaaaat ctggcgattc cgcttctgga acaagctcga aagatgttga
      961 ctcacgccgg agggatcgat gggtagaacc atttgtcttt tgctgttcac tctcttgttt
     1021 ctgggcggac cggccgctgc gcccccgcct ccccggccgc agtacctgaa gctgaaagtc
     1081 ggcgatacgg tggaagtgaa ggggaactgg gaccggaagc agcagatatt tgtggccagc
     1141 gagattgaaa aactacccgc gccccgccgc cccaagctgc gcgggccgat cggcaaaatc
     1201 ctgctggcag acaaaagttt cgttctcttc ggaaagaaga taagcaccga caccagaacc
     1261 gaatttctgg acagcgggag ccagaaagtc cactttgcag acctgaaagc cagccagcgc
     1321 gtggaggtca cctgcaaccc ggatgaaaag ggaaactgga ctgcccggaa aatagcggtt
     1381 gagaaagtaa agcccaccga caaaatcaag gccaccgtca cccgtctggc ctttacggga
     1441 accccgccgg atacgttcga agtctccggg ctgaaaattc tggtcggggc aggaacggaa
     1501 atgttcggcc cctggccgga aaagtaagac agtagaaagg ggagaatgac gaccaaacgt
     1561 atcgccacct tctggacggc ggcttttttc gccgccgctc tggtgccacg gataacgtgg
     1621 gccggttctt ttaccaaact gaaagccggg gacggcgtgg aggtatccgg cacgtgggac
     1681 cccaaaggat tcttcgccgc caaatcggtc gaaaagctga aggaaccccg gcgcccgacg
     1741 ctgcgcgggg ccatcgagaa agtgggcaaa agcgactcca gctttgttct gtttggaagg
     1801 aaaatccacg taaacggaaa atctgctttt tgggccaagg gaaaaccggt cggggatttt
     1861 ggccggctga aagccggcct gcgggtggag gtcacctgca acgccactga agcgggcgtc
     1921 tgggaaggcc gcaagataga atgggacggt gtcaagccct ccgataagat caagggcacc
     1981 cttacccgtg tggccttcca caaccagccg ccggataccc tcgagattta tggcctgaaa
     2041 attctggtga acaacaatac caaattgtct ggctcgaccc gttatattga agaggagctt
     2101 ttcagcacgc tttcccctga tgaagccgaa gcctacctgc ctcgtttgaa gctgggggaa
     2161 cagctgctta tctccggtga ttacattcag tccacccgct ttgaaaatcg gtacactctg
     2221 tcgcaggtgc agagggacga ttaccaggca accgaaccgg gtttgcgggt gggcgccgcg
     2281 ggcgactgga cgccggaggt gcaaagtttt ctccagttgc gcctgcggaa aaagttcacc
     2341 ttcggcacct tccccaaccg tcctcccagc gccgagtcca cccgggttga atttcaagcc
     2401 attcaggcct acctcctgtt ccgcaacccc ggaggccagg gagtggctct ggcggtcgga
     2461 aaacagcggg tgcgcgatga aagaaggtgg ctcttttacg agtatctgga tgccgcccgg
     2521 ctctatgttt acgagaccca ccccatagtt ctggaggcct cctatttgcc ttccctcttt
     2581 cctttgcgcg acaaaaagtt caaaacctgg gacgacattc tctttcaggt tcgctacctg
     2641 ccggacgcca aaaatgaggc ccgcctctat ttcctcaagc gccgggactc atcaccccgc
     2701 cagcgggagc cggtctactg ggggctgtcg tactacggcc gccccaggcg ctttgtcacc
     2761 ggctggctgg aggcctcggt tttgcgcggc acggacaagc agcgcacaca acaagcgttt
     2821 gcggtcgatg tggggggcac cctggccgcc gtcaagttca aagtgcgtcc cagcgtcacc
     2881 ctgggatatg ccgttggctc gggggacaaa gtccggggcg atgcggtttc gcaggagttc
     2941 cgccagaccg gctacgagac caacgccggc cgcttcggcg gcttcgccat ttttcaatat
     3001 tacggcgagg tgctgaaccc ggagctggcc aacattcaga ttttgactgt cggcgccggc
     3061 ctgcgcccgc atcctttggt gtccgccgat gccgtgttcc acacctaccg tcagaaccaa
     3121 ctgggaacga aaattcgtct cgagagccag gggcttatag ccccggtggt ggttccctcc
     3181 ggggtagata agaacctggg gtgggaattg gattttgtct tagggataga gaaaatctgg
     3241 cagcgcctca atctcggcta cgcctttggc ctgtttcatc ccggccaggg gttttccccc
     3301 cgcaccgatc aggcggtctt gaaccggctc aatctccggg tggcatttta gcaatgcgcg
     3361 cgaaggtcct ttggctcttc cccctcctgg cggttttttt gggggcaggg ccgggttatt
     3421 cccagtgtcc gctcccctgt tctcagggct tcataaatgt ccagggtaag gtggtggacg
     3481 tcaacagcgc cccggtccac aaggtcaacc tggatttctt cccgaacagt ttcggcatta
     3541 aaattgccac gttttgtgat aaaaccaaca tcctgggtaa ttacaacacc tgcgtttatc
     3601 cgcctgattt ttaccgcata acctttaacc cgctctatcc tttcaatacc cacctggtgg
     3661 gcaaggagac cttaaacgtg gacctgcacg ccccgacggt tctgcgggac atcctgctcg
     3721 aattcggctg ggtcgcttcc ggtcaggcca cagacagcct ggggaaacca gtcgactcgg
     3781 tccggctgtc ggttgaaaat ctcgttaccc agcggcgggt ctacctgagc accccgggca
     3841 acaggaccga ttctctggga aattaccgga ttgtgatccc gaacggcaac taccgcttcc
     3901 gctacgctcc ggcggccggc cgccggcagg tgggcgtgca gctggacacc gtcaataaca
     3961 ccgccggccg ggacacgctt tttaacgtta ccatccactc cggtcttttt atttccgggc
     4021 agatcctgga ctcgacgttt aagccgcgcc tagactcgat tcccgacgtt acttttgaaa
     4081 tacaggacac gctgggcaaa aagatttttc tgccccgcaa caagagcgac tccgccggga
     4141 agtttacggt ggtggccccg ccgggagttt ttgagttcct cttcgttccg ccgcgcgatt
     4201 cccacttcgt cgcaaacaga gagcgcgtgg tcaggcttgc cgacgatacg accttctcgc
     4261 aattcttgca acgcggggtt cttttgaccg ttcaggtgcg cgattccctg cagaggccgg
     4321 tgcttttcgc caatttggac ctcttccgcc tgtttccgac tcccaaaaaa atgtttacgc
     4381 ccaccgatct tgccgattca gccggcgtca tcaaagtcgc cgtccccccg gacagctatg
     4441 ccttggtgct gaagccgccc ggggggaata cgaatttcga caccttattc ctccccggcc
     4501 tggtgaagat atttcacgac acaaccattc atctcattct cccgggccgg gtct
//
LOCUS       DBNGZD010000172         7577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_375204, whole genome shotgun sequence.
ACCESSION   DBNGZD010000172 DBNGZD010000000
VERSION     DBNGZD010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_375204"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="AC16GM_04610"
     CDS             <1..536
                     /locus_tag="AC16GM_04610"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR2922994.1"
                     /translation="FPFDTPAELGMIFDLCQKLEVDCATNEAFAKGGNGAKELAEKTV
                     ALADDHRDPEPTFLYDLTLSVEEKVEKIATQMYGAAGIYFEKRAKKKIEKFVELGYGN
                     LPVCIAKTQSSLSDNPRARGVPKGWTLTVSDAFLSAGAGYLVLACGEMMLMPGLPKIP
                     AAVQMDVDEHGNITGLF"
     gene            590..1177
                     /locus_tag="AC16GM_04615"
     CDS             590..1177
                     /locus_tag="AC16GM_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR2922995.1"
                     /translation="MTTARIETGFLGQAFILPRIAVNAAGIVFFAIATAIGAQIRIPL
                     PFTPVPVTGQTFFVLLSGLTLGSRLGGTTQLLYLLLGITGLPFFSSGKGGWSILAGPT
                     GGYLVGFVLAAWLAGYFPRPRKNLFTSIGALWFVSLSILSLGTLWLGVSLHLSPAAAI
                     QQGFLPFLVGDALKVLAASGLFRLYHRHAKKLFPR"
     gene            1199..1687
                     /locus_tag="AC16GM_04620"
     CDS             1199..1687
                     /locus_tag="AC16GM_04620"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR2922996.1"
                     /translation="MSSANLFTYGTLMDQSILAEQLGLSLEEVKQHFRFAKAELSGYR
                     RIFDVYRPQWDGLVLNLTPDPKATVVGILISGLSEKEPVQIDRIEGLSHERQEMTVRV
                     GNQLFLALVYVGRTSDTFHPIAEAYEKKILTIVDHLGGEIADNYYRLTLDPKGQPRYK
                     EK"
     gene            1994..3184
                     /locus_tag="AC16GM_04625"
     CDS             1994..3184
                     /locus_tag="AC16GM_04625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR2922997.1"
                     /translation="MEPKTVYLAGAVRTPIGKFGGALATLSAADVGTVAARAALSRAG
                     IKPEQVDEVIFGLARQAGCGPNPARQIGHRAGAPDTVPAFTVNKACGSGLKSIILAYQ
                     AILLGDAEIVLAGGMESMSNVPDFLPKARWGYRLGHAELVDGMYRDGFLCPLCGQVMG
                     ETAENLAEQYRISRDEQDRYTVGSQRRCEQARKSGRFKDEIVPVETTDGKGRTTKVEA
                     DEHPRDNVTLEDLAKLKPVFRPGGTVHAGNSSGITDGAAAVVVISEQQAKRLKVKPQA
                     KIIAYASAGVDPAVMGLGPVPAVKKLLEKTKLSLADIDLVELNEAFAAQVLACQRELG
                     FDLSKVNVNGGAIALGHPIGCSGTRIVVTLVHEMQKRQARRGLATLCISGGMGLALLV
                     ESVS"
     gene            3210..3554
                     /locus_tag="AC16GM_04630"
     CDS             3210..3554
                     /locus_tag="AC16GM_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2922998.1"
                     /translation="MAEWSDWYPLTYDSVQAESQDLPGVYQIALAEGELEYGSARSPI
                     IYIEATYRGTLRTQLLEEVEGSGSPCVFDHLSRQPLIYRELATAEPEQEEEAALAEFE
                     REFGTLPACNKQ"
     gene            3567..4385
                     /locus_tag="AC16GM_04635"
     CDS             3567..4385
                     /locus_tag="AC16GM_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR2922999.1"
                     /translation="MTDKHVVLTTSGPIGTLTLNRPEKLNAFIDTMREEIAQGLEELG
                     NNTDVRVVVVTGAGRGFCAGADINYLAQLKRENDARNFKGLLEAGRRAITTIRNMDKP
                     VIAAINGPAAGGGLCLALACDLRIASDQATFGATFVKIGLHPDWAGGYFLPRLAGTAK
                     ALELMLTGEVINAQEAERIGLVNRVVPHDRLAAETKDLALKLSSRPANAVREIKNTAH
                     LSLDLSLEEMLDREFEVQMQMFHTPDMAEGVNAFLEKRTPVFGSAAGHSGRKEK"
     gene            4385..5347
                     /locus_tag="AC16GM_04640"
     CDS             4385..5347
                     /locus_tag="AC16GM_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR2923000.1"
                     /translation="MEQTTCPVQPAHAHRPKTAQVVQVSHGTHYHHRPVEQKRLKLAL
                     WLIGLTMIGECVGGFLTNSLALISDAGHMFTHFFALGVSFFAIRLASKKTGRDKSFGL
                     YRVEVLAALFNGITLVAITAYIFIEGVTRILHPQPVAELAMLGIAFVGLLVNLGSAVI
                     LWGASREDLNVRSAFLHMLGDTASSVGIVAAAIIISFTNWYILDPIASILIGAVILVW
                     AGRLLWDSVYVLLEATPRKIKVDEVITGLSEVEGVKTIHDVHIWEITSKMYSLTAHVV
                     IDDLRVSESQRIADRLSRLADQRFDICHTNFQFETFPQSESMLQ"
     gene            5418..6140
                     /locus_tag="AC16GM_04645"
     CDS             5418..6140
                     /locus_tag="AC16GM_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020375866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HIR2923001.1"
                     /translation="MQWRPDMKKLESPAEMSPEYRDLLITLLTVQCDTEFASVQQLRP
                     WVDMAPSINDRWAVSRVMMEEMRHGWQICQLLKSLAAEAVVQDLMWRKMGEHKLDSFN
                     IPFDEWSDVVLFNFLVDRVGLFQLTEFKDCSFGPLHRAVDTMLEEERFHISTGKSALA
                     KLCEEAEGRQQAQKAIYKWYPRALDMFGRAGSRRNEMARQFGIKKRKNEDARRDYVNE
                     VTPLIAELGLELPPEELNRRIL"
     gene            6215..6934
                     /locus_tag="AC16GM_04650"
     CDS             6215..6934
                     /locus_tag="AC16GM_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HIR2923002.1"
                     /translation="MTATIEKIETVDQMSPEYRDCLVGLLLVQADAELASVQLIRPWL
                     DQAPTVDDRWLLARRVTDAVQHAASIYRILKPLGAEAQMEQVLARKIGEHALDAFNMP
                     LETWPDLVLFSFLIDRVGVYQLREFVNSSYAPLARAVPKMVEEKTIHMHYGQNLLTAL
                     CQTDSGKKQAQAALRKWYPRALDMFGSAESKRNQQYRQYGLKKKTNEEARQSYVADVG
                     TILREQAGLEVPPPEFDRRIL"
     gene            7007..>7577
                     /locus_tag="AC16GM_04655"
     CDS             7007..>7577
                     /locus_tag="AC16GM_04655"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR2923003.1"
                     /translation="MDYSLSELMIAAAAREIHDPEIVFVGMRLPLLAFLVAKATHAPH
                     AIGLYENGIIRQKPAPELLYTMGDPPNVLGATFCGQMIDVMGFLQQGKVDVGFIGAGE
                     VDRFGNLNSSYVRDGNRLTRLPGSGGACDIASLAKRVVVILPHEKRRLKEQVDYVTSP
                     GYGTGNGWRRKVGLPHGGTSVVITTKGILR"
BASE COUNT         1617 a         2212 c         2218 g         1530 t
ORIGIN      
        1 ggttcccatt cgatacgccg gctgagttgg gcatgatctt cgacctctgc cagaaactgg
       61 aagtggactg cgctacgaac gaagcgtttg ccaaaggcgg caacggcgcc aaagagttgg
      121 cggagaagac ggtcgcctta gccgatgacc atcgcgaccc ggaaccaact ttcctctacg
      181 accttaccct gtccgtcgaa gagaaggtgg agaaaatcgc cacccagatg tacggcgccg
      241 ccgggatcta tttcgaaaag cgcgccaaga agaaaatcga aaaattcgtc gaactgggct
      301 acggcaatct gccggtttgc atcgccaaaa cccagtcctc cctttcggac aatccccgcg
      361 cgcggggcgt gcccaagggt tggactttga ccgtgagcga cgcgtttctt tccgccggcg
      421 ccgggtatct cgttctggca tgcggcgaaa tgatgctcat gcccgggctg cccaaaattc
      481 cggccgcagt tcagatggat gtggacgaac acggcaacat caccggtctg ttttgaaccg
      541 gcatgatctt ccgagacaga atgcgtcgaa ccaaccaggg gtgacaagag tgacaactgc
      601 cagaatcgaa accggatttt taggacaggc ttttattctg ccccgcatcg cggtgaatgc
      661 cgccggtatc gttttctttg ccatcgcaac ggccattggc gcacagatac ggattccgct
      721 tccctttaca ccggtccccg tcaccggaca aacctttttc gttctgctct ctggcttaac
      781 cctggggagc cgcctcggcg gaacgacgca attgctttac ctccttctgg gaattaccgg
      841 gctgcccttt ttctccagtg gcaaaggcgg ctggagcatt ctggccggac cgacgggggg
      901 gtatctcgtc ggctttgtcc tagccgcctg gctggccggg tatttccccc gcccccggaa
      961 aaatcttttc acgagcatcg gagcactctg gttcgtttcc ctttcaattc tctctcttgg
     1021 cactttgtgg ttgggcgtat ccttgcactt gtcgcctgcc gccgcaattc agcagggttt
     1081 cttgcctttt ctcgttggtg acgcgctgaa agtccttgcc gcttccggtt tgtttcgcct
     1141 ttaccaccgc cacgctaaaa aacttttccc gcgctaagat gaaaagagct cagcccctgt
     1201 gagctccgcc aacctattca cttacggaac cttgatggat caatccatcc tggcggaaca
     1261 gctggggctg tcgttggagg aagtgaagca acacttccgc tttgccaaag cggaactttc
     1321 cggctaccgg cgcattttcg acgtttaccg accgcagtgg gacggtttgg tgctcaacct
     1381 cacccccgac ccgaaggcta ccgtcgtagg gattctcatc tctggtcttt cggaaaaaga
     1441 accggtacag attgaccgaa tcgaagggtt gtcccacgaa aggcaggaaa tgaccgtgcg
     1501 ggtgggcaat caactttttc ttgcgcttgt gtacgtgggg cggacatccg acactttcca
     1561 cccgatcgct gaagcctacg aaaagaaaat cctgacgatc gtcgaccacc tcgggggcga
     1621 aatcgccgac aattactacc gactgaccct cgaccccaaa ggccagccgc gctacaagga
     1681 gaaatagggc gacttttagc gcggcgaggg cgccgcgcct acgttggaac gtcaaactcg
     1741 cgtagggcga ccgcccttgg tcgcccacga gaatgcttga gataaccggg ctcgtcgccg
     1801 gaccggacac cgaccactca gcgacgatct ggagaccgtc ggtacaaact tttgccttcg
     1861 accggctcgg agacgagccc gactaccttg gacaaaattt cagtactttt ttcacggaac
     1921 cggagagacc gggggagtcc tgcccggcga aagactttag cctcccgcaa cttttttact
     1981 atcttccccc ctaatggaac cgaagacagt ttacctggcc ggggccgttc gcactcccat
     2041 tggcaaattt ggcggagcgc tggctacact cagtgcggcg gatgtgggaa cggtcgcggc
     2101 ccgcgccgca ctctcccgcg cggggatcaa acctgaacag gttgatgaag tgattttcgg
     2161 cctggcccgc caggccggct gtggaccaaa ccccgcccgc cagatcggcc accgagccgg
     2221 cgcgccggat accgttccgg cctttacggt caacaaagcc tgcggctccg ggttgaagtc
     2281 gatcatcctg gcctatcagg ccattctctt gggggatgcc gagattgtcc tggccggggg
     2341 catggaaagt atgtcgaacg tccccgactt tttgcccaaa gcacgctggg gctaccggct
     2401 ggggcatgcg gagttggtcg acggaatgta tcgggacggc ttcctctgcc ccctctgcgg
     2461 ccaggtgatg ggcgagaccg cggagaatct ggctgaacag taccggatca gccgggacga
     2521 gcaggaccgc tacaccgtcg gcagccagag gcgctgcgaa caggcccgca aaagcgggcg
     2581 gttcaaagac gaaatcgtgc cggttgagac gaccgacggc aaaggccgca ccacgaaggt
     2641 cgaagcggat gagcacccgc gggataatgt gacgctggag gatctcgcca aactaaagcc
     2701 ggtttttcgc cccggcggca ccgtccacgc cgggaattct tccgggatca ccgacggagc
     2761 ggcagcggtg gtggtcattt ctgagcaaca ggcaaaaaga cttaaggtca agccgcaggc
     2821 caaaatcatt gcctacgcct cggcgggcgt tgatccggcg gtgatgggcc tgggtccggt
     2881 gccggccgtc aaaaaacttt tggagaaaac caaactctcc ttagccgaca tcgatctcgt
     2941 cgaattgaat gaagcctttg ccgcccaggt tctggcctgc cagcgggaat taggatttga
     3001 cctgtcgaaa gtgaacgtca acggcggggc aatcgcctta ggacatccca tcggctgctc
     3061 cggtacccgc atagtcgtta ccctggtaca cgaaatgcaa aaacgccagg cccggcgcgg
     3121 gctggcgacg ctctgcattt ccggcgggat gggactggcg ctcttagtcg aaagcgtgtc
     3181 gtagtttcga aaaatagcag ggaggcgcga tggcggagtg gagcgactgg taccctttga
     3241 cctacgacag cgtccaggcc gagtcgcagg atttgcctgg agtttaccag atcgccctgg
     3301 cggaaggcga actggaatac gggtcggccc gcagccccat tatttacatc gaggcgacct
     3361 accggggcac cttgcggacg cagcttttag aagaggtgga aggcagcggc agcccctgcg
     3421 tctttgatca cctgagtcgc cagccgttaa tataccggga actggccacc gcggagccgg
     3481 agcaggagga agaagcggcg ctggcagaat tcgagaggga attcggcacc ctgccggcct
     3541 gcaataaaca gtgaggcccg caagctatga ccgataaaca cgtcgtcctg accacctcgg
     3601 gtcccatcgg cactctgacc ctcaaccgcc cggaaaaact gaatgccttc atcgacacga
     3661 tgcgcgagga aatcgcgcag gggctggagg agttgggcaa taatactgac gtgcgggtgg
     3721 tagtggtgac cggcgcaggg cggggtttct gcgcgggagc cgacatcaac tacctggctc
     3781 agttgaaaag agaaaatgac gcgaggaatt ttaagggctt gctggaagcg ggacggcggg
     3841 ccataacgac gatccgcaac atggataaac cggtgattgc cgcgatcaac ggcccggccg
     3901 ccggcggcgg tttgtgtctg gctctggcct gcgacctgag aattgcctcg gatcaggcga
     3961 cctttggagc gacctttgtg aagatcggcc tgcaccccga ctgggctggg ggttacttct
     4021 tgccgcgcct ggccggcacc gccaaagcgt tggaattaat gctcaccggc gaggtgatca
     4081 atgcgcagga ggccgaacgg attggtctgg tgaaccgggt cgtcccacac gaccggctgg
     4141 cagcggaaac caaagatttg gctctgaaac tctccagtcg tccggcgaac gcagttcggg
     4201 aaattaaaaa taccgcccat ctgagtctcg atctctccct ggaagagatg ttagaccgtg
     4261 aatttgaagt ccagatgcaa atgtttcaca ctccggacat ggccgaaggc gtgaacgcat
     4321 ttctggaaaa acggaccccg gtgttcggtt cggccgccgg ccattcaggc cgaaaagaaa
     4381 agtaatggaa cagacaacct gtccggtaca gcccgcacac gcccaccggc ccaaaacggc
     4441 tcaggtggtg caggtgagcc atggcacgca ctaccatcat cgaccggtgg agcagaagcg
     4501 gttgaaactg gccctctggc tgatcggatt gactatgatc ggcgagtgcg tgggcgggtt
     4561 tttgaccaat agtctcgcct tgatctccga cgccggccac atgttcaccc acttcttcgc
     4621 tttgggagtg agtttcttcg ccatccggct ggcctcgaag aaaaccggcc gggataagag
     4681 cttcggcctc taccgggtcg aagttttggc ggcgcttttc aacggtatca cgctcgtggc
     4741 tatcaccgct tacatcttca tcgagggggt cacccgcata ctccaccccc agccggtagc
     4801 ggaactggcc atgctgggca tcgcttttgt gggcctctta gtgaatctgg ggtcagcggt
     4861 gatcctctgg ggtgccagcc gggaagattt aaacgtgcgt agcgcctttc tgcatatgct
     4921 cggtgacacc gcctcctcgg tgggaatcgt cgccgcggcg attatcattt ccttcaccaa
     4981 ctggtacatc ctcgatccga ttgccagcat cttaattggt gccgtgattt tagtctgggc
     5041 cgggcggctg ctctgggatt ccgtgtacgt cctcctggag gcgaccccgc gcaagatcaa
     5101 agtggacgag gtgattaccg gtctatccga agtggaaggc gtaaagacga tacacgacgt
     5161 gcacatctgg gaaattacct ccaaaatgta ctcactcacc gcgcacgtgg tgatcgacga
     5221 cctgcgcgtg tccgaaagcc agagaattgc cgaccggctg agccggctgg ccgaccagcg
     5281 cttcgacatc tgccacacca acttccagtt tgagactttc ccgcaatcgg agtcaatgct
     5341 tcagtaacgt ccccccttct gctgctttac ccgcaaatgc ttttgtcata tgttctgcct
     5401 cacggaagga ggactctatg cagtggcgcc cggatatgaa aaaattggaa tcgccggcgg
     5461 agatgtcgcc ggaataccgc gacctcttga tcacgctatt gaccgtgcag tgcgacaccg
     5521 aattcgcctc ggtccagcag ctgcgcccct gggtcgatat ggccccctcc atcaacgacc
     5581 gctgggccgt ctcgcgggtg atgatggaag agatgcgcca cggctggcaa atctgccagt
     5641 tgctcaaaag cctggctgct gaggcggtgg tgcaggattt gatgtggcgc aaaatggggg
     5701 agcataagct tgattctttc aacatcccgt ttgacgaatg gtccgacgtc gtgctgttca
     5761 atttcttagt cgatcgggtc gggctgttcc agttgaccga attcaaagat tgcagtttcg
     5821 ggccgctcca ccgcgccgtg gataccatgt tagaggaaga gagattccac atctccaccg
     5881 gcaagtccgc cctggccaaa ctgtgcgaag aggccgaagg ccgtcagcaa gcgcaaaagg
     5941 cgatttacaa atggtatccc cgcgcgctgg atatgttcgg ccgggccggg tcaaggcgca
     6001 atgaaatggc ccggcagttc gggatcaaga agcgcaagaa tgaagacgcc cggcgggact
     6061 acgtcaacga agttaccccg ctgatcgctg aattaggcct ggaactgccg cccgaagagc
     6121 taaaccggag aattctgtag acagcgggca aggagatcgg gttctgcctc cggtttgtgg
     6181 ccagatcatt ctctctccct gatccgaaaa atcaatgacc gctactatcg aaaaaatcga
     6241 aaccgttgac cagatgtcgc cggagtaccg ggactgcctg gtggggctgc ttttggtgca
     6301 ggccgatgcc gaactggcct ccgtacagtt gatccgcccc tggctcgacc aagcaccgac
     6361 ggtggacgac cgctggcttt tggcccggcg ggtcaccgac gccgtccagc acgccgcctc
     6421 gatctatcgc atcctcaaac cgttgggagc cgaagcccag atggaacagg tgctggcccg
     6481 caaaatcggc gaacatgcgc ttgacgcctt caatatgccg ctggagacct ggccggacct
     6541 tgtgctgttc tctttcctca ttgaccgggt gggagtgtat cagttgcgcg agttcgttaa
     6601 ctccagttat gcccctttag cccgcgccgt gcccaaaatg gtggaagaga aaacgattca
     6661 catgcactac ggtcaaaacc ttttgaccgc cctctgccag accgacagcg gcaaaaaaca
     6721 ggcgcaggcg gcgctccgga agtggtaccc ccgcgcgctg gatatgttcg gttccgccga
     6781 atcgaagcgc aaccagcagt accgccagta cggtttgaag aagaagacca acgaggaagc
     6841 gcggcagagc tacgtcgccg atgtcgggac aatcctccgc gagcaagccg ggctggaggt
     6901 gcccccgccg gagtttgacc ggcgcatcct ctgaaaaagt gtcgaggtgt cggcgtgtcg
     6961 aagttaaaat cctaaaatcg aaaaaaaagt tattccctct tgaacgatgg attactcctt
     7021 atccgaattg atgattgccg ccgccgcgcg ggagattcac gacccggaaa tcgttttcgt
     7081 cgggatgcgc ctgccgcttt tggcattcct ggtcgccaag gcgacccacg ctccccacgc
     7141 catcggcctc tacgagaacg gcatcatccg tcaaaaacca gcgccggaac tgctctacac
     7201 catgggcgac ccgcccaacg tgctgggcgc caccttctgc ggccagatga ttgacgtgat
     7261 gggttttctt cagcagggaa aagtcgatgt cggtttcatt ggcgcggggg aagtagaccg
     7321 cttcggcaat cttaattcat cttatgtgcg cgacggaaat cgtctgaccc gcctgcccgg
     7381 cagcggcggg gcctgcgaca ttgcgtcatt agctaaacgt gtagttgtca tcctgccgca
     7441 tgagaagagg cggctgaaag agcaggttga ctacgtcact tcccccggat acggcacggg
     7501 caacggctgg cgaagaaaag tcggactgcc gcacggcggg acatccgtcg ttatcactac
     7561 caaaggcatc ttacggt
//
LOCUS       DBNGZD010000173        12355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1695642, whole genome shotgun sequence.
ACCESSION   DBNGZD010000173 DBNGZD010000000
VERSION     DBNGZD010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1695642"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="AC16GM_04660"
     CDS             <1..1070
                     /locus_tag="AC16GM_04660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2923004.1"
                     /translation="LKVNPREIDAALDEHIQKLKSQYPTEEAYQAQLAREGLSESDLR
                     KRYRPEVRNQLLKQRLLQAKTAKIGISSEEIQKFFERYRDSLPAQPASVKLAHILFAA
                     EPGQRTIDSLRSRAESVLLQLKEGADFAQLAKKYSDDPSGQNGGELGTFGRGEMVPEF
                     EKAAFALKSGELSGIVETQFGYHIIQSEGTDGQKVKARHILIAVKPSERDLEKAQALA
                     DSVYRLILRGADFADMAKRFSADDDSKNKGGELGWYAVADLTPEFKQAIDSLVADPAK
                     GGTAGQVSRPTRSQFGFHLLKLLERREGKKLALPEDYDTIKDLARREKTNQWLANWIK
                     ELKKKTYIEIKPASVAPAKAG"
     gene            1260..2153
                     /locus_tag="AC16GM_04665"
     CDS             1260..2153
                     /locus_tag="AC16GM_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HIR2923005.1"
                     /translation="MRRSTVTPRRVIPYIILFCCLCAVSSATADIDFGRIEKIVGATG
                     QVQDSVLVIRFPRSDLKVTIDGEPMPTALGFVSWTAWKRMGNAATVMGDLVLLEREIN
                     PVISAPAEANIEVTALHNHFLGEEPRILFMHIHGMGDADTLARGIHSALSKTATPAPV
                     SGATSTPPPALDTKKIEEIIGHPGQASGGVFKITIGRPGVIADGIEVTSTMGLNSWAG
                     FVGTNERAHVAGDVAMTTGEVNRVIRALRSGGIDVVAVHNHMLDEQPRIIFLHYWGTG
                     PAEKLAQIVRAAFDQAKGPVK"
     gene            2190..3200
                     /locus_tag="AC16GM_04670"
     CDS             2190..3200
                     /locus_tag="AC16GM_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR2923006.1"
                     /translation="MKKFSAILLAAFFALGAGAQDTAPLRLVQAIPLPNVEGRSDHMA
                     FDVKGQRLFVAALGNNTVEVLDLRTGKHIHSISGLYEPQGVGYVPEFNKIFVANGGDG
                     TCQVFDGTSFQLVSSVKFAADADNVRYDASTQRVYVGYGAGALGIIDAKDNKLVGDIH
                     LAGHPESFQLEKSGPIIYVNVPTAQQVAVVDRNKQAVIATWPLADFRSNFPMTLNETD
                     QRLFVGTRHPARVVVLDTKTGRMDAALPCGEDTDDLFYDAARRCLYVSCGAGLINVFE
                     QSDADQYREIAKIPTAPGARTSFWVPELGRLFVAVPHRGNQKAAILVFEAHTLSANTG
                     KD"
     gene            3219..4475
                     /locus_tag="AC16GM_04675"
     CDS             3219..4475
                     /locus_tag="AC16GM_04675"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2923007.1"
                     /translation="MTEPAPPREATPTAGLRDWFGLHRNVVTVSVASFLFQTGEELWQ
                     QFRSLYLRALGADERIIGGFGTVKDALDGLYQYPGGWVADRFGRRRALLLFSGLAIAG
                     YAIYLVAPSWQVMFCGLPFVMAWASLANPSIFATIGDALPKERRAIGFTVQSILRRVP
                     SIIAPPIGGLLIDYFTARHGSQWGVIYGVRVGLDITIVIALFTFWILRRTYLELSAAP
                     ARFGRRLDSPSGVRQLFRTLHPSLKALLLSDIFIRTAEGMGDVFLVLFAHDVIGVSGT
                     AYGGLIAVAKLTSILVYIPVAKKSDRLGRKPFALLTFIFFALFPLSVALARSWPLFLV
                     AFIINGLREIGEPPRKALIVDLADPSARGRTVGLYYLIRSLAIAPAATIGGILYYSVA
                     PQAPFYLASAFGFVGVLLFGFRVRED"
     gene            4515..5732
                     /gene="hflX"
                     /locus_tag="AC16GM_04680"
     CDS             4515..5732
                     /gene="hflX"
                     /locus_tag="AC16GM_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR2923008.1"
                     /translation="MRRFYETNHRRVAERAILVALARVGSDSYRERFSSQQNGLPGAD
                     ADESLTELDSLARTAGATVVGQVVQHRPRIDPAYFIGSGKVAEIRDLCQQEGANLLIF
                     DDDLSPAQVRNLEDATEVRVIDRSVLILDIFAFRARTREAKTQVELAQLKYLFPRMTG
                     RWSHLERQVGGIGVRGPGEQQLEVDRRQIRKRIGTLEKLLEKIDNQREVQRHRRQQFF
                     RVALVGYTNAGKSTLFNHLTRAEVIVEDRLFSTLDSTSRLVKWPLGPKMILTDTVGFI
                     RKLPTHLVASFRSTLDQVCQADLLLHVVDLASPHFRHQIAEVEKVLVAIEAQKIKRFL
                     VFNKIDALPNGVPLADLAGLNGEQFFISAEARLGLATLVETIRVQALSHTPVYTNSAY
                     PDGDSADGETKNA"
     gene            5857..6237
                     /locus_tag="AC16GM_04685"
     CDS             5857..6237
                     /locus_tag="AC16GM_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2923009.1"
                     /translation="MPRVLTTRKVLIVEDNQQMSRLLSEMLEVFDVESLCAASGGAAL
                     KMLEEQQFGLVITDLRMPEMSGVELLRAVKEKHPEVPVVVISGFATKKAESEIVAADA
                     DGFLQKPFRMEDIGRLLNRILPVG"
     gene            complement(6296..7735)
                     /locus_tag="AC16GM_04690"
     CDS             complement(6296..7735)
                     /locus_tag="AC16GM_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR2923010.1"
                     /translation="MIGFFAWCLFPPSEIAVAKKEHPVPAKPNYGAIAGQVYHHRFGL
                     IPKQKVVAKNLTTGQTKTATTDRLGLFRVDELPVGEYEVTALYKTQWGKLCQSVRVGK
                     NSLSVVSLGLDSIRTNVPVSTYDWEGLIFSNFGAFGHLAGIVRDNESKEPVLPNFEIL
                     PIDDITYLRLFPTFTRQTGDYFAAWIPAGIYTVSLTLGGFISVKVLGLRIAPDSISIL
                     NKGMTTRVLHMEENPFVWKERIIAKKYYDPSKLEKAFFTEVPRPVKRGYGSIAGQVSY
                     RRLRFIDPQVVQTKNLSTGHMKTVTTDRLGLFRIDKLPVGEYQVTTVFQDRWGSSCQE
                     IRVRKDSLSVVNLWLDSILMDFPVTYAWDGPIFSLKNSFGHLAGKVDHEQSDLPMTAV
                     CVMVPYPYYIVNRAVSDSNGHYFLPWIPTGVYTVMPGASLTPTLWQNVRIAPDSISIL
                     NLKITLQRNPKSTHDWHEQIVGKDTYQQK"
     gene            7881..9209
                     /gene="hisS"
                     /locus_tag="AC16GM_04695"
     CDS             7881..9209
                     /gene="hisS"
                     /locus_tag="AC16GM_04695"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR2923011.1"
                     /translation="MATKKITPRIFKGTRDFLPEEMLPREKILEVMKNTFRKYGFDPL
                     ETPAFEFVEVLSGKYGEEGEKLIYKLAYKGGNELALRYDLTVPLARVVAMYPDLVLPF
                     KRYQIQPVWRADNPQLRQGRFREFYQCDVDTIGSASMLADAEIIAIIAEVLGRLGFQQ
                     FKIRINNRKILAGLVLFAGVDIKREAEVCRAIDKLGKIGLEGVKSELKEAGIAEKSIA
                     KILDFCSISGDNATVLQEAQKRLASIPVAQEGLTELQELFGHLSALAVAESNITLDLS
                     LARGLDYYTGPIFEAVVPDLPHLGSVAGGGRYDNLIGLFTGKDLPATGTTVGLDRIFT
                     ALQQLERLDKSKSATQVLVCLFDTETQKRALEITQNLRKAGLAAETYLAPDKLKKQLT
                     YADRKKIPIVVIAGPEEMKNNTVTIRNMRTSEQTNISGTKLVSYLQEHLQ"
     gene            9272..10192
                     /locus_tag="AC16GM_04700"
     CDS             9272..10192
                     /locus_tag="AC16GM_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923012.1"
                     /translation="MVTALFLPQAAAGGVKENPKVREYHFFTARSDSAILRAEKKAET
                     TLQTNPGDPAANLELCKILIATGDYDRADDYGEKAVKLEPKNSTCHLWLARAYGMRAE
                     HSNFLKAFFLVKKAKGQFERAAALDSANLAAQFDLFQYYVFAPGIVGGSTKKALAQRR
                     VIARNPNAGAYRFLTDAILHHDKEELDSAEYYYRRAIAADSTQRTPLLLTIAFMYIAD
                     KQFDRGMAALLEYDTTAHNPAAYTDLLAPGFYRNGFLEKTRKEFEDHRKEWKIPELAF
                     PILARMYEREGNRAKAKEYYETAPLKHGER"
     gene            complement(10195..11715)
                     /locus_tag="AC16GM_04705"
     CDS             complement(10195..11715)
                     /locus_tag="AC16GM_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2923013.1"
                     /translation="MSSRWVLSVFLITSLSISASAQPLIRGFTRELAIHNLPTGFALK
                     SFDVTGDSVSELLITSSGRFLIFDGKTFNTLFQDSTTPGVNSLSPADVNRDGVTDLVA
                     VSGDSEIVVWYGPDFLNRRIFPVPRGFSTFAVRNREDGQIEFAFGFPHSDSSCGIINP
                     SDCLIEFWGYVLRYTDTTFTFSDSFNVDISPGPYGEMTYIDVIGSGQRDLILVGYYSS
                     YRSAPPYGTYWDLLVERVHGLQLFSVLSFPIGYSCYLNPCNRCSSFLRPILETYATGN
                     IDVDSAIEFVAFVSPTLPCRPSWSFCAYDFSTATKQWERTDSLDIRALFTVDLNRSAP
                     QEILAYEIQAGKPGLVEYRTSDGATLGYSELPFTPSTLLMGTFGSPPAPKVLLAHGDS
                     VVVYGIDVATAVRELPEETALPRQATLFPPSPNPFNGETAITYSIPATGNVSVRIIDI
                     LGREVRTLVAAAQKAGRYQVTWDATDDQGRSVGSGIYFAVLRGGGSAAAQKIVLLK"
     gene            complement(11776..>12355)
                     /gene="lepB"
                     /locus_tag="AC16GM_04710"
     CDS             complement(11776..>12355)
                     /gene="lepB"
                     /locus_tag="AC16GM_04710"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR2923014.1"
                     /translation="RTFVVQAYRIPSQSMEDTLLAGDFVLVNKVAYHFGEPKPGQVIV
                     FKYPLNPDKSYVKRVVALSGQTVAIRNKVLFIDGKPAPSYPGVKHTDSKILPPDFSSR
                     DNFGPIQVPPDNLFVLGDNRDDSQDSRVWGFVPKANLIGKAMFVYWSWKPDPKAPKWE
                     SPYITPLFAIFFYNLTHFPSRMRWDRLGQVVR"
BASE COUNT         2841 a         3439 c         3439 g         2636 t
ORIGIN      
        1 cgctcaaggt caacccgcgc gaaattgacg cggccctgga cgagcacatc cagaagctca
       61 aaagccagta tcccaccgaa gaagcgtacc aggcgcaatt ggcgcgggaa gggctcagcg
      121 aaagcgattt gagaaagcgc taccgcccgg aagtgagaaa ccagctctta aaacagcgcc
      181 tgctgcaggc caaaacggcg aaaatcggca tctccagcga agaaattcaa aagtttttcg
      241 aacgttaccg cgacagtctg cccgcccagc cggcctcggt gaaattggcc cacatcctct
      301 tcgccgcaga gcccggccag agaaccatcg actcactccg cagccgggcc gagtcggttt
      361 tgctccagtt gaaggagggg gccgactttg cgcagttagc caaaaagtat tcagatgatc
      421 cctccggaca gaacggcggt gagttaggca cctttggccg gggcgaaatg gtgccggagt
      481 tcgaaaaagc cgcctttgcg ctcaaatccg gagagctttc cggcatcgtc gagacccagt
      541 tcgggtacca catcatccag tcggaaggga ccgacggcca gaaggtcaag gcccggcaca
      601 ttttgattgc cgttaagccg tcggagagag acctcgaaaa ggcgcaggcg ctggccgatt
      661 cggtctaccg cttgattctc cgcggcgccg attttgccga tatggccaag cgcttctccg
      721 ccgatgacga ctccaaaaac aaaggcgggg aactgggctg gtacgcggtg gccgatttga
      781 ccccggagtt caaacaggca attgactcgc ttgtcgcaga tcccgccaaa ggcgggaccg
      841 caggccaggt aagccggccc acccgctcgc agttcggctt ccatttgctc aaactccttg
      901 agcgccggga aggcaagaaa ctcgccctgc cggaggacta cgatacgatt aaagacctgg
      961 cccgccggga gaaaaccaac cagtggctgg cgaattggat taaggaactg aagaaaaaga
     1021 cctatataga aatcaaaccg gcctcggtcg ctccggcgaa agcggggtga ccaataactg
     1081 caagttgccc gatttaaatc aaaacctctt atttcctcac acattcacac ctttaggaaa
     1141 aactaaacgt ataattgcgt aactgaacag cgcttccttg aagctaactg ctggcgcatt
     1201 gcaaaatgga caatctaaat gctgcagctg gaaacttgtt ttgaaaagag gaagagcata
     1261 tgcgaagatc aacagtaacg ccgagaagag tgattcctta cattattttg ttctgctgtt
     1321 tatgcgcggt tagctccgct accgctgaca ttgactttgg ccgaatcgag aagattgtgg
     1381 gcgcgacggg gcaagtgcag gacagcgtgc ttgtcatacg ctttccgcgc agcgacctca
     1441 aggtgacgat tgacggcgag ccgatgccga cggccttagg cttcgtttcg tggacggcgt
     1501 ggaagcgcat gggcaatgcg gccactgtca tgggcgacct ggtgcttctg gagagggaga
     1561 tcaatcccgt catctccgcc ccggccgaag ccaatattga ggtgacggcg ctgcacaacc
     1621 attttctcgg ggaagagccg cgcatcctgt tcatgcacat tcacggcatg ggagatgctg
     1681 acacgctggc gcgcgggatc catagtgcgc tgagcaagac ggcaactccg gcaccagttt
     1741 ccggtgctac gtcaaccccc ccaccggctc tggacaccaa aaagatcgaa gagatcatcg
     1801 gccatccggg tcaagccagc ggcggcgtat tcaaaatcac aataggtcgc cccggcgtca
     1861 tcgcggacgg gattgaggtg acttccacga tggggttgaa cagctgggcg ggatttgtgg
     1921 gcacgaatga acgcgcgcac gtggccggtg acgtggccat gaccaccggt gaagtgaatc
     1981 gtgtcatccg cgcgctgcgc agcggcggca ttgacgtggt ggccgtgcat aaccacatgc
     2041 tggatgaaca gccgcgcatc atcttcctgc actactgggg aacaggaccc gccgaaaaac
     2101 tggcgcaaat cgttcgcgcg gcgtttgacc aggcgaaagg cccggtcaaa tgaggccgtt
     2161 gcctcgaagc tattaagggg aaagaagaaa tgaaaaaatt ctcagcaatt ctccttgctg
     2221 cattttttgc gctcggcgcg ggtgctcaag acaccgcccc gctgcgattg gtgcaggcta
     2281 tcccgcttcc aaatgttgag ggacggagtg accatatggc ttttgatgtg aaaggccagc
     2341 gactcttcgt cgccgcgctc ggcaataata cggtcgaagt tctcgacctg cgtaccggca
     2401 agcacattca cagcatcagc ggcctgtacg agccgcaggg cgtcggctac gttcctgagt
     2461 tcaataagat ttttgtggcc aacggcggcg acggcacctg tcaggtcttc gacggcactt
     2521 cattccaact cgtctcaagc gtgaaattcg ccgctgacgc cgacaatgtc cgttatgacg
     2581 caagcactca gcgcgtgtac gtcggctatg gagcgggggc gctcggcatc attgatgcga
     2641 aggacaataa actggtgggc gacatccacc ttgcgggcca tcccgaatct tttcagttgg
     2701 aaaagtcagg gcccatcatt tacgtgaacg tccccaccgc ccaacaggtg gcggtggttg
     2761 accggaacaa gcaggcagtg atcgccacct ggccgctcgc cgacttccgt tcaaatttcc
     2821 ccatgacgct gaatgaaacc gaccagcgtt tattcgtggg cacccgccac cctgcgcggg
     2881 tggtggtgct ggacacaaag acgggtcgaa tggatgctgc tctaccatgc ggagaagaca
     2941 ctgacgatct tttctacgat gccgcgcgcc gatgtctcta cgtttcctgc ggtgcggggc
     3001 tcatcaacgt ttttgagcag tcagatgccg atcagtaccg ggagatcgca aaaattccca
     3061 ccgcgccggg cgcgcgcact tcattttggg tgccggaact gggccggctt tttgtggcgg
     3121 ttccacaccg cggcaaccaa aaagcggcca tcctggtttt tgaggcgcat acgctctccg
     3181 caaacaccgg taaggattaa atagatcagc cgaattcctt gactgaacct gctccacccc
     3241 gggaagccac tcccaccgcc ggcctgcgcg actggttcgg cctgcaccgc aacgtcgtca
     3301 cggtgtctgt ggcgtcgttt ctctttcaaa ccggcgagga gttgtggcag cagttccgct
     3361 cgctctacct gcgggcgctg ggcgctgatg aacggatcat cggcggcttc ggcacggtca
     3421 aggatgcgct cgacggcctg taccagtacc ccggcggctg ggtggccgac cgctttggcc
     3481 ggcgacgcgc ccttttgctc ttcagcggtc ttgccatcgc cggctatgcc atttacctcg
     3541 ttgcaccctc gtggcaggtc atgttctgtg gtctgccctt tgtgatggcg tgggccagtc
     3601 tcgccaaccc ctcgattttc gccaccatcg gcgacgcgct gccgaaagag cggcgcgcca
     3661 tcggttttac cgtgcagagc atcctgcgcc gggtgccgag catcatcgcc ccgcccatcg
     3721 ggggcctgct gattgactac tttaccgccc ggcacggcag ccagtggggc gtgatttacg
     3781 gcgtgcgggt ggggctggac attaccattg tcatcgccct ctttacgttc tggatactgc
     3841 ggcgaaccta cctcgaattg tccgccgcgc cggcccggtt cggtcgaaga ctcgactctc
     3901 cgagcggggt gcgtcaactg ttccgcaccc tgcatccgtc gctcaaggct ctgcttttgt
     3961 ccgacatctt catccgcacg gcggaaggga tgggcgatgt ttttctcgtg ctctttgccc
     4021 acgatgtcat cggagtctca ggcaccgcat acggcggact catcgccgtg gcgaaattga
     4081 cctcgatttt ggtttacatt cccgttgcca agaaatccga ccggctgggg cgcaagccgt
     4141 ttgcgctttt gacgttcatc tttttcgcct tgttcccgct ctcggtcgcc ctcgcccgca
     4201 gctggccttt gtttttggtc gcgtttatca tcaacgggct gcgcgaaatc ggcgagccgc
     4261 cccgcaaggc gctgattgta gacctggccg accccagcgc tcgcgggcgc acggtgggac
     4321 tgtattacct gattcgcagt cttgctattg cccccgccgc gactatcggc ggcatcctct
     4381 actactccgt cgcgccgcag gcgccgttct acctcgcgag cgcctttggc tttgtcgggg
     4441 tattgctctt tgggttcagg gtgagagagg attgactacg ccaaaagaaa atttgaccgt
     4501 atactgtagt gaaagtgcgg cgcttctacg aaacgaacca cagacgggtg gctgagcggg
     4561 cgatcttggt cgccttggcg cgggtcggga gcgactcgta ccgagagcgt ttctcgagcc
     4621 agcaaaacgg cctacccggg gcagacgcgg acgagtcact gactgaattg gattccctcg
     4681 cccggacggc cggggccacg gtggtggggc aggtggtcca gcaccgccca agaatcgatc
     4741 cggcctactt catcggctcc ggcaaggtgg ccgaaatccg cgacctctgt cagcaggagg
     4801 gtgccaacct cctcatcttt gacgacgacc tctccccggc ccaggtgcgc aacttggaag
     4861 acgccaccga agtccgggtg atcgaccgga gcgtcctcat cttggacatc tttgccttcc
     4921 gcgcccgcac ccgcgaggcc aaaactcagg tggaattagc gcaactgaag tacctctttc
     4981 cccggatgac cggccgctgg tcgcacttag agcggcaggt gggtggcatt ggtgtgcgcg
     5041 gccccggcga acagcagttg gaagtcgacc ggcggcaaat ccgcaagcgc atcggcacgt
     5101 tagagaaact attggaaaaa atcgataacc agcgggaggt ccaacgccac cggcggcagc
     5161 agttcttccg ggtggcctta gtcggctaca ccaacgcggg gaaatcgacc ctcttcaacc
     5221 acctgacccg ggccgaagtc atcgtggaag accgcctctt ttccacgctg gactccacct
     5281 cccggctggt caagtggcct ctggggccga agatgatcct caccgacacc gtgggtttca
     5341 ttcgcaaact gcccacccac ttggtggcct ctttccgctc cactttagac caggtctgcc
     5401 aggcggattt gctcctccac gtggtcgatt tagccagccc ccatttccgg catcagatcg
     5461 ccgaggtgga aaaggttctg gtagcgattg aagcgcaaaa gattaagagg ttcctggtgt
     5521 tcaacaagat tgacgcgctc ccgaatggcg tccctctggc cgatttggcg gggttgaacg
     5581 gcgagcagtt cttcatctct gccgaagcgc ggctgggtct ggccactctg gtggaaacga
     5641 tccgtgtgca ggcgctaagt catactcccg tttacacaaa tagcgcctat cccgacggcg
     5701 attcggctga cggtgagacg aaaaacgcgt gatcaatagt tgatcagcgt ttcgctcaat
     5761 acgagtctca ccgactgcgt ccccactaca tttcagaagc atttctcgct aaaaaaagta
     5821 aattttgccc ctctatcttc cgacaattac tatgacatgc cgcgagtcct gaccacccgc
     5881 aaagtcttga tagttgagga taaccagcag atgtcccgtc ttctgtcgga aatgctggaa
     5941 gtcttcgacg tcgaaagtct ctgcgccgcc tcgggcggag ccgccctgaa aatgctggaa
     6001 gaacagcagt tcgggctggt gattaccgac ctgcgcatgc cggaaatgtc cggcgttgaa
     6061 ctcctgcgcg cggtcaagga gaaacacccc gaggtgccgg tggtggtgat ctccggtttt
     6121 gccaccaaga aagcggaaag cgagatcgtg gccgccgacg ccgacggttt tttacagaag
     6181 cccttccgca tggaagacat tggccggctt ttgaaccgca ttctgccggt ggggtagagc
     6241 gcccccagtt aaatcacaaa tagaacattg gtaatagcaa ttagccggag ctaaattact
     6301 tttgttgata agtgtccttc ccgacaattt gctcgtgcca atcatgggta cttttaggat
     6361 tacgttgtaa agtgattttc agattcagga tcgaaattga atcgggtgca atccggacat
     6421 tttgccaaag agtcggggtt agtgacgccc cgggcatcac ggtgtagaca ccggtgggaa
     6481 tccagggaag gaagtagtgc ccattgctgt ctgaaacggc ccggttgact atgtaatatg
     6541 ggtaagggac catgacacat acggcggtca taggcaaatc actctgctca tggtcaacct
     6601 ttccggcaag atggccgaat gaattcttca gtgagaaaat tggtccatcc caagcatacg
     6661 ttactgggaa atccattaga attgaatcga gccacagatt cacaaccgat agagaatctt
     6721 ttcttacccg tatctcctgg caggaacttc cccaacgatc ttggaacaca gttgtaactt
     6781 ggtattcacc gacaggcaat ttgtcaatgc gaaagagacc caggcggtca gtggtgacgg
     6841 ttttcatgtg accggtggag aggtttttgg tttgaactac ctgcgggtct atgaacctca
     6901 agcggcgata agacacctgg cccgcaattg aaccatatcc ccgtttgacc ggtcggggaa
     6961 cctctgtgaa aaaggccttt tctaacttgc tgggatcgta gtatttcttt gcaattatgc
     7021 gttccttcca aacgaagggg ttttcttcca tatgtaatac cctggttgtc atacccttgt
     7081 tcagaatgga aatcgaatct ggggcaattc taagtccaag taccttaact gagatgaatc
     7141 ctcccaaggt tagagacaca gtatagattc cagctggaat ccacgctgcg aagtaatctc
     7201 cggtttgtct ggtgaaagtc ggaaagagac gcaagtaggt tatgtcatcg atcggcagaa
     7261 tctcaaaatt gggtagtact ggttccttgg actcattgtc cctcacaata ccggctaaat
     7321 ggccaaatgc gccgaaattt gaaaaaatta acccttccca atcgtaggtg gaaacgggga
     7381 cattcgttct aatcgaatcc agccccaagc tcacaacaga aagagaattt tttcctaccc
     7441 gcacgctctg gcagagtttt ccccattgcg ttttgtatag tgcggtaact tcgtactctc
     7501 caacgggcag ttcgtctacg cggaagagac ccaggcggtc ggtggtggcc gttttggtct
     7561 ggccggtggt gagatttttt gcaacaacct tctgtttggg tattaacccg aagcgatggt
     7621 ggtagacctg accggcgatt gcgccgtaat ttggtttggc agggacaggg tgctctttct
     7681 ttgcaaccgc tatttcgctc ggaggaaata ggcaccaagc aaaaaaacca atcaccagtg
     7741 ccctcttgaa tgaaatgttt gctgaatgtg aaagcattct acaatctttg acgtaaagtc
     7801 acatatataa ctaggcaaga gtttaattcc aactaccgga agattgacag cccgtcaaac
     7861 ggctattatc gttcgcttgc atggccacaa agaaaatcac cccccgcatt ttcaaaggga
     7921 cgcgcgactt cctgccggaa gagatgctgc cgcgcgagaa gattctggag gtgatgaaaa
     7981 acactttccg caagtacggt ttcgatcctt tggaaactcc cgcgtttgag ttcgtcgagg
     8041 tcctctccgg caagtacggc gaagagggag agaaactgat ttacaaattg gcctacaagg
     8101 gcggcaatga actggccctg cgctacgact tgacagtccc tttagcccgc gtggtggcga
     8161 tgtaccccga tctcgtcctg cccttcaagc gctaccagat tcagccggtc tggcgcgccg
     8221 ataatcccca actgcgtcag ggccgcttcc gggagttcta ccagtgcgac gtagatacca
     8281 tcggctccgc ttcgatgttg gccgacgcgg agatcatcgc catcatcgcc gaggtgctgg
     8341 gccgactcgg tttccagcaa ttcaagatca ggatcaataa ccgaaaaatt ctcgcggggc
     8401 ttgtcctttt cgcgggagtt gacatcaaac gtgaagcgga agtctgccgg gctatcgata
     8461 aactgggaaa aatcggactc gagggcgtta aaagcgaact gaaggaagcg ggcatcgccg
     8521 aaaaatcaat cgctaaaatc ctggatttct gctccatatc cggagacaat gcaactgtac
     8581 tgcaggaagc gcagaaacgg ctggcctcga ttcccgtcgc gcaggaaggt ctgaccgaac
     8641 tgcaggaact tttcggccac ctcagcgccc tcgccgttgc tgagtcaaat atcacccttg
     8701 acctctcctt ggcccgcggc ctggactact acaccggccc gatttttgaa gcggtcgttc
     8761 ccgacctgcc gcacctgggc agcgtggccg gcggggggcg ctacgacaac ctgatcggcc
     8821 tcttcaccgg caaagacctc cccgccaccg gaacgacggt cgggctggac cgcattttca
     8881 ccgctctgca gcagttagaa cggctggata agagcaaatc ggcgacgcag gttctggttt
     8941 gtttgttcga taccgaaacc cagaagcgcg cgctggagat aacgcaaaac cttcggaaag
     9001 ccggtctggc cgccgaaacg tatcttgcgc cggataaact aaaaaaacag ttgacctacg
     9061 ccgaccggaa gaaaatcccc attgtggtca tcgccggacc ggaggaaatg aaaaataata
     9121 cagtgacgat ccgcaacatg cgtacctcgg agcagacgaa catttccggg acaaaactgg
     9181 tttcttactt acaggaacat ttgcaatagc agtccctatg cggcagcttt tttctcggaa
     9241 acaaaagtca aaaaatttcc tccggattgc ggtggtaacg gccctttttt tgccgcaagc
     9301 cgctgccggg ggagtcaaag agaatccaaa ggtgcgggag taccacttct tcaccgcccg
     9361 gagcgattcg gctatcctcc gtgctgaaaa aaaagcggag actacactgc aaaccaatcc
     9421 cggcgatcct gccgccaatc tggaactgtg caaaattctc atcgccaccg gtgactacga
     9481 ccgcgccgat gactacggcg agaaagcggt caaacttgag cccaaaaatt ctacctgcca
     9541 cttgtggctg gcgcgcgcgt acggcatgcg cgccgagcac tccaatttcc tcaaggcctt
     9601 ttttctggtg aaaaaagcca aaggccagtt cgagcgggcc gcggcgctcg attcggccaa
     9661 tctggcggcg caattcgatc tcttccagta ctacgtcttt gcgccgggca tcgtcggcgg
     9721 tagcacaaag aaagcgctgg cccagcgcag ggtcatcgcc cgtaatccca acgccggcgc
     9781 ttaccgtttt ttgaccgatg ccatcctgca ccatgataaa gaagagctcg attcggctga
     9841 gtattactac cgccgggcca ttgcggccga ctccacgcag cgcactccgc ttttgctcac
     9901 catcgccttc atgtacattg ccgataagca atttgaccgg gggatggccg cgctcttaga
     9961 atacgatacc accgcccaca acccggccgc gtataccgac cttttggcgc ccggtttcta
    10021 ccggaacggt tttttggaga aaacccgcaa agagttcgaa gaccaccgca aggagtggaa
    10081 aatccccgag ttggcttttc ccatcctcgc ccggatgtac gagcgggaag gcaaccgcgc
    10141 caaagccaaa gagtactacg aaactgcccc cctcaagcac ggggagaggt aaatctattt
    10201 caagaggaca attttttgtg ctgcggcact gcctccccct cgcagcacgg cgaagtaaat
    10261 cccgctcccg acagatcgcc cctggtcgtc ggttgcgtcc caggtgacct gataccgccc
    10321 ggctttttga gcggctgcca ccagcgtccg cacttcccgc ccgaggatgt caatgatgcg
    10381 gacggaaaca ttcccggttg cagggatgct gtaggttatt gctgtttctc cgttgaacgg
    10441 gttaggcgaa ggcgggaaga gagtggcctg gcggggtagc gcagtctctt caggcaattc
    10501 ccggactgcc gtcgccacat caatgccgta gaccacaacg gaatccccgt gcgcaaggag
    10561 gactttggga gccggtggcg aaccgaatgt acccatcagg agagtggaag gtgtaaatgg
    10621 gagttcggaa tatcccagcg tggccccatc tgatgttcgg tactcaacga gtccgggttt
    10681 gccggcttga atttcgtaag ccaaaatttc ttgcggcgca ctgcggttga gatcaaccgt
    10741 aaaaagggca cggatgtcga gcgaatccgt tctttcccat tgcttcgtcg cggtagagaa
    10801 atcgtaggca caaaaggacc aagatggacg acaaggaagg gtgggggaga caaaagccac
    10861 gaattctatt gcagaatcaa cgtctatatt ccccgtggca taagtctcta ggatgggtct
    10921 taggaacgac gagcatcggt tgcacggatt gaggtagcaa gaataaccta tcgggaagct
    10981 cagaacagag aaaagttgta gcccgtggac acgttcaact agtaaatccc aataggtgcc
    11041 gtaaggcggc gccgaacgat aggaggaata ataaccaact aagatgaggt cacgttgtcc
    11101 tgagccaatt acatcaatgt aggtcatctc tccgtaaggg ccgggactta tgtctacgtt
    11161 aaaggaatcg gaaaaggtga aagtcgtatc cgtatacctt aggacataac cccaaaattc
    11221 tataaggcaa tcagaagggt taataatccc acaagaggaa tcactgtgcg gaaacccaaa
    11281 agcaaattcg atttgtccat cttccctgtt tcgcactgca aatgtactga aaccgcgtgg
    11341 caccggaaag atccttcgat tcagaaaatc aggtccgtac caaaccacta tttcactatc
    11401 accgctgaca gcgacaagat cggtgactcc atcccggttc acatccgccg ggctgagact
    11461 gtttactcct ggagtagtgg aatcctgaaa cagcgtatta aaggttttgc catcgaaaat
    11521 cagaaaacgt ccgctacttg tgatgaggag ttccgaaacg ctgtctccag tgacgtcaaa
    11581 ggatttaagc gcgaagccgg tcggcagatt atggatcgcc aattcacggg taaagcctct
    11641 tatgagaggt tgtgcggaag cgcttattga gagcgaagta attagaaaga cacttaaaac
    11701 ccaccgtgag gacatattga ctcctccagt ggaacttaat ataacctaaa gattctattc
    11761 agacaaggga atcttttacc gcaccacctg gcccagccgg tcccagcgca tacgcgacgg
    11821 gaagtgggtc aggttgtaga agaagatggc gaaaagggga gtaatgtagg gggattccca
    11881 tttgggtgct ttggggtcgg gcttccacga ccagtagacg aacatcgctt tgccgatcag
    11941 gttggccttg ggcacaaacc cccagacacg ggagtcctgg ctgtcgtcgc ggttgtcgcc
    12001 cagaacgaag aggttatccg gcggcacctg tatcgggccg aagttgtccc ggctggaaaa
    12061 gtctggcgga aggatcttgc tgtcggtatg tttgacgcca ggataggagg gggcgggctt
    12121 gccgtcgatg aagagcacct tgtttcggat tgcgaccgtc tgccccgaca gggccaccac
    12181 ccgcttgacg taacttttgt cgggattgag cgggtacttg aagacgatca cctgacccgg
    12241 cttgggttcg ccgaagtggt aggccacttt gttgaccaag acgaaatcac cggccaaaag
    12301 ggtgtcctcc atcgattgcg aagggatgcg ataggcctga acgacgaaag tgcgc
//
LOCUS       DBNGZD010000174         2180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_98270, whole genome shotgun sequence.
ACCESSION   DBNGZD010000174 DBNGZD010000000
VERSION     DBNGZD010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_98270"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1896
                     /locus_tag="AC16GM_04715"
     CDS             184..1896
                     /locus_tag="AC16GM_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2923015.1"
                     /translation="MGISVFAAVAWVAGCGRKPDENQAVALVGNHKIPLKQVLDRYPP
                     GTPFASAEEELNQKKALVQGLVEQVLLAEAGHRAGYDTLPEIVQQFEQEKDRTLIDAL
                     YETEVRKPVNVTEKDVKDWYDHLTEERRVSLIQVATQATAESLYQEIKKGKDFATLAL
                     ERSLDRNSNVKGGDIGFVQWGTIAQPLQEQVFSQGVGEVSRPVAVQKFWYLVKVVEKR
                     PFPRPDYGSARASLRQQLVAQRETDRANVFLDQLRTESAIVVDTAVAHEANRKLRELE
                     HGESPTGPVLRTFLDLSKVPASLGERPLAWCSGETLSVLKAYQALQGIMPLSRPHLET
                     EAQIKEIAFQIFLPQLLQAQARARELDRSARYREMLADLKDNLVAGVMRQKLIDAVHF
                     TDAEVRASYESRKPQYTQPAEFHLREIQLADSAEAVSLKKQIRSPEDFARFASAKTLR
                     PGYREKGGDIGYVRRESAPLFFAVVEKLAVNQVSSPVKNPDGTYSLLLLVERFPQKIT
                     PFDSVAHGIADTLLAERSRKLLDDWVAERQKTTKVTIDEDLLKKTIDYKWYAKKKESP
                     PVAG"
     gene            1898..>2180
                     /locus_tag="AC16GM_04720"
     CDS             1898..>2180
                     /locus_tag="AC16GM_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923016.1"
                     /translation="MSPAPLKEKTARLFLLAGFIAALAAAAPASAGSVLDRIAAIVGN
                     QIILESELKTQLALYAAQTGISLADSARLASLRSDLLEQMVDDRLVLQAA"
BASE COUNT          445 a          644 c          659 g          432 t
ORIGIN      
        1 cggacgatcc ctttccccct ggaattttcc agcgccggac ggttcggttt tcgggtgggg
       61 gtgccggacg ggccggggcc cgcccggctg gcgcaggtga agtcactctt gctcgccctc
      121 taaagaacgt atactttgcc ttagatgagt actacccgaa agacaatctg gttgttagca
      181 tttttgggca tttctgtttt tgcggcggtg gcctgggtgg ccggttgcgg gcgcaagcca
      241 gacgagaatc aagccgtcgc tctggtcggg aaccacaaga ttccgctcaa gcaagtcctc
      301 gatcgctacc cgcccggcac tccttttgcc agtgccgaag aagaactgaa ccagaaaaaa
      361 gctctggtgc aggggctggt ggagcaggtg cttttggcgg aagccggcca tcgagccggg
      421 tatgatactc tcccggagat tgtccagcag ttcgagcagg aaaaagaccg caccctcatt
      481 gacgctctct acgaaaccga ggtgcgcaag ccggtcaacg tcacggaaaa ggacgttaag
      541 gactggtacg atcacctgac cgaggaacgg cgcgtgagct tgatccaggt agcgactcag
      601 gcgacggccg agagtctcta ccaggagatc aaaaaaggaa aagattttgc gactctggct
      661 ttggagcgct ccctggaccg caattccaac gtcaaaggcg gcgacatcgg ttttgtgcag
      721 tggggcacga ttgcgcaacc gctgcaggag caggtttttt ctcagggagt cggtgaggtg
      781 tcgcggccgg tggcggtgca gaagttctgg tacctggtca aagtggtcga gaaacggccg
      841 ttcccgcgcc ccgactacgg gagcgcccga gcctcgttgc gccagcagct ggtagctcaa
      901 cgcgagacgg atcgcgccaa tgtctttctc gatcagctgc gcacggagtc ggctattgtc
      961 gtggatacgg cggtggctca cgaggccaat agaaaactcc gcgaactgga gcacggtgaa
     1021 tcgcccaccg gaccggtact gcggaccttc ttggatcttt ccaaggtacc cgccagtctg
     1081 ggggagcgac ccctggcctg gtgttccggc gaaacgctca gcgtgctcaa ggcctatcag
     1141 gccttgcaag gtatcatgcc cctttcccga ccccacctcg aaaccgaagc gcaaatcaaa
     1201 gaaattgcgt tccagatttt cctgccgcag ctcctgcagg ctcaggcccg cgcccgggag
     1261 ttagatcgtt ccgcccgtta ccgggaaatg ctggccgacc tgaaggacaa cctggtcgcc
     1321 ggggtgatgc gccagaagtt gattgacgcc gtgcatttta ccgacgccga ggtgcgcgcc
     1381 tcctacgagt cgcggaagcc gcagtatacc cagccggccg agttccacct gcgggaaatt
     1441 cagctggccg actcggccga ggcggtatcg ctcaaaaaac agatccgctc gccggaggac
     1501 tttgcccggt tcgcctccgc gaagaccctg cggcccggct accgggaaaa gggtggcgac
     1561 atcggctacg tgcgccggga atccgccccc ctcttctttg ccgttgtgga aaaattagcg
     1621 gtgaatcagg tttcttcccc ggtcaaaaac ccggatggca cctattctct tttgctgctc
     1681 gtggagcgtt ttcctcaaaa aattacgccg tttgactcgg tggcccacgg tatcgccgat
     1741 accctgttag ccgagcgctc ccgcaaattg cttgacgact gggtggcgga acgccagaag
     1801 accaccaagg tgacgattga tgaagatctg ctcaaaaaga cgatcgacta caagtggtat
     1861 gcgaaaaaga aagagagtcc cccggtcgcc ggctaagatg tcccctgccc ctttgaagga
     1921 aaaaaccgcc cggctcttcc tattggccgg gtttatcgcc gctctggcgg ccgccgcacc
     1981 tgcgtcggct ggcagcgttc tcgaccggat cgccgccatt gttggtaacc agatcatttt
     2041 ggaatcggaa ctaaagacgc aattggcgct ctacgcggcg cagaccggca tcagtctcgc
     2101 cgacagcgcc agactggctt ccctgcgctc cgatctcttg gagcagatgg ttgacgaccg
     2161 gctggtgcta caggcggcgg
//
LOCUS       DBNGZD010000175         7238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1761966, whole genome shotgun sequence.
ACCESSION   DBNGZD010000175 DBNGZD010000000
VERSION     DBNGZD010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1761966"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..777
                     /locus_tag="AC16GM_04725"
     CDS             178..777
                     /locus_tag="AC16GM_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR2923017.1"
                     /translation="MDFRDPKNQKMILAILGLFFLGYVWYVRIYDKTAAALKVKRAEY
                     ERLMADLKAVELKAKSFEGLKTEYQDLLGRYQSIELLLPEEKQVPALLQQLNSAGAGL
                     QVRVVEVTPQGTSPAGFYNTAAFTLSLRGSYHAIGNFLAAVANFPFIVNVSQLALSQP
                     AVTQQAAAAKNPNETVSASFRILTYYVREEEKLKKLELN"
     gene            790..2343
                     /locus_tag="AC16GM_04730"
     CDS             790..2343
                     /locus_tag="AC16GM_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923018.1"
                     /translation="MRLSRMVPGVFAVVCSAAVALAGDVSITEISIHRVGESVDVAVH
                     GTGPLTVTPKMAGKSGTQPFRLILDFPRAVRAFKGQSFNSLPTATLSAIRTSQYAVTP
                     EKVVRVVLDFAARPNYQIEQRGSAIVIHLPATPDEPNFPVWSTATVGTAPAPVPTLAA
                     TPEPEKAVPPLTEPAATPLEPDTAALEQGETQPETSAAEGKENDTSAVASEEMAPADS
                     QPLEPDATTAATPETTAGQAAAAEPTLSPGETLTSMAPAPADTSAESQPVRDKLFASA
                     ESLPSPPAPGANHLTASASNSTAVAPQQPAHLLPSAASTPETTGKAVSQAEPPAPKKS
                     SPAPPVKTPAGSAPAAEPTPPEVSVTDLTEPQGPTPAEAEEEVTEEEINPDEVAVEED
                     STEENFAPIPGVMLQTSPTRTLVKYHLYGRDPFSPLVEKAVGLSGKHRGRLPDVEALR
                     LVGILRGWSGSRALFEDREGRGYILRDGDRVRNGHVVSVSDRKVVFQINEYGWTRTVA
                     LTLLSDENE"
     gene            2363..3688
                     /gene="pilQ"
                     /locus_tag="AC16GM_04735"
     CDS             2363..3688
                     /gene="pilQ"
                     /locus_tag="AC16GM_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR2923019.1"
                     /translation="MRQSKRLVVKLLPVVASALLVWAAVGRGQGDQDKIIKSLNFQNA
                     EMRSVFGMLADYAKQNIVIAPTVTGTVTLALSDITWRQAFDIILKTYNLAGVQEEGYI
                     RVLPLRDYLSEQTEIEKARAEQKALVAVQLRVIPVENASAKDMKAAVKAMLSDRGFVD
                     TDERTNSIIVRDIPDNIVKIEEFVKGLDKETKQVKISAQLVEIKSEFLQELGVRWFAR
                     DSFFVDAFRTNNIQGHYEFEQRADAKVTDPVDNFTVGAIDRAFNLEAAIRAFAKDNKA
                     KVLAHPEVTTLDNKMAKIQIGSKIPIKQFDASGNVVITFINVGTLLEVTPHVTSGTRI
                     LLALKPEQSTFRTEAIGPVIDTKNAETNVVVNNGQTVVIGGLTTSTEEKQTVGLPILK
                     DIPVLGYLFRYTKKRLDTSDLVIFVTPTMVTSETMAPETGMTEPVPSGN"
     gene            3972..4220
                     /locus_tag="AC16GM_04740"
     CDS             3972..4220
                     /locus_tag="AC16GM_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923020.1"
                     /translation="MRSRLCHRAHDRQERVFSHRGRGKADRWFDRTAAFSYSPSEDRL
                     RMLLFFANDVVSGIGVLTLRWEIAGPATKKPGGGYLRG"
     gene            4213..4341
                     /locus_tag="AC16GM_04745"
     CDS             4213..4341
                     /locus_tag="AC16GM_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923021.1"
                     /translation="MAESVLQLIRIPKVQIAAVAQELDLPHRKEMAVRDGTGQRSP"
     gene            4426..5115
                     /locus_tag="AC16GM_04750"
     CDS             4426..5115
                     /locus_tag="AC16GM_04750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2923022.1"
                     /translation="MSKQPLLTVVIPVYNEERTLSKVLERVFASPLDLQVIVVNDGST
                     DGTEEVLAHSPFPILHLKNPVNNGKGASIRKALEHARGEYVIVQDADLEYDPQDFQKL
                     LACAQRYQTPVVFGRRIRTWKEKIRHWKFFTAGKLLTWLANILYGCRVTDEPTCYKLV
                     RTDLLKALDLQAQRFEFCPEVTAKVAKRGIRIGEVPISYSPRDKKGGKKIKYRDFFEA
                     VWTLLRYRFRS"
     gene            5296..>7238
                     /locus_tag="AC16GM_04755"
     CDS             5296..>7238
                     /locus_tag="AC16GM_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923023.1"
                     /translation="MFSILFLVAFGLRFLFPAADPPWHLSWSQDLSGDPPQYTSGARN
                     HVLFGQWEPLGDLRFVTFSKSALTLFAYPVFLLFGVGRWQATLVAAFMSTLIVVLLAL
                     GLRRVFGWRAGFCAGSLLAVSTFATFYGRVTFLEIPLLLFLAGVFFCFCLSRDSLFIS
                     GCAGACCALSVFFGKPFGFVGVPALAGGWFNRHWSSPRKTARMFVRPLWGITTGFLVI
                     GLFWFFWVFLPNPNEVRSYYREQSLGLYGAPLGLESLSGFFLQVASLGNDTAYFSRQP
                     VLSLLGSAALVLLGWRLRSPRRPRSPAEKNQLSIYIFLAVWLIVGYLGLMPWNYRPLR
                     YQLLLTFPLCGISGILLWEWWQGAKVQFSRSPRWWFWFFLSGIGWLVNMHILYPRFPR
                     GYAPAQFSEMYVLALLMTAPYFLTALFFLRREKRPPLPPRTRTSWKARRILVLALVLA
                     IVGSDGKRYLAWAVNLPYSLSRASRELGELLEPRAVLSGPYGQVLAVENRLRVVTHNF
                     GLAFNPRFFAQFPITHLALDPSNFEMATKQYPEILADASIVSRFWVRGIPVQIVRVSD
                     APTTGGHERYRLSAFEQAQALFARGQLDSAQAAVALLRERFPDNSRIFWLQAEVLVRS
                     GRLPEAAAALEQAVRLAPDDYLL"
BASE COUNT         1415 a         2171 c         2181 g         1471 t
ORIGIN      
        1 ctggagtacg cccgggagca ggaggtggaa ggccacctgg cctacaactt ccagctctcc
       61 tgcgatctgg agctggtcca gagccagccg gtggaaccgg tggagccggc acccatttcg
      121 gcggtcaagc cgtaaggcgg gcgcacccag cgttcgggag taagggagag acgcgcaatg
      181 gacttccgcg atccaaaaaa ccagaagatg atcctggcga tcctggggct gttcttcctg
      241 gggtacgtct ggtacgtgcg catctacgac aagaccgcgg cggcgctcaa ggtaaagcgg
      301 gccgagtacg agcggctgat ggccgacctg aaagcggtgg aattgaaagc caaaagcttc
      361 gaagggctaa agaccgagta ccaggacctc ttgggtcgct accagtcgat tgaactcctt
      421 ttgccggaag agaagcaggt gcccgcgctg ctccagcagt taaactcggc cggcgcgggg
      481 ctccaggtgc gggtggtgga ggtgaccccg caggggacca gtcccgccgg gttctacaac
      541 accgcggcgt tcaccttgag cctgcgcggc agctaccacg ccatcggcaa cttcctggcc
      601 gcggtggcca atttcccgtt catcgtcaac gtgtcgcagc tcgcgctgtc ccagcccgcg
      661 gtcacccagc aggccgccgc agccaaaaac cccaatgaga ccgtgagcgc ttcttttcgg
      721 attttgacct actacgtccg cgaggaagag aaattgaaga aattggaact taactgatag
      781 gtgacggcga tgcgtctttc ccgaatggtt cccggagtgt tcgcggtcgt ttgttccgcg
      841 gcggtagcgt tggccggtga cgtctcgata accgaaattt ccatacaccg ggtgggggag
      901 tcggtggacg tggccgttca cggcaccggc ccgctcaccg tgaccccgaa aatggcgggc
      961 aagagcggca cgcagccgtt ccggctcatc ttagactttc cgcgggcggt ccgggcgttc
     1021 aaaggccaga gtttcaactc cttgcccacc gccacgcttt cggccatccg cacgagccag
     1081 tacgcggtga ccccggagaa ggtcgtccgc gttgttctgg attttgcggc ccggcccaat
     1141 taccagattg agcagcgcgg ctctgccatc gtcattcacc tgccggccac ccccgacgag
     1201 ccgaatttcc cggtatggtc caccgcgacc gtcgggacgg ctcccgcacc cgtacctact
     1261 ttggccgcga cgcctgaacc ggaaaaggca gtaccgcctt tgaccgaacc ggcagccaca
     1321 ccactggagc cggacaccgc agcgctggaa cagggtgaaa cgcagcccga aacctcggct
     1381 gcagagggaa aggaaaacga cacctccgca gttgcaagtg aggaaatggc accggcggat
     1441 agtcaacctt tagagccgga cgccactacg gcggcgacac cggaaacgac cgccggtcag
     1501 gcagcggcgg ctgaaccaac tctttcaccc ggagagaccc tcacgagcat ggccccggct
     1561 cccgcagata cttccgcgga gagtcagccc gtacgggaca aacttttcgc cagcgccgag
     1621 tcgctaccgt ctccaccggc gccaggcgcg aatcacctga cggcctcggc atcgaattcg
     1681 accgcggtgg caccccagca gcccgcgcac ctcttgccgt cggctgccag tacacccgaa
     1741 acgacgggga aagcggtgag ccaggcggag ccgccggcac ccaaaaagtc atcccccgct
     1801 ccgcccgtaa agactccggc cgggtcagcc ccggctgcgg agccgacgcc gccggaagtt
     1861 tcggtcactg acctgaccga gccgcaggga ccgactcccg ccgaggcgga agaggaagtg
     1921 actgaagaag agataaatcc cgatgaggtc gcggtcgaag aagactcgac ggaagagaat
     1981 tttgcgccga tacccggcgt catgctgcaa accagcccga cccggacctt ggtgaagtat
     2041 cacctctacg gccgggatcc cttcagcccg ctggtggaaa aagcagtggg gctcagcggc
     2101 aagcaccgcg gccgtctgcc cgatgtcgaa gcgctgcgcc tggtggggat tctccggggc
     2161 tggtcgggca gccgggcctt gtttgaggac cgggaagggc gcggctacat tctgcgcgac
     2221 ggcgaccgcg tgagaaacgg ccacgtcgtg tcggtgagtg atcggaaagt ggtctttcag
     2281 ataaacgaat atggctggac gcggaccgtc gcgttgacgc ttttgagtga cgaaaatgag
     2341 tgacgaaagg cgaggtggcg ctatgcggca atcgaaacgg ctggtggtca aactgctccc
     2401 ggtggtggcc tctgcccttt tggtctgggc ggcggtcggc cggggccagg gcgaccagga
     2461 taaaatcatc aaatcgctca acttccagaa tgcggaaatg cggtcggtct tcggcatgct
     2521 ggccgactat gccaagcaga acatcgtcat cgcccccacg gtgaccggca cggtgaccct
     2581 ggccctttcc gacatcacct ggcgccaggc cttcgacatc attctgaaga cttataactt
     2641 agccggggtc caggaagagg ggtacatccg ggttttgcct ttgcgcgact acctgtctga
     2701 acagaccgaa atcgaaaaag ctcgggcaga acagaaagcc ctggtggcgg tgcagctgcg
     2761 ggtgatcccg gtggagaacg cctcggccaa ggacatgaaa gcggcggtca aggccatgct
     2821 ctccgatcgg ggcttcgtgg acaccgacga gcgcaccaat tccatcatcg tgcgcgacat
     2881 accggacaac atcgtcaaaa tcgaagagtt cgtaaaaggc ctcgacaaag agaccaagca
     2941 ggtcaagatc tcggcccagc tggtggaaat taaaagcgaa tttctccagg aactgggggt
     3001 ccgctggttt gcgcgcgact cgttcttcgt cgacgcgttc cggaccaaca acatccaggg
     3061 ccactacgag tttgagcagc gggccgacgc gaaggtgacc gaccccgtgg acaacttcac
     3121 cgtaggcgcc atcgaccggg cgttcaacct cgaggcggcg atccgggctt ttgccaaaga
     3181 caacaaggcg aaagtcctgg cgcacccgga ggtgacgacc ttagacaaca agatggccaa
     3241 gattcagatc ggttccaaga ttccgatcaa gcagttcgac gcctccggca acgtggtgat
     3301 cactttcatc aacgtgggca ccctcctgga agtgacgccg cacgtcacct ccgggacgcg
     3361 cattttactg gcgctcaagc cggagcagag caccttccgc accgaggcaa tcggtccggt
     3421 gatcgacacc aagaacgccg agaccaacgt tgtggtgaac aacgggcaga cggtggtgat
     3481 cggcggcctc accacttcca ccgaagagaa gcagacggta ggactgccga ttctgaagga
     3541 catccccgtt ttagggtacc tgttccggta taccaagaag cgcctggaca cgagcgactt
     3601 ggtgatcttc gtaaccccca ccatggtgac gagcgagacg atggcgcccg aaaccggcat
     3661 gacggagccg gtaccgagcg gcaattagcc ggcgagagaa tggccgactg gggctcccca
     3721 ccgggagccc ttttcttttc tggaagcact tctctttcct gcgcttccca ctcgcttggt
     3781 atggggtttc gtctttcacc accagaggga aaacagcacc ggatcaggaa cacctccgtc
     3841 gatggttgcg gcagcaggct gaggggaaag aggaaaaggt cacgaacgcg atggtagccc
     3901 cctttggcgc cccacgctct ggcgattcct ttcgtcggcg gagggaggaa attcaaccac
     3961 gagcgcaccg ggtgcgatcc cgcttgtgcc accgagctca cgaccgtcag gagcgggtct
     4021 tctcgcaccg gggacggggg aaagccgacc gctggtttga cagaacggct gctttctctt
     4081 attctccttc tgaggatcgc ctgcggatgt tgcttttctt cgcgaacgat gtggtgagcg
     4141 ggataggagt gctgaccctg cggtgggaaa ttgcgggacc ggcgacgaag aagccgggag
     4201 gtggttacct ccgtggctga atccgttctc cagcttatca gaatacccaa ggtgcagatc
     4261 gctgcggtgg cgcaggagct cgatttgccc caccgcaaag aaatggcggt gcgggacgga
     4321 actggtcagc gatcaccctg aatcatcccg cgaggccgct ccgagcgggg ggcacgtgag
     4381 ccttttgggg ccgtccccgg aggaaattct ccccctgaga aactgatgag caaacaaccg
     4441 cttctgaccg ttgtgattcc ggtctacaat gaagagcgga ctctgagcaa agtattagag
     4501 agagtctttg catcgccgct cgacctacag gtgatagtcg tcaacgacgg ctccaccgac
     4561 ggcacggagg aggtgctcgc tcactcgcca ttcccgattc tccatttgaa gaatccggta
     4621 aacaatggca aaggggcgtc gattcggaaa gcgctggaac acgcccgcgg ggagtatgtg
     4681 atcgtgcagg atgcggattt agaatatgat cctcaagatt tccaaaaact cctggcctgt
     4741 gcccagaggt accagacgcc ggtggtgttt ggtcgtcgca tccgcacctg gaaggaaaaa
     4801 atacgccatt ggaaattctt tactgccggc aaactcctta cctggctcgc gaatatcctc
     4861 tacggctgcc gcgtcaccga tgaaccgacc tgctacaaac tggtccgcac cgacctcctc
     4921 aaggcgctcg acctgcaggc gcaacggttc gagttctgtc ccgaagtgac cgccaaggtg
     4981 gccaaacgag gaatccggat cggcgaagtg ccgatttcct actccccccg cgataaaaaa
     5041 gggggcaaga aaatcaaata ccgggatttc ttcgaagcgg tgtggacttt gctccgctac
     5101 cgtttccggt cctgagcgca tttcgtccgc agcgattctg ctcttctgtc acgtgccacg
     5161 acactctcgg ttggcgcgcg aatcacgagc atcgtatact gctgcgggat gggtgcagac
     5221 agaaaagagc cggccctgat atccgcgctc tctctgggcc aaagcggggt aaaagcgtgg
     5281 cggcgtgacc tctttttgtt tagtattctg tttttggttg ccttcgggtt gcgcttcctg
     5341 tttcccgcag cggatccgcc ttggcatctc tcctggagcc aggatctctc cggcgacccg
     5401 ccgcagtata ccagtggcgc gcgcaatcat gtcctcttcg gacagtggga gccgctcggg
     5461 gaccttcgtt ttgtcacctt ttcgaaatcg gcgttgacgc ttttcgccta tcctgtcttt
     5521 ttgctctttg gtgttgggcg gtggcaggcg actttggttg cagctttcat gagtacgctg
     5581 attgtcgtcc tgttagcact ggggttgcgt cgggtcttcg gttggagagc gggattctgt
     5641 gccggttcac ttttggcggt aagcacattt gctactttct acggacgggt gacgtttctt
     5701 gaaattcctc tcttactctt cctggcaggt gtcttcttct gcttctgcct cagccgggat
     5761 tcgcttttca tcagtggctg cgctggagca tgctgtgcgc tgagtgtttt ctttgggaaa
     5821 ccgttcgggt ttgtcggcgt gcccgccctt gccggcggat ggttcaatcg acactggtct
     5881 agtcctcgca agaccgctcg gatgttcgtt cgcccacttt ggggaatcac taccggcttt
     5941 ctggttatcg gactcttctg gtttttctgg gttttcctgc ccaacccgaa cgaggttcgc
     6001 tcctactacc gcgagcagtc cctggggttg tatggtgcac ccctgggact cgagtcccta
     6061 tccggctttt tcctgcaggt cgcttcgttg gggaatgaca ccgcctattt ctcccggcag
     6121 ccggttcttt ccctcttagg ctccgccgcc ctcgtactct tgggttggcg actgcgatca
     6181 cccagaaggc cgcggtcgcc tgccgaaaaa aatcagctgt caatctatat tttcctcgcg
     6241 gtctggctga tcgtcgggta cctgggactc atgccctgga actaccgccc gctgcgctac
     6301 cagttgctgc tgacatttcc cctgtgcggt ataagcggta ttctgctctg ggagtggtgg
     6361 cagggagcga aagttcaatt ttcccggtcg ccgcgctggt ggttctggtt ctttttatcg
     6421 gggatcgggt ggctggttaa catgcacatc ctctaccccc gctttccacg tggctacgca
     6481 ccagcacaat tttcggagat gtacgtcttg gcactcctga tgaccgctcc ctactttttg
     6541 actgcacttt tctttctgcg gcgagaaaag agaccccctc ttcctccccg aacccgcacg
     6601 tcgtggaaag cgcgacggat tctggtcctc gccctggtgc ttgcgattgt cggctccgat
     6661 gggaagcgct acctcgcttg ggcagtgaat cttccatact ccctttcccg agcgtcgcgg
     6721 gaactgggag aacttctcga gccccgggcg gtactctccg gaccgtacgg ccaggtgctc
     6781 gccgtggaga acaggttgcg ggtcgtgacg cacaacttcg gcttggcgtt caatccgcgt
     6841 ttctttgcgc agtttccgat cacccatctg gcgctggacc caagtaattt cgaaatggca
     6901 accaagcagt acccggaaat attggcggac gcttccatag tgagccgttt ttgggtccgg
     6961 ggaattccgg tgcagatcgt gcgggtaagc gatgccccga ccaccggtgg ccacgagcgg
     7021 tatcgactct cggcctttga acaggcccag gcgctttttg cccgcggaca actcgattcc
     7081 gcgcaagcgg ctgtggcact cctccgtgag cgctttcccg ataattcacg gattttctgg
     7141 ctgcaggcgg aggtgctcgt ccgcagtggc cgtctgccgg aagccgccgc ggcgctggaa
     7201 caggcggttc ggctggcccc ggacgattac cttctgat
//
LOCUS       DBNGZD010000176         4446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1626899, whole genome shotgun sequence.
ACCESSION   DBNGZD010000176 DBNGZD010000000
VERSION     DBNGZD010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1626899"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="AC16GM_04760"
     CDS             complement(<1..327)
                     /locus_tag="AC16GM_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR2923024.1"
                     /translation="MRTPGQTKIKKILALGFTGLALALFIAARERLPASGQVARVVDG
                     DTIVLESGEHLRYIGIDSPEHDDTLFAEARTLNQKFVEGKTVRLEYDVTKRDGYGRLL
                     AYVWLDQ"
     gene            complement(350..1330)
                     /locus_tag="AC16GM_04765"
     CDS             complement(350..1330)
                     /locus_tag="AC16GM_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR2923025.1"
                     /translation="MRLQATLLVLLSAVFSLCISVFAQEAKVGHGSLTLDGLVQVWYD
                     YTPDRSQKNGDTFRLRRTEFKFSGIVKPDRIKYFLLLDPASVPSTLIQDAGVAYAFPF
                     GVEVHVGQEKAPVSEEGLRPTPQVDFVEKSILALTFGEQRNIGLQVWGQKKRGEVRLA
                     VINGTGRNQQDNNRNKDFVGRLVVKPVPEISLGASGYTGRSTIGDSLRFKKWSVGGEA
                     GLDWRNWRLRGEILRGHGGKDDKNQTQEGVSGYLRYTYQEKIQAAVRVERWDVNLHQG
                     GDRQDVVTLGAGYLFDSFHARVLANFLVKQEEQGHRISNNEMQVLLQEAF"
     gene            complement(1388..2854)
                     /locus_tag="AC16GM_04770"
     CDS             complement(1388..2854)
                     /locus_tag="AC16GM_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR2923026.1"
                     /translation="MQPITFLLVGISLVVGFGLGALITSLVGRAQTKNAREQSQNAME
                     IVKQQIQQKDDRFIDLRNLYETALKDRAKAETELQAAEKSLAEQKTLIEKMAGDLKDA
                     FAAISQEALKSNSEEFLKLANSRFETLVTDAKGDLGKRQEAIDGLVKPLQDTLTRFDG
                     EVRKIEDSRKEDYGSLKQHLELLAQQTHTLGTALRTPQVRGRWGEITLRRAVELAGMS
                     EHCDFVEQESVAAENGLLRPDMIVRLPGGREIVIDSKVPLQAFLDAAESKNDPERNAA
                     LLRHAAQVREHMKKLSTKAYWEQFEKTPDFTVMFIPGESFFGAALEVDKSLIEQGAQS
                     NVILATPTTLIALLRTVAYGWRQEQIAQNALQISALGSEIHERISKFIEHFQKVGNAV
                     EKATDAFNAAAGALETRVLVTARKLKELGATSAPEIPLVGQIERLPRSVAIPAESEMV
                     TRLQGRSRVKLSKPSSSTESDEQLMLPAEPDNQAGAAP"
     gene            complement(3049..3939)
                     /locus_tag="AC16GM_04775"
     CDS             complement(3049..3939)
                     /locus_tag="AC16GM_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR2923027.1"
                     /translation="MNSRWKNLFGWSKSKADTWERCKKQFFYEYAAQYEAEPLGAQAK
                     SLKKLTKIPFLKGNLIHEAIAAEIQRRRQGQAVNPERAKAYARTRITEVTAQPKRFLS
                     EAVNGLSVTAQLPKAEEEVIQEIETFFSSIWPRYEEWEVLYVDGEEDFSSFVYGTAKV
                     FAEPDLVTKNPDGICLVTDWKTGAEWEEPEESEELSAQILWVERRYKLLLPQITAEYV
                     YLRTGQVRETRRTLEQMEKFVTRFIRSTSEMLQAENESDLPPTPSLKNCLDCKFATIC
                     EEGRPFLPSEPAVETTLLNS"
     gene            complement(3936..>4446)
                     /locus_tag="AC16GM_04780"
     CDS             complement(3936..>4446)
                     /locus_tag="AC16GM_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HIR2923028.1"
                     /translation="SQERQRMEKILGNRWLQIMLRLFLGGVFIYASVDKIAHPDQFAR
                     AIYNYRILPGELVNLLAIWLPWLEIATGAFLIFGVWLRASALLLTVQIAMFFIAASSA
                     LARGLNFACGCFTTDPKAQSAGITHLFLDGGLLLIALVILFFARRPRAGTEPITVPAT
                     AAPEPVSGG"
BASE COUNT          870 a         1276 c         1265 g         1035 t
ORIGIN      
        1 ttgatcgagc cagacgtagg ccaaaaggcg cccgtaacca tcccgcttgg tcacgtcata
       61 ttccagccgg acggttttcc cttccacgaa tttttgattc aatgttcgtg cttcggcgaa
      121 gagtgtgtcg tcatgctccg gcgagtcgat gccgatgtag cggaggtgct cgcccgattc
      181 gaggacgatg gtgtcgccgt cgacgacccg cgcgacctgc cccgacgctg gcaggcgttc
      241 tcgggctgca atgaaaagag ccaacgccag cccggtgaag ccgagtgcca gaattttttt
      301 gatttttgtc tggcccggtg ttctcaagcc cgtgttctgt gttctggtat tagaacgctt
      361 cctgcagcag cacttgcatt tcattgttgc ttatccggtg accctgttct tcctgtttga
      421 ccaaaaagtt ggccaatacc ctggcgtgaa aactgtcgaa gaggtacccc gcgccgaggg
      481 tcaccacatc ctgccggtcg ccgccctggt ggaggttgac gtcccagcgt tcgacccgca
      541 ccgcggcctg aattttttcc tgataggtgt agcggaggta gccggaaacg ccttcctggg
      601 tttgattctt gtcgtccttg ccgccgtggc cgcggaggat ttcgccccgc agccgccagt
      661 tgcgccagtc aaggccggct tcgccgccca cgctccactt tttaaaacgc agggaatccc
      721 cgatggtcga gcgccccgtg tacccggatg cacccaggga aatttccggc acgggcttta
      781 cgacgaggcg cccgacgaaa tctttattgc gattattatc ctgctgattc cggccggtgc
      841 cgttgatgac ggccaaacgc acttctcccc gtttcttctg tccccagacc tgcaggccga
      901 tgttccgctg ctcgccgaag gtcagagcca gaatcgattt ctcgacgaaa tcaacctggg
      961 gtgtgggccg cagcccctct tcagagaccg gcgccttttc ctggccgacg tgcacctcca
     1021 cgccaaaggg gaaggcatag gccacgcccg cgtcctgaat cagagtggac ggcaccgaag
     1081 ctggatcaag gagcagaaaa tatttgatcc ggtcgggctt gacgatgcct gagaatttga
     1141 attcggtacg gcgcaggcgg aaggtgtcgc cgttcttttg cgaccggtcg ggggtgtagt
     1201 cgtaccaaac ctggacgagc ccatcgaggg tcaaactgcc gtggccgacc ttcgcctcct
     1261 gggcgaaaac ggaaatgcag agggaaaaaa ctgcactgag caggacgagc aatgtggctt
     1321 gcaggcgcat gggcgagtcc tttcgttcgg gatgatgcaa atggaatgtc aggttagaaa
     1381 gttttcgcta cggcgcggcg cccgcctggt tgtccggttc ggcagggagc attagctgtt
     1441 cgtccgactc cgtagatgaa ctcggtttgg aaagtttgac tcgtgaacga ccttgtagcc
     1501 ttgtcaccat ttcgctttcg gcgggtatgg ccaccgagcg gggcaatcgt tcgatctgcc
     1561 ccaccagggg aatttcgggt gcgctggtgg cacccagttc tttcaatttt cgcgccgtga
     1621 ccaaaactcg cgtttccaaa gcccccgcgg ctgcgttgaa ggcgtcggtc gccttctcga
     1681 ccgcattacc cactttttgg aaatgttcga taaacttcga aatgcgctcg tgtatttcgc
     1741 tccccagagc gctgatctgg agcgcattct gagctatttg ctcctggcgc cagccgtagg
     1801 ccacggtgcg caagagcgcg atgagcgttg tgggcgtcgc caggatgacg ttgctctgcg
     1861 cgccctgttc gatgagcgat ttatccactt ccaaggcggc gccaaagaac gactcgcccg
     1921 ggatgaacat gacggtgaag tccggcgttt tctcgaattg ctcccagtag gctttggtgc
     1981 tcagtttctt catgtgctcg cgcacctgcg cggcgtggcg gagcagggcg gcatttcgct
     2041 ccgggtcgtt tttggactcc gccgcatcca gaaacgcctg caacggcacc ttggaatcaa
     2101 tgacaatttc ccgcccgccg ggaaggcgaa cgatcatatc gggccgcagg aggccgttct
     2161 cggcagccac cgattcctgc tcgacgaaat cgcagtgctc ggacatcccc gccagttcca
     2221 ccgcgcggcg gagagtgatt tccccccagc ggccccgcac ttggggagtc cgtagtgccg
     2281 tgcccaaagt gtgcgtctgt tgagccagta gctcaagatg ttgcttgaga cttccgtagt
     2341 cttctttacg gctgtcttct atcttgcgaa cttctccatc gaagcgagtc aaagtgtctt
     2401 gcaagggctt gaccagccca tcgatggcct cctgcctttt gcccaaatcg cccttggcgt
     2461 cggtgaccag ggtctcaaag cgcgagtttg ccaatttgag aaattcttca ctgttgcttt
     2521 tgagtgcttc ctgagagatg gcggcaaaag catctttaag gtctcccgcc attttctcaa
     2581 tcaatgtttt ctgttctgcg agacttttct ctgctgcttg gagttcggtt tccgccttgg
     2641 ctcgatcttt cagcgccgtt tcatacaaat tgcggaggtc aataaacctg tcgtccttct
     2701 gctgaatttg ctgcttgacg atctccatgg cgttctggga ttgctccctg gcattcttgg
     2761 tttgggctct gcctacgagc gaggtgatca gagctcccag accaaaacca accaccaaac
     2821 taatgccaac caacagaaat gttatcggct gcataccaac cccgctttcc gcttactcgc
     2881 cctccgagtg cacgaacata gagagaagat acatactccc tcgatttctt tcaacctctg
     2941 agcagcgttg gggcgaccga gggcggtcgc cctacgcgta gctagtgccc tttcgcgtag
     3001 gcgcggcgcc ctcgccgcgc aggatcctgt gggtaagcga atcaaaaatt aagaattcaa
     3061 tagcgttgtc tccaccgcag gctcggaagg caagaacggg cgcccctcct cgcagatcgt
     3121 ggcgaattta caatccaagc agttcttcag actgggagtc ggcggcaaat ccgattcatt
     3181 ttccgcctgg agcatttcgc tcgtgctgcg gatgaagcgg gtgacgaact tctccatctg
     3241 ttccagtgta cgccgggtct cgcgcacttg ccccgtgcgc aggtagacgt actccgcggt
     3301 gatctgtggc aagagtaatt tgtagcgccg ctccacccag aggatctgcg cggagagttc
     3361 ttcgctttct tccggctctt cccactccgc gccggttttc cagtcggtca ccaggcagat
     3421 cccgtcaggg ttcttggtga ccagatccgg ttcggcaaat actttggcgg tgccgtagac
     3481 gaaggaagag aaatcctctt ctccatccac gtagagaact tcccactcct cgtaccgcgg
     3541 ccaaatggat gaaaaaaacg tttcgatttc ctggatgact tcttcttcgg ccttcggcag
     3601 ttgcgcggtg acagagagac cgttgactgc ttcggagaga aaccgtttcg gctgcgcggt
     3661 gacctcggtt attcgggtac gagcgtaggc cttggcgcgt tcgggattaa cggcctgccc
     3721 ctgtcggcgg cgctgaattt cggcggcaat cgcctcatga atcagatttc ccttcaggaa
     3781 gggaatcttg gtcagctttt taagtgattt ggcctgtgcg ccgagcggct ccgcctcata
     3841 ctgagcggcg tactcgtaga aaaactgttt tttgcaccgc tcccaggtgt cggctttgct
     3901 cttcgaccag ccgaagagat ttttccagcg ggagttcatc cgcccgagac cggttccggt
     3961 gcggcggttg cagggacagt gattggctcg gtgcccgccc gaggtcgacg ggcaaaaaat
     4021 aagatgacga gtgcaatcaa aaggaggccg ccatctaaaa agaggtgcgt gatgccggcg
     4081 gattgggctt tgggatcggt ggtgaagcag ccgcaggcga aattcaaccc gcgcgccaag
     4141 gccgaactgg cagcgatgaa gaacatggct atttgtaccg tgagcaaaag tgccgacgcc
     4201 cgcagccaaa cgccgaagat cagaaatgcg ccagtagcaa tctccagcca ggggagccag
     4261 attgccagga gattgaccaa ttcgccgggc agaattcggt aattgtagat agcgcgcgca
     4321 aattgatcgg gatgagcgat cttatccact gaagcgtaaa tgaacacgcc gcccagaaag
     4381 agccggagca tgatttgcag ccaccgattg ccgagaattt tttccatcct ttgtctttcc
     4441 tggcta
//
LOCUS       DBNGZD010000177         7841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_931980, whole genome shotgun sequence.
ACCESSION   DBNGZD010000177 DBNGZD010000000
VERSION     DBNGZD010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_931980"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="AC16GM_04785"
     CDS             <1..715
                     /locus_tag="AC16GM_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HIR2923029.1"
                     /translation="TVEDIMDAYLQGWKLGLKALAIYRDGSKRTQPLVTGLGEPTATV
                     AAPRRRRLPDERKAVTHKFSIAGHEGYITVGLYDDGVPGEIFITMAKEGSTVSGLMDS
                     FATAISLALQYGVPLKTLVEKFSHTRFEPAGFTNNPEIPLARSVMDYIFRWVGKKFLP
                     PEDQPKGQTSATTEENGGTKTATAHPVVKQTTLFAGEKTDTFENQTDAPACPDCGMIM
                     VRNGNCYKCLNCGETSGCS"
     gene            complement(882..1640)
                     /locus_tag="AC16GM_04790"
     CDS             complement(882..1640)
                     /locus_tag="AC16GM_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR2923030.1"
                     /translation="MRRIAISILISLLGLAPVVLAGTAPGGAESAAEGKERGRAGALA
                     LRGFFGGDAAADSSKKSDTLDPCGQPVPPNLFQKVSTTGERPAGSPNASPATVLRKAD
                     LPKVPGGPKGTIYFFLGSLCPCTDAHRNSIAELMSLLPKRRVKPVAIFPNRGESVELV
                     EHFFRGLGFKMAYVIDSTNRLVDKFRAKKTPEVFLVDTKGKVVYSGSIDDSVENLGQV
                     KHAYLKNAVMDLTDGRPVAVSRAEGSGCWIVRKK"
     gene            complement(1663..2733)
                     /locus_tag="AC16GM_04795"
     CDS             complement(1663..2733)
                     /locus_tag="AC16GM_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR2923031.1"
                     /translation="MKKSKAVWAVAAGLVVLGGGWLWLAGCGGSAKGGIDTKHMVYGS
                     PESKMSMTPASGPLFEGAALSIAPEVKPVPNTNEHHVRIDVTHSEIVVNDTVKFIGWV
                     FGGMLPGPVLHVRQGDKVYFTMTNRSNETVGISPPSPHSIDFHAAMVNPKDKYREVIP
                     GATIEFEWTANYPGVYMYHCGSPAILQHMIYGMIGMTIVEPKDGYPTKVDREYALFQS
                     ELYLTKLKSGMYVTDMVTAKKKEPMFVCFNGKPAQYVKNPLKARAGERVRLYVLNAGP
                     NGTSSFHVVGTLFDRAGMEGNPLNEMRGAATILLPSSGGAIVEFVVPEKGIYTFVDHE
                     FADVEAGAVGLIDATSEEVAVK"
     gene            complement(3334..4122)
                     /locus_tag="AC16GM_04800"
     CDS             complement(3334..4122)
                     /locus_tag="AC16GM_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2923032.1"
                     /translation="MKRATAHFTVITASAVILAGIGFSWARPDSTGRGSLTGEAGRGK
                     LVYERYCISCHGAEGDGQGEFAEWISPKPRDFRQGMFKWRSTPSGSLPADADLEKTLE
                     NGTYGTHMPAWYVLGHREHREVIAYLKTFSPRWTKESAQPALVLPPEPPNDAASVERG
                     HAVYVSTGCDRCHGDRGQGDGPSARGLKDDWGYPIVPYNLTMGRIKCGETAADIYRVL
                     FTGLSGTPMPSFEKSLTGDQAWDLAHYILSLGPRAQQHIAKKGP"
     gene            complement(4132..5451)
                     /locus_tag="AC16GM_04805"
     CDS             complement(4132..5451)
                     /locus_tag="AC16GM_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923033.1"
                     /translation="MGRSLFKLLTGVTVAALLFILPLTQVSSIPSFARKTGLRCTSCH
                     ESWPVLNDFGRLYRDKGYQLRLGQDDPTTTTPGYWPAAIRATPHYEFNTLTNQETDSG
                     KTDFKTGGFADIGMDLLTGGTLSENAAFQVVAAGFSPDESPTLESYWVYLPKLFFKSD
                     WFNLRLGKYELDLPLSGHRSINLTNPYLVYGYHPEPDDNAAAAFSLAENQRGVEIVGT
                     DRRSFTRYVLSVFNANESPGSRTVFDSPSGYAHVQKFWQTASRAAPEIALGVFGAYAN
                     YPTTSLTSAGEPIPGQGGNLQGSLRYGGEAQVWFGPLVAPLHLVLVYAHGKDKRALFT
                     GADQDGIWNGGFAQLIWVPPAERLHWGLFARYDLIRNSRQPLAALPKDLNDQDQFTLG
                     LKYTISYSNRTEYALHAEYSTDRIRKTAFDGSDIRAGTAFIGIDFAY"
     gene            complement(5648..6397)
                     /locus_tag="AC16GM_04810"
     CDS             complement(5648..6397)
                     /locus_tag="AC16GM_04810"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR2923034.1"
                     /translation="MKRILTFTLMAFSIAATAWGGYDAIQVENGGWITGKISYAGGPP
                     VAKKIEPTTDKAACGKHGPIFSEELTVSPDSGLQNAVVSIANITAGRSVFSMAPPVLE
                     QNGCTFRPHVLVVPVGKPLTVRNSDGLLHNIHTHSLKNPPVNIAQAAAVPEITLGPYT
                     VPELVKVGCDIHGWMSAWFWVTNNPYAVVTGANGSYKIADLPPGKYVVEVWHETLGKI
                     SSEVTVAAGKETRLNLTFPAARPQVQAKKSD"
     gene            6881..>7841
                     /locus_tag="AC16GM_04815"
     CDS             6881..>7841
                     /locus_tag="AC16GM_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HIR2923035.1"
                     /translation="MKKGQFWALGLTGFFILASLWSLFPTFRFWTLPVEKRTEQPELE
                     KKAMKLGLDLRGGMHIVMEVDKSKLNEAEKKDAVDRALEIIRNRIDRFGVTEPQIQKS
                     GQDRIVVELPGVQEVERAKNLLGQTAQLEFKLLETTENTKVLLDRIDGLLAAAEARGR
                     TPGKPSPADTGADIFGRPKTKTPAQDTAAKRAESATDIFGQKTKKDTTKDTTKTAEEP
                     TTRPFSSLLEGLQFGYFQVVVDDVPTVKQILARPDVQNLIPPDDEFLWGSKTEIAGAQ
                     EFTFFYLTKKQVELSGKYLVNAQQAYGGGLVRSPIVNFKLTREG"
BASE COUNT         1564 a         2453 c         2172 g         1652 t
ORIGIN      
        1 gacggtcgaa gacataatgg acgcctacct tcagggttgg aagctgggct taaaggcgct
       61 cgccatttac cgcgacggct ccaagcggac gcagccgctc gttacgggct tgggcgagcc
      121 aactgcaacc gtcgctgcgc cgcgccgccg ccgcctaccc gacgaacgca aggccgtcac
      181 ccacaagttc tccatcgccg ggcacgaggg gtacatcacc gtggggcttt acgatgacgg
      241 cgtcccgggc gaaatcttca tcaccatggc caaagagggc tcgaccgtct ccgggttgat
      301 ggattccttc gccactgcca tttcgctcgc cctccagtac ggcgttccgc tcaagacctt
      361 ggtggagaag ttctcccaca cccgcttcga gccggccggc ttcaccaaca atccggagat
      421 tccgttagcc cgttcggtga tggactacat cttccgctgg gtggggaaga agttcctccc
      481 gccggaagac caacccaaag gacaaacgtc ggccaccacc gaagaaaacg gcggcacgaa
      541 gaccgcaacc gctcatccgg tagtgaaaca gacgactctt ttcgccggag agaaaaccga
      601 caccttcgaa aaccagaccg acgctcccgc ctgccccgac tgtggaatga ttatggtccg
      661 caacggcaac tgctacaagt gcctgaactg cggcgaaacc tcggggtgca gttaattttt
      721 acggtttcac cctaaaccgt aggggctggg tcacccagcc caaattcccg tagcgcgggt
      781 tggttttgta acccgcgctt tgctttttag cggagctgac gatttcctcc gtgccctctc
      841 gaagagtcgt gcagactgtg tttccgtgtt ggaattttct tttatttctt ccttacaatc
      901 cagcacccgc ttccttcggc tcgggacacg gccaccggtc gcccgtcggt caaatccata
      961 acagcgtttt tgagataggc atgcttcacc tggcctaaat tctccaccga atcgtcaatc
     1021 gatccggagt agaccacttt ccccttggta tccaccagaa agacctctgg tgttttcttg
     1081 gccctgaact tgtccactaa gcggttggtc gaatcgatga cgtaggccat tttgaatccc
     1141 agccctctga agaaatgctc gaccaactcg accgactctc cgcggttggg gaaaatggcg
     1201 accggcttca ctctccgctt gggcaaaagc gacatcagtt cggcaatcga attccggtgc
     1261 gcgtcggtgc agggacagag cgaccccaaa aagaagtaga tcgtcccttt tggcccgccg
     1321 ggtactttgg ggaggtcggc cttgcgcaac accgtcgctg gggaagcgtt cggtgaacct
     1381 gcgggacgct cccctgtcgt gctgaccttc tggaacaaat tgggaggtac aggttgtccg
     1441 cacgggtcga gcgtgtcgct tttcttgctc gaatcggcgg ctgcgtcccc tccaaaaaac
     1501 ccgcgtaggg cgagcgcccc cgctcgccct cgttctttcc cttctgccgc cgattccgcc
     1561 ccgcctgggg cggtcccggc caatacgacc ggggccagcc ccaagaggga aatcagtatg
     1621 gaaatcgcaa tccgccgcac ggcaaatctc aaaaagagac gcctacttta ctgccacttc
     1681 ttcgctcgtg gcgtcaatca agcccaccgc gcctgcttcc acgtcggcga actcgtgatc
     1741 cacaaaggtg taaatcccct tttcgggaac gacgaattcc acaatcgccc cgcccgaaga
     1801 aggcaaaaga atcgttgccg ccccgcgcat ttcgttcaag ggattcccct ccatcccggc
     1861 ccggtcaaag agcgtgccca ccacgtggaa actggaggtg ccgttcggcc cggcgttcaa
     1921 aacgtacagc cgcacccgct ccccggctct ggccttcagg ggatttttga cgtactgcgc
     1981 cggcttgcca ttgaagcaga cgaacatcgg ttctttcttt ttcgctgtga ccatgtcggt
     2041 cacgtacatc cccgatttca gtttggtcag gtagagctcc gactgaaaga gcgcgtactc
     2101 gcggtcaact ttggtgggat agccgtcctt cggctcgacg atcgtcatcc cgatcatccc
     2161 gtaaatcatg tgctgcagga tcgccggcga cccgcagtgg tacatgtaga cgcccgggta
     2221 attggccgtc cactcaaact cgatggtggc gccgggaatc acctcccggt atttgtcctt
     2281 cggattgacc atcgccgcgt ggaagtcgat cgaatgcggc gaaggcggcg aaatgcccac
     2341 cgtctcgttg gagcggttgg tcatggtgaa gtagaccttg tccccctgcc gcacgtgcaa
     2401 aaccggcccg gggagcatcc cgccgaaaac ccagccgatg aatttgaccg tgtcgttcac
     2461 cacgatttcg gagtgggtga catcaattct gacgtggtgc tcgttggtgt tcggcaccgg
     2521 cttcacttcc ggcgcgatgg agagcgccgc gccttcgaag agcggtcccg acgccggcgt
     2581 catcgacatt ttggactccg gcgagccgta gaccatatgc ttggtgtcaa tcccgccttt
     2641 ggcgctgccg ccgcagccgg cgagccagag ccaacccccc cccagtacga ccaaccccgc
     2701 tgccactgcc cagactgcct tactcttttt cacttcctcc tcctttgttg ctcccgcctg
     2761 ttatcgaatc cgacgtgaaa tgattatacc attttcccac atccctgaat acccgaaact
     2821 gctcacctcc tctgttttcc atgtcgtgtg tctcttgcgg attcctgttt tgaacttttc
     2881 gcgcccgcat gtgaaatatt tcacattacc ggacattcgt ccacctcttt cccatccata
     2941 cgctacctat ttactcttgt atgctattat cggatactat aatccgtttg tcaagtgctt
     3001 tttcgccccg ggagcgccca ccctcttccc ttcccatagg tcaggcatct cgcgagagtc
     3061 gcgccgacct gcctgaccgc tcacgtggcc ccggaaaggc cgacttcaac ctgtggacgc
     3121 caactatttc cctatcaccc aaagagacaa tgagctccgg ctgcggactg atcgggcata
     3181 gcccaagtag ccgccgcagc cggctttacc gtagttggtg caggtggtcg cgttagcccc
     3241 ggctcaattt caatgaaaaa gcggcttggc tcaaagaagt cccgggatgt ttagactctt
     3301 cttcttgcgg caccctgctc ggtaaccccg gccctatggg cctttttttg cgatgtgctg
     3361 ctgcgcccgg gggccgagac tcaaaatgta gtgggcgaga tcccaggcct ggtctccggt
     3421 gagcgatttc tcgaaggagg gcatgggagt cccgctgagg ccggtgaaaa ggacgcggta
     3481 tatgtcggcc gccgtttcgc cgcacttgat gcggcccatc gtgaggttgt acggcacgat
     3541 cgggtagccc cagtcgtctt tcaaaccgcg ggccgacgga ccatcgccct ggccccggtc
     3601 gccgtggcag cggtcacaac cggtgctgac gtaaacggcg tgcccgcgct cgacggaggc
     3661 ggcatcgttg ggcggttcgg gaggcaggac gagggcgggc tgggccgact ccttcgtcca
     3721 gcggggcgag aaggttttga ggtaggcaat gacttcccgg tgctcccggt ggccgagcac
     3781 gtaccaggct ggcatgtgcg tgccgtaggt tccattctcg agagtttttt caagatccgc
     3841 atcggcgggc aaagatcccg agggcgtcga gcgccacttg aacatcccct ggcggaaatc
     3901 ccgcggcttg ggcgagatcc attcggcaaa ctcaccttgc ccgtcgcctt cggcaccgtg
     3961 acaggagatg cagtatctct cgtagaccag ttttccccgc ccggcctccc ccgtcaggct
     4021 gccccggccg gtgctatccg gccgggccca ggagaagcca atacccgcca gaatgacggc
     4081 gctggcggtt atgaccgtaa agtgagcagt cgctcttttc atctcgtcct cctaataggc
     4141 gaagtcaatg ccgatgaaag ccgtccccgc ccgaatatcg gatccatcga aggcggtctt
     4201 ccggatccgg tcggtggaat actccgcatg cagcgcatac tccgtgcgat tgctgtagct
     4261 gatcgtgtac ttgaggccga gcgtgaactg atcctggtca ttcaaatcct tcgggagcgc
     4321 tgccaggggt tgccgggaat tccggatcag atcgtagcgc gcaaagagcc cccagtgcag
     4381 ccgctcggct ggcggaaccc agatcagctg cgcaaagcct ccattccaga tgccgtcctg
     4441 atcggccccc gtgaaaaggg cgcgtttatc cttgccgtgg gcgtagacca agaccagatg
     4501 caaaggcgca accaaaggcc cgaaccagac ctgggcttcc cccccgtagc gcagcgaccc
     4561 ctgcaagttt cccccctggc cgggaatcgg ctccccggcc gacgtgaggg aggtggtggg
     4621 gtagttcgca taggcgccga agacccccag ggcaatttcc ggcgcagcgc gggaggccgt
     4681 ctgccagaat ttttgaacat gcgcatagcc cgaaggggaa tcaaagacag tgcggctccc
     4741 gggagactcg ttcgcgttga aaaccgacag gacataccgg gtaaaagaac ggcggtcggt
     4801 gcccacaatt tctacgccgc gttgattctc cgccaggctg aaagcggctg cggcgttgtc
     4861 gtccggctcc gggtgatacc cgtagacgag gtagggattc gtcaggttga ttgatcgatg
     4921 gcccgaaagc ggcaggtcga gttcgtactt tcccaaccgc aggttaaacc agtccgactt
     4981 gaagaacaat ttcggcagat agacccagta cgactccaaa gtcgggctct cgtcgggaga
     5041 aaagcccgcc gcgacgacct gaaaggcggc gttttcgctc aaagtgccgc cggtcaacag
     5101 gtccatccca atgtcggcga acccgcccgt tttgaaatcg gtttttcccg aatcggtttc
     5161 ctggttcgtc agggtgttga attcgtaatg cggggtcgcc cggatcgccg cgggccaata
     5221 gcccggcgtt gtcgtcgtcg gatcgtcctg gccgagccgc agctggtagc ctttgtcgcg
     5281 atagagccgg ccgaaatcgt tgagcaccgg ccaggattcg tggcagctgg tgcagcgcag
     5341 tccggtcttc cgcgcgaacg aaggaatgga agaaacctgg gtgagcggaa ggatgaaaag
     5401 cagtgctgcg acagtaacgc cggtgagaag tttgaacagc gaacggccca tgaagcacct
     5461 cctgtcttga gccctgggct tgggcaccgc cggcgacgtg atcatgtcgg gcggctgcgg
     5521 tgggcgaacc gaacgttaag cacaacggtt gcggctgcca caggcagagc tgccccgcct
     5581 gccagccgca ttgcttcagc gagacttttc atccgtgact cttttcgggc ggctaaggga
     5641 cgaaccgtta gtcacttttt ttcgcctgca cctggggccg ggctgccgga aaagtcagat
     5701 tcaaacgggt ttcctttccg gccgcgacgg tgacctcgct tgaaattttt cccaacgtct
     5761 cgtgccagac ttcgaccacg tattttcccg gcggcagatc ggctatttta tagctgccat
     5821 tcgccccggt taccacggca tagggattgt tggtcaccca gaaccaggcc gacatccacc
     5881 catggatatc gcagcccacc ttcaccagtt ccggtacggt gtaggggcct aaggtgatct
     5941 ccggcaccgc ggccgcctgg gcgatgttaa ccggcgggtt cttgagcgag tgggtgtgaa
     6001 tgttgtgtaa aagtccgtcg ctgttgcgca cggtcagagg tttgccgacc gggaccacca
     6061 gaacatgcgg gcggaaggtg catccatttt gctctaagac cggcggcgcc atactgaaaa
     6121 ccgaccggcc ggcggttatg ttggcaattg aaaccacggc gttctgcagg ccgctgtccg
     6181 gggagaccgt cagttcctca gagaaaatcg gcccgtgctt gccgcaggcc gccttatcgg
     6241 tcgtgggctc gatctttttg gccaccggcg ggccgccggc atagctgatt ttcccggtta
     6301 tccagccgcc attttccacc tgaatagcgt cgtagccgcc ccacgccgtt gcggcgatag
     6361 agaaggccat caaagtgaag gttaaaatcc gcttcatcct ttcctcctta attaagggtt
     6421 atttgaccga accgctgaac aaaaaataat ctcttagcta tttctcttgt tatttttttc
     6481 cacaaccaaa ccttcgcgta cacccttact ccttcctcac cgatacgcta cccgacaact
     6541 cttgtttgct attatcggat accattatcc gcttgtcaag tactttttac tccagaccct
     6601 ttttttatct cgtccctggg tcaggcatct cgctagagtc gtgccgacct gcctgaccgc
     6661 tgacgcggtc ccgaaaaggc cgccttcaac ctgtggacgc cgcctttctt tccctatcac
     6721 ccaccacctt ccacctactt gtggtgagtc ccgcccatac gggccgtatg gccaaaggcg
     6781 ggatcgaacc atcacctgac ttttgacttt tatccccgcc cgctatatat accccgttcc
     6841 cccggtgcgg ttttcgcaac ccggggttgg acactgaccg atgaaaaaag gacaattctg
     6901 ggccctgggc ctcaccggct ttttcattct cgcttccctc tggagtcttt ttcccacttt
     6961 ccgcttctgg accctccccg tcgagaagcg caccgagcag ccggagttgg aaaagaaagc
     7021 gatgaagctg ggcctggatc tccgcggcgg catgcacatc gtcatggagg tcgataagtc
     7081 caaattaaac gaggccgaaa aaaaggacgc cgttgaccgg gcgctggaga tcatccgcaa
     7141 ccggatcgac cgcttcggcg tcaccgagcc gcagattcaa aagtcgggcc aggaccggat
     7201 cgtggtcgaa ctccccgggg tgcaggaagt ggagcgggcc aagaaccttt tgggccagac
     7261 tgcgcaatta gaattcaaac ttttagaaac tactgaaaat acgaaagtcc tcctcgaccg
     7321 cattgacggc ctgttggcgg cggcagaggc cagaggcagg acccccggga agccttcccc
     7381 ggccgatacc ggcgccgaca tcttcggcag acccaaaacc aaaactccgg ctcaggatac
     7441 cgccgccaaa agagccgagt ctgcgaccga catcttcggt cagaaaacca aaaaggatac
     7501 gaccaaggac accaccaaaa ctgccgagga acccaccacc cgtccctttt cctcgctttt
     7561 ggaaggtctc cagttcggct acttccaggt ggtggttgac gacgttccca cggtcaaaca
     7621 aattttagcc cggcccgacg tgcagaacct gatccccccg gacgatgaat tcctctgggg
     7681 ctccaagacc gaaatcgccg gcgcgcagga gttcaccttc ttctacctga ccaaaaaaca
     7741 ggtcgaactc tccggcaagt atttagtcaa cgcccagcag gcctacggcg gcgggctcgt
     7801 ccgctccccg atcgtcaact tcaaattgac ccgcgagggc g
//
LOCUS       DBNGZD010000178         1403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_306777, whole genome shotgun sequence.
ACCESSION   DBNGZD010000178 DBNGZD010000000
VERSION     DBNGZD010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_306777"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..790)
                     /locus_tag="AC16GM_04820"
     CDS             complement(263..790)
                     /locus_tag="AC16GM_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923036.1"
                     /translation="MSVRHVTVTAAFAAVTLFALLLSGVAAPPQSATVRFGLNEVGSP
                     FPPPAGHDHSTHAKDHIVPRTVVIAQGGSVTFEIDEFHQPALYRPGTAPEDITVSDAT
                     LQDLSLPCVPGGVLPKFVINDPNGRILLGPPHSCAEQTWTTPPGTFDHPGRYLVICTT
                     LPHFRNGMYGWVIVQ"
     gene            complement(980..>1403)
                     /locus_tag="AC16GM_04825"
     CDS             complement(980..>1403)
                     /locus_tag="AC16GM_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl cyclase domain-containing protein"
                     /protein_id="HIR2923037.1"
                     /translation="DYYGYFYGDKIIRLTARVVRDIVYDLAPEGFVGHVGGDDFVFIL
                     PTERIEAVCSQIIATFDRMIPYRYAEADRQRGYIVTHNRRGETEEFPLMTISIAVIPN
                     VAETFSHSGQFSHMLADLKKYIKTLPGSNYRIERRKKY"
BASE COUNT          295 a          391 c          446 g          271 t
ORIGIN      
        1 gtgacggcga cgttcaccgc gtcgaaggcc gcgggcgcgt cggtcaggaa gagtttcaat
       61 gttccgaact gattcttggt gctttttgag cacccgaaac cgaatagaag caagcctcca
      121 caaaggcaga cgaaaagccg tttcatcgca tacctcccat catcattcag ggaatacgat
      181 actacttttc ccgaaaatag caagtatttt ctcagcacca gcccggaagg gaggtgcaaa
      241 gtccgggcca gggcggacat ccctactgca caatgaccca gccgtacatg ccgttccgga
      301 agtggggcaa ggtcgtgcag atgacgaggt agcgtcccgg gtggtcgaac gtccccggag
      361 gcgtggtcca ggtctgctcg gcgcaagaat gcggcggacc caaaagaatt ctgccgttgg
      421 ggtcgttgat cacaaacttc ggaagaacgc cccccggcac gcaggggagc gagaggtcct
      481 gcagggtggc gtcgctgacg gtgatatcct cgggtgccgt ccccggacgg tagagggccg
      541 gctggtggaa ctcgtcgatt tcaaaggtca cgctgccgcc ctgggcaatg accaccgtgc
      601 gcggcacgat gtgatccttg gcgtgcgtgg agtgatcgtg gcccgcgggc gggggaaagg
      661 gagaacccac ctcgttcaag ccaaagcgta cggtagcact ctggggcggc gccgctactc
      721 cgctcaaaag cagggcgaaa agcgtgaccg ccgcgaacgc ggctgtgacg gttacgtgtc
      781 tcactgacat gactcctcct ccttctcctc tggaacgtgc gaactttaga gatttatacg
      841 gaagccgtga caggccgtta gcggcatccc ggatgccggt caaaaaagat gataaggtat
      901 ttcaatgacc tgcctggggc gactgccaaa actacgcggg tgtcaccgag aaccattccc
      961 caaaaggttt ttctaagaat caatactttt tgcggcgctc gatccggtag ttcgaccccg
     1021 ggagggtttt gatgtacttc ttcaagtcgg ccagcatgtg ggagaactgt ccggagtggg
     1081 agaaggtctc ggccacgttg gggatgaccg caatcgaaat cgtcatcagg gggaactctt
     1141 cggtctcgcc ccggcggttg tgggtgacga tgtaccccct ttggcggtcg gcctcggcgt
     1201 agcggtaggg gatcatccgg tcgaaggtgg cgatgatctg gctgcagacg gcctcgattc
     1261 gctccgtcgg gaggatgaag acgaaatcgt cgccgcccac gtgccccacg aagccttccg
     1321 gcgccaggtc gtagacgatg tcgcgcacca cccgcgcggt gagccggatg atcttgtcgc
     1381 cgtagaagta accgtagtag tcg
//
LOCUS       DBNGZD010000179         4081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_445686, whole genome shotgun sequence.
ACCESSION   DBNGZD010000179 DBNGZD010000000
VERSION     DBNGZD010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_445686"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="AC16GM_04830"
     CDS             complement(<1..1790)
                     /locus_tag="AC16GM_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HIR2923038.1"
                     /translation="MKGLKPEDINIILATDCGSTTTKAILIEKRGSEYRLIVRGEAPT
                     TVEAPFEDVTMGVLNAVAEVEELSGRKLIDEKGQIISPAKGNVGTDIYVSTSSAGGGL
                     QMMVAGVVRSMTAESAERAALGAGAIVMNVIASNDKRLPHQQIERIRRLRPDMILLSG
                     GIDGGTTTHVVEIAELVSAADPRPRLGHGYKLPVIFAGNKEAREPVKQTLAGKTDLTV
                     VDNLRPVLEWENLLPAREKIHDLFMEHVMAQAPGYRKLMSWTDAPIMPTPGAVGLIMK
                     RIADEEGIEVVGVDIGGATTDIFSVFQGVFNRTVSANLGMSYSISNVFAEATLPNVMR
                     WVPFVMDERDLRNRVKNKMIRPTTIPQMLEELIFEQAIAKEALRLAFIQHKSFATTLK
                     GIQQQRTIAEAFEQGASGLTLVNMMTLNMLIGSGGVLSHAPRRQQAALMLIDAFLPEG
                     VTRLSVDSIFMMPQLGVLTEVHSRAALEVFRKDCLIHLGTCVAPVGQDKEGREILDFR
                     FEMPDGKATEGKLKFGEMVRIPLGFDDKEVTLKAKAVLNPTRNFDVGAGRGAKLETTV
                     SGGVTGIILDGRGRPFNLPTDPQTRVRKLT"
     gene            complement(1944..2351)
                     /locus_tag="AC16GM_04835"
     CDS             complement(1944..2351)
                     /locus_tag="AC16GM_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923039.1"
                     /translation="MRKALLVLAGLLLAASAAQAQQRRTISPVGTGVKGNPTLEFMIG
                     PMFGSGTLRGFDDPRTFEQDLNYTGFFLNSGLTLPIQPRVALLFKGDFNWLRSKADFP
                     VVVSPVGAVQDIFEIQRTAGVVKLGFGVRIELK"
     gene            complement(2421..2843)
                     /locus_tag="AC16GM_04840"
     CDS             complement(2421..2843)
                     /locus_tag="AC16GM_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923040.1"
                     /translation="MDFRHAFSVSGSQPKGAPLTPEQEAILDKIARKVVHWKMTVPAI
                     MFLETSKPLSFLGSQTMVFFEPVIQSIFSFKDYDTVRKLLEERENVERLLLKIEQFDA
                     EALQAEKTAKDQKPSAQVTFRKRVARLFGRNRPPTPLR"
     gene            complement(2851..3306)
                     /locus_tag="AC16GM_04845"
     CDS             complement(2851..3306)
                     /locus_tag="AC16GM_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923041.1"
                     /translation="MSEVALAWTTFPFTRNWKVSGAVILFLLVVFFFVYQWIHSILFL
                     TICMVLVIGSLAGFFFPTEFQLTETGLVVRSLFGTQERPRTSFRSFYPDRNGVLLSPF
                     PRPSRLENFRGIYVRFDNNREQVLAFVQQRLGCGEITAPRSDTLASHTG"
     gene            complement(3314..3535)
                     /locus_tag="AC16GM_04850"
     CDS             complement(3314..3535)
                     /locus_tag="AC16GM_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923042.1"
                     /translation="MSNDPNRKFALKLIIGGKEREVTFEELALSNNLAQEALVRILIQ
                     KKIFTPEELLKAMEEVKKERYRSAGEAGG"
     gene            complement(3646..>4081)
                     /locus_tag="AC16GM_04855"
     CDS             complement(3646..>4081)
                     /locus_tag="AC16GM_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923043.1"
                     /translation="LDAFSPYEKHRHQDLAQQRFPDLRHRKSRLPPQPRLSLESKGSK
                     RVWAVQSHYDHPGWYIVWRYLVDPTSSVSPGRRVVIWRVDVVFLDKAGWKYEGSTASE
                     AGGGRTHTFGVRNPAKILQDKSVFLHPKIVVRGGKPTPTNGE"
BASE COUNT          849 a         1224 c         1113 g          895 t
ORIGIN      
        1 tcggtcagtt tccgcacccg ggtttgcgga tcggtgggga ggttgaacgg cctgccccgg
       61 ccatcgagga taataccggt caccccgccg gagacagtcg tttctaactt cgcgcctctg
      121 cctgcgccaa catcaaaatt tcgcgttgga tttaagaccg ccttggcctt cagcgtcact
      181 tctttgtcgt caaatcccaa aggaatgcga accatctcgc cgaattttaa ttttccttcg
      241 gtggccttgc catccggcat ttcgaatcgg aaatcgagaa tctcccgccc ttctttatcc
      301 tgccccaccg gcgccacgca ggtgcccaag tgaatcaggc agtccttgcg aaagacctcc
      361 agagccgccc gggaatgcac ttcggtgaga acccccaact gcggcatcat gaaaatcgaa
      421 tcgaccgaaa ggcgcgtcac cccttcgggc aaaaaggcgt cgatgagcat caaggctgcc
      481 tgctgccgcc gcggcgcgtg ggagagcacc ccgccggagc cgatcagcat gttcaaggtc
      541 atcatgttca ccagggtcag acccgaagcc ccttgctcga acgcctccgc aatcgtcctt
      601 tgctgctgga tgcctttgag cgtcgtggcg aacgacttgt gctggatgaa ggccagccgc
      661 agcgcttctt tggcgatggc ctgttcgaaa atcaactcct ccagcatctg cgggatggtg
      721 gtgggccgga tcattttgtt tttgacccgg ttgcgcaggt cgcgctcatc catcacgaac
      781 ggcacccagc gcatcacgtt gggcaaggtc gcctcggcaa agacgttgga aatcgagtac
      841 gacattccga ggttggccga aaccgtccgg ttaaacacac cctggaaaac agagaagatg
      901 tcggtggtcg ccccgccgat atcgacgccg acgacctcga tcccctcttc gtccgcgatc
      961 cgcttcatga tcaaaccgac cgcacccggc gtcggcataa tcggtgcgtc ggtccaggac
     1021 atcagtttcc ggtaccccgg cgcctgcgcc attacgtgct ccataaagag atcgtggatt
     1081 ttctcccgcg ccgggaggag attctcccat tccagcaccg gccgcaggtt atcgaccacg
     1141 gtcaggtcgg tcttgccagc cagtgtttgc ttgaccggct cccgcgcctc cttgttcccg
     1201 gcaaaaatca ccggcagttt atacccgtgc cctaaacgcg ggcgtgggtc agccgcgctg
     1261 accaattccg cgatttcaac aacgtgcgtg gtggtccccc cgtcaatccc gccggagagg
     1321 agaatcatat ctggccgcag acggcggatg cgttcgattt gctggtgcgg caggcgcttg
     1381 tcgttggaag cgatcacgtt cattacaatc gccccggcgc ccagggccgc ccgctccgcc
     1441 gactccgcgg tcatcgagcg caccaccccg gccaccatca tctgcaagcc gccgccggcg
     1501 gaggaggtgg agacgtaaat atcggtgccg acattcccct tcgccggaga gatgatctgc
     1561 cccttttcgt cgatcagctt ccgtccggag agttcttcca cttcggccac ggcattgagg
     1621 actcccatgg ttacgtcctc gaacggcgcc tcgacggtgg taggcgcttc cccccggacg
     1681 atgagccggt actcgctccc gcgcttttcg atcaggattg ctttggtggt ggtcgaaccg
     1741 cagtcggtgg ctaaaatgat gttgatgtct tcgggcttga gacctttcat tgtttcctct
     1801 atcttgaagt agagtcctgc aaaaacaaaa tcacccttcg gcacccgccg gggagttgcg
     1861 gcagaatata caaaaaaaca ggcaacccac cacccctttt ggggatggtc ggtggcctgc
     1921 ttttgatctg acgcctgtgg cgcttacttc agctcgatcc gcacgccgaa gccgagtttg
     1981 acgacgccgg ccgtgcgctg gatctcaaat atgtcttgga cggccccgac cggactgacg
     2041 accacgggga aatcggcttt cgagcgcagc cagttgaaat cacccttaaa caggagcgcc
     2101 acccgtggct gaatgggaag tgtcagcccg ctgttgagga agaagccagt gtaattgaga
     2161 tcctgttcaa acgtgcgcgg gtcatcaaac ccgcgcagcg tcccggaacc gaacattggt
     2221 ccaatcataa actccagcgt ggggttgcct ttgacgccgg taccgaccgg gctgatcgtt
     2281 cgccgctgct gcgcctgcgc tgcgcttgcc gcgagcagaa gccctgccag gacgagcaat
     2341 gccttccgca tggtgcctcc tttgggttta aggttggttt ccgtacctac ccgtacttgt
     2401 cggcgtatct ggagcttcgc ttacctaagg ggagtgggcg gtcggttgcg cccgaacagc
     2461 cgcgcaacgc gttttcggaa cgtcacctga gccgacggct tctggtcctt tgccgttttc
     2521 tcggcctgca gcgcctccgc gtcgaactgt tcaattttca aaagcaaccg ttcgacgttt
     2581 tcccgctctt ccaagagttt gcgcaccgtg tcatagtctt tgaaggagaa gatcgattga
     2641 atgaccggct caaaaaaaac catggtctgg ctgcccagaa aggagagggg cttggaggtt
     2701 tccagaaaca tgatcgccgg gaccgtcatc ttccagtgga ccaccttacg ggcgattttg
     2761 tccaatatcg cttcctgctc cggcgtgagc ggggctcctt tgggttggga accggatacc
     2821 gaaaaagcgt ggcggaagtc cacggtgact ttacccggtg tgcgacgcga gcgtatcgga
     2881 acggggtgct gtgatctccc cgcaccccaa ccgctgttgt acgaacgcca gcacctgctc
     2941 ccgattgttg tcaaaccgca cgtaaatccc ccggaagttc tccaaacgcg acgggcgcgg
     3001 gaaaggcgaa agcagaacgc cattccgatc cgggtaaaaa ctgcggaagg acgtcctggg
     3061 ccgctcctgc gtgccaaaaa gcgaccgcac gaccagcccg gtctcggtca gctggaactc
     3121 ggtggggaag aaaaacccgg ccagagagcc gatcaccagc accatgcaga tggtgaggaa
     3181 aaggattgaa tgaatccact ggtagacgaa gaaaaaaacc accaaaagga aaaggataac
     3241 cgcccccgaa accttccagt tccgggtgaa ggggaaagtg gtccaagcca gcgcaacctc
     3301 ggacatgcag acgctatcct cccgcttctc cggcactccg gtagcgctct tttttcactt
     3361 cctccatcgc tttcaagagt tcttcagggg taaaaatctt cttttgtatc aaaatccgca
     3421 ccagcgcctc ctgggctaag ttgttggaga gggccagttc ttcaaaggtc acctcgcgct
     3481 ctttcccgcc gatgatgagt ttcagggcga acttgcggtt aggatcattc gacacgtgaa
     3541 ctccaaaagt gtgaaggttc taaggtctta ctttttcccg gtattgtttc cttcggacca
     3601 aaagtggaac attggttcaa ttccaatcta tgtcttgact tagacttatt ctccattggt
     3661 gggtgtaggt tttccgccac gtacaacgat tttcggatga aggaaaacgg atttgtcttg
     3721 aagtattttt gccgggtttc tgaccccaaa agtgtgagta cgccctcctc ccgcctcact
     3781 tgccgtactt ccttcgtact tccaaccggc cttgtccaga aaaactacat ccactcgcca
     3841 gatgacgaca cgtctacccg gcgagacgct acttgttgga tcaacgagat aacgccagac
     3901 gatataccac cccgggtggt cgtagtggga ttggacggcc catacgcgct tgctcccttt
     3961 agattctaag gacaatctag gctgcggcgg caaccgactc ttacggtgcc tcaaatccgg
     4021 aaaacgctgt tgagcaagat cttgatgtct gtgtttttcg taaggactaa aggcatctag
     4081 c
//
LOCUS       DBNGZD010000180         4542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_29342, whole genome shotgun sequence.
ACCESSION   DBNGZD010000180 DBNGZD010000000
VERSION     DBNGZD010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_29342"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="AC16GM_04860"
     CDS             complement(<1..1608)
                     /locus_tag="AC16GM_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR2923044.1"
                     /translation="MRQRTVFLTVIGFLLLPTGVFAFQPLFDTWLAYDVGSIQKGIFA
                     ADVDGSGGLDLLVADYGTNTVSVLLNNGDKTFAPKVNYATGTRPDAVFAADLDGDGDQ
                     DLAVVNYGSRTVSILKNNGNGTFAVKVDYPTGNGPKSIYIADLDGDGDRDLAVANDSS
                     NTVSILKNNGNATFAAKVDYAAGTNPASVFGANLDGDGDIDLAVANAGSDNVSILLNN
                     GDGTFAAKVDYSSPGNPFSIYAADLDGDGDRDLAVASGFVSGSGFRIFKNNGNGTFAA
                     GVGYSVPTGGGGGLSSVVLTDVDGDGDQDLVVANPATSMVHVGKNDGTGTFFSGPTIW
                     ADYPSGPGPLALFAADLDGDTYPDLAAANGGTNSISVHRNDGNGAFDRRQDINVGSGG
                     HSVYAADLDGDLDRDLAVESGYGFAIYKNGGDGTFGSGAFYGAGGAFTSDLFAADFDG
                     DGDQDVAVTGSSINFPYTYFVSIWKNNGDGTFAGRVDYTTGSGPQTVFAADLDGDGDR
                     DLVTANQTVDKVSILKNNGDGTFALHVD"
     gene            complement(1913..3373)
                     /locus_tag="AC16GM_04865"
     CDS             complement(1913..3373)
                     /locus_tag="AC16GM_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2923045.1"
                     /translation="MTSYFRFSLQRSLGCGILLFWPFFHSSAFGQVTPLYQKDGAAAG
                     DNLGYSVAGAGDVNGDGKADFIIGASRANPGGRVVAGSAYVYSGADGSLLYQKNGAAA
                     GDRLGNSVAGAGDVNGDGKADFIIGAYNADPGGRSNAGSAFVYSGADGSLLYQKDGAA
                     AGDFLGFSVAGGGDVNGDFIIGAYTADPGGRVDAGSAFVYSGATGALIYQKDGAAAND
                     NLGYSVAGAGDVNGDAKADFIIGATNADPGGRVDAGSAYLYSGADGSLLYQKDGAASG
                     DRLGNSVAGAGDVNGDAKADFIIGAHNADPGGRVNAGSAYVYSGADGTLLYQQDGAGI
                     DDLGNSVAGAGDVNGDTKADIIIGAFLASPAGRSNAGSVYVYSFSPTGVTREEIAGIP
                     ATFTLEQNFPNPFNASTQIVFDLPGAGGRAVKLEVIDILGQRVATLIDQTLAGGRYHV
                     TWDGKDGQGKTVASGVYFYRLQAGEYQETKRMMLLK"
     gene            complement(3565..>4542)
                     /locus_tag="AC16GM_04870"
     CDS             complement(3565..>4542)
                     /locus_tag="AC16GM_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2923046.1"
                     /translation="IFGFSVSGAGDLDGDGRGDFIVGACSADPGGRPGAGSAYIYSGA
                     TGALLYQKNGASNSVELGWSVSGAGDVDGDGRDDFIIGAPSVDFGGIGSVFVYSGTTG
                     ALLAQKVAPVAGSDFGITVAGTGDVDGDGKGDYAIGTSRANVSGIGNAGSVLVYSGGS
                     GALLHQINGSAAGDSLGFWVADAGDVNGDGKTDIIIGAPRTDPGGITDAGSVYVYSFS
                     PTGVTRVEFAGLPSSFTLSQNFPNPFNANTQIVFDIPGVESKSVRLEVFDILGQRIAT
                     LIDEPLSGGRYHITWDGRDGEGKSVASGVYFYHLTAVEFQEAKRMTLLK"
BASE COUNT         1020 a         1390 c         1231 g          901 t
ORIGIN      
        1 atctacgtgg agagcaaaag tcccatcccc gttgttcttc aagatggaaa ccttgtcaac
       61 cgtttggttt gccgtaacca ggtcccggtc gccgtcccca tccaagtcgg ccgcgaaaac
      121 cgtctgcggg ccactccccg tggtatagtc aaccctgccg gcaaacgtcc cgtccccgtt
      181 gttcttccaa attgaaacga agtaggtata ggggaaatta atagagctac cagttaccgc
      241 cacatcctgg tcgccgtccc cgtcaaaatc tgcggcaaaa aggtctgagg tgaacgctcc
      301 cccggcaccg tagaaagccc cggagccgaa ggttccatcc cctccattct tgtaaatggc
      361 aaagccgtag ccgctttcca cggccaaatc ccggtccaaa tccccgtcca agtccgcggc
      421 gtaaacggaa tgaccaccgc tgcccacatt gatatcctgc cggcggtcga acgctccgtt
      481 cccgtcgtta cggtggacgg aaatgctgtt ggtccccccg ttggccgcgg ccaaatccgg
      541 atacgtatcc ccgtccaaat ccgccgcgaa gagggcgagc gggcctggtc ccgaaggata
      601 gtcggcccag atcgtcgggc cggaaaaaaa agtacccgtc ccgtcgtttt tgccgacatg
      661 gaccatggag gtcgccggat tggcaacgac caaatcctgg tcaccgtccc cgtccacatc
      721 ggtcagaacc acggaggaca aaccgccgcc accgccggtc ggtactgaat atccgacgcc
      781 ggcggcaaaa gtgccgttcc cgttgttttt gaaaatgcga aaaccggagc cgctgacaaa
      841 cccacttgcc acggccaaat cccggtcgcc atccccgtcc aaatccgccg cataaataga
      901 gaatggattg ccggggctgg agtaatcaac cttggcggca aaagttccgt ccccgttgtt
      961 taagagaatg gaaacgttgt cgctgccggc attggcgaca gccaagtcga tgtcgccgtc
     1021 cccatccaga ttggcaccaa agaccgaggc cggattggtg ccggccgcat agtcaacttt
     1081 ggccgcaaag gtggcgtttc cgttgttttt cagaatggat accgtgttgc tgctgtcgtt
     1141 ggccaccgcc aaatcccggt ccccgtcccc gtccaaatcg gcaatgtaaa tggatttggg
     1201 gccgttgccg gtagggtagt ccaccttaac cgcgaaggtg ccgttgccgt tgtttttcag
     1261 aatggaaacc gtgcggcttc cgtagttcac caccgccaag tcctgatcgc catccccatc
     1321 caaatcggcg gcaaagaccg catccggacg ggtgccggtg gcgtagttga ccttgggggc
     1381 gaaggttttg tcgccgttgt tcagaagaac cgaaacggtg ttggtaccgt aatcggccac
     1441 cagcaaatcc aaaccgccgc tcccatccac gtcggcggcg aaaatccctt tctgaatact
     1501 gcccacgtca taggctaacc aagtgtcaaa aagcggctga aaggcaaaca cgccggttgg
     1561 caataacaag aacccaatca ctgtcaagaa gaccgttctt tgccgcatca caccctcctc
     1621 aagcgaatca cacggttgaa tgagtagaaa aaactacatg acgtcgtaat aaacggcgct
     1681 ttccctcctt gtcaatcgaa attccaccct tttggggagc ttttccctcc cctctgaggg
     1741 gagggtcagg gaggggtgaa acaaacatag gtagaccggt cgaagcgacc cgtatgggct
     1801 cgttctcgta cgagtcttac cgactggagg cgatcgagag aaagaaaatt ttccctcccc
     1861 ttgaggggag ggtgaggtcg aagatccgcc tcaggcgggg aggggtcata ccctacttca
     1921 acagcatcat cctctttgtc tcttggtact cccccgcctg tagccggtaa aaatacaccc
     1981 cgcttgccac cgttttcccc tgcccatctt tcccatccca ggtgacgtgg tacctcccgc
     2041 ccgccagcgt ttggtcaatg agcgtcgcaa ctctctgccc aagaatgtca ataacctcta
     2101 acttcaccgc tctgcctccc gctccgggca aatcaaagac aatctgcgtg ctcgcattga
     2161 acggattggg gaaattttgt tcgagggtga aagttgcggg aattcccgca atctcctctc
     2221 tggtcacacc ggtgggggag aaggagtaca catagaccga gccggcattg ctacgtccgg
     2281 ccgggctggc caagaaagcg ccgatgatga tatcggcttt tgtatccccg ttgacatccc
     2341 cggccccggc gacggagttg cccagatcgt caatgccggc gccgtcttgt tgatagagga
     2401 gggtgccgtc ggcgccggaa taaacgtaag ccgagccggc attgacacgc ccgcccgggt
     2461 cggcattgtg ggcgccgatg atgaaatcgg cctttgcatc gccgttgacg tccccggccc
     2521 cggcaacgga gttgcccaga cggtcaccgg aggcggcgcc gtctttttga tagaggaggg
     2581 agccgtcggc gccagaatag agataagccg agccggcatc gacacgtcca cccgggtcgg
     2641 cattggtggc accgatgatg aaatcggcct ttgcatcgcc gttgacatcc ccggccccgg
     2701 cgacggagta gcccagatta tcattggcgg cggcgccgtc tttttgatag atgagggcac
     2761 cggtggcgcc ggaatagacg aaagccgaac cggcatcgac acgtccgccc gggtcggcag
     2821 tgtaggcgcc gatgatgaaa tcgccgttga cgtccccgcc cccggcgacg gagaagccca
     2881 gaaagtcacc ggcggcggcg ccgtcttttt gatagaggag ggagccgtcg gcgccggaat
     2941 agacgaaagc cgagccggca ttgctacgtc cgcccgggtc ggcattgtag gcaccgatga
     3001 tgaagtcggc cttgccatcg ccgttgacgt ccccggcccc ggcgacggag ttgcccagac
     3061 ggtcaccggc ggcggcgccg tttttttggt agagaaggga gccgtcggct ccggaataga
     3121 cataagccga gccggcaacg acacgtccgc ccgggtttgc cctcgaggcg ccgatgatga
     3181 aatcggcctt gccatcgccg ttgacgtccc cggccccggc gacggagtag cccagattgt
     3241 caccggcggc ggcgccgtct ttctggtaca gcggggtaac ctggccaaaa gcagacgaat
     3301 gaaaaaaagg ccaaaagagg aggattccac atcccaaact gcgctggagg gaaaaccgga
     3361 aataactggt catacggctt ctccttgaat agaaggtaaa gtgagtgagg cgcccgcaag
     3421 tgtttccatt tagcaaactt tcacttcgtg aagcaaatct cagcgagggt ttaatataat
     3481 aggacttatt tagttgtcaa ggaagatctg gccgttgcag gattttaccc ttcccattga
     3541 ggggagggca gggggggtca ttccctactt caacagcgtc atccgcttcg cttcttggaa
     3601 ttctactgcg gtgagatgat agaaataaac tccgctggcc accgacttcc cctccccgtc
     3661 ccgcccatcc caggtgatgt gatacctgcc cccgctaagt ggttcatcaa tcagcgtcgc
     3721 aattctttgc cccagaatgt cgaaaacttc cagccgcacg gatttacttt caactcccgg
     3781 aatatcaaaa acaatctgtg tgttcgcatt gaacggattg gggaaattct gcgagagggt
     3841 aaaactcgaa ggaagtccag caaactccac tctggttacg ccggtggggg agaaggagta
     3901 gacatagacc gagccggcat cggtgattcc gcccggatca gtgcggggag caccgatgat
     3961 aatatcggtt ttcccgtctc cattcacatc acccgcatct gcgacccaaa accccagact
     4021 gtcgccggcg gcagacccgt ttatctggtg aagaagagca ccactcccgc cggaatagac
     4081 cagaactgat cccgcgtttc caatcccgct tacgttcgca cgcgaagtgc cgattgcgta
     4141 atcgcccttt ccatctccat ccacatctcc agtgcctgca accgtaatac cgaaatcact
     4201 tcccgccaca ggggcaactt tctgagccag caaggcacct gtggtaccgg agtaaacgaa
     4261 tactgagccg atgcctccaa agtctacact gggagcacca atgatgaaat catccctgcc
     4321 atccccatcc acgtccccgg ctccactcac cgaccagccc agttcaaccg agtttgaagc
     4381 gccatttttt tggtaaagca gtgcacccgt tgcacctgag tagatgtacg ccgagccggc
     4441 tcccggacga ccacctgggt cggcactgca cgccccaacg ataaaatcgc ctctcccatc
     4501 accgtcgaga tctccggcac cgctcacgga aaacccaaaa at
//
LOCUS       DBNGZD010000181        10310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1418820, whole genome shotgun sequence.
ACCESSION   DBNGZD010000181 DBNGZD010000000
VERSION     DBNGZD010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1418820"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..613)
                     /locus_tag="AC16GM_04875"
     CDS             complement(125..613)
                     /locus_tag="AC16GM_04875"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2923047.1"
                     /translation="MTEKEMFLQTWEREFPTTLKVLKAFPPNKGDFKPHERSLSAKQL
                     GWIFVQAQVGIDQVLKGTFTLPPQFPPVPETWEEVLMTYEKSHNGLVSQLRKLSDADL
                     NKPMQFLLGPGKMGEIPKMQFLWFVLSDNIHHRGQLSVYVRMAGGKVPSIYGPSADEP
                     WM"
     gene            complement(819..1274)
                     /locus_tag="AC16GM_04880"
     CDS             complement(819..1274)
                     /locus_tag="AC16GM_04880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR2923048.1"
                     /translation="MTRSKIKKGSPKISTAERARQVLKVVKQVPVLHYLGIKPVRLGR
                     GTSTFAVRFTQRLAQVQGILHGGILATLADTAATFASHMLLVSPQETITIELKINYLR
                     PVGSGRAIARARVLHCGQRTTVTVVDVKNQKGQLCATVLVTNLIIVRRI"
     gene            complement(1271..2140)
                     /locus_tag="AC16GM_04885"
     CDS             complement(1271..2140)
                     /locus_tag="AC16GM_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR2923049.1"
                     /translation="MHRPAVIFALTGNLLISCGTFLVAKYALSEIAPLHLAFLRFLLA
                     SGFLYAIHRFVIKGQKLAKGDRWKILFLGFIAIPINQGFFLWGLSHSTPTHSALFYST
                     TPAFVLLFALFLAQEKVTLRKTLGIGLAVVGVIIVLLEHGLVFDRRYLFGDFLLVIAT
                     MAWALYTVLGKSYLQRYGAISLTTFAMLSGTLIFSPLGLYGISSFDYHTVTVKGWLAL
                     FYLSFVTSVVAYSLWYYALARIEATKVAVVNNFQPVITALFSAWLFGEQFSTHFILGG
                     AVVLAGVFVVELA"
     gene            complement(2144..3322)
                     /locus_tag="AC16GM_04890"
     CDS             complement(2144..3322)
                     /locus_tag="AC16GM_04890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR2923050.1"
                     /translation="MTEKIAQVQSYLKAQDLSGWLLYDFRRMNPIAVEFLGITGIQTR
                     RWFYFIPAQGTPTAIIHKIEAHNFDSIPGNKVQFAGWKELETALQTVLKGAKIVAMEY
                     SPRNAIPYISRVDAGTIELVRSLGVEVVSSADLVQYFQARLTDEQYQTHLAAAKALME
                     IKDSTFALAAEKVKTGQPFSEYDLQQYMWQLYQRYGLVADAPPIVAVNANAGNPHYEP
                     TLEKHAPIQKGDLLLIDLWAKQKAPGAIYGDITWVAYLGDRVPEKFAKIFAIVRDARD
                     RAIQFVQEQVKKGVPLYGWQVDDVSRGHIASQGYGDYFTHRTGHSIGTEDHGNGVNID
                     NFETRDQRQIIPGVCFSIEPGIYLPEFGLRSEIDMFVSAGEGRVTTLPLQTEILPLLK
                     "
     gene            complement(3469..4407)
                     /gene="rfbD"
                     /locus_tag="AC16GM_04895"
     CDS             complement(3469..4407)
                     /gene="rfbD"
                     /locus_tag="AC16GM_04895"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR2923051.1"
                     /translation="MKTVLVTGAGGLLGQKLVGVLAPEWHVVAASHRPFEWQEQNVTP
                     EHLDIAIAAESQRIFEQHSPAVVINAAAYTDVDGAEDHREEAYRANVTGPENLAWLCR
                     QMNIPLVHISTDYLFDGENGPYAEEANPNPLGYYAKTKLWGEEAIIKSGCRYLIVRSN
                     VLYGTAPGVKRNFVLWVIDELQNRRTIRVVSDQYNNPTLADNLAEAIREAVEKGITGL
                     LNIAGSTYLSRHEFALAIAQRFGFDKKLVVPMLTADFKQKAPRPLRGGLRIEKAQKLL
                     ATRLLSVPEQLEILQGQLSATRTSPAPPEELRQVKK"
     gene            complement(4404..5438)
                     /gene="rfbB"
                     /locus_tag="AC16GM_04900"
     CDS             complement(4404..5438)
                     /gene="rfbB"
                     /locus_tag="AC16GM_04900"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR2923052.1"
                     /translation="MAGQKQKILVTGGCGFIGSNFVRHLLTTRDDLSLINLDKLTYAG
                     NPENLADLQEHPNYRFIHGDICDGKQVEAVLSEGIDAVVNFAAESHVDRSLYDPAVFI
                     RTNVGGTQVLLEQAVKHKVRRFVQISTDEVYGDLEPQALATESFPLRPSNPYAASKAG
                     ADLLAFSFHRTFGLDVVITRCGNNYGPYQFPEKLIPLFITNALEDKPLPLYGDGLNVR
                     DWIYVTDHCRGIELVMERGRSGKVYNISARTQKNNRDITSAILKALDKPENLIQPVKD
                     RPGHDRRYANDPGKIERELKFKPRFPFEKALAATIQWYRDNRTWWERVKSGEYQKFYE
                     KHYSASGRLK"
     gene            complement(5456..5914)
                     /locus_tag="AC16GM_04905"
     CDS             complement(5456..5914)
                     /locus_tag="AC16GM_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HIR2923053.1"
                     /translation="MIHGVKTKQLKVIPDERGRLMEVLRSDDKDFFKKFGQVYVTTAY
                     PGVVKGWHYHKHQIDTFTVIKGMMKFVLYDSRENSPTKGEVNEFFVGEHNPLLVQIPS
                     GVYHGFKCISDTEAIAINIPTEVYHYKDPDEYRLPAHTDQIPYKWAREDK"
     gene            complement(5937..6461)
                     /locus_tag="AC16GM_04910"
     CDS             complement(5937..6461)
                     /locus_tag="AC16GM_04910"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2923054.1"
                     /translation="MSFAVEQSVVIKRSLIPIWHFWIDLPQWPRWHTGLLDAYWLTSE
                     LWWKKGSQFVLVWQGKFPLNRYKVRCTVTSVEPGKSITWRAKGIGLKAMRGYRFSEVS
                     GGTQVTAYGKFNGPAAVLLRPLARAHIKMRLTQALAGLAKMVEQEVLVGKPKVERVEV
                     PPEWLVTGKKYLPF"
     gene            complement(6458..7213)
                     /locus_tag="AC16GM_04915"
     CDS             complement(6458..7213)
                     /locus_tag="AC16GM_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2923055.1"
                     /translation="MKGVILAGGLGSRLFPLTKITNKHLLPVYDQPMIYYPIQTLVEA
                     GIKDILIVTGGNSAGDFLRLLGNGKEFGLKHIDYTYQEKEGGIAEAIGLAEHFAEGEK
                     ILVMLGDNIIFESIKEAVEAFKFQKRGAKIFLKPVKDPEAYGVAELKTGKVTRIVEKP
                     RRPKSNYAVVGIYMYDNQVFEIVKTLQPSGRGELEVTDVNNAYLAQGELTHAILRGWW
                     GDAGESIEALLEANRVVAEYRQNQAKVKRARKK"
     gene            complement(7321..8088)
                     /locus_tag="AC16GM_04920"
     CDS             complement(7321..8088)
                     /locus_tag="AC16GM_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR2923056.1"
                     /translation="MQAVTLAVKARSEKGKEIVKKMRRQGVIPGVVYGPEGEPTPVAV
                     EAKVFDNLLRSGLSENAIIDLSITNGGQSTKKVLIREVQLHPVSGQILHIDFQHITLT
                     RKITVNIPVHVVGLAVGVKDTGGILQHTLRELAVSCLPTEIPEKVDVDVTALKIGDSI
                     HVREIKIEKAEILNDPNSSVVSVVPPTIIKEEELAAAAPAAEVTEPEVITEKEAQERA
                     AAKAEAEKVEGKAEKKAPPTAGEAKAEKKEEKKSEKK"
     gene            complement(8173..9177)
                     /locus_tag="AC16GM_04925"
     CDS             complement(8173..9177)
                     /locus_tag="AC16GM_04925"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR2923057.1"
                     /translation="MTPVLTERRPRTGEKPEVARTVSETPRIKILTGNSNRKLAERIA
                     AYTKIPLSECEVSRFSDGEIFVQINENIRGADVFIVQSTNPPAENLMELLLMIEAARR
                     ASAKRITAVIPYFGYARADKKDQPRVAISARLVADLITVAGANRVITMDLHASQIQGV
                     FNIPSDHLYASPVFNRYFMNLGLTNLVIVSPDVGGIRITRAFAKKFEAPLAIVDKRRP
                     APNHAEVLNIIGEVEGKTVIIRDDLVDTAGTLTQAAQALADHGAKEIYACCTHPVLSG
                     NSLELIAKSKIKKMIVADTIDTAQKKLPRQIEILSMADLFGESILRIAREESVSVLFD
                     "
     gene            complement(9347..10291)
                     /locus_tag="AC16GM_04930"
     CDS             complement(9347..10291)
                     /locus_tag="AC16GM_04930"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HIR2923058.1"
                     /translation="MLLLVLVAGVFGLVLSQENKEKMTNNKLTPEEERVIVHKGTERP
                     FTGKYYDFDEKGTYTCKRCGALLYRSDDKFDSHCGWPSFDDAIPGAVKQVPDADGMRT
                     EILCAKCGAHLGHVFTGEKFTAKDTRYCVNSISMNFIPAGAGSSKPEAKTQTQTQKAY
                     FAGGCFWGTEYYLKQAKGVVDVKVGYMGGHTKNPTYQQVCSGTTGHIETNEVTFDPAK
                     TSYEEVAKLFFEIHDPTQVNRQGPDIGEQYRSVVFYADDGQKQTAEKLIGILKKKGFK
                     VATELRPASAFWVAEDYHQDYYTKKGGEPYCHTRVKRF"
BASE COUNT         2095 a         2939 c         2886 g         2390 t
ORIGIN      
        1 tattggccgg gtaggccttt aacaccttga gcgtggtttt gaactcccgc tcccaggtct
       61 tcaggaacat atccttttca ttcatagcca gttcctctcg agtgatacgg ttgaacgcca
      121 ataactacat ccagggctcg tccgccgagg gcccgtagat tgagggcact ttgccgcccg
      181 ccatccggac gtaaacggag agctgtcccc ggtgatggat gttatcggac aagacaaacc
      241 acagaaactg catcttgggg atttcgccca tcttcccggg cccgagcaaa aactgcatcg
      301 gcttgtttag gtcggcatct gagagtttcc gcaactggct caccaggccg ttgtgcgatt
      361 tttcataggt catcaggact tcctcccatg tttccggcac gggcggaaac tggggcggaa
      421 gcgtgaaggt gccctttaag acttggtcga tccccacctg tgcctgaacg aaaatccacc
      481 ccagttgctt ggcggatagg gagcgttcgt gcggcttgaa atcccctttg ttgggcggaa
      541 atgccttcag cactttcagc gtggtgggaa actcgcgctc ccaggtttgg aggaacatct
      601 ctttttcggt catactctgg ctcctttccg cgttgagagg ttattgcggt ttcgagaata
      661 cttaaccagg tagttaagta taaaggcgga tactctactt gtcaagtcct aatttcaaaa
      721 atctttctat ttttaagaac ggctgccggg tagtggtaac cgcttgttgg acaaaaggat
      781 agctccccat tcggggaaac ggtcggtgaa atccgttttc agattcttcg aacgatgatc
      841 agattcgtca ccaaaacggt agcgcagagt tgtccctttt gattttttac atcaaccacg
      901 gtcaccgtcg tgcgctggcc acagtggagc acccgcgcac gggcaatcgc gcgaccggaa
      961 ccgacgggac gcaggtagtt gatcttcagt tcaatggtga tggtttcttg tggagagacc
     1021 aaaagcatgt gggaggcaaa agtcgccgcc gtatcggcga gggtagcgag aattcccccg
     1081 tgcaaaatgc cctggacttg agccaggcgc tgcgtgaacc gcaccgcaaa ggtcgaagtg
     1141 ccgcgtccta atcggaccgg tttgattccc aaatagtgga gcaccggcac ttgcttgacg
     1201 accttgagca cctggcgcgc ccgctcggca gtcgaaatct tcgggctgcc ttttttgatt
     1261 ttggatcgcg tcacgccaac tccaccacaa agacacccgc cagcaccacc gctcctccca
     1321 gaataaaatg ggtggagaac tgctcgccaa agagccaggc ggaaaataaa gcggtaatca
     1381 ccggttggaa gttgttcacc accgccacct tggtggcctc aatccgcgcc aaggcgtagt
     1441 accagaggga ataggccacg accgatgtta caaaggaaag gtagaaaagc gcaagccagc
     1501 ctttgaccgt caccgtgtga tagtcgaaac tggaaattcc gtaaagtccc aggggcgaaa
     1561 agatgagcgt gccggagagc atggcgaacg tagtcaatga aatggcaccg tagcgctgga
     1621 ggtatgactt ccccaacacc gtgtagagcg cccaggccat ggtggctatc acgagaagaa
     1681 aatccccaaa aaggtagcgg cggtcaaaga ccaacccgtg ttccaggaga acgatgatga
     1741 cgccgaccac ggccagcccg atgcccaaag ttttgcggag ggtgaccttc tcctgggcca
     1801 agaagagcgc gaacagaagc acgaaggccg gcgtggtgct gtagaagagc gccgagtgag
     1861 tcggcgtgga gtgggaaagt ccccagagga aaaatccctg attgatcgga atggcaatga
     1921 accccaaaaa gagaattttc cagcggtcac ctttggctaa cttctgaccc ttaatgacaa
     1981 aacggtgaat ggcgtagagg aacccggaag ccagaaggaa gcgcaaaaag gccaggtgca
     2041 gcggcgcaat ttccgacagc gcgtacttgg ccacgagaaa ggtgccgcag gagatgagga
     2101 gatttccggt gagggcaaag atgacggcgg ggcggtgcac cggctatttc aggaggggaa
     2161 gaatttcggt ttgaagcggc aatgtggtga cccggccctc gcctgcactc acgaacatgt
     2221 caatctccga gcggaggccg aactccggca ggtatatgcc cggctcgatg gaaaagcaga
     2281 ccccgggaat aatctgcctt tgatcgcggg tctcgaaatt atcgatgttg accccgttgc
     2341 cgtgatcttc ggtgccgatg gagtggcctg tgcggtgcgt gaaataatcg ccgtaaccct
     2401 gggacgctat gtggccgcgg gagacgtcat ccacctgcca gccgtagagc gggacgcctt
     2461 ttttgacctg ctcttgaacg aactggatcg cccggtcgcg ggcgtcacgg acgatagcaa
     2521 atattttggc gaatttctct ggcacccggt cacccagata cgcgacccag gtgatgtcgc
     2581 cgtagatggc tcccggcgcc ttctgtttgg cccagaggtc gatcaagagc aagtcccctt
     2641 tttgaatcgg cgcgtgcttc tccagcgtcg gctcgtagtg gggattaccc gcattggcgt
     2701 tcaccgccac aatcggcggc gcatccgcta ccaatccgta acgctggtac agttgccaca
     2761 tatactgctg gagatcgtac tcgctgaacg gctggccagt cttgactttc tccgccgcca
     2821 gtgcaaaagt cgaatctttg atttccatta aagccttcgc cgcagccaaa tgcgtctggt
     2881 actgctcgtc agtcagccgg gcctggaaat actgcaccag atcggcggaa gaaaccacct
     2941 ccacccccag actgcggacg agttcaatcg tccccgcatc cacccgggaa atgtagggga
     3001 tggcattgcg cggggaatat tccatagcga ctatctttgc acccttgagg acagtttgca
     3061 aggcggtctc cagttctttc cagccggcga actgaacctt gttgcccggg atgctgtcga
     3121 agttgtgcgc ttctattttg tggatgatgg ccgtgggcgt cccctgagcc ggaatgaagt
     3181 agaaccagcg ccgggtctgg atgccggtaa tgcccaaaaa ttccaccgcg atcggattca
     3241 ttctccggaa atcgtacaaa agccagccgg aaagatcttg ggctttcaag taactctgca
     3301 cctgggcaat tttctccgtc accgttttcc ccttctcttc ggcttgcata gaaacagtca
     3361 ggcaaaatag taccacgggt cctgcgagcc aaaatttgcg catgctttga acctcccgcc
     3421 ttcaacttgc gtcaatctta cgggaaagca acttcccttt tccggtcgtc atttttttac
     3481 ctgccgcagc tcttcgggtg gggctgggct tgtcctggta gcggagagtt gtccctggag
     3541 tatttccaac tgctccggca ccgagagcaa ccgggtcgcc aaaagtttct gcgccttctc
     3601 gatgcgcaac ccgccccgca gtggccgggg tgctttctgc ttgaagtcag ctgtcagcat
     3661 cggaacaacg agttttttat cgaaaccaaa acgttgagcg attgccagag caaattcgtg
     3721 tcgggaaagg taggtgctac cagcgatgtt cagaagaccg gtgatccctt tctccacggc
     3781 ttcacggatg gcctctgcca gattatccgc cagagtcgga ttattatact ggtcgctcac
     3841 gacgcgaatc gtcctgcggt tctgtaactc gtcgataacc cagaggacga aattgcgttt
     3901 gactcccggc gcggtgccgt acagcacgtt gctgcggacg ataaggtacc tgcagccgct
     3961 tttgatgatt gcttcctccc cccagagttt cgtcttggcg taatacccaa gaggattggg
     4021 attcgcttcc tccgcgtacg ggccgttttc accgtcgaaa aggtaatcgg tggaaatgtg
     4081 cacgagggga atgttcatct ggcggcacag ccaagccaga ttctccggcc ccgtgacgtt
     4141 ggcacggtag gcttcttccc ggtgatcttc tgcaccatcg acgtcggtgt aggcggcggc
     4201 attgatgacg accgcgggtg aatgctgctc aaagatgcgc tggctctctg cggcaatagc
     4261 aatatccaga tgctcagggg tgacattctg ttcttgccac tcgaacggtc ggtgtgaagc
     4321 cgccactacg tgccactccg gagccagaac gcccaccagt ttttgaccca ggaggccgcc
     4381 tgcgcccgtg actagaacgg ttttcacttc agtcggcctg aggcggaata atgcttttcg
     4441 taaaacttct ggtactcgcc cgatttcacc cgctcccacc aggtgcggtt atcgcggtac
     4501 cactggatcg tcgcggccag ggccttctcg aacggaaacc ggggtttgaa ctttaattcg
     4561 cgctcgattt ttcccgggtc gttggcgtag cgccggtcgt gtcccggccg gtctttcacc
     4621 ggctggatca aattctccgg cttgtccaac gccttaagaa tcgcgctggt gatgtcacgg
     4681 ttattttttt gcgtccgggc gctgatgttg tagactttcc ccgaccggcc ccgctccatc
     4741 actaattcga tgccccggca gtggtcggta acgtaaatcc agtcgcggac gttcaacccg
     4801 tcgccgtaga ggggcagcgg tttgtcttcc agcgcgttgg tgatgaagag ggggatcagt
     4861 ttttccggaa actggtatgg cccgtagttg ttcccgcaac gggtgataac gacgtccaat
     4921 ccgaacgtgc ggtgaaagga gaacgccagc agatcggccc cggccttgga ggccgcgtac
     4981 gggttcgagg gacgcagggg aaaagattcg gtggccagcg cctgcggctc taaatcgccg
     5041 tacacctcgt cggtcgaaat ctgcacgaag cgcctgactt tatgtttgac cgcctgctct
     5101 aagagcacct gggtgccgcc gacgttcgtc cggatgaaga ccgccggatc gtagagactg
     5161 cgatcgacgt gcgactcggc ggcaaaattg acgacggcgt cgatgccctc ggagagaacc
     5221 gcctccacct gcttcccgtc gcagatgtcg ccgtggatga agcggtaatt ggggtgctct
     5281 tgcaggtcgg ctaagttttc gggattgccg gcgtaggtca gtttgtcgag gttaataagt
     5341 gagagatcgt cgcgggtggt caaaagatgc cggacgaagt tcgacccgat gaacccgcaa
     5401 ccgccagtga cgagaatttt ctgcttttga cccgccacac ctcacccatc gcccgtcact
     5461 tgtcttcccg cgcccacttg tagggaatct gatcggtgtg cgcagggagg cgatactcat
     5521 ccgggtcttt gtaatggtac acttccgtgg ggatgttgat ggcaatcgcc tcggtgtccg
     5581 agatgcactt aaagccgtgg tagacgccgc tgggaatctg gactaatagc ggattgtgct
     5641 cgcccacgaa aaactcgttc acctcacctt tggtggggga attctcccgc gaatcgtaca
     5701 ggacgaactt catcatcccc ttgatcaccg taaaggtgtc gatctgatgc ttgtggtagt
     5761 gccagccctt caccaccccc gggtaggccg tggtgacgta gacctggccg aattttttga
     5821 aaaagtcctt atcatccgag cgcaaaactt ccatcaagcg cccgcgctcg tcggggatga
     5881 ctttcagttg tttagttttg acgccgtgaa tcacttttga cctcgtttct ctaccattag
     5941 aaaggcaggt acttcttgcc ggtgaccagc cactccggtg gtacttcgac ccgctccact
     6001 ttgggttttc ccaccagcac ctcttgctcg accatcttcg ccaatccggc cagcgcctgc
     6061 gtcagccgca ttttgatgtg ggcacgagcc aggggtcgca acagcaccgc cgctgggccg
     6121 ttaaactttc catacgctgt cacctgcgtc ccgcctgaaa cttcgctgaa ccggtagccg
     6181 cgcatggcct ttagcccaat cccttttgcg cgccaggtga tacttttccc cggttccaca
     6241 ctggtgaccg tgcaacgaac cttgtacctg ttgagcggaa atttgccctg ccagaccaag
     6301 acgaactgcg agcctttttt ccaccagagt tcggatgtca gccagtaggc gtccagaagt
     6361 ccggtgtgcc accggggcca ctgtggcaga tcgatccaga agtgccagat gggaatcaag
     6421 gagcgtttga tgacgacgga ctgctcgacg gcgaaactca ttttttcctg gcacgtttaa
     6481 ccttcgcttg gttctggcgg tattctgcca ccacccggtt ggcttccaat agcgcctcaa
     6541 tcgactcacc ggcatccccc caccagccgc gcaggatggc atgggtcagt tcgccctggg
     6601 ccaggtaggc gttgttcacg tcggttactt ccaactcccc ccggcccgag ggttggagcg
     6661 tcttgacgat ctcaaagacc tggttgtcgt acatgtagat gccgaccacg gcgtagttgc
     6721 tcttgggccg tcggggtttt tcgacgatgc gcgtcacctt gccagtcttc aactccgcca
     6781 cgccgtaggc ctccgggtct ttcaccggct tcagaaatat tttggcgccg cgcttctgga
     6841 acttgaacgc ctccaccgcc tctttgatcg attcgaagat gatgttgtcc cccagcatca
     6901 ccaaaatttt ctccccttcg gcgaagtgct ccgccaggcc gatcgcctcg gcgatgccgc
     6961 cttctttttc ctgataggtg tagtcgatgt gcttgagccc gaattctttt ccgttgccca
     7021 agagccggag gaaatcgccc gccgaattcc cgccggtgac gatcagaatg tccttgatcc
     7081 ccgcttccac cagcgtctga atcgggtagt aaatcatcgg ctggtcgtag accggcaaaa
     7141 gatgtttgtt agtgatcttg gtgagcggga agagccggct gcccagcccg cccgcgagaa
     7201 tgacgccttt catcggttag ctttgcatct cttgaaattt ttcgcgaata tactcaaacc
     7261 cacgccgcaa aacaaaaacg ccggggcttt accccggcgt aaccgactgc ctgaccccaa
     7321 tcatttcttc tcggatttct tctcttcttt tttctccgct ttggcttccc cagccgttgg
     7381 cggggctttc ttttcggctt tgccctcgac cttctccgct tcggccttgg ccgccgcccg
     7441 ctcctgggct tctttttcgg tgatcacttc cggctcggtc acctcggctg ccggcgctgc
     7501 ggccgccagt tcctcttcct tgataatggt cggcggcacc accgagacca ccgaggaatt
     7561 cgggtcgttt aagatttccg ccttctcgat tttaatttcc cgcacgtgaa tcgagtcgcc
     7621 gattttgagc gccgtgacgt ccacgtccac tttttcggga atctcggtcg gcagacagga
     7681 aaccgcaagc tcgcgcaggg tgtgctgcaa aatgcccccg gtgtctttca ctcccacggc
     7741 taagccgacg acgtggaccg ggatgttcac ggtgatcttc cgggtgagcg taatgtgctg
     7801 gaagtcgatg tgcaggatct gcccggagac cgggtgcagc tgcacctcgc ggattaacac
     7861 ctttttggtg gattgaccgc cattggtaat cgaaaggtcg atgatggcgt tttcgctcaa
     7921 accggagcgc aaaagattat cgaagacttt ggcctcaacg gccaccggcg tcggctcacc
     7981 ctccggccca tagaccacgc cggggataac cccctgccgc ctcatttttt tgacgatttc
     8041 cttgcccttt tcgctgcggg ccttgaccgc taaggtgact gcctgcatca ttgctcctga
     8101 gaatgtgaaa tcagaacccc gcaaaccagc ggggcattgc caaatatagt ctttcccggc
     8161 ccaaatgcaa cctcaatcga aaagcaccga gaccgactct tcccgggcga tgcgcaggat
     8221 cgactcgccg aacaggtcgg ccatggagag gatttcgatc tggcgcggca gtttcttctg
     8281 ggcggtgtcg atggtgtcgg ccacgatcat ttttttgatt ttcgatttgg cgattaactc
     8341 caaagaattg cccgacagga caggatgggt acagcaggcg taaatttctt tggcgccgtg
     8401 atcggccagc gcctgcgccg cctgggtcaa ggtcccggcg gtgtcgacca ggtcgtcccg
     8461 gatgatgacg gttttcccct ccacttcgcc gatgatgttc aaaacttcgg catggttcgg
     8521 cgccgggcgg cgcttgtcca cgatggctaa aggcgcctcg aatttcttgg caaaagcccg
     8581 ggtgatgcgg attccgccca cgtcgggcga gacgattacc agattggtca ggcccaaatt
     8641 cataaagtag cggttgaaaa ccggtgacgc gtagaggtgg tcggagggga tgttaaagac
     8701 gccctgaatc tgggaggcgt ggagatccat ggtgatcacc cggttggcgc cggcgaccgt
     8761 gatgaggtcg gccacgagcc ttgcgctgat cgccacccgg ggctggtctt tcttgtcggc
     8821 ccgggcgtag ccgaagtagg ggatgaccgc cgtgatccgc ttggccgagg cccgccgcgc
     8881 cgcctcaatc atcaggagca gttccatcaa attttccgcc ggcgggttgg tggattggac
     8941 gatgaaaacg tccgcccctc ggatgttctc gttgatctgg acgaagatct cgccgtcgga
     9001 aaaacgcgag acctcgcact cggataaggg aatcttggta taggccgcaa tccgctcggc
     9061 caacttccgg ttgctgttgc cggtgagaat tttaatgcgt ggcgtttcgc tcacggtgcg
     9121 ggccacctcc ggcttctctc cagtgcgcgg tctgcgttca gtcagaactg gcgtcatggc
     9181 caccctcagt tgcgattgaa cagaaaaatg aagggcattt aagggaaaaa atggcagata
     9241 agaagtaaaa agtcaagact tgacattcga gatattttgt atgggcaaaa ataagccccg
     9301 attctcggtc ggggcttaaa gtttagaggt tgagttccag cctgatttag aatctcttga
     9361 cccgggtatg gcagtacggc tccccgccct ttttggtgta gtaatcctga tggtaatctt
     9421 ccgccaccca gaatgcggag gccgggcgca gctcggtcgc cactttgaaa cccttctttt
     9481 tcagaatgcc gattaacttt tcggcggttt gtttctgccc gtcgtccgcg tagaaaacca
     9541 ccgaccggta ctgctccccg atatccggtc cctggcggtt cacctgggtc gggtcgtgga
     9601 tttcgaaaaa gagtttagct acttcttcat acgaggtctt cgccgggtcg aaggtcacct
     9661 cgttggtttc gatatggccg gttgtgccgc tgcaaacctg ctggtaggtc ggattcttcg
     9721 tgtgtccgcc catatagccg accttcacgt caacgacccc tttggcctgc tttaagtagt
     9781 actccgttcc ccaaaaacag ccgccggcaa aataggcctt ttgcgtctgc gtctgggttt
     9841 tggcttccgg cttactcgaa cccgctccgg cggggataaa gttcatcgaa atcgaattga
     9901 cgcaatagcg ggtgtctttg gcggtaaatt tttcgccggt gaaaacgtgt cccaggtgcg
     9961 cgccgcactt ggcgcacaaa atttccgtcc gcatcccgtc ggcatcgggc acctgcttga
    10021 ccgcgccggg aatggcgtca tcgaagctgg gccagccgca gtgggagtcg aacttgtcgt
    10081 ccgagcggta gagcagggcg ccgcaccgct tgcaggtgta ggtccccttc tcgtcgaaat
    10141 cgtagtactt gccggtaaag ggccgctccg tgcccttgtg gacgatcacc cgttcttcct
    10201 cgggggtcaa cttattgttg gtcattttct ccttattctc ctgtgacaaa accaagccaa
    10261 aaactcctgc caccagcacc agtagtaaca taatcctttt caggcccccc
//
LOCUS       DBNGZD010000182         5014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_29561, whole genome shotgun sequence.
ACCESSION   DBNGZD010000182 DBNGZD010000000
VERSION     DBNGZD010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_29561"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..963
                     /locus_tag="AC16GM_04935"
     CDS             229..963
                     /locus_tag="AC16GM_04935"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2923059.1"
                     /translation="MSSKATLQYFGRDLEAMSFAENYHKWIVDEFRPYLGDSVAEIGA
                     GTGNFSKFLVAAEIKSLVAFEPSGNMYPLLKEHFKNNKKVDTVPAFLKDAVKNYQDSF
                     DSVVYVNVLEHVEDDEKELSYVFAALKEKGHLLLFVPALSWLYSKLDRNLGHYRRYHK
                     NALAKLVQNAGFSIVKVKYFDFVGILPWFIAFVLLQQSITGDSVALYDKFVVPVMRVA
                     EGVISPPIGKNLLLVGKKNGRRFGGG"
     gene            1186..3447
                     /locus_tag="AC16GM_04940"
     CDS             1186..3447
                     /locus_tag="AC16GM_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2923060.1"
                     /translation="MHVTRIALVAGLLASFCFTSAVASDGTWQRISDSPQPTVFGDGY
                     VLDLGPLQPPVPTIPGYTVTPFAGIQRPRGVSFNAAGDLFTMDRDSGTIFRITPDGAV
                     SVVADLPDILAGYVGPLFDPVSGNLFVSRFAVYTGNEVLRITPAGNVDVYATGILPPG
                     GLTSDPAGNLYVSQFEDGGQVFKITPAREVSPFASGLYYPDGLVFGPTGDLFICNTRL
                     NEIRRVPPTGGTAALFASGFNYPIAATFDNAGNLLVAEYLGGSIAKVSPTGTVSPFGS
                     GFISPAGLAFDDQGDLFIADFGANMIYKAVPGAVGQPIPALFLGRTSGLPGDTVLMPL
                     RLRPPHGLAALDATITWNPALAQCIEVHKTPATDSFLLESNLTVQGEAQVAMVSREPI
                     PRGAADELLVFSFVIEDTVSRGTTIELNIREPHLFDPNGDHIGVVLFYPSGEIFVGNP
                     PGDVDGDGRVTIADVILTLQIVVGTHTPTEIERMAADVNHDGEINSADAVIILHMVTT
                     EAATRPPVDSSAAVSFVAPQVAYNPSDQTVRLTLFGELLTDVAGGKLHVQFDPTLVAL
                     QQTAAGRDVGQTLNEFASDAANVRFAFAKSSNLTLAVGELAQVQWRALQSLTLEQLNR
                     AVRVVGTEFYGENGQSIPVAVRLSGPTGVTNQNGTVPKSYALRQNVPNPFNARTAIHF
                     SLPQPGEVALEIFNVAAQKVRTLVNAHYPAGEQVIFWDGRDDQGEELASGLYFYRLTA
                     GSFSDTRKMVLVK"
     gene            complement(3454..4737)
                     /locus_tag="AC16GM_04945"
     CDS             complement(3454..4737)
                     /locus_tag="AC16GM_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923061.1"
                     /translation="MPLSLDTIHQPLNDFFINKFKTDAGSRVFFRFDKFGSVISDDDF
                     IDPNHPESGYLPALAKEKFSDLVNQIPVDEADGLNIHFSEINQIDEFYFYHLLNPSSP
                     FFPEGIDDQSKETIMNSFNEMKADAKKLWQNIVLESSTGLILDYKPCLATPESWYDKA
                     RDELWTRHLFQASETTGAPVDNSPKFQLWKLKIDDAAIQKVLPSDESQPADPVSLYKN
                     VLSVKTIPQAMFIAHTKSNEIIQPTKPTLAAGSLSKLNINIAHELSPAVSVKSFAVHT
                     NYLQQLNMLDIRKRMLVNKYLADNAPTQPVKTSSISISFDYCIVGIDRPWILTAFIND
                     KSWFIPITAKGQLTTSGGLTAMPIGFVAIKNLNIESTNWSAEDIAISKDATDFGPFKV
                     SSEILSSKISHAGIQIIAWLLEKMPDLPPNNSPAP"
     gene            complement(4808..>5014)
                     /locus_tag="AC16GM_04950"
     CDS             complement(4808..>5014)
                     /locus_tag="AC16GM_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923062.1"
                     /translation="SVDASVGYFCFAVTGHYSHHDEKRDFSCDDTGETLRIPGIQLVG
                     YVSTINPPCPGVDSAQFLKKSGNQ"
BASE COUNT         1189 a         1235 c         1302 g         1288 t
ORIGIN      
        1 ccccggacga ttaccttctg atttggtact tggcacaagt tcaggtccag cgctggcagg
       61 ctaccggtaa ccggaccatg cttgagccag ttctcagagc tgtcaacggc gcgctccagt
      121 acgatccgga aagtaacgac ctccgtcggt tgcgggagtg gctccggtca cagtgaccct
      181 atgggtgcgt ggcggccagt gtctattcct ttatagggag gtacagcggt gagctccaag
      241 gctaccttac aatactttgg cagagatttg gaagccatgt catttgcgga gaactatcac
      301 aaatggatcg tggatgagtt ccggccgtat ctgggtgatt ccgttgcaga aattggtgcc
      361 gggacaggca atttttccaa atttctggtc gccgcagaaa taaaaagcct ggtcgcattt
      421 gagccatccg gaaatatgta tcctttgctc aaagagcact ttaagaacaa taagaaggtt
      481 gacacggttc ccgcctttct aaaagacgca gtaaagaatt accaagacag cttcgattcg
      541 gtcgtttacg tcaatgtcct ggaacatgta gaagatgatg agaaagaact ctcttacgtg
      601 ttcgcagcct taaaggaaaa aggccacctc cttctttttg ttcctgcatt atcctggctc
      661 tacagtaaac ttgatcggaa tctgggacac tatcgtcggt atcacaagaa tgcccttgca
      721 aaactcgttc agaacgcggg attctctatc gtaaaggtta agtactttga ctttgtgggc
      781 atcctgcctt ggtttattgc cttcgtcctg ctccagcagt caataactgg agacagcgtg
      841 gcgctctatg acaaattcgt tgttcccgta atgcgagttg ccgagggtgt aatctcccca
      901 ccgatcggga aaaatctgct gttagtcggg aaaaagaatg gccgtcgttt tggaggcggg
      961 tagcgatcga gggacagata acctttggtc ccggacgcca taccgcttca gttttgtctg
     1021 aattcattgc cggtgctgac aaaaagtgct tgacaacacc atgggtatat tcctatgttt
     1081 agcaatgata tccttctttc gggttttcct ttcacaattt cgatgtaata ccaagggatt
     1141 gcctcggtca ccctgtggac ttcagttgcc gagggggagg tgcttatgca cgtgacgaga
     1201 attgcattgg tcgccgggct gcttgcctca ttctgtttta ccagcgcggt cgcttcggac
     1261 ggcacctggc agcgcatttc cgattctccc caaccaacgg tgttcgggga cgggtatgtt
     1321 ctggatttgg gcccgctcca gccgccggtg cccacgatcc cggggtacac ggtgaccccc
     1381 ttcgccggaa tccagcgtcc ccggggtgtt tcgttcaatg ctgccggtga tctcttcacg
     1441 atggatcggg attccggaac aattttcagg atcaccccgg atggcgccgt gagcgtcgtg
     1501 gctgatttgc ctgatattct ggccggttat gtcgggccgc tcttcgaccc ggtttcggga
     1561 aatctctttg tctctcgctt cgcggtttat acgggaaacg aggtactccg gattacgcca
     1621 gccgggaatg tcgacgtcta cgccacgggc attctccctc ccgggggttt gaccagcgat
     1681 ccggcgggaa atctttacgt ttctcagttt gaagatgggg gacaggtttt caagataacc
     1741 ccggcacgtg aggtgagccc ttttgcttcg ggattgtact atccggacgg cctggtcttc
     1801 ggaccgacgg gcgacctctt catctgcaat acccgtctga atgagatcag gcgggttcct
     1861 cccaccggcg gtactgcggc tttgtttgcc tcgggattca actatcccat cgcggccacg
     1921 ttcgacaacg caggaaatct tttggttgcg gagtatctcg gcggctcaat tgccaaagtg
     1981 agtccgactg gcacggtgag cccattcggg agcgggttca tcagccctgc cggattggcc
     2041 tttgacgatc aaggggatct cttcatcgcc gattttggcg caaacatgat ttacaaggca
     2101 gtaccggggg ccgtgggcca gccgattcca gcgctattcc tgggaagaac gagcggtctg
     2161 ccaggggaca ccgttctgat gccacttcgt ctccggcctc cccatgggct ggcggccttg
     2221 gatgcgacca ttacctggaa cccggccttg gcgcagtgca ttgaagtcca caaaaccccg
     2281 gcgactgatt cattcttgct ggagagtaac ttgacggtac agggggaagc acaggtggca
     2341 atggtcagcc gtgaaccgat accgagagga gccgcagatg agctgttggt attctctttt
     2401 gtgattgaag ataccgtctc gcgagggacg accattgaac tcaacattag ggaaccccac
     2461 ttattcgacc ccaatggcga tcatataggc gtagttcttt tttatcccag tggcgaaatc
     2521 ttcgttggta acccgccggg agacgtggat ggtgatggcc gggtgacgat tgccgacgtc
     2581 atcctcacac tgcaaatcgt cgtgggaacc cacacgccta ccgagattga acggatggcg
     2641 gcggatgtca atcatgacgg tgaaatcaac tcagcggacg cggtgatcat cctccacatg
     2701 gtcacgaccg aggcagctac caggccaccg gtcgattcga gcgcggcggt gagcttcgtc
     2761 gcgccgcaag tcgcatacaa cccgtcagac cagacggtac gcctcacact gtttggagag
     2821 ttgctgactg acgttgcggg tgggaaactg cacgttcaat ttgatccgac cttagtggca
     2881 ctccagcaaa ccgcggcggg ccgggacgtt ggccagacgt tgaatgaatt tgcttctgat
     2941 gctgccaacg ttcgattcgc tttcgcaaaa tcgtcaaacc tgacgcttgc ggtcggtgaa
     3001 ctggcgcagg tgcagtggcg cgccttacaa tctttgacac ttgagcagct gaaccgagcc
     3061 gtgcgcgtcg tcggcactga gttctacgga gagaacgggc agagcattcc ggttgcggtt
     3121 cggttgagcg gcccgacggg cgtcaccaat caaaacggga cggtgcccaa gagttatgcg
     3181 ctccggcaaa acgtgcccaa tcccttcaac gcccgcaccg cgattcactt cagtctgccc
     3241 cagccgggag aggtcgcgct ggagattttc aacgtcgccg cccagaaggt gcggactttg
     3301 gtcaacgcgc attacccggc gggtgaacag gtcatcttct gggacggccg cgatgatcaa
     3361 ggagaagagc tcgccagcgg cctctacttc taccgcctga cggccggaag tttttccgat
     3421 accagaaaga tggtgttggt aaagtaagaa gaatcacggg gcaggagaat tatttggcgg
     3481 caaatcgggc atcttttcaa gaagccaggc aattatttga atcccggcat gtgatatttt
     3541 gctactcaga atttctgaac tcactttaaa gggtccgaaa tcggtcgcat ctttggaaat
     3601 tgctatgtct tcggctgacc agttcgttga ttctatgttg agatttttga ttgctacaaa
     3661 gccaattggc attgctgtca accctccact agtggtcaac tgccctttcg ctgtaattgg
     3721 aataaaccag gacttatcat taataaatgc ggtaagaatc caggggcggt caatacctac
     3781 gatgcagtaa tcaaaggaaa tagaaatgct actagtcttc acaggttgag tgggtgcatt
     3841 gtcagctaaa tacttattaa ctagcattct ttttcttata tccagcatgt tcagttgctg
     3901 aagataatta gtatgaacgg caaaactttt cacactaacg gcaggactca actcatgggc
     3961 aatatttatg tttaacttac taagggatcc agccgcaaga gttggcttgg taggctgaat
     4021 aatttcattt gattttgtat gtgctataaa catagcttgt gggatagtct ttacgcttaa
     4081 aacattcttg taaagactaa ccggatcggc gggctgtgat tcatctgaag gcagcacttt
     4141 ttgtatagca gcatcatcaa ttttcagctt ccaaagttga aatttaggac tattatcaac
     4201 cggtgctcca gttgtttcac tcgcctggaa caagtgcctt gtccataatt cgtctcgggc
     4261 tttatcgtac cagctttcag gagtagccag gcatggttta tagtccagga ttaatcctgt
     4321 ggacgattct aagacgatgt tctgccatag cttcttagca tcagctttca tctcattgaa
     4381 agaattcata atagtctctt tactttggtc atcaatacct tcaggaaaaa atggactgga
     4441 gggatttaac agatggtaaa aatagaactc atcaatctgg ttgatttctg aaaagtggat
     4501 attcaaccca tcagcttcgt ctacaggaat ttgattaaca agatcggaaa atttttcttt
     4561 agctagagca ggcaaatatc cagattcagg atggttaggg tcaataaaat catcatcgga
     4621 aattactgac ccgaatttat cgaatcggaa gaacacccgg cttccagcat cagtcttaaa
     4681 tttattaata aaaaaatcat tcaaaggctg gtgaattgta tcgagcgata acggcatctt
     4741 attccctgtt agagttgata atgcagaata gtgaagaagg cataccaaaa cgcccgcccc
     4801 taacccttca ttgattgccg gatttcttca aaaattgcgc tgagtccacc ccaggacatg
     4861 gaggatttat cgtagaaaca tatccaacga gttgaattcc tggtatgcga agtgtttcac
     4921 cggtatcatc gcaactaaaa tccctttttt cgtcgtggtg actgtaatgc ccggtaactg
     4981 caaaacagaa atagccgaca gaagcatcaa ccga
//
LOCUS       DBNGZD010000183         5068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_99328, whole genome shotgun sequence.
ACCESSION   DBNGZD010000183 DBNGZD010000000
VERSION     DBNGZD010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_99328"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="AC16GM_04955"
     CDS             complement(<1..916)
                     /locus_tag="AC16GM_04955"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2923063.1"
                     /translation="MMKKAKVLWLLTLAVLGPHLAWGQAKVGTAGAQFLELGVSARAA
                     AMGDAFVSLANDATALYYNPAGLTQLSGKQAVFSGVQLPADMQLGFVGFVLPVAKLGG
                     QMGVSVYGLNSGDIEETDYGHPSGTGRTFGVFDLAVALSYARSLTDHFSMGVTAKFIQ
                     ESFNGVGFRITPSGGQESIDEHSRGWAADVGTLYDTGFRGFRIAMSITNFGPDLKLSS
                     NEFPGEAFKRQAYALPINFKFGTAINVVELPNNRVTLALEGSHPSDNLEKFQGGVEYA
                     WHDILALRPGARARPNLTSEPKNIEALEP"
     gene            complement(946..3534)
                     /locus_tag="AC16GM_04960"
     CDS             complement(946..3534)
                     /locus_tag="AC16GM_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923064.1"
                     /translation="MRLKKEKRGSFWAGLAVSFAILSLGLAHTGWARMAIQPTPTDFS
                     LAKPTGCKTPSTLYCGHKVGKLWLMVTNFGQFGDEPDNPYFAENCFPGVNKPGKFSAQ
                     YPGGSGTEYLFTGALWCGAVSGKDTLTTTAFDGWLNVSEMFADVAPQGDFTARSINPG
                     SPFYSPDAISTFDLIAAYTDTMTCSGFVNADQYSGRGHKPLNIKVIQKSYSWDYSYAK
                     DFVLFDYQVINMGKIPLESFWMGIYIDADVWPSRLSSVGFKDDLAGFRQYALDPATGD
                     SVINVAYIGDNDGDGEGGVFKDKRNTKAVTGTRVVRTPNPDLKFSFNWWISEGTNPVD
                     QDWGPQTLAKKRQNYSGGWGTPEGDIIKYWYMSNNEFDYDQIQLELEHRDWIPPPKNA
                     DSLVDGFDTRYLLSFGPFDVNPGDTLPITLAYVAGDDWHRNANDWKNTFPAGDFSKFK
                     DSAVVNNFINNTLNWDDLARNAQWAGKVYDNPICVDTIINGQPKRICKGDGIPDFKGP
                     PPPTPPALRLDADVGKITVRWNGKETEAKAPDPFSNVLDFEGYRIYLSRSAVAGDFVL
                     LGSYDFIDWQVWYKNEGLSPAVWQRLKTEKRPLTLPEIKQQFGATVDPEVVKDSSVAI
                     RTHRGTGTRDTSFFFRPEDYNRGLDPIRVYRDTAAARRDFPTLSPAALDSLFNDSLKY
                     EYQYSADHLIPSRPIFVGVSAFDFGDVASGLDPLESSPQSNAKFAFPLATIDRVFAAK
                     EKVKVFPNPYKIDAGYGIFEQPQGIPEQATEYDRKIHFINLPPKCTIRIFTVDGDLVR
                     EIQHDYSSRPFDPLASYDVWDVISRNTQSVVTGLYLYSIEAQDVPAFPIPPELKRQVG
                     KLVIMK"
     gene            complement(3700..>5068)
                     /locus_tag="AC16GM_04965"
     CDS             complement(3700..>5068)
                     /locus_tag="AC16GM_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2923065.1"
                     /translation="DPGTFEIRGIYEQRTVTLTTAAFDITSQFKKSHEVKTGLKLEKN
                     RLTMQDIRYVEVPYDGPPDPTQPFNDRGIFRDFYDRSPIRLATYVQDKIEYGALIARL
                     GLRYERFLQANLNDLRKEREDAGLIDTVIARNRSKLAPRMGFSYPITERAKIYFNYGH
                     FYQLPEFQYFYRRSTQGSLAFGIIGNFNLDFSKQISYEFGVNIALSNSYKLDVSGFFK
                     DYFGQINTLVQRNGPIERDIYANLDYARTRGFELELDKKYGNYISGYVNYQFAYAFGK
                     ASTESQNYNDRFNNRQIPIKENPLSWDQRHQITFNLDLRIPRKERPRIFGLRVPDNWG
                     INVVWQYGSGFPFTPTRTFPGLRVPPGEQPQVNSMRIPSSSQVDFRFNKDFEVWKLNY
                     SFEVWVNNVFNRQNISSVYTNTGFADTGNQPSSSAIEPGTDYQRRPDYFGPGRNVRLG
                     LALNF"
BASE COUNT         1042 a         1425 c         1490 g         1111 t
ORIGIN      
        1 gcggttctaa tgcttctatg ttttttggtt cgctcgtaag gttggggcgg gcccgcgcgc
       61 cggggcgcaa ggccagtata tcgtgccacg cgtactccac gccgccctga aacttctcta
      121 agttatcgga agggtggctc ccctccaggg ccagggtcac ccggttgttg ggaagttcca
      181 ccacgttgat ggcggtgccg aatttgaagt tgatcggcaa ggcgtaggcc tggcgcttga
      241 atgcctcgcc cgggaactcg ttgctggaga gtttgaggtc gggaccgaag ttggtgatgc
      301 tcatggcaat ccggaaaccc cggaagccgg tgtcgtacag agttcccacg tcggcggccc
      361 agccgcgtga atgttcgtca attgattcct gcccgccgct gggggtgatc cgaaagccga
      421 cgccgttaaa cgactcctgg ataaacttgg cggtgacccc catgctgaag tggtcggtca
      481 agctccgggc gtaactcaaa gccaccgcca gatcgaacac cccaaaagtt ctgcccgtgc
      541 cggaggggtg cccgtagtcg gtctcttcaa tgtccccgga gttgagcccg tagaccgaaa
      601 cacccatctg cccgcccagt ttcgccaccg ggagcacaaa cccgacgaag cccaattgca
      661 tgtcggccgg caactgcacc ccgctgaaca ccgcctgttt tcccgaaagc tgggtcagcc
      721 ccgccggatt gtagtagagc gcggtggcgt cattggcgag actgacgaaa gcatctccca
      781 tcgccgcggc gcgtgccgat accccgagtt ccaagaactg agcacccgca gtgcccacct
      841 tggcctggcc ccaggccagg tgcggtccca aaacggccag ggtcaatagc cacagtacct
      901 tcgctttctt catcatatcg gttcgctcct gtaagaatag ggcccctatt tcataattac
      961 cagtttgccg acctgccgtt tcagctcggg aggaatcggg aaagcgggca catcctgagc
     1021 ttcaatgctg tagaggtaca gcccggtgac caccgactgc gtgttccggc tgatcacgtc
     1081 ccagacgtcg taggaggcca gggggtcgaa gggacggctg ctgtagtcgt gctgaatctc
     1141 ccgcaccagg tcgccatcca ctgtgaaaat acggatggtg cacttggggg gcaggttgat
     1201 gaagtgaatt ttgcggtcgt attcggtggc ctgttcggga atgccctggg gctgctcgaa
     1261 aatcccgtac cccgcatcga tcttgtacgg gttgggaaat accttgactt tctctttggc
     1321 ggcaaagacc cggtctatgg tcgcgagcgg gaacgcgaac ttggcgtttg actgcggcga
     1381 cgattccaga ggatccagtc cggacgcaac gtcaccaaag tcaaaagcgc tgacccccac
     1441 gaaaatcggt cggctgggga ttaggtgatc cgcagaatac tggtattcgt atttcaggga
     1501 gtcgttgaac aagctgtcga gcgccgccgg tgaaagcgtc gggaagtccc ggcgcgccgc
     1561 cgccgtgtcc cggtagacgc gaatcggatc caacccccgg ttgtagtcct ccggccggaa
     1621 gaaaaacgaa gtgtcccgtg taccggtccc ccggtgcgtc cggatggcca ccgacgaatc
     1681 tttgaccact tccgggtcaa ccgtggcccc gaactgctgt ttgatctccg gcagggtaag
     1741 cggccgcttt tccgttttca gccgttgcca gaccgccggg gaaagccctt cgtttttgta
     1801 ccagacctgc cagtcgatga agtcgtacga gcccaaaagc acaaagtcgc ccgccaccgc
     1861 ggagcgggaa aggtagatcc ggtacccttc aaaatcaagc acgttcgaga acggatccgg
     1921 cgctttggcc tccgtctcct tgccgttcca gcgcaccgtg atcttcccca cgtcggcatc
     1981 caaacgcaac gccggcgggg tgggcggcgg cggacccttg aagtcgggaa tgccgtcgcc
     2041 tttgcagatc cgcttcggct gaccatttat aatcgtgtcc acgcagatcg gattgtcata
     2101 cactttaccg gcccactgtg cgttccgcgc taagtcgtcc cagttgagcg tattgttaat
     2161 gaagttattc acgaccgcag aatccttgaa cttgctgaag tctcccgcgg ggaaggtatt
     2221 cttccagtcg ttggcattgc ggtgccaatc gtcccccgca acgtaggcga gcgttatggg
     2281 gagcgtgtcg cccgggttga catcaaaggg gccaaaggaa aggaggtaac gggtgtcaaa
     2341 tccgtccact aacgagtcag cattcttggg cggcggtatc cagtcgcgat gctccagttc
     2401 caactgaatc tggtcgtaat cgaattcgtt attcgacatg taccagtatt tgatgatgtc
     2461 accttccggc gtaccccagc cgccggagta gttctgcctt ttcttggcca aagtctgggg
     2521 cccccagtcc tggtccacgg gattcgtccc ttccgaaatc caccagttga acgaaaattt
     2581 caggtcggga ttgggcgtcc ggaccacccg ggtgccggtc accgctttgg tgttccgttt
     2641 gtctttgaag actccgccct cgccgtcccc gtcattatcc ccgatgtagg ccacgttgat
     2701 aacgctgtcg cctgtggccg ggtcaagtgc gtattgccgg aagccggcca ggtcgtcttt
     2761 gaaaccaaca ctcgacagcc gggagggcca gacatcggcg tcaatgtaaa tacccatcca
     2821 gaaactctcc agggggatct tccccatatt gattacctgg tagtcgaaaa ggacaaaatc
     2881 tttggcgtag gagtaatccc aggagtaact cttctgaatt accttaatgt tgagcggttt
     2941 gtggcctcgc ccgctgtact ggtcggcatt gacgaaaccg gagcaggtca tcgtatcggt
     3001 gtaggcagcg attaaatcga aagtggaaat ggcatccggc gagtaaaaag gagaaccggg
     3061 gttgatcgac ctggcagtga agtctccctg gggggcgaca tcggcgaaca tttcggacac
     3121 attgagccag ccgtcaaatg cggtggtggt cagtgtatcc ttgccagaaa ctgccccaca
     3181 ccagagtgcg ccggtaaaga ggtattctgt gcctgaaccg ccggggtact gcgccgaaaa
     3241 ctttcccggt ttatttaccc cgggaaaaca attctccgca aaataggggt tgtccggttc
     3301 gtctccgaac tgcccaaagt tggtgaccat caaccacaat ttcccgacct tgtggccaca
     3361 gtagagcgta gagggtgtct tgcagccggt cggtttggcc agcgaaaaat cggtcggcgt
     3421 cggctgaatg gccatccgtg cccagcccgt atgggccaat cctaaagaca gaatcgcaaa
     3481 actgaccgcc agcccggccc aaaaactacc gcgcttttct tttttcaatc gcatggaaaa
     3541 agcctcctca tcttgaactc tgaaataacc tgttttccgc ccgcattgtt tcgccttttt
     3601 gtaacagtgc ctttcactca aagaaaccct gctttggctc ggtagcctag ccctttatgg
     3661 gcgtccaagg ccgacaagcg gctatgttac aaaacccttt tagaagttca acgccaatcc
     3721 cagccggaca ttccggcccg gaccgaagta atcaggccgc ctctggtaat cggtgcccgg
     3781 ttcaatcgcc gaactcgacg gctggttacc ggtatcggca aagccggtgt tggtgtaaac
     3841 agagctgatg ttctgccggt tgaagacgtt attgacccac acttcaaacg aatagttcag
     3901 cttccagact tcgaagtcct tgttgaagcg gaaatcgacc tggctggaag aaggaatccg
     3961 catggagttg acctgcggct gttcgccggg cggcactcgc agcccgggga aggtgcgcgt
     4021 gggagtaaag gggaaaccgg agccgtactg ccagaccacg ttgatgcccc agttgtccgg
     4081 cacccggagg ccgaagatgc gcgggcgctc cttccggggg atccgcaaat ccaaattgaa
     4141 cgttatctgg tgcctctggt cccaggagag cgggttctcc ttgatcggga tttgacgatt
     4201 gttaaagcgg tcgttgtaat tctgcgattc cgtggaggcc ttgccgaagg cataagcgaa
     4261 ctggtagttg acgtagccgg agatgtagtt gccgtacttc ttatccagtt cgagttcaaa
     4321 gccccgggtc cgggcgtagt ctaagttggc gtagatgtcc cgctcgatgg ggccgttgcg
     4381 ctggactaag gtattgatct gcccgaagta gtctttgaag aagccggaaa cgtccagttt
     4441 gtaactgttg gagagggcga tgttgacgcc gaactcgtag gatatctgct tggagaagtc
     4501 gagattgaag ttcccgatga tgccgaaggc taaagacccc tgggtggacc gccggtagaa
     4561 gtactggaac tccggcagct ggtagaagtg tccgtagttg aagtagattt tggcccgctc
     4621 ggtaatgggg taggaaaaac ccatccgcgg ggccagtttg gaacggttgc gcgcaatgac
     4681 ggtgtcaatc aagccggcat cctcgcgctc tttccgcagg tcgttcaggt tggcctgcag
     4741 gaaccgttcg tagcgcagcc ccagtcgggc gatcaaggcg ccgtattcga ttttgtcctg
     4801 tacgtaggtg gccaaacgga tcggactccg gtcgtagaag tcccggaaaa taccccggtc
     4861 gttgaagggc tgggtggggt cgggcggccc gtcgtagggc acctccacgt agcggatgtc
     4921 ctgcatggtc agccggttct tctccagctt gaggccggtt ttcacttcgt gcgatttctt
     4981 gaactgcgag gtgatgtcga aagcggccgt ggtcaaagtg acggtccgct gctcgtaaat
     5041 cccgcggatt tcaaacgttc ccgggtca
//
LOCUS       DBNGZD010000184         3612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1419186, whole genome shotgun sequence.
ACCESSION   DBNGZD010000184 DBNGZD010000000
VERSION     DBNGZD010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1419186"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC16GM_04970"
     CDS             <1..909
                     /locus_tag="AC16GM_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR2923066.1"
                     /translation="EKNLGGVILFARNVASLERIAAAIRQLQSRATSPLFIMIDQEGG
                     ATNRIKENFPTFPSNRSFGSRRDVEGIANAYGTTARHLKQIGVNVNLVPVVDVVTNPE
                     NKLLSERSFGSDVFLVVNCAAKAVQAIQAEGVLACAKHFPGLGDVAIDPHRELPTNPN
                     PVERFETIDFPPFRAAIEAGVASVMTTHLLCPALDPDHATTTSEIIVEKFLRDRLGFS
                     GLVLSDDMEMGAIARTGDVPRACLEAFLAGHDQILICHSAEIQEQTLALFTKELKKNS
                     QMQKRFAESLERIQTTKRKWLQHAPA"
     gene            896..2095
                     /locus_tag="AC16GM_04975"
     CDS             896..2095
                     /locus_tag="AC16GM_04975"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR2923067.1"
                     /translation="MPRPNPGLLTVARQRTRVVIGLNSGTSANSIDACLVEIKGQGLK
                     RSVRCLAFRSFLFSPVEQGTIFRFSQPGVVDTETLARFSQKLGRQFARAAQKMIALAR
                     RRGKKVDLIGSHGQTVGHFPEPGSASGIAERATLQIGEPEIIAKTTGVVTVADFRPAD
                     IAAGGQGAPLSPVAHWALVRSRKKNRVILNIGGIANLTILPKAADLTKIWGFDTGPGN
                     MVIDGLMQRLFGKSRDGGGITAARGKVSKELLMRLKAHLYFHRKPPKTTGREMFGQEF
                     VERCLSWRRKLKVSNADLIATVTELTAWSVWDACQKFIRPRLKIDELFVSGGGARNRH
                     LLQRLGFYFSPVPVKSLTALGLDPQALEAILFALLADFCVTGTSLPLRRITGAKKPVV
                     LGKICQP"
     gene            2281..3459
                     /locus_tag="AC16GM_04980"
     CDS             2281..3459
                     /locus_tag="AC16GM_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR2923068.1"
                     /translation="MPGRVEVRVHLFSTTSPFRVECYRGLKAKRTRTTDSASSGAVSR
                     YPDFGDALSWSSSEFYLDPQTAAVTLTPKGSPATVTIGEKLYRGRLEITKDLWNGLSV
                     VNVLEIEDYLKGVLPPEIGNRAENEFEAVKAQAVAARTYTLSKLNQYPGKNYDLEADT
                     RDQVYGGVTIETDLTNRAIRKTHGRVALYGGRLINAYYHANCGGCTDSISRIWAKPQE
                     DYLIPVEDSDFCRWSQTRLSEPNNYRWEERFPRSLLEHNFKTFLPPAYGKDTTGLLFI
                     DGLAVKSRTTGGRVDSLLVIWNAARGFDSTVLVRDSIRWAFRRASDTTRILRSTKFEV
                     SYVMDSSGLIDTVVVTGVGTGHGVGLCQTGAIELARRGYSYEKILQHYYPGIKIRRIY
                     "
BASE COUNT          771 a         1142 c          944 g          755 t
ORIGIN      
        1 gaaaaaaacc tgggcggggt catccttttc gcccggaacg ttgcctctct ggaacgaatt
       61 gccgccgcca ttcgtcagct gcagtcacgc gcaacttccc cccttttcat tatgatcgac
      121 caggagggcg gcgccaccaa ccggatcaag gagaatttcc cgacctttcc ttcgaaccgc
      181 tccttcggca gccgccgcga tgtcgaaggc atcgccaacg cctatggcac gaccgcccgc
      241 cacctgaagc aaatcggcgt caacgtgaac ctagttcccg tggtcgatgt ggtcaccaat
      301 ccggaaaaca aactactttc ggagcgctcc tttggctccg acgtttttct ggttgtgaac
      361 tgcgccgcca aagccgtcca agccattcaa gccgaaggcg ttttggcctg tgccaagcac
      421 ttccccggcc tgggcgatgt tgccatagac ccgcaccggg aactgccgac caatcccaac
      481 cccgttgagc gttttgaaac gattgacttc ccccccttcc gggccgctat cgaggccggc
      541 gtcgcgtcgg ttatgacgac tcacctcctt tgcccggcgc tggatccgga tcatgccacc
      601 actacgtccg agatcatcgt ggagaaattt ttgcgcgatc gcctgggctt ttccggtctc
      661 gttcttagcg acgatatgga aatgggcgcc attgcccgga cgggagacgt cccccgcgcc
      721 tgcctggagg ccttcctcgc cggtcatgac cagatcctca tctgtcattc tgccgagata
      781 caggaacaaa cgctcgctct cttcacaaag gaattgaaaa aaaattccca aatgcaaaaa
      841 cggttcgccg aatcgctgga gcgtattcag acaaccaaaa ggaaatggct gcagcatgcc
      901 ccggcctaat cccggtctat tgaccgtcgc tcggcagcga acccgcgtgg tgattgggct
      961 gaactccggc acttccgcca acagcatcga tgcgtgtctg gtcgaaatca aaggccaagg
     1021 cctcaagcgc agcgtaaggt gcctggcttt tcgctctttc cttttttccc ctgtggaaca
     1081 ggggaccatt ttccgcttct cgcagcccgg cgtggttgat accgaaacgc tggcccgttt
     1141 ttcccaaaag ctcggacgtc aatttgcccg cgccgcgcag aaaatgattg ctctggctcg
     1201 ccgccgtggg aaaaaagtcg atttgatcgg ctcccacggc cagaccgtgg gccacttccc
     1261 tgaacccggg agcgcttcag gcattgctga acgcgccacg ttgcaaatcg gcgagccgga
     1321 aatcatagcc aaaacaaccg gagtggtgac cgtcgccgac ttccgtccag ccgacatcgc
     1381 cgcgggcggg cagggcgcgc ccttaagtcc ggtggcccac tgggcgctcg ttcggagcag
     1441 aaagaagaat cgggtcatct tgaacatcgg cggcatcgcc aatctgacca tcctgccgaa
     1501 agctgctgac ttgaccaaaa tctggggctt cgacaccggc cccggcaata tggtcattga
     1561 cggtttgatg caacgcctct ttgggaaatc tcgcgacggc ggaggaataa ccgccgcacg
     1621 cggaaaggta tcaaaagagc tattgatgcg tctcaaagca cacctctatt ttcaccggaa
     1681 accgcccaaa accaccggac gagaaatgtt cgggcaagaa tttgttgaac gctgcttatc
     1741 ctggcgcagg aaattgaaag tctccaatgc ggatctcatc gccaccgtca ctgaacttac
     1801 cgcttggtca gtctgggacg cctgccaaaa attcatccgc ccgcgactga aaattgacga
     1861 gcttttcgtc tccggcggcg gcgcccgtaa ccggcacttg ctgcaacggc tcggtttcta
     1921 cttctccccc gtaccggtga aaagtcttac tgctctggga ttagatcctc aagccctcga
     1981 ggccatcctc tttgctctac tcgccgattt ctgcgtaacc ggcacgtccc tgccgctccg
     2041 tagaatcacc ggcgcaaaaa aacctgtagt tttgggaaaa atctgccagc cgtgatgagg
     2101 acaatcctct taaaaaagcg tggagttaaa tcactctttt cattattcat tgccttaaat
     2161 ttgttggcgg gttgtgctcg gcgcttgccg ccgcccccgc cgccaacacc aacacctccg
     2221 ggggaagtcc cgcctgcacc ccaaccgcaa ccgcaggccg tgcctttacc tccgccgcct
     2281 ttgcccggtc gcgtcgaggt tcgcgtccat ctcttttcca ccaccagccc cttccgcgtt
     2341 gaatgctacc ggggcttgaa agcaaaacga acccgcacca ccgactccgc ttcgagcgga
     2401 gcggtttccc gctatcctga ctttggtgat gctttgagct ggtcgtcgtc tgaattctac
     2461 ctcgatccgc agaccgccgc agtgacgctt acccccaaag gcagtcccgc taccgtcacg
     2521 atcggcgaaa aactctaccg cggccggctg gagattacca aagacctgtg gaatggtttg
     2581 agcgtggtga atgttctcga gattgaggat tacctgaagg gcgtcctgcc gccggaaatc
     2641 ggcaaccgcg ccgaaaacga attcgaagcg gtcaaagccc aggcggtcgc cgcccgcacc
     2701 tatacgctct caaaattgaa ccagtatccg ggaaagaatt atgatctcga agccgacacc
     2761 cgcgaccagg tgtacggcgg cgtgactatc gaaaccgatc tgaccaatcg ggcgatcaga
     2821 aaaacccacg gccgcgtggc gctctacggg ggacggctga tcaacgccta ctaccacgcc
     2881 aactgcggcg ggtgtaccga ttctatctcc cgcatctggg ccaaaccgca ggaggactac
     2941 ctcattccag ttgaggatag cgacttctgc cgctggtcgc agactcgact ctccgagccg
     3001 aacaattacc gctgggaaga gcgcttcccg cgctcccttt tagagcacaa cttcaagact
     3061 ttcctgcccc cggcttacgg gaaagacacg accggcttgc tctttatcga tggcctcgcg
     3121 gtcaaaagcc gcaccaccgg cggtcgggtc gactcgcttc tggtcatttg gaacgccgcg
     3181 cggggatttg actcgactgt cctagtgcgc gactccatac gctgggcttt ccgccgcgcc
     3241 tcggacacca ctcgcattct gcgcagcacg aagttcgagg tgagttatgt gatggactcc
     3301 agcggcctta tcgatactgt agtcgtcacc ggcgtcggta ccggccacgg ggtggggctg
     3361 tgccagaccg gcgccatcga actggctcgg cgggggtact cgtacgagaa gattttacaa
     3421 cactactacc ccgggattaa aatccgcaga atttactaac tgatttttct attcacggat
     3481 acctgattta aatcccaact ttcacagaca acacggctgc ggcgtcccga aggtgacaaa
     3541 ttggatgagc aggacaacgt cgctaatatc ccgtacccca tcgcagttgg tatctcctgc
     3601 cagcgggtta gg
//
LOCUS       DBNGZD010000185         2406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1627705, whole genome shotgun sequence.
ACCESSION   DBNGZD010000185 DBNGZD010000000
VERSION     DBNGZD010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1627705"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="AC16GM_04985"
     CDS             complement(<1..1459)
                     /locus_tag="AC16GM_04985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR2923069.1"
                     /translation="MPDADFGVTARDVPRFFESGRKTIALLIGGAISVFLPFGIYLVT
                     LAAVVGFTDSGELAVVSQTLGIAHPTGYPLYTLVTHLFTWLPVGSTISRTGLFSALVA
                     SATNGLLYLCVLELFAMLGAGLRSNFRSVAIATGTALSLAFTALFWTQGNTNEVYALH
                     LFFISLATLQFLKWQKTVREEGVGTPDAQRLFLLLAFASGLGFTNHMTIGVLMPALLL
                     GGWATVKRIPYRAPWFWVGLGLFLGALTLYLYLPVRSAQNPLFDWGRPTNLGRFFRHV
                     SAWQYRVWMFSGPESQIKENFSHFIALFRTEYLWFWLLPAGIGLVTLFRKNWPVGLFL
                     LLALAGNFSYNLNYHIPDIDSYFLPFVFWYLLLGGVGIAAIISWLLGKHALVLRGIGW
                     LGLAGTIFFPARAVLQNYPKANQKTYLFAYDYAQNFANGLLPDAVVLTSLWDHYAPWL
                     YLHYLEGRRTDVTFIDVELCRRSWYFAYLARADSTL"
     gene            complement(1496..1909)
                     /locus_tag="AC16GM_04990"
     CDS             complement(1496..1909)
                     /locus_tag="AC16GM_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR2923070.1"
                     /translation="MASVNKVILIGNLGRDPELRYTPGGQPVATFTLATTDRWKDKEG
                     QLQDRTDWHNIVVWGKTAENTKEYLKKGRQVYVEGRIQTRSYDDRDGNKRYITEIVAQ
                     RVQFLGRREESAAGGPAVAEAEPEPQAAVDEDVPF"
     gene            2102..>2406
                     /locus_tag="AC16GM_04995"
     CDS             2102..>2406
                     /locus_tag="AC16GM_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR2923071.1"
                     /translation="MALAQIAPKLGDVQANLKTHLALIRKARARKADLIIFPELSLSG
                     YLLKDMVPRVALKLDAPEVKHVLTESKSFAMAVGLVEETPESLYYNSALFLEKGKMV"
BASE COUNT          575 a          632 c          761 g          438 t
ORIGIN      
        1 agagggtcga atcggcgcgc gccagatagg cgaagtacca ggagcggcgg cagagttcga
       61 catcgatgaa ggtaacatcc gtgcggcggc cttcaaggta atggagatag agccagggag
      121 catagtggtc ccagaggctg gtcaacacca cggcatcggg gagcagaccg ttggcgaaat
      181 tctgggcata gtcgtaggcg aagaggtagg ttttttggtt tgccttggga taattctgca
      241 ggacggcacg cgccgggaaa aagatggtgc cggccaggcc gagccaacct ataccccgta
      301 gcacaagggc atgtttgccc aaaagccagc tgatgatggc ggcgattccc acaccgccga
      361 gcaagaggta ccagaaaaca aagggcagga agtaggaatc gatatcgggg atgtggtagt
      421 ttagattgta ggagaaattg ccggccaaag ccaataagag gaaaagtccc accggccagt
      481 ttttccggaa gagcgtgacc aggccgatcc cggcaggcaa aagccagaac cacaggtatt
      541 cggtgcggaa cagggcgata aagtgggaaa agttctcctt tatctgtgac tccgggccgg
      601 agaacatcca gacgcggtat tgccaggcgg aaacgtggcg aaaaaatctt ccaaggtttg
      661 tcggacggcc ccagtcgaag agcgggttct gggcggagcg gacgggtagg tagagataca
      721 aagttaatgc tcccaggaac aagcccaatc cgacccagaa ccagggagct cggtaaggaa
      781 tgcgcttcac ggttgcccag ccgccgagca gcaaggccgg catcagtacc ccaatggtca
      841 tatggttcgt gaagcccagg ccagaggcaa aggccagaag caggaagagc cgctgagcgt
      901 caggcgttcc cacaccctcc tcccggacag ttttttgcca cttgagaaat tgaagggtgg
      961 cgagagaaat gaaaaagagg tggagggcat acacctcgtt ggtattccct tgcgtccaaa
     1021 agagggcggt gaaagccagg gaaagggcag tcccggtcgc aatcgcgact gagcggaagt
     1081 ttgagcgcaa gcccgccccc aacatcgcaa aaagttccag aacgcaaagg tagagaagcc
     1141 cgttggtcgc agatgcgacc agagccgaga aaaggcccgt gcggctgatg gtgctcccga
     1201 ccggaagcca ggtgaacaga tgggtgacga gcgtgtagag ggggtagccg gtgggatggg
     1261 cgatgcccag agtttggctg actaccgcca gttcacccga atcggtgaag ccgaccacgg
     1321 cggccagagt tacaaggtaa ataccgaacg ggagaaaaac ggaaatcgca cccccgatca
     1381 gaagtgcgat ggtctttcgg ccgctttcga aaaaacgcgg cacgtccctg gccgtaaccc
     1441 caaaatctgc gtcgggcaag accgcctctg gtcgatatgg ctcataccga gccgattaaa
     1501 agggaacgtc ctcgtctact gcggcttgcg gttcgggctc agcttcggca actgccggtc
     1561 cgcccgcggc ggattcttca cgccggccga gaaattgcac gcgctgggcg acgatctcgg
     1621 tgatgtagcg cttgttgccg tcgcggtcgt cataggagcg ggtctggatg cggccttcga
     1681 catagacctg acggcccttc ttgaggtact ctttggtatt ctccgcagtt ttgccccaga
     1741 cgacgatgtt gtgccagtcg gtgcggtcct gcagctgacc ctccttgtcc ttccagcggt
     1801 cggtggtcgc cagagtgaag gtggcgacgg gctggccgcc gggggtgtag cgcagttcgg
     1861 gatcgcgccc gaggttgccg atcagaatga ccttattgac gctcgccata gggactcctt
     1921 tacgtcaggt cgtttgtagc caaataaaat agggggctga cccgctggcg tcaatcctgc
     1981 cggaagggat cggcgactcg atctcttaac cttgcgaaaa cctttggggg gtcatatctt
     2041 ttttcgttca atgccgaaac gagttatccg agaatcaggt acgccaaaaa acaggctgcg
     2101 ggtggcgctg gcgcaaatcg ctccgaaatt gggggacgtg caggccaatc tcaaaaccca
     2161 cctcgcgctc atccgcaaag cccgggcgcg gaaggcagac ctcatcattt tccccgaact
     2221 ctccttaagc ggctacctgc tgaaggatat ggtgccccgg gtggcgctga aactcgacgc
     2281 accggaagtc aagcatgttc tgacggagag caagtcgttt gccatggcgg tcggcctggt
     2341 agaggaaacg ccggagtcgc tctattacaa cagcgccctt tttctggaaa aaggcaaaat
     2401 ggttta
//
LOCUS       DBNGZD010000186         4997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1002393, whole genome shotgun sequence.
ACCESSION   DBNGZD010000186 DBNGZD010000000
VERSION     DBNGZD010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1002393"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /gene="tyrS"
                     /locus_tag="AC16GM_05000"
     CDS             complement(<1..1163)
                     /gene="tyrS"
                     /locus_tag="AC16GM_05000"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR2923072.1"
                     /translation="MKFPSIEEQLAVIKQGAVEVIPETELVTKLKRAQATGKPLRVKQ
                     GFDPTAPDIHLGHTVGLRKLRQFQELGHRIVLIIGDYTGMVGDPSGRSATRPQLSFGA
                     VRRNAGTYLKQFAKVLDREKVEIHYNGEWFSKMSFQEIMALAGKFTLARMLERDDFSI
                     RYKEQSPIAIHELFYPLMQAYDSVAIRADVEIGATEQKFNLLAGRQIQEEYGQEPQIV
                     LTLPVLVGTDGVQRMSKTTGNYIGVAEPPDQVYGKAMSIPDEQILPYLELVTDCSPTE
                     LTQAREELRKRSVNPMLWKKKLAHRLVTLYHGKGAADRAQAEFEQVFSEKKLPEQIPG
                     VALSYRNGKIPIVQLLMETKQADSKSAARRLIQQGGVSVNQKRVADLDFEVEL"
     gene            1260..3392
                     /locus_tag="AC16GM_05005"
     CDS             1260..3392
                     /locus_tag="AC16GM_05005"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR2923073.1"
                     /translation="MTLFIRYMDESKFAVEKYPATAPYPLPPRRRRRVSVFLGYLLGS
                     LTLAGLVAALLTYRVYKHDLPSLAQLTNLQPSLATKVYARTGRVLKEFYTERRIFVPL
                     GQMPPYLIDALLATEDRKFYNHWGVDPLGVARALFSNLSRGELVGQGGSTVTQQLART
                     LFLTRQKTLSRKIKEILTAIKIERAYSKREILELYLNQCYFGKGAYGIQAAAQTFFSR
                     SAPELTLPQCALLIGLLQAPNRYSPLEHPERTLARRNIVLDAMVDYKKLNANFADSLK
                     RLPLELSPNVDVLGEAPYFTEMVRQYLEDRYGEKMLFSGGLMIQTTVDDSLQKIAEEA
                     VRSVIDSLQKDLERSHTLSDPRYTEEVVDTVNGKIEKRRQYKQIQGAFLAIDNATGDV
                     ITMVGGRDFNQSKFNRAVQALRQPGSAFKPFVYTAAVDNGYRPCDLLFDSPLVLTIPG
                     SGEWRPQNIDFSFRGPMTLREGLQFSRNLIAIKLLQKIQPQQAIYYAKKMGITSPLQP
                     VPSLAIGTSEVSMWDMVSAYSVFPNGGIRIEPKYILRITDRFGNILEKHETSKREEAL
                     SPTTAYIMVNLLQTVVDRGTGARTRAMGFTRPAGGKTGTTDEFTDNWFIGFTPQFTAA
                     AWVGFDDKTSIGYNQTGSSNALPIWTKFMLAAHENLPVKDFTVPPGIVFANVCKESGE
                     LAKDDCPEILYNEVFTEATVPKERCHIH"
     gene            complement(3810..4448)
                     /locus_tag="AC16GM_05010"
     CDS             complement(3810..4448)
                     /locus_tag="AC16GM_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923074.1"
                     /translation="MKALKKQGRSFVKSQFFAKFLPVAVLILVLGLVGASAYWYEHNR
                     LGKAVEVVVLTLTLVAVAFYVYLTYILALASWIPVGTFHMKQVEKDPYHILTFPGNPT
                     KQQNIKMWVKLNATIYGQPVELGGFYSAESPWYLQPLSQGQGHFRIETLLEKVGQTIA
                     AMENSATPTNVREQLRFSVNIRFEGVESQIQREYPVQNYYFDFSAKVLVLDV"
     gene            complement(4594..>4997)
                     /gene="deoA"
                     /locus_tag="AC16GM_05015"
     CDS             complement(4594..>4997)
                     /gene="deoA"
                     /locus_tag="AC16GM_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019448.7"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIR2923075.1"
                     /translation="TGAALAKFREMVKRQGGDERIVDDYSRLPQARYRISVPSAKSGW
                     VKAIDTYRVGMLAVELGAGRKQITDPIDHAVGFLIHKKVGQKVAAGEALATVLANDEE
                     QGREVAQQLQQCFSLAGRAVRPLRKILGELR"
BASE COUNT         1040 a         1472 c         1370 g         1115 t
ORIGIN      
        1 aaaagctcca cctcaaaatc taagtctgcc acccgcttct ggttcacgga gactccgccc
       61 tgctggatga gccggcgggc cgcgctcttc gagtccgcct gcttcgtttc catgagcaac
      121 tggacgatgg gtatcttccc attccgatac gagagagcca caccggggat ttgttccggc
      181 aatttctttt cagaaaagac ttgttcaaac tcggcctgcg cgcggtcggc ggcccctttc
      241 ccgtggtaca gggtaaccag ccggtgcgcc agtttcttct tccagagcat cggattcacc
      301 gagcgctttc ttaattcctc ccgcgcctgg gtcagttcag tcggactgca atcggtcacc
      361 aattctaaat agggtaaaat ttgctcgtcg ggtatcgaca tcgccttgcc gtaaacctga
      421 tccggcggtt cagcgacgcc gatgtagttc cccgtggtct tgctcatccg ctggacgccg
      481 tcggtaccca ccaagaccgg cagggtgagc acgatctggg gctcctggcc gtactcctcc
      541 tggatttgcc ggccggccaa aaggttgaac ttctgctcgg tggcgccgat ttccacgtcg
      601 gctcggattg ccaccgaatc gtaggcctgc atcaggggat agaacagttc gtggatggct
      661 atggggctct gctctttgta gcggatcgag aaatcgtccc gttccagcat ccgggccagg
      721 gtgaatttgc cggccagggc catgatttcc tgaaaactca ttttgctgaa ccattcgccg
      781 ttgtagtgaa tttcgacttt ttcccggtcg agtaccttcg caaactgttt taagtacgtc
      841 ccggcattgc gccgtactgc gccaaaagaa agctgcggcc gggtggcgga gcggccggac
      901 ggatcgccca ccatgccggt gtagtcgccg atgatgagca cgatccggtg gcccagttcc
      961 tggaactggc gcagcttgcg caatcccacc gtatggccca ggtggatgtc gggagcggtg
     1021 ggatcaaatc cctgcttgac tcgcaggggt tttcccgtcg cctgagcacg cttgagtttg
     1081 gtcaccaatt ccgtttccgg aatcacctcc accgcgccct gcttgatgac ggctaattgt
     1141 tcttcaatgg atggaaattt catccgacat aggataatag tcggaaggga aaaaggcaag
     1201 tgcctgcgac gcatccggca cgctgtttcc tgagagaaaa ccttgacagt cgcctgcaaa
     1261 tgaccctttt tatcaggtat atggacgagt cgaagtttgc cgtggaaaaa tatccggcga
     1321 ccgcgcctta tcccctgccg ccccgccggc gccgccgcgt atcggttttt ctcgggtacc
     1381 tcttgggttc tttgaccctg gccgggctgg tagcggcatt gcttacctac cgggtctaca
     1441 agcacgatct cccttcgctc gcgcagctga ccaacctcca gccctcgctg gcgaccaaag
     1501 tctacgcccg tacgggccgg gtgctcaagg aattttacac cgaacgccgg attttcgtcc
     1561 cgctggggca gatgccgccc tatttgatcg atgcgctctt agccacggag gaccgaaaat
     1621 tctacaacca ctggggtgtc gatcctttgg gagtggcgcg cgccttgttc tccaaccttt
     1681 cccgcggcga actggtcggc cagggcggct ccaccgtgac ccagcagctg gcccgcaccc
     1741 tctttttgac ccgccagaag accctttcgc ggaagatcaa agaaatcctg accgccatca
     1801 aaatcgaacg ggcctattcg aagcgagaga ttctcgaact gtacttgaat cagtgctact
     1861 ttggcaaagg cgcttacggc attcaggccg cggcccagac ctttttcagc cggagcgcgc
     1921 cggaattgac cctgccgcag tgcgcgctgc tcatcggcct cctgcaggcg ccgaaccgct
     1981 actcccctct ggagcatccg gagcggacgc tggcgcgccg caacatcgtt ctggatgcca
     2041 tggtggatta caaaaaattg aacgcgaact tcgccgacag tctcaaacgc ctgcctctgg
     2101 agctctcgcc caacgtcgat gtgctgggcg aggcccccta cttcaccgaa atggtccgcc
     2161 agtacttgga agaccgctac ggcgagaaaa tgctcttctc aggcggcctc atgatccaga
     2221 cgaccgtcga cgattccctg cagaaaattg ccgaggaggc cgtgcgctcg gtcatcgatt
     2281 cgctccagaa agatctggag cggagtcaca cgctatccga cccccgctac accgaagaag
     2341 tcgtcgatac ggtgaacggc aagatcgaaa agcggcgcca gtataaacag atccagggtg
     2401 cgtttttggc catcgacaac gccaccggcg acgtgatcac tatggtcggg ggccgggatt
     2461 tcaaccagag caagttcaac cgcgcagtgc aagccctgcg ccagcccggt tcggccttca
     2521 agccgttcgt ttacactgcg gcggtggaca acggctaccg cccctgcgac ctgctcttcg
     2581 attcgcccct ggtgctcacg atccccggct ctggcgagtg gcggccgcag aatatcgatt
     2641 tttccttccg gggccccatg actttgcggg aagggcttca gttctcgcgg aatttaatcg
     2701 ccatcaaact cctgcagaaa attcagcccc agcaggcgat ctactacgcc aagaagatgg
     2761 ggatcacctc gcccttgcag ccggtgccgt cgctcgccat cggcacctcc gaagtctcga
     2821 tgtgggacat ggtgtcggcc tactcggttt tcccgaacgg cggcatccgg atcgaaccga
     2881 agtacatcct gcgcatcacc gaccggtttg gcaacatctt agagaagcac gagacttcca
     2941 agcgggagga agccctctcc cccaccacgg cttacatcat ggtaaatctc ctgcagaccg
     3001 tagtggaccg cggcaccggt gcgcggacgc gggcgatggg gtttacccgg ccggccggcg
     3061 gcaagaccgg gacgaccgac gagttcaccg acaattggtt catcggcttc actccccagt
     3121 tcacggcggc ggcgtgggtg ggattcgacg acaaaacttc catcggctac aaccagaccg
     3181 gctcctccaa cgcgctcccg atctggacca agtttatgct cgctgcgcac gaaaacctgc
     3241 cggtgaaaga tttcacggtg cccccgggta ttgttttcgc caacgtctgc aaagagtccg
     3301 gcgagctggc gaaggatgac tgcccggaga tcctctacaa cgaagttttc accgaagcca
     3361 ccgtccccaa agaacgctgc cacatccact gatcataaga acagcaccgc agaacccgag
     3421 gcgggtggtt gagattattt tttcagaatt cgcttaggtc cccttttctg taaggtcgga
     3481 tactcgtacc ggatcgagga ggatctgggg agggtcagcc cgagaggtag tcgggctcgt
     3541 ccctggccgt atggaaacat ttctcaatcg ggcaggctct gagcccgacc ggttccgacc
     3601 ggctcgggga cgagccggtc tacggtgatt agggatggga acggattgaa agccgcaggg
     3661 ccgaggtaga aattcatacg ggctgtacac accttggcta cggaactata aaaagtgggt
     3721 cgtgagtaag gttctttcgt ggtgcataaa aaaagacaga ggaaccaccg aagtcgcatt
     3781 tttggatgga aggcgacatg ccctctgtct tagacatcga ggaccaatac tttggcactg
     3841 aagtcaaaat aatagttctg gacgggatac tctctctgaa tctggctctc tacaccctcg
     3901 aagcgaatat ttactgagaa gcggagttgc tctctcacat tagttggcgt cgctgagttt
     3961 tccatagcgg cgatggtttg accaaccttt tcgagaagcg tttcaattct gaagtgtcct
     4021 tgaccttggc taagcggttg gaggtaccat ggagattcag ctgagtaaaa tccccccaac
     4081 tcgactggct ggccgtagat agtggcgttg agtttcaccc acatcttgat gttctgttgc
     4141 ttcgtgggat ttccgggaaa ggtcaggatg tgataagggt ctttttcaac ctgtttcatg
     4201 tgaaatgtcc ccaccggaat ccaactggca agggccaaga tgtaggtcaa gtagacgtag
     4261 aaagccactg ccaccagagt gagtgtgagt actacgacct caacagcctt tccgagccgg
     4321 ttgtgctcat accagtacgc ggaagctcca accaacccga gtaccagtat cagcactgcg
     4381 acggggagga atttcgcgaa gaattgtgac tttacgaaag atctaccctg ttttttgagg
     4441 gctttcatat gttcgagaaa atataccata tggattgtat ggggtgggct agatttttcc
     4501 gctgagcata gaaaagatga aattacctga tattttgagc cgactgtgac ccctccctgc
     4561 cctcccctcg aaggggaggg aaagacctgt cgtttaccgc aattctccca ggattttcct
     4621 gagcgggcgc acggcccggc ccgcaaggga aaaacattgt tgcagttgct gcgccacctc
     4681 ccgcccctgt tcttcgtcat ttgcgagcac ggtggccagc gcttcgcccg ccgcgacctt
     4741 ttgccccact tttttgtgaa tcaggaatcc cacggcgtga tcgatcgggt cggtaatctg
     4801 ttttcgcccc gctcctaatt ccacagccag catacctacc cggtaggtat cgatggcttt
     4861 gacccagccg gatttggcac tcgggactga tatgcggtat ctggcctgag gcaaccgcga
     4921 gtagtcgtcc acaatccgtt catctcctcc ctgccttttg accatttccc gaaatttggc
     4981 caaagctgct ccggtct
//
LOCUS       DBNGZD010000187         6271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_99457, whole genome shotgun sequence.
ACCESSION   DBNGZD010000187 DBNGZD010000000
VERSION     DBNGZD010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_99457"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..476
                     /locus_tag="AC16GM_05020"
     CDS             183..476
                     /locus_tag="AC16GM_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR2923076.1"
                     /translation="MATFIGLVSFTDQGVRNVKDTTKRAETVKQMAAKAGGKLKDIYW
                     TLGQYDLVSIWEAPDAETLLPLLVALGAQGTVRTQTLRAFNAEEVGKVISRMP"
     gene            512..1018
                     /locus_tag="AC16GM_05025"
     CDS             512..1018
                     /locus_tag="AC16GM_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019241.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2923077.1"
                     /translation="MSASLATLVIENFRLRLGTDLPKQIRTSLGELTDEQIWWRPNES
                     SNSVGNLVLHLCGSLRHFLGRGVGGSDYQRDRPKEFSEREPIPKAALLRTVDETFAEA
                     QAILGRLTPERLTEMTDRAGAPTPVYELVLRMAIHVAMHTGQILYITKMLKEGVFPEM
                     IWSWSNTR"
     gene            1114..2124
                     /locus_tag="AC16GM_05030"
     CDS             1114..2124
                     /locus_tag="AC16GM_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923078.1"
                     /translation="MTLRWAFVRLGLGAALLLTSLRAAARVPEETAPATAREQEVFDE
                     YIRRMQDNLFRVPLAVTEVLKSYTDHRGRGLELPPIDCRGFLGHKVDRLTVRITTDLR
                     HWQPAYVHRKLSDRTQALYSWQVETEVLGKEKRARANNFIMFDPDAFEQSEREFGPFG
                     VLTNLTAIYHELLHGQLQINAILSDRSWQERACACDFPTEESDADHRFIPAMEKSFLV
                     SLAKVNGFAIYEADGVYAADEDGNFSIIVRDDLLRAKLVNSRQATDSWRWITWDPVNV
                     GRVDVSIEATPRGRYTNDIILRGKLEDPQKPGRFSLYVDPPAVLFLQCYTVEPKRRNA
                     GR"
     gene            2408..2899
                     /locus_tag="AC16GM_05035"
     CDS             2408..2899
                     /locus_tag="AC16GM_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923079.1"
                     /translation="MAYDNNIKEQLDRIEKKLGQATPHPHFFGYEYKSKMKIFGMPLV
                     HIAQGVDPQTGKPRVAKGFIAIGNIAVGVFALGGISLGVFSFAGVGLGIVTFAGCAIG
                     LGIAIGGLAVGTIAAGGLAVGYYALGGAAFGAHTISGSGADPQAVEFFKRFWPGIEKI
                     YNR"
     gene            complement(3244..4608)
                     /locus_tag="AC16GM_05040"
     CDS             complement(3244..4608)
                     /locus_tag="AC16GM_05040"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR2923080.1"
                     /translation="MKRKQAPARKRAAPRPQFAWEGVTPTIIAGGRVIDPASALDRVA
                     DLYLADGRIAKIETKKLLTKRTAPEPAKFIDASGLCVVPGLVDLHTHLREPGREDEET
                     ILTGSEAAVAGGFTSICCMPNTHPPLDTQESIKFIYERAQFVKCRVYPIGAATKQLES
                     KQLTEIGDLVAAGAVAVSDDGRPIADSRLMRLALEYCKMFDIPVINHCEDPALVGDGV
                     MNESFTSTLLGLRGVPSAAEEVMVARDLKLAEYIGGRVHIAHVSTAGAVELIRQAKAK
                     GIQVTAETTPHHFCLTDELIKEKFDTALRVNPPLRTKRDVEAIREGLADGTIDIIVTD
                     HAPHAVEEKEVEFDQAPPGMIGLETALGLVLTELVKPGYLSLSQALAKMTYLPAEILK
                     LKAGKLAPGALADITLFDPDATWTVKAESFRSKSKNSPFIGRQLTGKVRWTLVGGRVV
                     YYVE"
     gene            complement(4598..5533)
                     /locus_tag="AC16GM_05045"
     CDS             complement(4598..5533)
                     /locus_tag="AC16GM_05045"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR2923081.1"
                     /translation="MPLKSRHLLGLEGMTREEIDLVLNTADTFKEVLERPIKKVPPLR
                     GTTVVNLFYEPSTRTRISFELAEKRLSADTVTFTPSGTSVAKGETLRDTTRNLQAMKI
                     DMVVVRHKSPGVPYFLTQCIDASIINAGDGAHEHPTQGLLDLYTIREKYGKIEGLRVV
                     IVGDIQHSRVARSNIWGLKTMGASVAVCGPTTLLPVEISKMGVEVYANLDDTLIGADV
                     VNVLRLQLERQQSGLLPSLREYAIRFGITAERLKRLNPNFTIMHPGPINRGVEITPEV
                     ADGPHSVILQQVTNGVAVRMAVLYLLSGGEIPGET"
     gene            complement(5553..6083)
                     /gene="pyrR"
                     /locus_tag="AC16GM_05050"
     CDS             complement(5553..6083)
                     /gene="pyrR"
                     /locus_tag="AC16GM_05050"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR2923082.1"
                     /translation="MDDKALARALVRIAHEIVEKNHGARGLVIVGIKTRGAHLAERIA
                     EIIAEIEKTKVPTGSLDITFYRDDIQTRLEHPVVQKTELLVPVEGQTVVVVDDVLFTG
                     RTIRAAMDELIDFGRPKQIQLAVLIDRGHRELPIRADFVGKNIPTAPEDNVIVQIKEI
                     DRKDQVLVERKSKGKK"
BASE COUNT         1248 a         1794 c         1908 g         1321 t
ORIGIN      
        1 aaagtctggg cggcaaaacc tgcgtgccgc ccacgccgat tccgaacatg ggcgctttcg
       61 ctatgttcaa cgatcccgaa gggaatatga ttggcttgtt caaagggtag aacaatttcc
      121 agcgagcgag tagctggtag acggtaacgt aaactgttgc tcattattag aggaggtcgg
      181 tcatggcaac tttcatcggt ctggtgagtt tcaccgacca gggagttcgg aacgtcaaag
      241 acaccaccaa gcgggcggaa acggtcaagc agatggcggc taaggccggg ggcaagttaa
      301 aggatatcta ctggaccctg ggccagtacg acctcgtgtc gatctgggaa gcgccggacg
      361 ccgaaacgct tctgccgctt ctggtcgccc tgggtgcaca gggcactgtc cgcactcaaa
      421 ctctgcgggc ttttaatgcc gaggaggtcg gcaaagtaat cagccggatg ccgtaggccg
      481 cgcgattcca agcaaagcat cggatcagtc gatgagtgcc tcactggcga ccctagtgat
      541 agaaaatttc cgcctgcggc tgggaacgga tctccccaag cagattcgta cctctttagg
      601 ggaattgacc gacgagcaaa tctggtggcg gccgaatgag tcgagcaact cggtcggcaa
      661 cttggtcctg cacctctgcg gatcgctgcg gcatttcctg ggccgtggcg tgggcggctc
      721 cgactaccag cgcgaccggc ccaaagaatt ctccgaacgg gagccgatcc cgaaagcggc
      781 gctcttgcgc acggtagatg aaaccttcgc cgaagcacag gcgattctgg gccgcttgac
      841 ccccgagcgc ctgaccgaaa tgaccgaccg ggcgggcgcc cccacgccgg tctacgagtt
      901 agtcctgcgg atggccattc acgtggccat gcacaccggc cagatcctct acatcaccaa
      961 gatgctcaaa gaaggggtct tccccgagat gatctggtcg tggagcaata cccggtaggg
     1021 gaacagcccc ggtgcgcctc gcggcggcaa gacctgtctc gcagggcttt tcccctgtaa
     1081 caattcggct tgattcttcc gatgtaagga aagatgactc tccgttgggc tttcgtgcgc
     1141 ctgggactgg gtgccgccct gctcctgacg agcctcaggg cggcggcacg cgttcctgaa
     1201 gaaaccgctc cagctaccgc ccgggagcaa gaggtgttcg atgagtacat ccgccgcatg
     1261 caggacaatc tcttccgggt gcctttggcg gttacggaag tgctcaaaag ttacaccgac
     1321 caccggggac ggggcctgga gcttccgccg atcgattgcc ggggatttct cggccataag
     1381 gtcgaccgcc tcacggtccg catcaccacc gacctcaggc actggcagcc cgcctacgtt
     1441 caccggaaac tgagcgaccg gacgcaggca ctctactctt ggcaggtgga gacggaggtc
     1501 ctggggaagg aaaagcgcgc gcgggcgaac aatttcatca tgttcgatcc ggatgcgttt
     1561 gaacagtcag agagagagtt tggccccttc ggtgttttga ccaacctcac cgccatctac
     1621 cacgaactgt tacacgggca gttgcagatc aacgccatcc tctccgatcg gtcgtggcag
     1681 gagcgggcct gcgcctgcga ttttcccacc gaagagtccg acgccgacca ccggttcatt
     1741 ccggccatgg agaaatcgtt tctggtaagt ctggccaagg tgaacggctt tgcgatttac
     1801 gaggccgatg gcgtttacgc cgccgatgag gacgggaatt tctccatcat cgtgcgcgac
     1861 gacctcctgc gggcgaaact cgtaaactcc cggcaggcca ccgactcctg gcgctggatt
     1921 acctgggacc cggtgaatgt tgggcgggtc gacgtttcca tcgaggcgac cccgcggggg
     1981 cggtacacga acgatatcat cctgagaggg aaattagagg acccgcagaa gccgggccgg
     2041 ttcagtctgt acgtcgatcc accggcggtg ctcttcctgc agtgctacac agtcgaaccg
     2101 aagcggcgga atgccggtcg gtgaaatgcg cccgtagtgc aggttggata gccctgagcg
     2161 gagtcgaagg gttttcgtaa cctgaaggtg cgggatacgc acctcgactt cgctcggtgc
     2221 aagaaaacca tccctcccac actataagta cactcggaac gggtcggatc gactctcgga
     2281 cgcccaaacc ttttgctcct cttgcaacta aaggtcggta tggcaccgat cccccatgtc
     2341 tcttactact ccgggccgct ggccgccggg catacattag ccgatattaa caagcagagg
     2401 aggtcgcatg gcctacgata acaacatcaa agagcagctc gaccgcattg agaaaaagtt
     2461 gggacaggca acgccgcatc cccatttttt cggctatgaa tacaaatcga aaatgaaaat
     2521 ttttggcatg ccgctggtgc acattgccca gggagtcgat ccccagaccg gcaagccgcg
     2581 cgtggccaag gggtttatcg ccatcggcaa catcgccgtt ggggtctttg cgctcggcgg
     2641 aataagtctg ggcgttttct cgttcgcggg agtggggctt ggtatcgtga ccttcgcggg
     2701 ctgtgccatc gggctgggta tcgccattgg cgggctggct gttgggacga tagctgctgg
     2761 cggcttggcg gtggggtact acgccttggg cggcgcggcc ttcggcgccc acacgatctc
     2821 cgggtctgga gccgatccgc aggcagtcga attcttcaag agattttggc cgggaataga
     2881 gaaaatctac aaccgataga aatactgggt gaatccgcct gaggcggatt cacccctgca
     2941 ggtctcattg ttacagtatg tggcgccggg aggtttttga aaccggcgcc attttttggc
     3001 atagccgagg tcggtacaac ctgcagggga ttctatcctc ggcgcgaccc cacagggttg
     3061 cgtggcatta gaaaaggcga cagcccttcg ggctgccgag gttgaaaacc tcggctacaa
     3121 atcctgttct gacctcaccc tgaccctctc ctaagaggag agggaacttt acgcgagaat
     3181 gattaactcg aattcggaag ggtgccttcc ctcccctgtg ggggagggat agggaggggt
     3241 ctattattcc acgtaataca cgacccggcc acccaccaat gtccagcgca ccttgccggt
     3301 caattgccgc ccgatgaaag gggagttctt cgactttgag cggaagcttt cggcttttac
     3361 cgtccaggtt gcgtcagggt cgaagagggt aatgtcagcc agagcgcccg gagcgagttt
     3421 gccggccttc aatttcagta tttctgcggg caggtaagtc attttcgcca gagcttgcga
     3481 gagggatagg tagccgggtt tgactaattc ggtgaggacc aggcccagcg cggtttccag
     3541 gccgatcatc cccggcggcg cctgatcgaa ctccacttcc ttctcttcca ccgcgtgcgg
     3601 ggcgtgatcg gtgacgatga tgtcaatcgt cccgtcggcc agaccctcac ggatggcctc
     3661 cacgtccctt ttggtgcgca gggggggatt aacccgcaga gccgtatcga atttttcctt
     3721 gatcagttcg tcggtcaggc aaaagtggtg cggggtcgtt tcggcggtga cctggatgcc
     3781 cttggctttg gcctggcgga taagctccac cgcgccggca gtggaaacgt gagcaatgtg
     3841 cacccgtccg ccgatgtact cggccaattt cagatcacgc gcgaccatca cttcttcagc
     3901 cgctgagggc accccgcgca ggccgagcaa tgtcgaagtg aaactttcgt tcatcacacc
     3961 gtcgccgact aaagccgggt cctcgcagtg gttgatcacc gggatgtcga acatcttgca
     4021 gtattcgagg gccagccgca tcaggcggct gtcggcaatc gggcggccgt cgtcggagac
     4081 cgccacggct cctgccgcca ccaaatcgcc gatttcggtc aactgtttac tttccaactg
     4141 cttggttgcg gccccgatag gatagacgcg gcatttgacg aactgcgccc gctcgtagat
     4201 gaatttaatc gactcctggg tatcgagcgg cgggtgcgta tttggcatgc agcagatgga
     4261 ggtgaaaccg cccgcgactg cggcttcgct acccgtcaaa atcgtctctt catcttcgcg
     4321 gcccggttcc cgcaggtgag tgtggagatc aactaatccc ggcaccacac agaggccgga
     4381 ggcgtcaatg aacttggctg gttccggagc ggtacgtttg gtcaggagtt ttttggtttc
     4441 gatcttggcg attcttccgt cagcgagata gaggtcggcc acccgatcca aagccgacgc
     4501 gggatcgatg acccgtccgc cggcgatgat ggtgggcgtt accccttccc aggcaaattg
     4561 tggtcgggga gcggcgcgtt ttcttgcagg cgcttgttta cgtttcaccg ggaatttctc
     4621 cgcccgagag gaggtagagc accgccatgc gcacggcgac gccgttggtc acctgctgga
     4681 ggatgaccga atgcgggccg tcggccactt cgggcgtgat ttcgacgccg cggttgatgg
     4741 gccccgggtg catgatggtg aaatttggat tcagtctttt gagccgttcc gccgtaatgc
     4801 cgaagcggat ggcatactcg cgcagggaag ggagtagtcc cgactgctgg cgttccaatt
     4861 gcaggcgcag gacgttgacc acgtcggcgc cgatcagggt gtcatcgagg ttagcgtaga
     4921 cctcgacccc catttttgag atttccaccg gcagaagcgt ggtggggccg cagaccgcca
     4981 ctgaagcacc catcgttttc aaaccccaga tgttcgagcg ggccacgcgg ctgtgctgga
     5041 tgtcgcccac gatcaccacc cgcaggcctt cgattttccc gtacttctcg cggatggtgt
     5101 acaagtccag aagtccctgg gtcgggtgct cgtgtgcgcc gtcgccggcg ttgatgatcg
     5161 aggcgtcgat acactgcgtc aaaaagtacg gcacccccgg cgatttgtgc cggaccacca
     5221 ccatatcgat tttcatggcc tgaagattgc gggtagtatc gcgcagggtc tcgcctttgg
     5281 cgactgaagt gcccgacgga gtaaaggtga cggtgtcggc ggagaggcgc ttttcggcta
     5341 actcgaacga gatgcgggtg cgggtggaag gctcgtagaa aagattgacc actgtagtcc
     5401 cccggagagg cggaactttt ttgatcggcc gctccaagac ctctttgaag gtgtcggcgg
     5461 tgttcagaac gaggtcgatt tcctcgcggg tcattccttc cagacccaga aggtggcggc
     5521 ttttcagcgg cataggcagc gacgatccga tttcatttct tgcctttgct tttgcgttcg
     5581 accagcacct gatctttgcg gtcgatttct tttatctgta cgatcacgtt gtcctccggc
     5641 gccgtgggaa tgttcttccc cacgaagtcg gcgcgaatgg ggagttcgcg gtggccgcgg
     5701 tcgatcaaga ccgctaattg aatctgtttg ggccggccaa agtcgatcaa ctcgtccatc
     5761 gccgcgcgga tggtgcggcc ggtgaagaga acgtcgtcca ccacgacgac cgtctgcccc
     5821 tccacaggta ccaacaattc cgtcttctgg accaccggat gttcgaggcg tgtctggatg
     5881 tcgtcgcggt agaaggtgat gtcgagcgac ccggtgggga ctttggtctt ctcgatttcg
     5941 gcaataattt cggcgatgcg ttcagccaaa tgagcgccgc gggtcttgat accgacgatg
     6001 accagtccgc gcgcgccgtg gtttttctcc acgatttcat gggcaatgcg caccagagca
     6061 cgggccagcg ccttgtcgtc catcacctgc gtacgctggg cggtcacgag cacctcaccg
     6121 gcataaaaaa tccctccgct cgacagaggg aggggcgaat ttttccacga cacataggtt
     6181 caacaggctt tcctttcccg atctcaccgg atcgggttaa aaggtcaccc tctaagttaa
     6241 cacgggaaaa gaaaaagtca aggccaagaa g
//
LOCUS       DBNGZD010000188         6542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_446382, whole genome shotgun sequence.
ACCESSION   DBNGZD010000188 DBNGZD010000000
VERSION     DBNGZD010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_446382"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="AC16GM_05055"
     CDS             <1..1174
                     /locus_tag="AC16GM_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR2923083.1"
                     /translation="KHYAVHEMKIALFPTQPGRQTVGSASIQCTVEDLERFFSRDPFD
                     QFGADLRSFFNQGQTKILRTDPITLEVRPLPEAGKPGSFSGAVGNFTLQGACDRTSVP
                     VGQPVTLKMTISGTGNLKAIAEPKLPELSDFRTYASGSSEKIEHAGNRIAGTKTFEEV
                     LIPKAPGKFQVPAITLSFFDPARGKYQTVKAGPFVITATGSAEQIASLPASGEGTPVV
                     ASDLRYLKTNADHLSGSAQPVIFRLWFWGLQLVPLLGLGGLVAYQKHQWKLATDVGYA
                     RSRRAHRMAKKRLATALDLIGINGQKEFFAEVSKAVLLYVADKFNTSTYGMTTPEMED
                     FLKTKGVSPELVKRLTELLTSCDLAKFAPTYVTQTDMKKKLDEAEEMIVSLEGVRR"
     gene            1171..1917
                     /locus_tag="AC16GM_05060"
     CDS             1171..1917
                     /locus_tag="AC16GM_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR2923084.1"
                     /translation="MKFRFALSAGALAALLLLPSPGEAFPLEKFHAANALYDQGNFSG
                     AIAGYEGLLSSGTQDYRAYYNFANAYYRDKKIGYAFLNLLRAQRLAPRDDDIRTNLAF
                     LKQQALDKFPQLPDPVGSLWRPLALNELAWLFSLFYFLTIGSFAAWRFRVYPVRVFRW
                     GAVTFGLVALIFLGLLYGKYQSDYAQDHGVVIAQQVEVKSGPGEDFLTQFTGHEGLTL
                     TVLQMRPDWYEALFPNGVKGWVKKETVELL"
     gene            2009..2296
                     /locus_tag="AC16GM_05065"
     CDS             2009..2296
                     /locus_tag="AC16GM_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR2923085.1"
                     /translation="MVTTVTLKELRPKLPEVIERVDGRMDRYIVTRRGKPVAVMMNVD
                     EYESWMETLNLMGDKKTLVRLRRAEQEIGRGNVRPLEEVLQRIETRQTKKR"
     gene            2293..2568
                     /locus_tag="AC16GM_05070"
     CDS             2293..2568
                     /locus_tag="AC16GM_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR2923086.1"
                     /translation="MTGAPPYRVEVPHQVEKTLRRLRASTPRIYERVTAILQDLATDP
                     FSGKGLKGAFIQYRSYRVGDYRILYRIETKAKVIYIVDIGHRREIYR"
     gene            complement(2629..5514)
                     /gene="uvrA"
                     /locus_tag="AC16GM_05075"
     CDS             complement(2629..5514)
                     /gene="uvrA"
                     /locus_tag="AC16GM_05075"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR2923087.1"
                     /translation="MSQSVIRIKGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDFIDGLSPAISIEQRSAAKNPRSTVGT
                     VTETYDYLRLLYARIGVPHCYRCGRKISQQATDQIVDAVLDLPRSTKIQILAPLVSGR
                     KGEHKEVFDEAKRKGFVRVRVDGKVLELGPEIKLDKKKKHSIEVVVDRLIVEPKIKKR
                     LADSLETALSTGGGLVMVERVGSSTATPIKSRSAKGAKPTELLQFSQHFACPHCGISY
                     EEPAPRLFSFNSPFGACPTCDGLGTKMEIDPDRIIPDPNLSINQGAIAPWGDAIGSWY
                     RQMLTSVADHYRFSLHTSWSRFSEEARHIVLYGTGKKELRFTYEHREGKGHGEYTGQF
                     EGVIPNLERRYRKTESAGVREWIEKFMAVRPCPTCNGARLKKEALAVKLNGLSISELT
                     AFTVKKTREFFKDLTLTKREQVIAKQILKEINERLGFLVNVGLDYLTLDRSATSLSGG
                     EAQRIHLATQIGSRLMGVLYILDEPSIGLHQRDNKRLLNTLIGLRDLGNTVVVVEHDR
                     ETIQTADFVVDLGPGAGKNGGEVVVSGPPEVVARTPRSITGQYLSGKRFIPIPQRRRN
                     GSGKYVKLIGATGNNLKNVTVQIPLGLFVCVTGVSGSGKSTLINETLYRILAQDFFLA
                     KTPPLSYQKIEGLQKIDKVIDIDQSPIGRTPRSNPATYTGVFTFIRDLFAAHPEAKMK
                     GYAPGRFSFNVRGGRCEACEGDGIIKIEMHFLPDVYVPCEVCKGKRYNRETLEVKYKG
                     KSIADVLDMTVDETLQFFENIPRIRKKLKTLADVGLGYIHLGQQATTLSGGEAQRVKL
                     STELSKTATGNTLYILDEPTTGLHFEDIKMLLNVLNELVDRGNTVLVIEHNLEVIKTA
                     DYIIDLGPEGGDDGGRIIAQGTPEQVAQAKESYTGQFLREILGNGKK"
     gene            complement(5734..>6542)
                     /locus_tag="AC16GM_05080"
     CDS             complement(5734..>6542)
                     /locus_tag="AC16GM_05080"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR2923088.1"
                     /translation="PWNPLFTTKNIPRIVGGVTPACTWVARTFYQAIVQNVHTVSSPR
                     AAEMVKLLENTFRSVNIGLVNEMALMCDRLKIDVWEIIEAATTKPFGFMPFYPGPGLG
                     GHCIPIDPHYLSWKLKTLNYYARFIELAGDINSHMPEYVVERIIRALNDRKRSLNGAR
                     ILVLGVTYKRDIQDIRESPALDVIRLLQDRGAQVTYNDPHVPKVELDSIEMRSTKLSA
                     GLIRTVDCVTIVTDHSAYDYDWVVANARLVLDTRNATKDVTQGREKIVKL"
BASE COUNT         1332 a         1858 c         1886 g         1466 t
ORIGIN      
        1 caagcactac gcggtgcacg agatgaaaat cgcccttttt cccacccagc cgggcaggca
       61 gaccgtcggc tccgcctcaa ttcagtgcac cgtcgaagac ctggagcggt tcttttcccg
      121 cgatcccttc gatcagttcg gcgccgacct gcgctcgttt ttcaaccagg ggcagacgaa
      181 aattctccgc accgacccca ttaccctcga ggtgcggccc ttgccggaag cgggcaagcc
      241 ggggagtttc tcgggagccg tgggaaattt taccttgcag ggggcctgcg acagaacctc
      301 cgttccggtg ggccagcccg taacgctcaa gatgacgatc tcgggcacgg gcaacctcaa
      361 ggccattgcc gagccgaagt tgcccgaact ctccgacttc cgcacgtacg cttcggggtc
      421 gtcggagaaa atcgagcacg cgggcaatcg catagccggt accaaaacgt ttgaggaagt
      481 tctcattccc aaagctcccg gcaagttcca ggttccggcg ataaccttat ccttttttga
      541 tccggcgcgc ggcaaatacc aaacggtaaa ggccgggccg ttcgtcatca ccgctaccgg
      601 tagtgccgag caaattgcct cactgcccgc ttccggggag ggcacgccgg tggtggcgtc
      661 cgacctccgc tacctcaaaa cgaatgccga ccacctttcc ggttcggccc agccggtgat
      721 cttccggctc tggttctggg gcctgcaact ggtaccgctt ctgggtctgg gcgggctggt
      781 ggcctatcag aaacaccagt ggaaactggc gaccgacgtg ggctacgccc gctcccgccg
      841 tgcgcaccgg atggccaaaa aacgcttggc caccgcgctt gatctgatcg gcatcaacgg
      901 ccagaaagaa ttttttgccg aagtgtcgaa ggcagtattg ctctacgtcg cagataaatt
      961 caatacctcc acgtacggga tgaccacgcc ggaaatggaa gatttcctca aaaccaaagg
     1021 ggtatcaccc gaactggtga aaagactcac cgaacttttg accagttgcg acctggccaa
     1081 atttgcgccg acctacgtga cccagaccga tatgaaaaag aagttagacg aggccgaaga
     1141 gatgatcgtg tcgctggagg gcgtgcgccg gtgaaattcc gttttgccct ctcggccggc
     1201 gcgcttgcgg ccctgctttt gctcccctca ccgggagaag cgtttccact ggagaaattc
     1261 cacgccgcca atgcgctcta cgaccagggg aatttcagcg gtgccatcgc cggatacgag
     1321 ggtctgcttt cttcgggaac gcaggattac cgggcctact acaactttgc caatgcctac
     1381 taccgggaca agaaaatcgg ctatgccttt ctgaacctcc tgcgcgccca gcgcctggcc
     1441 ccgcgggatg atgacatccg gaccaatctt gcttttttga aacagcaggc cttggacaaa
     1501 ttcccccaac tgcccgatcc cgttggctcg ctctggcggc ccttggccct gaatgaactg
     1561 gcctggcttt tttcgctctt ttacttcctg acgataggtt cttttgccgc gtggcggttt
     1621 cgggtctatc cggtgcgggt gttccgctgg ggtgcggtca cgtttggact ggttgcttta
     1681 atttttctcg gtctgctcta cgggaaatac cagagcgatt acgcgcagga ccacggtgtc
     1741 gtgattgccc agcaggtgga ggtgaaaagc ggtccgggcg aggacttctt aacccagttc
     1801 accggccatg agggattgac cctcacggtc ctacaaatgc gccccgactg gtacgaagcg
     1861 ctcttcccca acggggtaaa agggtgggtg aagaaagaga cggtggagtt gctctaagtc
     1921 cgcactgaca agcatcggtc tccagacgaa gtcgccgaac tgaaaagggt actattaaag
     1981 tttacacaat tgcgtatact tacttactat ggtgacaacc gttactctga aggaactccg
     2041 ccccaaattg cctgaagtca tcgaacgggt cgacggccgg atggaccgct acatcgtcac
     2101 ccggcgcggg aagccggtcg ccgtgatgat gaatgtggac gagtacgagt cctggatgga
     2161 gacgctcaac cttatggggg ataagaaaac tttagtccgc ctgaggcggg ccgaacagga
     2221 aatcggacgg ggcaacgtac gtccgctcga agaagtttta cagcgcattg aaacacggca
     2281 gactaagaag cggtgaccgg cgcgccgcca taccgcgttg aggttccgca tcaggttgag
     2341 aaaaccctgc gccgcctgcg agcttccacg ccacgaattt acgaaagggt tacagcgatt
     2401 cttcaagatc tggccactga cccgttttca ggtaaagggc ttaagggggc gtttatccag
     2461 tatcgttctt accgggttgg agattaccgt attctctacc gcatcgagac gaaggccaaa
     2521 gttatctaca tcgtggacat cggccaccgg cgagagatat accggtaaat agttttcttt
     2581 cgatctgatt cttctgctcc atctgctatg aattcatcct gcggttcatc atttctttcc
     2641 attccccagt atctccctta aaaactgccc ggtgtacgac tctttggcct gcgccacctg
     2701 ctccggcgtg ccctgagcga tgattctccc cccgtcgtcg cccccttccg ggcctaagtc
     2761 gatgatgtag tcggcggtct taatcacctc caggttgtgc tcgatcacca ggaccgtatt
     2821 gccccggtca actaattcgt tcaggacgtt cagaagcatc ttgatgtcct cgaagtgcag
     2881 gccggtcgtg ggctcgtcca aaatgtagag cgtattgccg gtggcggttt tggagagttc
     2941 ggtcgaaagt ttcacccgct gggcctcgcc gccggaaagg gtcgtggcct gctggcccaa
     3001 gtggatatag cccagaccca cgtcggccag cgtcttcagt tttttgcgga tgcgcgggat
     3061 attctcaaag aattgcaggg tttcgtccac agtcatatcg agcacgtcgg caatcgattt
     3121 ccctttatac ttcacctcca aagtctcgcg gttgtagcgc ttccccttac agacttcgca
     3181 gggaacgtaa acgtcaggca aaaagtgcat ctcaattttg atgatgccgt cgccctcgca
     3241 agcctcacac cggccgccgc ggacattgaa agaaaaccgc cccggggcgt agcccttcat
     3301 cttcgcctct ggatgtgctg caaaaaggtc gcggatgaaa gtgaaaacac cggtgtaggt
     3361 ggctggattg gagcggggcg tccggccaat gggcgactgg tcgatgtcga tcaccttgtc
     3421 aattttctgc aacccctcaa ttttttgata agagagtggc ggcgttttcg ctagaaaaaa
     3481 atcctgagcc aggattcggt acaacgtctc attgatcaaa gtcgacttcc ccgagccgga
     3541 aacgccggtg acgcagacga aaagtcccag cggaatttgg acggttacgt tcttcaaatt
     3601 gttgccggtg gcgccgatta acttcacata cttgccgctg ccgttgcgcc ttcgctgggg
     3661 aattggaatg aaacgcttgc cggataaata ctggccggtg atggagcgcg gggtacgggc
     3721 gaccacctcc ggcgggccag agacaaccac ttcgccgccg ttcttgcccg ctccgggacc
     3781 taaatcaacc acaaaatcgg cagtctgaat cgtctcccgg tcgtgctcca cgacgacgac
     3841 ggtgttgcct aaatcgcgca ggccgatgag cgtgttcaaa aggcgtttat tatcccgctg
     3901 gtgcaggccg atggagggtt catccagaat gtaaaggacg cccatcaagc gggagccgat
     3961 ctgggtggcc aggtgaattc tttgggcttc gccgccggag agcgaggtag ccgagcggtc
     4021 caaggtgagg tagtcgaggc ccacattgac cagaaacccc agccgctcgt tgatttcttt
     4081 caagatctgt ttggcaatca cctgttcgcg cttagtcaaa gtcaaatctt tgaagaactc
     4141 tcgggttttc ttgaccgtga aggcggtcaa ttctgatatg gaaagtccat tcagtttcac
     4201 cgccagggct tcttttttca agcgcgcgcc gttgcaggtg gggcagggcc gaaccgccat
     4261 gaacttctca atccactccc gcacccctgc cgattcggtt ttccggtagc gccgctccaa
     4321 attggggatg accccttcga actggccggt gtactcgcca tgccccttcc cttcccggtg
     4381 ttcgtaggtg aagcgcagtt cctttttgcc ggtgccgtac aggacgatat gccgggcctc
     4441 ttcgctgaag cggctccagg atgtgtgcag ggagaagcgg tagtggtcgg ccacgctcgt
     4501 aagcatttgc cggtaccagc tgccgatggc gtccccccag ggtgcgatgg caccctggtt
     4561 gatggaaagg ttcgggtcgg gaatgatgcg atcgggatcg atctccatct tggtgcccag
     4621 gccgtcgcag gtggggcagg caccgaaagg cgagttgaac gaaaagaggc gcggcgccgg
     4681 ctcctcgtac gagatgccgc agtgggggca ggcgaagtgc tgggagaact gcaaaagctc
     4741 ggtcggcttg gcacctttcg cgctccgcga tttgatcggg gttgcagtgg acgaaccgac
     4801 gcgttccacc atcaccagcc cgccgccggt ggagagtgcg gtctccagcg aatcggccag
     4861 gcgctttttg attttgggct cgacgataag gcgatctact accacttcga tactgtgctt
     4921 ctttttcttg tcgagtttga tttccggccc caactccagc actttgccgt ccacccgcac
     4981 ccggacgaag cccttccgct ttgcctcgtc gaagacctct ttatgctcgc ccttccgccc
     5041 actcaccaga ggcgctaaga tttgaatttt cgtgctccgg ggcagatcga gcaccgcatc
     5101 gacgatctgg tcggtcgcct gctggctgat tttcctcccg caccggtagc agtggggcac
     5161 gccgatccgg gcgtacaaaa gtctcaggta gtcgtaggtt tccgttacgg ttcccacggt
     5221 ggagcggggg tttttggcgg cggagcgctg ctcgatggag atggcgggcg aaagcccgtc
     5281 aatgaaatcg acgtccggtt tctccatcag gcccagaaac tgccgggcgt aggccgaaag
     5341 cgactccacg tagcggcgct ggccttcagc gtaaatggtg tcgaaggcca aagacgactt
     5401 ccccgatccg gacaggccgg tgatcaccac cagcttatcg cggggaatct ctacatcgat
     5461 attttttagg ttatgctcgc gggcgccttt gatgcgaata accgactgcg acaaagcgaa
     5521 acctccattt tgggcaaccg gtgaatatac aacccaaggg gtggaggttc aatcagattt
     5581 ttcagaaaaa gttcttaacc tttgtcctac gaccttcgct gagtgctatc tgcctttttc
     5641 caaggagggg aattagggaa acactgcggg tgcacaaaga gtatcggttt tctccgtgca
     5701 ctctgtgctt ctgtggtgaa agtcttggct tttctacaac ttcactatct tttcccgtcc
     5761 ctgagtaacg tcttttgtcg cgttacgggt gtccagcacc aggcgggcat tggccaccac
     5821 ccagtcgtag tcgtaggcgc tgtgatcggt gacgatcgta acgcagtcga cggtccggat
     5881 caggccggcc gagagtttcg tcgagcgcat ctcgatggag tccagttcaa ctttgggcac
     5941 gtgcgggtcg ttgtaggtca cctgcgcgcc gcggtcttgc aagaggcgaa tgacgtccaa
     6001 agccggtgat tcccgaatgt cctggatgtc ccgcttgtag gtcacgccca gcaccagaat
     6061 cctggcaccg ttgagacttc ttttccggtc gttgagcgcc cggatgattc gctccaccac
     6121 atattccggc atatgcgagt tgatgtcgcc ggccagttcg atgaaacggg cgtagtagtt
     6181 cagggtcttc agtttccatg agaggtagtg ggggtcgata ggaatgcagt ggccgcccaa
     6241 ccccggtccg gggtagaacg gcatgaagcc gaagggcttg gtggttgccg cttcgatgat
     6301 ttcccagacg tcgattttga gccggtcgca cattaaagcc atctcgttca ccaggccgat
     6361 attgaccgag cggaaggtat tctccaggag tttcaccatt tccgcggccc ggggagagga
     6421 aacggtgtgg acgttttgga cgatagcctg gtagaacgtg cgggccaccc atgtgcaggc
     6481 aggggtgacg ccgccgacaa tccgggggat gttcttggtc gtgaagaggg gattccaggg
     6541 gt
//
LOCUS       DBNGZD010000189         2908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1349807, whole genome shotgun sequence.
ACCESSION   DBNGZD010000189 DBNGZD010000000
VERSION     DBNGZD010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1349807"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /locus_tag="AC16GM_05085"
     CDS             <1..1811
                     /locus_tag="AC16GM_05085"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR2923089.1"
                     /translation="RRRTERVGVSAPAEPASGGPAPGVRAETKPKLPPVKPISEFEFE
                     PTSPEEFEKLLPLYESTFKTVREGELVKGRVLRITRDDVFLDVGFKSEGIIPLVEFGE
                     NPEVKPGDEIDVFLESFEDQNGQLVLSKQKADFMRVWDNIKEASDRNQPITGRAVRRI
                     KGGLVVDLMGVEAFLPGSQIALRQQFDVDTLLGQALEFKIIKLNKARRNIVVSRRAVL
                     EEQREVMKSKLLAEMAVGQTREGYIKNITDFGVFVDLGGVDGLLHITDMAWGRVGHPS
                     EMVNVGQKINVKILDFDKNTGRISLGLKQLTPYPWENVEQKFPVGSRVRGRVTSITDY
                     GAFVELEKGLEGLVHISEMSWTHHVKHPSKILSANDVIEAVVLNVDREHEKISLGLKQ
                     LTADPWDTLELRYPVGIKVTGKVRNLTPFGAFVEVEEGIDGLIHVSDMSWTRRIQHPS
                     EVLKKNDVVEVKVLHIDKENRRISLGLKQVQEDPWPKLAEKYRQGTEVEGKVMRLLER
                     GVVVDIQNELEGFVPLNHLGVSDLAKPQDAFKEGETIPLRVLEFDQSGRRLMLSVDAY
                     FQNRDKKELEEYRSKHPVKKAKEEPVGAAQEKEEQG"
     gene            1920..2423
                     /locus_tag="AC16GM_05090"
     CDS             1920..2423
                     /locus_tag="AC16GM_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2923090.1"
                     /translation="MAGNVKPIPEGYHSLTPHLVVNDGRRAVEFYQKAFGAEVRGVFQ
                     DSPDGKVHHAELKIGDSLFMLADEFPMEPKAGMKFLSPKSLGGSTTTLHLYVENVDAA
                     FDRAVAAGAAVAMPLMDAFWGDRYGQLLDPFGHRWALATRKENLSQEEVKKRGEAFFA
                     QWAKQKK"
     gene            2468..>2908
                     /locus_tag="AC16GM_05095"
     CDS             2468..>2908
                     /locus_tag="AC16GM_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923091.1"
                     /translation="MQSALAKEKIGDAALKAKTGKTWREWYAILDKAGARKMNHTEIA
                     AHLYEKEKVPGWWCQMVAVGYEQARGLRQKHERPDGFEISASKTIAASLATIYRAWKD
                     EKLRQRWLPKVTFSLTTDTPNKCLRMKWGDGSERVDVAFFPKGTG"
BASE COUNT          692 a          804 c          896 g          516 t
ORIGIN      
        1 cgcgcagaag aactgagaga gtcggcgtaa gcgcgccggc agagcccgcc tcaggcggac
       61 cggcaccggg ggttcgcgcc gagaccaaac cgaaactgcc gccggtcaag ccgatttccg
      121 agtttgaatt cgaacccacc tcgccggaag aattcgaaaa acttttgcct ctgtacgagt
      181 cgaccttcaa gaccgtccgc gaaggggaac tggtcaaagg ccgggtcttg cgcattaccc
      241 gcgacgacgt cttcctcgac gtcggcttca agtcggaagg gattatcccc ttagtcgaat
      301 tcggggagaa tcctgaggtc aagccgggcg atgaaattga tgttttctta gaatcgttcg
      361 aagaccagaa cggccagtta gttctctcca agcagaaggc cgacttcatg cgcgtctggg
      421 acaacatcaa ggaggcctcc gaccggaatc agcccatcac gggccgggcg gtccggcgca
      481 tcaaaggcgg cttggtcgtt gacctgatgg gcgtggaggc gtttctgccc ggttcgcaaa
      541 tcgctttgcg gcagcagttc gacgtggata ccctcctggg ccaggcgctg gaattcaaaa
      601 tcatcaaact gaacaaagcc cggcgcaaca tcgtggtctc ccgccgcgcg gttttagaag
      661 aacagcgcga ggtgatgaaa tcgaaactcc tggcggaaat ggcggtgggc cagacgcggg
      721 aggggtacat taaaaacatc accgacttcg gcgttttcgt cgatttaggc ggcgtcgacg
      781 gcctcctgca catcaccgac atggcctggg gccgggtggg tcaccccagc gagatggtga
      841 atgtcggcca gaaaatcaac gtcaaaattt tagatttcga taaaaacacc gggcgcattt
      901 cgctgggctt gaagcagctc actccctacc cctgggagaa cgtggagcaa aagttcccgg
      961 tgggctcccg cgtgcgcggc cgggtgacct ccatcaccga ctacggtgcc ttcgtcgagc
     1021 tggagaaggg gctggagggc ctggtgcaca tctccgagat gtcctggacc caccacgtca
     1081 agcacccgtc gaagattctc tccgcgaacg acgtgatcga agcggtggtg ctgaacgtgg
     1141 accgcgagca cgaaaaaatc tctctgggct tgaagcagct gaccgccgac ccctgggaca
     1201 ccttggaact ccgctacccg gtggggatca aagtaaccgg caaggtgcgc aacctgacgc
     1261 ctttcggcgc tttcgtggaa gtcgaggaag ggatcgacgg cttgatccac gtctccgata
     1321 tgtcctggac gcggcggatt cagcacccct ccgaagtgct caagaaaaac gacgtggtgg
     1381 aagtgaaggt gctccacatc gacaaggaga accgccgcat ctcgctcggt ttgaaacagg
     1441 tgcaggaaga cccctggccg aaattggccg aaaagtaccg gcagggaacc gaagtcgaag
     1501 gcaaagttat gcgccttttg gagcggggcg tggtggtgga catccagaac gaactcgaag
     1561 gttttgtgcc gctcaaccac ctgggtgttt ccgacctcgc caagccgcag gatgcgttca
     1621 aagagggcga gacgattccc ctgcgcgttt tggaattcga ccagtcgggc cggcgcctga
     1681 tgctctcggt cgatgcctac ttccagaacc gcgacaaaaa ggaattggaa gagtaccgtt
     1741 ctaaacatcc ggtgaaaaag gccaaggagg agccggtcgg agcagcgcag gagaaagagg
     1801 agcaagggta aggaagagca atacattcac agcagataaa gcagattaaa cggaaagaga
     1861 gtaacaacag gcactccccg gggtgagctt tctttggttt ttttaaggag gatcctccaa
     1921 tggccggaaa cgtaaagccc attcctgaag gttaccactc gctgaccccg cacttagtgg
     1981 taaatgacgg ccgccgggcc gtcgagtttt accagaaagc cttcggcgcc gaggtgcgcg
     2041 gcgtttttca ggacagtccc gacggcaaag tgcaccatgc cgaactcaaa atcggcgatt
     2101 cgctctttat gctcgccgac gagttcccga tggaaccaaa agcgggaatg aaattccttt
     2161 cgccgaagtc tttaggcggg agcactacga ccctgcatct ctacgtggaa aacgtggatg
     2221 ccgcctttga ccgggccgtt gccgccggcg ctgctgtggc catgcccctg atggacgcct
     2281 tttggggcga ccgctacggg cagttgcttg atcctttcgg ccaccgctgg gctctggcga
     2341 cgcgcaagga gaacctatcg caagaagagg tcaaaaagcg cggcgaggcc ttctttgcgc
     2401 agtgggcgaa gcaaaaaaag taaccggact ctcgaggggc acaatcatcg ggggaaagtg
     2461 aggaacgatg cagagcgcgc tggccaaaga aaaaattggt gacgcggcct taaaagcgaa
     2521 aacaggcaag acctggcgcg agtggtatgc aattctcgac aaagccggcg ccagaaagat
     2581 gaatcacacc gagattgccg cccacctcta cgagaaagag aaagtccccg gctggtggtg
     2641 ccagatggtg gcggtgggct acgagcaggc gcgcggcctg cgccaaaagc acgagcgacc
     2701 cgatggtttt gaaatcagcg cgagcaagac catcgccgcg tcgcttgcga cgatctaccg
     2761 ggcctggaag gacgaaaaac tgcgccagcg ctggctgccg aaagtaacct tctcgcttac
     2821 gaccgataca cccaacaagt gcctgcggat gaaatggggg gacggctccg agcgcgtgga
     2881 cgtggcgttc ttcccgaaag gcacgggg
//
LOCUS       DBNGZD010000190         6721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_794408, whole genome shotgun sequence.
ACCESSION   DBNGZD010000190 DBNGZD010000000
VERSION     DBNGZD010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_794408"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1000
                     /gene="pheS"
                     /locus_tag="AC16GM_05100"
     CDS             2..1000
                     /gene="pheS"
                     /locus_tag="AC16GM_05100"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR2923092.1"
                     /translation="MESIRAEAERAIAAAPDEKDLDQVRVTYLGRKGRLTAILRNLKE
                     VPEGRRPQVGQAANAVKTFLEGLIAQKETELKTSARPAAPAFDYTLPGRGHAVGKLHL
                     VTQTTDAIVRIFSRMGFDTAYGPEVELDYYNFAALNFPPEHPARDMQDTFYVSEDVVL
                     RTHTSPVQVRAMEKRQPPLKILSPGRVYRHESINVRSHVLFHQVEGFAVDKDITFGDM
                     EGVLSVLAWELFGRDVGIRFRPSFFPFTEPSAEVDISCTICGGKGCGVCKNEGWVEIA
                     GCGMIDPEVFRSVGYDSELYTGYAFGLGVERVAQLKHRIDDVRLFLENDLRFLRQF"
     gene            1102..3486
                     /gene="pheT"
                     /locus_tag="AC16GM_05105"
     CDS             1102..3486
                     /gene="pheT"
                     /locus_tag="AC16GM_05105"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR2923093.1"
                     /translation="MKVSYNWLRELIDFPWDPKELAEKLTLAGLEVGSVTPQYPNLDG
                     VVVGRIESVERHPQDEHLFYCQVNIGRERLAIVSGAPNTVSGVKVPVAPPDTVLPDGT
                     LVQKKTIRGVESFGMVCSERELGLSDDHSGLMILDGDSEIGQPLARTLNLTDVILDID
                     LTPNRGDALSHIGVARDIAGLTGNRIRRPKIEFKEIEKPAAAAVEITLDDPVGCPRYS
                     ARVLARIKVGRSPWWLRQRVEACGMRAINNVVDVTNYVMLERGHPLHAFDYDLFSRKK
                     VVVRRAKPKEKFTTLDGFERELNEKVLLITDDKIPVAIAGIMGGEKSEVAEKTTNILL
                     ESAYFDPVVIRVGRKSLGLQTDASNRFERGTDPNGLLYAVNRAAGLLARLAGAEVCAG
                     VVDVYPKIIEPVMLTLRPSRVNKILGTEIPSPEMVTILTNLELGVTAGKELQVTVPTF
                     RPDVTREIDLIEEVARIWGYEKIPRSHSARGDAYVPTHAEDELTSAIKNFLCAQGYNE
                     VVTNTLVDPGWLEKLGLPASPVRLQNPIAEALSVLRTGLVSSLLEVVRRNRNRQADLI
                     KLFEVGRIFLSSREKLPREKLVLGIVATGIRQRQSWDAAEAPVDLFDLKGVLDSLAQR
                     FRLNGFALEPKAVPFLDPESSFRLLLNGAEVGSLGAVGSEAQEFFDLSDAVYYAECDF
                     GALLAAGVSRPTYEPLDRYPFVERDIAVVVDRTCLAGDLLAAIRQTGGELVREARLFD
                     LYQGQPLPLDKRNLAFRLRYQSRERTLTDREVDAIQERIVAALKKQFDAALRTY"
     gene            3531..3827
                     /locus_tag="AC16GM_05110"
     CDS             3531..3827
                     /locus_tag="AC16GM_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923094.1"
                     /translation="MAQDVLQKLQDRVNQTLETVEKLKQENQTLSAGRHALTEQLALA
                     QKNLTALTVQYQKMQKEKERQDQDYQKRKVALRQKLETVLTKVATVEERLRNFT"
     gene            3924..4241
                     /locus_tag="AC16GM_05115"
     CDS             3924..4241
                     /locus_tag="AC16GM_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR2923095.1"
                     /translation="MTSASTPTDQTTLRVNIFGEEYPVRAKADPDYITAVAKYVDQKM
                     RDIAQKIPNRSFANVAVLAALNITDELFRERQDKEKKLTDVEEKTQEILVWLDQKLAE
                     GQI"
     gene            4339..5904
                     /gene="rny"
                     /locus_tag="AC16GM_05120"
     CDS             4339..5904
                     /gene="rny"
                     /locus_tag="AC16GM_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR2923096.1"
                     /translation="MTNFILILIIAAAAVAAGIALGFWLSRRIGAEKLARAGELAQRM
                     IREAEKEAEIKKKEALLEAKDEWYRAKQNFERELQNKKLEFQKTEKRFQEREAEVARK
                     VDFITKKERELQNREKVLLAREKGITTREGELERLVVEQNRQLERVAQMTTEEAKKQL
                     MENLINQARAEAAQTIKQMKDEAEQTGEREARNIITLAIQRCAADHTVETTVSVVNLP
                     SDEMKGRIIGREGRNIRAFEAATGCDVIVDDTPEAVVLSGFDPVRREIARMALEKLIL
                     DGRIHPARIEEVVTKTQKEMETIIREAGEQACFELGIHNLHSEVVKTLGRLKFRTSYG
                     QNVLAHSQEVAMLCGLIAAQLGLDPQIAKRAGLLHDIDKAIDRETEGTHTKIGLNFMS
                     KYNEDPAVLNAIAAHHEDVIQETPYAVLVQAADAISGSRPGARRESLEGYVRRLEKLE
                     EVADSFKGVAKAYAIQAGREVRVMVENDQVDDSGAAELAGQIAKKIQAEMEYPGQIKV
                     TVIREMRAVDYAR"
     gene            5984..6622
                     /locus_tag="AC16GM_05125"
     CDS             5984..6622
                     /locus_tag="AC16GM_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HIR2923097.1"
                     /translation="MKISFTGFWFLGLFGTLVVLERVNLPKRESSFGQRLLTAAREYL
                     QEKPTYDLSRRYRKIGFPKGDIRRNKGVCADLVIRVYREVGIDLQERMHEDMKKNFHI
                     YLARRNYGASQPDPNIDHRRVPNLVTFLSRHGLTLTPSLSPERLACWQPGDIIITDYR
                     REGYETHIGFVSDKRNSQGVPYIIHHRPVYPVEEDSLAARPILGHYRYPKTE"
BASE COUNT         1542 a         1916 c         1973 g         1290 t
ORIGIN      
        1 gatcgaatca atccgggccg aggcagagcg ggcgattgcg gccgctccgg atgagaagga
       61 cctggaccag gtgcgggtga cctacctcgg ccgcaagggg cgcttgacgg ccatcctccg
      121 caacctcaaa gaagttcccg aaggccggcg ccctcaggtg ggacaggcgg ccaatgcggt
      181 gaaaaccttt ctcgaagggc tcatcgcgca aaaagaaacg gagttgaaaa cgagcgctcg
      241 gccagcggcg cccgctttcg actacacgct ccctggccgc ggccacgcgg tcgggaaact
      301 ccacctggtg acccagacca ccgacgccat cgtgcgcatc ttctcccgaa tgggtttcga
      361 caccgcctac gggcccgaag tggaactgga ctactacaat ttcgcagccc tgaactttcc
      421 cccggagcac ccggcccggg atatgcagga tactttctac gtttccgagg acgtggtgct
      481 gcgtacccac acctcgccgg tgcaggtgcg ggccatggaa aagcggcagc cgcctttgaa
      541 aattctctcg cccggccggg tttaccggca cgagtcgatt aacgtccgct cccacgttct
      601 gttccatcag gtggaaggtt ttgcggtgga caaagacatc acgttcggcg acatggaggg
      661 agtcctttcg gtcctggcct gggaactctt cggtcgggat gtcggtatcc gtttccgacc
      721 ctcgtttttc cctttcaccg agccctcggc tgaagttgat atttcctgca ccatctgcgg
      781 cggcaaaggc tgcggggtct gcaaaaatga gggctgggtg gaaattgccg gctgtgggat
      841 gatcgacccg gaagttttcc gctccgtcgg gtatgacagc gaactctaca ccggctacgc
      901 cttcggtctg ggggtggagc gggttgctca actcaagcac cggatcgatg acgtgcggct
      961 cttcttagaa aatgatttgc gctttctgcg gcaattctaa tgccaccgat tcgctgccct
     1021 cgtcccccga aagcaccaaa aaggaaagtg cgggaatccc cgggtaaacc gttaccactt
     1081 caagaatata cccgataaat catgaaagtt tcctacaact ggctccgcga actcattgac
     1141 ttcccctggg acccgaaaga attagccgag aagttgaccc tggccggcct ggaggtgggg
     1201 tcggtcacgc cgcagtaccc taacttagat ggagtggtgg tcgggcgaat cgagtcggtc
     1261 gagcgtcatc cccaggacga gcatctcttt tactgccagg tcaacatcgg ccgcgaaaga
     1321 cttgccattg tctccggcgc gcccaatacg gtttccgggg tgaaggtgcc cgtggcacca
     1381 cccgatacgg tgcttcccga cggcactctc gtgcagaaaa aaacgatccg aggagtcgaa
     1441 tctttcggga tggtctgctc cgagcgggag ctgggactct ccgacgatca ttccggtttg
     1501 atgattctgg acggtgactc cgaaatcggc cagcctttgg cacgcaccct gaacctgacc
     1561 gacgtgattc tcgatatcga tttgaccccc aaccgcgggg atgccctctc ccacatcggg
     1621 gtggcgcgcg acattgccgg gctcaccggc aatcggatca gacgccccaa aatcgaattc
     1681 aaagaaatcg aaaaaccggc tgcggcagca gtcgagatta ctctcgacga tccggtcggt
     1741 tgcccccgct actcagcccg ggtgttggcc aggatcaaag tcggccgttc cccctggtgg
     1801 ctccggcagc gggtggaagc ctgcggaatg cgcgccatca ataacgtcgt cgacgtcacc
     1861 aattacgtga tgctggagcg cggccacccg ctccacgcgt tcgactacga ccttttctcc
     1921 cggaagaaag tcgtggtccg gcgggctaag ccgaaggaaa agttcactac cttagacggg
     1981 ttcgagcgcg aattgaatga gaaggtactc ctcatcaccg atgacaaaat cccggtggcg
     2041 attgccggta ttatgggagg ggagaaaagc gaggtcgcgg agaaaacgac caacatcctt
     2101 ttggagagcg cctatttcga tccagtggtg atccgggtag gccgcaagtc gctcggcctg
     2161 cagaccgacg cctccaaccg ctttgagaga ggcaccgatc cgaacggact cctctacgcg
     2221 gtcaaccgcg ccgcggggct tctggcccgg ttggccggag ccgaagtttg cgccggcgtc
     2281 gtcgatgtct accccaagat catcgagccg gttatgctca ccctgcgccc cagccgcgtg
     2341 aataaaatct tgggcaccga gattccttct cccgaaatgg tgaccattct gaccaactta
     2401 gagttgggcg tcaccgccgg aaaagagctg caggtcaccg tgccgacttt ccgtccggat
     2461 gtcacccggg agatcgatct gattgaagaa gtcgctcgta tctggggtta tgagaaaatc
     2521 ccccggagcc attcggccag gggtgacgca tacgtaccga ctcacgccga agatgagctg
     2581 acgtcggcta tcaaaaactt tctctgcgcc cagggctaca acgaagtggt gaccaatacg
     2641 ctggtcgatc cgggctggct ggaaaaattg gggctgccgg cctcgcccgt gcggctgcaa
     2701 aaccccattg ccgaggcgct gtcggtcctg cgtaccggat tggtcagcag tctcctggag
     2761 gtggtgcggc gcaaccgtaa ccgccaggcc gatctgatca agctcttcga agtagggaga
     2821 atttttttgt ccagccggga gaaactgccc cgggaaaaac tggtgctggg aattgtcgct
     2881 accggtatcc ggcagcgcca gagctgggat gcggccgagg ctccggttga tctgttcgat
     2941 ctcaaaggtg ttctggattc tctggcgcag cgcttccgat tgaacgggtt tgcgctggag
     3001 cccaaagcag ttcccttcct cgatcccgaa agttcttttc gcctactttt gaacggcgca
     3061 gaagtcggtt cgctcggagc cgtcggatct gaagcgcaag agttttttga cctgagcgac
     3121 gcggtctact atgccgaatg tgactttggc gcccttttgg ccgcgggtgt ttcccggccc
     3181 acctacgagc cattggatcg ttacccgttc gtcgaacggg acatcgccgt ggtggttgac
     3241 cgaacctgcc tggccggaga cctcttggca gctattcgcc agaccggcgg agagctggta
     3301 cgggaggccc ggctgtttga cctttatcag ggtcagccgc tgccgctcga caaaaggaat
     3361 ctggccttcc ggctgcgcta tcaatctcgg gaaaggactt tgaccgaccg cgaggttgac
     3421 gctattcaag aaagaatcgt cgccgcattg aaaaaacaat ttgacgccgc gctgcggacc
     3481 tattaaattg tactttagga aatgcgggta agttattgta gaataaaaag atggcgcagg
     3541 acgtattaca aaaacttcag gaccgggtta atcaaacctt agaaacggtc gaaaaactaa
     3601 aacaggagaa ccagacactt tccgccggcc gacacgcgct gacggaacag ttggctctag
     3661 cccaaaaaaa tctgacggct ttgaccgtcc agtatcaaaa aatgcaaaag gaaaaggagc
     3721 gacaggacca ggactaccag aagaggaaag tggccctgcg gcagaagctg gaaactgttt
     3781 tgaccaaagt ggccaccgtg gaagagcgcc tgcgaaattt cacttgacag ccggcgccgg
     3841 tgtgattttc ttacacaccg tacttttgga gagggaagac agttacccaa ggaaagtttt
     3901 ttgagagaac ggcagaccta tctgtgacca gtgccagcac cccgaccgac cagaccaccc
     3961 tccgggtgaa catattcggt gaagagtacc cggtgcgggc caaggcggat ccggattaca
     4021 ttaccgcagt cgccaaatac gtggatcaga aaatgcggga catcgcccag aaaatcccga
     4081 accggtcatt cgccaacgtg gcggtgctgg ccgccctgaa cataaccgac gagttattcc
     4141 gcgaacgcca ggacaaagag aagaaactga ctgacgtcga agagaaaacc caggaaattt
     4201 tggtctggtt ggaccagaaa ctcgccgaag gccagattta gcgttccgtc tttcgctttc
     4261 cttcttgctt ccctcaataa acaactgagt tgctctccat tggggtagga cgcttgtgcc
     4321 ccggcggaag gagggaagat gacgaatttc attctcatcc tcattatcgc tgcggccgca
     4381 gtcgccgcag gcatcgcctt gggcttttgg ctctcccgtc gcatcggcgc ggagaaactg
     4441 gcccgggccg gggaactggc ccagcggatg atccgggagg ccgagaagga agccgagatc
     4501 aaaaagaaag aggcgctcct ggaggccaaa gacgagtggt accgcgccaa gcagaatttc
     4561 gagcgggagc tgcagaacaa aaagctggaa ttccagaaaa ccgaaaaacg atttcaggaa
     4621 cgggaagccg aagtcgcccg caaagtcgat ttcatcacca aaaaagaacg ggagctccag
     4681 aacagagaaa aagtgctttt ggcccgggag aaggggatca ctacccgcga aggagaactg
     4741 gagcggctgg tggtcgaaca gaaccgccag ctggaacggg tggcgcagat gaccaccgaa
     4801 gaggccaaaa agcagcttat ggaaaacttg atcaaccagg cccgggccga ggcggcacaa
     4861 accatcaagc agatgaaaga tgaggccgag cagaccggcg agcgcgaagc acgcaacatc
     4921 atcaccctgg caatccagcg ctgcgccgcc gaccataccg tcgaaaccac ggtttcagtg
     4981 gtcaatctcc cctctgacga aatgaagggc cgcatcatcg gccgcgaggg gcggaacatc
     5041 cgcgccttcg aagccgccac cggctgcgac gtgatcgtcg acgatacgcc ggaagcggtg
     5101 gtgctttccg gttttgaccc ggtgcggcgc gagatcgccc gcatggcgct ggaaaaatta
     5161 attttggacg gacgcatcca ccccgcgcgc atcgaggaag tggtgaccaa aacccagaag
     5221 gaaatggaga ccatcatccg cgaagcggga gagcaggcct gttttgagct gggaattcac
     5281 aacctgcact cagaagtggt caagaccctg ggaaggttga agttccgtac cagttacggc
     5341 cagaacgtcc tggcccacag ccaggaggtg gccatgctct gcggcctgat agcagcccag
     5401 ctgggtctcg atccgcaaat tgccaaacgc gccggccttc tgcacgacat cgacaaagcg
     5461 atcgaccgcg aaaccgaagg cacccacacc aagatcggct tgaacttcat gagcaagtac
     5521 aacgaggatc cggcggtctt gaacgccatt gcggcccacc acgaggacgt gatccaggag
     5581 acgccgtatg cggttctggt gcaggcggcg gatgccattt ccggttcgcg gcccggcgcc
     5641 cggcgggagt ctctggaagg ctacgtccgc cgtctggaaa aattggagga ggtggccgac
     5701 tccttcaaag gcgtggccaa agcctacgcc atccaggcgg ggcgggaagt gcgggtgatg
     5761 gtggaaaacg accaggtgga cgactccggc gcagcggaat tggccggcca gatcgccaag
     5821 aaaatacagg ccgaaatgga ataccccggg cagatcaaag tcaccgtcat ccgcgagatg
     5881 cgggccgtcg attacgcccg gtaacagtac gtattcaccc ggccgctgac ggacacaagt
     5941 acctctcgag gcaaaggcgt tcccggaggg aggggtttcc tcagtgaaga tttcgttcac
     6001 aggattctgg tttttgggat tattcggtac cctcgttgtg ctggagcgtg tcaaccttcc
     6061 aaagcgggaa tccagcttcg gtcagagatt gcttacagct gcccgggaat atctgcaaga
     6121 gaagccgacc tacgacttga gccggaggta taggaaaatc ggcttcccga agggcgacat
     6181 ccgccggaat aaaggtgtct gcgccgatct cgtcattcgg gtttaccggg aggtaggaat
     6241 cgatttacag gagcggatgc acgaggatat gaaaaagaat tttcatattt accttgcgcg
     6301 gcgtaactat ggagcaagcc agcccgaccc gaacatcgac caccgccggg tgccgaacct
     6361 ggtcactttt ctctctcgtc acggtctgac cttaactcct tctctctccc cggagcgtct
     6421 cgcctgctgg cagccggggg acatcatcat caccgattac cgccgcgagg ggtatgagac
     6481 ccacatcggt ttcgtctccg acaaacgaaa ttctcaggga gttccctaca tcattcacca
     6541 ccgtccggtc tatccggtgg aggaggactc tctggcggcc cggcccattt taggacatta
     6601 tcgctaccca aaaactgagt agcatcccca aagggttgga gcgaaaattt attgatttct
     6661 tgggtaaatg tgccgtttac gctgcggctt taagggcttc gaccagtttg gccaccgcgc
     6721 c
//
LOCUS       DBNGZD010000191         1558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_654454, whole genome shotgun sequence.
ACCESSION   DBNGZD010000191 DBNGZD010000000
VERSION     DBNGZD010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_654454"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..798
                     /locus_tag="AC16GM_05130"
     CDS             172..798
                     /locus_tag="AC16GM_05130"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR2923098.1"
                     /translation="MNFPHPDIHRYLMTVTPERDPIQKEMEEIAKKNNFPIIGPLVGR
                     FLCQMALLTKAKKILELGSGYGYSASWFAKGMPKGGTIICTEGSEENARMARDFWKRG
                     KVKVKLDFRVGDALSVIDKVKGQFDIILCDIDKWQYPEAFKKAVPRLRKGGLFITDNL
                     LWSGKVIEPNPDADTRGIIEFTKLIYNAKNLLATLLPIRDGISVCVKL"
     gene            814..1473
                     /gene="nth"
                     /locus_tag="AC16GM_05135"
     CDS             814..1473
                     /gene="nth"
                     /locus_tag="AC16GM_05135"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR2923099.1"
                     /translation="MSERLEALKQRTKKIIAGLKKEYPDAKCALNFSNPLELMVATIL
                     SAQCTDKRVNLVTPALFAQYRTPEDYFRAPREELEAAIRTTGFYRNKAKSIKACCLGL
                     LERFGGRVPNRMKDLLSLNGIGRKTANVILGTAYGIAEGVVVDTHVMRLSIRLGLTRE
                     KNPEKIEKNLIKIVPKKDWIIFAHLLILHGRNICIARKPKCLECVVNKWCPSAVKFMK
                     R"
     gene            1499..>1558
                     /locus_tag="AC16GM_05140"
     CDS             1499..>1558
                     /locus_tag="AC16GM_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR2923100.1"
                     /translation="MTSPLSRRDFLQALTAGLAL"
BASE COUNT          403 a          447 c          406 g          302 t
ORIGIN      
        1 cgtaccatcc cggagttact atcgaggaga ttaaaaaaga aaccggctgg gcgttaaagg
       61 taaatcagaa tctccatgct accgaaccgc cggacgaaac tgaactcaag attatccgga
      121 actacgatcc gcaccgcttc tggacgaagt agtgaacccg aaaggactgc tatgaacttc
      181 ccccaccccg acatccaccg ctacctgatg acggtcaccc ccgaacgcga tccgattcaa
      241 aaagaaatgg aagaaatcgc caaaaagaac aacttcccca tcattgggcc tttggtgggc
      301 cgcttcctct gccagatggc gctcttgacc aaagccaaaa aaattctgga actcggctcc
      361 ggctacggct actccgcctc ctggttcgcc aaggggatgc ccaaaggcgg cactatcatc
      421 tgcactgagg gcagtgaaga gaacgcccgc atggcgcggg acttctggaa acgtgggaag
      481 gtcaaggtca aattggactt tcgcgtcggc gacgccctct cggtcatcga caaggtgaaa
      541 gggcagttcg acatcatcct ctgcgacatc gacaaatggc agtacccgga ggcgttcaaa
      601 aaagcggtgc cgcggctgcg caaaggcggg cttttcatca ccgacaacct tctctggtcg
      661 gggaaggtga tcgagcccaa tcccgacgcc gatacgcggg ggatcatcga attcaccaaa
      721 ttgatttaca acgcgaagaa cctcttggcc acgcttctgc cgatccggga cggcatttcg
      781 gtctgcgtga aactgtaagg acagtccaag aaaatgtctg agcgcctgga ggcactgaaa
      841 cagcgaacta aaaagatcat cgccgggttg aagaaggaat atccggacgc taagtgcgcc
      901 ctgaacttct ccaatccatt agagctgatg gtggccacca ttctctccgc ccagtgcacc
      961 gacaaacggg tgaatctggt cacacccgcg ctctttgcgc agtaccgcac gccggaggac
     1021 tacttccgcg ccccgcggga agaattagag gcggcgatcc gcaccaccgg cttctaccgg
     1081 aataaagcca aaagtatcaa ggcctgctgt ctggggcttc tcgagcgctt cggcggtcga
     1141 gtaccgaacc ggatgaaaga tttgctctct ctgaatggca tcgggcgcaa gaccgccaac
     1201 gtcatcctgg gcactgccta cggcattgcc gaaggagtgg tggtcgatac ccacgtgatg
     1261 cggctttcca ttcgcctcgg cctgacgaga gagaaaaatc cggagaaaat tgaaaaaaat
     1321 ttgataaaaa tcgtaccgaa gaaggattgg ataattttcg cccacctttt gatcctccac
     1381 ggaaggaaca tctgcatcgc ccgcaagccg aagtgccttg aatgcgtggt gaacaaatgg
     1441 tgcccttccg ccgtaaaatt tatgaagaga tgaaaacccg tcgactctgg agaccctcat
     1501 gacttcccct ctttcccgcc gcgactttct gcaagcatta actgccggac tggccctg
//
LOCUS       DBNGZD010000192         2341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1419897, whole genome shotgun sequence.
ACCESSION   DBNGZD010000192 DBNGZD010000000
VERSION     DBNGZD010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1419897"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="AC16GM_05145"
     CDS             <1..931
                     /locus_tag="AC16GM_05145"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR2923101.1"
                     /translation="PGKRMRAKQSLPWKIIFSALLPWGALIWVGPLPGGAGVGGTELV
                     PVGDSTFRDSTRKGNIPADPSGADTLPTEIPPPLRMVENYSFGVGERLEYDITYGLFT
                     AGSAVMEVKELADVNGYPCYHVTSTARSNALVSKFFAVEDYVHSFVDARGVFTWRFEK
                     KIQEGKYKTHRQALYDQINHRVVTERNDTLAVAPFVQDALSALFFVRTQKLEVGKSLW
                     VEAFSDGKKYPLEVRVHKKEKIRVPAGEFRCIVVEPLLKTSGIFEHKGRLTVWLTDDR
                     LKLPVLMKSKVIVGNIAAELTAYTLGRPERFRR"
     gene            928..1911
                     /locus_tag="AC16GM_05150"
     CDS             928..1911
                     /locus_tag="AC16GM_05150"
                     /inference="COORDINATES: protein motif:HMM:NF052581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2086 family protein"
                     /protein_id="HIR2923102.1"
                     /translation="MKYEALDLAQVRTYSIRKRKHLARQTDFAGIPEPHDSIGQFLAI
                     LPQVLKAKNFVSLADTLAAAARKRPVIALLGAHVIKCGLSPVIIDLIRNGLLTHVAVT
                     GAGAIHDLEVAFFGQTSEEVAAHLQDGSFGMVRETAAIFNAAVAAGEGAGLGERLGYF
                     FLVRKPKFLRYSILAAAAEQKMPATVHVAFGTDTLHQHPGFDPASVGLLTHHDFKVLA
                     ASVGRLRPGAVVLNIGSAVVLPEVFLKALAVARNIEGARKVKGFTAANLDMIQHYRPN
                     VNVVGRPTLGGGTGYSFTGHHEIMLPLLAWAVKAKMQHTIGRTRNTGKSKR"
     gene            1955..>2341
                     /locus_tag="AC16GM_05155"
     CDS             1955..>2341
                     /locus_tag="AC16GM_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR2923103.1"
                     /translation="MPRLVECVPNFSEGRRPEVIDALQREIAAVPGVALLDREMDKDH
                     NRAVLTFIGEPEAVKKAAIRAIAKAAELIDMEEHKGEHPRIGATDVVPFVPISEVTMD
                     ECVQLAHQLGQEVGEKLGIPVYLYEAA"
BASE COUNT          489 a          665 c          735 g          452 t
ORIGIN      
        1 gccagggaaa agaatgcggg ctaaacagtc gctgccgtgg aagattattt tctccgcgct
       61 tcttccgtgg ggtgcgttga tctgggtggg gcctctgccg gggggggccg gggtcggtgg
      121 gactgagttg gtaccggtcg gagactcgac tttccgggac agcaccagaa aaggaaacat
      181 cccggccgac ccgtccgggg cggatacctt gccgaccgaa ataccccccc cgctccggat
      241 ggtggagaac tactccttcg gcgtggggga gcggctggaa tacgacatca cctacggact
      301 tttcactgcg gggtcggcgg tcatggaagt gaaagagctg gccgacgtca acggctaccc
      361 ctgctaccac gtcacctcca cggcgcgctc caacgccctg gtctccaaat tcttcgccgt
      421 ggaagactac gtccactcct ttgtggatgc ccgcggggtt ttcacctgga ggttcgaaaa
      481 gaagattcag gaagggaagt ataagaccca tcgtcaggca ctctatgatc agatcaatca
      541 ccgggtggta accgagcgca acgacacgct ggcggtggcg ccgttcgtgc aggatgcgct
      601 ctcggctttg ttcttcgtgc gcacccagaa gctggaagtg ggcaagtcgc tctgggtgga
      661 ggccttctcc gacggcaaga agtatccgct ggaagtccgg gtgcacaaga aagaaaagat
      721 aagggtaccg gccggggagt tccgctgcat cgtggtggag ccgcttttga aaacctccgg
      781 catcttcgag cacaaaggcc ggctcaccgt ctggctcacc gacgaccgct tgaagctgcc
      841 ggttttgatg aaaagcaaag tcattgttgg gaacatcgcc gccgagttga ccgcctacac
      901 cctgggcagg ccggagcgct tccgacgatg aagtacgagg cgctcgattt agcgcaggtt
      961 cgaacctact cgatccggaa gcgcaagcac ctggcccggc aaaccgattt tgccgggatc
     1021 ccggagccac acgactccat cgggcaattc ctggcaatcc tgccgcaggt tctgaaagcc
     1081 aagaattttg tgagcctggc ggacacgctg gccgcggccg cgcgcaagcg tccggtgatt
     1141 gcccttctcg gggcgcacgt gatcaaatgc ggtttatcgc cggtgatcat tgatctcatc
     1201 cgtaacggcc ttttgaccca cgtggcagtg accggggcgg gggcgatcca cgacttggag
     1261 gtggcgttct tcggccagac ctccgaagaa gttgccgccc atctgcaaga tggctccttc
     1321 ggcatggtgc gggaaactgc ggcgattttc aatgccgcgg tcgccgccgg cgagggagca
     1381 ggattgggag agcgcttggg gtatttcttt ctcgttcgga aaccaaagtt cctccgttac
     1441 agtatcctcg ccgccgcggc ggaacagaag atgccggcca cggtccacgt ggccttcggc
     1501 accgacacgc tccaccagca ccccggtttt gatccggcca gtgtcgggct tttgacccac
     1561 catgacttca aagtgctggc ggcctcggtc gggcgcttgc gccccggagc ggtggtgctc
     1621 aatattggct cggcggtggt gctgccggaa gtcttcctaa aagcgctggc ggtggcgcga
     1681 aacatcgagg gcgcccggaa agtgaaagga tttacggctg cgaatttaga tatgatccag
     1741 cattaccgcc ctaacgtcaa cgtcgtcggg cggcccactt tgggcggcgg caccggctac
     1801 tcgttcaccg gtcaccacga gattatgctg ccgcttttgg cttgggcagt caaagcgaaa
     1861 atgcagcaca caataggcag aacacgaaac acgggaaaaa gcaaaagatg aaatgcccca
     1921 ccccttctcc cggttcacgg cctaaggact ggaaatgccg cgcttagtgg aatgtgtgcc
     1981 caatttcagc gaaggccgca ggccggaagt catcgatgcg ctccagaggg agatcgcggc
     2041 agtgcccggc gtggcgcttt tggaccgcga aatggataaa gaccacaacc gggcggtttt
     2101 gactttcatc ggtgaaccgg aggcagtcaa aaaggcggct attcgggcaa ttgccaaagc
     2161 ggctgagtta atcgacatgg aagagcataa aggcgaacac ccacgaattg gcgccaccga
     2221 cgtcgtgccg ttcgtgccga tttccgaagt gacgatggat gaatgtgttc aactggcgca
     2281 ccaacttggc caggaagtgg gggagaagct gggcattccc gtttatctct acgaagcggc
     2341 g
//
LOCUS       DBNGZD010000193         2236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_239565, whole genome shotgun sequence.
ACCESSION   DBNGZD010000193 DBNGZD010000000
VERSION     DBNGZD010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_239565"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1108)
                     /gene="rsmH"
                     /locus_tag="AC16GM_05160"
     CDS             complement(182..1108)
                     /gene="rsmH"
                     /locus_tag="AC16GM_05160"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR2923104.1"
                     /translation="MSDFHVPVLVGEAVRYLVTDPNGIYCDCTVGGGGHARAVLAQLS
                     PRGRLIGLDRDREALAQIDRTAMEMRDRVTLVHCTFARLKDAVNQEGVTGLHGCIFDF
                     GVSLRQLEDERRGFSYLRDGPLDMRMDATQPKTAATVINQYSDKALALLFWHNADLSI
                     ARRLARLIVQARNQARITTTGQLCAAVLPVLPIRQRPGYLSRIFQAVRMEVNEEINEL
                     QQGLAQALDLLVPGGRLVTIAYHSGEDRQVKHLFQEKARGCICPPRFPRCQCGRKPTL
                     KILTRKAVVPAPEELARNPRARSAKLRAAEKI"
     gene            complement(1119..1589)
                     /gene="mraZ"
                     /locus_tag="AC16GM_05165"
     CDS             complement(1119..1589)
                     /gene="mraZ"
                     /locus_tag="AC16GM_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR2923105.1"
                     /translation="MTAFTGTFRFTLDHKGRVAIPAKFRRRARPARGKGPKARDGSGE
                     QFLLAVGFDGCLALYPPEEWQRVEEKLSGLSFTHRDFRYVNRLLHSRAAEVATDAQGR
                     ITIPQEMLAATGIDKEVVIIGASRWVEIWNPEKYQSYVASFGRTFEEVAEQLFT"
     gene            1895..>2236
                     /locus_tag="AC16GM_05170"
     CDS             1895..>2236
                     /locus_tag="AC16GM_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923106.1"
                     /translation="MEKWYKVISGLVALFLFGGLCFSNNAVAEETSFRFSFVSVDRNG
                     LNQTSAGVELPGDYLRMAEPGLPALPYRLVHLLLPAGMEVESLSVSCDAPQLLDGLFA
                     IPPAPERIAPDG"
BASE COUNT          451 a          687 c          610 g          488 t
ORIGIN      
        1 ctctgctctg tttgggttcg gctgagatcc tgttttaatt tttcgttctg cgccgcaaga
       61 cgaaacaccc acatccgctc ggccaccaaa aggctgccga acagtcccgc caaaagtaag
      121 atggccacgg gaaagcggat gaccggcact ctggcgcccg cgggcgtcgt cataagactc
      181 gtcagatttt ctccgccgcc cgcagtttgg cgctccgggc ccggggattg cgggccagct
      241 cttcgggcgc tggcaccact gctttgcgcg tcaaaatttt caatgtcggt ttccgcccgc
      301 actgacagcg ggggaaacgc ggcgggcaga tgcacccgcg ggctttctcc tggaacagat
      361 gtttgacctg ccggtcttca ccggaatggt aggcaatggt gaccaggcgc ccccccggaa
      421 ccaaaagatc aagtgcttga gccagcccct gctgcaactc attgatttcc tcattcactt
      481 ccatccgcac cgcctggaaa atgcgcgaaa gatagcccgg ccgctggcga atcggcagaa
      541 ccggcaggac cgcggcacac agttgccccg tagtggtgat gcgtgcctgg ttgcgcgcct
      601 ggacaatgag ccgggccagg cgccgggcga tcgacaggtc ggcattgtgc cagaagagga
      661 gggcgagcgc cttgtcacta tactggttga tcactgtcgc cgcagttttg ggttgggttg
      721 cgtccatccg catatccaga ggtccgtccc tcaggtagct gaagcctcgt ctctcatcct
      781 ctaactgccg caaactcacg ccaaaatcga agatgcagcc gtgcaacccg gtgacgccct
      841 cttggtttac cgcgtctttg agacgggcga aggtgcagtg taccagcgtc acccggtcgc
      901 gcatttccat tgccgttcgg tcgatctgcg ccagggcctc ccggtcccgg tcgagtccaa
      961 tcagtcgccc ccggggactc agttgtgcga gcacggcacg cgcatgccca cccccaccga
     1021 ccgtacagtc gcagtaaatc ccgttcggat cggtcaccaa gtaccggacc gcttccccga
     1081 ccaacaccgg aacgtgaaag tcgctcatcc cgcataggtt acgtaaaaag ctgttctgct
     1141 acttcttcga atgttcgtcc gaaactggcg acgtagcttt ggtacttctc cggattccag
     1201 atttccaccc agcgcgaagc gccgataatc accacttcct tgtcaatccc ggtcgccgcc
     1261 agcatctcct gcggaatagt gatccgtccc tgtgcgtcgg tggctacttc agccgcccgg
     1321 ctgtgcagaa gccggtttac gtaccgaaag tcgcggtggg taaaggagag accggaaagt
     1381 ttttcttcca cccgctgcca ctcctccggc gggtagagtg ccagacaccc gtcgaaaccc
     1441 acggcgagca gaaattgttc gccggagcca tccctggctt tcggcccttt tcctctggct
     1501 ggacgggctc gccggcgaaa cttggcgggg atggccaccc ggcctttgtg gtccaaagta
     1561 aagcgaaacg ttccggtaaa agctgtcata actcagccca aaatcaccac aattccccac
     1621 tattacccac aaaagtgggt tcaatgtagc cccccgccta aaggttgtca agaaaaaaat
     1681 gcaggtgagg gaaaaaatct ttggttgttg gttttacccc tcagttttgg tgggccgagc
     1741 gctcttttcc cctgcaagtt cagttcactt ttgctaacag cccaactgaa ccgaacttgc
     1801 acgcctgaaa aaaaggcctt gtgcgggaaa gggggcattg cctttgcagt ttcgccccct
     1861 tctggagtcg aaagactcac tagaggaggg gaaagtggaa aaatggtaca aagttataag
     1921 cgggcttgtt gcgctctttc tttttggtgg gctttgcttc agcaataatg ctgtggcaga
     1981 agaaacttct ttccgttttt catttgtgtc ggttgatcga aacggcttga accagacctc
     2041 ggccggagtc gaactgcccg gtgactacct gagaatggct gaacccggac tgcccgctct
     2101 cccctaccgg ctggtacatc tcttgttacc cgccgggatg gaagtcgaaa gtctctccgt
     2161 ttcctgcgac gcgccccaac tcctggacgg cctttttgca attccccccg cgcccgaacg
     2221 aatcgcgcct gacggt
//
LOCUS       DBNGZD010000194         5159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1558741, whole genome shotgun sequence.
ACCESSION   DBNGZD010000194 DBNGZD010000000
VERSION     DBNGZD010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1558741"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC16GM_05175"
     CDS             <1..539
                     /locus_tag="AC16GM_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR2923107.1"
                     /translation="FPSVPNPRPWSVCISTYRRSPGFAVPAGVKITGAYVNAALATTE
                     AKEKGFDEAILLDKDGFVCEGSAMNLFLVTDGKLVTPPLTSDILGGVTRDTIVTLANE
                     ELQMPVEESPVAPGQLFAAEEVFFCGTGVEVVPISSVGRRAIGDGKPGGVTGRLAALY
                     LQVVRGEKEKYQKWLVEI"
     gene            561..986
                     /locus_tag="AC16GM_05180"
     CDS             561..986
                     /locus_tag="AC16GM_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR2923108.1"
                     /translation="MRWVTREKVKVDRVACPWLIKEFIDPEAEFVFVPRDTDPASIRD
                     GIPFDMAGVELGHREGRCSFESILLKYGLSDPALHEMAKIIHAADIAADEDLVPEGKG
                     LKAIATGFSLLGLSDHQILEKEFIVYDALYRFCQEKVHK"
     gene            complement(1099..1677)
                     /locus_tag="AC16GM_05185"
     CDS             complement(1099..1677)
                     /locus_tag="AC16GM_05185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR2923109.1"
                     /translation="MGRTLSFGLTVLALFIVSATAQEKKEMAKPVEPTGLKVGDPAPD
                     FTLPYATKDTLVFQGGIKLSDFKGKKNVLVAFYPADWSGGCTKQVCSYRDNFSAFTDL
                     NCEVIMISGDYVFSHQEWAKHHNLPFILASDHDHSVAKTYGSFMPEMGFNKRTVFLVD
                     KAGIIRYINLKYNPGTPDDFEKLKAEAKKLAG"
     gene            complement(1706..3400)
                     /locus_tag="AC16GM_05190"
     CDS             complement(1706..3400)
                     /locus_tag="AC16GM_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923110.1"
                     /translation="MPALAADKEKLLYAFPPPPTPDAVEWPGSPLGAFNLITRTKGRT
                     QVHDKPTDAKPGKRDALVASAQRAIYRSFPNTILEHDVVIHGVRVRAITNSPHLYDFW
                     VDNWYSVREWETLTGQVPPHEPKIMVYALGGVSEEPEAAYYSRQTNTIIFFNTSYYGQ
                     LKSWVLGAVGRILAEEEGIHSIHGACVERNGKGILYIAPTGTGKSTSSYGLMNAGNCR
                     FHSDDWVYVRYAGHLRDGSLFVPMRILEEKRKVAEGFAVYRWLEANGKTKSDATLEGV
                     DLQNKVKRIRVGDLDFSRPLEAYAYTSEKVFYLRSNLVESFPEVAYHILHSKIENGPD
                     ATLDFVADQKSKIEGLYAQLERAQNPTVRRYFAQIDPARVKETLARLYAFDNTRAMLD
                     IARVFGPGNIFANPLEPVHLTTVMLLKRDFGDPLVLDWLSLDEFMTWLLIGETPDKKR
                     EIAFNAYRAVDDETEKKFLVKVQEANGGGIDPNFYGTYVSQPNIPPTLLQEFELFRVM
                     YQATNCYVMNTVLQKDPKIKGKQEAVQRSMALIARTIDRQPKDVHLTLENYVDFLA"
     gene            complement(3593..4003)
                     /locus_tag="AC16GM_05195"
     CDS             complement(3593..4003)
                     /locus_tag="AC16GM_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923111.1"
                     /translation="MKHNLISVKLITLTTFVFLFAGCGGSKNNALAPFQPQINNAPDN
                     FQFQATGIQKVTTTLQYTWQNSGTSATVNQACAITAGTATLVILDASSTQVYSRDLSA
                     NGTFATTAGTAGAWTIRVILSNLSGTLNFRVQKV"
     gene            4398..>5159
                     /locus_tag="AC16GM_05200"
     CDS             4398..>5159
                     /locus_tag="AC16GM_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923112.1"
                     /translation="MRTRGPKAGISIVGGLVFFLWVAAALQAQSPADTTTAEIKITTW
                     FLAGPVEGFLPVFGDTLKAIREKLPTTEYLDFDKLWPQAGKEIAWSPGQTITWQSFNS
                     PDGKPQFPIRSDAKSPHVAYAACYLASPRWQKVKLVVTTGNLFALYLDGEEKAKQTAV
                     SKAAKDTVETSAELVLTQGKHRLVIQTVFDPRDSLAFWSLSATFRVPKEKKVSAPQVS
                     LDPKHAPSLDDLVELESISELAISQDATKLAVVKRF"
BASE COUNT         1084 a         1350 c         1524 g         1201 t
ORIGIN      
        1 cgtttccatc ggttcccaac ccgcgcccct ggtcggtttg catttccact taccgccgga
       61 gtcccggctt tgcggtgccg gccggggtaa aaatcaccgg ggcgtatgtc aacgccgctc
      121 tggcgaccac cgaagcaaag gaaaagggct ttgacgaagc aattttgctg gacaaggacg
      181 gtttcgtctg tgagggctcg gcgatgaatc tcttcctggt caccgacggg aaattggtca
      241 cgccccctct gaccagcgat attctgggag gcgtgacgcg ggacacaatc gtcactttag
      301 cgaatgaaga gttacagatg ccggtggaag aaagtcccgt tgcgcccggg caactctttg
      361 cagccgagga agttttcttc tgcggcacgg gggtggaggt ggtgccgatt tcgagcgtgg
      421 gccggcgtgc cattggcgac ggaaaaccgg gtggcgttac gggccgctta gcggctctct
      481 acctgcaggt ggtgcgcggt gagaaagaaa aataccagaa gtggctggtt gagatttgac
      541 cgctacgagg atgaataatt atgcgctggg tcacgcgtga aaaggtgaaa gtcgaccggg
      601 tggcctgccc ctggctgatc aaggaattca tcgatccgga agccgagttc gtcttcgtcc
      661 cgcgcgatac cgatccggcg agcattaggg atgggattcc gttcgacatg gcgggggttg
      721 aactgggcca ccgcgagggc cggtgcagtt tcgaatcaat tctcctcaag tacggtctct
      781 ccgatccagc tctgcacgag atggcgaaaa tcatccacgc tgccgacatc gcggcggatg
      841 aggatttagt cccggagggg aaagggctga aggccattgc caccgggttc agtcttttgg
      901 gattatccga tcaccaaatt ttagagaaag aattcatcgt ctacgacgca ctctaccgct
      961 tttgccagga aaaagtgcac aaatgaattc cgttccggaa ctacgccgag gttagggtga
     1021 aacggcaaaa gcggggttgg tatcccccgc ctttgcattc ttaacgtggg gaagcggttt
     1081 tgtctttgga accggagttt acccggccag tttcttggct tccgctttca gtttttcaaa
     1141 atcgtccggc gtgccgggat tgtacttcag gttgatgtaa cggatgatgc cggctttatc
     1201 aacgaggaag accgtccgct tgttgaagcc catttccggc ataaaactcc cgtaagtttt
     1261 ggccacgctg tggtcgtggt cggaggccag gatgaagggg aggttgtggt gcttggccca
     1321 ctcctggtgg gagaagacgt agtcgccgga aatcatgatg acctcgcagt tcaggtcggt
     1381 gaaggcggag aaattgtcgc ggtaggagca gacctgcttg gtgcagccgc cgctccagtc
     1441 ggcggggtag aaggccacca gcacgttttt tttgcctttg aagtcggaaa gtttgatccc
     1501 gccctgaaat accagcgtgt ccttcgtcgc atagggcagc gtgaagtcgg gtgcggggtc
     1561 gccgactttc aagccggttg gttcaaccgg tttcgccatt tcctttttct cctgagcggt
     1621 ggccgataca atgaacagtg ccagaactgt caggccgaac gagagtgttc gtcccatatt
     1681 ccctcctttt tcggatgtcc atttttcacg ctaaaaagtc tacgtaattc tccagtgtta
     1741 agtgcacatc ttttggctgc cggtcgatgg tccgggcgat caaggccatg ctgcgctgca
     1801 ccgcctcctg tttccctttg atctttggat ccttctgtaa aaccgtattc atcacgtagc
     1861 agttcgtggc ctggtacatc acccggaaaa gttcaaactc ctgcaacagg gtgggaggaa
     1921 tgttgggctg gctgacgtac gtgccgtaga aattagggtc gattccgccg ccgttggcct
     1981 cctggacttt gacgaggaat tttttctcgg tctcatcgtc caccgcgcgg taagcgttga
     2041 aggcgatttc gcgcttttta tcgggcgttt cgccgattaa aagccaggtc ataaactcgt
     2101 ccagcgacag ccagtcgagc accagcgggt cgccgaaatc gcgcttgagc agcatcaccg
     2161 tggtgaggtg gaccggctct aagggattag cgaagatgtt cccgggacca aaaacccggg
     2221 caatatccag catggcacgg gtgttatcga acgcatagag ccgcgccagt gtttccttga
     2281 cgcgggcggg atcaatttgc gcaaagtatc gccgtacggt cgggttctgc gcccgttcca
     2341 gctgggcata gaggccttcg attttgcttt tctggtcggc gacgaaatca agagtagcat
     2401 ccggcccgtt ttcgatctta gagtgcagga tgtggtaagc cacctcgggg aagctctcca
     2461 ctaaattgga gcggaggtag aagactttct ccgaggtgta ggcgtaggcc tccagcggcc
     2521 gggagaaatc gaggtcgcct acccggatgc gtttgacctt gttctggaga tcgacgccct
     2581 cgagggtagc gtcacttttg gttttcccgt tggcctccag ccagcggtaa acggcaaatc
     2641 cttccgccac cttgcgtttt tcttccagaa tgcgcatcgg gacgaagaga ctgccgtcgc
     2701 ggaggtggcc ggcgtaacgg acgtagaccc aatcatcgga atgaaagcgg caattgccgg
     2761 cgttcatcaa accgtaggaa gaggtcgatt ttcccgttcc cgtgggcgcg atgtagagaa
     2821 ttcccttgcc gttccgctcc acgcaggcgc cgtgaatcga atgaatcccc tcctcttcgg
     2881 ctaggatccg acccaccgcg cccaagaccc aggacttcaa ttggccatag taagaggtgt
     2941 tgaagaagat gatagtgttc gtctgacggc tgtagtaggc ggcctccggc tcttcactga
     3001 cgccgcccag agcgtagacc ataatcttcg gttcgtgcgg cggaacctga ccggtcaggg
     3061 tttcccactc gcggaccgag taccagttgt cgacccaaaa gtcgtacagg tgcgggctgt
     3121 tggtgatggc gcgaacccga acgccgtgga tgaccacgtc gtgttccaga atggtattcg
     3181 ggaacgagcg gtagattgcc ctctgggccg atgccaccag cgcgtcccgt ttccccggtt
     3241 tggcgtctgt gggtttgtcg tggacctgcg tgcgcccttt ggtgcgggtg attaagttga
     3301 aagcacccaa aggggatccc ggccattcga ctgcgtcagg tgtggggggt ggcggaaaag
     3361 cgtagaggag tttttcttta tctgcggcca gtgcgggcac aacctgattt tctcaactcg
     3421 gttagcgtct ttgtttccgc agtacagaca tgaaacccag tataaaagcc aagagaagct
     3481 ttggcaagac ggcaaaaaaa ttgaactggg cggaagtata taaaatgaaa agaggttaaa
     3541 gtcgcccttt aacctcttcg tacaagcgaa gtgagaaaga tcagccgact gttcacacct
     3601 tctgtacccg gaagttcagc gttccgctga gattcgacag aatgacgcga atcgtccagg
     3661 cgcccgcggt gccggccgtg gtggcgaagg tgccgttggc gctgagatca cgggagtaga
     3721 cttgggtgct ggaggcgtcc agaatcacca gggtggcggt tcccgccgtg atggcacacg
     3781 cctggttcac ggtggcggag gtgccgctgt tctgccaggt gtactggagg gtggtagtca
     3841 ccttctgtat gccggtggcc tgaaactgga agttatccgg agcgttgttg atctggggct
     3901 gaaatggagc cagggcgtta ttcttactgc caccgcagcc ggcaaagaga aagacgaagg
     3961 ttgtcagggt aatcagtttg actgagatga ggttgtgttt cattctccag ctcctttcgt
     4021 gtatccggat tgcgtcgagc cccgtggtga tagagagaac ccgttgggta gtcgtttacg
     4081 acggctcaat acgacattgg gcattttcgg gctccttttc tcttgctgct ccatagaata
     4141 cgttccttcc caccatatat tgagcaaggg attacttatc ctgtcgaccg aaaggaaaaa
     4201 gtctgaagga atgcggacat tttccatcaa tttgtacctt atttggttcg agcttcgtac
     4261 ggagaaaacc cgagtggttg tcgcccgccc gcctctgcat atattcgaga ctgagaaacc
     4321 tatttatggt caccttgcga gcccttcgaa gacgcaaaat ggctgaacat cgtcaactca
     4381 ttaccaggag agcgagcgtg cgcacacgcg gtccaaaagc tggtatttct atcgtaggtg
     4441 gattggtctt ttttctctgg gtagccgcgg cgcttcaggc gcaatcaccg gcggatacca
     4501 ccactgctga gatcaagatt accacctggt ttctggccgg gccggtggag ggttttctgc
     4561 cggtgttcgg cgatacgctg aaggcaatcc gggagaaatt gccaaccaca gagtatctcg
     4621 actttgataa actctggcct caggcaggaa aagaaattgc ctggagtccg ggtcagacca
     4681 tcacgtggca atctttcaat tctcccgacg gaaaacctca gtttccaatc cgctctgacg
     4741 cgaaatcacc ccacgtggcc tacgccgcgt gttacctcgc cagcccgcgc tggcagaagg
     4801 tgaagttagt cgtcaccacc ggcaacctgt ttgcgctcta tttagacggg gaggagaaag
     4861 ctaagcagac cgcggtgtcg aaagccgcaa aggataccgt cgaaacatcc gccgaacttg
     4921 tcctcacgca agggaaacac cggctggtga ttcaaaccgt ctttgatccg cgcgattctt
     4981 tggccttctg gtcactctcc gccaccttcc gcgttccgaa ggaaaagaaa gtatctgcac
     5041 cgcaggtctc gcttgatccc aaacacgctc cctccctgga cgatctggtc gagctggagt
     5101 cgatttccga attagccatt tcccaggatg cgacgaagct ggccgtggtc aagcggttc
//
LOCUS       DBNGZD010000195         6758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1697957, whole genome shotgun sequence.
ACCESSION   DBNGZD010000195 DBNGZD010000000
VERSION     DBNGZD010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1697957"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..946)
                     /locus_tag="AC16GM_05205"
     CDS             complement(2..946)
                     /locus_tag="AC16GM_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923113.1"
                     /translation="MLAILVMSSLAQICQAQFFRPDVLHLTITPEIQDNPEDCRMWLV
                     ARVKNISREPAYVTPLDPDFGFLHIIVTNDKGDTLPIRVSASVFILRDTPELLQPQEE
                     LLDVWEIYPWFDLKPGMYKISSELTLVSNYKKPGQSATIRSNEVVMRVHRRTPEEEQV
                     QAATAATFEMFKERKTEAALKRCYEIMAQYPDNPFVINAYEMAASTYRNHLQLRDDPH
                     ARAELIKVTNDFFKKYPNSPLCDLFIYYREEQEKGKERKQFYEEVAALAPGTLTTRKS
                     CALPVPASLICRSLPKSFRSSPAFARSCSPASLWSSTS"
     gene            complement(975..1625)
                     /locus_tag="AC16GM_05210"
     CDS             complement(975..1625)
                     /locus_tag="AC16GM_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923114.1"
                     /translation="MKTEYQNWLDADLLKDSTVAKAIRDAFKQAKTDISFFQSFVRID
                     TLTFVDDVLFYQAYRDSFKTDKTNATYPYYLVSAQKLNVNAGGGFGIKAGVSFVPGPN
                     SPPFGNQRWSFVFYKDILANDALLPGEFWRYLAQVTIHELGHQRSGLTHANLDEVNCS
                     VAVVNHDDGGSGAKCVMNFGACITDAPFGVLQTLSFCNNKDTTKTNNCVYKLKHQF"
     gene            1988..4834
                     /locus_tag="AC16GM_05215"
     CDS             1988..4834
                     /locus_tag="AC16GM_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2923115.1"
                     /translation="MKQKDYIVATLTAGFIYFQAQAGGVMSATVDPALQVRLAQAHSS
                     DTLRVLLVLADRVPTAKLNSDLKTLTQSRTVRHKQVVEALQAKTRATQADLLGELERE
                     RERGTVAAYKGYWVDNIIWVRGEKGVVERYLNDPRVAEVYGEPQVQLVEPVGPGSQAP
                     LVPGGTEPGLRAIKADSVWLMGYTGRGRLVCNFDNGVLGTHHAFSTRWRGNNGAPASA
                     CWFDPTRNSTSPDTFQISTNWHGTFTMGIMCGRDPLTADTIGVAFGAQWIAAGVIDIS
                     SATSYLVLDAFEWVIDPDGDPNTIEDVPDVLNNSWGLPRFDGTCQPLDIGCDQVFWGA
                     IDNLEAAGCVVLFAAGNEGACGAASLRNPANRATTANNVFSVGATATTSPFTIASFSS
                     RGPSDCDGVSIKPEVVAPGLNVRSSGWPTGARTNASYGTSSGTSFSTPYVAGAVALLR
                     EYNPNLTVDTIKWALLNSALDRGTPGEDNTYGRGFINVKAALGLLPPNQGVHLFVKRD
                     ILTDLNGGTVEPGDTVDLLLRLGNSGADTSFGTWGRLFSTDSRISVVPGFDSAFFGTV
                     APKDSAANSGTLLRFAFDPTAPANINLPLQLPVFSNAGPADTLPLFFHTGDRLVRSIV
                     NHSLANNFFTVSNFGQYGFATGSLMPRSPGQGFFYPKAGGANNLFEMAFLVGTSDSTV
                     SNAVRGQPSDLIDRDFVPITGGNLIRQAPGPLANEETFCRFNDSGAIRPLGLEVNQRT
                     FAFSDTANDDYVILQYAVKNVSGQTRTGVRIGLFADWDFPYGSGASDSVNFDAGRNLG
                     YMFDGGNPSARKPRGVAVLTLPGATAYRAINSPTEIYDGFSQAEKWQGMSQGLITLRS
                     GGGDRSHLITTGPFDLFPGDSVKVAFAVVGGATFAQLQANADSAKVRYQRMNSSVPLL
                     TPPGLAGLAALLLAVLTFGFSWRRRAAAIPGEPL"
     gene            4975..6456
                     /gene="glgA"
                     /locus_tag="AC16GM_05220"
     CDS             4975..6456
                     /gene="glgA"
                     /locus_tag="AC16GM_05220"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR2923116.1"
                     /translation="MGLKVLFVSSEAVPFAKTGGLADVSGALPKALAALGCDVKVILP
                     RYKMAERAGFALEEAPLTRPVEVAVGSHHYPATLQRHRFYQNFEFLFVVNDHFYGRPD
                     LYRDPGTGFDYPDNDERFIFFARGTLEAVKQLGWQPDLIHCNDWQTALTPGYLKTVYR
                     NDPFFAQTRTLLTLHNVAYQGNFPKETFVKLGVDPGLFHPLGPFEFWGKVNFLKAGIV
                     YADLLSTVSERYAVEIQADAEFGYGLEGVLRQRNADLFGILNGVDYQEWNPATDQYIA
                     SRYTTKSLSKKKENKKALLKRCGWKATTLKQPLIGAISRLVDQKGFDLVADILEELLA
                     GGARFVLLGTGERRYHELFLRFAQTYPDRMKLFLGFDEPLAHLIEAGSDMFLMPSRYE
                     PCGLNQMYSLKYGTVPIVRETGGLADTVQEFSPGSANGAGTGFVFRHYDAGELLDAVR
                     RALKVYEDQTAWKRLMLAGMTKDYSWETSAKKYLSLYAKAQSR"
     gene            6508..>6758
                     /locus_tag="AC16GM_05225"
     CDS             6508..>6758
                     /locus_tag="AC16GM_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923117.1"
                     /translation="MKTNRRAPFLLGLALLLIAGTFLLQVRLFAFTQDDAFISYRYVQ
                     NFLAGNGLVFNPGERVEGYTNFFWVILLALFGRGGFDII"
BASE COUNT         1411 a         1852 c         1900 g         1595 t
ORIGIN      
        1 gtcagctcgt agaactccaa agcgacgctg gggagcatga acgggcgaac gccggcgaac
       61 tccgaaagct cttcggaagg gagcgacaaa tgagtgaggc tggaacggga agtgcgcacg
      121 actttctcgt cgtcagagtt ccgggagcta atgcggccac ttcttcatag aactgcttcc
      181 tctccttacc cttttcttgt tcttcccggt agtagataaa taaatcgcac aaaggagaat
      241 tggggtattt tttgaagaaa tcgtttgtaa ccttgataag ttctgcccgg gcatgcggat
      301 cgtcacgcag ctgcaggtgg ttacggtacg ttgaagcggc catttcatag gcgttgatga
      361 caaacggatt gtccggatat tgggccataa tctcgtagca tcgtttgagc gcagcctctg
      421 tcttgcgctc tttgaacatc tcaaaggtag ctgcggtagc tgcttgcacc tgttcctctt
      481 ccggagtacg tcgatgtacc ctcataacaa cctcgttcga ccttatggtc gccgattgtc
      541 cgggtttttt gtagttagaa accaaggtga gctcactcga aatcttgtac atccccggtt
      601 tcagatcgaa ccacgggtag atttcccata cgtcgagcag ctcttcttgc ggctgtaaaa
      661 gttctggagt atcccgcaat ataaacaccg atgccgaaac tcgaataggg agcgtgtcac
      721 ctttatcatt cgttactata atgtgaagaa agccgaaatc cggatccaac ggagttacgt
      781 aggcaggttc tcttgaaatg tttttgaccc gtgctacaag ccacattctg cagtcttcag
      841 gattatcttg tatttcaggt gtgatggtta aatggaggac atctggccgg aagaactgcg
      901 cttggcagat ctgcgctaag gaactcatga caagtatcgc aagcaatctc gttctcattg
      961 ttgcgctcag catttcagaa ttgatgtttt aatttgtaaa cgcagttgtt tgttttggtt
     1021 gtatccttat tgttacaaaa actaagagtt tgcagaactc cgaatggggc atccgttatg
     1081 cacgctccaa aattcatcac gcactttgcc ccactgccgc cgtcatcatg atttacaaca
     1141 gcaacgctac aatttacctc atccaaatta gcgtgagtaa gtccagacct ttgatgcccg
     1201 agctcgtgaa tggtaacctg tgctaggtag cgccagaatt caccgggcaa aagggcatcg
     1261 ttagctaaaa tatctttgta aaaaacgaaa ctccagcgtt ggttcccaaa gggtgggcta
     1321 ttagggcccg gtacgaatga aacgccggct ttaatgccaa aacctccacc tgcgttcaca
     1381 ttgagcttct gggcagagac aagataatag ggataagttg cattggtttt atcggtctta
     1441 aaagagtcac gataggcttg atagaataat acgtcatcca caaacgttaa ggtgtcaatt
     1501 cgcacaaaag attggaaaaa agaaatgtcg gtcttggctt gcttaaacgc gtcccgaata
     1561 gcttttgcga cagtgctgtc tttaaggaga tctgcgtcaa gccaattctg gtattcggtc
     1621 ttcatagaaa gagatgcaac attcggaggg ggaatgatat tcgggatacg tatcgtacaa
     1681 tgtaccgtaa gaaataggca gccagtcgtt atcattaggc accaaagaaa cttacgcttc
     1741 cccatgactg taatctaaca aaaaaaaaaa acggttttgt caaggatttt ttcatttttt
     1801 agacaatttt ctcttatttt tgttgaagtc attgcgggtc ttggacttga ctgctggtca
     1861 cagatgaagt cctggttttt acgtggaacg gtgccgtgtc ccccgggtat gtacaaacct
     1921 gcgccggact tgtatattgc ggtctttcga ttctggattc gctggtccta agaagatgga
     1981 gtaacctatg aaacagaaag attacatcgt tgctactctc acggcgggat ttatttactt
     2041 tcaggcacag gcgggcgggg taatgagcgc aacggttgat ccggctctcc aagtccgact
     2101 ggcacaggca cacagcagcg atacgctgcg cgtccttttg gtcttggctg acagagtgcc
     2161 gacggcgaaa cttaactccg atctcaaaac tttgactcaa agccggaccg tgcggcacaa
     2221 gcaggtggtt gaagcgttgc aagccaaaac ccgggcgacc caggcagatc tcctgggcga
     2281 attagagcgg gaaagagagc ggggtacggt cgctgcctac aaagggtact gggtcgacaa
     2341 cattatctgg gtgcggggag agaaaggggt cgtcgaaagg tatttgaatg atccgcgggt
     2401 ggcggaggta tacggcgagc cgcaggtgca attggtggag ccggtcgggc ccgggtcaca
     2461 ggctccctta gttcccggcg ggactgagcc ggggctgcgg gccatcaagg ctgactccgt
     2521 ctggctgatg ggttacactg gccgcggacg gttagtgtgc aatttcgata atggagtatt
     2581 agggacgcat cacgctttta gcacccgctg gcgggggaat aatggtgcac ctgcttctgc
     2641 ttgctggttc gaccctacgc gcaacagcac ctccccggac acctttcaaa tcagcaccaa
     2701 ctggcacggc acctttacga tgggaattat gtgcggccgc gatccgttga ccgccgacac
     2761 catcggtgtt gccttcggcg cgcagtggat cgccgccgga gtgatcgaca tcagcagtgc
     2821 gaccagttac ctggttttgg acgcctttga atgggtcatt gaccccgacg gtgatccgaa
     2881 cacgatagaa gacgtgcccg acgtgttgaa caattcctgg ggcttgcccc gctttgacgg
     2941 cacctgtcag ccgctggata tcggctgtga tcaggtcttc tggggagcga tcgacaacct
     3001 tgaggcggcg gggtgcgtgg tgctctttgc ggcggggaac gaaggggcct gcggagcggc
     3061 gtcgctccgg aatccggcga accgcgccac gaccgccaac aacgtttttt ccgtcggagc
     3121 caccgccaca acctcgcctt tcaccatcgc ctctttttcc agccgcggcc cctcggactg
     3181 tgacggggtc tccatcaaac ccgaagtggt ggcgcccggc ctcaatgtcc gctcctccgg
     3241 gtggccgacc ggagcccgca ccaatgcctc ctatggcact tcctccggaa cctctttctc
     3301 cactccctac gtggccgggg cggtggcgct gttgcgggag tacaatccga acctcacggt
     3361 agatacgatc aagtgggcgc ttttgaattc ggcgctagat cggggaacgc cgggcgagga
     3421 caatacctac gggcgcggat tcatcaacgt caaggccgcg ctgggtcttt tgccgccgaa
     3481 tcagggcgtg cacctctttg tcaaacgcga catcctgacc gatttgaacg gcgggacggt
     3541 ggaaccgggg gacacggttg atctcctgct acggctgggc aactcggggg cggacacctc
     3601 cttcggcacc tggggccggc ttttttcgac cgattcccgc atttcagtcg ttcctggttt
     3661 tgactctgcc tttttcggta ccgtggcgcc gaaagacagc gcggccaatt cgggaacgct
     3721 tctgcgcttc gcttttgacc cgacggcacc ggcaaatata aatcttccct tgcaactacc
     3781 cgttttctcg aacgccgggc cagcggatac cctgcccttg ttcttccata ccggggaccg
     3841 gctggtgcgc tcaattgtga accactcact ggctaacaac ttctttaccg tttccaattt
     3901 tgggcagtac ggctttgcta ccggttcact tatgccacgg agccctggcc aggggttttt
     3961 ctatccaaaa gcaggtggag cgaataacct ctttgagatg gctttcctgg tggggacgag
     4021 cgacagcacc gtctcgaacg ctgtgcgcgg ccagccctcc gatctcattg accgcgactt
     4081 tgtgccgatt acgggcggga atctgatccg ccaggctccg ggtcctttag ccaatgaaga
     4141 gacgttctgc cgcttcaatg acagcggggc gatccggcca ttggggttgg aggtcaacca
     4201 gcggaccttt gctttttcag atacggcgaa tgacgactac gtgattttgc agtatgccgt
     4261 caagaatgtt tccggccaga cgcggaccgg agtccggatc ggtctctttg ccgactggga
     4321 tttcccctac ggttcgggcg ccagcgattc ggtcaatttc gatgccggcc gcaacctggg
     4381 ctacatgttc gacgggggaa acccttcggc ccgcaagccc cgcggggtgg cggtcctcac
     4441 tctcccggga gcgaccgcct accgggccat taacagcccg accgagattt acgacggatt
     4501 ttctcaggca gaaaagtggc agggaatgag tcagggtctg attacgctcc gctcgggagg
     4561 gggcgaccgc tcccacctga ttaccaccgg tcctttcgat cttttccccg gtgattcggt
     4621 caaagtggct ttcgccgtcg tgggcggtgc gaccttcgcc cagttgcaag ccaatgccga
     4681 ttcggccaag gttcgctatc agaggatgaa ttcctcggtg cctctgctca ctcccccggg
     4741 tctggctggt ttggcggcgt tgcttttggc tgtccttact ttcggttttt cctggcggcg
     4801 gcgtgcagca gccatacccg gcgaacctct ttaagcgaat agtctcctcc gacgctcttc
     4861 cgtcggcaaa ttgttcttga ccgtccgccc gcaccgcata aattaccgga cgcacggtag
     4921 agcaggatac gatgttgctg actgagagat ttcacgaaaa atagaggagc atccatgggg
     4981 ctaaaagttc ttttcgtttc cagcgaggcg gtgccgtttg ccaagaccgg cggactggcc
     5041 gacgtttccg gcgccctgcc gaaggcctta gcggctttag ggtgcgacgt caaagtcatc
     5101 ctcccgcgct ataagatggc ggagcgggcc gggtttgctc tggaggaggc gcctttgacc
     5161 cgtccggtgg aagtggccgt cggttcccac cattaccccg ctaccctcca gcggcaccgg
     5221 ttctaccaga attttgaatt tctctttgtg gtgaacgacc acttctacgg ccggccggac
     5281 ttgtaccgcg atccgggaac tggctttgat taccccgaca atgatgaacg gttcatcttt
     5341 tttgcccggg ggacgctgga ggcggtcaag cagctgggct ggcagccgga cctcattcac
     5401 tgcaacgact ggcagacggc tttgactccc ggctacctca agacggtcta ccggaacgat
     5461 cctttctttg cgcagacgcg gacgcttttg accctgcaca acgtggccta ccaggggaat
     5521 ttcccaaaag agacgtttgt caaactgggc gtcgatccgg gcctgttcca ccccttaggt
     5581 ccgtttgagt tctgggggaa agtcaacttc ctcaaagcgg ggatcgtcta cgctgatctt
     5641 ctctccacgg tgagcgagcg gtacgcggtg gaaatccagg ccgacgctga gttcggatac
     5701 ggcctggaag gggtactgcg ccagcgcaac gccgacctct tcgggatctt gaacggagtc
     5761 gactaccagg agtggaatcc cgccaccgac cagtacatcg ccagccggta caccactaaa
     5821 tcgctttcca agaaaaagga gaacaagaag gcgctcctga agcggtgcgg ctggaaggcc
     5881 accacactca agcagccgct catcggtgcc atttcccggc tggtggacca gaagggattc
     5941 gacctggtgg ccgacatctt agaagagctt ttggccggag gagcgcgctt tgtgctcctg
     6001 ggtacgggtg agcggcgcta ccatgagctc ttcctgcgct tcgcccagac gtaccccgac
     6061 cggatgaaac tttttttggg gttcgacgaa ccgctggcgc acctgatcga ggccggcagc
     6121 gacatgtttt tgatgccctc ccgctacgag ccctgcggac tgaaccagat gtacagtttg
     6181 aagtacggga cggtgccaat tgtccgggaa accggcggac tggccgatac ggtgcaggaa
     6241 ttctcccccg gttccgccaa cggtgcgggg accggtttcg tgttccgcca ctacgatgcc
     6301 ggagagcttt tggacgccgt ccggcgggcg ctcaaagtct acgaagatca aacggcctgg
     6361 aagcggctga tgctggccgg catgaccaag gactactcct gggaaacttc ggcgaaaaag
     6421 tacctgagcc tgtacgcgaa agcccagagc cgctgaaccc ggtgccaccg tttcacgctc
     6481 tttcccggct ttaagcgttt ttctcttatg aaaacgaacc ggcgagcgcc tttccttttg
     6541 ggtctcgcac tcctcctcat cgccggcacg ttcctcctgc aggtgcggct ctttgccttt
     6601 actcaggacg acgctttcat ctcctaccgg tacgtccaaa attttctggc cgggaacggc
     6661 ttggtattca accccgggga acgggtggag gggtacacca attttttctg ggtcatcctt
     6721 ctggcccttt tcggccgcgg aggatttgac atcatttt
//
LOCUS       DBNGZD010000196         2907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_654871, whole genome shotgun sequence.
ACCESSION   DBNGZD010000196 DBNGZD010000000
VERSION     DBNGZD010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_654871"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2478)
                     /locus_tag="AC16GM_05230"
     CDS             complement(<1..2478)
                     /locus_tag="AC16GM_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR2923118.1"
                     /translation="MTHNSLTAAESIITSRSALVLPEHLPAHKSEAEGGCGVVGLAAS
                     VPVKGKHLLVPMLQMTNRGNGKGGGIAAVGLSPEQMGVSQEILDNAYLILVAYLNSSS
                     RQAVEERYIKPALDLHQAQKIPTLDDYREVKGLEVPPPEVWRYFGRVKPNVLREFTRK
                     NGLEKHAPQKVEDEFIYQNSFNLNREFYATGERRAFVLSHGRNLYVLKIVGYAQDVVR
                     YYRLEDLQAHIWIGHQRYPTKGKVWHPGGSHPFVGLDEALVHNGDFANYYSITEYLAQ
                     RNIAPLFLTDTEVSVLLFDLLNRVYDYPLEYLIEALAPTTERDFEMLPEEKKRIYQKI
                     QAAHIHGSPDGPWFFIIARNDRKSGAYQLLGITDTSMLRPQVFALQEGEAQIGIIGSE
                     KQAIDATLRSLQVEDSRFWSRADRYWNARGGSYTDGGAFIFSVTGNGNGTKNLICTDK
                     FSQQITLPRKEKPPAPIELSNGQKASPFSSDLALATDVRRLFEEIKGRLADLDYGAIA
                     SLCLDVAKFARKDDRSGTFAVELLTLLLDRVYPLGKKKRSFVLEIIENSLYEIFEVIP
                     ALGSAGQSTYRRIGREDRTTLRPPQAGETTLVCKAIGFAAEGTDSISQFVVEAIGPGW
                     KNLIIYALSGQRFIACGLGANMQGVTIDVYGSSGDYLASGLDGACVRMHGNAQDQLAN
                     IMKSGKLVVHGDVGQTFMYGAKGGEVYVLGNAAGRPLINATGRPRVVINGTCLDYLAE
                     SFMAGDPFQDGGFVIVNGLKYDDDGNLVELDTPYPGGNLFSLASGGAIYIRDPHQKVG
                     EDQLNGGKFTPVTAKDWELIEPYLKENQ"
     gene            complement(2468..>2907)
                     /locus_tag="AC16GM_05235"
     CDS             complement(2468..>2907)
                     /locus_tag="AC16GM_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR2923119.1"
                     /translation="EIAKKNIAMPIEYAIPKVHKFLEKEGIRDEIVVMASGGIRTAYD
                     VAKAIALGADGCIIGTAEIVAIGCTYCGNCERGRGCPVGITTTDPELSLLINPDWGAQ
                     RIVNLYRAWEIQWREILRKLALKSIRELRGRYDLLQHQHNHDA"
BASE COUNT          608 a          853 c          797 g          649 t
ORIGIN      
        1 ttgattttct ttgaggtacg gttcaatcaa ctcccagtct ttcgccgtga ccggggtgaa
       61 ctttccaccg ttcagttgat cctcccccac tttttggtgc ggatcgcgaa tgtaaatcgc
      121 cccgcccgag gcgagtgaaa agagattccc gccgggatag ggtgtatcta attcgacaag
      181 attgccgtcg tcatcatact taagcccgtt gacgatgacg aacccgccat cctggaacgg
      241 atcacccgcc atgaacgact cagccaaata atcgaggcag gtgccgttga tgacgacacg
      301 cgggcggccg gtggcgttga tgagcggtcg cccggcggca ttgcctaaaa cgtagacctc
      361 cccgcccttg gccccgtaca taaaagtctg ccccacgtcg ccatggacga ccaatttccc
      421 cgacttcata atgttcgcca actggtcctg cgcattgccg tgcatccgga cacaggcgcc
      481 gtccaagccg gaggcgaggt agtcgcccga actgccgtaa acgtcaatag taacaccctg
      541 catgttcgcc cccagcccgc aggcgatgaa ccgctgtccg gaaagggcgt agatgatgag
      601 atttttccaa cccgggccga tcgcttcaac tacgaactgt gaaatcgaat cggttccctc
      661 tgccgcaaac ccgatggcct tgcaaacaag tgtggtttcc ccggcctgcg gcggacgcaa
      721 tgtcgtgcgg tcttcccgac cgatgcgccg gtaagtactt tgacccgcac tgcccagcgc
      781 ggggattacc tcaaaaatct cgtagagtga attctcgata atttccaaga caaaactgcg
      841 cttcttcttc cccaagggat agacacggtc caaaaggagc gtcaataact caacggcaaa
      901 agtgcccgac cggtcatctt tccgggcaaa tttagccacg tcaagacaca aagaggcgat
      961 ggcgccataa tccagatccg ccaagcgccc tttgatctct tcaaatagtc gccggacatc
     1021 cgtcgcaagt gccaagtccg aagaaaaggg actggccttt tggccgttgg aaagttcaat
     1081 cggtgcaggc ggtttttcct tgcggggcag cgtaatctgc tggctaaatt tgtcggtgca
     1141 gatcaggttc ttggttccat tgccattgcc cgtaactgaa aagatgaacg cgcctccgtc
     1201 ggtgtaactg ccgccgcggg cgttccagta gcggtcggcc cgcgaccaga agcgcgagtc
     1261 ttcgacctgc aaactgcgga gcgtggcgtc aatcgcctgc ttctccgacc cgatgatgcc
     1321 tatctgcgcc tccccctcct gcaaggcgaa gacctgcggc ctgagcatgg aggtgtcggt
     1381 aatacccagg agttgatatg ccccgctctt gcggtcattc cgcgcaatga tgaaaaacca
     1441 ggggccgtcc ggcgagccgt gaatgtgcgc cgcctgaatt ttctggtaaa tccgtttctt
     1501 ctcctccggc agcatttcga aatcccgctc ggtcgtaggc gccagcgctt cgatcaaata
     1561 ttccagcggg taatcgtaga cccgattcaa caggtcgaac aaaagcacgg agacttcggt
     1621 gtcggtcaaa aagagcgggg cgatattgcg ctgagccaga tactcggtga tactgtagta
     1681 gttggcgaaa tcaccgttgt gtactaacgc ttcgtccaaa ccgacgaacg ggtgcgaacc
     1741 gcccggatgc cagacctttc ccttggtggg atagcgctgg tggccgatcc agatgtgggc
     1801 ctgcagatcc tccaacctgt agtagcgcac cacgtcctgc gcgtagccca cgattttcag
     1861 cacgtagagg ttccgcccgt gcgagagaac aaacgcccgc ctttctcctg tggcgtaaaa
     1921 ttcccgattg agattgaaac tgttctggta aatgaactcg tcttcaacct tttgaggtgc
     1981 gtgtttctcc agaccgttct tacgggtgaa ttcgcgcaga acgttcggtt tgactctgcc
     2041 aaagtagcgc cacacctccg gcggaggcac ttccaatcct tttacttcgc ggtagtcgtc
     2101 cagtgtcgga atcttctgcg cctggtggag gtcgagtgcc ggtttgatgt atcgctcttc
     2161 aaccgcctgc cgggaggaag aattgaggta cgcgaccaag atcaagtaag cattgtccaa
     2221 aatttcctgg ctgacgccca tttgctcggg cgaaagtcct acggcagcga tcccgccccc
     2281 tttgccgttt ccgcgattgg tcatctggag catcggaacc aataggtgct tgcctttcac
     2341 gggaacggag gcggctagcc cgactactcc gcatccgcct tcggcttcgc ttttgtgcgc
     2401 gggaagatgt tccggtaaaa cgagggcact gcgggaagtg attatcgatt cagcggcggt
     2461 cagggaatta tgcgtcatgg ttatgctggt gttgcagtaa gtcgtaccgg ccccgcagtt
     2521 cgcggatcga tttcaaagcc aatttgcgca gaatctcccg ccactggatt tcccacgcgc
     2581 ggtagagatt gacgatccgc tgcgctcccc agtcgggatt gatcaaaagt gacagttccg
     2641 gatcggtggt ggtgatgccc acggggcacc cccgcccccg ctcgcagttg ccgcagtagg
     2701 tgcagccgat ggccacgatt tccgccgtgc cgatgatgca gccgtccgcg cccaaagcaa
     2761 tcgctttggc cacgtcgtac gcggtgcgaa tgccgccgga agccatgact acaatctcgt
     2821 cgcggatgcc ttctttttcc agaaacttgt gcactttcgg aatggcgtac tcaatcggca
     2881 tggcgatatt cttcttggcg atttccg
//
LOCUS       DBNGZD010000197         3469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_725658, whole genome shotgun sequence.
ACCESSION   DBNGZD010000197 DBNGZD010000000
VERSION     DBNGZD010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_725658"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="AC16GM_05240"
     CDS             <1..933
                     /locus_tag="AC16GM_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2923120.1"
                     /translation="SKTFTGSIGVIEVAISNDSAMNGWVMSFLISSTTGGAFGKVDSV
                     QALPSLNPLGITLNLTKVNGVSPDSMWIQAVDPSGGSAPPGARRDALKIFMTNNRNPG
                     TYTLDTSRLFNKPTLRFTHPPTQISVPRFISGVETIFNDPPVLSCVPYRGVYFGAAVS
                     ILPEQASDPESDPLTFSLVSFSKTGGGGPPLNAPTVDASTGRFSWPTAADVAELGTWE
                     ATIAVTDLPIRPPLTCTVPIEVTDLRGDVNNDGLWSDLVDVIELINKVVFQIPITSEN
                     PNAADVNCNGVVDLEDIVALVNFVVFGAPAPCRG"
     gene            944..2230
                     /locus_tag="AC16GM_05245"
     CDS             944..2230
                     /locus_tag="AC16GM_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2923121.1"
                     /translation="MKRKYLGIAVLLLLFSSSASAGPFVDSVIVTSKTFSNGSQGIIE
                     VAIANDSAILGISLPLVITSGTGGAFGRVDSVRQLPSLLPFATNFSHLKVDGVNPDSL
                     AIISIDAFFSGGTVPPGSRRNAYLIFVTNNSNLGTYFLDSNRITVGSSLKFAHPPVQA
                     SVPAYIHGVETIANQAGPFADSLWITREGNCGPGQPCAIVGSLANDFSISGFQIGVEL
                     KSLSGDFYPGTFDSVKFGISLLPPPTISLAKVNGVSPDTLIILWTDITGFYPIPPGPR
                     REAVRLWVTNTGTSGCYQIALVDTRITHVGGGVNIPALSPPETLCAPQTAVRSEFTPG
                     LPRGFALGQNVPNPFNPTTVITFALPNEAHVSLEVFNILGQKVVTLVNEQVTAGLKSI
                     VWDGKDDQGWEIPSGIYFYRLQAGEFRETKRMVLLK"
     gene            2395..>3469
                     /locus_tag="AC16GM_05250"
     CDS             2395..>3469
                     /locus_tag="AC16GM_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2923122.1"
                     /translation="MTGVFSISAFGVDAPAVSKISPELLTRFQSGNPGATAKVWVFFT
                     DKGIADPAVLAKATADLSEYFSFRAIARRQKVRPGQLLFDETDLPVSADYLSLLSNSG
                     ITIHEVSRWLNAASISMTYAQALTVANLAFVREILPVMRTVQRLPAIEPVPRIEFAPA
                     APGALNYGPSFRQVSQIRANILHRLGYTGKGVLIGMLDTGFFIDHPVFDSLRLQGRIV
                     ATRDFINGDADVQDNAFDGQRDHGTATFSLLGGFADGTLIGTAFGASFALAKTEIVAT
                     EYIISEEDRWVKGLEWLDSLGADIVSSSLGYNVGDTGFSYTPAQMDGNTATTTKAADL
                     AASKGLLVVNAAGNEGNDPWRIII"
BASE COUNT          703 a          972 c          983 g          811 t
ORIGIN      
        1 tccaagactt tcactggcag cataggtgtc attgaagtgg ccatctccaa tgattccgcc
       61 atgaacgggt gggttatgtc cttcctaatc agttcgacca ccggcggggc tttcggcaag
      121 gtggactcgg tccaggcgtt gccttcactt aacccattgg ggataaccct taatctgact
      181 aaggtgaatg gcgtaagccc ggattccatg tggattcaag cggtagatcc gagcggcggt
      241 tcggctccgc ccggtgcccg gcgggacgct ttaaaaatct ttatgactaa caataggaac
      301 cccggtacct acacgcttga cactagccgg ctattcaata aacctaccct gaggttcacc
      361 catccaccga cgcagatcag tgttccccgc ttcattagcg gcgttgagac gattttcaat
      421 gaccctccgg tcttgagctg tgtcccgtat cgtggagttt actttggggc ggcggtatct
      481 attctgccgg agcaggcaag cgatccggag agtgatccgt tgacgttctc gctggtcagt
      541 ttcagcaaaa cgggaggtgg cgggccgcct ctgaatgcac caacagtcga cgccagcacg
      601 ggacgctttt cctggccgac cgccgccgat gtcgcagagc ttgggacgtg ggaggcgact
      661 atcgcggtta cggatttgcc tatcaggccg ccgcttacct gcacagttcc catagaggtg
      721 accgacctgc ggggggacgt gaacaatgac ggattatgga gtgatcttgt ggacgtcatt
      781 gaactgatca ataaggtagt gtttcagatt cccatcactt ccgagaatcc gaacgcggca
      841 gatgtgaact gcaatggggt ggtggattta gaggatattg tcgcgctcgt caacttcgtg
      901 gtgttcgggg caccggcgcc atgccgggga tagtgaggac ggtatgaaga ggaaatacct
      961 gggaattgcg gtactacttt tgctattcag ctcaagcgcg agcgccgggc cgtttgtcga
     1021 ttcagtgatt gtgacctcca agactttcag caatgggagc cagggcatta tcgaggtggc
     1081 cattgctaat gattcagcca tcttaggcat ttcgttgccc ctcgtcatca catcgggaac
     1141 aggtggggcg tttggaaggg ttgactcagt cagacagttg ccgtcgcttt tgccatttgc
     1201 taccaacttt tctcacctta aagtagatgg cgtgaatcca gattcccttg cgatcatctc
     1261 aattgacgca ttctttagtg gcggtacggt cccgcccggc tcccggcgaa atgcatacct
     1321 gatctttgtg accaataaca gcaacctcgg cacctacttt ttggattcca accggatcac
     1381 ggttggcagt agtctgaagt ttgctcaccc accagtacag gcaagtgttc cggcctacat
     1441 tcacggggtg gagacgatcg ccaatcaagc cgggccgttt gctgattcgc tgtggattac
     1501 gcgtgagggc aactgcgggc cgggccagcc ctgcgccata gtaggcagcc tggccaacga
     1561 tttcagcatc tcgggctttc agatcggcgt ggagttgaaa tcgctctcag gtgattttta
     1621 cccggggaca ttcgactcgg tcaagttcgg catttcactt ctccctccgc cgaccattag
     1681 cttggcgaaa gtcaatggcg tgagtccgga taccctgatc atcctttgga ccgatattac
     1741 cggtttttat cccatcccgc ccggcccccg gcgggaagca gtccgcctct gggtgacaaa
     1801 tacaggcacc agcggttgct accagatagc gctggttgat acccgaataa ctcacgtcgg
     1861 cggcggtgta aacatcccgg ccctgtcgcc tcccgagacc ctctgtgcgc cacagacagc
     1921 cgtgcggagt gaattcaccc ccggactgcc ccggggcttt gcgctggggc aaaatgtccc
     1981 gaatccgttc aacccgacga cggtgatcac ctttgccctg cctaacgaag cacacgtctc
     2041 gctggaagtt ttcaacattc tggggcagaa agtggtgact ctggtgaacg agcaggtgac
     2101 ggcgggattg aagtcgatcg tctgggacgg caaagacgat caggggtggg agatacccag
     2161 cgggatttac ttctaccgct tgcaggcggg tgagtttcgg gagaccaaac ggatggtgct
     2221 cctgaagtag tttatccgac ctttctttct gcgtcaagct gaccctctga gttttctctt
     2281 gcacaacggc gatttatttc gtaaaaattg ctgatgccct tctctgacca actctcccga
     2341 gaaaccgcac aaccatactt tcggcggtta cgcattattt tatgtgctct cggattgacc
     2401 ggcgttttct ctatttcagc tttcggcgtg gatgcacccg ccgtttcgaa aatctcaccc
     2461 gaacttctaa cgcggttcca gagcggaaat ccgggagcga cggccaaagt ctgggtcttt
     2521 ttcaccgaca agggaatcgc tgacccggcg gttctggcaa aggccaccgc tgacctgagc
     2581 gaatattttt cgttccgcgc catagcgcgg aggcagaagg ttcggcctgg ccagctgctc
     2641 ttcgatgaaa ccgatctgcc ggtttccgcc gactacctga gcctcctctc gaattccggc
     2701 atcaccattc acgaggtctc ccgttggctc aatgcggcca gcatctctat gacgtatgct
     2761 caagccctga cggtggcaaa tcttgcgttt gttcgggaaa tcctcccggt gatgcggacg
     2821 gtgcagcggt taccagcgat tgagccggtt ccgagaatcg aatttgcccc cgccgcgccg
     2881 ggagcgctga actacgggcc ctctttccgg caggtcagtc aaatccgggc gaatatcctc
     2941 caccggttgg gctacacggg caagggggtg ctcatcggca tgctcgatac cggatttttt
     3001 atcgaccacc cggtgttcga cagcctccgg ttgcaaggtc gtatcgtcgc cacccgcgat
     3061 ttcatcaacg gcgatgccga tgtccaagac aatgctttcg atggccagcg ggatcacggg
     3121 acagccacct tttcgctttt gggcggtttc gcggacggga cgctcatcgg cacggctttc
     3181 ggcgcttcat tcgctttggc caagaccgag attgtcgcca ccgaatacat catctccgag
     3241 gaagaccgct gggtgaaagg gttggagtgg ctggacagtc tgggagccga catcgtttcc
     3301 agttccttgg gctacaacgt gggagacacc ggcttcagtt ataccccggc gcaaatggac
     3361 gggaacaccg ccacgaccac aaaagcggct gacctggcgg cctccaaggg gcttctggtc
     3421 gtcaatgcgg cggggaatga agggaatgat ccttggcgca tcatcatcg
//
LOCUS       DBNGZD010000198         3865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_100761, whole genome shotgun sequence.
ACCESSION   DBNGZD010000198 DBNGZD010000000
VERSION     DBNGZD010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_100761"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /gene="rlmD"
                     /locus_tag="AC16GM_05255"
     CDS             <1..1003
                     /gene="rlmD"
                     /locus_tag="AC16GM_05255"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR2923123.1"
                     /translation="SPEVFFYRNKMEFSFFTDEAGKVCLGLHKSGTFDKMFDLEKCWL
                     QSELSNEIVAAVRDFAQRYELVPYHVRQHIGFLRFLAIRESKTNGQVLVNLVTNEGEF
                     PQAPDFVVTLTEKFPQITGIVRNISTSRANIATGEREELLWGKRYISEKISGLEFSIS
                     ANSFFQTNTKQAEMLFATTLAYADCTGSERALDLYSGTGTISLLLAQKVRHVTGIEVV
                     PEAVTMARLNAEHNGITNATFLSGEVRDLLPSQTRRGQFDLVIIDPPRAGLHEDVVRH
                     LIRLKVPNMVYVSCNPATLARDLKLFAAGGYKLIEVQPVDMFPHTPHIECVAKLTAD"
     gene            1017..1457
                     /locus_tag="AC16GM_05260"
     CDS             1017..1457
                     /locus_tag="AC16GM_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923124.1"
                     /translation="MTERTSDEKNRVFAFTLIFVSLFVTTCAKQKNKESALRETVTQY
                     ITTWQERNIEKIYEKVYELELPEYKARVSFENYKGHARLEEGLDSNTHYDFVLRNVEL
                     KGDTALVFIESHHLGTGSIQIDSLTAVFIGGRWYIPTYSSDVNQ"
     gene            1636..2154
                     /locus_tag="AC16GM_05265"
     CDS             1636..2154
                     /locus_tag="AC16GM_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923125.1"
                     /translation="MTGTQRSRFTPLAVFLGTASAVLLWAVAQPVARAQEHPSGHQEH
                     PSANHEKTAEVTQETLAEAIAGYIAKDATLKGGFFVVYDPQDKQAVALTLDKVHKDRL
                     SKVGKGTYFACADFKATDGKMYDLDVFMKESRAGLDVTEISIHKKEGKPRYDWMEENG
                     LWKQRPLVSTEK"
     gene            2166..3236
                     /locus_tag="AC16GM_05270"
     CDS             2166..3236
                     /locus_tag="AC16GM_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR2923126.1"
                     /translation="MHKRSRVRRTNLSASIVLPFVTGLILCGTFFLGSVITGGADNEP
                     SVPPPDRTDRTVECSYAFTVKIPPEARQVLLWAPLPKSNGHQEVSKLQVTSDYPYTFQ
                     TEKEYGNQILKMKAAGELPESLQVKISFVVARKAYRALGESDGNIDPISNRQIKRFLS
                     PDRLIPIDGKIAAEARQVVRDDMSGLEKARALYDHVVSTLKYDKTGTGWGRGDALYAC
                     DARRGNCSDFHSLFIGMARSVGIPARFVMGFPLPDGKTEGEIPGYHCWAEFYLDGVGW
                     VPVDASEASKHPEKRKLLFGGLDANRIEFTLGRDIRLASGMEPLNFFIYPLARVDGKK
                     DAAVNWNMRFRELSIEGAVRTE"
     gene            complement(3301..3753)
                     /locus_tag="AC16GM_05275"
     CDS             complement(3301..3753)
                     /locus_tag="AC16GM_05275"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR2923127.1"
                     /translation="MVRLKHIFLDGQVYFVTAVTHRRQKIFDKPPFARKLLTILYDLK
                     KQEKFLLFAFIIMPDHLHILLKPEDPLLSKLMQKIKGRSAWHINRLRQRNGKVWQDGY
                     YEHGIRNEKDFWEKYNYIFYNAVKAGLVEKPEDFFFSSAHPTNPVDGI"
BASE COUNT          953 a          986 c         1039 g          887 t
ORIGIN      
        1 ttcgccggag gttttcttct accgcaacaa aatggagttc tcgtttttca ccgatgaagc
       61 gggaaaagtt tgcctgggtc tgcacaaatc cgggacattc gataaaatgt tcgatttgga
      121 aaaatgctgg ctccagtcgg aactctccaa cgaaatcgtg gctgccgtcc gcgactttgc
      181 acaaaggtac gaacttgtcc cctaccacgt acgccagcac atcggcttct tgcgttttct
      241 cgcgattcga gaatccaaaa caaatggtca ggtgttagta aacttggtga ctaatgaagg
      301 cgagttcccg caggcaccgg attttgtggt gactctcacc gaaaaattcc cacaaataac
      361 cggcatcgta cgcaacatat ccacttcccg ggcgaacatc gccaccggcg agcgggagga
      421 actcctctgg ggcaagcggt atatttccga gaaaatttcc gggctggagt tctccatctc
      481 ggccaattct ttttttcaga ccaataccaa gcaggccgaa atgcttttcg ccacgacgct
      541 ggcgtacgcc gattgtaccg gcagtgaacg cgccctcgac ctctacagtg gcaccggaac
      601 gatttcgctg ctcttggcgc aaaaggtacg gcacgttacc ggcattgagg tggttccgga
      661 agcggtgacc atggcccggc tgaacgcgga acacaatggg attacgaacg caacctttct
      721 ctccggcgaa gtgcgcgatc ttttgccatc acaaacccgt cggggacagt tcgacttggt
      781 gattatcgac ccgccccgcg ccggcctgca cgaagatgtc gtccgccacc tcattcgcct
      841 gaaagtcccc aatatggttt acgtctcctg caatccggca accttggcgc gcgacctgaa
      901 gctctttgcc gccggtggtt acaagttgat tgaggttcaa ccggtagata tgtttccgca
      961 cacgccgcac atcgaatgcg tggctaaact gacagcggat taggaagttc aaacagatga
     1021 cagagcgaac ttcagatgaa aaaaataggg tttttgcctt tacgcttatt ttcgtttccc
     1081 ttttcgttac tacctgcgct aaacaaaaaa ataaagaatc cgctttgcgg gagaccgtta
     1141 cacaatacat aaccacctgg caagagagga acatagaaaa gatttatgaa aaggtatatg
     1201 agttagaact ccccgaatat aaagctcggg tttcattcga gaattacaag ggacatgcgc
     1261 ggcttgaaga gggattggat tctaatactc attatgattt cgttttaaga aacgtggaac
     1321 ttaagggaga cactgctttg gtattcatag aatcccatca tttaggaaca gggagcattc
     1381 aaatagattc actaaccgca gtgttcatag gggggcgttg gtatattccc acttattctt
     1441 ccgatgtcaa ccagtagcaa aaaagagagt gaagttgttc gacatagatg ggagatcaag
     1501 tgggtcaatc gatcgttttt cggatcactc aaagcttttg gcccgcttcc tcttctcctg
     1561 tcagtcccct tatggaaact ctattacttc cgtagaggtt gacgtataaa agacaacaac
     1621 cgaaaggagg cagaaatgac cggaacacaa cgttcacgtt tcaccccgct ggcagttttc
     1681 ctcggcaccg ctagcgcagt tcttttgtgg gccgtagcgc agccggtggc gcgcgcgcag
     1741 gaacacccca gcggccatca ggaacatccc agcgccaatc acgaaaagac ggcggaggtg
     1801 acccaagaga ctctggcgga agccattgcg gggtacattg ccaaggacgc aaccctcaag
     1861 ggcggctttt tcgtcgtcta cgatccacag gacaagcaag cggtcgcgct cacgctggac
     1921 aaagtgcaca aggaccgcct ttcaaaagtg ggaaagggga cctacttcgc ttgcgctgac
     1981 ttcaaggcca ccgacggcaa gatgtacgat ctggacgttt tcatgaaaga atccagagcc
     2041 gggctggatg ttaccgaaat ttccatccac aagaaggaag ggaaaccacg ctacgattgg
     2101 atggaggaaa acggcttgtg gaaacagaga ccgcttgtta gcacggaaaa ataagcgctg
     2161 tcagcatgca caagcgtagc cgagtccggc gcacaaatct gtcggcgtct atcgttttgc
     2221 cgtttgtgac cgggctcata ctctgcggaa cgttttttct cggcagcgtc atcaccggcg
     2281 gtgcagataa cgagccgagt gttcctccgc cggaccgaac agaccggacg gtggaatgct
     2341 cctatgcctt caccgtcaag attcccccgg aagcccggca agtcctactc tgggcgccgc
     2401 tcccaaaatc gaatggtcat caggaagtct ccaaactgca ggtgaccagc gattacccgt
     2461 acaccttcca gaccgaaaaa gaatatggga atcaaattct gaagatgaaa gcggcggggg
     2521 aactgccgga gagtttgcag gtcaaaatat ctttcgtggt ggctcgcaag gcctaccggg
     2581 ctctgggcga gtcggacgga aatatcgatc ccatcagcaa ccggcagatc aagcgtttcc
     2641 tttctcccga ccgcctcatc ccgatcgacg gcaaaatcgc cgctgaggcg cggcaggtgg
     2701 tgcgtgacga tatgtccggc ttagagaaag cccgcgcgct gtacgaccac gtggtttcca
     2761 ccttgaagta tgataaaacc ggaaccggct ggggacgggg tgatgcgctc tacgcctgcg
     2821 atgcgcggcg ggggaactgc tccgatttcc attcgctttt catcgggatg gcccgctccg
     2881 ttggcattcc ggctcggttc gtgatgggct ttcccctgcc cgacggaaaa actgaaggcg
     2941 aaatccccgg ctaccactgc tgggcggagt tctacctgga tggtgtgggc tgggtaccag
     3001 tggatgcctc ggaagcgagc aagcatccgg agaaacggaa actactcttc ggtggactgg
     3061 atgcgaatcg gatcgaattc actttgggac gggatattcg tctcgcttcc gggatggagc
     3121 cgctgaactt cttcatttat cccctggccc gggtggatgg gaaaaaagat gcggccgtca
     3181 actggaacat gcgatttcgg gaattgtcga tagaaggggc ggtaagaacg gagtgagaag
     3241 gatttttccc gcgtaggcgg gtcgatacga ctcgtaccga ggctattagc cccgctgatt
     3301 ttatatccca tccaccggat tggtcgggtg tgccgatgaa aagaaaaaat cttccggttt
     3361 ttctaccaac cccgctttca ctgcattata gaagatgtag ttatacttct cccagaagtc
     3421 tttttcgttt cttattccat gttcgtagta tccatcctgc cagaccttgc cgttgcgttg
     3481 ccgtagtcgg ttgatgtgcc aggctgagcg acccttgatc ttttgcataa gtttcgacaa
     3541 aagtgggtct tcaggcttga ggagaatatg aagatgatcc ggcatgatga taaaagcgaa
     3601 gaggagaaat ttttcttgtt ttttaagatc gtagaggatg gtaaggagtt ttcgggcaaa
     3661 cggtggtttg tcgaagattt tctggcgtcg gtgggtgacg gccgttacga agtaaacctg
     3721 accatcaagg aagatgtgct ttagacgtac cacgaatgga gaatatatga aaaggcggca
     3781 ctaagagtgc cgcctacgcg catagagcac ggtctccttt ctgcgtaggc ggggctctta
     3841 gccccgccag cccgtcaacc aaaag
//
LOCUS       DBNGZD010000199         3025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_655179, whole genome shotgun sequence.
ACCESSION   DBNGZD010000199 DBNGZD010000000
VERSION     DBNGZD010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_655179"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="rplJ"
                     /locus_tag="AC16GM_05280"
     CDS             <1..332
                     /gene="rplJ"
                     /locus_tag="AC16GM_05280"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR2923128.1"
                     /translation="TGLEGLAEHLSGPTGVVFGADDPVVAAKILFEFIKKAEKPRLKA
                     FWVTGQKYARADFNRLAQLPSRPELLARVVGVLNAPLANLVSTFDGILRTFIGTIEAI
                     AEKKQKL"
     gene            431..811
                     /gene="rplL"
                     /locus_tag="AC16GM_05285"
     CDS             431..811
                     /gene="rplL"
                     /locus_tag="AC16GM_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR2923129.1"
                     /translation="MAEAKVAELVGQIESLTVLELAELSQTLQEKFGVSAAVPMAAPA
                     GGGAAPAAAIEEKTEFSVVLAGTGDKKIQVIKVIRELTGLGLKEAKDLVDGAPKVVKE
                     GIPKEEAETIRTKLQEAGATVEIK"
     gene            1012..>3025
                     /gene="rpoB"
                     /locus_tag="AC16GM_05290"
     CDS             1012..>3025
                     /gene="rpoB"
                     /locus_tag="AC16GM_05290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR2923130.1"
                     /translation="MAQATIERKSYGRIDTVVEMPNLLDVQLGSFNDFLQMDVPPAER
                     KHVGLQQIFQEIFPISDVHENYALEFVKYQVGTPRYSIAECQERNMTYAAPLKATLRL
                     MTKGGEGKEKRVRDIIEQDVFLGEIPLITEKGTFIVNGAERVIVSQLHRSPGVFFDEE
                     VHPNGKKLFSARIIPYRGSWVEFNVDVNDLLYCHIDSRRKLAGTTLLRALGYATDEAI
                     TSLFYETDEEDLSGRKGSKAVGKISAASVVDQATGEIILQAGDPITEEILEKIKQAKI
                     ESLRVIKSDLSRDAGVILNTLKKDAAHSQEEALLKIYALIRPGEPPTPEVAFGVLERL
                     FFNAKRYDLGKVGRYMIDERLNLDIPLDCTVLDKRDFIAIIKYLLDLRNGLGDVDDID
                     HLGNRRTRTVGELLANQFSVGLSRMARTMRERMSLKDTDSITPHDLVNARTVSAVIDA
                     FFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLTRERAGFEVRDVHHTHYGRICPIE
                     TPEGPNIGLIASLSTYVRINQFGFLETPYRKAKNGKVTDEVVFLTADQEDRHVTAQAN
                     EPLAKDGRFLNEYVKARHRGDFPVVPASQIDYMDVSPKQLVSVAAALIPFLEHDDANR
                     ALMGSNMQRQAVPLLQTEAPVVGTGVERKAALDSGAVVLARRAGVVE"
BASE COUNT          673 a          917 c          904 g          531 t
ORIGIN      
        1 agaccggctt ggagggcttg gccgaacacc tgtccgggcc gactggggtc gttttcggtg
       61 ccgacgaccc ggtggtggcc gccaaaatcc tcttcgaatt catcaagaag gcggaaaagc
      121 cgcgcttgaa ggctttctgg gtgaccggtc aaaaatatgc ccgggcagat ttcaatcgct
      181 tagcgcagct gccgagccgc ccggaacttt tagcgcgggt ggtcggggtc ttgaacgccc
      241 cactggcaaa tctcgtcagc acgttcgacg gtatcctgcg gacctttatc ggaaccattg
      301 aggcgattgc cgaaaaaaaa cagaagctgt aagtcgaggc cagcaacgga actcactcct
      361 gcaggttcgc tgccgggccg aaagcgagaa ataacccgat aaaaagagaa aaacgagaag
      421 aggagagacg atggcagaag cgaaagtagc tgaactggtc ggtcagatcg agagcctgac
      481 cgttctggaa ttggcggagc tctcgcagac gctccaggaa aagttcggcg tttccgccgc
      541 cgtgcccatg gcggcgcccg caggcggcgg tgcggcgccg gccgcggcga tcgaggagaa
      601 aacggagttt tccgtggttc tggccggaac cggcgacaag aaaattcagg tgattaaggt
      661 gatccgggaa ctgaccggcc tgggtctcaa ggaagccaaa gatctggtgg acggagcgcc
      721 gaaagtggtc aaggaaggaa ttcccaagga agaagcggaa acgatccgga ccaagctgca
      781 agaggccgga gccacggttg agataaagta agaattgccg aagaatcccc ttaggaacgg
      841 tccgggtgcc cgtacgggcc gcggacggag ccgaacggga atcggtgtct ccgctgctat
      901 tcgtgttcag aaacgaacag taccactttc gtataagcca gggcgggaaa actccggcgc
      961 acccggggga aaagcccgtc tatccctatt atccgaagag gagtgaggcc tgtggcgcaa
     1021 gcaaccattg agcgcaagtc gtacgggcgg atcgataccg tcgtcgagat gccgaacctc
     1081 ctggacgtgc agctgggttc gttcaacgat tttctccaga tggacgtgcc gccggcggag
     1141 cgcaagcacg tcggcctgca gcagattttc caggaaatct tccccatttc cgacgtgcac
     1201 gagaactacg ctttagaatt cgtcaagtac caggtgggca cgccgcgcta ctccatcgcc
     1261 gagtgccagg agcggaacat gacttatgcg gccccgctca aggccactct ccggcttatg
     1321 accaaaggcg gtgaggggaa ggagaagcgg gtccgggaca tcatcgagca ggacgtgttt
     1381 ctgggtgaaa tccccctcat taccgagaag ggaaccttca tcgtcaacgg ggccgagcgg
     1441 gtcatcgtct cgcagctcca ccgctcgccc ggagttttct tcgacgagga agtccacccc
     1501 aacgggaaaa aactcttttc ggcccggatc attccctacc gcggctcgtg ggttgagttc
     1561 aacgttgacg taaacgacct cctctactgc cacatcgact cacgccggaa gctcgccggc
     1621 acgacgctcc tgcgggccct gggctacgcc accgacgagg ccatcacgag tctcttctac
     1681 gaaaccgacg aagaggacct ctccggccgc aagggctcca aggccgtcgg caaaatatcc
     1741 gccgcgagcg tcgttgacca agctaccggt gaaatcatcc tccaggcggg cgacccgatc
     1801 accgaagaga ttttagagaa aatcaagcag gccaaaattg agagtctccg cgtcatcaaa
     1861 agcgacctct cccgcgacgc tggcgtgatc ctcaacacct tgaagaaaga tgctgcccac
     1921 tcgcaggaag aagcactctt aaagatttat gcattgatcc ggcccggcga accgccgacg
     1981 ccggaggtgg cgttcggcgt cttagaacgg ctcttcttca atgccaagcg ctacgatctg
     2041 ggcaaggttg ggcgctacat gatcgacgag cgcttaaacc tcgacattcc cctggactgc
     2101 accgttctgg acaagcgcga cttcatcgcc atcatcaaat acctcctgga cctgcgcaac
     2161 ggcttgggcg acgtggacga catcgaccac ctgggcaatc ggcgcacccg gacggtgggg
     2221 gaacttttag ccaaccagtt ctcggtggga ctttcgcgga tggcccggac catgcgggaa
     2281 cggatgtcgc tcaaagacac cgattccatc accccccacg acctggtgaa cgcccgcacc
     2341 gtctcggcgg tcatcgacgc cttcttcggc tccagccagc tctcgcagtt catggaccag
     2401 accaatccct tggcggagct gacccacaaa cggcgtcttt cggctctggg accgggtgga
     2461 ctcacccggg agcgcgcggg cttcgaagtc cgcgacgtcc accacaccca ttacggccgg
     2521 atctgcccga tcgaaacgcc cgaaggtccc aacatcggcc tcatcgcttc cctttccacc
     2581 tacgtacgca tcaaccagtt cgggttcctc gaaacgccgt accgcaaggc gaagaacggc
     2641 aaggtcaccg acgaagtggt gtttctcacc gccgatcagg aagaccgcca cgtcaccgcc
     2701 caggccaatg agcctttggc caaagacggc cgtttcttga acgaatacgt caaggcccgc
     2761 catcggggtg acttcccggt ggtgccggcc agtcagatcg actacatgga cgtctcgccc
     2821 aagcagctcg tgtcggtggc cgccgccttg attccctttc tggagcacga cgacgccaac
     2881 cgggcgctca tgggctccaa catgcagcgc caggcggtgc cccttttgca aaccgaagcg
     2941 ccggtggtgg gcaccggggt ggagcgcaaa gcggctctgg attccggcgc agtggttttg
     3001 gcccggcgcg ccggggtggt tgaat
//
LOCUS       DBNGZD010000200        14884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_101024, whole genome shotgun sequence.
ACCESSION   DBNGZD010000200 DBNGZD010000000
VERSION     DBNGZD010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_101024"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="AC16GM_05295"
     CDS             complement(<1..1415)
                     /locus_tag="AC16GM_05295"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR2923131.1"
                     /translation="MKRIQLARKFLSWAVLFIMTAPFPASADRYKPFGDELSTRHWPP
                     DRSYDLQHIRLDLTFDWDQKKVSGKVTYRLVPLNSGFREVRLNQGDLNITGVTDAAQK
                     SLVYAADDSFLVVKLPEAYDAGKPIEFSVAYWAKPRKGLYFVGPDSGYPTKPKQIWSQ
                     GEDEDNHFWFPCWDFPNDRATSEMVATVPETWTVVSNGRLVEVKPNPAQKTKTFHYSQ
                     EQPHVTYLISVVAGEFEKYADDFDGTPVEYYVPLGTGEAKARRSFGITPDAVKFFSEK
                     IGFRYPYAKYAQSAVTDFIYGGMENISATTQTDRTLHAEVSEPVVSSEGLVAHELAHQ
                     WWGDLVTCNEWAHAWLNEGFATYFASLYTEHHRGKDEFQYEMLNHARAYISEDENEYR
                     RPIVDNWYTDSDDLFDSHLYPKGGWVLHMLRGMLGDNLFWKGINNYAKTYAYQTVTTR
                     EFQKVMEEATGRSLGWFFDQWV"
     gene            complement(1412..1897)
                     /locus_tag="AC16GM_05300"
     CDS             complement(1412..1897)
                     /locus_tag="AC16GM_05300"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR2923132.1"
                     /translation="MAVYIISDTHIPDRVKAIPKKFLERLTPEDIVLHAGDFTTPVVL
                     EELRRRAKVHAVWGNMDGPEVRKELPRKTVVTLGKVKIGLYHGHGAPEGLVDRVRAEF
                     TEPVDIIVFGHSHTPFSKKFGATLLFNPGSLSINFDRRTLTYGRLQTDPELHIELVEI
                     P"
     gene            complement(2071..2355)
                     /locus_tag="AC16GM_05305"
     CDS             complement(2071..2355)
                     /locus_tag="AC16GM_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR2923133.1"
                     /translation="MTKADLVEQVAGKTGLTRTDVAVVVDSFLEAVKTALEGGSNIEI
                     RGFGTFKVKVRKARKARNPRTGDEVPVPDRKVPVFKPSNEFKGLIIKQPV"
     gene            complement(2429..2698)
                     /locus_tag="AC16GM_05310"
     CDS             complement(2429..2698)
                     /locus_tag="AC16GM_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR2923134.1"
                     /translation="MPIYEYRCQSCSTRFEIFFRSAAEPAACPQCRGTALTRLFSRFA
                     VVRGEAAGDDFDDVSDSSSAPDFGEDSGADACPPEGCGHCQDNLD"
     gene            complement(2701..3597)
                     /gene="sppA"
                     /locus_tag="AC16GM_05315"
     CDS             complement(2701..3597)
                     /gene="sppA"
                     /locus_tag="AC16GM_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR2923135.1"
                     /translation="MARKREWIIGSIIGASFLVFVLLFFIGISSLLRQPGDSDFSGFG
                     PKVAVIDITGVITTAGDVVRQLKKYRSDASVKAILLRIDSPGGGVAASQEIYSEVKKV
                     RDEDKPVVVSMGSVAASGGYYIACAADSIVANPGTLTGSIGVIFEFPVAEQLLKKIGL
                     KWEVVKSGEMKDVGTFTRPLTEKERRHLQGVIDDTYDQFVKVLVENRKLPRRKVLALA
                     DGSIFTGRQAQKLGLVDQLGDYEDAVKLAGRMGGIAGEPRTVKERKRRPSWLDLLTET
                     KNLLQQFTPAGESGPRLLYQFK"
     gene            complement(3610..5541)
                     /locus_tag="AC16GM_05320"
     CDS             complement(3610..5541)
                     /locus_tag="AC16GM_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR2923136.1"
                     /translation="MKRIVAGFIFALLFSAGACSPVLKSKPPVSPPPSRTAPVASADS
                     AGSGSARRTDAGTPSPKVTGKETGLPAGSEENGPPESGEDQAGIDTTAIEPDLEEQLK
                     DIQCLYADGVSLLRERRWELAQAKFEEALDAIADLEIDDQTEPELYERANTLLKEIGG
                     DLKTALVAQGQLGEESSTASFLERFESIKNFKALREQAHLEPLARPDTVQFDVPIVWN
                     QRVQDALTYLQTVAREPFTRYLERMGKYLDLMRGIFRKKNMPLDLCYLPLIESGYSPH
                     AYSYAHAVGPWQFIASTGRLYGLKSTFWIDPRRDFVKSTEAAADFLGDLYQQYNSWYL
                     ALAAYNGGPGRIDRSMRRQKTDDFWKLDLRRQTENYVPLFLAATIIAKQPKKYGFDVV
                     AEAPLRYDIVTVDRSLELEAVAKAMSVDEEELRGLNPELVRGVTPPNLRGYDLRLPAG
                     STQLFLARYEEMPTAKKIRYVSHRVGRGESLSELAGRYGTSVKSIVRVNKLGKGNRLR
                     VGRRILIPVVSGRHPSYARQGAQRPPLAADGTYRIRRGETLTEIAQRHNVSLAELQQV
                     NGLSDPHTVKAGARLKIPPKSLPERIGDSAAVYVVRAGDTLWEIAKKFGITIQQLVVW
                     NPAVDPDNLPVGTRLEIRK"
     gene            complement(5713..6624)
                     /gene="miaA"
                     /locus_tag="AC16GM_05325"
     CDS             complement(5713..6624)
                     /gene="miaA"
                     /locus_tag="AC16GM_05325"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR2923137.1"
                     /translation="MNSILILTGPTAVGKTQLALEIADKRDGEIISADSRQVYRFLDI
                     GTAKPSAEIRDRVPYYLIDILDPTERYSAARFRADALRLIREIAGRGRWPLVVGGTGL
                     YLRALTQGLFQGPGADEKVRERLHKEALEKGTGFLHERLAKVDPKTAARLHQNDRTRI
                     IRALEVYEVTGRPISEWQEKGQYPETEFSFVQVGLQMERSRLYERIDRRVDQMLANGL
                     RREVEKILASGVSPESPGLKTVGYQEVIVHLAGRCSWDKTVALIKQHSRNFAKRQLTW
                     FRSQTDIIWLDAEAPDAARRVLACVPK"
     gene            complement(6667..8514)
                     /gene="mutL"
                     /locus_tag="AC16GM_05330"
     CDS             complement(6667..8514)
                     /gene="mutL"
                     /locus_tag="AC16GM_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR2923138.1"
                     /translation="MQIASSSQRIKILPEELANKIAAGEVVERPASVVKELVENSLDA
                     GATQITIDLKQSGFAEIRVTDDGSGMNREDALLALQRHATSKITSLDDLTRITTLGFR
                     GEALPSIAAVSLLELTTCAGEGTGGTRLVCEAGKVTKVSDIGAPQGTTVTVRELFFNT
                     PARRKFQKSPMAEIRQISEVVTQIALGNSGVAFALTNDGRELLKVPGKQTTVDRLGRL
                     FGKGWAAQLVPLSENLEGVRLDGFAGKPDFSKSNRAQLYLFVNRRPIQSRLLYHAVAA
                     GYGELLPRGRFPVAVVFLTIDPELVDVNVHPTKNEVRFASDRLLHSLLYSAVSRVMQA
                     GGGEKQFATPAVREQSVIPQPLPELVLTGTLSLPVQPDERKSALAAEKAAPLPLPDSR
                     QRLESPAPPSPATLESSEKVSAPLESSQVTADHKPESGTFYQFANVYIISQVKGSLVV
                     VDQHTAHERVLYEEALRMFAASGAHSQQLLFPISLELSPVEYATFTENQDVLAKLGFE
                     LQPFGDRTVLIAGMPMLVREKNPEKFLREVLDDLAENLKAGGDRLKAVAQSYACKAAV
                     KAGDKLTPEEMSALFDQLFATRNPYVCPHGRPTVVRIPVEELNKKFGRS"
     gene            complement(8635..9057)
                     /locus_tag="AC16GM_05335"
     CDS             complement(8635..9057)
                     /locus_tag="AC16GM_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR2923139.1"
                     /translation="MPEPKIRVLLAKPGLDGHDRGVKVIAAAMRDAGFEVIYAGLRQT
                     PEMVVEAAIQEDVDAIGLSILSGAHMTIFPAVLKLLKERGAGDVILFGGGIIPDEEMK
                     QLEALGVGKLFTPGAPTSEIIEYVRKAVKGRKKPAAIL"
     gene            complement(9269..9745)
                     /locus_tag="AC16GM_05340"
     CDS             complement(9269..9745)
                     /locus_tag="AC16GM_05340"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR2923140.1"
                     /translation="MARLPHIFLDGHCYFLTTVTSRRRPLFNDETNCLRLLEDIAFYC
                     KHFDVELHGWVIMPDHVHLLATFARREIISQFVHGVKSHFAHRLRQGSTWQKGCYDHG
                     IRNEKDFFEKLNYIHNNPVTAGLVSDPGEYRFSSYRTYGYDEEGLLPIDKLLLLKA"
     gene            complement(9869..11608)
                     /locus_tag="AC16GM_05345"
     CDS             complement(9869..11608)
                     /locus_tag="AC16GM_05345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR2923141.1"
                     /translation="MVEALAPRTAEPRAVQLGLFSEETIAQVREGKQRWQEKVSKPLT
                     PEQRKQTLPKFITVSGEEIAPLYSPEEIQQINFLRDVGFPGEYPYTRGIHADMYRRRL
                     WTMRQFSGFGTPKQTNERYKFLLAQGQTGLSVAFDLPTLMGYDSDHPRSLGEVGVCGV
                     AVDSLKDMEIIFDGIDLGNISTSMTINSPASILLANYIVVAEKKGVPSHRLRGTLQND
                     ILKEYIAQKEYIYPPEPSLRLITDLMQFCATHVPQWNTISISGYHIREAGSTAIQELA
                     FTLADGFAYVEAALKAGMDVDVFAPRLSFFFNSHLDFFEEIAKYRAARRIWARHMRER
                     YKAKKVESWLLRFHTQTAGVSLTAQQPENNIVRTAWEALAGILGGTQSLHTNSMDETL
                     ALPSEKAAFIALRTQQLLAYESGAANTIDPLAGSYFVEALTNKMEEEAEKYFDEIERR
                     GGVLKCIEEGYFQREIAEAAYRYQKEVEKKERIVVGVNEFVLADEKIEIPILKIPPEV
                     EKGQCRLVQEVRKHRDNDAAQRALANLKRVAEKGENTMPAFIECVRVYATEGEMCDVL
                     RGVFWEYQEPAFV"
     gene            complement(11745..12692)
                     /gene="meaB"
                     /locus_tag="AC16GM_05350"
     CDS             complement(11745..12692)
                     /gene="meaB"
                     /locus_tag="AC16GM_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011013089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR2923142.1"
                     /translation="MNTLERFRQKDPLALSKVISHVENRLSDYQKLLATLYPQTGRAY
                     RIGLTGPPGAGKSTLADKLTLLLVNEKKSVGIIAVDPTSPFTGGALLGDRIRMQDLTE
                     KKEVFIRSMATRGSVGGLAETTRDVALVLDAFGKEFIFIETVGVGQVELDIAEACDTT
                     LVILVPESGDAIQAMKAGLMEIADIFVINKADRPGAARIISELEMILDTRRKMEGWQY
                     PIVATEAINSKGIELLWQKISEHRQVISANGALEAKRRHQVKVQIEKLMASKVQEEVE
                     GKILHGVNLDEVAGRVFRHEVDPYSVCDELWRKRSRLSQ"
     gene            complement(12802..14556)
                     /gene="recJ"
                     /locus_tag="AC16GM_05355"
     CDS             complement(12802..14556)
                     /gene="recJ"
                     /locus_tag="AC16GM_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR2923143.1"
                     /translation="MRRTLARENRAALQWVVAPDPADSGAPTSALARELGLPPALVQI
                     LYSRGYTSAEAIGRFLLPRFSDLCDPFLLPNMQSAIERIVLALAENEKIMVFGDYDVD
                     GLTASALLFLVFNRLGANVSNYVPNRLSEGYGLSPDGFLEAKRRGVTLVVSVDCGITA
                     VDEVAFAREQGIDCVITDHHEPGRVLPKAVAAVNPKLGGEGALGEELSGVGLAFKVAQ
                     ALYRRLGQDELELEEHLDLVALGTSADIVPLIGENRILTKFGLAQIAKTTKPGLKSLA
                     FVAGLMGQEISTGQVVFVLAPRINAVGRLGDAEKAFKLLTTRDELVAAECARHLDAEN
                     RRRKDIDEKTLEEALELIEQQVDFANDRAIILASPGWHLGVIGIVASRLVERFHRPTV
                     LIAIDGAEGKGSARSIPGFHLHDALKECQELLTRFGGHKYAAGLSLPSENIESFRGKL
                     QRVSAERLSEEDLIPKLYLDAEVDFDQIDAKFVELVEQFAPFGPQNMRPTFVTRKAEL
                     FGQPQVVGNGHLKFKARQSASGGDRVFDCIGFGMGHRASELVAGRTKVDLAYVVEFNE
                     WNGARRMQLRLKDLRLSG"
     gene            complement(14672..>14884)
                     /gene="rnpB"
                     /locus_tag="AC16GM_05360"
     ncRNA           complement(14672..>14884)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16GM_05360"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         2898 a         4428 c         4293 g         3265 t
ORIGIN      
        1 acccactggt cgaagaacca acccaaagag cggccggtgg cctcctccat taccttctgg
       61 aattcccgtg tggtgactgt ctgataggcg taggttttgg cgtagttatt tattcccttc
      121 cagaagaggt tgtcgcccag catcccccgc agcatatgca agacccagcc gcctttggga
      181 tagaggtggg aatcaaacag gtcatcggag tcggtgtacc agttatcaac aatcgggcgt
      241 ctgtactcat tttcgtcctc actgatatag gcccgggcgt ggttcagcat ttcgtactgg
      301 aattcgtcct tgccccgatg atgctcagtg tagagcgagg cgaaataggt cgcaaagccc
      361 tcattgagcc aggcgtgggc ccattcgttg caggtgacca gatcgcccca ccactggtgg
      421 gccagctcgt gcgccaccag tccctcgctg gaaacgaccg gttcagaaac ctcggcgtgc
      481 aaagtccggt cggtctgggt ggtggcggag atgttctcca tcccgccata aatgaaatcg
      541 gtcacggcgg actgggcgta ctttgcatac gggtagcgaa atccgatttt ttcggagaag
      601 aatttcacgg cgtccggagt gatgccgaag gagcggcggg ctttcgcttc ccccgtgccg
      661 agcgggacgt agtactccac gggtgtgccg tcgaaatcat cggcatactt ctcaaattct
      721 cccgcgacga cggagatgag ataggtaacg tgcggctgct cctgtgagta atggaaggtc
      781 ttcgtctttt gcgccgggtt gggtttgacc tcgaccagac ggccgtttga aacgaccgtc
      841 caggtttcgg gcacggtggc gaccatttcg gaggtggcgc ggtcgttagg aaaatcccag
      901 caggggaacc agaagtggtt gtcttcatct tccccctgac tccagatctg tttgggcttg
      961 gtgggatacc ccgaatcggg accgacgaag tagagcccct tacgcggctt ggcccagtag
     1021 gcaacgctga attcaatcgg ttttccggcg tcatacgctt ccgggagttt cacgacgaga
     1081 aacgaatcat ccgccgcata gaccagagat ttttgcgccg catcggtgac gccggtaata
     1141 ttgaggtcgc cctggttcag ccggacttcc cggaaaccgg agttgagggg taccaaacgg
     1201 tacgttacct tcccgctgac ttttttctgg tcccagtcga aggtgagatc gagccggatg
     1261 tgctggaggt cgtacgagcg gtcgggcggc cagtggcggg tggagagttc gtcgccgaac
     1321 ggcttataac ggtcggctga agcggggaaa ggggcggtca taatgaagag cactgcccaa
     1381 ctcaggaact ttcttgcgag ttgtattcgc ttcatggaat ctccaccagt tctatgtgca
     1441 gttccggatc ggtctgcagc cggccgtagg tcaatgtgcg gcggtcaaag ttaattgaga
     1501 gactgccggg attaaagagc aaagtcgctc cgaacttctt ggaaaaaggg gtgtgcgagt
     1561 ggccgaagac gatgatgtcc accggctcgg tgaattcggc ccggacacgg tcgaccaatc
     1621 cctcgggggc accgtggccg tggtacaatc cgattttgac cttccccagc gtgacgaccg
     1681 ttttgcgggg caattctttt cgcacctcag gaccgtccat attgccccac acggcgtgga
     1741 ctttggcccg gcggcgcaac tcctccagca ccaccggcgt ggtgaaatca ccggcgtgga
     1801 gcacgatatc ctccggagta aggcgttcga gaaatttctt cgggatcgcc ttgacccggt
     1861 cggggatgtg cgtatcggaa atgatgtaaa cagccatcca gcccctcttt ttccagagaa
     1921 aaagaggaat gcgaacctac tactgtacgc gacgggctct accgcagttt tttcttatgg
     1981 cggttcttgc ggcggcgttt cttccgcttg tgggtgcgga tcttctgtaa cttgcgtttc
     2041 cttccgctgg gcacaagcac ctcctcgtga ttacaccggc tgcttgatga tcaaaccctt
     2101 aaattcattg gaaggtttga aaaccggcac tttccggtcg ggcaccggca cctcgtcgcc
     2161 ggtgcgcggg tttctggctt tgcgcgcctt gcgcactttc accttaaagg tgccaaagcc
     2221 gcgaatctcg atgttgctgc ccccctccag ggcggttttc accgcttcca aaaacgaatc
     2281 gacgaccacg gccacgtcgg tccgggtcag tcccgtcttg ccggctacct gctcaaccaa
     2341 atcggctttg gtcatgctcc ctcctggaat cctggcttag atcgcttacc cacaaaatcg
     2401 gtcacagtgg ccgctgattt tttcacgttc agtcgagatt gtcttggcaa tgcccgcagc
     2461 cttccggcgg gcaggcgtcc gctccgctat cttctccgaa gtcaggcgca ctgcttgaat
     2521 ccgaaacgtc atcgaagtcg tcgccggcgg cttcaccgcg caccacggcg aagcgagaga
     2581 ataaacgggt taaagccgtc ccccggcact ggggacaagc cgccggttcc gcggcgctac
     2641 gaaagaagat ctcaaagcgg gtgctgcaac tctgacagcg gtattcgtaa atcggcatcg
     2701 ctacttgaac tggtagagga gccggggacc gctctcccct gcgggggtga actgttgcaa
     2761 caggttcttg gtctccgtca acaggtcgag ccaggagggc cggcgcttgc gctccttcac
     2821 ggttctcggt tcgccggcaa taccgcccat ccggccggct aatttcacgg catcctcgta
     2881 atcccccagt tggtcgacca aacccagctt ctgggcctga cggccggtga agatcgaacc
     2941 gtcggccaag gccaggactt tgcgccgggg gagctttcgg ttttccacta agaccttcac
     3001 gaactggtcg taggtgtcat caatcacgcc ctgcaggtgg cggcgctctt tttcggtaag
     3061 cggacgggtg aaggtaccca cgtccttcat ttcacccgac tttacgacct cccacttcaa
     3121 gccgattttc ttcaacagtt gctcggcgac cggaaattcg aagatgaccc cgatggagcc
     3181 ggtgagggta ccgggattgg cgacgatgga atcggcggcg caggcgatgt agtagccgcc
     3241 ggaggcggcc accgagccca tggagactac taccggcttg tcttcgtccc gcaccttctt
     3301 gacttcgctg tagatttctt gcgaggccgc cacgcccccg ccgggactgt cgatgcgcag
     3361 gaggatggct ttcaccgagg catccgaccg gtactttttc agttgccgga cgacgtcgcc
     3421 cgccgtagtg atcacgcccg tgatatcaat gacagcgacc ttggggccga agcccgagaa
     3481 gtcggaatct ccgggctgcc gtaggaggga tgagatcccg atgaagaaca ggagcacaaa
     3541 aacgagaaag ctcgctccga tgatgctccc gatgatccac tctcttttcc gcgccattct
     3601 cagcgattcc tattttctga tctcaagccg ggttcccacc ggcaggttgt ccgggtccac
     3661 cgccgggttc cagacgacca gctgctggat cgtgatgccg aattttttcg cgatctccca
     3721 gagcgtatca cccgcccgga cgacgtaaac cgccgccgaa tcgccgatcc gttccggcag
     3781 gctcttcggc gggattttta gccgcgctcc cgctttgacg gtgtgcgggt cgctcaggcc
     3841 attgacctgc tgcaactcag ccagggaaac attatgtctt tgggcgattt ccgtcaaggt
     3901 ctcgccgcgc cggatccggt aggtgccgtc ggcggccagc gggggccgct gggcaccctg
     3961 gcgggcgtaa ctcgggtgcc gacccgaaac gaccggaatc aaaatccgcc ggccgacgcg
     4021 cagccggttc cctttgccca gcttgttcac ccgcacgatc gacttcacgc tcgtcccgta
     4081 ccgcccggcc agctcggaga gcgattcgcc ccggcctacc cggtgggaaa cgtagcgaat
     4141 ttttttggcg gtgggcatct cctcataccg cgccaggaag agttgagtcg acccggccgg
     4201 caggcgcagg tcgtacccgc gcagattggg cggcgtgacg ccccggacca gttccgggtt
     4261 gagtccccgc agttcctctt catccacgct catcgctttg gccaccgctt ccagttccag
     4321 gctgcggtcc acggtgacga tgtcgtagcg cagtggcgct tccgctacga cgtcaaaccc
     4381 gtactttttc ggctgcttgg cgatgatggt ggcggccagg aacaggggca cgtagttttc
     4441 ggtctgccgg cgtaaatcaa gtttccagaa atcgtcggtc ttctggcgcc gcatgctccg
     4501 gtcaatccgg cccggcccgc cgttgtaagc cgccagcgcc agataccagg aattgtactg
     4561 ctggtagagg tcaccgagga agtcggcggc ggcttcggta gacttcacga aatcgcgccg
     4621 gggatcgatc cagaaggtcg acttgagccc gtagagccgt ccggtggagg cgataaactg
     4681 ccaggggccc accgcatgcg catacgagta cgcatgggga gagtagccgc tctcaattaa
     4741 gggcaggtag cagaggtcta aaggcatgtt cttcttcctg aaaatgcccc gcatcaaatc
     4801 caggtacttt cccatccgtt ccaggtagcg ggtgaatggc tcccgggcca cagtctgcaa
     4861 ataggtgagc gcgtcttgca ccctttggtt ccagacgatc ggcacgtcga actggacggt
     4921 gtcgggtctg gccagaggtt ccaggtgggc ttgttcgcgc agggctttga agttcttgat
     4981 gctctcgaac cgttccagaa acgaggcggt ggaactctct tctcccagtt gtccctgagc
     5041 caccagggcc gttttgaggt cgccgccgat ttccttcaac agggtgttgg cgcgctcgta
     5101 gagttccggt tcggtctggt cgtcaatttc cagatcggca atggcgtcta aggcttcttc
     5161 aaacttcgcc tgggccagtt cccagcgccg ctcgcgcaga agcgagacgc cgtctgcgta
     5221 caggcactgg atgtctttca gctgttcctc taaatcgggc tctatagcgg tggtatcaat
     5281 gccggcctgg tcctcaccgg attcaggcgg gccgttctct tctgagccgg ccggaagccc
     5341 cgtctccttc ccggtcactt tgggcgaggg agttccggca tcggtgcgcc tggccgagcc
     5401 gctgccggcg ctgtccgcag aggccacggg tgccgtgcgg gatggcggcg gggaaaccgg
     5461 gggtttcgac ttcaagaccg gggagcaggc acccgccgaa aacagcagtg caaagataaa
     5521 gccggccacg attcttttca tcgtctcatc ctttgcgatg gtcctgcgcc ctccttccga
     5581 agagcacaaa actccaactg cacccgttcg cccgtcccgg gcacagcgcc tctcttcata
     5641 caaggtcgtc gagtttctgt caagtctttt ttgcgcataa ccgtatgtgc gcaaaggaat
     5701 tctcctcctc tttcatttcg gcacacaggc cagcacacgc cgggccgcat ccggggcttc
     5761 agcatccagc cagataatgt cggtctggct gcggaaccag gtcaactgcc gtttggcaaa
     5821 gttccgactg tgctgtttga tcaatgcgac cgtcttatcc cacgaacatc gacctgccag
     5881 atgaacgatc acttcctggt agccgaccgt tttcaatccc ggcgactccg gcgatacgcc
     5941 cgatgccaat atcttttcca cttcccttct taatccgttg gcaagcatct gatcgacccg
     6001 gcgatcaata cgttcgtaca acctgctccg ttccatttgc agcccgacct gcacaaagga
     6061 gaattcagtt tccggatatt gtcccttttc ctgccactcc gaaatcggcc gtccggtcac
     6121 ctcgtacact tccagcgccc ggataatccg cgtccggtca ttctggtgga gccgggcggc
     6181 agtcttgggg tcaacttttg ctaagcgctc gtgcaagaaa ccggttcctt tctccagtgc
     6241 ttccttgtgc aacctttccc tcactttttc atcggctccc gggccctgga acaatccctg
     6301 ggtgagcgcc ctaaggtaaa ggccggttcc acccactacc agtggccacc ggccgcgtcc
     6361 tgcgatttcc cgtatcaacc ggagcgcgtc ggcacggaag cgcgcagccg agtagcgctc
     6421 ggtcgggtca aggatgtcta ttaagtaata cgggacacgg tcccttattt cggcactcgg
     6481 tttggccgtc ccgatgtcca aaaaacgata gacctgccgg gaatcggccg agatgatttc
     6541 accatctctt ttatcggcaa tttcaagcgc cagttgagtt ttgcccaccg cggtggggcc
     6601 ggtgagaatg aggatgctgt tcaagttttg agcagaatta ctggtaagca atgatggaca
     6661 aaaggatcac gatcgaccga attttttatt caactcctcc accggaatcc gcacaaccgt
     6721 cggcctcccg tgggggcaga cgtaggggtt ccgggtggcg aagagctggt cgaagagcgc
     6781 gctcatctcc tccggggtca acttatcccc ggctttgacc gccgccttgc aggcgtagga
     6841 ttgcgccacc gctttcagcc ggtcgcctcc cgccttgaga ttctccgcca gatcgtctag
     6901 cacttcgcgc aggaactttt ccggattttt ctcgcgcacc agcatcggca ttccggcaat
     6961 caagaccgta cggtcaccga acggctgcag ttcaaaaccc agtttcgcga ggacgtcttg
     7021 attctccgta aacgttgcgt actcaaccgg cgaaagttcc agtgaaatcg gaaagaggag
     7081 ttgctgggag tgggcgccag aggcggcgaa catccggagc gcctcctcgt agagtacccg
     7141 ctcgtgggcg gtgtgctgat cgacgacgac cagactgccc ttcacctgcg agatgatgta
     7201 aacgttggca aactggtaga aggttccgct ctcaggcttg tggtcagcgg tgacctgtga
     7261 agattccaac ggcgcgctta ccttttcact cgactcaagc gtcgccgggc tgggaggcgc
     7321 cggactctct aacctttgcc tggagtcggg gagcggaagc ggcgcagctt tttctgctgc
     7381 aagtgctgat tttcgttcgt caggctgtac ggggagcgaa agtgttccgg tgagcaccag
     7441 ttccggcagg ggttgaggga taacgctctg ttcccgcacc gcgggagtcg cgaactgttt
     7501 ctctcccccg cccgcttgca tcacccggct caccgccgaa tagagaagac tgtggagcag
     7561 ccggtcgctg gcgaagcgga cttcgttttt ggtcgggtgg acgttcacgt ctaccagttc
     7621 cggatcgatg gtgagaaaaa ccaccgctac cggaaagcgg ccccgaggca aaagttcgcc
     7681 gtacccggct gcaaccgcgt ggtatagcaa gcgactctga attgggcggc ggttgacgaa
     7741 gaggtacaat tgggcgcggt tcgatttgga gaaatcgggt ttcccggcaa aaccgtccag
     7801 gcgtactcct tcgagatttt ccgacaaagg caccaactga gccgcccacc ctttgccgaa
     7861 caatctcccc aggcgatcaa cagtggtttg cttgccggga accttgagca attcacggcc
     7921 gtcattggtg agggcaaagg ccactcccga atttcccagc gctatctgcg tcaccacttc
     7981 agagatttgc cggatttcgg ccatcgggct cttctggaat ttcctgcggg ccggggtatt
     8041 gaaaaagagt tcgcgcaccg tgacggtcgt gccctgtggc gctccaatat cagatacttt
     8101 tgtgactttg cccgcttcgc acaccagccg agttccgcct gttccttcac cggcgcaggt
     8161 ggtcaattct aacagggaca cagcggcgat cgacggcaag gcctcgcccc gaaagccgag
     8221 cgtggtgatg cgggtcaggt catccagcga agtgatcttg ctcgtggcat ggcgctggag
     8281 ggccagaagg gcatcttccc tattcatccc ggagccgtca tccgtcacgc ggatttcggc
     8341 gaagccggat tgcttgaggt caatggtaat ttgggtggct ccggcgtcga gggaattttc
     8401 caccaattcc ttgaccaccg aagccgggcg ctccaccacc tcgcccgcgg cgattttatt
     8461 ggccagctct tcggggagga ttttaattcg ctgcgacgaa gatgcaatct gcatgtctgt
     8521 gatggaatga gcttgtctca ttaacaccaa atttcgggcg accgggggcg gtcgccctac
     8581 gcgaggcgtt tttctttttc ccgcgtaggc gcggcgccct cgccgcgctc atcgctaaag
     8641 aatcgccgct ggcttcttcc gccctttaac cgctttccgg acgtactcaa taatttccga
     8701 ggtcggcgcg ccgggggtga acaacttccc tacgcccagg gcttcgagtt gtttcatctc
     8761 ttcgtccggt atgatgccgc cgccaaagag gatgacgtca ccggccccac gttctttgag
     8821 cagtttcagt accgccggga agatcgtcat atgcgcgccg gaaagaatcg agagcccgat
     8881 ggcgtccaca tcttcctgaa tggcggcctc gaccaccatt tccggagtct gccgcagtcc
     8941 ggcgtagatt acctcaaagc cggcgtcgcg catcgctgca gcaatcacct tgacgccgcg
     9001 gtcgtggccg tcgaggccgg gtttggctaa aaggacgcga attttcggtt caggcatatc
     9061 atcttttccc tctctaccta cttgcggaat tttcgccttc gcctgagcga tcaaaccgaa
     9121 aaataggtgg aagtaaaata tgtgaaagga ctctgtttgc aaacccctgc tggtacgggc
     9181 gaccgggggg ggaggtcgcc ctacgcggcg gactgtctcg cttcgccacg atggcttttt
     9241 tccgcgtagg cgcggtgccc tcgccgcgct atgccttcag aagcaacaac ttatcgatag
     9301 gtaacaagcc ctcttcatca tacccatatg tccgatagct tgaaaatcga tactcgccgg
     9361 ggtcactgac cagaccggcg gtcaccggat tgttgtggat gtagttcagt ttctcaaaga
     9421 aatccttctc gttccgaatg ccgtgatcat aacacccctt ctgccaggtc gaaccttgtc
     9481 gcaaacggtg agcaaagtgg cttttaaccc cgtggacaaa ttgcgaaata atctctcttc
     9541 ttgcaaaggt tgccagaaga tgcacgtggt cgggcataat cacccagccg tgtaattcta
     9601 cgtcgaagtg cttacaatag aatgcgatgt cctctaacag gcgcaaacaa ttggtttcat
     9661 cgttgaatag aggacggcga cgggaagtta ccgtggtgag aaagtaacag tggccatcaa
     9721 gaaagatatg cggaagtcga gccatcacat gtaacaggct gggcgaccga gggcggtcgc
     9781 cctacgcggg gagattccct ccctctcgcg tgggcgcggc gcccacgccg cgccgattta
     9841 actgagtgaa caagttatgc ataaggaact acacgaacgc cggctcctgg tactcccaga
     9901 agaccccgcg caggacgtcg cacatttccc cttcggtggc gtagacccgc acgcactcga
     9961 tgaaggcggg catcgtgttc tcgcctttct ccgccacgcg cttgaggttg gccagcgcgc
    10021 gctgggccgc gtcgttgtcg cggtgcttgc ggacctcctg caccaaacgg cactgtccct
    10081 tttccacctc agggggaatt ttcagaatcg ggatttcgat tttctcgtct gcgagaacaa
    10141 attcattgac gccgacaacg atccgctctt tcttttccac ttccttctgg tagcgatagg
    10201 ccgcttcggc aatctcgcgc tggaagtacc cttcctcgat gcacttcaac accccgcccc
    10261 ggcgctcgat ttcgtcaaag tacttctccg cctcttcttc cattttgttg gtcagtgcct
    10321 caacgaagta ggaaccggcc agcggatcga tagtatttgc cgcgcctgat tcgtaagcta
    10381 acagttgctg cgtcctcagc gcaatgaaag ccgctttttc cgaagggagg gccaacgttt
    10441 cgtccattga attcgtgtgc aaagactgcg tgccacccag aatgcccgcc agtgcttccc
    10501 acgcagtgcg gacgatgttg ttctccggct gttgggccgt taaagacact ccggcggtct
    10561 gcgtgtggaa acgcaaaagc cacgactcca ccttcttggc tttgtaccgc tcccgcatgt
    10621 gccgggccca gatccgccgc gccgcacgat acttggcaat ttcttcaaag aaatcgaggt
    10681 gagagttgaa aaagaacgaa aggcggggtg cgaagacatc cacgtccatc ccggctttga
    10741 gtgccgcttc gacgtaagca aagccgtcgg ccagcgtgaa agctaattcc tgaatcgcgg
    10801 tcgaccccgc ttcccggatg tggtagccgg agatggaaat cgtattccac tgcggcacgt
    10861 gggtggcgca gaactgcatc aggtcggtga tgagccgcag ggagggctcc ggcgggtaga
    10921 tatactcctt ctgggcgatg tattctttta atatgtcatt ctggagcgtg ccccgcaggc
    10981 ggtgcgaggg cactcccttc ttctcggcca cgacgatgta gtttgccaga agaatcgaag
    11041 ccggtgagtt gatcgtcatt gaggtggaga tattccccag gtcgatgccg tcaaaaatga
    11101 tttccatatc tttgagcgag tcgaccgcca cgccgcagac gcccacttct cccagcgagc
    11161 ggggatggtc ggagtcgtat cccatcaggg tgggcagatc gaaagcgacc gaaagacccg
    11221 tctggccctg ggcgaggagg aacttgtagc gctcgttagt ctgcttgggc gtgccgaagc
    11281 cggagaactg gcgcatcgtc cagagcctgc gccgatacat atcggcgtgg atgccccggg
    11341 tgtaggggta ctcgccggga aagccgacgt cgcgcaggaa atttatctgc tggatctctt
    11401 cgggagaata gagcggagcg atttcctcgc cggagacggt gatgaacttc ggcagggttt
    11461 gcttgcgttg ctccggggtc agcggtttgg aaactttttc ttgccagcgc tgcttgccct
    11521 cgcggacctg ggcgattgtc tcttcggaaa aaagccccag ctgaacagcg cggggttcag
    11581 ccgtccgggg tgcaagggct tcgaccattc ggttcatatt tcccttcatt tttttgtcgg
    11641 agactcgact cttcgagact gatggtatcc ttgatttctt tttcaccggg cgggactgca
    11701 ctttctgcat tatgcgcatg gcatcattaa tatagcaacc cgcatcactg cgacaaccgg
    11761 ctccgcttcc gccagagttc gtcgcagacc gagtacggat cgacttcgtg gcgaaaaacc
    11821 cgccccgcca cctcatcgag gttcacgccg tgcaaaatct tcccttcgac ttcctcttga
    11881 actttactcg ccataagctt ttcaatctgc actttaacct ggtggcgcct tttggcctcc
    11941 agcgcaccgt tcgcggaaat cacctgccgg tgctcgctaa ttttctgcca caagagttcg
    12001 atgcctttac tattgatcgc ttccgtggcc acaatcgggt actgccaccc ctccattttg
    12061 cggcgggtgt ccagaatcat ctccaattca gaaataattc gcgccgcgcc ggggcggtcg
    12121 gccttgttga tgacgaagat gtcggcaatc tccatcaagc ccgccttcat cgcctgaatg
    12181 gcatcgcccg attcggggac taaaatcacg agcgtcgtat cgcaggcttc cgcgatgtcg
    12241 agttccacct ggcccacgcc aaccgtttct atgaaaatga actccttgcc gaaggcgtcc
    12301 aaaaccaagg ccacgtcgcg agtggtttcg gccaggcccc ccaccgagcc gcgggtggcc
    12361 atgctgcgga taaagacctc ttttttttcg gtcaaatcct gcatccggat ccggtcgccc
    12421 aagagcgcgc cgccggtgaa aggcgaggtg ggatcgacgg caatgatccc caccgatttt
    12481 ttttcgttga ccagaaggag ggtgagtttg tcagccagcg tgctcttccc ggccccgggc
    12541 gggccggtga ggccgatccg gtaggcccgg ccggtttggg gatagagtgt cgccagcaat
    12601 ttttggtagt ccgacaggcg gttttcgaca tgggaaatga ctttggaaag ggccagcgga
    12661 tctttctggc ggaagcgttc gagagtgttc acgttttccg ataattgggg gcgaccgagg
    12721 gcggtcgccc cacgcggttg gggtaacagc agggattcgg tgagcgaacc cctacgacag
    12781 acaatccgac acggcagacc atcacccgga caggcgcagg tctttcagcc gcagttgcat
    12841 ccgccgggct ccgttccact cgttgaattc caccacgtag gccaagtcca ctttcgttcg
    12901 accggcgaca agctcactgg cccggtggcc cattccgaaa ccgatacagt cgaagacgcg
    12961 gtcgccgccc gaggcggact gccgggcttt gaatttcaga tgcccattcc ccaccacctg
    13021 gggctggccg aagagttcgg ctttgcgggt gacgaaggtc ggccgcatat tctgagggcc
    13081 gaacggcgca aactgctcca ccagttcgac gaatttggca tcgatctggt caaagtcaac
    13141 ttcggcatcc aggtacagtt tcggtatcaa atcctcttcc gaaagccgtt cagcggaaac
    13201 gcgctgcagt tttccccgga aagattcaat gttctccgac ggcaaggata aaccggcggc
    13261 gtacttgtgt ccaccgaagc gggtcaaaag ttcctgacac tccttgagag cgtcatgcag
    13321 gtggaaaccg gggatgctcc gggccgagcc cttcccttcg gcgccgtcga tggcgatcaa
    13381 gaccgtgggc cggtgaaaac gctccaccag gcgcgaggcc acgatgccga tgacccccaa
    13441 atgccagccg ggggaagcca ggatgatggc ccggtcgttg gcaaaatcaa cctgctgttc
    13501 gatcaattcc agcgcctctt ccagggtctt ctcatcgatg tccttgcgcc gacggttttc
    13561 ggcatcgagg tggcgggcac attcggcggc caccagttca tcccgggtgg tcaaaagttt
    13621 gaaggccttc tcggcatcgc ccagacggcc gacggcatta atgcgcggag ctaagacaaa
    13681 aaccacctga cccgtggaaa tttcctggcc catcaagccg gccacaaaag ccagagactt
    13741 caagccgggt ttggtcgtct tggcgatttg ggccaggccg aatttcgtca gaatccggtt
    13801 ctccccgatc aggggcacga tgtccgccga cgtgcccagg gcaaccagat cgagatgttc
    13861 ttccaactct aactcatcct gtcccagccg gcggtagagc gcctgagcta ctttgaaggc
    13921 caaacccact cccgaaagtt cctcgccgag cgccccttct ccaccgagtt tgggattgac
    13981 cgctgctacc gccttcggca gtacccgtcc gggttcgtgg tggtcggtaa tcacgcagtc
    14041 aatcccctgc tccctggcga aggccacttc gtcgaccgca gtgatgccgc agtccaccga
    14101 aacgaccaag gtcacgccgc ggcgcttcgc ttccaggaag ccgtcgggcg agaggccgta
    14161 gccttcggaa agacggttgg ggacgtagtt ggagacgttg gctccgagcc ggttaaacac
    14221 caaaaagagt aaggccgaag cggtcaagcc atccacgtcg tagtcgccga aaaccataat
    14281 cttttcgttt tcggcgagcg ccaaaacgat ccgctcgatg gccgactgca tattgggcaa
    14341 aaggaagggg tcgcagaggt cggagaagcg gggcaaaaga aatctcccaa tcgcctccgc
    14401 cgaggtgtag ccccggctgt agaggatttg caccagcgct ggcggcaagc ccaattcacg
    14461 ggctaaagcg gaggtaggag ccccgctgtc ggcggggtcg ggcgccacca cccactggag
    14521 cgcagctctg ttttcgcggg cgagcgttcg gcgcactggt aatctatgtt ttctcgaagc
    14581 ggagtggacg aactttcatt cagcctttga aactcgtttg tggaagacga aacttgccgc
    14641 cggcccaaaa gaccaagcat tacgcttttt cacccacaac cggccctaag ccgggttctg
    14701 tcgatccccc gccgaggcgg gggacgggca gccatttctc tgggccctgc attgctgcgg
    14761 ggctcaagcg acctacccgc ggatctagcg caaggagcct ttgctttccg cttatctggt
    14821 cttgctccca gtggggtttg ccgtgccccc gatgttacca tcggggcggt ggtctcttac
    14881 accg
//
LOCUS       DBNGZD010000201         4150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1068710, whole genome shotgun sequence.
ACCESSION   DBNGZD010000201 DBNGZD010000000
VERSION     DBNGZD010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1068710"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="AC16GM_05365"
     CDS             <1..301
                     /locus_tag="AC16GM_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR2923144.1"
                     /translation="VNLELVKAGLARVYTFPPNVRYAQRFMAAQKEARSRKAGMWGIP
                     VWSLEAYYVGNSRSLKFHRPQCRQARRLKSSEKVKFSSRDAALDSGYAPCRNCKP"
     gene            638..874
                     /locus_tag="AC16GM_05370"
     CDS             638..874
                     /locus_tag="AC16GM_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923145.1"
                     /translation="MLNRRGAEGFFEGTESLDVTEEAAGEVGTMVGRDGLDAERIEAY
                     QSASGGKATAVWGVSSGYRETNCKRVNPPTAENW"
     gene            1502..1714
                     /locus_tag="AC16GM_05375"
     CDS             1502..1714
                     /locus_tag="AC16GM_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923146.1"
                     /translation="MKVEKTRGEFYCATVEEAENKKFRRALRYRGLEKKWKKNLALTK
                     KIVKIFIAGFARERLEIAPSAMFVYF"
     gene            1772..2902
                     /gene="asnA"
                     /locus_tag="AC16GM_05380"
     CDS             1772..2902
                     /gene="asnA"
                     /locus_tag="AC16GM_05380"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR2923147.1"
                     /translation="MTDKRADLAGPGIGSYDELEKILPQDYSSLLNPKDTQKGIFAVK
                     NYIEENLCRELNLMMVQVPLIVDIESGVNDLLDRDGSRTPIQFHISNDYDKHPIDAQV
                     VQAATKWKRVALKQLQMQVGEGLCTDMRAVRKDYFLDHDHSVYVDQWDWEQVITERQR
                     NLEYLKEVVRKIWKVLKGAERHAQKLFPRLKTDRYPDLPEELTFLHAEDILDRFPDLP
                     RKKRETAILQEYPAIFIIGVGWTLKDGYPHEMRAADYDDWVTETVSEDGRPMHGLNGD
                     ILVWNPVTRRRHELTSMGIRVNAVTLKKQLEITGLLDFLKFPYHQAIINNEIPLSSGG
                     GIGQSRTYMLLLKKAHLGEVSVTVWPKILKELCRKRNIFVLE"
     gene            3068..3997
                     /locus_tag="AC16GM_05385"
     CDS             3068..3997
                     /locus_tag="AC16GM_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2923148.1"
                     /translation="MAEKQTGKVIQVEHLRKVYGSVIAVDDISFEVYQGEIFGMVGPN
                     GAGKTTTIECVEGLRKPDQGHIRVLELDPQKNGYALRERMGVQLQEAVLPERMKVWEA
                     LDLYAAFYPRHTDWESLLTRLGLAERRSAHFAKLSGGQKQRLFIALALIHDPEVVFLD
                     ELTTGLDPHARRALWDLVREVRDQGKTIFLTTHFMEEAERLCDRVAIVDHGRIVALDS
                     PENLIRRLGVENRVVFSVDGSFNSEQLSHLAEITKVERIGERVIVYGRGDSLVRVVVD
                     SLSANGFRFRDLRTEQPTLEDVFLSLTGRELRE"
BASE COUNT          971 a         1084 c         1247 g          848 t
ORIGIN      
        1 ggtcaatctg gaactggtca aagccggatt ggcccgggtc tatacctttc cgcccaatgt
       61 ccgctacgcc cagcgcttca tggccgcgca aaaagaggcg cgcagccgca aagcagggat
      121 gtgggggatt cccgtctggt cgctggaagc atactacgtc ggtaatagcc gcagtttaaa
      181 atttcaccgt ccgcaatgcc gccaagcgcg tcggctaaag tcgagcgaaa aagtcaagtt
      241 ttcctcccgc gatgcggcgc tggacagcgg atacgcgccg tgccggaatt gcaagccgtg
      301 agcgttctcg ccttagtcaa acgcaggggt gggcgttcca gaattgccac aggagaccaa
      361 gcagcgcacc gtgaaaactg tactaatcac agacataggc aacagcgtag ttgggggaga
      421 gggtagggtt ttgggtgaag gtgcgcaagc gttggatggg ggcgaggtat cccaaggcgc
      481 tgtgcgcccg atgttcgttg taacacttga ggtagttgac cacccctctg ggggagaggt
      541 ggggatgtcc cagagtgcgg tagaattctt cgttgtcggt gcggtgggag tccgccagag
      601 gcggattgta gcgcggggta cccggaggga tgagggtatg ctgaatcgca gaggtgcgga
      661 gggtttcttc gaagggacgg agagtcttga tgtgaccgaa gaagcggcag gtgaggtcgg
      721 caccatggtc ggtcgggatg gtctggatgc ggaaaggata gaagcgtacc agtccgcctc
      781 aggcggaaag gcgaccgcgg tctggggcgt gagctcgggg taccgcgaga caaattgcaa
      841 gcgggtgaac ccaccgacgg cggaaaactg gtaggcgcgc cgaggcattc ccgggcgcag
      901 ggagcggtat ttgacatcca ccgggacttt ctccccgggg aaggtcaatc cgcctcaggc
      961 ggacgacgcg tgtatctctt tctccggggc ttgcgagaat ggagctgtcc ggcccgcttc
     1021 aggatgttgt agatgccgga gattcgcctg aggcgaactg cttcgcgaga agaaccagtg
     1081 caagcgtcgc ggcccatacc ccgtctcgag gcgtaaccgc agtaccttgc gcgtccagta
     1141 cttcccgcat cgtttcggac tgcgcttcgg cttgcgggag tgattgtcca gactctccgg
     1201 ctgaccgcca ctcgcctccc atcggtggcg ctacttgtaa aacgtcttgg cggaaatgcc
     1261 atacctccga catacccgcc gcgcattgcc gcactgcccg tactcacgaa accattgcat
     1321 ccgccggtgc cgatcagtat agtctgtact atcgggcatg ggtaacctcc gtgttctttg
     1381 aacgtcccag tccaattaaa cacgtgttac ccatgtctga aatctaaaca agcaccgtga
     1441 aggtttctag aattgacaga acaagattta tcacctgccc tttgaccaac agtacgataa
     1501 tgtgaaagtt gaaaaaacaa gaggagaatt ttattgtgct acggtggagg aagcagaaaa
     1561 caaaaaattc cgtagggctc ttagatatag aggtttagaa aagaagtgga aaaagaattt
     1621 ggcgctaacg aagaaaatcg ttaaaatttt catagcgggc ttcgcccgcg aaagacttga
     1681 aattgcccct tcggcgatgt ttgtttactt ttaggttgtg ctggcggttc ctcttatgaa
     1741 acgctgaaaa ttgcaaccgg aggtagatag catgacagac aaaagagcag acctggcggg
     1801 gccgggcatc ggcagctatg acgaactgga gaaaatcctg ccgcaggact atagctcgct
     1861 tttgaacccc aaagacaccc aaaaggggat ctttgcggta aagaactaca tcgaggagaa
     1921 tttgtgccgg gaactcaacc tgatgatggt acaggtgccc ctgatcgtgg acattgagag
     1981 cggcgtgaat gacctcctcg accgggacgg gtcccgcacc cccatccagt tccacatttc
     2041 caacgattat gataagcacc ccattgacgc ccaggtggtg caggccgcga ccaaatggaa
     2101 acgggtggcg ctgaaacagc tgcagatgca ggtcggcgag ggcctgtgca ccgacatgcg
     2161 cgcggtgcgc aaagactact tcctcgacca cgatcacagc gtctacgtgg accagtggga
     2221 ttgggagcaa gtgattacgg aaaggcagcg gaacctggag tacctgaagg aagtagtccg
     2281 aaaaatctgg aaggttctca aaggcgccga acggcacgcc caaaaacttt tcccccggct
     2341 gaaaacagac aggtatcccg acctgccgga ggaattgacg ttcctccatg ccgaagatat
     2401 cctggatcgg ttccccgacc tgccgcgcaa aaagcgcgag acggcgatcc tgcaggaata
     2461 ccccgcaatt ttcatcattg gcgtcggctg gacgctcaag gacggctacc cccacgaaat
     2521 gcgggcggct gattacgatg actgggtcac cgaaacagtg tccgaggacg gccggcccat
     2581 gcacggactc aacggagata tcctggtctg gaacccggtc acccggcgcc gccacgaact
     2641 gacctccatg ggtatccgcg tgaacgctgt gacactcaag aagcagctgg agattaccgg
     2701 cctgctcgat tttctcaaat tcccctacca ccaggcaata atcaataacg agattccgct
     2761 cagcagtggt ggcggcatcg ggcagtcccg cacctacatg ctcttgctca agaaggcgca
     2821 cttaggcgag gtcagcgtta ccgtgtggcc gaagatactg aaggagttgt gccggaagag
     2881 aaatatattt gttctggagt gaacggaaat cgggccgata atgctcctct ccgagcgcag
     2941 cttacgcaaa cccttctgtc cgcagtgaaa atcgcaggta aggtacttat atgatttgcc
     3001 ataaatcgta actctgggga agaattaacg taggtattaa tcaggactga cgggtgattc
     3061 tacattgatg gcagagaagc aaacgggaaa agtcattcag gttgaacacc tgcgcaaagt
     3121 ctacggttca gtcattgccg tagatgacat ttcgtttgag gtgtaccagg gggaaatctt
     3181 cgggatggtg gggccgaatg gagccggcaa aaccacgact attgagtgtg tcgaaggcct
     3241 gcggaagccg gaccagggac acattcgtgt cctggaattg gatccccaga aaaacggtta
     3301 tgcactccgc gagcggatgg gggtgcagtt gcaggaggcg gtgctccccg aacgcatgaa
     3361 agtctgggag gcgctggatc tctatgccgc attttacccc cgccataccg actgggagag
     3421 tttgctgacg cggctggggc tggcggaacg gcgcagcgct cactttgcca aactttccgg
     3481 cgggcagaaa cagcgccttt tcattgcgct ggcactcatc catgacccgg aagtggtttt
     3541 cctggacgaa ttgaccaccg gtctcgatcc ccacgcccgc cgggccttgt gggatttggt
     3601 gcgggaagtc cgtgaccagg gaaagacgat ttttctcacc acacacttta tggaagaagc
     3661 cgaacggctc tgtgatcgtg tcgcgatagt agaccacggc cgcattgtcg ccctggactc
     3721 gccggagaac ctgatccgca ggctgggtgt ggagaaccgg gtggtgttca gtgtcgatgg
     3781 aagcttcaat tcggaacaac tcagccattt agccgagata accaaggtcg agagaatcgg
     3841 ggaacgcgtc attgtctacg gacgcggcga tagcctggtc agggtcgtgg tcgattctct
     3901 ctccgcaaac ggtttccgtt ttcgcgacct gcgtaccgag cagccgactc tggaggatgt
     3961 ttttctgtca ctcaccggcc gggaattacg agagtaacgg agcaactatg cgtggtgtct
     4021 ggaaactgac ctggatggaa atgaagctct tcctgcgcca gccggcggcg gcgttcttca
     4081 ctctggcctt tccgcagatg ttgctgctcc tcttcggcag tgtcaacggc aacaaaccga
     4141 cgcccttctt
//
LOCUS       DBNGZD010000202         4397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1698743, whole genome shotgun sequence.
ACCESSION   DBNGZD010000202 DBNGZD010000000
VERSION     DBNGZD010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1698743"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="AC16GM_05390"
     CDS             <1..1298
                     /locus_tag="AC16GM_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR2923149.1"
                     /translation="QKYDEEEEVLFETQKIAEVTAPPAPVEPKKKMPVAVSQPPLREK
                     TPVPAPRSSGKKPLWPWAVAAILLLALTADFSLGLSPLGKKLYKGRTGLSTFIASVPP
                     GATVFIDGKAIDQPTPAVVSEMKTGKHSVILSFPGYADLHTEVDCRTGQANRFNFAFR
                     GTLEIASEPAGAEVFCNGVKEPQRTPVALSVPIDSVLSVELVYGKSRLKPISFSLLTL
                     ANDVGASWQVEKVASDHLRLTGRFTRELVFRSTPPGADLFIDHESKPAGNTWSAPLVS
                     VPFGKHTVTARLAGYAPQHIMLEVSEKTTGEYSFSLADQTARRGTSSSQPFTGSKDGK
                     SLSLANLNSKVVVTVQDEQKKPLSGAQLVALNLDSKERRPLGKTDSQGALACDLPDGR
                     WKVSIFLVSYQTESKEFRVRSGSQEKLQFVLRKLEPERP"
     gene            1366..2322
                     /locus_tag="AC16GM_05395"
     CDS             1366..2322
                     /locus_tag="AC16GM_05395"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR2923150.1"
                     /translation="MKIEIAARTDLGRVRERNEDAFLVLPEQRLAMVCDGMGGHRAGE
                     VASRGAVTTIKELANPTHRERLTGLFFSIDQKLPPAVRQLISSLRVANRRIYNAAQGD
                     VSLRGMGTTVVAALCDSGGCAIGHVGDSRAYLFRGGVIHRLTSDHSWLAELIAAGELT
                     VADEHSFAERNVITRSLGTRPVVKIDLRWDPVEKDDLILLCSDGLSGHLKDPDLLKVI
                     TAKKKKLEAVVEQLIKRANEAGGEDNITCALIRVVETDTTPVERFQGTVQEETDGELT
                     VEDQIIQSLLGGKKLQSEVSFESTDAIRIKAKEKKKKFSLFS"
     gene            2540..2797
                     /locus_tag="AC16GM_05400"
     CDS             2540..2797
                     /locus_tag="AC16GM_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2923151.1"
                     /translation="MVNGKFYVYVLKSQVDERTYTGSAANVTARVRAHNAGRVSASRL
                     HRPYDLVYTEEFQTRAEAMAREKFFKTVAGRIELRLVLQQL"
     gene            2822..2894
                     /locus_tag="AC16GM_05405"
     tRNA            2822..2894
                     /locus_tag="AC16GM_05405"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2855..2857,aa:Ala,seq:cgc)
     gene            3070..3335
                     /gene="ffs"
                     /locus_tag="AC16GM_05410"
     ncRNA           3070..3335
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16GM_05410"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            3410..>4397
                     /gene="dnaX"
                     /locus_tag="AC16GM_05415"
     CDS             3410..>4397
                     /gene="dnaX"
                     /locus_tag="AC16GM_05415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR2923152.1"
                     /translation="MAYVVFARKYRPQTFAEVVAQEHVTQTLVNALSSGKISHAYLFS
                     GPRGTGKTTTARILAKALNCVNGPTPEPCNTCGFCQEITSGQSLDVVEIDAASNSGVD
                     DVRTLRENIRYTPAGGKYRVYIIDEVHMLSGAAFNALLKTLEEPPVHSVFVLATTELH
                     KVPATIVSRCQRFDFRRIPFERLSTALTDIAVKEHLAIDADAVALVAKKAEGSLRDGL
                     SLLDQLVAFAGERITRSEVEATLGLIAGEVLFEITDAIAARDEKQVLSVFQKQADAGA
                     DLKQFLDDLLEHFRNLLIARTAGDKAELFALATAERERYVTVAQTFAEGDLLR"
BASE COUNT          982 a         1234 c         1309 g          872 t
ORIGIN      
        1 agcagaagta cgatgaggaa gaagaagtat tatttgaaac gcagaaaata gccgaagtga
       61 ctgctccgcc ggcgccggtt gaacccaaaa agaaaatgcc tgtcgcggtc tcccagccgc
      121 ctctccggga aaagacgccg gtcccggctc cccgctcttc cggaaaaaaa cccctctggc
      181 cctgggcggt agcggccatc ctgctcttgg cgctgaccgc tgatttttcg ttaggtcttt
      241 cgcctctggg gaagaagttg tacaaaggca gaaccggcct gagcacattc attgcctcgg
      301 taccgccggg agcgacggta ttcattgacg gcaaagcaat cgaccagccg acgcccgccg
      361 tggtgtctga aatgaagacg gggaaacaca gcgtcattct ttccttcccc ggttatgccg
      421 acctccacac ggaggtggat tgccgaacgg ggcaggcgaa tcgcttcaac tttgctttcc
      481 gcggcactct cgagatcgct tccgaaccgg cgggagcgga ggtattctgt aacggtgtca
      541 aggagccgca gcgcacaccc gtcgcgctca gcgtaccgat cgattcggtt ttgtctgtgg
      601 agttagtgta cggcaagtcg cggctcaaac cgatctcctt cagcctcctg acgctggcca
      661 acgacgtcgg cgcgtcgtgg caggtcgaaa aggtcgcctc cgatcatctc cgcttgaccg
      721 gacggttcac ccgtgagctc gttttccgct ccaccccgcc gggggccgat ctgttcatcg
      781 accatgagtc caagccggcg gggaatacct ggtcggcacc tttggtaagc gtgccgttcg
      841 gcaaacacac ggtcacggcg cgcctggcgg gttatgcgcc gcaacatatc atgctcgagg
      901 tttcggagaa aaccacgggc gagtattctt tcagcttggc agaccaaacc gcgaggcggg
      961 ggacgagcag ttcacagccg ttcaccggca gtaaagacgg gaaatcgctt tcgctggcga
     1021 acctgaacag caaagtagtc gtaaccgtcc aggacgaaca gaaaaaacca ttgtccggcg
     1081 cacagctggt ggcgctcaat ctggactcca aagagcgacg ccccctgggg aaaaccgaca
     1141 gccagggcgc gctcgcctgc gatttacccg acggccggtg gaaagtttcg atttttttgg
     1201 tttcttacca gaccgagtcg aaagagtttc gcgtgcgctc cgggtcgcag gagaaactgc
     1261 agttcgttct gagaaaactg gagccggagc ggccatgagc cggtttgata gaccgggtag
     1321 aaaatccttg aaacgaacga aacaatacat tatgataggc cggatatgaa aatcgaaatt
     1381 gcggcccgga ccgatctggg acgggtacgg gaacggaatg aggatgcttt tctggtgttg
     1441 ccggaacagc ggctggccat ggtctgcgac ggcatggggg gacaccgggc gggcgaagtc
     1501 gccagccgcg gggcggtgac caccatcaaa gaactggcca acccgaccca ccgggagcgg
     1561 ctgaccggac ttttcttttc cattgatcag aaactccctc cggcagtgcg tcaattaatc
     1621 tcatccctgc gggtggcgaa ccggcgcatt tataacgccg cccagggtga cgtgtccctg
     1681 cgcgggatgg ggaccacggt ggtggcggcc ctgtgcgatt cgggcggctg cgccatcggc
     1741 cacgtggggg acagccgcgc ctacctcttc cgcggcggcg tcattcaccg gttgacgagc
     1801 gaccactcct ggctggcgga actcatcgcc gcgggcgaac tgaccgtggc ggacgaacac
     1861 tcctttgccg agcggaacgt catcacccgc tctctgggga cgcggccggt ggtcaaaatc
     1921 gacctgcgct gggatccggt ggaaaaagac gacttgatcc ttctgtgcag cgacggcctc
     1981 tccggccatc tgaaggaccc ggacttgttg aaggtcatca ctgccaaaaa gaaaaaatta
     2041 gaggccgtcg tcgaacaact gattaaaaga gcgaacgaag cggggggcga agacaatatc
     2101 acctgcgccc tcatccgggt ggtcgagacc gacaccacgc cggtggagcg cttccaggga
     2161 accgttcagg aagaaaccga cggcgaactg accgtggaag accagatcat ccaatcgctc
     2221 ctggggggca agaagttgca gtcggaagtg tccttcgaat ccaccgacgc catccggata
     2281 aaagcaaaag agaagaaaaa gaaattcagt ttgttctctt aaccgagtca ttccatccgc
     2341 gtcctgattc tcccgtaagc gctcagtatt ttttctcctg ccactcatcc tggaaccagc
     2401 gtgatcggct cgttgatttc ccacttgaga aactgttata ttcggaaaaa cggaaggaac
     2461 ggaatctctc tggggcagta gctcagtccg cctgaggcgg attcgaagcg ctccgctcca
     2521 tagatagtcg agaagcagaa tggtcaatgg aaagttctac gtttatgttt taaagagtca
     2581 ggttgacgaa cggacataca ctggttctgc cgctaatgtt acggctcgtg tccgagcgca
     2641 caacgctgga cgagtctcgg cctcgcggct acaccgaccg tatgatttgg tgtatactga
     2701 ggaattccag actcgtgcag aggccatggc tcgcgaaaaa ttcttcaaaa ccgtagcagg
     2761 acgaatcgag ttaagattgg ttttacagca attatgaggg taaaaaggaa taaggaaatc
     2821 tggggcagta gctcagctgg gagagcatca cgttcgcaac gtgagggtcg gcggttcgaa
     2881 tccgctctgc tccataataa ctttggtgaa ggaggaaatt gaaaggatgg agcgcagcga
     2941 tttcggtcgg tacgactttc ggtcgttccg cccctaacaa catgagggtc ggttgagcgg
     3001 agcgaaagtc gtatcgatcc gcctgaggcg gattcgaatc cgctctgctc catggaaaaa
     3061 aagatgcgct cgtgctagat ggggagttag cggtgccctg taacccggaa tccgctctac
     3121 cgggatcgac gcccaccgga ggtctcacta ccgcggatcg tgcggccagg ggccgacgtt
     3181 gacggtcagg tcctgcgcaa cacggaggcg cccaaccccg ccaggcccgg aagggagcaa
     3241 cggtaagcgt ttttccgggt gtgccgcagg gtcgccgggc ccgagccggt caccggatac
     3301 gcaaactgcg gcatagatcg agggtgggtg cacgagcgtt aaatagattc acagcccttc
     3361 gacgttgctc agggtggtga gtctgccgaa ccacagatta taaactacta tggcctacgt
     3421 cgttttcgcc cgcaagtacc gaccccagac cttcgccgag gtggtggcgc aagagcacgt
     3481 cacccagacc ctggttaatg cgctttcctc gggcaagatt tcgcacgctt acctcttttc
     3541 cgggccgcgc ggcaccggca agactaccac cgcgcggatt ctggccaaag cgctcaactg
     3601 cgtgaacggc ccgactccgg aaccgtgcaa tacctgcggg ttctgccagg aaattacctc
     3661 cggccagagt ctggatgttg tcgaaattga tgcagcctca aattcgggag tggacgacgt
     3721 gcggacgttg cgcgagaata tccgctacac gccggccggc gggaaatacc gggtctacat
     3781 cattgacgaa gtccatatgt tgtccggcgc tgctttcaat gcgctcttga aaacgttaga
     3841 ggagccccct gtgcactcgg tcttcgttct ggcaacaacc gaactgcaca aagtcccggc
     3901 gaccattgtc tcacgctgtc agaggtttga tttccggcgg attccgttcg agcgactctc
     3961 cacagctttg accgacatcg ctgtcaagga gcaccttgca attgacgccg acgcggtggc
     4021 tttagttgcc aagaaagccg aaggaagttt gcgcgacggg ctttcgcttc tggatcagct
     4081 ggtggctttt gccggtgaac ggatcacaag aagcgaagtc gaggccacgc tggggctgat
     4141 tgcaggagag gtccttttcg agattaccga cgccatcgct gccagagatg aaaagcaggt
     4201 gctgtcggtg tttcaaaagc aggccgatgc cggggccgac ctcaaacaat ttctggacga
     4261 tttgctagag catttccgca acctgctcat tgcccgcacg gcaggcgaca aggccgagtt
     4321 attcgcgctg gcaacggccg agcgcgagcg ttacgtcact gtcgctcaaa cttttgccga
     4381 aggcgacctt ttgcgga
//
LOCUS       DBNGZD010000203         4417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1837731, whole genome shotgun sequence.
ACCESSION   DBNGZD010000203 DBNGZD010000000
VERSION     DBNGZD010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1837731"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1106)
                     /locus_tag="AC16GM_05420"
     CDS             complement(174..1106)
                     /locus_tag="AC16GM_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018815530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2923153.1"
                     /translation="MPQQSAETIIQAEKLAKKYGDLVAVDGIDFGIRRGETFGFLGPN
                     GAGKTTTMKMIYCFMPPTWGKLSVAGYDVTRNPREIKALLGVVPQEDSLDPDLTVYGN
                     LLAYSRYYDISPKVAHERIVELLDFMQLTEKKNTIIYALSGGMKRRLVLARGLINSPQ
                     ILVLDEPTTGLDPQARHLVWQKLRHLKSQGLTILLTTHYMEEASLLCDRLVIMNAGKI
                     LDSGSPQELVKKVVGQEVLEIYSADDALFQKVRSSVGPRATAELFGDVIYLYSNDGQP
                     ILSELPRLGIENFLHRRATLEDVFLRLAGRTLKE"
     gene            complement(1227..3344)
                     /gene="acs"
                     /locus_tag="AC16GM_05425"
     CDS             complement(1227..3344)
                     /gene="acs"
                     /locus_tag="AC16GM_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0046356 - acetyl-CoA catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase alpha subunit"
                     /protein_id="HIR2923154.1"
                     /translation="MKDMPHPLDYIFRPRSVAVIGASTQKGTLGREILHNIIEYEFNG
                     KVFPVNPRATVIHSIKCYSTILDVPDAVDLAVIAVPRDAVLPVVDQCGQKGVKGLVVI
                     TAGFKETGPKGAELERQLGGKVADYGMRMIGPNCFGVINTEPEVRLNATFSKTKPLGG
                     TIAFESQSGAMGEAILYHAQQINLGFSMFASVGNKTNVSGNDLLQYWRDDPNTKMILL
                     YLENFGNPRKFTQIARQLTRKKPIIAVKSGRTMQGARAATSHTGALAGLDVGVDALLS
                     QCGVIRASSIEEMFDTARAMATQPFPRGKRVAIVTNAGGPGIMTTDACVNLGLEIVHL
                     TPETKKKLREFLPEEASVENPVDMIASATAERYGKTLAAVLADPQVDAVIVLCVPPIN
                     VNAVEVAQAIISVRKNFPDKPLLASFMGVAEHSPGTELLIANGIPAYTYPESMVKALA
                     DLLKYRQWLARPEGQVKVFSVKKVQAEAIITEATSNGQKQLVGEPALNLLAAYGIPIA
                     PYAAAATPEEAVQLAAQVGFPVVLKLNSPKLLHKTEVGGVLVDLRGEKEVSEGFRTLE
                     KRYRQASGDGDFSGVVVQQMIKSGVETVIGMVSDPGFGPLMMFGLGGIYVEVLKDVAF
                     KIHPLTEADAWEMVKSLKSYPLLAGFRGAKPVDLEELVNAILKLSQLVSDFPQIDQFD
                     INPFIVSPRKDATRAVDARFLLR"
     gene            complement(3482..4144)
                     /locus_tag="AC16GM_05430"
     CDS             complement(3482..4144)
                     /locus_tag="AC16GM_05430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2923155.1"
                     /translation="MRGAARVQRLFDRTAEKFDTFYAEKKSPFKRFIDQHFHRDIFER
                     FELTFQHAQPIGRKTVLDVGCGSGLYCIEFARRGARRVVGIDFAPKMLELARAAAREQ
                     KVGDLCKFIQAEFLAYSFREQFDYTVALGVFDYLREPIPFLQKMRDVTREKILISVPS
                     VHWLRTPIRKFRYLFKRAPVVLYSRRKLEKMLREAGCTTFEIIKIPGAGMDFFVAINP
                     CG"
     gene            complement(4141..>4417)
                     /locus_tag="AC16GM_05435"
     CDS             complement(4141..>4417)
                     /locus_tag="AC16GM_05435"
                     /inference="COORDINATES: protein motif:HMM:NF023385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HIR2923156.1"
                     /translation="KNLPACGGGYFRLFPYAYTRLAFRQVEGLGQPVMFYTHPHELDV
                     EESNLYRSQTPFKKRFTQFTGRRGMEQKLHRLLSEFCFAPARKVLQV"
BASE COUNT          912 a         1299 c         1273 g          933 t
ORIGIN      
        1 gatgtccagt gagccaagtt tcatatcatc ggtttcctat catagaaccg gccttaaggc
       61 cggttcggga taacgatttt ttccgcgctc cggctatcac gagtaatcct tcagaaccgc
      121 ccttaagggc ggttctgtac tttctaaagc acgcatcatc ggcccatcct caatcactct
      181 tttaacgtcc tccccgccaa tctgaggaag acatcctcta acgtcgcccg gcggtgcagg
      241 aagttctcga tgcctaaccg cggcagctcc gagagaatcg gctgaccgtc gttggagtag
      301 aggtagatga cgtcgccgaa caactccgcc gtggcgcggg ggccgacgct tgagcgcact
      361 ttctggaaga gcgcgtcgtc cgcgctgtaa atttccagca cctcctgccc caccactttt
      421 ttgaccaact cctgcgggct gccactgtcc aaaattttac cggcgttcat aatgaccagc
      481 cggtcgcaga gaagactcgc ttcttccata tagtgggtgg tcaagaggat cgtcaacccc
      541 tgacttttca gatggcgcag tttctgccag actaaatgcc gcgcctgagg atccagcccc
      601 gtggtcggct cgtccaacac cagaatctgc ggactgttga tcaacccccg ggctaaaacc
      661 agacgcctct tcatccctcc ggagagggca tagatgatgg tattcttctt ctcggtcaac
      721 tgcatgaaat cgagcagttc aacgattctc tcgtgcgcca cttttgggga tatatcgtag
      781 taacgtgaat aggccaggag gttgccgtag acggtgagat cgggatcgag cgaatcctcc
      841 tgcggcacca cgcctaaaag ggctttaatt tcacgcgggt tccgggtgac atcgtacccg
      901 gctaccgaaa gtttccccca ggtcggcggc atgaagcagt aaatcatttt catcgtggtg
      961 gtcttgcccg cgccattggg gcctaggaag ccgaaggtct ctccccggcg gatgccgaag
     1021 tcgatgccgt ctacggccac caggtcgccg tactttttgg ccagtttctc ggcctggatg
     1081 atggtctctg cagactgctg tggcatatca ggactaattt acacaaccag caccagaaaa
     1141 aaagccggcc gggtgaggct ctcccctact caccagtgga acaatgatgg ctatcggcct
     1201 atgagtagag caaccgctga cagagtctaa cggagcaaaa accgcgcgtc gaccgcccgg
     1261 gttgcatctt tgcggggtga aacgatgaac gggttgatgt cgaattgatc tatctgcgga
     1321 aaatcggaca ccagctggga aagtttcaga atggcgttga cgagttcctc cagatcaacc
     1381 ggtttggcgc cgcggaaacc ggccaggagc gggtaactct tgagcgattt gaccatttcc
     1441 caggcatccg cttcggtcag cgggtgaatt ttgaaggcaa cgtctttgag cacctcgacg
     1501 tagatgccgc ccaggccgaa catcatgagg ggaccgaagc ccggatcgct caccatcccg
     1561 attaccgtct cgacgcctga ttttatcatc tgctgcacca cgaccccgga gaaatcacca
     1621 tcaccggagg cttggcggta ccgtttttcc aacgtgcgaa agccctcgct cacttccttt
     1681 tcgccccgga ggtctacgag gacgccgccc acctcggtct tgtgcaggag tttgggtgag
     1741 ttgagcttca aaactaccgg gaaacccact tgtgctgcga gctgcaccgc ttcctcgggc
     1801 gtggccgccg ccgcgtaagg cgcgatgggg atcccgtacg ccgccaaaag attgagcgcc
     1861 ggttccccga ccaattgctt ctggccatta gaggtggctt cggtgatgat tgcctccgcc
     1921 tgcacttttt tgacggagaa aactttcacc tgcccttcgg gccgcgccag ccactgccgg
     1981 tacttgagca ggtcggctaa ggccttgacc atcgactccg gataggtgta ggccgggatg
     2041 ccgttggcaa tcaaaagttc cgttccgggg gagtgttccg ccactcccat aaacgaggcc
     2101 aaaagcggct tatcgggaaa gttctttctg actgaaatga tcgcctgtgc cacttccacc
     2161 gcgttgacgt tgatcggcgg cacgcagagg acgatcacgg catcgacctg cggatcggcc
     2221 agaacggcag ccagcgtttt gccgtagcgt tccgccgtag ccgaggcgat catatcgacc
     2281 ggattttcca ccgaggcctc ttccggcaga aactcgcgca gcttcttttt ggtttccggc
     2341 gtcaggtgga caatttccaa gccgagattg acgcaggcgt cggtggtcat aatacccggt
     2401 ccgccggcgt tggtgacgat cgccacccgc ttgccccggg ggaagggctg ggtggccatg
     2461 gcccgggcgg tgtcgaacat ctcctcgatg gacgaggccc ggatgacccc gcactgggag
     2521 aggagcgcat ccactccgac atccaacccg gccagagcgc cggtgtgcga ggtggccgcc
     2581 cgcgccccct gcatcgtccg gcccgatttg accgcgatga tcggcttctt gcgggtgagt
     2641 tgccgggcaa tctgggtgaa tttgcgcggg ttgccgaagt tctccaggta gagaagaatc
     2701 attttcgtat tcggatcgtc ccgccagtac tgcaacaggt cgttgccgga gacgttggtc
     2761 ttgttcccca ccgaggcgaa catcgaaaac cccagattga tctgctgggc gtggtagaga
     2821 attgcctcgc ccatggcgcc cgactgggac tcaaaagcga tggtaccgcc cagcggcttg
     2881 gttttgctga aagtggcgtt cagccgtacc tccggttcgg tgttgatgac cccgaagcag
     2941 ttcggcccga tcatccgcat cccatagtcc gccaccttcc cgcccagctg ccgttccaac
     3001 tccgctcctt tgggtccggt ctccttgaac ccggcggtga tgaccacgag ccccttgacc
     3061 cccttttgcc cgcactgatc cacaaccggc agcaccgcat cccggggcac ggcgataacc
     3121 gccaagtcaa ccgcgtccgg gacgtcgagg atggtggagt agcacttgat ggagtggatg
     3181 accgtggccc tgggattgac cgggaagact ttgccgttga actcgtactc gatgatgttg
     3241 tgcaagattt cccggcccag ggtgcccttc tgggtggagg cgccgatcac ggccaccgag
     3301 cgcgggcgga agatgtagtc aagcggatgg ggcatatctt tcattgaccc tcaaaagaat
     3361 ctacgcagag aaagttcgcc gaacaaacct cgaaagcgga gggatcgtca ttcacgacgg
     3421 tcgggagacc gtcgctacaa aaactttccg caagtcacag agcgacgcaa atgaagagaa
     3481 attagccgca gggattgata gccacaaaaa aatccattcc cgctccggga attttgatga
     3541 tctcaaaggt tgtgcatccc gcctcgcgca gcatcttttc caatttcctg cggctgtaga
     3601 ggaccactgg cgcccgtttg aacaggtagc gaaacttgcg aatcggggtg cgcaaccagt
     3661 ggacggacgg cacggaaatc aggatttttt cacgcgtaac gtcccgcatt ttctgcaaga
     3721 acggtatcgg ttcccggagg taatcgaaca cgcccagcgc cacggtataa tcaaattgct
     3781 cccgaaacga gtaggcaagg aactcggcct ggatgaactt gcaaaggtcg cccacctttt
     3841 gctcccgagc cgcggcccgt gccagttcaa gcatcttcgg tgcgaaatcg atacccacca
     3901 cgcgccgggc gccgcgccgg gcgaactcaa tgcagtagag gccggagccg cagcccacat
     3961 ccagcaccgt ttttctcccg ataggctggg cgtgctggaa ggtgagctcg aagcgctcga
     4021 agatgtcgcg gtggaagtgc tggtcgatga atcgcttgaa ggggcttttc ttttcggcgt
     4081 agaaggtgtc gaatttttca gcggtgcggt cgaagagccg ctggacccgg gccgcgcccc
     4141 tcatacctgg agcaccttcc gggccggggc gaagcaaaac tcggagagca aacggtgcag
     4201 tttttgttcc attcccctgc ggccggtgaa ttgggtgaaa cgcttcttaa acggtgtttg
     4261 ggagcggtag aggtttgact cctcgacatc aagttcgtgt gggtgggtgt agaacatcac
     4321 cggttggccc aggccttcaa cctgtcggaa cgccagccga gtgtacgcat acggaaagag
     4381 ccggaaatat ccccccccac aggcggggag gtttttc
//
LOCUS       DBNGZD010000204         6657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1764717, whole genome shotgun sequence.
ACCESSION   DBNGZD010000204 DBNGZD010000000
VERSION     DBNGZD010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1764717"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2483)
                     /locus_tag="AC16GM_05440"
     CDS             complement(<1..2483)
                     /locus_tag="AC16GM_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2923157.1"
                     /translation="MYLSNLSIRRPVFATMLIVALLVLGVTSYFQLNVDLFPEVDFPV
                     VAITTAYPGAGPEAVEAEVTKKIEDAVNPVAGIKHIASTSQEGFSTIIAEFTLETRIQ
                     DAAQEVREKVAAIRAFLPKEIEEPVIARFDPASQPIISLAISGERSLRDLTTYTNNKI
                     KKRLESIPGVRSVELVGGAEREIQVALAKSKLVAHDLPVEKVVSSIASANIEIPGGKV
                     GKGQREALLRTLGRFDPAGGAVPALQKVIVSTRQGRYVRLGEIAAVADSVKEPTSLSS
                     LNGREAVGLDITRQSGANTVAVASAVKAELARLEKELPPEMKIILAVDRSVFIHDAID
                     DILVNIYYGGALAILVIFLFLANWRSTLISALAIPTSIIATFTLMRLLHFTVNFMSLL
                     GLSLAVGLLIDDAIVVIENIYRHLDRGESAREAAKNATAEIGLAVMATTFSIVVVFLP
                     VAFMSGIVGRFFYQFGMTVAAAVLVSLFVAFTLTPMLSSRLLRPETKAERRGFFWKIP
                     LGWNAAFQKLNGVYRRVLAWALGHRKTTVAIALGTFVASFILVPFMGVEFIPQSDRGE
                     FVVNFETPTGSSLAHTAAVAEKIEQRIRRHPEVTTVLTTIGAGQNEVHQGRINVKLVE
                     RSKRKAGVFTFMNTLRWELAGLPGIKLAIGTEGMAGRTAKQIELSVRGDDPVLLRRLA
                     GRVEEIVRRTPGTVDVDNSEKAGKPELRVAINRDKASDLGLSPRSIAATLRTLVDGEV
                     ATKYQEGDEQYDVRVRLAEGDRSNPAAIAQLAVASTKEIEGKKDFLVPLNQVATLVEE
                     TGPTTINRYDRQREILITANVSGHVFGNV"
     gene            complement(2533..3582)
                     /locus_tag="AC16GM_05445"
     CDS             complement(2533..3582)
                     /locus_tag="AC16GM_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2923158.1"
                     /translation="MKKSLKPMAATVTGLLFLFASAGCGEKGNAEKDAVPAEKVPVEL
                     ASVLPRDLHLTLTCSGSLEGFEQATLYGKIPEVVTAIKVKEGQRVTKGQLLIELEKAG
                     PHSNYLQSQAIYLNAEKNYQKLENLYQQKAVSEQARDQAKTNYEVALANFNGARDLVE
                     IKSPIGGQVTEIDLVEGQQVPVGQVIAVVAKTAPLRLKFAVSYAEVKLLKPGQSVRVT
                     SELQPGKWVSGSIAEVSGSADPVTRTFQVEALLANPEGLFKPGMFAQGEAAVGTLKGA
                     LAIPKEAVVLREGRPYVFAVNSDVAKLVAVETGAEAGGLVEIRSGLKAGDQVVTAGQS
                     NLFDGLPVRRAETAE"
     gene            complement(3604..4896)
                     /locus_tag="AC16GM_05450"
     CDS             complement(3604..4896)
                     /locus_tag="AC16GM_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2923159.1"
                     /translation="MSLSTLAEPLPLTLDDALGQAKEKNESYLIAQQEIKKARAQIRE
                     ARSGALPTLTLSSTYTRNWQLPAAVFDIKGERQRIRLGADNSYLANLTLQQPLYVGGK
                     VGAALKIARYYEAVVKARAREVEAALELEVKRAFYDAVLAQDLVTVAQEALRQAESNR
                     DVVAKLFKEGMVAEFELLRAEVQVANLKPQLLKARNGADLALHNLKTVIGAPLAQEIA
                     LVFQFPQARVARVYDYDSLLAQALALRPEVKQLEDQVKIQSQLIGIAQGGYRPSLFAS
                     TSYQFQAQSNRFVPDGRDWIHSWNSALLIQIPIFDGFKTPAEVQQAKIDYRESQFRKK
                     QLEDQVALEVRQALGNLNEGRERILAQEKAVDLGRTGLSIAEVRYQNGVGTQLELIDS
                     QVALTTAQTNQVQAFHDFLVAEAQLARAAGTELVHGEE"
     gene            complement(5010..5510)
                     /locus_tag="AC16GM_05455"
     CDS             complement(5010..5510)
                     /locus_tag="AC16GM_05455"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR2923160.1"
                     /translation="MVAVKELMLNNVNVVNVGYMAPTFTLLDQKGTACTLLDEMGRHH
                     VVLVFCRGSWCPASAQHLIALNQVQNEFAQRGAVVWAVSAERQRDTQRFAKKEKINFP
                     LLEDKNLEVITRWGVRSSNSARPVPHPATFLIDIDGIVRLKEVSVNEVRYSPPSRLIE
                     QLALLA"
     gene            complement(5650..>6657)
                     /locus_tag="AC16GM_05460"
     CDS             complement(5650..>6657)
                     /locus_tag="AC16GM_05460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR2923161.1"
                     /translation="QMSVLLATLKKYQRIWGPLAALLFLALCVYDLDFNLVWSAIQRV
                     HYGYLVPSVFSCFIFALLRSQRWRMIIDPRKPISRRRIFRLFMIGQLANAALPALTGQ
                     AVRIWLLSKKEGLTKTFAFTTVVLEVVFDALSLLLLLAGFSSLLVLPRWLVRGELAVA
                     LGITVLFVLFYFFLRERPPVWMEKYLITIPAHRVKKLNDLYHSVSSGLAMLKSTRHLF
                     SVFGLSLLAWLAQTGTVFFLMEAFGLEAPLEAALLVIVINTVALIIPITPGNVGTFQL
                     ACTFALSLFAVPKAEAFSFSVLLQVVDLLPVVTLGVYFLVAEHLRVRDLKSAEVLEYR
                     P"
BASE COUNT         1387 a         1979 c         1935 g         1356 t
ORIGIN      
        1 atcacgttgc cgaagacatg gccggacacg ttggcggtaa tgaggatttc acgctgccgg
       61 tcgtagcggt tgatggtggt ggggccggtc tcttccacca gggtcgccac ctggttcagc
      121 ggcaccagaa aatctttttt cccctcaatt tctttggtac tggcaacggc gagttgcgca
      181 atggcggccg gatttgagcg gtctccttcc gccaggcgga cccgcacgtc gtactgctcg
      241 tccccctcct ggtacttggt cgccacttcc ccatccacta aggtccgcaa cgtggccgca
      301 atcgagcgcg gcgacaatcc taagtccgac gctttgtccc ggttgatcgc cacccgcagt
      361 tccggtttgc cggccttctc gctgttatcg acatcgaccg tccccggcgt gcgtcggacg
      421 atctcttcca cccgacccgc caggcggcgc aaaagaaccg gatcgtcgcc gcgaacggaa
      481 agttcgattt gcttggcggt acggcccgcc attccctcgg tgccgatggc cagtttgatt
      541 ccggggaggc ccgccagctc ccagcggagg gtgttcatga aggtaaaaac acccgccttc
      601 cgtttactcc gctcaaccag cttgacgttg atccgcccct ggtggacttc gttctggccg
      661 gcgccgatgg tcgtgagcac ggtagtcact tcggggtgcc ggcgaatccg ctgttcaatt
      721 ttttcggcca ccgccgcggt gtgggctaag gacgacccgg tcggcgtttc aaagttgacc
      781 acaaactcgc cccgatcgga ctggggaatg aactccaccc ccatgaaggg gaccaggatg
      841 aaactggcca caaaggtccc caaagcaatc gccaccgtcg tcttgcgatg ccccagggcc
      901 caggccaaaa cccggcggta gacgccgttt aatttctgaa acgcggcatt ccagcccaaa
      961 ggaattttcc agaaaaatcc ccggcgctcc gcctttgttt ccggtctcaa aagacgcgag
     1021 gagagcatcg gcgtgagagt gaaagcgacg aagagggaga ctaaaaccgc agcggcgacc
     1081 gtcatgccga actggtagaa gaaacggccg acgatccccg acatgaacgc caccggcagg
     1141 aagaccacca caatggaaaa ggtggtggcc atgacggcca ggccgatttc ggcggtcgca
     1201 tttttggccg cttcccgggc cgactcgccc cggtctaagt gccggtagat attttcgatc
     1261 acgacaatgg cgtcgtcaat caaaagcccc acggccagag agaggccgag caaactcatg
     1321 aaattcaccg taaaatggag cagccgcatc agggtgaagg tggcgataat cgacgtgggg
     1381 atggccaggg ccgaaatgag cgtgctccgc cagttagcca aaaagaggaa aatgaccagg
     1441 atggccagcg ccccgccata gtagatattg accagaatgt cgtcgatggc atcgtggata
     1501 aagacggagc ggtccaccgc caaaatgatt ttcatttcag gggggagttc cttttccaac
     1561 ctggccagtt cggctttaac cgcgctggcc accgccaccg tattggcgcc cgactgccgg
     1621 gtaatatcga ggccgactgc ctcccggccg ttgagactcg agagggaagt cggttccttc
     1681 acgctgtcgg ctaccgcggc gatctccccc agccggacgt acctgccctg acgggtggag
     1741 acgatgacct tctgcagcgc cggcaccgcc ccccccgccg gatcaaaacg gcccagggtg
     1801 cgcaagagcg cctcccgctg gcccttgccc accttcccgc cgggaatttc aatgttggcc
     1861 gatgcaatgg aactgaccac tttttccacc ggcaggtcgt gggccactaa tttgctcttg
     1921 gccaacgcca cctgtatttc ccgctcggct ccgcccacca gttccaccga cctgaccccg
     1981 ggaattgatt cgagccgctt tttaattttg ttgttggtat aggtggtcag gtcgcggagg
     2041 gaccgctcgc ctgagatcgc gagcgagatg atgggctggc tggcggggtc aaaacgggcg
     2101 atcaccggct cttcgatttc tttggggagg aacgcccgga tggcggccac cttctcccgg
     2161 acttcctgcg ccgcgtcctg gatccgggtt tcgagcgtga attcggcaat gatggtggaa
     2221 aacccctcct gagaagtgga agcaatgtgc ttgatcccgg cgaccggatt gacggcatct
     2281 tcgatcttct tggtcacctc ggcctccacc gcctccggtc cggcgccggg ataggccgtg
     2341 gtgatcgcca ccaccgggaa atcaacttcg gggaagaggt cgacattgag ctggaagtag
     2401 gaggtaacgc ccagaaccag aagggccaca atcagcatcg tggcgaagac cggccggcgg
     2461 atggaaaggt tagagagata catggttcac ggcgcttttg ggaaaaattc ctgaattaat
     2521 catccttgcg agttactctg cggtttccgc ccggcgcacc ggtaagccgt cgaaaaggtt
     2581 actctgcccc gccgtgacga cctggtcgcc ggctttgagg ccggagcgaa tttcgaccaa
     2641 accgcccgct tcggcgccgg tttcaaccgc caccagtttg gcaacatcgg agtttactgc
     2701 aaaaacgtag ggccttccct cccgcagcac gaccgcttcc ttgggaatgg cgagtgcgcc
     2761 tttcaaggtg cccaccgccg cttcgccctg ggcgaacatc cccggtttga acagcccttc
     2821 gggattcgcc aggagtgctt cgacctggaa ggtgcgcgtg accggatcgg ctgagccgga
     2881 gacttcggca atgctgccgg agacccactt cccgggctgg agttcggagg tgacccgcac
     2941 gctctggccc ggtttgagga gcttcacttc tgcataactg acggcaaact tgagccgcag
     3001 gggtgcggtc ttggccacca ccgcaatgac ctgccccacc ggcacctgct ggccttccac
     3061 cagatcgatt tcggtgacct gacccccgat gggcgatttg atttccacaa ggtcccgcgc
     3121 gccattgaag ttggccaacg ccacctcgta gttggttttg gcttggtcgc gggcctgctc
     3181 ggagaccgct ttctgctgat acaggttctc cagcttctga tagttcttct ccgcgttcag
     3241 ataaatcgcc tgggactgga ggtagttgga gtggggcccg gctttttcca gttcaatgag
     3301 cagctgcccc ttggtaaccc gctgcccttc tttaacctta attgccgtca ccacttcggg
     3361 aattttgccg tacagcgtcg cctgttcgaa cccctcgagg ctgccagagc aggtcaaagt
     3421 caaatgcaga tcgcggggca agacactggc cagttctacc ggcacttttt cggcgggcac
     3481 cgcgtctttt tccgcattgc ctttttcgcc acacccggct gaggcgaaca ggaaaagaag
     3541 cccggtgacc gtcgctgcca ttggctttag actttttttc ataaggctac ccttcttcat
     3601 tccttactct tccccgtgaa ccagttccgt gccggcggcc ctggcgagtt gcgcttcggc
     3661 caccagaaaa tcgtggaagg cctgtacctg attggtctgg gcggtggtca aggccacctg
     3721 tgaatcgatc agttccaatt gcgtccccac cccgttctgg tagcgcactt cggcgatcga
     3781 caaaccggta cgccccaggt caaccgcttt ctcctgggct aaaatccgtt cccggccttc
     3841 gttcaaattc cccagcgcct gccgcacctc caaggccacc tgatcttcga gctgcttttt
     3901 gcggaactgc gactcgcggt agtcaatctt ggcctgttgc acttcggccg gagtcttaaa
     3961 tccgtcgaaa atggggatct ggatcaaaag cgcgctgttc caggagtgga tccagtcccg
     4021 gccatccggg acgaaccggt tcgattgcgc ctgaaactgg taggaggtgg aggcgaaaag
     4081 cgacgggcgg tagccgccct gggcgatgcc gatcagctgg ctttgaattt tgacctggtc
     4141 ttctaattgc ttcacttcgg gccggagcgc caaagcctgc gccaaaagtg agtcgtagtc
     4201 gtaaacccgc gcgacccgcg cttgggggaa ctggaagact aaagcaattt cttgtgccaa
     4261 gggcgcgccg atgaccgtct tgagattgtg caaagccaaa tcggcgccgt tccgggcttt
     4321 gagcaattgc ggtttgaggt tggccacctg cacctcggcc cgcaagagtt cgaactccgc
     4381 caccattcct tccttaaata acttggccac tacatcccgg ttcgactcgg cctggcgcaa
     4441 ggcttcctgc gccacggtga ctaagtcttg cgccagaact gcgtcgtaaa aggcccgctt
     4501 gacctcgagt tccagtgcgg cttcgacctc gcgggcccgg gctttgacca cggcctcgta
     4561 gtaccgggca atcttgagcg ccgccccaac tttgccgccg acgtagaggg gctgctggag
     4621 ggtcaggttg gcgaggtagc tattgtccgc ccccaagcgg attctctgtc gctccccttt
     4681 gatatcaaag actgcggccg gcaactgcca gttgcgggtg taagtgctgg agagcgttaa
     4741 agtcggcagg gcgccggagc gagcttcccg gatctgcgcc cgggcttttt tgatttcctg
     4801 ctgtgcaatc aggtaggatt catttttctc tttggcctgg ccgagggcgt catccaaggt
     4861 caaaggtaaa ggctcggcaa gggttgaaag ggacaaaaaa attaccccag cactcatagt
     4921 gaaaaatgcc cttatgaccc atatcatatt agtgaataat ttcacaaaaa tccctacgca
     4981 aaataaggtt ctgtgtttgt ctggataagc tacgctaaaa gggccaactg ctcgatgaga
     5041 cgactcggcg gcgaatagcg gacttcattg acggaaactt ccttgagcct gacgatgccg
     5101 tcaatgtcga tcaaaaaggt cgccggatgg ggcaccggcc gggcggagtt ggaactcctt
     5161 actccccagc gggttattac ttccaggttt ttgtcttcta aaagtgggaa gtttatcttt
     5221 tccttcttcg caaacctctg ggtgtcgcgc tgcctttcgg cggataccgc ccagacgact
     5281 gcgccgcgct gggcaaactc gttctgcacc tggttaaggg cgattaaatg ttgagcggag
     5341 gccgggcacc aggaaccgcg gcagaagact aaaacgacgt ggtgtctccc catttcgtca
     5401 agtaaggtgc acgccgttcc tttctgatct aaaagtgtga aggttggggc catgtagccg
     5461 acgttgacaa cattgacgtt gttcagcata agttctttga ctgcaaccat cctctgcctc
     5521 ttggaaaagc tacattcctt attgggccat accaatatgc gccctgttcc aataccgtca
     5581 agcctccggt gccaccagcg cacgcggcga ggcgccgcgc ctgcgtgcgg gtctggagca
     5641 ggcaaacgtt tacggtcggt actccaatac ttcggcgctc ttcaggtcgc gcacccgcag
     5701 gtgctcggca accagaaaat agacccccag ggtcaccacc ggcaaaaggt ccacgacctg
     5761 gagtaagact gagaaactga acgcttccgc tttcggcacc gcaaaaaggg agagcgcaaa
     5821 ggtgcaggcc aactggaagg ttccgacatt gcccggggtt atgggaatga tcaaagccac
     5881 ggtattgatg acaatgacta aaagcgccgc ttccagaggc gcctccagac cgaaggcctc
     5941 catcaaaaag aagaccgtgc cggtctgcgc cagccaggcc aaaagcgaca acccgaacac
     6001 actaaagagg tgccgggtgc tcttgagcat ggcgagacca ctggacacgg agtggtagag
     6061 atcgttcaac ttcttgacgc ggtgcgccgg gatggtaatg aggtacttct ccatccagac
     6121 cgggggtcgt tcgcgcagga aaaagtaaaa gaggacaaaa aggacggtga tgcccagagc
     6181 caccgccagt tcaccccgca cgagccagcg gggcaggacc agaagcgacg agaatccggc
     6241 gaggagcaaa aggagactca gggcgtcgaa gaccacttcc agaaccacgg tggtgaaagc
     6301 gaaggtcttg gtcaggccct cttttttgct caagagccag atgcgcaccg cctggccggt
     6361 gagggccggc agggcggcat tggccaactg gccgatcatg aagagccgga aaatgcggcg
     6421 ccgggagatc ggcttgcggg gatcgatgat catccgccac ctctgactgc gcaggagggc
     6481 gaaaatgaaa caagagaaaa ccgagggcac gaggtacccg tagtgcaccc tctggatcgc
     6541 cgaccagacc aggttgaaat ccagatcgta gacgcagagg gccaaaaaga ggagcgccgc
     6601 cagggggccc cagatccgct ggtacttttt caaagtcgcc aggagcacgg acatttg
//
LOCUS       DBNGZD010000205         2711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1629683, whole genome shotgun sequence.
ACCESSION   DBNGZD010000205 DBNGZD010000000
VERSION     DBNGZD010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1629683"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC16GM_05465"
     CDS             complement(<1..365)
                     /locus_tag="AC16GM_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR2923162.1"
                     /translation="MTLLSKHLYIETYGCQMNEADAELIYGHLASSGYRKTKSLTEAD
                     VILVNTCAVREHAEQRVLGRVSQLAALKTGNPDLILGLVGCMATHLKEKLLDRAPFLD
                     LVVGPDSYRRLPQIIEEAA"
     gene            complement(437..994)
                     /locus_tag="AC16GM_05470"
     CDS             complement(437..994)
                     /locus_tag="AC16GM_05470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2923163.1"
                     /translation="MIITVKPLDDSLRNDFFTLHSDPAFGWCFCVAWEVPTWDGWGER
                     TAEQNRTLRENLFNQSRFDGYLLYVDGQPVGWCQCGPLEHWPKLYQQYQLKPEPKDYA
                     VTCFCLLPQYRKQDLSHILLREVLAHLKKRKVARVFAFPRKGENPDDDVWTGPEKIFL
                     SAGFTLWREAGKRVVLVRQLKSTAP"
     gene            complement(1271..2551)
                     /gene="mtaB"
                     /locus_tag="AC16GM_05475"
     CDS             complement(1271..2551)
                     /gene="mtaB"
                     /locus_tag="AC16GM_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR2923164.1"
                     /translation="MQKNSIFLQPKTCALFTVGCRLNQYETEAIGQQLEQAGYERVDF
                     GKPADLYVVNTCTVTAGADASSRQAIARAARYKGEGKLIVTGCYAQVARETVAKLPGV
                     DAVFDNYRKDNFLQLLSEWNGLDGQEPAEPVVVKGHRAHTRGLVKIQDGCNEWCAYCV
                     IPMSRGDSRCRPAEQITAEVRSLVEHGFKEVVITGVHIGKYRHDELDLVGLLRRILDE
                     TDVARLRLSSIKPNEVTPELIDLIASSGRICRFVHMPLQSGDDTTLKRMKRSYDCATY
                     LRIAERLKEKIPGLTIGADVIVGFPGETDEQFENGARFIEDSPIDYLHVFSYSDRPNT
                     AASRMPDKVPPQVKDHRSFVLRQLSDKKWKCHLDRFIGQRMPVLVEENGKVASCKTAI
                     ADNFIRVLLPENGYKENSIVEVIPNKREGKSLVA"
BASE COUNT          576 a          764 c          784 g          587 t
ORIGIN      
        1 ttggctgctt cttcgatgat ttgcggcagg cggcggtagg aatcgggtcc gacgactaaa
       61 tcgagaaacg gtgctctgtc caaaagtttt tctttcagat gcgtcgccat acagccgacc
      121 agccccaaaa tcagatcagg gtttccggtt ttgagcgccg ccaactgcga cacccggcct
      181 aaaacccgct gctccgcatg ctcccgcacc gcgcaggtat tgacgaggat gacatctgcc
      241 tcagtgagcg atttggtctt ccgataccct gaggacgcca ggtggccgta gatcagttcg
      301 gcgtcggcct cattcatctg gcagccgtag gtttcgatgt agaggtgttt agataggaga
      361 gtcattgagg gatagtttac atccccacac tcaaaacgca agaccccacc ctgccccccc
      421 tacaggggag ggaaattcat ggggctgttg atttcagttg tcgcaccagc acaacacgtt
      481 tccccgcctc gcgccagagg gtaaatcctg cggagagaaa aatcttctct ggccccgtcc
      541 agacatcgtc gtcgggattt tctccttttc ggggaaaggc aaagacccgc gccaccttcc
      601 gttttttcag atgagcgaga acttccctca agagtatgtg gctcaaatcc tgctttcgat
      661 actggggaag caagcaaaaa caggtgacgg cgtaatcttt gggctctggc ttgagttgat
      721 actgttgata gagcttcggc cagtgctcga gcgggccgca ctggcaccag ccgacgggtt
      781 gaccatcgac gtagaggagg tagccgtcaa agcggctctg gttgaaaaga ttttcgcgta
      841 gagttcggtt ctgctcggca gtccgctcgc cccatccatc ccaggtgggc acttcccagg
      901 ccacgcagaa gcaccagccg aaggcagggt cggagtggag ggtgaagaag tcgttgcgga
      961 gggagtcgtc taagggtttg acggtgatta tcacggcgtt ctcagttgac ccctccctac
     1021 cctcccctca aggggagggt aagttgctcc ctctcctaat aggagagggt cgggatgagg
     1081 tcagcatttt ccctctcctt caggggaggg tgtcccgcca aaggcgggac gggaggggtc
     1141 ggaggtatgc gttatgactt tgcctgcggg ctgacccctc cctaccctcc cccgcccata
     1201 tgggccgtac ggccagaggc ggggtcttcg accctcatag tgaagggaat ctcagtggaa
     1261 actttatctc tcaggcaacc aagctcttcc cctctcgctt gttcggaatc acctccacaa
     1321 ttgaattctc tttgtagcca ttctcgggga gaagcacccg gatgaagtta tcggcgatgg
     1381 cggtcttaca ggaagcgact ttgccgtttt cctccaccaa caccggcatg cgctggccga
     1441 tgaaccggtc aaggtggcat ttccatttct tgtcagataa ctggcgcagg acgaaactgc
     1501 ggtgatcttt gacctgaggc ggaactttgt cgggcatgcg cgacgccgcc gtattgggcc
     1561 gatcggagta ggagaagacg tgcaggtagt cgatggggga atcctcaatg aaacgtgcgc
     1621 cattctcgaa ctgctcatcg gtctcgccgg ggaagccgac gataacatcg gcgccgatgg
     1681 tcagcccggg aattttctct ttgagccgtt ccgcaatccg caggtacgtg gcgcaatcgt
     1741 aactgcgctt catccgcttg agggtggtat cgtcccccga ctgcaggggc atatggacaa
     1801 aacggcaaat ccgtccagac gacgcaatga gatcaatcag ttccggcgta acctcattcg
     1861 gcttgatgga cgaaaggcgc aggcgggcga catcggtctc atccaatatc cggcgcagga
     1921 ggccgaccaa atcgagctca tcgtggcgat acttgcctat gtggacgccg gtgataacca
     1981 cctctttgaa cccgtgctcg accagcgacc gtacctcggc tgtgatctgc tctgccgggc
     2041 ggcagcggga gtcgccgcgg gacatcggga tgacgcagta ggcgcaccac tcattgcagc
     2101 cgtcctgaat tttgaccaac ccccgcgtgt gggcgcggtg gcctttgacg acgactggct
     2161 cggctggctc ctgcccgtcg aggccgttcc actcgctcaa tagttgaagg aagttatcct
     2221 tgcgatagtt gtcgaagacc gcatcgacgc cggggagttt agcaaccgtc tcccgtgcca
     2281 cctgcgcata gcagccggtg acgatcagtt tcccttcgcc cttataccgc gccgcgcggg
     2341 caatggcctg ccgggacgag gcatccgccc ctgccgtgac cgtgcaggtg ttgaccacgt
     2401 agaggtcagc gggcttgccg aaatcgaccc gctcgtagcc cgcctgttcc aactgctggc
     2461 cgatggcctc cgtctcgtac tggttcagcc ggcagccgac ggtgaagagc gcgcaggtct
     2521 taggttggag aaatattgaa ttcttttgca tttcgtcaaa aataaacgta aaaaaggaaa
     2581 aaaggcaatc taaaacaaaa acatcaggaa gaatttctta ctgagaaagt cagcgcgagg
     2641 aatgctgaag aatgcgtcgc agttgattac tagagataac caaggcaatc cctgactcga
     2701 cttgctggtt a
//
LOCUS       DBNGZD010000206         3556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1004451, whole genome shotgun sequence.
ACCESSION   DBNGZD010000206 DBNGZD010000000
VERSION     DBNGZD010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1004451"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC16GM_05480"
     CDS             complement(<1..197)
                     /locus_tag="AC16GM_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923165.1"
                     /translation="MGERGRKFVLIGFFIVIGLLANTGIAKVMTSTVSYPSGSDTVSG
                     YLALPGGQGPFPALVVIQEWWG"
     gene            379..795
                     /locus_tag="AC16GM_05485"
     CDS             379..795
                     /locus_tag="AC16GM_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923166.1"
                     /translation="MRYEIKRLGFWSVIKVTFILYGILGFLLGIFYALFLAFFASLAE
                     YAGGQEFPGIFSGALLVFVPIFVAFIVAIGGVIQNGVLVGLYNLFVRAIGGIEVTVAA
                     LEPTPVVPTAGTAPVTYTFTPTQPPQPGQPPPMSAV"
     gene            792..2249
                     /gene="gltX"
                     /locus_tag="AC16GM_05490"
     CDS             792..2249
                     /gene="gltX"
                     /locus_tag="AC16GM_05490"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR2923167.1"
                     /translation="MKSVRVRIAPSPSGFLHVGTARTAVYNYLFSRHHGGAFILRVED
                     TDVARSSPEMVAGILEGLQWLGLTWDEGPVFQSRRFDLYRQYAEKLFATGQAYFCFCT
                     PEELEQKRSAAIAQKIAWKYDRTCRQLSDAERQKKLAAGVPKAMRLFVPEGETVFRDS
                     VYGELRRHNADTEDFVCLRSNGRPTYNFACVADDADMQISHVIRGNDHISNTFKQILL
                     YRALGLEPPEFAHLPLILGPDRAKVSKRHGAESVTEFRDRGFLPETVLNFLALLGWSP
                     GDEREILSRKELIDLFSLERVTQANPIFDMQKLEWMSGEYLRALPDEQLLDCVLPHLQ
                     AAGLVTQEAAQKKREWLVRLIPLLKERARTLVDFASVGRYFFTDQFDYDPQGVAKQFA
                     DPQAAERLAELAKRFRDLSLFNKETADEALRSYALALGVKPALLIHPTRLALTGVNMG
                     PGLFDIVATLGQAEVVKRLEKAVVFLRTQKPVSIR"
     gene            2273..2653
                     /locus_tag="AC16GM_05495"
     CDS             2273..2653
                     /locus_tag="AC16GM_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2923168.1"
                     /translation="MFKQVDYVMVMVSDMARSVAFYRDKLGLPLKFESKDWTEFQTGA
                     TTLALHGGGVGQPPKPPNPGASFAGTCSIGFTVPDVEKSFQDLKAKGVNFVMPPKRQE
                     DEDIILAVCTDPDGLPVSLAQHIQ"
     gene            2741..2814
                     /locus_tag="AC16GM_05500"
     tRNA            2741..2814
                     /locus_tag="AC16GM_05500"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2773..2775,aa:Gln,seq:ctg)
     gene            2941..>3556
                     /locus_tag="AC16GM_05505"
     CDS             2941..>3556
                     /locus_tag="AC16GM_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2923169.1"
                     /translation="MALKIGIVSEYYYPLLGGITENVYNTRVRLQERGHEVKIITANC
                     DGTRFAVHRSQDFTDANVIRIGRSLPIYSNSSFGRFTAGRQLRAKMKGILERERFDLL
                     HLHSPVTSSLPAVALLEAKCALVGTFHSYFDWNLVYSVFKGFIQKKGINRLDGKIGVS
                     QSSIDALRRYFDLEARIIPNGVDTTQFSPAAPRLEKFSDGKRNLL"
BASE COUNT          717 a         1053 c         1026 g          760 t
ORIGIN      
        1 ccccaccatt cttggatcac caccagagcc ggaaaagggc cctggccccc gggcagtgcc
       61 aagtagcccg aaaccgtgtc gctgccgctg gggtaagaga ccgtgctggt cataactttg
      121 gcaatccccg tgttcgccag aagtccaatg acaatgaaaa aaccgatcaa gacaaatttt
      181 ctaccgcgct ctcccatttt tcctccccgg ttgtataccc atttattctt actacgtcag
      241 aaaaaaagag atttcggggg ataaaagcaa gcgtcgctcc gaacctgagg aaagccacgc
      301 ctttacaaaa tctctgcaat ggtgcatact tgaccggcag tgaccgaaac ggagtgaacg
      361 tcagatcggg ggtacacgat gcgctacgag atcaaacggt tgggattctg gtcggtgatc
      421 aaggtgacct tcatcctcta cggcattctg ggattcctgc tcggcatctt ctacgctctg
      481 tttctggcat tcttcgccag tctggcggaa tatgccggag ggcaggaatt tccggggatt
      541 ttctccgggg cgcttttagt tttcgtgccg atatttgtcg ccttcatcgt ggccattggg
      601 ggcgtgattc agaacggggt tctggtggga ctctacaatc tctttgtgcg cgccatcggc
      661 ggcatcgagg tgaccgtagc cgctctggaa ccgactccgg ttgtcccaac ggccggtact
      721 gccccggtca cttacacctt tacgccgacg cagccgcctc agcccggcca gcccccgcca
      781 atgtctgccg tatgaagtcc gttcgcgtcc gcattgcgcc gtcgccttcc ggctttttgc
      841 acgtgggcac ggcccgcacc gccgtttaca actatctttt ctcccggcac cacggcggcg
      901 ccttcatttt gcgcgtcgaa gacaccgacg tggcccgctc ttcgcccgaa atggtggcgg
      961 gaattttgga aggcctccag tggctgggct tgacctggga cgaagggccg gtcttccagt
     1021 cccggcggtt cgacctgtac cgccagtatg cggagaaatt atttgccacc ggccaggcct
     1081 acttttgctt ctgcacgccg gaggaattgg agcaaaagcg cagcgccgcc atcgcccaaa
     1141 aaatcgcctg gaaatacgac cgcacctgcc ggcagttgag cgacgccgag cggcaaaaga
     1201 aattagccgc tggagttccg aaagcgatgc ggctctttgt gccggaaggg gagaccgttt
     1261 tccgcgattc agtctacggc gaactgcggc gtcacaatgc cgacaccgaa gatttcgtct
     1321 gcctgcgctc caacggaagg ccgacctaca acttcgcctg cgtggcggac gatgccgaca
     1381 tgcagatttc gcacgtcatc cgggggaacg accacatttc caacaccttc aaacagattt
     1441 tgctctaccg ggcgctgggt ttggagccgc cggagttcgc tcacctgccg ctcattttgg
     1501 ggccggatcg ggccaaagtt tccaaaaggc atggggctga gtcggtcacc gagttccgcg
     1561 accggggctt tttgcccgaa acggttttga atttcttggc gctgttaggc tggtcgcccg
     1621 gggacgagag ggagattctt tcccggaaag aattaatcga tcttttctcc ttagagcggg
     1681 tgacacaggc caatccgatc ttcgacatgc aaaaattgga gtggatgagc ggggagtacc
     1741 tgcgcgccct gccggatgag cagctgctcg attgcgtcct ccctcacctg caggccgcgg
     1801 gtctcgtgac tcaggaagcc gcgcagaaga agcgtgagtg gctcgtgcgc cttattccgt
     1861 tgctcaaaga gcgcgcccgg acgctggttg atttcgcttc cgtcgggcgc tacttcttta
     1921 ccgaccagtt tgactacgac ccgcagggcg tggccaagca gtttgccgac ccccaggcag
     1981 cggaacggct ggcagaactc gccaaaaggt ttcgcgacct ctctttattc aataaagaaa
     2041 ctgcggatga ggcactgcgc agctacgctc ttgcactcgg cgtcaaaccg gcacttttaa
     2101 tccatcccac ccggctggcg ttgaccgggg tcaacatggg gccggggctt tttgacatcg
     2161 tcgccacttt aggccaggcc gaagtggtca agcggttaga aaaggcggtt gtatttctgc
     2221 ggacgcagaa accggtctct atccgctgaa agtctcaaat gaggaggtag tgatgttcaa
     2281 acaggttgat tatgtcatgg tgatggtttc cgatatggcg cgttcggtcg cgttctaccg
     2341 cgacaaatta ggactgccgt tgaagttcga atccaaagac tggaccgaat tccagaccgg
     2401 cgcaacgaca ctcgctctgc acggcggcgg ggtcggtcag cccccgaagc cgcccaaccc
     2461 cggagcttcc ttcgccggca cctgctctat cggcttcacg gttcccgacg tcgagaaaag
     2521 tttccaggac ttgaaggcga agggcgtcaa ttttgtgatg ccgcccaagc ggcaggaaga
     2581 cgaagacatc attttggcgg tctgcaccga tcccgacggg ctgcccgttt ctctggcgca
     2641 acatatacag taagcggcgc cgacttgacc ggcgccgcct aagccgttga cggcgagcgt
     2701 cgtcgaaccg tatactctgg caagtgattt ttactcgacc tgggggatcg tctaacggca
     2761 ggacgagtgg ctctggacca cttaatcggg gttcgaatcc ctgtccccca gccaattttc
     2821 attttttttc ctttggccgt agtgccggaa gattttctgt gggtgacagg gaaggtcgtg
     2881 ccgtcgagcc cgccggttga ctgacgcagg tcgcgagcac ccggggagaa aacgccaacc
     2941 gtggctttga agatagggat cgtcagcgaa tactactacc cgcttctggg gggcatcacc
     3001 gagaacgtct acaacacgcg ggtgcggctg caggagcgcg gccacgaagt gaaaatcatc
     3061 accgccaact gcgacggcac gcggttcgcc gtccaccgga gccaggattt caccgatgcg
     3121 aacgtcattc gcatcggccg cagtctgccg atctactcca acagttcctt cggccgcttc
     3181 accgccggca ggcagttgcg ggcaaaaatg aaaggtatcc tggagcggga gcggttcgac
     3241 ttactccacc tgcactcccc cgtcacctct tctctcccgg cggtggcgct gctggaggcc
     3301 aagtgcgcac tggtgggtac gtttcacagc tacttcgact ggaacctggt ctattcggtc
     3361 ttcaagggat tcatccagaa aaaaggcatc aaccggctgg acggaaagat cggcgtttcc
     3421 cagagcagca tcgatgcgct gcgccggtat ttcgacctcg aggcgcgcat tataccgaac
     3481 ggcgtggata ccacccagtt cagccccgcg gcgccgcggc tggagaagtt cagcgacggc
     3541 aagcggaacc tcctct
//
LOCUS       DBNGZD010000207         4069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_588626, whole genome shotgun sequence.
ACCESSION   DBNGZD010000207 DBNGZD010000000
VERSION     DBNGZD010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_588626"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..366)
                     /locus_tag="AC16GM_05510"
     tRNA            complement(284..366)
                     /locus_tag="AC16GM_05510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(330..332),aa:Leu,seq:cag)
     gene            complement(906..1580)
                     /locus_tag="AC16GM_05515"
     CDS             complement(906..1580)
                     /locus_tag="AC16GM_05515"
                     /inference="COORDINATES: protein motif:HMM:NF028479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HIR2923170.1"
                     /translation="MDTVITQQGTIFKRFFTSGGDFIPVYTAPALPPSGGFSVCQPTI
                     FAGIAASPFADREYFISDLTRRKVIAFDRLNQPDTLGIATAGGRGVNSPLGLAAQPVG
                     QTWRLGMVEGRDAQVFRNEDTVFTAVYACSTGQELICVGQFQFATAITFDDLGNWFVA
                     DSGANEVAKFDRNGNFVLRFGRTGSGDGEFNGPAGIAFYDRVVYVADSGNSRIVRYVL
                     NTDIRP"
     gene            complement(1737..1991)
                     /locus_tag="AC16GM_05520"
     CDS             complement(1737..1991)
                     /locus_tag="AC16GM_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923171.1"
                     /translation="MRSKQISLATVSLLVTVAGWLALDCARKIQNPVGLPPGLGVPGE
                     TTYVQIAPAWTSAGGVAFSHPHDVIIGQDLFIYVADTDND"
     gene            complement(1991..>4069)
                     /locus_tag="AC16GM_05525"
     CDS             complement(1991..>4069)
                     /locus_tag="AC16GM_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR2923172.1"
                     /translation="SGRVAYRTDKFGFGAPFDFNSDYAVLELSGPEPLSQRLLKNIGL
                     RLPGSITFLVSTNFAFTDGYLGHARSVFTSTLFGGRHLPREDSRFNGLAKVVWKFDPN
                     HKLSYLVQQSITVNQDRSVLETRIRQVTCSYGYPFVYENLLDNYNVFTHWANLEALTW
                     EHKLSPQWSYDLKLSRFFSNLHSDVNGKEWFQYVKPTDNEPYTVTPLPDSTFFVTRGD
                     GFFDQGDGDTWYDHTWENFLLQGSNHLAVKPNWQTQFGFSAAHRRLEVADIFQPYAGA
                     GGLGADLYQTGTGDAALYFDNEINLKGAIVRAGLRYDLWWPGGYVERVLADTTVPFLP
                     DSLRQAFRRQTFSFFGHRAKDVLSPRFGMSNLITGNLSFIFSYNRLAKWPNPQFVYAN
                     LEPRRNSGAYQLFGNPNLNPEKVTLIEAGVKYLIGANDALSIAGYNKSIDDYISAIGV
                     TPDTTNPTQSYLVYFNFDFATSQGVEVEYRKREGDWFEGKVSAAVSRAKGEHALPSDL
                     LRGVNSRAQGRLLEEINFDWDKPWQVNLSANFRALRNRRYRVFGLTLPDNWNGNLFFW
                     AQAGKRYTKFQKAVNPGTGEVSYVQVGKVNDRLGPYWGSFDLTLEKFFLARGFGWSLR
                     LEIQNLFNRKNAVIINPLTGKAYGEGDEIPVGPNFLLQPEPGFRLPIWQVPAQFLAPR
                     SIKLGLAVTW"
BASE COUNT          891 a         1195 c         1155 g          828 t
ORIGIN      
        1 gtctccggag agtttttgaa attgtttgaa ctcttccggc gcggcgacgg ccttgatgcc
       61 gtagtacagt tccttctctt tttgagagag cggcctttga gctaatatgg gggatagaaa
      121 aagtagagat gacgcgactg ccgcgaaagc cacgactttc atttttgttc tcatggcttt
      181 ctcctgtcag ccgaggctaa aagcctcggc tatgggaact acgagctctc tgacccatag
      241 gtcaggcctc cggcctgacc ccaatcaccg gccagaaatt ctatgccgaa ggcgggattc
      301 gaacccgcac gccttgcggg cactgcgccc tgaacacagc gtgtctccca gttccaccac
      361 ttcggcaggc actacttatt caaatatacg tccgcccaaa accaccaaca agcgagcgat
      421 gcacaggtgg ggtgcccgtt tccttttttt ccctccgctg ctcactgacc gtgaattgag
      481 cggttaatta cttaccgtta ccgaaatatg caccggcaac tcgccacccc cggtgcaaca
      541 catttgcact cgggcaccga cacccgataa tcgcagcaga tgctgcatac ttcccacccg
      601 ccgccgccaa cacatcccgg gtacgttgtg caacaaaccc atatcggagg gggcgggttg
      661 ccatcgtccg gcagtgccgt acggctgtac ccgatcccaa ccaggaacgc caggcagaag
      721 gccatcagga aaaccgcaag aagaactttt ttcatcggta gtcacctcct ctgtgttttg
      781 agcaacccca ccctgagaca cgcatcgctt ttacgcctac gtgctatata taaaccggcc
      841 attgagtcat cgcaagcccc gcaaagccga cctgaatgaa tgcgcatcgg gagctgacgg
      901 gtaagtcaag gtcgaatgtc cgtattcaaa acataccgca cgatccgact gttcccggaa
      961 tccgccacgt agaccacgcg gtcgtagaag gcaatgccgg ccgggccgtt gaattccccg
     1021 tcgccggagc cggtgcggcc gaagcggagc acaaagtttc cgttgcggtc gaacttggcc
     1081 acttcattgg cccccgaatc ggcgacgaac cagttgccca aatcgtcaaa agtgatcgcg
     1141 gtggcgaact ggaactggcc gacgcagatc aactcctgac ctgtgctgca ggcgtagacg
     1201 gctgtgaaaa cggtatcctc attccggaac acctgagcgt cccttccttc aaccatcccc
     1261 agccgccagg tttgccccac cggctgggcc gccaggccca aagggctgtt gacgccgcgg
     1321 ccgcccgccg tggcaattcc cagcgtatcc ggctggttca agcggtcgaa tgcaatcacc
     1381 ttgcggcggg tgaggtcgga gatgaaatac tcccggtcgg caaaaggcga ggcggctatc
     1441 ccggcaaaaa tggttggctg acagacgctg aagccgccgg agggcggcag cgccggtgcc
     1501 gtataaacag ggatgaaatc tcctccgctg gtaaaaaatc gcttgaagat cgtcccctgc
     1561 tgggtgatga ccgtgtccac ctgagtggtt tcggttcccg atattcgctg gaaggagcaa
     1621 atcgtgtcgc gctggccgac ggcaagcagg ttgagttgcc gatcctgtgt tatcgccgtc
     1681 gggtgtggta ccgggtagga agcgagaaaa tttcccccga agtcgagttt gacgactcaa
     1741 tcattatccg tatccgccac gtagatgaaa aggtcttgtc cgatgatcac atcgtgcggg
     1801 tggctgaacg ccactccccc ggcggaagtc caggcgggtg caatctggac ataggtggtt
     1861 tcccccggca caccgagacc gggcggcaat cccaccggat tctgaatctt ccgcgcacag
     1921 tcaagtgcaa gccaccccgc caccgtgacg agcaggctca ccgttgccag agagatttgc
     1981 ttcgaccgca tcaccatgtc actgcgagcc ctaatttgat cgaccgcggg gccagaaact
     2041 gtgccggcac ctgccagatg gggagacgga agcccggctc cggctgcaaa aggaaattcg
     2101 ggccgacggg aatttcgtcg ccttccccgt aggccttccc ggtcaaagga ttgatgatca
     2161 ccgcattctt acgattgaaa agattttgaa tttcaagtcg cagggaccag ccgaagccgc
     2221 gcgctaagaa aaacttctcc agcgtcaggt cgaacgagcc ccagtacggc cccagccggt
     2281 cattcacctt gcctacctgt acgtaactca cttccccggt gccggggttg accgcttttt
     2341 gaaacttcgt gtatcgtttc cccgcctgcg cccagaagaa gagattgccg ttccagttgt
     2401 ccggcagggt gaggccgaaa actcggtagc gccggttgcg gagggcacgg aaattggcgg
     2461 aaagattcac ctgccagggc ttatcccagt cgaaattgat ttcctcgagc agccgcccct
     2521 gcgcccggct gttcaccccg cgcagtaggt cggacggcaa agcgtgctcc cctttggcgc
     2581 gcgaaacggc ggcgctcacc tttccctcaa accagtcgcc ttcccgtttg cggtattcca
     2641 cttccactcc ctgggaggtg gcaaaatcga aattgaagta gacaaggtat gactgggtcg
     2701 gattcgtggt gtccggcgta accccgatgg cggaaatgta gtcgtcaatc gacttgttgt
     2761 agccagcaat ggaaagcgcg tcgttggcgc ctatgaggta cttcaccccc gcttcgatca
     2821 gggtcacttt ttcgggattg aggttggggt tgccgaagag ttggtaggcc ccggaattgc
     2881 ggcgcggttc caaattcgcg tagacgaact ggggattggg ccacttcgcc agccggttgt
     2941 aggagaagat gaaggacagg ttccccgtga tcaggttgga catgccgaag cgcggggaga
     3001 gcacatcttt ggcgcggtgc ccgaagaaag aaaaagtctg gcggcggaaa gcttgccgga
     3061 gactgtcggg caggaaaggt acggtcgtat cggcgagcac ccgctcgaca tagccgcccg
     3121 gccaccagag gtcgtagcgc agtcccgccc gcacgatggc ccctttcaaa ttgatttcat
     3181 tgtcaaaata gagcgccgcg tcgcccgtgc cggtctggta gaggtccgcc cccagcccgc
     3241 cggcgcctgc gtacggctgg aaaatatcgg ccacctccag ccgccggtgc gccgcgctga
     3301 aaccaaactg cgtttgccag ttcggcttca cggccaggtg attgctcccc tgcaaaagga
     3361 aattttccca cgtgtgatcg taccaggtgt caccgtctcc ttgatcgaaa aaaccgtccc
     3421 cgcgcgtcac aaaaaatgtg gaatcgggca agggtgtaac ggtgtacggc tcattgtcgg
     3481 tcggtttgac gtactggaac cactctttcc cgttgacgtc ggagtggaga ttggaaaaaa
     3541 atctggagag tttgaggtcg taggaccact gcggcgaaag tttgtgctcc caggtcaagg
     3601 cctccaggtt cgcccagtgc gtgaagacgt tataattgtc caggagattc tcgtagacga
     3661 aggggtaacc gtaggagcag gtcacctgcc ggatgcgcgt ctctaaaacc gaacggtcct
     3721 gatttaccgt aatcgattgc tgcaccaggt acgaaagttt gtgattcgga tcaaatttcc
     3781 agaccacttt ggctaatccg ttgaagcggc tgtcctcccg tggcaggtgc cgcccgccga
     3841 agagcgtact cgtgaaaacg ctccgggcgt ggcccaggta gccgtcagta aatgcgaaat
     3901 tcgtactgac caagaaagtg atagagccgg gaagccgcaa tcctatgttt tttaggagtc
     3961 tctgcgagag cggttccggc cccgaaagtt ccagaacggc gtaatccgaa ttgaagtcaa
     4021 acggtgcacc aaacccgaac ttatcagtcc ggtacgctac ccgacccga
//
LOCUS       DBNGZD010000208         3192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_935383, whole genome shotgun sequence.
ACCESSION   DBNGZD010000208 DBNGZD010000000
VERSION     DBNGZD010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_935383"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1552)
                     /gene="sufB"
                     /locus_tag="AC16GM_05530"
     CDS             complement(137..1552)
                     /gene="sufB"
                     /locus_tag="AC16GM_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR2923173.1"
                     /translation="MATDQLLDIGQQYETKYGFHDPEKYVFKAKPGLSQQVVEGISSM
                     KGEPEWMRKFRLRALDIFLKKPMPTWGNTELLNSIQFDTIHYYLKPMEEQGRSWDEVP
                     ADIKRTFDRLGIPEAERKFLAGVSAQYESEVVYHSIREDLSKQGVVFLDMDSGLREYP
                     DLVKRYFGTIIPAGDNKFSALNSAVWSGGSFVYVPPGVHVRVPLQAYFRINAEAMGQF
                     ERTLIIVDEGASVHYIEGCTAPVYSRDSLHSAVVELVALKGAKLRYTTIQNWSKNVYN
                     LVTKRAVAYEDATVEWVDGNLGSKLTMKYPSVYLMGKGAHGEVLSIAFAGRDQHQDAG
                     AKMVHVAPNTSSTITSKSISKDSGRTSYRGLVKVYRNAKGVKSSVRCDALLLDEESRS
                     DTYPTMEIDEDQVQVGHEATVSKVGEEQLFYLTSRGLTEAEATLMIINGFIEPFTKEL
                     PLEYAVELNRLIQLEMEGSVG"
     gene            complement(1623..2396)
                     /gene="sufC"
                     /locus_tag="AC16GM_05535"
     CDS             complement(1623..2396)
                     /gene="sufC"
                     /locus_tag="AC16GM_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR2923174.1"
                     /translation="MSQNRPVLVIQDLHVGIEGKEILKGVDLRINKGEVHALMGPNGS
                     GKSTLSNALMGHPKYQVTRGQVLFKGENIIEMKADERARKGIFLAFQYPTPIPGVTVT
                     NFMRSSLKAVKGKEIPPREFLKLLREKMKTLEIDEEFAHRYVNDGFSGGEKKRHEILQ
                     MAMLEPEMAILDETDSGLDIDALRIVAKGVNQLIGPNLGVLIITHYQRILNYITPDFV
                     HVMADGQIVRSGDRKLAHDLEEKGYDWLLPETQKEAVPA"
     gene            complement(2482..2958)
                     /locus_tag="AC16GM_05540"
     CDS             complement(2482..2958)
                     /locus_tag="AC16GM_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR2923175.1"
                     /translation="MRITKQEEYGLRCILQLAQNNGDAPLTIPEIASREGLSIDYATK
                     LLILLRRSGLVQSVRGVKGGYRLAKRSENTSVREVLSALGWSFLDEDLCSHYPGKMQE
                     CVHLGGCGIRPIWSTVARQIYALLDKTTLAQLLQEEQMVSHQLEARVQAKTGIERG"
BASE COUNT          624 a          920 c          857 g          791 t
ORIGIN      
        1 ctggcgcggt ttgggtcgtt tctttgaaca ttagctaatc tttcgtcctt caaaaacagg
       61 cgcggccagg gggccgcgcc tacgcgtcgt aatttttatg cgcgtagggc gagcgccccg
      121 ctcgcccgct ctaaacttat cccaccgagc cttccatctc caactggatc agccggttca
      181 actccactgc gtactccaac ggcagttcct tggtgaacgg ctcgatgaag ccgttaatga
      241 tcatcaaagt cgcttccgcc tcggtcaagc cccggctggt gaggtagaag agctgctctt
      301 cgcccacttt ggaaacggtg gcctcgtggc cgacctgcac ctggtcttcg tcgatttcca
      361 tcgtcgggta ggtatccgac cgcgactcct cgtccagcaa aagcgcgtcg cagcggacgc
      421 tggacttgac gcctttggcg ttgcggtaga cctttaccag cccgcggtag gaagtgcggc
      481 ccgaatcttt ggaaatcgac ttcgaagtaa tcgtcgaact ggtattgggc gccacatgca
      541 ccatcttggc gccggcatcc tggtgctggt cacggccggc aaaagcgatg gagagcacct
      601 cgccgtgcgc gcctttgccc atcaagtaga ccgacgggta cttcatcgtc agtttggagc
      661 cgaggttgcc gtccacccat tcgacggtgg cgtcttcgta ggccacggcc cgcttggtca
      721 ccagattgta gacgttcttc gaccagttct ggatcgtcgt atagcgcagt ttggcgccct
      781 tgagcgccac taattccacc accgcggagt ggagcgaatc gcgcgagtag accggcgcgg
      841 tgcagccctc gatgtagtgg acgctcgcgc cttcatcaac gatgattaac gtccgttcga
      901 actggcccat cgcctcggca ttgatgcgga agtaggcttg caggggaact ctgacgtgca
      961 cgcccggcgg cacgtagacg aacgaaccgc cgctccagac agccgagttc aacgccgaga
     1021 atttattgtc gccggccgga atgatggtgc cgaagtagcg cttgaccaag tccgggtact
     1081 cgcgcaggcc ggaatccata tccaggaaaa cgacgccttg cttggacagg tcctcccgta
     1141 tggagtggta gaccacttcc gactcgtact gggccgagac gccggccaaa aacttgcgct
     1201 ccgcttccgg aatgcccaaa cggtcaaagg tccttttgat gtcggccggc acctcgtccc
     1261 acgagcggcc ctgctcttcc atcggcttga ggtagtagtg gatggtgtcg aactggatgg
     1321 agttcagaag ttcggtattg ccccaggtcg gcattggctt cttgaggaag atgtctaagg
     1381 cccggaggcg gaatttccgc atccactccg gctcgccttt catgctggag atcccttcca
     1441 ccacctgttg tgaaaggccc ggtttggctt tgaagacgta cttttccggg tcgtggaaac
     1501 cgtacttggt ttcgtactgc tggccgatat ctaagagttg atcagttgcc atctttgctt
     1561 acctcctcag gaggttcttt tgacctcacc cttaccctct cctacaggag agggaataaa
     1621 agctatgccg gtaccgcctc tttttgggtt tccggcaaaa gccagtcgta ccctttttct
     1681 tccaagtcgt gcgccaattt tctgtcgccc gagcgcacga tctggccgtc ggccatcacg
     1741 tggacgaaat cgggcgtgat gtaattcaaa attctctggt agtgggtgat gatcaggacg
     1801 cccagattcg gcccgattaa ctggttgacg cctttggcca cgatgcggag cgcatcaata
     1861 tccaacccgg aatcggtttc gtccaagatt gccatttccg gttcgagcat tgccatctgc
     1921 agaatctcgt ggcgtttctt ttcgccgccg gaaaagccgt cgttcacata gcggtgggcg
     1981 aattcttcgt cgatttcgag cgtcttcatc ttctcgcgca atagtttcaa aaactcccgc
     2041 ggggggattt ctttgccttt gaccgccttc aacgaactcc gcatgaagtt ggtcaccgtc
     2101 acacccggaa tcggagtcgg gtactggaag gcgaggaaga tccctttgcg cgcccgctcg
     2161 tcggctttca tttcaatgat attctcacct ttgaagagca cctggccccg ggtgacctga
     2221 tatttcggat gacccatcag ggcgttggag agggtgcttt tgccggagcc gttggggccc
     2281 atcaaggcgt gcacctcgcc tttgttgatg cggagatcga cacccttcaa aatctctttg
     2341 ccttcaatgc cgacgtgcaa atcctgtatg acaagcaccg gtctgttttg actcattggc
     2401 caattttcta ctgtttgcga aattcttcac aagccatact atcgatacct gaaagttttg
     2461 tcaagtttcg tcccaaaaaa attaccctct ctctattccc gtctttgctt gcacccgtgc
     2521 ttccaactgg tgagacacca tctgctcttc ctggagcagt tgggccaggg tcgttttgtc
     2581 cagcagggcg tagatttgtc tcgccaccgt ggaccagatg gggcggatgc cgcagccgcc
     2641 gaggtggacg cactcctgca ttttccccgg atagtggctg cagaggtctt catccagaaa
     2701 cgaccagccc agggcgctca agacctcccg gacgctcgta ttttctgacc gcttagccag
     2761 ccggtagccg cctttaactc cccggacgct ctgcaccaag ccgctccgcc ggaggaggat
     2821 caaaagtttg gtggcatagt cgatagacag cccctcccgg gaggcgattt ccgggatggt
     2881 caaaggcgca tccccgttat tctgagctaa ctggaggatg caccggaggc cgtattcttc
     2941 ctgcttagtt attctcatat ctccccttgg caggggtgag cgaagtcgaa cccttgtttg
     3001 gttattcgca agctatctcc ttatccacac tatacatacg aaatcctgac gagtttgtta
     3061 ggattcctat aaagaaatat cggcagggaa gtcaggtttg tcaaggggaa ttttggggct
     3121 atcttgatct ctcttggtga ccattcaaac cccttctacg ggtatccttt ttctaataac
     3181 aattttgctt ga
//
LOCUS       DBNGZD010000209         5330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_449244, whole genome shotgun sequence.
ACCESSION   DBNGZD010000209 DBNGZD010000000
VERSION     DBNGZD010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_449244"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC16GM_05545"
     CDS             complement(<1..244)
                     /locus_tag="AC16GM_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923176.1"
                     /translation="MSHKIIVAVLGMLVVIDWFASGELAQAADPTTVVALPAMENPLG
                     VADYFAGQSLVVYQNAAVFDSDQPPLPGTKDVYAVQS"
     gene            complement(259..3516)
                     /locus_tag="AC16GM_05550"
     CDS             complement(259..3516)
                     /locus_tag="AC16GM_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923177.1"
                     /translation="MRKRKIVKTVGPIAVLLAAGAGAGGLAFPHLLAAQTLSVQEIVR
                     PEPTDKVTAETRALFKSSVSDKNLLHLERETPFGLPKLTGRATQVDTVHVLAIRVEFQ
                     FENPDDSTTTGRGLFDRSAPGQSLIDPPPHGRLYFQAHLRALANYWRAVSNGKLEIVG
                     DVFPTAELQAYQLPVTMAYYGSKGPWKDSSITAQLGHFFYDAVLLADTQEPAIDFSRY
                     DALILFHAGADRQNDLGDLVAYTPNDLFTGFIVLDTSLSPRVDNDSVRITGGIIAPES
                     PSQDNRVVALNGVLAHEFGHVLGLPDLYNTTSRPPITQLGDFSLMDNFGQDVGVVASD
                     GIRTVTAFGLVPTYLDAWSRAFLGFLDTLAEIRNGQNLQLAAAALQQTGIEAVKVPIS
                     SREYFLLENRQEDIDGITTNIIVDSVTNVVLGTGYRPPGQPRQFSDEYDILLPGSGIA
                     VYHVDEGVAYLLDPDFPQFTKFETNSVQTDPNRRMVALEEADGYVDFGGNYFTGFGTS
                     TDLYRAGNNTAFTPTSNPSTRTNFKANSHISITNISASSPFMTLDVKTDFTAPGWPVW
                     AGKDTGTVPVVIDTTAGRPLIFTRHGQYVLGWEKTGQPIFSNAFSDTVRNLNNSTSVH
                     PLAVFARAAAPLSAGPSVADINGDGAVDVVAVGTDRKLYVWTNRDANIDGLADSLPGF
                     PVSLVDTVLLAPVCLNDSTIPFLPNARILVGGKNERFDAFSAGGTSVAVGSLGAAIEG
                     ISVDETGKIYLQLRRPEGVTLTEFPNIWLRMITSGTQATAGVWGPVTGDLNRDGVPDI
                     VLVDQSGLVHAFDSAGVYLPGFPVRFSQPFVAEPILADFDGDGYLDIVGVTQNKIAVF
                     NRAGVMVTDFPVGPEVHDLAGYLPQPPVFLPVDNSNVGMFLASTRNGNLNAFHQTNLA
                     PATFPLSIGSQARSTIAAVERLGRTQLFLKGSDGFLYSFLLDAGSQPLATAWPQFGYN
                     AGHTHSFPSNLLPPVARPAGPLVRKESFFPYPNPAQRATKLRYYLTEDADVTFSFFDL
                     AGNLVEEKRVRGGGSRDNDFDWDCSTMAAGVYLCRMEAKSAGQKQVEFTKIAVVR"
     gene            complement(3542..>5330)
                     /locus_tag="AC16GM_05555"
     CDS             complement(3542..>5330)
                     /locus_tag="AC16GM_05555"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2923178.1"
                     /translation="PVTALTGEESNLWVGTESGLYRFDGSAWRRFGRADSLASEKITC
                     LAAGPNKTIWIGTDAGVLRTDGRQVTNYDTTSGLPGQPIVALAVKGERLAYAATQDDL
                     YRLDGERWSDALAYTLRVNDTWERVAAGFTGRQDDRKVAAVVKALKEVNHAGDTATVA
                     GAVVRLPYRVALESPITTLEIDKEDHLWIGTAAGLKRFAEGEFTVWGYDWHAVKEGET
                     VQSIAAATLGSTDSAKVALLVEKIKAYNQLGDEPLAAGQKIGVYDNILGSPTRSIGTN
                     GDKILVGTAFGTLMYRNEKWSRYYHNGLDKVGAAAIFAVDGELFFGALNRVVVYAHAR
                     REITFMHVNWLPELATDLAYEYLSYVQHVEGWGTLGGNVTFLNLGRNVRTSETGDTLG
                     VFDSFDAALTLSYGTRLTRSLVGGISAKVIYSNLANVGAGAELGSGRGSSVALDFGLL
                     YQTPFPRLNLGLAVTNLGPNITYIDANQSDPLPRNLAFGWAYKVFDTPYNRLTFVGEV
                     NKELVDLNSPRSVELKEAIVNAGLEYWYGSFFALRTGYIYDQLGDIKTPTFGGGLQYS
                     SFRIDFSYIPASRDLALANTLRLSVTGRF"
BASE COUNT         1122 a         1556 c         1613 g         1039 t
ORIGIN      
        1 tgctctggac cgcgtagacg tccttcgtcc cgggcaaagg cggctgatcg gaatcgaaaa
       61 ctgccgcatt ctggtaaacc acaagcgact gcccggcaaa gtagtccgcc accccaagcg
      121 ggttctccat cgccgggagc gcgacgacgg tcgtcgggtc agctgcttga gctaattcgc
      181 cagaagcgaa ccagtcaatt accacaagca tccccaacac ggccactatg attttgtgtg
      241 acataagaac ttcctctctc atcgcaccac ggcgattttg gtaaattcca cctgcttctg
      301 gccggcgctt ttcgcttcca tccggcagag gtacacgccg gcggccatcg tactgcaatc
      361 ccagtcgaaa tcgttgtccc ggctgccgcc gccgcgcacc cttttctcct ctaccaaatt
      421 acctgccagg tcaaagaagg aaaaggttac gtcggcatct tccgtcaaat agtaacgcaa
      481 cttcgtcgcc ctttgtgccg ggttggggta agggaaaaat gactctttcc gcaccagtgg
      541 ccccgctggc cgggccactg gcggcaaaag gttcgagggg aatgagtggg tgtgtccggc
      601 attgtagccg aactggggcc aggcagtggc cagcggctgt gagccggcat ccaggaggaa
      661 actgtagagg aagccgtctg aacccttgag gaacagttga gtccgaccta aacgctccac
      721 cgcggcgata gtcgagcgcg cctgagagcc gatagaaagc ggaaacgtcg cgggagccag
      781 attggtttga tggaaggcat tgagattgcc attgcgggtc gaggccaaaa acatgccgac
      841 gttagaatta tccaccggca gaaaaaccgg cggctgcgga aggtaaccgg ccagatcgtg
      901 cacctcgggg ccgaccggaa aatcggtcac catcaccccg gcccggttga agaccgcaat
      961 tttattctgg gtgacgccga caatatccag atatccgtct ccgtcgaaat ccgccagaat
     1021 cggttcggca acgaatggtt gtgaaaaccg caccggaaat cccggcaagt agacgccagc
     1081 cgaatcgaag gcgtgaacta ggccgctctg atcaacgaga acgatgtccg gaacgccgtc
     1141 gcggttgaga tcgcccgtca ctggtcccca gacgcccgcg gtggcctgag taccgctcgt
     1201 aatcattctc aaccaaatat tggggaattc cgtgagggtt actccctcgg ggcgcctcaa
     1261 ctgcaggtag attttgccag tttcatcaac gcttatgcct tcgatggcag ctccaaggga
     1321 gccgacagcg actgatgtac caccggcgga aaaagcatcg aaccgttcgt tctttccgcc
     1381 caccagaatt cgtgcgttcg gcagaaacgg aatagtcgag tcattcaggc aaaccggagc
     1441 cagaagcacg gtgtccacca acgacaccgg aaaaccaggc agagaatcgg ctaaaccatc
     1501 tatgttcgca tcgcgatttg tccaaacata tagcttacga tcagttccta cggctacgac
     1561 atctaccgcg ccgtcaccgt tgatgtccgc caccgacggc ccggcggaaa gcggagcggc
     1621 tgctcgtgca aagaccgcca gcgggtgaac tgaggtagag ttattgaggt tccggaccgt
     1681 atcggaaaaa gcatttgaga aaattggctg gcccgttttc tcccatccga gcacgtactg
     1741 gccgtggcgg gtgaaaatga gcgggcggcc tgcggtcgtg tcgatgacga ccggaactgt
     1801 gcccgtatcc ttccccgccc agaccggcca gccgggcgcc gtgaagtcgg tcttcacgtc
     1861 taaagtcata aaggggctgc tggcgctgat gttggtaatg gaaatgtggc tgttggcttt
     1921 gaagttggtg cgggtggaag gatttgacgt gggagtgaag gcagtattgt tccccgcgcg
     1981 ataaaggtcg gtggacgtgc cgaagccggt gaagtagttg ccgccaaaat cgacatagcc
     2041 gtcggcttct tccaaggcca ccatccggcg gttcgggtcg gtctggactg aattcgtctc
     2101 aaacttggtg aactggggaa agtccggatc aaggaggtag gccacgccct catcgacgtg
     2161 ataaacggcg atgcctgagc cggggagcaa gatatcatat tcgtctgaga attgccgcgg
     2221 ctgccccggc gggcggtagc cggtgccgag caccacgttc gtgaccgaat cgacgatgat
     2281 gttcgtggtg atgccgtcaa tgtcctcctg ccggttttcc agaaggaaat actcgcgcga
     2341 actgatcgga actttgaccg cctcgatccc ggtctgctgc agggcggcgg cggccaattg
     2401 gagattctgc ccgttgcgaa tctccgccaa agtatcgagg aagcccagaa acgcccgcga
     2461 ccaggcatcg aggtaggtag gcaccaagcc gaatgcggtg accgtccgga tgccgtctga
     2521 ggccaccact cccacgtcct gcccgaaatt gtccatcaga gaaaaatcgc ctaactgggt
     2581 tatgggtgga cgtgacgtag tgttatacag gtcgggcagg ccgagaacgt ggccgaactc
     2641 gtgcgccaaa acgccgttca gcgccaccac ccgattatcc tgcgagggcg actccggcgc
     2701 aataatgcct ccggtgattc ttacactgtc gttatcgaca cgaggagaaa gcgaggtgtc
     2761 cagaacgata aagccggtaa agagatcatt cggggtataa gcgacgaggt cgcccagatc
     2821 attttgccgg tcagccccgg cgtgaaagag gatcagagca tcatagcggg agaaatcaat
     2881 ggccggttcc tgcgtatcag cgagaagcac cgcgtcgtaa aagaaatggc ccaattgggc
     2941 ggttatggaa gaatccttcc acggcccctt agagccgtag taggccatcg tcaccggtaa
     3001 ctggtaagcc tgcagttcag cggtagggaa cacgtccccg acgatttcca actttccgtt
     3061 ggaaaccgcc cgccagtagt tcgccagtgc ccgcaggtgg gcctggaagt acagtcggcc
     3121 gtgcggcggc gggtcaatga gggactgacc gggagcgctg cggtcaaaaa ggccccggcc
     3181 cgttgtagtg gaatcgtccg ggttttcaaa ttgaaactcc acgcggatgg ccagcacgtg
     3241 caccgtgtcc acctgagtgg cgcgcccggt cagtttgggc aggccgaaag gcgtttcgcg
     3301 ctccagatgc aggaggtttt tgtcggacac gctacttttg aaaagcgccc gggtttccgc
     3361 agtgacttta tcggttggct ctggtcgcac tatttcctgg acggaaagag tttgggccgc
     3421 cagcaggtgg gggaaagcca gaccccctgc gcccgcaccg gcagcaagta gtactgctat
     3481 cgggccaacg gtctttacaa tcttccgctt tctcatattc caacggcctc actacggcca
     3541 cttagaacct tccggtcacc gagagccgca gggtgttggc gagggccagg tcgcgggaag
     3601 ccgggatgta ggagaagtcg atccggaaac tcgaatactg caacccgccg ccgaaggtgg
     3661 gcgttttgat gtcgcccagt tggtcgtaga tgtagcccgt gcgcagggcg aagaacgagc
     3721 cgtaccagta ctccagcccg gcgttgacaa tcgcctcttt gagctcaacc gagcgcgggg
     3781 agttcaggtc aaccagttcc ttattgacct ctcccacgaa ggtcagccgg ttgtacggcg
     3841 tgtcgaagac cttgtaggcc cacccgaacg ccaggttccg cggcagtggg tcggactggt
     3901 tggcgtcgat gtaggtgatg ttcgggccta agttggtgac cgccaagcct aagttgagcc
     3961 ggggaaaggg ggtctggtag agaagcccga aatcgagcgc taccgacgag ccgcggccgg
     4021 agcccagttc cgcaccggcg cccacgttgg ccagattgct gtaaatgacc ttggccgaaa
     4081 tgccgcccac cagagaccgc gtcagccggg tgccgtagga gagggttaaa gcggcatcga
     4141 agctgtcgaa cacccccagc gtatcgccgg tctcgctcgt ccgcacgttg cggcctaagt
     4201 tgagaaaggt gacgttgccg cccagagtgc cccagccttc gacgtgctgg acgtaggaga
     4261 ggtattcgta ggccaggtcg gtggccagtt ccggcagcca gttgacgtgc atgaaggtaa
     4321 tctcccgccg ggcgtgcgcg tacaccacca cccggtttag ggcgccgaag aacagttcgc
     4381 catcgacggc aaagattgcc gccgcaccaa ctttgtccag cccgttgtgg tagtagcggc
     4441 tccatttttc gttcctgtac attaaagtcc cgaaggcggt gcccaccaga attttgtcgc
     4501 cgttggtgcc gatggagcgg gtggggctgc ccaggatgtt gtcgtacacg ccgattttct
     4561 ggcccgcggc cagcggttcg tcacccagct ggttgtaggc tttgatcttc tccaccaaaa
     4621 gggccacctt ggctgagtcg gtagagccga gagtcgcggc ggctatcgat tgcacggttt
     4681 caccctcttt gaccgcatgc cagtcgtagc cccagacggt gaattcccct tcggcaaagc
     4741 gcttaaggcc ggccgcggtc ccgatccaga gatggtcttc cttgtcaatt tccagtgtcg
     4801 tgattggact ttccaaagcc acccgatagg gcagccgcac cacggccccc gccacggtgg
     4861 cggtatcgcc ggcgtggttg acttccttca gcgccttgac caccgccgcc accttccggt
     4921 cgtcctgacg cccggtgaat ccagccgcca cccgctccca ggtatcgttt acccgcaagg
     4981 tgtaggcaag ggcatcggac cagcgctctc cgtcgagccg gtacaggtca tcttgggtgg
     5041 cggcgtaggc cagccgttca cccttgactg ccagagccac aatcggctga ccgggcaggc
     5101 cggaagtggt gtcgtaattg gtcacctggc ggccatcggt gcgcaagacg cccgcgtcgg
     5161 tgccaatcca gatcgtcttg ttcggaccgg ccgccaagca ggtaattttt tcgctggcca
     5221 gactgtcggc ccggccgaag cgccgccagg cgctgccgtc aaaacggtac aaaccggatt
     5281 cggtgccgac ccataaatta gattcctcac cggttaaagc cgtcaccggt
//
LOCUS       DBNGZD010000210         5420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1561092, whole genome shotgun sequence.
ACCESSION   DBNGZD010000210 DBNGZD010000000
VERSION     DBNGZD010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1561092"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1793
                     /locus_tag="AC16GM_05560"
     CDS             93..1793
                     /locus_tag="AC16GM_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2923179.1"
                     /translation="MLANQSVPAVDSDAAMNVGDPIPELRFPGDPACVPACILIVDDE
                     ERMCASLAALLSRAGYNVEATAEAEGALQWLKTRTYDLVIADIKMPGVDGLELLRVAR
                     QADPDALVILMTGYGSLDSAVAAIGSGAYDYLLKPIEFPALELVVKRAFEKRRADLSR
                     RALLAELQAKNELLSRKFAQLDALYQAARSLAVARDLDELLVKIVTLATEVIGARVGS
                     IMLVEPETQGLVVKAALGLEPEIVRTTRLPFGSSIAGYVAQRSEPVVVADVETDSRFA
                     RVAKAKYETKSLLCAPLVVKQEVLGVINLADKRGGGVFGPDDLQLLVTFASQVAVAID
                     DARQIEQALQNARRLVTMGSLLSEISHDLKAPLTNIRGSLQILREKLARSPEAAEILK
                     ATEGEVYRLTELVKELVDFSNPNKYHLQRADLSGVIRRALKLVESDLAKKKIDLRLRM
                     TADLPPIFLNENELIEMLLNLITNAFEGMPDGGILEVGAQGEQGDVRVWVKDSGAGIS
                     PERLPRIFERYYTTKPTGTGLGLPIVERIVKAHNGTITVESTPGKGTTFAVTFPGAER
                     "
     gene            1860..3254
                     /locus_tag="AC16GM_05565"
     CDS             1860..3254
                     /locus_tag="AC16GM_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2923180.1"
                     /translation="MRSTDKVKILVIDDDPKVPWILREGLDRSFELLEARDGFEGIQQ
                     VSKYKPQLVLLDLKMPGLTGLEVLEKIKTAEPATDVIMLSGHGDTQNIVESMRLGAAE
                     FINKPFDVKEVEIHVQSVLEKSRLKREVHHLRTELSTRAEYEHFVGDSPTMLEVKNII
                     EQVADSDLTVLIRGESGTGKEIVARMLHTLSSRRNEPFNKVNCAAIPRDLLEAELFGY
                     EKGAFTGAHKNKPGRIEVANKGTVFLDEIGDLPLELQSKLLQVLEQHEFVRVGGIHTI
                     KVDVRIVCATNRDLEKAIQGGQMRDDLFYRLNEITIPLPPLRERREDVPLLIDHFLKK
                     YNTQYHKEVASLSARAMTLMQQFPWPGNVRQLENVIKQVVVRGDEQIIGDLLRDRGKA
                     VPEAALPLPSGPNTYSLKKRVEALVTQEEKRLIAEVLHKTNWNRRKAADLLAISYRSL
                     LYKIKEYRINEVAE"
     gene            3582..4064
                     /locus_tag="AC16GM_05570"
     CDS             3582..4064
                     /locus_tag="AC16GM_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl cyclase domain-containing protein"
                     /protein_id="HIR2923181.1"
                     /translation="MTARSFDSVPYEEPDEAGLHRSHRSFQTRARQELARAERYAAYL
                     SLVTLDLSCLLRSLAPDQKNEEMNRLFDELYDLVRTRVRTSDVVSGVENARLGLLLIE
                     TAKSGAQTLAARLAEQVRAYLASHVPLPSDWQVPVEIDSFPDVHSGREQFLSRLGTFQ
                     "
     gene            complement(4197..4274)
                     /locus_tag="AC16GM_05575"
     tRNA            complement(4197..4274)
                     /locus_tag="AC16GM_05575"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4236..4238),aa:Glu,seq:ttc)
     gene            4444..>5420
                     /gene="aroC"
                     /locus_tag="AC16GM_05580"
     CDS             4444..>5420
                     /gene="aroC"
                     /locus_tag="AC16GM_05580"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR2923182.1"
                     /translation="MLRFLTAGESHGQALAGILEGMPAGLGVDISFLNAELARRQKGY
                     GRGGRMQIEKDTLRILSGVRHLKTLGSPIAFLIENRDWENWKEIMAVEDNPDSAAVAD
                     PRSAPRTAPRPGHADLAGAIKYGQKDIRNVLERASARETAARVGAGAFAKLFLREFGI
                     DFASHVVEICGVAVPDGRPAFAEIQSRAPKSELHCIDPATEEKMKAKIDEAIARKDTV
                     GGVFEVIVTGVPIGLGSFVHWDRRLDAQLAAAVMSIPSVKGVEIGMGFAQAHRFGSEV
                     HDQIFFEGKTQSASRQKGFFRRTNNAGGLEGGVTNGEDVVLRAVCKPLST"
BASE COUNT         1129 a         1556 c         1694 g         1041 t
ORIGIN      
        1 caccacggca ccatccgggc caaaaatagg gtaccacatg gtgcctgctt cactctggaa
       61 tttcccgtgg cccaggagta gccggctggg tcatgttggc gaatcagtcg gtaccggcgg
      121 tagactctga cgcggcaatg aacgtgggtg acccgattcc cgagcttcgc ttccccggtg
      181 accctgcctg cgtgccggcc tgtatcctga tcgtcgacga tgaggagcgg atgtgtgcca
      241 gtctggcggc gctcctgagc agggccggct acaatgtgga ggcaaccgcc gaggcagaag
      301 gcgccctcca gtggctcaaa acgcgcacct acgacctggt gatcgccgac atcaaaatgc
      361 cgggggtgga cggattggaa ctcctgcggg tcgcccggca ggccgatccg gacgccttgg
      421 tcatcctcat gaccggctac ggatcgctcg attcggccgt ggccgccatc gggagcggtg
      481 cttatgacta cctcttgaaa ccgatcgagt ttcccgcatt ggagttggtg gtcaagcggg
      541 ctttcgaaaa gcgccgcgcc gacctgtcgc gccgggccct tttagccgag ctccaggcga
      601 aaaacgaact gctctcccgt aagttcgccc agctggatgc cctctaccag gcggcccggt
      661 cgctggcggt ggcccgcgat ttagacgagc tgttggtcaa aatcgtgacg ctcgcgacgg
      721 aggtgatcgg tgcccgggtg ggttcgataa tgctggtgga gccggagacg caggggttgg
      781 tcgtcaaagc cgccctgggg ctggaacccg aaatcgtccg cactacccgg ctccctttcg
      841 gctcttccat cgccggctac gtggcgcaaa ggagcgaacc ggtggtagtg gccgacgtgg
      901 aaaccgacag ccgctttgcc cgcgtggcca aagcgaagta tgaaaccaaa tcgcttttgt
      961 gcgctcctct ggttgtcaaa caagaagtgt tgggcgtgat caacctcgcg gataagcggg
     1021 ggggcggggt cttcggtccg gacgacctgc agctgctggt caccttcgcg tcgcaggtgg
     1081 cggtggctat cgacgatgcc cgccagatcg agcaggcgct ccagaacgcc cggcgcttgg
     1141 tcaccatggg gagtcttttg tcggagatct cccatgacct gaaagcgccc ttgaccaata
     1201 tccgcggcag cctccagatt ctccgggaga agctggcgcg ttcccccgaa gccgccgaga
     1261 tcctgaaggc gacggaaggc gaagtctatc gcttgaccga actggtcaag gaactggtgg
     1321 acttctccaa tcccaacaag taccacctcc agcgggccga cctctccggc gtcatccgcc
     1381 gcgccttaaa gctcgtggaa agtgacctgg ccaaaaagaa aatcgatctc cggctgcgga
     1441 tgaccgccga tctgccgccg attttcttaa acgaaaatga gcttatcgag atgctcctga
     1501 acctgatcac caacgcgttc gagggcatgc ccgacggcgg cattctggaa gtcggcgcgc
     1561 agggagagca gggtgacgtg cgcgtgtggg tcaaagacag cggggcgggc atcagtccgg
     1621 agcggttgcc ccggattttt gagcgctact ataccaccaa gccgaccggc accggtctgg
     1681 gactgccgat agtcgaaagg atcgttaaag cccataacgg taccattacc gtcgaaagta
     1741 cgccggggaa ggggacgact tttgcggtca ccttccccgg cgccgagcgt tagccacgtg
     1801 gggaggcagg ccggcatgcc gatagcaata aggtagccgc ggctgtgcgg acaaacgcga
     1861 tgcgaagcac cgataaggtc aaaatactgg tcatcgacga cgatcccaaa gtcccctgga
     1921 ttttgcggga agggctggac cggtcgtttg aacttttgga agcccgggac ggttttgaag
     1981 ggattcagca ggtttccaag tacaagcccc agttagtgct cttagacctc aagatgccgg
     2041 ggttgaccgg cctggaagtc ctggaaaaaa tcaagaccgc cgaaccggcc accgacgtca
     2101 tcatgctctc cggccacggc gacacccaga acatcgtcga atcgatgcgg ctgggcgcag
     2161 cggagttcat caacaagccc ttcgacgtca aggaagtgga aatccacgtc cagagcgtcc
     2221 tggagaagag ccggctcaag cgcgaggtgc accacctgcg caccgaactt tcgacccggg
     2281 ccgagtacga acacttcgtg ggcgacagcc cgaccatgct ggaggtgaaa aacatcatcg
     2341 agcaggtggc cgacagcgac ctcacggttc tgatccgggg ggaaagcggc acggggaaag
     2401 aaatcgtcgc ccggatgctg cacacgctgt cgtcccggcg caacgagccc ttcaacaagg
     2461 tcaactgcgc cgccattccc cgcgacctcc tggaggccga actgttcggc tacgagaaag
     2521 gtgctttcac cggcgcccac aagaataaac cgggccggat cgaagtcgcc aataagggta
     2581 ctgtcttctt agacgaaatc ggcgacctgc ccctggaact ccagtcgaaa ctgctccagg
     2641 tgttggagca gcacgagttc gtccgggtcg gcggtattca caccatcaag gtggacgtcc
     2701 ggatcgtctg cgccaccaac cgcgacctgg agaaagcgat tcagggtggg caaatgcgcg
     2761 acgacctgtt ctaccgcctg aatgaaatca ccattccctt gccgcccttg cgcgagcggc
     2821 gggaagacgt cccgcttttg atcgaccatt tcctgaagaa gtacaacacc cagtaccaca
     2881 aagaggtggc gtcgctttcc gcccgggcca tgaccctgat gcagcagttc ccctggccgg
     2941 gcaacgtccg gcagctggaa aacgtcatca agcaggtggt ggtgcgcggc gacgaacaaa
     3001 ttatcggaga tttgttgcgc gaccgcggca aggcggtgcc agaggctgcc ctcccccttc
     3061 cttccggtcc gaacacttac tcgctcaaaa agagagttga agctctggtg acgcaggaag
     3121 aaaaacgttt gattgccgaa gtgctacaca agacgaattg gaaccgccgg aaagccgccg
     3181 atcttttggc catcagctac cgctcgctct tgtacaaaat aaaagagtac cgcatcaacg
     3241 aggtggccga atagtcgggg atgtagaaaa actgaacggt tttgcaactc ctggtcgagc
     3301 aggtgcagag tggcaatcgc ggagtgggac agaaagaaaa gttacggtgt tccgctacct
     3361 cgttggggca aaggccagga gcgcttttgc ccggaccgat ttaaccgcgg agcgtttgac
     3421 cgggggtaac ttttactgtg gcattgagtt tgctgtaaaa aagtcgcagc agagcctgga
     3481 tgcggatcaa agggtgaggg tgggtgtccc aaaagatttc ggtaggataa gcggtgggag
     3541 agaaattctc tatcactgtc aacccaggga aggggagcag catgaccgcc agaagcttcg
     3601 actcggtacc gtacgaagaa cctgacgagg cgggactgca caggtcccac cgatctttcc
     3661 agacgcgcgc caggcaggag ctggcccggg ccgaacggta cgcggcctac ctttctctgg
     3721 tcaccctcga tttaagttgt cttttgcgct cgctcgcgcc ggatcaaaaa aatgaggaaa
     3781 tgaaccggct gtttgacgag ttatacgacc tggttcgtac ccgggtgcgg acgagcgacg
     3841 tggtctcggg ggtggagaac gcccgtctcg gtttgctttt gattgaaacc gccaaaagcg
     3901 gtgcgcagac cttggcggcc cgcctggcgg aacaggtccg cgcctatctg gccagtcacg
     3961 tccccctgcc ctccgactgg caggtgccgg tggagattga ctcgtttccc gacgtgcact
     4021 cgggccggga gcagttcctc tcccggctgg ggactttcca gtaagttgtg tgagtacgtc
     4081 agccggtcgt tgagtgcggc cggctaccca tttcttcctg aactacattc gcagggaagc
     4141 ggtgtcattt ttccccgtct ttttcaccga tccctgatct ctgatccttt tttttgtggt
     4201 gtccccaacg agatttgaac tcgtgttacc gccgtgaaag ggcggtgtcc tagaccggcc
     4261 tagacgatgg ggacggtggg taattgcccg ccttgggcgg ctgcgggaag atacgttggc
     4321 cgcgcagttt ccgtcaagac gggccgttga tcttcccccg aacgtaattt ggaattgtcg
     4381 cgccaccgtc gtattttctc tttcacttcc ggaaactcat ttgggttgtt cggggtaaag
     4441 acgatgttgc gctttttgac ggcaggagaa tcacacgggc aggccttagc gggcatcctc
     4501 gaggggatgc cggcgggcct gggggtcgat atttctttcc ttaatgctga actggcgcgg
     4561 cggcagaagg gctacggccg gggcggacgg atgcaaatcg aaaaggatac gctccggatt
     4621 ctctccggcg tccgtcactt gaaaactctg ggcagcccga tcgcgtttct gatcgagaac
     4681 cgggactggg aaaactggaa agagattatg gcggtggaag acaatccgga ttcagccgca
     4741 gtcgccgacc cgcgcagtgc accgcgcacc gctccccgtc cggggcacgc cgatctggcc
     4801 ggtgcgatca agtatgggca aaaagacatc cgcaacgtgc tggagcgcgc gtcagcccgt
     4861 gagacggcgg ctcgcgtcgg cgccggtgcc tttgccaaac tcttcctccg ggaattcggc
     4921 atcgatttcg ccagccacgt ggtggaaatc tgcggcgttg cggtgccgga cggacggcca
     4981 gcgttcgctg aaatccaatc cagggcaccc aagtccgagt tacactgcat cgatccggcg
     5041 acggaagaaa aaatgaaagc caaaatcgac gaggcgatag cgcgcaaaga cacggtgggc
     5101 ggagtgttcg aagtgatcgt caccggcgtg cccatcggct tggggagttt tgttcactgg
     5161 gaccgccggc tggatgctca gttggccgcc gcggtaatgt cgatcccttc ggtaaaaggc
     5221 gtggagatcg gcatggggtt cgctcaggcg caccgtttcg gctcggaggt ccacgaccag
     5281 attttttttg agggaaagac ccaatcagcg tcgcgccaga aaggtttctt tcgccggacg
     5341 aataatgcgg gcggcttgga gggcggcgtc accaacggcg aagacgtagt cctgcgcgcg
     5401 gtctgcaaac cgctttcgac
//
LOCUS       DBNGZD010000211         2138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_797339, whole genome shotgun sequence.
ACCESSION   DBNGZD010000211 DBNGZD010000000
VERSION     DBNGZD010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_797339"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /gene="selD"
                     /locus_tag="AC16GM_05585"
     CDS             <1..917
                     /gene="selD"
                     /locus_tag="AC16GM_05585"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR2923183.1"
                     /translation="DDAGVFHLTEEINLVQTADFITPPLDDPFLYGRIAAANSLSDVY
                     AMGGKPVTALNICCFPERGIEKQFLAEILQGGLDAINESGAALLGGHTIKDNELKYGL
                     SVTGICKTAELKPNSTARPGDKLILTKPIGTGVIITGSKIGRLGEDVLTRACQWMGKL
                     NAKAAQAMVEHNASSATDITGFGLAGHAYEMARASQVGIRFYASAIPHYPESLEMIRL
                     GVRTGVTQSNQEWPGETITFENGIAPEMAALFFDPQTSGGLLIAIAPERAQKLLDLLR
                     REDSDGVAIVGEVFAADTPRIEVVGEPR"
     gene            968..>2138
                     /locus_tag="AC16GM_05590"
     CDS             968..>2138
                     /locus_tag="AC16GM_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR2923184.1"
                     /translation="MSTVLSPPYDQYTDDLRERLYEKNPYNISRIISGKTLPGDDGLN
                     RYTRAKDFFLRWCQEKILIQDERPAIYPYLQRYRLGEREKKRLGFIALGRVTDFAQKI
                     VRPHERTLSEPKADRLRLRLTVKADFGLVFLLFPDPERMFQALTETVTASHPVIDAVD
                     LEGNAHQVWQVKDEKYYHELTNLLADKFTIIADGHHRYQVALDSAKMQLPGASANWWY
                     RPMAFFPMEGEGVTIYPIHRMINNLPRFSKSTLLAELEKVFEVKSAAIEELEQNQTDL
                     RAVFGRPPCGFRLSLRKGMADNLPWPPESSPAWRSLPTSVVQVAILKHLLQITDADIA
                     AEKFVTYSKDAGEVIRGVQTDQAQIGILVPPVTLEQLVAVTERGELLPPKTTFFYP"
BASE COUNT          484 a          629 c          561 g          464 t
ORIGIN      
        1 ccgacgatgc cggggtgttt catctcaccg aggaaatcaa cctggtgcaa acggccgact
       61 tcatcacccc gcccctggac gatccgtttt tatacggccg gattgcggcg gccaattcgc
      121 tttcggacgt ttatgccatg ggcggcaagc cggtcaccgc gctcaatatc tgctgcttcc
      181 cggagcgggg gattgaaaag caatttctgg ccgaaatcct ccagggcggg ctggacgcca
      241 ttaacgagtc gggcgcggcg ttgctcggcg gccacacgat aaaagacaat gaactgaaat
      301 acggcctctc agtcaccggc atttgcaaaa ctgcggaatt gaagccgaat tccaccgccc
      361 ggcccgggga taagttgatt ttaaccaagc cgatcggcac cggcgttatc atcaccggct
      421 ccaagatagg tcgtctcggc gaggacgtgc tgacccgcgc ctgccagtgg atgggcaaac
      481 tgaacgcgaa ggccgcgcag gcaatggttg agcacaatgc gtcctctgcc acggatatta
      541 ccggcttcgg tttagccggc cacgcgtatg aaatggcccg tgcgagccaa gttggaatcc
      601 gtttctacgc atcggccatc ccgcactacc ccgaaagttt agaaatgatc cgcctcggcg
      661 tacggaccgg cgtgacacag tcgaatcagg aatggccagg ggagaccatc acctttgaaa
      721 atggcattgc ccccgagatg gcggcgcttt tctttgatcc ccagacttcc ggcgggcttt
      781 tgattgccat tgcaccggag cgtgcgcaga agttgcttga tctcctccgc agggaggaca
      841 gtgatggcgt cgccattgtg ggcgaagttt ttgctgcgga tacgccccgc attgaagttg
      901 taggagagcc ccgatagaaa tccgcccctt ccgcgccttt cgctacaatc cggaaaatat
      961 taaagacatt agcacggtcc tctcgccgcc ctacgaccag tacaccgacg acctgcgcga
     1021 gcggctgtac gaaaagaatc cgtacaacat ttctcgaatt atttccggca aaactttgcc
     1081 gggcgacgat ggcctgaacc gctacacgcg ggccaaagac ttcttcctcc gttggtgtca
     1141 ggaaaagatt cttattcagg acgaacgccc cgccatttat ccttatctcc agcgttaccg
     1201 tttgggtgag agagaaaaaa aacgcctcgg cttcatcgcc ttaggccggg tcaccgattt
     1261 cgcacaaaaa atcgtccgtc cccatgagcg cactctctcc gaacccaaag ccgaccgttt
     1321 acgccttcgc ctgaccgtca aagccgattt cggcctggtc tttttgttat ttcccgatcc
     1381 ggagagaatg ttccaagcgt tgaccgaaac ggtaaccgcc tctcatccgg tcatcgatgc
     1441 cgtcgatctg gaaggcaatg cccaccaggt ctggcaggtt aaagatgaga aatattatca
     1501 cgagttgacg aatctcctcg ccgacaaatt cactattatt gccgacggcc accaccgcta
     1561 tcaggtggcg ctggattcgg ccaaaatgca gctcccgggc gcctccgcaa actggtggta
     1621 ccggccgatg gcgtttttcc cgatggaggg tgaaggggtg accatttacc cgattcaccg
     1681 aatgatcaat aatctcccgc gcttttcgaa atcaacgctc ctcgccgaac ttgagaaagt
     1741 atttgaagtt aaatcagctg caatcgaaga actggaacaa aaccagaccg acctccgagc
     1801 ggtcttcggc cgtcctcctt gtggctttcg actttcgtta cgaaaaggga tggctgacaa
     1861 tctcccctgg ccgccggaat cttctcccgc ctggcgctca ctacccacca gcgtcgtgca
     1921 ggtggccatt ttgaaacatc tcttgcagat taccgacgca gacattgcgg cggagaaatt
     1981 tgtgacttat tcgaaagatg ccggggaagt tatccggggt gtacaaacgg atcaggcgca
     2041 aattggtatt ctcgtcccac cggtcacgct ggagcaatta gttgcagtta ccgagcgggg
     2101 cgaactcctc ccgcccaaaa cgaccttctt ttatccga
//
LOCUS       DBNGZD010000212         1206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1700354, whole genome shotgun sequence.
ACCESSION   DBNGZD010000212 DBNGZD010000000
VERSION     DBNGZD010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1700354"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1206)
                     /locus_tag="AC16GM_05595"
     CDS             complement(<1..>1206)
                     /locus_tag="AC16GM_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HIR2923185.1"
                     /translation="LLAGGAPDVSYILLKPGESLRPQGIWQVRAGAKVRRLKGEVFTE
                     RGVYRPGETVHFKGLVREYLDRRIVSPVGDVCAFEITSPKGEKVYSEEKTLSEFGGAA
                     GEVTAAGHWPRGTYTLKMAFGPQEEAEPAAPKARRERSDEDEDSNDNQTTNDEADNKG
                     PKNEASCTFQVQEFKAPRHFVEIDFKRLSRAETGYVNRKLQQDFVKIGVTGAYYAGGP
                     VKHGQVRWKVHKAKTSYRVPGFDNFDFGYYRDEPGELIESGQAILDEKGRTELEFPLD
                     RQVLSGESGYLVIATVVDFDGRAASNSKDYQVEPDYLVGLSSHHGEVQADTEQTLKVV
                     AVTKNGKRISKGQIRAEVLEKSFAYVAKRNEQGDLYWSDQETWRKTYATNLPLEKGEA
                     TFRFDFNWY"
BASE COUNT          196 a          392 c          346 g          272 t
ORIGIN      
        1 cgtaccagtt gaaatcgaac cggaaggtag cctcgccttt ctccagcggc aggttggtgg
       61 cgtaagtctt gcgccaggtt tcctggtccg accagtaaag gtcgccctgc tcgtttctct
      121 tggccacgta agcgaaactc ttctccagca cctcggcccg gatctgccct ttgctgatgc
      181 gcttgccgtt tttggtcacg gccaccactt tcagggtctg ctccgtgtcg gcctgcacct
      241 ccccgtggtg gctgctgagg cccaccagat agtcgggctc cacctgataa tccttgctgt
      301 tggaggcggc ccggccgtcg aaatccacca ccgtggcaat gaccaggtag ccggattcgc
      361 cggaaagaac ctggcggtcc aggggaaatt ccagttcggt gcggcctttt tcgtccagga
      421 tggcctggcc gctctcgatg agttcgcccg gttcgtcgcg gtaatagccg aaatcgaaat
      481 tgtcgaagcc gggcacccgg tagctggtct tggccttatg caccttccag cgcacctggc
      541 cgtgcttcac cggcccgccg gcatagtaag cgccggtgac gccgatcttg acaaagtcct
      601 gctggagctt tcgattaaca tagccggtct cggcccggct caggcgctta aagtcgatct
      661 ccacaaaatg ccggggcgcc ttgaattcct ggacctggaa ggtgcaactg gcctcgtttt
      721 tcggcccttt attgtcggcc tcgtcattgg tagtctgatt atcattgcta tcctcgtcct
      781 catccgaacg ttcccgccgc gctttgggcg ctgccggctc cgcttcctcc tggggaccga
      841 aggccatttt cagggtgtag gtgccccggg gccagtggcc ggccgcggtc acctcgccgg
      901 ccgcgccgcc gaattctgac agggtctttt cctcggaata gaccttttcc cctttgggac
      961 tggtgatttc aaaagcacaa acgtctccta cgggtgagac tatacgccgg tccaggtatt
     1021 cccgaacgag ccccttgaaa tgcaccgtct ccccgggacg ataaaccccc cgctcggtga
     1081 agacctcccc cttgaggcgc cggaccttgg ccccggcccg aacctgccag attccctggg
     1141 gtctaaggct ttcaccgggt ttgagcagga tgtaggagac gtcgggcgcc cctccggcca
     1201 gcaggc
//
LOCUS       DBNGZD010000213         6225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1352905, whole genome shotgun sequence.
ACCESSION   DBNGZD010000213 DBNGZD010000000
VERSION     DBNGZD010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1352905"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="AC16GM_05600"
     CDS             complement(<1..295)
                     /locus_tag="AC16GM_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR2923186.1"
                     /translation="MRWVYFNGAFVPAEQAKISIQTHAFNYGTAIFEGMRAYWNAEKK
                     RLFGFRWEAHFRRMEAGCRVLKIKLPQPDADLINLTGELLRANDCREDVYIRPL"
     gene            complement(306..1166)
                     /locus_tag="AC16GM_05605"
     CDS             complement(306..1166)
                     /locus_tag="AC16GM_05605"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR2923187.1"
                     /translation="MSLRNLKVAIIGLGQIGGSLGRDLVFGKVVKEVIGSDRDRVTRR
                     WSMRTKAVHRVADSLSGAVRSADLVVLAVPVRRIVSLLPQVAQHLKPGAVVFDVGSTK
                     REIVRAMERLPRGILACGGHPMAGTEKSGIRAAQKNLFTDKAFFLVPTRKTKPESLAL
                     CRRLVRAVGARPVVISAAAHDRLLATTSHLPYLLAVALLERGIQVARRRRDFWSLLGG
                     GFASATRVAKSNPELMLDILASNRTNVASVVDDFGRTLKRYARALRSKNDRRLRRSIN
                     CAFTASRFIR"
     gene            complement(1163..1537)
                     /gene="aroH"
                     /locus_tag="AC16GM_05610"
     CDS             complement(1163..1537)
                     /gene="aroH"
                     /locus_tag="AC16GM_05610"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR2923188.1"
                     /translation="MVRGIRGAVTVKENSRRAIFAATQKLLRAMVRDNHVTRDDIASV
                     FLTATEDLNVEFPAYALRQMGWKTVPILCAREMGVPGSMRKVIRALIHVNMDKAQPEI
                     KHQYLGGAKKLRPDLFRRSGRK"
     gene            complement(1755..2711)
                     /gene="cysK"
                     /locus_tag="AC16GM_05615"
     CDS             complement(1755..2711)
                     /gene="cysK"
                     /locus_tag="AC16GM_05615"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR2923189.1"
                     /translation="MSEIKPSILDLIGKTPLVRLNRLTRDVPVEIWAKLEFFNPSGSV
                     KDRIALAMIAEAEKKGLIRPGATIIEPTSGNTGISLALVCAVKGYKMIAVMPGAMSSE
                     RAAMMKLLGARVEIVPTRDNVPGLFTKEDIDNTIRRAQEIQAETPGAYVPNQFENPDN
                     PHIHALTTAREIIDQTVGKFQAFVAACGTGGTFCGVAAVLKEQFPQIRRVVVEPKESA
                     VIAGGQPGPHKIQGIGEGFIPKVMDVTLVDEAIGVSDADAILTAKRLAAEEGVLTGLS
                     GGANVFAALEVGKKMKKGEVVVTIIPDNSLRYLSTELCRCQE"
     gene            complement(3163..>6225)
                     /locus_tag="AC16GM_05620"
     CDS             complement(3163..>6225)
                     /locus_tag="AC16GM_05620"
                     /inference="COORDINATES: protein motif:HMM:NF046853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2923190.1"
                     /translation="FAILQQVIANAVALSAGVSWLSANPDSGVVPAGSSVAVEITFDA
                     TGLNGGDYYANILIASNDPDEPEDTVPAHLHVTGAPDIAVSPSSLAYGSVYTGVGATK
                     TLRVKNVGTDLLTVSNIASDNAAFSPNTTAFSLNPNDSQNVLVTFLPNDTGLFTGNLT
                     ISSDDPTDPTLPVPLQGRGIYPPVLTVFPDSFGFRLGQNDSAHTTLAIGNTGLGDLQW
                     NISVGGSAVARPRTYPERYYHSLPKGATDDRVGNPVLLRSGGPDNFGYSWIDSDEPGG
                     PVFNYRDISATGVQVFGLTDDNFVGPFLLGFTFNYYGTDYTQFYIASNGFIGFGPTDS
                     YSSLSNSPIPNSSPPNNILPWCWDDLFPQATSAVYYQTIGSQLIIQFKNYGEFGGPGL
                     VNAEVILNADGTILYQYLNFLNGFDKLSSTVGIENQNGTDGLEVVFDNSYLKDTLAVL
                     FSANQWLSAAPVSGTVPPGGSVNVDVKVKTENLTPGRTYRGNLLLTGNDPLRPQVPVP
                     VRLDVVWVPNIVLSSASLDFGEVYIGYHETSQVRISNTGLDSLKVSGMDISDPQFSLL
                     DPAILALAPGEYRDTRLQFSPTGAGNQSATLTIHSNDPNDSTVTVQLRGKGVQPPAID
                     VVEDSLAFRLREGQSQSAMLTIENSGLGPLFWTLAIGGPGSVSAAPAVSAASDATSTF
                     KGVPDERESTPVITAAASVPNAFLYTWLDSDAPGGPVFNWIDISGTGTFVSGLFDNNF
                     VGPFPIGFSFNYYGTDYTQFYISSNGFIGFGPTSGYDSPANTPLPSGSSPNNILAWCW
                     DDLIRRSGSVFYRSAGNQLIIQFINYGQVGVTTAKVNAEVILSSDGSITYQYLNFQSG
                     FNVAGSTVGIENSDGTDGLQIAHNQSYLHDGLAILIARGLFVAPLSGTIPPGGSQDVT
                     VRVKTNHAGIGMFRASLLVNSNDPARPQETVPVKVEITPCLVHGDVNNDALTDLADIT
                     ALIQDVVFASGLPNPDAGDIDCDGIRDLVDVVGLIGFVVYGSPTPCCL"
BASE COUNT         1301 a         1779 c         1822 g         1323 t
ORIGIN      
        1 ccagcggccg gatgtaaacg tcttcccggc agtcgttggc gcgcaaaagt tcccccgtaa
       61 ggttgatcag gtcggcgtcc ggttggggca atttgatctt cagcacccgg cagcccgcct
      121 ccatccgccg aaagtgcgcc tcccagcgga aaccgaacag tctctttttt tcagcgttcc
      181 agtaggcccg cattccttcg aaaatcgccg tcccgtagtt gaaggcgtgc gtctggatgg
      241 aaatcttcgc ctgttcggcg gggacaaatg ctccattgaa atagacccag cgcatcgtaa
      301 gtccgttacc gaatgaaccg gctggcggtg aatgcacagt ttatgctccg acgcaacctg
      361 cggtcatttt tgctccgcag cgctcgcgcg tatcgcttca gcgtgcgtcc gaaatcatcg
      421 accacgctgg caacattcgt gcggttggag gcaaggatat ccagcatcag ctcgggattg
      481 cttttcgcca cccgcgtggc gctcgcaaag ccgccgccta agagtgacca gaaatcccgg
      541 cgccgtctgg caacctggat tcctctctcc aaaagcgcca ccgccagaag atagggcagg
      601 tggctggttg tggccaacag ccggtcgtgc gcggcagccg aaatgactac cggacgggcg
      661 ccgacagcgc ggaccaatcg acggcacagc gcgagtgatt cgggtttggt cttccgggtc
      721 ggcaccagga aaaatgcttt gtcggtaaaa agatttttct gtgccgctct aatccccgat
      781 ttttccgtcc ccgccatcgg gtgtccgcca caggccagta tcccgcgcgg gagacgctcc
      841 attgcccgga caatttccct tttcgtgctc ccgacgtcaa agacaacggc tccgggcttc
      901 aggtgttggg cgacctgcgg taaaagggaa acgatccggc gcacgggtac ggcgagaacg
      961 accagatcgg cggacctgac cgcaccggag agcgagtcgg caacccgatg gactgctttt
     1021 gtacgcatcg accaacggcg tgtaacgcgg tcgcgatcgg aaccgatcac ttccttgact
     1081 actttcccga agaccagatc tcgccccagc gaaccgccga tctggcctaa gccgatgatg
     1141 gcgaccttaa gattgcggag gctcattttc gtcccgaccg ccggaacaaa tcgggccgga
     1201 gttttttcgc cccgcctagg tactggtgct tgatctctgg ctgcgctttg tccatgttga
     1261 cgtggattaa agcccgaatc accttccgca tgctgcccgg tacccccatt tcccgggcgc
     1321 agaggattgg caccgtcttc caccccattt gccggagggc gtaggccgga aattccacgt
     1381 tcaggtcctc cgtcgccgtg aggaagacgc tggcgatgtc atctctcgta acgtgattgt
     1441 cgcgcaccat cgctcgcaag agtttttgcg tcgccgcaaa aatcgcccgg cgtgaatttt
     1501 ccttgaccgt caccgcgccc cgaatgcctc tgaccatttt gctcctttct tgccgttccc
     1561 gaaacaaaaa agcccgctgt cttttccggc agtgggctgg tggccgtcaa aaacaataag
     1621 tcacgaataa cccactggag tgggaagcga ataataatac caatgtgccc ggatatgaaa
     1681 gaacggtctg tgcatcggtc gtttcttaat aaaacagcta cacgtagccg aaagttccac
     1741 gacccgggcc attcttattc ctggcaccgg cagagttccg tagagagata gcgcaggctg
     1801 ttatcaggaa taatcgttac cacgacttcg cctttcttca tcttttttcc gacctcgagg
     1861 gctgcaaaca catttgcgcc gccggaaagg ccggtcagga ccccttcctc cgccgccagg
     1921 cgcttggcgg tgagaattgc gtctgcgtcg cttaccccga ttgcctcatc gaccagggtt
     1981 acatccatta ccttggggat gaacccttcg ccgatgccct ggattttgtg ggggccgggc
     2041 tgcccgccag caatgaccgc tgactccttt ggttcgacca ccacccgccg gatctgcggg
     2101 aactgctctt tgaggaccgc cgccacgccg cagaaggtcc ctcccgttcc gcaggccgcc
     2161 acgaaggcct ggaatttccc gacggtctgg tcgatgatct cgcgggcggt ggtgagggca
     2221 tgaatgtggg gattgtccgg gttttcaaac tggttgggaa cgtatgcgcc cggcgtctct
     2281 gcctgaattt cctgggctct ccggatggtg ttatcgatgt cttccttggt gaacaagccg
     2341 ggtacgttgt cccgggttgg gacgatctcg acccgcgcac ccaaaagttt catcatcgcc
     2401 gcccgctccg agctcatggc gcccggcatc accgcgatca ttttgtaccc tttcactgcg
     2461 caaaccaggg ccagactgat tccggtattt cccgaagtgg gctcaatgat agttgcgccg
     2521 ggtcggataa gccctttttt ctcggcttct gcgatcatcg ccagagcgat ccggtctttg
     2581 acgctgccgc tgggattgaa gaattcaagc ttcgcccaga tctcgaccgg gacgtcgcga
     2641 gtgagccgat tgagccggac cagaggcgtt ttgccgatga ggtcgaggat gctcggctta
     2701 atttcactca cgggacttgc agaatctttt agcgggtgcg ggaagatact gcgccgctgg
     2761 ggccgcgatt cactactatg tcgagtctgc aattgcataa gcccagagtt gcaagacggc
     2821 aagaaacgga cccagcatag cccttggacg aaaaaaggca agttggggga ctacatttgt
     2881 cttcattcgt ctcggggatg acgacccata attccccctt ttctaacgat ttcttttttt
     2941 tctggcgggg gtttcagaaa gccgtaggga ggcaccggta atgaagtcag tttgaactga
     3001 aagctgaccc tcgtacgtta ccggcgccaa atcgcacctg caagttactg caatgctact
     3061 accaaaatcc cggtaatcag taatagcccc acagggctat gaccgattct tggaacttgc
     3121 cgtacctttt tcaatcagga catttgaaac tgtttcaact ctctacaaac aacacggcgt
     3181 cggactcccg tacaccacga acccgatcaa acccaccacg tccaccagat cgcgaatgcc
     3241 atcgcagtca atatctccag catcgggatt cggcaggccg ctcgcaaaga ccacatcctg
     3301 gatcagggca gtgatatccg ccaggtcggt caatgcgtca ttgttcacat ccccgtggac
     3361 gaggcagggc gtgatctcaa ctttcaccgg tacagtctcc tgtggtcggg cggggtcgtt
     3421 gctgttgacg aggagactgg cgcggaacat cccgataccc gcatggttgg tcttcactcg
     3481 caccgtaacg tcctgcgacc cgcccggcgg gattgttccg ctgagtggcg cgacaaagag
     3541 accccgcgca atcaaaatgg ccaggccgtc gtgcaagtag ctctggttat gagcaatctg
     3601 caacccatca gtgccgtcgc tattctcaat gcccaccgtg ctgccggcga cgttaaagcc
     3661 gctctggaaa ttcaggtact ggtaggtaat actgccatcg gaactcagga tgacttccgc
     3721 attgaccttg gcggtggtca cgcccacttg cccgtagttg atgaactgga tgatcagttg
     3781 attgcctgcg cttcggtaga aaaccgaacc gctccgtcgg atcaggtcat cccagcacca
     3841 ggccaagata ttgttcggag acgaaccact cgggaggggg gtattcgccg ggctgtcgta
     3901 cccactggtc ggaccgaagc cgataaaccc gttggaggaa atgtagaact gggtgtaatc
     3961 ggtcccgtag tagttgaacg aaaaaccaat ggggaaaggc ccgacgaaat tgttatcgaa
     4021 cagcccgctg acaaacgttc ccgttcccga aatatcgatc cagttgaaca cgggtccgcc
     4081 cggtgcgtca ctatccagcc aggtgtagag gaaagcattc ggcacgctgg cggctgccgt
     4141 gatcaccggt gtactctcgc gttcgtcggg aacacctttg aaggtgctcg tggcatcaga
     4201 cgcagcggag actgccgggg ccgcggacac tgacccgggt ccgccgatag cgagggtcca
     4261 gaacaagggg cccaggccgc tgttctcaat agtcaacatc gccgactgac tttggccttc
     4321 gcgtaacctg aaggcaagtg aatcttctac gacgtcaatc gccggcggct ggacgccttt
     4381 accccgcaac tggaccgtga cggtcgaatc gttcgggtca ttgctgtgaa tggtgagcgt
     4441 cgcgctctgg ttgcccgctc cggtggggct gaattggagc cgggtatccc ggtactctcc
     4501 gggggccaga gcgagaatcg ccggatcgag gagggaaaat tgcggatcgc tgatgtccat
     4561 cccgctcact ttcagcgagt ccagaccagt attggaaatg cgcacctgcg aagtttcgtg
     4621 atagccgatg tagacctccc cgaagtcgag ggaggcactg gagagtacga tgttgggcac
     4681 ccagacgacg tccaagcgga ccgggacggg aacttgcggt cggagcgggt cgttgccggt
     4741 gaggagcagg ttgccacggt aggtgcggcc gggagtcagg ttctcagttt tgacttttac
     4801 atcaacgttg acgctgccgc cgggtggaac ggtgccgctc accggcgcgg ccgacagcca
     4861 ttgattggcc gagaacaaaa cggctaaggt gtccttcagg tagctgttgt caaaaacaac
     4921 ttccaagcca tccgtgccat tttggttttc gatgcccacc gtgctgctca atttgtcaaa
     4981 gccgttcagg aaattcaggt actggtagag aatggtgccg tcggcgttca ggatcacttc
     5041 ggcattgacc agaccgggcc caccgaattc gccgtagttt ttaaactgaa taatcaattg
     5101 actcccgatg gtctggtagt aaaccgccga agtggcttgg gggaagagat catcccagca
     5161 ccagggcaaa atgttattgg gaggggaact attcggtatg ggcgaattgc tgagggagga
     5221 atagctgtcg gtggggccga agccgataaa gccattggag gcgatgtaga actgggtgta
     5281 atcggtcccg tagtagttga aggtgaatcc gaggaggaac ggccccacga aattgtcgtc
     5341 ggtcagtccg aagacctgca cgcccgtggc cgaaatatcc cgatagttga agacgggacc
     5401 gcccggctca tcgctatcga tccaggagta gccgaaattg tcgggaccgc cgctgcgcag
     5461 gagcaccggg ttccccaccc ggtcgtcggt tgcgcctttg ggaagactgt gataataacg
     5521 ctcagggtag gttctgggtc tggcgacggc tgatccgccg accgaaatat tccattgcaa
     5581 atcacccaat cccgtgtttc ctatcgccag cgtggtgtgc gcgctgtcgt tctgacccaa
     5641 ccggaaccca aacgaatcgg ggaagaccgt caggaccggg ggataaatgc cccggccctg
     5701 cagcggcaca gggagagtcg gatcagtcgg gtcgtcgctg gagatggtga gattgccggt
     5761 gaataacccg gtatcgttgg gcaggaaggt gaccaaaacg ttctggctgt cgttgggatt
     5821 caaactgaac gcggtcgtgt tcgggctaaa ggcggcattg tcggacgcaa tgttactcac
     5881 cgtcaaaagg tcggttccga cgtttttgac ccggagcgtc ttggtcgcac ccactcccgt
     5941 gtacacggag ccgtaggcca gggaagaggg cgatacggca atatcgggtg cgccggtgac
     6001 gtggaggtgg gcgggaaccg tgtcttccgg ctcgtccggg tcgttgctgg cgatgagaat
     6061 gttggcgtag tagtcgccgc cgttcaagcc ggtggcgtca aaggtaattt caacagcaac
     6121 cgacgagcca gccggaacaa cgccggaatc gggattcgcg gaaagccagc tgacacctgc
     6181 tgagagagcc acggcgttgg cgatcacctg ttgcaggatc gcaaa
//
LOCUS       DBNGZD010000214         1807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_103147, whole genome shotgun sequence.
ACCESSION   DBNGZD010000214 DBNGZD010000000
VERSION     DBNGZD010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_103147"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="AC16GM_05625"
     CDS             complement(<1..557)
                     /locus_tag="AC16GM_05625"
                     /inference="COORDINATES: protein motif:HMM:NF040068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR2923191.1"
                     /translation="MLVTEGKSIATVSLKEKIEKEFLPFVIKPGRYIGNELGATHKDP
                     EEKVRFCLAFPDAYELGMSYLGMGILYHLINELPYARAERVYAPWVDAGELLRRKNIP
                     LFSLESRTPLKAFDAVGFSISYELTFSNLLSMLDLAGIPILSSRRDEDDPIILAGGTA
                     VVNPEPVADFIDLFVLGDAEEIITK"
     gene            complement(676..>1807)
                     /locus_tag="AC16GM_05630"
     CDS             complement(676..>1807)
                     /locus_tag="AC16GM_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2923192.1"
                     /translation="SNFGQFGFGSGSEIEFAGGQGFFYPRLVGLNNLFELALLMGTSD
                     STVSNAARGDFSSILIDRHFVPIAGGNLTRLTPGPQADEESFARFNDSGAPRPLGVEI
                     TQNIMSFTAANYDDFAILEYRIKNVSGNALNGLRVGLFADWDFPWGSGASDSVDFDAT
                     RNLGYMMHATLRQPRGLAVLTPPGATSYRAINNPDSIYDGFSMGEKWRFMSQGVAQVR
                     SGGGDRAHLISTGPFNLLPGQTDTVAFAVVGGGNVSALQANTDSARSKYLAITTEVKE
                     REVSAPLPEQFALSQNYPNPFNPNTVIRFALPKASEIELSVYNLLGQKVATLATGLRS
                     AGVHEVVWDGTDAFGHPAASGLYFYRLKAGDFTDARKMLLLK"
BASE COUNT          406 a          491 c          553 g          357 t
ORIGIN      
        1 attttggtaa tgatttcttc ggcgtcaccc agaacaaaaa ggtcgatgaa gtcggccacc
       61 ggctccggat tgactaccgc cgtgccgccc gctaggataa tcggatcgtc ctcatcccgc
      121 cgggaagaga gaatcggaat acccgccaag tcaagcatgg agaggagatt cgaaaaggtc
      181 agctcgtaag agatggaaaa gccgaccgcg tcgaaagctt tcagcggtgt ccggctctcc
      241 agtgaaaaga gcgggatatt tttcctgcgg agcagttccc cggcatcgac ccagggagcg
      301 tagacccgtt cggcccgggc ataggggagt tcgttgatca aatggtagag aatgcccatc
      361 ccgaggtacg acatccccag ttcgtaggcg tccgggaagg ccaggcagaa gcgcaccttc
      421 tcctccgggt ccttgtgggt ggcgcccagt tcgttgccga tgtagcgccc cggcttgatg
      481 acgaaaggga gaaactcctt ttcgattttc tcttttaagg atacggtggc gatgcttttc
      541 ccctcggtaa ccagcattag gaagcctgta ttccctccac ggagtgtaaa gaaattaccg
      601 ttttggtctc actttgtaaa gcgggcaaaa agaaaccccg ccgaggcggg gtttccattc
      661 cggcatacgg gcacatcatt tcaagagcag catttttctc gcgtcggtga aatcacccgc
      721 cttgagccgg tagaagtaga ggccggaggc cgccggatgg ccaaaggcgt cggtaccgtc
      781 ccagacaact tcgtgaaccc cagctgatcg cagacccgtg gccagggtgg ccactttttg
      841 ccccaaaagg ttgtagactg agagttcaat ctcgcttgct ttgggcaagg caaagcgaat
      901 gaccgtgttc gggttgaacg ggttggggta gttctgagag agggcaaact gctcaggcaa
      961 aggggcggag acttcgcgct ctttcacttc ggtggttatg gccaggtact tcgaacgggc
     1021 cgaatcggta ttcgcctgaa gggctgacac attgccgcca ccgacgaccg caaaggccac
     1081 cgtgtctgtt tgaccgggaa gcaggttgaa ggggccggtg gaaatcaggt gggcgcggtc
     1141 gcccccgccc gagcggactt gcgccacccc ctggctcata aagcgccact tttcacccat
     1201 ggaaaagccg tcgtaaattg aatcggggtt gttaattgcc cggtaggacg tggcgccggg
     1261 cggcgtcaga accgccagcc cgcgaggctg cctaagggtg gcgtgcatca tgtaacccag
     1321 gttgcgggtg gcgtcaaaat ccaccgaatc agaagcaccc gaaccccagg gaaaatccca
     1381 atcggcaaag agcccgaccc gcaggccgtt gagcgcattg ccggatacat ttttaattcg
     1441 atattccaga atggcgaagt catcgtagtt ggcagcggta aaggacatga tgttctgggt
     1501 aatctccact cccaaaggcc gcggcgcgcc ggagtcattg aaccgggcaa aagactcttc
     1561 gtcggcttgc ggcccgggcg tcaggcgcgt cagatttccg ccggcaatcg gcacgaaatg
     1621 gcggtcgatg aggatggagc tgaagtcacc gcgggcggcg ttggaaacgg tgctgtcgct
     1681 cgttcccatc aagagtgcca gttcaaagag gttgttcagg ccgacgagtc tggggtagaa
     1741 gaacccttga ccaccggcaa actcgatttc ggaaccggag ccaaagccga actgcccgaa
     1801 gttggag
//
LOCUS       DBNGZD010000215         4249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1909081, whole genome shotgun sequence.
ACCESSION   DBNGZD010000215 DBNGZD010000000
VERSION     DBNGZD010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1909081"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="AC16GM_05635"
     CDS             complement(<1..420)
                     /locus_tag="AC16GM_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2923193.1"
                     /translation="MHIDPHTLKSFGRNAASGEAIYQQGEPAEKLFGLVSGQVKLVRT
                     VCGKSLTVALVGPGEFFGAVEFLTHRPFLCTARAEVAAQIVAVNRPFVESYLLAHPKV
                     MHRLLVQLAHQQALLIRQIENLLSTDIAYSAINTLVGL"
     gene            complement(548..1558)
                     /gene="lipA"
                     /locus_tag="AC16GM_05640"
     CDS             complement(548..1558)
                     /gene="lipA"
                     /locus_tag="AC16GM_05640"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR2923194.1"
                     /translation="MEVAEVTSNAHDITLISRGTQRHPYFVHPEYAQPGQAVRVARKP
                     TWLKVTPPLGQNYRRLKAMLGGMKLHTVCQEANCPNIGHCYEKGTATFMILGDLCTRG
                     CRFCDVKRGKPGPVDPEEPKRLAEAAGKMGLRHVVITSVTRDDLSDGGASQFAACIRE
                     TRKTLPRSTIEVLISDLRGSREALEIVLAACPDVLNHNVETVPRLYTRVRLGAMYNRS
                     LELLARSKEIAPHILSKSGIMLGLGERRPEIEKTLWDLRGAGVDILTIGQYLSPSAWH
                     LPVEEYVHPDVFEELRVFGLSLGFREVYSGPLVRSSFNADEQLERAIGALPVTNGHLP
                     SE"
     gene            complement(1684..2667)
                     /gene="trxB"
                     /locus_tag="AC16GM_05645"
     CDS             complement(1684..2667)
                     /gene="trxB"
                     /locus_tag="AC16GM_05645"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012960403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR2923195.1"
                     /translation="MAKDKIYDVVIVGGGPAGLCAGLYTSRANLDVVLLEKLLPGGEI
                     TNTEWVEDYPGFDLIGGPELAKKMEEHAKKFGLKIEMQNVTEIYSEGLIKVVKTDDGE
                     YRSRTVIFCPGGSPNKLEIPGEKELAGKGVSYCAICDGAFFKSQVIAVVGGGDAAVEE
                     GTFLTKFGSKVYLIHRRDRFRAQKIIQERAFANPKIEVIWNTVVEEISGDSKVEHLRL
                     KNVATGEKKRLDVGAVFIFVGFVPNSHLIKDRVEKDKNGYVVTNDRMETNVPGLFVCG
                     DVRAQLCRQVTNAVGDATTAAVAAEKYLVKLKDAERSGRPVGQGQPLAVTK"
     gene            complement(2820..>4249)
                     /gene="glmS"
                     /locus_tag="AC16GM_05650"
     CDS             complement(2820..>4249)
                     /gene="glmS"
                     /locus_tag="AC16GM_05650"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR2923196.1"
                     /translation="EEHYEGNLLEATRAALSLVEGTYGIAVISRNHPGEIVAARHGSP
                     LLVGIGAGENFVSSDAAAILRHTKNVVYLEEKQIAHITRTSYAISTIDNVSITPHVQE
                     ISWSLDMIEKEGFDHFMQKEIFEQSTTLRNAMRGRLNPPEGTARLNGLLLRYDKLPQI
                     NRVIITACGTSWHAALIGEYLIEELARIPVEVEYASEFRYRSPILNDGTVVLVISQSG
                     ETADTLAAMREAKHRGAPVFGICNVVGSTIARETDGGVYIHAGAEIGVASTKAFTAQV
                     MVLSLIALMLGRMRSLPPEKGKELLAGLQQVPELVQRILNTDQKIKEIAQKLYQKNNF
                     LYLGRGINFPVALEGALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVVVIALKDAV
                     YDKVISNIQEIRARRGRVIAIATEGDADIARLADFVISIPPVADPLTPMLSVIPLQLL
                     AYHMAVLRGCNVDQPRNLAKSVTVE"
BASE COUNT          845 a         1273 c         1211 g          920 t
ORIGIN      
        1 gagccccacc agcgtgttga tggcgctgta ggcgatgtcg gtggagagga gattttcgat
       61 ctgccggatc aaaagcgcct gctggtgggc caactgcact aaaagccggt gcattacctt
      121 ggggtgcgct aagaggtaac tttccacaaa gggacgattg accgcaacaa tttgcgcggc
      181 aacttccgca cgggcggtgc acaagaatgg ccggtgggtg aggaactcga cggccccgaa
      241 aaactccccg ggaccgacga gggccacggt cagacttttg ccgcagaccg tgcggaccaa
      301 tttcacctgg ccggaaacga ggccgaacag tttttccgcc ggctcgccct gctggtagat
      361 ggcttccccc gaggctgcgt tccgaccaaa ggacttgaga gtgtgcgggt caatgtgcac
      421 gatttctttc ccgcgtggtc attccatcct gcgcaggcac caagattttc gccgtgcttg
      481 cgacgcaagg taacaacatc cggtcgcccc ctgcaactcc tctgtatttt gaagagttac
      541 cgatttacta ttcgctggga aggtggccgt tcgtaaccgg aagagcaccg atcgcccgct
      601 ccaactgctc atccgcgttg aaggaactgc gtacgagcgg cccggagtaa acctcgcgga
      661 aacccagcga gagcccgaag acccgcaatt cttcgaaaac atcggggtgg acgtattctt
      721 ccaccggcaa atgccaggcc gagggggaca ggtactgacc gatggtgagg atgtcgacgc
      781 ccgcgccgcg caagtcccag agtgtctttt cgatctccgg acgcctttcg cccaaaccga
      841 gcataatgcc ggacttggac agaatgtgcg gagcgatctc ttttgagcga gccagaagtt
      901 ccagcgagcg gttgtacatg gcccccaaac ggacgcgcgt atagagacgc ggcacggttt
      961 caacgttgtg attcaaaacg tccggacagg ctgccaacac gatttctaat gcctcgcgcg
     1021 agccgcgcag gtcggaaatc aaaacttcga tggtgctccg gggaagcgtc ttgcgcgttt
     1081 cccggataca agcggcaaac tgggaagctc cgccgtccga tagatcatca cgggtgaccg
     1141 aggtaatcac cacgtgccgc aaacccattt tgccggcggc ttcggccaat cgcttgggct
     1201 cttccggatc gaccggcccg ggcttgccgc gcttgacgtc gcagaagcgg cagccgcggg
     1261 tgcagagatc gcccagaatc atgaaggtgg cggttccttt ttcgtagcag tggccgatgt
     1321 tggggcagtt ggcctcctgg cagacggtat ggagcttcat cccccccagc atcgctttga
     1381 gacgccggta gttctgtccc agaggcggcg tgactttcag ccacgtcggc ttccgggcca
     1441 cccgcaccgc ctgccccggc tgcgcatact cgggatggac gaaatagggg tggcgctgag
     1501 tgccgcgcga gatgagcgtg atgtcgtggg cgttgcttgt gacttcagcg acttccatgc
     1561 gagattcgag gcgcgactta agtcgcgctg tagaccgatt tctatcgcaa cctgtggcgc
     1621 aggttggtct tagaaacctg cgtccgtgcg ggataccaag accatcccgc acttcgagct
     1681 gatttatttt gtcaccgcta aaggctgtcc ctgcccgacg ggtcgtcccg accgctccgc
     1741 gtctttcaac ttcaccagat acttttccgc cgccaccgcc gcggtggtcg catctccaac
     1801 cgcattggtc acctggcggc aaagttgggc gcgcacatcc ccgcagacga acaggccggg
     1861 aacattcgtt tccatccgat cgttggtgac gacgtagccg ttcttgtcct tttccactct
     1921 gtctttgatc aagtggctgt taggcacgaa accgacgaaa atgaaaaccg cgccgacgtc
     1981 cagccgcttc ttttcaccgg tcgcgacgtt tttcaaccgc aaatgctcta ccttgctgtc
     2041 gcctgaaatc tcttcgacaa cggtgttcca aatcacctca atctttggat tcgcaaaggc
     2101 gcgttcctgg atgatcttct gcgcccggaa ccggtcccgc cggtggatca ggtagacttt
     2161 cgagccaaac ttggttaaaa acgtcccctc ttccaccgcc gcgtcgcccc cgcctacgac
     2221 cgcaatcacc tgacttttga agaatgcccc gtcgcagatg gcgcagtacg acactccttt
     2281 gccggccaac tctttttctc ccggaatttc caatttattg ggcgagccgc ccggacagaa
     2341 aatcaccgtg cgcgagcggt attctccatc gtcggttttg accactttga tcaagccttc
     2401 ggagtaaatc tcagtcacgt tttgcatctc gattttcagc ccgaactttt tggcatgctc
     2461 ctccatcttc ttcgccaatt ccggcccgcc gatcaggtca aagcccgggt aatcttccac
     2521 ccactcggtg ttggtgattt ccccgccggg gaggagtttt tctaaaagaa cgacgtccaa
     2581 gttggcccgg gaggtgtaga gcccggcgca gagcccggcc ggcccgccac ccacgatcac
     2641 cacgtcgtaa attttgtctt tggccatcgg ctaaccttca gcaagaaaga ccgttgtata
     2701 cggtctttca tcgataatac gtgccgccgt tggaaaaagt caatccggct tgaagctctc
     2761 ccgaccggca ttgaaccgga ctccctgatt cctattgttt ccgcgcgttc cgaaatgatc
     2821 tattccaccg ttacgctctt ggcgaggttg cgcggctgat cgacgttgca cccgcgcagg
     2881 accgccatgt ggtaggccaa gagctgcagc ggaatgaccg agagcatcgg cgtcagggga
     2941 tcggccaccg gcggaatgga aatgacgaag tcggccaggc gggcaatgtc ggcatcgcct
     3001 tcagtggcaa tggcaatcac ccgcccccgg cgggccctga tctcctgaat gttggagatg
     3061 accttgtcgt agactgcgtc tttgagcgcg attaccacca ccggcatatt ctcgtcgatc
     3121 aaagcaatcg ggccgtgttt catctccgcc gccgggtacc cttcggcgtg gatgtaggag
     3181 atttctttga gtttcaaagc accttccaga gccactggga aattgatccc ccggcctaag
     3241 tagaggaagt tatttttttg gtagagtttc tgggctatct ctttaatctt ctggtcggtg
     3301 ttgagaatcc gttgaaccaa ctccggcacc tgctggagtc cggcgagcaa ttctttccct
     3361 ttttccgggg gaagcgagcg catccggccc agcatcaggg caatcaggga caataccatc
     3421 acctgggcgg taaaggcctt ggtcgaggcc accccgattt ccgccccggc gtggatgtaa
     3481 acgccgccgt cagtctcccg ggcaatggtg gagccgacca cgttgcagat gccgaagacc
     3541 ggcgcacccc ggtgcttggc ttcccgcatc gccgccagcg tatccgccgt ctcgccggat
     3601 tggctgatca ccaggaccac cgtgccgtca ttgagaatcg gactccggta acggaattcg
     3661 gatgcatact ccacctccac cggaatccgg gccaactctt caatcagata ttcgccgatc
     3721 aaagccgcat gccaggaggt cccgcaggcg gtgatgatga cacgattgat ttgaggcaac
     3781 ttatcgtaac gtagcaatag gccgttcagc cgggcggtgc cctcgggggg attcaagcgg
     3841 ccccgcatgg cgttgcgcag ggtggtcgac tgctcaaaga tctccttttg catgaagtgg
     3901 tcgaaccctt ctttttcgat catgtccaga gaccaggaaa tctcctgcac gtgcggcgtg
     3961 atcgatacgt tgtcgatggt ggaaatggcg taggaagtcc gggtgatgtg ggcgatctgc
     4021 ttctcttcga ggtaaaccac gtttttggta tgccgcagga tggccgcggc atccgaggag
     4081 acgaagtttt cacccgcgcc gatcccgacc agaaggggcg agccgtgccg ggcggcgacg
     4141 atttctcccg gatgattccg cgaaatgacg gcaatgccgt aggtgccctc aacgagcgag
     4201 agggccgccc gggtggcctc gagcagattc ccttcgtagt gctcttcaa
//
LOCUS       DBNGZD010000216         4359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_312492, whole genome shotgun sequence.
ACCESSION   DBNGZD010000216 DBNGZD010000000
VERSION     DBNGZD010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_312492"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2367)
                     /locus_tag="AC16GM_05655"
     CDS             complement(124..2367)
                     /locus_tag="AC16GM_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2923197.1"
                     /translation="MVGQTISHYKILEKLGEGGMGEVYLAEDTDLNRKVALKFLPQQL
                     TADPETKLRFKQEAQAAAALNHPNIITVYEVAEYQRAGYIAMEYVAGQSLRDLAAGRG
                     LPAGKALDIAIQIAEGLEKAHQAGIVHRDIKSDNILINQDGRVKILDFGLAKLKGASR
                     LIQPGSTAGTVAYMSPEQATGEEVDQRSDLFSLGVVFYELLTGQLPFPGEHQTAILYS
                     ILNEPPQPLARFAGEVPAQLQEIVEKMLAKKREERFQSAAELVVDLRRCKKELEEGKP
                     EAPKKEKKAVAVLYFENLSAEPDSDYFSAGITEDIITDLSKIESLRVASRNAVLPYKD
                     QPVDIPLLGRKLNLDAVLEGSVRKFGNRLRITAQLIDTKEGFHLWAERYDREMQDVFV
                     LQEEIAKKIALALQVTLSEKDEEQIAMKYKGDLDAYSCYLKGRGYYYKYTKPDLLTAM
                     QMFNSALELDPQYALAHAGLSDCYAQMIDKYSETDQTFFAKAEEAGLKALRIDSRCAE
                     AYKALGVIYSKQGHNRKSKEQFLQALAIKPNYAPARASLGVVYTNLGNFAEAEKEYLL
                     AYEQDPSMTVLLYLLANFHLSVNNFSRAENYIDQLLEAGESSFYLKMGHYVKARISFY
                     QRQYESALAQMQKYVEMEPAEPIGNSGLAMIYAALGDHEKAREKLDGALKTFAWHEYV
                     IENTILTYTLLGEKGKVYEWMTKGMEANKIDWLFLEFNPLLEELRHEPEFQHHLQEIK
                     KRVLNSE"
     gene            2488..3708
                     /gene="serA"
                     /locus_tag="AC16GM_05660"
     CDS             2488..3708
                     /gene="serA"
                     /locus_tag="AC16GM_05660"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR2923198.1"
                     /translation="MAKGTRPAIKVLLLEKINPGAEEQFRAQKYRVTLRAGALSEPEL
                     AGVLGPVQILGIRSKTQVTPKILQQAENLLAIGCFCIGTDQVDLRACTERGIAVFNAP
                     YSSTRSVTELVIGEIIMLLRRVTERSTAAHHGRWLKSDDSSFEVRGKTLGIFGYGHIG
                     SQVSVIAEALGMNVIYYDILDKLSLGNARSVHSLSELLRRADIVTLHVPEDKTTRHMI
                     GAREMAQMKKGSYLINTSRGSVVVLEDLYRALVDGHLRGVAIDVYPNEPSGPNARFTC
                     LLQGLPNVILTPHIAGSTQEAQADIAQKTSSRLIRFLEFGTTTGSINFPEVELPVLNQ
                     NHRIIHIHRNVPGVIRQVSAILAQHKYNIEGEYLRTTHELGYLVTDVNRKVTPEVLAE
                     LKRVRATLKVRVLF"
     gene            3717..>4359
                     /locus_tag="AC16GM_05665"
     CDS             3717..>4359
                     /locus_tag="AC16GM_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR2923199.1"
                     /translation="MPKFVSFNPGPSQVYDEIVADFKRAVEEHILSLSHRSEEFQEIF
                     SRAVDGIRTYFSLPTDYRIYFVSSATECMEIVLRGCCQYRSFHFVNGAFARKFFTYAQ
                     QMKKNPAQIVAEDGRGFDFGRFEILPDCDLVFLTQNETSTGVRLPYESIEKVRRAYPE
                     KIIAVDIVSSAVSERVPFEMTDCWFFSVQKGCALPAGLGVLIVSGRALEKARSL"
BASE COUNT          903 a         1229 c         1201 g         1026 t
ORIGIN      
        1 ttcctccacc gcctgcttga gtttctggtt ttcttcccgg agcaaatcgg gcataggagt
       61 gaaatatgct ggagcaaagg cgggggggca actatcagag ggcgtgttgt gcggggaggc
      121 gaattactct gagttcagta ctctcttttt gatttcctgc aaatggtgct gaaattccgg
      181 ctcatgccgc agttcttcca ggaggggatt gaattccagg aagagccaat caattttatt
      241 ggcctccatc cccttggtca tccactcata caccttaccc ttctcgccca gaagggtgta
      301 ggtcaggatg gtattttcga tcacatactc atgccaggcg aaggtcttga gcgccccgtc
      361 caatttctcc cgggcttttt cgtggtctcc caaagccgcg tagatcatgg ccagaccgga
      421 gtttccgatc ggttcggctg gttccatttc aacgtatttt tgcatctgtg ccaatgccga
      481 ctcgtactgc ctctggtaaa aggaaattcg cgccttcacg tagtggccca tcttgaggta
      541 gaaagacgac tcccccgcct ccagcagttg atcgatataa ttttccgccc gagaaaagtt
      601 attcacgctc aggtggaaat tggccaaaag gtacaggagg accgtcatcg aggggtcttg
      661 ctcgtacgcc aagaggtatt ccttctcggc ttccgcaaaa ttccccaggt tcgtgtagac
      721 gacgcccaaa ctggcccggg ccggggcgta attcggcttg atggccaagg cctggaggaa
      781 ttgctctttc gatttgcgat tgtggccttg cttggagtag atgaccccca aggccttgta
      841 cgcttccgcg cagcgggagt caatccgcag ggctttgaga cccgcctctt cggctttggc
      901 gaaaaaggtt tggtccgtct ccgagtactt gtcaatcatc tgcgcgtagc agtcggatag
      961 cccggcatgg gccagggcat actgcgggtc gagctccagc gcactgttga acatctgcat
     1021 ggcggtcagt aaatcgggct tggtgtattt gtagtagtac ccccgcccct ttaagtagca
     1081 actgtaagca tccagatcgc ctttatactt catggcgatt tgttcttcgt ccttttcgga
     1141 aagtgtgacc tggagcgcca gcgcaatttt tttggcgatc tcctcctgga gcacgaagac
     1201 gtcttgcatt tcgcggtcgt accgctccgc ccagagatgg aagccttcct tggtatcgat
     1261 gagctgggcg gtgatgcgga gccggttgcc gaacttccgc acgctcccct ccaggaccgc
     1321 atccagattc agttttctgc ccaaaagcgg gatatcgacc ggttgatcct tgtagggcaa
     1381 aacggcattg cgcgaggcca cccggagact ttcgattttc gacagatcgg tgatgatatc
     1441 ctcggtgatg ccggcggaga agtagtcgct gtccggctcg gcgctgagat tctcaaaata
     1501 gagcacggcg actgctttct tctctttttt cggggcttct ggcttgcctt cttccaattc
     1561 cttcttacat ctgcggagat cgacaaccag ttcggccgcc gactgaaaac gttcctctct
     1621 tttcttcgcc agcatttttt ccactatctc ttgcaactgc gcgggaacct caccggcaaa
     1681 gcgggccagg ggctggggcg gctcgttcaa aatggagtag aggatcgccg tttgatgttc
     1741 gcccgggaag gggagttggc cagtgaggag ttcgtagaag acaaccccca gcgaaaataa
     1801 atccgaccgc tggtcaacct cttcgccggt ggcctgctcc ggcgacatat aggcgaccgt
     1861 cccggccgtg gagccgggct ggatcagccg ggaagcgcct ttgagtttgg ccaggccaaa
     1921 gtccaagatt ttcacccgtc cgtcctgatt gatcagaatg ttgtcggact ttatatcccg
     1981 atggacgatg ccggcctggt gcgccttctc caaaccttca gcgatttgaa tggcaatgtc
     2041 cagagctttc cctgcgggca gccccctgcc ggccgccaaa tcccgcagcg attgccccgc
     2101 gacgtattcc atggcgatgt acccggcccg ttgatactcg gccacttcgt acacggtgat
     2161 gatgttggga tgattcagcg cggctgctgc ctgggcttcc tgcttgaagc gcagttttgt
     2221 ttccgggtcg gcggtgagtt gctgcggaag gaacttgagc gcaacttttc ggttcaggtc
     2281 ggtatcttcg gccaggtaga cctcccccat ccccccttcg cccaatttct ccaagatttt
     2341 gtaatgcgaa atcgtttggc cgaccatcag aaattgaata tatctttcgg gcgagggaga
     2401 cgcatctcaa gaaacctttc tgggttaccc gcagtctccg ccggggagtt gactaattcc
     2461 ctccgccgtg tcaatttggt cgggataatg gccaaaggta cccgaccagc aataaaagtc
     2521 ctgctcctgg aaaaaattaa ccccggcgcg gaggaacaat ttcgggcgca aaagtaccgc
     2581 gtcaccctac gagcgggggc gctttccgag ccggagttgg ccggcgtact tgggccggtg
     2641 caaattctgg gcatccgctc gaaaacgcag gtaactccaa aaatcctgca gcaggcagaa
     2701 aatctcctgg ccatcggctg cttctgcatc ggcactgacc aggtggacct gcgcgcctgc
     2761 accgaacggg gcattgccgt cttcaacgcc ccctactcca gcacccgcag cgtgaccgag
     2821 ttagtcatcg gcgaaatcat catgctcctg cggcgggtga ccgaaaggag taccgccgcc
     2881 catcatggac gctggctcaa aagcgacgac tccagttttg aggtgcgcgg gaagaccctg
     2941 ggcatcttcg ggtacggcca catcggctcg caggtgtcgg tcatcgccga agcgttgggc
     3001 atgaacgtca tttactacga catcctggac aaactctcct tgggcaatgc ccggtcagtg
     3061 cattccctct ccgaattact ccgcagggcc gacatcgtca ccctgcatgt gccggaggat
     3121 aaaaccacac ggcatatgat cggcgcaagg gaaatggcgc agatgaagaa ggggagttac
     3181 ctgattaaca ccagccgcgg gagcgtcgtc gtgctggaag atctctatcg cgcactggtg
     3241 gacggacacc tgcgcggtgt agcgattgac gtctatccca acgaaccctc cgggccgaac
     3301 gctcgcttca cctgtctttt gcaaggtctg ccgaacgtca ttctgacgcc ccacatcgcc
     3361 gggagcactc aagaagcgca ggccgacatt gcccagaaga cctccagccg gctgatacga
     3421 tttctggaat tcggcacgac taccggcagc atcaacttcc ccgaagtcga attgcccgtg
     3481 ctgaaccaga accaccggat tatccacatt catcggaacg tccccggggt gatcagacag
     3541 gtaagcgcaa ttctggcaca gcacaagtac aatatcgaag gcgaatacct gcgtaccacc
     3601 catgagttgg gttacctcgt aaccgacgtc aaccgaaaag taacgccaga ggttctcgca
     3661 gaacttaagc gagttcgggc caccctcaag gttcgggtat tgttctaaga cttcaaatgc
     3721 cgaagttcgt ttcctttaat cccggccctt cgcaggttta cgacgaaatc gtggccgatt
     3781 tcaagcgggc ggtggaagaa cacatccttt cgctctccca ccgttcggaa gaatttcagg
     3841 agatttttag ccgcgccgtt gacggcattc gcacttactt ctccttgccg actgactatc
     3901 gcatctactt cgtctcctca gcgaccgagt gtatggagat tgtcctgcgg ggatgctgcc
     3961 aatatcgcag ttttcacttt gtgaacgggg cctttgcccg gaagtttttt acctatgctc
     4021 agcagatgaa aaagaatccg gcgcaaattg tggctgaaga tggccgggga tttgatttcg
     4081 gacgtttcga aattctcccc gattgcgacc tggtcttcct tacccagaac gaaacctcca
     4141 ccggcgtgcg gctgccgtat gaaagtattg aaaaagttcg gcgggcatat cctgaaaaaa
     4201 tcatcgcagt agacatcgtc tcctctgccg taagcgagcg ggtgccgttt gagatgaccg
     4261 attgctggtt tttctcggtg cagaaaggat gcgccctgcc ggcggggttg ggggttttaa
     4321 tcgtttcggg gcgggcactg gagaaggcgc gatcgctgg
//
LOCUS       DBNGZD010000217         8566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1562351, whole genome shotgun sequence.
ACCESSION   DBNGZD010000217 DBNGZD010000000
VERSION     DBNGZD010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1562351"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="AC16GM_05670"
     CDS             complement(<1..807)
                     /locus_tag="AC16GM_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923200.1"
                     /translation="MRVRILLFTAALSLLATIAFAAQSSVPNLINFQGKLTDPAGNPV
                     TDGSHSANFSLWTLRTGGLSVWSEITTQTTAGGLFTHQLGSVSSLSPTLFQDYDSLFL
                     EIVADGQTITPRTKLTSVPYSRVAGGLEVGSASGFCAAPNQLAIETDASDHRISTYGC
                     DGQEQIRLWGVSWGEMLLHDQSAANVTTARLSAQPSFNPSGGGGELFLRNNPAGSVID
                     LYAGNTGDASVVLPTDAINSAEIKDEPGVANNDNTSFFNLNAGNIVYVVDS"
     gene            1157..3634
                     /gene="gyrA"
                     /locus_tag="AC16GM_05675"
     CDS             1157..3634
                     /gene="gyrA"
                     /locus_tag="AC16GM_05675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR2923201.1"
                     /translation="MPLEREKILPVFIEEEMKSSYIDYAMSVITARALPDVRDGLKPS
                     NRRISVAMNDLGLAPNRAHRKCAKIAGDTSGNYHPHGEQVVYPTLVRMAQDFNMRYPL
                     VDGQGNFGSIDGDAAAAMRYTEARLTPLAMEMLADLEKDTVDFQPNYDETRQEPKVLP
                     GKFPNLICNGSSGIAVGMATNIPPHNLSEVVDAVIAQIDNPDIDLDELLQHIKGPDFP
                     TGGIITGTRGIGEAYRTGKGRIVVRARATIERLKGDKNNIVVTEIPFQVNKSQLVERI
                     AELVREKVIEGISDLRDESDRDGMRIVVELKRDVVPEVVLNQLFKHTQMQETFGIINL
                     ALADGVPQVLPLKAILQNFIDHRHVVVTRRTQFDLKKAEERAHILEGYKIALDNLDAV
                     ITLIRKSQTPEEAREGLMKRFKLSQIQAQAILDLRLQRLTGLERKKIEEEYLETIKLI
                     EELKSILASKTKRLAIIKEELSELKQKYGDERRTEIREEEEEFSIEDLIAEEDMVITI
                     THSGYIKRLAVTAYRRQGRGGRGVIGIETKEEDFVEHLFIASTHEYLLFFTRAGRLYW
                     LKVHEIPTGGRLSKGKPIVNLLALSENEKIAAMVPVRRFDDQHFIVMATRNGVIKKTS
                     LDEFSNPRKGGIIAVTIPENEKDELIDVAVTDGTCDIVLATRQGQAIRFPEDKVRSMG
                     RTAYGVIGIELEKKDYLIGMVVLKRKATLLVVTEKGYGKRSDIDDYRVTNRGGKGVIN
                     VKVTDRVGEVIAIKEVLDSDELMIITQKGIVIRLPIKPIRVMGRNTQGVRLINLEEGD
                     KVTDVARVVTEGEETEQVVEGDGQAKA"
     gene            3729..4229
                     /locus_tag="AC16GM_05680"
     CDS             3729..4229
                     /locus_tag="AC16GM_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2923202.1"
                     /translation="MPQRKTRSKGTAKKAPAKTGKSIARTITQNCFIHAYPEVVFDAL
                     VNPLVLKRWFVEEAEVEPKKGGRYVWDWGKGGKLESKITEYQPPKKLVITFTGWNSGM
                     KEKCTFTLKPKEGGTLLHMEDTDHIAGSKGWERHISVVRGWAVALLNLKSVLESGKDL
                     RNAAAW"
     gene            4316..5311
                     /locus_tag="AC16GM_05685"
     CDS             4316..5311
                     /locus_tag="AC16GM_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2923203.1"
                     /translation="MTLQGKKVLVTGAGGFIGSHLVERLVALGARVACFLRYNSRGLK
                     GWIDEFPADVQSQLTIFWGDLKDPEAVRLAVKGQEIIFHLGALIAIPFSYITPRDFVD
                     TNVLGTTNLLNAIREYGCERLIHTSTSEVYGTAQYVPIDEKHPLVGQSPYAASKIAAD
                     MLVQSYFRSFDLPAVTIRPFNAFGPRQSLRAIVPTIITQALAGKVVSLGSVHPTRDMN
                     YVADTVEGFIAIASCEGIEGETINIGSGQEVAIRNLVERVGKILGKTLEIKTEEKRVR
                     PEASEVARLAADNRKAKKLLGWQPKVSLDAGLNKTIEWFRAKRNLFATQVREYQV"
     gene            5308..6012
                     /locus_tag="AC16GM_05690"
     CDS             5308..6012
                     /locus_tag="AC16GM_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2923204.1"
                     /translation="MRAVIMAGGKGTRLAPYTTVLPKPLLPIDSTPILEIILRQLKRA
                     GITRITLAVGHLGHLLENFFGDGQALGLSIDYHREKKPLGTAGPLVRIGSLDETFLVM
                     NGDILTDLDYRKLVAYHRRHKALATIGSYERKVPVDFGVIETNSSARLQGYREKPVLA
                     YDVSMGVYVFEPEVLKFIPKYRKFDFPELVWALLQRRKKVISYPYGGIWYDIGRPDDY
                     ARATAEFLRHRKRFFV"
     gene            6191..7762
                     /locus_tag="AC16GM_05695"
     CDS             6191..7762
                     /locus_tag="AC16GM_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923205.1"
                     /translation="MKLESPSQRKVFYLFAFGFLIRIALAWLPEKYFYFLISDDAYYY
                     FTIARNFVTRGMFSADGITLTNGFHPLWLFIISPIYLVFRGSPWLSIHFAMTVAAIFD
                     TGAGFFVYKSLEKLGKPTVGFWAACFYLVNPYGLLHTMNGLETAQNNFFLTFLVYLSV
                     RATPDWLQTRWFFLGTICGLTLLSRTDNLFAVGVLLGYLFWRNRNFTVIAKATAAAGL
                     LFAPWLIFNAVKFGSIVQTSGAAYPWLYHQQYLSEHHTYYSWALVPYLLKLGFYNFVQ
                     NAFHYGNWILTAIVVGFLIFRLRNQSQKYRPLVWTLATACVFIGVHTFIRWSVRPWYP
                     QAAFVLTLPLVALALEKVRRSVIGPGLTIVLFLSGWAVWSQPFRIAYRSIFMEDIVNR
                     IPAGERVGAFNSGYLQYFTDRKVINLDGLVNNEVLPYYRENKGLEYFRNRNIGWLVDR
                     AGYFVHVFGPYFGPAAESSLAVVASSQDTVYTGQYILTIRVLPEGMRPPPERVLQRFY
                     RPAPITWGPVPFLKP"
     gene            8084..>8566
                     /gene="pth"
                     /locus_tag="AC16GM_05700"
     CDS             8084..>8566
                     /gene="pth"
                     /locus_tag="AC16GM_05700"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR2923206.1"
                     /translation="MVKLIFGLGNPGRNYNGTRHNLGFEVIDQLAAKYKVPVAPGPGD
                     FLWGTWVRANGDVVLAKPTTFMNACGEALVQMKEFFDYTPDEVLVICDDIDLPLGKLR
                     LRPDGSDGGHKGLQSVIYHLQSEDFPRLRLGIGRPPGQMDPSAYVLQRFTRAEQDVLD
                     Q"
BASE COUNT         1901 a         2252 c         2516 g         1897 t
ORIGIN      
        1 agaatccacg acgtaaacga tattgccggc gttcaggttg aaaaagctgg tgttatcatt
       61 attcgccacc cccggctcgt ccttgatctc tgccgagttg atggcgtcgg tcggaagcac
      121 caccgacgca tctcctgtat tccccgcata caggtcgatg accgaacccg ccggattgtt
      181 gcgcaggaac aattcgcctc cgccgccgct cgggttgaag gatggttgag ccgaaagccg
      241 ggccgtagtc acgttggccg cgctttggtc gtgcaaaagc atttctcccc acgaaactcc
      301 ccagagacgg atttgctcct gcccgtcgca gccgtaggtg gaaattcggt ggtcggacgc
      361 atcggtttcg atggcgagtt gattgggagc agcgcagaat ccgctggcgc tacccacttc
      421 cagcccgccc gctacgcggc tgtagggaac gctcgttagc ttggtcctgg gagttatggt
      481 ttggccgtca gccacgattt ccagaaacag tgaatcgtag tcttgaaaga gcgtcggtga
      541 aagggagctc acggaaccca actggtgggt gaacagtccg cccgcggtgg tctgggttgt
      601 gatttcgctc cagaccgaaa gtccacctgt gcgcagcgtc cagagggaaa aattggcgct
      661 gtgggagccg tcagtgaccg ggttaccggc gggatcggtc agtttccctt ggaaattgat
      721 cagattgggg acagaagact gggcagcaaa ggcgattgtg gccaaaagag acagtgccgc
      781 agtgaaaagc aaaatccgta cgcgcattct ccacctccat ttgtgtgtac cgcgttgaac
      841 catgggcggg tcgttggcga cccttgggac tggtgaacgt gctccgctcg aatgcggatt
      901 attctgagcc cttacgctga aactctgcgg tcgataaccc tcctcttcgt tgtgcgttcc
      961 ctcttcaata ccctccgatg tactttagcg gaaagaaatt gcggaggacg ggacatgtca
     1021 aggactttct ggcccgcaca taagtaatta tgataaagaa tatccggcta cgcatctggc
     1081 atttctgggc tcactcaagt atattctctg accgggattg atggctgcca ctaacttgaa
     1141 agataaagga taacttatgc cgctggaacg cgagaaaata cttcccgttt tcattgaaga
     1201 ggagatgaag tcgtcgtaca tcgattacgc gatgtcggtg attacggcgc gggccttgcc
     1261 cgacgtgcgc gacgggctta agccctccaa ccgccgcatc tcggtggcaa tgaacgactt
     1321 ggggctggcg cccaaccgcg cccaccggaa gtgtgccaag attgcgggcg acacctccgg
     1381 gaactaccac ccccacggcg aacaggtggt ctatcccact ctcgttcgga tggcgcagga
     1441 cttcaacatg cgctacccgt tggtggacgg ccaggggaac ttcggttcca tcgacggcga
     1501 tgcggctgcg gcgatgcggt acaccgaggc gcggttgacg cctctggcaa tggagatgct
     1561 ggccgacttg gaaaaagaca ccgtcgattt ccagcccaac tacgacgaaa cccgccagga
     1621 gccgaaagtc ctccccggca aattcccgaa cttaatctgc aacggctcct ccggcatcgc
     1681 cgtcggcatg gcgaccaaca tccccccaca caacctttcc gaggtggtgg acgccgtcat
     1741 cgcgcaaatt gacaaccccg acatagacct cgatgaattg ttgcagcata tcaaagggcc
     1801 cgatttcccc accggcggga tcatcaccgg cacgcgcggc atcggcgagg cctatcgcac
     1861 cggcaaaggg cggattgtgg tccgcgcccg ggccactatc gagcgcctga aaggcgacaa
     1921 gaacaacatc gtggtcaccg aaattccgtt ccaggtgaat aaatcgcagt tggtcgaacg
     1981 gatcgccgaa ctggtccggg aaaaggtgat cgaaggcatc tccgacctgc gcgacgaatc
     2041 ggaccgggac gggatgcgga tcgtggtcga actgaagcgc gacgtggtgc cggaagtggt
     2101 gctgaaccag ctcttcaagc acactcagat gcaggagacc ttcgggatca tcaatctcgc
     2161 cctggcggac ggcgtcccgc aggtgctccc gctcaaggca attttgcaaa acttcatcga
     2221 ccaccggcac gtggtggtta cccgccggac ccagttcgac ttaaagaaag ccgaagaacg
     2281 ggcacacatt ctcgaagggt acaagattgc tctggataat ctggacgcgg tcatcacctt
     2341 aatccgcaaa tcgcagacgc cggaggaagc gagggaaggt ttgatgaagc gcttcaaact
     2401 ttcccagatc caggcgcagg cgatcctcga tctgcgtttg cagcggctca ccggtttaga
     2461 gcgcaaaaaa attgaagaag aatatctcga aacgatcaag ttaatcgagg aattgaagag
     2521 cattctggcc tccaagacca agcggctggc aatcattaaa gaggaacttt ccgaactcaa
     2581 gcaaaaatac ggcgacgagc gccggaccga aatccgcgag gaagaggaag aattttccat
     2641 cgaagaccta atcgccgaag aggacatggt gatcaccatc actcactccg gctacatcaa
     2701 aaggctggcg gtgacggcct accggcggca ggggcggggc gggcgcgggg tgatcggcat
     2761 cgagaccaaa gaggaggatt tcgtcgagca cctcttcatc gcctccaccc acgagtacct
     2821 cctctttttc acgcgggcgg ggcggctcta ctggctcaaa gtgcacgaaa ttcccaccgg
     2881 cgggcggctc tccaaaggga aaccgatcgt caacctcctg gctttgagtg aaaacgaaaa
     2941 aatcgccgcc atggtgccgg tgcggcggtt cgacgaccag catttcattg tcatggccac
     3001 ccgcaacgga gtgatcaaaa agacttcgct ggacgaattc tccaacccgc gcaaaggcgg
     3061 catcatcgcc gtcaccattc ccgaaaacga aaaagacgag ttgatcgacg tggcggtcac
     3121 cgacggcacc tgcgacatcg ttttggccac ccggcagggg caggcgatcc gttttcccga
     3181 agacaaagtg cgctccatgg gccgcaccgc ctacggcgtc atcggcatcg aattggagaa
     3241 gaaagactac ctcatcggca tggtcgtgct caagcgcaag gcaacgcttt tggtcgttac
     3301 cgaaaaaggg tacggcaagc gctccgacat tgacgactac cgggtgacca accggggcgg
     3361 caaaggggtg atcaacgtca aggttaccga ccgggtgggc gaagtgatag ccatcaaaga
     3421 agtgctcgac tccgacgaat tgatgatcat cacccaaaaa gggatcgtca tccggctgcc
     3481 gattaagccg attcgggtga tgggccggaa cacccagggg gtgcgactga tcaacctcga
     3541 agaaggcgac aaggtgaccg acgtggcgcg ggtagtgacc gaaggcgaag aaaccgagca
     3601 agtggtggag ggcgacggcc aggcgaaggc ctgacaacgg atttttgaac ggattaaacg
     3661 gattgacttt ggtagcatag ccctttgtgg gcatctggat cttttttaga atattcgttg
     3721 agacattcat gccccagagg aaaactcgat ctaaagggac ggcgaagaaa gctccagcaa
     3781 aaacgggaaa atcaattgcc cgcaccatca cccaaaactg cttcatccac gcctacccgg
     3841 aagtggtgtt cgacgcgctg gtaaaccctc tggtgctcaa gcggtggttt gtggaggagg
     3901 cagaggtcga gccgaaaaag ggcgggcggt acgtctggga ctggggtaag ggtggcaaat
     3961 tagaaagcaa aatcacagaa taccagccgc ccaaaaaact cgtcatcacc ttcaccggct
     4021 ggaatagcgg gatgaaggaa aaatgcactt ttactctcaa gcctaaagag ggcgggacgc
     4081 tcctgcatat ggaagatacc gaccacatcg ccgggtccaa aggttgggaa cggcacatct
     4141 ccgtggtgcg cgggtgggcg gttgcgcttt tgaacttgaa gtcggtttta gaatcgggca
     4201 aagacctgcg caatgcggcc gcctggtaag acccgctaat agtcggccgt aatctttgag
     4261 ggggcgcgtt tcgattcaac tcgattctac cgataattag accaggatga gtgggatcac
     4321 cctgcagggc aaaaaggtgc tcgtcacggg cgccggcggg ttcatcggca gccatttagt
     4381 cgagcggctg gtggctctcg gagcgcgggt tgcctgtttt ctgcgctaca actcgcgcgg
     4441 gctcaaaggg tggattgatg aattccccgc cgacgtgcaa agtcagctca ccatcttctg
     4501 gggcgacctg aaagacccgg aggcggtccg gctggcggtc aagggacagg agatcatctt
     4561 ccacctcggt gctttgattg ccattccttt ttcctatatc acgccgcgcg acttcgtgga
     4621 tacgaacgtg ctgggaacga cgaacctgct caatgcaatt agagaatatg gctgtgagcg
     4681 gttgattcac acttccacga gcgaagtgta cggcacggcg cagtacgttc ccatcgatga
     4741 aaagcatcct ttggtgggac aatcgcccta cgcggcctcg aaaattgcgg cggatatgct
     4801 cgtgcagagc tactttcgtt ctttcgacct gccggcggtg accattcgtc cgttcaatgc
     4861 tttcgggccg cgccagtcgt tgcgggcaat tgtgccgacc atcatcacgc aggcgttggc
     4921 cggcaaagtc gtttcgctgg ggtcggttca tccgacgcgg gatatgaatt acgtcgccga
     4981 tactgtcgag ggtttcattg cgatcgcatc ttgcgaaggc atcgagggcg agacaatcaa
     5041 catcggctcc gggcaggagg ttgcgattcg gaatttagtg gagcgagtcg ggaaaattct
     5101 cggtaagact ttggagataa aaaccgaaga gaagcgggtg cggccggagg cgagcgaggt
     5161 cgcgcggctg gcggcggata atcggaaagc gaaaaaactt ttggggtggc agccgaaggt
     5221 ctcccttgac gcgggactga acaaaacaat cgagtggttc cgcgcgaagc ggaatctctt
     5281 tgcaacgcag gtgcgcgagt accaggtatg agggcggtca tcatggcggg cgggaagggg
     5341 acgcggctgg ctccgtacac gaccgttttg cccaagccgc tcttgccgat tgattcgacg
     5401 ccgattttag aaatcatcct ccggcagtta aagcgggcag gcatcacgcg cattacgctc
     5461 gcggtcgggc acctggggca tttgctggag aatttttttg gagatgggca ggcgctggga
     5521 ctctcgattg actaccaccg cgaaaaaaag cctttgggga cggcggggcc gctggtgcga
     5581 attggtagtc ttgacgaaac gtttctggtg atgaatggcg acatcctgac ggatctcgac
     5641 taccggaaat tggtcgcgta ccaccggcga cacaaagcgc tggcgaccat cggatcgtac
     5701 gagcgaaaag tgccggttga tttcggagtg attgagacga atagcagcgc tcgtttacaa
     5761 ggataccggg aaaagccggt gctcgcgtac gacgtcagca tgggcgttta tgtttttgaa
     5821 ccggaagtgc tcaagttcat cccgaagtac cgaaaattcg atttcccgga gttagtgtgg
     5881 gcgcttttgc agcggaggaa gaaagtgatt tcatacccgt acgggggcat ctggtatgac
     5941 atcgggcggc cggatgacta cgcgcgggcg acggcggagt ttttgagaca tcggaagcgg
     6001 ttttttgtat agataaagtc gtaggtggaa gatggtgggg gactaaagaa gccccccgaa
     6061 gttccggggt tgatgcggct cgttccggga aggggcgcgg gggaatggag cgggggtcag
     6121 ttgcaaaagc ataaaacata ttctcctact atttcgcaca ctttaatttc attctgttta
     6181 tattgaatta gtgaagcttg agagcccgtc tcaaagaaag gttttttatc tttttgcttt
     6241 tggctttctc atccggatcg ctctcgcctg gttgcccgag aagtatttct atttcctcat
     6301 ttctgacgat gcctactact actttaccat agccagaaat tttgttacgc ggggaatgtt
     6361 ttcagcggac ggcattaccc tcaccaacgg gttccatcct ctctggttat ttatcatctc
     6421 gccgatttac ctcgtttttc gaggcagtcc gtggctcagc attcattttg cgatgaccgt
     6481 agcagcgatt tttgataccg gcgccggttt ctttgtctac aagagtctgg aaaaactggg
     6541 gaaaccaacc gttggctttt gggccgcgtg tttctatctg gtcaatccct atggtttgtt
     6601 gcacaccatg aatggcctgg aaaccgctca gaacaatttt tttctgactt ttctggtgta
     6661 cctgtctgtg cgcgctacac ctgattggct ccaaacgcgc tggtttttct tgggaaccat
     6721 ctgcggtttg acgttacttt cccgcaccga taacttattc gccgttggcg ttctccttgg
     6781 ctatcttttt tggcgcaatc ggaacttcac cgtgattgcg aaagccactg ccgcagcggg
     6841 cctgcttttt gcgccttggt taatttttaa cgcggtgaag ttcggttcaa tcgtgcagac
     6901 ctcaggtgcc gcgtacccct ggctttacca ccagcagtat ctcagtgagc atcacacgta
     6961 ctattcttgg gcgctggttc cttacttatt gaagttgggt ttttacaatt tcgtccagaa
     7021 cgccttccat tacggcaact ggatactcac ggctattgtg gttgggtttc tcatctttcg
     7081 gctcaggaac cagtcgcaga aataccggcc actggtgtgg accctggcaa ctgcgtgtgt
     7141 ttttattgga gttcacacct tcatccgctg gtcagttcgt ccctggtatc cgcaagccgc
     7201 ttttgtgctg actctccccc tggtggccct ggcactggaa aaagtcaggc ggtcggtgat
     7261 tggtcccggg ctcaccatcg tactctttct cagcgggtgg gcggtatggt cgcagccatt
     7321 tcgaattgcc taccgctcga ttttcatgga ggacattgtc aaccggattc cggccgggga
     7381 gcgcgtcggg gctttcaact ccggttacct gcagtacttc accgaccgaa aagtgataaa
     7441 cttagacggc ctggtgaata atgaggtttt accatactat agagagaaca aggggttgga
     7501 atatttccgt aaccgaaata ttgggtggct ggtggataga gcggggtact tcgtccacgt
     7561 attcggcccc tattttggtc cggcagccga aagctcactg gccgtcgtgg cgtccagtca
     7621 agataccgtt tatacgggtc aatacatcct tacgattcga gttctgcctg aggggatgcg
     7681 gcccccgccg gaaagggtgc tccagagatt ctaccggcca gctccaatca cgtgggggcc
     7741 ggttccattc ctcaagccgt aatcgcggcg gggctgtact agtcacggtt atttttgtca
     7801 actttgccgt ttaggatagc tctctggtcc cccgactttg ttcggggttc tttgtattca
     7861 ggccaggcgc ccctaacaac aatgtattcg ccttcgctca ctacgggtgt gcgccaccgg
     7921 cgggtggtgt tcccgggaag agaggacgaa tccgcctcac gcggatttgc ctttacaggt
     7981 ttgcggacgg ggaacttgac aaaagagcgt cggcgcatat actggcctgt ggcaagccgc
     8041 aggattagtt ctccggcggc ttcttttgat ataattacga tgcatggtca agctgatttt
     8101 cgggttaggg aatccgggcc gcaattacaa cggcacgcgg cacaacttgg ggtttgaggt
     8161 gattgatcaa ttagcggcga aatacaaagt tccggtagca cccgggccgg gagatttttt
     8221 gtggggtacg tgggtgaggg caaacggcga cgtcgtcttg gccaagccga cgacattcat
     8281 gaatgcctgc ggcgaggcgc tggtgcagat gaaagagttt ttcgattata cgcccgacga
     8341 agttttagtc atctgcgatg acattgattt gccgttaggc aaactgcgtt tgcgtccgga
     8401 tggttcggac ggcgggcata aggggttgca atcggtaatc taccacctgc agtcggagga
     8461 ttttccccgc ctgcgcctgg ggatcggccg cccgccgggc cagatggacc cttccgccta
     8521 tgtgctgcag cgcttcactc gcgccgaaca ggatgtgctg gaccag
//
LOCUS       DBNGZD010000218         5558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1909590, whole genome shotgun sequence.
ACCESSION   DBNGZD010000218 DBNGZD010000000
VERSION     DBNGZD010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1909590"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..714)
                     /locus_tag="AC16GM_05705"
     CDS             complement(124..714)
                     /locus_tag="AC16GM_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2923207.1"
                     /translation="MRKIILFVTGAVLLLTIPAFAQYAGKSAEKGKWSVAPFGNVSFP
                     MGSLKDFTKTGFGGGLAAEYYVADAFAVGVQGSYNTFSGDPSPLPDWRPWQYGAFGKY
                     VIPTQSKLSPWIKYGAGGYSLTFSKVAGATTTAPSQSRVGWFGGLGVAIEATPTVSVF
                     VEGLYHDALKKHELPVNWVSAGVGVQFLLGIGSTAK"
     gene            complement(909..1592)
                     /locus_tag="AC16GM_05710"
     CDS             complement(909..1592)
                     /locus_tag="AC16GM_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923208.1"
                     /translation="MRAKAKTKVWPRVVVGTTLALLGAAGAVHGQEAKGKGAVVVGFG
                     TGPALPIGLSNTPNVPDDFPDAYGSGFGGSASLSYYVHDRIEVGLAAGLNWHHPTDGF
                     DQLYQLSGNTAAPDVSIIPVVGFIKTDLAPQKKLTPYVRVGGGLYRLQFGSVTTAGGA
                     FKSVAAVNKSGVFAAGGFQLDLVPAVQVFLEGAFHNIFKFDFFNVDPSKSFGLVTVQG
                     GLRLGLGRP"
     gene            complement(1657..2304)
                     /locus_tag="AC16GM_05715"
     CDS             complement(1657..2304)
                     /locus_tag="AC16GM_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2923209.1"
                     /translation="MRRLVVVCLLAGIVGLGWTGAGGAELKGKVTIAPALGLLTPVGD
                     FNDQMDMGFTGGAQVEYYFSNLVGFGLTASGNFFPGVARPTGILPSGLRDLPDPDTTL
                     AQFGIFASLHLRPAQRLSPYFKGGWTMNVIFESNSFEDQEGARIDTVTTHHTETDFGV
                     FGGAGLSYDLTERTSFFTEAVVHDLFRLDETINEFTGRQYVQVWVGARFTFDWFK"
     gene            complement(2351..4438)
                     /gene="fusA"
                     /locus_tag="AC16GM_05720"
     CDS             complement(2351..4438)
                     /gene="fusA"
                     /locus_tag="AC16GM_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR2923210.1"
                     /translation="MREYTLEQIRNVAFVGHGGSGKTSLAEAALFAAKVTTRLGKVDD
                     GTTISDYSPDEITRKISISSTLLHFDWNGTKFNVVDLPGYTDFIGEVHGGLRVCETAV
                     VAVNAASGVEVGTEQVWQLGDRYGTSRVIFVNKIDKEHADFAKVLAVLQERFGHLVVP
                     VQLPLGSGPGFGGVIDLLQNKAFRFTGGTAKAEEIPAEFKNAAADARQKLLEAAAEAD
                     DSLLEQFFEAGELTDVQIQYGLKKGLAAKKIFPVLVGAATASAGTSLLVDFLANFAPS
                     PVDVPEVKGILSGTEQEVVRKTLPSEPVCALIFKTISEPHVGELSFFRVFSGHVKTGD
                     DIFNATRQHSERVGQIYAMNGKERKEVGTVSAGDIGAFVKLKNTHTGDTLCDRKAPIL
                     LPPVDFPKPVINMAIKPKAKTDDEKIASGLARLHEEDPTFSVEVNSDIKQTIVHGQGE
                     LHLEVIIDRLKRKYGVEVELEKPRIPYRETIKSKTEVQGKYKKQTGGHGQYGDCWLRI
                     EPLKRGTGFEFEDEIVGGAVPGKYIPSVEKGVVEAMQEGGLAGYRVVDVKVTIFDGSY
                     HTVDSSDIAFKVAGSMAFRSGFLQCKPVLLEPIYHVAVTVPDEYMGDVMGDLSARRGK
                     ILGMERNGASQTIKALVPLADLYKYSTALRSLTQGRGLHRREFSHYEEVPREIAEKII
                     KEAQETKAHEKEH"
     gene            4569..5513
                     /gene="argF"
                     /locus_tag="AC16GM_05725"
     CDS             4569..5513
                     /gene="argF"
                     /locus_tag="AC16GM_05725"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR2923211.1"
                     /translation="MPPASNTSRRGKRDFLTVTDFSAREISETLMLAQSLKKGKTRPQ
                     PLKGKSVACIFHKPSLRTRVSFETGIFQLGGHPIYITEKEIELGRRETIADAARVFSR
                     YVALIVIRTFAQKDVEELAKYATVPVINALTDLSHPCQIMGDLFTIWERRKNLKALSV
                     AYLGDGNNVANSWLELAARIPLDLRIGTSPQTMPDEETFRRAQETGISKIRITADPFS
                     AVQGVDVVYTDVWASMGQKHLAEEKAQFLSRFQVNSALLKHAKANALVMHCLPAKRGQ
                     EITDEVMDGPQSVVFDQAENRLHIQKAIMVKLLHGSKM"
BASE COUNT         1092 a         1764 c         1500 g         1202 t
ORIGIN      
        1 aaaaccggca acacttttct cacgttctgc ctcccgtcct gtttccgagg ccatctgccc
       61 ctcggagatc tgtcaaagag agaacgccgt gcccctactg gggcacggcg tccgtgaaca
      121 ccgctacttg gccgtacttc caatcccgag caagaactgg acgcctactc cggcactgac
      181 ccagttaacg ggaagctcgt gcttcttgag ggcatcgtgg tagagaccct cgacaaagac
      241 cgagaccgtc ggagtggctt caatcgccac gccgagcccg ccgaaccatc cgacgcggga
      301 ttgactcggc gcagtggtag tggccccggc gacttttgag aaggtgaggc tgtagccgcc
      361 cgcaccgtac ttaatccagg gcgagagttt cgactgggtt gggatcacgt acttcccgaa
      421 agcgccgtac tgccagggac gccaatccgg gagggggctg ggatcgccgg aaaaggtgtt
      481 gtaactcccc tggacaccga ccgcaaaggc gtcggcgacg tagtattccg ccgccagccc
      541 gccgccgaat cctgttttcg tgaagtcttt cagtgatccc atgggaaaac tgacgttgcc
      601 gaagggcgcc accgaccatt tccctttctc cgccgacttg cctgcgtact gcgcgaaggc
      661 cggaatggtc aggagcagca ccgctcccgt cacgaacaaa atgatcttcc gcatcttact
      721 cctcctgttt ggtttacctc ttcgactccg attacagata ccaaaccgct gctaatcagt
      781 cttttacctc acctccttgt tcactcccgg gtttactccc cggggcttcg accgctggtt
      841 ctatggcaat ctctagtaaa catttccaca tacggctacc cgcaaaaaat gttgaggagt
      901 tttttatgtc agggtctccc caatccgagc cgcagtccgc cctgcacggt gacgaggcca
      961 aaagacttgc tcgggtcaac gttaaagaaa tcgaacttga agatgttgtg gaaggcacct
     1021 tcgaggaaga cctgcaccgc cggaaccaga tccagctgaa acccgccggc ggcgaatacg
     1081 ccgcttttgt tcaccgctgc gacggacttg aacgctccgc ccgcggtggt gacgctgccg
     1141 aactgcagcc ggtagaggcc gccgcccacc cgcacgtagg gcgtcaattt tttttgcggg
     1201 gctaagtcgg tcttgataaa gcccacgacg gggatgatgc tcacgtccgg cgcggcggta
     1261 ttcccgctca gctggtagag ctggtcaaag ccgtcggtgg gatggtgcca attgagaccg
     1321 gcggccaggc cgacctcgat ccggtcatgc acgtagtagg aaaggctggc gctcccgccg
     1381 aagccgctcc cgtaggcatc ggggaaatcg tcgggaacgt tgggcgtatt cgaaaggccg
     1441 atgggcagag ccggaccggt tccgaagccg accaccaccg cccccttccc tttggcctcc
     1501 tgtccgtgga ccgcaccagc cgctccgagg agggccaggg tcgtcccgac caccacccgc
     1561 ggccacactt ttgttttcgc cttggctctc atggctgctc catcttctga attgtatcgg
     1621 acattctctt ccgctgctcg actgccctgt tcccgctcat ttgaaccaat cgaacgtgaa
     1681 ccgggcgccg acccagacct gcacgtactg ccgtccggtg aactcgttta tggtttcatc
     1741 caggcggaag aggtcatgga ccaccgcttc cgtaaaaaaa ctggtgcgtt cggtcaggtc
     1801 gtaggagagg cccgcaccgc cgaatacccc aaaatcggtc tccgtgtggt gagtggtgac
     1861 ggtatcaatc cgggcccctt cctgatcctc aaaactgtta ctctcgaaga ttacgttcat
     1921 cgtccagccg cccttgaagt agggagagag ccgctgcgcc gggcgcaggt gcaggctggc
     1981 gaagatcccg aactgcgcga gggtcgtatc gggatcgggc aggtcgcgca ggccgctggg
     2041 caaaatgccg gtgggcctgg ccacccccgg gaaaaaattc cccgacgccg tcaggccgaa
     2101 gccgaccagg ttggaaaagt agtattcgac ctgcgccccg ccggtaaaac ccatatccat
     2161 ttgatcgttg aaatctccca ccggagtcaa tagccccaat gccggcgcaa tagttacttt
     2221 ccctttcaac tcggcaccgc cggctccggt ccagcccagc ccgacgatac ctgccaaaag
     2281 acaaaccacc accagtctac gcattgctcc tcctcgcgtt gaattcctgt tcatcgcctg
     2341 ccctttcccg tcagtgctcc ttttcgtgtg ccttggtctc ctgcgcttct ttgatgattt
     2401 tctccgcaat ctcccggggt acttcttcgt agtgcgaaaa ttcccgccgg tgcaggccgc
     2461 gcccctgggt gagtgaacgc aaagccgtcg agtacttgta gagatccgct aagggcacca
     2521 gcgccttgat ggtctgggaa gctccgttcc gctccatccc caaaattttc ccccgccgcg
     2581 ccgagagatc gcccatcacg tcgcccatgt attcgtcggg gacggtcacc gccacgtggt
     2641 aaatcggttc caaaagcacc ggtttgcact gcaggaaccc cgaccggaag gccatcgacc
     2701 cggccacctt gaaggcgatg tccgaggaat cgaccgtatg gtacgacccg tcaaagatcg
     2761 tcaccttcac gtcgaccacc cggtagccgg ccagtccccc ttcctgcatc gcttccacca
     2821 ctcccttttc caccgatgga atatacttcc cgggcaccgc gccgcctacg atttcatcct
     2881 cgaactcaaa gccggtgccg cgtttcagcg gctcgatccg gagccagcag tccccgtact
     2941 gtccgtggcc gccggtctgc ttcttgtatt tcccctgcac ttcggttttg ctcttgatcg
     3001 tctcccggta cggaatgcgc ggtttttcca gttcgacttc cacgccgtac ttgcgcttga
     3061 gccggtcgat gatcacttct aagtgcagtt ccccctgtcc gtgaacgatc gtctgcttga
     3121 tgtcgctgtt cacttccacc gaaaaggtgg gatcttcctc atgcaggcgc gccaggccgg
     3181 aggcgatttt ttcgtcgtcg gttttcgctt tgggtttgat cgccatattg atgaccggtt
     3241 tgggaaaatc gaccggcggc aaaaggatcg gcgccttgcg gtcgcagagg gtgtcgcccg
     3301 tgtgggtgtt tttcagtttc acgaacgcac cgatgtcgcc cgccgacacg gtgccgactt
     3361 ctttgcgctc cttgccgttc atggcgtaaa tctggcccac ccgttcgctg tgctggcggg
     3421 tggcgttgaa aatgtcgtcg ccggttttca cgtggccgga gaagacccgg aaaaacgaca
     3481 gttcccccac gtgcggctcg gaaatcgttt tgaaaatcaa ggcgcagacc ggctcgctgg
     3541 gcagagtttt gcggacgact tcctgttccg tgccggataa aattcccttg acttcgggca
     3601 cgtccaccgg gctcggagca aagttggcca ggaaatcgac caaaaggctc gtacccgccg
     3661 aagccgtcgc cgcgccgact aaaaccggaa aaatcttctt agccgccaga cccttcttca
     3721 acccgtactg aatttgcacg tcggtcagtt cgccggcttc aaagaattgc tctaagagcg
     3781 aatcgtccgc ttccgcagcc gcttcgagga gtttctgtcg cgcgtcggcc gctgcatttt
     3841 tgaactcggc gggaatttcc tccgctttgg ccgttccgcc ggtgaaccgg aaggctttgt
     3901 tctgcaggag gtcgattact ccgccaaatc ccggcccgct gcccaaaggc aattgcaccg
     3961 gcacgacgag atgcccgaac cgctcctgca aaaccgctaa cactttggcg aaatcggcat
     4021 gctccttgtc aattttgttg acgaagatga cccggctcgt gccgtagcgg tcgcccaact
     4081 gccagacctg ctcggtgccc acttccacgc cggaggcggc attcaccgcc accaccgcgg
     4141 tctcgcagac ccggagccca ccgtgcactt ccccgatgaa atcggtgtac cccggcaggt
     4201 ccacgacgtt gaacttggtc ccgttccaat cgaagtggag cagggtggaa ctgatcgaaa
     4261 tcttccgcgt gatttcgtcc ggactgtagt cggaaatggt cgtcccgtcg tcgacttttc
     4321 ccaaacgcgt ggtaaccttg gcggcaaata aggcggcctc ggccagcgag gtcttccccg
     4381 acccgccgtg gcctacgaaa gcgacgttcc gaatctgctc aagcgtgtac tcccgcatcg
     4441 ctgcaacacc ccggataaaa cttgcctaac ctacgtttcg tgcaggactt tgtcaactgt
     4501 ccggtaattg aagacccgga tcctccgctg cttgacaccg ccctccccgt ctctacatta
     4561 gccgcacgat gccccctgcc tcaaatacct cccggagagg aaaacgcgac ttcctcaccg
     4621 tcaccgactt ttccgcccgt gagattagcg agacattgat gttagcccag tcgcttaaaa
     4681 agggcaagac tcgtccccag cccttgaaag ggaagagtgt cgcctgcatc ttccacaaac
     4741 cgtcgttgcg cacccgggtc agttttgaga cgggaatctt ccagttgggc ggccatccga
     4801 tttacattac ggaaaaagag attgaactcg gccggcgcga gacgattgcc gacgccgccc
     4861 gcgtcttctc ccgctatgta gcgttgattg tgattcgcac cttcgcgcag aaagatgtcg
     4921 aggaactggc caaatacgcg acggtccctg tcatcaatgc cttgaccgac ctttcccacc
     4981 cctgccagat tatgggcgac ctcttcacca tttgggagcg ccgaaagaat ttgaaagcgc
     5041 tttcggtcgc ttatctcggt gacggaaaca atgtagcgaa ttcctggttg gaattggcgg
     5101 cccgaattcc gctcgattta cggatcggca cctccccgca gacgatgccg gatgaggaaa
     5161 cgttccggcg agcacaggag accgggattt ccaagatacg tatcacggct gaccctttct
     5221 ccgccgttca gggtgtcgat gtggtctaca ccgacgtctg ggccagcatg ggccagaagc
     5281 atttagccga agaaaaagcc caattcctct cgcgctttca ggtgaattcc gccctgctca
     5341 agcacgccaa agcgaatgct ttggtcatgc actgcctgcc ggccaagcgc ggtcaggaga
     5401 ttaccgatga ggtgatggac ggcccgcagt cggtcgtctt cgatcaggct gaaaaccgcc
     5461 tccacatcca aaaggcgatt atggtgaaat tattgcacgg ctcgaagatg tgatttgttt
     5521 tctggctact ggctacttga ccctggctac ttttttcc
//
LOCUS       DBNGZD010000219         9842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_34356, whole genome shotgun sequence.
ACCESSION   DBNGZD010000219 DBNGZD010000000
VERSION     DBNGZD010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_34356"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="AC16GM_05730"
     CDS             <1..723
                     /locus_tag="AC16GM_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR2923212.1"
                     /translation="TETPTNPNNQIVDLAQAVAIAHRRRIPVVLDNTFATPLNQRPLK
                     FGVDVVVYSATKYLAGHSDLIAGFIVGTKKFCTRASLYRKILGGSHDPFGAWLLLRGL
                     KTLALRVARQNRNGQAVAEFLLQHPKIRRVFYPGLPSDPGHRLAKRQMTGFTGMVCFE
                     VSDGLKNVEKVMDRFRVILHATSLGGVESVAHIPVLTSHIQFSPAELKKADVTPGMIR
                     LSCGIEDPGDLKADLDHALRVL"
     gene            975..1931
                     /locus_tag="AC16GM_05735"
     CDS             975..1931
                     /locus_tag="AC16GM_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR2923213.1"
                     /translation="MPKIDRLVEKHFEKFLVLGLALGVVLLDLFVIQKTVFLNFYYLP
                     VLVSGYVLGRRSAILTAFLCIGLVAFAASVNPSFVTSSGSSTTLALDLVIWGGFLILA
                     AYVVGTLYQHKEQKIEELKNAYIGVIEILTKYLEANDRYTRGHSVRVADYATDIASLL
                     RLPKEEVQQIRAAALLHNIGRMEISSNLIRKAAKLTIEKHEIMGTETEKGTELLRAVG
                     DVLEAALPITLAFHQYYIQGSDGDKPLADIPIGAQILHVADRFDEMTVDSAYRKSISP
                     EEAIGHLEESAGARYQPEIVAALKRVYAAKGEGQPQMFAYQD"
     gene            complement(2073..2765)
                     /locus_tag="AC16GM_05740"
     CDS             complement(2073..2765)
                     /locus_tag="AC16GM_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923214.1"
                     /translation="MAINELLRYGFGGALFIVAFTFVGSPEVSNIKKEASGVLVIFLA
                     FALGAILHAASRLLIEFIFWPIRYLIVWGIAFVLENITGRGLWAEQNSSFHLLRRQFQ
                     VRYFDVLNAFRVVRDEFFEEKRQKWFRIEHSEIHLVYVATLVSGLTTIYVALNPFDSS
                     GNFAPQNAPSVTVLGWLTAVLLFVAVTSDILLAQRECAYLRTLIELNEGVKTQIRKLL
                     GAAGYLKDDLGA"
     gene            complement(2817..3248)
                     /gene="aroQ"
                     /locus_tag="AC16GM_05745"
     CDS             complement(2817..3248)
                     /gene="aroQ"
                     /locus_tag="AC16GM_05745"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR2923215.1"
                     /translation="MKKVLVVNGPNLNLLGSREMEIYGRQSLEEINGLIQAEADKLGA
                     EVKFVQSNSEGGLIDLIQEARAWTEGIIINPGAYTHYSYAIRDCLTAVRKPTIEVHLS
                     NLHAREEFRHHSVIAPACKGQIVGLGAKGYLLALRFLVEMG"
     gene            complement(3245..4366)
                     /gene="aroB"
                     /locus_tag="AC16GM_05750"
     CDS             complement(3245..4366)
                     /gene="aroB"
                     /locus_tag="AC16GM_05750"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR2923216.1"
                     /translation="MKTIRVDLGARSYSIHLKARGLADLARLVQREFPESKIAVITDR
                     NVARRYSRSLMQVFSGRKKRTVFIKLPSGEKTKTLTTCQKIYTQLLRRRFDRGDLVIA
                     FGGGVVGDIAGFVAATYARGLPFVQVPTTLVAQCDAAIGGKVGVNHPLGKNMIGAFHQ
                     PRFVFVDPAFLNTLPGRELRQGLAEVVKYALIRDRKLFRQLETKLPRWAVGRLDGFEP
                     VLVRCCQIKAEIVARDERDYGLRHLLNFGHSIGHALEAAADYGRFLHGEAVVLGILAA
                     NWIAVGKKTLSPDESNRAGKLLALLARRMPRVRFPIEKALSAVAVDKKHRQGTLRFVL
                     LRRIGQAVIREDVTTAETKKALVFLRDWLQTTGRAKGAG"
     gene            complement(4372..4905)
                     /locus_tag="AC16GM_05755"
     CDS             complement(4372..4905)
                     /locus_tag="AC16GM_05755"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR2923217.1"
                     /translation="MNIYVTGFMGTGKTVVGRRLARKLNRHFVDLDSEIVRTTRRSIP
                     HIFSEDGERAFRRLEDKVFRRFAVRKKSVVALGGGTLLNRQNMLLARKTGVIVCLEAS
                     LEVILQRVGNAGERPLLNLPGNLPEREQRRMALARIRAMLARRRSVYQQSDFFVNTSR
                     RSVAQVAAAVQSVLEKC"
     gene            complement(4934..5386)
                     /locus_tag="AC16GM_05760"
     CDS             complement(4934..5386)
                     /locus_tag="AC16GM_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR2923218.1"
                     /translation="MFETSGFLSALFWGALKLLGIVFVIVVPLMVLLEIVQRRRLFDR
                     LIAVVAPVMRFWGFEREAIFPLLAGLSFGVAYGAGVLLQESRENRLSGRQAFLVCTFL
                     GMSHAVFEDTLLFVAVGANGWAVVGVRLLTALVVTFVVAHYVVKVQRA"
     gene            complement(5379..5867)
                     /locus_tag="AC16GM_05765"
     CDS             complement(5379..5867)
                     /locus_tag="AC16GM_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR2923219.1"
                     /translation="MSAAGASAIPLEKSETWPALLTDGLKRGLSTFWFLCKIMVPVYA
                     VVTFLKFTPVLKAIASFCAPAMRFFALPGEAALALVMGNAVNLYAAIAVMITIHLEPA
                     QITLLAVMLGISHSQVMETTIMNKMKINGWAVMALRMAMSLLVGFILGRIALWVGVTL
                     RV"
     gene            complement(5864..6769)
                     /locus_tag="AC16GM_05770"
     CDS             complement(5864..6769)
                     /locus_tag="AC16GM_05770"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR2923220.1"
                     /translation="MPSANRQNLNLVGIIGYPLDHSLSPLLHNTAFRSLRLPWHYQKF
                     EVKPKELANCLAGVRAMGMRGLSVTIPHKEKVIRFLDRVTGAAAKIGAVNTIVNENGK
                     LVGYNTDYLGFLSPLVRLLKPAKPLSVALLGAGGAARAVLFALFSHFRVWETLIFNRN
                     QKRAAALARWAGRVARQEMQIHILRLLDASEEMRLADMDVIVNATPLGLYPKIKQMPV
                     TSADVFRRGQIVYDLNYNPAETRLLREARRQKARTINGLEMLLGQGREAFRLWTGREM
                     PVHQVRTSLTTHLNHHPGPHLADEE"
     gene            complement(6774..8135)
                     /gene="aroA"
                     /locus_tag="AC16GM_05775"
     CDS             complement(6774..8135)
                     /gene="aroA"
                     /locus_tag="AC16GM_05775"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002120199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR2923221.1"
                     /translation="MSPVQLGTQNEVLIRPAKSLSGVITVPGDKSISHRAALLLALAD
                     GTGTIENFSPAADCRSTLACLKKLGVEIKETKENITIAGRGFGGLRRPEAALDCGNSG
                     TSLRLLAGILSFCDFSARLDGDASLRTRPMQRVVEPLSRMGARFDAPTGTAPLTVHGG
                     ALSGIEYRLPVASAQVKSAVLLAGLHAKGETTVIETAPTRDHTERMLEFLGVTLTAVP
                     ERDESTAEVDFRKKPKTTGQRITVKPIEKIAPKTLFIPGDVSSAAYFIAAALLVRRSE
                     VIIQDVGLNPTRTAFLRLLKQMGANLAIEKTGERNGEPFGTVTVKSSTLSPRKISGEA
                     VAGLIDELPLLALLATQSEGTTVIRDAQELKVKESDRIATVAENLRRMGAKVGILDDG
                     WVIEGKSELDGAEIDPQGDHRIAMTFAVAGLVAHGQALISEAGSVAVSYPNFFETLEA
                     LRR"
     gene            complement(8132..9145)
                     /gene="aroF"
                     /locus_tag="AC16GM_05780"
     CDS             complement(8132..9145)
                     /gene="aroF"
                     /locus_tag="AC16GM_05780"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR2923222.1"
                     /translation="MLVVMEVAATSEQIDAVCQVITEMGLKAHPMPGAQRTAIGITGN
                     IGVVDSSRIETLPGVINVIHVSQPFKLASREVKPEDTVVSVNGVKVGGQKLAIMAGPC
                     AVESREQALTIAKAVKKAGATIYRGGAFKPRTSPYSFQGLGGKGLEILAEVRKETGLQ
                     IVTEALDPEGVDLVEEHADIIQIGARNMQNFSLLKRVGKARKPVLLKRGMAATLEELL
                     MAAEYIMVEGNRDIILCERGVRTFADHTRNTLDLSAVPYVKRMSHLPIIVDPSHGTGR
                     REKVLPMSRAAVAVGCDGLIVEVHHDPGHALSDGPESIYPSQFEQLMQEISQIAAVIG
                     RSV"
     gene            complement(9168..>9842)
                     /gene="argS"
                     /locus_tag="AC16GM_05785"
     CDS             complement(9168..>9842)
                     /gene="argS"
                     /locus_tag="AC16GM_05785"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR2923223.1"
                     /translation="LFKVLELLGADWADRCVHVEFGWVKFEDKIMATRRGNLIFLEDV
                     LDKAIALAEKIIREKNPELENIDSVKRMVGVGAVIFADLAVRRQKDVNFEWEKVLTFE
                     GETGPYLQYTHARLASLERKFSRTITAAINFELLTQKELWLLVRTLADFPQKVQIAAA
                     QYEPAIVAGYLLELAGRFNTYYQNYRIITEDPESTQAKMLVVAAARSVLQEGLRLLGL
                     AAPERM"
BASE COUNT         2104 a         3031 c         2611 g         2096 t
ORIGIN      
        1 accgagaccc cgaccaaccc gaacaaccag atcgtcgatc tcgcgcaggc cgtggccatc
       61 gcccaccgcc gccggatacc ggtggtgctg gacaacacct tcgccacgcc gctgaaccag
      121 aggccgctga aattcggcgt ggacgttgtc gtctacagcg ccacgaaata cctggccggc
      181 cactccgacc tgattgccgg ttttatcgtc ggcaccaaaa agttctgtac ccgggcgtcg
      241 ctctatcgca aaatcttagg cggttctcat gatcctttcg gcgcctggct tttgctgcgc
      301 ggcctgaaaa cgctggcgct gcgggtggcc cgacaaaatc ggaacggtca ggcggtggcc
      361 gaattcctct tgcagcaccc caagatcagg cgcgttttct acccgggcct gccttccgac
      421 cccgggcatc ggttagccaa aaggcagatg accggcttca ccgggatggt ctgcttcgaa
      481 gtatccgacg ggctcaaaaa cgtcgaaaaa gtgatggatc gtttccgggt gattctccac
      541 gccaccagcc tgggcggcgt ggaaagcgtc gcgcacattc cggttttgac ctcgcacatc
      601 cagttctcac cggcggaact gaagaaagcc gacgtgacgc ccgggatgat ccgcctctcc
      661 tgcggcattg aggatccggg cgacctcaag gccgacttag accacgccct gcgcgttctg
      721 taacgctttg ctggaactgt agcgacgctc tctttagcct gagcttgccg aaggcttggg
      781 gttcggttgt cggccctgct cggagaccgg ggctgccctc ggcaagccgg caacggaaca
      841 atcccatccc cgaccccgga aaagcgcccc tggcttcaat acccagccgg cggaccggac
      901 aaaggcaccc gctaatcaaa tagaacttta ctccgatata agaaatgagc ggaaaatctt
      961 aggggatgct gcctatgccg aaaatcgata gactcgttga aaagcacttc gagaagtttc
     1021 tggttctggg gctggccctg ggagtggtgc tcctcgacct gttcgtcatc cagaaaacgg
     1081 tcttcttgaa tttctactac ctccccgttc tggtttcggg ctacgtcctg ggccgccgga
     1141 gcgcgatttt gaccgctttc ctctgcatcg gcctggtggc cttcgctgcc tcggtcaatc
     1201 cctcctttgt cacttccagc ggctcctcga ccaccctggc gctcgacctg gtgatctggg
     1261 gcggattttt gatcctggcc gcctacgtcg tcgggacgct ctaccagcac aaggagcaga
     1321 aaatcgagga gttgaagaat gcctacatcg gggtgattga gattttgacc aagtacctcg
     1381 aagccaacga ccgctacact cggggtcact cggtgcgggt ggccgactac gccaccgaca
     1441 tcgcctcgct cttacgcctg ccgaaagagg aagtccagca aatccgggcg gcggcgcttt
     1501 tgcacaacat cggccggatg gaaatcagtt ccaacttgat tcggaaagcc gccaaactca
     1561 ccatcgagaa gcacgaaatc atggggaccg agacggagaa aggcaccgaa ctcttgcgcg
     1621 ccgtcggcga cgttttggaa gcggcactcc ccattaccct ggccttccac cagtactaca
     1681 ttcaagggag cgacggggat aaacctcttg cggacatccc catcggcgcc caaattttgc
     1741 acgtggccga tcgcttcgac gaaatgaccg tcgattcggc ctaccgaaaa tcgatctcac
     1801 cggaagaagc cataggccac ctggaggagt ctgccggcgc ccgttaccag ccggaaattg
     1861 tggccgccct caagcgggtc tacgccgcca aaggcgaagg ccagccccag atgttcgcgt
     1921 atcaggatta gcgcccctca aaccagccag ccagaatcaa tatgaaagtg tggctccggt
     1981 tttctgaccg gggtgaattt tcgacgctca ggtgagtggg tattttcctc ccatattgta
     2041 ggacctgccc ttccccaagc cggatagact atttacgctc ccagatcatc ttttaggtag
     2101 ccggctgccc ctaagagctt tctgatttgt gtttttacgc cttcatttaa ttcaatcaac
     2161 gttcgtaagt aggcacattc gcgttgagcc aaaagaatgt ccgaggtaac cgctacaaaa
     2221 agcaacaccg ctgtaagcca tcctaacact gtgactgacg gagcattctg cggagcgaag
     2281 tttccagagc tatcgaatgg atttagtgct acatatatcg ttgtaagccc agaaactagt
     2341 gttgctacat atacaagatg aatttcgcta tgctctattc ggaaccactt ctgacgtttc
     2401 tcctcgaaaa actcgtcgcg aactacccga aaggcgttga ggacatcaaa ataccttact
     2461 tggaattgtc gtcttaataa atgaaaagaa ctattttgct cagcccaaag acctcttcct
     2521 gttatatttt ccaaaacgaa tgcgattccc catactatca aatatcgaat aggccaaaaa
     2581 atgaattcaa ttaaaagacg tgaggctgca tgcagaatag ccccgagtgc aaaagcaagg
     2641 aatatgacca ggacaccact ggcttccttt ttgatattgc ttacctctgg ggaaccgaca
     2701 aaggtaaatg cgacaataaa caacgctcca ccaaagccat aacgaagcaa ttcatttatt
     2761 gccattccaa atttttcgaa cggctttggt tattgtagat cactcaaggt aaaatcctac
     2821 cccatctcca ccagaaaccg cagcgccaaa aggtacccct tggctcccag cccgacgatc
     2881 tgccctttgc aggccggcgc aatcaccgag tggtggcgga attcttcccg cgcgtggagg
     2941 ttggagaggt ggacctcgat ggtcggcttg cgcaccgccg taaggcaatc ccggatggcg
     3001 tatgagtagt gcgtgtacgc gccggggttg atgataatcc cctcggtcca cgcgcgcgcc
     3061 tcctgaatga gatcgatcaa gcccccttcg ctgttggact ggacaaactt cacctccgca
     3121 cccagtttgt cagcttcggc ctggatgagc ccgttgattt cttctaaact ctggcggccg
     3181 tagatctcca tctcccggct ccccaggaga ttcagattcg gcccgttgac caccaagact
     3241 ttcttcaccc ggctcccttc gctcggcctg tagtctgcaa ccaatctctc aaaaacacaa
     3301 gtgctttttt ggtctcggca gtggtaacgt cctcacgaat gaccgcctgg ccaatgcgtc
     3361 gcaagagcac aaagcggagc gtcccctgcc ggtgtttttt atccaccgca acggcggaaa
     3421 gggctttttc gattggaaac cgaacccggg gcattctccg tgccaaaagc gccaaaagct
     3481 ttccggcacg atttgattca tccggagaga gcgtcttttt ccccaccgcg atccaattgg
     3541 ccgccaggat tcccaggacc accgcttccc catgcaggaa tctgccgtag tcggcagccg
     3601 cctcaagcgc gtggccgatg ctatggccga agttcaacag gtgccgcaga ccgtaatcgc
     3661 gctcgtcccg ggcgacgatc tccgctttga tctgacagca gcgcacgagc accggctcaa
     3721 agccgtccag tcgtcccacc gcccaccgtg gtaatttggt ttccagttgc cggaagagtt
     3781 ttctatctcg aattaaagca tacttgacta cctcggccag gccctgtcgc aattcgcgcc
     3841 cgggcagtgt atttaggaaa gcgggatcga cgaaaacaaa tcgcggctgg tggaacgccc
     3901 cgatcatatt tttccccagc gggtggttca cgcccacctt gccgcctatc gccgcatcgc
     3961 actgggccac taaggtcgtc ggcacctgaa cgaacggcag gccacgcgcg taggtcgccg
     4021 ccacaaatcc ggcaatatcc cccaccaccc ccccgccgaa ggcgatgacc aggtcacccc
     4081 ggtcaaaacg tctccgcaga agttgcgtgt agattttctg acaggttgtg agcgtcttcg
     4141 ttttttctcc ggatgggagt tttatgaata ccgtccgctt ttttcttccc gagaacacct
     4201 gcatcagcga tcgggaatat cggcgtgcta catttcggtc ggttataacg gcgattttgc
     4261 tttcgggaaa ttcccgctgc accaatcgcg ccaggtcggc caggccgcgg gctttgaggt
     4321 ggattgaata cgaacgtgcc cccagatcta cccggatcgt tttcattttt ttcaacattt
     4381 ttccagtacg ctttgtaccg ctgctgcaac ctgtgccacc gaccgccgtg atgtattcac
     4441 gaaaaaatct gactgttggt agacggagcg acgccgagcc agcattgctc ggatacgcgc
     4501 cagagccatt ctccgctgtt cccgctccgg gagattcccc ggcaagttca aaaggggacg
     4561 ctcaccagca ttcccgactc gctgtaggat cacctctagg ctcgcctcaa ggcaaactat
     4621 gacgccggtc tttctggcca aaagcatatt ctggcggttg agcagggtcc cgccccccag
     4681 tgccacgacg gatttttttc taacggcgaa cctccggaaa actttatcct ccaagcggcg
     4741 aaaagcccgt tctccatcct cgctgaaaat gtgcggaatt gaccggcgcg tcgtgcgcac
     4801 tatttccgaa tcaagatcga caaaatgtcg gttgagtttt ctcgccaacc gcctccccac
     4861 caccgtcttc cctgtcccca tgaagcccgt tacgtagatg ttcacaaaac ttcgagaggg
     4921 gggaatgttc tccctacgca cgctggacct tgacgacata gtgcgccacg acgaaggtca
     4981 caaccaaagc cgttaaaaga cgcacgccca cgacggccca gccgttggcc cccaccgcca
     5041 cgaagagcag cgtatcctcg aacacggcgt ggctcatccc cagaaaggtg cagactaaaa
     5101 aggcctgccg cccgctcaag cggttctcgc gcgattcctg caggagcacg ccggcaccgt
     5161 aggcgacgcc gaacgacaac cccgctaaga gcgggaaaat cgcctctcgc tcgaagcccc
     5221 agaagcgcat aaccggcgcg acgacggcaa tcaagcggtc gaagagccgt cggcgctgga
     5281 ctatttcgag caagaccatc agcggcacca cgatgacgaa cacgatgccc agaagtttca
     5341 gtgctcccca gaaaagcgcc gagagaaaac cggaggtttc aaacacgcag tgtcactccg
     5401 acccagaggg caatccgccc cagtatgaat ccgaccagaa gcgacatcgc catgcgcagc
     5461 gccatcaccg cccagccgtt tattttcatt ttgttcataa tcgtcgtttc catcacctgc
     5521 gagtgcgaaa tccccagcat caccgccaga agggtgattt gggccggctc gaggtgaatg
     5581 gtgatcatca ccgcaatcgc cgcgtagagg ttgaccgcat tccccatcac cagagccagg
     5641 gccgcttctc cgggcaaagc aaagaagcgc atggcggggg cgcagaagga agcgatggcc
     5701 ttcaggaccg gcgtgaattt caaaaaggtg acgaccgcgt aaaccggaac cataattttg
     5761 cagagaaacc aaaaagttga aaggccgcgt ttgaggccgt cggtcagaag cgccggccag
     5821 gtttcgcttt tctccagagg tatggcggaa gcgccggctg cactcattct tcgtccgcca
     5881 ggtgcggtcc ggggtggtgg ttcaggtgcg ttgtgagaga ggttctaacc tggtgcaccg
     5941 gcatttctcg gcccgtccag aggcgaaagg cctcccgccc ctggcccaga agcatttcga
     6001 gtccattgat ggtcctggct ttttgccggc gggcttcccg gaggagccgc gtctctgccg
     6061 gattgtaatt caggtcgtag acaatttgcc cccggcggaa aacgtctgcc gaagtgaccg
     6121 gcatctgttt gattttcggg tataaaccca gtggcgtggc gttgacgatt acgtccatat
     6181 cggccagacg catttcttcg gatgcgtcca gaagccgcag aatatgaatc tgcatttcct
     6241 gacgtgcaac tcgcccggcc caacgcgcga gcgccgcggc acgtttctga tttcggttga
     6301 aaatcaaagt ttcccacacc cgaaaatggg aaaaaagggc aaagagaacc gcgcgcgccg
     6361 ctccgcctgc accgaggagg gcgaccgaga gaggtttggc gggtttcagc aaacggacga
     6421 gcggtgagag gaatccaaga tagtcagtat tatagccgac cagtttgcca ttctcgttca
     6481 caatcgtgtt aaccgcaccg attttggccg ccgcgccggt gacgcggtct aagaaccgga
     6541 ttaccttttc cttatgcgga atggtgacgc tcaaaccccg catccccatg gcccgtactc
     6601 cggccaaaca gttcgccagt tccttcggct tcacctcgaa tttctgatag tgccagggca
     6661 gccgcaatga ccggaaggca gtgttgtgca gaagcggcga aagcgaatgg tcgagggggt
     6721 agccgatgat gccgaccaaa ttcaggttct gtctgtttgc gctcggcatt ggttcagcga
     6781 cgcagggctt ccagcgtttc aaaaaaattc gggtaggaaa ccgcaaccga cccggcctcg
     6841 gaaatcaacg cctgcccgtg ggccacgagc ccggccaccg caaaggtcat ggcgatgcgg
     6901 tggtctccct gcggatcaat ttccgcgccg tctaattcgc ttttgccttc aatcacccag
     6961 ccgtcgtcca gaatgcccac tttcgccccc atccggcgca gattctcggc cacggttgcg
     7021 atccggtccg actcctttac cttcaactcc tgcgcgtcgc ggatcaccgt ggttccttcg
     7081 ctctgggtcg ccaaaagtgc caaaagcgga agttcgtcga ttaatccggc aacggcctcg
     7141 ccggaaattt tcctggggga caaggtactt gattttacgg taaccgtacc gaacggctca
     7201 ccgttccgct ccccggtttt ctcgatggcg aggttggccc ccatctgttt gagcagccgg
     7261 agaaaggccg ttcgagtcgg attgagaccc acgtcttgga taatgacttc cgagcgccgc
     7321 accagaagtg cggccgcgat gaagtacgca gcggaagaaa catccccggg aatgaaaagc
     7381 gttttcggtg cgattttttc tatcggtttg acggtgatcc tctgccccgt agtcttgggc
     7441 tttttccgaa agtcaacctc agctgttgac tcgtcccgct cgggcacagc cgtcagcgta
     7501 acccccaaaa actccagcat tctttcggta tggtcgcggg tcggcgccgt ttcgatgaca
     7561 gtcgtctcgc ctttcgcgtg caggccggcc aggagaaccg ccgatttcac ctgcgccgaa
     7621 gccactggca ggcggtactc aatcccgctc agggcgccgc cgtgcacggt caaaggcgcg
     7681 gtgccggtgg gagcgtcaaa ccgcgcgccc atacgggaga gcggttccac cacccgttgc
     7741 attggccggg tgcgcaagga ggcgtcacca tccagcctgg ctgaaaaatc gcagaaactt
     7801 aatatcccgg ccaaaagccg gagcgacgtt ccggagttgc cgcagtccag cgccgcttcc
     7861 ggcctgcgga ggccgccgaa tccccggccg gcaatcgtta tgttctcttt tgtctctttt
     7921 atttcgacac ccaatttttt caaacaggcc agggtgctcc ggcagtcggc ggccggagaa
     7981 aaattctcta tggtcccggt tccatcagcc aatgccagaa ggagcgccgc ccggtgggaa
     8041 atcgatttgt cgcccggaac tgtaatgacg cccgagagac ttttcgccgg tcggatcaag
     8101 acttcgttct gtgtgccgag ctgaaccgga ctcataccga tctcccgatc accgcggcaa
     8161 tctggctgat ttcctgcatc aactgctcga attgggaggg ataaatcgac tctggcccgt
     8221 cggagagggc gtgtcccgga tcgtggtgga cttcgacaat cagcccgtcg caccccaccg
     8281 ccactgccgc ccgcgacatc ggcagcactt tctcgcggcg cccagttccg tgggagggat
     8341 cgacgataat cggcagatgg ctcatccgct tgacgtaggg cactgccgaa agatcgagcg
     8401 tgttgcgggt gtggtcggca aaggtgcgca ctcctcgctc gcagaggatg atgtcgcggt
     8461 tcccctccac catgatgtac tctgccgcca tcaggagctc ttctaaggtt gccgccatgc
     8521 cgcgcttgag cagcaccggc ttccgcgcct tgccgacccg cttcagaagt gaaaaattct
     8581 gcatgttgcg cgcgccgatc tggatgatgt cggcgtgctc ctccaccaga tcgacccctt
     8641 cgggatccag cgcttcggtg acgatctgaa gtcccgtctc cttccgcacc tcggccaaga
     8701 tctccagccc ttttccccct aatccttgaa aactgtacgg cgatgtccgc ggcttgaacg
     8761 ccccgccccg gtaaatcgtc gcaccggcct ttttcaccgc tttggcaata gtcaacgcct
     8821 gctcgcggct ctccaccgcg cacggtcccg ccatgatcgc cagtttctgc ccgcctactt
     8881 taaccccgtt cacggaaacg accgtatctt ccggcttcac ctcccggctc gctaatttga
     8941 acggttgcga aacgtggatg acgttgatga ccccgggcag cgtttcgatc cgggacgaat
     9001 cgacgacacc gatattgccg gtgatgccga tggcggtccg ctgcgccccc ggcatgggat
     9061 gggctttcag ccccatctcc gtgataacct ggcagacggc gtcgatctgc tctgacgttg
     9121 ccgctacttc cattacgact agcattgata tcctcgattc cgagtattca cattctctcc
     9181 ggcgcagcca ggcccaaaag ccgtaagcct tcctgcaaca cgctgcgggc cgccgcaacc
     9241 accaacatct ttgcctgggt gctctcagga tcctcggtga tgatgcgata gttctgatag
     9301 taggtattga accgtccggc cagttccagg agatagcccg ccacgatggc cggctcgtac
     9361 tgcgccgcgg cgatctggac tttttgcgga aagtcggcca gcgtgcgcac caaaagccag
     9421 agttccttct gggtgaggag ttcgaaattg atggcagcgg taatggtccg gctgaatttc
     9481 cgctccagac tcgccagccg cgcgtgagtg tattgaagat atggacctgt ttctccttca
     9541 aaagtcaaga ccttctccca ttcgaagttg acgtcctttt gccgacgcac cgccagatcg
     9601 gcgaaaatca ccgcccccac ccccaccatc cgcttcaccg aatcaatgtt ttctaattcc
     9661 ggattctttt cgcggatgat tttctccgcc aaagcaattg ctttatccag cacgtcctct
     9721 aagaagatga gattcccgcg ccgggtggcc ataattttat cctcaaactt cacccagccg
     9781 aattcgacgt gtacgcagcg gtcggcccag tcggcgccca gaagttccaa gaccttgaag
     9841 ag
//
LOCUS       DBNGZD010000220         2475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1767501, whole genome shotgun sequence.
ACCESSION   DBNGZD010000220 DBNGZD010000000
VERSION     DBNGZD010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1767501"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="AC16GM_05790"
     CDS             <1..256
                     /locus_tag="AC16GM_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2923224.1"
                     /translation="HYPRQLSGGQEQRVAIARAIVTDPTLLVADEPTGDLDKQSAEEI
                     MTLLARLNAEYKKTVVIVTHDPRAADKAHVQRHLDKGELS"
     gene            253..1410
                     /locus_tag="AC16GM_05795"
     CDS             253..1410
                     /locus_tag="AC16GM_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2923225.1"
                     /translation="MKIVKLAVKNAGRHKLRTALTILGLAVAVMAFGLLRTVISAWYA
                     GAEASAPDRLVTRNAVNIIFPLPLAYREQIQKVPGVEKVSYAVWFGGYYVDPKNFIAN
                     FAIDHPVYFDLFPEIIIDSASLRAFQSERSAAICGQILADRFRWKVGDKIRLVGTIYP
                     GNWDLTLRGIYHGRDKSTDESALFLRWDYLDENLRATAPVRAGNVEWYVMKIANPNDA
                     ASVSAAVDALFQNSLAETLTETEKEFNLSFIAMSGTIITALQFISYLIIGIILLVLTN
                     TMAMTARERISEYAVMKSLGFRPYHLAGVIAGESFFIAAAGGILGVVLIFPMAAGFGA
                     AMKAFFPVFQVKPQIAVLAGSFALGVGFLAAVFPILKATRLSIVKGLRTIG"
     gene            1407..>2475
                     /locus_tag="AC16GM_05800"
     CDS             1407..>2475
                     /locus_tag="AC16GM_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2923226.1"
                     /translation="MKIPFFYSYRNLFARRLTTGFTVLGLALVVFVFCAVLMLAYGLK
                     KTLVATGSDDNAIIIRKASQTEMQSYITRDQVAVIESQPEITRGADGKPLVAGELYVL
                     ISLNKRKGGDAANVVVRGVGPKSKLIHANVKLIAGRMFTEGLPEVMVGKAAAERFRGA
                     ELGGTVRFGKEDWRVVGVFAANRSGFESEVWGDVNQMLAAFNRPVVSSMTLKLADPHL
                     FPELKKRLEGDPRLSVDVKQEKEYYADQSRATGLFIQILGIVISVIFSLGAIIGAMIT
                     MYATVASRSAEIGTLRALGFRRRNILSAFLVEALLLSLAGGVVGLFAASLLSLVTVST
                     TNWDTFSELAFSFALSPSIFGL"
BASE COUNT          460 a          755 c          726 g          534 t
ORIGIN      
        1 gcactacccc cggcagctct ccggcggaca ggagcaacgg gtggccatcg cccgggccat
       61 tgtcaccgac ccgaccttgc tcgtggcgga cgagccgacc ggtgatctgg ataagcagtc
      121 ggcggaagaa attatgaccc tgctcgcccg cctgaatgcc gagtataaga aaaccgtcgt
      181 gatcgtcact cacgacccgc gcgcggcgga caaggcccac gtccaaaggc acctcgataa
      241 aggggaactg tcttgaaaat cgtcaaactg gccgtcaaaa atgccggccg gcacaaactg
      301 cgcacggctt tgacgatcct cggcctggcc gtggcggtca tggccttcgg gctcttgcgc
      361 accgtcatct cggcctggta cgccggggcg gaggcctccg ctccggatcg gctggtcacc
      421 cgcaatgcgg tgaacatcat cttcccctta cccctcgcct accgggagca aatccagaag
      481 gtgccggggg tggaaaaagt ttcctacgcc gtttggttcg gcggctatta cgttgacccc
      541 aagaatttca tcgccaattt cgccatagac catcccgtct acttcgacct tttccctgag
      601 attatcatcg attcggcctc cctgcgggcc ttccaatccg aacggagtgc cgccatctgc
      661 ggccagattc tggccgaccg ctttcgctgg aaggtggggg acaaaatccg gctggtgggg
      721 acgatctatc cgggaaactg ggacctgacc ctgcgcggga tttaccacgg tcgggacaag
      781 tcgaccgacg agtcggctct ctttttgcgc tgggattatt tggacgaaaa cctgcgcgcc
      841 acggcgcccg tacgggcggg gaatgtcgaa tggtacgtca tgaagattgc caaccccaac
      901 gatgccgcca gcgtctcggc ggcagtggat gcgctcttcc agaactccct ggcggagacc
      961 ttgaccgaga cggagaagga gttcaacctt tcttttatcg ccatgtccgg caccatcatc
     1021 acggctctcc agttcatctc ctacctcatc attggtatca ttctcttggt tttgacgaac
     1081 accatggcga tgacggcgcg cgaaaggatc tcagagtacg cggtcatgaa atccctgggg
     1141 ttccggccgt accacctggc cggggtgatt gccggggaat cctttttcat cgccgccgcg
     1201 ggaggaattc tgggcgtcgt cttgattttc ccgatggcgg ccggctttgg cgcggcgatg
     1261 aaagctttct tcccggtttt tcaggtcaaa ccccaaatcg ccgtcctggc cgggtctttc
     1321 gccctgggcg tcggtttcct ggcggcggtc tttccgattt tgaaggcgac gcggctctcc
     1381 atcgtcaagg gcttgaggac catcggatga aaataccctt tttctacagc taccggaatc
     1441 tcttcgcccg gcggctcacc accggtttca ccgttctggg tctcgctctg gttgtcttcg
     1501 tcttctgcgc ggtcttgatg ctggcctacg gactgaaaaa gactctggtg gccaccggct
     1561 ccgacgacaa cgccatcatc atccggaagg cctcccagac cgaaatgcag agctacatca
     1621 cccgcgatca ggtggcggtc attgaatccc aaccggaaat cacccgggga gcggatggca
     1681 aacccctggt ggccggagag ctgtacgtgc tgatcagttt gaacaagcgg aagggcggcg
     1741 acgcggccaa tgtcgttgtg cggggtgtgg gtccaaaatc gaaactgatc cacgcaaacg
     1801 tcaaactcat cgccggaagg atgtttactg aaggactgcc ggaagtgatg gtcgggaagg
     1861 ccgccgccga aaggttccgg ggcgccgaac tgggaggcac ggtccggttc ggcaaagagg
     1921 attggcgggt ggtcggtgtt ttcgcggcca accgctccgg ctttgaatcg gaagtctggg
     1981 gcgacgtcaa ccagatgctc gccgccttca accggccggt ggtttcttcc atgaccttga
     2041 aactggccga cccgcacctg tttccggaac tgaagaaacg gctggaagga gacccccgtc
     2101 tgtcggtcga cgtcaaacag gaaaaagagt actacgccga tcagtcgcgt gccaccgggc
     2161 ttttcatcca gattttaggg atcgtcatca gcgtcatctt ttccctgggc gcgatcatcg
     2221 gcgcgatgat cacgatgtac gccacggtgg ccagccgttc cgccgaaatc ggcacgctgc
     2281 gggcgctcgg tttccgccgc cgcaacattc tctccgcctt cctggtcgaa gcgcttctgc
     2341 tctctctggc tggcggggtg gtcgggttat ttgccgcgtc ccttttgagt ttggtgaccg
     2401 tttccactac caactgggac accttttccg aactggcttt cagctttgcc ctctcgccgt
     2461 caattttcgg gctcg
//
LOCUS       DBNGZD010000221         2987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_658794, whole genome shotgun sequence.
ACCESSION   DBNGZD010000221 DBNGZD010000000
VERSION     DBNGZD010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_658794"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="AC16GM_05805"
     CDS             complement(<1..650)
                     /locus_tag="AC16GM_05805"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR2923227.1"
                     /translation="MTDRVILIVLDACGAGELPDARAYGDTGSNTLGNVARAVGGLHL
                     PHLERLGLGKIIPIEGVSPAVVAEGCYGKLAERSPGKDSTTGHWEIAGIFLEKPFPVY
                     PHGFPPEVIGPFEQAVGRKVLGNKPASGTEILKELGAEHHRTGRPIVYTSADSVFQIA
                     AHEEVIPLGELYQMCQTAREILAGDHAVGRVIARPFVGATPENFKRTANRRDFSLPP"
     gene            complement(785..1279)
                     /gene="rplQ"
                     /locus_tag="AC16GM_05810"
     CDS             complement(785..1279)
                     /gene="rplQ"
                     /locus_tag="AC16GM_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR2923228.1"
                     /translation="MRHRRYVFKLGRTKSHREAMLANLATALLEHRRIRTTAVRARAL
                     VPFVERLITLAKAGDLAARRQAAVVVRSPLVLQKLFSEIAPELASRNGGYTKVLKLGI
                     RQGDGASLALVELLVTRPKPKVAEKKKGAKKSEKETEAKAAPESKLEKGRKSKEKYKV
                     PAKF"
     gene            complement(1276..2268)
                     /locus_tag="AC16GM_05815"
     CDS             complement(1276..2268)
                     /locus_tag="AC16GM_05815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR2923229.1"
                     /translation="MPMKWKNMQIPKEVTVDPETATAQYSRLVIEPLERGFGTTLGNA
                     LRRVLLSSLPGAAVTALRIDGVVHEFTTVPGVYEDVPEIVLNVKRVRFRANADEPRTL
                     LLEANRKGKLTAAQIQSDGTYEVLNKDQFICQLTGDAKFRCEIDVGVGRSYVAAEQNK
                     RPSAPVGTIFLDSFFSPVTKVNFEIENTRVGQRTDYERLILDVWTDSSVTPDAAVNLA
                     AKLLREHLALFVKAEEELEIQPAEPEDEDVLRVRNLLKMRVDELELSVRSANCLRAEG
                     IETLEQLVHRTEGQMLKCRNFGKKSLTELNQILGELGLKFGMDLSRYYQKELVA"
     gene            complement(2288..2917)
                     /gene="rpsD"
                     /locus_tag="AC16GM_05820"
     CDS             complement(2288..2917)
                     /gene="rpsD"
                     /locus_tag="AC16GM_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR2923230.1"
                     /translation="MARYTGPSCRLCRREGEKLFLKGQRCSSDKCAFDRRGYAPGQHG
                     ADSRRKRSDYAMRLREKQKVRRIYGLLEAQFRISFAKAERGPGITGENLLALSERRLD
                     NVVYRLGFAPSRKAARQLVRHRHFTVNDRTVDIPSYLLAPHDVVRVREESRALDVIHA
                     ALKDQGREQTVPYLRLDKARLEGELLEVPKRADIPTLVSEQLIVELYSK"
BASE COUNT          517 a          946 c          892 g          632 t
ORIGIN      
        1 ggcgggagag aaaagtcgcg ccggttggcg gtacgcttga agttttccgg tgtagccccc
       61 acaaagggcc gggcaatcac ccgaccgacc gcgtggtcgc ccgccagaat ctcccgcgcg
      121 gtctggcaca tctgatacag ctctcccaaa ggaatgacct cttcgtgcgc ggcgatctga
      181 aagacgctgt ccgccgaagt gtagacgatg ggtctccccg tgcgatggtg ttctgcgccc
      241 aactctttta agatttccgt gccggaggcc ggtttgttcc ccaaaacctt tctgccaacc
      301 gcctgctcga aaggtccgat aacctctgga ggaaacccgt gcgggtagac gggaaagggt
      361 ttctccaaaa aaatcccagc gatttcccaa tggccggtgg tgctgtcttt tcccggcgag
      421 cgctccgcca gcttcccgta gcaaccctcc gcgacgaccg ccggactgac cccctcgatg
      481 gggataattt tccccaggcc gaggcgttcg agatggggga ggtgcaggcc gcccaccgcg
      541 cgggcgacgt tcccgagggt gttcgaaccg gtatcgccgt aagccctggc atcgggcagt
      601 tcgccggcgc cgcaggcatc cagtacgatg agtatgaccc ggtcagtcat ggttcggctt
      661 cgctcaccct gcaccgccga gcggaacttt cacagccggt ctccgctgat aaaaaaagcg
      721 gtcattccgt tacgaatgaa ataaaaaagc aggccgggtt gcctgcttgc ggttcaccca
      781 ttggttagaa cttggctggt accttgtatt tttcctttga cttcctgcct ttttccaact
      841 tactctctgg cgccgctttg gcttcggttt ctttttcgct cttcttggcg cctttctttt
      901 tctctgcgac tttcggcttg gggcgggtga ccaaaagctc caccaaagcc agggacgccc
      961 cgtcgccctg ccggattccc agcttcaaca ctttggtgta cccgccgttt cggctggcca
     1021 gttcgggggc gatttcgctg aagagtttct ggagcaccag gggactgcgc acgaccaccg
     1081 ccgcctgacg ccgcgccgcc agatctccgg ctttggccag agtgatcagc cgctccacga
     1141 aaggcaccag ggctcgcgct cgcacggcgg tggtccgaat cctccggtgt tccagaagtg
     1201 ccgtagccag attggcgagc atggcctcgc ggtggctttt ggtccggccc agcttgaaaa
     1261 cgtagcgccg gtgcctcacg cgactaactc tttctggtag taccgggaga gatccatccc
     1321 gaacttgagg ccgagttcac ccaagatctg attcagttca gtgagcgatt tcttcccgaa
     1381 attacggcac ttgagcatct gtccttcggt gcggtgcacc agctgctcca aggtctcaat
     1441 gccttcggcc cgcaggcagt tggcggagcg gacggagagc tccagttcgt ccacccgcat
     1501 tttcaggagg ttccggacgc ggagcacgtc ctcgtcctcc ggttccgccg gttgaatttc
     1561 taactcctcc tccgctttga cgaagagtgc caggtgctcc cgcaggagtt tggcggctaa
     1621 gttcaccgcc gcgtccgggg tgaccgaact gtcggtccag acatccagga tgagccgttc
     1681 gtagtcggtg cgctgaccca cccgtgtatt ctcgatttcg aaattaactt tggttaccgg
     1741 cgagaagaac gaatccagaa agatcgtccc caccggcgcc gagggcctct tgttctgctc
     1801 ggcagccacg tagctgcggc caacgcccac gtcgatctcg cagcgaaact tggcgtcgcc
     1861 ggtcagctgg caaatgaact ggtccttgtt gagaacctcg taggtgccgt cgctctgaat
     1921 ctgcgcggcg gtgagttttc ccttgcggtt cgcttccaaa aggagcgttc tcggctcgtc
     1981 ggcgttggcc cggaagcgca cccgcttgac gttgagcacg atttccggca cgtcttcgta
     2041 gacgccgggc accgtggtga actcatgcac taccccgtca atccgcagtg cggtgacggc
     2101 cgcaccgggc aaagaagaca gaagcacccg ccggagtgca ttccccagcg tggtcccgaa
     2161 cccccgctcc aggggctcga tcaccagacg gctgtactgg gcggttgcgg tttcgggatc
     2221 gaccgtcacc tctttgggta tttgcatgtt cttccacttc atgggcatca ctcctctgcc
     2281 gaaagcatta tttcgaatac aattcgacga tcaactgctc ggagactaac gtgggaatat
     2341 cggcccgctt ggggacctcc aggagctctc cctccaggcg ggctttgtcc agacgcaggt
     2401 agggcacggt ctgctcccgg ccctggtctt tgagggccgc gtggatgacg tccagggcac
     2461 ggctttcttc ccggacccgc acgacgtcgt gtggcgcaag caggtacgac gggatgtcga
     2521 ccgtacggtc gttcacggtg aaatgccggt ggcgcaccag ttgccgggcc gccttgcgcg
     2581 agggcgcgaa ccccagccgg tagaccacgt tgtccagccg ccgctccgaa agcgccagga
     2641 gattttcacc cgtgatgccc ggaccccgct ccgctttggc gaaagagatg cggaactggg
     2701 cctccaggag cccgtagatg cggcgcacct tctgtttttc cctgaggcgc atggcatagt
     2761 cggacctctt ccggcgcgaa tcggcaccgt gctggcccgg agcgtacccc cggcggtcga
     2821 acgcacactt atcggaagag cagcgctggc ctttcagaaa gagtttttca ccttcccggc
     2881 ggcagaggcg gcatgagggt ccggtgtatc tggccatcgg ctcctccgct acacccggcg
     2941 ccgcttgggc gggcggcagc cgttgtgggg aatcggcgtc acgtcgc
//
LOCUS       DBNGZD010000222         1909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_173926, whole genome shotgun sequence.
ACCESSION   DBNGZD010000222 DBNGZD010000000
VERSION     DBNGZD010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_173926"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="AC16GM_05825"
     CDS             complement(<1..589)
                     /locus_tag="AC16GM_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2923231.1"
                     /translation="MVRLLRFGVTLRVGSAAILLLLISFSLASGQVTLLYQKNGTAAG
                     DKLGYSAAGVGDVDGDGKSDFIVGAPFTHPGGRINAGSAYVYSGATGVLLFQIDGVSA
                     GDVLGITVAGAGDVNGDGRSDFIIGIPGRQNYTGSAYVYSGATGALIYQKDGATAGQV
                     FGLAVSGINDLDGDLRADFIVSAGYASPGGVSLAGS"
     gene            1446..1646
                     /gene="rpsU"
                     /locus_tag="AC16GM_05830"
     CDS             1446..1646
                     /gene="rpsU"
                     /locus_tag="AC16GM_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR2923232.1"
                     /translation="MTGVRVRDDETFEKALRRFSKSCEKAGLMADIKKHQHFEKPSER
                     RKRKLAAARRKTRRMRYLDEAA"
     gene            1643..>1909
                     /locus_tag="AC16GM_05835"
     CDS             1643..>1909
                     /locus_tag="AC16GM_05835"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR2923233.1"
                     /translation="MIREKIEADVKVAQKAGEKLKLETLRLLKSDIRYKEIEKRQTLS
                     DDELVAVLSTAAKRRRESIEEFKKGGRADLVAKEEAELGFILGYL"
BASE COUNT          485 a          538 c          515 g          371 t
ORIGIN      
        1 ccgatcctgc taaggagact ccccctgggc tggcatagcc cgcgctgacg ataaaatcag
       61 ccctcaagtc accatccaga tcgttaattc cggacactgc aagaccaaag acctgcccag
      121 ctgtggcgcc atctttctgg taaatcagtg cccctgtggc accagaatac acgtaggccg
      181 agccggtgta gttctgcctc ccgggtatcc cgataatgaa gtcagacctg ccatcaccat
      241 tcacatcccc ggctccagcc accgtgatgc cgagaacgtc cccggccgag acaccgtcta
      301 tttggaacag gagaactccg gtcgccccgg agtagacgta ggccgagcca gcgttgatac
      361 gcccaccggg gtgagtgaaa ggagcgccta caatgaaatc tgatttgccg tcaccatcca
      421 catcaccgac ccccgctgca gaatacccaa gcttgtcacc agcggcagta ccgtttttct
      481 ggtagagcag cgtaacttgc cccgaagcca atgaaaagga aattagcaac agaagaatcg
      541 ccgcgctccc cacccggagc gtgacaccaa aacggagtaa ccgaaccatt tatcctcccc
      601 ggcacatctg cgccgagaaa accctatctg ttaatcaaca tcactttgtt tccttgtcaa
      661 gcaattttcg cgcccttggg tagtttttcc ctcccctgcg agggtcaaag accccgccag
      721 aggcggggga gggtgaggga ggggtcaatg ctctttctct cgcgtaggcg ggtcgcagac
      781 tcgtaccgag actttagtcc cgcatagata catgaggtac tccgactcgt ctctgagccg
      841 gtcggagacg caaatctgct tcatctgctg tgagatgatt cttttcccct aatctctaat
      901 cctcgtaaga gtcgcatcga ccccatttct ccgaatttgt gattgtcgcg gaggcacaca
      961 ggtgcccctt cccgcgctaa tttggagttg ccttgggggt aaaacgaccc cgctatcccc
     1021 ataggcgagg cttccagcct cgcagaaatt tggggtcgct ccgggggtaa aacggacaaa
     1081 gtgcgatata gtaggaggac tgatacaagg cgactggcct ccggcatgct gaggcctcgg
     1141 taaaaaaata aagcatcacg cgccagcgcc cgcagtcctc cgccaggaga aaaaggccac
     1201 gtggccgccc tggaaaatcc agggtaaaaa gtcaaaccgg aaccgatgcg gaaaggggaa
     1261 ataagttcag catctcttcc gcccggcaac cttcccggct aatcttttgt gtcagaatgg
     1321 gatagcacct tccggcactg gggagtggct ctttattgct tgacaaagta aatatccggc
     1381 ccttatcttg ccgatagtga gaatatgacc gtaaacctta accgtgcgaa gggagggtga
     1441 gatgcatgac gggagttcgg gtccgtgacg acgagacctt cgaaaaagcg ctgcgtcgct
     1501 ttagcaagag ttgtgaaaaa gccggcctga tggctgatat aaaaaagcac cagcacttcg
     1561 aaaaaccgtc cgagcgccgc aaaaggaaat tggccgcggc gcgccgcaaa actcggcgga
     1621 tgcgctacct cgacgaggcg gcatgatccg ggagaagatc gaggccgatg taaaagtcgc
     1681 gcagaaagcc ggagaaaaac tgaaattaga gacgctgcgg cttttgaaat cggacatccg
     1741 ctacaaagaa attgagaaaa gacagaccct ttcggatgac gaactggtcg ccgtgctctc
     1801 cacggcggcc aaaaggcgcc gggagtcgat cgaagagttc aaaaaaggcg gacgggcaga
     1861 cttagtagcc aaggaggaag ccgaattggg cttcattctt ggctacttg
//
LOCUS       DBNGZD010000223         4387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1215952, whole genome shotgun sequence.
ACCESSION   DBNGZD010000223 DBNGZD010000000
VERSION     DBNGZD010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1215952"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16GM_05840"
     CDS             <1..189
                     /locus_tag="AC16GM_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923234.1"
                     /translation="RVGSFQSGRLAVLQGQFVDLYQKIQRAKHQIALLLRQSPLGQTT
                     TKLTPPSMPIPPRHFAAQ"
     gene            301..2004
                     /locus_tag="AC16GM_05845"
     CDS             301..2004
                     /locus_tag="AC16GM_05845"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR2923235.1"
                     /translation="MRIFTAPNFLLYLPVILLSVPLSGMAQKQVDLLLQNGRVATLDT
                     QQPWAEAVAMAGDSILAVGATVELEKFSGPRTKVIDLGGRLVIPGFNDAHCHLLSGGL
                     SLLRVNLLGATTAEEIKRRVAEKVRESPPGSWIRGRGWDHTLFNRGRWPDKSLLDSVA
                     DGHPIFLMRVDGHVGWANSEAFRLAGITSATPDPPGGEIVRDPETKEPTGIFKENAVD
                     LINTVVPPPTKTELTLALEKALVEARRLGVTSVQEQSDPGDFPGGYEVYREFFDAGKL
                     TLRIDTWLALNDATKPHRLDSLQALFPKAGGWLRAGMLKGFTDGSMGSRTALFFAPYS
                     DDPTTSGIAGYTQDSLNNLVRIGDSLGYQIGLHAIGDKANYMALKAFDFARLTNGPKT
                     RRHRLEHAQVMRPQDIEYCARLGVVALMQPTHCTSDMRWAEDRVGKERCKGAYAWRSF
                     LDNRVPLAFGTDWPVEPLDPMEGIYSAVTRQPREVVKPLPESWMPEQRITVEEAVRAY
                     TFGSAYAEFQEEKKGSIAPGKLADLVVLSQNIFTIPPREILSTKVDLTIVGGKVVFER
                     K"
     gene            complement(2207..2395)
                     /gene="rpmB"
                     /locus_tag="AC16GM_05850"
     CDS             complement(2207..2395)
                     /gene="rpmB"
                     /locus_tag="AC16GM_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR2923236.1"
                     /translation="MARICDICGKKPSVGYTVSHANKRSKRTWQPNLQMVRAKVNGKP
                     TRLRVCTGCIGAGKVTKA"
     gene            2597..>4387
                     /gene="recG"
                     /locus_tag="AC16GM_05855"
     CDS             2597..>4387
                     /gene="recG"
                     /locus_tag="AC16GM_05855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR2923237.1"
                     /translation="MENSPALKSKDQPAPRKAALKLDTPIQYVKGVGPARAKILAKVG
                     IQAVGDLLYYIPRRYLDRSNIRPIGQLSAVPPDGNRSEEMTVIGKVAAAGILKGRKNR
                     FEVLLKDDTGYLALLWFNWPNYLPKYLKKDMELAVSGQVTYYHGYQMIHPDFEVLSGD
                     GEDELLHTGRIIPLYPSTTDLKAHFLDSRGLRRLVAETLPAVRPVIVDFIPPEVLADV
                     GCPPLDVALNQVHFPDSLADADLARRRLAFDELFFLQLLLQARRATTKKEAKPRTFKP
                     PGDLVWRFLRQLPFQFTAAQKKVFAEITADLTSPAIMNRLLQGDVGSGKTVVAVAAML
                     GAVENGYQAALMVPTEILAEQHYFTLSRRLEQIGVSTALLTGSTPEKEKKEMHKQLAA
                     GKIQIVFGTHALLQEKVTFRDLALVVIDEQHRFGVLQRAGLRKKGFLPELLVMTATPI
                     PRTLSMTLYGDLDVSVIDEMPPGRQKITTVWRQEPARPDILKFIADQVRKGFQAYIIY
                     PLVEESEKADLKAATESYHELQEKHFSDFNVGLIHGRLKAEERTRVMEAFRKREYQIL
                     VSTTVVEVGMDIPNATVMLVEHAERFGLSQL"
BASE COUNT          932 a         1255 c         1256 g          944 t
ORIGIN      
        1 cgggtgggca gtttccagtc gggccggctg gccgtactgc aaggacagtt cgtggatctc
       61 taccagaaga ttcagcgggc caagcatcaa attgcccttt tgctccggca atcgcctctg
      121 ggccaaacca cgacaaaact gaccccaccc tcgatgccca taccccctcg tcattttgcc
      181 gcgcaatagc gttggcttga attgtacccg attcagacgg tacgtcttct cccgcaactt
      241 gtcttaggca gaaaagacac tatttttact tccgtgtgcc caaaagctat cgtccgacga
      301 ttgcggatat tcaccgctcc taactttctc ctctatctcc ctgtgattct cctttccgtt
      361 ccactttccg gaatggcgca aaaacaggtc gacctgcttt tgcagaacgg ccgggtggcc
      421 acgctagata cgcagcagcc ctgggccgag gcggtggcta tggccgggga ttcgattctc
      481 gcagttggtg ctaccgtcga actcgaaaag ttttcggggc cgcggacaaa ggtaatcgat
      541 ctgggcggtc ggcttgttat tcccggcttc aacgacgccc actgccatct cttgagcggc
      601 gggctgtcgc ttttgcgggt caatctcctc ggcgccacta cggctgaaga gatcaagcgt
      661 cgcgtcgccg agaaagtgcg ggaatcaccg ccggggagtt ggattcgcgg gcgggggtgg
      721 gaccacaccc tcttcaaccg gggccggtgg ccggataagt cgctccttga ttcggtcgcc
      781 gacggtcatc ccattttcct catgcgggtg gatggccacg tgggctgggc aaattccgaa
      841 gcgttcaggt tagccggaat cacgagcgca acgccggatc cgccgggcgg ggaaattgta
      901 cgcgatccgg aaaccaaaga gccgaccggc attttcaagg aaaatgcagt tgatttgata
      961 aatacggtcg ttccgccgcc gacgaagacc gaattgacgc tggcgttgga gaaagcgctc
     1021 gtggaagcac ggcggctggg cgttacttcg gtgcaggagc agtccgaccc gggagatttc
     1081 cccggcgggt atgaagtcta ccgcgaattt ttcgacgccg ggaaattaac cttacgcatc
     1141 gatacttggc tggccctcaa cgacgcgacc aaaccgcatc ggctcgattc gctgcaagcc
     1201 cttttcccga aagcaggcgg ttggttgcgg gccggcatgc tcaaggggtt caccgacggg
     1261 tcgatgggct cgcgcacggc gcttttcttt gcgccttact ccgacgaccc cactacctcc
     1321 ggcattgcgg gctatacgca agattcgctg aacaacttgg tgcgcattgg cgacagcctg
     1381 ggctaccaga tcggcctgca cgccatcggc gataaggcca attacatggc gctgaaagca
     1441 tttgatttcg cccggttgac gaacggacca aaaacgcgcc ggcaccggtt agaacatgcg
     1501 caggtgatgc gcccgcagga tatcgaatac tgcgcccggt taggcgtggt ggccttgatg
     1561 cagccgaccc actgcacttc cgacatgcgc tgggctgaag accgggtggg gaaagaacgg
     1621 tgcaaaggcg cctacgcctg gcgcagtttc ctggataata gggttcccct ggcctttggc
     1681 accgactggc cggtggaacc gctcgacccg atggaaggaa tttactccgc ggtcacccgc
     1741 cagccgcgcg aggttgtcaa gcccttgccc gaaagctgga tgcccgaaca gcgcatcacc
     1801 gttgaagaag cggtgcgagc gtatactttt ggttcggcgt acgcagaatt tcaggaagag
     1861 aaaaaaggct cgattgcgcc ggggaaactg gccgatctgg tcgtgctctc tcagaacatt
     1921 ttcaccatcc cgccgcggga gattttgagc accaaagttg atctgacgat agtggggggg
     1981 aaggtggtgt ttgagaggaa gtaaagagat gttggctttc taaatgacct caccctgccc
     2041 tctcttacaa ggagagagag ttttttgagg aaagaagaga actggaattt ccctcccctt
     2101 gaggatcgca gactcgtatc aaggagggtg agggaggggt cttcaattct cggtaataga
     2161 aaaagccccg ctggcgggtc ttctcgattc aggcggcagt ctttgtctac gccttggtaa
     2221 ctttccccgc tccgatgcag ccggtacaga cccgcaggcg ggtcggcttg ccgttcacct
     2281 tggcccgcac catttgcagg ttcggctgcc aggtgcgttt gctccgttta ttcgcgtggc
     2341 tgactgtata gccgacactc ggtttcttcc cgcaaatgtc gcagatgcgt gccattggtc
     2401 ctccgttaca ctatcaatat agcgattaag gagcaaaggg caagtccgta attggaaatt
     2461 tcaaatcgtt tccctacccc tcggctttgc tggttcgact ttgctcacca cgagtcggga
     2521 caagccacct atcaccttcc acctacgacc tcttttcttg actctccgaa agcgagcggg
     2581 gtatgttacc ccggcgatgg aaaactcgcc agcattgaag tcgaaagacc agccggctcc
     2641 tcgcaaagcg gcgctgaaac tcgacacgcc gattcagtac gtgaagggag tcggtcccgc
     2701 gcgggcgaag attttggcaa aagtcggcat ccaagccgtc ggtgatctgc tctactacat
     2761 cccccggcgc tatctcgacc gctccaacat tcgccccatc gggcagctca gtgccgtgcc
     2821 gccagatgga aatcgttccg aggaaatgac ggtcatcggc aaggtggccg ccgctggcat
     2881 cctgaaaggg cggaagaatc gctttgaggt actcctgaag gacgatacag ggtatctcgc
     2941 gctcctctgg ttcaactggc cgaactatct gcccaaatat cttaaaaagg acatggaatt
     3001 agcggtctcc ggacaggtta cttactacca cggctaccag atgatccatc ccgatttcga
     3061 ggtgctctcc ggggacgggg aggacgaact cctccacacc ggacggatca tcccgctcta
     3121 tccctccacc acagacctca aggcgcactt tctggacagc cgggggctgc gccggttagt
     3181 ggcggagact ttgcccgcgg tccgtcccgt catcgtcgat ttcatccccc cggaggtttt
     3241 agccgacgtc ggctgcccgc ctctggatgt cgcgctcaat caggtgcact tccccgattc
     3301 gttagctgat gccgatttgg cccgcaggcg gctggctttc gatgaactat tcttcctgca
     3361 actgctcctg caggcgcggc gagccacgac caagaaggaa gccaaaccga gaacgttcaa
     3421 accaccaggc gatttggtct ggcggtttct gcggcaacta ccctttcaat tcacggcagc
     3481 ccaaaaaaaa gtcttcgctg aaataaccgc cgacctgacc tcacccgcca ttatgaaccg
     3541 tctgctgcag ggggatgtcg gttctgggaa aacagtcgtg gctgtggcgg cgatgctcgg
     3601 tgcggtagag aacggctacc aggccgcctt gatggtcccg accgagattt tggcggaaca
     3661 gcactacttc actctttcgc ggcgtctgga gcagatcggt gtttcgacgg ctttgctcac
     3721 cggctcgacg ccggaaaaag aaaaaaaaga aatgcacaag caattggccg ccggaaaaat
     3781 ccagatcgtt ttcggcacgc acgcgctgct tcaggagaaa gtcacttttc gtgacctggc
     3841 tttagtggtg atcgacgaac agcaccgctt tggtgtgctc cagcgggcgg ggctgcgcaa
     3901 gaaaggattc ctgccggaac ttttagttat gaccgctacg ccaattccac gcactttatc
     3961 catgaccctc tacggcgact tggacgtctc ggtgattgac gagatgccac cggggcggca
     4021 gaaaatcacc accgtctggc ggcaggaacc ggcccgtccc gacattttga agttcatcgc
     4081 cgatcaggtg cggaaagggt ttcaggcgta catcatctat cccttggttg aagaatctga
     4141 aaaagccgat ctcaaagcgg caacggaaag ttaccacgaa ctgcaggaaa aacatttttc
     4201 tgatttcaat gtcgggctta ttcacgggcg gctcaaagcg gaggagcgga cgcgagtcat
     4261 ggaagctttc cgcaagcggg agtatcaaat cctcgtctcc acgacggtgg tggaagtggg
     4321 gatggacatt cccaacgcca cggtaatgtt agtggagcat gccgagcgtt tcggtttgtc
     4381 gcaattg
//
LOCUS       DBNGZD010000224         1883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_799731, whole genome shotgun sequence.
ACCESSION   DBNGZD010000224 DBNGZD010000000
VERSION     DBNGZD010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_799731"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="AC16GM_05860"
     CDS             <1..487
                     /locus_tag="AC16GM_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR2923238.1"
                     /translation="YYRKAYYRSFFWDPPACAIGEVWKGTYHGETRFPFILQNLHRYF
                     FYAAVVVLAFLWYDTIKAFIFDGRFGIRIGSLLYLVNIILLSAYTFSCHSFRHLIGGQ
                     LDCYSCSASAGARHSLWEKVSLLNHRHMLWAWLSLFSVGLTDLYVRLLATGVVRDRII
                     F"
     gene            487..>1883
                     /locus_tag="AC16GM_05865"
     CDS             487..>1883
                     /locus_tag="AC16GM_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR2923239.1"
                     /translation="MEAYETVTHDILVIGAGGAGLRAAIAAREQGLSVGLVCKSLLGK
                     AHTVMAEGGVAAALGNVDPQDNWQVHFADTLRGGQFLNNWRMVEIFAREAPERVLELE
                     QWGAAFDRTPDGKIMQRPFGAHTYRRLSHVGDRTGLEMIRTLQDKAVASGVEVYMEVT
                     CTRLVKDGDRVVGAFGYRRQNGRFVLFKARAVILATGGAGKLYKVTSNSWESTGDGVG
                     MAYEAGAELIDIEMVQFHPTGMVWPSGVRGILVTEGVRGEGGILKNNKGERFMQRYDP
                     KKMDLSSRDVVSRAIYTEVQEGRGTEHGGVYLDITHRGADYIKKKLPSMYDQFLALAD
                     VDITREPMEVAPTIHYAMGGVRVDPETGASSLPGLFAAGEVAAGLHGANRLGGNSLSD
                     LIVFGKRAGVGAAVYVKSQPVSTRMAEAQMQAEADLLLQPLQNPGKENPYQLQAELQE
                     TLQNYVGIARNEADL"
BASE COUNT          357 a          566 c          569 g          391 t
ORIGIN      
        1 ctactaccgc aaagcgtact atcgttcgtt cttctgggac cctccggcct gtgcgatcgg
       61 cgaagtgtgg aaggggacgt accacggtga aactcgcttt cccttcattc tccagaacct
      121 ccaccgctac ttcttctacg ccgccgtcgt cgtcctggcc ttcctctggt acgatacgat
      181 caaggctttc atcttcgacg gtcgcttcgg cattcggatc gggtcactgc tctatttagt
      241 gaacataatc ctgctctccg cctacacttt ctcctgtcat tcgttccgtc atttgatcgg
      301 cgggcaactc gattgctact cttgctcggc ttccgccggc gcccgacact ccctctggga
      361 aaaagtgagt ctcctcaatc accgccatat gctctgggcc tggctttccc ttttttcggt
      421 gggcttgact gatttatatg tccggctcct ggccaccggc gtcgttcgtg atcggataat
      481 cttctgatgg aagcgtacga aaccgtcacc cacgacattc tggtaattgg cgccggcgga
      541 gccggcctgc gggcggccat tgctgcccgg gagcagggtc tttcggtcgg gttggtctgc
      601 aagtcgctat tgggcaaggc gcacaccgtg atggccgaag gcggcgtggc cgcggccctg
      661 ggtaacgttg atccgcagga caactggcag gtccactttg ctgacacgct gcgcggcggc
      721 cagttcctca acaactggcg gatggtggaa atctttgcca gagaagcgcc cgagcgggtg
      781 ctggaattag agcagtgggg cgcggcgttc gatcgaactc ccgacggcaa gatcatgcag
      841 cgccccttcg gcgcccacac ctaccgacgc ctctcccacg tcggcgaccg caccggcttg
      901 gagatgatcc ggaccctgca ggacaaagcc gtggcctcag gcgtcgaagt ttacatggag
      961 gtcacctgca cccgattagt gaaagatggt gaccgggtgg tcggcgcttt cggctaccgc
     1021 cgccagaacg ggcgcttcgt tctcttcaaa gcgagagcgg tgattttggc taccggcggg
     1081 gcgggcaaac tgtacaaggt gacctcgaac tcctgggaga gtaccggcga cggcgtcggc
     1141 atggcgtacg aggccggggc cgaattaatt gacatcgaaa tggtgcaatt ccaccccacc
     1201 ggtatggtct ggccctcggg ggtgcgcggg attctggtca ccgaaggcgt ccgcggcgag
     1261 ggcggtatct tgaaaaacaa taagggcgag cgcttcatgc agcgctacga tccaaagaaa
     1321 atggacctct cctcacgcga tgtggtctcc cgggcaattt acactgaagt gcaggaaggg
     1381 cgcggcaccg agcacggcgg cgtctatctg gacatcaccc accgcggcgc cgactacatc
     1441 aagaagaaat tgcccagcat gtacgatcag ttcctggcct tagccgacgt cgacattacc
     1501 cgagagccga tggaagtggc gccgacgatt cactatgcca tgggcggggt gcgggttgat
     1561 cctgaaaccg gcgccagttc cctaccggga ctttttgccg ccggcgaagt ggccgccgga
     1621 ctccacggcg ccaaccggct gggtggaaat tccctctccg acctgattgt cttcggcaaa
     1681 cgggcgggcg ttggggcggc tgtttacgtg aagtcacagc ccgtttctac ccgtatggct
     1741 gaggcgcaaa tgcaagcgga agctgacctg cttttgcagc cgttgcagaa cccggggaag
     1801 gagaatccgt accagctgca agccgaactg caggagacgc tgcagaacta cgtcggcatt
     1861 gcccggaatg aagctgacct caa
//
LOCUS       DBNGZD010000225         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_938692, whole genome shotgun sequence.
ACCESSION   DBNGZD010000225 DBNGZD010000000
VERSION     DBNGZD010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_938692"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..800)
                     /locus_tag="AC16GM_05870"
     CDS             complement(117..800)
                     /locus_tag="AC16GM_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923240.1"
                     /translation="MGTATATAAAPTTKRTILPAASMGMNDSDPQAIRRARTEEARGF
                     FDFIAQDFPPLSTTREAVEKRLQAGWLLDYVPSGLTFKKPFYDIRNFYSLSLWFLKSK
                     PDPDSPPYADPPTPGRWLFYPREMFIPGSGLKIKPEQEVFLKEKFPGGKWVWPEAGEI
                     TYLLTLNQKRTGEYLLETEWVRTNTPCGANNEYTIAVGFHHFDNLIVGWTKPTYRAPD
                     LFACPVIPA"
     gene            complement(948..1313)
                     /locus_tag="AC16GM_05875"
     CDS             complement(948..1313)
                     /locus_tag="AC16GM_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923241.1"
                     /translation="MQRKVFSLVIAVLVLGGFVAGPSRAQLPPPVCKQALEEVQKTNQ
                     MLQRAAPVIRDCPNDTAKFLFRRAVQVQGAAIMALRSRHCVLALDLTTHARRVALKAL
                     SFCGPPGEALPPSGADPGE"
     gene            complement(1401..>2820)
                     /locus_tag="AC16GM_05880"
     CDS             complement(1401..>2820)
                     /locus_tag="AC16GM_05880"
                     /inference="COORDINATES: protein motif:HMM:NF017183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2923242.1"
                     /translation="CLVLIGLLSLPPAVGEANDSVTVRILTGSHTIQVTTLTFTIPGM
                     GMVSVSPLPPVQGPGDSTFGFYAVADTPTAVSMPFLMDGVGHSFSANPLRYDTLYTLP
                     FHGSPDSKIMFRVRHPDNPPVLAAIGSKNVMEGQMLQFVVTATDPDPSPTLILSALNR
                     PPNAAFVDSGNGHGLFTFNPDFNQAGMHPVLFVVSDGQKADSEQVTIQVQDVDRPPVL
                     AATGLQTVAEGQVLQVPISASDPDAEPLALIAQNLPVHATLTDSGGGRGLLRFAPDFD
                     QAGPYQVTIIARETRAIGPLADTGAVNINVTNTNRPPVLAAIGPQAIAEDDTLLFAVN
                     ALDPDGQSVTLAAFNLPPNALFVDSLNGRGSFRFTPDTTQQGVYDVLFTATDGTASDS
                     QTVSISVCDISAAPVLADVNCDGVVDITDIVFLINYVVFGAPAPVRAGTADVNCDLVV
                     DLVDIIATIQNVVFGTPFTGCP"
BASE COUNT          588 a          776 c          897 g          559 t
ORIGIN      
        1 gattgtaggg gcaattcatg aattgcccct acggaccgac ggtcagagac cgtcgctaca
       61 acgtggatgg aatgtctttg taggggttca atgcattgaa cccctacaat tgcgtttcag
      121 gcgggaatca ccgggcaggc gaacaaatcg ggagcccggt aggtgggctt tgtccagccg
      181 acgatcaggt tatcgaagtg gtggaaaccg accgcaatcg tatactcgtt attcgcgccg
      241 cagggggtat tcgtacgcac ccactcggtt tccagcaggt attcgccggt gcgtttctgg
      301 ttcaaggtga ggaggtaggt aatctccccc gcttcgggcc agacccattt tcccccgggg
      361 aacttctcct tcaggaagac ctcctgctcc ggtttgattt tcaatccgct tcccggaatg
      421 aacatttccc gcgggtaaaa gagccagcgg cccggagtgg gcgggtcggc gtacggcggg
      481 gagtcggggt cgggcttgct cttcaaaaac cagagggaaa gcgaatagaa gttccggatg
      541 tcgtagaacg gctttttgaa ggtcaatccg gacgggacgt agtcgagaag ccagcccgcc
      601 tgcaggcgct tttcgaccgc ctcgcgggtt gtggaaagcg gaggaaaatc ctgcgcaatg
      661 aagtcaaaaa aaccccgggc ctcttcggtg cgcgcccggc gaatcgcctg cggatcggaa
      721 tcgttcatcc ccattgacgc tgctggcagg atcgtccttt tggttgtcgg cgcagcagca
      781 gtagcagtcg ctgttcccat aagtgctccg tgtttaccga taacgcttat ttcggcctct
      841 cagacggttt tatatagttt gccgggaggt ggttgtcaag ccaacgaaaa gcgtcccgcc
      901 gtagtttgac gggacgcttt tatcacattt ccttgaggaa cgaacgatta ttctccggga
      961 tccgcgcctg acggcggaag tgcctctccg ggcggtccgc agaaagataa ggctttgagc
     1021 gccacgcgcc gggcatgcgt ggtcaaatcg agcgccagaa cacagtgacg gctgcgcaag
     1081 gccataatcg cggcaccctg cacctgcacc gccctgcgga acaggaattt ggccgtgtcg
     1141 ttggggcaat cccgaatcac cggcgccgcc cgctggagca tttgattggt cttctgtact
     1201 tcctccagcg cctgcttgca gaccggcggc ggcagctggg cccggctggg gccggccaca
     1261 aaacccccga ggacgagtac ggctattacc agcgaaaaca ccttcctttg catcctcatc
     1321 acctccctga tctgaaatgg ttgcgtttca ctgtctacag tatttcagac caaaggagcg
     1381 aaggaaaggt ttaagcgttt ttacggacag ccggtaaaag gcgttccaaa aaccacgttc
     1441 tggatggtgg cgatgatgtc gaccagatca acgactaagt cacagttgac gtcggcggtt
     1501 ccggcccgga ccggcgccgg agcgccaaag acgacgtagt tgatgaggaa gacaatgtcg
     1561 gtaatgtcga cgacgccgtc gcagttgacg tcggccagaa ccggcgcggc ggaaatgtcg
     1621 cagactgaaa tcgaaaccgt ttggctgtcg gacgcggtgc cgtcagtcgc ggtgaagagc
     1681 acgtcgtaaa ccccctgctg ggtcgtatcc ggggtgaagc ggaacgagcc ccggccgttg
     1741 agactatcga cgaacagggc gttgggaggc agattgaacg cggcgagagt gacgctctgg
     1801 ccgtcggggt caagggcgtt cacggcaaaa aggagcgtgt cgtcttcggc aatcgcctgg
     1861 gggccgatgg cggcaagcac cggggggcgg ttggtgttcg ttacgttgat gttcaccgct
     1921 ccggtatcgg ccaaaggtcc tatggctctg gtttctcggg cgatgatggt gacctggtac
     1981 gggccggcct gatcaaaatc gggagcgaaa cgcaagagcc cgcggcctcc gccgctgtcg
     2041 gtcagggtcg cgtgcaccgg gagattctgt gcaattaagg ccagcggctc ggcatcggga
     2101 tcgctggcgc ttatcggaac ctgcaacacc tgcccttcgg ccactgtctg aaggcctgta
     2161 gccgccagaa ccggcggacg gtcgacatcc tgaacctgaa ttgtcacctg ttcgctgtcg
     2221 gctttttggc cgtcagaaac cacgaacagc accgggtgca tccccgcctg attgaaatcg
     2281 ggattgaagg tgaaaaggcc gtgcccgtta ccgctatcga caaacgccgc attcggcggc
     2341 cggttgaggg cggagaggat aagggtcggg cttgggtcgg gatcggtggc cgtcacgacg
     2401 aactggagca tttgtccttc catgacgttt ttcgacccga tcgccgccaa aaccggcgga
     2461 ttgtctggat gcctcacccg gaacataatt tttgagtcgg gactgccgtg gaagggcagg
     2521 gtgtagaggg tatcgtacct gagcggattg gcggaaaagg aatgacccac cccgtccata
     2581 aggaagggca tactcaccgc cgtgggcgta tcggcgacgg cgtagaagcc gaaggtggaa
     2641 tcgccgggcc cctgcaccgg cggcaaaggc gacaccgaca ccatccccat tccggggatg
     2701 gtgaaggtaa gcgtggtaac ctgaatcgta tgcgaaccgg tcaggatgcg cacggtaacg
     2761 ctgtcattgg cttccccaac cgctggcgga agcgagagga gtccaatcag gacaaggcaa
//
LOCUS       DBNGZD010000226         1494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1633759, whole genome shotgun sequence.
ACCESSION   DBNGZD010000226 DBNGZD010000000
VERSION     DBNGZD010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1633759"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1281)
                     /locus_tag="AC16GM_05885"
     CDS             complement(82..1281)
                     /locus_tag="AC16GM_05885"
                     /inference="COORDINATES: protein motif:HMM:NF025104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR2923243.1"
                     /translation="MSGGGKFQPGQKQSGATVITQRLQLFRRLALAGDLFLLFLVYLL
                     VVAFRESTAVQQLLNLQEYSPYRPMLLLILPIWGLVLALSPHCYELRAKRLWRMGLDL
                     GVVTLKGAGVLLAALFVLHAPPQSRFLVFLFCTTSWLALVTYRVTLTTALGWARRRGK
                     NLQKLIVVGTGRAAQKLVDQILTHPAWGLRISGFVYNGEDLSLWRFRDIPVIGTLFDL
                     PRIIQNNHIDWVVFAVPEGQLGLIGPSMQLCEEMGTNVCLLTDFFSLRFARKKAGELF
                     GQPVLLFSRAPEPDVRLFIKYCLDRLLAFLGLVLVAPIMACAAVVIKLASPGPVLFRQ
                     ERCGLNGKRFTLYKFRTMVPEAEALKSQLLDKNEMDGAAFKITNDPRLTPVGKVLRKY
                     SLECHNL"
BASE COUNT          351 a          449 c          415 g          279 t
ORIGIN      
        1 cgtatccaca ctttgtttgt ttcagatgcc gacctccatg gcgatccgtc tgcgctctgc
       61 gcagaactcg ccagccgcgg actaaaggtt gtgacattcg agggaatatt tccgcagcac
      121 ttttcccacc ggggtgagcc gtgggtcgtt ggtgattttg aaagccgctc cgtccatttc
      181 gttcttatcc aaaagctgac ttttcaacgc ctcggcctcc ggcaccatgg tgcggaattt
      241 gtagagagta aagcgcttgc cgttcaaccc gcagcgctcc tgccggaaga gcaccggccc
      301 gggagacgca agcttgatga caaccgccgc gcaggccata atcggagcca ccaataccaa
      361 ccccagaaaa gcgaggagcc ggtcgaggca gtacttgatg aagagccgaa cgtccggttc
      421 gggcgcccgg gagaagagga gcaccggctg gccgaacagt tcccccgctt ttttgcgggc
      481 gaagcgcagg gagaaaaaat cggtcaaaag acagacgttc gtccccatct cttcgcagag
      541 ctgcatgctg gggccgatca atcccaactg cccctccggc accgcgaaga ccacccagtc
      601 gatgtggttg ttctgaatga tacggggcag gtcaaagagg gtgccgatga ccggaatgtc
      661 acggaagcgc cacaacgaga ggtcttcccc gttgtagacg aatcccgaaa tgcgcagccc
      721 ccaggccgga tgggtgagga tctggtccac caatttctgc gccgcccgtc cggtaccgac
      781 gacgatcaat ttttggagat tctttccgcg cctccgcgcc caccccaagg cagttgtcag
      841 ggtcacgcgg taggttacca gggccaacca actggtggta cagaagagaa ataccaaaaa
      901 gcggctttgc ggcggcgcat gcaaaacgaa aagtgcagcg agcaggacac ctgctccctt
      961 gagcgttacc acacccaggt ccagccccat ccgccagagt cgcttggccc gcagctcgta
     1021 gcagtgcggc gacaaggcca aaaccaaccc ccagatcggc aggatgagca aaagcatcgg
     1081 ccggtaggga ctgtactcct gaagatttag cagttgctga acggcagtag attcacgaaa
     1141 ggccaccacc aagaggtaca ccagaaacaa gaggaaaaga tcgcccgcca gggcgagacg
     1201 gcgaaagagc tgtaaccttt gcgtgatcac agtcgccccg ctctgtttct gtcccggctg
     1261 gaatttgcct ccgccggaca agcagttccg cttgcttgaa ataccatttc tctcattgcc
     1321 tcactccctg aagcttccta ttcccttatt cggcaagaag atggaagact aaaggggcac
     1381 agaacctcct gcccggcatc gaaacgacct tctctcaggc ggagcaacga atcgacggag
     1441 aaagacgctg gactggccgg cctttgcatc ccgcccgtgg ggattttttc aatt
//
LOCUS       DBNGZD010000227         2551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1911227, whole genome shotgun sequence.
ACCESSION   DBNGZD010000227 DBNGZD010000000
VERSION     DBNGZD010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1911227"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1337)
                     /locus_tag="AC16GM_05890"
     CDS             complement(66..1337)
                     /locus_tag="AC16GM_05890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR2923244.1"
                     /translation="MKRKRCFLIVFVVLVVGLYGIKVCHKRGDLPVFLQGARNLWTGH
                     QIYYDVPGAFTYPPFFALFVSPFALFPLPVATVLWYLFNVACILGCLLLLRNMILTRD
                     AESKTVNPVLVIGLGFLFTVRFIFDAFENQQFDLVVFCATLLFLLFLSREKPVRSGFF
                     MALAGSLKVTPLLFIVYLLFNRKVNVLAWTFVFLLTLNSVPEIFGLVRGRLYAAEWAE
                     VILKRVNPASGGRPWASGGVWQSSSVFNQSLSAMFYRYFTDVPVEGMKYGSVRVNFAS
                     LDPGRIKLLTYLAYGILFAVTLFVSRPVNSIGGQAQLYEWSLFLLLMVLVSPISSKAH
                     FVVLFLPNLLLWRERLLKKLPAALTVLFFLSFGLTTLTADGLIGRKLADLASAFSCVT
                     LGTLVLWIAVVVRRYQMAGTGRTAGIAPMAI"
     gene            complement(1334..>2551)
                     /locus_tag="AC16GM_05895"
     CDS             complement(1334..>2551)
                     /locus_tag="AC16GM_05895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2923245.1"
                     /translation="CTKCSSVMPVEVETTATVARDAFSQRRNTDQLLLNVFFLNTIAA
                     EVWGGGEEVFFQWATGLAARGHRVTAAGRLKSLFLDRFKGTDIDIVTLPIGGDFDPRV
                     VWRLSDLFRKKEIDLVCVNFNKGLRLAGLAARWVGRPAVVWVQGVKLPKNRWHHRWLT
                     NRLVDRIIVPSQSLRRELEQFPWLDQRKISVIANGIDLRRFADLTEEGQRVRQQLGVA
                     KSELLIGIFGRLIEAKGHKIFLAAFSELIKKNAGLKTVVVGSGLLESELKNLVTARGL
                     SASVILAGFQKKPGAFMAACDLIVHPALYEPFGLVLLEAMALGKPVVASRVGGIPEVI
                     TDGETGLLVPPGDVMALVAAIMQYIENPQLCLEFGARGRRWVEEHFQLDDIMGQVEAT
                     LVQIARERREKVG"
BASE COUNT          688 a          739 c          654 g          470 t
ORIGIN      
        1 ggaaaattcc accatatacc aggagccggc gttccagccg tctaagacga ttgattgcag
       61 aattctcata tggccatcgg ggcaatgccg gccgtacgcc cggtgcccgc catttggtac
      121 cgacggacca ctacggcgat ccagagcacg agagtcccga gcgtgacaca ggagaaagcg
      181 ctggcgagat cggccagttt tctcccgatc agtccgtcgg cagtcaacgt agtaagtccg
      241 aaagaaagaa aaaagagtac ggtcaaggcc gccggtaatt tcttgagcag ccgctcccgc
      301 cagaggagca gattcggcaa gaacagcacc acaaagtgcg ccttgctgga tatcggggag
      361 accagcacca ttaaaagcaa aaacaaactc cattcataca gttgcgcttg accaccgatt
      421 gaattcaccg gccgcgaaac gaaaagggtt accgcgaaca aaataccata agcaagatag
      481 gtcaaaagtt ttatccgtcc cgggtcgaga gaggcaaagt tgactctgac cgacccgtat
      541 ttcatcccct ccaccggcac atcagtgaaa tatcggtaga acatggccga aagcgattga
      601 ttgaacaccg aacttgactg ccagacaccg ccggaggccc agggtctccc gccggaagca
      661 ggattcaccc gcttcaatat tacctccgcc cattcggcag cgtagagccg gccgcgcacc
      721 agaccgaaaa tttccggcac cgaattcagt gtcaggagga aaacaaaggt ccaggccaaa
      781 acgttcactt tccgattgaa aagcaggtag acgataaaaa ggagcggcgt gactttcagt
      841 gaaccggcca gcgccatgaa aaatccagac cggaccggct tctcacgaga gaggaacaat
      901 aaaaacagaa gggttgcgca gaagacgaca aggtcgaact gctgattctc gaaagcgtcg
      961 aaaataaaac ggacggtgaa gagaaacccc agacctatga ccagaacggg attgaccgtc
     1021 ttgctttcgg catctcgtgt cagaatcatg ttgcggagca aaaggagaca acccaagata
     1081 caggcgacat tgaacaggta ccaaagcacc gtggcgaccg gcaacgggaa aagcgcaaaa
     1141 ggtgaaacaa aaagggcaaa gaatggcggg taggtgaaag cacccggcac gtcatagtaa
     1201 atctggtgtc ccgtccagag gtttcgggcg ccctgcaaaa agacgggaag atcgccccgc
     1261 ttgtggcaga ccttgattcc atagaggcct actacaagga cgacgaagac aatcaaaaaa
     1321 cagcgttttc tcttcatccg accttttccc gccgctcccg cgcgatctgc acgagcgtcg
     1381 cctcaacttg ccccataatg tcatccaatt gaaaatgctc ctccacccat cttcttcccc
     1441 gggctccgaa ctcaaggcac agttgaggat tttcgatgta ttgcattatc gctgcgacga
     1501 gagccatcac atccccgggc ggcacgagca aacccgtttc gccgtcggtg atcacctcag
     1561 gaatgccgcc cacccggctc gccaccaccg gcttgcccaa ggccatcgct tcgagcagaa
     1621 ccaacccaaa aggttcgtag agagcaggat gaacaataag gtcacaagca gccatgaacg
     1681 ctccgggttt tttttgaaaa ccggccaaaa ttacgctggc cgaaagcccc ctcgctgtca
     1741 ccaggttttt taactcagac tcgagtagcc cggaaccgac tacaaccgtt ttgagtccgg
     1801 cgtttttttt gatgagctca ctgaacgccg ccaggaaaat cttatgcccc ttggcttcaa
     1861 tcagccgacc gaaaatgccg atgagcaact cacttttggc tactcccagc tgctgtcgca
     1921 ccctttgccc ttcttcagtg aggtcagcaa atcggcggag atcaatcccg ttggcaatca
     1981 cggagatttt tcgctggtca agccagggga actgttcgag ctccctgcgc aaggattgcg
     2041 atggcacaat gatgcggtcc accaaacgat tcgtcagcca gcgatgatgc cagcggttct
     2101 tgggtaactt taccccttgc acccagacca ccgccgggcg gccaacccac cgcgctgcca
     2161 gcccggccag gcgcagacct ttgttgaagt tgacgcagac cagatcaatt tccttcttgc
     2221 ggaagagatc ggagagcctc cagacgactc gcggatcaaa atcaccgcca atcggaagag
     2281 taacgatatc gatgtccgtg cctttgaacc ggtcgagaaa aagacttttc agacggccgg
     2341 cggcggtaac gcgatggccg cgcgccgcca agccggtggc ccactggaaa aaaacctctt
     2401 ccccaccgcc ccagacttca gcggcgatgg tgttcaaaaa aaatacgttc aacagcagtt
     2461 ggtcggtgtt ccgccgttgg gaaaaagcat ccctcgcgac ggttgcggta gtttcgacct
     2521 caacgggcat cacggaactg catttcgtac a
//
LOCUS       DBNGZD010000228         5572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_659954, whole genome shotgun sequence.
ACCESSION   DBNGZD010000228 DBNGZD010000000
VERSION     DBNGZD010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_659954"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1406)
                     /gene="sucB"
                     /locus_tag="AC16GM_05900"
     CDS             complement(138..1406)
                     /gene="sucB"
                     /locus_tag="AC16GM_05900"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR01347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR2923246.1"
                     /translation="MAATVVIPQMGESVVEATISKWLKKEGDPVKRDEPLVEIMTDKI
                     NAEIPAHKAGVMGKILVPEGTTVPIGSQIAWILDPGEKMPTDGKGADSVQLTPTAAPA
                     KPQAAPAPAAAAVEAEERESIRTSPAVRRLAKEFDVDLAQVRGTGGGGRITKEDVEAY
                     VAARKKGPAAPAAPKAAPPTAPRPIVAGPREEIVPFAGVRKVIADHMIKSKHTAAHYT
                     TFDEADMSRLVELRTKYKEPIKAKYGFNLTYMPFLIKASCMALKEFPVVNASINEDKI
                     IYKKYYNIGVAVGRDSGLIVVVVKDADQKGILEIAREVNELSAKAHADKLAIDDITGS
                     TFTITNAGMFGALGSTPIIHYPEVAILGVHMIQKRPVVRENQIVARDMMTLTLSLDHR
                     LVDGHIGVQFLRKLITYLEDPVMLFTTDWL"
     gene            complement(1518..2501)
                     /locus_tag="AC16GM_05905"
     CDS             complement(1518..2501)
                     /locus_tag="AC16GM_05905"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR2923247.1"
                     /translation="MRQITYIEAITEAMTQEMERDPRVFVMGEDIGLYGGVFKATKGM
                     QKRFGEYRVLDTPIAENLIVGLGVGAAIQGMRPISEIQFSDFFTYSMDQTVQQAAKLR
                     YRSGGTWTVPMVIRVCCGGEVGGGLYHSQTNEAWYLHCPGLKVVVPGTPYDAKGLLLA
                     AIRDPDPVVYFEHKRLYRSLKADVPEGDYTVPIGKASVVRAGKDITIVAYQLMLQKSL
                     EAAQLAAKEGIDVEVIDLRTLLPIDKATILESVKKTGRVLLVQEAPKTGGAAAELSAI
                     ISEEAFDFLDAPIVRLAGLDIPPIPFAPPLEHFYLPNAQKILAKLRELKAY"
     gene            complement(2614..3648)
                     /locus_tag="AC16GM_05910"
     CDS             complement(2614..3648)
                     /locus_tag="AC16GM_05910"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR2923248.1"
                     /translation="MRSVAAPPPPQAAREVKVQAYPGLTDKEKKDLYTLLVRCRTLDE
                     RMRKLFRAGRFAGTYFSGVGQEATTIGTAYNLRQQDVIAPAHREIGASVAKGVPTLQL
                     IAQAYARKNSVDKGKSHPCHYGWAPLNVINPASTIAGQTVVGTGCALAFKLRREDHVV
                     LAYCGEGATANGAWYEALNFAAIHKLGMIFVVQNNLWAESVPARLGAALEDYHHRADG
                     VGCPGVSIDGNDVFRVYEVAREAIARARKGLGPTVIECKTYRWYGHSEIDPADYRTQK
                     ELEYWQSLDPVPRAEKHLRELGILTDALKQKIISDLEKEVEEAVDICENTPYAEPEEA
                     LRDVYADSPK"
     gene            complement(3829..4086)
                     /locus_tag="AC16GM_05915"
     CDS             complement(3829..4086)
                     /locus_tag="AC16GM_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HIR2923249.1"
                     /translation="MRKITREELKRCNGANGSPIYIAYKGKVYDVTLSPLFFDGVHFD
                     HFAGDDLTEVLQDAPHGEEVFDLYPCVGELTDEAPARTGGE"
     gene            4396..>5572
                     /locus_tag="AC16GM_05920"
     CDS             4396..>5572
                     /locus_tag="AC16GM_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2923250.1"
                     /translation="MMQTMRNITKMVMWVVLAGFVGLIVFQWGMQQQSASKMKGILGK
                     VNDVEIQPALYDYYYNRLYEQQKQQKGEVPEEQVPALRQSAWNGLVNDLLMQGEVRKF
                     DLRVTDPELVDFLKKFPPPELQKLPDFQTEGKFDYTKYLQIMANPAAAPLWSQVEDLV
                     RPRLLQIKTEQFILSPARLTDEEVQDYFRTQNEKVKVRYIFIPNREFEPQVASLSDAE
                     VEAFYTKNKERYRMVERGALKYVEWAKTPTAADTTRVTNQLEALRQQALAGADFAELA
                     REYSQDPSAAQNGGDLGWFGKGQMVGPFEQAAYALKPGEISPPIKSPYGYHIIKLHER
                     KTEKKEEKVHASHILLKVEVSSETLERLKREAEDFTAAARKVGFDRAAAEMKLEVKPT
                     "
BASE COUNT         1043 a         1724 c         1564 g         1241 t
ORIGIN      
        1 cgctttttac cattccccat cccgacacga tctgtttcga gtgtcgtcac accgttttct
       61 tggttttttg tggccattct atttaccctc cgtactaaca taatacttct taaacatctt
      121 caacgcaatc ttctttccta tagccaatcc gtcgtaaata gcatcaccgg gtcttcgagg
      181 taggtgatca gtttccgcaa aaactgcacc ccgatgtggc cgtccaccaa tcggtggtcc
      241 agcgagagcg tcaaggtcat catgtcgcgg gcgacgatct ggttctcccg caccaccggc
      301 cgcttctgga tcatgtggac gcccaagatc gccacttccg ggtagtggat gatcggcgtc
      361 gagcccagag cgccgaacat cccggcattg gtgatggtga aggtcgaccc ggtaatgtcg
      421 tctatggcca acttgtcggc gtgcgctttg gctgaaagtt cgttcacctc gcgggcgatc
      481 tccagaatcc ccttttgatc ggcgtctttg accaccacca cgatgagccc actgtcccgc
      541 cccaccgcca cgccgatgtt gtagtacttc ttgtaaatga tcttgtcctc gttgattgag
      601 gcgttgacta cggggaattc tttcaacgcc atgcaggagg ccttgatcaa aaacggcatg
      661 taggtcaggt tgaagccgta tttggctttg atcggctcct tgtacttcgt tcgcaattcg
      721 accagtctcg acatgtccgc ttcgtcgaag gtcgtgtagt gcgccgccgt gtgcttcgat
      781 ttgatcatat ggtcggcaat caccttgcgc accccggcaa agggaacaat ctcttcgcgt
      841 ggcccggcga caatgggacg cggagcggtc ggcggcgccg ctttgggagc ggccggtgct
      901 gccgggcctt tcttgcgcgc cgcgacgtac gcttccacat cctctttggt gatccgcccg
      961 ccgccgcctg tgcccctcac ctgcgcgaga tcgacgtcga actctttcgc cagccggcgc
     1021 accgccggtg acgtgcggat tgactcccgt tcttctgctt caactgcggc agctgccggg
     1081 gccggtgcgg cctgcggctt agcgggcgcg gcggtcgggg tcaactgcac gctgtcagca
     1141 cctttaccgt cggtcggcat cttttcgccc gggtccaaaa tccaggcgat ctgcgaaccg
     1201 atgggcacgg tcgttccctc cggtaccaga atcttgccca tcaccccggc tttgtgcgcc
     1261 ggaatttcgg cgttgatctt gtcggtcatg atttccacca ggggctcatc ccgcttcacc
     1321 gggtcgcctt cctttttgag ccacttggaa attgtcgctt cgacgaccga ctcccccatt
     1381 tgcggtatta ccactgtcgc agccatttac accctccgtc tatccttgaa gattaaggca
     1441 ccattaccga agttttaccc gacacgcgta ggcggcactc ttagtgccgc cctttaattg
     1501 tcccacaatc ctctgcttta gtacgctttc aactctcgta acttcgccag aatcttctgc
     1561 gcattgggca ggtagaaatg ctccaggggc ggcgcaaagg ggatgggcgg gatgtccaat
     1621 ccggccagtc gcacgatcgg cgcatctaag aaatcaaacg cttcttcgga aatgatggcc
     1681 gaaagctccg ccgctgcgcc gccggttttg ggcgcttcct gaactaagag cacccggccg
     1741 gtttttttca ctgattccag aatcgtagcc ttgtcaatcg gcagaagcgt ccgcaggtcg
     1801 atcacctcaa catcaatgcc ttccttggcc gccagctgcg ccgcttctaa ggacttctgc
     1861 agcatcaact ggtaggcgac gatggtgatg tcctttcccg ctcgtacgac gctggccttg
     1921 ccgataggca cggtgtagtc accctcgggc acgtcggctt tgagtgagcg gtagagccgc
     1981 ttgtgttcga agtagaccac cggatcggga tcgcgaatcg cagccaaaag caatcctttt
     2041 gcgtcgtacg gcgtaccggg gacgacgact ttgagcccgg gacagtgcag gtaccaggct
     2101 tcattggttt gggagtgata caacccgccg cccacttcac cgccgcagca gacccggatg
     2161 accatcggca ccgtccaggt cccgccggag cggtagcgca gtttggccgc ctgctggacg
     2221 gtctgatcca tcgagtaggt gaagaagtcg gagaattgga tttcggaaat cggccgcatc
     2281 ccttggatcg ccgcgcccac ccccaggccg acgattaagt tttcggcgat cggcgtatcc
     2341 agcacccggt actcaccgaa gcgcttctgc atccccttgg tggctttgaa caccccgccg
     2401 tagagcccga tgtcttcccc catgacgaag acgcgcgggt cgcgctccat ttcctgagtc
     2461 atcgcctcgg taatcgcttc aatgtaggtt atctgtcgca ttccggtatc cctttgcgtt
     2521 tttcactttc aatcggaaat ttacttttct ccatgtcatc ttgtggtgag tccgcctcag
     2581 gcgaatcgaa ccactgtgcc tcggtggtga gatttatttc ggactgtcgg cgtaaacgtc
     2641 ccgcagcgcc tcttccggct cggcgtaggg cgtgttttcg caaatatcca ccgcttcttc
     2701 cacttccttt tcgaggtcgc tgatgatttt ctgttttagg gcgtcggtca ggattcccaa
     2761 ttcccgcagg tgtttctcgg cccgcggcac cgggtccaga gactgccagt attctaactc
     2821 tttctgcgtc cggtagtcgg ccggatcgat ttcggagtgg ccgtaccagc ggtaggtttt
     2881 gcactcgatc accgtcggcc ccaggccttt gcgcgcccgg gcgatcgctt cccgcgccac
     2941 ttcgtatacc cggaacacgt cgttcccgtc gatgctcacc cccggacaac cgacgccgtc
     3001 cgcccggtgg tggtaatctt ccaacgccgc acccagccgg gccgggaccg attccgccca
     3061 gaggttattc tgcacgacga agatcatccc caatttgtga atggcggcaa aattgagcgc
     3121 ttcgtaccag gcgccgttgg ccgtggctcc ttcgccgcaa taggccagca ccacgtgatc
     3181 ctcccggcgc agtttgaagg ctaaagcgca gccggtgccc accacggtct gcccagcgat
     3241 ggttgaggcg ggattgatga cgttgagcgg cgcccagccg tagtggcagg ggtgggattt
     3301 gcctttgtcg acggagtttt tccgggcgta ggcctgggcg atgagttgca gggtcggcac
     3361 tcccttggcc accgaggcgc cgatctcccg gtgcgccggg gcgattacat cttgctgccg
     3421 gaggttgtag gcggtgccga tggtggtcgc ctcctgtccc acgccggaga aataggtccc
     3481 cgcgaagcgc ccggcccgga acaacttccg catccgttcg tctaaggtcc ggcagcggac
     3541 gagaagggtg tagagatctt ttttctcttt gtccgttaat cccgggtacg cttgaacttt
     3601 tacctcccgc gccgcctgcg gcgggggagg cgcggcaact gagcgcattg tttttctgcc
     3661 tccgtgaaaa attacctttt ttgtcccgct tctggcgggt ttttgactcc ttcgcgtacg
     3721 tcgatccgcc actggtcaaa aactcctgcg ggtcggaaac tgtacaatta atacgaaatt
     3781 cggcggttgg caagtggtgc gcagacaggc aggagatgcg gcacggttct actctcctcc
     3841 ggtgcgagcc ggcgcctcgt cggtcaactc ccccacgcag gggtagaggt caaacacttc
     3901 ctcgccgtgc ggcgcatcct gtagcacttc ggtcaggtca tccccggcga agtggtcaaa
     3961 atgcacgccg tcgaagaaca aaggactcaa ggtgacgtcg tagactttcc ccttgtaggc
     4021 gatatagatc gggctgccgt tggccccgtt gcaccgtttc agttcttccc gggtaatttt
     4081 cctcaagttc cgtcgccatc ctccgcccgt cgtgccagaa tgtagacccc ggccaccgaa
     4141 tgtcaaccgt acggggccgc gctgcccgaa ccggggacag ggttcgctcc cgtcagaaaa
     4201 cttacccccc caacttctcc acttgtttgt ggctttctct tccgtatatt cccctcagtc
     4261 ggtagactcg tagccgaggt ttataacctc ggcggctctc aatgagccgt cgccgttctt
     4321 gggtcggtag tcgggctcgt ctcgagcccg actggttatt ggtcttggaa aagcatcgat
     4381 gaggagtaac accgtatgat gcaaacgatg cgcaacatca ccaagatggt gatgtgggtg
     4441 gtccttgcgg gcttcgtcgg cttgattgtc ttccagtggg gcatgcagca gcagtccgcc
     4501 tccaagatga agggcatcct gggcaaggtc aacgacgtcg aaatccagcc ggcgctctac
     4561 gactactact acaatcggct gtacgaacag cagaagcagc aaaaaggaga ggtccccgaa
     4621 gagcaggtgc cggccctgcg tcagtcggcc tggaacggct tggtcaatga ccttttgatg
     4681 cagggcgagg tgaggaaatt cgacctccgg gtgaccgacc ccgaattagt cgatttcctg
     4741 aaaaaattcc ccccgccgga actgcagaaa cttcccgact tccagaccga gggcaagttc
     4801 gactacacca agtacctgca gatcatggcc aaccccgccg ccgcgcccct ctggagccag
     4861 gtggaagatc tggtccggcc gcgcctcttg cagatcaaaa ccgaacagtt catcctctcc
     4921 ccggcccgcc tcaccgacga agaagtgcag gactacttcc gcacccagaa cgaaaaagtg
     4981 aaggtgcgct acatcttcat ccccaaccgg gagttcgaac cccaggtggc gagtctgtcc
     5041 gatgccgagg tggaggcctt ctacacgaag aacaaggagc gctaccggat ggtcgaacgc
     5101 ggggcgctca agtatgtgga gtgggccaag acgccgaccg ccgccgacac gacccgggtg
     5161 acaaaccagc tggaggcctt gcgccagcag gccttagccg gggccgactt cgccgaactc
     5221 gcccgtgagt attcccagga tccctcggcc gctcagaacg gcggtgatct gggctggttc
     5281 ggcaaggggc agatggtggg gccgttcgaa caggccgcct acgctctcaa accgggggaa
     5341 atttcgccgc ccatcaagtc gccctacggt taccatatta tcaaactgca cgagcggaag
     5401 actgaaaaga aagaggaaaa agtccacgcc agccacatcc tgttgaaagt cgaggtctcc
     5461 tccgaaacgc tggagcgtct caagcgcgag gcggaagatt ttactgccgc cgcccgcaaa
     5521 gtgggcttcg accgcgccgc cgccgaaatg aagttggaag tcaaaccgac cc
//
LOCUS       DBNGZD010000229         5058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1286539, whole genome shotgun sequence.
ACCESSION   DBNGZD010000229 DBNGZD010000000
VERSION     DBNGZD010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1286539"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..925
                     /locus_tag="AC16GM_05925"
     CDS             152..925
                     /locus_tag="AC16GM_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923251.1"
                     /translation="MVKLKMLYRLGLCLLALSLGCGSNDNAVKPRLCMFVGVDISGSF
                     MRERGEYFDDSMDFLAHYLYSHLNGLGGLEVPSVLFVGSIGGAKPDEPKTFFPIQSFQ
                     GKTVEEIRNKLKEIFPKEDPNPFTDYNAFFEQVALTAKNKNLVLRPISIVMISDGIPD
                     VKKQGKTDFRSIVVHPLEKLARNITIRLLYTDAVVGKNWQTKVKRSRVKIWTQDAEVM
                     AFWKDPKILTPEKPLAEQTKFFGWVKDNVDFGVRARRID"
     gene            complement(1020..1610)
                     /locus_tag="AC16GM_05930"
     CDS             complement(1020..1610)
                     /locus_tag="AC16GM_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923252.1"
                     /translation="MITENALSYILFKNYCESDSRVFFKFLHNISVKRKRDWSYWCAP
                     EVDVIEVKKDETIIGYELKADQRRKGREEPPAFFEGVDQAIAYLDLPRICENGQEKFK
                     GGAFEFVYLVYTRRNGDFSHREIRIFNLLSIGVIMVLPNGECKKVKEASVNPLLDLDA
                     KSHFLNNLNTLEKHTTSNKIFSKIRAQGEAYLSGLR"
     gene            complement(1630..1788)
                     /locus_tag="AC16GM_05935"
     CDS             complement(1630..1788)
                     /locus_tag="AC16GM_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2923253.1"
                     /translation="MSENRDYWFVYLLRCADGSIYTGITNDVEARVREHNQDIVGLTP
                     AMPWFILL"
     gene            complement(1911..1997)
                     /locus_tag="AC16GM_05940"
     tRNA            complement(1911..1997)
                     /locus_tag="AC16GM_05940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1961..1963),aa:Ser,seq:gga)
     gene            2209..2856
                     /locus_tag="AC16GM_05945"
     CDS             2209..2856
                     /locus_tag="AC16GM_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR2923254.1"
                     /translation="MRNNKRISESTIHRLSLYYRSLNALESEGYATISSRELAKRERL
                     TPAQVRKDLSFFGTFGTRGLGYPVHELKEKISAIMGLNRTWNVAVVGAGNIGSALVAY
                     REFSRQGFSIRAIFDNDRRKIGSTHEGVTVSDSKNLTPELKEKNIDIVILAVPAPVAQ
                     VVTDDIVAAGVRAILNFAPVRLKVPDDVILRNENMSIELEHLSFALMNRNSPPRG"
     gene            2925..4022
                     /locus_tag="AC16GM_05950"
     CDS             2925..4022
                     /locus_tag="AC16GM_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923255.1"
                     /translation="MAGDDTPHHLHYDYRDIFIAPRLALSGKKILGHFIGLVIGYGGY
                     VFFTYLALISLGESFDAVWANFGLAPYLPLEDVPWFGALLAVLGLLWLLLAWLVSSFT
                     VAKVTFEELRGNLFYSLREGWAFARTNLKKLFAPFFFSVLFVVALLLLLVFAGLFSRI
                     PWLGELSFSLFFLVPLFAFAAVVVVTLVGLAMGVLLLPAAVVAEEKGSFEATAHLFSL
                     LWRQPWRWSLYTGLSGLLAKVATFVFGYACFRAAGLVADLASLLGGDKIDFLIDKALE
                     YLPAEANLVLFFSTLFPGLDFGFHLPIAEGTPDLPVVQEIAGLVLGLGFVTLFLAVVA
                     YGLAILSSGQLLTYLSLRHKQGVDLLQPRET"
     gene            4128..4514
                     /locus_tag="AC16GM_05955"
     CDS             4128..4514
                     /locus_tag="AC16GM_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR2923256.1"
                     /translation="MGKQALATTGAPKAIGPYSQGIKAKCQQLLFVSGQIASKPENGE
                     LVAGGIIEQTKQALENVKAIVAAGGGTLADVVKTTVYLTNFDDFTLMNETYSRFFAEM
                     PPARATVQVAKLPKGAKIEIDVVAAL"
     gene            4566..>5058
                     /locus_tag="AC16GM_05960"
     CDS             4566..>5058
                     /locus_tag="AC16GM_05960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2923257.1"
                     /translation="MIDFQLTEDHLAIQQLVRDFAAKEMAPGLRERDRQEKFDPAILK
                     KLGAADILGLCIPAKYGGAGFDYISLGLACEELEYVDTAGRVILSVHIGLNSLALLTW
                     GTEAQKQKYLAPQAKGDKIATFGLTEPNAGSDAVGIQTTADREGDAFVLNGEKMWISL
                     ADVA"
BASE COUNT         1137 a         1389 c         1345 g         1187 t
ORIGIN      
        1 ccaaagcgct gctggacaat ttctcccgaa ggggccgaaa gaaagctccg gaaagagtgc
       61 cagaaagaat tccagaaaaa gctccagaaa gagtcgcaga agaagagtta gtcccttgag
      121 acagtcgaaa acattttggg agaagaaccg aatggtaaaa ctcaaaatgc tttaccggct
      181 tgggttgtgc ctgctggcat tgtctctggg atgcggttcg aatgacaatg ccgtaaaacc
      241 gcggctgtgc atgttcgtgg gagtcgatat cagcggttca tttatgcgag agcggggtga
      301 atattttgac gactcgatgg actttctggc tcattattta tacagtcacc taaacggctt
      361 gggcgggttg gaagttccga gcgttttatt tgtcggttcc atcggtggcg ccaagcccga
      421 cgagcccaag acctttttcc ccatccagtc cttccagggc aaaacggtgg aggaaatcag
      481 gaacaaactg aaggaaatat ttcccaagga agaccccaat ccctttaccg actacaacgc
      541 ctttttcgag caggtggccc tgaccgccaa aaacaaaaat ctggttctcc ggcccatctc
      601 gattgtcatg ataagcgacg gcataccgga cgtgaaaaaa caggggaaaa ccgattttcg
      661 gagcattgtt gttcatcccc tggagaaatt ggcccgcaat atcaccatcc gcctgctgta
      721 tacggatgcg gtggtgggga aaaattggca gaccaaagtg aagcggagcc gggttaaaat
      781 ttggacccag gatgcggagg tcatggcctt ctggaaagat cccaaaattt tgaccccgga
      841 aaaacccctg gcggaacaga cgaaattttt tggctgggta aaagataacg tggatttcgg
      901 cgtgcgcgcg cggcgaatcg actgattttc ttatttaccc agcccgacct acatctggtt
      961 cataatgaag taatcaagtg aggcaaaaca ccccccggca gaggccaggg gctgttcaac
     1021 tatcttaaac ctgaaagata ggcttcacct tgcgctctga tttttgaaaa aatcttgttg
     1081 ctagttgtat gtttttctaa agtattcaaa ttgtttaaaa aatgactttt ggcatctaag
     1141 tccaaaagag ggtttaccga ggcttccttg acttttttgc actctccatt tggtaaaacc
     1201 ataataacac cgatagacaa caaattaaaa atacgaatct cacgatgtga aaaatcgcca
     1261 tttcgcctcg tataaactaa gtatacaaat tcaaaagctc cccccttgaa cttttcttgt
     1321 ccattttcgc agattcttgg caaatcaaga tatgcgatcg cttgatcaac gccctcgaaa
     1381 aacgctggag gttcttctcg accttttctg cgctgatctg ctttcaattc atagccaatg
     1441 atagtttcat ctttttttac ttcgattaca tcgacttcgg gagcgcacca ataagaccaa
     1501 tcccttttgc gttttacact aatattgtga aggaatttaa aaaatactct gctatccgat
     1561 tcgcaatagt ttttgaataa aatataagag agagcatttt ctgtaatcaa gataatcgac
     1621 tcacctcctc tacaacaaaa taaaccacgg cattgctggg gtcaacccga caatgtcctg
     1681 gttatgctcc cgcactctcg cctctacatc gttggtgatc cccgtataaa tagaaccatc
     1741 ggcgcaccgg agcaagtaga cgaaccaata gtctctgttc tcggacatcg atcaatccct
     1801 cgattcgtct cccgcctgtg gcgggacgag actcactcgg gataaaccct tcgacttcgc
     1861 tctcgttcag agcctgaagg ttcttcagag ccgatggcag ggctttacag cggagagact
     1921 gggattcgaa cccaggaggg ccttacgacc ctacatgctc tccaggcatg ctccttcagc
     1981 cactcggaca tctctccgga cgactgtctc aatttaagcc gagtggttgg ctctaacaag
     2041 acgtgcacgg ctctctgcct cgagcgatcc cgcctctcct aaaacatgca aattgggccg
     2101 gtgattgtga cctcgaaacc gccccaaagg caagcccgcc actgaaattt tctattgacg
     2161 aaagaccttc ctcagttata ttgtgaaaga gttcacaaga ggtatggcgt gcggaataac
     2221 aagcgcatat ccgaatcgac cattcaccgg ctctccctct actaccgctc actcaacgca
     2281 ctggaaagcg aggggtacgc caccatttca tcgcgcgaat tggccaagcg ggagcgtttg
     2341 accccggcgc aggtgcggaa agatctttcg tttttcggga cgttcggcac ccgcggtctg
     2401 ggatatcccg tgcacgagtt gaaggaaaag atctccgcca taatggggtt gaaccgcacc
     2461 tggaacgtgg cggtggtggg cgcggggaac atcggctcgg ctctggtcgc ctaccgggag
     2521 ttcagccggc agggattcag catccgggcg atcttcgaca acgaccggcg caagatcggt
     2581 tccacccacg aaggggtgac cgtctccgac agcaaaaatc tgaccccgga attaaaagag
     2641 aaaaacattg acatcgtgat cctggcggtg ccggcgccgg tggcgcaggt tgtgaccgac
     2701 gacatcgtgg ccgccggggt gagggccatc ttaaactttg cgccggttcg gctcaaggtg
     2761 cccgacgacg tcatcctccg gaacgagaac atgtcgatcg aactggagca cctttccttc
     2821 gccctgatga accgcaactc gccgccgcgc gggtgagccc tcttttcggc ttgacagatt
     2881 cccccccgac ctatactgtc tccgggaggg acggaggcag accgatggcc ggggatgaca
     2941 cgccgcacca cctgcactac gattaccgcg acatcttcat tgcgccgcgt ttagcccttt
     3001 cgggcaagaa aatcttaggg cacttcatcg gcttggtgat tggctacggc gggtacgtct
     3061 ttttcactta cctggcgctg atttctctgg gcgagtcgtt cgatgccgtc tgggccaact
     3121 tcggtctggc tccctatctc ccgctggaag acgtaccctg gtttggggcg ctcctggccg
     3181 tcctgggcct cctctggctc cttttggcct ggctcgtgtc gagttttacc gtggcgaagg
     3241 tcaccttcga agagttgcgg ggcaacctgt tctattccct gcgcgaaggc tgggcctttg
     3301 cccggacaaa tctcaaaaaa ctcttcgcgc cgttcttctt ctcggtgctc tttgtagtcg
     3361 ccctcctgct gcttttggtt ttcgccggcc ttttcagccg tattccctgg ctgggagaac
     3421 tctccttttc gttattcttc ctggttccgc ttttcgcctt tgcggcggtg gtcgtggtga
     3481 ccttggtcgg gttagctatg ggagtgctcc tgctccccgc cgcagtggtg gccgaagaga
     3541 aaggttcgtt cgaagcaacg gcccacctct tttcgcttct ctggcggcag ccctggcgct
     3601 ggagtctcta caccggactt tccggactct tggcgaaggt cgccacgttt gttttcggtt
     3661 acgcttgctt ccgcgccgcc ggcctggtgg ccgacctggc gtcgcttctc ggcggggaca
     3721 agatcgattt tctgatcgac aaagccctgg agtacctgcc cgccgaggcc aatctggtct
     3781 tatttttttc gactttgttt cccggactgg acttcgggtt tcacctcccg attgccgaag
     3841 gcacgccgga cctgcccgtg gtgcaggaga ttgccggact ggttttgggc ttgggcttcg
     3901 tgacgttgtt tttggctgtc gtcgcctacg gtctggcgat cctctcctcc ggacaactgc
     3961 ttacctacct cagcctgcgg cacaagcagg gagtggacct gctccaaccc agagagacct
     4021 aagccagagc gctcttttcg gttgaccact ttctgaggcc gaactatctt ctgtccgatt
     4081 tttcgtcgca tgcaaccggt gatacgaacc gcagcggaga aaccgaaatg gggaaacaag
     4141 cccttgccac gaccggagca cccaaagcca tcggccccta cagccagggg atcaaagcga
     4201 agtgtcagca gctgcttttc gtctccggtc aaatcgcctc aaaaccggag aacggtgaac
     4261 tggtggcggg tggcatcatt gagcagacca agcaggcact ggagaatgtc aaagcgatcg
     4321 tcgcagccgg cgggggcacg ctggcagacg tggtgaagac cacggtctac ctgacgaact
     4381 tcgacgattt caccctgatg aatgaaacct acagccggtt ttttgccgaa atgccgccgg
     4441 cccgggccac cgtgcaggtg gccaagctcc ccaaaggcgc gaaaattgaa atcgacgtcg
     4501 tcgccgcgct gtaaatttcc agcggagccg gaacgggaaa cgatttctcc tgtattgagg
     4561 gagatatgat cgactttcaa cttaccgaag accacctggc cattcaacaa ttagtccgcg
     4621 atttcgctgc gaaggaaatg gcgcccggcc tgcgggagcg cgaccggcag gagaagttcg
     4681 acccggccat cctcaagaaa ttgggtgcgg ccgacatcct ggggctctgt atcccggcca
     4741 agtacggcgg ggcgggattt gactacatca gtctcggcct ggcctgcgag gaactggagt
     4801 acgtcgacac cgccgggcgg gtgatcctct cggtgcatat tggactcaat agtcttgcgc
     4861 ttttgacctg gggcaccgaa gcgcagaagc agaaatacct cgccccgcag gcgaaaggtg
     4921 ataagattgc aaccttcggc ttgaccgaac cgaacgccgg gtcggatgcg gtggggattc
     4981 aaaccaccgc cgaccgggag ggcgatgctt tcgttctgaa cggcgagaag atgtggattt
     5041 ctttagccga cgtggccg
//
LOCUS       DBNGZD010000230         2267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_592882, whole genome shotgun sequence.
ACCESSION   DBNGZD010000230 DBNGZD010000000
VERSION     DBNGZD010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_592882"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="AC16GM_05965"
     CDS             complement(<1..263)
                     /locus_tag="AC16GM_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="High-affinity nickel transporter"
                     /protein_id="HIR2923258.1"
                     /translation="MISVVVGFLAGSLHVLSGPDHLAAVAPLAVSGQRRVWLTGFRWG
                     VGHACGVGLVGIFALLLRGILPIDWLSSWSERLVGLVLVGIGI"
     gene            complement(342..482)
                     /locus_tag="AC16GM_05970"
     CDS             complement(342..482)
                     /locus_tag="AC16GM_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923259.1"
                     /translation="MTPGIKYAPTDAVGLAGGVQIPIHQDLFGIQDEDPVFIVGLAYD
                     WR"
     gene            complement(778..>2267)
                     /locus_tag="AC16GM_05975"
     CDS             complement(778..>2267)
                     /locus_tag="AC16GM_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2923260.1"
                     /translation="FFLGELTGTLTYWRNSGTPLSPNFVFGANDFDSIVAFPTVGCSV
                     SVAPPPPGQLAEAQAHGINGIQFADLDGDGDFDFFWGDIFNTNMYFFRNQGTPLNSHL
                     QAITCTFLPVSTIGFNEPAFADLDADGDPDLVVGSANFGSSIDNLLYFRNVGSPTAPR
                     ESLITRNLLSNLDFGANSYPAVVDWDGDGLPDLFCGRDDGRMAAYRNVGSQRHPAWQF
                     VSSGFGGVDVGASSTPALGDLDGDGDLDMICGNEDGRLALFRNTGTPSNPVFTLTDGF
                     FLGLDLDFNASPVLVDIDHDGDLDLFVGEWDNQANANLYFYRNEGGPFPPNFVQEATA
                     LLPPTGLQQTLPAFADIDGDGDYDLFLGTSDGTIWFYRNSGDSTTFNFVKETENFENI
                     DVGQTAAPFLVDIDYDGDPDLVAGESDGGLNLYCNQALAVGDVTRDGVVDISDLVSLI
                     QRVVFGALLATPDATGDLNCDGVLDIIDIVALVQNVVFGAPPPCI"
BASE COUNT          562 a          685 c          620 g          400 t
ORIGIN      
        1 cagattccta tgccaaccag gaccagacca accaatcgtt cactccagga agaaagccaa
       61 tcaatcggta aaattccccg cagcagaagg gcgaaaatac ccactaggcc aaccccgcag
      121 gcatggccta cgccccagcg aaagccggta agccacaccc gccgctgccc ggaaacggcg
      181 aggggtgcca ccgcagccag atgatcaggg ccggaaagga catggagcga accggccaga
      241 aagccaacga caacagaaat catcttttta atctttcaaa aaggacactt tcatagggcc
      301 aaggttactg ccgacagttg ggcgggcaaa cttctgaaat cttaccgcca gtcgtacgcc
      361 agtcccacga tgaaaaccgg gtcttcgtcc tgaataccga agagatcctg gtgaatcgga
      421 atctgcaccc caccggccag tcccacggcg tccgtgggcg catatttgat ccccggcgtc
      481 acgtaggtga cccacccacc cgtatgaggc tcaaccagac cggatatttc gtccttatct
      541 gcaaagcgcc cgtttatctc cgcagccccc ggccaggcag accgaacagg cctgcgcctg
      601 ctgatagaat accacccaaa agccgcagag gacactgaaa acggaaaacg tactaaccac
      661 tttcctcacc tgcgtaactt gctccttcca acgaccagga aacgccgtac ggtcacttca
      721 cgaaatatac taaccttccc cgaatcacgt tagtttctca caaggttcac ggattgctta
      781 gatgcacggc ggcggggcac cgaacaccac gttctgaacc agggcgacaa tatcgataat
      841 atccaaaacg ccatcgcagt tcaaatctcc ggtggcgtcc ggcgtagcca agagggcgcc
      901 gaaaaccacc cgttgaatca aactgacgag gtcactgatg tcgaccaccc cgtcgcgggt
      961 cacgtcgccg acggcgaggg cctgattgca gtagaggttc agcccgccgt cgctttcccc
     1021 agcaaccaga tccggatcgc cgtcgtaatc aatgtcaacc agaaagggag cggcggtctg
     1081 gcccacatca atattctcaa agttctcagt ttctttgacg aaattgaagg tggttgaatc
     1141 cccgctattg cggtagaacc aaatcgttcc gtcgcttgta ccgagaaaga gatcgtagtc
     1201 cccgtcgccg tcaatgtccg caaaagccgg cagggtctgc tgcagaccag tgggggggag
     1261 taatgccgtc gcttcctgta cgaaattcgg cggaaagggt cccccctcgt tgcgatagaa
     1321 atagagattg gcattggcct gattatccca ttccccgacg aacagatcta aatcgccatc
     1381 gtggtctatg tccaccagaa ccggagaagc attgaaatcg agatccaacc cgaggaagaa
     1441 accgtcggtc agggtaaaaa cgggattgct cggcgtgccg gtattgcgaa agagcgccag
     1501 ccggccatct tcgttgccgc agatcatatc gagatcgcca tccccgtcca gatcgcccaa
     1561 agccggagtg ctggaggcac ccacatctac gccgccgaag ccgctggaaa cgaattgcca
     1621 ggcagggtga cgctggctgc cgacattccg gtaggcggcc atccggccgt cgtcccggcc
     1681 gcagaagagg tccggcaggc cgtctccgtc ccagtccacc accgctggat aggagttggc
     1741 gccaaagtcc agattcgaga gcaggttcct ggtaatcaag gattcccgcg gcgctgtcgg
     1801 gctaccgacg ttacggaaat acaagaggtt atcgatgctc gaaccgaagt tggccgatcc
     1861 cacgaccaga tccggatccc cgtcggcgtc caggtcggca aaagccggct cgttgaaacc
     1921 gatcgtgctc accggcaaaa aggtgcaggt gatcgcctgg aggtgggagt tcagcggggt
     1981 gccctgattc cggaaaaaat acatgttcgt gttgaaaatg tccccccaga agaaatcgaa
     2041 gtcgccgtcc ccgtccagat cggcgaactg aatgccgttg atgccgtggg cctgtgcttc
     2101 agccagttgg ccgggaggcg gcggtgccac ggaaaccgag cacccgacgg tcggaaaggc
     2161 gacgatggaa tcgaaatcgt tcgcgccgaa gacgaaattg ggcgaaagcg gggtgccgct
     2221 gttccgccag tacgtcaggg tgcccgtcaa ttcccccaga aagaagt
//
LOCUS       DBNGZD010000231         3308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1217167, whole genome shotgun sequence.
ACCESSION   DBNGZD010000231 DBNGZD010000000
VERSION     DBNGZD010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1217167"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..454
                     /locus_tag="AC16GM_05980"
     CDS             287..454
                     /locus_tag="AC16GM_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923261.1"
                     /translation="MQNKKGKNYREPLPKSLSSLARCQNKSTQKGKNTPCPLPFEGES
                     SEGKRIIPLRS"
     gene            complement(499..768)
                     /locus_tag="AC16GM_05985"
                     /pseudo
     CDS             complement(499..768)
                     /locus_tag="AC16GM_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
     gene            1036..3195
                     /locus_tag="AC16GM_05990"
     CDS             1036..3195
                     /locus_tag="AC16GM_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HIR2923262.1"
                     /translation="MKRLSLTVALLVAGLAPAQARIIVAPPPGQLNFPPYLSIKNYDV
                     RVKITGPMAVTTIEQVYQNDCAQMLEGTYLFPIPEGAALSKFYLYVNGEEVPGQVLAA
                     GEARQIYEEIVRRLRDPALLEFAGCGLFKARIFPIPAHGDAKIKLSYSQVLKPFNGLF
                     EYKYSFGREALVQTGINNLTFDLQLASSVPLKNVYSPSHEVKIDRSDEKSARVNWSEN
                     SREIGGDFVLYFAVSPKDIAFSILTDRDAGENYFMALLSPEVKPSATKTIFKDIVFIL
                     DISGSMRGEKIDQAKAAVLFCLQSLNAADRFNLVAFNDQVRVFKEGMAAATPVNIREA
                     EVWVAQIEAEGGTNIAGALEAGLKQLAVGRLNYAVFLTDGLPTVGTTDVNEILALAKR
                     ANAGRVKIFDFGVGYDVNTHLLDKLAEQNSGLSDYVIPSENIEVKVSSFYKKISAPLL
                     ANLKLNFGAVKISDVYPNELPDLFAGSQLVVVGKYSGPEQTTVTLTGELNGEKVVYEY
                     PADFSAKTSWEKFIPKIWATRRVGYLIDQIRLSGESREVVDEIVTLSKKYGIINQYTS
                     FLVKVDPAAPLAQTSLQFMQEEAAPLRAEAGREAVAASKVARESKDMATISGSFDALH
                     RRGEDGAVNLATAGGKTFFRQNEKWVESDRDDKKPVLKIQRFSEAYFALLRIRPEFGK
                     YLALGENVSFDAGKVTVEIAADGKEKLTETELNQYFK"
BASE COUNT          821 a          895 c          886 g          706 t
ORIGIN      
        1 tccgctggcg agccatacaa cccaaggttt tgcaacttgt gtgccacgcg atttccagcg
       61 gccattgagc cagacttatt tgtggctcct gagaggagaa gattgggttc gggaataact
      121 atatgactga tattatatta gaaggaacta tttgtgcccc gggaatcgaa gagaagacgc
      181 aatgggagaa gaaccgacac acaacaaaac cggaaacgga aaaaggttcc acccgttatt
      241 ggtagaattt tcctaaggga gaaattttag aaaagtctga gtagtgatgc agaacaaaaa
      301 aggaaaaaac tatagagagc cactgccaaa aagtttgagc agtttggcgc gctgccaaaa
      361 caaatcgacc caaaaaggta aaaacacccc ctgcccgctt ccctttgagg gggagagttc
      421 tgaaggaaaa aggattattc cccttcgctc ttaacgagca aaggctctat gcaaatgacg
      481 gtaaaactaa aaccccgcct atttcaggtg cttggaaatc agcttggtca tttcgaacat
      541 gttgacctgc cgcttgccgc caaacaccgc cttgagcatc tcgtcggcgt taatcatccg
      601 ccggttcttc ttgtcctgga ggcccttccg cttgatgtag gcccacacct tcttggtgac
      661 ttcggtgcgg ggaatggctt tttcacccac gacggcgctt aaggccgcgc tgggcttcag
      721 gggcttcatg aacgctgcac tcggtttccg cttcgttgcc atcacctttt cctcctgtga
      781 aacaggttac ggttcgaccg ctctttcttc ccgccggccg ttctgagccg gcagcatcag
      841 tgggaaaaat agcacccccc tctggggatg tcaactgctt tcagagggaa aaaggcaaaa
      901 aaacactcat tcccccccgt gaccgccggg ctatcctctt ggctcgaccg gcgccccttg
      961 cgaatgaaaa aagcaaaaaa gtcagggaat aaaatcttct cccgtgggtc tgaaaaggag
     1021 agcaaaggag gaatcatgaa acgcctgtcg ttaaccgtcg cgcttttggt cgctggattg
     1081 gctcccgccc aagcccgcat cattgtcgcc ccgccgccgg gacagctgaa cttcccgccc
     1141 tacctgtcca tcaaaaacta cgacgtaagg gtgaaaatca ccgggccaat ggccgtcacc
     1201 accatcgagc aggtgtacca gaatgactgt gcgcaaatgc tggaaggaac ctatcttttt
     1261 ccgattccgg agggggccgc cctgtccaaa ttttacctgt acgtcaacgg cgaagaagtg
     1321 ccgggccaag ttctggccgc cggcgaagca cgccagattt acgaagaaat cgtgcggagg
     1381 ttaagagatc cggccttgct ggaattcgcc ggctgcggac tcttcaaggc gcgcatcttt
     1441 cccatcccgg cgcacggcga tgccaaaatc aaactctcct acagccaggt gctgaaaccg
     1501 ttcaacggct tatttgagta taagtacagt tttggccggg aagcgctggt gcagaccggg
     1561 atcaacaacc tgaccttcga tctgcagttg gcctccagcg tgccgttgaa aaacgtctac
     1621 tcgccctccc acgaagtcaa aattgaccgg tcggatgaaa aatcggcccg ggtgaactgg
     1681 agcgagaact cccgggaaat tggcggtgac ttcgttctct attttgcggt aagcccgaaa
     1741 gacattgcct tttccatcct caccgaccgg gacgccggtg aaaactactt catggccctg
     1801 ctttcgccgg aggtaaaacc ctccgcgacc aagacgattt tcaaagacat cgtcttcatt
     1861 ctggacatct ccggcagtat gcggggcgag aaaatcgatc aggccaaagc cgccgtgctc
     1921 ttctgcctgc agagtctcaa cgccgccgat cggttcaacc tcgtcgcttt caacgatcag
     1981 gttcgggttt ttaaggaagg gatggccgca gccaccccgg tgaatatcag agaagccgag
     2041 gtctgggtag cccagattga agccgagggc ggcaccaata tcgccggcgc tttggaggcg
     2101 gggttaaagc agttggccgt aggtcggtta aattacgcgg tgtttctgac cgatggtctg
     2161 cccacggtcg gcacaaccga cgtgaatgaa attttggcgc tggccaaacg ggccaatgcc
     2221 gggcgggtta aaatttttga cttcggcgtc gggtatgacg tgaacaccca tctgctggac
     2281 aaactggcgg agcagaactc cggcctttcc gattacgtca tcccctccga aaatatcgaa
     2341 gtcaaagtct ccagcttcta caagaaaatt tccgctcccc ttctagcgaa cttgaaattg
     2401 aatttcggtg cggtcaaaat ctcagacgtc tacccgaacg aattgcccga cctttttgcc
     2461 ggttcgcaac tggtggtggt cgggaaatac tccgggccgg agcaaacgac cgtcactttg
     2521 accggagaat taaacggcga aaaagtagtc tacgagtacc ctgccgactt ttcggccaaa
     2581 accagctggg aaaaattcat tcccaaaatc tgggccaccc gccgggtggg atatttgatt
     2641 gatcagattc gtctttccgg agaatcgcgg gaagtggtgg atgaaatagt gacactatcc
     2701 aaaaaatatg gcatcatcaa ccagtacacc tcgtttttgg tcaaggtcga tccggctgcg
     2761 cctttggcgc aaaccagcct ccaatttatg caggaggaag ccgcaccact gcgtgccgaa
     2821 gcggggcggg aggcggtggc cgccagtaaa gttgcgagag agagtaaaga catggccacc
     2881 atcagcggca gctttgatgc cctccacagg aggggtgaag acggagccgt caaccttgcc
     2941 accgccggtg gcaaaacctt cttccggcaa aacgagaaat gggtggagtc cgaccgggac
     3001 gacaagaagc cggtcctcaa aatccagcgc ttttcggagg cctactttgc gctgttgaga
     3061 atccggccgg agttcggcaa atatctggcc ctgggcgaaa acgtcagttt tgacgccggc
     3121 aaagtgacgg tggaaatcgc cgcggacggc aaagagaaac tcactgagac cgaattgaac
     3181 cagtatttca agtagcagta ggagctactg gaaacgaaag ccggcccgca gttagggccg
     3241 gtttttctat ggatatatga ctgattccct tcgctcgccc cgctatcgtc ccggctcggc
     3301 tggaaggt
//
LOCUS       DBNGZD010000232         5793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_175649, whole genome shotgun sequence.
ACCESSION   DBNGZD010000232 DBNGZD010000000
VERSION     DBNGZD010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_175649"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="AC16GM_05995"
     CDS             <1..520
                     /locus_tag="AC16GM_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR2923263.1"
                     /translation="GDAPERAAPPSVSSDAIVSGRSGLWLGVQVADCLPIFLVDPAKR
                     VVTLIHAGWRSTFLGVIPKTLLTLGDSFASTPDRLVAVLGPTLQSCCAEVGPEVALLF
                     PTKNRVARNSAWVVDFPGMVREQLLAGGLSSGKIFVLPVCTRCGGELFCSYRREGREA
                     GRMFAYAGFVQK"
     gene            608..1222
                     /locus_tag="AC16GM_06000"
     CDS             608..1222
                     /locus_tag="AC16GM_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923264.1"
                     /translation="MLKKKMVLWGSLITAGVILSASLQAVAGMGIGVRGGWVNGYKNP
                     AVAGQLAPAQTSLPKQMQMVGLHVKLGTVPVLDLEGSLEYAWKEKKFTYLSNNYNFRL
                     SDISANATAEYVLPFPVLKPHIGAGLGLHRIVYSVKDPQAPYPFPLPGDGTKVGYHAL
                     VGVTFHPPLFPLEFFGDWRYTWVTTTGKQTRYSTLLAGVTLGLP"
     gene            1544..2161
                     /locus_tag="AC16GM_06005"
     CDS             1544..2161
                     /locus_tag="AC16GM_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923265.1"
                     /translation="MLRTMRFGVVAFVACIGMARAQKDQFGVVDTVSVAVEKGKGEGN
                     WVATVALANDEKLQGLTVPLKYPEGCKVDSVSFADTRLVSFWMKVGNTNTSVQNAVLL
                     FGIATADQKKQYLEPGRGPIARIYFSAPRELAMTAASLDTTRLPLHNVLQLVTPDSKS
                     IYPAFVNGAAVSAPAVVPAAASTDTAAAKKAPAKTATPTKSKKKK"
     gene            2275..2351
                     /locus_tag="AC16GM_06010"
     tRNA            2275..2351
                     /locus_tag="AC16GM_06010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2309..2311,aa:Val,seq:gac)
     gene            2543..4510
                     /gene="thrS"
                     /locus_tag="AC16GM_06015"
     CDS             2543..4510
                     /gene="thrS"
                     /locus_tag="AC16GM_06015"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR2923266.1"
                     /translation="MTVGRNSPQQYPRGITPAEILQRIRHPKPEAILAAKVNGRVVDL
                     NRPLAADSTVEFLTFDEAEGREVFWHSSAHVMAQAVTELFPGTKLAIGPPIEEGFYYD
                     FDRGEPFSPEDLEQIEKRMRKIVAGAYPFQRTECSREEALERFGPLGEKYKLEILEAI
                     PPDEQVSLYTHATFTDLCRGPHLPDTGGIKAVKLTASSGAYWRGSEKNPMLQRIYGVS
                     YPDPALLDDFLRRMEEAKRRDHRRLGKELKLFTFAEEVGRGLPLWLPKGATVRRILER
                     YIVDLEQKKGYQHVYTPPLANVELYKISGHWDHFRENMYPPLVIEEEELVLRPMNCPH
                     HIMIYKSEPRSYRDLPVRLAELGEMFRYERSGVLTGLHRVRGMVLNDAHIFCAPEQIK
                     KEFQNVVRLILEVYQDLQITDFWHRLSLHDPQDRVKYAQNEEMWRVAEGMLREALDEL
                     GLPYVVAPGEANFYGPKLDVQIKTATGQEETLSTVQLDFHLPERFGLEYAGADGQPHR
                     PVMIHRAIISTLERMTAFLIERYVGAFPLWLAPVQVAVLPITDAQVDYARFIHERLVG
                     EGFRAVLDLGNEKIGYKIREAEVQKVPYMFIVGKKETEAGTVALRRHGQGDLGAKVLE
                     SAIAVLQAEVETKAVGPPGEKLEVSKRRETP"
     gene            4507..5142
                     /gene="infC"
                     /locus_tag="AC16GM_06020"
     CDS             4507..5142
                     /gene="infC"
                     /locus_tag="AC16GM_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR2923267.1"
                     /translation="MKGERELRVNYRIRIPQVRVIDYDGKQLGVMSTRDAQRLANDRG
                     LDLVEVAPTVRPPVCRIMDYGRYKYEQNKKARTTKKKQHVTQVKEMHFRPKTDEHDYR
                     FKLKHVREFLEEGDKVKVSVDFRGREMAHQEFGHKLVERLVKDVADTGQVEQRAKLEG
                     RNLILLLAPFHRRAHSPARATEGTESPLRPQTSRTSGTTHAADEPIPTEGT"
     gene            5153..5347
                     /gene="rpmI"
                     /locus_tag="AC16GM_06025"
     CDS             5153..5347
                     /gene="rpmI"
                     /locus_tag="AC16GM_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR2923268.1"
                     /translation="MPKLKTNRSAAKRFRKTAKGRFKHSQAGRRHILSSKSAKRKRQL
                     RGAAYVEPVDEKKLKRLLPY"
     gene            5360..5710
                     /gene="rplT"
                     /locus_tag="AC16GM_06030"
     CDS             5360..5710
                     /gene="rplT"
                     /locus_tag="AC16GM_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR2923269.1"
                     /translation="MPRATNNPAARRRKKKILKQARGYFGGRSKLYRTAREAVQRGLA
                     YAYRDRRARKRDFRRLWIVRINAAARLYGLTYSLFINGLHKAGVALDRKILADLAVRD
                     QATFAKLAELAKSA"
BASE COUNT         1237 a         1706 c         1753 g         1097 t
ORIGIN      
        1 tggtgacgct ccggagcgag ctgcaccgcc ttcggtctcg agcgatgcaa tcgtctccgg
       61 tcgctccggg ctctggctcg gcgtgcaagt cgccgattgc ttgccgattt ttctggtcga
      121 tccggccaaa cgagtcgtca ccctcattca cgccggctgg cgcagtactt ttctcggagt
      181 gattcccaaa acgcttttga ccctgggcga ctcctttgct tccacccctg accgtctagt
      241 ggcggtcctg gggccgacct tgcagtcctg ctgcgcggaa gtcggcccgg aagtagccct
      301 cctctttccg acgaaaaatc gggttgcccg gaacagtgcc tgggtggtag atttccccgg
      361 gatggtgcgg gagcaattgc ttgcgggagg tctctcatcc gggaagattt ttgttttacc
      421 cgtctgcacc cggtgcggcg gtgaattgtt ttgctcctac cggcgggagg ggagggaggc
      481 cgggcggatg ttcgcctatg cagggtttgt tcaaaaataa ggagcggtca aggacacccc
      541 cgcttattcc gatactaact gatagccgct ctggcagtta ggccaagagc aaccaagagg
      601 agggaagatg ctgaaaaaga aaatggttct ctggggaagt ctcatcactg cgggagttat
      661 cttgtccgcc tcactgcaag ccgtagctgg tatgggaatc ggcgtacgcg gcggatgggt
      721 caacggctac aaaaaccctg ccgtagcagg tcaattagct ccggcccaga ccagtctgcc
      781 caagcagatg cagatggtcg gcctgcacgt caagctgggc accgtgcccg ttctggacct
      841 ggagggctct ctggagtatg cctggaagga gaaaaagttc acctacctgt ccaataacta
      901 caacttccgg ctctcagata tttccgccaa tgccaccgca gagtacgttt tgcccttccc
      961 ggtgctcaaa ccgcatatcg gtgcgggttt gggactgcac cgcatcgtct actcggtcaa
     1021 ggatccgcaa gcaccgtacc cctttcccct gccgggtgat gggaccaaag tcggttacca
     1081 cgcgctcgtg ggagtgacgt ttcacccgcc cctgtttccg ctggaattct tcggcgactg
     1141 gcggtacacc tgggtcacca ccaccggcaa gcagacgaga tacagcaccc ttttggccgg
     1201 ggtaacttta gggttaccgt aagcgccccg caagctgcca cctcgctgag agttcttact
     1261 taccgcctgg tcatggcggg cggcaccata ttgtcttttc cggatatctg acgttcccaa
     1321 cctggtcacc gccggtggtg acgtaattcc tgtccgagtc atcctcggga aaaaaagtaa
     1381 aggccgtcac ccccggcggg catttgtatt gacaagccag gatcaatcag gtatacttat
     1441 ccgccagatt tgtgtggttg gcttttgtga agcgtgcgga acgaggcaaa ggagtctctc
     1501 cggtcggcag gagccaggtg acgtcgttga aaggagcgaa ttaatgttgc gtacgatgag
     1561 attcggtgtc gtggcattcg tggcgtgcat tggaatggcc cgggcacaga aagatcaatt
     1621 cggcgtggtc gataccgttt cggtggctgt ggagaagggg aaaggcgaag ggaactgggt
     1681 ggcgacggtg gctttggcca acgacgaaaa attgcaggga cttacggtgc ctttgaaata
     1741 cccggagggg tgcaaggtcg actcggtcag ttttgccgac acgcgactcg tttccttctg
     1801 gatgaaagta ggcaatacca acaccagtgt ccagaacgcg gtgctccttt tcggcatcgc
     1861 caccgccgac cagaagaagc agtacctgga gccgggccgc gggcccattg cccgcatcta
     1921 tttttcggcc cccagggaac tggcgatgac cgccgccagc ctggatacga cgcggctccc
     1981 cttgcacaat gtgctgcaac tggtcactcc ggacagcaag agcatctacc ccgcttttgt
     2041 caacggtgcc gctgtttcag cacctgccgt cgtaccggca gcggccagta ccgataccgc
     2101 tgccgccaag aaggcgccgg ccaagactgc gacgccgacc aagtcgaaaa agaagaagta
     2161 agttggcggc cctttcctgc gaaccatttt ttccggcacc ccgttctgcc gcgtcatctc
     2221 gtttccaggg gagtgtgcct gttgcggttg tgggaatgtc gccgcagcgg tcgggggcga
     2281 ttagctcagt tggttagagt gccgtcttga catggcggag gtcactggtt cgatcccagt
     2341 atcgcccacc attgctgcac gaacggaatg gcggtggtta aggcaataat tcccgcgacg
     2401 ataggaatga gtgaagagcc cggggtcgac cgggattatg gctacgggag aaatccgcgt
     2461 tagtacacaa agatcccggc acatgttttt atctatggtg aaggagttgc agacaatccc
     2521 agccggtagt aacaagataa tcgtgactgt cggcagaaac tcaccgcagc agtacccgcg
     2581 gggtatcacg cccgcggaga ttttacagcg cattcgccat cccaaaccag aggcaatctt
     2641 ggcggccaag gtcaacggtc gggtggtgga cctgaaccgg ccgctggctg ccgacagcac
     2701 ggttgaattt ctcaccttcg acgaagcgga aggccgggaa gtcttctggc actcctccgc
     2761 ccacgtcatg gcccaggcgg tcaccgaact cttccccgga acgaaactgg ccatcggccc
     2821 gccgatcgag gaaggcttct actacgattt cgaccgcggc gaaccgtttt cccccgagga
     2881 cttggaacag atcgaaaagc ggatgcgtaa aatcgtagcc ggcgcctacc cgttccagcg
     2941 caccgagtgc tcacgggaag aggcgcttga gcgcttcggt cctctggggg agaagtacaa
     3001 actggaaatt ttagaggcga taccgcccga tgagcaggtc agtctctaca cgcacgccac
     3061 ctttaccgac ctctgccggg gaccgcatct gcccgacacc ggggggataa aagcggtgaa
     3121 gttaaccgca tcctccggcg cctactggcg cggctccgag aagaacccga tgctccagcg
     3181 gatttacggc gtttcgtatc ccgatccggc gcttttagac gatttcctgc ggcggatgga
     3241 agaagccaaa aggcgcgacc accgccggct gggcaaagaa ttaaagcttt tcacttttgc
     3301 cgaagaagtg ggtcgcggcc tgccgctctg gctccccaag ggggcgacgg ttcggcgcat
     3361 cctggaacgg tacatcgtcg acctggaaca gaaaaaaggc tatcagcacg tctacacgcc
     3421 gcctttggcc aatgtcgaac tgtacaagat ctccggccac tgggaccact tccgcgagaa
     3481 catgtacccg ccgctcgtca ttgaagagga ggagttagtc ctccggccga tgaactgccc
     3541 gcaccacatc atgatttaca aaagcgagcc gcgctcctac cgcgacctgc cggtacgcct
     3601 ggccgagctg ggcgaaatgt tccgctacga acgctcgggc gtcctcaccg gcttgcaccg
     3661 ggtgcgcggg atggtgctca acgacgccca cattttctgc gcgcccgaac agatcaaaaa
     3721 ggaatttcag aacgtagtgc gcctcatcct ggaagtctac caggatctcc agatcaccga
     3781 tttctggcac cggctgtcgc tccacgatcc ccaggacagg gtgaagtatg cccagaacga
     3841 agagatgtgg cgggtggccg aagggatgct gcgcgaggcc ttggacgaac tgggactccc
     3901 ctacgtcgtt gcccccgggg aggccaactt ctacggaccg aaattagacg tccagatcaa
     3961 aaccgccacc ggccaggagg aaaccctctc caccgtccag ctcgacttcc acctgccgga
     4021 acgctttggc ctggagtacg ccggcgccga tggccagccc caccgaccgg taatgattca
     4081 ccgggccatc atcagcaccc tggagcggat gaccgccttt ttgatcgaac gctacgtcgg
     4141 ggcattcccc ctctggctgg cgccggtgca ggtggccgtt ctgcccatta ccgacgcgca
     4201 ggtggactac gcccgcttca ttcacgagcg gctcgtcggt gagggattcc gcgccgttct
     4261 cgatctcggc aacgagaaaa tcggctataa aatccgggag gcggaagtcc agaaagttcc
     4321 gtacatgttc atcgtgggga aaaaagaaac tgaagccggg accgtcgccc tgcggcgcca
     4381 cggccagggt gacttaggcg cgaaagtgtt ggagtccgcg atcgcagtcc tccaggccga
     4441 ggtagagacc aaagcggtcg gaccaccggg ggaaaagttg gaagtgtcca aaaggaggga
     4501 aacaccataa aaggagagcg ggaactgcgg gtgaactacc ggattcggat tccccaggtg
     4561 cgcgtcatcg actacgacgg caagcagctg ggggtgatga gcacgcgcga cgcgcagcgg
     4621 ctggccaatg atcggggact ggatttagtg gaggtggcgc ccaccgtgcg gccgccggtc
     4681 tgccgcatta tggattacgg ccggtacaag tacgaacaga acaagaaggc ccggaccacc
     4741 aaaaagaagc agcacgtgac gcaggtgaag gaaatgcact tccgacccaa gaccgacgag
     4801 cacgactacc gcttcaagct gaagcacgtg cgggagtttc tggaagaggg ggataaggtc
     4861 aaagtttccg tcgacttccg cggccgggaa atggcccacc aggagttcgg ccacaagctg
     4921 gtggaacgtt tggtgaagga cgtggccgac accggccagg tggagcagcg cgccaaactg
     4981 gaaggacgca acctgatttt gctcttggcg ccgttccacc ggcgggcgca cagcccagcc
     5041 cgggccacag agggaaccga atcaccgctg cgacctcaga cgagccgtac gtccgggacg
     5101 actcacgccg cggacgaacc gatacccacc gagggtacct aaggggaaaa ctatgccgaa
     5161 actcaagacg aaccggagcg ccgccaagcg ctttcggaaa accgccaagg gccgttttaa
     5221 gcacagccag gccggacgcc ggcacatcct ctcttccaag tctgccaagc ggaaacgtca
     5281 gctgcgcggg gcggcttacg tggagccggt tgacgaaaag aaattgaagc gtttattacc
     5341 gtactgagag gaccattcga tgccacgcgc tacgaacaac ccggcggccc gacgccggaa
     5401 gaagaagata ctcaagcagg cccggggcta ctttggcggc cgcagcaagt tgtaccgtac
     5461 cgcccgcgaa gcggtgcagc gcggcctggc ctacgcttac cgcgaccggc gcgcccgcaa
     5521 gcgtgacttc cgccggctct ggattgtgcg gatcaacgcc gccgcccggc tgtacggttt
     5581 gacctacagc cttttcatca acggtttgca caaagccggt gtcgccctcg accggaagat
     5641 tttggccgac ttagccgtcc gcgaccaggc gacctttgcc aaactggccg aattggccaa
     5701 gagtgcttag agggaatcgt ggctgacgtt tggggcacag gccaatccgc ctcaggcgga
     5761 ttggcctgtg ccctttgtca tttgggtaag tca
//
LOCUS       DBNGZD010000233         3192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_523904, whole genome shotgun sequence.
ACCESSION   DBNGZD010000233 DBNGZD010000000
VERSION     DBNGZD010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_523904"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="AC16GM_06035"
     CDS             complement(<1..656)
                     /locus_tag="AC16GM_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923270.1"
                     /translation="MPEERRPHWWSAPVKAILLAVIIFFIAKTLLANWRQVRTFPWRF
                     NIPLLLSATVLQYSVLLYLIEVWRRVLAYFGGRLSFRAAYRIWVVANLGKYLPGKVWS
                     VVGLATLAQRAGVTAELTVAVAMLTSALTVLGAFVVVFLFSGTSHFAGIPPSVYFVFV
                     SICLVAVYPRWLFRLLNLALGFLKRDPIRLAVPPGLPFVLLIFYLVAWVLYGGSFFLF
                     L"
     gene            complement(750..1847)
                     /locus_tag="AC16GM_06040"
     CDS             complement(750..1847)
                     /locus_tag="AC16GM_06040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2923271.1"
                     /translation="MPNYPSRPRFRHQSHQPRFSVVVPAYNEAENIPILVEKFVETFR
                     QNRLFGEVVIVDDGSTDRTYLRAREYQEKFRYIRVISHKTNMGLTAALQTGFQAARAD
                     VLVFYPADLQYLPEDLPKLLNRLNEGYDIVTGWRQGHYGLKRFVSFVYNLLSRLLFRM
                     KVHDLNSIKAFRREVIEAIPFRRDWHRYFVVIAAERGFKISEVKVRLYPRKYGKSKFG
                     FWRIPVGMLDLIAVKFQISFMQKPLLFFGSIGLFLLGLAVLVGVVAVILRFGLDEGYR
                     PLLYLVMLLASIGMVNFALGFLAEGLQIIREKLEDLEKKLPATPAAVVQSPPQLPRPR
                     PQMRPQPQFPQPRPPAQEPQAPPGEPETMQS"
     gene            1975..3036
                     /locus_tag="AC16GM_06045"
     CDS             1975..3036
                     /locus_tag="AC16GM_06045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR2923272.1"
                     /translation="MRATTETAINEIRAALSDLWDISVIYTTPAELVIQGQPRQSLEL
                     DGVTTRARLAALGYQVLIEPHPDQTLLILRRTEATAARRIPWVNIILFFATLLTTSLM
                     KPLTQPGVSLSDRPRLILEWLPYSGALLTILVCHEFGHYFMSKARGVQTSLPYFLPAP
                     TLFGTFGAFIKSKSYFRNRSDLLAIGAAGPIAGFIVALPVLFIGLSQAKIVPAGGGGM
                     NFGESLLTWAIASITHGPLPKGTALEAGPLLLAGWVGVLVTMMNLLPLGQLDGGHILY
                     GLLGKKQVTIARWAFLALLPLGLLWSGWWFWGALALVFRLRHPPTLDDTAPLDFPHKL
                     MAFACGLIFILCFIPVPFS"
BASE COUNT          719 a          944 c          894 g          635 t
ORIGIN      
        1 aggaagagaa aaaaagaacc gccgtagagg acccaggcta cgaggtaaaa aatcaggagc
       61 acaaacggga gtcccggcgg cacggccagc cgaatcgggt cacgtttgag gaaaccgagc
      121 gcgaggttga gcaaccgaaa gagccagcgc gggtagaccg ccacgaggca gattgagacg
      181 aaaacgaaat aaaccgacgg tggtatgccc gcgaagtgac tcgtaccgga gaaaaggaac
      241 accaccacaa acgctcccaa aacggtcaaa gccgaggtca gcatcgccac cgccacggtc
      301 aattccgccg tcactcccgc cctttgcgcc aacgtggcca gacccaccac cgaccagact
      361 ttccccggca ggtacttccc cagattggca accacccaga tccggtacgc cgcccgaaag
      421 gaaagacggc cgccgaaata ggcgagaacc cggcgccaaa cttcgataag gtagaggagg
      481 acggagtact ggagaacggt agcgcttaaa agaagcggaa tattgaaccg ccagggaaaa
      541 gtccgcacct gccgccagtt ggccaaaagg gtcttggcga taaaaaaaat aattaccgcc
      601 aaaaggatgg ctttgacggg cgcagaccac cagtggggtc gcctctcctc aggcatgggc
      661 ggctacccct atcccggtga agatcgtcgg cagccgccgg caaactccct ccggcccgca
      721 attggccgcg ccgaacagtc ggacatgcgc tatgactgca tcgtctccgg ctcacccggc
      781 ggcgcctgtg gctcctgcgc cggaggccgc ggctggggaa attggggctg gggtctcatc
      841 tgcggccgtg gccggggcag ctgcggggga gattgaacca ccgcggctgg cgtagcgggc
      901 agtttcttct ccagatcttc taatttttcg cggatgattt ggagtccttc ggccagaaaa
      961 ccgagcgcaa aattgaccat accgatggag gccagaagca tcactaagta aaggagcggc
     1021 cggtaccctt catcgagacc gaaacgcaag ataaccgcca ccacgcccac cagcaccgcc
     1081 agtcccaaaa ggaacagccc gatagagccg aagaacagaa gcggcttctg catgaacgaa
     1141 atctggaact tcacggcaat cagatcgagc atccccaccg gaatccgcca gaagccgaat
     1201 ttcgacttcc cgtacttgcg ggggtagagc cgcactttaa cttcgctgat tttgaaacct
     1261 ctctctgccg ctatgaccac gaaataccgg tgccaatccc gccggaaggg aatcgcttca
     1321 atcacctccc gccggaaggc cttgatcgaa ttcaaatcgt gaactttcat gcgaaagagt
     1381 agccgggaca aaagattgta gacgaaggag acgaagcgtt tgagcccgta gtgcccctgc
     1441 cgccagccgg tgacgatgtc gtacccttca ttcaagcggt tgaggagttt ggggagatcc
     1501 tcgggcaggt actgcaggtc agccgggtaa aagaccaaaa cgtcggcccg ggccgcctga
     1561 aagccggttt gcaaggccgc ggtcaagccc atattagttt tatgggagat cacccgaatg
     1621 tagcggaatt tttcctggta ctcccgggcc ctgaggtagg tgcggtcggt ggagccgtcg
     1681 tccacgatca ccacttcgcc gaagagccgg ttctggcgga aggtctcgac gaacttctca
     1741 accaagatcg ggatgttttc cgcctcgttg tacgccggca ccactactga gaaacggggc
     1801 tggtgactct ggtgacgaaa gcgtggtcgg gagggatagt taggcatgat tctgtatctc
     1861 agccaccggg ctggacttct ctatataaag cacgaggtaa gccgccgtca agatacccga
     1921 ccgaaagaaa atcggatact gcgcaacccg tagctgtatt atcttactca cacgatgcgc
     1981 gctaccacgg aaacagccat aaacgagatc cgggcggctc tgtccgacct ctgggacatt
     2041 tcggtcatct acacgacgcc cgctgaactc gtgatccaag ggcaaccccg acagagtctg
     2101 gaattggatg gagtcacgac gcgggctcgc ctcgctgccc tgggctatca ggtcttgatt
     2161 gagccgcacc cggatcaaac cttgctcatt ctccgccgga ccgaagccac ggcggcgcgc
     2221 agaattccgt gggtcaacat catcctcttt tttgcgacct tgttgacgac ttcgctcatg
     2281 aaacctttga cgcagcccgg ggtctcccta tctgaccgac cgcgcctgat tctcgaatgg
     2341 ctcccttact ctggagcact cttgaccatt ttggtctgcc acgaattcgg gcactacttt
     2401 atgtcaaagg cgcggggtgt acagacctcc ctgccctatt ttcttccggc gccgacatta
     2461 ttcggaacat tcggtgcttt catcaagtcg aaatcgtatt tccgcaaccg gagcgacctc
     2521 ttagccatcg gcgccgccgg gccaattgcg gggttcatcg tcgctttacc ggtgctcttt
     2581 attggtttga gtcaggcgaa gattgttcct gccggcggcg gaggcatgaa tttcggcgag
     2641 tcccttttga cctgggcgat cgcctctatc acccacggcc cgctccccaa gggcaccgct
     2701 ctcgaagccg gcccgcttct tttggccggc tgggtgggag ttctcgtgac gatgatgaac
     2761 ctgctcccct tagggcagtt ggacggcggc cacatcctct acgggctttt gggaaaaaaa
     2821 caggtgacca ttgctcgctg ggctttcctt gcgcttttgc ctctgggttt gctctggagc
     2881 ggctggtggt tttggggcgc tctggcttta gtcttccgcc tgcgtcaccc accgactctt
     2941 gacgacaccg ctccgctgga cttcccccac aaactgatgg ccttcgcctg cggcctcatt
     3001 ttcatcctct gcttcatccc ggtgccgttt agttgagggg ggatagttac ccgcgtaggc
     3061 ggagcaccgg gtttgaaaac cccacgcgac tcagaataaa ttaaattcta agtcagcatt
     3121 tggtaaatcg aactgcagtt tactatttca gacataccgg tctctacagg gaaagtacta
     3181 cggttgcgtt cg
//
LOCUS       DBNGZD010000234         6914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_800980, whole genome shotgun sequence.
ACCESSION   DBNGZD010000234 DBNGZD010000000
VERSION     DBNGZD010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_800980"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1312)
                     /locus_tag="AC16GM_06050"
     CDS             complement(14..1312)
                     /locus_tag="AC16GM_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923273.1"
                     /translation="MQDRRLIQNLMVTGAIFCLLGVATLAFGQATGEKAKQQRLSPAG
                     FDAVISAHSQQMLREGKRIFRFDTFGDEAFWGDALKLHQAIEGEKQGGVGPGVSPKTA
                     LSVGLKVDVDALPPALVEQLKAGKVDLDDPATTLALLKLNAVVGVTGFFNEKGNLRSI
                     GIQCASCHSTVDNSFAPGIGHRLDGWANRDLNVGAIVNLAPDLGVVTSLLGVDEATVR
                     KVLQSWGPGKFDAELFMDGKALRPDGKSAATLIPPAFGLAGVNLHTWTGWGSVTHWNA
                     FVANLEMHGKGTFFDPRLNDPAQFPVAAKAGFGNVRNTPDLVSSKLAALHFYQLAIPA
                     PAPLQGSFNAGAAKRGEAVFNGKAKCAGCHVPPLFTEPGWNMHTAAEVGIDDFQANRA
                     PDKRYRTAPLKGLWSHQNGGFFHDGRFANLKEIICSPVGR"
     gene            complement(1510..1773)
                     /locus_tag="AC16GM_06055"
     CDS             complement(1510..1773)
                     /locus_tag="AC16GM_06055"
                     /inference="COORDINATES: protein motif:HMM:NF017847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HIR2923274.1"
                     /translation="MGLISFFTASEPEVKAWAIPRGTTALKAAGTVHSDMERGFIRAE
                     VVAVATLRELGDLHKVKEKGLLRLEGKEYPVQDGDLIHFRFHV"
     gene            complement(1783..2595)
                     /locus_tag="AC16GM_06060"
     CDS             complement(1783..2595)
                     /locus_tag="AC16GM_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR2923275.1"
                     /translation="MQIGIIGLPFVGKTTVFNAVTAQARAVTSHGPAEKVHHGVIKVP
                     DSRLDFVAGLFHPRRVTPAEIEYLDVAGITGETHEKKTVEHEIPPALHEVAALAQVVR
                     TFSDPAILHISGSVDPRRDIEKLDLEIFLADLVTLEKRMERVKHGLKMGKKEFQAEFD
                     LLSRCQKALEQNRPLRDLDLTSEESHQLSGFGLLSIKPMLYLFNIDETDINGGPEVVA
                     PFADLTRHRHTIVYPLCAKIEMELAQLPDGDRPAFMEAYGIKESALDSLIHL"
     gene            2756..3853
                     /gene="gcvT"
                     /locus_tag="AC16GM_06065"
     CDS             2756..3853
                     /gene="gcvT"
                     /locus_tag="AC16GM_06065"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR2923276.1"
                     /translation="MADELKKTPFYNLHVQSGAKIVPFAGFLMPVQYRGITEEHRSVR
                     SSVGLFDLSHMGEFVVTGQDALAFLQKMTVNDVAALAPCQVQYSCMCYPEGGIVDDLL
                     VYRLPDHYWLVVNASNIAKDFGWLKEHLSGDVQLRDISDQTGLLAIQGPKAEPLLAKL
                     TDYPLATLKFYWAAEGIIAGEKVLFSRTGYTGEDGFEIYCKPEQAEKLWQAAMEAGDE
                     FDVEPIGLGARDSLRLEMKYMLYGNDIDRTTNPIEAGLGWIVKTDKGDFIGREVIVQA
                     RQQGVRKLVAFEMTEKAIPRHGYEINATGQRIGQVTSGTFSPSLEKGIGLGYVPKQFA
                     APGQWFGVAIRDRIVPAVVVQPPFWKQYSHK"
     gene            3875..4651
                     /locus_tag="AC16GM_06070"
     CDS             3875..4651
                     /locus_tag="AC16GM_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR2923277.1"
                     /translation="MQIDLFFTPAEVSEADLANKTAVVIDVLRASTTVAWALFNGARE
                     IIPVDTIGAASELKQKLGKDHVLLAGEREGVKIDGFDLGNSPSEYQAPVVKEKIVVYC
                     STNGSKAMLKAGQAGLCLIGGLVNLSAVLEQVLASGRDVVLVCSGTNGKFSLEDAVCG
                     GFFVSELRRKLRQDCAENDAAGVALSIYHQLGKDLLATLKSSAHGKYLVRLGFEQDLQ
                     TAAEVDSLSVVPVHEAGRFVWRAEKAEPAAAPREFPVGAR"
     gene            4667..6007
                     /locus_tag="AC16GM_06075"
     CDS             4667..6007
                     /locus_tag="AC16GM_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HIR2923278.1"
                     /translation="MKFFSNLPVTLSNFSQKWVPGSFIIALLLTLFTIAMALVVAHQP
                     PLKVIEFWGDGFWELLSFAMQMCLIILTGYIVASSPPVDRLFRAAAELARGPKSAILL
                     MTAVSLLLSWLHWGLSMVGAAIFVRHLARRDLEVDYRLLVACAYLGMGCTWHAGLSAS
                     APVLVATPGHFLEKAVGIIPTSLTIFSPFNLLLVGFIFAFLCFFTPLLHPKPEETRRV
                     DPSLLEQFQQQPVFSAPKKDSLSVILEYSPLVNLIFAAAGLVWLLVHFSGKRFDLTLN
                     VLNFTFLFLGILLHWTPARFLRSAEEGGKLIFGVVLQFPFYAGMYGIIKSSGLADIVG
                     NWFVSISSQKTYPAVIYWYSGLINYFVPSGGSKWAIEAPYILAAAKSLAVPAHKVVVA
                     YAWGDMATDLIQPFWAIPLLTVARLEFKDIMGYLMVIFLVYLFLVSAAFLLFPL"
     gene            complement(6086..>6914)
                     /locus_tag="AC16GM_06080"
     CDS             complement(6086..>6914)
                     /locus_tag="AC16GM_06080"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR2923279.1"
                     /translation="AAERALTMAKVRPEELDLILVATVTGDMPFPSTACLVQKNLNAL
                     KAVPFDLSAGCTGFIYGLATADAFIRAGVYQKVLLVGVEILTKITDWTDRNTCVLFGD
                     GAGAVVLTAKSHVATHHNGQAGLLGTFLAGDGRLGNLLMMPGGGSLYPATHASVDQKL
                     HFLKMSGNDVFKLAVTEMGEAATTVLEKTGIRPEQIDLLVPHQANLRIITATAKRLKL
                     PMEKVYVNIDRYGNTSSASIPIALDEIRRSNRYHPNQLLLLVAFGAGFTWGSALVRW"
BASE COUNT         1385 a         1913 c         2071 g         1545 t
ORIGIN      
        1 ggcgagcagg ctgtcaacgt cctacgggtg aacaaataat ttccttcaga tttgcaaaac
       61 ggccgtcatg gaagaaaccg ccgttctggt ggctccagag tcccttcagt ggcgccgtcc
      121 ggtagcgttt gtccggcgcc cggttcgcct ggaaatcgtc gatacccacc tcggcggccg
      181 tgtgcatatt ccagcccggc tcggtgaaga gcggcggcac gtggcaaccg gcgcacttgg
      241 ccttgccgtt gaagaccgct tccccacgtt tcgctgctcc tgcgttgaag ctcccctgca
      301 ggggtgctgg cgcaggaata gccagttgat agaagtgtag cgcggccaat tttgaggaga
      361 ccagatcagg tgtattccgc acgttgccga aaccggcttt cgccgccacc gggaattggg
      421 ccggatcgtt cagccggggg tcaaagaagg ttcccttgcc gtgcatttcg agattagcca
      481 caaaagcgtt ccagtgggtg accgagcccc agccggtcca ggtgtgcagg ttcacgccgg
      541 ccagaccgaa agcgggcggg atcagggtgg cggcagactt cccgtccgga cggagcgctt
      601 tgccatccat gaaaagctcg gcgtcgaatt ttcccggccc ccagctttgg agcacttttc
      661 tgaccgtggc ttcatcaaca cccaaaaggc tcgtgaccac acccaagtca ggcgctaagt
      721 tgacgatggc gcccacatta aggtcccgat tggcccagcc atccagccgg tggccgatgc
      781 cgggagcgaa cgagttatcc acggtggagt ggcacgaggc gcattgaatg ccgattgagc
      841 gtagattgcc tttttcgttg aaaaagccgg tgacgcctac gactgcgttc agtttgagca
      901 gagccagcgt ggtggccgga tcatcgagat cgaccttgcc ggctttcagt tgttcaacca
      961 gagccggggg aagagcatcc acatcgactt tcaatccaac ggacagtgcg gtctttggac
     1021 tcacacccgg ccccacgcct ccctgctttt caccttcgat ggcctgatgc agtttgagcg
     1081 catcgcccca gaaggcttca tcgccgaacg tgtcgaagcg aaagatgcgt ttaccctccc
     1141 ggagcatctg ctgggagtgg gcgctaatca cagcgtcaaa tccggcggga gacagccttt
     1201 gttgcttggc cttctcgccg gtcgcttggc cgaaagccag ggtggccaca ccgagaagac
     1261 aaaagattgc cccggttacc atgaggtttt ggatgagcct tcgatcttgc atgattctgg
     1321 tctcctttcc aacatagcgg ttatgcgggt gactgcgatt gctaataata taaaccagta
     1381 gttttgtcgc taactaataa tagatgaccc atcacttgtc aagagaaaat tttattcgga
     1441 tgtcgagctt ttatgttttt gcggtacaat aaagtagtgg gtgttgacgc gccagtctgt
     1501 agcgtatcgc tacacgtgaa agcggaagtg aatcaggtcg ccatcctgca cggggtattc
     1561 tttgccctca aggcgcagca accccttttc tttcacttta tgcaggtcgc caagttctct
     1621 gagggtggcg acggcgacca cttcggcgcg gatgaaaccg cgctccatat cggaatgaac
     1681 ggtgccggcg gccttgaggg cggtcgtccc tcggggaatc gcccaggctt tcacctccgg
     1741 ctcgctggca gtaaagaaac tgatgagtcc caagatctgg tactagaggt gaatgagaga
     1801 atccagagcc gactctttaa tcccataggc ttccataaag gctgggcgat cgccgtcggg
     1861 aagttgggcc agttccattt ctattttggc acagaggggg tagacgatgg tgtgccggtg
     1921 ccgcgtcagg tcggcgaacg gggcgacgac ttcggggccg ccgttgatgt ctgtctcatc
     1981 gatattaaac aggtagagca tcggtttgat tgagagcaaa ccgaaaccgc tcagttggtg
     2041 cgattcttca gacgtaaggt cgaggtcacg caggggtcga ttttgctcca gagccttctg
     2101 gcagcgggag agaaggtcaa actcggcttg aaattctttt ttccccatct tcaggccgtg
     2161 tttcacccgc tccatccgtt tctccaaagt taccagatcg gctaagaaga tttcgagatc
     2221 gagtttttcg atatcgcggc gggggtcaac cgaaccggag atgtgcagaa tggccggatc
     2281 ggaaaaggtc cgcaccacct gagccagagc agcgacctcg tgaagggccg gcgggatttc
     2341 gtgctcaacg gtttttttct cgtgcgtctc gccagtgatg ccggcaacat ccaaatactc
     2401 gatttccgcc ggcgtcacgc ggcgggggtg gaaaagtccc gcaacaaagt caagccggct
     2461 gtccggcact ttaattacgc cgtggtggac tttctcggca ggaccatgag aggtgaccgc
     2521 cctggcctgg gcggtgacag cgttgaagac cgtggtctta ccgacaaagg ggagtccgat
     2581 gatgccgatt tgcataaaaa cacaaccgcc tttcgcatcg taatggtagg tgatttgggc
     2641 gggaagtcaa ttaccggaac ccggttatcc ggtattgatt tggccggagg ccgcgcgagc
     2701 aggcgcatgg ccgcggattg acattggagc cgggctacat attattcgcg ctgccgtggc
     2761 ggacgaacta aaaaaaactc ctttctacaa cctgcacgta caaagcggag cgaaaatcgt
     2821 cccttttgcc ggattcctca tgccggtgca gtaccgcggg atcaccgagg agcaccgcag
     2881 cgtgcgttcg tcggtgggtc tgttcgatct ctcgcacatg ggggagttcg tcgtcaccgg
     2941 tcaagacgcc ctggcgttcc tccagaagat gacggtcaac gacgtggccg cgctcgcacc
     3001 ctgccaggtg cagtactcct gtatgtgtta cccggagggt gggatcgtcg atgacctcct
     3061 ggtctaccgg ctgcccgacc actattggct tgtggtgaat gcctctaaca tcgccaaaga
     3121 tttcggctgg ctgaaagagc acctttccgg tgacgtgcag ttgcgcgaca taagtgacca
     3181 gacggggctt ttggcaattc aaggaccaaa ggctgagccc ctcttagcga aactgaccga
     3241 ctatcctctg gcgaccctca aattctactg ggcggcggaa ggaatcatcg ctggagaaaa
     3301 ggttttgttc tcgcgcaccg gctacaccgg tgaggacggc ttcgaaatct actgcaaacc
     3361 cgagcaggcc gagaaactgt ggcaggcggc tatggaggcc ggggacgagt ttgacgtcga
     3421 accgatcggc ttgggggcgc gggacagtct gcggctcgaa atgaagtata tgctctatgg
     3481 caatgacatc gaccggacca ccaatccgat cgaagccggg ctcggctgga tcgtgaagac
     3541 cgacaagggt gatttcatcg gccgggaggt cattgtccag gcccggcaac agggagtgcg
     3601 caaattggtt gcctttgaaa tgactgagaa agccatcccc cggcacgggt atgagattaa
     3661 cgccaccggg cagaggatcg gacaggtgac ctccggcacg ttttcacctt cgcttgaaaa
     3721 agggatcggt ttgggatatg ttcccaaaca gttcgccgcg ccgggccaat ggttcggcgt
     3781 ggcgatacgg gatagaattg tcccggcagt ggtggtccaa ccgccgttct ggaagcagta
     3841 ttcgcacaag tagagaggag taagcagcgt ccgaatgcaa attgatttgt tcttcacgcc
     3901 ggccgaggtc agcgaggccg atttagccaa caaaaccgca gtggtgatcg acgttttgcg
     3961 cgcctccacc acggtggcct gggcgctctt caacggggcg cgggaaatca ttccggtaga
     4021 taccatcggc gccgcctccg agttaaagca gaaattaggg aaagaccacg tccttttggc
     4081 cggggagcgc gagggcgtga aaatcgatgg cttcgacctg gggaattcgc cgtccgaata
     4141 ccaggcaccg gtggtgaagg aaaaaatcgt ggtctactgc tccaccaacg gctccaaggc
     4201 aatgctcaaa gcggggcagg ctggtctgtg cctgatcggc gggctggtca acctgagtgc
     4261 ggtgctcgag caggttttgg cttccggtcg cgacgttgtg ctggtttgct ccggcaccaa
     4321 cggaaagttt tcgctggagg acgcggtctg cggcgggttt tttgtatccg aactgaggag
     4381 aaaattgcgc caggattgtg cggaaaacga cgcggccgga gtggcgcttt ccatctacca
     4441 ccaactgggg aaagatctcc tggccacgct caagtcgtcc gcccacggga agtacctggt
     4501 gcgcctgggg tttgagcagg acctgcagac ggcagcggaa gtagatagtc tctcggtggt
     4561 gccggtgcac gaggcgggac ggttcgtgtg gcgggcggag aaggccgagc ccgccgcagc
     4621 gccgagggaa tttcccgtcg gggcacggta gcctacattt cccggaatga aatttttcag
     4681 caacctgccc gtcaccctct cgaatttttc acagaaatgg gtgccgggct cgtttatcat
     4741 tgcgcttttg ctcaccctct tcactattgc catggctctg gttgttgccc accagccgcc
     4801 tttgaaagtt atcgagttct ggggcgatgg cttctgggaa cttttgtctt ttgccatgca
     4861 aatgtgtttg atcattctga ctggctacat agtcgcttct tccccgccgg tggatcggct
     4921 ctttcgggcg gcagccgaac tggcccgggg ccccaagtcg gccattctct tgatgaccgc
     4981 cgtgtcgctt ttgctctcct ggctgcactg gggactctcg atggtggggg cggcgatttt
     5041 cgtgcgccac ctcgcccggc gcgacctcga agtcgattac cggcttttgg tcgcctgcgc
     5101 ctacttaggg atgggctgca cctggcacgc cgggctatca gcctcggcac cggtcttggt
     5161 cgccacgccg ggacattttc tggaaaaggc ggtcggcatc attcccacga gtctcaccat
     5221 cttttcgccg ttcaatttat tgctggtcgg ttttatcttc gctttcctct gcttcttcac
     5281 gccgcttctc caccccaagc cggaagagac ccggagggtt gatccctccc ttctggaaca
     5341 gttccagcag cagccggttt tctcagcgcc caaaaaagat agtctctccg tgatcttaga
     5401 atattcgccc ctcgtcaatc tcatttttgc agcggcggga ttagtttggc ttctcgtcca
     5461 tttttccggc aagcggttcg acctcacctt gaacgtcctc aacttcacct tcctctttct
     5521 gggaattctt ctgcactgga cgccggcgcg cttcctccgc tcggccgagg agggcgggaa
     5581 gttgattttc ggcgtggtgc tgcagtttcc cttctacgcc ggcatgtacg gcatcatcaa
     5641 gtcgtccggg ttggccgaca ttgtcggcaa ctggttcgtc tcaatttcga gccagaaaac
     5701 ctacccggcg gtgatctact ggtactcggg gctcatcaac tacttcgtcc cttccggcgg
     5761 gtccaagtgg gcgattgaag ctccgtacat tctcgcggca gccaagtcac tcgccgtccc
     5821 ggcgcacaag gtggtggtgg cctacgcctg gggcgacatg gcgaccgatc tcatccagcc
     5881 cttctgggcg attccgcttt tgaccgtagc cagattggaa ttcaaagaca tcatgggata
     5941 ccttatggta atttttctgg tttatctctt tctggtgagc gcggcgtttc ttttgttccc
     6001 actttgaagc aggtcggctt aacaacatta gaagagccac gctcaagaga gcgtggctac
     6061 acgcggggtt atgagtcctc aggacttacc agcgtaccag ggccgaaccc caggtgaagc
     6121 cggcgccgaa agcgaccaga agcaagagtt gattgggatg gtaacggtta ctgcggcgga
     6181 tttcgtccag ggcaatcgga atcgaagccg aggaggtgtt gccgtagcgg tcgatattga
     6241 cgtagacttt ttccatcggc agtttcagcc gcttggccgt ggcggtaatg atccgcaaat
     6301 tggcctggtg cggaacgagc agatcgatct gctctggccg gatgccggtt ttttccagga
     6361 cggttgtggc agcctcgccc atctccgtca cggccagttt gaaaacgtcg ttccccgaca
     6421 ttttcagaaa atggagtttc tggtccaccg aggcgtgcgt ggcggggtag agcgaaccgc
     6481 cgccgggcat cattaacaga ttcccgagcc ggccgtcacc ggctaaaaag gttcccagga
     6541 gtcccgcctg gccgttgtgg tgggtggcga catgactttt ggcagtgagc accacggcac
     6601 cggcgccgtc gccgaacaga acacaagtgt tccggtcagt ccaatcggta atcttggtca
     6661 aaatttctac gccaactaag aggacctttt ggtaaacgcc tgcccggatg aaggcgtcgg
     6721 cagtggccag accgtaaatg aaaccggtgc agcccgccga caggtcaaat ggaaccgcct
     6781 tcagggcatt caagtttttc tgcaccaggc aggcggtcga ggggaagggc atgtcgccgg
     6841 tgacggtggc gaccaagatg aggtcaagtt cctccgggcg gaccttggcc atggtcagag
     6901 ctcgttcggc agcg
//
LOCUS       DBNGZD010000235         4076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_660759, whole genome shotgun sequence.
ACCESSION   DBNGZD010000235 DBNGZD010000000
VERSION     DBNGZD010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_660759"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..900
                     /locus_tag="AC16GM_06085"
     CDS             256..900
                     /locus_tag="AC16GM_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923280.1"
                     /translation="MKRVGILALVGLTVLGAATLAPAQQMISTMNSARVLAPGASMAG
                     GYFGVFDGGISVYGQYRYGFSLPFEGGLQFGFVDFDGDGNSGVILGGDGQFQIIDAKL
                     HDPFDLSAGGLVQFAFVGDLNIFSLGGSMAISKDLPLRSGRSFTPYGRLQLRMDRADV
                     SVNTPFGRISGSNTDFNVAFNIGGDLEIANNTDLVSELQLADNVGFLIGFNVHF"
     gene            990..1811
                     /locus_tag="AC16GM_06090"
     CDS             990..1811
                     /locus_tag="AC16GM_06090"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR2923281.1"
                     /translation="MNFGQAIILGILQGLTEFLPISSSGHLVITERLFGLKLANIVFD
                     VAVHVGTLVAVLVYYRKKLLTILRAMLSSTGFGSMASVPAGVSEKADARKLFWYLVAG
                     SLPAAVIGLSFKGFFESTFTEPRLVSAFLMVTGLVLFSTRFARAGSQRLNLWRALAVG
                     CAQAVAILPGISRSGSTIAAALLLGVAGPDAAEFSFLLSVPAVAGAAFLELRHLNGVG
                     ADSQLLFLCVAGALAAFAVGLGAIHFLVSYLKRGNLVPFGYYCLAVGGFFVYYFR"
     gene            1812..2846
                     /locus_tag="AC16GM_06095"
     CDS             1812..2846
                     /locus_tag="AC16GM_06095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR2923282.1"
                     /translation="MDLTSVKRILVIRLSSFGDLVLTQPVFANLKAACPDASVELLVR
                     EKYAPLFEPGPCTGGADVVHTFEQFRPSQEIFGLLFDLHGNWRSWQVRRQIRAGRVFV
                     YPKQRVARMARVWLKRFSPFRLRPTLELYFDVLRQAGIPILTSEPNWRVHESARQEAG
                     KYLTNQKAGQGDFLVGLGPGARWETKRWSAERFIETGRLLNRARPARFVLFGSKGEEQ
                     LLRQIAAELPDPKPLIISDAPLGLLPALLAELRLFISNDSGLMHLAVAVGTPTIGIFG
                     PTHPALGFAPTGKHTRTLHHPVWCSPCSLHGSKPCFRSRRFCLEGITPEQVVRVADAL
                     LNQKVATVLR"
     gene            complement(3011..3805)
                     /locus_tag="AC16GM_06100"
     CDS             complement(3011..3805)
                     /locus_tag="AC16GM_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923283.1"
                     /translation="MLREEFTRQTAQSVRQKTMMLFLIAVLIVLPLTQPVSQDSKTDM
                     WYENTAAAKKNMGHPRSKTSDSGLRIFKRAKSFGDWLSPSEFKFSTSHYNFNITGGGR
                     GTRKLGISDSATFQLKLDTLMCGGETYIRSIYYLESEADLILIYEMSDDESGWGSVTR
                     LNQERLSPEWTEHVPSFGIKGVVESSYVYFMASGYVAKLNLDSGKYVWQHKNLYKDYH
                     FNNFELPIIEGDVVLFKEIPQDLPKQKLRAKTIKVQKQSGKILSIH"
     gene            complement(3869..>4076)
                     /locus_tag="AC16GM_06105"
     CDS             complement(3869..>4076)
                     /locus_tag="AC16GM_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923284.1"
                     /translation="PGSGIDQAPDTAASHCGLGPACAKRIGKNDLVAYQASPRGGTLR
                     VRVAGDRVYLLGQAVTIFKGELMA"
BASE COUNT          806 a         1061 c         1179 g         1030 t
ORIGIN      
        1 cggcagggcg catcgagcga aaaatcgttt catttctctt ttcggcagga atcggcatgg
       61 cttgagacga aggacagccg ccgcctgttt gcagccactg ttagtgtgca aaaaaatgcc
      121 ttatgcgaaa aaagttgttg acagccgctc tgcgggaggt taactttcct tctgattcag
      181 ccgaactaca gtatgtgcag ccaggcaagg ggctgggtcg ataaacggag caaaacggca
      241 agagaggagg ggaaaatgaa acgggtggga atcttagcac tcgtcgggct gaccgtctta
      301 ggtgcagcaa cacttgcacc ggctcagcaa atgataagca ctatgaacag cgcaagggtt
      361 ctggcgccag gtgcatctat ggccggcggc tacttcgggg tgtttgacgg cggcatatcg
      421 gtgtacggcc agtaccgcta cggattttct ttgcccttcg aaggcgggct gcagttcggc
      481 tttgtggact ttgacgggga cggcaacagc ggggtcattt tgggcggcga cggtcagttc
      541 cagatcatcg acgccaagtt gcacgacccc tttgatctct ctgcaggggg attggtgcaa
      601 ttcgcttttg ttggagattt gaacatcttc tcgctcggag ggagcatggc gatctccaaa
      661 gacctgcccc tgcgctccgg acggagcttt acgccgtacg gccgcctgca gctgcggatg
      721 gaccgcgccg atgtcagtgt aaatactccc ttcggccgga tcagcgggag taacaccgac
      781 ttcaacgtgg cgtttaacat cggcggcgac ctggagattg ccaacaacac cgacctggtg
      841 agcgagttgc aattggccga caacgtcggc tttctgatcg gattcaacgt ccacttctaa
      901 agggagcgcc gtcggccctt gttcaactgg aatttcacct tgcgtaccgc tgaaacaggc
      961 tctggcagac cagagcctgt tttctttcga tgaacttcgg gcaggcaatt attttgggga
     1021 ttctgcaggg attgaccgaa ttcctgccga tctcctcctc cggccacctg gtgatcaccg
     1081 agcgcctctt cgggctgaag ttggcgaata tcgtctttga cgtggcggtg cacgtgggga
     1141 cgcttgtggc ggtcctggtc tactaccgga agaagttact caccatcctg cgcgcaatgc
     1201 tgagcagtac cggtttcggc tcgatggcgt ccgtaccggc gggggtgagc gaaaaggccg
     1261 atgcccgaaa actcttttgg tacctcgtgg ctgggtcact gccggcggcg gtcatcggtc
     1321 tgagcttcaa aggttttttt gagagcacct tcactgagcc acggttagtt agcgcctttt
     1381 tgatggtcac tggattggtc cttttctcca cgcgctttgc ccgcgccggt tcgcagcggc
     1441 tgaacctctg gcgggcgctg gcggtgggct gcgcccaggc ggtggcgatc ctgccgggta
     1501 tctcccgctc cggatcgacc attgcagcgg cgctcctttt gggagtggcc ggacccgatg
     1561 cggccgagtt ttcctttctg ctctcggtac ccgctgtggc gggcgcggca ttcttggagt
     1621 tgcgccacct caacggtgtg ggcgcagatt cccagctttt gttcctctgt gtcgccgggg
     1681 ccctagcggc gttcgccgtc ggactgggcg ccatccactt tctcgtctcc tatctcaagc
     1741 gagggaattt agtgccgttt ggctactact gtttggcggt gggcggattt ttcgtgtatt
     1801 atttccgctg agtggacctg acttccgtca aaagaatcct ggtcattcgc ttaagttctt
     1861 tcggtgacct cgttttaact cagccggttt tcgccaatct gaaagcggct tgtcctgacg
     1921 cttcggtcga gctgctggtg cgggagaagt acgcgccgct ctttgaaccc ggcccctgta
     1981 cgggcggcgc tgacgtcgtt catactttcg agcaattccg tccttctcaa gagatttttg
     2041 gcctgctgtt cgacttgcac ggcaattggc gttcctggca ggtgcgccgc cagattcggg
     2101 ccggaagggt ttttgtctac cccaaacaaa gggtggcgcg aatggcgcgg gtctggctca
     2161 agcggttttc tccttttcgg ctgcggccaa cgctggagtt gtacttcgat gttctgcggc
     2221 aggcgggcat tccgattctg acctctgaac cgaattggcg cgtgcatgaa tctgctcgtc
     2281 aagaggcagg taagtatttg acgaatcaga aagctggaca gggcgatttt ctggtcggtc
     2341 tcggccccgg cgcgcggtgg gagaccaaac gctggtcggc ggaaaggttt atcgagactg
     2401 gtcggttact caaccgggct cgaccagcac ggtttgtttt gtttggcagt aagggggaag
     2461 agcaactatt gcgtcagatc gcagccgagc tgcccgatcc aaaaccactg attatttcag
     2521 atgcaccgct gggtctcctc ccagcgcttc tggcagaatt acgccttttc atttccaatg
     2581 acagcggttt aatgcacctc gctgtcgcgg tggggacacc caccatcggc atcttcggcc
     2641 ccacgcatcc ggccctggga ttcgctccca cgggcaaaca cacgcgcacg cttcatcatc
     2701 ccgtctggtg ctcgccctgt agtttacacg ggagcaagcc gtgctttcga tcacgcaggt
     2761 tttgcttgga gggaattacg ccggagcagg tggtacgagt ggctgatgcg ttattgaacc
     2821 aaaaagttgc gactgttttg cgttgacctc accccgcctg aggcgcgtct tcgacctccc
     2881 tttcctacga ggagagggtg aattcctaac gtaagagtgc gtcagcgacg attttcggca
     2941 tcgtttaggt ctgacagttc aaaggcgacg atccataagg atcgccgagg ttggaaacct
     3001 cggctacgtg ctaatgtata gacagaattt tgccgctttg cttttgaact ttaatggtct
     3061 tggctctcaa tttttgtttg gggaggtctt gagggatctc tttgaataag accacgtcgc
     3121 cttcaattat gggcaattca aagttattga aatgatagtc cttgtacaga ttcttatgtt
     3181 gccagacgta tttccctgag tcaagattca atttggctac atagcctgat gccatgaagt
     3241 atacatagct gctctctaca acacctttaa tgccgaaact aggtacgtgt tccgtccact
     3301 ccggtgacag gcgttcctga tttaatcggg tgacggaacc ccacccagat tcatcatcag
     3361 acatttcgta aatcaatatc aagtcagctt ctgactcgag ataataaata cttcgaatat
     3421 aagtttcacc accacacata agcgtgtcca attttagttg gaaagtggct gaatcggata
     3481 tcccgagctt gcgagtgcca cgaccacccc cggtgatatt aaaattgtaa tgggaagtgg
     3541 agaatttgaa ttcagagggg gagagccaat ctccgaaaga ttttgctctt ttgaaaattc
     3601 tgagaccaga atcagaggtt ttgctccggg gatgccccat attcttcttg gccgcagcgg
     3661 tgttttcata ccacatatca gtctttgagt cttgcgagac gggttgagtt aggggtaaga
     3721 caatcaggac cgcgatcagg aataacatca tggtcttttg tctcaccgat tgggcggtct
     3781 gacgtgtgaa ttcttcgcgg agcatcatta gtagttatcg gaaattatcg cataattttc
     3841 agcaagacct ctttcgttcg ttctctcatc aggccattag ttcgcctttg aaaatggtaa
     3901 ccgcctggcc gagaaggtag acgcggtctc cggcgacgcg aacgcgcaga gttccgccgc
     3961 ggggggaggc ctggtaggca acaaggtcgt tcttgccgat ccgtttggcg caggcggggc
     4021 ccagaccgca gtgggaggca gcggtgtcgg gggcttggtc tatgcctgag cccggg
//
LOCUS       DBNGZD010000236         1593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_801192, whole genome shotgun sequence.
ACCESSION   DBNGZD010000236 DBNGZD010000000
VERSION     DBNGZD010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_801192"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..1510
                     /locus_tag="AC16GM_06110"
     CDS             515..1510
                     /locus_tag="AC16GM_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2923285.1"
                     /translation="MKRLGLLAAAGVLLFGGLFPLALGDDRTELLQSRDGQSNHEPTS
                     GDALDPSKRDTVRVATVDALGANVVVPVYLFNSKKRLAGSLVSLKYGSDLVTCDSVSF
                     ADTRLVASEFRVAQVRLQEQVIQFTLLSVSGHNGDGDDEGDDGNEDEDNPLTHSVPPK
                     DFGVLAFLHFRVTGAPSPSNHFAPIDTFLSVPLVVRFRGEKIDPVKHKQNAELRRATF
                     VPGGINFLTSPVTRERDGALPKSFELFQNHPNPFNPSTTLRFAVPQTSRVELAVYNIL
                     GQKIRTLLDEVVPAGVYSTEWNGTDDAGMGAPSGIYFARFAAGSRVATQKMTLLK"
BASE COUNT          358 a          430 c          456 g          349 t
ORIGIN      
        1 ggacgacgga catatgcaca agctggtggt cacgtttcag gataaagacc acttcacgca
       61 ggagtggacc tggtgggaaa aaagcaagga agcgggtaca cacgtttttc agttcgagcg
      121 taagaagtaa gtcggcattt ccgacccggg ccggttctgt tccgcccggg tcggaagacc
      181 tctccgtcgc cgacgcacgg ggggtatttt tgctaagcgg aaaattcaaa cggcactact
      241 gcatcaccct ctccgaaaaa tcggaacgga aacggcgggc gcactatctc aacctgaacg
      301 gatgagtctc tttgcaactg gtggcttggc cgggatagga catcggcagc aagacgcgcc
      361 gatgttatcc atttggccca tcctaatccc ttgatactca acacgatata ccgtaaagtt
      421 cggcatcagt aagatattaa gactttttgc acgctttttg cgtcttaggt gggtgagttg
      481 gtctatcgaa actgaaagaa aggagtaagg cgagatgaag cgattgggac tattggcggc
      541 ggccggggta cttttgttcg gcgggttgtt tcctttggct ttgggcgatg atcgcacaga
      601 acttttgcaa tccagggatg gccagtcaaa ccatgaaccg accagcggcg acgctcttga
      661 cccatcgaag cgtgataccg tacgcgtggc cacggtggac gctttgggcg cgaatgtcgt
      721 cgtcccggtt tatctgttca actcgaaaaa acgcttggcg gggtctctgg tttcgctcaa
      781 atacggcagc gacctggtca cctgcgacag cgtcagtttc gccgacaccc ggctggttgc
      841 cagtgagttt cgggtcgctc aggtgcggct gcaggaacag gtaattcagt tcaccctgtt
      901 gtctgtctcc ggtcacaacg gcgacggtga cgatgaaggt gatgacggaa atgaggacga
      961 agacaatccg ctcacacatt cggtaccacc caaagacttt ggcgtactgg cgttcctcca
     1021 tttccgggtg accggcgcgc cttcaccttc caaccacttt gcgccgatcg acacctttct
     1081 ctcggtaccc ttggtggtac gcttccgcgg agagaagata gatccggtaa agcacaagca
     1141 gaatgcggaa ttgcgccggg ccaccttcgt tcccggaggg atcaatttcc tgacttcgcc
     1201 ggtgacgcgg gaaagggacg gagccctacc gaaatcgttt gagttattcc agaaccaccc
     1261 caatccgttc aatcccagca ccaccctgcg ttttgccgtg ccccagacca gccgggtcga
     1321 actggcggtt tacaacatct taggccagaa gatccggaca cttctggacg aagtggttcc
     1381 cgccggcgtc tactccactg aatggaacgg caccgatgat gccggcatgg gtgcccccag
     1441 cgggatttac tttgcccgct tcgccgccgg cagtcgggtg gcaacgcaaa agatgacgct
     1501 tttgaagtaa gtcagaggca catgcggtca aaaaatcccc cgcctcaggc gggggatttt
     1561 ttgtttacaa aaagcagttg gcagcagagc ggt
//
LOCUS       DBNGZD010000237         4204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1074141, whole genome shotgun sequence.
ACCESSION   DBNGZD010000237 DBNGZD010000000
VERSION     DBNGZD010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1074141"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="AC16GM_06115"
     CDS             <1..702
                     /locus_tag="AC16GM_06115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2923286.1"
                     /translation="SGVIVSTDYQRQRYLNGFGLAPEKVVTIRAGIDFADPGLRSLPR
                     NRMSKNFVIGLVGRFSPIKAHGIFFAAARKIAATLPNAQFIVAGYPAEYTLDDVKRLA
                     QANGVADRCEFFGPVENIYEIISRFDLAVISSTGSEALPRVLLEYLAVGKPVVATAVG
                     AVPEILNQAQCGWLVPPGDALGLARGCLTAYEERERLKTMGARGADFARREFDLDQQV
                     TKTEKFLERLVHGKR"
     gene            689..1528
                     /locus_tag="AC16GM_06120"
     CDS             689..1528
                     /locus_tag="AC16GM_06120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2923287.1"
                     /translation="MESAKSVPATKAMRGHKAGPTISAVVICRNEENVIARCLESLTW
                     ADEIIVVDSDSTDQTLPVAKKFTSRIFTHPWQGFGPQKQFAVEQAHSDWILVVDADEV
                     VTKELAAEIRLVCTGDTLYQGYRIKRKSNFLGRWVLHGGWYPDFVLRLFRTGKGRFTR
                     ARVHESLWVDGSVGTLDGELLHYTDPVLTHYLAKLDRYTGLSAQELFEQHYPFRIWDL
                     LVRPPAVFFKMYVWRAGFLDGIEGFLLALCSAIHVLMKYAKLWEYYHNPNARDLRSGN
                     ASP"
     gene            1555..2574
                     /gene="waaF"
                     /locus_tag="AC16GM_06125"
     CDS             1555..2574
                     /gene="waaF"
                     /locus_tag="AC16GM_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR2923288.1"
                     /translation="MVIRDDRIGDLVLATPVCEALRKKFPESAVTVLASTSAGEILRH
                     NPSVNAVIERSEAAGRIFSKAFWKLVRRVASEHFHLAVILKPSWHTAFLTTFAKIPLR
                     IGTGYRPYSGLFNRRIYVHRKDSTRHELEYNFDVLQPLGITYQGEVPKIFLNEAERER
                     GRTLLEPLRQAESSLVVIHPGGGGGSARFWPVGHFAQLSDRLVEAGYRVVLTGSKADR
                     PVVSEYLGAVRLGPFRLDEKTILRELAAAYAACNLVITNSTGPMHIAAAVGTPVIAFF
                     CPIRTASPRRWGPRGVSHRVLLPLVTECTCAVNRCERGNCMAQITVEQAFASAVSVLT
                     SLSRR"
     gene            2662..>4204
                     /locus_tag="AC16GM_06130"
     CDS             2662..>4204
                     /locus_tag="AC16GM_06130"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR2923289.1"
                     /translation="MNLAEFIIKVESFNANINIPLIRKAYEFSDRAHRGQKRESGDPF
                     IEHCLEVAFILAELHLDSATIAAGLIHDVVEDTEVTIDRVRREFGEEIGLLVDGVTKL
                     SGLKFTSAEEEQVENFRKLLLSMAKDIRVIIIKLADRLHNMRTLEHLPREKISRIAEE
                     THDVYAPLAHRFGMARIRWELEDLAFKYLNAEAYNDIARKINEKRKEREEYIQAVVRP
                     LTDALQRAGIRAEITGRAKHFDSIYRKMLKRDKPFEEIYDLFAIRIVVHTVEECYHVL
                     GVIHSLWTPVTDRFHDYIALPKSNMYQSLHTTVVGPSGKMVEIQIRTEAMHRTAEFGI
                     AAHWLYKEGRHQADAADRQMAWLREVLEWQKELTDPAEFLEYLKIDLFHDDIFVFTPR
                     GEIKQLPRGATPLDFAFAVHTDLGFRCTGARINGRIAPLATPLASGDEVEILASPHRV
                     PHEDWLTIVKTTRARNKIRHWLKQKGYERQVQLGRELLEKECRKKRLAFPSESELNDL
                     ALGMSF"
BASE COUNT          906 a         1184 c         1213 g          901 t
ORIGIN      
        1 tcaggcgtca ttgtttccac tgactaccag cgccagcgtt acctgaatgg gtttggactc
       61 gcaccggaaa aagtagtcac cattcgcgca ggaatcgatt tcgccgaccc gggattacgg
      121 tcactccccc ggaaccggat gagtaagaat tttgtcatcg gattggtcgg tcgtttctct
      181 ccgatcaagg cgcatggaat cttctttgcc gctgctcgga aaatcgctgc gacactgccc
      241 aatgctcaat tcatcgtggc cggctaccct gcggagtaca cgctggatga tgttaagcga
      301 ctggcacagg cgaatggtgt cgcagatcgg tgtgaattct ttgggccggt agagaacatt
      361 tacgagatta tttcgcgatt cgatctcgcc gttatttcct cgaccggctc agaggccctg
      421 ccacgggtgc tcttggagta cctggcggtg gggaagccgg tagtggcgac agcggtgggg
      481 gcggttccgg aaattctcaa tcaggcccaa tgcggctggc tggtcccgcc cggtgatgcc
      541 ttaggcctgg cgcgtggctg cttgaccgcc tatgaagaga gagaacgtct gaagacaatg
      601 ggcgctcggg gagccgattt tgcccggcga gaatttgacc ttgaccagca agtgacgaaa
      661 accgaaaaat tcctggagcg gctcgttcat ggaaagcgct aaatcggttc cagccaccaa
      721 agcaatgcgt gggcataaag ccggtccgac catctccgcg gtagtcatct gccgtaatga
      781 ggaaaatgtg attgcccggt gcctggagag tcttacctgg gccgacgaaa tcatcgtcgt
      841 cgattccgac agcaccgatc agaccctacc ggtggcaaag aaatttacct cccgcatttt
      901 cactcatccg tggcagggct ttggcccgca aaagcagttt gccgttgaac aggcgcacag
      961 cgattggatt ctggtcgttg atgctgacga ggtggtgacg aaagaattag cggcagaaat
     1021 tcgtttggtc tgcaccgggg atactctgta ccagggctac cgcataaagc ggaaatcaaa
     1081 ctttctcgga cgctgggttc tgcacggtgg ctggtacccc gacttcgtgc ttcgcctctt
     1141 tcgcacaggc aagggtcgtt tcacgcgggc gcgggtgcac gagtcgcttt gggtagatgg
     1201 ttccgtcggt acgctcgacg gagaactgct tcactatacc gatccggttc tcactcacta
     1261 cttggcaaaa ttagatcggt acacgggcct ctccgcgcag gagctgtttg aacaacacta
     1321 tccctttcgc atctgggatt tgcttgttcg tccgccggca gtttttttta agatgtacgt
     1381 ctggcgggcg ggctttctgg atggcattga gggattcctt ttggctctgt gctcggctat
     1441 ccacgtcctt atgaagtacg ccaaattgtg ggaatactac cacaatccga acgcgagaga
     1501 cttgcgttcc ggaaacgcct ctccatagtt ttgcagaacg ctccgagaaa aattttggtt
     1561 attcgtgatg accggattgg cgatctcgtc ttagccacac cggtttgcga agcgctgcgg
     1621 aagaaatttc cggagtcagc cgttaccgtc ctcgcgtcaa cttcagccgg agaaattctc
     1681 cgtcacaatc caagtgtcaa tgcggtgatt gagcggtcgg aggcggcggg gcggattttc
     1741 tcgaaagcct tttggaaatt ggtcaggcgg gttgcttccg aacatttcca tctggcggta
     1801 atattgaaac cctcctggca cacggccttt ttgacaacct ttgcaaaaat tccgctccgg
     1861 atcggcaccg gctaccgccc gtacagcgga cttttcaacc ggcggattta cgtccaccgg
     1921 aaagattcaa cccggcatga gctggaatac aattttgatg tactgcaacc attgggtatt
     1981 acttatcaag gagaagtccc caaaatcttt ctcaacgaag cggaacggga acgcgggcgg
     2041 acgttactgg aacccctgcg gcaagccgaa tcgtctttgg tcgtcattca ccccggcggc
     2101 ggtggggggt cggcccgctt ctggccggtg ggccactttg cgcagctttc ggaccgtctg
     2161 gtggaggcgg gataccgggt ggtgctcacg ggctcaaaag cagatcgtcc ggttgtttct
     2221 gagtacctcg gcgcagtacg actggggcca ttccgcttgg acgaaaaaac aattctgcgt
     2281 gaactggcgg ccgcttatgc cgcctgcaac ctggtcatca ccaatagcac cggccccatg
     2341 cacatcgccg ccgctgtggg cacacccgtc attgcctttt tttgcccgat tcgcacggcc
     2401 tccccacgac gctgggggcc gcggggagtg agccaccgtg ttctgctccc cctcgtcacc
     2461 gagtgtacct gtgcggtgaa ccgttgcgag cgcggcaact gcatggcgca gatcacggtc
     2521 gaacaggctt tcgccagcgc ggtttctgtg ctcacctccc tttcccgtcg ataatcttaa
     2581 cagatcacgc tcttaccctc gcaggaggga gggcgtatat tctgtcagag gcacgttgac
     2641 ggaaaaacct aagggccgac tatgaatttg gctgaattca ttatcaaagt tgaatcgttc
     2701 aacgccaaca tcaatatccc actcatccgg aaggcgtacg agttttccga ccgggcacac
     2761 cgggggcaaa agcgggagtc gggcgatcca ttcatcgagc actgcctgga ggtggccttc
     2821 atcctggccg aattgcacct cgactcggcc acgatcgccg ccggtttgat ccacgacgtg
     2881 gtggaagaca ccgaggtgac gattgaccgg gtacggcggg agttcggcga ggaaatcggc
     2941 ttgctggtgg acggggtgac caaattgagc gggctcaagt tcacgagcgc cgaagaagaa
     3001 caggtggaaa atttccgcaa actgctcctc tcgatggcca aggacatccg ggtgatcatc
     3061 atcaaactgg ccgaccgcct gcacaacatg cggacattag agcacctccc cagggagaaa
     3121 atcagccgga tcgccgagga gacccacgac gtttacgcac ccttagccca ccgctttgga
     3181 atggcgcgca tccgctggga gttggaagat ctggccttca aatacctgaa tgcggaggcc
     3241 tacaacgaca tcgctcgtaa gatcaacgag aaaaggaagg agcgggagga gtacatccag
     3301 gcggtggtgc gtccgctcac cgatgctctg cagcgagccg gtatccgggc cgaaatcacc
     3361 gggcgggcca aacacttcga ttccatctat cgcaaaatgc tcaagcggga caagccgttt
     3421 gaagaaatct acgacttatt cgctatccgg atcgtcgttc ataccgtgga ggagtgctac
     3481 cacgttttag gagtgattca ctcgctgtgg accccggtga ccgaccgctt ccacgactac
     3541 atcgctctgc cgaagagcaa catgtaccag tcgctccaca ccacggtagt gggtccctcc
     3601 ggaaaaatgg tggaaattca aatccgcacc gaggcgatgc accgcaccgc cgagttcggc
     3661 atagccgcgc actggctcta caaagaaggg cggcaccagg ccgatgcagc cgaccgccag
     3721 atggcctggc tgcgggaggt gctggaatgg cagaaggaac tgaccgaccc ggctgagttc
     3781 ctggagtatc tcaagatcga cctcttccat gacgacatct ttgtcttcac gccgcgggga
     3841 gaaattaagc agctgccgcg cggggccaca ccattagatt ttgccttcgc cgtccacacc
     3901 gatctgggct tccgctgcac cggcgcgcgg atcaacggcc gcatagctcc cctggccacg
     3961 ccgcttgcct ccggcgacga agtggaaatc cttgcgtctc cccatcgggt tcctcacgaa
     4021 gactggctga ccatcgtcaa aactacgcgg gcccggaaca aaattcggca ctggttgaag
     4081 caaaaggggt atgaacggca ggtgcaactg ggccgggaac ttctggaaaa agaatgccgc
     4141 aagaagcgcc tggctttccc atcagagagc gagttgaacg acctggcgct ggggatgtcg
     4201 ttct
//
LOCUS       DBNGZD010000238         9375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_661182, whole genome shotgun sequence.
ACCESSION   DBNGZD010000238 DBNGZD010000000
VERSION     DBNGZD010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_661182"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="AC16GM_06135"
     CDS             complement(<1..481)
                     /locus_tag="AC16GM_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR2923290.1"
                     /translation="MIKNKTPKKSPARGRRVPTAASLGSSLTHRERITNLIAGKAVDR
                     PPVTFWRHFYHRENAPETLAEALATFQKKFDWDLVKINPRASYHIEDWGAKYRFSKDE
                     FTKPGRLEYPIHVATDWENLEVLTPTRGTLGEQLQAIRLVAKRIGPTVPKVMTVFTPL
                     "
     gene            complement(468..1241)
                     /locus_tag="AC16GM_06140"
     CDS             complement(468..1241)
                     /locus_tag="AC16GM_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR2923291.1"
                     /translation="MTPTALAEQIEKHVRPETFPLAIRMLTVGEAPPEKARRPASDLK
                     AKIAVCQGFGISRRYGWTLAIGEEDISCPLTKVAFGFKKEPAFFTQGFCCEGMYTKTK
                     EAGAKTESQLPKFEYGKYRFVLVGPLARAAYEPHVVLLYANPAQVLRLLTASLFGRGG
                     YHTAKFSGRIDCADEVIRTMLTGEPQVILPCYGDRIFGLVQDHEMAFAFPGSFADELV
                     AGLEGTHKGGIRYPIPQFMRFTGQYPEKYQVLEQYFDKE"
     gene            complement(1241..4345)
                     /locus_tag="AC16GM_06145"
     CDS             complement(1241..4345)
                     /locus_tag="AC16GM_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2923292.1"
                     /translation="MLAKIIAFCLKQKFLILMGVGFLALLGVYAFEHLPIDAFPDVVT
                     PQVQVTSQAKGFSPLEVEKLVTYPIETELAGLPRLAELRSISMPGISSVTAVFEDGVD
                     IYFARQLVLERIISAKERLPGGIQSALSPVTTALGEVYRYTLEYPKEIPRPYGQKELM
                     DLNTAQIWTVKPLLKTVPGVVEVSMLGGYMRQFHVLPRLDRLRKYGVSLKEVFRAVAE
                     NNSVAAGNILDLHSEQNTIRGVGLIKNAQDIETIVVKNHDGTPIFVRDLADVQEGYET
                     RRGAAVENGEGEAVTGLVVMIKDGNAREIVQKIKDKVAEINAAGILPDGMKIVPYYDR
                     TELVDKSTATVEKALLEGILLLVVVLYLFLRNFRSALVVSLALPLAALATFIFMRLLG
                     LSANLMSLGGLAISMGMVIDGAIIQVENVLRHSSENDSQKTHFQLVLDSVLEVQKPSL
                     FGVLIIALTFLPILSLQGMEGKLFSPLAKTVILALLSSLVLSVLVIPVLCLFLIRPQP
                     HKTSGLAEFLKSRYARALDFCLRRKRLVVSAAFLFLALSLAVVPFLGTEFIPHMDEGN
                     VLLQVLRLPSISLEESNKTDLWVQRRLMQFPEVEKIVSNTGTDEAATDVMGQQLSELN
                     ISLKPEKGWKTAKTKEELIEKMRAALAVLPGMAYNFTQQIAMRVDELVTGVKSPVAVK
                     IFGEDFDILSVKAQETARVISAVKGTVDLRVGQQAGQPTLSIEADRRKMARFGINVAD
                     IQEIIEIAIGGKVATEVLEGDRRTEVVVRFPEELRNSVAAIENIPVQSPSGVPVPLGQ
                     LVNFQWEENPAAIEREGGKRRVVIECNAAGRDIGGFVRECREKVKRLVKLPPGYYLTW
                     SGAFENQQRANRRLAVVMPLTVLLVFFLLYTTFGQARQAVLVLLNLPFALTGGVLALL
                     VSGLYLSVPASVGFIALFGVAVLNGVVLISYINQLRQRGTPLEDAIRTGCEKRLRPVS
                     MTALVTMLGLTPLIFATGVGSEVQKPLAVVVVGGLLTSTTLTLLVLPVLYQWFERKKE
                     GRRP"
     gene            complement(4346..5479)
                     /locus_tag="AC16GM_06150"
     CDS             complement(4346..5479)
                     /locus_tag="AC16GM_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2923293.1"
                     /translation="MNVETMRLERNRQMTHLLFALSLSLLLCACGKEKAQDQKERVPA
                     DNPGRVTLTEEGIKLAGIKTEKVAIRSIPVEFSAAGEIGFNEKKLVHLTPRVSGWVDK
                     VYAFVGDRVEAGDSLASLYSPEFLSAQSEFIQAEERLKNVAPADAAEHRTAEALFNSA
                     RSRLLLFGAGEGEISRLAASHQPAPHLVIKSPMSGTVVASNLIPGNTVDKGANLFQIS
                     DLSSLWVTASVYEKDIQGVQKGEQVKVRVTSVGGRTFAGTVQSINDVLDENTRTFKVR
                     IAIENLIGELKPEMFCECFFAGRSGRPILAVPVGAIQQLGTEQIVFVTSGQNSFEKRE
                     VKTGEEVGDYVEILGGVKAGEEVVMAGAFTLKSELFKSGLKEE"
     gene            complement(5476..6702)
                     /locus_tag="AC16GM_06155"
     CDS             complement(5476..6702)
                     /locus_tag="AC16GM_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2923294.1"
                     /translation="MVKVFFTVIALAVGSMAQAQEKPTYSLEGAVAVSLKQNPEVLAA
                     QQQIEAARGRRLQAFSPGQPEFSLKWEKIPAGGSFRQAGDRIFEVGQTLDFPPKLFLR
                     KSAAGRDVDIAFENLGRTQTLVAALVKRAYYKVWLQQKLLASLEFTAGLLRQFQEATL
                     SKYQAGELAYFEVLRANVEAAKIENEISAAKAELASAKTDFNLALGRAGAEDFELADE
                     PAFVPYAKSQDETVRELMKASHTLNIAQLTRERESRNRQLAGWNFVPDLRLTGGAQSG
                     DGGPFRLNVGFGITLPLWWWGPKGQIAETRANLRLAAIRQEALSRTITGEIERSYTLV
                     KAAEGQVALFEKNLLPDVDEELKAGINAYQYNQINALDLLDIYRTAKTAKIEYYRVFL
                     NYLSALADLETAGEEK"
     gene            complement(6864..7706)
                     /locus_tag="AC16GM_06160"
     CDS             complement(6864..7706)
                     /locus_tag="AC16GM_06160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR2923295.1"
                     /translation="MPLREKGLRKVFLFDRRISSLILLTVVKNLSRACLATLLFSYWS
                     LYAQSDSTELEQKSGIKTYAGYCLKDALRFGTAPLWWKGNDWARFGALATGTGILVWR
                     DEEIRKTFQRSRTGTTNHAAKGLNFLGGPGIVPVLVGTLAAGTAFRQKKLQAAAIDGW
                     EASGFAFSCSVVIKTATSRARPFQNEGVWKRERFSTSSSFPSGHTAQAFALATVISSY
                     YHKPWVKILCYGLAASVGLARMNDDKHFASDVLVGAALGTTVARTICKRNQARRASRS
                     KLHR"
     gene            complement(7844..9349)
                     /locus_tag="AC16GM_06165"
     CDS             complement(7844..9349)
                     /locus_tag="AC16GM_06165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR2923296.1"
                     /translation="MAKWMSTVAPRQWWLTASGLFLLRVGIAVWAPLGDDEGYYWVWS
                     KHLALSYFDHPPLVAWLVATSTSVLGVSLFSLRLPFVILGTLTALTLRGLIKKCIGDS
                     RLADTSSLLFQVVPVFFGIGFMVLPDASLLLFWLLTGLLFWQAQQGDKRWAWLGTGAA
                     LGGALLSKYVGVLFVISLAGYLITTQSPRAFKKMSVICALGILLFVPVLWWNAHHHWA
                     SFRYQFVARHHGSGLDGRRFSLFLGSQAIYLSPVLFLLSVCSALRLGPWRLRPEARGE
                     HFFWWLAFPTLGLFALAAAISDFKPNWPAPAYLTLLPLSLLSLARWRQRAPKAAGRIF
                     TTGLVLTLAFTALPTLHLFHPYLPLPQGNDPTADMRGWDEAANQAQRAWDQLRVETAT
                     SGGAPFFAAGRYQLASRLEFYLPAHPSVVCLNLGHDAYDDWQHLSELRGANFIFVASD
                     RFPESPERVAIFDSSKLFARVVARAGPSTTHTITLYQCWRFRPLENPTVSP"
BASE COUNT         2213 a         2678 c         2460 g         2024 t
ORIGIN      
        1 aaagcggcgt gaatacggtc atcacttttg gcacggtcgg cccaattctt ttggcaacca
       61 gacggatcgc ctgtagctgt tctcccagag taccccgcgt gggcgtgagt acctctaagt
      121 tttcccagtc ggtggcaacg tgaatcgggt actccagccg ccccggtttg gtgaattcat
      181 ccttcgaaaa acggtacttc gcgccccagt cttcgatgtg ataggaggcg cgcgggttaa
      241 tcttcaccag atcccagtca aattttttct gaaacgtcgc cagcgcttcg gccagggtct
      301 ccggagcgtt ttccctgtgg taaaaatgcc gccagaaggt gaccggcggg cggtcaactg
      361 ctttgccagc gatgagattg gtaatgcgct cgcggtgggt cagggaagag ccgagagagg
      421 cagcggtggg aacacgacgc ccccgagcag gtgatttctt gggagtctta ttctttatca
      481 aagtactgct ctaagacctg atacttctcc gggtactggc cggtgaagcg catgaactgc
      541 ggaatcgggt agcggatacc gcctttatgc gtgccttcca gcccggccac caactcatcc
      601 gcaaacgatc cgggaaaggc gaaggccatc tcgtgatcct gcaccagacc gaaaatccgg
      661 tcgccgtagc aaggcaaaat tacctgtggt tccccggtga gcatcgtgcg gatcacttca
      721 tccgcgcagt cgatccggcc ggagaacttt gccgtgtggt agccgccgcg cccaaagagc
      781 gacgcggtca aaagccgcaa gacctgggcc ggattggcgt agagcagaac gacgtggggc
      841 tcataggcgg cgcgggccag cggcccgacc aagacgaaac ggtacttccc gtactcgaat
      901 ttgggcaact ggctttcggt tttcgccccc gcttctttgg ttttcgtgta catcccctca
      961 cagcagaaac cctgcgtgaa aaaagccggc tcttttttga agccgaaggc gactttggtc
     1021 aaggggcagg agatgtcctc ttcaccaatg gccaaagtcc agccgtagcg ccgggagatg
     1081 ccgaatccct ggcagacagc gatttttgcc ttcaggtcac tggcgggacg gcgggctttt
     1141 tccggcggcg cttcgccgac tgtcagcatc cggattgcca gcggaaacgt ttcgggccgg
     1201 acgtgctttt cgatttgttc agccaacgct gtgggagtca tcacggtcgc cgaccttctt
     1261 tctttctttc aaaccattga tacaagaccg gcagcacgag taaagttaaa gtggtggaag
     1321 tcaaaaggcc tccgaccacc accactgcca gaggtttctg gacttccgag ccgacacccg
     1381 tggcaaaaat taggggtgtc agcccgagca tggtcaccag agcagtcatt gaaaccggcc
     1441 gcaaccgttt ttcacaaccg gttctaatcg cgtcctccaa aggcgtacct cgctggcgca
     1501 gttggttaat ataggaaatt aacacgacgc cgttcaagac cgccactccg aaaagggcga
     1561 taaagccgac cgacgctgga acggaaagat agaggccgga gaccaagagg gccaagaccc
     1621 cgccggttaa ggcgaatggg agatttaaaa gcacgagcac cgcctgccgg gcctgtccga
     1681 aggtggtata gagaagaaag aagaccaata aaactgtcaa aggcattact actgccaggc
     1741 ggcggttggc cctttgctga ttttcaaaag ctccgctcca ggtgaggtag tacccgggcg
     1801 gcagcttgac cagcctttta actttttcgc ggcactcccg cacgaagccg ccgatgtccc
     1861 gcccggcggc attgcactcg atgaccaccc ggcgcttgcc gccctcgcgt tcaatggcgg
     1921 ccggattttc ttcccattgg aaattcacca gttggcccag cggaaccggc accccggagg
     1981 gggactggac cggaatattt tcaattgccg ccaccgaatt gcgcaactct tcgggaaaac
     2041 gaaccacgac ttccgtgcgg cggtcccctt ccaaaacttc ggtggccacc ttgccgccaa
     2101 tggcaatttc aatgatttcc tggatatccg ccacattgat gccgaaacgg gccatcttcc
     2161 gccgatcggc ttcaatcgag agggtaggct gtccggcttg ctgtcccacc cgcaaatcga
     2221 ctgtcccctt gacggcagaa ataacccgtg cggtttcctg cgctttgacc gagaggatgt
     2281 caaaatcctc accgaagatt ttaaccgcca ccggtgattt cacccccgtg accagctcat
     2341 ccacccgcat cgctatctgc tgggtgaaat tgtaggccat gccgggaagc accgccagtg
     2401 ccgcccgcat tttttcgatc aactcctctt tggttttggc ggttttccat cctttttcag
     2461 gtttcaaaga aatgttgagc tcgctgagct gctggcccat cacatcggtg gcggcctcat
     2521 ccgtgccggt attggaaacg atcttttcca cctcgggaaa ctgcatcaag cgcctctgca
     2581 cccacaggtc ggttttattc gattcttcca gcgatatgct gggaagccgc aaaacttgga
     2641 gcaaaacgtt gccttcatcc atatgaggga tgaactcggt ccccaaaaag gggactaccg
     2701 ccagagaaag agccaaaaac aaaaaggcag cggacaccac caagcgctta cggcgcaggc
     2761 aaaaatccaa agcccgagcg tagcggcttt ttaaaaactc cgctaggccg gaggttttat
     2821 gaggctgggg gcggatgagg aaaaggcaaa gcaccgggat gaccaaaacc gaaagcacca
     2881 gcgaggaaag cagggccaag atcactgtct ttgccagggg ggagaaaagt tttccttcca
     2941 ttccctggag cgatagaatc ggcaaaaaag tcaaagcgat aatcaaaact ccgaataagc
     3001 tcggtttttg tacttccagg accgagtcca aaaccagctg aaaatgggtt ttctgagagt
     3061 cgttttcact cgaatgccgc aaaacgtttt cgacctgaat aatggctcca tcaataacca
     3121 ttcccatgga aatcgccagc ccgcccagag acattaaatt ggccgaaagt cccaaaagac
     3181 gcataaaaat aaaggtggct aaagccgcca ggggcaaagc caaagagacc actaaagcgc
     3241 tgcggaagtt gcgtaggaac agatagagaa cgaccaccaa caacaaaatt ccttccagga
     3301 gtgccttttc caccgtggcg gtgctcttat ccaccagctc cgtgcggtcg taatacggca
     3361 cgattttcat tccgtccggc aggatgccgg cggcgttaat ttctgccact ttgtctttta
     3421 ttttttggac gatctcgcgg gcattgccgt ccttgatcat caccaccaag ccggtgactg
     3481 cctccccttc cccattttca actgccgccc cccgccgggt ttcgtaccct tcctgcacgt
     3541 ccgccagatc ccgcacaaaa atcggcgtcc cgtcgtggtt tttaaccacg atagtttcga
     3601 tgtcttgggc gttcttgatt aatccgacgc cgcggatggt attctgttcg gaatggaggt
     3661 ccaatatatt tccagccgcc accgagttgt tttctgcgac cgctcgaaaa acttctttta
     3721 acgaaacacc atatttgcgc aggcggtcca gccggggaag aacgtggaac tggcgcatgt
     3781 agccgcccaa catgctcacc tccaccactc ccggcacggt ttttaaaagc ggtttgaccg
     3841 tccaaatctg cgccgtgttc aaatccatca gctctttttg gccatacggg cgggggattt
     3901 ctttcggata ttcgagcgtg taacggtaca cttcccccaa ggcggtggta accggggaaa
     3961 gcgcgctttg aatcccgccc ggcagccgtt ctttggccga aataattcgc tccagaacca
     4021 gctggcgggc aaaataaatg tcaactccgt cttcaaaaac ggccgtcacc gaagagatac
     4081 cgggcatgga gatggagcga agttctgcca gccgaggcag gccggccaat tcggtttcaa
     4141 tcggataggt gaccagtttt tccacttcga gcggagaaaa ccccttggcc tggctcgtga
     4201 cctgcacctg tggagtgacg acgtccggaa aagcatcgat gggaagatgc tcgaaagcat
     4261 aaacacccag gagcgcaagg aatccgaccc ccattaaaat gagaaacttt tgcttcaggc
     4321 aaaatgcaat gattttagcc agcatttatt cttctttcag ccccgatttg aaaagctccg
     4381 atttcagagt aaaagcccct gccatcacca cttcctctcc ggctttcacc cctcccagga
     4441 tttccacata gtcgcccact tcttcgccgg ttttgacctc ccttttttca aaggagtttt
     4501 gacccgaggt cacgaaaaca atctgctccg ttcccaactg ttgaatggca ccgacgggga
     4561 cggccagaat gggccgcccg ctccgaccgg caaaaaagca ttcgcaaaac atttcgggtt
     4621 tcagttctcc aatcagattt tcaatggcaa ttcggacttt aaaagttcga gtgttctcat
     4681 ccaagacgtc gttgattgac tgcaccgttc cggcaaaagt ccggccgccg accgaggtaa
     4741 cccgaacttt cacctgctcg cctttttgca cgccctgaat gtctttttcg tacacgctgg
     4801 cggtgaccca aagggagctc aagtccgaga tttgaaacag gttggccccc ttatcgacgg
     4861 tgtttcccgg aatcagattg ctggccacta ccgttccact catgggactt ttgatgacca
     4921 gatggggagc cggctggtgg cttgccgcca gccgggatat ttctccttcc ccggcgccga
     4981 aaagcaaaag gcgggagcgg gccgagttga acaacgcctc ggccgtgcgg tgctccgccg
     5041 catcggccgg cgccacattt tttaaccgct cttcggcctg gataaattcg ctctgggcgg
     5101 agagaaactc cgggctgtac agggaagcca ggctgtcgcc agcttcgacc cggtcgccga
     5161 caaaggcgta caccttatcc acccagccgg aaacccgcgg cgttaggtgc accagttttt
     5221 tttcgttgaa accgatttca ccggcggcgg agaactccac cggaatgctg cggatcgcta
     5281 ccttttcggt tttgatgccg gccagcttga ttccttcttc ggtcaaggtc acgcggccgg
     5341 gattgtccgc tggcaccctt tctttctggt cctgcgcctt ctctttgccg caagcacaaa
     5401 gcaggaggga caaagagagc gcaaaaagta aatgggtcat ttgacggttg cgctcgaggc
     5461 gcatagtctc aacgttcatt tttcctcccc cgccgtttcc aaatcggcca gcgcggacag
     5521 gtaattcaaa aacactcggt agtactcgat tttggcggtt ttcgcggttc ggtagatgtc
     5581 caaaagatcc aaagcgttta tttgattgta ctgataggcg ttgatgccgg ctttgagctc
     5641 ttcgtccacg tcgggcagca ggtttttttc aaagagcgcc acctgtccct cggctgcttt
     5701 caccagcgtg taacttcgct cgatttcgcc ggtaattgtt cgcgataatg cctcttgccg
     5761 gattgcggca agccggagat tggcccgggt ttccgcgatt tgccctttgg gcccccacca
     5821 ccataaaggc agcgttatac caaaacctac gttgagtcgg aaaggcccac cgtctccact
     5881 ttgtgccccg ccggtcaacc tcaggtcggg cacaaaattc caacctgcca actgccggtt
     5941 tcggctttcc cgttccctgg tcaactgggc gatgttcagg gtgtggctgg ctttcatcaa
     6001 ttcccgcacg gtttcgtctt ggctttttgc gtagggcacg aacgccggct cgtccgccaa
     6061 ctcaaaatct tccgcaccag cccgtcccag cgcgaggtta aaatcggttt tggccgaggc
     6121 caattcggct ttcgccgcgc tgatctcgtt ttcaatcttg gccgcctcca cgttggcccg
     6181 gaggacttca aaataggcca gttccccggc ctggtatttc gacaacgtcg cttcctgaaa
     6241 ctgccgtaac agcccggcgg taaactccag cgacgccagg agtttttgct ggagccaaac
     6301 tttgtaatag gcccttttta ccagggccgc aaccagcgtc tgggtgcggc ctaaattttc
     6361 gaacgcgatg tcaacgtccc gcccggcggc gcttttcctc agaaacagct tgggtggaaa
     6421 atccagggtt tgcccaactt caaaaatccg gtccccggcc tgacgaaagg aaccgccagc
     6481 aggaattttt tcccatttga gcgagaattc cggctggccg ggcgaaaaag cctgcaacct
     6541 gcggccccgg gccgcctcaa tttgctgttg ggctgccagc acttcgggat tctgttttaa
     6601 ggaaacagcc acagctccct caagagaata ggtcggtttc tcctgagctt gtgccattga
     6661 accgacggca agtgcaatca ccgtaaaaaa aactttgacc atttcactgc ttgataagtt
     6721 tcaatcgttg ttctcctttt cagacatacg cgaaaaccgg gaaattgttt ttcgcagaaa
     6781 agttgtgacg cctgaaccga ataaacaaac cccatcaagt gttgttaatt ccaacttcct
     6841 caattgaaaa aaacgattgc tcttcaacgg tgtagcttgg atcgggaagc acgccgggcc
     6901 tggtttcgtt tacatatggt gcgggccact gttgttccca atgccgctcc cacgagaaca
     6961 tcggaggcaa aatgtttgtc gtcattcatt cgagccaacc caacgctggc agccaacccg
     7021 tagcagagaa ttttcaccca gggcttgtgg taataactgc tgatcactgt cgccagcgcg
     7081 aaggcttgag ctgtgtggcc ggagggaaag gaggaactgg ttgaaaatct ctcccgcttc
     7141 cacacccctt cgttttgaaa cggtcgagcc cggctggttg ccgttttgat aaccacggaa
     7201 caagaaaagg caaagccgga ggcctcccag ccatcgatcg cggccgcctg caattttttc
     7261 tgtctgaaag ccgtaccggc tgcgagagtt ccaacaagca ccgggacaat tccaggtcct
     7321 ccaaggaagt tcagcccctt ggcggcgtga tttgttgtac cggtgcgact gcgctgaaat
     7381 gtttttcgta tctcttcatc gcgccagacg agaatcccgg tgccggtagc cagagcgccg
     7441 aagcgagccc aatcgttgcc tttccaccac agcggtgcgg taccgaatcg taaagcatcc
     7501 ttcaagcagt atcccgcata ggttttgatg ccggattttt gttctaactc agtggaatcg
     7561 gattgggcgt aaagagacca atagctgaac aggagagtcg ccaggcaagc tcggctgaga
     7621 tttttgacca ctgttagcaa aataagcgaa gagattcgac ggtcaaagag aaaaaccttt
     7681 cgcagcccct tttcccgcaa gggcaataac tggtgtcacc gaccgcatga gtttttcaat
     7741 ttctttaggt ctatgcgaag tggcactgct ttgtccgccc cgtatcgatg tacgacgagc
     7801 tctggacagg atttggctgg cccacagaac cgccgcgcat tctctaaggt gaaacagtcg
     7861 gattctccag gggccggaat ctccagcatt ggtagagggt aatggtgtgc gtggtggagg
     7921 gacctgctct cgctaccact cgggcaaata gcttagagga atcgaagatt gctacccgct
     7981 ccggtgattc cgggaaacgg tcgctggcca cgaatatgaa gttcgctcca cgcagttcac
     8041 tcaaatgctg ccagtcgtca taggcgtcgt ggcccaagtt aagacaaacg accgatgggt
     8101 gggcagggag atagaattca agtcgggatg caagctggta acgacctgct gcaaagaaag
     8161 gcgcaccacc ggaggtagcc gtctctactc gtagttggtc ccaggctcgt tgggcttggt
     8221 tcgcagcttc gtcccaaccc cgcatgtcag ctgtcggatc attcccttga ggtaggggca
     8281 aataagggtg gaaaagatgg agggtgggca gggccgtgaa ggccagagta agtaccagcc
     8341 ccgtcgtgaa tattcgccct gccgccttag gggcccgctg gcgccaacgt gccaggctca
     8401 aaagcgaaag gggaagcagt gttaagtagg ctggtgccgg ccagttaggt ttaaaatcag
     8461 aaatggcagc cgccagcgca aagaggccaa gagtgggaaa agccagccac cagaaaaagt
     8521 gctcaccccg tgcctccggg cgtaaccgcc aaggccctaa ccgcagggcg gagcacaccg
     8581 aaagcagaaa tagaaccggt gagagataaa ttgcctgtga ccccaaaaaa agagaaaatc
     8641 tccggccgtc caaacctgac ccgtgatgac gggcaacaaa ctggtaacga aacgatgccc
     8701 aatggtgatg ggcgttccac caaagaacag gcacaaaaag caaaatccct aaagcacaaa
     8761 tcaccgacat ttttttgaac gcccgcggac tctgggtagt tatcagatat ccagccaatg
     8821 aaataacgaa aagtactccc acatacttgc tcaaaagcgc accgccgagt gctgctcccg
     8881 tcccaagcca ggcccacctc ttatcccctt gctgcgcctg ccagaacaaa agcccggtga
     8941 gaagccaaaa caaaagcagg gaagcatccg gcaaaaccat aaaccctatg ccaaagaaaa
     9001 ccggcaccac ctgaaagagc aatgagctag tatccgctaa tcgggagtca ccgatgcact
     9061 tcttgattag cccacgcagg gtcaatgcgg tcagagtgcc taaaatgaca aatggcaacc
     9121 tcaacgagaa gagagaaaca ccaagaacgg aagtggaggt ggccaccaac caggctacta
     9181 agggagggtg gtcgaaatag cttaaggcca gatgttttga ccagacccag taatacccct
     9241 catcatcccc aagaggcgcc caaaccgcaa tccccacgcg gagaagaaac agcccagacg
     9301 cggtcaacca ccattgccgg ggcgccaccg ttgacatcca ttttgccatt gcccgacttc
     9361 aacaacccgg tcacc
//
LOCUS       DBNGZD010000239         5832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1912507, whole genome shotgun sequence.
ACCESSION   DBNGZD010000239 DBNGZD010000000
VERSION     DBNGZD010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1912507"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="AC16GM_06170"
     CDS             complement(<1..491)
                     /locus_tag="AC16GM_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HIR2923297.1"
                     /translation="MSIRVAINGFGRIGRLVFRAGYRNPDLEFVAVNDITDAPTLAHL
                     LKYDSVHRRMEADVRAEGSAIVVNGHRTEVLAEKEPANLPWKKLGIDVVVESTGKFTD
                     REAAVKHISAGAKKVLISAPAKKPDGMIVLGVNHRIYDPAKHQIVSIGSCTTNCLAPV
                     AKVL"
     gene            complement(516..1172)
                     /locus_tag="AC16GM_06175"
     CDS             complement(516..1172)
                     /locus_tag="AC16GM_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923298.1"
                     /translation="MGVIFKKILPGLILLSSVLTGTVAPKEFSQAHQFGGRFGILRIK
                     DAALGLNFPGFEIQKDGTLPYIEFFYQPRWNRYLSLDLSFGAVGRGEVRYIDQVNRLY
                     FFSSVNVYPISVGVNLQPLAFGHQPRFFPVVRGGGTFAIATESDLFGVGSKTQSAFGV
                     YLAPGLDVALGSFFFTAEAGYRTARFRDPVGGIKDYSGVFIGLGVGYSYSGRVPGKHH
                     "
     gene            complement(1300..2031)
                     /locus_tag="AC16GM_06180"
     CDS             complement(1300..2031)
                     /locus_tag="AC16GM_06180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR2923299.1"
                     /translation="MTFTMPIQAVTFDLWETLVAHTPENDVAIRQLRYERMQTALGRE
                     IHREQFQTAYRRSWEKFEEIWITNREVQTEEQLDIFLTLLFNSSRPALNAETRKKLED
                     AYLSPVRDHPPVVYPDVLPVLTELQKRKLPLGMICNTGRTPGRLLRPILQAQGILDFF
                     RVALFSNELDLRKPAPEIFHVAIRELGAVPETTVHVGDRPNLDIQGALGAGLQAVQID
                     RYELYQPTGKWPFIRSLPELIPLLD"
     gene            complement(2028..4562)
                     /locus_tag="AC16GM_06185"
     CDS             complement(2028..4562)
                     /locus_tag="AC16GM_06185"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR2923300.1"
                     /translation="MGKKFTPKAFFILIFATLLWGGFAVYATFAVKTLPTSAQDGVFW
                     EKVPGGLVPREILPGSPADRAGLLPTDTLLLVNRVSVTSEKAVQEVIEQLPLARPAQY
                     TVRRDGALVKLEVAVDRKTFEVVRRWLLQLAGFGYIILALFVYRKKPELPTARAFFFF
                     GLLAGSTLTAFFVDASRLPDFLQPVFLLWIQLNIFFIPALGLDFFRRLVFFPPARVPP
                     RGLTLLTYLPAFLGFGLMALALSVAKGWLTIEPVIGFLGKIRPLFQPGNLEPANWGIY
                     LLWFVLEFAVRVKKKEVVINARQRQWLQWGVVRPLLVYVVFNQVLPLFWSWGLLKWSA
                     LVILPSQAVLAYLILKERLLEIGVVIKRSAIYAILSASLIGIFVLLVLGISQLMVLLT
                     GQTSILAAFAAALLTAIVLNLYRQKFQNYLDRKFFKDRYNYQRALLEFSKELGRLENL
                     ETLLAKISKQFAEALHLSNCLPFLYNQKADAYVMVAPYGLAEPVLSQVRYAASQFGLA
                     TLLVQEKHPLDFYDLETKPLYFYLPVVEKVALKKTDTALAVPLLVKEKLVGMLLLGNK
                     KSGDLFSSEDVDFFATLSAPLAVAVENARLHQEELGKKELEREIVLAGEVQKRFLKTV
                     PAQLPGLEIGALNLPSRQVSGDLYAIIPLDEKRLGIAIGDVAGKGMSASLLMALVQAT
                     LETLAPSVAAPAELLQRLNTAVCRNSTPGRFVTLFYGIYDTETKRFAYANAGHNPPVW
                     LGRQGVRALDEAGLLLGVDPKARYPQGLVHLKPEDLVVLYTDGVTEASNTDEHRFEEE
                     RLHQCLLKSRTLSAPDVLQAVVEAVRAFAGTSEFEDDLTLVGLKVV"
     gene            complement(4661..>5832)
                     /locus_tag="AC16GM_06190"
     CDS             complement(4661..>5832)
                     /locus_tag="AC16GM_06190"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR2923301.1"
                     /translation="VSKQFAEALHLYTCLPFIYDQKADAYVMVAPYGLSDPALQQVCY
                     PASRFGLATLLAQERRSLEFYALETDPLYTYLPVVEKVALKKTDTALAVPLLVKEKLV
                     GMLHLGNKKSGDQFNSEDIDFFATLSAPLAVAVENARLYQQEVEKREMERELVVARQI
                     QQKLVTRELPKMPGVDLAAAYLPSRQVSGDIYCILPVRENQIGLAVGDVSGKGVPASL
                     LMATVRASLITLVEEGLPPPRMMSRLNRLVYENTEPEHFVTFFYAVYDRVTRKLSYVN
                     AGHNPPLYFPADGQPQELSTGGLILGVLPDGHYQAGDVIVQPNSLAVFYTDGVTEAAN
                     ESEAQFGTPRLIETIRHHRFLPADTLCGKILAAVREFCGDRELGDDVSLVILKTE"
BASE COUNT         1333 a         1681 c         1663 g         1155 t
ORIGIN      
        1 agcactttgg ccaccggcgc caggcagttg gtcgtacaag agccgatgga gacgatctgg
       61 tgtttggccg gatcataaat ccggtggttg acgcccaaga cgatcatccc gtccggcttt
      121 ttggcggggg cggaaatcaa aactttcttc gctcccgctg aaatatgttt caccgccgcc
      181 tcccgatcgg tgaacttgcc agtcgattcc accaccacgt caattcccaa ctttttccag
      241 ggcaaattag ccggttcttt ttcagccagt acttcggtgc ggtggccgtt gacgacaatc
      301 gcactgccct cggcgcgcac gtcggcttcc atgcgccgat gcaccgaatc gtacttcaaa
      361 aggtgcgcta aggtcggcgc gtcggtgatg tcgttgaccg cgacaaactc caggtcggga
      421 ttgcggtatc cggcgcgaaa gactaaacgc ccgatccgcc cgaacccgtt aatggctact
      481 cgaatcgaca tgtctcctcc acaattttcg atgcttcagt ggtgcttccc tggtacgcga
      541 cctgagtacg aataccctac gcccagtccg atgaaaaccc cgctgtaatc cttgatcccg
      601 ccgaccgggt cacgaaatct ggccgtccgg tagccggctt cagccgtaaa gaaaaatgag
      661 cccagcgcca catccaaacc gggtgccaag tagacgccga aggccgattg ggtcttcgac
      721 cctacgccaa aaagatcgct ctcggtggcg atggcgaagg tcccgccccc gcgcacgacc
      781 ggaaagaagc ggggctggtg cccgaacgcg agcggctgaa ggtttacccc gactgaaatc
      841 gggtagacat tgacggaact aaaaaaatag agccgattga cttgatcaat gtagcggact
      901 tcacctcggc caacggcgcc gaaagacaga tccaacgaga ggtagcggtt ccagcgcggc
      961 tggtagaaga attcgatata cggcagggtg ccgtctttct gaatttcaaa ccccggaaaa
     1021 ttgagcccca acgccgcgtc cttgatccgc aggatgccga accgcccgcc gaactggtgc
     1081 gcctgcgaga attctttcgg cgcaaccgtc cccgtgagaa cgctgctgag caggatcaac
     1141 ccgggtaaga tttttttgaa gattactccc atacgaaact ccgactgttc acccttcaag
     1201 gatacgacac cgatgctaat ttctctatgc cggaaagtca agacgacggt cgaaaggaaa
     1261 ttcgagaatt cataaattgc ttctctcttc aagggggaat tagtccagaa gcgggatcag
     1321 ttccggcaaa ctgcggatga agggccattt gcccgtaggt tgatagagtt cgtagcggtc
     1381 aatctggacg gcctgaagtc cggcacccaa agcaccctgg atgtcgagat ttggccggtc
     1441 gcccacgtgc acggtcgttt ccggcacagc accgagttcc ctgattgcaa cgtggaagat
     1501 ctccggagcc ggcttgcgca ggtcgagttc gttggaaaaa agagccacgc gaaagaagtc
     1561 gaggataccc tgcgcttgca aaatcggccg caatagccgc cccggcgtcc ggccggtatt
     1621 gcagatcatc cccaggggca acttccgctt ttgcagttcc gttagaaccg gcagcacgtc
     1681 ggggtagacc accggcgggt ggtcgcggac cggagacagg taggcatctt cgagtttctt
     1741 tcgagtttca gcattcagcg cggggcgaga actgttaaag agcagtgtca ggaaaatgtc
     1801 cagttgctcc tcggtttgca cttcccgatt ggtgatccag atttcctcaa atttttccca
     1861 cgagcggcgg taggccgttt gaaactgttc ccggtgaatc tcccggccca gtgccgtctg
     1921 catccgctcg tagcgcaact ggcggatggc cacgtcgttc tccggggtgt gggcaaccag
     1981 cgtttcccag agatcaaagg tgacggcttg aatcggcatg gtgaaagtca caccaccttg
     2041 aggccgacca aggtcaggtc atcctcaaat tcgctggtcc cggcaaaggc acgtaccgct
     2101 tccaccaccg cttgcagcac atcaggggcg gagagcgtcc ggcttttgag caggcactgg
     2161 tgcagccgtt cttcttcgaa ccggtgctcg tcggtattgc tcgcttcggt aacgccatcg
     2221 gtgtagagca ccaccaggtc ttctggcttg aggtgcacca gcccttgcgg gtagcgggcc
     2281 ttgggatcga cccccaggag caaaccggct tcatccagcg cccgaacgcc ctgccggccc
     2341 agccagaccg gcggattgtg tcccgcattg gcgtaggcga agcgtttggt ctccgtatcg
     2401 taaatgccgt agaagagagt cacgaagcgg cccggagtgc tgttgcggca gacggcggtg
     2461 ttcaagcgct gcaaaagctc cgcgggcgca gcgaccgaag gcgccaaagt ctccagagtc
     2521 gcctgcacca gggccatcaa gagcgaagcg ctcatccctt tgccggccac gtcccctatg
     2581 gctatgccca gccgcttctc atcgagcgga atgatggcgt agagatcgcc ggacacttgc
     2641 cgggaaggca aattcaaagc gccgatttcc agccccggta gctgcgcagg gacagtttta
     2701 aggaaccgct tctgcacctc tccggctaag acgatctcac gttccagttc tttttttccc
     2761 agttcttctt gatgcaagcg ggcgttttcc accgccaccg ccagaggcgc cgagagggtc
     2821 gcaaagaaat cgacgtcctc agagctgaac aagtcgcccg actttttgtt ccccaggagc
     2881 agcatcccga ccagtttctc tttgaccagg agcggcaccg ccagtgcggt gtcggtcttt
     2941 ttcaaagcca ctttttccac caccggcagg taaaagtaga gtggcttggt ctccagatca
     3001 taaaaatcca gcgggtgttt ttcctgcacc agaagagtcg ccagcccgaa ctgggaggcc
     3061 gcgtagcgca cctgggagag caccggctcg gccaggccgt acggcgccac catgacgtag
     3121 gcgtcggcct tctggttgta gaggaaaggc agacagttgg acaaatgcaa tgcctcggca
     3181 aactgtttgg aaattttggc cagcagcgtt tccaagttct ccaggcgacc cagctctttg
     3241 gagaattcca gcaaggcccg ctggtagttg taccggtctt tgaagaactt gcgatccagg
     3301 taattctgaa acttctgccg gtagagattc aggacgatgg cggttaaaag ggcggcggcg
     3361 aaagcggcca aaatcgaggt ctggccggtc aagagcacca tgagctgact gatgccgagc
     3421 accaacagaa cgaagatacc gatgagactg gcggaaagaa tggcgtagat ggcggagcgc
     3481 ttgatcacca ctccgatttc caagagccgc tctttcagga tgagatacgc cagcaccgcc
     3541 tgcgagggga ggataaccag agcgctccat ttcaaaagtc cccacgacca gaagagcggc
     3601 aggacctggt tgaagacgac atacacgagc aacgggcgaa ccacacccca ctgcagccac
     3661 tgcctttgcc gggcattgat cactacttct ttctttttta cccggacggc gaattccagc
     3721 acaaaccaga gcaaatagat accccagttc gccggctcca ggttaccggg ttgaaacagc
     3781 ggtcttattt ttcccaaaaa gccgatgacc ggctcaatcg tcagccaccc tttggcgacg
     3841 gagagtgcca gtgccatcag gccgaatccc agaaaagcgg gcaggtaggt caaaagggta
     3901 agtccccgtg gaggcactct ggcgggcgga aagaacacca gccgccggaa gaaatccagt
     3961 cccagcgcgg ggataaaaaa gatgttcagt tgaatccaaa gcaaaaagac cggctggagg
     4021 aaatcgggca aacgggacgc atccacgaag aacgcggtca aagtcgagcc ggccaaaagg
     4081 ccgaaaaaga aaaaggcccg ggcggtaggg agctccggtt ttttgcggta aacgaaaagg
     4141 gctaagatga tgtacccaaa accggccaac tgcaaaagcc agcgccggac tacctcaaaa
     4201 gtcttccggt ctaccgccac ttccagtttg accaacgcac cgtcgcggcg gacggtgtat
     4261 tgcgccggcc gggcgagcgg cagttgctcg atgacctcct gcaccgcctt ctccgaggtg
     4321 acgctgaccc ggttgaccag aaggagggtg tcggtcggca gaagaccggc gcggtcggcc
     4381 ggactcccgg gcagaatctc ccggggcacg aggccgccgg gaactttttc ccagaagacg
     4441 ccgtcctgag ccgaagtcgg cagggtcttc acggcaaagg tggcatagac ggcgaaaccg
     4501 ccccagagca gagtcgcaaa aatgaggata aagaaagctt taggagtgaa ctttttgccc
     4561 acgatgggaa attactaaac tttcaaaaca atcaaaatgc tatcgtcccg gcttttgtga
     4621 ttctggaaaa acgcttcttc tcaattgttt tgggttgcat ttattctgtt ttcaatatga
     4681 ctagggaaac gtcatctccc aactctcgat caccgcaaaa ttcccggacg gcggctaaaa
     4741 tttttccgca cagcgtatct gcggggagga accgatggtg cctgatggtt tcgatcagcc
     4801 gcggggtgcc gaattgcgct tcggattcgt tagcggcttc agtcacgccg tccgtataga
     4861 aaacagccag cgaattgggc tggacaatta cgtctccggc ttggtagtga ccgtccggca
     4921 gcacgcccag aatgagccct ccggtcgaaa gctcctgggg ttgaccgtcg gcgggaaaat
     4981 agaggggcgg gttgtgaccg gcgttcacat agctcaattt tctggtgacc cggtcgtaaa
     5041 cggcataaaa aaacgtcacg aaatgctccg gctcggtatt ctcgtacacc agccggttca
     5101 gacgggacat catccggggt ggcggcaggc cctcctccac cagcgttatg agagaagcgc
     5161 gtaccgtggc catcaaaagc gaagccggca cccccttacc ggagacgtcg cccacggcca
     5221 agccaatctg attttctcgc accggcagga tacagtaaat atcgccggaa acctgccggg
     5281 aggggaggta ggccgccgcc agatcgactc ccggcatttt cggcagttcg cgggtcacca
     5341 gtttctgctg tatctggcgt gcgaccacca gctcccgttc catctcgcgc ttttccactt
     5401 cctgttggta gagccgggcg ttttccaccg ccaccgccaa aggcgccgaa agggtggcaa
     5461 agaaatcaat gtcctcggag ttgaactggt cacccgattt cttgttgccg aggtggagca
     5521 tcccgactaa tttctctttg accagaagcg gtaccgccaa ggcagtatcg gtcttcttca
     5581 aagccacctt ttcgaccacc ggaaggtagg tatagagcgg atcggtttcc agcgcgtaaa
     5641 attccagcga ccgtctttcc tgagccaaga gcgttgccag cccaaaccgg gaagccgggt
     5701 agcacacctg ttgcagggct gggtcggaga gcccatacgg agccaccatc acgtaggcat
     5761 ccgccttctg gtcgtaaatg aacggcaggc aggtgtacag atggagtgcc tcggcaaact
     5821 gcttggatac tt
//
LOCUS       DBNGZD010000240        11512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1496079, whole genome shotgun sequence.
ACCESSION   DBNGZD010000240 DBNGZD010000000
VERSION     DBNGZD010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11512
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1496079"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /gene="folP"
                     /locus_tag="AC16GM_06195"
     CDS             <1..925
                     /gene="folP"
                     /locus_tag="AC16GM_06195"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR2923302.1"
                     /translation="IKDHFTPIDYSVRMKVSPENAAPKKKSKRGVIRCGEQEINLFSR
                     THIMGVLNVTPDSFYDGGRYYDLRRAVNRAREMTDEGADFIDIGGLSTRPGSEPVSEE
                     EEIKRVIPVVTEVATQVSIPLSIDTYRASVARAALDAGASLVNDISGLAFDPAMPKLV
                     AESGCPVVIMHIKGTPRDMQVDPHYDDVIAEISRHFRDRIASAKSAGIADEQILLDPG
                     IGFGKRMEDNLVILQRLRDFADLGYPLLVGASRKSFIGKILDLPSAERLEGSLAAAAV
                     AIQNGAAIIRTHDVKATVRVARIVDAIRRSG"
     gene            962..1726
                     /gene="cdaA"
                     /locus_tag="AC16GM_06200"
     CDS             962..1726
                     /gene="cdaA"
                     /locus_tag="AC16GM_06200"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR2923303.1"
                     /translation="MNFFTFGFLKFGLVDAVDIGLVSFVTYKLLSLMKGTRSAQILTG
                     LFLVIAVSFLAFWFQLEAVKWLITNIATVGLLVLVVLFQPEIRGALAQIGHNRVVRLF
                     YKYEEQKSLDEIVRATQKLAESKTGSLIVLERQVGLKSYIESGRLMHAQVSEELLITI
                     FSPRAPLHDGAVIIRGDLVVAAGCTLPLSRTLEFTAPHGMRHKAATGITEESDAIAVA
                     VSEETGEISVAGWGRLERGIDPHALRDHLVRLLRSQ"
     gene            1793..2776
                     /gene="obgE"
                     /locus_tag="AC16GM_06205"
     CDS             1793..2776
                     /gene="obgE"
                     /locus_tag="AC16GM_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR2923304.1"
                     /translation="MFVDYVEITVKAGDGGHGCLSFRREKYVPKGGPDGGDGGRGGHV
                     IARVDPHLATLLDLRYQRLYRAAKGGDGQGKDLFGREGEDRVIPVPPGTTIKDLNTGK
                     VIADLTLPDQEVLLARGGRGGHGNAFFKSSTNRAPRRFEAGKKGEEKRLTFELKLIAD
                     VGLVGFPNVGKSTLLAKVSRARPKIAPYPFTTLTPHLGIVRVGDWRSFVLADIPGIIA
                     GAHQGKGLGLEFLRHIQRTRVLVYLLDATSSTLRRDYQILKAEVKAYDPEITRKPSLV
                     VLNKIDLLNGQPKKTLRVSMPVFYISALTGQGIKELLFGLEEVLGSVSRAA"
     gene            2779..3909
                     /gene="dprA"
                     /locus_tag="AC16GM_06210"
     CDS             2779..3909
                     /gene="dprA"
                     /locus_tag="AC16GM_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR2923305.1"
                     /translation="MALTDAEKENWLALSLVPNVGPLHFTALVKRFGSAGAVLEAGEK
                     SLLEVPGVGLKTAAAIRRTPVREEARKQLALLQKEKCDLITLADESYPFNLKNISDPP
                     PVLFVRGKLLPQDKQAVAIVGARSQTHYGESLTENLAGALAQNGVTVVSGFARGIDAV
                     AHRAALKSGGRTIAVLGCGLDVLYPPEHRELREAIAAKGAVISEFPFGTPPDPFNFPR
                     RNRIISGLSLGVVVVEAGSKSGALITAEHALEQGRDVFAIPGNVFAKNSQGTNKLIKQ
                     GAALVTSVEDVLSQLQGLARTQKSRSSTAPLLEPLGLSEEEQHLLRLLSEEPVHIDLL
                     TQKAALSVGQVLAALTNLEFKGVVRQLAGKQFVRQMAPELVP"
     gene            3906..4172
                     /locus_tag="AC16GM_06215"
     CDS             3906..4172
                     /locus_tag="AC16GM_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR2923306.1"
                     /translation="MIEKTVMVTNRLGIHARPAAMLVQKASKFKSEIRLVKGALEVNA
                     KSIMGVMMLAAECGSKLFLRAWGPDAEQAVAELTDIFESKFNEE"
     gene            4181..5923
                     /gene="ptsP"
                     /locus_tag="AC16GM_06220"
     CDS             4181..5923
                     /gene="ptsP"
                     /locus_tag="AC16GM_06220"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR2923307.1"
                     /translation="MKVYRGIPAAAGMAIGRATVWGTPEPSAVSRTIRSADVAGEKAR
                     LARAVERAQAELRRMAQKLPKTDPGVKLLLAQQMMVTDPEILKETEAVISSHFVDAAT
                     AYRQAVERVRSSIAATANTYLRDRANDLAGASSLVLKYLSGGERLPRRVKPGQIVLAC
                     NLSPGDVLTLCRQQVRGLVSETGGATSHAAILAKAWGIPAVMGLVNVTKKLGGKGSPV
                     IVDGGPGKVIINPDSKTREEFERRSKREKREQRALLALRRLPAVTRDGHRVRLSANLD
                     FPEETEAARRNGAEGVGLFRTEYLLTNQGIPTEEEQSAVYRKIVRTFAPHPVTLRTFD
                     VGGDKFRRGALPEANPFLGWRAIRIGLDEAAFLATQLRAMLRASTYGRVRILLPMVAT
                     VAEVTAAQKIFAQAKQALQHRNVRFDPETLLGIMIEIPAAALVAEHLAAQVDFFSIGT
                     NDLAQYTLAVDRGNPKVAHLFDPFDPSVLRLLAMSLAATKKQGIEVSVCGEMAADPLA
                     VPLLIGLGVQELSVVPALLPGVKRLIRELRFADLGALASEVLEQASPGAVKAILRRFL
                     EQIEKQGRRRGRLG"
     gene            5907..6962
                     /locus_tag="AC16GM_06225"
     CDS             5907..6962
                     /locus_tag="AC16GM_06225"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HIR2923308.1"
                     /translation="MAGLDDPQVYRTLDPENYYRLIYDFPLQLKEALALSFSAAPEQP
                     FDSIVLCGMGGSAIAGDLVLSLLRYELSLPFVVVRQYHLPKSVTALALVIASSYSGNT
                     EETLAAYDQARARGCPIVALTTGGELLLRAERDGCGYCVLPPGYPPRAALAFSFVPLL
                     GLLTQLRLTHDWREEVQKASEFLRHVREEWNQKSPVAKNLAKQIAEKLYRRIPIIYAS
                     GDRLDAVATRWKGQICENAKTLAFANFFPEFNHNELVGWQVLEGVREKLIALFLEDAG
                     DHPRIRRRSEIVQRVIEGKNVAALTIPSQGTSFLSRLLYLVYLGDLASYYLALLSGVN
                     PKPVAVIDFLKAELVRS"
     gene            7102..8076
                     /locus_tag="AC16GM_06230"
     CDS             7102..8076
                     /locus_tag="AC16GM_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2923309.1"
                     /translation="MKVIIPVAGGGTRLWPLTQRTPKVLLQVADRPMLGHILKQVEKV
                     EPEEVIFIIGAGGDAIVNYVVRTFDFPVRFVEQTDLIGLGYAVALASPFFGRGPLLVI
                     LGDTLIGADLKKIMNIGHDALGVKEVDDPERFGIAEVTRGVITRLVEKPQEPQSNLAL
                     VGLYFIRSVELFSNCLAELLAGGVRTGGEYQLTDALQKMIERGCQFRSFPIHEWLDCG
                     KPETLLATNRHLLRNGSDNAGPKTKGIIPPVFVAPDAVVTGSRLGPFVSIGSRARIKD
                     AVLADVICGSEASVSGVTLEHAIVGNGATVHGQIPVRKTKDEILLIQN"
     gene            8092..9255
                     /gene="metK"
                     /locus_tag="AC16GM_06235"
     CDS             8092..9255
                     /gene="metK"
                     /locus_tag="AC16GM_06235"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR2923310.1"
                     /translation="MPRKNFLFTSESVTEGHPDKVCDQISDAVLDEVIRQDRTGRVAC
                     ETFITIGLVIVGGEITTKGYVDVPNLVRNVIRDIGYTDPKYGFNCDTCAVLNAIGAQS
                     PDIAQGVDTGGAGDQGQMVGYACRETPELMPLPIALAHRLTRRLAEVRKKKILPYLGP
                     DGKAQVTVQYDNGTPAKVTSVVLAAQHTEEILDATGKKITEEARQEIIEAVVKPVIQP
                     ELMDKQAKLYVNATGKFVIGGPQCDTGMTGRKIIVDTYGGMAAHGGGAFSGKDPSKVD
                     RSASYMARYIAKNIVAGGLAERCTVQLAYAIGVAEPVSVMVDTEGTGKLDDLRLTEMV
                     KMHFALTPRGIIEALNLLRPIYQKTASYGHFGRDDADFTWEKTDKIKDLQKEA"
     gene            9304..10557
                     /locus_tag="AC16GM_06240"
     CDS             9304..10557
                     /locus_tag="AC16GM_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR2923311.1"
                     /translation="MRYDVKDKKLARTGKLRVEWAERNMPVLRLIRKRFAREKPLKGL
                     RIAACLHVTTETATLMETLVAGGAQVALCASNPLSTQDDVAAYLVTRRIPVFAVKGEN
                     NKTYYRHIQDTLNIKPQITMDDGADLVSTLHTERPEEAEKILGGTEETMTGVTRLRSM
                     ERSGVLRYPIVAVNDSKTKYLFDNRYGTGQSTIDGILRATNMLLAGARVVILGYGWCG
                     RGVAMRAKGMGADVIICEVNPHRALEAVMDGYRVMSSGEAAREGNLFVTVTGDINVLR
                     SEHFRAMNDGAVVANAGHFNVEIDLASLVKLARRRRAVRQYIEEFTLNGGKKVNVLAD
                     GRLINLSAAEGHPAMVMDMSFANQALSVEFIAKKGGELERKVYVVPEEIDRQVAELKL
                     KSMGVRIDRLTEEQKNYLSSWEMGT"
     gene            10605..11201
                     /locus_tag="AC16GM_06245"
     CDS             10605..11201
                     /locus_tag="AC16GM_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR2923312.1"
                     /translation="MLPPAETYALLRHLTTPLVAVTTRWRERRNGLVATSAQRASLSL
                     QMPRLSVSIHKWTYTYELLWKSGRFAVHLLHPGQLELVHLLGFTSGREVDKLAGVACH
                     TGFSGSPILNDCFAWFDCRVVNVMDAGAYTCFLGDVVAAGRGPGAEVLTSEKFRSVMP
                     ESWQREYALRLARAQEWATAHSQIEQTNWPGPEDEDQG"
     gene            complement(11210..>11512)
                     /locus_tag="AC16GM_06250"
     CDS             complement(11210..>11512)
                     /locus_tag="AC16GM_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR2923313.1"
                     /translation="KERSGELATREKVDLHLKLAFPLINFISVLFGAPLVTNPRKSGL
                     ALSFGITLTIAFLYYIASKFVQVAGYNQKLPPFVAAWLTNAVFGISGFILLWRARK"
BASE COUNT         2360 a         3281 c         3615 g         2256 t
ORIGIN      
        1 aattaaagac cacttcacac caattgatta ctcagtacga atgaaagttt ccccagagaa
       61 tgcagctcca aaaaagaaat cgaagcgcgg tgtcatccgc tgcggcgagc aagagattaa
      121 tctcttctcc cgcacgcaca ttatgggtgt gctcaacgtc acgcccgact ccttctacga
      181 cggcggccgg tactacgacc taaggcgggc agtcaaccgc gcgcgcgaaa tgaccgacga
      241 aggggccgat ttcatcgaca tcggcgggct ttcgacccgg cccggctccg agccggtttc
      301 cgaagaagaa gaaatcaagc gggtgattcc cgtcgtaacc gaggtcgcga cgcaggtgtc
      361 aatccccctt tcgattgata cttaccgcgc cagcgtggcc cgggccgctc tggatgctgg
      421 cgcctctctg gtgaacgaca tcagcgggct ggcttttgat ccggcgatgc cgaaactcgt
      481 agcggaaagc ggctgtccgg tcgtcatcat gcacatcaag ggcacgccgc gcgacatgca
      541 ggtcgatccg cactacgacg acgtcattgc cgaaatcagc cggcatttcc gggaccgcat
      601 cgcctccgca aagtcagccg gcattgctga cgaacagatt ctcttagacc ccggcatcgg
      661 cttcggcaaa agaatggaag ataacctggt tattctccaa cgactgcgcg acttcgcgga
      721 cttgggctac ccgcttttgg tgggcgcgtc gcgcaagagt ttcatcggca aaattttaga
      781 cctcccctct gcagaacgtc tggaagggag cttggccgcc gcggcggtgg ccatccagaa
      841 cggcgccgcc atcatccgca cccacgatgt taaagccacc gtgcgggtgg cacggatcgt
      901 cgatgcaatc cgccggtcag gttgagttgc atctctccgt aaatctccct atactttttg
      961 cgtgaacttt ttcacgttcg gctttttgaa attcggcctg gtcgatgccg tggacatagg
     1021 cctcgtcagt tttgtgacct ataaactcct ctcgctcatg aaggggacgc gctcagccca
     1081 gattttgacc ggcctatttc tcgtgatcgc cgtgtcgttt ctggcgttct ggttccagtt
     1141 ggaagcggtg aagtggctca tcacgaacat cgccaccgta gggcttctgg tattggtcgt
     1201 cctcttccaa ccggaaatcc gcggggcgtt ggcgcagatc ggccacaacc gggtggtacg
     1261 gctcttctac aagtatgagg agcagaaatc gttggatgaa atcgtgcggg ccacgcagaa
     1321 gctggccgag tccaagaccg gcagcttgat cgttctggag cggcaggtag ggttgaaatc
     1381 gtacatcgaa tcggggcgcc tgatgcacgc gcaggtgtcc gaagaacttt tgataaccat
     1441 cttctcgccg cgggcgccgt tgcacgacgg cgcggtcatc atccggggcg acttagtggt
     1501 ggcggccggc tgcaccctgc ccctttcgcg gacgctggag ttcaccgccc cgcacgggat
     1561 gcggcacaaa gctgccaccg gcatcaccga agaatcggat gccattgccg tcgccgtatc
     1621 cgaggaaacc ggcgaaattt cggtggccgg ctgggggcgg ctggagcggg gaatcgatcc
     1681 ccatgcgctc cgggaccacc tggtgcgcct cctgcggagc cagtaaggtg ctcgcgccga
     1741 cgggtgccga gagcgattca ttgcggccat atggtttgcg cataacagag ttatgttcgt
     1801 tgactacgtt gaaatcacag tcaaggcggg tgacggcgga cacgggtgtc tctcgttccg
     1861 gcgcgagaag tatgtgccca aaggcggccc ggacggaggg gacggcgggc ggggcgggca
     1921 cgtgatcgcc cgggttgatc cacacctggc cacactttta gatttgcgct accagagact
     1981 ctaccgggcg gccaaggggg gcgacggtca ggggaaagat ttgttcggca gggaagggga
     2041 agaccgggtc attccggtgc cgccggggac gaccatcaaa gacctcaaca ccggaaaagt
     2101 cattgccgat ttgacccttc cggatcagga agtgcttttg gccagaggcg gccggggcgg
     2161 gcacgggaac gcctttttca aatcctccac caaccgggcg ccgcgccgct ttgaggcggg
     2221 taaaaaagga gaagagaagc gcctgacttt tgaattgaaa ctgatcgccg atgtcggctt
     2281 ggtcggtttc ccgaatgtgg gcaaatcgac cctcttagcg aaagtctcca gagcccggcc
     2341 gaaaatcgcg ccctatcctt tcaccacgct tacgccgcac ttaggcatcg tgcgtgttgg
     2401 cgattggcgc tcattcgttt tggccgacat tcccgggatc atcgccgggg cacatcaggg
     2461 caaagggctg gggctggaat ttttgcgcca cattcagcgg acgcgcgttt tagtttacct
     2521 tctggatgcc accagttcca cgctccgccg cgattaccaa atcttaaagg cggaagtgaa
     2581 ggcctacgat ccggaaatca cccggaagcc gtcgctcgtg gtgctaaaca agatcgatct
     2641 cctcaacggc cagcccaaga agacgttgcg ggtgtcgatg ccggtttttt acatctcggc
     2701 tttgaccggc caaggaatca aggagttgct tttcggcctg gaggaggtcc tgggttcagt
     2761 ttcccgggcg gcctgagtat ggcgctgacg gacgcggaaa aagaaaactg gctggcgctt
     2821 tcgctcgtgc cgaatgtcgg gcctttgcat ttcaccgcgc tggtgaaacg ttttggctct
     2881 gccggtgccg ttctggaggc cggcgagaag tcactcctcg aagttccggg cgtcgggtta
     2941 aagaccgccg cagcgatacg gcgcacgccg gtgcgggagg aagcccggaa gcaactggct
     3001 cttttgcaga aagaaaagtg tgacctcatc acgttagccg atgagagcta cccgttcaat
     3061 ctcaaaaata tctccgatcc gccgccggtg ctcttcgtcc ggggaaagct tttgccgcag
     3121 gataagcagg ccgtggccat cgtcggcgcc cgctcccaga cccactacgg ggagtccttg
     3181 accgaaaatc tggccggggc gctggcccag aatggcgtca cggtggtgag tggttttgcg
     3241 cgggggattg atgccgtggc gcaccgggcg gccttgaaat cgggcgggcg aacgatcgcc
     3301 gtcctgggct gcgggttgga tgtgctctac ccgcccgagc acagagaatt gcgcgaagca
     3361 atcgccgcta aaggcgcggt catttctgag tttccctttg gcacaccgcc tgatcctttc
     3421 aattttcccc ggcgcaaccg gatcatcagc gggctgtcct tgggcgtcgt cgtcgtggag
     3481 gccggatcga aatcgggcgc gctcattacc gccgaacacg ccttagagca gggacgcgac
     3541 gtcttcgcca tccccgggaa tgtcttcgcc aaaaacagtc aggggacgaa caaattgatc
     3601 aagcaaggag ccgcgctggt cacgtcggta gaggacgtgc tgtcgcagct gcaggggctg
     3661 gcccggacgc agaaatcccg gagcagcacc gcgccgctac tcgagccgct tggtctttcg
     3721 gaagaggaac agcacttgct gcggctcctt tcggaggaac cggtgcatat cgacctttta
     3781 acacagaagg cggcgctctc ggtggggcag gtcctggcgg ccctgacgaa tctggagttc
     3841 aagggggtgg tgcggcagct ggcggggaag cagttcgtgc ggcagatggc accggaactg
     3901 gtcccgtgat cgaaaaaacg gtgatggtga ccaaccgtct gggcattcac gcccgcccgg
     3961 cggcgatgct ggtgcagaag gcgagcaaat tcaagagtga aatccggctg gtaaagggag
     4021 cgctcgaagt aaacgccaaa agcatcatgg gggtgatgat gctcgcagcc gagtgcggca
     4081 gcaaactttt cctccgcgcc tggggtcccg atgccgaaca ggcggtggct gaactgacag
     4141 atatttttga aagtaagttc aacgaagaat gagcggaaaa atgaaggtat accgggggat
     4201 tccggcggcg gcggggatgg ccatcggtcg ggcaaccgtt tggggcacac cggagccctc
     4261 ggcggtctcc cgcacgatca ggtccgctga cgtcgccggg gaaaaggcac ggctcgccag
     4321 agcggtagag cgggcccaag ccgaactgcg ccggatggcc cagaaactcc cgaagaccga
     4381 tcccggggta aaacttctcc tggcgcagca aatgatggtg actgatccgg aaattctcaa
     4441 agagaccgag gcagtcatca gctcacattt cgtcgatgcc gccaccgcct accgccaagc
     4501 tgttgaacgg gtgcgcagtt caatcgccgc aaccgcgaat acctacttgc gcgaccgcgc
     4561 caatgacttg gccggggctt cgtctttggt gctcaagtac ctttcgggtg gggagcgtct
     4621 tccccggcgg gtaaaaccgg ggcagatcgt cttggcctgc aatctttcgc ccggggatgt
     4681 cctgacgctc tgtcgtcagc aggtgcgcgg gttggtttcc gaaaccggcg gggccacctc
     4741 ccacgcggcg attctggcca aagcctgggg cattccggcg gtgatgggcc tcgtcaacgt
     4801 tactaagaaa cttgggggga agggttcccc cgtcattgtg gatggaggac ccggtaaggt
     4861 cattatcaat cccgattcaa aaacgcgcga agagttcgag cggcgcagta aacgggagaa
     4921 acgagaacag cgcgcgcttt tggcgcttcg ccgactcccc gcagtgaccc gcgatggtca
     4981 ccgggtgcgg ctatcggcca acctcgattt ccctgaagag accgaggcgg cccgccggaa
     5041 cggagccgag ggggtgggcc tgttccgtac cgagtacctg ttgaccaatc aggggattcc
     5101 gaccgaagag gagcagtcgg ccgtttaccg aaaaatagtc cgcacctttg ctccgcatcc
     5161 ggtgacgctg cggaccttcg acgtgggcgg tgataagttt cgcagaggag cattgccgga
     5221 agccaatccc tttctgggct ggcgggcgat ccggatcggg ctggacgaag cggctttcct
     5281 ggccacgcaa ctgcgggcca tgctccgcgc ttcgacctac ggtcgggtgc ggattctgct
     5341 cccgatggta gccacggttg ctgaagttac ggctgcccaa aaaatttttg cacaagcgaa
     5401 acaagcactc cagcaccgga acgtgcgttt cgatccagag acgcttctgg gaatcatgat
     5461 cgaaattccg gcggcggcac tggtggccga gcacctcgcg gcgcaggttg atttcttcag
     5521 catcgggacc aacgacctgg cgcagtatac cttagcggta gaccggggaa atcccaaagt
     5581 ggcacacctg ttcgatccgt tcgatccgtc agtgctgcgg cttttggcca tgtccctcgc
     5641 cgcaacaaaa aagcagggta tcgaggtgtc ggtgtgcggt gagatggcgg ctgacccttt
     5701 ggcagtgccc ttattgatcg gtctgggggt gcaggaactc tcagtggtcc cggcgctctt
     5761 gcccggggtc aagcggttga ttcgggaact ccggtttgcg gatttaggcg ctttggcatc
     5821 cgaggttctg gaacaggcga gtccgggggc ggttaaagca attctgcggc gctttcttga
     5881 gcaaatcgaa aaacagggga gacgacgtgg ccggcttgga tgacccccaa gtttaccgga
     5941 cgcttgaccc ggaaaattac taccggctga tttatgattt cccgctgcag ttgaaagaag
     6001 ccctggcgct ttctttttcg gcggctcccg agcagccatt cgactccatc gtgctctgcg
     6061 ggatgggagg gtcggccata gccggcgatc tggtcctctc gctcctgcgc tacgaacttt
     6121 cgcttccttt tgtcgttgta cgtcaatatc acctcccgaa aagtgttact gcccttgcgc
     6181 tggtgattgc gtcgagctat tccggcaaca cggaggagac ccttgcggcg tacgatcaag
     6241 cccgggcgcg cggctgtccg atcgtcgcgc ttaccaccgg cggtgaactg ctcttgcggg
     6301 ccgagcgcga cggttgcggc tattgcgtgc tgccgccggg ctacccgccc cgggcggcac
     6361 tggcgttttc gttcgttccg cttctgggac ttctgaccca actcaggtta acccacgact
     6421 ggcgcgagga ggtgcaaaaa gcttctgaat tcctcaggca cgtccgtgaa gagtggaatc
     6481 aaaagtcacc ggttgccaag aatcttgcga agcagattgc cgaaaaactc tatcgaagaa
     6541 ttccaatcat ctacgcgagc ggcgaccgcc tggacgccgt ggcgacccgc tggaaagggc
     6601 agatttgtga aaatgccaag acactggcct ttgccaattt cttcccggag ttcaaccaca
     6661 atgaactggt cggctggcag gtgctggagg gagtgcggga gaagctcatc gctctgtttc
     6721 tcgaagatgc aggcgaccat ccccgcatcc gccggcgcag cgagattgtc cagcgggtga
     6781 tcgaagggaa aaacgttgca gccctcacca ttcccagcca gggcacgagc tttctctccc
     6841 gcctgctgta cctcgtttac ctgggcgatt tggccagtta ctatctggcc ctgctcagcg
     6901 gggtcaatcc caaaccggtc gcggtaattg atttcctcaa agctgaattg gtccggtctt
     6961 gagcgacggt aaaagcagcg ggaatgtgct tgagcacgtc gtctctgtag ctcggcttgc
     7021 gtaattctct tacaaagagt aaattgtccg cgcttcccga acagttatcc aactggaagg
     7081 cgaagaggca gttgatctat tatgaaggtg attattccgg tggccggagg cggaacgcgg
     7141 ctctggccgt taacgcagag aacgcccaag gtgctcctgc aggtagccga ccggccgatg
     7201 ctgggacata tcttgaagca ggttgaaaag gtggaacccg aagaagtgat cttcattatc
     7261 ggcgccggcg gcgacgccat cgtcaactac gtggtgcgca ccttcgattt tccggtgcgc
     7321 ttcgtggagc agaccgattt gatcggcctg gggtacgcgg tggctctggc gagtcctttt
     7381 ttcggccgcg ggccgcttct cgtcattctc ggcgataccc tcatcggcgc ggatttgaaa
     7441 aaaatcatga acatcggcca cgatgctctg ggcgtcaaag aggtagacga tccggagcgc
     7501 ttcggcattg cggaagtgac gcgcggcgtg attacgcggt tggtggaaaa gccgcaggag
     7561 ccgcagtcca acctggcttt ggtcggtctg tacttcattc ggtcggtcga actcttctcg
     7621 aactgcttag cggagctatt ggccgggggc gtccgcaccg ggggggagta ccagctgacc
     7681 gatgcgctgc agaagatgat cgagcgcggc tgtcagttca gatcgtttcc aattcacgag
     7741 tggctcgatt gcggcaagcc agaaacgctt ttggccacca accgccatct gctgcggaac
     7801 ggttcggata acgcgggacc gaagacgaaa ggtatcatac cgcccgtttt tgttgcaccc
     7861 gatgcggtgg tgacgggctc ccgcctgggg ccgttcgtct cgatcggcag ccgcgcccgg
     7921 atcaaagacg cggtgctcgc ggacgtcatc tgcggcagtg aggcttccgt cagcggcgtc
     7981 actctggaac atgccatcgt tggcaacgga gcgaccgttc acggccagat ccccgtccgc
     8041 aaaacgaaag atgaaatcct gctcatccag aactaacctt caggagaggg aatgcccagg
     8101 aaaaatttct tattcacttc agaatcggta accgaaggcc atcccgacaa agtctgcgac
     8161 cagatttcgg acgcggtctt ggacgaagtg attcgccagg accggacggg ccgggtggcc
     8221 tgtgaaacct tcatcaccat cgggctcgtg atcgtcgggg gtgagatcac caccaaaggg
     8281 tacgtggacg tgccgaattt agtccgcaac gtgatccgtg acatcggcta caccgacccg
     8341 aagtacggct tcaactgcga cacctgcgcg gtgttgaacg ccatcggggc gcagtcgccg
     8401 gacatcgccc agggtgtcga taccggcggg gcgggagatc aggggcagat ggtgggctac
     8461 gcctgccggg aaaccccgga gctgatgcct ttacccattg ctttagccca ccggctgacc
     8521 cggcgtctgg cggaagtgcg caagaaaaaa attctcccgt acctgggccc ggacggcaag
     8581 gcgcaagtaa cggtacaata cgacaacggc acgcccgcca aggtgacctc cgttgtcctg
     8641 gccgcgcagc ataccgaaga aattctggac gcgaccggca agaagatcac cgaagaagcg
     8701 cggcaggaaa tcattgaggc cgtggtcaag ccggtcatcc aaccggagct gatggataag
     8761 caggcgaaac tctacgtgaa tgccaccggt aagttcgtca tcggcggccc gcagtgcgac
     8821 accggcatga cggggcgcaa gatcatcgtc gatacctacg gcgggatggc ggcccatggc
     8881 gggggcgctt tctccggcaa agatccctcg aaggtcgacc gttccgcatc ctacatggcc
     8941 cgctacatcg ccaaaaacat cgtggctggg gggttggccg agcgctgcac cgtgcagtta
     9001 gcctacgcca tcggtgtggc cgagccggtc tctgtcatgg ttgataccga agggaccggc
     9061 aagttggacg acctgcgcct gaccgaaatg gtcaaaatgc attttgccct caccccgcga
     9121 ggaatcattg aggcgctcaa cctcctgcgg ccgatctatc agaaaaccgc gagctatggt
     9181 cacttcgggc gggacgatgc cgatttcacc tgggaaaaga cggataagat caaggattta
     9241 cagaaagagg cttgagagcg cgagcgtgaa gtcggagtgc cgcgaaaacg aaagcgagga
     9301 gtaatgcgtt acgatgtgaa ggataaaaaa ctggcccgta cggggaaatt gcgggtggag
     9361 tgggcggaac ggaacatgcc ggtcctccgc ctcatccgca agcgttttgc gcgggagaaa
     9421 ccgctcaaag ggctgcgcat cgccgcctgc ctgcacgtca ccaccgaaac ggccacgtta
     9481 atggaaacgc tcgtggccgg cggagcgcaa gtggcgctct gcgcttccaa ccccctctcg
     9541 acgcaggacg acgtggcggc ctacctggtg acccggcgga tcccggtctt tgccgttaag
     9601 ggggaaaaca acaagaccta ctaccggcac attcaagaca cgctcaatat taaaccgcag
     9661 attaccatgg atgacggcgc cgatctggtc tcgaccctgc acacggagcg acccgaagaa
     9721 gccgaaaaga tcctcggcgg caccgaggag acgatgacgg gcgtcacccg cctgcggagc
     9781 atggaaaggt cgggggttct gcgctacccg atcgtcgcgg tcaacgactc gaagacgaag
     9841 tacctgttcg acaaccgcta cggcacgggt caatcaacca tcgacggcat tctgcgggca
     9901 actaatatgc ttttggcggg tgccagggtg gtcatcctgg gctacggctg gtgcggccgg
     9961 ggtgtggcga tgcgggcgaa aggaatgggg gccgacgtca tcatctgtga ggtcaatccc
    10021 caccgggcgc tggaagcggt gatggacggg taccgggtca tgtcttcggg cgaggccgcc
    10081 cgcgaaggca atctatttgt caccgtcacc ggcgacatca atgtgctgcg ttcggagcat
    10141 ttccgcgcga tgaatgacgg agcggtggtg gccaacgccg ggcacttcaa tgtcgaaatc
    10201 gatttggcgt cactggtgaa attggcccgc cggcgccggg ccgtgcgcca gtatatcgaa
    10261 gagttcacgc tcaatggcgg caaaaaggtg aacgttctgg ctgacgggcg gctgatcaat
    10321 ctttcggcgg cggaaggcca cccggcgatg gtgatggata tgagttttgc caaccaggcc
    10381 ttaagcgtgg agttcatcgc taaaaagggc ggcgaactgg agcgcaaggt ctacgtcgtg
    10441 ccggaggaga tcgatcggca ggtggcggag ttgaaactga agtcaatggg cgtcagaatt
    10501 gatcggctca ccgaagaaca gaaaaattac ctctcctcgt gggaaatggg aacgtaggtt
    10561 attcgcaacc ctcacccacc ggaccacagg gttaggccgc cgtcgtgctt cctccggctg
    10621 aaacgtacgc tctgctccgt cacctgacga cgcctttggt ggcggtcacg acccgctggc
    10681 gcgagcggcg caacggactc gtcgccacca gtgcgcaacg ggcttcgctc tccctgcaga
    10741 tgccccgtct ttccgtctct attcacaagt ggacctacac ctacgaactc ctctggaaga
    10801 gcggccggtt tgcggtgcac ctcctccacc cggggcagtt ggaattggtc caccttttgg
    10861 gttttacgag cggcagggaa gtcgacaagc tcgcgggggt cgcttgccac accgggttca
    10921 gcggcagccc cattctcaat gattgttttg cctggttcga ttgccgggtg gtcaatgtga
    10981 tggatgcggg ggcatacacc tgctttctgg gtgacgtcgt agccgccggg cgcggaccgg
    11041 gagcggaagt gctcaccagc gaaaagttcc gcagcgtcat gcccgagagc tggcagcgtg
    11101 aatacgcgct acggttggcc cgcgcgcaag agtgggccac cgcacattcc cagattgagc
    11161 agactaactg gcccggtccg gaagacgagg atcaaggata gcccggattc tacttccggg
    11221 cgcgccagag caaaataaat ccgcttatcc cgaaaacggc attcgtcagc caggcggcga
    11281 cgaagggcgg cagtttctgg ttatacccgg cgacctggac gaacttgctc gcgatgtagt
    11341 agaggaaggc gatggtcagg gttatgccga agctcaaggc caggccgctt ttccgcgggt
    11401 tggtcaccag gggcgcgccg aaaaggacgc tgatgaagtt gatcagggga aaggcgagtt
    11461 tcaggtgcag gtcgactttc tctctggtcg ccagttctcc ggagcgttcc tt
//
LOCUS       DBNGZD010000241         7741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1148972, whole genome shotgun sequence.
ACCESSION   DBNGZD010000241 DBNGZD010000000
VERSION     DBNGZD010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1148972"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..814
                     /locus_tag="AC16GM_06255"
     CDS             119..814
                     /locus_tag="AC16GM_06255"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR2923314.1"
                     /translation="MDESEIDVRRRLEMVETQIAKRGIADPLILAAMRQVPRHFFVPE
                     KFHALAYADEPQPIGYDQTISQPYIVGLMTELLGLKGGERVLEIGTGSGYQAAILAEI
                     AGVVFTMEIVRQLAERARERLRELGYVNIWCKIGDGSQGWPEHAPYDAIILTAAPEEI
                     PRPLLDQLKIGGRMVLPKGTAEQDLYLVEKTKAGLVEKNIIPVRFVPMTGKTDRRFQS
                     DRPSGVDPNSDQR"
     gene            968..1444
                     /locus_tag="AC16GM_06260"
     CDS             968..1444
                     /locus_tag="AC16GM_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR2923315.1"
                     /translation="MAQRLYRSRKETMISGLCGGIAEYFDIDPTWIRLVFVLLIFAGG
                     AGIIAYLIGMIIVPKAPLDAGQPPPVAAGGVGGSTGASAASAPAPAPTGSPPAATSGT
                     PPARAEYLIGGILVLVGLFFLFENLFWFPFHRLWPILLIVIGAILIIKAMDRGDAR"
     gene            1441..2367
                     /locus_tag="AC16GM_06265"
     CDS             1441..2367
                     /locus_tag="AC16GM_06265"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaI-LiaF-like domain-containing protein"
                     /protein_id="HIR2923316.1"
                     /translation="MSPRMLRNGLLLIYIGVIFLLINLDYVNWDFWRPILRLWPLLLI
                     AIGLEKILLAAKLRPVAYLAPVGLMAVLTWVAWVNYPRYARGGEERVARMVVEPTPEY
                     RRVRLNLDVSRPDIVLEAASNHVLNAECDYLFHAPELSTETSDSMLTLTLKEHDRFFA
                     QAGPFFFPRNEWDVALTDALPLELTLTCNHADHEFDFRQFRLTAFTYRGRKGDLKLCV
                     GDRVPQVTITLDAPKVECEIGVPEQAGVRVKCSRLLSDRLLEKNGLQKEGDFYTTTNY
                     AKASPKIEIDLGGGVGDFRLRTGECPFTFRGD"
     gene            2533..3336
                     /locus_tag="AC16GM_06270"
     CDS             2533..3336
                     /locus_tag="AC16GM_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR2923317.1"
                     /translation="MRSVLRILPLLIVAGFVCTCSKRTADTFARIQKTRILRVGNDPT
                     YPPFESKDTHTGKIVGFDIDLIDAICTKLDVRAEYVEAPFDGIIAGLNTDKYDCVISS
                     MSIKPERAQVVDFSLPYYQAGQAIAVRLKDEKIQGVEDLKGKRIGCQLGTTGETFART
                     QIPDGQVISFDAISQAFLDLENGKLDAIINDRPTTERFIALKGTAKIVGPTLTSEDYG
                     IAVRKGDARLLTAINNALNSLRTAGKFDEIKAKWFGAPRAGQPAKTPAS"
     gene            3420..3496
                     /locus_tag="AC16GM_06275"
     tRNA            3420..3496
                     /locus_tag="AC16GM_06275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3454..3456,aa:Arg,seq:acg)
     gene            complement(3702..4559)
                     /locus_tag="AC16GM_06280"
     CDS             complement(3702..4559)
                     /locus_tag="AC16GM_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923318.1"
                     /translation="MKRWGVLAVGLLLLFLVGCFDLKEEVWINADGSAKIMQDIILPE
                     AMTSLGGEQANLAEQLNKQYGEKKSRLEADPRIKSVDTRHFTEGDKHHFVLEVEIKDV
                     TDLPEIWPLAFANKPDAAGKGSDSPLANQENRLSVSKTGDGNIHLTRVLKSAEKTGKK
                     DSTNQMGDAMGQAMAAQLFANNGITFTVHGSKIAQANGTILDDGQTAEWKIPLTEMMG
                     KNFSKEMSAEIGPGGAGLPTWIPIAAGGFLVLLVLLILITRNRKKKSSRFDNVPVIRP
                     STQKPVGVQ"
     gene            4820..5302
                     /gene="tadA"
                     /locus_tag="AC16GM_06285"
     CDS             4820..5302
                     /gene="tadA"
                     /locus_tag="AC16GM_06285"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR2923319.1"
                     /translation="MSHLLSGNNDETWMQAALKEAARAFDKGEVPVGAVAIYKNQIIG
                     RGHNQVETLKDATAHAEILALTAAMQYLDSWRLEEVTLYATLEPCCMCAGAVVLARVP
                     RLVFAAFDPKAGACGSLFNLVADPRLNHRVVVAHSVSAEESQALLQEFFRKLRVEEKS
                     "
     gene            5340..5813
                     /locus_tag="AC16GM_06290"
     CDS             5340..5813
                     /locus_tag="AC16GM_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923320.1"
                     /translation="MNSKPFDSDALHAKLAYLGLAIDVTLPIVLLAVGYAIRKDQPSA
                     LDYPTLRLLWVVLLAIAAGEFAVGLVLRKIWFAPQRFRQAVERAGGRADAVILRYAIA
                     LYAVSAAPAVYGFIYYLLGGTIEQFVGFLAITLLCYQILRPRPGLVDPLLKNSLS"
     gene            5974..6060
                     /locus_tag="AC16GM_06295"
     tRNA            5974..6060
                     /locus_tag="AC16GM_06295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6008..6010,aa:Ser,seq:cga)
     gene            6221..6838
                     /locus_tag="AC16GM_06300"
     CDS             6221..6838
                     /locus_tag="AC16GM_06300"
                     /inference="COORDINATES: protein motif:HMM:NF020496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HIR2923321.1"
                     /translation="MKLAKLRETKSTIRFSAKLFRPKATEKIGSWTLLTLPKNASAKL
                     PSRGMTMVEGTINGFPFRAALEPNGKGSHWLRVNKAMHDAAGADAGDTVTVEITRAGE
                     EPETRVPMDLRKALAVAPPAHASWTDITPMARRNWIFSISSAKQPETRRRRIEKACDM
                     LASGKRRLCCFPGIKWLMKKNAKSCGMWLPLPNSKNRFSPRLPKG"
     gene            6864..7310
                     /locus_tag="AC16GM_06305"
     CDS             6864..7310
                     /locus_tag="AC16GM_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR2923322.1"
                     /translation="MNFMQKMRFPIEIKAPREKVWNTLWEDKTFREWGNIIDEGQYMV
                     GEMKEGNEVQFISPVSGYGVTSLIEKLTPNEFVLFRQIMDTKESGTQEREKEWTGGAE
                     SYSLVEKDGVTTLVVELDVPRGQEETFRVRFPKALERVKILAEKKD"
     gene            complement(7476..>7741)
                     /locus_tag="AC16GM_06310"
     CDS             complement(7476..>7741)
                     /locus_tag="AC16GM_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2923323.1"
                     /translation="TKEAMTALISELDSGHFAISGKGIFKEEFVTCGGVNLKEVDFKT
                     MQSKLCPGLYLAGEILDIDGITGGFNFQSAWTTGWIAGANMAW"
BASE COUNT         1698 a         2143 c         2142 g         1758 t
ORIGIN      
        1 gggaggagaa ggagggatcg ttttgttttc ttgcggtttc ccggcgactc ctcagttgac
       61 gcccgttcgt cattgcccta tcatcactcc gcagcgcctt gcaagcgggg agctccctat
      121 ggacgaatcg gaaatagacg tccgccgccg tctggagatg gtggaaaccc aaatcgccaa
      181 gcgagggatt gccgaccccc taattttagc ggcgatgcgc caggtacccc gccatttctt
      241 cgtcccggaa aaattccatg ccttagcgta tgccgacgaa cctcaaccca tcggctacga
      301 ccagacgatc tcccagccgt acatcgtcgg cttgatgacc gaacttttgg gcttgaaggg
      361 cggcgagcgg gtgctggaaa tcggcaccgg ctccggctac caggcggcga ttttggccga
      421 aattgccggg gtcgttttca ccatggaaat cgtccgccag ctggcggagc gcgcccggga
      481 acgattgcgg gaattgggct acgtcaacat ctggtgcaaa atcggcgacg gctcacaggg
      541 atggcccgag cacgcgccct acgacgccat catcctgacc gccgcgccgg aggagattcc
      601 ccgccccctg ctcgaccagt tgaaaatcgg cggccggatg gtgctcccca agggaacagc
      661 cgagcaggat ttatatttgg tggagaagac caaagcgggg ctcgtcgaaa aaaacatcat
      721 tccggtccgc tttgtgccga tgacgggcaa aaccgaccgc cgctttcagt ctgatcgccc
      781 ctccggcgtt gaccccaaca gcgatcaacg ataacttcct tctcgaatct tatgctggag
      841 agtgaaacac gggacgaacc tgttctccga tgaaacttct tcgcggcggg cgtcgtacag
      901 aagcgtagtt acaaatgtta accgggggaa tatgcgggaa ccgcacgacg aactgaaagg
      961 atagcccatg gcccaacgac tctaccgctc acgcaaagaa acgatgattt ccggcctctg
     1021 cggggggatt gccgagtact tcgacattga cccgacgtgg atccggctgg tctttgtcct
     1081 tttgattttc gccggcgggg cgggcattat cgcctatctg atcgggatga ttatcgtccc
     1141 gaaagcacca ttggatgcag gccagccgcc acccgttgcg gcgggcggtg ttggcggctc
     1201 aactggcgcg agtgcggctt cggccccggc tcctgcaccc accggctccc ctccggcggc
     1261 cacttctggc actcccccgg cccgcgccga gtacttgatc gggggaatct tagtcttggt
     1321 cggtcttttc tttctgtttg aaaatctctt ctggttcccc ttccaccgtc tctggccgat
     1381 actcttgatc gtcatcggtg cgattttgat catcaaggcc atggataggg gagatgcgcg
     1441 atgagcccca ggatgctccg gaacggactt ttgctcatct acatcggggt gatcttcctc
     1501 ttgatcaatc ttgattacgt caactgggac ttctggcggc ccatcctccg cttgtggcca
     1561 ttgcttttaa tcgccattgg tctggaaaag attcttttgg cagccaagct gcggccggtg
     1621 gcttacctgg cgccagtagg gcttatggcc gtattgacct gggtggcctg ggtcaactac
     1681 ccgcgctatg cgagaggggg tgaggaacgg gtggcccgga tggtagtgga accgacacct
     1741 gagtatcgcc gggtgcgcct gaacctggac gtgagccgtc cggacatcgt gctggaagcg
     1801 gcgtccaatc acgtcctcaa cgccgaatgt gactatttgt ttcacgcgcc agaattgtca
     1861 accgaaacct ccgattcgat gctcacgttg accctcaaag agcacgatcg atttttcgcc
     1921 caggcggggc cattcttttt tccccgtaat gaatgggatg tggctcttac cgacgcgctc
     1981 cccttggagc ttaccctcac ctgtaatcac gcggaccacg aatttgactt ccggcaattt
     2041 cgcctgaccg ccttcaccta ccggggccgg aagggcgatc tcaaattgtg cgtcggcgac
     2101 cgcgtccctc aggtcactat tactctggat gcgccgaaag ttgaatgtga aatcggcgtg
     2161 ccggagcaag ccggggtgcg ggtgaaatgc agccgtcttc tctccgaccg cctgctggag
     2221 aaaaacggtc tgcaaaagga aggagatttc tatacgacca cgaactatgc caaggcctca
     2281 cccaaaatcg agattgacct tggaggcggc gttggagatt ttcgactccg aactggcgaa
     2341 tgccctttta cttttcgcgg tgattgattt ttttgcagat cgggaggaac agttgggaac
     2401 tcgcgtcctg acccctccct caccctcccc tcaaggggag ggaaggtcat taccacttcc
     2461 tcccgagagg gaaagtttcg ctaccgaatt ctttcactgc cgcctatctt agagaatatc
     2521 gaagggagga acatgcgttc ggtcttgcgc attttgccgc ttttgatcgt cgctggtttc
     2581 gtctgcacgt gcagtaagag aaccgccgac acttttgccc gtattcagaa gacgagaatc
     2641 ctgcgggtgg ggaacgaccc gacctatccg ccgttcgaat cgaaagacac ccacaccggc
     2701 aaaatcgtgg gcttcgacat cgatctgata gatgccatct gcacgaaatt agacgtgcgg
     2761 gcggagtacg ttgaagcgcc ctttgacggc attatcgccg gattgaacac cgataaatac
     2821 gactgcgtca tctcctcaat gagcatcaag cccgagcggg cgcaggtggt cgatttttcc
     2881 ctgccatact atcaggccgg gcaggcaata gcggttcggc tgaaggacga gaagattcag
     2941 ggggtagagg acctcaaggg caaacggatc ggctgccagt tgggcaccac cggtgagaca
     3001 tttgcccgca cccagattcc tgacggccag gtcatctcct ttgacgccat cagccaggcg
     3061 ttcctcgacc ttgagaatgg aaaactcgac gccatcatca acgaccggcc cacgacggag
     3121 cggttcatcg cgctcaaagg cacggccaaa atcgtgggcc cgactctgac cagcgaagat
     3181 tacggcatcg ccgtccgcaa gggagatgcg cggcttttga cggccatcaa caatgctttg
     3241 aactcgctcc gcactgcggg gaaattcgac gagattaagg ccaagtggtt tggtgcgcca
     3301 agggcggggc aaccggcaaa aacccccgcc tcgtaacctc gcggcggcgc agttcggtcg
     3361 cttggccgga cagatttcgc ttttgattcg tttggccggg atttatattt gccttcgccg
     3421 tccgcgtagc tcaattggat agagcatcag actacggatc tgagggttgg gggttcgact
     3481 ccctccgcgg acgccaattt tatgagaagt atttcccaca ctcaggcctt cgactccgct
     3541 caggctgaag agagcgttgc taccgcctgg tttttgcggg ctaacttcta gtagcgacgg
     3601 tctctgaccg tcggggttac atttttccgg gtcagtcaga ggttagcgca gttcggctgc
     3661 gctcccaagc ccacgtaact tcacgggaga gggaacggca cctactgtac gccgacgggt
     3721 ttttgcgtcg aaggacggat caccgggacg ttgtcaaaac gggacgattt ctttttccgg
     3781 ttccgggtaa taagaatcaa aaggaccaaa agaaccagga agccgccggc agctatcggt
     3841 atccacgtcg ggaggccggc gccccccggc ccgatttcgg cgctcatttc ctttgaaaag
     3901 tttttgccca tcatttcagt caaagggatt ttccactccg ccgtttggcc gtcatccaaa
     3961 atcgttccgt tggcttgggc aatcttcgac ccgtgcaccg tgaaggtgat cccgttattg
     4021 gcaaagagct gtgccgccat ggcctgaccc atggcgtcgc ccatctggtt cgtggaatct
     4081 ttcttacccg ttttttcggc tgacttcagt acccgcgtca ggtgaatgtt gccgtcaccc
     4141 gttttgctga ccgaaagccg gttctcttga ttcgccaatg gcgaatccga tccttttccc
     4201 gccgcatcgg gcttattggc aaacgccagc ggccagatct ccgggaggtc ggtcacatct
     4261 ttgatttcca cttcgaggac gaagtggtgt ttgtcgccct cggtgaaatg ccgggtgtcg
     4321 acggacttaa tgcgcggatc ggcttccaga cggctcttct tttccccgta ctgtttattt
     4381 aattgctccg cgaggttcgc ctgctctccc cccagagagg tcatcgcctc gggcaggatg
     4441 atgtcctgca tgatcttggc cgagccgtcg gcgtttatcc agacttcttc tttcaaatcg
     4501 aagcagccga cgaggaaaag caagagaaga ccaaccgcta aaacacccca ccgtttcata
     4561 acgcctcctt tgtgttaccg atgtttttgg tatcggagta cttacacatt tcttgagagt
     4621 ttacactggc gttaaactac ctttgaagac ctcaccccac cctctcctac aggaaggagt
     4681 ataacttccc tatgaatgaa ggggcaaaat tccctccccg ttaggggaag ggcgagagag
     4741 aggtcggctt ccttcctgta ccaccactct gagccgccga tcttgcagcg gccatttgaa
     4801 gttgtatctt cacggcacga tgagccatct tctctccggc aataacgacg aaacctggat
     4861 gcaggcggcg ctgaaagaag ccgcccgcgc cttcgataag ggagaggtgc cggtaggcgc
     4921 ggtcgccatc tacaagaacc agattatcgg ccgggggcat aatcaagtcg aaaccctcaa
     4981 ggatgcgacg gcccacgccg aaattctggc tttgaccgcc gccatgcagt atctcgattc
     5041 ctggcggctc gaagaggtga cgctctacgc cacgctggag ccctgctgta tgtgcgcggg
     5101 ggcggttgtg ctggcgcggg tgccgcgttt ggtcttcgcc gcttttgacc caaaagccgg
     5161 cgcctgcggg tcgctcttta atctcgtggc cgacccgcgc ctcaaccacc gggtggtggt
     5221 ggctcactcg gtatcagccg aagagtcgca agccctgttg caggagtttt tccgcaaact
     5281 acgcgtggaa gaaaagtctt gactttccct gtgtgccgcg tgctgtttac tgtgtactca
     5341 tgaactccaa accttttgac tccgacgccc tccacgccaa actggcctac cttggcctgg
     5401 ccatcgatgt aactctcccg atagttcttt tagccgtcgg gtacgccatc cgaaaagacc
     5461 agccgtcggc tttggactac ccgacactcc gacttttgtg ggtcgttctc ttggcaatag
     5521 ctgcgggtga attcgctgtc ggcctggtgc tccgcaagat ctggtttgcg ccccagcgct
     5581 tccggcaggc ggtggaacgg gccggcggcc gagccgatgc cgtgattctt cggtacgcca
     5641 tagcactcta cgctgtttct gccgccccgg cggtttacgg ttttatctac tatctgctcg
     5701 gcggcaccat tgagcaattt gtcggctttc tggcgattac cctcctctgc taccaaatcc
     5761 tgcgtccgcg gccgggactg gtcgatccgc tcttaaaaaa ttctctctcg tagtggttca
     5821 cattctttcc gaggcgccag aggattaacc cctcccttgc cctcccccgc cagaggcggg
     5881 gtcttcgacc ctcgaagggg agggaatcag aattggatgg gacagcaagg caacaacctt
     5941 ttccggctgt cttgggcttg ctatactgtg ggtggagagg tgcctgagcg gccgaaaggg
     6001 gcggtctcga aaaccgttgt cccctcacgg ggaccgtggg ttcgaatccc accctctccg
     6061 ttgaaaacac tgagcgaagg cgggaatcgg agcggaaagg caaactgcca aagaacctgt
     6121 caaaaatgtc ggctcaaact ttatgaaggc cagaagcggg cttttcttga ataaaacaga
     6181 aaattagata tgatgatcat acagagtagt ataacaatat atgaaactgg ctaagctgcg
     6241 agaaaccaag tcaacgattc gcttcagtgc caagttgttt cggcctaagg caaccgaaaa
     6301 gattggatcg tggacccttc taactctgcc caagaacgcg agtgcgaagc ttccatcacg
     6361 aggtatgaca atggttgagg gaactatcaa tggcttccct tttcgagccg ctctcgaacc
     6421 aaacggcaaa ggaagtcact ggctcagggt taacaaagcc atgcacgatg ctgcaggcgc
     6481 agacgctgga gacacggtaa cggtggagat tacgcgggcc ggagaagaac cggagaccag
     6541 agtgccaatg gatctacgca aagcccttgc agttgctcca ccggcgcacg catcgtggac
     6601 ggatattaca ccgatggcac gccggaactg gattttctcg ataagctcag ccaagcaacc
     6661 ggaaacgcgc aggcgccgga tcgagaaagc ttgcgatatg cttgcatctg gaaagcgacg
     6721 attatgttgt tttcccggta taaagtggct tatgaaaaaa aacgcaaaat catgcggaat
     6781 gtggcttcca ttgccgaact caaaaaatcg tttttcgcca agattgccaa agggttaggc
     6841 gtttcaatga aagatttgat aaaatgaact ttatgcaaaa aatgcgattt ccaatcgaaa
     6901 taaaagctcc cagagaaaaa gtttggaaca cactttggga ggacaaaaca tttcgtgaat
     6961 ggggaaatat tattgacgag ggacagtaca tggtcggaga aatgaaagaa ggaaacgaag
     7021 tgcagtttat ttcccccgta tccggttacg gcgtgacgag tttgatcgag aagctcactc
     7081 ctaacgagtt tgtattgttt aggcagataa tggatacaaa ggagagtgga acgcaggaac
     7141 gggaaaagga atggactggc ggagcggaga gttattcact tgtggagaaa gacggcgtta
     7201 caactttggt tgttgaactc gacgttccgc gcgggcagga agaaacattt agggtcagat
     7261 ttcccaaagc tttggagcgg gtgaaaattt tagcagagaa aaaggattaa agaatttgcc
     7321 gccaaagaaa aacttgaaat cgtcctttcc gctccggttc aaggcgaggc gggcggaagg
     7381 ggggtgtggg gggaattccg cccgccgagc ccagccagtt cggttcggat attttcaaac
     7441 agacaccgct tgagggcgta aaacgcccgg tccacctacc aggccatatt cgctccggct
     7501 atccagccgg tggtccaggc gctctggaaa ttaaatcccc ccgtaattcc gtcaatatcc
     7561 agaatctccc cggctaaata cagtccggga caaagcttgc tctgcatagt tttgaaatcc
     7621 acttctttga ggttgactcc gccgcaggtt acaaattctt ctttgaaaat cccttttccg
     7681 ctgattgcaa aatgtccact gtctagctcg gatataagtg ctgtcatggc ttctttggta
     7741 a
//
LOCUS       DBNGZD010000242         2181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_593929, whole genome shotgun sequence.
ACCESSION   DBNGZD010000242 DBNGZD010000000
VERSION     DBNGZD010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_593929"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /gene="priA"
                     /locus_tag="AC16GM_06315"
     CDS             <1..904
                     /gene="priA"
                     /locus_tag="AC16GM_06315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR2923324.1"
                     /translation="CGDCGFSFHCPNCSTSLTYHRINQTLRCHFCDRCEKPPDTCPCC
                     RSKKFLYLAHGTQRVEAELKNLFPGTRFERMDTDTTRGKLASFHLLDRFARREFDLLL
                     GTQMIAKGLDFPDVGLVGVVSADLSLHLPDFRAKEKTFQLLTQVAGRTGRGDRPGQVL
                     IQTYAPDEPVIQQAARHDYRSFYQKEIGERLALSYPPFSRLIALIFRSKQEKSAEQVA
                     GLFALKIKREVTRQKMAQIQILGPAPAPFYKLRKEYRWRVTLKAKRVVDILPSIAKIL
                     ESEPAFRPRPLLRITVDVDPQDLL"
     gene            1093..1752
                     /locus_tag="AC16GM_06320"
     CDS             1093..1752
                     /locus_tag="AC16GM_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR2923325.1"
                     /translation="MLTVDDVKKNPTVRTLVEKADKVLETMGYTEHGLRHCSLAANIA
                     HNIMARLKKSEAEAQKAAIAAYLHDIGNLINRDYHAQTGAVMAFHVLSEMGMPLEDRL
                     DITSAIGNHDEKDGYPVSDIGAAVILADKSDVHRSRVRNQDMIKHDIHDRVNYAATSS
                     FLRIDEEKRTASLEITIDTSISQVMEYFEIFLSRMVVCRRAAEFLKLRFELEINGNRL
                     L"
     gene            1908..>2181
                     /locus_tag="AC16GM_06325"
     CDS             1908..>2181
                     /locus_tag="AC16GM_06325"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738351.1"
                     /note="catalyzes the formation of
                     1-(5-phosphoribosyl)-5-aminoimidazole from
                     2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in
                     purine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR2923326.1"
                     /translation="MPSKLTYREAGVDIDAASASVEKVKQLARATFNENVLSEIGSFG
                     GMFAFPGGYKNPVLVSSADGVGTKLKIAFAANRHDTVGIDLVNHCTN"
BASE COUNT          494 a          696 c          542 g          449 t
ORIGIN      
        1 gtgcggcgac tgcggatttt cctttcactg ccccaactgc tcaacttctc tcacctatca
       61 ccggatcaat cagactttgc gctgccactt ctgcgaccgc tgcgaaaagc cgcccgatac
      121 ctgtccctgc tgccggagca aaaaattcct ttatctggcg cacggtacac aaagggttga
      181 agcggaactg aaaaatctct tccccggaac ccggtttgag cggatggata ccgataccac
      241 gcgcggcaag ctcgcttcct tccacctgct cgaccgcttt gcccgccgcg aattcgacct
      301 ccttctgggc acccagatga tcgccaaggg cctcgatttc cctgacgtcg gcctggtggg
      361 cgtcgtctcc gccgacctga gtctccatct ccccgacttc cgggcgaagg agaaaacgtt
      421 tcagttgctg actcaagtgg cgggccgtac cggccggggt gatcgtccgg gtcaggtctt
      481 gatccagacc tacgccccgg atgagccggt catccagcag gcggcgcggc acgattaccg
      541 gtcattttac cagaaggaaa tcggcgaacg actggcgctc tcctacccgc ctttctctcg
      601 tttgattgcc ctgatattcc gctcaaaaca ggagaaatcc gccgagcaag tggcaggcct
      661 atttgcactg aaaatcaaaa gagaagtcac ccgtcaaaaa atggcgcaga ttcagatcct
      721 cggtcccgca cccgctccct tctataagtt gcggaaagaa taccgctggc gggtgaccct
      781 caaggccaaa cgggtggttg atatcctgcc atccatagcg aaaattctgg agagcgaacc
      841 ggccttccgt ccccgccccc tcctgcgcat caccgtggac gttgacccgc aggatttgct
      901 ctgattttct cccggaatcc acgctccaga actcccagaa aatgccgtat taaagatgtg
      961 gagcaatcac ctcccgaaaa gccaggagcg gctatgaatc cgaatgtatc agcgaatact
     1021 ctaactgtcc ctaaccccga gcccaagacc atttccgggc gcaataaaaa ccgcaaccac
     1081 aaaaacggcc cgatgcttac cgtcgatgac gtaaaaaaga accccaccgt ccgcacgctg
     1141 gtggaaaaag ccgacaaagt tctggaaacg atgggctata ccgagcacgg tctcaggcac
     1201 tgttcgctcg ccgccaatat cgcccacaac attatggcgc ggcttaagaa aagtgaagcc
     1261 gaagcccaga aggccgccat cgccgcctac ctgcacgaca tcggaaatct catcaaccgt
     1321 gactaccatg cccagaccgg cgcggtgatg gctttccacg ttctctcaga aatggggatg
     1381 cctctggaag accgtttgga catcacctcc gccatcggca accacgacga gaaggacggc
     1441 tacccggttt cggacatcgg tgccgcggtg attttggccg acaagtcaga cgtccaccgc
     1501 tcgcgggtgc gcaaccagga catgatcaag cacgacatcc acgaccgggt gaactacgcg
     1561 gcgacgagtt ccttcctgcg gattgacgag gagaagcgca cggcatcgtt ggagattacg
     1621 atcgacacct ccatctcgca ggtgatggag tatttcgaga ttttcctctc ccggatggtg
     1681 gtctgccgcc gcgccgccga gtttctgaaa ctccgcttcg aattggaaat caacggcaac
     1741 cgattgctgt aaagccctcc tcctgcaccg gggaggcgcg acttcagtag cgtatcatcc
     1801 ccccgcgtag gcggcactct tagtgccgcc tgtttttgct tttgtagcgc aggttggttt
     1861 acttaacctg cgctataaac cacgtttttg attatattct ttcgcgtatg ccctccaaac
     1921 tcacctaccg cgaggccggt gttgacatcg acgccgcctc cgcctctgtc gaaaaagtta
     1981 aacaattagc ccgcgccacg tttaacgaaa acgtcctctc cgaaatcggc tccttcggcg
     2041 gaatgttcgc cttccctggc ggctacaaaa atcctgtttt agtctccagc gccgacggcg
     2101 tcggcaccaa actcaaaatc gctttcgccg ccaaccgcca cgacacggtg ggcattgact
     2161 tagtcaacca ctgcaccaac g
//
LOCUS       DBNGZD010000243         4045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_594132, whole genome shotgun sequence.
ACCESSION   DBNGZD010000243 DBNGZD010000000
VERSION     DBNGZD010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_594132"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="AC16GM_06330"
     CDS             complement(<1..524)
                     /locus_tag="AC16GM_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstC family ABC transporter permease"
                     /protein_id="HIR2923327.1"
                     /translation="MPATRDGAARNLHIKGGAPPKIDRRKLIERGVELVVYLSSWVSI
                     LGIVFLFIFIGKEAFPLLTDPAMKKEVQFSQLWTSIWQPVSDQPKFGLIPLFLGTLKA
                     SFVALFFATPLAILAAIFTSEFAPPRLGRMIKPAVELLAGIPSVVLGFFALIVLASWI
                     QDAFHLTMRLNATVA"
     gene            complement(528..1385)
                     /locus_tag="AC16GM_06335"
     CDS             complement(528..1385)
                     /locus_tag="AC16GM_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR2923328.1"
                     /translation="MRHRGFLVTAALAFGLGGAGLGLAGKSITVKGSDTMVILGQRWA
                     EIYMQKNPGSVIQVTGGGSGTGIAALINGTTDICEASRPMKDAEKTKLKERYFTLGVE
                     IPVAKDGLAVYLHETNPVEKLSLEQLKAIYTGQITNWNEVGGPDAKIILYGRENNSGT
                     YVFFKDNVLKGADFAPQTATLPGTAAVVNAVAKDKNGIGYGGAAYAKGIKFCKVSEKT
                     DGQAFEPNLATVESGQYPLSRDLYWYLRSRPTGDVKKLVDWVLSPEGQKVVSEVGYFP
                     VKSARAAEK"
     gene            complement(1411..2646)
                     /locus_tag="AC16GM_06340"
     CDS             complement(1411..2646)
                     /locus_tag="AC16GM_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923329.1"
                     /translation="MKGRAMILAVTILLCLAPVGVRAQETASDSLKEKVTETADKVAG
                     IEERLGVDESDIGNLKKLKISGYVQARYEYHDDAVSTPALGTASTASSNQGKLLNRFF
                     IRRGRLKATYQATPNALGVVYFDGSASGVTLKEAYVVVSEPHSKVTATLGQFNWLFGY
                     EISYSSSKREFPERARWSRTLFPGERDRGVKVERPFLLAGKYTLTLTGGVYNGNGIED
                     AAFGVRDPNKAKDFLARAAVGLGHLDFGFSGYWGKQFNPSDSVIPKQQPSTTDKIRYG
                     ADAQFFYELPTLGGGVLKAEGVLAKEPKNQSKPFLSRDTTRDVAGFNVVWAQNLKEKF
                     QWISRVDFYDPNRDPDQDHFITYGLGLIYFWDGNSKVKLVYEIPKSYRNSTSGTVTVN
                     GVKIDEKDNLLTLEWVYTF"
     gene            complement(2811..>4045)
                     /locus_tag="AC16GM_06345"
     CDS             complement(2811..>4045)
                     /locus_tag="AC16GM_06345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR2923330.1"
                     /translation="FFLVVFATLSIAGAIFFLRQSRPVKQLVGHFERLKAGDFSARVS
                     VTSKDEFLLLGQTVNELAERLEQSFAELKEQKQVQDTTLHTLTEGVLAADGSGRIVFS
                     NPALRTIFHLEGGFIGQPFHTVFRQPGLLAAFDGVLKEGGIQKVTIKETFPTHRVIET
                     YFSSPRETRTVRLVAVFQDITQAYRLEQVRKDFVANASHELRTPLSVVKGYVETLEDG
                     LVSEEERKAVFQTLGRNIERMGLLIGDLLHLSKLESPEFTLNIGRVTLTEVARAALSS
                     LQSKIAAKKQKLTLDLPPDLSLRADRLELEVALKNLLENAIQYTSEGSAITLRAAEQN
                     GTVVVTVADTGIGIPSQELERIFERFYRGDKSRSREEGGTGLGLSIVKHIAEAHGGRV
                     EVESEVGKGSSFTLILPS"
BASE COUNT          831 a         1186 c         1113 g          915 t
ORIGIN      
        1 gcgactgtgg cattgaggcg catggtcagg tgaaaggcat cctgaatcca gctcgccagc
       61 acgattaagg caaaaaatcc gagcaccacc gaggggatac cggccaaaag ttccaccgca
      121 ggtttgatca tccgtcccaa gcggggcggg gcaaactccg aagtgaagat cgccgccagg
      181 attgccagag gggttgcgaa gaaaagcgcc acgaacgagg ccttgagcgt tcccagaaag
      241 agaggaatca agccgaactt cggttgatcc gagaccggct gccagattga cgtccacagc
      301 tggctgaact gaacttcctt tttcattgcc gggtcggtga ggagcggaaa ggcctccttg
      361 ccgatgaata tgaaaagaaa gacaattccc agaatcgaga cccaggaaga aagatagacc
      421 acaagctcaa cgcctcgctc gatgagcttg cgccggtcta tctttggcgg ggcgccgccc
      481 tttatgtgca aatttcgagc ggcgccatcc cgggttgcag gcattgttta cttttcagcc
      541 gcccgggcgg atttgaccgg gaaatacccc acctcgctca ccactttctg cccttccggg
      601 gacagcaccc aatcgaccaa tttcttgacg tcgccggttg gccggctgcg cagataccag
      661 tacaggtcgc gcgaaagagg atactggccg ctttccaccg tggccagatt cggctcgaac
      721 gcctgcccgt cggttttttc cgataccttg cagaatttga tccccttggc atacgcggct
      781 cccccgtacc cgatgccgtt tttatctttg gcgactgcgt tcaccaccgc cgccgtgccg
      841 ggcaaagtgg cggtctgggg agcgaaatcg gcacctttca aaacgttgtc cttgaagaaa
      901 acgtaggtgc cggagttgtt ctcccgtccg tacaggatga ttttggcatc cggcccgccc
      961 acctcattcc agttggtgat ttgcccggtg taaattgctt tcaactgctc cagcgaaagc
     1021 ttttcgaccg ggttggtttc gtgcaggtag acggccagcc cgtctttggc taccgggatt
     1081 tcaaccccta aggtgaagta gcgctctttc aacttcgtct tttccgcgtc tttcatcggg
     1141 cgggaggctt cacagatgtc ggtcgtgccg ttgatcagtg ccgcgatgcc ggtgccgctg
     1201 cccccgccgg tgacctgaat caccgaaccg ggatttttct gcatgtaaat ctcggcccag
     1261 cgctggccca atatcaccat cgtatccgag cccttgacgg tgatcgactt tcccgccaga
     1321 cctagccctg cgccgccgag gccgaatgcg agggctgccg ttaccagaaa acctctgtgt
     1381 ctcatctttc cctccttctt ttggttgcga ttagaaagtg tagacccatt ccagcgtcag
     1441 caggttgtcc ttctcgtcta ttttcactcc gttgacggtc acggtgcccg acgtactgtt
     1501 ccggtacgac ttgggaatct cgtacaccag tttcactttg ctgttgccgt cccagaagta
     1561 aatcaagccc aggccgtacg tgatgaaatg atcctggtcg gggtcgcggt tggggtcata
     1621 gaaatcgacc cgtgaaatcc actgaaactt ctctttcaaa ttctgcgccc agacgacgtt
     1681 gaaaccggcc acgtcgcggg tggtgtcacg cgagaggaac ggtttggact ggttcttcgg
     1741 ctctttggcc agaactccct cggccttcaa cactccgccg ccgagcgtgg gcagttcgta
     1801 gaagaactgc gcgtcggcgc catagcggat tttgtcggtg gtcgaaggct gctgtttggg
     1861 gatgaccgaa tcgctgggat tgaactgttt cccccagtag ccggaaaaac cgaagtccag
     1921 atgccccagc ccgacggcgg ctcgggcgag aaaatctttt gccttattgg gatcccgtac
     1981 cccgaaggct gcgtcttcaa tcccgttgcc gttgtagact ccgccagtaa gggtcagggt
     2041 gtactttccg gccaaaagaa acggccgctc cactttcacg ccccggtccc tctctcccgg
     2101 aaagagtgtg cgggaccagc gggcgcgctc cggaaactcc cgcttggatg aggagtagga
     2161 aatctcgtac ccgaagagcc agttgaactg ccccagggtg gcggtaactt tggaatgggg
     2221 ttcgcttacg accacgtagg cctctttgag cgtaaccccg gaggcggagc cgtcgaagta
     2281 gaccacgccc agcgcgttgg gagtcgcctg gtaggtcgcc ttgagccggc cccggcgaat
     2341 gaagaaccgg tttaacagtt tcccctgatt gctgctggca gttgaggcgg tccccagagc
     2401 cggcgtgctc acggcgtcgt cgtggtactc gtagcgggcc tgcacgtagc cggaaatttt
     2461 cagcttcttc aagttgccga tgtccgactc gtcgacacca agccgttcct cgatgccagc
     2521 caccttgtcc gccgtctcgg ttaccttctc ctttagactg tcgctggccg tctcctgcgc
     2581 cctgaccccg actggagcca agcacaagag gattgtgact gccaaaatca tcgctctccc
     2641 tttcacgcat ccctcctttt ttgatgaact agtcgaacca ttcgttcagg aggagatacg
     2701 ctttcattat ttccgacaaa tatcgggaat gttaaatcgg tgttaacagt tgccagcggt
     2761 ttcggagaca accgcctgat gaacgaaggt tttagtcttg aagagaaacg ttaagaagga
     2821 agaatcagag taaaacttga gcctttgccg acttcgcttt cgacttccac ccgcccgccg
     2881 tgggcttcgg caatgtgctt aacaatcgag agaccgaggc cggtgccccc ctcttcgcgg
     2941 gaacgggatt tatccccccg gtaaaaacgc tcaaagatcc gttccaactc ttgcgatgga
     3001 atgccgatgc ccgtatcggc gacggtgacc accaccgttc cgttctgttc agccgcccgg
     3061 agagtaatcg cgctcccctc tgaggtgtac tgaatcgcat tctccagaag gttcttaagg
     3121 gctacctcca gctccaaccg gtcggcccgg agggaaaggt cgggcggcag gtccaaggtc
     3181 aacttttgtt tttttgcggc gattttcgat tgcagactcg aaagcgcggc ccgggcaact
     3241 tcggtcaatg tcacccgccc gatgttcagg gtaaactccg gcgactccaa tttggagaga
     3301 tgcaggaggt cgccaatcag gagacccatc cgttcaatat ttctgccgag ggtttggaat
     3361 accgcttttc gctcctcttc cgagacgagg ccgtcctcca gcgtttcgac gtaccctttg
     3421 accaccgaga gcggagtacg gagttcgtgc gaggcgtttg cgacaaagtc tttacgcacc
     3481 tgctccagcc gataggcctg ggtgatgtcc tggaaaacgg ccaccagccg gacggttctg
     3541 gtctcccggg ggctcgagaa ataggtttcg attactcgat gcgtggggaa tgtttctttg
     3601 atagtaactt tctggatacc cccctctttc agtactccgt caaatgcagc caaaagaccg
     3661 ggctggcgaa aaaccgtatg gaacggctgg ccaatgaacc ccccttcgag gtgaaagatc
     3721 gtacgcagcg ccgggttgct gaaaacgatt cggccgctgc catccgccgc cagcactcct
     3781 tccgtcagtg tgtgcagtgt ggtatcctgc acctgctttt gctctttgag ttccgcaaag
     3841 ctttgctcca gccgctccgc caattcgttt acggtttgac cgaggagcag gaattcgtcc
     3901 ttgcttgtga cgctgacccg ggctgaaaag tcgcccgcct tcagccgctc aaaatgaccg
     3961 accaactgct tcaccggccg ggactgacgc aaaaagaaaa ttgctccggc aatggaaagt
     4021 gtcgcaaaga ccactaaaaa gaata
//
LOCUS       DBNGZD010000244        19163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1287865, whole genome shotgun sequence.
ACCESSION   DBNGZD010000244 DBNGZD010000000
VERSION     DBNGZD010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 19163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19163
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1287865"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..495)
                     /locus_tag="AC16GM_06350"
     CDS             complement(94..495)
                     /locus_tag="AC16GM_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR2923331.1"
                     /translation="MLTLTKKTDYGLLALHYLAQHNGGQRSKVREIARKYQVPEELLA
                     KVLQKLAKSGLVESAPGPAGGYRLKQKPKRITLAQVITAVEGPEHDLSCLRSESRGCN
                     RIDCCLTQRPTLKAQEKVAQVLEEMTLDDIS"
     gene            complement(599..1741)
                     /locus_tag="AC16GM_06355"
     CDS             complement(599..1741)
                     /locus_tag="AC16GM_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR2923332.1"
                     /translation="MRNQTVIPKVAPEKAKPQASRTARVVVLKTKPETVLDDYTRLLE
                     QAQSERLLDPRKDLILKLNLSWTKYFPACSTQPWQLEAVLRYLLAKGFDRNKLFPLEN
                     KTVVTKPVEGARLNRWLPVLKKFGLPFIPLPDQGWVKYDFRNELVHLKKLFPEGIEIP
                     ALYPGKQVLHLPTMKTHGHSVTTGAVKNAFGGLLKEVRHYAHKYIHEVLVELMVMQKE
                     LHPKVFAVMDGTVAGNGAGPRTMQPVEANFLLASADSVAIDAIAAKIMGFDPLSIPYL
                     RICHERGLGIADPKEIEVVGEDIRGVNLHFTVKKSLVIWGDQMLRIGPLRFLEKVALH
                     SPLVVWAPFASNFYHDWIWYPTVGKAIIRKFSRTGWGKLFRGYPKE"
     gene            complement(1901..2821)
                     /locus_tag="AC16GM_06360"
     CDS             complement(1901..2821)
                     /locus_tag="AC16GM_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923333.1"
                     /translation="MKLFSFLSERTQKVPAAGEAEPGGNAPTPGEGLPAHWHALAGRR
                     SLAEEKWKEAAQVAAAGIALNPDAYDLYELLLLAYRGSGRTEPKPKEVKKFLEQVSSG
                     PWRSILSGIKLVFDENFQEAWAELSPLPEHFPGSRLFPAIRALCAATLLTNHRPQLEH
                     PAQVRHRLQQDLSACRAADEHWSGAVKLFEEKNYRALLTAITRLFAERCTFPPLDAVE
                     KIHLEFLARPENFDFSEPVSSPTLAEDAPPGKFLAAELFSFLADCQKAEGEFASACQL
                     SPQFAKAQNNLTLIREQKAVLVSVLKDLRL"
     gene            complement(2818..3939)
                     /locus_tag="AC16GM_06365"
     CDS             complement(2818..3939)
                     /locus_tag="AC16GM_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923334.1"
                     /translation="MTTAAGPEAPAVLWEEFFRTGPVDVLAQTEYDETEGVFICQLFR
                     NRRLVGARRSVPVDPAGSFHDSGGAFHQREVLFLKTLFYLAKRLEKQGTATEETSVGQ
                     AFLVLGFFPEAEACFRWALERDPDSSEAHFRLAGVCHRLGKCAEAEALYRKALSLQPY
                     ADYANALGLLQLEGGKVKEAEEAFRQALEINPNYAEALLNLGHLYLEQAGSGKGRATD
                     ECFAAAEVYLSRAHLIAHAATSGADTKKEHFDRYEALCRIYLKTKNQLVAQHDSSNRR
                     ALCEVYLLALKYLAHELSAEQIGQFYFHLLDATNGKPDYPDLKNYAGLIVIFSGLVAL
                     EKAALEIVRQEKDERALELVLPIQQVLTATRTLKEKLAT"
     gene            complement(4092..4802)
                     /locus_tag="AC16GM_06370"
     CDS             complement(4092..4802)
                     /locus_tag="AC16GM_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR2923335.1"
                     /translation="MDDIRISLETAGPQNEVAIVRVDGVVDTLTASELEKVMNSLFEQ
                     KRFNIVVDLGGVDYISSAGWGIFISNIREIRRNAGDIKLARMVPSVYEIFELLEFDSI
                     LKAFDNVEKAKGDFGLDQKPKAAAPPAPSLPVSRPGAEAFRLPAGPAPVGSPARAGTS
                     VTIPVTRTPEPQAWVDAVLSLVGEDPFYSIAEIARELRSRFGYPGASWWQVFRLLASR
                     KLLRKKSRFRYQRMRSRN"
     gene            complement(4778..5596)
                     /locus_tag="AC16GM_06375"
     CDS             complement(4778..5596)
                     /locus_tag="AC16GM_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR2923336.1"
                     /translation="MKKIAVITSKGGTGKTTTAVNLAHGLALSGKKVLVIDCDSQGSV
                     AFAFGVQAERSLAELLVRGEVDFVRARPGLFVVDSGGKKLAEAELYLAARDDREVRLA
                     LALESFAGADYVLCDCSPSINLMNLNALFFCDSMLIPVSLDTFALKGVEKMLALAADV
                     RQAGHPIEPLGILATFYDGRTNIAKRLWNELLECYGEKVLATRIRVNTALKEAQAVQK
                     TIFEYSPYAHGAIDYFLLTREILAHPRLQPAPQMPGEQTADTAEPKVWMTSASL"
     gene            complement(5593..8460)
                     /locus_tag="AC16GM_06380"
     CDS             complement(5593..8460)
                     /locus_tag="AC16GM_06380"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR2923337.1"
                     /translation="MSIFQKPIKEIYATFPAEESRLEEVRTFVQTVTQDTGLASKDLT
                     GIQLAIEEACTNVIRHAYLYGPGTIRLKIVLGKDAIVFSIYDTGRQFNFGQAAGATPD
                     LKRYIATGRKGGLGLYLIQKIMDDVSYRSHNGTNEMRLTKKIPRKVPLSMRPVRGLSV
                     RVKFSLLSAAIVLAIMAAVFFFFDHRTTTAIYRADLANVEQLARTVAAAAEDPLLSGS
                     DLELMTVASGTRRQHPELSYLVVVDTGGVVLADPDRVEQVLTAYPLPPGLAPRRFWTP
                     QVVSPAGQETYHTVAPISIGGTFRGFVHVGLAKKTIEAEVVRARYVLILICLVGLLVG
                     FLAIWTLAQWFVKPIQKLTEGVLRIGQGELDATLPVEGKDEFGEIAKAFNDIALKFKQ
                     SQKTLLQQERLQKEIEVAQEIQRALLPRQFPEVEGYDIATIYRAAKDVGGDYYDFFWI
                     DEKTMGIVVADVSGKGVPGSLVMTMIRTAIRLEARGNRSPVEILSRVNAFVADDVKKG
                     MFITIFLVVLDSVNRTITYTSAGHNPMILYRKEEDRAYFLNPRGIPLGISLPDDLSFE
                     ETLAAETVRLKKDDILVIYTDGITEAMNAQKEQFGTQRLLDFIRANCPLAAEEFAEKF
                     NDELSTFTGDLPQHDDITLVAVKEQLVADKVVFATRKRLLDLVAVDGKTVREACAVIG
                     VAPTTYYRYKRLYDQFGPEGLKARTPRLGRGEEMRQVSIDDRQKILSVVALHPEYGPA
                     RVKSELASERFGHLTLDEEVIYDELVRLRLNTKLLRLEFAERTKGQLPATQAHLIDLE
                     RQKEKEQGEAERAAAERIAAAQRQEAAAQQAERQETFLTQLREQGLESDDLALLAMVA
                     GELKQVAEDEKVEKLFRLIGEKIVAEKKKKAGISGSPRPKVLDWQRLVTSHKPVTQKP
                     EAVTVPEEPGEEKPFNWEEYSRRVKARLVRSPRVPDEGKPE"
     gene            complement(8608..10539)
                     /locus_tag="AC16GM_06385"
     CDS             complement(8608..10539)
                     /locus_tag="AC16GM_06385"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2923338.1"
                     /translation="MKKPTPPLPSPQRGGKRKTFITSPWPSPARRGNVRIASSRPAKW
                     LFALALCWVSVGNAWGASDAGRRSLFSFGAGARAQGMGGAFVSVADDASAVFWNPAGL
                     LQLDQGKGLLQHATLFEGDFQFAGVAYPTLDLGTFGVGFMRIGTGDIPGRDRLGQPTG
                     TFSFAQSELVFAYGKKLFPYLGVGASVKAVAQELGDANDVGFGADVGLLLPRWRGLGL
                     GLTWQDAIGARLTLNQSREVMPWNLKGGLSYERFVAADRVGLLFSADLDQVQGGQARV
                     RVGGEVAIRRQLFLRAGLDKDEITFGAGVGWRSFAFDYAYQSVLDLEASHRFSLSIAF
                     GSSRAERLATHNKAAEEKALRVYQESQKEKVQNLFERAQTFDKAGAVDSALFFYQQVL
                     AYDAENARARERMAALKEQVKSQAEQAVQARSQELIVTDHLRLAEQYQGEGNLALAQA
                     ELQQVLAQSPQNPEALRRQKEIAGQIGRQKMAFDSTAEVFFTRKDYSGAIGEWRKALV
                     LNPGDTLFERKIKRAEERLSAAQLLQEGLAKYTAGEYSAALAIFGNVLRVDPQNPVAR
                     EYQKLSQQKMAKVTTLEDLKKDPQAWQWYLAGLESFQKGDYEKAIELWNQVLAVYPNN
                     QNALRNIEQAKKRLPKSGQ"
     gene            complement(10536..13298)
                     /locus_tag="AC16GM_06390"
     CDS             complement(10536..13298)
                     /locus_tag="AC16GM_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923339.1"
                     /translation="MYRGKFTFGLVVFLLILQHRSLVTAGTPSFLLSPAQDVSSNTLA
                     TLSTVTDTLNSGRVGVADTHLIGFTLTTQQVPFDGKIIISFPPEFDLSAVKSVFYSDT
                     DFAHPDPSVDSIIITGQEITVLLDSTGQPAALGARINLLIPAIRNATVAGFYSVTVTI
                     ADRQGRILDGPNVSQPFTLSPGPAITLVISPDTDLAVSAGSVIPFQARAQDQFGNPIP
                     ASAFTWALLFTPDSIGQLTGSSLLATKTGTGRVEVTSGTLKDTSGLITVTPAEAARFM
                     LTGTPDTVNAGQGWPGQVTVSVFDRFDNRKTDYVGAVYFLGTDVTDSVPYGPTNRYQF
                     LPADNGQKSFLGTQFKFFSAGSHILTVGDDLLTASSAPIQVLAGPLAAFAVQAPVDVA
                     AGQPVPLSLLNANDGFGNALSGQANVNFVLGGSLSPFGKSPVLTPIAYTNGTGSATQI
                     LVATEKVVLRVTASGISRQTDTIRVRPGTLDTLLFNLSSPQDIGATFSGTALVRALDI
                     CGNTVTDFNAVADSVVIMPDGAGGMANNVLKQPTDFSTGVANLTAKGTTYLGQGGLVR
                     FTAVSQSGKRGRSDFVVMQGLSVDTLRVFPKLVRRGQEIGVEVFLFNSSTVTAQVTAV
                     ELLSDRGLIGGLAPAGGYPVSIGFNASARVADTLILPFDFPPGVAYLGVRVRLTYNTT
                     PLTLQLQDLDSLTVAAPANLNYVVASVTPSLLTRGTDVTLSLQLLNSGTFQVLLDTNT
                     RLVLRHQNGDSLATHLAQATPVFGGGIAPAELQFAPVGVPLTFPRGYYRITSLQVEGS
                     ENQVAFAQSVPTNDSVLVESVVQTSEFTATPNPFSPHGGPVQFEYVLAQPSAVRLRIF
                     SLLGDLVFEQEYPAGTNGGQLGRNVILWNGTNQDGRFVANGVYVGLLTVEGSGETRKV
                     KVAVAK"
     gene            complement(13485..15221)
                     /locus_tag="AC16GM_06395"
     CDS             complement(13485..15221)
                     /locus_tag="AC16GM_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR2923340.1"
                     /translation="MSLRGRAISFSGIGLTAMALACAKRGLPEGGPVDTTPPKIVFTE
                     PADRAVRVDSLATIRITFSERMESRSVQSNLSITPELKTPPRLHWKGKILEIKPSLFW
                     EGERTYTFTFGGQAQDGHGIRMGETFTFSFSTNTVIDSGRIEGLVFAGLVPEVGASVC
                     AYRLNSTADIDPMRQKPEYVTVSGSSGKFRLSALADGRYRLFAVKDQNRDRLWQPKRE
                     PFAVGTGEITLDSNSRHQNGLQFFLAEQDTAAFGLVRCQNLGPFVVWLEFNQPLDFGA
                     WNWQENFELRERSDSAGRRSDVTKITRAAGMYEFDAEPKNLYLVTERLSIGSTYDLIL
                     KSFTDRYGKPLDSARAHCRFTVAAEPAPPLRIVQTIPRSGEKYFDPGQLPLLVVNYPL
                     KSPVDDAAVLLQDTTGIVVGHFRSGAMPVHLLFSPTNGRALSPGEVYRLRVDEKKIVD
                     ILGRRLGDSTRVIEFRTVAEESLGTVTGKVKASPRDGDVQVHLLFTGLTRLEVKRELT
                     MRATAAGNPYSVALLSGKYRLSGYVDANGNGRYDAGRVTPFLPAEPQTVLADTVNVRA
                     RFETEEVDLMFP"
     gene            complement(15218..16825)
                     /locus_tag="AC16GM_06400"
     CDS             complement(15218..16825)
                     /locus_tag="AC16GM_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923341.1"
                     /translation="MSRWVKGLAFSSIFVAGLFFLFGCAGPKATVKSGPLQKVRVRYD
                     PRAYNHFTNGEIAFRLGDYATAISEFQQALAYDPASDEIRESLARVLVHANQYAQAAT
                     TAESIANKTADVWLLLGESYRNLNQRSQAIEAYRKVTASDPENAQAWWYLSLLYHQEG
                     DLAKAEEAAGKVWRLRPNHYYLEQYLSILLEEKKIGAAITLLQKVIADDSTDYDAKLR
                     LAEVYENADSTQAALELYRQVLGRMANRKQGEYKIGQLYLGNDQFAQADSVFASLLAG
                     DSSNFGAAVYYAWCALAQNHYADAEGRFKRVVALSDSAYQGYSGLARVYLAQDSAAKA
                     VPLLRQGLGKAPEHPELNYFLGRAFAALKQYDSAVAAFRAAQAKEPNSQRVLFSLGSA
                     FERGGQFDSAVAVFEEFLKLYPDDPGANNYLGYMLAEKGIRLPDAKAMIAKALAKEPE
                     NGAYLDSYGWVLFRLGQLSDAESWLRKAVEKLDTLAVMRSDNAVIFDHLGDVLSLLGK
                     TDEAKKQWERALALDPNNEKIREKLAR"
     gene            complement(16948..17988)
                     /locus_tag="AC16GM_06405"
     CDS             complement(16948..17988)
                     /locus_tag="AC16GM_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019145.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923342.1"
                     /translation="MRRVVRFFVTALFLLVGVGVIRGQVTRPDSDPPQADKPVVPGKV
                     KIDAERQALLARFKETYNVKTLRLNENGVPEYIKGELSKGLKAGEPVEEAYEFFQVNR
                     EVFHIRNPRSELALVKVERGPTGWTHLFFKQVYDSITVYGGDLRVHFVTNRELQIING
                     SYYPGIEVSTVPEITAEEAQVRVIHDLPCKVQPPKIHSTTLLIYPVTNSVKSGATVYH
                     LAWAVSTSVVDSCGSWGYFIDAHSGEILHRGSNMRELGGPADAGKREDSSTTKTKEVK
                     AKRLNIIEISPGDGRDSHSTSPLWGNDVLINGDLADQKSESLAAAKNGDLYVVFEDYD
                     PTTKPDPSLPSP"
     gene            complement(18199..19026)
                     /locus_tag="AC16GM_06410"
     CDS             complement(18199..19026)
                     /locus_tag="AC16GM_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2923343.1"
                     /translation="MKRTLWLAVAALVILPVVALAADVGNRDVVKFATARIEPGYVIV
                     PVEIVNDEQLAAVDLPLKWSEGATLEKVVFTERASAFQNFVNIKNADRTVIIAMIAFQ
                     SSPTPAVTAGLVGGSGPVAELYFKLNTGVSKLSVDATTMKKPSHQVMLIYNNKVDKSV
                     SVETISPEFQPFSLASLGAGNVPKTFALGQNAPNPFNPSTRINYALPVAGNVRIEVFN
                     ILGQKVKVLADGYEEAGYKSVVWDGKDDQGSQVASGVYLYRINALNFSDIKKMTLLK"
BASE COUNT         3889 a         5952 c         5244 g         4078 t
ORIGIN      
        1 taactcaatt ttcagttggg gccgttcttt taccgccgct tctgccatgc tatctctcct
       61 tccattagct gttcacaaac ttgccgaaca ccgctatgag atatcgtcca gtgtcatctc
      121 ctccagcact tgggcgactt tctcctgcgc tttcaacgtc ggccgctggg tcaggcagca
      181 gtcgatgcgg ttgcacccgc ggctttcgct ccgcagacag gaaaggtcat gttccggtcc
      241 ttccactgcg gtaatgacct gggccagcgt gatccgcttc ggcttttgct tcagccggta
      301 gccgccggcc ggccccggcg ccgactccac cagcccgctt ttggccaatt tctgcaacac
      361 cttggccaat agctcctctg gcacctggta cttccgggcg atttcgcgga ccttcgaccg
      421 ctgcccaccg ttgtgctgcg ccaagtagtg cagcgccaga agcccgtagt cggtcttttt
      481 cgtcaacgtc agcattgttc atctccgacc gatttagtcg ccattataat acgtaggaat
      541 cctgccgttt gcaagcaccc gatcttggcg aactgccgat cttaacgctt cggggggatc
      601 actctttcgg atacccacga aacaactttc cccaccctgt ccggctgaac ttgcggatta
      661 tggctttccc gacagtgggg taccaaatcc aatcgtggta aaagttggag gcaaagggcg
      721 cccagactac taagggcgaa tgcagcgcca ctttttctaa gaagcggagc gggccgatcc
      781 gcagcatctg gtcgccccag atcaccagac ttttctttac cgtaaagtgc aggttgaccc
      841 cgcggatatc ttcgccaacg acttcgattt ccttcggatc agcaatcccc agtccccgct
      901 cgtggcagat ccgcaggtag gggattgaca gagggtcgaa tcccatgatc ttcgccgcaa
      961 tggcgtcgat ggccaccgaa tcggccgacg ccagaaggaa attggcctca accggctgca
     1021 tggtgcgcgg cccggcaccg ttgcccgcca ccgtgccgtc catgaccgca aagactttcg
     1081 gatgcagttc cttctgcatg accatcaact ccaccagcac ctcgtgaatg tacttgtggg
     1141 cgtagtgacg aacttctttc aataggcccc cgaaagcatt cttgaccgcg ccggtcgtga
     1201 ccgagtgccc gtgcgttttc atggtgggca ggtgcagcac ctgcttgccc gggtagaggg
     1261 ccggaatctc aatcccttcg ggaaaaagtt ttttgagatg gacgagctcg ttcctaaaat
     1321 cgtactttac ccacccctgg tcgggcaagg gaataaaggg tagaccgaac tttttgagca
     1381 ccggcagcca gcgattgagc cgcgctcctt ccaccggctt ggtgacgacg gtcttgtttt
     1441 ccagcgggaa cagtttgttc cggtcgaaac ccttcgccag caaatagcgc aacaccgctt
     1501 ccaactgcca cggctgcgtc gagcaggccg gaaagtattt ggtccaggag aggtttaatt
     1561 tgaggatgag gtctttccgg ggatcgagca ggcgctccga ttgcgcctgt tccagcagtc
     1621 gggtgtaatc gtccagcacc gtttccggct tcgtcttgag cacgaccacc cgagcggtcc
     1681 gcgacgcctg cggctttgcc ttttccggcg caaccttcgg tatgaccgtc tgatttctca
     1741 tagcaactga tcgtccggta aaatctctct tgttcaaaaa gctaaaatac ccaaaaacca
     1801 tactattggg tagcgccggt ctctttagcc tgagcgaacc ggaggcctga ccagcctgcc
     1861 cgccatttcg tttttagggg acaacctcgg gcgatttggc ttatagccgg agatccttga
     1921 gcaccgacac cagcactgct ttttgttccc gaatcaaagt gaggttgttc tgggctttgg
     1981 caaactgcgg cgagagctga caggcactgg caaactcccc ttcagctttt tgacagtctg
     2041 ccaggaaaga aaagagctcc gccgccagga atttccccgg cggagcatct tccgccagcg
     2101 tcggcgaact aaccggctcg ctgaaatcaa aattttcagg ccgcgccaaa aactccagat
     2161 gtattttttc gacggcatcg agcggaggga acgtacagcg ctcggcaaac aggcgggtaa
     2221 tggccgtgag cagggcgcga taatttttct cctcaaacag cttcaccgcc ccgctccaat
     2281 gctcatcggc ggcccgacaa gcggatagat cctgttgcag acggtgcctc acctgcgccg
     2341 ggtgttccag ctgcggccgg tggttggtca ggagcgtcgc tgcgcaaagg gctcgaatcg
     2401 ccggaaaaag ccgactgccg ggaaagtgct ccggcaaggg tgaaagctcc gcccaggctt
     2461 cctggaaatt ttcatcaaac accagcttaa ttccggaaag gatcgaccgc cagggacccg
     2521 aactgacctg ctctagaaat ttttttacct ctttgggctt cggttcggtc cggccgctcc
     2581 cccggtaggc caaaaggagt aattcgtaga ggtcatacgc gtcgggatta agggcaatgc
     2641 cagcggcggc aacctgagcc gcctctttcc atttctcttc ggctaacgac cgccggccgg
     2701 ctaaagcatg ccagtgcgcc ggcagacctt ctccgggggt aggagcgttc ccaccgggct
     2761 cggcctcccc ggccgcggga accttctgcg ttcgctcact caggaaggaa aataatttca
     2821 tgtcgccagc ttttccttga gcgttctcgt cgccgttaaa acctgctgaa tgggcagtac
     2881 cagttccagc gcccgttcat ctttctcctg ccgcacgatc tctaaggccg ctttttccag
     2941 cgccaccaaa ccggaaaaga tgacaatgag cccggcgtag ttcttcaggt cggggtaatc
     3001 gggtttcccg ttggtggcgt cgagcagatg aaaatagaac tggccaatct gctcggcgga
     3061 gagctcgtgc gcaaggtatt tgagggctaa gaggtagact tcgcacagtg cccgccggtt
     3121 ggacgaatcg tgctgggcta ccagctggtt tttggttttc aggtagattc ggcagagcgc
     3181 ttcgtagcgg tcgaaatgct ctttttttgt gtcggctcca gaggtggccg cgtgggcgat
     3241 gaggtgcgcc cggctgaggt agacttccgc cgccgcaaaa cattcatcgg tggctcgccc
     3301 cttccccgaa ccggcctgtt cgaggtagag gtgacccaag ttgagcaacg cctcggcgta
     3361 gttgggattg atttctaagg cctgccggaa ggcctcctcg gcttctttga ctttccctcc
     3421 ctccagctgg agcagaccta aggcgttggc atagtcagcg tacggctgca gggagagcgc
     3481 cttccggtac agcgcttcgg cttcggcgca tttgcccagc cggtggcaaa ccccggccag
     3541 gcgaaagtgg gcctcgctcg aatcgggatc gcgctccagc gcccagcgga aacaggcttc
     3601 ggcttccggg aaaaacccga ggaccagaaa cgcctgaccc acgctcgtct cttctgtcgc
     3661 cgtgccctgt ttctccaggc gcttggccag gtagaagagc gtcttcaaaa agagcacttc
     3721 gcgctgatgg aaagccccgc cgctgtcgtg aaaactgccg gccggatcca cgggaaccga
     3781 acggcgcgcc cccaccagcc gccggttgcg gaacagctgg cagatgaaca ccccttccgt
     3841 ttcgtcgtac tcggtttgtg ctaaaacatc caccggcccc gtgcggaaga attcctccca
     3901 caagaccgct ggtgcctcgg gaccggccgc tgtcgtcacc gtgacgctat tttccattcc
     3961 gctcgtcgct taaaagggac cgctggcacg gtcggtacga cccgtagggg cgtctgcgcc
     4021 gcaccgaccg tctccacagg gagacgaaaa gtttcaacca cgtcgaccgt taccgttccg
     4081 gatacgtccc gctaattccg ggaccgcatc cgctggtaac gaaatcggct ctttttacgc
     4141 aaaagtttac ggctggccag caggcggaac acctgccacc aggaagcgcc tgggtaccca
     4201 aatcgcgagc gaagctcgcg ggcaatttcg gcgatggaat agaagggatc ttctccgacc
     4261 agactgagca ccgcgtccac ccaggcctga ggttcgggag ttctggtcac cggaattgtc
     4321 acggaagttc ccgcgcgtgc tggactaccg accggggcgg ggcccgccgg cagacgaaac
     4381 gcttcggcac ccgggcggga aaccggaagc gaaggggctg gcggtgcagc cgctttcggt
     4441 ttctgatcca atccgaaatc gcctttggcc ttttcgacgt tatcgaacgc cttgaggatg
     4501 ctgtcgaatt ccaacagttc gaagatttcg tagaccgaag gcaccatccg cgccagtttg
     4561 atgtcacccg cgttgcgccg gatttcccgg atgttggaaa tgaaaatccc ccaccccgcc
     4621 gaggagatgt agtccacccc gcccaggtcg accacgatgt tgaaccgctt ctgctcgaaa
     4681 agcgaattca tcaccttctc caattcggaa gcggtaagcg tatccaccac cccgtccacc
     4741 cggacgatgg ccacctcgtt ctgggggccg gcggtctcta aagagatgcg gatgtcatcc
     4801 atacttttgg ctcagcggta tccgcggttt gctcccccgg catctgcggc gctggctgga
     4861 gccgcgggtg tgccagaatt tcccgggtca agaggaaata gtcaatggcc ccgtgcgcgt
     4921 agggactgta ttcgaagatg gtcttttgga ccgcctgcgc ctccttgagc gccgtattga
     4981 cccggattct ggtcgccagc actttttcgc cgtagcactc gagcaattcg ttccacaggc
     5041 gtttggcgat gttcgtccgg ccgtcgtaga aggtggccaa aatgcccaag ggctcgatgg
     5101 gatgccccgc ctgccgcacg tcggcggcca aagcgagcat cttttcgacc cctttcaggg
     5161 caaaggtgtc cagactcacc ggaatgagca tcgagtcgca gaaaaaaagg gcattcaggt
     5221 tcatcaagtt gatcgacggc gaacagtcac acaggacgta atccgctccg gcgaaggact
     5281 ccagcgccaa agccagccgc acctcccggt catcccgcgc ggctaagtac agttccgcct
     5341 cggccaattt cttgccgccc gagtcgacaa cgaagagacc gggccgcgcc cggacgaagt
     5401 ccacctcgcc tcttaccagt aattcggcca aagaacgctc cgcctgaacc ccaaaggcga
     5461 aggccacgct cccctgcgag tcgcagtcga tcaccaaaac cttttttccg gaaagggcca
     5521 gcccgtgcgc cagattgacg gcggtggtgg ttttgcccgt gcctcccttg gaggtgatga
     5581 cggcgatttt tttcattccg gctttccctc gtccggtacc cgcggcgacc gtacgagccg
     5641 cgccttcacc cgccggctgt actcctccca attgaacggt ttttcctctc ccggttcttc
     5701 gggtacggtc accgcttcgg gcttttgggt aaccggtttg tggctggtca ccagccgctg
     5761 ccaatccaac accttgggcc gcgggctgcc agaaatgccg gcctttttct tcttttcggc
     5821 cacaatcttt tcgcctatca agcggaagag cttttcgact ttttcatcct cggccacctg
     5881 cttgagttcg cccgcgacca tcgccagaag tgccagatcg tcagactcca gtccctgctc
     5941 tcggagctgg gtgagaaatg tttcctgccg ctcagcttgt tgcgccgccg cctcctgtcg
     6001 ctgggctgcc gcaatgcgct cagctgccgc ccgctcggcc tcgccctgtt ctttctcttt
     6061 ttgccgctcc aggtctataa ggtgagcctg ggttgctggg agttgtcctt tcgtccgctc
     6121 cgcgaactcc aaccgcaaca gtttcgtgtt cagccgcagg cggaccaatt cgtcgtaaat
     6181 cacctcttcg tccagcgtca gatggccaaa ccgttcgctg gccagctcgc tcttgacccg
     6241 ggccgggccg tactccgggt ggagggcaac gacggagaga atcttctgtc ggtcatctat
     6301 ggaaacctgc cgcatctcct cgccccgccc taggcggggc gttcgggctt tgaggccctc
     6361 cgggccgaac tggtcgtaca gtcttttgta acggtaatag gtagtcggcg ccacccctat
     6421 gacggcacag gcttcccgaa ctgttttccc gtcgacagcc accagatcga gcagacgctt
     6481 gcgcgtggcg aagaccacct tgtcggccac taattgctct ttcaccgcca ccagggtaat
     6541 gtcgtcgtgc tggggcagat cgccggtgaa ggtggaaagt tcgtcgttga acttctcggc
     6601 gaattcttcc gccgcgaggg ggcagttggc ccggatgaaa tccaaaaggc gttgggtccc
     6661 gaactgctct ttctgcgcat tcatcgcctc ggtgatcccg tcggtgtaga taaccaggat
     6721 gtcgtccttt ttcagccgca ccgtctcagc cgccagcgtt tcttcgaacg acagatcgtc
     6781 gggcagcgag atgcccaggg ggatgccgcg ggggttcaaa aaataagccc ggtcttcctc
     6841 cttgcggtag aggatcatcg ggttgtgccc ggccgaggtg taagtgatgg tccggttcac
     6901 cgagtcgagc accaccaaaa aaatggtgat gaacatccct tttttgacgt cgtcggccac
     6961 gaacgcgttc acccgggaga ggatttccac gggcgaccgg ttgccgcgcg cttccagccg
     7021 gatggcggtg cggatcatgg tcatgaccaa ggacccgggc acccctttgc ccgaaacgtc
     7081 ggccaccacg atccccatgg tcttctcgtc gatccagaag aagtcgtagt agtcgccccc
     7141 cacgtccttg gccgcccggt agatggtggc aatgtcgtac ccctccactt ccgggaactg
     7201 gcggggcaaa agcgcgcgct ggatctcctg cgccacctcg atttccttct gcagccgctc
     7261 ttgctgcaaa agcgtcttct gactctgctt gaatttcaac gcaatgtcgt tgaaggcctt
     7321 ggcgatttcg ccgaattcgt ctttcccttc caccggcagg gtggcatcta gttcgccctg
     7381 tccgatccgc aatactccct cggtcagttt ctgaatcggc ttgacaaacc actgcgctaa
     7441 agtccagatg gccagaaacc cgaccagaag ccccacgaga cagatcagaa tcaggacgta
     7501 acgcgctcgc acgacctccg cctcgatggt ctttttggcc aaaccgacat gcacgaagcc
     7561 ccgaaaggta ccgccgatgc tgatgggagc aaccgtatgg tacgtttctt ggcccgctgg
     7621 ggacacgacc tgcggcgtcc agaagcgccg cggcgccagg cccggaggca acgggtatgc
     7681 ggtcaggacc tgttccaccc gatcgggatc ggccaggacc accccgccgg tgtcgaccac
     7741 caccagatag gaaagctccg gatgctgccg tcgggtgccg gaggccaccg tcatcaattc
     7801 caaatcgctg cccgacaaga gcggatcttc cgccgccgct gccaccgtcc gggccaactg
     7861 ctccacgttc gccaggtcgg cacggtaaat ggcggtggtg gtccggtgat cgaagaaaaa
     7921 gaaaactgcg gccatgatgg cgagcacgat ggcggcggac agaagcgaaa acttcacccg
     7981 caccgacagt ccgcgcaccg gtctcatcga cagcggcacc ttcctgggta tttttttggt
     8041 cagccgcatc tcgttggtgc cgttgtgcga ccggtacgac acgtcgtcca taatcttctg
     8101 gatcaggtag agccccagcc cgcctttgcg cccggtggcg atgtagcgtt tcagatccgg
     8161 tgtcgccccc gccgcctggc caaaattgaa ctgccgcccg gtgtcataga tcgaaaagac
     8221 gatggcgtct ttgcccagga cgattttgag ccggatggtc cccggcccgt acaggtaggc
     8281 gtgccggatg acattggtgc aggcctcttc gatggccaat tgaatgccgg tgaggtcttt
     8341 ggaagccaga ccggtgtcct gcgtcaccgt ttggacgaag gtgcgcactt cctccaggcg
     8401 gctttcctct gccggaaaag tggcgtaaat ctctttgatc ggcttttgaa aaatgctcat
     8461 cgtgttttta ttccaacact ttgatggttt cgcttacttt gacccgtcaa aaaaatgagt
     8521 catccgcgtg gggcgacccc tacgggccgt atggccgcct cggtcgccct tcttcgatta
     8581 cctcactgga acaactcgcc gcctctctca ctgccccgac ttcggcaacc tttttttcgc
     8641 ctgctcaata ttgcgcagcg cattctgatt attgggataa acggccaaaa cctgattcca
     8701 caactcaatc gctttctcgt aatccccttt ctggaaactt tccagccccg ccagatacca
     8761 ttgccaggcc tgcggatctt tctttaagtc ctctaaggtg gtcactttgg ccatcttctg
     8821 ctgggagagc ttctggtatt ctctggcgac cgggttctgc ggatctaccc gcaagacgtt
     8881 cccgaagatt gccagagccg ctgagtactc cccggccgtg tacttcgcta agccctcctg
     8941 gagcaactgg gctgcggaca gccgctcctc cgcccgcttg attttccgct cgaaaagcgt
     9001 atcacccgga ttgagcacga gggctttgcg ccattcgccg attgcgccac tgtagtcttt
     9061 tcgggtgaaa aaaacttcgg cggtggaatc gaacgccatc ttctgcctgc caatctgtcc
     9121 cgctatttcc ttttgtcggc gcagggcttc gggattttga ggcgactggg ccagcacctg
     9181 ttggagctcc gcctgtgcca aggccaggtt cccctcaccc tggtactgct ctgccagccg
     9241 caggtggtca gtcactatca actcctgcga gcgggcctgt accgcctgct cggcctggga
     9301 tttgacctgt tccttgagcg ccgccatccg ttcgcgggct cgggcgttct ccgcgtcgta
     9361 cgccagaacc tgctggtaga agaagagggc cgagtccacc gcgccggcct tatcgaacgt
     9421 ctgtgctcgc tcgaagagat tttgcacttt ctctttttga ctctcctgat atacccgcag
     9481 ggctttttct tcggcggctt tattatgggt ggccaaacgt tctgctcggc tggaaccgaa
     9541 cgctatcgaa agggagaacc ggtgcgacgc ttctaagtcc aaaacactct ggtacgcgta
     9601 gtcgaaagcg aatgaccgcc agccgacgcc ggccccgaag gtgatttcat ctttgtccaa
     9661 gcccgcccgg aggaataatt gccggcggat cgccacttct cccccgacgc gcacccgcgc
     9721 ttgcccgccc tgcacctgat ccaggtcggc ggaaaagagc agaccgactc gatccgcagc
     9781 gacaaaacgc tcgtaactga gtccgccctt cagattccac ggcataactt cccgcgactg
     9841 gttcagggtc agtcgggcgc cgattgcatc ctgccaggtt aagccgagcc ccaatccccg
     9901 ccagcgcggg agaagtaaac cgacatcggc gccgaagccc acgtcatttg cgtcacccaa
     9961 ctcctgcgcc accgctttga cactggcgcc gacgcctaag tagggaaaaa gcttcttacc
    10021 gtaagcgaag acaagttcgc tctgcgcgaa agaaaaagtt ccggtgggct gacccaggcg
    10081 gtcccggccc gggatgtcgc ccgtcccgat ccgcataaag cccaccccga aagttcccag
    10141 atccaaagta gggtaggcaa ctccggcgaa ttgaaaatct ccttcgaaaa gcgtggcatg
    10201 ctgcaaaagt cctttaccct ggtccaattg caggagtccc gcgggattcc agaacaccgc
    10261 cgaggcatcg tcggctacgg aaacaaacgc cccgcccatc ccctgcgccc gcgccccggc
    10321 gccgaaggag aaaagcgacc gccgccctgc atccgaagct ccccaagcat taccgactga
    10381 tacccagcat aaagcgagag caaaaagcca cttcgctggg cgggaggaag caatgcgaac
    10441 gtttcctctc cttgcaggag agggccaggg tgaggttata aaggtctttc ttttccctcc
    10501 cctttgaggg gagggtaagg gaggggtcgg ctttttcact tcgccaccgc cactttcact
    10561 ttgcgcgttt cgccgctccc ttctaccgtg agcaaaccga catacacacc attggccacg
    10621 aaccgcccat cctgattggt gccgttccag agaatgacat tccgccccaa ttgcccacca
    10681 tttgtccccg ccgggtattc ctgctcgaac accagatcac ccagaagcga gaagattctc
    10741 aaccgaactg ccgagggttg cgctaggaca tactcgaact gcaccggccc tccgtggggc
    10801 gaaaagggat taggcgtagc ggtaaactcg ctcgtttgca ccaccgactc gactaagacc
    10861 gaatcgttgg tcggcacgct ctgggcaaac gccacctgat tttccgatcc ctccacctgc
    10921 agggacgtaa tgcggtagta accccgggga aaggttaggg gcacacccac tggtgcaaat
    10981 tgcaactccg caggggcgat ccccccaccg aagaccggcg tcgcctgcgc caggtgcgtg
    11041 gctaacgaat cgccattctg gtggcgcagc accagccggg tgtttgtgtc cagaagcacc
    11101 tggaaggtgc ctgaattcaa aagttgcagc gacagggtta cgtcggtgcc gcgggtcaaa
    11161 agagacggtg taaccgaagc cacgacgtaa ttcaaatttg ccggtgccgc cacggtgaga
    11221 ctgtccaagt cctgcaactg aagcgtgagc ggagtcgtgt tgtaggtcaa acgcacccgc
    11281 acgcccaggt acgccactcc gggagggaaa tcgaagggga ggatgagcgt atcggcgacg
    11341 cgggccgaag cgttgaaacc gatactcacc ggatacccgc ccgccggagc gaggcccccg
    11401 atgagcccgc ggtcgcttaa aagctccacc gccgtgacct gagccgtcac ggtggaagag
    11461 ttgaagagaa aaacttcaac gccaatctcc tgtccgcgcc gcaccagttt cgggaaaacc
    11521 cgcaaggtgt ccacactcaa accctgcatc acgacgaagt ccgaccggcc ccgcttcccc
    11581 gactggctga ccgcggtaaa ccgcaccagc ccgccctggc cgaggtacgt agtgcctttg
    11641 gccgtcagat tggccactcc ggttgaaaaa tcggtcggct gttttaagac gttatttgcc
    11701 attccaccgg ctccgtcggg catgatcacg acgctatcgg ctacagcgtt gaaatcggta
    11761 acggtattgc cgcaaatatc gagcgcgcgc accagcgccg tgccgctgaa agtcgcaccg
    11821 atatcctgcg gtgatgacag attaaagagc agtgtatcca gagtacccgg gcgcacccga
    11881 atcgtgtcgg tctggcggct gattccggag gccgttaccc gcagcacgac tttttccgtc
    11941 gccacgagaa tttgcgtcgc gctccccgtg ccgttggtgt aggcgatggg cgtgagtacc
    12001 gggcttttgc cgaaaggcga aagacttcca cccaaaacga agttgacgtt tgcctggcct
    12061 gagagagcat tgccgaaccc atcgttggcg ttcaaaagcg aaagcggcac gggctgtccg
    12121 gcagctacat caaccggtgc ttgtaccgca aatgcagcca gcggtccggc caaaacctga
    12181 atcggcgcac tggaagcagt taataagtcg tcccccaccg tgagaatgtg tgaccccgcg
    12241 gaaaaaaact tgaactgagt gccgagaaaa cttttctgtc cgttgtccgc cggcaaaaac
    12301 tggtaccggt tcgtcggccc gtagggcacc gaatcggtga cgtccgtacc cagaaagtat
    12361 accgctccca catagtcggt tttgcggtta tcaaatcggt cgaaaaccga caccgtcacc
    12421 tgtcccggcc agccctgtcc cgcattcacc gtatccggcg tccccgtgag catgaaacgc
    12481 gccgcctccg ccggagtgac cgtaatcaat ccgctcgtgt ctttaagagt accggaggtc
    12541 acttccaccc gcccggtccc ggttttcgtc gccagaagac tgcttccggt caattgaccg
    12601 atagaatccg gggtaaacaa gagtgcccag gtgaaagcgg atgcgggtat cgggttgcca
    12661 aattgatcct gcgctcgtgc ctggaacggt atcaccgacc ccgcgctcac ggccagatca
    12721 gtgtccggag aaatgaccag agttatggcc gggcccggcg agagggtaaa cggctgcgag
    12781 acgttggggc cgtccagaat tctcccttgc cggtcggcaa tggtaaccgt caccgaatag
    12841 aatccggcca cggtcgcgtt ccgaatcgcc gggataagca gattgatccg cgcccccaaa
    12901 gccgccggct gcccggtact gtccagaagc accgtgatct cttgaccggt gataatgatg
    12961 ctatcgacgg aaggatccgg atgagcaaag tcggtgtcgg agtagaaaac acttttgacc
    13021 gcggagagat caaactccgg cggaaacgaa atgatgattt tgccgtcaaa cggcacctgc
    13081 tgggtggtga gagtgaaacc gatcaggtgg gtatcggcca cccccacgcg gccggagttg
    13141 agcgtgtcgg taacggtgga aagtgtcgcc agagtgtttg aggatacatc ttgggcgggg
    13201 gaaagcaaaa atgacggcgt tcccgccgtc accagtgacc ggtgctgaag aatgaggaga
    13261 aaaactacca gaccaaaagt gaacttacct cggtacatcc tccctcaagg ttcgtcccgc
    13321 cattggcggg gccactgacc taatcgccat ccgtggcacc ggcaccagcg agattcccgc
    13381 ctcgctcaat tccgccatct ccctaaagag agtataatca gccacgcgga aaaccgaaag
    13441 aaatttttga gtttgctcac caaaaatcgc cacagctgct tctttcatgg aaacattaag
    13501 tccacttcct ccgtttcaaa ccgcgcccgc acgttcaccg tatcggccag taccgtctgc
    13561 ggctccgccg gcaggaatgg ggtcacgcgc cctgcatcgt accgcccgtt cccgttggca
    13621 tccacgtagc cggacagccg atacttcccg gatagcaggg cgaccgagta cggatttccc
    13681 gcagcggtag cccgcatggt taactcccgt ttcacctcca ggcgcgtgag acctgtgaaa
    13741 aggaggtgga cctgaacgtc tccgtctctc ggcgaagcct ttacctttcc tgtgaccgtc
    13801 cccagcgact cctcggccac ggtgcggaac tcgatcacgc gcgtcgaatc ccccagccgc
    13861 cggcccagga tgtctacaat tttcttttcg tctacgcgta aacggtatac ctcccccggc
    13921 gagagcgccc gcccgttggt cggggaaaac aaaaggtgca ccggcatcgc cccgctccga
    13981 aaatgcccca cgactattcc cgtggtgtct tggaggagca ccgccgcatc gtccacggga
    14041 cttttcaaag gatagttcac gaccaaaagc ggcagttgtc ccggatcaaa atacttttcg
    14101 ccgcttctgg gaatggtttg gacaatacgc aggggcggtg ccggttcagc cgccaccgtg
    14161 aaccggcagt gcgctctggc tgaatccagc ggcttcccgt agcggtcagt gaaactcttc
    14221 aaaataaggt cgtaggtgga accgatggac aggcgctccg tcactaaata gagatttttc
    14281 ggttcggcgt cgaattcata catccctgcg gcgcgagtga tctttgtcac atctgaccgg
    14341 cggcctgcgg aatcggagcg ctcccgcagt tcaaaattct cctgccagtt ccaggcccca
    14401 aaatcgagcg gctggttgaa ctccagccac accacgaagg gccctaagtt ctggcaccgc
    14461 accagaccga aagccgccgt atcctgctcc gccaggaaga actgtaagcc gttctggtgt
    14521 cgggaatttg aatcgagggt gatctctccg gtgccgacgg caaagggctc ccgtttcggc
    14581 tgccacagcc gatcgcggtt ctggtctttg accgcgaaca ggcgataccg gccgtccgcc
    14641 aaagcggaaa ggcgaaactt cccggaagag ccgctcaccg tcacatactc gggcttttgg
    14701 cgcatcggat caatgtccgc ggtgctgttt agccggtatg cacacacgct ggctcccact
    14761 tccgggacca agccggcgaa aaccaaccct tcaatcctgc ccgagtcaat caccgtattg
    14821 gtggaaaagg agaaagtaaa tgtctccccc atccggatgc cgtgcccgtc ttgcgcctga
    14881 cccccgaagg taaacgtgta cgtccgttct ccctcccaga agagagacgg cttgatctcc
    14941 agaatcttcc ccttccagtg cagccgtggg ggagttttta gttccggagt aatggagaga
    15001 ttggactgga ccgagcggga ctccatccgt tcgctgaaag tgattcgtat cgtcgccagc
    15061 gaatcaaccc ggacggcccg gtcagccggc tccgtgaaga caatcttggg cggcgtggta
    15121 tcgaccgggc cgccttccgg caatccccgc ttggcgcaag cgagcgccat tgcggtcagg
    15181 ccgattccgg agaaagaaat cgcccgcccc cgcaaactca ccgagccagt ttctccctga
    15241 tcttttcatt attgggatcc agcgccagcg cccgctccca ctgctttttg gcttcatcgg
    15301 ttttgcctaa aagcgaaagc acgtcaccca ggtggtcaaa gatcaccgca ttgtcgctcc
    15361 gcatgactgc cagtgtgtct aacttctcca ccgccttgcg cagccaggat tccgcatcgg
    15421 agagctgtcc caggcggaag agcacccatc cgtaactatc caggtaggcg ccattttccg
    15481 gctctttggc cagggccttg gcgatcatcg ccttggcatc cggaagccgg atccctttct
    15541 ccgccagcat gtatcctaag taattgttcg ccccgggatc atcggggtag agtttcaaga
    15601 actcctcgaa gaccgccacc gccgaatcga actgcccccc ccgttcaaaa gccgatccga
    15661 gagaaaacaa cacccgctgg ctgtttggtt ctttggcttg agccgcgcgg aaggccgcca
    15721 ccgccgagtc gtactgtttg agcgcggcaa acgcccggcc cagaaagtaa ttgagttccg
    15781 gatgctccgg cgctttgcct aatccctgac gcaggagggg caccgctttg gccgccgaat
    15841 cttgtgctaa atagacccgc gccagcccgc tgtacccctg gtaggccgaa tcggacagcg
    15901 ccaccacgcg cttgaaccgc ccctccgcgt cggcatagtg gttctgcgcc agcgcgcacc
    15961 aagcatagta gaccgccgcg ccgaaattgc tggaatcgcc ggccagaaga gaggcaaaga
    16021 cgctgtcggc ctgggcgaac tggtcgttgc cgaggtagag ctgcccgatt ttgtactccc
    16081 cctgcttccg gttcgccatc cggcccagga cctgccggta gagctccagc gccgcctgag
    16141 tgctgtctgc attctcgtag acctcggcca ggcgcagctt cgcatcgtaa tcggtggaat
    16201 cgtcggcgat gaccttctgg aggagagtaa tggccgcccc aatttttttt tcttccagca
    16261 aaatcgagag gtactgttcc aagtagtagt gattcggccg cagacgccag accttccccg
    16321 ccgcttcttc cgctttggcc agatcaccct cctggtgata gaggagcgac agataccacc
    16381 aggcctgggc attttccgga tcggacgccg tgaccttccg gtaggcctcg atggcttggc
    16441 tccgctggtt taagttgcgg taggactccc ccaaaaggag ccagacatcg gccgtcttgt
    16501 tggcaatcga ttccgccgtg gtcgccgcct gcgcatactg gttggcgtgc accaaaaccc
    16561 gcgccagcga ttcccggatt tcgtcggagg cgggatcgta ggccagcgcc tgctgaaatt
    16621 cagaaatggc ggtggcgtaa tcgcccagcc ggaaggcgat ctcgccgttg gtgaagtgat
    16681 tgtacgcccg cgggtcgtag cggaccctga ccttctgtaa cggcccgctt ttgactgttg
    16741 ccttcggtcc ggcgcagccg aaaagaaaga ataatcctgc aacaaaaatt gaggaaaacg
    16801 caagcccttt gacccaacgc gacatccgaa gtcaatttac gcctctttgc gaactatttc
    16861 aagtcgcaga ccccgccaca ggcggggtag aaagcatttc ccacattcac taaatactac
    16921 ggtcgtgaac ttttttcctc cccttactta aggggagggt agggaggggt caggtttcgt
    16981 cgtcggatca taatcctcaa aaacgacata aaggtcccca tttttggccg ccgccagcga
    17041 ctcgctcttt tgatcggcca aatctccatt tatgagaacg tcattgcccc agagcggtga
    17101 agttgaatgt gaatcccgac catcgcctgg agatatttcg attatgttca atcttttcgc
    17161 cttgacttct ttagtcttcg tggtgcttga gtcttcccgt ttaccagcat cagcagggcc
    17221 acctaactct ctcatattac ttcccctatg cagaatctct ccgctgtggg catctatgaa
    17281 gtacccccag gatccacaag aatcgaccac ggaagtgctc accgcccacg ccaaatggta
    17341 aaccgtggcc ccagatttga cggaatttgt caccggataa ataagcagtg tcgtagaatg
    17401 gatttttggc ggctggactt tgcagggcag atcatgaatc acccgcacct gtgcttcttc
    17461 cgcagtaatc tccggcaccg tcgaaacctc aatgcccgga tagtacgatc cattaataat
    17521 ttgaagttct ctattagtga cgaagtgaac acgcaagtcc cccccataca ctgtgatgct
    17581 atcgtaaact tgtttaaaaa aaagatgggt ccaccctgtt ggaccccgtt caactttgac
    17641 taacgcaagt tccgagcggg gattacgtat atgaaaaacc tcccgattta cttgaaaaaa
    17701 ttcatatgcc tcttcgactg gttcacccgc tttaagccct tttgatagtt cgccctttat
    17761 gtactctggc accccgttct cattcagccg caacgtctta acgttataag tctccttaaa
    17821 acgggccagg agggcctggc gctcggcatc tatttttact ttgccgggaa caacgggctt
    17881 atccgcctga ggcggatccg aatccggccg ggttacttgt cctctgataa ccccaacccc
    17941 cacaaggaga aacagtgcgg ttacgaaaaa tcgaacgact cttctcatcg ccagtcttgt
    18001 tgtacatagt taacgtagtc tgtagctcca acttctgtca agtttttctc ggtagaccgg
    18061 tcggtacgac ccgtatgggc tcgtctccga gccagtctcg gtgtccatct gctaatctgc
    18121 ttcatcagct gtgaagaacc atccccaaac aaaaaccccg ccaaaggcgg ggcttttgag
    18181 actttcctgg ctgaagattt acttcagcaa ggtcatcttc ttgatgtcgg agaagttcaa
    18241 ggcgttgatc cggtagaggt agacgcccga ggcaacttgg ctgccctggt cgtccttgcc
    18301 gtcccagacc accgacttgt acccggcctc ttcgtagccg tcggccagaa ccttcacctt
    18361 ttggcccaga atgttgaaca cctcaatccg cacgttcccg gccaccggca gagcgtagtt
    18421 aatccgggtg gaggggttga acgggttggg cgcattctgt cccaaagcga aggtcttggg
    18481 cacattgccc gctcccagag aggcgagcga gaacggctgg aactccggcg aaatcgtctc
    18541 cacggagacc gacttgtcga ccttgttgtt gtaaatcaac atcacctggt gcgacggttt
    18601 cttcatggtc gtcgcgtcca ccgacaactt cgatacgccg gtgttcaatt tgaagtagag
    18661 ctcggccacc ggtccagaac ctcccacgag tccggcggtc accgccggcg tcgggctgct
    18721 ctggaaggcg atcatcgcaa tgatgaccgt gcggtccgcg ttcttgatgt tgacgaagtt
    18781 ctggaacgcg ctcgcccgtt cggtgaagac caccttctcc agcgtggccc cttccgacca
    18841 cttcaggggc aggtccaccg ccgccagttg ctcgtcgttc acgatttcca ccggcacaat
    18901 gacgtagcca ggttcgattc gcgcggtcgc gaatttgact acgtccctgt tgccgacgtc
    18961 cgccgccagt gctacaaccg gaagaatcac cagcgcggct acagccaacc acagtgtacg
    19021 cttcatcagc atacctcctg taaaagtttt actgcaaatt ccctgccggc gcttttggga
    19081 cggtgccgca accataccaa aatcgcccgc ttcgcttctc tactttcgca aaacacgcac
    19141 caggtattgc cgttctcttt cgg
//
LOCUS       DBNGZD010000245         7870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_661928, whole genome shotgun sequence.
ACCESSION   DBNGZD010000245 DBNGZD010000000
VERSION     DBNGZD010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_661928"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="AC16GM_06415"
     CDS             <1..903
                     /locus_tag="AC16GM_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923344.1"
                     /translation="EDRQIKWADFQTPDGRAYQRPVAAYSDPVRKTEHYLAPFPEGNK
                     LVRVQDLMIEQIVYSNINQGWRYPVYFSRTVADTKDRAGLDPHLQAEAAVWRIVPQQG
                     TNMADIVRADSFLTQVYRYRRWSDYRVTKDWNAASLVMMFPEMLIDLSTTYQGKGDTT
                     RAVMLLEKTVATFPDYWRPYVFLGMYYTAHGRKAKADSLYAAGEVYLRKVLRRDAGNP
                     LYYQFLAGLLHSSGKKDQAYAVLEEARQQLPESNFIFQTLLTLYYQDNRIKELVALAR
                     DWLGRNPEDRTATQVIQQFGPQVK"
     gene            1381..2679
                     /gene="dinB"
                     /locus_tag="AC16GM_06420"
     CDS             1381..2679
                     /gene="dinB"
                     /locus_tag="AC16GM_06420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR2923345.1"
                     /translation="MPEPFVVSPAVGGTEPTILHVDIDAFFAQVEQVLNPKLRGKPVA
                     VGGYPGERGVVACPSYEARKLGLKTAMPLSQAYRIAPQTIFIKGEYAVYQQFSQRFYE
                     ILLHFTPKVEMLSQDEAAADVGGSLRRFGTTRKLAETLKQEIKQELGLTTSVGAATNR
                     YLAKIACEFEKPDGLTIVMPGEEENFLRPLSVEVIPGVGSRTQAALGRLGIHTIGDLL
                     RIPPKSLERLFGKRGREIAAIASPGNRPHYGVDFDIEPEEEQKSISRSTSFLANSSDP
                     TFLLATLYYLLERACLALRRLGKLAWTVSVTLRFADYTFNHQAKRLSRPLSAEALIFP
                     EVKQIFQTLYSGSQPLSLVGVGLTNLIPCARQLSLFSDPACGEQGRTTERVLRLTHAL
                     DAVRTRFGYHTLISGRTFPLRKTYSESSRGFILRTPSLSR"
     gene            complement(2724..3590)
                     /locus_tag="AC16GM_06425"
     CDS             complement(2724..3590)
                     /locus_tag="AC16GM_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HIR2923346.1"
                     /translation="MQQKRRRNDPCYCGSGKKFKHCHSAQQQVDNLRFSGIAGAFLDQ
                     ENRLFIMSESVVVNQLRKEAPKIAEGFDKQFSDQLNQISVEVSRAMSILFFGAVGPTR
                     LSKVQETCLPLLLNASDSSVAALELLRNGHRLQPGILIRNVLESLCAALYLFQLPDEI
                     PLFMKGKLDSAKTVGKAKEAVPILGRLYGYFSNEFAHLGVSHRSFNPLIPFEKKDNSA
                     IVNLAFIRMSVALLYITAELIFLDSISRPRYWRKVATKVYSFSPSEKEWDWLINFLGV
                     SIDKTESALRTS"
     gene            complement(3732..5852)
                     /locus_tag="AC16GM_06430"
     CDS             complement(3732..5852)
                     /locus_tag="AC16GM_06430"
                     /EC_number="3.4.14.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR2923347.1"
                     /translation="MKDGVRRSHLGMFLVLTFGVVAFTGGGSLLFADEGMWTFDNPPL
                     KQLQERYRFTPTQEWFDHVRLSSVRFNDGGSGSFVSPSGLVLTNHHVALGQLQKVSSP
                     GKDYVKEGFYAKTQAEEMRCPDLELNVLISMKDVTERVQGVVKKRMSDKEALEARKAE
                     RAKIEKESLDSTGLRSDVISFYQGGEYWLYRYKKYTDIRLVFAPEQQIAFYGGDPDNF
                     TYPRYDLDMALFRVYENDKPVESRHYLKWNAKGAAEGELVFVSGHPGSTSRLRTLAQI
                     EGSRDYIYPLLIQTLKRRLEVLKRYAERGPEPAREAAGQIFGIENSLKALAGEYNGLL
                     DKNLMAKKKREEEDFRALVASNPDWKKEYGRAWEAIAKAEKKQQKTLTQRWFRGLRGS
                     RLPDLALTIVRYVTEVTKPDGKRFQEYRDSQLESMRFRLFSPAPIYPQLEEALLTHGF
                     QESLDALGPDDPFIKALLQGRAPAEVAKEVITRTKLADPDFRRSLVDGGEAAVAAATD
                     PLIVLARRVDPLLREERKWYEDNIESVETAAGEKIGTARFTVYGKSVYPDATFTLRLA
                     YGTVKGYPMNGTQAPSKTTLYGLYDRAKSFDLRSPYDLPARYVEGTDKLDLATPLNFV
                     STCDIIGGNSGSPVINRNAEFVGVIFDGNIESLIGDLVYHEENNRAVAVHSAAMIEAL
                     RKLYDAAPLADELEGKAYGASGSE"
     gene            complement(5946..6986)
                     /gene="lpxD"
                     /locus_tag="AC16GM_06435"
     CDS             complement(5946..6986)
                     /gene="lpxD"
                     /locus_tag="AC16GM_06435"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR2923348.1"
                     /translation="MPVTLGEIAQKTGGTLLGDPAVAITGVAGIETAGPGQLTFVANQ
                     KYRSLLVGTQATAVIISPDVATDSIKTALIQHPNPYYAFLVALRLFHPQERLYPPGIH
                     STAVVGKNVTLAEGVHLGPCVVLAEGVSVGEHSALLSGTYVGRNSKIGNDCLIYPNVT
                     AREGVTIGDRVIVHSGTVVGSDGFGFAKEGGVYHKIPQVGGVVVEDDVEIGANTTIDR
                     ATMGNTIIGRGTKIDNLVQIAHNVAIGENSIIVAQVGISGSTKIGKNVTLAGQVGLVG
                     HLTIGDNVIVAAKSGIHRDLKPGTVYFGYPARELSKQKRIEAVISRLPELAERVKALE
                     KKVKELTGQVQK"
     gene            complement(6990..7517)
                     /locus_tag="AC16GM_06440"
     CDS             complement(6990..7517)
                     /locus_tag="AC16GM_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR2923349.1"
                     /translation="MSARQMLAVAAALVGLTAGVAFGQEGKIGFIDSNRLRNELKEFK
                     DAQAKLDKEAVDWKKHADSLQQAITTSETELERQRIFLSDEKRKEKESEIGLKKQELV
                     RYNTEIYDPVAGKYYRRNVELTKPILDRINQALERLATENNYVLIFDAVNGSIAYGRK
                     GLDLTDKLLQELAGR"
     gene            complement(7672..>7870)
                     /locus_tag="AC16GM_06445"
     CDS             complement(7672..>7870)
                     /locus_tag="AC16GM_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923350.1"
                     /translation="DAGNAWASGREVTPFAHLFKSAGFGIRLTVPGIGLIGFDFAYGF
                     DQSRFVQGRRWRPHFQVGTTF"
BASE COUNT         1642 a         2383 c         2045 g         1800 t
ORIGIN      
        1 gaggaccggc aaatcaaatg ggccgatttc cagactcccg acggtcgagc ctaccaaagg
       61 ccggtggcgg cctattccga cccggtgcgg aagaccgaac actatctcgc tcccttcccg
      121 gagggcaata agcttgttcg ggtacaggac ttgatgatcg aacagatagt ctacagcaac
      181 attaaccaag gctggcgcta tccggtctac ttttcccgca ccgtggccga taccaaagac
      241 cgcgccggtc tcgaccctca cctgcaggct gaagctgccg tctggcggat cgtgccccag
      301 caggggacga atatggccga tatcgtacgc gccgattcct tcctgacgca ggtctaccgc
      361 taccggcgct ggtccgacta tcgggtcacc aaggactgga acgccgccag tttggtcatg
      421 atgttcccgg aaatgctgat cgatctctcc accacgtacc agggcaaagg cgacacgacc
      481 cgggcggtca tgctgctcga aaaaaccgtg gccacttttc ccgactactg gcggccttac
      541 gtttttctgg gaatgtacta caccgcccac ggccggaagg ccaaagccga ttccctctac
      601 gccgccggtg aagtttacct gcggaaggtg ctccgccgtg acgcgggaaa tcctctctat
      661 tatcaattct tagctggcct cctccatagt tccggaaaaa aggaccaggc ctacgccgtt
      721 ttggaggaag cccgccagca gttgccggag tcgaacttca tcttccagac tctgctaact
      781 ctgtactacc aggataaccg catcaaagaa ttagtggcct tagcccgcga ctggctgggg
      841 cgcaatccgg aagaccgcac cgccactcag gttatccagc agttcggccc gcaagttaaa
      901 taggaacttc ttcacacaga agaatctctg taggggcgaa tctttacttg tcctgagcga
      961 agccgaagga gattcgcccg tttttctcgg tggatgttgg atttcaagaa ccggccttaa
     1021 ggccggttct tttttttaca acctcttttc gccgcgcgcg gcagatgtaa acatctgccg
     1081 ccgctcctca aaagtattcc gtagccgagg gttttattct cagccccgcc ttcggcgggg
     1141 tggcattaca aagaggcaac ggctcggtag ccgccgaggt tagaaacctc ggctacggct
     1201 ttggaatcgc cagacccgac cttacttgtg gtgagcgagt tgtcttcttc attctcgcgc
     1261 aggcgggtcg gaaccccgcg taggcggggc tcttagcccc gccttctctc tcccttgact
     1321 actgctcaaa cgtacagtat atttctcccg tagcatctga cgccgaaaga gcggatcttt
     1381 atgcccgaac catttgtggt gagtcccgcc gttggcggga ccgaaccaac cattctgcac
     1441 gtcgacatcg acgccttctt cgctcaggtg gagcaggtgc tgaacccgaa actgcggggg
     1501 aaaccggtgg cggtgggcgg ctatcccggc gagcgcgggg tggtggcctg ccccagttac
     1561 gaagcgcgga agttgggcct caaaaccgcc atgcccctct cccaggccta tcgcattgct
     1621 ccgcagacga tcttcatcaa aggcgagtat gccgtttacc agcagttttc ccagaggttc
     1681 tacgagattc tcctgcactt cacgccgaaa gtcgaaatgc tctcccagga cgaagccgcc
     1741 gccgatgtcg gtggctccct ccggcgtttt gggacaaccc gcaagttagc ggaaacgctc
     1801 aagcaagaga taaagcagga actgggactg accacttccg tcggtgcggc gaccaaccgc
     1861 tacttagcca agattgcctg tgaattcgag aaaccggacg ggctgacgat tgtaatgccc
     1921 ggagaagaag aaaatttctt acgccccctc tcagtggaag tcatccccgg cgtcggctcg
     1981 cgcactcagg ccgcattggg tcgtctgggg attcacacga taggcgacct gctccggata
     2041 ccgcccaaaa gtttggagcg gctcttcggc aagcgtggcc gggagattgc agcaattgct
     2101 tcgcccggca acagacctca ctatggagtg gattttgata ttgagccgga ggaagagcag
     2161 aagtcaattt cccgcagcac cagttttctg gccaattctt ccgaccccac cttccttctg
     2221 gcgacgctct attacctgct ggagcgggcc tgcctcgccc tgcgccgatt ggggaagttg
     2281 gcctggacgg tttcggtgac tctccgcttt gccgactaca ccttcaacca tcaggccaaa
     2341 cggctctcgc gtcccctctc cgccgaagcc ctgatctttc cggaggtcaa gcaaatcttt
     2401 cagacactat acagtggcag ccaacccctt tcgctggtgg gagtaggatt gacgaacctc
     2461 atcccctgcg cccgccaact ctcgctcttt tccgaccctg cttgtggtga gcaaggtcga
     2521 accacggagc gagtcctgcg cctcacccac gcgttagacg ccgtccgcac ccgctttggc
     2581 taccatacac tgatttcagg ccgcaccttt cctctgagaa aaacttatag cgagagttcg
     2641 cgtgggttta tcctgcgtac gccgtcgctc tcacggtaac gaccacggcc cactctgatc
     2701 actgcacccc atctcacgta tatttatgaa gttcgtagcg cgctttccgt tttatcaatg
     2761 gagacaccga ggaaatttat tagccaatcc cattctttct cgctcgggct aaacgagtaa
     2821 acctttgtag ctacctttcg ccagtatctt ggcctcgaga ttgaatccag gaaaataagt
     2881 tctgctgtaa tgtataagag agctacgctc atccgaatga aagcaagatt tacgatcgct
     2941 gagttatctt ttttctcgaa aggaatcaac ggattaaaag atctatggga aacgcccaag
     3001 tgtgcaaact cgttgctaaa gtaaccgtag aggcgaccta atatcggaac cgcttctttc
     3061 gcttttccaa cggtcttcgc tgagtcgagt ttgcctttca taaaaagtgg aatttcgtca
     3121 gggagttgaa agagatacaa tgctgcacag agagattcaa gaacattgcg aatcaatatg
     3181 cccggctgaa gccgatgacc atttcgaagc aactcaagag cggcaacaga tgaatcactt
     3241 gcgttcaata aaaggggaag acacgtttct tgcactttag ataaacgggt agggccaacc
     3301 gcaccaaaaa aaagaattga cattgctcga ctcacctcta ccgatatttg gttcaactgg
     3361 tctgaaaatt gtttatcaaa accttcagca attttaggag cctccttgcg cagctggttg
     3421 acgacgactg actcactcat gataaacaat ctattttctt gatctaggaa agccccagct
     3481 attccagaaa atcttaagtt atcaacctgt tgctgcgcac tgtggcaatg cttaaacttt
     3541 ttgccactac cacaataaca agggtcgtta cgacgacgtt tctgctgcac gtatctagct
     3601 ttgtttactc caaaatttcc taaccctgct tatttactcg ccctactaac ccctcgatcc
     3661 tcttctccag ctccttcacc ctctccgcca actccggcag gcgagaaatc accgcctctc
     3721 acctccggca tttattccga cccgctagcg ccgtacgctt tcccctccaa ttcatccgcc
     3781 agcggagcgg cgtcgtacaa tttgcgcaag gcctcgatca tcgcggcgct atgcacggcc
     3841 acggcgcggt tattctcttc gtggtagacc aggtcgccaa tcaaagattc gatgttgccg
     3901 tcaaaaatca cccccacgaa ctcagcattc cggttgatca ccggcgaccc ggaattgccg
     3961 ccgatgatgt cacaggtgct gacgaaattc aaaggcgtgg cgaggtcgag tttgtccgtg
     4021 ccctccacgt atcgggccgg caggtcgtac ggcgaccgca ggtcaaaact cttcgcccgg
     4081 tcgtagagac cgtacagggt ggtttttgag ggggcttgag tgccattcat gggataccct
     4141 ttgaccgtgc cgtaggccag ccgcaaggtg aaggtggcgt ccgggtacac cgacttcccg
     4201 tacaccgtaa agcgcgccgt gccgattttc tccccggctg cagtttccac actttcaatg
     4261 ttgtcctcgt accatttgcg ctcttcgcgc aacagcggat caacccggcg ggccagaacg
     4321 atcaaaggat cggtggcagc cgctaccgcc gcctcgccac cgtccactaa ggacctgcgg
     4381 aaatcaggat cggccagctt cgttctggtg atgacttcct tggcaacctc tgccggagcg
     4441 cggccctgca gcagggcttt gataaaagga tcatccggcc cgagcgcgtc cagcgattcc
     4501 tggaagccat gcgtgagaag ggcctcttct aattgcggat agatcggtgc tggtgagaag
     4561 aggcggaagc gcatcgattc aagttgcgaa tcgcggtact cctggaatcg cttgccgtct
     4621 ggcttggtca cttcggttac gtaccggaca atggtcagtg ccagatccgg caggcgggag
     4681 ccgcgcagtc cgcggaacca cctctgggtg agcgtctttt gttgcttttt ctccgccttg
     4741 gcgatggcct cccaggcccg accgtattcc ttcttccagt ccgggttgct ggccaccagt
     4801 gcacgaaaat cttcttcctc ccttttcttt ttggccatca ggtttttatc cagaaggcca
     4861 ttgtactccc cggccaacgc cttaaggctg ttttcaatgc cgaagatttg tccagccgcc
     4921 tcgcgcgccg gctccggccc cctttcggcg tagcgtttca acacctctaa ccgtcgtttc
     4981 agagtttgga tgagcaatgg atagatatag tcgcgtgacc cttcgatctg cgccagcgtg
     5041 cgcaagcggc tggtggagcc ggggtggccc gaaacaaaca cgagctcgcc ctcggccgca
     5101 cctttggcgt tccatttcag atagtgccgg ctctcgaccg gcttatcgtt ttcatagacg
     5161 cggaagagcg ccatatccag atcgtagcgg gggtaggtga agttatccgg atcgccgccg
     5221 tagaaagcga tctgctgctc cggggcaaag accagccgga tgtcggtgta ctttttgtag
     5281 cggtacagcc agtactcgcc gccctggtag aaggagatca cgtcggagcg caggccggta
     5341 gagtctaaac tttctttctc gattttcgcc cgctcggctt tgcgcgcttc cagcgcctct
     5401 ttgtcgctca tccttttttt cacgaccccc tgcactcgtt cggtgacgtc tttcatggag
     5461 ataagcacgt tcagttcgag gtcgggacat ctcatctcct cggcctgcgt tttggcgtag
     5521 aatccctcct tcacataatc ctttccgggg gaggaaactt tctgcaactg gccgagggcc
     5581 acgtggtggt tggtgagcac cagtccgctg gggctgacga acgagccgga gccgccgtcg
     5641 ttgaaacgca cgctcgaaag ccgcacgtgg tcgaaccact cctgtgtcgg ggtgaagcgg
     5701 taacgctcct ggagttgttt cagcggcgga ttgtcaaagg tccacatccc ctcatcagcg
     5761 aaaaggagcg acccgccgcc ggtgaaggcc accacgccaa acgtcaaaac gaggaacatc
     5821 cccagatgtg atcgccgaac accatctttc ataagccacc tcccttagtg caaccgttct
     5881 ggaattgttg ccacattgtt ctgtctttct ttgccgcctt caccttaagg cacgaccccc
     5941 gtcccttact tttgaacttg cccggtcaac tctttcacct tcttttccag cgccttcacc
     6001 ctttccgcca actccggcag gcgcgatatc accgcttcga tccgtttttg cttgctcaat
     6061 tcccgcgccg ggtagccaaa gtaaaccgtg cccggcttca ggtctctatg gatgcccgac
     6121 ttggccgcca cgatcacgtt gtccccaatg gtgagatgtc ccaccaaccc cacctgtccg
     6181 gctaaggtca cgtttttgcc gattttggtg ctcccggaaa tccccacctg cgccacgatg
     6241 atgctgtttt cgcctatagc cacgttatga gcgatctgga ctaagttgtc gatcttcgtc
     6301 ccccgcccga tgatggtatt ccccatcgtc gcccggtcga tggtggtatt ggcaccgatt
     6361 tccacgtcgt cctcgacgac gactccgccc acctgcggaa ttttgtggta gacgccgcct
     6421 tccttggcaa agccgaaccc gtcactgcca acgaccgtac ccgagtggac aattacccgg
     6481 tcgccgatgg tgaccccctc ccgcgccgtg acattggggt aaatcaaaca gtcattcccg
     6541 attttggaat tgcgtcccac gtaggttccg gagaggagcg ctgaatgctc gccgactgac
     6601 acaccttccg ccaacacgac acaaggaccg aggtgaacgc cctccgccag ggtgacgttc
     6661 ttcccgacca cggcagtaga atgaattccc gggggataga gccgctcctg cggatggaac
     6721 aaccgcaggg ccacgagaaa ggcatagtag ggattcggat gctggatcaa cgccgttttg
     6781 atggaatcgg ttgcgacgtc gggtgatata atcaccgctg tggcctgcgt cccgacaagg
     6841 agcgaacggt atttctgatt ggcgacgaag gtcaactgcc ccggcccggc ggtttcaatc
     6901 cccgccacac cggtaatggc gacggccggg tcccccaaga gcgtcccgcc ggttttctgg
     6961 gcgatttctc ctaaggtcac gggcatgctc tacctgccgg ctaactcttg caggagtttg
     7021 tcggtcaaat cgaggccctt gcggccgtag gcaatgctgc cattcaccgc atcgaaaatc
     7081 aaaacgtaat tgttttccgt cgccagccgc tccaaggcct ggttgatccg gtctaaaatc
     7141 ggcttggtca gctctacgtt ccgccggtag tacttgcctg ccaccggatc gtagatttcg
     7201 gtgttgtagc gaaccagttc ctgttttttc agaccgatct cggattcttt ttccttccgc
     7261 ttctcgtcgg agaggaaaat ccgctgccgt tccagttccg tctccgaggt cgtgatcgcc
     7321 tgctggaggc tgtcggcgtg cttcttccag tcgaccgctt ccttgtccag cttggcctgc
     7381 gcgtccttga actctttcaa ttcgttgcgc agccggttgg agtcgatgaa gccgatcttt
     7441 ccctcctggc cgaaggccac accggcggta agaccgacca gggcggctgc gactgcaagc
     7501 atttgacgtg ctgacatact tttcctccgt ttcattgacc aaaatacaga aaaagtcttt
     7561 cagaagaaat ggtaatggta ggggtttgat taatcaaacc cctacctcag cgaaccgaaa
     7621 gggaaacacg agaattacgg aaatgaagca agggaactct tactgagagc actaaaacgt
     7681 cgtccccacc tggaagtggg gccgccagcg ccgcccttga acgaaccggc tctggtcaaa
     7741 accgtaggcg aagtcaaacc cgatcaggcc gatccccggc accgtcagcc ggatgccgaa
     7801 ccccgccgac ttgaacaggt gcgcaaacgg cgttacctcc cgcccggagg cccacgcatt
     7861 gccggcgtcg
//
LOCUS       DBNGZD010000246         5419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_661972, whole genome shotgun sequence.
ACCESSION   DBNGZD010000246 DBNGZD010000000
VERSION     DBNGZD010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_661972"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC16GM_06450"
     CDS             <1..270
                     /locus_tag="AC16GM_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR2923351.1"
                     /translation="IRENLKLFRRKGDFISTVNSSINEVLSRTIITSSTVLLVTAVLL
                     AFGGEVVRDFALALFMGVIVGTYSSIFVASAIVVEWENRSPKRFK"
     gene            complement(319..1860)
                     /locus_tag="AC16GM_06455"
     CDS             complement(319..1860)
                     /locus_tag="AC16GM_06455"
                     /inference="COORDINATES: protein motif:HMM:NF026288.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923352.1"
                     /translation="MIRVLIVLTYVFAFIGAISALLLGVSFTKGQLEKYPILTKLYGK
                     QVHLLVVTIICWAISFGANTWKDLLAVKPTVELSQGPLGELTQKATSAGLKKELEDAA
                     SEIARRAEDYFNAAERDFKTMRYLDAASNYQKSIDVIPTMSSFLNLGSSLFNISDFRK
                     AEDAFNSGLLIARKKENREFEGAFLGNIGNVYFYLGKPEEALDSYQASLKIYKQIGNS
                     LGEANALGNIGNVYFAQGKLGDALQWYEAAIKIDKRIGNRLGEGTTLGNIGNVYFVQG
                     DREKALKSYQAALEIDKQIGNPLGRAKHLHNIGNFYLTQGKGDEALKFYYTALKIHKQ
                     IGNLLGEANVLNNIGVIYKNEGKLDEALASYETALKIYEQTGNLLGQASALSNIGNIY
                     ALQGKQDDALRSHESALKVDKRTGDTMGQATDLNNIGNVFFNQGKWGEALKLYQEALE
                     IDKRTGNLPNQARDLRNIGNVYIKQGQKGAALEVLRRARAIYLKTGVAAKELQAVEEM
                     IESLN"
     gene            2227..3258
                     /locus_tag="AC16GM_06460"
     CDS             2227..3258
                     /locus_tag="AC16GM_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923353.1"
                     /translation="MEKKKAHAIALFSGGLDSSLAILLMRQQGIEVTALSFLTHFGCD
                     LSDRSSCGHNPYPVAEKFGFTVKLVHLGMEYVEAVKKPRYGHGKNMNICIDCRLIMLS
                     KAKEYMELTGADFVITGEVLGQRPMSQRWETLRMTDKQSGLQGRLLRPLSAKLLPPTV
                     PELEGLVDREKLLDIEGRSRHRQLELAKELGLEDYPSPASGCLLTDPGYSQRLRDLLA
                     HKTDADFTDINLLRVGRHFRLSPTCKVIVGRNDTENQIIKGLMRESEWLLEVRGTGSP
                     AVLFQLVGTNGNGSPDPEWLLTAARLTARYCDLKNQPEVEVTVTQNGSVESLTVSPSP
                     DDLVNSLRI"
     gene            3302..3376
                     /locus_tag="AC16GM_06465"
     tRNA            3302..3376
                     /locus_tag="AC16GM_06465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3337..3339,aa:Arg,seq:cct)
     gene            complement(3727..4386)
                     /gene="phoU"
                     /locus_tag="AC16GM_06470"
     CDS             complement(3727..4386)
                     /gene="phoU"
                     /locus_tag="AC16GM_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR2923354.1"
                     /translation="MERHFDEELNEIRKKLVAMAGLTEQLISRSIQVLVERDKSVAEE
                     VWPGEDSVNKKQIEIDEDCLRLLALHQPMAVDLRFLVATLRITGELERIADQAVNITQ
                     ASLVLIVRPEVKPLIDIPKMAALAAGMVKDSLDAFVKKDAGLAHAVLSRDDEVDAYKD
                     QIFRELLTYMLSDPKTIERGIELILISRHIERIADHATNIAEDAIYYVEGRDVRHHAG
                     E"
     gene            complement(4386..5231)
                     /gene="pstB"
                     /locus_tag="AC16GM_06475"
     CDS             complement(4386..5231)
                     /gene="pstB"
                     /locus_tag="AC16GM_06475"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR2923355.1"
                     /translation="MELPDVEWETVPEGPRLKLEPTRRNAADAAEQEKIVVERFSFWY
                     TKTKALNAVSTKVYQHQITAYIGPSGCGKSTFLRALNRMNDLIPYTRWEGKILLDGVD
                     ITRPQLDVTELRRRVGMVFQKSNPFPKSIFENIAFGLRIAGEISPRKLSVAVEEGLRQ
                     AALWDEVKDKLGRSALALSGGQQQRLCIARALAVKPEVLLLDEPCSQLDPISTAKIEE
                     LLLNLKQHYTIVLVTHNMQQAARISDYTGFFLLGELIEFDATPKLFRTPADKRTEDYI
                     TGRFG"
     gene            complement(5231..>5419)
                     /locus_tag="AC16GM_06480"
     CDS             complement(5231..>5419)
                     /locus_tag="AC16GM_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR2923356.1"
                     /translation="PNDQFMELGYHVYVMATQSPDVEKTKPILYATVFVLLALTFLLN
                     LLAIWLRARLRRRFAEER"
BASE COUNT         1210 a         1495 c         1334 g         1380 t
ORIGIN      
        1 atccgcgaga atttgaaact cttccgtcgt aagggtgact tcatctccac ggtcaactcc
       61 tccatcaacg aggtgctttc ccggaccatt atcacgtcat cgaccgtgct cttggtgacg
      121 gcggtgcttt tggccttcgg cggcgaggtg gtgcgcgact tcgctttagc cctctttatg
      181 ggcgtcatcg tcggcaccta ctcctccatc ttcgtcgcct ccgccatcgt ggtcgagtgg
      241 gagaacagat ctccgaagag atttaagtag ccccaaaacc ctaatttttg ctaattcttt
      301 ctttagaaat attcactgtt aattcaggct ttcaatcatt tcctcaacgg cttgcaactc
      361 cttagctgct acaccagtct ttaaataaat ggcgcgagcc cgcctgagca cttccaaggc
      421 cgcgcccttc tgtccctgct taatgtagac attgccgatg ttccgaaggt ctctggcctg
      481 attcggaagg ttaccagtcc gtttgtcaat ctcaagagcc tcttggtaca atttcagtgc
      541 ctccccccac ttcccttggt taaagaaaac gttgccgatg ttgttaaggt ccgtggcttg
      601 gcccatggta tcaccagtcc gtttgtcgac cttaagtgcc gactcgtgag accttagtgc
      661 atcgtcctgc ttcccttgga gcgcatagat attgccaatg ttgcttagtg cactggcttg
      721 gccaagcaga ttgccggttt gctcgtagat tttaagcgcc gtctcgtacg atgctagcgc
      781 ctcatccagc tttccctcgt ttttgtaaat gaccccaata ttattaagga catttgcctc
      841 gcccagaaga ttccctatct gcttatggat ttttagcgca gtatagtaga acttcagcgc
      901 ttcatccccc tttccctgag taaggtagaa gttcccaata ttgtggaggt gcttagctcg
      961 gcccagagga ttgcctattt gcttgtcgat ctctagtgcc gcctggtatg acttaagcgc
     1021 cttttcacga tctccttgca caaagtagac gttgccgatg ttccctaaag ttgtgccttc
     1081 gcccagacgg ttgccaatcc gcttgtcgat tttaattgcc gcctcgtacc actgcagcgc
     1141 gtcccccagc ttcccctggg caaagtaaac gttgcctata ttgccaagag cgttggcttc
     1201 gcctagacta ttgccaatct gcttgtaaat cttgagcgaa gcttggtacg agtccagtgc
     1261 ctcctcaggc ttgcctaggt aaaagtaaac attaccgata ttaccgagaa aagctccctc
     1321 aaattctcta ttttctttct tccgggcgat taggagtcct gaattaaaag catcttcagc
     1381 ctttcgaaaa tctgagatat taaaaaggga gcttccaaga ttgaggaaac tcgacatcgt
     1441 cggaatgaca tcaatcgatt tctgataatt acttgccgca tcaagatagc gcatcgtctt
     1501 aaaatcacgt tcggcagcat taaagtaatc ctccgccctc cgggcaatct ctgaagccgc
     1561 atcttctagc tctttcttta atccagcgga agttgctttc tgagtgagtt ctccaagcgg
     1621 tccttgactc aactccactg ttggttttac cgctagcaaa tctttccaag tgttagcccc
     1681 aaaggaaatc gcccaacaaa taatagtaac aactaataaa tgcacctgct ttccatatag
     1741 ctttgtaagt ataggatact tttccagttg gcctttcgtg aagctcacac ccagcaacag
     1801 cgcggagatg gctccaatga aggcgaaaac atacgttaga acaatcagaa cgcgaatcat
     1861 accatgtcct tccctgaact gtataaacca aaagcattaa aggatggttg tcaatgcgta
     1921 aagcaggaaa ctgaaaatgc ttgtttcaaa tctgctttaa cggcgttctt gaggccacac
     1981 cttgttaaaa atcctcgttt tctgcatgag agagggtgct tgacttgtgg aatttgaggt
     2041 cactccgggg gtaaaacgga caaaatgcca aataatagga ggcttatgtg tgtgccttgt
     2101 ggtttcctcc gtgtactccg tgcctccgtg gtgagatttg ccttgccttt caccaaatcc
     2161 caactcctaa atcccacctt ttccccgatt gaccaccctc accccatctg gtatcttaaa
     2221 attgctatgg aaaaaaagaa ggcccacgcc atcgcccttt tctccggcgg gctcgatagt
     2281 tcgcttgcca tcctccttat gcgccagcag gggatcgagg taaccgccct gtcctttttg
     2341 acccacttcg gctgcgacct ctccgaccgc tcctcctgcg ggcataatcc ctacccggtg
     2401 gcggagaaat tcggcttcac cgtcaagttg gtgcacctgg ggatggagta cgtggaggcg
     2461 gtcaaaaagc cgcgctacgg ccacggcaag aatatgaata tctgcatcga ctgccggctc
     2521 atcatgctca gcaaagcgaa agagtatatg gaattgaccg gcgccgattt cgtcatcacg
     2581 ggcgaagtgt tgggccaaag gccgatgtcc cagcgctggg agactctgcg gatgaccgac
     2641 aaacaaagcg gattacaggg gcgtttgctc cgaccccttt cggccaaact tttgccaccc
     2701 accgttccgg agttggaggg gctggtggac cgcgaaaagt tactggatat tgagggccgc
     2761 tcccgccacc gccaactgga actggctaag gaactggggc tggaggacta cccttctccc
     2821 gcatcggggt gtctcttgac cgatcccggc tactcccagc gcctccgcga tctgttggcg
     2881 cataagaccg acgccgattt caccgatatc aatctcctgc gcgtgggccg ccacttccgg
     2941 ttgtctccga cctgcaaggt aatcgtgggc cggaacgaca cggaaaacca gatcatcaaa
     3001 ggtctgatgc gagagagcga gtggcttctg gaggtgcggg gcaccggaag cccggcggta
     3061 ttatttcagt tagtcgggac aaatggcaac ggctcacccg atccggagtg gcttttgacc
     3121 gcagcacgtt tgaccgcccg ctactgcgat ctgaagaacc aaccggaagt ggaagtgacg
     3181 gtgacccaga acggttcagt ggagagcttg actgtctccc cctctcccga tgatttggtc
     3241 aactctttac gcatttgact ttcatcaccc atcactctgt cactaatcac tgttcttatc
     3301 cgcccccgta gctcaggatg gatagagcag cggtttccta aaccgttggc cggccgttcg
     3361 aatcggcccg ggggtacttt ttcaaaaccg tttgcgcgtc aagcggttca agccggtcga
     3421 ctgccgcgca tttgacgtcg gtgtttgacc ctgctacgac ttttgctacg acatttgcta
     3481 cagctcgagg tagttattgg aacattaaaa gcgttctact cgcatatcac gcttcactca
     3541 attatttaga aaatttaacg tagcacgctg gcatctaaca ggttgctacg aaaacatcga
     3601 ttggactaaa acagttctta gaacggagca gaggtctcaa aaaccgctgt ccaacgtatt
     3661 ttcttatacg tggcagttct gaatctgtaa atcgttcgaa ataatcggtg cacttttcgc
     3721 cttagattat tcgccggcgt ggtggcggac gtcgcgccct tccacgtagt agatggcgtc
     3781 ttcggcgatg ttcgtggcgt gatcggcaat ccgctcgatg tggcgggaga tcaagatcag
     3841 ctcaatgccc cgctcaatgg tcttcgggtc tgagagcatg taggtcaaca gttcccggaa
     3901 aatctgatct ttatacgcat ccacctcgtc gtcgcgggaa agcaccgcat gggcgagccc
     3961 agcgtccttc ttgacgaagg cgtccagact gtccttgacc ataccggcag ccagcgcggc
     4021 catcttgggg atgtcgatga gaggtttcac ttccggtcgg acaattaaaa ccagcgacgc
     4081 ctgggtgatg ttcactgcct gatcggcaat gcgctccagt tcacccgtaa tgcgtaacgt
     4141 cgcaactaaa aatcgcaaat cgactgccat cggttgatgc aaagccagca agcgcaagca
     4201 gtcttcatcg atctcaatct gctttttgtt gaccgagtct tcgcctggcc agacctcttc
     4261 ggccacggat ttatctcgct ccaccaagac ctggatcgag cggctaatca actgttccgt
     4321 caggccggcc atggcgacta actttttccg gatttcattc aactcttcat cgaagtggcg
     4381 ttccattatc cgaacctccc ggtgatatag tcctcggttc tcttgtccgc tggcgtccgg
     4441 aagagcttgg gtgtggcgtc gaactcgatc agttcaccca agaggaaaaa gccggtgtag
     4501 tcggaaatgc gggccgcctg ctgcatgtta tgggtgacca ggacaatcgt atagtgctgt
     4561 ttgaggttga gcaatagctc ctcaattttg gcggttgaaa ttgggtccaa ttgggagcag
     4621 ggttcgtcca ggaggaggac ctccggcttg accgccagcg cccgggcgat gcagagccgc
     4681 tgctgctggc ctccggaaag agcgagcgcc gaccggccca acttgtcctt tacctcatcc
     4741 cacagagcag cctgccgcaa accctcttct accgcaacgg aaagtttccg ggggctgatt
     4801 tcccctgcaa tccgcaggcc gaaggcaatg ttctcaaaaa ttgatttggg gaacgggttc
     4861 gatttctgaa aaaccatgcc gactctgcgc cgtaattcag ttacgtcgag ctggggacgt
     4921 gtgatgtcaa ccccatccaa gagtatcttg ccttcccacc gggtgtacgg tatcaaatca
     4981 ttcatccggt ttaaggcccg cagaaacgtc gacttgccgc agccggaagg gccgatgtac
     5041 gcagttattt ggtgctggta aaccttcgtg ctgaccgcat tgagggcctt tgttttcgtg
     5101 taccagaagg aaaagcgttc gacgacgatt ttctcctgct cagccgcatc ggccgcattc
     5161 cgtctggtgg gctcaagctt taaccgcgga ccctcaggca ccgtttccca ttccacgtca
     5221 gggagttcca ttaccgttct tccgcaaacc gccggcgcag acgtgctcgc agccagatgg
     5281 ccagaaggtt caaaaggaac gtcaaagcca gcaaaacgaa aactgtcgcg tacagaattg
     5341 gtttggtttt ctcgacgtcc ggggattgag tcgccatcac gtagacatgg tatcccaatt
     5401 ccatgaactg atcgtttgg
//
LOCUS       DBNGZD010000247        14128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1705359, whole genome shotgun sequence.
ACCESSION   DBNGZD010000247 DBNGZD010000000
VERSION     DBNGZD010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 14128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14128
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1705359"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..538
                     /locus_tag="AC16GM_06485"
     CDS             143..538
                     /locus_tag="AC16GM_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923357.1"
                     /translation="MRQSLHHFWRFFGFWFLGGPALVTLFLVSCSGEGKVTLLNDTFF
                     SPLQYQIVAVASGEPAAQGTLAADQQVTRTIKEGTYRILFRSEGQTDDFCSEDTVTVS
                     GGSGCSTGPELRTTPVLGDCGGGHGGHGG"
     gene            535..2040
                     /locus_tag="AC16GM_06490"
     CDS             535..2040
                     /locus_tag="AC16GM_06490"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HIR2923358.1"
                     /translation="MRNSFSKEHSTGHPPRDAGAVHSAQKMRIGSALILLAVVTFSAC
                     SDKKIVSPPPEPPAVFSRDVLPIFNRSCNRSGCHISGDTLVNPSRFFLDSFDSLRFSA
                     KTLLAEKPDHPVIIPGLGVESHLVLHLLGIDTPLMPLGGPPLPRNEIKKVIQWINEGA
                     RGDNGEPFYDPAGKIYVANSGQGTVTVIDPDLNLILKTIPVARNPNNAAAEFAHHFAW
                     NFTDNFLYLTMAGQLGELVKIDPGSDQIVARLAVGVAPTDVRISPDGRFAYVANHDLN
                     PFLPRSTVTKINLTTFTVAGTITVGKAPHGLRMSQDGRFMLACNYLTDDVTFIYLNTN
                     GDADSTFTLKLGSAPKIFRPYGIGFSPDDSLAFVSCQQSNEVRVVDLKNRLIVDSIPV
                     GFEPFMVERTPDNRFLYVPCRQSDAVYIIRLSDRTVVKSVGVGFRAHGVDFSPDGLRA
                     YITCEGDHINPSFIYVINAVTQRIIGPIEVGVFPNGVLVSPLASTSQFARR"
     gene            2085..2969
                     /gene="prmC"
                     /locus_tag="AC16GM_06495"
     CDS             2085..2969
                     /gene="prmC"
                     /locus_tag="AC16GM_06495"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR2923359.1"
                     /translation="MARTAKEALKEVRAQLTPRSGDGARVEAELLLSSVLGIPRVELY
                     IDAQRELTPAEEKQLEAMVAERLTGKPVQYILGLAEFYGLPFRVDPRVLIPRPETEVL
                     VEVMLNFFKEHGLARSPLVIVDLGTGAGNMAITLAKLLPQARLYATDISSEALEVARK
                     NAEENQVKEQLIFLAGDLFEPLQDLNLGGKVDAFVSNPPYVREIEWPILPAEVREFEP
                     KAALYSPGSGTFFHKRIIQKAPEYLKSGGGVFLEVAAGQTEEVKHFFQKTPGYRDIRV
                     LKDLGGIDRVVWARRLTD"
     gene            2979..3761
                     /locus_tag="AC16GM_06500"
     CDS             2979..3761
                     /locus_tag="AC16GM_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923360.1"
                     /translation="MLWIFLIAILVFGFPPTAAGQDEFNRHPESKSPSIEPAANRLLF
                     MSTTEVLPHGTWQYSNYYVGSSHLALGLAGPVQIGGSLTLFPLLTPIVAYSGGVKAKL
                     WSPASSHPGLAVSVTYLRILIAAAYSPQFISCPPNQPCEVRDQSSPESARFWTLSAVA
                     SYRWRRFGYHVEFQRLLAIEKDEDNVWTMTLGMDVHLLNWVRVLAEASRGAADYRPAG
                     SWVIGGAVRLLSRRGAFDLGGLGTHEDQSTYLWPFLSFSLLF"
     gene            3952..5196
                     /locus_tag="AC16GM_06505"
     CDS             3952..5196
                     /locus_tag="AC16GM_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR2923361.1"
                     /translation="MKAIVKEKPKEGQPWPRGLHLVEKAVPKVLHPDDVKVEVIAAGI
                     CGTDVGIYNSKDSIRVEMAKLLQDSVTIGHEFCGRVVDAGEGARLYFARQLADRHWTG
                     DDDPALLRLVQSRSPDAIAADSHLIDLLGQHFLLSAEMHQTCGHCYQCRRGERHVCQN
                     TVIKGIHDDGAFARYMLTSGRNIILFEKDELPVEIIAFLDAFGNAVHTAQATRLEGRT
                     VAVLGCGVQGLMSTAIARYCGAAKIFATDAANPEKGLTPEKLAKKRFTLAAKLGADCC
                     FDVGTPQGHGEFVEAVKAETHGTGVDVVFEMSGSYNAYRDAFDVVRMGGTVALLGIPE
                     GEWPLDFSKKVIFPGVTIQGIIGRRVFETWEVMRAMLKAGVAQQLLGCGFVSHQFPLE
                     KFEEGFAVICSGDGLKVLLRPT"
     gene            5359..6546
                     /gene="kbl"
                     /locus_tag="AC16GM_06510"
     CDS             5359..6546
                     /gene="kbl"
                     /locus_tag="AC16GM_06510"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIR2923362.1"
                     /translation="MIGTVRDFLTKELTGIREAGLYKEERVILSPQGAEIRVREGEVI
                     NFCANNYLGLGNRPELIAAAKQALDSHGFGLASVRFICGTQDIHKELEKKISEFLRTE
                     ATILYTSCFDANGGLFETLLSDDCVVISDELNHASIIDGIRLCKAERRRFKHADMGDL
                     EAQLQGNEKFRIRLVATDGVFSMDGDIAKLDQICALAEKSQALVMVDDSHATGVLGQT
                     GRGSIEHCGVVGKVDIITSTLGKALGGASGGFTSGRKEIIEMLRQRSRPYLFSNTLAP
                     PLVAASIQVLELLSQTTDLRERLEDNTRYFRNEMTKRNFDIKPGVHPIVPIMLGEAKL
                     AQDMARDLLKEGIYVIGFFYPVVPKGQARIRVQVSAAHTRAHLDKAVEAFTKVGRRYG
                     VIA"
     gene            6685..8028
                     /locus_tag="AC16GM_06515"
     CDS             6685..8028
                     /locus_tag="AC16GM_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR2923363.1"
                     /translation="MPSLSLDWEKIRPDAVSTLQSLVQFNTVNPPGAEEPVIDYLQDL
                     LQKEGIATEILAAEPTRDNLFAHLPGSSKEPPLLLMSHVDVVGVEPEKWSVHPFSGAI
                     DNGYLYGRGAIDDKGMAAAEAMVLLLLKRLNVKLKRDVVYLAAADEESGGKLGIAWLV
                     DKHRDKLQVEYAINEGGRIRTDDSGQVRYVCIQNTEKVPYNLTLKVKGTPGHASVPLP
                     DNPIFALSQALAKIVNYKAPLKLIPTTEKFFAGLAKKEKYPASYYLENANHPLVGQLC
                     REALAKMHPVFHSMLCNSISPTVFQAGIRANVIPSEAQVNLNCRLLPGEKITHFVEEV
                     KRVISDPRIEITYEVGKQPDSPESPVDSPVYRALETNAKKLWPEAEVVPLLSTGATDS
                     AELRAIGVKSYGILPFPLSENDEARMHGHDERIPLDGYLEGIKYLFNTTVELAAE"
     gene            8136..9284
                     /locus_tag="AC16GM_06520"
     CDS             8136..9284
                     /locus_tag="AC16GM_06520"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR2923364.1"
                     /translation="MLTKAEPVLKTEKEKRLAAHEIEHRTGHPYHMYDSILLQPTMVR
                     EALAANEKNVGKVAGALAKKKRLYLVGIGTSSHAAIAGEYFFRVLCGGKLDAQFIQAF
                     EFVHYPPRLDRETAVLIVSHRGWKTFSEQATKMAREQGALSISVTGRNPQREVILGEH
                     VLFTSEQEKSHAHTMSSTAALAVLLQLAQATGKRVGAKQQETFELSNLPDLLEASLAL
                     EPQTRNLAGRLCDCERFFFVAFGPSVATALEAALKMQETTFTASAGYEVEQFLHGPLA
                     SVSPESAVFFIAPPGLGQKRCWQALNAVKAVGAKTVLLTQKNEISLAPAADETILLPE
                     MNELVTPIVYVVPVQLYSYFTAIKLGHNADVNHRDDLRYQKASEAFAL"
     gene            9325..10215
                     /locus_tag="AC16GM_06525"
     CDS             9325..10215
                     /locus_tag="AC16GM_06525"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR2923365.1"
                     /translation="MKTAKETAIESEPGVLIQVEEQPVPATETSVGIKHAIAVTSGKG
                     GVGTSAVAVNLAVALAQQGLEVGVVDFDHDGPSVAKMLGIEPQKFAAGDNGVLPVTGP
                     LGIKVLTFDLCQSAAAPQNSATSENGVVSNGTPAPNLAVALANTDWGALDYLFIDVPS
                     ETDDLLQVARTIPALDGILVVTIPAEISHLIVKKSIALTRGVTAAPVIGLVENMQGYF
                     CTHCGQTGELFPQGNGRVTARELGIPYLGGIPFDPRLADAGDRGAPYLVTYRDTATGQ
                     AILKIAKSVTQFFADKNHKS"
     gene            10273..10737
                     /locus_tag="AC16GM_06530"
     CDS             10273..10737
                     /locus_tag="AC16GM_06530"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR2923366.1"
                     /translation="MRLLNLILAWLHLISAFTLVGGSIFVGFVLAPAAKAQSGNWIVT
                     LAATEKRYRKLARRMVEVVILTGVANLLLHRLAFHMPWTGHYLGTIALKLALVLTMVF
                     LYARRVKILTALADKGTLGEVENNELKRADQLIGWQMVLGAVVILLGLSLRG"
     gene            complement(10785..11141)
                     /locus_tag="AC16GM_06535"
     CDS             complement(10785..11141)
                     /locus_tag="AC16GM_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2923367.1"
                     /translation="MQKIESFQQLNVWQASHRLALEIYLLTKQFPREEKFGLVSQMRR
                     AAVSVPANIAEGFKKRGTKDKINFYNISQGSLEELRYYLILSRDLGYLKDHAATEQKT
                     EIISRMLTALIRSLSE"
     gene            complement(11180..12118)
                     /locus_tag="AC16GM_06540"
     CDS             complement(11180..12118)
                     /locus_tag="AC16GM_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR2923368.1"
                     /translation="MPTPSAASQDYRRFLVPEVISKLKGMELKARLVVEGFIAGLHRS
                     PYHGFSVEFAEHRQYMPGDPVRNIDWKVFAKSDRYYIKEFEEETNLKAYLLLDTSGSM
                     GYSSQDQSPEVKSRITKLEYASYLAAALSYLMLLQRDSVGLVTYADKIKRYIPPKAKR
                     DHLHVLLRELDGALPEKTTNTATALHEMAERIKRRGLIILLSDLFDDPDRVLSGLKHF
                     RHRKHEVILFHILDPLERTFAFPGEAVFKDMETGEEITTLPWQIKRDYQARLKTLTDR
                     YLYECRKSQIDYQQLDTATPFDKALLAYLIKRGRLY"
     gene            complement(12135..12542)
                     /locus_tag="AC16GM_06545"
     CDS             complement(12135..12542)
                     /locus_tag="AC16GM_06545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2923369.1"
                     /translation="MSGIISLRFSESKDIDVDKLIDLYQFGWWSNQRKKPDVKKMLDH
                     TPMVISVWDGDLLVGFTRLLTDCTYRATIWDVIVRPEYQSQGIGRKIMEYILTHPRLK
                     TVTFFWLSTRDKHAFYEQFGFVRDQTDSLILKR"
     gene            complement(12558..13568)
                     /locus_tag="AC16GM_06550"
     CDS             complement(12558..13568)
                     /locus_tag="AC16GM_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2923370.1"
                     /translation="MPEAVRTTGDLEAVEKLKAAKEIIKKEIGKVIVGQERVIDELMI
                     ALLSGGHCLIIGVPGLAKTLLISTLAKVLDLKFSRIQFTPDLMPSDITGTEILEEDRT
                     TGKRLFRFVKGPVFANIVLADEINRTPPKTQAALLQAMQEYEVTASGETFKLGLPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMFNINIDYPSSAEEAEIVRTTTSAYAASLDKVATG
                     VEIIRLQKLARRVPVADHVINYAVRLVRATRPVENGTYEYIKNWISWGAGPRASQYLI
                     LGGKVKAILDGRLTPSTEDVRFVAPPVLRHRIVTSFNAEAEGVGTEQIVQRLLSDVQD
                     KP"
     gene            complement(13775..14095)
                     /locus_tag="AC16GM_06555"
     CDS             complement(13775..14095)
                     /locus_tag="AC16GM_06555"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR2923371.1"
                     /translation="MGLYPTGKMVISPEFDFALPFSEGLACVEVGAKSGYINKTGKFA
                     ISPQFDFAWNFSEGVAPAEIGGKSGYIDKTGKFVIKSSFNEARDFSEGLACVKVGGSR
                     YSSD"
BASE COUNT         3029 a         3884 c         4053 g         3162 t
ORIGIN      
        1 tttcttcggt gattggttgg acagcgacca aattcgcggt tgaacgtgcg aaagtctacc
       61 gcgggctttt gtgtggattc tcggctgcgg ccatcgtggt cggcggcgtg tggcttgttt
      121 tgtagaatgg agaaggcgta aattgaggca atccttgcac catttctggc gttttttcgg
      181 cttctggttt ctggggggac cggcgttggt tactctcttt ttggtcagct gctccggcga
      241 aggaaaagtg acgcttttga atgacacttt cttttctccg ctccaatatc aaattgtggc
      301 tgtagcctct ggtgaaccgg cggcgcaagg gacgctggcg gcagaccagc aggtaacccg
      361 gacgatcaaa gagggaacct accggattct tttccgctct gaaggacaga ctgatgattt
      421 ctgctcggaa gacacggtga cggtttccgg cggctccggc tgcagcaccg ggccggaact
      481 gagaacgacc cccgtcttgg gtgactgcgg gggcgggcac ggtggacacg gcggatgaga
      541 aattcatttt ccaaggagca cagcacaggt cacccgccgc gtgacgcagg agcagtccac
      601 tcggcgcaaa agatgcggat cggctctgct ctgatcctgt tggcggtggt gacattttcc
      661 gcctgtagtg acaaaaaaat tgtttcccca ccgccggagc cgccggccgt tttctctcgt
      721 gacgttttgc caattttcaa ccgttcctgc aatcgatccg gctgccacat ctccggggat
      781 acgctggtca atccatcgcg gtttttcctc gactcctttg acagcctgcg gttctccgcc
      841 aaaacgctct tggcggagaa accggatcat ccggttatca tccccggcct gggcgtggaa
      901 agccacctcg ttttgcacct gctgggtatc gatacgccct tgatgccctt gggcgggccg
      961 cctttgccgc gcaatgaaat caagaaggtc atccagtgga ttaacgaagg cgcgcggggt
     1021 gataacggag agcccttcta cgatccggca ggaaaaatct acgtggccaa cagcggccag
     1081 ggaacggtga cggtcataga cccggactta aaccttatcc tgaaaactat ccccgtcgcc
     1141 agaaatccga acaatgctgc ggctgagttc gcccaccact ttgcctggaa ctttaccgac
     1201 aacttcctct acctcaccat ggcagggcag ttgggcgagt tggtgaaaat tgatccgggg
     1261 agcgaccaga tagtggcgcg ccttgccgtg ggcgtggccc cgaccgatgt ccgtatctca
     1321 ccggacgggc gctttgccta cgttgccaac catgacctga atccgtttct cccccgctcg
     1381 acggtgacca agattaatct gacaactttc acggtcgccg gcaccatcac tgtaggcaag
     1441 gcgccgcacg gcttgcggat gtctcaggat ggccggttca tgctggcctg caattatttg
     1501 accgatgatg tcacgttcat ctacctgaac accaacggcg acgccgattc cactttcact
     1561 ctcaagctgg gctcggcccc gaagatcttc cggccctacg gcattggatt ttcgccggac
     1621 gacagcctgg cgttcgtctc ttgccagcag tcgaacgagg tgcgggtggt tgatcttaaa
     1681 aaccgcctca tcgttgactc cattccggtg gggttcgagc cattcatggt tgaaagaacg
     1741 cccgataacc gattcctgta cgtcccctgc cgacagagtg acgcggtgta catcatccgc
     1801 ctctccgacc gcacggtggt caaaagcgtg ggggtcggct tccgggctca cggggtcgat
     1861 ttcagtcccg acggcctccg ggcctacatc acctgcgagg gggatcacat caacccctct
     1921 ttcatctacg tcatcaacgc cgtcacccaa aggatcatcg ggccgatcga agtaggggtg
     1981 ttccccaacg gggttttggt cagcccgctg gcgtctacga gtcaattcgc gcgacggtag
     2041 ttttccgctt ttctccgaat tctccgcata tattgtctct ccggatggcc aggaccgcca
     2101 aagaggccct caaggaggtg cgggcgcaat taactccccg ttccggcgat ggcgcccggg
     2161 tagaggcaga acttttgctt tcgagcgtcc tcggaattcc ccgcgtggaa ctatacatcg
     2221 atgcacagcg ggagttaact ccggcagaag agaaacagct ggaagcgatg gtggctgaac
     2281 ggctgacggg caaaccggtg cagtacatcc tggggctggc cgaattctac ggtctgccct
     2341 ttcgggttga tccccgcgtt ctcattcccc gacccgaaac agaagtgttg gtcgaggtga
     2401 tgctgaattt tttcaaagaa cacggcttgg cacgatcacc tttggtcatc gtcgatttgg
     2461 gcacaggcgc ggggaacatg gccattacac tggctaagct tttgccccaa gcccgcctct
     2521 atgccaccga catctcgtcc gaagcactgg aggttgcgcg gaagaacgca gaggaaaatc
     2581 aagtaaaaga gcagctcatt tttctcgcag gtgacctctt tgaaccgctg caagatttga
     2641 atttgggggg aaaagttgat gcctttgtct ccaatccccc ctacgtacgg gaaatcgagt
     2701 ggccgatctt gccggcggag gtgcgggagt tcgaaccgaa agcggctctc tacagtccgg
     2761 gttccgggac ctttttccac aaacgtatta tccaaaaagc gccggagtac ctcaaatcag
     2821 gcgggggggt attcctggaa gtggccgcgg gtcagaccga ggaagtgaag catttcttcc
     2881 agaagacacc cgggtaccga gacattcgag tcctgaaaga cttaggcggg attgaccggg
     2941 tggtctgggc acgtagattg actgactgag gtggtatcgt gttgtggatt tttcttatcg
     3001 caattctggt ctttggtttt cctccgaccg ctgccggtca agacgagttt aatcgacacc
     3061 cggaatcgaa gtcaccgtcg attgagccag ctgctaatcg gctgctgttt atgtctacta
     3121 cggaggtact tcctcacgga acgtggcagt attcgaacta ttacgtgggt tcttcccatc
     3181 tggctttagg gctggctggc ccggttcaaa ttggcggctc gttgaccctg tttcctcttt
     3241 taactccgat cgttgcctat tcggggggcg taaaggcaaa gctctggtct ccggcatcgt
     3301 cacatcccgg cctggccgta tcggtcacct acctccgaat tctgattgct gccgcatatt
     3361 cgccgcagtt catttcctgt ccgcccaacc aaccctgtga agtgcgagat cagagcagtc
     3421 ctgaatcagc acgtttctgg acattatccg ccgttgcgag ttaccgctgg cgccgattcg
     3481 gataccacgt tgaattccag cgtcttttag ccattgaaaa agacgaagat aatgtttgga
     3541 ctatgacctt gggaatggat gttcatctcc ttaattgggt ccgcgtactg gccgaggcgt
     3601 cgcggggggc ggccgactac cggccggccg gttcctgggt catcggcggc gccgtgcggc
     3661 ttctgagccg acgcggcgcg ttcgatctgg gtgggctggg aacgcacgaa gatcaaagca
     3721 cttacctctg gcccttcctc agtttctctc tgttgtttta gagcgatcag gttcgggaag
     3781 caatagttat tctgtcttgt tgttctgaac tttattgact tccattcaaa tctcttttaa
     3841 ttaccgccgt acgtttgcat ggaggttgac atgccacccg tagccaccaa agagcgagcg
     3901 ccggttcccc aaaccaggcc cagagttcat gcggatagac ccgcaggttt catgaaggcc
     3961 atcgtcaaag aaaaaccaaa ggaggggcag ccctggccgc gaggtctgca tttagttgaa
     4021 aaagcggtcc caaaagttct gcatcctgac gacgtcaaag tcgaagtgat cgccgccggc
     4081 atctgcggca ccgacgtcgg catctacaac agcaaagact ccatccgggt cgaaatggcg
     4141 aaattgctcc aagattcggt caccatcggc cacgaatttt gcgggcgggt ggtggacgca
     4201 ggcgaagggg cacgtctcta ctttgcccgc cagttggccg accgccactg gaccggcgac
     4261 gatgatccgg cccttttacg tttagtccag agtcgatctc cggatgccat cgccgccgat
     4321 tcgcatttaa tagatctgct cggtcagcac ttcctccttt ccgccgagat gcatcagact
     4381 tgcggccatt gctaccagtg ccggcgcggg gaaaggcacg tctgccagaa cacagtgatt
     4441 aaggggatcc atgacgacgg ggcgttcgcc cggtatatgc tcacctcggg gcgcaacatt
     4501 attctttttg agaaagacga actgccggtg gaaatcattg ctttcctgga tgctttcggc
     4561 aacgcagtcc acacggcgca agcgacccgc ctggaaggcc ggacggtagc agttctgggg
     4621 tgtggcgtgc aggggttgat gtccaccgcg attgcgcgtt actgcggagc ggcgaaaatt
     4681 tttgccaccg atgcggccaa tcccgaaaag gggttaacac cggagaaact ggccaagaaa
     4741 cgcttcaccc ttgcggccaa actgggcgcc gactgctgtt ttgacgtggg aaccccccag
     4801 gggcacggtg aattcgtcga ggcggtcaag gccgagaccc acggcaccgg cgtggatgtg
     4861 gtctttgaga tgtccggttc ctacaatgcc taccgggatg ccttcgacgt ggtgcggatg
     4921 ggcggcacgg tggcgctttt gggtattccc gaaggggagt ggccgctcga tttctccaag
     4981 aaggtgatct tccccggggt gacgattcag ggaatcatcg ggcggcgggt gttcgagacc
     5041 tgggaagtga tgcgggcgat gctcaaagcc ggggtggccc agcagttgct cggttgcggc
     5101 ttcgtctccc accaattccc tttagaaaaa ttcgaagaag gctttgctgt catctgctcc
     5161 ggggatgggt tgaaggtgct tttgcgacca acgtgattgg agattttgta aaatttgcag
     5221 cgcgggatgg ttctgaatcc cgcgctattt ttcgcgcagg ttacaccctt cggcttcgct
     5281 cagggcaagc caacctgcgc tacggttcag ataggaccgc tgagtcaacc cgttaacgag
     5341 aaaaagcagg gataaacgat gatcggaaca gtccgagatt ttctcactaa ggaactcacc
     5401 ggcattcgtg aagcggggct ctacaaggaa gagcgggtga tcctcagccc gcagggggcc
     5461 gagattcgcg tacgggaggg cgaggtcatc aatttctgcg ccaacaatta cctcggtctg
     5521 ggcaaccgcc cggagttaat cgcggcggcc aagcaggcgc tcgattctca cgggttcggc
     5581 ctggcctcag tgcgcttcat ctgcggcacg caggacatcc acaaagaatt ggagaaaaaa
     5641 atttctgagt ttctccggac cgaagccacg attctttata cctcctgttt tgacgccaac
     5701 ggcggtttgt tcgaaacgct tttgtccgac gattgtgtcg ttatctccga cgaactgaac
     5761 cacgcctcga tcatcgacgg catccggctc tgcaaggcgg agcggcggcg ctttaagcac
     5821 gccgatatgg gggacctcga agcgcaactg cagggtaacg agaagttccg catccggctc
     5881 gtcgccaccg acggcgtctt ttcgatggat ggcgacatag cgaagttaga ccagatttgc
     5941 gcgctggctg agaagtctca ggcgttagtg atggtggacg attcccacgc caccggggtc
     6001 ctcggccaga ccggacgcgg ctccatcgag cactgcgggg tggtgggaaa agtggacatc
     6061 atcacgagca ccttagggaa agcgctgggc ggggcttccg gcgggttcac cagcggacgg
     6121 aaggaaatca tcgagatgct gcgccagcgc tcgcggcctt atcttttctc caacaccctg
     6181 gcgccgcctt tggtggcggc gtcaattcaa gtcttagaac ttttaagcca gacgacggac
     6241 ctgcgtgaac gtcttgagga caatacccgt tactttcgca acgagatgac taaacgtaac
     6301 tttgacatca agcccggcgt gcatccgatc gtcccgatta tgctcggcga ggcgaaatta
     6361 gcccaggata tggcccgcga cctgctcaaa gagggaattt acgtcatcgg cttcttctat
     6421 ccggtggttc ccaaaggcca agcgcggatt cgggtgcagg tttccgccgc gcatacccgg
     6481 gcgcatctgg ataaggcggt cgaggcgttt accaaagtgg ggcggcggta cggggtgatt
     6541 gcgtaattgg gttaacagcg gcgctggtag tgccgcctac gcgaggtaga gaaaggtcac
     6601 aacccgcgaa ggcgggtcgg cacgactcgt accgaggctc ttagccccgc ataaaaaaga
     6661 gattattata aagaggtgag cagtatgcct tccctttcgc tcgattggga aaagatcagg
     6721 cccgatgcgg tttcaaccct gcaaagttta gtccagttca acaccgtcaa tccgccgggg
     6781 gcggaggagc cggtgattga ctacctccag gatttgctac aaaaagaagg gatcgccact
     6841 gagattctgg cggcggagcc gacgcgggat aacctttttg cgcacctgcc cggctcaagc
     6901 aaagagccgc cgttgctttt gatgtcccac gttgatgtgg tcggcgtgga gccggagaag
     6961 tggtcggtgc atcccttctc cggggccata gacaacggct acctctacgg ccggggtgcc
     7021 atcgacgaca aaggaatggc cgccgccgag gcgatggtgc tcttgcttct gaaacggctg
     7081 aacgtcaaac tcaagcgcga cgtggtttac ctcgcagcgg cggatgaaga aagcggcggg
     7141 aagctgggca tcgcctggct ggtggacaaa caccgggaca aactgcaagt cgagtacgcc
     7201 attaatgaag gtggccgcat ccgcaccgac gattccggcc aggtgcggta cgtctgcatc
     7261 cagaatacgg agaaggtgcc gtacaatttg acgctcaagg taaaaggtac ccccggccac
     7321 gcctcagttc cgctccccga taaccccatc tttgccctct cgcaggcgtt agcgaagatt
     7381 gtcaattaca aagcgccttt gaaactgatc cccacgacgg agaaattctt cgccggtctg
     7441 gccaaaaagg agaagtaccc ggccagttac tacttggaga acgccaacca cccgctggtc
     7501 ggccaactct gccgcgaggc gctggcaaag atgcatccgg ttttccattc gatgctctgc
     7561 aattcgattt caccgaccgt cttccaggcc ggcatccggg ccaacgtgat tccctccgaa
     7621 gcgcaggtga acttgaactg ccgcctctta ccgggcgaaa aaattactca tttcgtagaa
     7681 gaagtgaagc gggtaatcag cgacccgcgg atcgaaatca cctacgaggt gggcaagcag
     7741 cccgattcgc cggagtcgcc tgtggacagc ccggtctacc gggcgctgga gaccaacgcc
     7801 aaaaagctct ggcccgaggc ggaggtggtg ccgctcctct ccaccggcgc caccgattcg
     7861 gcggagctgc gcgctatcgg cgtcaagtcc tacggcatat tgccttttcc gctctccgaa
     7921 aacgacgaag cccgcatgca cggccacgat gagcgaattc cgctggacgg gtaccttgag
     7981 ggaattaaat acctgttcaa cacgacggtg gaactggcgg cggagtaagt ggaactccac
     8041 gagccgccgc tggaagtgag ccgatagatt tttctcgtca ttttgacgac cggcgggtgt
     8101 atcctatccg aaatttcgtc cgaaatagaa aatagatgct gacgaaggct gaaccggtac
     8161 tcaagacaga aaaagaaaaa cggctggcgg cgcacgaaat cgagcaccgc accggccacc
     8221 cgtaccacat gtacgattca attctgttgc aacccacaat ggtgcgggaa gcgctggccg
     8281 ccaacgagaa aaacgtcggc aaggttgccg gagcactggc aaaaaagaaa cgcctctacc
     8341 tggtcggcat cggcacctca tcccacgccg ccattgcggg ggagtatttc ttccgcgtgc
     8401 tctgtggggg aaaattagac gcgcagttca ttcaagcctt tgagttcgtc cattacccgc
     8461 cgcgtctcga ccgggagacc gccgtcctca tcgtcagcca tcggggctgg aagacttttt
     8521 cggagcaggc gacgaaaatg gctcgcgagc aaggcgcact ttcgatttcg gtgacgggca
     8581 gaaatcctca acgggaagtt attttgggtg agcacgtcct cttcaccagc gagcaagaaa
     8641 aatcacatgc tcacacgatg agttctaccg ccgcgctggc agttttactc caattagctc
     8701 aggcgaccgg gaaacgggtc ggtgcgaagc agcaggaaac atttgaactg tcgaatctgc
     8761 cggatttact ggaagcatca ctggctctgg aaccacagac acgcaatctg gcgggtcgtc
     8821 tctgcgactg cgaacggttt ttcttcgtag ctttcgggcc aagcgttgcc acagcgctgg
     8881 aagcggctct gaaaatgcag gagactacgt tcaccgccag cgccggctac gaagtcgaac
     8941 aatttctcca cgggcctttg gcgtcggtca gtcccgagtc ggcagttttc ttcattgcgc
     9001 cgcccggatt gggacaaaag cggtgctggc aggcactgaa cgccgtgaaa gcggtcggtg
     9061 cgaagacggt tcttctgacg cagaaaaatg aaatttcgct tgcacctgca gcggacgaaa
     9121 ctattttact ccccgaaatg aacgaactcg tcacaccaat tgtgtacgtc gttccggtgc
     9181 aactttattc ttactttaca gcaattaagt tgggccataa tgccgatgtt aatcatcggg
     9241 acgacctgcg ctaccaaaag gcgagcgagg cgttcgcttt gtgaaacgta ccggccggtg
     9301 aaaaaagaaa gcaggaacga agaaatgaaa acagcgaaag aaaccgcgat tgaatcagaa
     9361 cccggcgtgt tgatccaggt ggaagagcaa ccggtgccgg cgaccgaaac gtcggttggg
     9421 atcaagcacg ccatcgcggt caccagcggc aaaggcggcg tcggcaccag cgcggttgcc
     9481 gtaaatctgg cggtggctct ggcccagcag ggcttggaag tgggcgtggt cgatttcgac
     9541 cacgacggtc cttcagtggc gaagatgctg ggtatcgagc cgcagaagtt cgcggccggt
     9601 gacaatggcg ttctgcccgt caccggtcct ttgggaatca aagtactgac ttttgatttg
     9661 tgtcaatctg cagcagctcc gcagaattcc gccacgtccg aaaatggcgt agtctccaac
     9721 ggcacgcctg cgccaaacct ggcagtagct ctggccaata ccgactgggg cgcgcttgac
     9781 tatttgttca tcgatgttcc ttcggaaacc gatgatctgc tgcaggtggc gcgcaccatt
     9841 cccgctctcg acggcattct cgtggtgacc attcccgccg aaatttcgca cctgatcgtt
     9901 aagaaatcga ttgccctcac caggggagtg accgctgccc cggtgatcgg cttggtggag
     9961 aacatgcagg ggtacttctg cacccactgc gggcagaccg gcgagctctt cccccaggga
    10021 aatgggcggg tgaccgcgcg cgaactggga attccctacc tgggcggcat cccgttcgac
    10081 ccgcggctgg cggatgcggg cgaccgcggc gcgccgtacc tggtcaccta ccgcgacacg
    10141 gccaccggcc aggcaattct caaaatcgcc aagtcggtca cccagttctt tgctgacaaa
    10201 aaccacaaga gttagtattt tccttcaact ggttccttga gaggcgaaac ttcggcctcg
    10261 cgaattcgct ctatgcgtct gctcaatttg atcctggcct ggctgcacct gatctcggcc
    10321 ttcactctgg tcggcggctc gatttttgtc ggcttcgtgc tggcgccggc ggcgaaagct
    10381 cagagcggaa attggattgt gactctggca gccaccgaaa aacgctaccg caagctggcc
    10441 cggcggatgg tggaggtcgt gattctcacg ggcgtggcca acctcctgtt gcatcgtctg
    10501 gccttccaca tgccctggac cggccattat ttaggcacga tcgccctgaa gctggcgttg
    10561 gtgctcacga tggtattcct ctatgcccgg cgggtgaaaa ttctcaccgc actggccgac
    10621 aaaggcacat tgggtgaggt cgaaaacaat gaactgaaac gcgccgacca gcttattggc
    10681 tggcagatgg tcttgggtgc ggtggtgatt cttcttggtc taagtttgcg gggatagcgt
    10741 atttcgcttt agaatacagt atgcagtaca cagcacacgg ggaactactc agataaagaa
    10801 cggatgagag cggtgagcat tcgggaaatt atttccgtct tttgttcggt ggcggcatgg
    10861 tccttcaagt atccgagatc ccgtgaaaga atgagatagt accgaagttc ttccaaagag
    10921 ccctgagaaa tgttgtaaaa atttatttta tcttttgtcc ctcgtttctt aaaaccttcc
    10981 gcaatatttg caggcactga gacggccgcc cggcgcatct gcgacactag accgaatttt
    11041 tcttcacgag gaaattgttt tgtcagcagg tagatctcca gcgctaaccg gtggcttgcc
    11101 tgccagacat tgagctgttg aaaactctct atcttttgca tttcccggtg tgctgtatac
    11161 cgtgtgccgt gtttcgggct taatacagcc gaccgcgttt aatcaaatac gctaataacg
    11221 ctttatcgaa cggcgtggcc gtgtccagct gctggtagtc gatctggctt ttgcggcact
    11281 cgtagaggta gcggtcggtc aacgttttca agcgggcttg atagtctctt ttgatctgcc
    11341 aggggagagt cgtaatctct tcacccgttt ccatatcctt aaagaccgcc tctccgggga
    11401 aggcgaaggt gcgttccagc gggtcgagga tatggaagag aatcacctcg tgcttgcggt
    11461 ggcggaagtg cttgagtccc gagagcaccc ggtcgggatc gtcaaagagg tcggaaagga
    11521 gaataatgag cccgcgcctt ttgatccgct ccgccatttc gtgcagagca gtagcggtgt
    11581 tggtggtctt ctccggcagg gcaccgtcca actcccgcaa aaggacgtgc aggtggtcgc
    11641 gtttggcctt gggtgggatg taacgcttga tcttatcggc ataggtgacc agccccaccg
    11701 aatcgcgctg gaggagcatc agataggaaa gcgccgcggc caggtaggag gcatattcca
    11761 gtttggttat ccgacttttg acctcgggag attgatcctg acttgagtac cccatcgacc
    11821 ccgaagtgtc cagaagcagg taggccttga ggttggtttc ttcctcgaac tctttgatgt
    11881 agtagcggtc cgatttggcg aagactttcc agtcgatgtt gcgcaccggg tctcccggca
    11941 tatactggcg gtgttcggcg aactccaccg aaaagccgtg gtagggggag cggtgcaagc
    12001 cggcgatgaa cccctccacc accagccgcg ccttcagttc catccccttg agtttggaga
    12061 tcacctccgg cactaagaag cggcggtaat cctgcgaggc agcggacggg gtcggcataa
    12121 gggcagaatc tggctcagcg tttcaaaatc aggctgtcgg tctggtcgcg gacgaagccg
    12181 aactgttcat agaaagcatg cttatccctc gtggaaagcc agaagaaggt cacggtcttt
    12241 aacctcggat gggtcaggat gtattccata atttttctac cgatgccctg gctctggtat
    12301 tccggccgga cgatgacatc ccaaatggtt gcccgatagg tgcagtccgt caaaagccgg
    12361 gtgaagccga ccaagaggtc gccgtcccag acggaaatta ccattggcgt gtggtcgagc
    12421 atttttttga catcaggttt tttccgctgg ttgctccacc agccgaactg gtaaaggtcg
    12481 atgagcttgt cgacatcgat gtctttggat tctgaaaagc gaagagaaat tattcctgac
    12541 acagccgtta ggctaaatca cggtttatcc tgtacgtcgg agaggagccg ctggacgatt
    12601 tgctccgttc ccacgccctc ggcttcggca ttgaaactgg tcacaatcct gtgtcgcagg
    12661 accggcggag cgacgaagcg gacgtcttcg gtcgaaggcg tcagccggcc atccaaaatc
    12721 gccttcactt tgccgcccaa gatcaaatac tgcgacgcgc gcgggccggc gccccaagag
    12781 atccagtttt tgatgtactc gtaggtgccg ttctccaccg gccgcgtcgc ccggactaac
    12841 ctcaccgcgt agttaatcac atggtcggcc accggcaccc ggcgggccaa tttctgcaga
    12901 cggatgattt ccacgcccgt cgccacttta tccagactgg cggcataggc ggaggtggtt
    12961 gtccgcacga tttcggcttc ctcggccgag gatgggtagt cgatattgat gttgaacata
    13021 aagcggtcga gttgcgcctc gggcagggga taagtgccct cctgctcaat cgggttctgc
    13081 gttgccaaaa cgaaaaaggg caaaccgagt ttgaaggtct cgccggaggc agtcacctcg
    13141 tactcctgca tcgcctgcag gagagccgcc tgggttttgg gcggggtgcg gttgatctcg
    13201 tcggctaaga cgatattggc aaaaaccggc cccttgacga agcggaagag ccgcttgccg
    13261 gtggtcctgt cctcttccaa tatctcggtg ccggtgatgt cggagggcat caggtcgggg
    13321 gtgaactgga tgcgagaaaa cttgaggtcg agcaccttcg ccagagttga aattaaaagc
    13381 gtcttggcta agccgggaac gccgataatg aggcagtgcc cgccggagag gagggcgatc
    13441 ataagttcgt cgatcacccg ctcctgcccg acgatgactt tgccgatttc ctttttgatt
    13501 atctctttgg cggctttgag tttttcgacc gcctccaaat cgccggtggt tctgactgct
    13561 tcaggcatta gtcctccaaa ttgctcattc taaaagctcg cgaacccagt aatggtacgt
    13621 atcaaaaggc gttctggcaa tggcacaatt tcgagctcat ccgaatggtt tgatggaaag
    13681 gatagatgtt tagtgattca gtggactttt tacttttagg gtgttattga ttaaagacac
    13741 tcgatacgaa aggcgttggc atgatgaaat caatttaatc gctactatac cgactgcccc
    13801 caaccttcac acacgccaat ccctctgaaa aatcccgtgc ttcgttaaaa ctacttttta
    13861 ttacaaactt tcctgtcttg tcgatgtaac cactttttcc accaatttca gcgggagcca
    13921 ccccctcgga aaaattccag gcaaaatcga actgaggact gattgcaaac tttccggttt
    13981 tattgatata acctgatttt gctccaacct ctacacatgc caagccttca gaaaaaggca
    14041 aagcgaaatc gaattctgga ctgatcacca tttttcctgt tgggtaaagt cccattccag
    14101 ccacctatgt gagtctcagc agtcccat
//
LOCUS       DBNGZD010000248         6513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1428416, whole genome shotgun sequence.
ACCESSION   DBNGZD010000248 DBNGZD010000000
VERSION     DBNGZD010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1428416"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /gene="eno"
                     /locus_tag="AC16GM_06560"
     CDS             complement(<1..277)
                     /gene="eno"
                     /locus_tag="AC16GM_06560"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103390.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR2923372.1"
                     /translation="MKEKVQTIKAREILDSRGKPTIEVDCILTNGMVGRAAVPSGAST
                     GAHEALELRDGDTRRYLGKGVLKAVENVNRTIGPALVKKGLDLFNQEA"
     gene            complement(286..1092)
                     /locus_tag="AC16GM_06565"
     CDS             complement(286..1092)
                     /locus_tag="AC16GM_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HIR2923373.1"
                     /translation="MSVERLVWSGLAALVIVSVSLRLRSLSFPGAVAAWVAGSVTYVF
                     GGWAAVVPLLFFFVSGSLLSRWPKRPSKAISLEKPGPRDGWQVLANGGLATVFIVLAF
                     LFPSRNFHLAFLAAYAEAGADTWATEIGLLSRIAPRSIISRKMVSPGTSGGVTALGLL
                     AGFVGALFTAACGFWIQQKEWGWTLGQVALAGTSGFAGSLFDSVLGATVQQKFRCPDC
                     GQMTERRECRCGGKTVFASGIRGLNNDWVNFLSTFFSAGFALLLVGLFAY"
     gene            complement(1102..3300)
                     /locus_tag="AC16GM_06570"
     CDS             complement(1102..3300)
                     /locus_tag="AC16GM_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923374.1"
                     /translation="MWPGSWSDFTVALLERKGYLVFGGIALYSAAVFFYYRRTLPPLN
                     VKILRLLGGVRWLAVAAVFAGLSEPLVSYEKVSRLPPTVAVLVDASRSMAFREGEKTR
                     REQALELAGQPWIDELKRKVVVVSYDFADSLLPFPPAAMGRGSAAQAASGASTNIGRA
                     LRSLGQKYREQNLSGVILLSDGANNAGPDPVGAAWQSGFPIYPCAVGADVVPADVSVD
                     QVRAPEVAYAKEKVPVTISVSSAGFEGKRIPVEIRFDKKVVTRTLLTLAGQGKKQEVS
                     LELAADSAGLFTYRVSVPLQAGERLRVNNERQFVLKVLPNKIRVGILAAFPSWEYRFL
                     VQALEQSGGFEVASYFTNAPPVNASGFRWSEPALAQADLLLLLDPPGNFLGGKEARLR
                     KFVEQEGKGLVLFFGPQLVSSWRAVDQATFPFVSGLIAEQSGTFGLELTVDGKRNGLT
                     RLVANDEENEKLWTTLPPLLQVVAVQKSWLGGVPLVTATDQRSGQTIPVVTVGPWGQG
                     KVFLVTGSPLWRWSFFAADLNRYPESYALFWKNCLQWLSTKDDLNRVRVSTDRLVYKA
                     GEEVLITGSVFDERYQPLAGAQVKLQAKNLDSNATTATAELFEEESGQYQTALRALAP
                     GRYQLILAAAKEGKQVGEAAARFTVEQFGLEDENLSVDRGLLEELARASGGKFYTPAT
                     LAQIVNDLQPQAESMAQKREVEVWNSPLLFLAIVLLLGTEWALRKRYQLL"
     gene            complement(3400..4422)
                     /locus_tag="AC16GM_06575"
     CDS             complement(3400..4422)
                     /locus_tag="AC16GM_06575"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR2923375.1"
                     /translation="MTPSITAALDPLTEIFSPIQSDLEAFERLLERSLVSDADLITEI
                     NRHLLARRGKRLRPAIVFLAARLCDVGRGTAAAPFSLRAGYAALAIELIHTATLLHDD
                     VVDESPTRRGQATVNAKWKNLIAVLMGDYYFSKAFTLLVKAETEGLMGAVSTATERVS
                     VGQLLEIQESRNFGVTEAAYFRIIQEKTASLFGAAAEAGALVAGAPLAAQLALREYGE
                     KLGNAFQIADDGLDLVGKVEKTGKDLGTDLREGKVTLPLIFALAQAADGNRERIVQLL
                     ENGMVDGDFSEVVSFIRTQGGLEYASRKARTFGSEAQALLASFPDSAYRQALSLLARF
                     AVERER"
     gene            complement(4467..5267)
                     /locus_tag="AC16GM_06580"
     CDS             complement(4467..5267)
                     /locus_tag="AC16GM_06580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2923376.1"
                     /translation="MKKRSQTLIERAVPDIFSLEGKVAIVTGGSRGIGRETALLLAAF
                     GAEVVVHYHRKRRAAEEVQRQIWSQGRSAEIYGADCSSYAQCRRLVNFTQKRFGRIDI
                     LVNNAAIWEEAPIHRLTPQQLEKTLAVNLKSIFYCTRLAAPIMMRQKSGSIVNLSSTA
                     GQRGEAFHSHYAATKGAITSLTKSLAAELAPYRIRVNAVAPGWVDTDMSHGALTGRDR
                     KKILGAIPLGRAAQPREIAACIAFVASEAAAFVTGEIFNVNGGAVLCG"
     gene            complement(5264..>6513)
                     /gene="guaA"
                     /locus_tag="AC16GM_06585"
     CDS             complement(5264..>6513)
                     /gene="guaA"
                     /locus_tag="AC16GM_06585"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR2923377.1"
                     /translation="ARTHAREYGRAELRIKTFGKLFAGLQPKSVVWMSHGDTVVKLPP
                     GFAGLATTPQVKFAAFGHAAEKIYAVQFHPEVAHTEGGQRILRNFVLTICGARGDWTV
                     RSFISESTARIQATADSLPVVCGISGGVDSTVCALLIHRAIGKNLIPIFIDNGLLRAG
                     EAAEVQKLFARLKIPLRTVDASQRFLSALVGVSEPETKRKIIGHVFIETFAHATRQLG
                     EVKFLAQGTLYPDVIESQSVRGPSATIKTHHNVGGLPADLKFQLIEPLRNLFKDEVRA
                     VGRKLGVPAQSLGRHPFPGPGLAVRIIGEVTPERLELLRRADFIFIQELRKAKLYDQI
                     WQAFVVLLPVKTVGVMGDERTYENVCALRAVTSRDGMTADWARLPDAFLARVSARIIN
                     EVKGINRVVYDISSKPPATIEWE"
BASE COUNT         1326 a         2073 c         1902 g         1212 t
ORIGIN      
        1 ccgcttcctg attgaagagg tccaatccct ttttcaccaa tgcggggccg atggtgcggt
       61 tgacgttttc gaccgctttc aaaacgccct tacccagata gcgccgcgta tcgccgtcgc
      121 gcagttccag cgcctcgtgc gctccggtgg aggcgccgga gggcaccgcc gcgcggccca
      181 ccatgccgtt ggtaaggata caatcgactt cgatggtggg tttcccccgc gaatccagaa
      241 tctcgcgggc tttgatggtt tggacttttt ccttcatcgg cgcgttcaat atgcaaaaag
      301 ccctaccaaa agcaatgcga aaccggcgga aaagaaagtg gaaaggaagt tgacccagtc
      361 gttattcagc ccgcgaatcc cggaggcaaa taccgtcttc ccgccgcaac ggcactccct
      421 gcgctccgtc atttgcccgc agtcagggca gcggaatttc tgctggacgg tggcacccag
      481 aactgaatcg aaaagacttc ccgcaaagcc gctggttccg gccagtgcca cctgcccgag
      541 tgtccatccc cactctttct gctgaatcca gaagccacaa gcagcggtaa aaagcgctcc
      601 cacaaaaccg gccaacaatc ccagcgcggt aacaccgcct gaggttcccg gagataccat
      661 cttgcgcgaa ataattgagc ggggtgcgat ccgagacagg aggccgattt cggtcgccca
      721 ggtatcggcg ccggcttcgg cgtaagccgc caggaacgcc aaatgaaaat tgcgagaggg
      781 aaaaagaaac gcgagcacga tgaagacggt cgctaggccg ccgttggcca aaacctgcca
      841 gccgtcgcgc ggtcccggct tttccagtga gattgctttc gaagggcgct tcggccagcg
      901 ggaaagcagg ctgccggaaa cgaagaaaaa aaggagcggc accacagccg cccacccccc
      961 gaaaacgtag gtgacagaac ccgcaaccca ggcggccact gctcccggga acgaaagcga
     1021 gcggagacgg agtgaaaccg agacgatgac cagcgcggcc agcccgctcc agaccaagcg
     1081 ctcgacgctc accggctcag actataacag ttgataccgt ttgcgcagcg cccattcagt
     1141 tcccagcaag aggacgatgg ccaggaataa cagcggagaa ttccagacct cgacttccct
     1201 tttctgcgcc atcgactcgg cctgcggctg gaggtcgttc acgatctgcg ccaaagtcgc
     1261 cggtgtgtag aacttcccgc cgctggcgcg ggcgagctct tccaaaagcc cccggtcaac
     1321 cgagaggttt tcatcttcca atccgaactg ctccacggta aagcgcgccg cagcctcccc
     1381 cacctgcttc ccctccttgg cagccgcaag gatgagctga taccgtcccg gagccagcgc
     1441 ccgcagcgcc gtctgatact ggccggactc ttcttcaaaa agctctgcgg tcgccgtcgt
     1501 ggcgttgctg tcaaggtttt tcgcctggag tttgacctgt gccccggcca gaggttgata
     1561 ccgctcgtcg aagaccgaac cggttatcag gacctcctca cccgctttgt aaaccaatcg
     1621 gtcggtggag acgcgcaccc ggttcaggtc gtctttggtc gaaagccact gcaggcaatt
     1681 cttccaaaac agagcgtaac tctccgggta ccggttaagg tcggcggcaa aaaaggacca
     1741 gcgccagaga ggtgacccgg taaccaaaaa caccttgccc tgtccccacg gtcccacggt
     1801 gacgaccgga atggtttggc cggaacgctg gtcggtcgct gtcaccagcg gcactcctcc
     1861 cagccagctc ttctgcaccg ccaccacctg caaaagcggc ggcagtgttg tccagagttt
     1921 ctcgttttcc tcatcgttgg cgaccaaacg cgtcagaccg ttacgcttcc cgtcgacggt
     1981 aagttccaag ccaaaggttc cggattgctc cgctatcaag ccggagacga agggaaacgt
     2041 cgcttgatcg accgctctcc aggaagaaac gagctggggg ccaaagaaaa gtaccagccc
     2101 tttgccctcc tgttcgacga acttcctcaa acgcgcttct ttcccgccca gaaaatttcc
     2161 cggaggatcg agcaacagca gaagatcggc ttgggccagt gccggctcgc tccagcggaa
     2221 accggaggcg ttgaccggcg gcgcgttcgt gaaatacgag gcgacttcaa atccgccgct
     2281 ctgctccagc gcctgcacca ggaaccggta ttcccacgaa ggaaaagcgg cgaggatgcc
     2341 gacccgaatt ttgttcggca gcactttgag aacgaactga cgctcgttat tcactctcaa
     2401 ccgctcgccc gcctggagcg gaacgctcac ccggtaggtg aagagtccgg ccgaatcggc
     2461 ggcgagctcc agggaaactt cctgcttctt cccctgtccg gctaaggtga gcagtgttcg
     2521 ggtaacgact tttttatcaa agcgaatctc gaccggtatg cgctttcctt cgaacccggc
     2581 actggagacc gatatggtga ccggcacttt ctctttcgcg taggcaacct ccggcgcccg
     2641 aacttggtcc acgctgacat ccgccggtac cacgtcggct cccaccgcgc aggggtaaat
     2701 cggaaaaccg ctctgccacg ccgctcccac cgggtccggc cccgcattat tggccccgtc
     2761 ggagagcaag atgactccgg agagattttg ttcccggtac ttctgaccca gtgagcgcag
     2821 agctctcccg atgttggtgc tggcgccgct ggcggcctgc gcggccgaac cacgccccat
     2881 agcagcgggc gggaaaggca aaagcgagtc ggcgaaatca taagagacga ctaccacttt
     2941 tcttttgagt tcatcgatcc agggctgccc ggcgagttcc agcgcctgct cccggcgggt
     3001 cttttcccct tcgcggaagg ccatgctgcg tgaggcgtcg acgaggacgg ccacagtcgg
     3061 tggcaggcgg gaaactttct cgtagctgac caggggctcg ctcaacccgg caaagaccgc
     3121 cgccaccgcc agccagcgca ctccccccaa taaccggaga attttgacat ttaaaggcgg
     3181 cagcgtgcgc cggtagtaaa agaacaccgc cgccgagtag agggctatcc cgccaaaaac
     3241 gaggtagccc ttccgttcca aaagtgccac cgtaaaatca gaccagctac ccggccacat
     3301 cctcttcctg tagtacgata gattctttcc ggcaacgctg catcgtggat acaaaaaaac
     3361 caccgccagg gcagtggctg gaaccagaac agtttgcgct taacgctccc tctcgaccgc
     3421 aaagcgcgcc aaaagggaga gcgcctggcg gtacgccgaa tccgggaaag aggccaaaag
     3481 cgcctgggcc tcgctcccga aggtgcgcgc cttccgcgaa gcgtactcca gccctccctg
     3541 ggttcggatg aaactcacta cctcggagaa gtccccgtca accatcccgt tttctaagag
     3601 ctgcacaatg cgctcccggt tgccgtccgc ggcctgagcc agagcgaaaa tgagcgggag
     3661 ggtcaccttt ccttcccgca ggtcggttcc cagatccttg ccggtttttt ccaccttgcc
     3721 gaccagatcc aggccgtcgt cggcgatctg aaaagcgttt cccaattttt ctccatactc
     3781 ccggagcgcc agttgcgctg ccagcggtgc gccagccacg agagcccccg cttcggcggc
     3841 ggcgccgaag agtgaggcgg tcttctcctg aatgatccgg aagtaggcgg cttcggtcac
     3901 gccgaaattc cggctctcct gaatttccaa aagctgcccc accgacaccc gctcggtggc
     3961 ggtggagacc gcgcccatga gaccttcggt ttctgctttc actaaaaggg tgaaggcttt
     4021 ggagaagtag tagtcgccca tgagcactgc gatgagattt ttccacttgg cgttcaccgt
     4081 ggcctggccc cgtcgggtcg gagattcgtc caccacgtcg tcgtgcaaaa gcgtggcagt
     4141 gtgaatcagt tcaatggcca aggccgcgta ccccgcgcgc agggaaaagg gcgctgccgc
     4201 cgtcccgcgc ccaacgtcgc agagccgcgc cgccagaaaa acgatcgccg gccgcagcct
     4261 ttttccccgg cgtgccagaa ggtgccggtt gatttcggtg atcaggtcgg cgtcacttac
     4321 gagcgaccgc tccaggaggc gttcaaacgc ttccagatca gactgaatcg gagaaaaaat
     4381 ctcagtcagg ggatccaggg cggcggtgat ggaaggcgtc atgacggagg aaggtacgcg
     4441 gtgtgcgggg actgtcaagg ggtgcctcac ccgcacagaa ccgcaccgcc gttgacgttg
     4501 aaaatctcgc cggtgacgaa ggcggccgcc tccgaagcca caaaagcgat acaggcggcg
     4561 atctcccgcg gctgggcggc gcgtcccaag ggaattgccc ccagaatttt tttccggtcc
     4621 cgacctgtga gcgctccgtg gctcatgtcg gtatcgaccc agcccggtgc caccgcgttc
     4681 acccggatgc ggtagggcgc cagttccgcg gccaaagact tggtcaaact cgtgatcgcc
     4741 cctttcgttg cggcgtagtg ggagtggaac gcttcgcccc gctgtccggc ggtggaggaa
     4801 agattgacaa tggagccgga tttctggcgc atcatgatgg gagccgccag ccgcgtgcag
     4861 tagaaaatac ttttgagatt aaccgccaaa gtcttctcca actgttgcgg cgtcaggcgg
     4921 tgaatcgggg cctcttccca gatagcggcg ttattgacca gaatatcgat gcggccgaaa
     4981 cgtttttggg tgaagttgac cagccgtcgg cattgggcgt aggacgaaca atcggcgccg
     5041 tagatctccg cgcttctacc ctgcgaccaa atctgccttt gcacttcttc agcagcccgc
     5101 cgtttccggt ggtagtgaac caccacctcg gcgccgaacg ccgccaggag gagggcggtt
     5161 tcccgtccaa tcccccgcga tcctcccgtg acgatggcga ctttcccctc cagtgaaaaa
     5221 atgtccggta ccgctcgctc aataagcgtc tgcgatcttt ttttcattcc cactcgatcg
     5281 tcgcgggcgg cttggaagaa atgtcgtaca ccacccggtt gatgcctttc acttcgttga
     5341 tgattcgggc ggacacccgc gccagaaaag catccggcag gcgcgcccaa tcggcggtca
     5401 tgccgtcccg gctcgtcacc gcccgcaacg cgcagacgtt ctcgtaggtg cgctcatccc
     5461 ccatcacgcc cacggttttg acgggcaaaa ggacgacgaa ggcctgccag atctggtcgt
     5521 agagcttcgc cttccggagt tcctggatga agatgaaatc ggcgcggcgc aaaagttcta
     5581 agcgttccgg cgtcacttct ccgatgatcc ggaccgccag gccgggcccg ggaaaggggt
     5641 ggcggcccag ggactgcgcg ggcaccccta gcttccgccc caccgcgcgt acttcgtctt
     5701 tgaacaggtt gcgcaacggt tcgattaatt gaaatttgag atccgccggc agcccgccga
     5761 cgttgtggtg cgtcttaatg gtggccgacg gtccgcgcac gctctggctc tcgatcacgt
     5821 ccggatagag cgtcccctgc gctagaaact tcacctcccc cagctggcgc gtggcgtggg
     5881 cgaacgtttc gatgaagacg tggccgatga tcttgcgctt ggtttccggc tcgcttaccc
     5941 cgaccagggc ggaaagaaag cgctgcgacg catccacggt gcgcagtgga atcttcaacc
     6001 gggcaaagag cttttggact tcggctgctt cgccggcccg caaaaggccg ttgtcgatga
     6061 aaatcggaat gaggttcttg ccgatcgccc ggtggattaa aagcgcgcag acggtggaat
     6121 cgacgccgcc cgaaatgccg cagaccaccg gcaggctgtc cgcggtcgcc tgaatccgcg
     6181 cggtactttc gctgatgaag gagcggacgg tccagtcgcc ccgggcccca cagatggtca
     6241 gaacgaaatt gcgcaggatg cgctgccccc cttccgtgtg ggccacctcg gggtggaact
     6301 gcacggcgta gattttctca gccgcgtggc cgaaggcggc gaacttcacc tgtggcgtcg
     6361 tggccaggcc ggcaaacccg ggcgggagtt tgaccaccgt atcgccgtga ctcatccaga
     6421 cgacgctttt gggctgcagg ccggcgaaca gtttcccgaa ggttttaatc cgcagttcgg
     6481 cccggccgta ctcgcgggcg tgggtgcggg cga
//
LOCUS       DBNGZD010000249         2551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1428521, whole genome shotgun sequence.
ACCESSION   DBNGZD010000249 DBNGZD010000000
VERSION     DBNGZD010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1428521"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..172
                     /locus_tag="AC16GM_06590"
     CDS             2..172
                     /locus_tag="AC16GM_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR2923378.1"
                     /translation="MLNDNLEGELRAIARYKKQIASAAVESEPAIRHMLEEILEDEED
                     HADIWQTMLEGL"
     gene            complement(229..1209)
                     /gene="thiL"
                     /locus_tag="AC16GM_06595"
     CDS             complement(229..1209)
                     /gene="thiL"
                     /locus_tag="AC16GM_06595"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR2923379.1"
                     /translation="MPVGEFELIDFIRKKFAPSKKRGAVVLGVGDDAAALRLAGDKLL
                     LVTTDTVVEDVHFKLRYFTYEQVGHRIMAANLSDIAAMGGWPTYYLVTIGLPKGTTQK
                     NVDAIYRGMERLARKHRVALIGGDIVRAEKLFVTVTLLGEVERKNLLTRSGAHRGNLV
                     CVTGHLGASLLGFRLLNQKSAKRVPHRLTRRHLQPEARLAEARWIVQNLRPTAMIDIS
                     DGLSSELNHLARESRVGFAVEAGKIPVPPGVRRAIGNLKGNWVDYALASGEEYELLFT
                     VPKSQVARLKNWPKHLAPVSVIGRVAAKGVVLIGPEGRVEKIRPSGWRHL"
     gene            complement(1266..>2551)
                     /locus_tag="AC16GM_06600"
     CDS             complement(1266..>2551)
                     /locus_tag="AC16GM_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR2923380.1"
                     /translation="NHQWGDPITTSDGLPDNSVLELAYDPTQDALWAATRLGTASFQS
                     TLRQWYLAPRFPPDVKPKSKPPQNPLPNFYIDFNYTLYPEGVIQDQFLRRYPVRTSLE
                     DDMGSIWYGTWGLGVLEGTPRDQRATLRHFGLWSSVVTAMAIDGDFVWFGGTRAFTQP
                     SGITRWNRQTQEWTYYEAPYTDGLISDNINAIVADSQYVWFGTELGLVRYDKKRNRFK
                     SYTRRQRVADEEVTALDVDSNYLYVGTTNGVSILDKKKDSLWVENAGLPPATVVNAIK
                     STGDDLWIGTRDGLFRKTKGQPKWVRYLDPTRILSAEVRAIVRAGDELWFGSQIGVVR
                     FNLVTQKTSAFEQGAGTGRLQVYGLAVDSLQAFIAVPGGVWRWQRGGDFWRLLSTNDG
                     LIDPTVYQMVWDGDYLWFATNKGATRFYWKTRRIE"
BASE COUNT          540 a          768 c          719 g          524 t
ORIGIN      
        1 gatgctcaac gataatctag agggcgagtt aagagcgatt gcccgctaca agaagcagat
       61 tgcctccgcg gccgtagaga gcgagccggc catccgccat atgttggagg aaatcttgga
      121 ggacgaagag gaccacgccg acatctggca gacgatgttg gaagggctgt gagcgatcat
      181 ccataccaac ggatccggga ggggcgagtt cactcgccct ttttttgttt acaaatgccg
      241 ccagccgctc ggtcgaattt tttccacgcg gccttccgga ccaataagta cgactccttt
      301 agctgccacc cggcctatga cggaaacagg cgccagatgc tttggccagt tcttgagccg
      361 ggcaacttgt gatttcggaa cggtaaaaag cagttcatac tcctcgcctg aagccagcgc
      421 gtagtcaacc cagttccctt tcaaatttcc gattgcccgc cggacaccgg gcggcaccgg
      481 aatttttccc gcctctacgg caaatccaac ccggctctcc cgcgccagat ggttgagttc
      541 gctggagagc ccgtcggaaa tgtcaatcat ggccgtaggc cggaggtttt gcacaatcca
      601 gcgagcttcg gctaaacggg cctctggctg caagtgtctc cgggtcaaac gatgcggcac
      661 tcttttagct gacttttgat tgagcaaccg aaaccctaag agagacgcac ctaagtggcc
      721 ggtgacgcag accagattgc ccctgtgcgc tccggaacgc gtgagcaaat tcttccgttc
      781 aacttcgcct aaaagcgtca ccgtcacgaa aagtttctct gcccgcacaa tatccccgcc
      841 gatgagcgcc acccgatgtt tgcgagcaag acgctccatc ccgcggtaga ttgcatcgac
      901 gtttttttgc gtcgtccctt ttggcaaacc gattgttacc aggtaatagg tcggccaccc
      961 gcccattgcg gcaatgtcgg agagattggc cgccataatc cggtgcccga cctgctcata
     1021 ggtgaagtag cggagtttga agtggacatc ttccaccacc gtgtcggtgg tgactaagag
     1081 gagtttgtct ccggctaaac gcagcgccgc cgcgtcatcc cccacgccca aaacgaccgc
     1141 cccgcgcttt ttggaagggg caaacttttt gcggatgaaa tcaatcagtt caaattcgcc
     1201 caccggcata aagtaaaaat accgctgccg gacaaacttt gaaagtcaaa cttttgtacc
     1261 tgagtttact ctatccgccg cgttttccag tagaaccgcg ttgcgccctt gttcgtcgca
     1321 aaccagaggt aatcgccatc ccagaccatc tggtagacgg tcgggtcgat tagaccatca
     1381 ttcgtggaaa gcaaccgcca gaagtcgccg ccccgctgcc agcgccagac cccgcccggc
     1441 accgcgatga aggcctggag cgaatcgacc gccagcccgt acacctgcag ccgtccggtg
     1501 cccgccccct gctcaaacgc ggaggttttc tgcgtcacga gattgaaccg caccaccccg
     1561 atctgcgagc cgaaccacaa ctcgtccccc gcacgcacaa tggcgcgcac ttcggcggag
     1621 agaatccggg tcggatcgag gtagcgcacc cacttgggtt ggcctttcgt cttacggaag
     1681 aggccgtcgc gggtaccgat ccagaggtcg tcgccggtcg atttgatggc attaaccaca
     1741 gtcgccgggg gcaaaccggc gttctccacc cagagactgt ctttcttttt gtccaaaatc
     1801 gagacgccgt tcgtcgtccc gacgtagagg taattgctgt ccacgtctaa agcggtcact
     1861 tcctcatcgg caactcgctg ccggcgggtg taacttttga accggttcct tttcttgtcg
     1921 taccgcacca ggcccagctc ggtgccgaac cagacgtatt gggaatcggc cacaatcgcg
     1981 ttgatgttgt cggagatgag gccgtcggtg tacggcgcct cgtagtaggt ccactcctgt
     2041 gtttgacggt tccagcgggt tatgccgctg ggttgggtaa aagcccgcgt accgccgaac
     2101 cagacaaaat cgccgtcaat ggccatggcc gtcacgaccg acgaccagag cccaaaatgt
     2161 cgcagcgtgg cccgctggtc gcgcggggtt ccctccagaa cgcccagtcc ccaggtgccg
     2221 taccagatgg aacccatgtc atcctcgagc gaagtgcgga ccggatagcg ccggagaaac
     2281 tggtcctgaa tcaccccctc aggatagagg gtgtagttga aatcgatgta aaagttcggc
     2341 agaggatttt ggggcggctt gcttttcggt ttcacatccg gaggaaagcg cggggcgaga
     2401 taccactggc gaagggtact ctggaaggag gcggtcccca ggcgggtggc cgcccacaag
     2461 gcatcctgcg tcggatcgta ggccagttcc agaacgctgt tgtccggcaa gccgtcggag
     2521 gtggtgatcg ggtcgcccca ctggtggtta a
//
LOCUS       DBNGZD010000250         2095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_178064, whole genome shotgun sequence.
ACCESSION   DBNGZD010000250 DBNGZD010000000
VERSION     DBNGZD010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_178064"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="lnt"
                     /locus_tag="AC16GM_06605"
     CDS             complement(<1..1067)
                     /gene="lnt"
                     /locus_tag="AC16GM_06605"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR2923381.1"
                     /translation="MNKRWTPSARFEFGLVVGSALLLTLAYPPFPFGFLAYFALAGLL
                     WAIEGKSVAQAFRFTFLFGFVNHGTLLYWVGWAVRPQILGALGAVAAVLVLSLYLAIT
                     VGLFQFVRRRLVGIDPYRGSWAIVLFPFFWVAHEYLRSLGELGFPWTNLSYTQTYYPH
                     LIQYASFTGDLGVSFWIVLVNVAVFGLWRFWTKRRTMLRLAMGLAILFLTPFVYGYLV
                     IPRKTAGSGVPVALLQANIDSNLKWDQAYTASSFAIFDTLAGEATAKGARLLIWPDSS
                     APCYLRAEPVWLLHLQRQAAELKKPMLVGALDYESPAAGKYIFYNAAFQFDSLGNLSP
                     PYRKIHLVTVGERIPFRDEVTV"
     gene            complement(1132..>2095)
                     /gene="gpmI"
                     /locus_tag="AC16GM_06610"
     CDS             complement(1132..>2095)
                     /gene="gpmI"
                     /locus_tag="AC16GM_06610"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR2923382.1"
                     /translation="DKRWDRTTGAYLAMVKGEGKIASDPVRAVEESYQANVTDEFVAP
                     VVITENGDPREGRIADGDEVIFFNFRADRTRQISHLLVDQDRPVAGVNPKPEVNLTAM
                     THYDDTLKVPEAFPAQFVTNTFGELVSRAGLKQLRIAETEKYPHVTFFFNGGNEAPFP
                     GEERILIPSPKVATYDLQPEMSAPEVTRKVVEKIGAGEFDVIILNYANPDMVGHTGVL
                     PAAIKAVETVDGCLAQVLRAVEEVNDSAILTADHGNCDLMVDAKTGGPHTAHTTNPVP
                     CAVIHSGFSGKLRAGGILADVAPTLLEMLHLQPAPEMTGKSLLH"
BASE COUNT          472 a          642 c          575 g          406 t
ORIGIN      
        1 accgtgacct cgtcccggaa aggaattcgt tctccgaccg tcactaaatg tatcttccgg
       61 tagggcggcg acagattccc cagcgaatcg aactgaaagg cggcattgta aaagatgtat
      121 ttccccgcag ccgggctttc ataatccagc gcgcccacta acattggctt tttcagttca
      181 gccgcctgcc gctggaggtg caaaagccag accggctccg cccgcaggta acagggcgcc
      241 gacgagtcgg gccagatgag caaacgcgcc cccttggctg tcgcctcccc ggccagagtg
      301 tcgaagatgg cgaaacttga ggcggtgtag gcctgatccc atttgaggtt cgaatcgatg
      361 ttcgcctgca ggagcgccac cggtacgccg gacccagcgg tcttccgggg aatcacgaga
      421 tagccgtaca cgaagggcgt caaaaaaaga atcgccagtc ccattgccag acggagcatc
      481 gtccgccgct tggtccagaa acgccaaaga ccaaacaccg ccacattcac cagcacaatc
      541 cagaaagaga cgcccagatc gccggtgaaa ctggcgtact ggattaggtg ggggtagtac
      601 gtttgcgtat aactcaagtt cgtccaggga aatcccagtt ctcccaacga gcgcaggtat
      661 tcgtgcgcca cccagaagaa agggaaaaga acaatagccc aactgcccct gtacgggtcg
      721 attccgacca gccgccggcg gacgaactga aacaagccga cggtgattgc taaatagagc
      781 gacagcacca gcaccgccgc caccgctcct aaagcgccga gaatctgcgg ccgcactgcc
      841 cagccgaccc agtagagcag cgtcccatga ttgacgaacc cgaataagaa agtgaatcga
      901 aatgcttgag caaccgactt tccctctatc gcccagagca atccggccag ggcaaagtag
      961 gccagaaacc cgaagggaaa cggcgggtag gccagagtca aaaggagcgc cgagccgaca
     1021 accagcccga attcaaaacg ggcggacggc gtccatctct tgttcaacac tgctgcggta
     1081 tgtgattctt tcacaggaat tgcgacggtt tgactcaaaa tccaaatctc cttaatggag
     1141 cagacttttt cccgtcatct ccggtgccgg ttgcagatgg agcatttcta aaagcgtcgg
     1201 cgccacgtcg gccagaatgc ctccagcgcg caatttcccg gaaaatcccg aatgaatgac
     1261 cgcacagggc acgggattcg ttgtatgcgc cgtatgcggg ccgccagtct tcgcatccac
     1321 cattaggtcg cagttgccgt gatcggcggt gagaatggcg gagtcattga cttcctccac
     1381 tgcgcgcaga acttgcgcga ggcacccatc caccgtttct accgctttga tggccgctgg
     1441 aagtacgccg gtgtgcccca ccatatccgg gttggcgtag ttcaggatga tgacgtcgaa
     1501 ctccccggcc ccgatttttt ccaccacctt ccgggtcacc tccggcgcgc tcatctccgg
     1561 ctgtaagtca tacgtcgcca ctttgggcga agggatcaaa atccgctcct cgcccggaaa
     1621 gggcgcttcg ttcccgccgt tgaaaaagaa ggtgacgtgc ggatatttct ccgtctcggc
     1681 aatgcgtaac tgcttcagcc cggcccggct cactaattcc ccgaaagtat tggttacgaa
     1741 ctgagctggg aaggcctcgg gtactttgag cgtgtcgtcg tagtgcgtca tggcggtgag
     1801 attgacctca ggcttcggat taactccggc gacgggacga tcctgatcca ccaaaaggtg
     1861 cgagatctgc cgcgtccgat cggcccggaa attaaagaag atcacttcgt cgccgtcggc
     1921 tatgcgtcct tcccgcggat ccccgttttc ggttatcacg accggcgcca cgaactcgtc
     1981 ggtcacgttg gcctggtacg attcctccac cgcgcgcacc gggtcggaag cgattttccc
     2041 ctcacctttg accattgcta agtacgcacc ggtggttcga tcccagcgct tgtcc
//
LOCUS       DBNGZD010000251         1959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_109243, whole genome shotgun sequence.
ACCESSION   DBNGZD010000251 DBNGZD010000000
VERSION     DBNGZD010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_109243"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="AC16GM_06615"
     CDS             complement(<1..370)
                     /locus_tag="AC16GM_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR2923383.1"
                     /translation="MNPYYKIGWRLSRFVGKLLWSFRAEGLEHVPPTGGMIIASNHRS
                     YADPPLVGAAILRQLFFFAKAELFTMPVLGWLVRHLNVLPIKRQRYDREGIERALEVL
                     RAGNGLVVFPEGTRCRTDDFL"
     gene            complement(367..1071)
                     /gene="cmk"
                     /locus_tag="AC16GM_06620"
     CDS             complement(367..1071)
                     /gene="cmk"
                     /locus_tag="AC16GM_06620"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR2923384.1"
                     /translation="MPRHSRGKIIAIDGPAASGKSTTARLVAKKLGFLWLDTGAMYRA
                     VTLFALEQSVPGDNEPALAALARRLRFEFRAQHNGSRLWVNGRDVSQGIRSLFVTRAV
                     SQVSKHPQVRRVLVKQQQVCGRRGNIVVEGRDTTTVVFPRADLKIYLDASLKERARRR
                     LAELREAGVKSTLAEQMKAITLRDRSDSRRQASPLRRAPDAVYLDTTKLTVRQQVTAI
                     VRLFRQKFPGGTKRKI"
     gene            complement(1385..>1959)
                     /locus_tag="AC16GM_06625"
     CDS             complement(1385..>1959)
                     /locus_tag="AC16GM_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2923385.1"
                     /translation="QSALNRRELSRRGLAPRGSWAERGLGTTILGAHLGIPAGDFSNV
                     YRVSTGFSVWRNLWVKFRLGWLEVLVGYQKFLRKQKPRGSLYIYPITGNVVLRLPDAL
                     FRPYLTLGGGWYWWQSQVSAPGDSLQYLASGRGLGTSVGVGVEYYLKTKVALDVGLRY
                     HSSPGPGAKAGLNSSRLRFLTLWIGHYFRF"
BASE COUNT          411 a          591 c          588 g          369 t
ORIGIN      
        1 ggagaaaatc gtcggtgcgg cagcgggtgc cttcggggaa aacgaccagg ccgtttccgg
       61 cccggagcac ttccagcgcc cgctcaatcc cctcccggtc gtagcgctgg cgtttgattg
      121 gcaaaacatt caagtgccgg acgagccagc ccaaaacggg catggtgaat aattcggcct
      181 tggcaaaaaa gaacaactgg cggagaatgg ccgcgcccac caggggcggg tcggcgtagg
      241 agcggtggtt ggaggcaatg atcattccgc ccgtaggggg aacatgttcc agcccctcgg
      301 cacgaaaact ccagagtagt tttcccacaa agcggctgag ccgccagccg attttgtaat
      361 acgggttcat atcttgcgtt ttgtcccgcc tggaaatttt tgtcggaata atctgacgat
      421 tgccgtcacc tgctggcgca ccgtcagctt cgtcgtatcg agataaacgg catccggtgc
      481 ccggcggagt ggactggctt ggcgccggct gtcgctgcgg tcacgcagag taatcgcttt
      541 catttgctcc gccagagtgc ttttgacgcc cgcctctcgc agttcggcca gtcgtctgcg
      601 ggctcgctct ttgagcgagg catcaaggta gattttcaaa tcggcccgcg ggaaaacgac
      661 ggtcgtcgtg tcgcgccctt cgacgacgat attccctctg cgaccacaga cctgctgctg
      721 tttcaccaga accctgcgca cctgcgggtg cttcgaaacc tgggacacgg cgcgcgtcac
      781 gaaaagcgag cggataccct ggctgacgtc ccgaccattc acccaaagac gagagccatt
      841 gtgctgtgcg cgaaactcaa accgcaaacg ccgcgccagt gcagccaaag cgggttcgtt
      901 gtccccaggg accgactgct ccagcgcgaa gagcgtgacc gcgcggtaca tcgcgccggt
      961 gtcgagccag agaaagccca attttttggc caccagccgg gcggtggtgc ttttccccga
     1021 cgccgccggg ccgtcaatgg cgatgatttt gccgcgtgaa tgtctgggca tcggatcaaa
     1081 aagataaaca gagatgcgtt agcggccaac ggcgcaaacc gcaattggcg actaaatttc
     1141 aaatttccat aatggctgct taagtctatg accggctcag ggacgagccg gtttacctcg
     1201 gatcggtagt cgggctcgtc tcagagcccg acttcaaaac tcaaaggtaa gtctaaaaac
     1261 ctcggtacaa atcgttccga cccgccaacg ccggtcgggt ctttctcttt ttcgcgcagg
     1321 cggttcatcc tgaacctgcc gaaggacact tcagtgccgc cggttgttgc ctgaccccga
     1381 cgtatcagaa ccgaaagtag tggccgatcc agagggtgag gaagcggagc cgacttgaat
     1441 tgagcccggc tttggcaccc ggtcccgggg aggagtggta gcggagtccc acgtccagtg
     1501 cgaccttcgt tttcaagtaa tattcaacgc cgacgcccac cgacgtcccc aggccccgtc
     1561 ccgaagccag gtactgcagg ctgtcgccgg gcgcgctcac ctgtgactgc caccagtacc
     1621 agccgccacc gagagtgagg tagggacgaa aaagtgcatc gggcagacgc agcacgacgt
     1681 tgccggtgat cgggtagatg tagagcgacc cgcgcggctt ttgcttgcgg agaaatttct
     1741 ggtagccaac cagaacctcg agccaaccca gacggaattt gacccagagg ttgcgccaga
     1801 ctgaaaaccc ggtggagacg cggtagacgt tggagaaatc ccccgcggga atgcctaaat
     1861 gggcgcccag aattgtggtc cccaatcccc tctccgccca tgagccacgc ggcgccaagc
     1921 cgcggcggga gagttcccgc cggttgagcg cgctctggg
//
LOCUS       DBNGZD010000252         9662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_663765, whole genome shotgun sequence.
ACCESSION   DBNGZD010000252 DBNGZD010000000
VERSION     DBNGZD010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_663765"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="AC16GM_06630"
     CDS             complement(<1..1449)
                     /locus_tag="AC16GM_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923386.1"
                     /translation="MPRNIPGKIIAIDDPAASGKNTIAPIQRKFTRSCLLPILVFLGT
                     LLLAACAPMHGGRGGYLNVIPVDQRPPLVGAGALADSLYGPAGESERGDGIAESRRAE
                     LEALVRRFAPTLILPDGDQATVNGRTYQLLPSDPQLFAETLQIDLICAAPYHFCDSLD
                     ILFRNLSTDSLAKLVEAAGRYESDPQVFAVWYFDFHGGNPREWWRTYGRLRTGPDSLL
                     WAQPTVYAHPFLDFLGRAVIQYWYFYAFNDFVGNHEGDWEHVNVVLTRGRDSVAEVHY
                     FFHARSMKLPQGAYRPRIVDQTHPVVYAGGRAYRLFDYSIQLFTGDLNAGSHGNYPYS
                     GRWEGAIALSQTEIVSPLGEDSSRVVPHDQFRVVLTPEPSHIDFRRKPEILKEWAWLM
                     LPVRWGFPSAPSVGAELKITDVGNRAPFGPAFNAGWNRTVHGFLYSPYAVRRIGVVRT
                     TINDLLQPWNFLYLYRYPRYFRTARYVHDTQSA"
     gene            complement(1747..2397)
                     /gene="scpB"
                     /locus_tag="AC16GM_06635"
     CDS             complement(1747..2397)
                     /gene="scpB"
                     /locus_tag="AC16GM_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR2923387.1"
                     /translation="MEPKPTQIIEALLFAADHPLTAEEIAAALPEEASIPDLIDDLNR
                     FYETSGRAFRIREVAGGYQLATRPEFAPHVNSLFAGTRAQKLSRAGLETLSIVAYKQP
                     VIKAEIERIRGVQSDGVLKTLLERDLVAICGRQEGPGRPLLYGTTDKFLEYFGLKDLS
                     GLPTPDELGTLLSRQKLTELPATSPELELTLTSDASATNGDPVAADNAHAPESDTS"
     gene            complement(2369..3298)
                     /locus_tag="AC16GM_06640"
     CDS             complement(2369..3298)
                     /locus_tag="AC16GM_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR2923388.1"
                     /translation="MEPTLTYKKQDDSAPLLVKLDLFEGPLDLLLFLVQREEVPITDI
                     PIAKITQQYLDYLKAMETFNLEVAGEYIVMAATLIYIKTQLLLPRPQLEGEEGDPRAQ
                     LVQALVEYRRYKEAAVSLEKREETERQIFPHRDTSFLALKPEAPFELTATLFDLALAF
                     KEACERLPESALGGLEITYRRVTIEERIAHVLSVLKAKKRVPFWELYADEPVRPILIV
                     TFLALLELVRLQKLHLLQKDRTGEIWVMRFRAGERSDAVKEFPSEASESSAPVDRIKD
                     KRLISPVRKEPKRPVRKLIIKRGSDGAEAHPDH"
     gene            complement(3298..4299)
                     /gene="trpS"
                     /locus_tag="AC16GM_06645"
     CDS             complement(3298..4299)
                     /gene="trpS"
                     /locus_tag="AC16GM_06645"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR2923389.1"
                     /translation="MSPARSGAGQSRPVVLSGMQPTGRLHLGNLEGALANWVKLQDEY
                     ECYFCIVDWHALTSDYDQTKDLKERIFQMTVDYLAAGLDPDKCAIFVQSQVKEHAELH
                     LLLSMITPVPWLQRVPSYKEKSEELKLDSYGFLGYPLLQAADILLYRANHVPVGQDQL
                     PHIELTREVVRRFNHLYGNVFPEPEALLTKFPVIPGIDGRKMSKSYNNDIAIADSPEI
                     TAKKISRMYTDPRKIYLGDPGHPEECPVYALHQIYNPQHETEIAPHCRSGVLACVDCK
                     KRMANYPNTALAPICERRLKLEKDKAAVMDVLKNGAEKARAKAQKTMEKVRKAIGLL"
     gene            complement(4296..5075)
                     /locus_tag="AC16GM_06650"
     CDS             complement(4296..5075)
                     /locus_tag="AC16GM_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923390.1"
                     /translation="MREGSSFARKRRLVTRWTATVAGLIFFTICKASHAQFPVIQGDF
                     DISPLKTLRINYVLKGKLSGDQTLYIGGNRALVEIEAGTAAPDNQKLSLKEYYSPEAK
                     IVYDAARQVGRKADNVAYYRKQQIALLSKADRRYYEKHRGQAQSSELSRLLGGTGAEK
                     VSFLGKQCDVYRRPNLEVYLWKNIVVKMKVPALKIDMEATGIYENPKIPEDFLALPVG
                     MPIAYDEKYSRAAKAGVDSLILAIKNRKLDQTPPRPRSGSK"
     gene            complement(5298..5822)
                     /locus_tag="AC16GM_06655"
     CDS             complement(5298..5822)
                     /locus_tag="AC16GM_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR2923391.1"
                     /translation="MNADGGILRGNVLVLNGNYEPLSVCSVKRAVVMVFLGKAEVVER
                     HDGDSVHSVSVAIPVPSVVRLGFYIAVPQKRILLTRKNVIKRDGHRCQYCGEVRGPMT
                     VDHVIPKMYGGKDVWENLICACLRCNNIKGDRTPDQAGLSLLRQPKRPNHVTFIQQFI
                     GVSDLRWRPYLFLD"
     gene            6154..7920
                     /locus_tag="AC16GM_06660"
     CDS             6154..7920
                     /locus_tag="AC16GM_06660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR2923392.1"
                     /translation="MNFDRRSQIVALILAIVVLGIVWVTGPGQAQSGWTLAQTDTLKA
                     PEVIESSPETLYRNVKIFDDIAFNVNARYMDDVNPKELITAGIRGMMETLDPFSVLME
                     RKPYERLMEATHGKYEGLGMQIEQRENRIIIVTPIEGTPAYRMGFQAGDVIMEIDGKS
                     TERMTTEDASRLMRGTAGTTVNLKIMREGFKAPVDYTVERAVITLKSVNYYGVTQNNI
                     GYLRLSRFSEDTHQELHLALADLASRKINGLIFDLRNNGGGLLDEAVSTANLFLGRDK
                     LIVYTKGRDPDEEKRYTAPEDAFFKDGPLVVLVDEQTASASEIVAGAIQDWDRGVIIG
                     DTTFGKGLVQQVFPVSGSRDLFLKLTTARWYVPSGRTIQKPERSTKHPELLGQEGDEE
                     DTTQSKPQKPDKTEVYRTKAGRLVYGGGGIIPDIIMDRERWRPIEWNLERQRLFFQFA
                     VVYTTKHPDLPQNFGVDEAMLAEFKSFIKEKKFDYKTPLEVELDSLASLAAQDNKQEV
                     FADAVTLLRQKITAEKEKDYDASRDYIAKAIKREILLKKFGEKGYYEELIFKDDPYVT
                     RALDVLSNKSQYTRLLRPLAER"
     gene            complement(8288..9199)
                     /locus_tag="AC16GM_06665"
     CDS             complement(8288..9199)
                     /locus_tag="AC16GM_06665"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR2923393.1"
                     /translation="MAKKALVLSGGGAKGAFQFAAEKYAREVKSYRWDVIAGVSVGAL
                     NGVMLAMQKYQRLEEIWQTISNDKVYTGGLNVWSVAKVLLFGARSFYGNKPLWKMIDE
                     NVELDKIKIDLTIGAVSLQTGKYVAFKPRDTKNSGFSNELEFKKAVLASTVMPIIWPP
                     VEMSPSLADMVDGGLRNISPLGDVLDANPDEVIIINCNPRDLDPPELGRPLKNLPDIG
                     KRSLEIVLNEIFNNDIDEFIRINKNVEEARAKELTLHNEDGKPYKYFKYKIIEPDHHL
                     DDTIDFSQGACGRSMDAGLKKATEVLG"
     gene            complement(9358..>9662)
                     /locus_tag="AC16GM_06670"
     CDS             complement(9358..>9662)
                     /locus_tag="AC16GM_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923394.1"
                     /translation="GNEPKRASAAPPPQYEPQSTQPPDAGWSGTDSYKQNTKEKERGK
                     SDEPKKGQGEEARRTEKRKAYDSFIDRNGNGIDDRYEKKTRGTEESGSKKKKKPGE"
BASE COUNT         1965 a         2797 c         2768 g         2132 t
ORIGIN      
        1 cgcgctctgg gtgtcgtgga cgtaccgggc cgtgcgaaag tagcggggat agcggtagag
       61 gtagagaaaa ttccagggtt gcaggagatc gttaatggtg gtccgcacca cgccgattcg
      121 cctcaccgcg tacggcgagt agagaaaccc gtgcacggtg cggttccagc ccgcattgaa
      181 agccgggccg aagggcgccc ggttgcccac atcggtgatt ttgagctcgg caccgacgga
      241 gggtgcgctg gggaaccccc agcggacggg gagcatgagc caggcccatt ctttgagaat
      301 ttccggtttg cgccggaagt cgatgtggga gggttccggc gtcaggacga cgcgaaattg
      361 gtcgtgcggc accacccggc tggagtcctc gcccaaaggc gaaacgatct cggtctgact
      421 caaggcgatg gcaccttccc acctcccgga gtagggatag ttgccatgtg acccggcatt
      481 caggtcgccc gtgaagagct ggatggagta gtcgaagagc cggtaggccc tcccgccggc
      541 gtaaaccacc ggatgcgtct ggtcgacgat ccggggtcgg tacgctccct gcggcagttt
      601 catgctccgg gcgtgaaaaa agtaatgcac ttccgcaaca ctatcgcggc cccgggtgag
      661 cactacgttg acatgctccc aatccccttc gtggttgccg acgaaatcgt tgaaggcata
      721 aaagtaccag tactggatta ccgctcgtcc gaggaagtcg aggaagggat gggcgtagac
      781 ggtgggctgc gcccagagga gactgtccgg accggtgcgc agccggccgt aggtccgcca
      841 ccactcgcgg ggattcccgc cgtgaaaatc gaagtaccag accgcaaaaa cctggggatc
      901 gctctcatag cggcccgccg cctccaccag cttggccagc gagtcggtgg atagattccg
      961 gaagagaatg tctaaggaat cacagaaatg gtacggtgcg gcgcaaatca agtctatttg
     1021 caaggtttcg gcgaaaagct gcggatcgct gggtaaaagc tggtacgtcc gcccgttgac
     1081 ggttgcctga tccccgtcgg gcagaatgag ggtgggcgcg aaccgccgca cgagcgcctc
     1141 cagttcggct cgccgagact cggcgattcc gtccccgcgt tcgctctccc cggccggtcc
     1201 atagagcgaa tcggccagcg cacccgcacc cacgagcggc ggccgttgat cgaccggaat
     1261 gacattcagg tagcccccgc gcccgccgtg catcggagca cacgcggcta agagcaaagt
     1321 tcccagaaaa accaatatcg gcagcaagca cgaacgggtg aactttctct ggatgggagc
     1381 tatggtgttt ttgccggagg ccgccgggtc gtcgatggcg atgatttttc cgggaatatt
     1441 tctgggcatc agatcaaaaa gataagtgga gatacgctat cggccaacgg cgcaaaccac
     1501 aattagcgac cagatttcaa atttccgtta cgggtgctgg aacctataac cggctcaggg
     1561 acgagccggt gtaccttgga tcggtagtcg ggctcgtctc tgagcccgac ttcaaaacca
     1621 aaaggtgggg ctgaagcccc gcctacgcgg taaggtataa cagcagacct agcttttcgc
     1681 gttggcagca ctgaagtgcc gccgattgga tggggtttcg gacgtaatcg gcgcttgaaa
     1741 ctgcagctat gaggtatctg actcgggcgc atgcgcgttg tctgccgcca ccggatcgcc
     1801 gttggtggcg gaggcatcag aggtcagcgt gagttccagt tccggagacg tggccggcag
     1861 ctcggttaat ttttggcgag acaaaagcgt gccgagttcg tccggggtag gtagaccgga
     1921 gagatccttc aagccgaagt attcgaggaa tttgtcggtg gtgccgtaga ggagcggccg
     1981 gcccgggcct tcctgccggc cacagatggc caccagatcg cgttccaaaa gcgtcttcag
     2041 cacgccgtcg gactggacgc cgcggatccg ctcgatttcg gccttgatta ccggctgctt
     2101 gtaggcgaca atcgacagcg tctccaaccc ggctcgggag agtttctgcg cccgcgtccc
     2161 ggcgaaaaga gaattgacgt ggggagcaaa ctccgggcgc gtggccaact ggtaaccgcc
     2221 ggccacctcc cggatgcgga acgcccggcc gctcgtctcg tagaaacggt tcaaatcgtc
     2281 aatcaaatcg gggatcgacg cttcttcagg cagtgccgcg gctatctcct ctgcggtgag
     2341 cggatggtcg gcggcgaaga gcagcgcttc aatgatctgg gtgggcttcg gctccatccg
     2401 atcctctttt gatgatgagc ttccgcaccg gacgcttcgg ctctttgcgc acgggagaaa
     2461 tcaaccgctt gtcttttatc ctgtcaactg gagcggaaga ctcggaagct tcggacggga
     2521 attcttttac tgcatctgaa cgctctccgg ctcggaagcg catcacccag atttctcccg
     2581 tgcggtcttt ctgcaaaagg tggagctttt gtaaccgcac caattccaga agcgcgagga
     2641 acgtgacaat caggatcgga cgcaccggtt cgtccgcata cagctcccag aatggcaccc
     2701 gttttttggc cttcaagacg gaaaggacat gggcaatgcg ctcctcgatg gtgacgcgcc
     2761 ggtaggtaat ttccagtccg cccaaggccg attccggcag cctttcacag gcttccttga
     2821 aagccaacgc taaatcaaag agcgtggcgg tcaactcaaa aggggcttcc ggtttgagtg
     2881 cgaggaaact cgtgtcgcgg tgcggaaaaa tctgacgttc ggtttcttcg cgcttctcca
     2941 gtgaaactgc cgcctctttg tagcgtcgat actctacgag cgcctggacg agctgggcgc
     3001 gggggtcacc ttcctcgcct tccaactgcg gccgcggcag gagcagctgg gtcttgatat
     3061 agattaaggt cgccgccatc acgatatatt ccccggcgac ttccagattg aaggtttcca
     3121 tcgccttgag gtagtccagg tactgctggg tgattttcgc gatcgggatg tcggtgatgg
     3181 ggacttcctc ccgctggact aaaaagagca agaggtccag cggcccttcg aagagatcga
     3241 gttttaccag aagcggcgct gagtcgtctt gctttttgta agtaagtgtc ggttccatta
     3301 caaaagccct atcgctttgc gcactttttc cattgtcttc tgggctttgg cccgggcttt
     3361 ttcggctcca tttttgagca cgtccatcac ggcggcctta tccttttcca gcttcagccg
     3421 ccgttcgcaa attggcgcca acgccgtatt ggggtaattt gccatccgct tcttacaatc
     3481 aacgcaggcc agaactccgg agcggcagtg cggcgcaatt tcggtttcgt gctggggatt
     3541 gtaaatctgg tggagcgcgt agaccgggca ctcctccggg tggccggggt cgcctaaata
     3601 aatcttgcgc ggatcggtgt acatccgcga aatctttttg gcggttatct ccggcgaatc
     3661 ggcgatggcg atgtcgttgt tgtacgattt cgacatcttc cgtccgtcga ttcccgggat
     3721 caccggaaac ttcgtcagga gcgcctccgg ttcggggaaa acgttcccgt agaggtgatt
     3781 gaagcgccgc accacctcgc gggtcagttc gatgtgcggg agttggtcct gacccaccgg
     3841 gacgtggttg gcacggtaga ggagaatatc cgccgcttgc aggagcgggt agcccagaaa
     3901 gccgtaactg tcaagtttga gttcttctga tttttctttg taggacggca cccgctggag
     3961 ccagggaacc ggcgtgatca tcgagaggag cagatgcagt tcggcgtgct ctttgacctg
     4021 cgactggacg aaaatcgcgc acttgtccgg atcgagcccc gccgccaggt aatcgaccgt
     4081 catctggaag atacgctctt tcaaatcttt ggtctggtcg tagtccgaag tgagcgcatg
     4141 ccaatcgaca atgcaaaagt agcactcgta ctcatcctgc agtttcaccc agttcgccag
     4201 cgccccttcg aggttgccca ggtgcaaccg cccggtgggc tgcatgccgg agagtacgac
     4261 gggtcgggat tgacccgcac cggaccgggc cggactcact tggatcccga cctcggcctg
     4321 ggtggcgtct ggtcgagttt tcggttcttg atggctaaga tgagcgaatc gacgcccgct
     4381 ttggctgcgc gggagtattt ttcgtcgtag gcgatcggca tccccacggg cagcgccaaa
     4441 aaatcttccg ggattttcgg gttctcgtaa atgccggtgg cttccatgtc aatttttaat
     4501 gccgggactt tcatcttcac gacgatattt ttccacagat agacttctaa attgggtctc
     4561 cggtagacgt cgcactgttt gcccaggaaa gaaactttct ccgcgcccgt gccgcccaaa
     4621 agccgcgaga gttcggagga ctgcgcctgc ccgcggtgct tctcgtagta gcgccggtcg
     4681 gccttggaga gcagggcgat ctgctgctta cggtagtatg ccacgttatc cgccttgcgc
     4741 cccacctggc gcgcggcatc gtagacgatc ttcgcttccg gcgagtagta ttccttcagc
     4801 gacaacttct gattgtctgg cgcggcggtt ccggcttcga tttcgaccag ggcccggttg
     4861 ccgccgatgt agagcgtctg gtcgccggag agtttacctt tcaacacgta attgatgcgc
     4921 agcgttttga gcggggagat gtcgaaatcg ccctggatga cggggaactg ggcgtgggat
     4981 gccttgcaga tcgtaaaaaa aattagcccg gcgaccgttg ctgtccacct cgttacgagt
     5041 cttctttttc tggcaaaaga tgacccctcc ctcaccctcc cctccaaggg gagggaaaaa
     5101 ctttcatgca catttccttc aacaattgaa ggggttaaca ttccctctcc tcctagtggt
     5161 tcgaccttgc tcaccaccct gagcctgtcg aaggggagag ggttggggtg aggtcaaaag
     5221 aagaaaaccg caacgcgggt cgtgtgcctg gttccaactt ttccagccgg tgcctcctca
     5281 gttcgcgtcg tcctccgcta atctaaaaac agatacggcc gccagcgcag gtccgaaacg
     5341 ccgatgaact gctggatgaa agtgacgtgg ttgggccgct tgggctggcg caaaagcgaa
     5401 agccccgctt gatccggcgt ccggtcgcct ttgatgttgt tgcagcgcag gcaggcgcag
     5461 atcaaattct cccacacgtc tttgccgccg tacattttgg gaatcacgtg atccaccgtc
     5521 atcggcccgc gcacctcgcc gcagtactgg cagcggtgtc cgtcgcgctt gatgacgttc
     5581 ttgcgggtca aaaggatccg tttctgcggc accgcgatgt agaatcccag gcgcacgacg
     5641 gaaggaacgg ggatcgccac ggaaaccgaa tgcaccgagt cgccgtcgtg ccgctccacc
     5701 acctcggctt tgcccaaaaa gaccatcacc accgcccgct tgaccgagca gaccgagagc
     5761 ggctcgtagt tcccgttcaa aacgagcacg ttgccgcgta atatgccacc gtccgcgttc
     5821 atcgccaatc agttcgctct tggtaattta tacgcctctc tccgtctgtc aattgcctcc
     5881 gcaacagttt cggtttcttg taattcaccg cctgaaggga aactcaatct tttccgggaa
     5941 cgaaaccctc ctcccctgcg tatcccatat gaaagaaaac tgttgacttt ttgcgtaaat
     6001 gccttattat aaaactgccc gctcggtacg agtctattgc gaccggaagc ggaattgaca
     6061 ggtgatagga ccccgaaaaa ggataaattc cataggacgg tccgcctcag gcagatcgat
     6121 tttggacgtt tctgaaagga gattggagac gctatgaact tcgaccgccg ttcccaaata
     6181 gtcgcgctga ttctggccat cgttgtcttg ggcattgtct gggtcaccgg gccgggccag
     6241 gcccagtccg ggtggacttt ggcgcagacg gacaccctca aagctccgga agtcatcgaa
     6301 tcctctccgg aaacgctcta ccggaacgtc aagatcttcg acgacatcgc cttcaacgtc
     6361 aacgcccgct atatggatga cgtcaacccc aaggagctca tcaccgccgg catccggggg
     6421 atgatggaaa cgctcgaccc cttctcggtc ttgatggagc gcaagcccta cgagcgcctg
     6481 atggaggcca cccacggcaa gtacgagggg ctgggaatgc agatcgaaca gcgcgaaaac
     6541 cgcatcatca tcgtcacgcc gattgaaggc acccccgcct accggatggg cttccaagcc
     6601 ggcgacgtca ttatggaaat cgacggcaaa tcgaccgaaa ggatgaccac cgaagacgcc
     6661 tcccgcctga tgcgcggcac cgccgggacg actgtgaact taaagattat gcgcgaaggg
     6721 ttcaaagcgc cggtggacta caccgtggag cgggcggtga tcaccttgaa gagcgtcaac
     6781 tactacggcg tcacccagaa caatatcggc tacctgcggc tctcccgctt ttccgaagac
     6841 acccaccagg aactgcacct ggccttagcc gacctggcca gccgcaagat caacggcttg
     6901 attttcgatc tgcgcaacaa cggcggcgga cttctggacg aagcggtttc gactgccaat
     6961 ctctttttgg gccgggacaa attgatcgtt tacaccaaag ggcgcgaccc ggacgaggaa
     7021 aagcgctaca ccgcaccgga ggatgctttc ttcaaagacg gcccactggt cgttttggtc
     7081 gatgagcaga ccgccagcgc ctcggagatc gtggccggtg ccattcagga ctgggaccgg
     7141 ggcgtcatta tcggcgatac gaccttcggc aaggggctgg tgcagcaggt ttttccggtg
     7201 agcggctccc gcgacctttt tttgaaactg accaccgccc gctggtacgt cccctcgggc
     7261 cgcacgattc aaaaaccgga gcggagcacc aagcatccgg aacttttggg acaggagggt
     7321 gacgaggaag acaccaccca gtccaaaccc cagaaacccg acaagaccga agtctaccgc
     7381 accaaggcgg gacgactggt ctacggcggc ggcggcatta tccccgacat cattatggac
     7441 cgcgaacgct ggcggccgat tgagtggaac ctggaacggc aaaggctctt cttccagttc
     7501 gcggtggttt acactacgaa acaccccgac ctgcctcaga atttcggagt cgatgaggcc
     7561 atgttagccg agttcaaatc gttcatcaaa gagaaaaagt tcgactacaa aacgccttta
     7621 gaagtcgaac tcgacagtct ggcttcgctg gccgctcagg ataacaagca ggaggtcttc
     7681 gccgatgcgg tcaccttgct ccgccagaag atcaccgccg aaaaagaaaa agactacgac
     7741 gccagccgcg actacattgc caaagcgatt aaacgtgaaa tccttttgaa gaagttcggc
     7801 gagaaggggt attacgagga attgatcttc aaagacgacc cttacgtcac ccgggcgctg
     7861 gatgtcttgt ccaacaagag tcagtacacc cgactcctgc ggcctttagc ggagcggtaa
     7921 tcttcagtct actccctctc ctttaggaga gggttggggg agaggtcagc ccttttgggt
     7981 cattcttgac cccaccctac cctcccctac aggggagggt aaaagtcatc cgtcctttcc
     8041 tgggaaactg agatttatgc tccctctcct gcaggagagg gccggggtga ggtcagcctt
     8101 ttgggtcatt cttgacccca ccttaccctc ccctacaggg gagggtaaaa tttatccgta
     8161 ctttcctggg aatctgagat ttatgctccc tctcctttaa ggagaaggtt ggggagaggt
     8221 caaccctgtg ggacattttg acccctccct cgccctcccc ttaaggggag ggaagttcca
     8281 ttctctttta ccccaggacc tctgtcgctt ttttcagccc cgcatccata gaccttccgc
     8341 aggccccctg ggaaaagtcg atagtatcat ccaggtggtg gtcgggctcg atgattttat
     8401 acttgaagta tttgtacggt ttcccatcct cgttgtgcaa cgttaactcc ttcgccctgg
     8461 cctcttcaac attcttgttg atccgaatga attcgtcaat atcgttgttg aaaatttcgt
     8521 tgagcactat ttccagcgag cgcttgccga tgtccgggag gttcttgagc ggccggccca
     8581 actccggcgg gtccaggtcc cggggattgc agttgataat aatgacttca tcgggattgg
     8641 catccagaac gtcgcccaag gggctgatgt tcctcagccc cccgtccacc atgtcggcga
     8701 gcgatgggga catttccacc ggcggccaga tgatgggcat aaccgttgag gccaggaccg
     8761 ctttcttgaa ctcaagttca ttgctgaagc cggagttttt ggtgtcccgg ggcttaaacg
     8821 ccacatattt ccccgtttgg agagagaccg ctccgatcgt caggtcgatt ttgattttgt
     8881 cgagttcaac attctcgtcg atcattttcc agagcggttt attgccgtaa aaggacctcg
     8941 ccccgaagag cagcaccttt gcgaccgacc agacgttcaa tcccccggta taaactttat
     9001 cgttggaaat cgtctgccat atttcttcga gacgctggta tttctgcatc gccagcatca
     9061 cgccgttgag cgcacccacc gagactcccg caatgacgtc ccaccggtaa ctcttcacct
     9121 cgcgggcgta cttttccgcc gcaaattgga acgcgccctt ggcacctccg ccgcttaaaa
     9181 cgagagcctt cttcgccatg cttcctccca atgagttgat aagttatccg ctaatgaatc
     9241 agggccaaac ttccgtctgg ccctgatcct gtttcagcca cctctgtcgg ggtcggggac
     9301 gaccccgact acccctcggt ggttcggttc atctccgaaa ccgaatacga atgcgactca
     9361 ctctcccggt ttctttttct tcttgctgcc ggactcttcg gtgccccggg ttttcttctc
     9421 gtagcggtcg tcgatgccgt tgccgttgcg gtcgatgaag ctgtcgtacg ccttgcgctt
     9481 ctcggttctg cgggcttctt cgccctgccc tttcttcggc tcgtcagatt tccctcgctc
     9541 tttctctttc gtattttgtt tgtacgaatc cgtaccggac catcccgcat ccggcggttg
     9601 cgtcgattgc ggctcgtact gcggaggcgg cgctgcgctg gcgcgtttgg gttcgttgcc
     9661 gg
//
LOCUS       DBNGZD010000253         9250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_40069, whole genome shotgun sequence.
ACCESSION   DBNGZD010000253 DBNGZD010000000
VERSION     DBNGZD010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_40069"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1584
                     /locus_tag="AC16GM_06675"
     CDS             151..1584
                     /locus_tag="AC16GM_06675"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR2923395.1"
                     /translation="MRELAAALAAETRALPGWNIFSSRAFLLVFGAAALSVWIAAGAG
                     PVAALPILALVVAVGLALADLKKLVLFFLVSRSALDGIRQYDFTLGALSLNASGFMGL
                     CLIGLALLVIWQKQINPWRDRFGRIFCLFLTVSGFGVGVAVWHFGGEGIVAVKEWIRL
                     ASLFSLFFLLTTLFTTPADLKNLWRAAFWSLPVPLVVGLIQAVTGTGDTISTEGQNRI
                     YGTVVHPNSLGSFLVLFMVMAWIWRSQSKRKWPLTTLLGILALTLFLTYSMTGLAHLA
                     VSFLVIGLATNRRATVVLLLLGALALGFSANWQARWEQVKEMNVAEEVSSTDITNSFS
                     WRVLHWVVLCNLWKDEPLFGYGLFMTEKVTPWKTIDGQGFAAHNDFVRLLLETGIVGT
                     LVYLFFLLGLGKFLLSLYRRARAPAARRLVFGVGAVYIAILAESFGAGEPLMHTAFLY
                     YFFTFLAIAAVWIRQENQSNSEHFSAV"
     gene            1674..2657
                     /locus_tag="AC16GM_06680"
     CDS             1674..2657
                     /locus_tag="AC16GM_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2923396.1"
                     /translation="MDNIRYLVTGGAGFIGANLVAELVRRGERVRVLDNFSTGRPENL
                     QNLIHRIELWAGDIGNLAFVHRAVVGCDFILHLAARPSVARSVENPLATNDTNVTGTL
                     NLLWAAREAKIRRFVYASSSSVYGEGPPLPTEESQEPRPLSPYAVSKLAAEQYTRVFW
                     ELYRVPTVCLRYFNVYGPLQSPGSAYAAVIPRFIHASLHDLRPQVFGDGLQSRDFTFV
                     SDVVRANLLACAEDQAVGQTLNIACGQSHSLNELLNQLERISGKKVAPIYLDPRPGDI
                     RHSQAAIKKAQGILGFTAETSFAEGLEKTYRWFAEKEGRLRPREEHALSFA"
     gene            2638..3867
                     /locus_tag="AC16GM_06685"
     CDS             2638..3867
                     /locus_tag="AC16GM_06685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2923397.1"
                     /translation="MPSVLRKRKPVSSQAGAVPGSPLAVTFVYPTDPRGVKVGGVETF
                     IRSFLELAPTEVEARLVGIDTAGDLACGTWQTVTVSDRPVLFFPAFFEKHQANRRHPV
                     PLSLRFALQLWCFRRRLAVDNSVLSVHRVELSWPFWGHPRLVLFIHGASKFLSLPSES
                     RLRWIRPFFASVEARALEKARRAVVVSQEGLDYYRNRFPGLNGKMRLISNWVDTQTFY
                     PRNKAALRRKFQIPLHVPVVAFVGRFEKVKDPELLLAVFARIKAEFPKALLVVCGAGE
                     EVRGQIPRELQVAVRLYGAVPHQALAEILGCADLFLLTSFFEGMSRAVLESLACGVPV
                     VATDVGDIRKVVSNNLSGSVANSRSPEELAARGVAVLQNESITAKSCVAAIRGFTAPE
                     TIGRIVALHREVANGQE"
     gene            3854..4618
                     /locus_tag="AC16GM_06690"
     CDS             3854..4618
                     /locus_tag="AC16GM_06690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2923398.1"
                     /translation="MGRSETTPAGVAAKTLVREFQKGYYTRQYGNRLFFSGSDKPVWF
                     GAWVRLLRKYLPEQAHLLEVGCGEGHFLRRAQKVFGRVSGLDISAEGVAQARKAARGV
                     EVRLADASHLPFPAGAFDAIVAFDLIEHLSEPNKFLAEAFRVLRTGGLLVLSTPNPDS
                     FGHRFKKERWFGYRDSTHVSIQSPHEWFLRLKQAGFLPLKTGTDAWWDAPYLPFVPHK
                     LQKIFFAGLANASFLISPIWPWQSGENFVGIFRKNG"
     gene            4620..5753
                     /locus_tag="AC16GM_06695"
     CDS             4620..5753
                     /locus_tag="AC16GM_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2923399.1"
                     /translation="MRIAFDATSLARPQTGIETYARHLLAALLREKSPHAWVVLLRGG
                     MPEELRELAQRGRWLNCPANSQLFCEQVWIPRVLAKERVDLAHFPAFPPSPLVSVPFI
                     YTAFDATLWRNWPLLSWKGKFYFRPLADLALRRARKVLTISESSRRDLQSFFPGLNGT
                     VANAGIGISEDFFTSRRLTRSDAEAVRRRYRLPQRFILSVGSLEPRKNLKTLLWAFAR
                     YGELHPADKLSLVLAGRPAWGAAEVAGLQKHPALRNRLVFTGYVPQVDLPKIYRLATM
                     LVFPSRYEGFGLPVAEAFASGTPVVASQIPAVVEQADGAALLVDPFDVEGFAQAMARL
                     SADGKLRRRFAAQGRAAAARYRWQKVAEQVLAIYAEVGQCRKK"
     gene            5738..6493
                     /locus_tag="AC16GM_06700"
     CDS             5738..6493
                     /locus_tag="AC16GM_06700"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR2923400.1"
                     /translation="MPEKVTLLDLTIDNVDLGEAIGRIEQLVAGRRGAFVVTPNVDYM
                     VNLRRDQKFRQIVNEAQLVLADGMPLVWASRFLGTPLKERVTGADLFPKLLERAAKHH
                     WKVFLLGGKPGVAQRIARRVRERFSGLPLVGCYSPPFGFENDVYALGEIAALVGAARP
                     DLLCVGLSSPKQEQLIVYLRQKEKIPLIVGVGAAFDFAAGTLRRAPVWMQRVGLEWAF
                     RLASEPRRLWRRYLRDTMFFWYVLQQKLSSRTA"
     gene            complement(6610..8910)
                     /locus_tag="AC16GM_06705"
     CDS             complement(6610..8910)
                     /locus_tag="AC16GM_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923401.1"
                     /translation="MTLMTLIVTLAVSAFLVVNAGAAVPQLINYQGKLTDNSGAPITS
                     AVTLKFRIYDAPAGGTLLWGPESHPVTPDANGDYNVILGAGTPPVAFPANLFAATPRY
                     LGVEVNTDGEMTPRQQITAVAYSLRVATVDGATGGTISGNTAIQSDLTVSGDVGIGTA
                     SPAQKLDVAGTAQMTGFKLPTGASNGFVLTSNAVGVGTWLAAGAGSGWSLTGNSGTTP
                     GTNFLGTTDNQALELKVNGSRALRIEPNTTSPNLIGGQSGNSVTSGVRGATIGGGGTI
                     AFTNNQVTDDFGTIGGGDANQAGDNAGTTSDRTHATVGGGFSNTASGDDATVGGGNTN
                     TASGGGATVGGGILNTASGSRATIGGGFSNIASGRFATVGGGLSNTASGDFSFAAGRR
                     AKADHLCTFVWADSSADADFASTADNQFLIRAVNVGIGTNNPANKLDVEGGAVIGATY
                     SGTNLAPTNGLLVEGNVGIGTTGVSAQLDVEQLGATVANFNRSSTDGTLLQFQRDAVT
                     VGSISVAAGVVSYNAFTGSHYAWTDEVLGRGELVTLTGINGNSHNDPESEVIYGIKRS
                     TVANDPACLGSYLALQELSQPASTENPHLVMAVGNGDMWVTDESGDIHPGDYLISSST
                     PGHAMKDDEAKYPLGYVIARAADPVNWGNESEMVGGHKHKRISVLFESFVRNSTTDLV
                     KLVETQQETLTKQQKEIDELKALVKTLVAKEQPDGKSIGQASSPARGGTSSLTETVRQ
                     LNIYFGMSPYGSIGIAPTLPLLRGKL"
BASE COUNT         1675 a         2752 c         2746 g         2077 t
ORIGIN      
        1 gcccggaagc gattgccagc ggtatgattc aggttttggg caaccgcagc ctggcttccc
       61 ggctctccct tcagggctcg cggcgcgccc gccagttttc ccaccgggcg atggcgcgga
      121 aatttatgac ggtctgcgag gaactgctcc gtgcgtgagt tggcagccgc cctggcggcg
      181 gagacaagag cgctcccggg ttggaatatc ttttcctccc gcgcattcct tttggtcttc
      241 ggcgcggcgg ctctaagcgt ctggattgcg gccggagccg gaccggtggc ggcacttccg
      301 atcctggcac ttgtggtcgc cgtcggtctg gcgctggccg accttaaaaa gctcgtgctc
      361 ttctttctgg tcagccgctc ggctttagac ggcatccgtc aatacgattt taccctggga
      421 gctctttcgc tcaatgcttc cggcttcatg gggctctgcc tgatcggcct ggcacttttg
      481 gtcatctggc aaaagcagat caacccatgg cgcgatcggt ttggacgcat tttctgtctt
      541 ttcctcaccg tctccgggtt cggggtgggc gtggcggttt ggcatttcgg cggtgagggg
      601 atcgtcgccg tcaaagaatg gatccggctg gccagtctct tttcactttt ctttttgctc
      661 accacgctct tcaccactcc ggcggatctc aaaaatctct ggcgcgccgc tttctggtcg
      721 cttcccgtgc ctctggttgt gggcttgatc caggcggtca ccgggaccgg tgacacgatc
      781 tccaccgaag ggcagaaccg catctacggc accgtcgttc acccgaacag cctgggatcg
      841 tttttggtcc ttttcatggt catggcctgg atctggcgta gtcagtccaa gcgcaaatgg
      901 cctttgacca cccttttggg cattctcgct ttaaccctgt ttctcactta cagcatgacc
      961 gggctggcgc acctggcggt gagttttttg gtcattggtc tcgcgaccaa tcgccgcgcg
     1021 accgtcgtgc tccttttgct cggagccctg gccctcgggt tttccgccaa ctggcaggcg
     1081 cgctgggagc aggtgaaaga gatgaacgtc gcggaagaag tttcctccac cgacatcacc
     1141 aattccttct cctggcgcgt gctccactgg gtggtgctct gcaacctttg gaaggacgag
     1201 cccctcttcg gctacggcct gttcatgacc gaaaaagtga cgccctggaa gaccatcgac
     1261 ggacagggat ttgccgccca caacgatttc gtgcggctcc ttctggagac cgggatcgtc
     1321 ggcacgctgg tctacctttt ctttttgctc gggctgggga agttcttgct ctccctctac
     1381 cgcagggcgc gcgccccggc cgcgcggcgt ttggtgttcg gcgtcggcgc cgtctacatc
     1441 gccattctcg ctgaaagctt cggggccggc gagcctttga tgcacaccgc gttcctgtac
     1501 tactttttta cctttctggc catcgccgca gtctggatca ggcaggaaaa tcaatcaaac
     1561 tcagagcatt tctctgcagt ctgagagagg agctcatttg tttcagcgcg gggagagcat
     1621 aacgccgtga tcacaggagc cgataagaaa gatagcggag gccttgccca aggatggaca
     1681 acatacgcta cctggtgacc gggggcgccg gtttcatcgg cgccaacctc gtggccgaac
     1741 tggtgcggcg cggggaacgg gtccgcgttt tggataactt ctccaccgga cggccggaga
     1801 atctccagaa cctgatccac cggattgagc tctgggccgg ggatattggt aatctcgctt
     1861 tcgttcaccg ggcggtggta gggtgcgatt tcattctgca cctggcggcc cggccctccg
     1921 tggcgcgctc ggtggaaaac cctttggcaa ccaatgacac gaacgtcacc ggtacgctga
     1981 acctcctctg ggcggcgcgg gaagcgaaga ttcggcgatt cgtttacgcc tcttcctcct
     2041 cagtctacgg cgagggcccg ccattgccaa cggaagagtc tcaggaaccc cggcccctgt
     2101 ccccctatgc ggtgtccaaa ctcgctgcgg aacagtacac ccgcgttttc tgggagttgt
     2161 accgggtgcc taccgtctgc ctgcgttact tcaacgtcta cggcccgctt caatcccccg
     2221 gctcggccta tgcggcggtc atcccgcgtt tcatccacgc tagcctgcat gacctgcggc
     2281 cgcaggtttt tggcgacggc cttcaatcgc gcgacttcac tttcgtctcc gacgtggtgc
     2341 gggccaatct tttggcctgt gccgaagacc aagcggtcgg gcagacgctg aacattgcct
     2401 gcggacaaag ccattcgctg aatgaactcc tcaaccagct cgagcgcatc agcggaaaga
     2461 aggttgcacc catttacctc gatccgcgtc ccggcgacat ccggcactcg caggcggcga
     2521 tcaaaaaagc gcagggaatc ttggggttca ccgccgagac ttcgttcgct gaagggttgg
     2581 agaagactta ccgctggttt gcggagaaag aaggccgctt gaggccccgg gaggagcatg
     2641 ccctcagttt tgcgtaagcg aaaaccggtc tccagtcagg cgggcgcggt gcccggctcc
     2701 ccgctcgcgg tgaccttcgt ctaccccacc gatccgcggg gggtgaaggt cggaggcgtc
     2761 gaaacgttta tccgcagttt tctggagttg gcgccgactg aggttgaggc gcgtctggtc
     2821 gggattgata ccgcgggcga cttagcgtgt ggtacgtggc aaaccgtgac agttagtgac
     2881 cgcccggtcc tgttttttcc ggcattcttc gaaaagcacc aggccaaccg ccgccatccc
     2941 gtcccgttat ctctccggtt cgcactccaa ctctggtgtt tccgccgccg cctggctgtg
     3001 gacaattcgg ttctttcggt gcaccgggtg gaactctcct ggcccttctg gggacacccg
     3061 cgtctggttt tgttcattca tggtgcgtcg aaatttctat ccctgcccag cgaaagccgc
     3121 ctgcgctgga tccggccgtt cttcgcgtct gtggaagccc gggcgcttga aaaagcacgc
     3181 cgggcggtgg tcgtgagcca ggaagggctc gactactacc gaaaccggtt ccccggcctg
     3241 aacgggaaaa tgcgtttgat atcgaactgg gtggataccc aaacctttta cccccggaac
     3301 aaagccgccc tccggaggaa attccagatt cccctgcacg ttccggtcgt ggctttcgtg
     3361 ggccggtttg aaaaagtaaa agacccggag ttgcttttgg ccgtttttgc ccgcatcaaa
     3421 gcggaattcc cgaaagccct cctggtcgtt tgcggggccg gagaggaagt gcgcgggcag
     3481 attccccggg aactacaagt tgccgtgcgc ttgtacgggg cggttccgca ccaggccctg
     3541 gcggagatat taggctgcgc ggacttgttc cttttgacct cttttttcga aggaatgtcc
     3601 cgcgccgtcc tggagagtct ggcctgcggg gtgcccgtgg ttgccaccga cgtcggcgac
     3661 attcgcaaag tagtgtccaa taatctttcc ggctcggtgg cgaactcccg ctctccggag
     3721 gagttggccg cgagaggggt cgcggttttg cagaacgaat ctatcaccgc caaaagctgc
     3781 gtggccgcca tacgtggttt taccgcaccc gaaacgatcg gacgtatcgt ggcgctccac
     3841 cgggaggtgg ccaatgggca ggagtgaaac gacccctgcg ggtgtggcgg cgaagaccct
     3901 cgtacgagag tttcagaagg gttactacac ccgacagtac gggaaccggc tgtttttctc
     3961 cggttcggac aagccggtct ggttcggggc ctgggtccgc ctgttgcgca aatatctgcc
     4021 ggaacaggca catcttttgg aagtcggctg cggcgaaggg cacttcctgc gccgcgccca
     4081 gaaagtgttc ggtcgggtga gcggcctgga tatttccgcg gagggggtgg cgcaagcccg
     4141 gaaagcggcg cggggggtgg aagttcggtt agccgatgcc agccatttgc cttttcccgc
     4201 aggcgctttt gatgcgatcg tggccttcga tttgatcgag cacctttcgg aaccgaataa
     4261 atttttggcg gaggcgtttc gcgtgctgcg caccggcggt cttttggtgc tctccacgcc
     4321 caatcccgat tctttcggcc accggttcaa aaaagagcgc tggttcggct accgcgacag
     4381 cacgcacgtc tcgattcaat ccccgcacga gtggttcctc cgcctgaagc aggccgggtt
     4441 tttgcctctc aagaccggca ccgatgcctg gtgggatgcg ccgtacctgc ctttcgttcc
     4501 gcacaaactc cagaaaattt tctttgctgg cttggcgaat gcctcctttc tcatttcccc
     4561 catctggccc tggcagtcgg gcgaaaattt tgtcggtatc ttccggaaga acggatagga
     4621 tgcgcattgc cttcgacgcg acatctttag cccgcccgca aaccggtatc gagacctacg
     4681 cgcgccattt gctggcggcg ctcctccggg agaagtcgcc gcatgcctgg gtggtgttgc
     4741 tgcgcggagg gatgccggag gaattgcgtg aactggcaca gagagggcgc tggctcaact
     4801 gtccggccaa ctcacaactc ttctgcgaac aggtctggat tccccgtgtg ctggcgaagg
     4861 agagagtcga cctggcgcac ttccccgcct ttccgccgtc gcctttggtc tcggttccct
     4921 tcatctacac cgcttttgat gccaccctgt ggcgcaactg gccgcttttg tcgtggaaag
     4981 ggaagttcta cttccgtccg ttggccgacc tggcgctccg gcgcgcccga aaagttctga
     5041 cgatttccga gagttcccgg cgcgacttgc agagcttctt tcccggtctg aatggcacgg
     5101 tggccaacgc cgggatcggc atcagtgaag attttttcac cagccgaaga ctgacccgaa
     5161 gtgatgcgga agcagtccgc cgccggtacc gtctgccgca gcgcttcatt ctttcggtgg
     5221 ggtcgctcga accgcgcaag aatctcaaaa cactcctttg ggcttttgcg cggtacggtg
     5281 aacttcaccc tgcggataaa ctcagtctgg tcctggcggg gcgacccgcc tggggcgcgg
     5341 cggaggttgc cggactgcaa aaacacccgg cgctgcgaaa tcggctggtt tttaccggct
     5401 acgtcccgca ggtcgatctc cccaaaatct accgactggc gacgatgctg gtgttcccgt
     5461 cgcgctacga gggattcggt ctgccggtgg ccgaagcttt tgccagcggc acgccggtcg
     5521 tcgcctcgca aattccggcg gtggtggaac aagcggatgg ggcagctctt ctggttgatc
     5581 ctttcgacgt ggaaggattc gcacaagcga tggcgcggct ctcagccgac gggaaattgc
     5641 ggcgccggtt tgcggcccag ggacgggcgg ccgcggcgcg ctaccgctgg cagaaggtgg
     5701 ccgaacaggt gctggcaatc tacgctgagg tcgggcaatg ccggaaaaag taacgcttct
     5761 ggatttgacc atcgacaacg tcgatctcgg agaagccatt ggccgtatcg agcaactggt
     5821 ggcagggcgg cggggcgcct ttgtggtcac tcccaacgta gactatatgg tcaatctgcg
     5881 ccgcgatcaa aagttccgcc aaatcgtgaa cgaagcgcag ctcgtcctgg cggacgggat
     5941 gcctttagtc tgggccagcc ggtttttggg cacaccgctc aaagagcggg ttaccggcgc
     6001 cgatcttttc cccaaactct tggaacgggc ggccaaacat cactggaaag tcttcctttt
     6061 aggcggcaag ccgggcgtgg cgcagcggat agcgaggagg gtccgggagc gattctccgg
     6121 tctgcctttg gtcggctgct attcaccccc ttttggtttc gaaaacgatg tctacgcgtt
     6181 gggagaaatc gccgcgctgg tgggggcggc acgccccgac ctcctctgcg tcggattatc
     6241 ctctcccaag caggagcaac tcatcgtcta cctcaggcaa aaggagaaga ttcccctcat
     6301 cgttggtgta ggggcagcct tcgactttgc cgcgggcacg ttgcggcggg cgccggtttg
     6361 gatgcaaagg gttggcctgg aatgggcctt tcgcctggcc tccgaaccac gccgcttgtg
     6421 gcgccgttac ctgcgcgata cgatgttttt ctggtacgtc ctgcagcaga agttgtccag
     6481 ccgaacagcg tagaatcaag tcgaggaact gcgaagctcc gtgcaggttt cgaaaccaac
     6541 cccgccttgg gccccgccgt tggcgggatc tgcgacggga tcatcgacct gcactactgg
     6601 tttctgctcc tacaatttcc ctcgcaaaag aggtagagtc ggtgcgatcc ctatggatcc
     6661 gtatggactc atgccgaagt agatgtttag ttgtcttacg gtttcagtca ggctggaagt
     6721 cccgccacgt gcgggactgg aagcctgacc tatggacttt ccatcgggtt gttcctttgc
     6781 caccaacgtt tttaccagcg cttttaattc gtcgatttcc ttttgctgtt tggtcagagt
     6841 ctcttgctgg gtctcaacga gcttcacgag gtcggtggtg ctgttgcgca caaaactttc
     6901 gaacagaacc gaaatccgct tgtgtttgtg gccaccaacc atctcgctct cgttacccca
     6961 gttgaccggg tcggcagccc gggcaatcac atagcccagc gggtattttg cctcgtcgtc
     7021 tttcatagcg tggccgggag tagatgagga aatcaggtaa tcgccggggt gaatatctcc
     7081 gctttcgtct gtcacccaca tatccccatt gcctacggcc ataaccagat ggggattttc
     7141 cgtgctggcc ggttgagaca gctcttgaag cgcaaggtag gaacccaggc aggccgggtc
     7201 gttggcgacc gttgaccgct tgattccgta gataacctcc gattcggggt cattgtgaga
     7261 gttgccgttt atacccgtaa gggtaaccag ctccccccgc cccagaactt catccgtcca
     7321 ggcgtagtgc gagccggtga aggcgttgta gctgactaca ccggcggcga cggaaatgga
     7381 acctacggtt acagcatctc tttggaattg gaggagggtg ccgtcagtgg agctgcgatt
     7441 gaaattggca acggttgctc caagttgctc cacgtccagt tgggctgaca ctccggttgt
     7501 cccgatacca acattaccct ctaccaaaag accattggtc ggggcgagat tagtgccgga
     7561 gtaagtcgca ccgataaccg ctccaccttc aacgtctaat ttgttggccg ggttgttagt
     7621 tcctattcca acatttacgg ctcggattaa aaactggttg tctgccgtgg aggcgaaatc
     7681 ggcatcggcg ctgctatccg cccaaacaaa agtgcagagg tgatcggcct ttgcccgccg
     7741 accagcggca aaagaaaaat ctccactggc ggtgttgcta agccctccac caacggtggc
     7801 gaatctcccg ctggcgatgt tgctaaaccc cccaccaatg gtggcacggc tcccgctggc
     7861 ggtgttgaga attcccccac caacggtggc gccacccccg ctggcggtgt tggtattccc
     7921 cccaccaacg gtggcgtcgt ccccactggc ggtgttgcta aaccccccac caaccgtggc
     7981 atgtgttcta tcgctggttg tgccagcgtt gtctccagct tgattggcat cgccccctcc
     8041 tatcgttcca aagtcatcgg taacctggtt gttggtgaat gcaatcgtcc ctccgccgcc
     8101 aatagtagcg ccgcgaacac cggaagtaac gctgttgcca ctttggccgc caattaagtt
     8161 aggtgaagtg gtattcggct ctatgcgcaa ggcgcggctg ccgttcactt tcaattccag
     8221 ggcctgattg tcggtagtgc ccaaaaagtt ggtgcccgga gttgtgccac tgttgccggt
     8281 aagactccag ccacttccag cgcccgccgc cagccaggtg ccaacgccga ccgcattcga
     8341 agtcaaaaca aaaccattgg aagcgccggt cggcaattta aaaccggtca tctgggccgt
     8401 gccagcaacg tccaatttct gggctggact cgccgtcccg atgccgacgt cgccggagac
     8461 ggttaagtca ctctggatgg ccgtatttcc cgaaatggtt ccgccggtag ctccatcaac
     8521 cgttgccacg cgcagggaat aggccacggc ggtgatctgc tggcgggggg tcatttcgcc
     8581 gtccgtattc acctccacgc caaggtagcg gggagtggcc gcaaacaggt ttgccggaaa
     8641 agcgaccggc ggtgtacctg cacccagaat gacgttgtag tcgccgttgg cgtccggggt
     8701 gacagggtgc gattcgggtc cccacaaaag cgttccgcct gccggagcgt cgtagatgcg
     8761 gaacttcaac gtgaccgccg aagtaatcgg cgcaccggag ttatcagtca atttcccctg
     8821 gtagttgatg agttggggga cggcggcgcc tgcgttcacc accaaaaagg cagatacagc
     8881 cagcgtaacg ataagggtca tgagtgtcat tcggatggtt cgactccctc gatttccttc
     8941 gactgcgctc aggacaagtc gctcgggaca agtacgctca ccattaaaac aggacataag
     9001 tccccctttc aggtaaaggg tgttacgggc gaacagcgga cggatggtca tgatttgccg
     9061 gagatgaaaa cggtttgcgt tacacataca cgttcctcct cccgtgccac aacggcacag
     9121 gtaacaaaaa cgttgggcga ggtcaggaaa cctcgcacta ccacagtatc ttctgagcac
     9181 ccttaagccc tgcccttgac ccggtttgaa ggtatcctcc cccacgattt tgtcaagcca
     9241 aaattagtcc
//
LOCUS       DBNGZD010000254         3436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1638064, whole genome shotgun sequence.
ACCESSION   DBNGZD010000254 DBNGZD010000000
VERSION     DBNGZD010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1638064"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1466)
                     /locus_tag="AC16GM_06710"
     CDS             complement(267..1466)
                     /locus_tag="AC16GM_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HIR2923402.1"
                     /translation="MANYNSRTDYDIIVVGAGPGGSLAAKSAAEKGARVLLMEKHAAV
                     GYPLCCAEAISTTGLTACVPLNPRWIASKVERVVLIGPGGAKLFIQHPEAGFVLDRKI
                     FDRDLASYAGVAGAEVKVNCQAVGLIPNNGTGYAGVRAVENDGEKEYKAKVIIGADGV
                     ESQVGRWAKMNTTLPLGGIDACAQYLLGGLDIPEDLLEFYFGKNVAPGGYIWVFPKGN
                     GTANVGVGIMPSQVKGKTAKEFLDDFVSRRFPNAKILELMVGGVPMFRGRKFMQKDNI
                     LLVGDAARLVDSLSGGGIANAMLSGQVAGEVAAEFVQNGYRSFDYLKEYPQRFMKTKG
                     RELRYYLYARHIFTKMKDDDLDFTLDFLNPLFDGQPVSALEPIKTIKQVLKKRPSLLL
                     YARHIRW"
     gene            complement(1441..1671)
                     /locus_tag="AC16GM_06715"
     CDS             complement(1441..1671)
                     /locus_tag="AC16GM_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR2923403.1"
                     /translation="MAPKARKADPNKVVIFKPDLCGHCGACVAVCGFDSLVLYRTGIA
                     VDMNTCILCDACVRTCPTFALEITNGKLQFSH"
     gene            complement(1822..3141)
                     /locus_tag="AC16GM_06720"
     CDS             complement(1822..3141)
                     /locus_tag="AC16GM_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR2923404.1"
                     /translation="MSKLRHRAKSVLNAVARWLTQLRVNYEFLFIRRCLAFFIDAAIL
                     LAFSLAVAFVNFFAGYNVLLPETAKIVLPSYFLLSRFISNGRTIGMRTFRLHFAFEVM
                     QFRRVRLLYRFLLSVALLLLPVEFIPIVEYLLPNGFSKFHLTLLSFGMLSSIPIYSFL
                     MIMFSNGSASLVDRLSKCQISSRSTPSDGLTTKARGQFIRRHVGGFLLLGSLICFLLL
                     LINWRLELQKVFSDRTFAHAQLNPIERTGLTPVFQRFSKTGYPVFSLITKRSDYLIPY
                     PTEEKHDMLEVLLYIPERQFDNKELHQRIVSEVEITRRLYNHLFRANIYQSRLTLRRT
                     GVFGSFIFTSEYKYIIAPTQDRKIVFIGDIPAKVKNQILENLSLHKLNGLWYDSLWFT
                     APYPGFFEAKPEKQFIVEFSSGLMPSVFGSGFAFLISPDGSLAMRED"
     gene            complement(3116..>3436)
                     /locus_tag="AC16GM_06725"
     CDS             complement(3116..>3436)
                     /locus_tag="AC16GM_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923405.1"
                     /translation="TDPQNGIITFGGIEKIPPQSSLIITIWGETRQYLIDDPISVTYD
                     GGSGKVVRTSTVRGFASFIYENTTYLLILLALVNIGAFFAVIDAVSKKMKKVQQSEQA
                     AAPS"
BASE COUNT          897 a          823 c          907 g          809 t
ORIGIN      
        1 acagagccac aggtgaacga tgtgagaaag ggacatagaa cctccgtggt cggtacgact
       61 cctcaagaat gggtcgtttc cgacagttaa tgcagaccag ccggtccaga cgctcatcgt
      121 aatgaactct cctcgcgagc cccctccttg cgctctcgct gaaagtaatt atatgaaaat
      181 ctattaacct gtcaagctct gaaggtcagc gggcgggata ccaaactccc gctgtagggt
      241 gaggatgaat tcttcgacag tatgctttac caccggatat gccttgcgta gaggaggagg
      301 gaggggcgct tttttaagac ctgcttgatg gttttgatcg gctctagggc agagaccggt
      361 tggccgtcga agagggggtt gagaaaatcg agggtgaagt ccaagtcatc gtctttcatt
      421 ttggtaaaaa tgtgccgggc gtagaggtag tagcgcagtt cccggccttt tgttttcata
      481 aatctctggg gatactcctt caggtagtca aaggagcggt agccgttctg aacaaactcc
      541 gcagcgactt cgcccgccac ctggccggag agcatagcgt tggcgatccc gccgccggag
      601 agggaatcga ccaaacgggc ggcgtcccca acgagcaaga tgttgtcttt ctgcatgaac
      661 ttccgcccgc ggaacatcgg gacgccgccc accatcagtt ccagaatctt tgcgttcgga
      721 aagcggcggg aaacaaaatc atctaaaaac tctttggcgg ttttgccttt cacctgggag
      781 ggcataatcc ccacgccgac gttggcggtg ccgttcccct tgggaaagac ccagatgtag
      841 ccgccgggcg cgacgttttt cccgaaatag aattcgagca ggtcttccgg aatatcgagt
      901 ccacccagca aatactgggc gcaggcgtcg atgccgccta agggcagggt agtattcatc
      961 ttggcccagc ggccgacctg cgattccacg ccgtcggcgc cgatgatcac tttcgctttg
     1021 tactcttttt cgccgtcgtt ttcaacggcg cgcacccccg cgtaaccggt tccattattt
     1081 ggaatcaagc cgaccgcctg gcaattcact ttcacttccg cgccagccac accggcatac
     1141 gaggcgagat cgcggtcaaa aatcttccgg tcgaggacga agccggcttc cggatgctgg
     1201 atgaataatt tcgcgccgcc cgggccgatc aggaccaccc gttcgacctt gctggctatc
     1261 cagcgcggat tcaaagggac gcaagcagtc aggccggtgg tgctgatcgc ttcggcgcag
     1321 cagaggggat agccgaccgc ggcgtgcttt tccatcaaga gcacgcgggc gcctttttcc
     1381 gcagcacttt tcgcggcgag cgacccgccg ggaccggcgc cgacgacaat gatgtcgtaa
     1441 tcagtgcgag aattgtagtt tgccattcgt aatttccagc gcaaaggtgg ggcaggtgcg
     1501 gacgcaggcg tcgcagagaa tgcaggtgtt catatcgacg gcgatgccgg tgcggtagag
     1561 aacgagggaa tcaaacccgc agaccgccac gcaggcgccg cagtggccgc agaggtcggg
     1621 cttgaagatt acgactttgt tcggatcggc tttccgtgcc ttgggcgcca tgggctctct
     1681 cgtctctgca aaacatagag tccgaagcga caagctgtca acagcgggtt gcattcggat
     1741 agggagttgt tactacgctt aaccggttga agcggagcga actgcttagc tgtctttctc
     1801 gttacatcaa atgctaattt gttaatcctc gcgcatcgct aaagacccat ccggagagat
     1861 aagaaaagcg aaaccggaac caaaaacgga tggcataagt ccactagaaa attcaacaat
     1921 aaactgtttt tccggtttag cctcaaaaaa accgggatac ggtgcggtga accacaagga
     1981 gtcgtaccat agaccgttga gtttatgtaa ggataagttc tcaagaatct ggttttttac
     2041 ttttgctgga atgtcaccta tgaatacaat tttgcggtct tgtgttggcg cgataatgta
     2101 cttgtattca gacgtaaaaa taaatgatcc aaagactcct gtcctacgaa gagttaatcg
     2161 tgactgatag atgtttgcac ggaaaaggtg gttgtacaat ctcctcgtaa tttcaacttc
     2221 agaaacaatc cgttgatgga gctctttgtt gtcgaattgc ctttcgggga tatataacaa
     2281 cacctcaagc atgtcgtgct tttcctccgt gggatatgga atcagataat cgcttcgctt
     2341 cgtgatcaat gaaaacactg ggtaacctgt tttggagaat cgctgaaaca ctggtgtgag
     2401 gccagttctc tcaattggat ttagctgggc atgtgcaaaa gttctgtcag aaaagacttt
     2461 ttgcaattcg agccgccagt tgatgagcaa aagaagaaaa catatcagag atcctagcag
     2521 tagaaaacca ccgacgtgcc ttctaataaa ttggcctcga gccttagtag tcaaaccatc
     2581 tgatggcgtt gatcgagagg agatttggca tttcgagaga cgatccacga gagatgctga
     2641 accattggag aacataatca ttagaaagct gtatatcggg attgaactca gcatgccaaa
     2701 agagagaaga gtcaaatgga atttgctgaa gccattaggg agcagatatt caacaatggg
     2761 aatgaattcg actgggagga gcaatagtgc tacgcttagc aaaaacctat acaaaagtcg
     2821 aacgcgccta aattgcataa cttcaaaggc aaaatgaagt ctaaacgttc tcatgccgat
     2881 cgttctcccg ttagatatga atcgactcag cagaaagtag gacggcagaa cgattttagc
     2941 ggtctctggc aggaggacat tgtaaccagc aaagaaattt acaaaggcca cagcgaggga
     3001 gaaagcgagt aggattgcag catcaatgaa gaaagcgaga caccgacgga taaacaggaa
     3061 ttcgtagtta actctgagtt gcgtcaacca tcgagcaact gcattcagaa cggacttagc
     3121 tcggtgccgc agcttgctca ctttgctgta cctttttcat ttttttgctg accgcatcaa
     3181 tcacagcgaa aaaagctccg atgtttacta aggctaaaag aataagaagg taggtcgtgt
     3241 tttcgtagat aaaggatgcg aatcctctta cggttgacgt tcggacaact ttgcccgacc
     3301 caccgtcata ggtgacgcta attggatcgt caatcaaata ttgacgagtt tcaccccaga
     3361 tcgtaataat gagcgatgat tgaggtggaa ttttctctat acctccaaat gttataatcc
     3421 cattttgagg gtctgt
//
LOCUS       DBNGZD010000255         4286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_110046, whole genome shotgun sequence.
ACCESSION   DBNGZD010000255 DBNGZD010000000
VERSION     DBNGZD010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_110046"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..128
                     /locus_tag="AC16GM_06730"
                     /pseudo
     CDS             1..128
                     /locus_tag="AC16GM_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            complement(220..2064)
                     /locus_tag="AC16GM_06735"
     CDS             complement(220..2064)
                     /locus_tag="AC16GM_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2923406.1"
                     /translation="MESNQLIGQMLGKYRIDAKLGEGGMGVVYRATDITLDRVVALKM
                     LQKEITVDPKIKERLKIEAKALARFNHPNIAILYEFDVVNNFFAMEYIEGQTVEELCK
                     QEGVLPPERIRSILKQVLPALGVAHKSGIIHRDIKPANLMLTPSGLVKITDFGIAKMP
                     AGVSGVKTMSGERIVGSYYYISPEQIQHMPVDGRTDIYSLGVTLFEMATGKVPFDSDS
                     DFLMMKAHVEDPPPLPSSLNPHLDPRFEKVILKALEKDPKNRYQSAEEMLVALDAAVL
                     SPKPVPNPPGKLAEERKSASRRRRWLVPVGLLFVVGLIAAAVMVFSKGKNNGTPEFFG
                     GAEPSAQITLSDVPAGGQVFLDDEKVLVTPDQPLELAPGTHRLRVEKSGALPWNRKFE
                     VEPGQRLAFSVALAVNQGFLRVVTNPPEAELSLDDLPIKNVAEALPVTVGAHRLKAQA
                     PGFRDTIAEFTVESGETKSVALTLNSRGEFLRITNLPPEAYVWVNGQEVRNAGEPLRV
                     PFGENRIHVRVPEREDWSRLVMVPAGKMVSVEYQPPKGMLTLSARPYATFYVDGQKIG
                     ESGVKTQLEVEAGTHQVRLVYLDGSFDTTVVVSSKTPTEVNHTFRVRR"
     gene            2421..3908
                     /locus_tag="AC16GM_06740"
     CDS             2421..3908
                     /locus_tag="AC16GM_06740"
                     /inference="COORDINATES: protein motif:HMM:NF025067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR2923407.1"
                     /translation="MKHGFGILGLALLLGLLGDLLLRATPWGVNIFFWAIALLLSVIL
                     LIHRWRVELAGDVLRFALPVVFFAFGFVWRDSLTLKALDALALLVILSLFTMRSQAIQ
                     LRLSGLVRYLEGFVLTGINLAAGIFPLVLADIAWKDIPRGGWSRQSVAVLRGLLIALP
                     LLLVFGSLLMAADAVFAGIIKNLLHFNLADVFKHIFVTGLAAWPVGGFLRGIFLADKQ
                     TMFSGELPKSPGLGIVEMGVALGSLNVLFLGFVFVQFRYFFGGAEVVTLTSGLTYAEY
                     ARRGFFELVVAAALVLPLLLFGHWLLRKDQPTYEKVFRLLAGMLIVMLFVVLASAFQR
                     MHLYQQEYGLTELRLYTTAFMGWLAVVFIWFGATVLRGKGERFAFGAVVAGFLTIVVL
                     SSFNPEALIIRTNAALAKTGKSFDVDYALSLSADAVPSLVATLPDLDPKDRCNVATLL
                     LKNWSPSTHTDWRTWNWGQARARRVVEKNQDDLQEMVKSWFIQNF"
     gene            complement(3922..>4286)
                     /locus_tag="AC16GM_06745"
     CDS             complement(3922..>4286)
                     /locus_tag="AC16GM_06745"
                     /inference="COORDINATES: protein motif:HMM:NF040055.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR2923408.1"
                     /translation="RGRVGRGPARSYCILLSYGALSLEAHQRLEILQQTEDGFKIAEA
                     DLKLRGPGEFFGTRQHGLPELKIADLIGDQKLLFDARRWAQRLWQEDPNLAHPKHQRL
                     REQFTRQYHIKLDYLRAG"
BASE COUNT          768 a         1267 c         1230 g         1021 t
ORIGIN      
        1 aggtggtgta caagaaactg gtcgggatgc agatgctggt cgaagggctg gcgatgggcg
       61 cgttcgccac cctgcacgtc aagtcgcccg atccgctgct gcgtcggctg tgccagctga
      121 ctatgacatg accgtgctga tgaaaaaatg tgcactcacc agccacagcg ccgttttcct
      181 gagccgggaa tctatccgta gcattttgcc tccttttcgc tatcgcctca cgcgaaacgt
      241 atggttgact tccgtgggcg ttttgctgct caccactacc gtggtgtcga agctgccgtc
      301 caggtacacc aggcgtacct ggtgggttcc cgcttccact tccaactgcg tttttacccc
      361 ggattctccg atcttctgac catcgacgta aaacgtcgcg tacggccggg ccgaaagcgt
      421 gagcatcccc ttggggggct ggtattcgac cgacaccatc ttccctgccg ggaccatcac
      481 cagtcgcgac cagtcttccc gctccggcac ccgcacgtgg atacggttct ccccgaacgg
      541 cacccgtaac ggttcccccg cgttccgtac ttcctggccg ttcacccaga cgtatgcctc
      601 cgggggcaga ttcgtgatcc ggaggaactc gccccgcgag ttgagggtga gcgccacact
      661 cttcgtctcg cccgattcga cggtgaactc cgcgattgta tcccggaacc ccggcgcctg
      721 ggctttcagc cggtgggctc ccacggtgac cggcagcgct tcggctacgt tttttatcgg
      781 cagatcgtcg agacttaatt cggcctcggg cggattggtc accacccgca ggaacccctg
      841 attgacggcc agagccacgc taaaggccag gcgctgcccc ggttccactt cgaattttct
      901 gttccaggga agcgcgcccg atttttccac ccgcaaccgg tgcgttccgg gggccagttc
      961 cagcggctgg tccggagtta ccaagacctt ttcatcgtcg aggaagacct ggcccccggc
     1021 gggcacgtcg ctcagggtga tctgggcgga gggctcggcg ccgccgaaaa actccggagt
     1081 gccattgttc tttcctttgg agaagaccat gaccgcggcc gcaatcagcc cgaccacgaa
     1141 gagcaagcca accggaacca gccagcgcct ccgccgggaa gcgcttttcc tttcctccgc
     1201 caatttcccg ggggggttcg gcaccggctt ggggctcagc accgctgcat ccagagctac
     1261 cagcatttct tccgcagact gatagcgatt cttcggatcc ttttccagcg ctttcagaat
     1321 cactttttca aagcggggat caaggtgggg attgagggaa gaaggcaaag gcggcgggtc
     1381 ctccacgtgc gccttcatca tcagaaaatc gctgtccgaa tcgaacggca cctttcccgt
     1441 cgccatctcg aaaagggtga cgcccagaga gtaaatgtcg gtccgcccgt ccaccggcat
     1501 gtgctggatc tgctccggag agatgtagta gtaggaaccg acgatccgtt cgccggacat
     1561 ggtcttgacg ccggagacgc ccgccggcat cttggcgatc ccgaaatcgg tgatcttcac
     1621 cagcccgctg ggggtgagca tcaggttggc cggcttgatg tcgcggtgga tgatccccga
     1681 cttgtgggcc acacctaagg cgggcaaaac ctgcttgagg atggagcgga ttctctccgg
     1741 cgggagaaca ccctcctgtt tgcacagctc ctccaccgtc tggccttcga tgtactccat
     1801 ggcaaagaag ttgttcacga cgtcgaactc gtagaggatg gcgatgttgg ggtggttgaa
     1861 gcgggcgagc gctttggcct cgatcttcag ccgctctttg attttcggat cgaccgtgat
     1921 ctccttctgg agcatcttga gcgccaccac ccggtccagg gtgatgtcgg tggcccggta
     1981 gaccacaccc atccccccct cgcctaattt ggcatcaatc cggtacttgc cgagcatctg
     2041 gccgattagc tggttacttt ccaccttatt tcggattatc gacgcaaagc cgaaagacgt
     2101 tactttgcag ggaatcagcg gtttcgccct gatagggaag tttgtttgct ggcagtttat
     2161 ttgtgaggga gaaactgttc ctctcctttg cgggtcgaag atccagccgc ggcggaggaa
     2221 ggtggggaga ggtcagccct gcttttccct ccccttggag gggagggtag ggaggggtca
     2281 gcaatccatg tagaattgtt ggatttgaga actaacgtag aaataagcct gactacccaa
     2341 atgccatctc tcgccgataa ggtaattgaa cgtctttacc caacgtataa tgggagagca
     2401 ggtcgccaat gaacgaccgg atgaaacacg gatttggcat tctcggactg gctcttttgc
     2461 tgggcctact cggcgaccta ttgctccgcg ccacgccctg gggcgtgaac atttttttct
     2521 gggcgattgc actgctgctc tcggtcatac tgctcatcca tcggtggcgt gttgaactgg
     2581 ccggggatgt gctccggttc gctctacccg tcgttttctt cgccttcggt ttcgtctggc
     2641 gcgactcatt gactctaaag gctctggacg ccttggcact tctggtcatc ctgtcgcttt
     2701 ttacgatgcg ctcacaggcg attcagctcc gcctttccgg cttggtacgc tacctggagg
     2761 gcttcgttct caccggaatc aacctggctg ctgggatttt ccccctggtt ttggccgata
     2821 tcgcttggaa ggatattccg cgcggcggat ggtccaggca gtcagtggcc gtattgcgcg
     2881 gcttgctcat tgccttaccg ttgctgctcg tattcggaag tctcctaatg gcagccgacg
     2941 cggttttcgc ggggataata aaaaaccttc tgcactttaa tcttgcggat gtgttcaagc
     3001 atatctttgt aacgggcctc gctgcgtggc cggtcggcgg gttcttgcgc ggcatctttt
     3061 tggccgacaa acaaacgatg ttcagcggtg aactccccaa atcccccggg ctgggcatcg
     3121 tggaaatggg tgtggcgctg ggttcgctga atgtcctttt cctcggtttc gtgttcgtgc
     3181 agttccgtta cttcttcggc ggtgccgagg tggtcaccct cacttccgga ctcacttatg
     3241 ccgagtacgc ccggcgtgga tttttcgaac tggtggtcgc tgccgcttta gtcttgccgc
     3301 ttttgctctt cgggcactgg ctcttgcgta aagaccaacc cacctatgag aaggtattcc
     3361 gccttctggc ggggatgtta attgtcatgc tctttgttgt cctggcttcc gccttccagc
     3421 gcatgcatct ctatcaacag gagtacgggc tgactgaact ccggctctac actacggcct
     3481 tcatgggctg gttggcggtg gtctttatct ggtttggtgc tactgtgctc cggggaaaag
     3541 gggaaagatt tgcttttggt gcagtggtgg cgggttttct gactattgtc gtgctttctt
     3601 ccttcaaccc ggaggcactc atcatccgga ccaacgccgc cttggccaaa accggcaaga
     3661 gcttcgatgt ggactatgcc ctctcattaa gcgcggacgc ggttccctcc ctggtggcga
     3721 ctctgcccga ccttgacccc aaagatcgct gtaacgtggc aaccctcctg ctcaaaaact
     3781 ggtctccgtc cacgcacacc gattggcgca cctggaactg gggtcaggcg agagcccgcc
     3841 gggtagtaga gaaaaatcag gacgatttgc aggagatggt aaagtcctgg tttatccaaa
     3901 acttttgaac cttgaaaaga ttcacccagc ccgcaggtaa tctaacttta tatggtactg
     3961 gcgggtgaac tgctcccgca ggcgctggtg tttggggtga gccaagttcg ggtcttcctg
     4021 ccagagcctc tgcgcccagc gccgggcgtc aaatagaagt ttttgatccc cgattaggtc
     4081 ggcgattttc aattccggca agccgtgctg tcgcgtcccg aagaactccc ccggcccgcg
     4141 cagctttaaa tcggcctcgg cgattttgaa cccgtcctca gtttgctgca gaatctcgag
     4201 ccgctggtgg gcttccagcg aaagggcgcc gtaggaaagc agaatgcagt acgaccgggc
     4261 cgggccgcgc cccacccgcc cgcgca
//
LOCUS       DBNGZD010000256         5869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_110425, whole genome shotgun sequence.
ACCESSION   DBNGZD010000256 DBNGZD010000000
VERSION     DBNGZD010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_110425"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC16GM_06750"
     CDS             <1..552
                     /locus_tag="AC16GM_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2923409.1"
                     /translation="TVDTRDRTPFPTRGNHHEVRFTYAGGPLGSDVRYRSAAGFFENF
                     FPLARRTVINSRLALGFSGSAIPLPDQFRLGGAIAPLAGFASGGTAAPLGGAFRDEFT
                     GPQLFAASLGLRHKMGDRLYLGARGDLGNVWEKTGQMRFSDLRRGALLSGAVGTPVGP
                     LEVDLGFTQRQKPRLYFFWGHQF"
     gene            complement(651..1547)
                     /locus_tag="AC16GM_06755"
     CDS             complement(651..1547)
                     /locus_tag="AC16GM_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR2923410.1"
                     /translation="MLDFFAIAALCLVWGGTWLAIKIGLSDAPPFLSAAARFLIACLL
                     LFPFIWRKKLALPKTKKEISAILVTGFCMYALSYALVYWAEQYVPSGLTAIIFSTLPF
                     LVALFAHFLLPAEPFTLPKTVGMVVGFAGIGLIFWEGAGIAGSRQFLGMLLLLGSPLA
                     TSYANVLTKRDLHDVHPAVIAGLQMALGFVLLSVLGFAFERFADFHLTHRSLGSLAYL
                     ATFGSVFAFATYYWLLKRVEATKLSLIAFVTPVVALIVGALFGDEHLSAKIASGSALV
                     VLGIAIATQAKIFSKNQPERWW"
     gene            complement(1560..2027)
                     /locus_tag="AC16GM_06760"
     CDS             complement(1560..2027)
                     /locus_tag="AC16GM_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923411.1"
                     /translation="MQQIDVKDTVLIVVGVELNAEEKDRPIAYYLKSQIDKHARAAGK
                     PYRKGIVITDALWLRDKIVQICPTIALGGPGVNALAAQLVDKLPVAYGEENRFFIQME
                     EQVLNNKISIWGMNQATTKMGVDTFLSRGLLDKYLEVVWPSGSTRVESDPSGK"
     gene            2444..4150
                     /locus_tag="AC16GM_06765"
     CDS             2444..4150
                     /locus_tag="AC16GM_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2923412.1"
                     /translation="MRSGASVSGTAMRWCGLFLIFFLAGNLAAQTGRKVKMEPGGSLR
                     LFLSAERVELAALAESVSFPVNTLQADNLTIRSGQRVVADKDGFHMNVQIFAAGQVSE
                     IVVGKKKDSVVTKHPSEDDTFSLFSAFSVGPDDFLRGDLFLFGGSAAVAGEISGDIIA
                     LFGSVRLEPTAVVRGDVVAIGGSVEVAEGAMTYGEITTVRSSDNKVSTISHRKRGIRF
                     RFDSRFDPGFTYNRVDGVALFPRYTFQGKPNGRPTVHLAGGRAFERNRYLYDFGFKEE
                     IGDSNTFLFGGSFYRYTATDDDWILPAGENNVSTLFVRRDFRDYVEREGINGFVGQKL
                     FRYHTVRLDYFFDRYRPLRNASNWSLFGGPKRFRANFSRVPDTLLVRYAGDFNAEIGS
                     AIFTYAFDTRDDEDDPTRGWLGKLEIEHGGPDLGGDLFYTRWRAVLQRYQPITLDQNL
                     SLRAMYGNSGERLPLQRLFFLGGLGTMRGYEFKEFSGDQFALLNADYDVRLPKGSLTA
                     VLFFDLGTATFRRNLFDAGPWKADVGLGLKLGSVVRFDLAKRLDDFDRNLKLTVRFAR
                     MF"
     gene            complement(4264..4617)
                     /locus_tag="AC16GM_06770"
     CDS             complement(4264..4617)
                     /locus_tag="AC16GM_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HIR2923413.1"
                     /translation="MKKFVEIRSLNLKPGTRGEFHRRYLEKALPLLNKWKMNVVAFGP
                     SPHDENSYYVIRAFESLEHRQESEETYYGSSDWRQGPREEMLALIENYIDIVIEMEEE
                     TVNALRESSTPYSLP"
     gene            complement(4620..5135)
                     /locus_tag="AC16GM_06775"
     CDS             complement(4620..5135)
                     /locus_tag="AC16GM_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HIR2923414.1"
                     /translation="MKNRLSAMAGVVLCISFAFGLSPTAQAQVPDTGKKEALVTKKAC
                     GTFEVKLTRQPTADSAGQTKISRMAFDKKFSGDLQATSKGEMLAAGTGAPGSSGGYVA
                     IKQVSGSLNGKKGSFILQHSGTMRRGAPELVITVVPDSGADELKGLAGKMEIKIEEGK
                     HSYVFEYTLPE"
     gene            complement(5132..5638)
                     /locus_tag="AC16GM_06780"
     CDS             complement(5132..5638)
                     /locus_tag="AC16GM_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2923415.1"
                     /translation="MKKTYRKGGVGAMMDEYERAAGELKRVLAQTSEENFNRIVDTET
                     KDEHCRSIQTIMTHVVGAGYGYADSIRKAISMPSARPKETFRDTLVPVQHREAAAKLD
                     AMLAYTAQTLEGRWEMTDEEIERMTLKTSWGATYHLEQLLEHAIVHILRHRRQVERFL
                     ERMAKEGI"
BASE COUNT         1167 a         1660 c         1758 g         1284 t
ORIGIN      
        1 accgtggata cgcgcgaccg cacgcctttt cccacccgcg ggaatcacca cgaggtccgt
       61 ttcacctacg cgggcggccc tttgggttcc gacgtacgct accgtagcgc agccggcttt
      121 ttcgaaaatt tctttccttt ggcccggcgg acggtgatca attcacggct ggcgctgggc
      181 ttttccggca gtgcgatccc tctgcctgat cagttccggt tgggaggcgc gattgcaccc
      241 ttagccggct tcgcttccgg cggcacggcg gcgcccctgg gtggggcttt ccgggacgag
      301 ttcacgggcc cgcagctctt cgcagccagt ctcggcctgc ggcacaaaat gggcgacaga
      361 ctttacctcg gcgcgcgcgg cgacttaggc aatgtctggg aaaaaacagg gcagatgcgg
      421 ttttccgatc tccggcgggg cgccttgctt tccggcgcgg tggggactcc ggtcggacca
      481 ctggaagtcg atttaggttt tacccaaaga caaaagccgc ggctctactt cttctggggg
      541 caccagttct gaggcgctgc cgcagagccg atttgggcga ccgctacggc cgtatggtga
      601 cgggtcgtcc ctaccggaaa acgacgcgtt atttccataa aaacgaaacg tcaccaccag
      661 cgttcgggtt gatttttcga gaatattttg gcctgcgtgg caatagcaat gcccagcacc
      721 accagagccg agccagaggc gattttggcc gagaggtgct cgtcgccgaa tagcgcaccc
      781 acgatcaacg ccactaccgg ggtcacgaaa gcgatgaggg agagtttggt cgcctccacc
      841 cgcttcagga gccagtagta ggtcgcaaag gcgaagaccg aaccaaaggt cgccaggtag
      901 gccagggagc cgagtgaccg gtgggtgaga tggaaatcgg cgaaacgttc aaacgcgaaa
      961 cccagaacgg aaagcaaaac gaatcccaga gccatctgga gtccggcgat cactgccgga
     1021 tgtacgtcgt gcaggtcgcg cttggtcagg acgttggcgt aggaggtcgc taaagggctg
     1081 ccgaggagga ggagcatccc cagaaactgc cgggagccgg cgatcccggc tccttcccag
     1141 aagatgagtc cgatgcccgc aaaaccgacg accatgccga ccgttttggg cagggtgaac
     1201 ggctcggcgg gcaaaaggaa gtgggcgaag agcgccacca agaaaggcaa agtcgagaag
     1261 atgatcgcgg tcagcccgga ggggacgtac tgctcggccc agtagaccaa tgcgtaggag
     1321 agggcgtaca tgcaaaagcc ggtgaccaga atggcggaga tttccttttt cgttttcggg
     1381 agggccagtt ttttgcgcca gatgaaaggg aagaggagga ggcaggcaat caaaaaacgc
     1441 gccgccgcgc tgagaaaagg cggcgcatcg gataagccga tttttatcgc cagccaggtg
     1501 ccgccccaga ccaaacagag tgcggcaatg gcgaagaagt cgagcattta gggaggagac
     1561 tacttgcccg acgggtccga ttcgactcgt gtcgagccgg agggccagac tacttcgagg
     1621 tacttgtcca gaagcccccg ggagaggaag gtatcgaccc ccattttggt cgtggcctga
     1681 ttcatccccc agatggaaat tttgttgttc aggacctgct cttccatctg gatgaagaag
     1741 cggttctctt cgccgtaggc caccggcagt ttgtccacca actgtgcggc cagggcgttc
     1801 acccccggac cgcccaaggc gatggtcggg caaatctgaa cgattttgtc ccgcagccag
     1861 agggcgtcgg tgatgacgat gcccttgcgg tacggtttgc ccgccgcgcg cgcatgcttg
     1921 tcgatctggc ttttgaggta gtaggcaatg ggccggtcct tctcttcggc gttcagttcg
     1981 acccccacca cgatcagaac ggtatccttg acatcaatct gctgcacggg gaactcccag
     2041 cgaatatttt ctcatagaca aatccagaca ggcaaataag gattttccga gaggatgtca
     2101 aacaacagga cggatttggg atgagggggt taggatttgg ggaaatatga gaggggagtt
     2161 cgacccggat agaaagggta acaaaacgga ggagaaattc cggccacggg aattcggtca
     2221 ggctggtcag acacgactct accgagaagc ctgacctatg gatgagagac atttctacct
     2281 ccatagccga ggtttctagc ctcggaacgg ccacaagggc ctatgcctat gcgtggaggt
     2341 agtagatcaa tgcaccttag ccaggagggc ataactcaga acgcgcactt ggacttcagg
     2401 gaagggctct tgtattatat accgagcgag ctatggaggg ccggtgagaa gcggagcatc
     2461 tgttagcggc acagccatgc gctggtgtgg attgtttttg attttttttc tggcgggcaa
     2521 cctcgccgcc cagaccgggc ggaaagtgaa aatggagccc gggggatctc tgcgtctttt
     2581 tctttcggcg gagcgggtgg aactcgccgc gctggcggag agcgtctctt ttccggttaa
     2641 taccctgcag gccgataacc tgacgatccg ctccggtcag agagtggtag ccgacaagga
     2701 cggctttcac atgaatgtgc agatattcgc ggcggggcag gtgtcggaaa tcgtcgtggg
     2761 gaagaaaaaa gattccgtcg tcaccaaaca tccgtcggaa gacgacacct tttcgctctt
     2821 cagcgcgttc tccgtcggcc cggacgactt tttgcgcggc gatctcttcc tcttcggcgg
     2881 gagtgccgcc gtcgcggggg agatctccgg cgacatcatc gcgctgttcg gctcggtacg
     2941 gctggaaccg acggcggtcg tccggggtga cgtggtggct atcgggggca gtgtggaggt
     3001 ggccgagggt gccatgacct acggggaaat cacgacggtg cgctcctccg acaacaaagt
     3061 ctccaccatc agccaccgca agagaggcat acgcttccgg ttcgactcgc gttttgatcc
     3121 gggctttacc tacaaccggg tcgacggtgt ggcgcttttc ccgcgctaca ccttccaggg
     3181 caagccgaac gggcgcccca ccgtccacct ggccgggggc cgggcctttg agcgcaaccg
     3241 gtatctgtac gatttcgggt tcaaagagga aattggcgac agcaatacct tccttttcgg
     3301 cgggagtttc taccgctaca ccgccaccga tgacgactgg attctgccgg cgggggagaa
     3361 taacgtctcc acgctctttg tgcggcgcga cttccgggat tatgtcgagc gagaggggat
     3421 caacggtttt gtcggccaga aattattccg ctatcacacc gtccgcttag actacttctt
     3481 cgaccggtac cgtcccttgc ggaacgcgag caactggagt ctcttcggcg ggcccaagcg
     3541 gttccgcgcc aacttttcgc gcgttcccga tacacttctg gtgcgctacg ccggggattt
     3601 caacgctgaa attggctcag ccattttcac ctacgctttc gatacgcgtg acgatgaaga
     3661 cgatcccacc cggggttggc tgggcaaatt agaaatcgag cacggcggcc cggatctcgg
     3721 gggcgatttg ttctacaccc gctggcgcgc cgtcctgcag cggtaccagc cgataacctt
     3781 ggatcagaac ctgtcgctgc gggccatgta cggcaattcg ggcgagcggc tcccgttgca
     3841 gcgcttgttc ttcctcggcg gcctcgggac tatgcgcggc tacgagttca aggaattttc
     3901 aggcgaccag ttcgcccttt tgaacgccga ctacgacgtg cggctgccca aagggtcttt
     3961 gaccgccgtc ctgttcttcg atctgggcac cgccaccttc cggcggaatc tgttcgacgc
     4021 cgggccgtgg aaagccgacg tgggcctcgg cctcaaactt ggcagcgtgg tcaggttcga
     4081 tctggccaag cggctggatg acttcgaccg gaatttgaaa ctgacggtgc ggttcgcgcg
     4141 gatgttttaa gtttgagggg gacgggggcg cggcctcgtg ccccctgaac taaggggaag
     4201 gctgaccccc tccggcactc cctcgacttc gctcgggaca agccgtgcct cgtccccctg
     4261 tttttaaggg agactataag gggtagaact ttcccgcaga gcgttgacgg tttcctcttc
     4321 catttcgatg acgatgtcga tgtagttttc tatcagcgcc aacatctcct ccctcggccc
     4381 ctgccgccaa tcggagctcc cgtagtaggt ttcctcgctc tcttgacggt gttccaaact
     4441 ttcaaaggcg cggatgacat agtaggaatt ttcatcatga ggggaggggc cgaaagcgac
     4501 gacgttcatt ttccatttgt ttagcaaggg tagcgctttt tccaagtagc ggcggtgaaa
     4561 ctctccgcgg gtgcctggct ttaaatttaa agagcggatt tcaacgaatt ttttcatccc
     4621 tactccggga gggtgtactc gaagacgtag gaatgtttcc cctcttcgat tttgatttcc
     4681 atttttcccg ccagaccctt caactcgtct gcccccgagt ccggcaccac ggtgatgacc
     4741 agttccggcg cgccgcgcct catcgtgccg ctgtgctgga ggatgaaact tccctttttg
     4801 ccgttcaaac tcccgctcac ctgcttgatg gcaacgtaac cgccggagga gcccggagcg
     4861 cccgtgccag ccgcgagcat ctcccccttg ctggttgctt ggaggtcgcc ggaaaacttt
     4921 ttgtcgaagg ccatccggct gattttcgtc tgcccggcgc tgtcggccgt cggctgtcga
     4981 gttaacttta cttcaaacgt gccgcaggct tttttcgtca ccagcgcctc ctttttgccg
     5041 gtgtccggaa cctgcgcctg cgccgttggg ctgagaccaa aagcgaacga gatacacaga
     5101 acgacaccgg ccattgcgct aagccgattt ttcatattcc ctcctttgcc attctctcca
     5161 aaaatctttc cacctgcctt ctgtgacgca gaatgtgcac gatggcatgt tccaaaagct
     5221 gctccaaatg ataggttgcg ccccaactcg ttttcaaagt catccgctca atctcctcgt
     5281 ccgtcatctc ccatctccct tccagggtct gggcggtgta ggcgagcatg gcgtcgagtt
     5341 tggccgccgc ttcccgatgt tgaacgggaa cgagggtgtc gcggaaggtt tctttgggcc
     5401 gggcggaggg catggatatt gccttacgga tggaatcggc atagccgtag cccgcgccca
     5461 ccacgtgggt catgatggtt tgaatggagc ggcagtgctc gtctttggtt tcggtatcga
     5521 cgatgcggtt gaaattttcc tcggaggttt gcgccagcac gcgcttaagc tcccccgccg
     5581 cccgctcata ctcgtccatc atcgccccaa cgccgccttt acgataagtc tttttcatat
     5641 tcctggagca taagatttct cctttatccc gtcaacggtg gcgcccacgc ccaacttctg
     5701 gagctgggcg gaggcaaaca ctacggactg gtcacaaggt attatggact tgtggaaagt
     5761 ttcttttttt agtttcttgc tttttgatga cgagactaag aggggacggt gggtaaggaa
     5821 tgagtgatag tcgcaaaccg cttaagcatc ctttagccga aatctttgg
//
LOCUS       DBNGZD010000257         6719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1078189, whole genome shotgun sequence.
ACCESSION   DBNGZD010000257 DBNGZD010000000
VERSION     DBNGZD010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1078189"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="AC16GM_06785"
     CDS             complement(<1..1355)
                     /locus_tag="AC16GM_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2923416.1"
                     /translation="MRNLYYSVVLIGFSLICSKTAWGIENADCFTCHGDRSLTKNTPA
                     GQAVSLFVDQKKYERSVHGVFACVTCHHDIKEIPHPEKLAKVDCSSCHTETVEKYKQG
                     VHGMALTKGNRGSPDCGGCHTAHAVLPASDPKSPTARKNIPDLCGKCHGDLKFVEEQT
                     GIISARPFFNYKESVHGKALQKGDEQAAVCSDCHKSHDIRPPNDPGSTIFKANVPQTC
                     GRCHAAVAAEYVASIHGQALLAGISKSPVCTDCHGIHTIKARIDPKSPVAAQALARTT
                     CIQCHESEGLSREYGIPAKRISTYLDSYHGLASRFGSTVVANCASCHGIHNIFPSSDK
                     RSLVHPDNLAKTCGKCHPGASENFARGKIHVGVAQAQDVGTRVVKWVTIIYIFLIVAV
                     VGGMVLHNVLDWSRLVWGSWRKEVPGLLVKFTAFQRAQHIVLFISFFSLALTGFALKY
                     P"
     gene            complement(1452..4232)
                     /locus_tag="AC16GM_06790"
     CDS             complement(1452..4232)
                     /locus_tag="AC16GM_06790"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2923417.1"
                     /translation="MHILKHVFRVGVLLLLYSPSSSFATNADCLGCHQEKDLTKTTAE
                     GSVQSLYVDQKKFENSVHGQLACVDCHADLAQAEFPHQERVAPVDCGSCHDQVATVYR
                     GSLHGQAVAAGAKLAPRCWDCHGAHDILPVQSPESRVRRFNVPFVCGRCHKEGTAVTR
                     TYNIPQDSILTHYSVSVHGEGLFKKGLTVTAICTDCHTAHNVLPHTDPHSTIYRENVP
                     KTCEKCHGLIEQVHRKVIRGELWEKEPNKVPVCIDCHAPHRVRKVFYELGMSDRDCLR
                     CHAQPRLFTIRNGDTISLSIDTVEMRNSIHRTTACAQCHTGASPVEKRPCATITTRVD
                     CSICHAEAAAVYATSTHGTLANRGDPNAPLCRDCHGVHGTKERRDPTSPTYPTHVPNL
                     CGQCHREGEKAALRLTGEKTKIVKNYIESIHGKGLLQSGLVVTAMCTDCHTAHHELPS
                     RDSASSVNRANIAKTCARCHNGIYEQFALSIHFLGKAKTDKPLPLCHDCHTSHTITRT
                     DQEGFKLKMISQCGRCHQDVAEGYFETYHGKVSKLGYTAAAKCYDCHGAHDVLPPWDP
                     KSRLSRQNIVKTCGQCHPGSHRRFAGYLTHATHHDPVKYPVLFYTFWSMTLLLIGTLG
                     FFGLHTILWLPRSFQAMRERKKLDLQSPGKEYLRFDTLSRRLHVLVIVSFIGLALTGM
                     TLKFSYLGWAQWLSRLLGGFESAGYIHRVCAVITFFYFGTHIFDLVRKKRRERKTWKE
                     VLFGPASMLPNKIDLLEFWGTFKWFVGLGPRPAYGRWTYWEKFDYFAVFWGVTIIGAT
                     GLFLWFPETFTHIFPGRFINMATIIHSDEALLAVGFIFTVHFFNTHFRPEKFPMDTVI
                     FTGRVPLEELKLDRPREYEELVTSGELEKRLVEPLPPVVVRGMKMFGWIALSLGLLMV
                     LLIVWAEIFGYR"
     gene            complement(4255..5691)
                     /locus_tag="AC16GM_06795"
     CDS             complement(4255..5691)
                     /locus_tag="AC16GM_06795"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2923418.1"
                     /translation="MKKLFARVSRNLLSFLGLLFALVSSVLVITIVAIDLLGVQISPY
                     IGILVYLILPAFLVAGLVMVPVGLALEKRRVHLQQGLPKELALPKIELDLNRPVLRKR
                     IAWLAVLSTLFLSILATATYKAVEFMESVTFCGQVCHKVMEPELVTYSHSPHSRVACV
                     SCHIGPGAPWFVKSKLSGVGQVFAVTFNTYDRPIPTPVKNLRPARETCEQCHWPQKFH
                     GQQLRVFTHYSADEKNTALKTVLLLKTGGGDKESGIAEGIHWHMNIANEISYIATDAA
                     RQTIPWVRLKDLKGKIVEYATPDFQVSRDSLAKLPQRVMDCMDCHNRPTHVYRLPDDA
                     VDEALLTRQIDPGLPYVKKAAVELLQSEYANKPEALQKIKSGLFTYYRQNYPVLADSL
                     ALEIQKAGEKLAQVYANNVFPKMKITWGTYRSNIGHQNSSGCFRCHDDNHQSKDGQTI
                     RQDCDQCHSLLATEEENPKPVIESLPIR"
     gene            complement(5688..>6719)
                     /locus_tag="AC16GM_06800"
     CDS             complement(5688..>6719)
                     /locus_tag="AC16GM_06800"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2923419.1"
                     /translation="GYVGAPKPAQIPAFCGRCHSDVEYMKRYDPRLPTDQYAQFKTSV
                     HGKRLVRGDTLVANCVSCHGVHGILPPSDTRSPVFKTHIPGTCAHCHADTKRMASYGI
                     PTNQYDDYRQSVHGELLLDKGDRSAAACTDCHGNHGATPPGLSDVAGACGECHPANRD
                     LFEKSPHKIPFARTGLPQCATCHDNHKVLRPTDAFLGTGPEAVCIRCHSEGDKGYSSA
                     EEFKASLDSLKSAVAEARGLLERAEVAGVKTALAKFDLKASEDAVIRSRSALHSFSLA
                     DFQAITRDGMGRALQVKKAALASLEDLEIRRKGLAFSAVVILLLCIGLWLKIRRIEAR
                     QKEKAGRTQ"
BASE COUNT         1348 a         1901 c         2045 g         1425 t
ORIGIN      
        1 tccggatatt tcagcgcgaa accggtcagg gctaaagaaa agaagctgat gaagaggacg
       61 atgtgctgcg cccgctggaa ggcggtgaat ttcactaaga ggccgggtac ctctttccgc
      121 caactgcccc ataccaatcg tgaccagtct aggacgttgt ggagtaccat cccgccaacg
      181 acggcgacga tgagaaagat gtaaataatc gtcacccatt tgaccacgcg tgtgccgacg
      241 tcctgtgcct gcgccacccc cacgtggatt ttcccgcggg cgaaattttc cgacgcaccg
      301 ggatggcact tcccgcaggt cttggccagg ttatcgggat ggactaacga gcgtttgtcg
      361 gaagacggga agatgttgtg gatgccatgg caggaggcac agttggctac cacggtggaa
      421 ccgaagcgcg atgccaaccc gtgatagctg tcgaggtacg tggaaattct ttttgccgga
      481 atgccgtact cgcgcgacaa cccctcactt tcgtggcact ggatacaggt cgtccgtgcc
      541 agcgcctgcg ccgccaccgg tgacttcgga tcaattctgg ccttgatggt gtgaatgccg
      601 tggcaatcgg tgcagacggg tgatttcgat ataccggcca aaagcgcctg tccatggatg
      661 gaggccacgt actctgcggc cacggcagcg tggcaccgcc cgcaggtttg cgggacattg
      721 gccttgaaaa tcgtcgaacc ggggtcattg ggcggccgga tatcgtgact tttgtggcag
      781 tccgaacaga ctgccgcttg ctcatcccct ttttgcagcg ccttcccgtg gactgactct
      841 ttgtaattga aaaagggccg ggcggagatg atgccggttt gctcctccac gaatttcaaa
      901 tcgccgtggc atttcccgca caggtcaggg atgtttttcc gtgcggtcgg tgacttcgga
      961 tcggaggcgg gcaggaccgc atgcgcggta tggcagccgc cgcagtcggg agagccgcga
     1021 ttccctttgg ttaatgccat cccgtgcact ccctgcttat acttttcaac cgtctcggtg
     1081 tgacaggagg agcaatcgac tttggccagt ttttccgggt ggggaatttc ttttatgtcg
     1141 tgatggcagg taacgcacgc aaaaacgccg tggactgagc gttcatattt tttctggtcc
     1201 acgaaaagcg ataccgcttg cccggcggga gtatttttgg tcagactgcg gtcgccgtga
     1261 caggtaaagc aatcggcatt ctctatcccc cacgccgtct tggagcagat caggctgaag
     1321 ccgatgagca ctacggagta gtataaattc cgcatttcag gatctcgaat cgctgccttt
     1381 cgctcggccc accggtaact tgtcgtctcg acaccttttg gtcaatggct ggaactgccc
     1441 gtcccgctat cttaacggta cccgaatatc tccgcccaga caatcaagag taccataagc
     1501 aaccccaatg agagcgcgat ccagccgaac atcttcattc cccgcaccac caccggcggc
     1561 aggggctcga ctaaccgctt ctccaattcg ccggaggtca ctaattcctc gtattcgcgg
     1621 ggacgatcca actttaactc ctccaagggc acccgtccgg tgaagatcac cgtgtccatg
     1681 ggaaacttct ccggccggaa atgggtgttg aagaaatgca cggtaaagat gaacccgacc
     1741 gccaaaaggg cctcgtcgga gtgtatgatg gtggccatgt tgatgaaccg gcccgggaag
     1801 atgtgggtga aggtctccgg gaaccagagg aataaaccgg tggcgccgat gatggtcacc
     1861 ccccagaaga cggcgaaata gtcgaacttc tcccagtagg tccagcgccc gtaggccggt
     1921 cggggtccca gaccgacaaa ccacttgaag gttccccaga attccaaaag gtcgatcttg
     1981 ttcggcagca tcgaagccgg accgaagagg acctccttcc aggttttcct ctcccttctc
     2041 ttcttccgaa ccaggtcgaa gatgtgcgtt ccaaaataga aaaaggtgat aaccgcgcag
     2101 acccggtgaa tgtatccggc cgactcaaac ccgcccagca ggcgggataa ccactgcgcc
     2161 catcccaagt aggaaaactt gagcgtcatg ccggtcaaag ccagcccgat gaaactgacg
     2221 atcacgagta cgtgcaggcg gcgggagagg gtgtcgaagc gcaggtactc tttacccggg
     2281 gattggagat ccagtttttt ccgctcgcgc atggcctgaa acgagcgcgg cagccagaga
     2341 atggtgtgca gaccgaagaa ccccagagtt ccgatcaaaa gcaacgtcat ggaccagaaa
     2401 gtgtagaaga gcaccgggta tttgaccggg tcgtggtggg tggcatgggt gaggtacccg
     2461 gcaaagcggc ggtgtgatcc cggatggcac tggccgcagg tcttgacgat gttctgccgc
     2521 gagagccggg acttcgggtc ccaggggggc agaacgtcgt gcgcaccatg gcagtcgtag
     2581 catttggcgg cggcagtgta gcccagtttg gataccttgc cgtggtaggt ctcaaaatac
     2641 ccttccgcca catcctggtg gcagcgcccg cactgggaaa tcattttcag cttaaaccct
     2701 tcctgatcgg tgcgggtgat ggtgtgcgag gtgtggcagt cgtggcagag gggcaggggt
     2761 ttatcagttt tggccttacc gagaaaatgg atgctcagcg cgaattgctc gtagatgccg
     2821 ttgtggcacc gggcgcaggt tttggcgatg ttggcgcggt tcacactgga ggccgaatca
     2881 cgggacggca gctcgtggtg cgccgtgtgg cagtcggtac acatggcagt aaccaccagg
     2941 ccgctctgca acagcccttt accgtggatg ctctcgatgt agttttttac gatcttggtt
     3001 ttctccccgg tcagccgtag ggctgctttc tccccctccc ggtggcattg gccgcacagg
     3061 ttgggcacgt gggtcgggta ggtcggtgaa gtgggatcgc ggcgttcttt ggtgccgtgg
     3121 acgccgtggc agtcccggca caaaggcgca ttgggatcac cccgattggc gagcgtaccg
     3181 tgggtgctcg tggcgtagac cgccgccgcc tcagcatggc aaatcgaaca atcgacccgg
     3241 gtggtgatgg tggcgcaggg ccgcttttcc accggactgg caccggtgtg gcactgggcg
     3301 caggccgtcg tacggtgaat cgagttgcgc atctcgacgg tgtcgataga aagcgaaatc
     3361 gtgtcgccat tgcggatggt aaagagcctg ggctgggcat gacagcggag gcagtcccgg
     3421 tccgacatcc ctaattcgta gaaaactttc cgcacccggt ggggtgcgtg gcagtcgatg
     3481 cagaccggaa ctttattggg ctctttctcc cacagttcgc cccggatcac cttccggtgc
     3541 acctgctcga tcagcccgtg gcacttttca caggttttgg gaacgttttc ccggtaaatc
     3601 gtcgaatgcg gatcggtgtg cgggaggacg ttgtgggcgg tgtgacaatc ggtacagatc
     3661 gccgtcaccg tcagtccttt cttgaacaat ccctccccgt gaacgctgac ggagtagtgg
     3721 gtcaggatgc tgtcctgggg gatgttgtag gttcgggtca ccgcggtgcc ctctttatgg
     3781 caccgcccgc agacgaaagg aacgttgaac cggcgtaccc gggattccgg tgactgcacc
     3841 gggaggatgt cgtgcgcgcc gtggcaatcc cagcagcgcg gggctaactt cgccccggcc
     3901 gctaccgcct ggccgtgcag actgccgcgg tagactgtgg cgacctggtc gtggcagctc
     3961 ccgcaatcga ccggcgcgac ccgttcctgg tgcgggaact ccgcctgcgc cagatcggcg
     4021 tggcagtcaa cgcacgccaa ttgcccatga acggagtttt caaatttctt ctggtcaacg
     4081 tagagggatt ggacgctccc ctcggcggtc gttttcgtta aatccttttc ctgatggcac
     4141 ccgaggcagt ccgcgttggt ggcaaaagaa ctggagggcg agtataagag taaaagtacc
     4201 ccgactcgaa aaacatgttt tagaatgtgc accgactact ccgacatcgc aactctacct
     4261 tatcggtaaa gattcaatca ccggcttggg attttcctct tccgtcgcca aaagactgtg
     4321 gcactggtcg cagtcctggc ggatggtttg gccgtctttg ctctggtggt tgtcgtcgtg
     4381 gcagcggaag cacccggatg aattttggtg cccaatgtta cttctataag tcccccaggt
     4441 aatcttcatc ttggggaaaa cgttgttcgc atagacctgg gctaacttct ccccggcttt
     4501 ttgaatctcc agtgccagac tatcggccag caccggatag ttctgtcgat agtaagtgaa
     4561 aagaccgctc ttgattttct gcaacgcttc cggcttgttc gcatattccg actgcaaaag
     4621 ctccaccgcc gctttcttga catagggcag cccgggatcg atctgccggg tcaacagcgc
     4681 ttcatccacg gcgtcgtccg gcaagcggta cacatgggtg ggccggttgt ggcagtccat
     4741 gcagtccatc acccgctggg gcagtttcgc cagggaatct cgggacacct gaaagtcggg
     4801 agtggcgtat tcgacgattt tgcctttgag gtctttcagg cgcacccagg gaatggtctg
     4861 ccgcgctgca tccgtggcga tgtagctgat ctcgttggcg atgttcatgt gccagtggat
     4921 cccctccgca atgccggatt ctttgtctcc gcccccggtc ttaagcagga gcacggtttt
     4981 caaggccgta ttcttctcgt ctgcggaata gtgggtgaac acccgcagtt gctgcccgtg
     5041 gaacttttgg ggccagtggc actgctcgca ggtttcccgc gccgggcgca ggttcttcac
     5101 cggcgtggga atggggcggt cgtaggtgtt gaaggtcacc gcgaacacct gccccacgcc
     5161 ggaaagtttg ctcttgacga accagggcgc gcccgggccg atgtggcagc tgacgcaggc
     5221 gacccgggag tgcggggaat gcgaataggt gacgagctcc ggctccatca ccttgtggca
     5281 cacctgcccg cagaaggtca ccgactccat gaattccacc gccttgtagg tggccgtggc
     5341 caggatcgaa aggaacaagg tggagaggac cgcgagccag gcaattcgtt tgcgcaagac
     5401 cggccggttt aaatcgagtt cgattttcgg caaggccagc tctttgggca ggccttgctg
     5461 gagatgcacc cgccttttct ccaaagccaa ccccaccggg accatgacca aaccggccac
     5521 caggaacgcc ggcaggatga gatagactaa aatgccgatg tagggactga tctggacgcc
     5581 caaaaggtca atcgccacga tggtgataac cagaaccgaa gagactaaag cgaaaagcaa
     5641 accgagaaag gagaggagat tgcgcgagac gcgggcgaaa agtttcttca ttgcgtccgg
     5701 cccgctttct ctttctgccg ggcttcgatc cggcggattt tcaaccacag cccgatgcac
     5761 agaagcaaaa tcaccaccgc cgaaaaagcc aatcctttgc ggcggatctc caggtcttcc
     5821 agcgacgcca gagccgcttt tttcacctgc agggcccggc ccattccgtc acgggtgatc
     5881 gcctgaaaat cggccagaga aaaagagtgc agggctgagc gggagcgaat gacggcatct
     5941 tcggacgcct tcaagtcgaa tttggccagg gcggttttga cccccgccac ttcggcccgc
     6001 tccagaagcc ctcgggcttc ggccaccgcg ctcttgagcg aatcaaggct cgccttgaac
     6061 tcttccgccg aactgtagcc cttatccccc tcgctgtgac agcggatgca aaccgcttcc
     6121 ggccccgtac cgaggaatgc atcagttggc cgcagcactt tatgattatc gtggcaggtg
     6181 gcgcactgcg gcaggccggt ccgggcaaag ggaattttgt gcggcgattt ttcaaaaaga
     6241 tcccggttgg ccggatggca ctcgccgcag gccccggcca cgtcggaaag ccctggcggt
     6301 gtggccccgt ggttgccgtg gcaatcggta caggccgccg ccgaccgatc gcctttatcc
     6361 aagagcaatt ctccgtgtac gctctgacgg tagtcgtcat actggttggt gggaatgccg
     6421 taggatgcca tccgcttggt gtcggcgtga cagtgtgcgc aggtaccggg aatgtgcgtt
     6481 ttgaacaccg gtgagcgggt gtctgagggt ggcaaaatcc cgtgcacccc gtggcaggaa
     6541 acgcagttgg ccacgagggt atcccctctg actaaccgct ttccatgcac ggaggttttg
     6601 aactgagcat actggtcggt gggtagccgg gggtcgtacc gcttcatata ctcgacgtcc
     6661 gaatgacagc ggccacagaa ggcggggatt tgtgccggct tgggcgcgcc gacgtaccc
//
LOCUS       DBNGZD010000258         4052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_597659, whole genome shotgun sequence.
ACCESSION   DBNGZD010000258 DBNGZD010000000
VERSION     DBNGZD010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_597659"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16GM_06805"
     CDS             complement(<1..461)
                     /locus_tag="AC16GM_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR2923420.1"
                     /translation="MPKRKNRPHPPVAVATCAVHAGEEERKYARAISFPIFQTTNFQF
                     RTMREFYRYLKGEPDQYLYSRYTNPTIEAAEKKMAALEGAEKALLFSSGMAAISGAIL
                     TLAQTGQELVATRTLYGGTFQLLTEILPRLGIRTRFVDTQDLEQAEKVIGR"
     gene            complement(454..1404)
                     /locus_tag="AC16GM_06810"
     CDS             complement(454..1404)
                     /locus_tag="AC16GM_06810"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR2923421.1"
                     /translation="MGKTAIFYHPDFLLHNAGQDHPERPDRLLAIVDRLKQEPFAGKL
                     EWVEPGSAFPTWITTVHAETYRAQIEALSRAGGFRLDPDTAGGPDTYRAAMRAAGAVL
                     TAVDRVMKKENPTAFCLVRPPGHHALPDRGMGFCIFNNVAIGVRYLQEKYGLKRVTII
                     DWDAHHGNGTQAIFYRDPTVFYCSLHQYPFYPGTGKTDEVGAGPGKGFTVNCTLPAGS
                     GDQEFLAAFNDIFLPRLKDFAPEFIFLSAGFDAHRADPLTGLQVTEAGFREASRLVRA
                     LAQECCQGRLVSVLEGGYNLAALARSVAVHIEALLDEEHA"
     gene            1607..2473
                     /locus_tag="AC16GM_06815"
     CDS             1607..2473
                     /locus_tag="AC16GM_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR2923422.1"
                     /translation="MHPRTRADLILMSITVLWGSTFAVVKDTLTLVPPFFFLTLRFGL
                     AFLAVLLIFPNRLKDWDWPTVRAGIILGLLLFIGFAFQTIGLAEIPATRSAFLTGLVV
                     IFVPFIAYFRLKERMTLNLVAGALLALLGLYLLNRPHGWTVRWGDLATILCAWFFAWQ
                     LVYINIYTPRFAIPHLITVQFGVSTVAGLVLTVLFEKPPLAYPPKLWGSVLLMGLLAT
                     ALAFYLQNRFQKDTTTVRAAIIYNLEQIFAVLFAFLLLGEVLEVSEYLGGAAIIGGVL
                     VAEVGKGRSTVF"
     gene            2566..>4052
                     /locus_tag="AC16GM_06820"
     CDS             2566..>4052
                     /locus_tag="AC16GM_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923423.1"
                     /translation="MLRVARLRLLLLAGIFVLAPLVASAAKKEKKEKKQEPLVLENAD
                     AYLRVDSAGMVIHHLDGHVLFKHGDARLQSDHADWYETQKRIEFSGRVVFNYPDQQVK
                     SEHVTYYIPDKRALGTGGVELIDLKEDAVVTGGRGDYNRNEKHLRVTENPVLVLKAKD
                     TLQTVTVTSHFMEYFAEEKRGLATDSVEIKKKEMHARCGRAEFLSRDDHMILTDKPFA
                     FDSRSTLAGERMDFWIKNEKIEHIDVTGNARGTYCEKADSADTACAQSVLSAKAVSFY
                     FQDEKLVKVLATANAYSFYTPAPGDTANTGKNEASGDTITLFLEHEQVEQVTVTGGAQ
                     GTYVAPRREGGRLTGEDTVNYAAHFIDYHINEDEIALVGSGSVQYQNLTLSADSIHYH
                     TETQILNAGGLALTPPGEKRYRLQDGSDEIFFSRLSYNIRTRKGRLKQSSTQLEGGYY
                     HGSNLAKVDENVLLVRQGRYTTCDDPNPHYYFGSRNMKIVAKDKVV"
BASE COUNT          871 a         1170 c         1165 g          846 t
ORIGIN      
        1 ttgcggccta tgactttttc ggcctgctca agatcttgag tatcgacgaa tctggtccgg
       61 atccccaagc gcgggaggat ctccgttaac aattgaaacg tgccgccgta gagggtgcgg
      121 gtggcgacaa gttcctgtcc ggtttgggcc aaggtcagaa ttgctcccga aatggcggcc
      181 attccggagg agaagaggag ggccttttcg gcgccttcca gcgccgccat tttcttttcg
      241 gcggcttcga tggtcgggtt ggtgtaccga ctgtagagat attgatccgg ttcgcctttc
      301 agatatcgat agaactcccg cattgtccgg aactggaagt tggtcgtctg gaaaatgggg
      361 aaggagatgg cgcgggcata ttttctctct tcctcaccgg cgtggacggc gcaggtggcc
      421 acggctactg gcgggtgcgg acgatttttg cgtttaggca tgctcttcgt ccagaagtgc
      481 ctcgatatgg acggccaccg accgggccag tgcggccaga ttgtagccgc cttccaggac
      541 cgaaaccaac cggccctgac aacattcctg cgccagagcg cgcacgagac ggctcgcttc
      601 ccgaaacccc gcttcggtca cctgtaaccc ggtcagggga tcagcccggt gcgcgtcaaa
      661 cccggcggag aggaaaatga actccggggc aaaatctttc aagcgcggta aaaaaatgtc
      721 attgaaagcc gccagaaatt cttggtcgcc ggaaccggcc gggagggtgc agttcaccgt
      781 aaaccctttc cccgggccgg caccgacctc atcggttttc ccggtgccgg gatagaacgg
      841 gtactgatgg aggctgcagt aaaagacggt cggatcccga tagaaaatcg cctgggtgcc
      901 gttgccgtga tgggcgtccc aatcgattat cgtcactcgt ttcaagccat atttctcctg
      961 caggtagcgt acgccgatcg ccacgttgtt aaagatgcaa aaacccatac cccggtcggg
     1021 cagcgcatga tgcccggggg gccggacgag acagaaggcg gtgggattct cttttttcat
     1081 aactctatca acggcggtca ggaccgcacc cgcggcccgc atggccgccc gataggtgtc
     1141 cggccctccg gcggtgtcgg gatcgagccg gaagccgccc gctcgcgaaa gcgcctcgat
     1201 ctgcgcccgg taggtttcgg catggacggt ggtgatccag gtcggaaacg ccgagccggg
     1261 ttcgacccat tccagcttcc cggcaaaggg ctcctgcttc aggcggtcaa ctatcgccag
     1321 caggcggtcc ggccgttcgg gatggtcttg accagcattg tggagcagga aatcgggatg
     1381 gtaaaagatc gctgtcttgc ccattaggac cgctttgcgt cacctggttg cgtcgcaatt
     1441 tagttcatca acgctttaac gcaagtaacg agagagcggg gatactccgg ccacgcgcag
     1501 aaaaatttct tgcccgctgg gaaattttac cgtatggtat tgagccggat gttcgctcaa
     1561 gcccgtcccg ctgcaagcaa ccgaccactc ctgttgcccg ccaacgatgc acccccgcac
     1621 ccgcgctgat ctcatcctga tgagcatcac ggtgctctgg gggagtactt ttgcagtggt
     1681 caaagacacc ctgactttgg tcccgccttt tttcttttta accttgcggt ttggtctggc
     1741 atttctggcg gtgcttttga ttttccccaa ccgcctgaaa gattgggact ggccgacggt
     1801 cagagccggg atcattctcg gcctcctgct cttcatcggg tttgccttcc aaaccattgg
     1861 cctggccgaa attccggcca cccgctccgc ttttctgacc ggtctggtcg tgatcttcgt
     1921 gccgttcatc gcctatttcc gactgaaaga acggatgacg ctcaatctcg tggccggggc
     1981 gctcctggcg cttttgggac tctacctttt gaaccggccc cacggctgga ccgtccgctg
     2041 gggggatctg gccaccatcc tctgcgcctg gtttttcgcc tggcagctgg tctatatcaa
     2101 catttacacc ccgcgtttcg ccattcccca cctgattacc gtacaatttg gggtgagcac
     2161 ggttgccgga ctggtcttga cagttctctt tgaaaagccg ccgctggcgt atcctcccaa
     2221 actgtggggg agcgttctcc tcatggggct tttggccacg gccctggcgt tctacctcca
     2281 gaatcgcttt caaaaagata cgactacggt gcgggcagcc attatctata acttagagca
     2341 gatttttgcg gtgctgttcg ctttccttct gcttggggaa gtgctggaag tgtccgaata
     2401 cctgggggga gcggcgatca ttggtggagt gctggtagcg gaggtgggca aagggcggtc
     2461 aacagttttc taacggatga aaacgaattc atcaaacgca ctccatttcc gtaaagaaac
     2521 ctgatagtgg aagagagcag aaaacaaacc gcctttcggc cgattatgct aagagtagcc
     2581 cgattacgct tgctgttgct ggctgggatc ttcgtcctgg ctccgttagt cgccagcgca
     2641 gcgaaaaaag aaaaaaagga aaagaagcag gaaccactgg tgctggaaaa cgccgatgcc
     2701 tacctgcggg tcgattcggc cggaatggtg atccaccacc tggacggcca cgtcctcttc
     2761 aagcacggcg acgcccggct gcagagcgat cacgccgact ggtacgaaac ccagaaaaga
     2821 attgaattct ccggtcgggt ggttttcaac tacccggatc aacaggtgaa atcagagcac
     2881 gtcacctact atatcccgga caagcgggcc cttggcactg gcggggtgga gttgattgat
     2941 ctcaaggaag acgccgtggt cacaggaggc cggggagact acaaccgaaa cgaaaagcat
     3001 ctgcgcgtca ccgaaaaccc ggttttggtg ctcaaggcca aagacacgtt acagaccgta
     3061 acggtgacgt cacacttcat ggaatacttc gccgaggaaa agcgcggcct ggccaccgac
     3121 agcgtggaga tcaaaaaaaa ggaaatgcac gcccgctgcg gtcgagcgga gttcctgagc
     3181 cgcgacgacc atatgattct gaccgacaaa cccttcgcct tcgattcccg gagcacgctg
     3241 gccggtgagc ggatggactt ctggatcaaa aacgaaaaaa tcgagcacat cgacgtcacc
     3301 ggtaatgccc ggggcaccta ctgtgaaaag gccgattccg ccgatacggc ctgcgcgcag
     3361 agcgtgctct ccgccaaagc ggtctccttc tacttccaag acgaaaaact ggtcaaagtg
     3421 ctggcaacgg ccaatgccta ttccttctat acccccgcgc cgggggatac ggccaacacc
     3481 ggcaagaacg aagcctccgg cgataccatt actctctttt tggaacatga acaggtcgag
     3541 caggtcacgg tcaccggcgg ggcgcaagga acctacgtgg ctccccggcg ggaagggggg
     3601 cgcctgaccg gcgaagatac ggtcaactac gccgctcatt tcatcgacta ccatatcaac
     3661 gaggatgaaa tcgctttagt cggctccggg agcgtgcagt atcagaacct cacgctcagc
     3721 gccgattcga ttcactatca caccgaaacg caaattctca acgccggcgg gctggcgctg
     3781 acgccgccgg gagaaaagcg ctaccggctt caggacggct ccgatgagat tttcttttcg
     3841 cgcctgtcgt acaatatccg cacccgcaaa ggccggctca agcagtcgtc cacgcaactg
     3901 gaaggcggct actaccacgg ctcgaactta gccaaagtcg acgagaacgt tctcctggtg
     3961 cggcaggggc ggtacaccac ctgcgacgac ccgaatccgc actactactt cgggagccgg
     4021 aatatgaaaa tcgtggccaa agataaagtt gt
//
LOCUS       DBNGZD010000259        14397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1221828, whole genome shotgun sequence.
ACCESSION   DBNGZD010000259 DBNGZD010000000
VERSION     DBNGZD010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14397
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1221828"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="AC16GM_06825"
     CDS             complement(<1..511)
                     /locus_tag="AC16GM_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424898.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR2923424.1"
                     /translation="MVILIILDGFGLNPKREANAIALAKKPNLDRLFATYPTTQLEAS
                     GPAVGLPPGQMGNSEVGHLNLGAGRIVYQEITRIDRAIADGSFFENRALVGAMERCRR
                     NKSRLHLMGLVSDGGIHSSQEHLYALLDLARRKGAGEVYIHAFLDGRATMPPTGAGYL
                     KQLLEKCRSL"
     gene            complement(600..1901)
                     /locus_tag="AC16GM_06830"
     CDS             complement(600..1901)
                     /locus_tag="AC16GM_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2923425.1"
                     /translation="MASGSKPVVLVVGRLSGQKNRVIVNTLKFAAPEVVKVFPQAQFQ
                     VIGSPVQAEHRHLEKQHGFIRFYGHQPDLRSFYEDATIVVGAGRVALEAMALKKAVVA
                     IGERLYVGPILAEVIEQAKATNFGDCFEKEVFDWPRMSQDIVRLLHNDRLREQVALTG
                     YELAQSEYDLQKIYLQIEAVYQQAVLKNNLARKYEIPVLQYHRVVAEIPGKSRFNIYV
                     TRADFEKQLAVLKRHSFTPITFSDFLSRRLPQKPIILTFDDGYADHYHIAFPLLKKYG
                     LKAVIYILGNRNITSNCWDVPLGEPEVPLLKPEQIREMAESGLVDFGGHSMDHRNLIA
                     LSTTEMEREIVDSKKSIEEMIGQPVASFAYPYGSYNGKVKECTAAAGYIFGVASDRGP
                     ARFGADLFAIRRIPIFPHTSYWQFRKKTSGYYLRYRTLLGK"
     gene            complement(1885..2361)
                     /locus_tag="AC16GM_06835"
     CDS             complement(1885..2361)
                     /locus_tag="AC16GM_06835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2923426.1"
                     /translation="MNILHVLSGDFVSGAETYAAALIDEQMRRGHNVFIASGSFSQPT
                     GARFIVLPIYQRNYIRRVRNVLTLLRLIRREGIDIIHAHSRAACWVSHWAAKFTNTAF
                     VTTLHGRLHRRRSLLAYNVYGNTCIAVCEKIRDQLLSETASLRPEQVVVIRNGIRL"
     gene            complement(2508..3899)
                     /locus_tag="AC16GM_06840"
     CDS             complement(2508..3899)
                     /locus_tag="AC16GM_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR2923427.1"
                     /translation="MRHVPFGQLFINKLVIVALGLLLGVALAQAGQDRVLKLFGDLQN
                     VIISVSDRIKPSVVHIEVVQKFDNNKFETLGSGLIVDERGYILTNEHVVDNAQSVTVT
                     LPSKREYPAEVVGVDKQTDLALLKIDAPEKLTAAPLGNSDDVQVGEWVIAVGNPYGFD
                     RTVSFGVVSGKGRVLPRLSMDMPLINNFIQTDAAIDPGSSGGPLVNLQGEVVGINSMG
                     VGRGQGFTIPINIAREVKNKILTSGVIERGWIGIVMQPFPRDFARYFSQPDLEGILVS
                     DVLENSPAAGAGLKPGDVITRFNGREVSAEKDEDLNSFSLTVTQTNIGDRIPVSVSRA
                     GKPVELSITIGTQPKVKAEEFETGLGFTVKEITESIFLQSGLADKDGVLVSYVEVGGE
                     ASKAQLGEGDVVHSVEGETVADLAGFKATLAKVKEKPQILLTVKRGKNNRYIILNHSP
                     ARGVGQTPELPQN"
     gene            4183..5691
                     /locus_tag="AC16GM_06845"
     CDS             4183..5691
                     /locus_tag="AC16GM_06845"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR2923428.1"
                     /translation="MIKKFFTRENLPWVVFLILVSVAVGIILASGLDLPSRTLAGYPA
                     QAAGQEKFSGHSPFVAVAARVKDAVVNISAEKVVGRRPSFFDQFFFYQHPGRAKYLSL
                     GSGFIFDQRGYILTNNHVISGADNIQVKLSDESVYEAKIVGADPATDIAVLKIKASRD
                     LPSVVLGNSDSIFVGDWAIAVGNPFPQQGLDRTVTVGVISAKGRSNLVFGDQQPVYQD
                     YIQTDAAINHGNSGGPLVNISGEVIGINSAIATSSPDGGNVGVGFAIPINLAKAVLPS
                     LMKAGKVERGWLGLQPRDLTPDLAEALDLPVKEGVLVEQVVQNTPAAQSGLKAGDVIL
                     SFAGKKVSSAQEFRLLVAATSPEKTVTLDILRNGRKKTLEITLAKRPEQMTELPQGEE
                     NNGAEAPVRGTTRRWLGMTVTTATRDLCDSYDVAYYPGLMVTEVETGSPADNQGIEPG
                     FIITEVAGEKVAAVGDFLQVTEKLKRRSRPILIAVRDREAVAHYVALRPVSG"
     gene            5726..6580
                     /locus_tag="AC16GM_06850"
     CDS             5726..6580
                     /locus_tag="AC16GM_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR2923429.1"
                     /translation="MARASKKYRDEDRSLDVYLREIGETPLITAQDEIELAKRIRVGD
                     QSALEKLTKANLRFVVSVAKQYQNQGLSLADLINEGNIGLIKAAKRFDETRGFKFISY
                     AVWWIRQAILQALAEQSRIVRLPLNRVGTLHKIGKISSALEQEFGREPSHEEIAKELE
                     LSSVEVSDTLKISNTHLSLDAPFTQSEDNSLIDVLEDAEQPPPDEQLLTDALKMEIGK
                     ALRSLTPREAEVIKLYFGLHHEKALTLEEIGARFSLTRERVRQIKEKAIRRLRHASRS
                     KALRAYLN"
     gene            7691..9262
                     /locus_tag="AC16GM_06855"
     CDS             7691..9262
                     /locus_tag="AC16GM_06855"
                     /inference="COORDINATES: protein motif:HMM:NF049774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7619 domain-containing protein"
                     /protein_id="HIR2923430.1"
                     /translation="MSGRDWFTIRGQRSVCILLIGGVLCCTGTTDDVYAGPFVDSVIV
                     SSKIFASGSQDVIEVKIHNDSTLNGWTIPLVIRSQTGGAFGRVDSVISVAPFGGARVL
                     KVNGVTPDSLVITGVSLGVAPGPRRTAFRLFVTNNSQFGTYSLDSNRLSASQALKLTH
                     PPAQPIVPVFVRGLESIATGNFPDLHATVGGSRARPGFSKLYGISYGNIGSLTTIGTV
                     TLALPRQVIHQSSSDGGVYNPGTHSVTWNVGPLAPGFIDWLVTVVGIPNSVPLGTTLI
                     SSVYIEALGGDPNPGNNTDWESQFVRGAFDPNEKLVSPQGEGPFGLISRTDTLRYQVN
                     FQNVGTDTAFDIVVRDTLDNNLDEATVESGASSHPYVFSIAGRELSWTFTNINLPDSI
                     VDELGSHGFVTYKVRPKANATSGTDIKNRAAVYFDFNPPVITNTVSNRICDRRGDVAG
                     DPNTNCPDRTIDIVDIVAVINNVVFNTPFCGPADVADVAGDPNGCPDTNVDIVDIVSI
                     INNVVFGTPFCLECL"
     gene            9628..10701
                     /locus_tag="AC16GM_06860"
     CDS             9628..10701
                     /locus_tag="AC16GM_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923431.1"
                     /translation="MMTRCVRFAVLGMVGTGFAWASAVFAVPPPNDNCSNAISIREVA
                     NIPFSTEGATFDGPGGCMTSPNIWYRYTASCTGPVTVSLCGSNYDTKLAVYSGSCGSF
                     GAPLFCNDNFCGLQSRGTFTAIAGNTYLVEVGGAGSATGLGVLSVCCGPPPSNDNCAN
                     ATPIGEVTDLPYSTLCASFDGPGGCITSPNVWFRYTASCDKAVSVSLCGSDFDTFLGV
                     FDGGGCNPVGPPVLCQDDYCGLSSHGTFRAQGGHTYLIEIGGYVGTTGTGTLTISCCV
                     PRGDVAGMAVTNCPDGVVGIVDVIAVINKVVWGIPYCGPVIAGDVGGAPDTNCPDGVV
                     NIADILTLIKYSVFAIPLCEECL"
     gene            10820..10893
                     /locus_tag="AC16GM_06865"
     tRNA            10820..10893
                     /locus_tag="AC16GM_06865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10854..10856,aa:Arg,seq:tcg)
     gene            10927..11655
                     /locus_tag="AC16GM_06870"
     CDS             10927..11655
                     /locus_tag="AC16GM_06870"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR2923432.1"
                     /translation="MGNRLLANLGTLGHAILQFIYPLECSLCEQPVEEHNAPLCEKCF
                     ASLATVDQPFCLFCHKFLGPTVACVSCDSGLPQVPTLSLGAFDDSYQKLIHQLKYRNA
                     RPLGKLFGQRMAECLGQLEHFGPIDVVIPVPLHRRRKWKRGFNQSDLVAEGIAKALGK
                     PLDKRTLRRLRHTQDHTRLSRVERQRNVAGAFGVVNPDRIQGKTVLLVDDVTTTGATL
                     GECARTLVNAGAKRVCAAAIAVAL"
     gene            11746..12984
                     /locus_tag="AC16GM_06875"
     CDS             11746..12984
                     /locus_tag="AC16GM_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HIR2923433.1"
                     /translation="MVDTNLFNRITARIDASKPAMIDLQKKLSAIPAIGPKNDGKGEW
                     EKSRFLKSYLEQNGFAQIQEYDAPDPSVPNGVRPNFVVKVPGRSSSKTVWIMTHMDVV
                     PPGERTLWENDPFTVIEKDGKLIGRGVEDNQQGLVSSLFAVKAFLDEKITPAFDTGLI
                     FVSDEETGSELGIGYLLKNFQLFKPEDLVIVPDAGNEKGTLLEVAEKSICWLKFTTKG
                     KQCHASMPHKGVNSLRAASHLIIRLGELYRLYPIKDEMFDPPLSTFEPTKKEANVPNV
                     NTIPGDDVFYLDCRILPQYKVVDVVKTIRRICDEVEQEFKVTIAIASQQGEQAAPPTP
                     ANAPVVQALKRAIKDVYKVDAVPMGIGGGTVAALFRRAGFPAAVWAKMDEMAHQPNEY
                     CWIDNIVGDAKVFAHVLLQG"
     gene            13581..14045
                     /locus_tag="AC16GM_06880"
     CDS             13581..14045
                     /locus_tag="AC16GM_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR2923434.1"
                     /translation="MRKTFVIAVLVFAAGSIALGQKQSARRNSKGSVEQAIRQVDHER
                     IQAQIGADAVALDRIYADDFIGIGPSGTVRTKAQVIADFTSGELKFQSITTDDVQLRV
                     YGNTVVETGRSTMIGQDKGKAVPRDNRFTRVWIKQGRRWRLVANHYSLLIAQ"
BASE COUNT         3232 a         3891 c         4111 g         3163 t
ORIGIN      
        1 ccagagagcg acatttttcc agcaactgct tcaggtagcc cgcacccgtg ggcggcatcg
       61 tggcccggcc gtccagaaaa gcgtgaatgt agacttcacc ggctcccttg cgccgcgcca
      121 aatctaaaag cgcgtacagg tgctcctggg aactgtggat gccgccgtcg gagaccaatc
      181 ccataaggtg cagacgggat ttattccggc ggcagcgctc catcgctccc acgagagcac
      241 gattctcaaa gaaactgcca tccgcgatgg cccggtcgat gcgggtgatt tcctggtaga
      301 caatccgccc cgcacccaag ttcaaatgcc ccacttcact gttccccatc tgtccgggcg
      361 gcaaacccac cgccgggccg gaggcctcca gctgcgtggt ggggtaggtg gcgaaaaggc
      421 ggtctaagtt cggcttcttc gctaaggcaa tcgcgttggc ttctcttttg ggattgagcc
      481 cgaagccgtc gaggataatg agaatgacca taccgctaca agtctgacgg caaatttaac
      541 tccgggtgac tctttcgcca aataccattt ccacagtccc ggggttaacg gagaaaacgt
      601 catttgccca aaagcgtccg gtaacgcaaa tagtagccgc tggttttttt acggaattgc
      661 caataagaag tatgggggaa aatcgggatc cggcggatgg cgaataagtc ggctccgaag
      721 cgggcgggac cccgatcgga agcgacgccg aagatatagc cggctgccgc ggtgcattcc
      781 tttacctttc cattataact cccataggga taggcgaacg aggccacggg ttgtcctatc
      841 atctcctcga tggatttttt gctgtcaact atttctcttt ccatttcggt cgtcgagaga
      901 gcgattaagt ttcggtggtc catcgaatga ccgccgaaat ccaccaggcc actttcggcc
      961 atttcccgga tttgttccgg tttcaggagc gggacttccg gttcgcccag cggaacgtcc
     1021 cagcaattag aggtgatgtt tcgattgccg aggatgtaga tcaccgcctt cagaccatat
     1081 tttttcagca gggggaaggc gatgtgataa tgatctgcat acccatcatc aaaggtcaag
     1141 atgatgggct tttggggaag tctccgggat agaaaatcag agaaggtgat aggggtaaag
     1201 ctgtgtcgct tcaaaactgc aagttgtttt tcaaaatcgg ctctggtaac atagatgttg
     1261 aacctgcttt ttccgggtat ctcagcgacc acacgatggt actgtaaaac tgggatttcg
     1321 tattttcggg cgaggttgtt tttgagaacc gcttgctggt agaccgcttc gatctgcagg
     1381 tagatttttt ggaggtcgta ttcggactgc gccagttcgt acccggtcag cgccacctgc
     1441 tcgcgcagac ggtcattgtg cagcagccgc acaatatctt gggacattct gggccaatcg
     1501 aaaacctctt tttcaaaaca atcgccgaag ttcgttgcct tcgcttgttc gattacctcc
     1561 gccagaatgg gccccacgta cagccgttcg ccaatggcca ccaccgcttt tttaagcgcc
     1621 atcgcttcta gcgccacccg gcccgcaccg accacgatgg tagcgtcttc ataaaaagac
     1681 cggaggtcgg gttgatgacc atagaaacga atgaacccgt gctgcttttc taagtgccgg
     1741 tgctctgcct gaaccggcga gccaatgacc tggaactgcg cctgggggaa aaccttcaca
     1801 acctcaggcg ccgcgaattt cagggtgttg acgatgaccc tgtttttctg gcccgacaac
     1861 cgcccgacga cgagcaccac cggtttagag ccggatgcca ttgcggatca ccaccacctg
     1921 ttcagggcgg agcgacgcgg tttcggagag cagttgatcg cggatcttct cacagactgc
     1981 aatgcaggta ttgccataca cattgtaagc caaaagcgaa cggcgccggt gcagccgccc
     2041 gtgcagggtg gtaacgaagg ccgtattggt gaatttggct gcccagtgac tcacccagca
     2101 ggcggcgcgg gagtgggcgt gaatgatgtc gatcccttcc cgacggataa gccgcaaaag
     2161 cgtcaacacg tttcggactc gccggatata gtttcgttgg taaatgggga ggacgatgaa
     2221 tcttgccccg gtgggctggg aaaaacttcc ggaggcgata aagacgttat gtcctctgcg
     2281 catttgctcg tcaatgagcg cggcggcgta ggtttcggct ccggaaacga agtcgccgct
     2341 taagacgtgc aggatgttca ccggttttct ctattcagca gtggagaacc cgaagggctt
     2401 ggggctgcaa aaaaatagcc acgcacggtt taaggacttc acctctcacc ggcgacgagg
     2461 cataggagag aactccctgc gacatgcaga gggaataaaa agcgaactca gttctggggc
     2521 aactcgggcg tctggcccac gcctctggcg ggggagtgat tgaggatgat gtagcggttg
     2581 ttcttgccgc gtttgaccgt gagaaggatc tgcggtttct ctttgacttt ggctagggtc
     2641 gccttgaacc cggctaagtc ggccaccgtc tccccttcaa ccgaatgcac cacgtcccct
     2701 tcgcctaact gcgccttgct cgcctcgccc cccacttcga cgtaggaaac cagaacgccg
     2761 tctttatccg ccaggccgct ctgcaggaaa atcgattcgg tgatctcttt gaccgtgaat
     2821 cccagcccgg tttcgaactc ttcggctttc accttcggct gggtgccgat ggtgatggag
     2881 agctccaccg gctttcccgc gcgggagaca gataccggaa ttcgatcgcc gatgttggtc
     2941 tgggtcacgg taagcgaaaa actatttaaa tcctcatctt tttccgcgga gacttcccgc
     3001 ccgttgaagc gggtgatgac atcccccggt ttgaggccgg ctcctgcggc gggggaattt
     3061 tccaaaacat cggagaccag aatcccctct aagtcgggtt ggctgaagta ccgggcgaaa
     3121 tcgcgcggga acggctgcat gacgatcccg atccagccgc gctcaatcac tccggaagtc
     3181 aggattttgt ttttcacttc tctggcgatg ttaatcggaa tggtgaatcc ctggccgcgc
     3241 ccgaccccca tggaattgat cccgaccact tctccctgca ggttgaccag aggcccgccg
     3301 gaggacccgg ggtcgatcgc cgcatcagtc tggatgaagt tattgatgag cggcatatcc
     3361 attgacagtc gggggagcac ccggcctttt ccagacacga ctccgaacga caccgtgcgg
     3421 tcaaagccgt acggattgcc cacggcaatg acccactccc cgacctggac gtcgtcggag
     3481 ttgcccagcg gtgcggcggt caatttttcc ggcgcgtcga ttttcagcag ggccaggtcg
     3541 gtctgcttgt ccacccccac cacctcggcc gggtactcgc gtttggaggg caaagtgacc
     3601 gtcactgact gggcgttgtc caccacgtgc tcattggtga gaatgtagcc gcgctcgtcg
     3661 acgattaagc cggaacccag agtctcaaac ttgttgttgt cgaatttctg gacgacttcg
     3721 atatgaacca ccgaaggctt gatgcgatcc gagacggaaa ttatgacatt ttgcagatca
     3781 ccgaagagtt tcaagacccg atcctggccg gcctgcgcca gagccacccc caggagtaat
     3841 cctaaagcga cgataactaa tttattgata aatagttgtc caaaggggac gtgtctcatc
     3901 tgccttctca gcttcgtcat atccttcttc tcggcaaatt atacgaaact ttttatcaat
     3961 caaggagaaa aagcccgcaa caaaaaaggt gcaacggcgt ataattatat tgaagctgtt
     4021 ttgttgtaag taagttgttg tacagtcgta acttatcgga agctggtctg aagactgacc
     4081 aaaagaacga aaaccacatt ttaggtctgg cgcaaaaatt gtccttgaca aaaggggata
     4141 aagggttaag tttatttttc ttttggggga gaaaaaacga gcgtgattaa gaaatttttc
     4201 acaagggaaa atcttccctg ggttgtcttt ttgattctgg tctcggtggc cgtggggatc
     4261 atcctcgcct ccggtctcga tctcccttcg cgcactctgg ccgggtaccc ggcccaggcc
     4321 gccggtcagg aaaagttctc cggccattcc ccatttgtgg cggtggccgc ccgggtcaaa
     4381 gatgcggtgg tgaacatttc cgccgaaaaa gtggtgggcc gcaggccgag tttcttcgac
     4441 cagttctttt tctaccagca ccccggccgg gcgaaatatc tgagtctcgg ttcgggcttc
     4501 atcttcgacc agcgtgggta catcctgacc aataaccacg tcatttcggg cgcggataac
     4561 atccaggtga aattgtccga cgagtcggtc tacgaggcca aaatcgtggg cgccgacccg
     4621 gccaccgaca tcgccgtttt gaaaatcaaa gccagccggg atctcccctc ggtggttttg
     4681 ggtaattcgg actcgatttt cgtcggcgac tgggccattg ccgtcggcaa tcctttcccc
     4741 cagcaggggc tcgaccggac cgtcactgtc ggggtgattt ccgcaaaggg ccgctcgaac
     4801 ttggtcttcg gcgaccagca gccggtctac caggattaca tccagaccga tgccgccatc
     4861 aatcacggca attcgggcgg gccgttggtc aatatctcag gcgaagtaat cggcattaac
     4921 tccgccattg ccacttcgtc tccggacggc ggcaacgtgg gcgtcggctt tgcgattccg
     4981 attaacttag ccaaagcggt gctcccttcg ttgatgaaag cgggcaaggt cgaacggggc
     5041 tggctgggcc ttcagccgcg cgatttgacg cccgatttgg ccgaagcgct ggatctgccg
     5101 gtcaaagagg gcgtgctggt ggagcaggtt gtgcagaaca cgccggcagc gcagagcggc
     5161 ctcaaggcgg gcgacgtaat tctctccttc gcggggaaga aggtctccag tgcacaggaa
     5221 ttccgccttt tggtcgccgc tacttctccg gagaaaaccg ttacgctcga catcctccgc
     5281 aacggccgca agaagacttt ggagattacc ctggccaagc ggcccgagca aatgacagag
     5341 ctgccgcaag gagaagagaa taatggcgcg gaagcgcccg tacggggaac cacccgccgc
     5401 tggctgggga tgacggtgac caccgccacc cgagacctgt gtgacagtta cgacgtggct
     5461 tactatccgg gcttgatggt cacggaagtc gaaaccggca gtccggccga caaccaggga
     5521 attgagcctg gcttcatcat caccgaagtg gcgggagaaa aagtggcggc ggtgggagat
     5581 ttcttgcaag tcacagaaaa gctgaagcgc cggtcgcggc cgattttgat tgcggtccgg
     5641 gatcgggaag cggtggcgca ctacgtggcg ctgcggcctg tttccggata attgagggag
     5701 cccccaaggc aaggagccaa aacgcgtggc acgagcatcg aagaaatacc gggacgaaga
     5761 ccgttcgttg gacgtctacc tgcgggaaat cggcgaaacg cctttgatta ccgcgcagga
     5821 tgaaatcgaa ctggccaagc gaatccgggt gggtgaccag tccgccttag agaaactgac
     5881 caaggccaat ttgcgcttcg tcgtttcggt ggccaagcag tatcagaacc aggggctatc
     5941 gctggccgat ctgatcaacg aaggcaacat cggcctgatc aaagccgcca agcgttttga
     6001 cgagacccgc ggcttcaagt tcatctccta tgcggtctgg tggatccggc aggcgattct
     6061 gcaggccttg gccgagcaat cgcggattgt gcgcttgccc ttgaaccggg tcggcaccct
     6121 gcacaagatc gggaaaattt ccagcgcttt ggagcaggag ttcggccgcg agccctcgca
     6181 cgaagaaatc gccaaggaat tggaactctc gagcgtcgag gtgtccgata cgctcaagat
     6241 ttccaacacc cacctctcgc tggatgctcc cttcacccag tccgaggaca atagtttgat
     6301 cgacgttttg gaggatgccg agcagccgcc gccggacgag cagttgttga ccgacgccct
     6361 gaaaatggag atcgggaagg cgctccgctc cctgaccccg cgggaggccg aggtgatcaa
     6421 actctacttc ggcctgcacc acgagaaggc actgaccttg gaagagatcg gcgcgcggtt
     6481 cagtttgaca cgcgagcgtg tccggcagat taaagagaag gcgatccgcc gattgcggca
     6541 tgcgtcgcgc tctaaagcgc tccgggcgta cttgaattag agtgttacat tgtttttctc
     6601 cttaagcccc gcgcatagcg gggcttttgc tttgcagtta agttccgggt cggtatgact
     6661 cgttccgaga atggggcggg agagaatatt ttttcacaaa ccatctattt gaaggacaat
     6721 aacttgcatt gagagatact ttccttcttt tgggagtttg ttgattgtaa tccattgctg
     6781 gttcgaaaga tagcggagtt ccgggaaggg ggcggaggtt caatacggtg attggtgatg
     6841 ggtgatagga gataggtgat aggtgatggt tcggccaagc tcaccacaag taggtggtgg
     6901 gtggtagggg tatgaaaaac ggatctggga tacgggaatt gggatttggg tcatacaaaa
     6961 gtcgatccca ccacagagac acagagagca cagagtcaaa acgagtagcc aggtgccagt
     7021 ggccagccga agcggagcga aggccccgcc aaaggcgggg tagccagaag gccaaacgtt
     7081 accatacatc ctcctacccc gcatttggcc ccgcatttgt gcgggatctg cgacgggatg
     7141 ttctactatt tcgcactatg tccgttttac ccccatggcg accccaaatt agcgaggctg
     7201 gaagcctcgc ctatggggga aaaggagccg gtttaccccc atggcaaccc caaattagcg
     7261 ggggaagggg cacctatgtg cccccggaac gaccccaatt ttcaggagtt ctaaaagttt
     7321 cgcctgcgcg ggtaagatga atggtcatgc ttatgtaaga cactcccata caatggattg
     7381 gaaaagaggt tgaaacaagt ccccaatctc cgggtgatcc gaagggtttc agttaggcca
     7441 agctgcacat agtgttgact gctggttgac tctttaggga tggggctaaa gagttaaaga
     7501 gcaaggcaat gaaaattgct tattatcagg tgaaagaatc cttgacaaga tacacccggg
     7561 cactctataa tagatacctt tcaaaggtgg cgttctttcc gtgcgggtac gcgcaaaaac
     7621 ccgtgggaaa cgtttgaaag ttcgacaccg aaagacgggg tgcaattcta acttttttga
     7681 gggaggtttt atgtcggggc gtgattggtt tactatccga ggacagcgaa gtgtctgtat
     7741 tcttctcatc ggcggtgtgc tttgctgtac tggaactaca gatgacgtgt atgcagggcc
     7801 attcgtagac tcggtcattg tgtcctccaa gattttcgct tccggaagcc aagacgttat
     7861 cgaggtaaag attcacaatg attctacgct aaatggttgg acaattccct tagttatcag
     7921 gtcgcaaacg ggcggagcat tcgggagagt cgattcagtg atatcggtcg ctccattcgg
     7981 gggcgcccgc gtgcttaaag tcaacggtgt cactccagat tctttggtca tcactggagt
     8041 gtcccttggg gttgcccccg gcccacggcg gacagcgttc agactcttcg tgactaataa
     8101 tagccagttc ggaacctatt cgctggactc caaccggctt tcggctagtc aggcacttaa
     8161 gctcacccac ccgccagcgc agccaatcgt tccagttttc gtccgcgggt tagagtcaat
     8221 cgcgaccgga aatttcccag atctacatgc tactgtcgga ggaagccgtg cgcgacccgg
     8281 tttttcgaaa ctctacggga taagttacgg gaatattggt tctttaacca ccatcggtac
     8341 ggtgacttta gcgctccctc gacaagtaat ccaccagtca agcagcgacg gaggcgtgta
     8401 taaccctggt acacatagcg tgacgtggaa tgtcgggcca ttagcccccg gatttattga
     8461 ctggcttgtg acagtggttg gaattcccaa ttcagtacca ttgggaacaa ccctaatatc
     8521 aagtgtttac attgaggcat taggcggcga tcctaatccc ggaaacaata cggactggga
     8581 gagccaattc gtgaggggtg ctttcgatcc aaatgaaaag ttagtgtcac ctcaaggcga
     8641 gggcccgttt ggcctaattt ccagaactga tacgctcaga tatcaagtta acttccaaaa
     8701 cgtgggcact gacacggcgt tcgacattgt agtgcgtgat acgttggaca acaatctgga
     8761 tgaagcaacc gtcgaaagtg gagcaagtag ccatccctat gtgttcagta ttgctggcag
     8821 ggagttgagc tggacattta cgaatattaa ccttccagac agcattgtag acgaactcgg
     8881 cagccacggt tttgtaacct ataaagttcg acccaaggca aatgcaacca gtggcacgga
     8941 catcaaaaac cgcgcagcgg tctacttcga ctttaatcca ccagtcatta ccaatacggt
     9001 atctaaccgt atttgcgacc ggcgtggtga tgttgctggt gatcccaata ctaactgtcc
     9061 ggataggacg attgacatcg tggacattgt agcggtgatc aacaacgtcg tcttcaacac
     9121 acccttctgc gggccggctg atgtggcgga tgtcgcggga gatccaaatg gctgcccgga
     9181 cacgaacgtt gacatcgtag acattgtctc catcatcaac aacgtggtgt tcgggacgcc
     9241 gttctgcctg gagtgcctat aaaagacagg gattagggat cggggattgg ggatcagaaa
     9301 gaagcacaaa aagccctgcc caaaggtggg gcttttttat ttacctgccc tcaggggctc
     9361 aaaaattttt gcttaatttc tgcggattgg gaagccgata aatcatttaa ggcagcgtaa
     9421 gcaagcctca gcctgagcca aaatagtaat cccggttttc ttcctgaaaa gagaaaagac
     9481 ctgaccgtag ctagtcactc tctgtaaaag ctaatcttcc gggtcgctgc ctgaagctgc
     9541 ctggggttgc ccttcgcccc ggttcgtctc taatgagttc ttcgtcgcaa tcctcctggc
     9601 tgggtaatga aaccgaaagg agcgatgatg atgacaagat gcgtacggtt cgcagtgttg
     9661 ggaatggtcg gcaccggctt cgcgtgggcc agtgctgtct ttgcggtacc ccctccgaac
     9721 gataattgct ccaatgccat cagcatccga gaggtggcga acatcccgtt cagcaccgag
     9781 ggcgcgacct tcgacggccc cggcgggtgc atgacgtcgc ccaacatctg gtaccgctac
     9841 accgcctcct gcaccggccc ggttaccgtt agtctctgcg gttcgaacta cgacacgaaa
     9901 ttagcagtgt atagcgggag ttgtggttcg ttcggcgctc cgcttttttg caacgacaac
     9961 ttctgcggat tgcagtcgcg cgggacgttt acggcaattg cggggaatac ttatctggtc
    10021 gaagtgggcg gagccggcag cgccaccggg ctgggggtgt tgagcgtctg ctgtggtccc
    10081 ccgccgtcca atgataactg cgccaacgcc acgcccatcg gcgaggtcac cgacctgccg
    10141 tactcgactt tgtgcgcctc gttcgacggc ccgggcggct gtatcacctc gcccaacgtc
    10201 tggttccgct ataccgcctc ctgcgacaaa gcggtgagcg tcagtctgtg cggctccgac
    10261 ttcgacacct tccttggcgt tttcgacggc ggcggctgca atccggtcgg tcccccggta
    10321 ctctgccaag acgactactg cggcctgtca tcgcacggca cgttccgggc acaaggggga
    10381 cacacctatt tgatcgaaat cggcggctac gtcggcacca ccggcacggg gactttgacc
    10441 atctcctgct gcgttccgcg cggggatgtg gccggcatgg ccgtcaccaa ctgcccagat
    10501 ggtgtggtcg gcatcgtgga tgtaattgcg gtgattaaca aagtggtgtg gggtattcca
    10561 tattgtggac cagtcatcgc cggcgacgta ggcggggcgc ccgacaccaa ctgccccgat
    10621 ggagtcgtga acattgcgga tattctcacc ctgataaagt actcagtgtt cgcgataccg
    10681 ctttgcgagg aatgtctatg aaaggcggag atcagggatt ggggattagg gatcagtaac
    10741 aaaaggccaa gtactccgct ctcaagttag gttttttcca tcttgacgcc ccttggcgcg
    10801 tcgaggatat tagttgccag ccccaatagc tcaactggac agagcatctg gcttcggacc
    10861 agagggttgg gggttcgagt cctccttggg gcatcgaaga ccccgaacgt agttgagggg
    10921 aacggtatgg gaaaccgtct gctcgcaaat ctcggtacgc tcggccacgc gatactccaa
    10981 ttcatttacc cgctggagtg ctccctctgc gaacaaccgg tcgaagagca caacgcgccg
    11041 ctctgcgaaa agtgcttcgc ctctctcgcc acggttgacc agcctttctg ccttttctgc
    11101 cacaagtttt tggggccgac ggttgcctgc gttagttgcg attccggatt gccccaggta
    11161 ccgaccctct ccctcggcgc ctttgacgac tcctaccaga aattgatcca ccagttgaag
    11221 taccgcaatg cccgtccttt gggaaaactc ttcggtcagc gcatggccga gtgtcttggg
    11281 caattggaac atttcggccc gattgatgtc gtcatcccag tcccgctcca ccgccgacgg
    11341 aaatggaagc gcggattcaa tcagagcgat ctcgtggcgg aaggaatcgc gaaagcgctg
    11401 ggcaaaccac tggacaaacg cactctgcgc cgactccggc atacgcaaga ccacacccgc
    11461 ctctcccgtg tcgaaaggca gcgcaacgtg gccggcgcct ttggcgtggt aaatcctgat
    11521 agaattcaag gcaaaaccgt actcttggtg gatgacgtca ccaccaccgg cgcgacgttg
    11581 ggtgagtgcg cgcgaacatt agtgaacgcc ggtgccaaac gcgtttgcgc ggcggcgatt
    11641 gccgtagcac tttgacgttg gaaatcccta tattaccccg cgcgggcgac ccgcacgggt
    11701 cgccccacga cttgatctga aattgagact ctgaaaggag catcggtggt agacactaac
    11761 ctgttcaacc gcatcaccgc ccgcattgac gcctcaaaac cggcgatgat tgatctgcaa
    11821 aagaaactct ccgccattcc ggccatcggc ccgaagaacg atggcaaagg cgaatgggag
    11881 aaatcgcgtt tcctgaaatc gtacttggag caaaacggct tcgcgcaaat tcaggagtac
    11941 gacgcgcccg atccgtcggt gccgaacggc gtgcggccca acttcgtggt gaaagttccg
    12001 ggacggtcga gttcaaagac agtctggatt atgacccata tggacgtggt gccgcccggc
    12061 gagcgcacac tctgggaaaa tgaccctttc acggtcatcg agaaagacgg caaactgatt
    12121 ggccggggcg tggaggataa ccagcagggc ttggtcagtt ccctttttgc ggtcaaggcc
    12181 tttttggacg aaaaaatcac tccagcgttt gacaccggcc tcatcttcgt ttcggacgaa
    12241 gaaaccggga gcgaactggg catcggctac ctgctcaaaa actttcaact cttcaagccg
    12301 gaggatttgg tcatcgtgcc ggatgcgggg aacgaaaaag ggacgctctt ggaagtggcg
    12361 gaaaagtcaa tctgctggct caaattcacc accaaaggta agcagtgcca cgccagtatg
    12421 ccccacaagg gggtaaactc cctgcgcgcg gcctcgcatt tgatcatccg gctgggagaa
    12481 ctgtaccggc tctaccccat caaagacgag atgttcgacc cgcccctttc gacctttgag
    12541 ccgaccaaaa aggaagcgaa cgtcccgaac gtcaacacca ttcccggcga cgacgtcttt
    12601 tacctcgact gccgcatcct gccgcagtat aaagtcgtgg acgtggtcaa gacgatccgc
    12661 cggatctgcg acgaagtgga gcaggagttt aaggtgacga ttgcgattgc ctcacagcag
    12721 ggcgagcagg ccgccccgcc cacgccggcg aacgcgccgg tggtgcaggc gctcaagcgg
    12781 gccatcaagg acgtgtacaa agtggacgcg gttccgatgg gtattggcgg cggcacggtg
    12841 gccgcgctct tccgccgggc ggggttcccc gccgcggtct gggcgaaaat ggatgagatg
    12901 gcgcatcagc ccaatgagta ctgctggatt gacaacatag tcggcgatgc caaagttttc
    12961 gctcacgtgc tgctgcaggg atagattgta acagcaagga cgagatggcc cacttgccca
    13021 atccgcctga cgcggactcg actcttcgag aggcgtactg gattgacaat ccgccttagg
    13081 cggatgggcg acgccaaggt ctttgcgcac gtgctcttgc agtcgtagcg actcttcgag
    13141 gggtaagcac ccggactacc ttttgaaaag aggcaactgc tctcttccct tgccagggga
    13201 atcggatggg ttatattaca aatgagaaga cccggggtct tgcacggaac aagagagttc
    13261 aaagttagcc gtcgggggtt cgtcgccccg tcgaccgact ctctggccat cgtggaggat
    13321 tttatgccga tctgcataac acgccgtgat aaaagccacg cctcaaagca acccgttggc
    13381 atctcacggc ttattgacaa ttcaagactc gtttcgcggg cgtcttatac ggcacttgaa
    13441 gcggtatccg ggttgttata cggatccata caaacaaggt tcggcgctga aaagcggcgc
    13501 tcgaagggct tgacggcttt gctcgctgtt gcgcgacggt tggacaatag ccggccgcac
    13561 catttaaagg gtaattctcc atgagaaaaa cattcgtcat agccgttctg gtttttgcag
    13621 caggctctat cgccctcggg caaaaacaga gcgcgcgaag aaattcgaaa gggagcgttg
    13681 agcaggccat aagacaagtc gatcatgaac gcattcaagc gcaaatcggt gctgatgctg
    13741 tggcgcttga ccgcatctac gctgatgatt ttattggcat cgggccaagc ggcacagtga
    13801 gaaccaaagc gcaggttatt gcggatttca cgtcgggtga gttgaagttt caatctatca
    13861 ccactgacga cgttcaactg cgcgtctacg gaaacacggt cgtggagact ggccgctcaa
    13921 caatgattgg gcaagacaaa ggaaaagctg ttccccgtga caatcgcttt acgagggtct
    13981 ggataaaaca gggcaggcgt tggcggctgg ttgctaatca ctactcgtta ctgattgccc
    14041 aatgataacc agattatgca gtggtcatcg taaagcgcgc cgaacaactc gttggaccgg
    14101 agcggcggga gcgtgttttg taactaactt ggtacggcgc aggttgcgtg aaatcgcgtc
    14161 gcccggtcaa ctctgacgtt aggccattga aggccagtca caggttgccc atggtatcga
    14221 tggaggtttc ccgatgaaac agctcgcagc aatcgcactg gcgctggggt gcatggatgc
    14281 catctgctca tcaccagtgt gcgacgagcc ggcagtcgcc aagggctact gcaccggcca
    14341 ctaccagcgc tatctacgag gggcatcgct tgacgttccg atcaagaagc gtggtcc
//
LOCUS       DBNGZD010000260        14382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1569737, whole genome shotgun sequence.
ACCESSION   DBNGZD010000260 DBNGZD010000000
VERSION     DBNGZD010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 14382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14382
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1569737"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="AC16GM_06885"
     CDS             <1..972
                     /locus_tag="AC16GM_06885"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR2923435.1"
                     /translation="IIEEAANQPYVDVRLDKYETYADITPQRLPGVTAWVSIMRGCNK
                     CCTFCIVPFVRGRERCLPPEEVLKQVGQSVALGYKEIIFLGQTVNSYRFNGCDFGDLL
                     SRAAEIDGVRRIRFTSPHPIDFNDKIISVMASTEKICPQVHLPLQSASGPVLSRMKRR
                     YTIAYYDELLAKMRETIPDLAVTTDIIVGFPGETEEDFQKTYEYMAKVQYDGAFIFKY
                     SPREGTKSATWPETVSEEEKVRRLETLVKLQTEISGKLNQKWVGKRVEVLVEGQAKKS
                     KDQHYGKSPQFKTVVFDKNGENPGEFVQIFVEKATPHALVGRQAKST"
     gene            980..3955
                     /locus_tag="AC16GM_06890"
     CDS             980..3955
                     /locus_tag="AC16GM_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR2923436.1"
                     /translation="MDLILFGLVGLAALVLWVRVLALQDRYAALGLSGWGKLQIGLAL
                     FVGGALYIILQRALGLTELLTLFGLPIGLLGVASAWAVGLFLIFSSPVRWLSALRARQ
                     EQTATREDELSLLESIREAAWQPYTLVEILQYALKEMLRFSGVEAGIIFVANLTRKEL
                     ILAAASGLSREQMKRFEKIALGGDLFSRSFTNRKPVLIGNLSLHERATRGLLQKNEFR
                     SLACVPLLVKDGVVGAGLFLSPREFALDQKQVASLAQAGNLLGSAIETARLQREVARR
                     TERVGQLEEGYKSITDLISFVWQAAGWEQAFDRLFVALLPRLGGEVACVVQKESGDAY
                     RVIASSDPTCFGKELGSPFTPHFAEAVRTGRPLVVPGLLDTPLGDRIASAVIAPLFFW
                     GESQGFLVIGSARPDPDYSQAELAQMDFAARLAAVAATQITAQRAVVTTEDKIASLSD
                     SAAALLDSPGEKFWPEVVRQCERLIPKAGAVLVFQEARPGQLALRFANVAGTLPEVEL
                     APREGSWSKALAEHRPYLATAPGEVARLWSDLSPETRTRLAGIIGARTVTAEITLPLA
                     AGRRLVADSTLRDSPSGTAGAVLSLLTLSETTRTGEPTPPFSAVDFHLAKAFACIVSA
                     LWVRFSAPAAAGAPITGDLAAALNDFNNLLTSVLGNAQMLHAQVDHVPGESRQIFSER
                     LEHIAQAAGAAGDLLRSLPSAGSAAGLPSEAAVDLTALVRETLTKERTESLESGSIFA
                     PGRAAIHPELSSVPPVTLDPGKFKAFLGEIFKRIASRPGESVTVKTDADQKYVYLRLW
                     QKGEPPAAFGGVPSQSMEAGTARDVPVDLDLVRSFRGEASYIEDETSGRALVLRFPIA
                     RAAEKSEAEHPARTLSILAIDDQPLIRDLLVAMGANLGHTVRVAESGPAGVALFRQQR
                     FDLVITDLSMPGASGWEVSRTLKKIAPDVPIVLVTGWGHSVEGLDLKALGIDYVLPKP
                     FRLEQFAELVAQVAAQTAS"
     gene            4314..4811
                     /locus_tag="AC16GM_06895"
     CDS             4314..4811
                     /locus_tag="AC16GM_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR2923437.1"
                     /translation="MSGKQICQKLSRLQKEGAGFQELLTGAVELLHHSNPRFHWTGIY
                     ELFPDNVLRLGPFIGAPTDHVFIAVGQGVCGTAVAEKRNMNVPDVSKAANYLACSTAT
                     MSELVVLIRNGETIFAQIDIDSHEYDAFDPDTVKTVEQVADWLAGAYRQRLPVSGPKG
                     SLTKA"
     gene            4831..5829
                     /locus_tag="AC16GM_06900"
     CDS             4831..5829
                     /locus_tag="AC16GM_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR2923438.1"
                     /translation="MGTLLKTDSDQVKVIEALRHKVEEYERWFRSLDNQVRVLERERQ
                     KFSAVVNQTDVGFFVVDAALRVVWTNDVFTRRFGAATHLGTLLGTTCSQILGCRKDEC
                     ALCPAAHAFKTGTVAHHEIRLHLDGQPHQLYATAMPIKSPEGEIDEIIIMLQDITDLE
                     VLRRSQEALKQTLSLLTATLDSTADGILVVDGDGKIVSFNQKFVRMWCLPESVIAARD
                     DNQALAFVLNQLKNPEEFLRKVRELYAQPEGESYDLLEFKDGRIFERYSQPHRIGGKS
                     VGRVWSFRDITELKRQQEELSRTERLRTVGELAASIIHDLKNPMNVILGSAELLNM"
     gene            5833..6510
                     /locus_tag="AC16GM_06905"
     CDS             5833..6510
                     /locus_tag="AC16GM_06905"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2923439.1"
                     /translation="MPSERRGKYCRSIAEQIQRMLILTQDILDFSRGEMRITPAPVNL
                     LELCEEIADANQAVLARLGIRLTYAGRRESGLSPAFSLDREKFWRVMMNLISNARDAL
                     SNGGEIRLRCLIAPAEARLEVEDTGQGVPEEIRDRVFEPFVTYGKTRGTGLGLSFAKK
                     IVEAHGGTIAFTTLPGKGSTFSVTLPRQTIPETESPGSGAAPGEESTQKVIQKRVQAI
                     LTSPEKR"
     gene            6515..7585
                     /locus_tag="AC16GM_06910"
     CDS             6515..7585
                     /locus_tag="AC16GM_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR2923440.1"
                     /translation="MEAPESRTKEYYQTSLRPQAEEAVFSLDRHGCVTFWSQEAQKLY
                     GLSREGVLGRSLTVLVREEKQPELRFALDRVLAGEELVRLLVPHEKCHGRVKQVEINL
                     SPVKDRQGEVLAVSCFAHDISAKREVEEHRLAETHLQAKVDSLKVVIATLSHYLNNTM
                     AVILGRTQMIDHCVGPEGITDRTGKLAPALTSIRKGTLKIAAVLEELRELSMSDHPQY
                     FKNSQAFDLEHRLDERLKKMFPEFPELTTNGKNHTSSPANTGTDAPVQSEAGQAEPKE
                     NISAKSSNAEIGLAPHPLAKPDSVESPAEPLRLSLRAVTQPATPPTLAVPAARERGAE
                     RRWQKALGRSLRKLLLRDESDF"
     gene            7693..8838
                     /locus_tag="AC16GM_06915"
     CDS             7693..8838
                     /locus_tag="AC16GM_06915"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR2923441.1"
                     /translation="MRFVVLGAGRMGQAIAFDLARTPEVGEVVLADRNLQLAKRAASL
                     LKSPKVIPRRIDASASSDLVKFLRAADCVASALPYYLNLRAAKAAVSAKVNFCDLGGS
                     NAVVKAQLALDPQAKKAGVTVIPDCGLAPGLAGLLAANGIEQLDAVESVNLRVGGLPQ
                     HPKPPLDYLLTWSVEGLINEYTEKCTVIRQGKVASVDALDELEELTFPPPFGRMEAFH
                     TSGGASTLPQTFAGRVRNLDYKTIRYPGHCAKIKTLISLGLAESKPVPVDGQKVIPRA
                     FLKTLLEKHLEREGPDVVLVRVEVAGTHKGQKRKITYQLIDYFDTQTGLSAMMRCTGF
                     SIAVVALLLARGEITQKGALPQERAVPVDQYIAELRARGIGIKEEIL"
     gene            8896..9600
                     /locus_tag="AC16GM_06920"
     CDS             8896..9600
                     /locus_tag="AC16GM_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR2923442.1"
                     /translation="MHNFKEFEALSFDCYGTLIDWETGILNALRPVLASHNQTLDGER
                     ILKLYAEIEAKLEAGRYRTYKEILKSVVKELGRQLGFFPTTDELNCLVDSLRYWQPFP
                     DTVEALQVLKEKYRLAIISNTDDDLIALTAKHLKITFDWIITAEQVKSYKPSLRNFTF
                     ALERMGIAPAKVLHVAQSIYHDIIPARSLGLATVWVDRRKGRDGFGATPKAEGKPDLE
                     VPDLKTLVTHISNGQR"
     gene            9643..10173
                     /locus_tag="AC16GM_06925"
     CDS             9643..10173
                     /locus_tag="AC16GM_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923443.1"
                     /translation="MKRTSNLKFDVTPILQRAKHLTKPRVDGISINLPFISISLTVSD
                     NEKKIAREVLIRLRDKRVLVAWECCNNCINTALASIQDIRTLLVNKQVELPDEDSALF
                     LLFDLMLAGIRQFLTFTERFDPHIHREEYFGALGVLRGHLLRCIEEIAKVGNTTPKLS
                     NRLSFNPIWEKELYLS"
     gene            10193..10900
                     /locus_tag="AC16GM_06930"
     CDS             10193..10900
                     /locus_tag="AC16GM_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923444.1"
                     /translation="MRSYKTAAESNSEISQRYSHYINKVMRDNEKFLQYYAKDTSSEI
                     IELVNDAIDNVTFWAKRGDSENDYASHSIAFFIYHILMPFSYAMYVDLIAGNIPVCFM
                     ELRLMLESLVKCYSADLRYPNQTFFQEKLELLDKKKRKISQVMKEVGEMVGFQNNFVT
                     LWRKLSESWIHTRGISDGIVGRVVDNSNVPPWALPLPMNYSKKDLGTINELRKDLSEF
                     RSLLAATMKKYQEVIIT"
     gene            10897..11823
                     /locus_tag="AC16GM_06935"
     CDS             10897..11823
                     /locus_tag="AC16GM_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2923445.1"
                     /translation="MSAIAISNLTKHFTAKMGRERVVALSDVSFEVEPGEIFGLLGPN
                     GAGKTTLVKILLSIVRATSGEVHLLGENNSDHTIRRRVGYLPENHRFPGYLTGGQALS
                     IFGRLAGLSGSHLRRRSDELLTLVQMDRWRKTQMKKYSKGMQQRLGLAVAMVNDPDLL
                     LLDEPTDGVDPVGRKEIRDLLERLKEQGKTIFLNSHLLSEVEMVCDRVAILNQGKLIK
                     VGTVGSLTEQQCEYMIEVTAWSEAVEGRLREATRAFSRQGNRLSVTLSEPKAINPVID
                     LLRINKMEILSVIPRRETLEESFLALIARPPQ"
     gene            11820..12617
                     /locus_tag="AC16GM_06940"
     CDS             11820..12617
                     /locus_tag="AC16GM_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2923446.1"
                     /translation="MTKLFAIFEDTLREAKSRKIFLGFFIFALLVLVVVALGAQFVHR
                     SAGLQVNPTGAKLTGREMSNGVASFLSDILVFLSIFATASVFPHLLEKGTAEIYLSKP
                     VSRGQWLYGKFFSVVGVIAVFVAFVLALVWLVFFLRFGSAWAGYFLFILSTALKFAIL
                     FAFVTCITVLSRSSALALILTFILWVITGFLAVREGLYRVLKTGPVQTVLDWAYYILP
                     KPGEIDKIGEALVRGNPVPSYLPVWSSVLFAVALLLLTHRIFLKKDY"
     gene            complement(12819..13319)
                     /locus_tag="AC16GM_06945"
     CDS             complement(12819..13319)
                     /locus_tag="AC16GM_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923447.1"
                     /translation="MTQRRTGPPALFGKRSYTSLCKKVVWFSLFGAAVLLAFPKNANG
                     WSYPKDGRFKKIKYDPWFYVDERWDIRAPDKFVHAMGSYASCEVLSRVFESRPRWQVA
                     AFVMLSDVYKEYGDAFREGWSPRDLMADALGIGMWIVNRKTAGSRFYVRYDTEKVQFI
                     WALPVR"
     gene            complement(13832..14221)
                     /locus_tag="AC16GM_06950"
     CDS             complement(13832..14221)
                     /locus_tag="AC16GM_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923448.1"
                     /translation="MKNAIVILFALFGAIGCAEIAHASIFGDVGNFVLTSLFKAAGGI
                     WVVLSIVLVWMTKKHLVPLLAIERNRRYAEWIARIADEVTDDLVQKYPNERWTKFLDN
                     AVDKVMEITGISKETARRAVTSAVARK"
BASE COUNT         3110 a         4080 c         4106 g         3086 t
ORIGIN      
        1 atcatcgaag aagcagccaa tcaaccctac gtcgacgtcc ggctcgacaa gtacgagacc
       61 tacgccgaca tcaccccgca aaggcttccc ggcgtcaccg cctgggtgtc cattatgcgc
      121 gggtgcaaca agtgctgcac cttttgcatc gtcccctttg tgcgggggcg ggagcgttgc
      181 ctgccgccgg aagaagtgct caagcaggtg gggcagtcgg tggcgctggg atataaggaa
      241 atcatcttct tgggccagac ggtcaattcc tacaggttta acggctgcga tttcggcgat
      301 ctgttatcga gagcagcgga aattgacggc gtccggcgca tccgcttcac ctcgccgcac
      361 ccgattgatt tcaacgataa gatcatctcc gtcatggcct cgacagaaaa aatctgcccg
      421 caggtgcacc tgcccttgca gtcggcctcc ggtccggtgc tctcccggat gaagcggcgc
      481 tacacgatcg cttactacga tgaactctta gcgaaaatgc gggagacgat tcctgattta
      541 gcggtgacga ccgacatcat cgttggtttc cccggggaga cggaggaaga tttccagaaa
      601 acctatgaat acatggcaaa agtccagtac gacggcgcct tcattttcaa atattcgcct
      661 cgcgaaggca ccaagtcggc gacttggccg gagacggttt ccgaagaaga aaaagttcgc
      721 cggttagaaa cgctggttaa actccagacc gaaatttcag gcaaacttaa ccagaaatgg
      781 gtggggaaga gggtggaagt tctggttgaa ggccaagcga agaaatcaaa agaccagcac
      841 tacggcaaat cgccgcagtt caaaaccgtc gtcttcgaca aaaacgggga aaacccggga
      901 gagttcgtgc aaatattcgt agaaaaagcc accccgcatg cactggtggg gaggcaggcg
      961 aagtcaacct gattaagtca tggatctaat tctcttcgga ctggtcggtc ttgccgcgct
     1021 ggtactctgg gtgcgggttc tggcgctcca ggaccgctac gctgctctgg ggctttccgg
     1081 ctggggcaaa cttcagatcg gcctggccct cttcgtcggc ggagcactct acatcatcct
     1141 ccagcgcgcc ctggggctca ctgaacttct gacactcttt ggattgccca ttggtttgct
     1201 gggcgtcgct tccgcctggg cggtggggtt gttcttgatt ttctcttctc ccgtgcgctg
     1261 gctttccgcc ctccgtgcac ggcaggagca aaccgccacc cgcgaagatg aattatctct
     1321 cttagaatcg atccgcgaag ctgcctggca gccctacacg ttagtcgaaa tcctccagta
     1381 cgcgctcaag gaaatgctcc gcttctccgg ggtggaagcg ggcatcatct tcgtcgccaa
     1441 tctcacccgc aaggaattga tcttagccgc ggcctccggc ctctcccgcg agcagatgaa
     1501 acggttcgag aaaatcgctt tgggcggtga tctcttctcc cgttccttta ccaaccgaaa
     1561 gccggtgttg attggaaatc tgtcgttgca cgagcgggcc acgcgcgggc tgctccagaa
     1621 aaacgaattc cgctcgctgg cctgcgtgcc gcttctggtg aaagacggcg tggtcggcgc
     1681 cggtttattc ttgagtccgc gcgaattcgc tttggatcaa aaacaggtgg ccagtttagc
     1741 ccaggccggt aatcttttgg gttccgccat cgaaacggcg cgcctgcagc gcgaagtggc
     1801 ccggcgcaca gagcgggtcg gacaactgga agaagggtac aagagcatca ccgacttaat
     1861 ttccttcgtc tggcaggccg ccggctggga gcaggcgttc gaccggctct tcgttgcgct
     1921 tttaccgcgc ctggggggcg aagtggcctg cgtggtgcaa aaagaatcgg gagatgcgta
     1981 cagagttatc gcttccagtg atccgacctg ttttgggaaa gagctcggtt ctcccttcac
     2041 cccgcacttc gcggaggcgg tacgaacggg ccgtccgctg gtcgttcccg gcctgctcga
     2101 tacaccgctg ggtgaccgca ttgcgtcagc cgtgattgcg ccgcttttct tctggggtga
     2161 aagccagggg tttttggtca tcggttccgc ccggcccgat ccagactatt cgcaggcgga
     2221 actggcccag atggatttcg ccgcgcggct ggccgccgtg gcggccacgc agatcaccgc
     2281 ccagcgggcc gtcgttacca ccgaagacaa gattgcctcc ttgagcgaca gcgcggcagc
     2341 cttgctcgat agccccggag aaaagttctg gcccgaagta gtccggcagt gcgaacgcct
     2401 cattccaaaa gccggggctg ttttggtctt tcaagaagcc cgtccgggtc agctggcgtt
     2461 gcgttttgcc aatgtcgcgg gaacattacc ggaggtggag ctggcgcccc gggaagggtc
     2521 ctggagcaaa gcgctggccg agcaccggcc gtatctcgcc accgccccgg gggaagtggc
     2581 ccgcctctgg agcgatctct ccccggaaac gcggacgcgc ctcgccggaa taatcggagc
     2641 gaggacggtg acagccgaaa tcaccctgcc tttggccgcc ggtcgaagac tcgtcgcaga
     2701 ctcgactctt cgagactctc cgagcggaac agcgggcgcc gtcctgtcgc ttttaacgct
     2761 gtcggaaact acccgtaccg gggaaccaac tccgcccttc agtgcggtgg atttccatct
     2821 ggccaaggcg ttcgcctgca tcgtgtctgc gctctgggtg cgattttcgg cgccggccgc
     2881 cgccggggcg ccgatcaccg gtgacctggc cgccgcgctg aacgacttca acaacctcct
     2941 caccagcgtt ctggggaatg cgcaaatgct ccacgcgcag gttgaccacg tgccggggga
     3001 gagccggcag attttttccg agcgcttgga gcatatcgcg caggcggccg gcgccgcggg
     3061 agatctactg cggagcctgc cctcggccgg cagtgccgcc gggttgccgt ccgaagccgc
     3121 cgtcgattta accgctctgg tgcgggaaac cttgaccaaa gagcggacgg agtctctcga
     3181 gagcggttcg atatttgcgc ccggccgggc agccattcat ccggagttgt ccagcgtgcc
     3241 gccggtgacg ctcgatccgg ggaagttcaa agccttcctg ggtgagattt tcaaaagaat
     3301 cgcttcccgg cccggcgaat cggtcacggt gaaaaccgat gcggatcaaa agtacgtcta
     3361 cctgcggctc tggcaaaagg gggagccgcc ggcagccttc ggcggcgttc ccagccaatc
     3421 gatggaagcg ggcaccgcgc gcgacgtccc cgtcgatctc gatctggtgc gttcatttcg
     3481 cggtgaagcg agttacatcg aagacgaaac ctccggccgg gcactggtgc tgcggttccc
     3541 cattgcccgg gcggccgaaa agtcagaggc cgaacaccct gcccgcaccc tttccattct
     3601 cgccatcgac gaccagccgc tcatccgcga ccttttagtc gccatgggcg ccaatttggg
     3661 ccacacggtc agggtggccg aaagcggccc ggcgggcgtc gcccttttcc ggcagcagcg
     3721 ctttgatctg gtcattaccg acctctccat gcccggcgcc tccggctggg aagtctcccg
     3781 caccttgaaa aaaatcgcgc ccgacgtgcc gattgttttg gtcaccggct gggggcactc
     3841 ggtggagggt ttggatctga aagcgctggg gatcgattac gtcctcccca aaccgttccg
     3901 cttggaacag ttcgccgagc ttgtggcgca ggtagcggcc caaaccgcgt cgtagccgca
     3961 ttggcaggcc acaggggcct gttcaaaaat ctttcaaata acatttcgat aaggcgtttc
     4021 tcgcattgaa tttttgcagg ctgaagcctc ggtcagacgg ggggcctgac tcccggatat
     4081 gttcctgacg ctatctccct cgcgcacatc aagcgaaacc cgaatcagcc gaaaatatta
     4141 aaagaacccc tctggaggtt cgcaaggaga gcgtttcgtc agcgtgcctc cgttctccgc
     4201 aaaccacgca ccgagggagt ggaagtatgt tgcaggatta gggagcaccg gggaccggga
     4261 ggcactgtgg ctgaggcaga gttttttccc agggaatcga cgggagcgcg actgtgtccg
     4321 gcaagcagat ctgccagaaa cttagccgat tgcaaaaaga gggtgccggt ttccaggagc
     4381 tcttaaccgg cgctgtggaa ttgctgcacc actccaaccc gcgcttccac tggacgggca
     4441 tttacgaact tttcccggac aacgtcctgc ggctgggccc cttcatcggc gcgcccaccg
     4501 accacgtctt catcgcggtg gggcaggggg tctgcgggac ggcggtggcg gagaaacgca
     4561 atatgaacgt gccggacgtc tccaaggccg ctaattacct ggcctgttcg acggcaacga
     4621 tgtcggagct ggtggtgctg atccgcaacg gcgagaccat ttttgcgcaa atcgatatcg
     4681 acagccacga gtacgacgcc ttcgacccgg acaccgtcaa aacggtcgag caggtcgccg
     4741 actggttagc gggtgcttat aggcagcgtc tccctgtcag tgggcccaaa ggttctttaa
     4801 ccaaagcgta acgtcgcagg cggacacccc gtgggaactc tactcaagac agattccgac
     4861 caggtcaaag tcatcgaggc gctccggcac aaagtcgagg agtacgagcg ctggtttcgt
     4921 tcgctcgata atcaggtgcg ggtgctggag cgcgaacggc aaaagttttc ggcggtggtc
     4981 aaccagaccg acgtcggttt tttcgtggtc gatgccgccc tgcgcgtggt gtggaccaac
     5041 gacgttttca cccggcgctt tggtgccgcg acccacctgg gcacgctttt gggaactacc
     5101 tgcagccaga ttctcggttg ccggaaagac gagtgcgctc tgtgtccggc cgcacatgcc
     5161 ttcaaaaccg ggaccgtggc ccaccacgag atccgtctcc atctcgacgg ccagccgcac
     5221 caattgtacg ctaccgccat gccgatcaaa tcaccggagg gggagattga cgagatcatc
     5281 atcatgctcc aggacatcac cgacttagag gtgctgcgcc ggtcccagga ggcgctcaag
     5341 cagaccctgt cgttgctcac cgccactctg gattccaccg ccgacggcat cctcgtggtg
     5401 gacggtgacg gaaaaatcgt cagtttcaat cagaagttcg taagaatgtg gtgcctcccc
     5461 gaatcggtca tcgccgcgcg agacgacaac caggccctgg cgttcgtcct gaaccagctg
     5521 aaaaacccgg aagagttttt gcgcaaagtc cgtgaactgt acgcccagcc ggaaggcgag
     5581 agctacgacc tcctggaatt caaggacgga cggatcttcg aacgctactc ccagccgcac
     5641 cggatcggag gcaagagcgt gggacgcgtc tggagctttc gcgacatcac cgaactgaaa
     5701 cgccagcagg aggaactctc gcgcaccgaa cggctccgca ccgtgggcga gctggccgcg
     5761 tccatcatcc acgacctgaa gaacccgatg aacgtcatcc tgggctcggc ggagctgctc
     5821 aatatgtagg gtttgccctc cgaacggcgg gggaagtact gccgcagcat cgccgagcag
     5881 atccagcgga tgctgatact gacgcaggac atcttagatt tttcgcgggg tgaaatgcgg
     5941 attactccgg cgccggtgaa cctgttggag ctctgcgagg aaattgccga tgccaaccag
     6001 gcggtgctgg cccgtctggg gattcggctg acctacgccg gccgcaggga gagcggtttg
     6061 tcccccgcct tctctctgga ccgggagaaa ttctggcggg taatgatgaa cttaatcagc
     6121 aatgcccgag acgctctctc caatggcggg gagattcgcc tgcgctgtct gatcgccccg
     6181 gcggaggccc gcttggaggt ggaagacacc ggccaggggg ttccggaaga gatccgcgac
     6241 cgggtctttg agccctttgt gacctacggg aaaacccggg gcaccggtct ggggctctcc
     6301 tttgccaaaa agatcgttga ggcgcacggc ggcacgattg catttaccac gctgccgggg
     6361 aagggatcga ccttctcggt aacgcttccc cgtcagacca tcccggaaac cgaatcgccc
     6421 ggctccggtg cggcaccggg tgaagagtcg acgcagaagg tgattcagaa gcgtgtccag
     6481 gcgatactca cgtcgccaga aaagcggtga aacgatggaa gccccggaat cccgcacgaa
     6541 agaatactac cagaccagcc tccggcccca ggcggaagag gcggtcttca gcctcgaccg
     6601 gcacggctgc gtcaccttct ggagccagga agcccagaaa ctctacggcc tttcccgtga
     6661 gggggtgctg ggccggagcc tgaccgttct ggtccgggaa gagaagcagc cggaactgcg
     6721 ctttgcgcta gaccgggtgc tggccggtga agaactggtg cggcttttgg tgccccacga
     6781 gaagtgccac ggcagagtca aacaggtgga aatcaatctg tcacccgtca aggatcgtca
     6841 gggggaggtg ctggccgtct cctgcttcgc ccacgacatc agcgccaagc gcgaggtcga
     6901 agagcaccgg ctggccgaaa cccacctgca ggccaaagtc gattcactca aagtggtgat
     6961 tgccacgctc tcccactacc tcaataacac catggcggtc attttgggcc ggacccagat
     7021 gatcgaccac tgcgtgggtc cggaagggat caccgaccgc accggaaaac tggccccggc
     7081 gctcacgagc atccgcaagg gaacgcttaa aatcgccgcc gttttggaag aattgcggga
     7141 actctccatg tccgaccatc cgcagtactt caaaaactcc caggccttcg acctggaaca
     7201 ccggttggac gaacggctga agaaaatgtt tccggaattc ccggaattga ccaccaacgg
     7261 gaagaaccac acctcatctc cagcgaacac cggcacggat gcacctgtcc aaagcgaagc
     7321 gggccaagcg gagcccaaag aaaacatctc tgcgaaaagt tcgaacgcag agatcggatt
     7381 ggctccccat cctttggcga aaccggatag cgtggagagt ccggctgagc cgctccgtct
     7441 ttctcttcgg gccgtcaccc agccggcaac cccaccgacc ttggcggtgc ctgcggcccg
     7501 ggagcgcggc gccgagcgcc gctggcaaaa agcactgggc cgttccctgc ggaaattgct
     7561 cctgcgcgac gaatctgact tctaaacgtt tctctttcgc tcccggctcg gatgcgactt
     7621 aactttagtt cattcaagta ggggaagcgt ctatttgatc aatttgcttc ggtctgtttt
     7681 attccactgg caatgcgctt cgtagtttta ggtgccggcc ggatggggca ggccattgcc
     7741 ttcgatctgg cccgcacccc ggaagtgggc gaagtggtgc tggccgaccg caacctccag
     7801 ctggccaagc gggctgcctc gcttctcaaa agcccaaaag tcatcccccg gcgaatcgat
     7861 gcctccgcca gttctgatct ggtgaaattt ttgcgcgcgg ccgactgcgt cgccagcgcc
     7921 ctgccgtact acctaaatct tagagcggcg aaagcggccg tcagcgcgaa ggtcaacttt
     7981 tgcgacctcg gcggcagcaa cgccgtcgtc aaagcgcagt tagcgcttga tccacaggcg
     8041 aaaaaggcgg gggtgacggt catccccgac tgcggcctgg cgcccgggtt ggccggcctt
     8101 ttggcggcga acgggataga gcaattggac gcagtcgaat cggtgaacct gcgcgtgggc
     8161 ggattgcccc agcaccccaa accgccgctc gactacctcc tcacctggtc ggtggaggga
     8221 ctcatcaacg aatacacgga aaagtgtacc gtgatccggc aggggaaagt agcttccgtt
     8281 gacgctctcg acgaattgga ggagttgacc tttccaccgc ctttcgggcg catggaggcc
     8341 ttccacacct ccggcggcgc ttcgaccttg ccgcagacat ttgccggccg ggtgcgcaac
     8401 ctcgactaca agacgatccg ctaccccggg cactgcgcaa agattaaaac gctcatctcg
     8461 ctggggctgg ccgagtcaaa accagtgccc gttgacggtc agaaagtcat cccccgggcg
     8521 ttcttgaaga cgcttctgga gaaacacctg gagcgggaag gaccggacgt ggttctggtg
     8581 cgggtggagg tcgcgggaac gcataaagga caaaagcgga aaatcactta tcaactgatt
     8641 gactactttg atacccagac cggtctttcg gccatgatgc gctgtaccgg gttttccatc
     8701 gccgtggtgg cgctcctcct ggcccgggga gagattacac agaagggcgc ccttccccag
     8761 gagcgtgcgg tgcccgttga ccagtacatt gccgagctgc gcgctcgggg gattgggatc
     8821 aaagaagaaa tcctttgatt ttgttttgta ggggttcaat atattgaacc cctacgatta
     8881 ttggatcaaa tcccaatgca taatttcaaa gaatttgagg ccttgagctt cgactgctac
     8941 ggaacgctca tcgactggga aactggtatc ctcaacgcgt tgcgcccggt tctcgccagt
     9001 cacaaccaga ccctggatgg cgaacgaatt ctcaaactgt atgcggaaat cgaggcgaaa
     9061 ctggaggcag gaagataccg tacctataaa gaaattctca agtcagtagt caaagagctc
     9121 ggtcggcaac tcggcttttt tccaaccacc gatgagctca actgtctcgt tgattctcta
     9181 agatactggc aaccgtttcc cgatacagtt gaggcactgc aagttttgaa ggagaagtat
     9241 cgcctggcga ttatttccaa taccgatgac gatcttattg ccttgactgc gaaacatctc
     9301 aaaatcacct tcgactggat catcaccgca gagcaggtaa agtcgtataa accttcgtta
     9361 cgcaatttca cctttgcgct ggagcgaatg ggcattgctc cggcaaaggt tctccacgtg
     9421 gcacagagca tctaccatga tataatcccc gccaggagtc tgggattggc aaccgtctgg
     9481 gtcgaccgca gaaaagggcg ggacggattt ggagcgactc cgaaggcgga agggaagccc
     9541 gatctggagg tgccggattt gaagacgctg gttacccata ttagcaatgg tcaacgatga
     9601 aaagattata ttcagctaag taaggtcggg tggaggactt aaatgaaaag aacgagcaat
     9661 ttgaagtttg atgtaacacc cattttgcaa agggcaaagc acctaacaaa gccgagagtt
     9721 gacggcattt caattaatct tcctttcata agcataagct tgaccgtttc cgataatgag
     9781 aaaaaaattg ccagagaagt tcttattcga ctaagagaca agcgtgttct cgtagcgtgg
     9841 gaatgttgca ataactgcat aaatactgcc ctggcttcca tacaggatat tcgaactcta
     9901 cttgttaaca agcaagtaga attacctgat gaagattcag ctttatttct actttttgat
     9961 ttaatgttgg cgggaatacg ccagttcttg acttttacag aacgatttga tcctcatatt
    10021 caccgagaag agtattttgg ggccctgggt gtgcttcgcg gtcacctctt aagatgtatt
    10081 gaagagattg caaaggtcgg aaatactacg ccaaagctaa gcaatcgact tagtttcaat
    10141 ccgatatggg aaaaagaact gtacctttct taagaactct ggccggtaaa agatgaggag
    10201 ttataaaacg gcagcagaaa gtaattcaga aatttctcaa aggtactctc attacattaa
    10261 caaagtgatg agagataatg agaagttcct ccaatactat gcaaaagata cgagtagcga
    10321 aatcatagaa cttgttaatg atgcaataga caatgtaacg ttttgggcca agagaggaga
    10381 tagtgaaaat gattatgcta gccattcaat agcttttttc atttatcata ttctcatgcc
    10441 gttcagttac gcaatgtacg tggatttgat cgcaggtaac attcctgtat gttttatgga
    10501 acttaggtta atgctcgaat ctttagtgaa gtgctattct gcagatttaa gatatcctaa
    10561 tcaaactttt tttcaagaaa aactcgaact attggacaaa aagaaacgta agatatccca
    10621 agttatgaaa gaggtgggag agatggttgg atttcaaaat aacttcgtta ctttgtggcg
    10681 caaactttct gaaagttgga tccatacgcg aggaatttcg gatggaatcg taggtcgggt
    10741 tgttgacaat tcgaacgtgc caccgtgggc tttaccacta cctatgaatt attcaaaaaa
    10801 agatttgggg actattaatg aactgcgcaa agatctatct gaatttagaa gtctattagc
    10861 agccacgatg aaaaagtatc aggaagtgat tattacatga gtgccatagc aatcagtaac
    10921 ctcaccaagc acttcaccgc caaaatgggg cgcgagcggg tggtggcgct ttccgacgtt
    10981 tccttcgagg ttgagcccgg agaaattttc gggctcctgg gtccgaacgg cgcaggcaaa
    11041 accacactcg tcaaaattct tttgtccatc gtgcgggcca cctcggggga ggtgcatctt
    11101 ttgggcgaga acaattcgga tcacaccatc cgaaggcggg tcggctacct gccggagaac
    11161 caccgcttcc ccggctacct caccggcggg caggcgctct cgattttcgg gcggctggcg
    11221 ggactatccg gttcgcacct gcgcaggcgc agcgacgaac tcctgacgct ggtgcagatg
    11281 gatcgctggc gcaagacgca gatgaaaaaa tactcaaaag gaatgcagca gcggctcggt
    11341 ctggccgtgg ctatggtcaa cgatcccgac ctccttttgc tggacgagcc gaccgacggc
    11401 gtcgatccgg tggggcgcaa agaaatccgc gacctcctgg agcgactgaa agaacagggg
    11461 aagacgattt tcttgaactc gcaccttctg tccgaggtcg aaatggtttg cgaccgggtg
    11521 gcgattctca accagggaaa actgatcaaa gtcggcaccg tcggctcgct caccgaacag
    11581 cagtgcgagt atatgattga agtcaccgcc tggtccgagg cggtggaagg acgcttgcgc
    11641 gaagcgacca gagccttctc ccgccagggg aatcggctct ccgtcactct gtccgaaccg
    11701 aaagccatca atccggtcat tgaccttctg cgcatcaaca aaatggaaat tctctccgtc
    11761 attccgcgcc gggagacgct ggaggagagt ttcctcgcct taatcgcccg accgccgcaa
    11821 tgaccaaatt attcgccatt tttgaagaca ccctgcgcga ggcgaaaagc cgcaaaatct
    11881 ttctgggctt tttcatcttc gcacttctgg tactggttgt cgtggcctta ggcgcgcagt
    11941 tcgtccaccg aagcgccggc ttgcaggtca atcctaccgg ggcaaaactg acgggccggg
    12001 aaatgtccaa cggcgtggcg agtttcctct ccgacatatt agtcttcctt tccatcttcg
    12061 ccacggcgag cgtctttccc cacctgttgg agaagggaac ggcggagatt tacctctcca
    12121 agccggtctc gcgcgggcag tggctctacg gcaagttctt ctcggtggtc ggcgttatcg
    12181 ccgtgttcgt cgccttcgtt ctggctctgg tctggctggt cttctttttg cgcttcggca
    12241 gcgcctgggc ggggtatttt ctcttcattc tttcaacggc gctcaagttc gccattctct
    12301 tcgccttcgt cacctgcatc acggttcttt cgcgttcgtc ggccctggcg ttgattctga
    12361 ccttcatcct ctgggtcatc accggatttc tggccgtccg ggaagggttg tatcgagtgc
    12421 ttaaaaccgg accggtgcaa accgttctag actgggctta ctacattctc cccaaaccgg
    12481 gagagattga taaaatcggc gaagcgttgg tgcggggaaa ccccgtgccg tcctacctgc
    12541 cggtgtggag ttcggtgctt tttgcggtgg cccttctgct cctcacgcac cgcattttcc
    12601 tcaagaagga ttattagctt ccggtcaggc tggaagtccc gctacgggcg ggactggaag
    12661 cctgacctat ggaattggtc ggtgattact cgtacagaga tgttagggat tttccatagc
    12721 cggggcttct cggtacgagc cgtatcgggc cagtccccgt cggacatttc cggaaatggt
    12781 ttttcccgtg ttctctatgc cgtattccct gtcccgcttt atctcaccgg cagcgcccag
    12841 atgaattgca ctttctcagt gtcataccgg acgtagaagc gcgaaccggc ggttttgcgg
    12901 ttgacaatcc acatgccgat gcccagggca tccgccatta aatcgcgcgg ggaccagccc
    12961 tcgcggaagg catcaccgta ctctttgtaa acgtcggaca gcataacgaa ggccgccacc
    13021 tgccagcggg gccggctctc aaaaacgcgg gagagaacct cgcacgaagc ataggagccc
    13081 atggcgtgga cgaatttgtc cggcgcgcgg atgtcccagc gctcgtccac gtagaaccag
    13141 ggatcgtatt ttattttttt gaagcgtccg tccttggggt acgaccagcc gttggcgttt
    13201 ttgggaaagg cgagcagcac ggctgcgccg aaaagcgaga accagaccac tttcttacag
    13261 agggaggtgt aacttctttt gccgaacagg gcaggagggc ccgttcgtct ttgggtcatc
    13321 tgttcctttc gctttcattt cgagggcacg ctcttgacca taaaaaaagg cgggtccgtc
    13381 gctgaacccg ccgcgcttag gggagtggtg gaggggggat gcccgacact tgggtcaggt
    13441 ggctggttgg aaagccagcg agcaccactc ccgaggctcg aaagcgctaa tcttagccga
    13501 ataccctcct cattgttaac acttccctcc ggttaataat tgagcaatcc ctgtgccaac
    13561 cgatttccgc cgattgccgc tcttgaggta tctgtctctt aattaaagta gtatctgaac
    13621 tctttaacct atctctttaa ctgactgcaa agtgatttat ttcacattgg ttccttccgg
    13681 gtgcacgaaa gtccgcctcc ggctgataag ggtagctaga attgtcactt tttcaatact
    13741 tacaactccg gaggtgcaag ttctttcaca ctctctgcaa gccgaacggc gtcgaagccg
    13801 gagtccttta tttcagccac cttcgatttt tttactttct tgcaacggcg cttgtcaccg
    13861 cccggcgtgc ggtctctttg gaaatgccgg tgatctccat gaccttgtcc accgcgttgt
    13921 ccagaaattt tgtccagcgt tcattggggt atttctgcac cagatcgtcg gtgacttcat
    13981 cggcgatgcg cgcgatccac tcggcgtagc ggcggttgcg ctcaatggcc aggagcggca
    14041 cgaggtgctt cttggtcatc cagaccaaga cgatggagag caccacccag atgccgccgg
    14101 cggctttgaa gagactcgtc aggacgaagt tgccgacgtc gccgaagatg gaagcgtggg
    14161 caatttcggc gcatccaatt gcgccgaaga gagcgaacaa gattacgatt gcgtttttca
    14221 ctattactcc ttctgaggtt aaatacctca actaactttc ccgcctactc aggctgccga
    14281 gagcagcttc gggtagtccg gctcgtctcc gagccggacc ggcgggctcc gggacgagcc
    14341 cgcctaccat gatcgtacct attctaccta tcacctcctc ac
//
LOCUS       DBNGZD010000261         2444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_458210, whole genome shotgun sequence.
ACCESSION   DBNGZD010000261 DBNGZD010000000
VERSION     DBNGZD010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_458210"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2290)
                     /locus_tag="AC16GM_06955"
     CDS             complement(<1..2290)
                     /locus_tag="AC16GM_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR2923449.1"
                     /translation="MPKPLIAFFGTLLLLTGLVSLSSQAAEPQVLVTIEPTTAEQIAA
                     ARDLGVVAYQRWERLLLGTIPEKNLPLLRAAGLPVTVLEPQPWSGTYTLVTPRRESTR
                     KAADVAQYGEVLTTFGPGALVKNLGPEPEAELRENFHLVAVTPDPIPLELPTLVAPNP
                     AYFAGATLLDSLVSLVSTESLSTYDNRLVAFQTRYSFADSNYRARDWLVQKFHDFGYT
                     DVVVDTFYYQGRFLNNIICTKPGTAEPDAIVVVGGHFDSVVYGAGDPMIFAPGADDDG
                     TGTVGTLELARVLAQTPLRKTIKFITFNTEEQGLIGSNAVASRFRGQGKDVRLMVNMD
                     MIAYVTDTIPDFDIEMTNQWASFAQLLAAAARTFTPLIPYFTTAAANSDHWPFIQYGY
                     PAVFASEGDFNQPNWHQPTDLPGKLYFPYARDVVKAVLATMYAVSEFPAGVTNLRAAD
                     LGTGDALEVRWNHSRDSLNVQGYKICWGTVSGILDSSKVVSGTTTVDTIRGLTDGQTY
                     FISVTPLPFFTSLTSCVGPQVQAAPHRAPLAPVGIRAAPGWHSATVAWNANHEVDLTG
                     YRLYRFTGAGDTLVIPRGLPETTYVDNSMQPATRYFYQVAAVDQDGNESPLSGIVSAI
                     PVTLDQGVLYVDETGSISPNPTEPEQATFYQQVLSGFSSVYHSVLGNDSTLPTLDLLG
                     QYSTVIWLDDDPQSHLANLSLPALRRYLEFGGHLFWAGWRSSVALIGSATYPFTFTPG
                     SVAYDLLHLRSVNEKTGFPIPKD"
BASE COUNT          552 a          654 c          755 g          483 t
ORIGIN      
        1 aatctttggg tatggggaat cccgtttttt cattcacact gcggaggtgc agcaggtcgt
       61 aggccacgct tccgggggta aaggtaaagg gataggtagc agaaccaatc aaggccacac
      121 tggaacgcca gccggcccag aaaaggtgac cgccaaactc caaataccgg cgcagtgcgg
      181 gcagggagag gttggctaag tgcgactggg ggtcatcatc cagccagatc acggtcgaat
      241 attgacccaa aaggtccagg gttggcagcg tagaatcatt gcccaatacg gagtggtaga
      301 cgctactaaa tccactcaga acctgctgat agaaagtggc ctgctccggc tccgtgggat
      361 tggggctgat ggatcctgtt tcgtccacgt ataagacacc ctgatcaagg gtaaccggga
      421 tcgccgatac gattcccgag agggggcttt cattcccatc ttgatccact gcggcgacct
      481 ggtagaagta ccgcgtggcc ggctgcatgc tattgtccac ataggtggtc tcgggaagtc
      541 cccgggggat aaccagcgtg tcgccggcgc cggtgaagcg gtagagccgg tagccggtca
      601 ggtcgacttc gtggttggcg ttccaggcga cagttgcgga gtgccagcca ggggcggcgc
      661 gaatgccgac cggggccagc ggagcccggt gcggcgccgc ctgcacttgc ggtcccacac
      721 agctcgtgag actggtaaag aacggcagcg gagtcacgga aatgaaatag gtctgtccat
      781 cagtcaatcc ccggatggta tcgacggtag tggtgccgct gacgactttg ctgctgtcga
      841 gaatgcccga caccgtaccc cagcaaattt tatatccttg aacgttcaga gagtcacgcg
      901 aatgattcca acggacctcc aaagcatcac ccgtgccgag gtcggcggca cgcaggttcg
      961 tcactccagc cggaaattcg gacaccgcat acatcgtcgc caacaccgcc ttcaccacgt
     1021 cgcgcgcata cgggaaatac agtttacccg gtaagtcggt cggctggtgc caattcggct
     1081 ggttaaaatc gccttccgaa gcgaagacgg cgggatagcc gtactgaatg aacggccagt
     1141 gatcggaatt tgccgccgcg gtggtgaagt aagggatgag cggcgtaaaa gtccgcgcgg
     1201 ctgcggccag cagttgggca aaggaggccc actgattggt catttcgata tcgaagtccg
     1261 gaatggtgtc ggtgacgtaa gcgatcatat ccatattcac catgagccgg acgtctttgc
     1321 cctgccctcg gaagcgtgag gcaacggcgt tactgccgat caagccctgc tcttcggtat
     1381 tgaacgtaat gaatttgatc gtcttgcgca ggggagtttg ggccagcacc cgggccagtt
     1441 ccaaagttcc taccgtgccg gtaccatcat catcggctcc gggagcgaaa atcatcgggt
     1501 ctcctgcccc gtagacaact gagtcgaagt gtccgccgac gaccacgatg gcgtccggtt
     1561 cggcggtgcc cggcttggtg caaatgatgt tgttcaggaa acggccctgg tagtaaaagg
     1621 tatcaacgac cacgtcggtg tacccaaagt catggaactt ctgcactagc cagtctcgcg
     1681 cgcggtagtt tgaatcggcg aaactgtaac gggtttggaa agccaccagg cggttgtcgt
     1741 acgttgaaag cgactcagtc gaaaccaggg agaccaaaga atcgaggagg gtggcgccgg
     1801 caaagtaggc cgggttcggg gctaccagcg tcggcaattc cagaggaatg ggatcggggg
     1861 tgaccgccac caggtgaaag ttctcccgca gctcggcctc aggttccggc cccagatttt
     1921 tcaccagcgc accgggccca aaggtagtga gcacttcacc gtattgggct acgtcggctg
     1981 ctttcctggt gctttctctc cggggagtga ccagggtata agtacccgac cagggttgtg
     2041 gttcaagcac ggtaacgggc aagccagcgg cccggagcag cggcaggttc ttttccggga
     2101 tggtgccgag cagcagccgt tcccagcgtt gataggcaac gacacccaga tctcgggccg
     2161 ccgcgatctg ttccgctgtc gtgggctcga tggtgactaa cacctgaggt tccgccgcct
     2221 ggctggagag tgaaaccagg ccagtgagca agagcagggt tccgaaaaaa gcaatcagcg
     2281 gtttcggcat gagtttcctc ctgggggaaa gatagcacgg gtgggagaag cagtcacgtt
     2341 tctttcacaa tcacttttga gaaccgaaga tttcaaacga cgaaattttt cgtcgataaa
     2401 caaaatacgg agaaggaggt ctttttccta caagaataat gcca
//
LOCUS       DBNGZD010000262        11016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1639406, whole genome shotgun sequence.
ACCESSION   DBNGZD010000262 DBNGZD010000000
VERSION     DBNGZD010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1639406"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1639)
                     /locus_tag="AC16GM_06960"
     CDS             complement(188..1639)
                     /locus_tag="AC16GM_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HIR2923450.1"
                     /translation="MKRSQKFGWLAVSLATLVGWGWWGPINSAKADQNDYGSGLIRRG
                     YEPLSIEVWTDREDYYIGGKIKVFFRTNQDAYVALYDIDTDGYLHLLFPYNGNDRRFV
                     QGGVTYRIPDKYDDYSLWVEGPPGIEYVQAVASLDPFPLPSWPGYQRPQSDEEEDDYD
                     RSGTDSYGSDYRPGERVEGDPADYLDGLVRRVVPIERYPDRCAVDLVTFHVLRPYYYS
                     RPYPVYDPYAYDPFFDCGFVYIGYPVGSEVFIDGIFFGYAPIYVPRVTVGFHVLYVRH
                     SGFDGYSYRLNVRRNQVYRFKADSFKDWKPTRVMFKKSDRPLFDEAIRKKIILPPRTD
                     TKFKQAESASGGNRGRDGQRVEGKEKRDFQQSVPSREERVFRSEKRSDWPGAEQQRIE
                     VRKSERSGGEMRTQNSDSRQREGIQVKREGPQRDAQFRSSEREKREVRQNDGRQRVEK
                     QTRVKEVDRSERPVGGSRDEMKGEKEFHSKGKK"
     gene            complement(1677..1970)
                     /locus_tag="AC16GM_06965"
     CDS             complement(1677..1970)
                     /locus_tag="AC16GM_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923451.1"
                     /translation="MKKALLIIAAAASILLLGGCYTAYSVSARYSNRGYYRPYYRPYY
                     YYDPYYYYPRYYPSYRLHYYPYYAPYYRHYYSYDHFSPYRFKGGSKGSHRRSY"
     gene            complement(2116..2937)
                     /locus_tag="AC16GM_06970"
     CDS             complement(2116..2937)
                     /locus_tag="AC16GM_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR2923452.1"
                     /translation="MKKAYLTVIGTVLLGAVITGCTDKEIIYVTRPDTQPPATPRGVR
                     SITGDRQVVITWSGSDEVDLAGYRVWRSTVANTGPYVRIGQTVNTDFVDGSVINGNTY
                     WYAVSAFDQNGNESDLSPETVFDTPRPEGFNQTVWTMEAAPTSAGFDFSSGSRTAWDS
                     PAADIFLDEDASGVIFINAATLSVDLQDFGFTTSLDDVDFAPTSGWSQKGWVEAIKGH
                     SYVIWTANNHFAKLRITDMGTGWIIFDWAYQIDPGNAELVKPKHDDNYLKRAVSD"
     gene            complement(3180..4898)
                     /locus_tag="AC16GM_06975"
     CDS             complement(3180..4898)
                     /locus_tag="AC16GM_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923453.1"
                     /translation="MGTTFSDTQGNASDGSKHIVVDPTGAAHTAWHDTEAGGRPAAAR
                     GTYWTCVTAAGGVVNLGGAQIPSPQTAANYVSYRFVAVDATPDGRTLLAGHRSATAGT
                     PAPCAFGTTLNYNGWMGVDLGACLNSWVMSVQSCMDTSLNLVFADDATTADSTLHVTW
                     IDARQSSGPSPLCYSRSTDGGATWSVPSLLTGTGPSANSAGPGRDTLAPGSYFPGTRG
                     GSDRHMAFLSYRLIGSRKSRQLALVYGVAGAPEQPPGTQFSGNIWIRRSTDAGLSWEE
                     AKALTCFDSTSQFTAQNNQYSNMVSAIFDGNDSLHITAVFDEYDPVAQTAAFTGGVLA
                     HINAENPCVIHYITRGDFLNDPSLFGDTFGGSITHPSLATPGILDASKKNVLYAVWTQ
                     FSGSDVASQNDTSDFPFYNGEIYAAVSTDKGRSWSSRNLTQTKTPFCSPGTCASEDHA
                     WVADIVANDSLNIYYHLDKYGGQLSGGTTLYPPGNEVHLLRVSVADLPSGNALKGDQN
                     LDGVLDIVDIVTLVNCVVFGGGPNCLFPCLPDFNNDTLFDINDIAALINFVVFGNPPD
                     GFGGCG"
     gene            complement(5250..6131)
                     /locus_tag="AC16GM_06980"
     CDS             complement(5250..6131)
                     /locus_tag="AC16GM_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR2923454.1"
                     /translation="MTPHFSTFSIVALDPATGQLGIAVTSKFPAVGNGVPWAKAGVGA
                     VATQALANLAYGDDGLKMMAAGLSAEMTLQKLLEFDTLREERQVGLVDAKGGAATFTG
                     KNCFNWAGGKTGKYYACQGNILTGPDVVDSMSAAYERTAGRPLAERLLTALAAGQKVG
                     GDRRGRESAAILVVKEKGGYGGYGDRWLDLRVDDHPDPVKELLRLYYDVHQLYFGETD
                     TTKLHKVDKALCTEIQTVLKKLGYYKGEVNGAYDAATRRAFFDWQGWENLEMRWREDD
                     RMDDVVLKYMREHYGRK"
     gene            6377..7660
                     /locus_tag="AC16GM_06985"
     CDS             6377..7660
                     /locus_tag="AC16GM_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR2923455.1"
                     /translation="MKLSQASVAFYLTLLIGSKAYSAPPLFIKDGTLFTITKGTIQKG
                     SILIKDGKIADIGENLKPPKDAVVVDATGKYVMPGIIDVHSHMGVYAWPGVDANSDGN
                     EMTNPVTAQVRAADAVNIEDPAIWRAIAGGTTTVQILPGSGNLIGGQSVVVKLKPGRS
                     LDDMLFKGAPPGLKMAMGENPKRVYGSRNQSPMTRMGNFFVMRDAFIKAQEYQRKWDD
                     YNKKKEKDKGLPPERDLKLETLSDVMQGKYLVHVHCYRQDEMQRMIDIAHQFGFKIRT
                     FEHSLEAYKVADLLAKEGIAISTWPDWYGFKIEAWYGIPGNAAICAKHGVRVNIHTDS
                     PEKVQRLYVDAAKCVKAGMPKDEALKALTLNPAWSLGIDDRVGSIEKGKDADLAIFSG
                     DPLSIYSLVEKTLIDGEVVFDRSKMTYDGKPLSQK"
     gene            7766..8263
                     /locus_tag="AC16GM_06990"
     CDS             7766..8263
                     /locus_tag="AC16GM_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR2923456.1"
                     /translation="MRIFVITVATLLTAVATLAQQPAQQGAQITIAPAPEKTISLKPA
                     DLVWVDVPNLPQGVKRAVVHGDPAKGPFEAFVKFPDGTELVPHWHTANEFVTVISGSV
                     LFGFGEKADKTKAVEYGPGSYIYIPGKTLHWAWAKGELVFLQHYTGPADRHLVGEGSE
                     KAKEK"
     gene            8348..9664
                     /locus_tag="AC16GM_06995"
     CDS             8348..9664
                     /locus_tag="AC16GM_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR2923457.1"
                     /translation="MSKLIWKLRLLMALISSTALAAPIAITGGKVYPISGPVIEGGTV
                     VFDNGKITAVGKNVPVPPGAQVIDATGMTVIPGLIDSYTHLGAMEIDQVDVTRDIDEA
                     ADPIEPQLKIVDGLFPESEVIPVTRTNGIATVLVAPDENNVINGQSALINLDASKFVD
                     SIIVKSPVGMHVTLGEAPKARYGPKNQMPMSRMGTAALLRQTLIKAKEFLAKQEDYQK
                     KLQEYETKKNDPKAKKEDLKEPEKPGRDAVLEALVPVIKGELPVIVSAHRVDDIETAI
                     RIAEEFNLKLILNHCTNGYKIADLLAQKKIPVLVGPITTQPGSMETQGALYENAALLQ
                     KAGVKIAIQSGEVHNVRNLPYEAGIAVSYGLPWEEALKAITLTPAEILGVASRLGSLE
                     PGKDANIAIFDGDPLQPLSNCKKLFINGRDVPLTNYQTELYEKYNK"
     gene            9809..10648
                     /locus_tag="AC16GM_07000"
     CDS             9809..10648
                     /locus_tag="AC16GM_07000"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR2923458.1"
                     /translation="MPELPDLEVVREFLAENVAGMTIAEFKVLRPLVLRPLVQGDVAA
                     QVTDKKILSVERRGKLLLLFLSGDLVLVIHPMLAGRLQYCDPKDKLRQNTFATFTLSN
                     GKQLRYFDDETMGMIYVVEQDKLNSIPRLTSQGLDAFDPALTLDVFKERLRKYHGEIK
                     GILTRGELVAGIGNAYADEILWEAKIYPFRKRKTLSETDVQALYEAIGKVFREAVAIL
                     QKRVGSNIHLELRDFLKVHRRGKQLCPRCGEPISDVYAKQKVTSFCRKCQPGLLLDNL
                     RRR"
BASE COUNT         2413 a         3195 c         3090 g         2318 t
ORIGIN      
        1 cgcgtttggg ttcgttgccg gagttcgacc gtcgcacgat gcccgccgag gaaatactca
       61 cccacaggaa ggcgaacgca accgccgcca ccaggccaac cgagagtttc ttcagcacgc
      121 cgcctccctt gggtgggcac ttcttttcat ttttcttcga cataggcatt gcataacctc
      181 agtataacta ctttttgccc ttactgtgaa actctttttc acctttcatt tcgtcccggc
      241 tgccgccaac gggtcgttcc gaccggtcga cttccttgac gcgcgtctgt ttttctaccc
      301 gctggcggcc gtcattttga cgcacttccc gcttctcacg ttcggaggaa cggaattgcg
      361 catcgcgctg cggcccttcc cgcttcacct ggataccttc gcgctggcgt gagtcggagt
      421 tctgagtgcg catttccccg ccggatcgtt ccgatttgcg gacttcgatc cgctgctgct
      481 cagcgccggg ccagtccgac cgcttctcac tccgaaatac acgctcctcc cgagacggta
      541 cgctttgctg gaaatctctt ttctctttgc cttcaacccg ctggccgtcc cgccccctgt
      601 ttccgcccga ggcggattcc gcttgtttga actttgtatc cgtgcgcggc gggaggataa
      661 tcttcttccg aatggcctcg tcaaacaacg gcctgtcgga tttcttgaac atcaccctgg
      721 tcggtttcca gtctttgaaa ctgtcggcct tgaagcggta gacctgattc cgccgcacgt
      781 tgagccggta ggagtagccg tcgaagccgg agtgccgcac gtagagaacg tgaaacccca
      841 ccgtcacccg cgggacgtaa atcggcgcat agccgaagaa aatcccgtcg atgaagacct
      901 ccgaacccac cgggtaaccg atgtagacga acccgcagtc aaagaacggg tcgtacgcat
      961 acgggtcgta aaccgggtag ggccgggagt agtagtaagg ccgcaggacg tggaacgtca
     1021 ccaaatccac ggcacagcgg tcggggtagc gctcaatcgg caccacccgc cggacgagtc
     1081 cgtccaggta atcggccgga tcgccttcca cccgctcgcc ggggcggtaa tctgatccgt
     1141 atgaatcggt gccggaccgg tcgtaatcat cctcttcctc atcgctttgg ggacgctggt
     1201 acccgggcca gctgggaaga ggaaatggat ccagcgaggc cacggcctgc acgtattcga
     1261 tccccggcgg cccctccacc cagagggagt agtcgtcgta cttgtcgggg atgcggtagg
     1321 tgacgccgcc ctgcacaaaa cgccggtcgt tgccgttgta gggaaacaga aggtgcaggt
     1381 agccatcggt gtcgatgtcg tagagcgcca cataggcgtc ctggttggtg cggaagaaaa
     1441 ccttaatctt cccacctata tagtagtcct cgcggtcggt ccagacctca atggagagcg
     1501 gctcatagcc gcggcggata agccctgaac catagtcgtt ttgatcggct ttggccgaat
     1561 ttatcggacc ccaccagccc cagccgacca gcgtcgcgag actcactgcc agccatccga
     1621 acttctgtga gcgtttcatt tcggtcatct cctttctccc gcctaaggcg gacggattaa
     1681 taactgcgcc ggtgactgcc cttggagccg cctttgaacc ggtaagggct gaagtggtcg
     1741 taggagtaat agtgccggta gtagggcgca tagtaggggt agtagtgcaa ccggtaagac
     1801 gggtagtagc gggggtaata gtagtacggg tcgtagtagt agtatggccg gtagtagggc
     1861 cgatagtatc cccggttgct gtagcgcgcg ctcacgctgt aggccgtgta gcagccgccc
     1921 agcagtagta tgctcgccgc cgccgctatg attaacaatg cctttttcat ctcatacctc
     1981 ctcgtccttc ctgatttcat ctgctccatt tgcttcacct gtcgaagacc cttctcgcgt
     2041 tagtctctca tgcagggctg cgaatccttc tttccctgcg gcgcgacgag ggcgtcgcgc
     2101 ctacgcgagg gaacttcaat cactcacggc tcgtttcagg tagttatcat catgcttcgg
     2161 cttaaccagt tcagcgtttc ccggatcgat ctggtaggcc cagtcgaaga tgatccagcc
     2221 ggtgcccata tcagtgatac gcagcttggc gaagtgatta ttagccgtcc agatgacgta
     2281 ggagtggcct ttgatcgcct ccacccagcc cttctgcgac cagcccgaag tcggcgcaaa
     2341 atcgacgtcg tccagactgg tggtgaagcc gaaatcctgg agatcgaccg acagagtggc
     2401 ggcgttgatg aaaatgacgc cggaggcatc ctcgtcgagg aagatatcag ccgccgggct
     2461 gtcccaggca gtgcgcgatc cggaagagaa gtcaaatccg gcggaggtgg gcgccgcttc
     2521 catcgtccag acggtctggt tgaatccttc cggccttggc gtatcgaaga ccgtttccgg
     2581 tgagaggtcg ctctcgttgc cgttctggtc aaaagccgag acggcgtacc agtaggtgtt
     2641 gccgttaatc actgacccat cgacgaaatc ggtgttgacg gtctggccga ttctaacata
     2701 tggccccgtg ttcgcaacgg tgctccgcca aacccggtag ccggccagat cgacctcgtc
     2761 gctccccgac caggtgatga ctacctgtct atcgccggta atggatcgca ccccccgcgg
     2821 cgtggccggc ggctgggtat ccggccgggt gacgtaaatg atttctttgt ccgtgcagcc
     2881 ggtaataact gcccccagta gaactgttcc gatgactgtc aagtacgcct ttttcatatc
     2941 tcctcctttt ccgaaccaat aatatggcaa aggcaatgcc aaggattgcc tgaacggaaa
     3001 ggtcaataac ctattgaaca gaaatcggtt aggaaaagaa attttttgat ggagaaaacg
     3061 ttagtggaaa actgcacaaa aactgtgcga aactataact cggttttgtg cggagtaagg
     3121 aaagagcggt gggtagctaa acccaccgct ctttctagac cttctaacat ctcactttat
     3181 cacccgcacc ctccaaaacc gtccggagga ttcccgaaca ccacgaaatt gatcagcgca
     3241 gcaatatcat tgatatcgaa gagtgtgtcg ttgttgaagt caggtaggca ggggaataga
     3301 cagttgggtc cccccccgaa cactacacaa ttaacgagcg tcacgatatc gacgatgtcg
     3361 agcactccat caagattttg gtccccctta agggcgttcc ccgatggaag gtcggccact
     3421 gacacccgca acaggtggac ttcgtttccc ggcgggtaga gcgtggttcc gccggagagc
     3481 tgaccaccgt acttgtcgag gtggtaataa atattgagtg agtcgttggc cacgatgtcg
     3541 gccacccaag cgtggtcttc actggcacag gtacccggac tgcagaacgg agttttagtt
     3601 tgcgtaagat tgcggctact ccagctcctg cccttatcgg tggagacggc ggcgtagatt
     3661 tctccgttgt aaaaggggaa gtcgctagtg tcgttctggc tggccacgtc ggatccagag
     3721 aattgggtcc aaacggcgta gaggacattt tttttggacg catccaaaat tcccggagtg
     3781 gctaagctgg ggtgggtgat gctaccgccg aaagtgtccc caaagagact gggatcattc
     3841 agaaagtcgc ctctggtgat gtaatgaatg acgcagggat tctctgcgtt gatgtgtgcc
     3901 aacacaccac cggtgaaggc ggcggtctgc gccacggggt cgtattcatc gaagaccgcg
     3961 gtgatatgga gactgtcatt gccgtcgaaa atcgccgaaa ccatgttgga gtactggttg
     4021 ttctgggcgg tgaactggga agtcgagtca aaacaggtga gggctttggc ctcttcccag
     4081 gacaacccgg catcggtcga ccggcggatc cagatgttgc cggagaactg cgttcctggc
     4141 ggctgctccg gggcaccggc cacgccgtag accaaggcca gttgtctgga cttgcgggaa
     4201 ccgatcagcc tgtaactcaa aaatgccata tggcggtccg acccaccccg cgtacccggg
     4261 aagtacgaac cgggagcgag agtgtcccga cccgggccag ccgagttagc cgaaggaccg
     4321 gtgccggtca atagcgacgg gacgctccag gtagccccac catcggtcga acgtgaatag
     4381 cacaagggcg acggcccgga ggactgccgg gcatcgatcc acgtcacgtg gagtgtgctg
     4441 tcagcagtgg tggcgtcgtc ggcgaaaacc aggttcagac tggtgtccat gcagctctgg
     4501 acgctcatta cccaggagtt caaacaggca cccaggtcaa cccccatcca accgttgtag
     4561 tttagtgtgg tgccaaaagc acagggagct ggcgtcccgg ctgttgctga ccggtgaccg
     4621 gccaaaagcg tccggccgtc cggcgtcgcg tccaccgcca caaagcggta ggaaacgtaa
     4681 ttggcggccg tttggggtga cggaatctga gcaccgccca ggttgaccac ccctccggcg
     4741 gcggtaacgc aggtccagta ggtcccgcgg gccgctgccg gccggccgcc ggcctcggtg
     4801 tcgtgccagg cggtgtgagc cgcaccggtg gggtcgacaa caatgtgctt ggaaccgtca
     4861 ctggcgttac cttgcgtatc ggagaaggtg gtgcccaaaa aacggtcagt tcctatagca
     4921 ggggctggaa tgggtgcata ccgggaggcg ccgatatatc ggggaggccg cctctttgcg
     4981 gccgaaaagt aagtggtgtc cgactcgtcg gaacttactg tacgaagctg atgttcctgg
     5041 gcttgttttt tgggagtggg tagatcgctc ttggccttaa ctctgggcaa cgacacgccc
     5101 ccgaaaaaaa ccgcccagac cgctacaaaa aagacctttt tacccatctt tgtgccctcc
     5161 tttcccttgg gggttgaaca agggcacgca aaacggacag tttactctat taatttatca
     5221 gaatccttaa tatttgtcaa gttctttcgt cattttctcc cgtaatgctc ccgcatgtac
     5281 ttgagcacca cgtcatccat ccggtcgtct tcccgccagc gcatttcaag gttttcccag
     5341 ccctgccagt caaagaacgc ccgcctggtg gcggcatcgt aggcgccgtt cacctcccct
     5401 ttgtagtagc ccagtttttt gagcacggtc tggatttcgg tgcagagggc tttgtcgact
     5461 ttgtgcaatt tcgtcgtatc ggtttcgccg aagtacaact gatgcacgtc gtagtacagc
     5521 cgcagaagtt ctttgaccgg atcggggtgg tcatcaaccc gcaaatcgag ccagcggtcg
     5581 ccatagccgc cgtagccgcc cttttctttc acaaccaaga tcgctgccga ctctctcccc
     5641 cgccggtcgc ccccgacttt ttgaccggct gccaaagccg tcaaaagacg ctcggccaaa
     5701 ggacggccgg cagtgcgctc atacgccgcg ctcatactgt cgaccacatc gggcccggtg
     5761 aggatgttgc cctgacaggc gtagtatttg cccgtcttcc cgcccgccca gttaaagcag
     5821 tttttgccgg tgaaggtcgc cgccccgcct ttggcatcga ccaaccccac ctgcctttct
     5881 tcgcgcaggg tgtcgaactc gagtaacttc tgcagcgtca tctccgccga aagcccggcg
     5941 gccatcattt tcagcccgtc gtcaccgtag gccagattcg ccagggcttg agtagcgacg
     6001 gcgccgacac ccgctttggc ccagggcacg ccattaccca ccgcgggaaa ttttgatgtt
     6061 accgcgatgc ctaattgccc ggttgccgga tcaagggcaa ctatggaaaa agtcgaaaag
     6121 tgtggtgtca tccgtacctc atcctcaaaa gccggactta ttgcagcgct tattaccacc
     6181 gtgagtgtta gaaagaacaa aacgaatgcc tttttctccg tgttctccgt gcctccccgc
     6241 ctttggcgga atctgcgact gtggtagatt ttcatttctc ctccttttga cacccgtttt
     6301 tacccgacta tatttgcccg caatttatgc gttgctccgg ccgttgtcat tcgctttccc
     6361 cagtaaagga ggttgcgtga agctgtccca agcatccgtc gcgttttact tgactttgct
     6421 catcgggtca aaggcgtata gcgccccgcc gctcttcatc aaggacggca ccctgttcac
     6481 catcaccaaa gggacgatcc agaaagggtc gatcctgatc aaagacggaa aaatcgccga
     6541 catcggcgaa aacctcaagc cgcccaagga tgcggtggtg gtcgacgcca caggcaaata
     6601 tgtgatgccg gggattatcg acgtccactc ccacatgggc gtctacgcct ggccgggagt
     6661 cgatgccaac tccgacggca acgagatgac caatcccgtc accgcgcagg tgcgggcggc
     6721 ggatgcggtc aatatcgaag acccagccat ctggcgggct attgccggcg gaacgaccac
     6781 ggtgcaaatc ctgcccggct cgggcaacct gatcggcggt caaagcgtgg tggtcaaact
     6841 caaaccgggg cgctccttgg atgatatgct tttcaaaggc gcgccgccgg ggctcaaaat
     6901 ggccatgggg gaaaacccca aaagagtcta cggctcgcgc aaccagtcgc ccatgacccg
     6961 gatgggcaac ttcttcgtga tgcgggacgc ttttatcaaa gcgcaggaat accagcgcaa
     7021 gtgggatgat tacaataaaa agaaagagaa ggacaagggc ctgccgccgg agcgggattt
     7081 gaaactggaa acgctgtctg atgtgatgca ggggaaatac ctggtgcacg tccattgcta
     7141 ccggcaggac gaaatgcagc ggatgatcga catcgcccac cagttcggct ttaagattcg
     7201 gacctttgag cactcgctgg aggcctacaa agtggccgac ctgctggcga aagaaggcat
     7261 tgccatttcc acctggcccg actggtacgg cttcaaaatc gaggcctggt acggcattcc
     7321 gggcaacgcc gccatctgcg ccaaacacgg tgtgcgggtg aacatccaca ccgattcgcc
     7381 ggaaaaagtc cagcgcctgt acgtcgatgc cgccaagtgc gtcaaggccg ggatgccgaa
     7441 ggacgaagcg ctcaaggccc tcaccctcaa tccggcctgg agtctgggta ttgatgaccg
     7501 ggtcggctcg attgaaaaag gcaaggacgc cgacttggcg attttctccg gcgacccgct
     7561 ctcgatatat tcgctggtgg agaaaacgct gatcgacggc gaagtggttt tcgaccgctc
     7621 gaagatgacc tatgacggca agccgctctc gcaaaagtga cgagagttcc ctccccttga
     7681 ggggagggat caagggaggg gtcatgaact ttgacctcac cccgaccctc tcctacagga
     7741 gagggagaac cctgaggagg aaagcatgcg catcttcgta atcacagtag caactctatt
     7801 gacggctgtc gccaccctcg cgcagcagcc cgcccaacag ggagcacaaa tcactattgc
     7861 gccggctccg gaaaaaacca tctccctgaa acccgccgat ctggtctggg tcgatgtgcc
     7921 caacctgccg caaggcgtca agcgcgccgt cgtccacggc gacccggcca aggggccttt
     7981 cgaggccttc gtgaagtttc ccgatggcac cgaactggtg ccccactggc acacggccaa
     8041 cgaattcgtt accgtcatct ccggctcggt gctttttggt tttggagaga aggcagacaa
     8101 aaccaaagcc gttgagtacg gcccggggag ttacatctac attcccggca agaccctgca
     8161 ctgggcgtgg gccaaaggag aattggtttt cctccagcac tacaccggcc cggcggaccg
     8221 gcatttggtg ggtgaaggaa gtgaaaaagc aaaggagaaa tagcgagtta tagttgcgtc
     8281 aggagttact cctgacgctg tgtcgggagt aacttccgac gcgaacaacc ggaatgaagg
     8341 gaaaacaatg agtaaactga tctggaagct cagattactg atggcactca tctcaagcac
     8401 tgccctcgcc gcccccatcg ccatcaccgg cggcaaggtc taccccatct ccgggccggt
     8461 gattgaaggc gggacggtgg tcttcgacaa cggcaaaatc accgccgtgg gcaaaaacgt
     8521 tcccgttccc cccggcgcgc aggtgattga tgccaccgga atgaccgtaa tccccggctt
     8581 gatcgactcc tacacccacc tcggtgctat ggaaatcgac caggttgacg tcacccgcga
     8641 tattgacgaa gcggctgacc ccattgagcc gcagttgaaa atcgttgatg gcctcttccc
     8701 ggaaagcgag gtgattccgg tcacgcggac gaatggaatt gcgactgtcc tcgtggcgcc
     8761 ggatgaaaac aacgtcatca acggtcagag tgcgctcatt aaccttgacg cgagcaagtt
     8821 cgttgattcg attatcgtaa aatcgccggt ggggatgcac gtcaccttag gtgaagcgcc
     8881 caaggcgcgc tacgggccga aaaaccagat gccgatgtcg cgcatgggca ccgccgcgct
     8941 tttgaggcaa acgttgatta aggcgaaaga atttttagcc aagcaggaag attaccagaa
     9001 gaaattgcag gagtatgaga cgaagaagaa cgaccccaag gcaaaaaagg aagatttgaa
     9061 agagccggaa aaaccgggcc gcgatgcggt gcttgaagcg ctggtgccgg tcatcaaggg
     9121 cgaactgccg gtgatcgtct ccgctcaccg ggtggacgac atcgaaaccg ccatccgcat
     9181 cgccgaagaa ttcaatctaa aattgatttt gaaccactgc accaacggct acaagattgc
     9241 cgacctttta gcacagaaga aaattccggt gttagtcggc cccatcacca cccagcccgg
     9301 ctcaatggag acgcagggcg cactgtacga aaatgcggcg ctcttgcaga aagcgggcgt
     9361 caaaatcgcc atccagtcgg gcgaggtgca caacgtccgc aacctgccct acgaggcggg
     9421 cattgccgtc tcctacggtc tgccatggga ggaagcgctg aaggcgatta cgctcacccc
     9481 ggcggaaatt ctcggcgtgg ccagtcgcct cggctcactg gaaccgggca aagacgccaa
     9541 catcgcgatt ttcgacggcg atccgttaca gcccctctca aattgcaaaa agctgttcat
     9601 caacggcaga gacgttccgc tgacgaacta ccagacggaa ctgtatgaga agtataacaa
     9661 gtaaatgctg aatcctattc ctttgacctc accccggccc tctcctatag gagagggagc
     9721 ataaaccttc tcaatacaga gttgaatttc cctcccctcg gaggggaggg ttagggtggg
     9781 gtcaaaagaa aacgtctttc aaataaacat gcccgaactc cccgacttag aagtcgtccg
     9841 cgaatttttg gccgaaaatg tcgccggcat gaccatcgcc gaattcaaag tcctgcgccc
     9901 tttagtcctt cgtccactgg tgcagggaga cgttgccgcg caggtcacgg acaaaaaaat
     9961 cctctctgtg gagcggcgcg gaaaactgct tctgctcttc ctctccggtg atctcgtctt
    10021 agtcatacac ccaatgttgg cggggcggct tcagtattgc gacccgaaag acaaactaag
    10081 gcagaacacc ttcgccactt ttactctgtc aaacggcaag caactccgct actttgacga
    10141 tgagacgatg gggatgattt acgttgtgga gcaagacaag ctcaattcca ttccccgctt
    10201 gacttctcag ggattggatg ctttcgaccc ggcattgacc ttggacgttt tcaaagaacg
    10261 cctgcgcaaa tatcacggtg aaatcaaggg catcctcacc cgcggcgaac tagtggcggg
    10321 catcggcaac gcctacgccg acgaaatcct ctgggaagcg aaaatctacc ctttccgcaa
    10381 acggaagacc ctttcggaaa ccgacgtgca agcactctac gaagccatcg ggaaagtatt
    10441 tcgcgaagcc gttgccatct tgcagaagcg tgtcggcagc aacatccact tggaactgcg
    10501 cgacttcctc aaagtccacc ggcggggcaa gcaactctgt ccgcgctgcg gcgagccgat
    10561 ttcggatgtg tacgccaagc agaaagtgac ctctttctgc cggaagtgcc agccggggtt
    10621 gctgctggac aacctgcgcc ggagatagac cccccctgcc ctcccccgcc agaggcgggg
    10681 tcttcgaccc tcaagggtag ggagattcaa ctttcttttg aaaatgttta cactccctct
    10741 cctgtaggag agggtggggg tgaggtcata gtagcaagac taaccccaac ttctcctatc
    10801 ttgacactcg tccgccaagc cggttagttt cacggtgtgc ccgtaagaga atctcctttt
    10861 ctgcgccttc tggcgcgcct ccgtttgagg accggtattg cgctggcggc ggttttggcg
    10921 gtaatccttg tcgtcgtcgc ttacttttca atccagaaaa gtcaaaaaga cttgctggag
    10981 gtcctgcgcc gtcaggtcga ggacatctcc gtacaa
//
LOCUS       DBNGZD010000263        16133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1500446, whole genome shotgun sequence.
ACCESSION   DBNGZD010000263 DBNGZD010000000
VERSION     DBNGZD010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 16133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16133
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1500446"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1360
                     /locus_tag="AC16GM_07005"
     CDS             242..1360
                     /locus_tag="AC16GM_07005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2923459.1"
                     /translation="MRIEEVATQSQMRAFIKFPWRIYRGNPHWVPPLLVERKEFFNPR
                     KNPFFQHAQVKYWLAYRDREVVGRIAALVNQAHNDYHQEKCGFFGFFEAVNDPDVARA
                     LYGTAENWLKAQGMALMRGPANFSTNDEVAFLLEGFDHPPTLMMPYTPPYYNELAEGF
                     GLRKAKDLYGYLIRRENLPTERILRVAERIKKKEGIVVRPLRMKEFDREVARVKEIYN
                     AAWSKNWGFVPMTDAEFNYIARQFKQIVDPDLTLIAEVGGKPAGFCLALPDINQALIR
                     LNGRLFPWGLVKLLWLTKVKKIITGLRLVTMGVVPEFQKRGIDTIFFIETFRTGVAKG
                     YRWAELSWILEDNTLMNRAAEAFGAELYKKYRIYEKPL"
     gene            complement(1391..2320)
                     /locus_tag="AC16GM_07010"
     CDS             complement(1391..2320)
                     /locus_tag="AC16GM_07010"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HIR2923460.1"
                     /translation="MRTELPATKAPLVAVGSERRWLPPLFSALRPGQWVKNFVVLAGP
                     VFAQKILEPGALGRSLLALGIFCLVSSAAYLLNDVVDLESDRRHPVKSQRPIAAGRVR
                     SGTAVGLSLFLVAAGLGLSFWLRLRFAAVVGAFLLLNLAYTYRLKNVVILDVMAIAVG
                     FVLRAISGAVAINVAISPWLVVCAILLALFLGFSKRRHELLLLEGSAEEHRKILREYS
                     PEFLDQLISVVTASTVVAYAFYTLSPEVRTKLHTPYLPLTIPFVLYGIFRYLYLTHQK
                     IEGGDPTRTLLSDRPLVLNTFLWVAAVLVILYF"
     gene            2668..2913
                     /locus_tag="AC16GM_07015"
     CDS             2668..2913
                     /locus_tag="AC16GM_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923461.1"
                     /translation="MAMKVKFTLAMEDVSVAGQHYDALVLDWVSDVSEEEVLAMSQAW
                     ITSQHFLTERMAGLKRVGESSLTIEPVEDTGSDFTND"
     gene            2931..3227
                     /locus_tag="AC16GM_07020"
     CDS             2931..3227
                     /locus_tag="AC16GM_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923462.1"
                     /translation="MDFLTNLLAFVRQFFIPVTDRAALLFGPLGPVAFWALLGSIALA
                     LLFKLAKITFDLLRLVAFPAITVALACNYLTPWPFQQVLPVVVILFSLVFLFKS"
     gene            3817..5061
                     /locus_tag="AC16GM_07025"
     CDS             3817..5061
                     /locus_tag="AC16GM_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012144092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR2923463.1"
                     /translation="MSRAMSIFLLSLVLFSRVLAQPCVDCHKKVTPSIVSDWQLSKHS
                     QNDVDCSVCHGDKHTSAQDVARVQIPTPEVCITCHEQQVQQFKAGKHAFGWAAMKAMP
                     TAHWQPMALMEGMKGCGGCHKIGLKTEAEIKELKKDGAGFGVASCDACHTRHTFSLQE
                     ASQPQACQTCHMGFDHPQWEMYSASKHGIRYLLKQNKTLPEAVAAPTCQTCHMQGGNH
                     EVRSAWGFLAVRLPLPENKEWAADQLTILQGLGVLDPEGKPTARLEIVKGADVARLTQ
                     DGWQKERDKMVVTCNQCHSVNFAKGELAKGDQIIKAADSLLAEAIRTVAALYQDGILK
                     KPKSYAYPFPDLLTFHDAPTVIEQKLFVMHLEHRMRAFQGAFHANPDYALWYGWSEMQ
                     RDLTEIKTLAADLRWNHTKTQR"
     gene            5429..6874
                     /locus_tag="AC16GM_07030"
     CDS             5429..6874
                     /locus_tag="AC16GM_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR2923464.1"
                     /translation="MNVRLSTGREIPIEMHKVRIVQKTSLAPVSERLKAIAEGGYNTF
                     SLRTRDIFIDMLTDSGTNAMSDNQLAAMMVADDAYAGCESFYKLAAAIKDVLRFDYVL
                     PVHQGRAAEHLLAKVFIKPGDVIPMNYHFTTTKAHFELAGGTVLEIYTNEALNTQSTN
                     PFKGNLDLQKFKDTIKKYGAAKIPFIRMECTTNLLGGQPFSLENLKAVKEIAAEHKIP
                     LVVDGSLLSENAYFIKQREKGYADKSIQEIIWEIMSLADLFYLSGRKSTCVRGGFIAT
                     NNKKFYEDLKPWLPVYEGFLTYGGMSTKEIEAMAVGLREMTEINVAGSAAEFVKYFVE
                     RLVESKIPVLTPPGGLACHLDAKLFLPHLPQSQYPAGAFSAAVYLASGVRTMERGTIS
                     MDRDKEGKEVYSDLELVRLAVPRRVYTMSHIEYVVDRVNWLYKHRDLIKGLKFVEEPP
                     VLRFFFGRLQPLDNWGAKLAEVFQKEFGLYY"
     gene            6927..7550
                     /locus_tag="AC16GM_07035"
     CDS             6927..7550
                     /locus_tag="AC16GM_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HIR2923465.1"
                     /translation="MKKNLVQRLLALIIVATGVFLGTNQVLAHCDGMDGPVVKAAQKA
                     LETGNVNLVLIWVQKDDEGALKEAFQKTLTVRKLSPDARELADMYFFETLVRTHRAGE
                     GAPYTGLKPAGRDLGPAIPAADKALESGGVEPLMKLLTDTLQAGIRKNFQEALAKKNF
                     TRDDVAAGREYVRAYVEFIHYVERLYAAAHGPAHGHFPEAGMHAEEH"
     gene            7586..8035
                     /locus_tag="AC16GM_07040"
     CDS             7586..8035
                     /locus_tag="AC16GM_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR2923466.1"
                     /translation="MEFEIPLPLRSEHDELHVELARATKVKGKIGTAAQAVATILHSH
                     FVKEEKYALPPLGLLPLLAKGKVTAEMAEVLTLTDRLKAELPQMLQEHRAIVAALDNL
                     LAAAKKEKKSAYARFAKNLMLHAQTEEEVFYPAALLVGEYLKLKLNR"
     gene            8088..8855
                     /locus_tag="AC16GM_07045"
     CDS             8088..8855
                     /locus_tag="AC16GM_07045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2923467.1"
                     /translation="MGRVAGKVAIVTGGALGIGQATCLLLAQEGAKVAVTDLLDREGQ
                     TVVDEITRSAGTARFWHMDVSQEAEVRQAVAAIHKTFGKIDILVNNAGIAGVSKPTHE
                     ITEEEWDQVMAVNVKGVFFCTKHVIPYMLAAGGGSIINLSSIYGIISAPDVPPYHASK
                     SAVRSMTKTDALIYGKDKIRVNSVHPGFVWTPMVENYLKSQGDVAAGRKFLDGLHPLG
                     HVGKPDDVAYGILYLASDESKFVTGSELVIDGGYTAR"
     gene            8980..10329
                     /locus_tag="AC16GM_07050"
     CDS             8980..10329
                     /locus_tag="AC16GM_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR2923468.1"
                     /translation="MASVPFNPRIFKAYDIRGIYPAEINRESAEWIGRALVGYLRPRQ
                     VVVGRDMRLSSDELFGGLVAGLRAAGTDVVDIGLCSTDALYFAVGKYGYDAGVMITAS
                     HNPKEYNGFKICRREAVPLSAGEGLDELRQRIESGDLSPAKKSGELTQRNITPAFVDH
                     LLSFIAPQKIKPFKIAIDAGNGMAGMLVPEIFSRLSCQLIPLYFELDGSFPNHPASPI
                     EPENLHDLQRKIRETGADLGAAFDGDADRMFLVDEKAKPLGGDVVTALIAKNLLRKNP
                     GATVLYNLICSRLVPEVITRAGGRAVRTRVGHAFIKPLMKEHSAIFGGEHSGHFYFRD
                     NWYADSGMIALLVCLELLSLENRPLSALVREIDPYHRSGELNSRVADQQVVIEKVVGN
                     FSDGLIDQEDGLTLQYPDWWFNLRPSNTEPLLRLNVEANSPELLGEKTALLLSLIRK"
     gene            10425..11177
                     /locus_tag="AC16GM_07055"
     CDS             10425..11177
                     /locus_tag="AC16GM_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2923469.1"
                     /translation="MGQTKKDKARVLVVDDDARIAALLSDTLAAIGYRTVSATSGSEA
                     LGCIAQTRPDIVITDLKMPEMDGFSLLREIKDKYPDLVVVAITGEILNEVEKQKAERG
                     ADGFLTKPFRIGKIEELIENLLSGAPPKRTGPSCILIVDDDPEVRQSLAKLLVEAGYE
                     TLTAPDADQGLALLATRKINFVITDVVMPGLSGPQLVRIIKSRYPDLPVALITGKTDN
                     IIPPEKDLKAADAFLHKPIRLSDLTKIIGSNT"
     gene            complement(11207..11662)
                     /locus_tag="AC16GM_07060"
     CDS             complement(11207..11662)
                     /locus_tag="AC16GM_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014037.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR2923470.1"
                     /translation="MRFICDEMLGKLARWLRVLGYDTRYFKKIDDARLVRLALEEGRS
                     ILTRDRRLSQLHLAAGALLLKSDNPLEQLQQVISHFALAREAEARFTRCLACNTLLQE
                     INREAIQDRLWPYIYQTHKEFRFCPTCNKVFWPGTHVRRMEEELELMKK"
     gene            complement(11758..12579)
                     /locus_tag="AC16GM_07065"
     CDS             complement(11758..12579)
                     /locus_tag="AC16GM_07065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR2923471.1"
                     /translation="MTGSGDRGRSSIFTEKFLATARRAAVAAALEAGNYLVRQFGKRQ
                     AVGYKGAINLVTTADRQAERRIVRRIKKVFPDHSISAEEAEKRDTGSEFRWLIDPLDG
                     TTNYAHGLPIYCVAIGLERSGEIILGVVYNPNLAELFLAVRGRGATLNGQRLRISTTR
                     RLDRSLLATGFPYDIRTSKKNNLEYFRRFALSARAVRRCGAAALDMCYVACGRFDGFW
                     ELKLAPWDVAAASLVVAEAGGTLTDFSGGPFAITKSEVVASNGKIHRQMLAVLGR"
     gene            complement(12576..13043)
                     /locus_tag="AC16GM_07070"
     CDS             complement(12576..13043)
                     /locus_tag="AC16GM_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923472.1"
                     /translation="MTAFPRGIGFAAVVVLAFLPLPAAGKDCPMCAKPIVDSLVVEVL
                     DLSDSTVTDYRCVVCAAKAVARLPKAEIRTRSAVSGKPVTITKRKSEWQVSPKSATFL
                     LLTERDGECLEVHKAFVNEKEFKDYLAGRRELASEKPKPQRIDDLKKKVAPNK"
     gene            complement(13040..14842)
                     /locus_tag="AC16GM_07075"
     CDS             complement(13040..14842)
                     /locus_tag="AC16GM_07075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2923473.1"
                     /translation="MAEQFHEEDILGKAYDSRLMKRLLTYLKPYRFHVAIAVVLLLAV
                     SLLQLAPPYLTKVAIDDYIKKGNLHGLNGLALLFILILAVQFVVRYVQTYLMQWVGQK
                     AMFDLRMGIFSHLQKLTLSFYDRNPVGRLLTRVTSDVAALNEMFTSGVVAVFGDLFTL
                     AGIVAIMLYLNWKLALVAFVAIPLLFYATLVFRRKVRAAYREVRLKVAKIMAFLQEQI
                     SGMATVQVFNQEKKVFSQFDGVNASLRDSHLRSIFYHAVFFPAVDLIGALSIALIIWY
                     GGGQVLSGFLTLGALVAFIQYVDRFYQPIRDLAEKYNILQSAMASSERIFKLLDTQPE
                     FSAKPGAKELAPVKGAIEFKNIWFAYRGEEYVLREVSFSVKPGEKIALVGATGAGKTS
                     IASLLCRFYDYQRGSILIDGTELKTINEASLRRNIGLVLQDVFLFSGDIAGNIRLGSE
                     AMPLERVEWAAREVGADRFIRNLPRRYQEEVTERGSTLSVGQKQLLAFARALAYDPKI
                     LIMDEATSSVDTETERQIQAALERLLANRTAIIIAHRLATVQMADRIIVLHKGQVREE
                     GTHQELLARRGIYYRLYLLQYKDQTERVGERAAV"
     gene            complement(14897..15598)
                     /locus_tag="AC16GM_07080"
     CDS             complement(14897..15598)
                     /locus_tag="AC16GM_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923474.1"
                     /translation="MVSTIQEGILVTKPSWVFYEDAKRLLERSKSLCEEEKQAQRFIE
                     EAHTFARSAFLMFIFSVEALANEILNSDFRVVPDDQIPENIINKFRIDKRKGVSWCSV
                     TDKVVILPYLCCTPVDWSKKFFDRGAREFQIFDELVDIRDNFVHAKPERRKIEIELSP
                     SRVHKMIDDFPENFWPTTKITKDIDLGADHVEIAKKSVEWIRFQLDTFLEGCLSKDNW
                     WRTEKIVGLATVKRG"
     gene            complement(15631..>16133)
                     /locus_tag="AC16GM_07085"
     CDS             complement(15631..>16133)
                     /locus_tag="AC16GM_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923475.1"
                     /translation="AKPYKVAFLQRAGSKVWFRWILKKILNAVGWLLAIFFGGAGWIV
                     LIIIFGVVLVYKALRALFKVTLSPFKRKKKQTIYQPAMFSSSEGLPSDVLLKEWPERD
                     LVEEDAAEAYEEPEGAGSKQSVANERELTFYCWKCGTLLSVTSTSAGQRAKCPSCNVS
                     LVVPDQ"
BASE COUNT         3769 a         4617 c         4335 g         3412 t
ORIGIN      
        1 cccgccgtgc ccaaaggccg ggccttgatc cgtaccagtt acaccgccac ccatacggac
       61 gagcagattg acaaagtgct gggcctcctg gcggaagtgg gccagcgctt cggcatcctc
      121 aagccgcaac cggtacccgt tggagatgcg taattctcca gccgccgcga ccgccggaca
      181 actcccggcc cacttcctcc attaaaacag aactgctcta ccgagagaat cacctcccgt
      241 gatgcggatt gaagaagtcg ccacccaaag ccagatgcgg gctttcatta aattcccctg
      301 gcgcatttac cggggcaatc cgcactgggt gccgccgctt ttagtggagc ggaaggaatt
      361 ttttaacccc cggaaaaatc ctttcttcca gcacgctcaa gtcaaatact ggttggccta
      421 ccgagatcgg gaggtggtcg gacgaatcgc cgccctcgtc aaccaggcgc acaacgacta
      481 ccaccaggaa aagtgcggct tcttcggctt ctttgaagcg gtcaacgatc ccgacgttgc
      541 ccgggctctc tatggaaccg ccgaaaactg gctcaaggcg caggggatgg ctctcatgcg
      601 cggcccggcg aacttttcga ccaacgatga agtcgctttt ctcctggagg gcttcgacca
      661 cccgccgacg cttatgatgc cctacacccc gccctactac aacgaattag ccgaaggctt
      721 tggtctccgg aaagccaaag acctctacgg gtatctgatt cggcgggaga atctgcccac
      781 cgagcggata ctccgggtgg ccgagcgcat aaaaaagaaa gaaggcattg tcgttcgccc
      841 gttgcggatg aaagaatttg accgtgaagt ggcccgggtg aaggaaatct acaacgccgc
      901 ctggagcaag aactggggct ttgtgccgat gaccgacgcc gagttcaatt acatcgcccg
      961 gcagttcaag cagatcgtcg atccggattt gacgctcatt gccgaagtgg gggggaagcc
     1021 cgccggcttc tgcctggccc tgcccgacat aaaccaggcg ctgatccggt tgaacggacg
     1081 gcttttcccc tggggattgg tcaaactcct ctggctgacc aaagtcaaaa aaatcatcac
     1141 cggcctgcgc ctggtcacca tgggcgtggt accggaattt cagaaacgcg gcatagacac
     1201 aatcttcttt atcgaaacct tccgcaccgg cgtggccaag gggtaccggt gggccgaact
     1261 gagctggatt ctggaagata atacattgat gaaccgcgcg gcggaagcct tcggggctga
     1321 gttatataag aagtaccgca tttacgaaaa accgctatga gtgatttgac cgtctaagtc
     1381 cgcagttttt ttagaagtag aggatcacca gcaccgcggc cacccacaaa aacgtattga
     1441 gcaccagcgg ccggtcgctg agaagggtcc gcgtcggatc ccccccttcg attttctggt
     1501 gggtgaggta gaggtagcgg aaaatcccgt agaggacaaa ggggatggtc aggggcagat
     1561 agggggtgtg gagtttggtg cgtacttccg gcgaaagggt gtagaaagca tacgccacta
     1621 ccgttgaagc ggtcaccacg ctgatcagct ggtcgaggaa ttcgggggag tattcccgca
     1681 ggattttccg gtgctcctct gcgctccctt ccaaaagaag caattcgtgc cggcgtttgc
     1741 tgaaacccaa aaaaagtgcc aggagaatgg cgcagaccac gagccagggg gatatggcga
     1801 cattgatggc caccgccccg gaaatcgccc gcaggacgaa gcccaccgca atcgccatca
     1861 cgtccaagat gacgacgttt ttcaagcggt aggtgtaagc caggttcaag agaagaaacg
     1921 ctccgacgac cgcagcaaat ctcaacctca accaaaacga tagccccagc cccgcggcaa
     1981 ccaaaaacaa actcaggcct acggctgttc cggatctgac ccgtcccgca gcaatcggcc
     2041 gttgagattt caccgggtgc cgccgatccg actccaagtc gacgacgtca ttcagcaagt
     2101 aggcggccga cgaaaccaaa cagaagatcc ccaaagccag aagcgaccgg cctaaggccc
     2161 cgggctccag aattttctgg gcgaaaacgg ggccggccaa gacgacgaag tttttcaccc
     2221 actggccggg gcgcagcgcc gaaaaaagag gcggaagcca cctgcgttcg gatcccacag
     2281 cgaccagagg cgctttcgta gctggaagct ccgtcctcat tgccatttac cggggcatga
     2341 tcccatgatt tgagaaatca aaaattcgct aaatgccatt aaacccccgc aaggaaagtg
     2401 gctacagatg aaatgtcaaa ctctcgaact tccattccgg tctaaatgtg cgccataccc
     2461 tgttgtcttt tccccacaaa atccttcgac cgctggacgt ggtctcaggc acctgcctga
     2521 ttttgatcga tgctgacgaa tcaactcttt gaaaatcgga cggttacgcc ggaacatttc
     2581 cacatttact ctgcgcacaa tgggcataac cattgcattt gcgggtgcct gagactcggt
     2641 ggcaagattg gagatcgagc gaacgagatg gccatgaaag tgaaattcac tctggcgatg
     2701 gaagacgttt cggtggcggg tcagcactac gacgcgctgg tcctggattg ggtttccgac
     2761 gtgagcgaag aagaagtgct ggccatgtcc caggcctgga ttacttccca gcacttcctg
     2821 accgagcgga tggcgggcct caagcgggtg ggcgaatcga gcctgaccat cgagccggtg
     2881 gaagataccg gctcagattt caccaacgac taaacactcg accgaatttt atggactttc
     2941 tgaccaatct tctggcgttc gtcagacaat tcttcattcc ggtgaccgac agggcggcgc
     3001 tcctgtttgg tcccttgggt cccgtggctt tttgggcgct cctgggatcc atcgccctgg
     3061 ccctcctttt taaactcgcg aaaatcactt ttgacctgtt acgcctggtc gcctttccgg
     3121 cgattaccgt cgctctggcc tgcaactacc ttactccctg gccgttccag caagtgctgc
     3181 cggtcgtcgt cattctcttt tccctggttt ttcttttcaa atcgtgacca ccagcttttc
     3241 ctcccatact gtagggcaca aatacagtat tattgcacaa tcgatcttct gactttctca
     3301 tgtctccacc taattcaatg tctgccaacg ggtaaatatc tcccgtgaag ggcattcctt
     3361 ttgcacagcc ataatcagtc gatgaaccga cttcttttta cagctcgacc gatgacatca
     3421 acggcctcca actccctctc ttccaaaccg agacggcgga cgcggacctg cccggtctgc
     3481 ggattgtctc ttgtccgaca ccaccagagc cgcacctgcc cacgctgcca gggcgttctg
     3541 aacggcgaca cctaccgatt cccaaggtgc gaaccggatg aactcagcgg cgggtagccg
     3601 ttcgtcagcg agtctctcct gattgagcaa ataggagtgg ctactattaa cccgtttcag
     3661 ctgctctacg ataaatatac ctcgttctct tttttctatt aaccgattac tccgtctaat
     3721 gcgtacatct gagagagaag ggttgcgaga gggaaaatac tgaccggagt ggtcaagccg
     3781 acgctcgatt cacttgaaca cacggaggtt acctcaatga gccgagccat gagcattttc
     3841 ctgctaagtc ttgtactctt ttcccgggtt ctcgctcaac cgtgtgtcga ctgccacaaa
     3901 aaggttacac ccagcatagt ctccgactgg cagctgagta agcacagcca gaatgacgtc
     3961 gattgctcgg tgtgtcacgg ggataaacac acctccgccc aagatgttgc tcgggtccaa
     4021 attcccacgc cggaagtatg cataacctgc catgaacagc aggtgcagca attcaaagcg
     4081 ggcaagcacg ccttcggctg ggcggcaatg aaggcgatgc ccacggcgca ctggcaaccc
     4141 atggctttga tggaagggat gaaaggatgc ggcggctgcc acaaaatcgg cttgaagacc
     4201 gaagcggaga taaaagagtt gaaaaaggac ggcgccggat ttggcgtggc ttcctgcgat
     4261 gcctgccata cccggcatac tttctccctg caggaagcca gccagccgca ggcctgccag
     4321 acctgccaca tggggttcga ccacccccag tgggagatgt attcggcatc caagcacggc
     4381 atccgctatc tcctgaaaca gaataagacc cttcccgaag cggtggccgc gcccacctgc
     4441 cagacttgcc atatgcaggg aggcaatcac gaagttcgtt ctgcctgggg atttctcgcc
     4501 gtacggctcc ccttaccgga gaataaagag tgggccgccg accagctgac catcctgcaa
     4561 ggactcggcg tacttgatcc cgagggcaaa ccgactgccc ggctggagat cgtgaaagga
     4621 gccgacgtgg cgcggttgac ccaggacggc tggcagaaag agcgcgacaa gatggtcgtt
     4681 acctgcaacc agtgccattc ggtcaacttc gccaagggag aattggccaa aggcgatcag
     4741 attatcaagg cggcggacag tctgttggct gaggcgattc ggaccgtcgc agcactctac
     4801 caagatggga ttctgaaaaa accgaaaagc tacgcctatc cgttccccga tctcctgacc
     4861 ttccacgacg cgccaacggt gattgagcag aaactcttcg tgatgcacct ggagcaccgg
     4921 atgcgcgcct tccaaggggc ctttcacgcc aacccggact acgcgctctg gtacggctgg
     4981 agcgaaatgc agcgcgacct cactgaaatc aaaacgctgg cggctgacct caggtggaat
     5041 cacacaaaga ctcaaaggtg agaagcagtc tcactccggg agaggggctg acagtgaacc
     5101 ggccggtctg tgtgccggtg ctccgatagc cgtctgcgcg ccatagtaga cggttagggg
     5161 ggcttctttc catccatttc accgaacgga ggagtacagg aaaagccaca gcgacctaag
     5221 gggaaaagtg gctttttttg tctaaaactc ttaaattcct ccgtatggca gaggagagcc
     5281 gtcgccggcg agaggccggc agccaggagc gcggtcttga attgaaccat cggcaacgga
     5341 agaatcgcga ggcagtgacg aagaatagag cacagaaact tccgaatatt ttcgtgaaat
     5401 gagaatctaa tcagcaaagg agccccagat gaatgtccga ctttccaccg gcagagagat
     5461 tcctatcgag atgcacaagg tgcggatcgt gcagaaaacc agtctggccc cggtcagcga
     5521 aagactcaag gcgatagcag aaggcgggta caataccttc tccctccgga cgagagacat
     5581 cttcatcgat atgctcaccg atagcgggac gaatgcgatg agcgacaacc agctggccgc
     5641 catgatggtt gccgacgacg cctacgccgg ctgcgaaagt ttttacaaac tggctgcggc
     5701 gataaaagac gttttacggt tcgactacgt cctgccggtg catcaggggc gggccgccga
     5761 gcatctctta gccaaggtct tcatcaagcc gggagacgtc atcccgatga actaccactt
     5821 caccaccacc aaagctcact ttgagctggc cgggggcacg gtactggaaa tttacaccaa
     5881 tgaagccttg aacacccaga gcaccaaccc gtttaaaggc aacctggact tacagaaatt
     5941 caaggatacg attaaaaaat acggggctgc aaaaatcccc ttcatccgca tggagtgcac
     6001 caccaatctc ctgggtggac agcccttttc tctggaaaat ttgaaagcgg tgaaagagat
     6061 tgccgccgag cacaagattc ctctggtggt cgatggcagt ctgctctcgg agaatgccta
     6121 cttcattaag cagcgcgaaa aggggtatgc ggacaagtca atccaggaga tcatctggga
     6181 gatcatgagt ctggcggatc tcttctacct ctccggtcgg aaaagcacct gcgtgcgggg
     6241 cggcttcatc gccaccaaca acaagaaatt ttacgaggac ctcaagccct ggctgccggt
     6301 ctacgaaggg ttcttaacct acggcggaat gtccaccaaa gagattgagg cgatggcggt
     6361 gggactgcgg gagatgaccg agatcaacgt ggccggcagt gcggccgaat tcgtgaaata
     6421 ctttgtcgag cgcttggttg agagcaagat tccggtgctc accccgccgg gtggcctggc
     6481 ctgccacctc gatgcaaagc tctttttacc ccacctgccg caatcccagt acccggccgg
     6541 ggcgttttcg gcggcggttt accttgcctc cggggtgcgg acgatggaac ggggcaccat
     6601 ctcgatggat cgggacaaag agggtaaaga agtttattca gatttagaac ttgtccggct
     6661 ggccgtgccc cgccgggtct acaccatgtc gcatatcgaa tacgtggttg accgggtgaa
     6721 ctggctctac aagcaccgtg acctgatcaa gggattgaaa tttgtggaag agccgccggt
     6781 tctcaggttt ttcttcggcc gcttacagcc gttggacaac tggggtgcaa aattagccga
     6841 ggtttttcaa aaggaattcg gattgtacta ctaaggcagt tttgaaccaa acccgaccgt
     6901 cattgcgacg gggaaaggat aatgggatga aaaagaatct agtgcagaga ttgctggcgt
     6961 tgataatcgt cgccacaggc gtgttccttg gaacgaacca ggtcctggcc cactgtgacg
     7021 gcatggatgg tcccgtggtc aaagccgccc agaaggcgct cgaaacgggg aacgtgaacc
     7081 tcgtcctcat ctgggtgcag aaagacgacg aaggcgccct taaggaagcc ttccagaaaa
     7141 ccctgaccgt aaggaaattg agcccggatg ccagagaact ggccgacatg tacttcttcg
     7201 agaccttggt gcgcacccac cgggccggtg aaggggcgcc gtataccggc ctcaagccag
     7261 ctggccgcga cctcggcccc gccattccgg ccgccgacaa ggccctggag agtggcggtg
     7321 tggagccact tatgaaactt ctgacggata ctcttcaagc cgggatccgc aaaaatttcc
     7381 aggaggcatt ggctaagaaa aatttcaccc gggatgacgt ggcggcaggt cgggagtacg
     7441 tgcgcgcgta cgtggaattc attcattacg tcgagcggct ctacgcagcg gcccacggcc
     7501 ccgcgcatgg gcattttccg gaagccggga tgcacgcgga agagcattaa gacgacgtcg
     7561 ttgactccct aaaggaggtc acacaatgga attcgaaatt cccctcccct tgagatcgga
     7621 gcatgatgag ttgcatgtgg agttggcccg ggccaccaaa gtgaagggca aaatcggaac
     7681 cgctgcccag gccgtagcca cgatcctgca ttcccatttt gtgaaagagg aaaaatatgc
     7741 gttacccccc ctggggctct tgcctctcct ggcgaaagga aaagtcacag cggagatggc
     7801 ggaggtcctg accctgaccg accgcctgaa agcggagctg ccgcagatgc tccaggagca
     7861 tagagccatc gtcgcggcgc tggacaacct tctcgctgcc gccaaaaaag aaaagaagtc
     7921 agcctatgcc cgctttgcca aaaatctgat gctgcacgcc cagaccgagg aggaggtctt
     7981 ctacccagcg gcacttttgg tcggagagta tctgaagttg aaactcaacc ggtaggcgct
     8041 tcgggcagga gttgaaaacc tgcggcggct aacccaggga ggcgaacatg ggcagagtag
     8101 caggcaaagt agccatcgtc actggcggcg cactgggcat cgggcaggca acctgtctgc
     8161 tcttggccca ggaaggagct aaagttgcgg tgacagacct cctcgaccga gaaggacaaa
     8221 cggttgtgga tgaaatcact cgctccgccg gcacggcccg gttttggcat atggatgttt
     8281 cccaggaagc ggaagttcgg caggcagtcg ctgctattca taagactttc gggaaaatag
     8341 acatcctggt caacaacgcc gggatcgccg gggtaagcaa gccgacccac gagataaccg
     8401 aggaagaatg ggatcaggtg atggccgtca acgtgaaggg agtcttcttc tgcaccaagc
     8461 acgtgatccc ttacatgctg gcggccgggg gcgggagcat catcaacctc tcctccattt
     8521 atggcatcat cagtgcgccg gacgttccgc cgtaccacgc ctccaagagc gccgtacgat
     8581 ccatgactaa aacggatgcc ctcatttacg gcaaggacaa gatccgggtc aactccgtcc
     8641 accccggctt cgtctggacg ccgatggtgg aaaactacct gaaatctcaa ggtgacgttg
     8701 cggccgggcg gaaattcctg gatggcctgc accccctggg tcatgtgggt aagccggacg
     8761 acgtggccta cggcattctc tatctcgcct ccgacgaatc taagttcgtc acgggaagcg
     8821 aactggtaat cgacggcggc tatacggcgc gctgaattct actggatcaa cctgcttcgc
     8881 aaaatttgat tcccgtttca cctccttttc ctaaacggta ctagaagaga tatttcttga
     8941 ccacagcaga gtctttttca ttcagtatat tcagcagtca tggcgtctgt tccgttcaat
     9001 ccccgcattt tcaaagccta cgatatccgg ggcatttatc ccgcagagat aaatcgggag
     9061 tcggcagagt ggatcggtcg agcgctggtg ggctacctcc gaccgcggca ggtggtggtg
     9121 gggcgggata tgcgtttgtc gtccgacgag cttttcggcg gattggttgc gggcttgcga
     9181 gccgccggca ccgacgtggt agacatcggc ctctgctcca ccgacgccct ctacttcgca
     9241 gtgggaaaat acggttatga cgccggtgta atgattacgg cctcccacaa ccccaaggag
     9301 tacaatggct tcaaaatctg ccgccgggaa gccgtccccc tttcggcagg ggaagggtta
     9361 gacgagctgc gccagcggat tgaaagcggt gacctgtcgc cggctaaaaa atcaggcgaa
     9421 ctgacccaaa ggaacatcac tccggctttc gtcgatcatc ttttgtcatt catcgccccg
     9481 cagaaaatca aaccgttcaa aattgccatt gatgccggca acggcatggc ggggatgctc
     9541 gtaccggaaa tcttctcccg actgtcctgt cagttgatcc ccctgtactt tgaactggac
     9601 ggctcgttcc ccaatcaccc agccagcccc attgaaccgg aaaatctgca tgacctccag
     9661 agaaaaattc gagaaaccgg tgctgatctt ggggctgctt ttgacggcga tgccgaccgg
     9721 atgttcttgg tggacgaaaa ggcaaaaccc ctcggcggcg acgtggtcac cgcactcatc
     9781 gccaaaaatc tgctcaggaa aaaccccgga gccaccgtgc tctacaacct catctgctcc
     9841 cggctggtgc cggaggtaat tacccgcgca ggcggccggg ccgtccgcac ccgggtgggg
     9901 cacgccttca tcaaaccttt gatgaaagaa cacagcgcca tcttcggggg ggaacactcc
     9961 ggccattttt acttccggga taactggtac gccgacagcg ggatgattgc ccttttggtc
    10021 tgcctggaac tcctgtccct tgagaaccgt cccctttcgg ctttagtccg ggaaatcgat
    10081 ccctaccacc gctccggaga gctcaattcg cgggtggctg accagcaagt cgttatcgaa
    10141 aaggtggtcg ggaatttttc cgatggcctc atcgatcaag aagacggctt aaccctgcaa
    10201 taccccgact ggtggttcaa cctgcgccct tccaacaccg aaccgctctt gcggttgaat
    10261 gtcgaggcca attcacccga attgttaggg gaaaaaaccg ccctcctctt atcacttatc
    10321 agaaaataat ctccggtcgc gcaagcagtt acccatgacc gaggggctgg tgggagtgaa
    10381 cgattttgca cttctgccga taatgcagac agaaggttct caagatgggg cagaccaaaa
    10441 aagacaaggc cagggttttg gtcgttgacg atgatgcccg catagccgcg ctcctctccg
    10501 acacgctggc ggcgattggc tatcggactg tgagcgccac cagtggttcg gaggcgttag
    10561 gctgcatcgc gcaaacccgt cccgacatcg tcatcaccga cctgaagatg ccggagatgg
    10621 acggtttctc ccttttgcgc gaaattaaag ataaataccc ggacctcgtg gtggtcgcca
    10681 taaccggcga aatcttaaac gaagtcgaaa aacaaaaagc cgagcggggg gccgacggat
    10741 ttttgaccaa gccgttccgt atcggcaaaa tcgaggaact gattgaaaat cttctctcgg
    10801 gtgccccgcc caaaaggacc gggccttcct gcatcctgat cgtggacgac gaccccgaag
    10861 tgcgccagag tttggccaaa ctcttggtcg aggccggcta cgaaaccctc accgcccccg
    10921 acgccgacca ggggctggcg cttttagcca cccggaaaat taacttcgtc atcaccgacg
    10981 tggtgatgcc cgggctctcc ggcccgcaac tggtgcggat cattaagagc cgctaccctg
    11041 acctgccggt ggcgctcata acgggcaaga ccgataacat catccccccc gagaaggatt
    11101 tgaaagcagc cgatgctttc ctgcacaagc cgatacgctt gagcgacctg accaaaatta
    11161 tcggctccaa cacgtagcgc tgagctagac ccacttcgct gtccgtttat ttcttcatta
    11221 gttctaattc ttcctccatc ctccggacgt gtgtccccgg ccagaaaacc ttgttgcagg
    11281 tcggacaaaa gcgaaattct ttgtgggtct ggtagatgta cggccagagg cggtcctgga
    11341 tggcttcccg gtttatttcc tggagcaggg tattgcaggc cagacagcgg gtgaatcggg
    11401 cttcggcctc ccgcgcgaga gcaaaatgag aaatcacctg ctgcagctgt tccaggggat
    11461 tgtcactttt aagcagcaac gctccggcgg ccaggtgcag ttgactcaag cgccggtcgc
    11521 gggtcaaaat cgaacgcccc tcttccaggg cgagtcgcac cagccgcgcg tcgtctattt
    11581 ttttgaaata gcgggtgtcg taccccaaaa cccgtagcca gcgggctagc ttccccagca
    11641 tttcgtcgca gatgaagcgc atggcacacc gggaagaaga tagaaagacg caagtcttac
    11701 cgcacttctc cggcttttct atctgctaaa tctgcttcat ctgctgtgag tggttcttta
    11761 acggcccaaa accgccagca tctgtcgatg aattttgccg ttggaggcca ccacttcgga
    11821 tttggtgatg gcgaacgggc cgccggaaaa atcagtgagg gttccgcccg cttcggcgac
    11881 cacgagactc gccgccgcta cgtcccaggg cgccagtttc aattcccaaa agccgtcgaa
    11941 ccggccgcag gcaacgtagc acatatctaa agccgccgca ccgcagcgcc ggactgcccg
    12001 cgctgagagc gcaaagcggc ggaagtattc cagattgttt ttcttcgagg tgcgaatatc
    12061 ataggggaag ccggtggcta aaaggctccg atctaaacgc cgggtggtgg agatccgcag
    12121 gcgttggcca ttcaacgtgg ccccccgacc ccggactgcc aggaagagct ccgccagatt
    12181 cggattgtag actaccccta agataatttc accggagcgc tccagcccga tcgccacgca
    12241 gtaaatcggc aggccatggg cgtagttggt ggtgccgtcc agaggatcga tgagccagcg
    12301 gaattcggag ccggtatccc gcttttcggc ctcttccgcg gaaatcgagt ggtcggggaa
    12361 gaccttttta atccggcgga cgattctccg ttccgcctgc ctgtcggcgg tcgtaaccag
    12421 attgatcgcc cctttatacc cgaccgcctg gcgctttccg aactgccgga ctaaataatt
    12481 tcccgcttcc agcgccgccg ccaccgccgc acgccgggct gtggcaagga atttctccgt
    12541 aaaaatggaa gaacgacccc gatcgccgga cccagtcatt tattcggagc aacctttttc
    12601 ttcaggtcgt cgatgcgttg gggtttcggt ttttctgagg ccaattcccg ccgtcccgcc
    12661 agataatctt tgaattcttt ttcgttgaca aacgccttat gcacctccag gcattcgcca
    12721 tcccgctctg tgagcaaaag gaaggtggca gatttcggcg acacctgcca ctcgcttttt
    12781 cttttagtaa tcgtcaccgg tttgccagag accgccgaac gcgtcctgat ctccgctttt
    12841 ggcagcctgg ccactgcctt ggcggcgcag acgacacagc ggtagtcggt caccgtggaa
    12901 tcggaaaggt cgagcacctc caccaccaac gaatcgacta ttggcttggc acacatcggg
    12961 caatctttgc ccgcggcggg cagcggaagg aaagcgagaa ccaccaccgc cgcgaatcca
    13021 attcccctcg gaaaagcggt cacaccgccg cccgttctcc cacgcgctct gtctggtctt
    13081 tgtactgcaa gaggtagagc cggtagtata tgccccgccg ggccaagagt tcctggtggg
    13141 tgccttcctc ccgcacctgt cctttgtgca atacgatgat ccggtcggcc atctgcacgg
    13201 tggccagccg gtgggcgatg atgatggcgg ttcgattcgc caacagacgc tccagcgccg
    13261 cctgaatttg tctttcggtt tcggtatcga ccgacgatgt cgcctcatcc atgatgagga
    13321 ttttcggatc gtaggccagg gcccgcgcaa aagctaaaag ttgtttctgc cccaccgaaa
    13381 gggtactccc ccgctccgtt acctcctcct ggtatctccg gggaaggttg cggatgaacc
    13441 ggtccgcccc gacttcccgg gccgcccatt ccacccgttc caaaggcatc gcctcgctgc
    13501 ccaggcggat gttcccggca atgtcgcccg aaaagaggaa cacatcctgc agaaccaaac
    13561 cgatatttct gcgcagcgag gcttcgttga tcgttttcaa ttcggtccca tcaatcaaaa
    13621 tcgacccgcg ttggtaatcg tagaaccggc agaggagtga ggcaatcgac gtcttccccg
    13681 cgccggtggc gcccaccagc gcaatctttt cccccggttt gacggaaaaa ctcacttccc
    13741 ggaggacgta ctcctcgccc cggtaggcaa accagatatt tttgaattca atggcccctt
    13801 tgaccggagc cagctccttc gcgcccggtt tggcgctgaa ttcgggctgg gtatctaaca
    13861 gtttgaaaat ccgctccgag gacgccatcg ccgactggag gatgttgtac ttttcggcca
    13921 agtcgcggat cggctggtag aagcggtcca cgtactggat gaaggccacc agcgccccta
    13981 aggtgagaaa cccggagagc acctgccccc cgccgtacca gatgatgagg gcaatcgaga
    14041 gcgcgccgat caaatcgacc gccgggaaga agaccgcgtg atagaagatc gaacgcaggt
    14101 gggagtcgcg cagggaagcg ttgacgccgt cgaactggct gaaaactttc ttttcctgat
    14161 tgaacacctg caccgtggcc atgccggaga tctgctcctg cagaaaggcc ataatcttgg
    14221 ccaccttcag ccgcacttcg cggtaggcag cgcgtacctt tcgccggaaa accagcgtgg
    14281 catagaatag gagcggaatc gccacaaacg ccacgagagc cagcttccaa tttaagtaga
    14341 gcatgatggc gacaatgcct gcgagcgtga agagatcgcc gaaaaccgcc accacgccgg
    14401 aggtgaacat ttcgttcagc gcggcgacgt cggaagtgac gcgggtcaaa agccggccga
    14461 ccggatttcg gtcgtaaaac gagagggtca acttctgcag gtgggagaaa atccccatcc
    14521 gcaggtcgaa catcgccttc tgccccaccc actgcatcag gtaggtctgg acatagcgga
    14581 ccacgaactg cactgccaga atgaggatga agagcaaagc cagaccattc agcccgtgca
    14641 gattgccttt tttgatatag tcgtcaatgg caaccttggt caggtagggt ggggctaact
    14701 ggaggagcga tacggccaga agcagaacca cggcgattgc cacatggaag cggtacggct
    14761 tgaggtaggt caaaagccgc ttcatcagcc ggctgtcgta ggccttgcct aaaatgtctt
    14821 cttcgtggaa ctgttcagcc atagattatt ctatttcttc ttttccgtga ctttctcttt
    14881 tcgcgtaggt ggagcttcag ccccgcttca ccgttgcaag gcctacaatt ttttcagtcc
    14941 tccaccaatt gtctttcgag agacaccctt ccaagaaagt gtccaattga aaacgtatcc
    15001 attcaaccga ttttttagca atttcaacat ggtcagcgcc taagtcgatg tcttttgtta
    15061 tcttagtcgt aggccagaaa ttctctggaa agtcatcgat cattttgtgc actctcgacg
    15121 gacttagttc gatttcaatt tttctccgtt caggttttgc atgtacaaaa ttatctctaa
    15181 tgtcgactag ttcatcaaat atttgaaatt ctcttgcgcc cctgtcgaag aattttttgc
    15241 tccaatctac gggtgtgcag cacaaataag ggagtatcac aaccttgtca gttacagaac
    15301 accagctgac cccttttcgc ttatctatcc tgaacttgtt tatgatattc tctggaattt
    15361 ggtcgtctgg cacaactcga aagtcggagt tgagaatttc atttgccaat gcttcgactg
    15421 agaaaataaa catcaaaaaa gctgaacgag cgaacgtatg tgcctcttcg ataaatctct
    15481 gagcttgttt ttcctcttca cacagcgatt ttgaacgctc taaaagtctt ttcgcatctt
    15541 cgtaaaaaac ccaagatggc ttcgttacta aaatcccttc ctgaattgta ctcaccattg
    15601 tcctcctatc aaattagtcc cggatcgagt ttattgatct ggtacaacta atgacacatt
    15661 acaagatgga cattttgccc tttgacctgc tgaggttgaa gtgacggaca acagcgttcc
    15721 acacttccaa cagtaaaacg tcagttccct ctcattagcc actgactgct tgctccccgc
    15781 tccttcaggc tcttcatatg cttcagccgc atcctcctca actaaatcac gctcgggcca
    15841 ttccttaaga aggacatcac taggcaatcc ttcgcttgaa ctaaacatag ctggttgata
    15901 aatcgtttgc ttcttttttc tcttgaaggg agacagagtt accttaaata aagcccgcag
    15961 tgccttataa actaacacaa ccccaaaaat aataattagg acaatccatc ctgcgcctcc
    16021 aaaaaaaata gctaatagcc atcctactgc gttcaagatt ttctttagta tccaacgaaa
    16081 ccagaccttg gaacccgcac gttgaagaaa tgccacttta tacggttttg cca
//
LOCUS       DBNGZD010000264         5111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_665841, whole genome shotgun sequence.
ACCESSION   DBNGZD010000264 DBNGZD010000000
VERSION     DBNGZD010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_665841"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="AC16GM_07090"
     CDS             <1..599
                     /locus_tag="AC16GM_07090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR2923476.1"
                     /translation="FSWIATAWGGTIRFTTAMLFALGFVSLFVIGGMSGVILANIPVD
                     VQVHDTYFVVAHLHYVLFGGSLMALLGGLFFYFPKMTGRMLDEKLGKITFWLSYIGMN
                     VTFFPMHWLGILGMPRRISSYPDRPAFTFINQLETFGYVIILAGAILLLYNMWRSLRR
                     GAAASADPWQVNDIQKTLDWTVSSPPPVENFKEIPVVT"
     gene            596..1705
                     /locus_tag="AC16GM_07095"
     CDS             596..1705
                     /locus_tag="AC16GM_07095"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR2923477.1"
                     /translation="MSAEVVLTQPAAPSAGAEGEKIALARARIASWLVVLALVVFVLI
                     LWGGIVRLSGSGLSIPEWPIVNGSLLPPFSSGDWDAVYRTYHREILHITDLGSPEAIP
                     PRRFKAMFTIEYLHRFLASLTGILFLAIFVRAWRLKPVRSQVKGLLITAAFFLVAQAV
                     LGGIVVKRELEAEVVAGHLGLAFLFFATILWMALRLLRLPAAARVLPQSKLRLLAWSA
                     TGTVFLQILSGGLVAGTHAGWMMNTFPKMGDFLVPPLSVLWSSIYPTRWHNLVENQVL
                     LQFFHRWWAFVATLVVLFLIVRAIPFPLSPRGRLGLRAVGSVVVLQVLLGIGNLLMRV
                     PFWLSLVHLATALFLFGLLVLITHELRYRYETFPR"
     gene            1686..2561
                     /locus_tag="AC16GM_07100"
     CDS             1686..2561
                     /locus_tag="AC16GM_07100"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR2923478.1"
                     /translation="MKHSLANYLKLTKPEILLLVVLTGATALVMEGSLLAHPLRFGLV
                     ILALILTGGCANALNQYFERDLDSRMLRTRRKRPLPLGEISPRRALVFALALGAAALL
                     ILGMVFNLFSAVLALMTILFYSLFYTLWLKPRSHLNIVIGGAAGAMGPVIAWAAATGS
                     LSLTPWILFLIIFFWTPPHFWALALCVKEDYAKAKIPMLPVIKGDEETRRQIVVYTLW
                     LVGISLALLLVKAGAFSITLALALGGFFLWKAVQTFRRRTHRAAWGLFGYSILYLWVL
                     FIGMMADAVWHRAPH"
     gene            2580..3113
                     /locus_tag="AC16GM_07105"
     CDS             2580..3113
                     /locus_tag="AC16GM_07105"
                     /inference="COORDINATES: protein motif:HMM:NF016337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR2923479.1"
                     /translation="MNKKLKKNVATGLIFAGVAVGMFAFAFANIPVFRLYCQKVGIAL
                     SPNAKVKPGPAVSSNREVQILFTGVVAGGAPIFFSAQEAFMTVRLGKETETAYRFVNL
                     SADTIRFRPVHSILPEAAARRFSLKKCFCFDDQTLLPKQEVVLPVIFSINPDLDPEVE
                     QVTLNYTLFRKPLHGGN"
     gene            3113..3280
                     /locus_tag="AC16GM_07110"
     CDS             3113..3280
                     /locus_tag="AC16GM_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923480.1"
                     /translation="MSERKLEHSPWPGADVTGRLKRRNRWLGVFFLLFVLGIIALSLV
                     IFSKHGFPPHW"
     gene            3296..4102
                     /locus_tag="AC16GM_07115"
     CDS             3296..4102
                     /locus_tag="AC16GM_07115"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR2923481.1"
                     /translation="MADIAHTTTAVRHLAHAEHPEHGSPWPIILAGGLGLLAAGVLNL
                     IWQTKYGFPLLVTGGTIALLAVSNWAHTLIREKNFLENLFVDETWMKNGMKLFLISEA
                     AIFGAFFAHHYYARVHSPAWPPVGSPHLETRLPAIATLVLMASSGTCQWAHNLLRKGK
                     RVASQRGILLTMVLGILFLGAQGYEWGFLKAYDDFTLKNGAFGTSFFIMTGFHGLHVS
                     IGIILLSIVYVRLRLGHFTPERHFSMIAASWYWHFVDIVWIFLFGTMYLI"
     gene            complement(4200..4273)
                     /locus_tag="AC16GM_07120"
     tRNA            complement(4200..4273)
                     /locus_tag="AC16GM_07120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4237..4239),aa:Met,seq:cat)
     gene            complement(4337..>5111)
                     /locus_tag="AC16GM_07125"
     CDS             complement(4337..>5111)
                     /locus_tag="AC16GM_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2923482.1"
                     /translation="KLGSLDISYRCLRVLQRHRDVFWKNAKSNLIPPFFDPLFYLLAM
                     GYGLGALVAKIKGLSYPEFIAPAIAASVALTAPSFECIVGTLVRMKEQKTWDAMIHTP
                     VSVEDVITGEILFGAARSVIQAVSILIVIWLFGLIHSPLVVLLPLVIFISGIIHGSLA
                     MTYTSFFPEFNYMDYYFTLVMTPMFLFAGTFFTVERLPGWAHTVAWFMPLYHTTTISR
                     ALTTGLGSWQLVFNVLWLLVACALFYLLALVAMKRRVIK"
BASE COUNT          958 a         1495 c         1469 g         1189 t
ORIGIN      
        1 tcttctcctg gatcgccacc gcttggggcg gcacgatccg tttcaccacg gcgatgctct
       61 tcgctttggg tttcgtttcc cttttcgtta tcggcgggat gagcggggtg atcctcgcca
      121 acattccggt ggacgtgcag gtgcacgaca cctacttcgt ggtggcccac ctccactacg
      181 tcttgttcgg cggatcgctc atggcgctct tgggcggctt gttcttctac ttcccaaaga
      241 tgaccggccg gatgttagac gaaaagttgg ggaaaatcac cttctggctc tcctacattg
      301 ggatgaacgt caccttcttc cccatgcact ggctggggat tctgggcatg ccccggcgga
      361 tttcttccta ccccgaccgc ccggccttca ccttcattaa ccagctggag actttcggct
      421 acgtcatcat cctggcgggg gcaatccttc tgctctacaa tatgtggcgt tcgctcagac
      481 gaggggcggc ggcttctgcc gacccctggc aagtgaacga cattcagaag acgttggatt
      541 ggactgtctc cagcccaccg ccggtggaaa acttcaagga aattccggtg gtcacatgag
      601 cgcagaagtt gtgctgaccc aaccggccgc accgtcggct ggtgcggaag gagagaaaat
      661 tgctctcgcc cgggcgcgga tcgcctcgtg gctggtagtg ctggccttag tcgttttcgt
      721 tttgatcctg tggggaggga ttgtccgcct ctccgggtcg gggctctcaa tcccggagtg
      781 gcccatcgtc aacgggagtc tcctgccgcc cttttcttcc ggtgactggg atgcggttta
      841 ccgcacgtac catcgggaaa ttttgcacat taccgaccta gggtcgccgg aggccattcc
      901 cccgagacga ttcaaggcga tgttcaccat tgagtacctt caccggtttc tggcgagtct
      961 gacgggcatc ctctttttgg ccatcttcgt ccgggcttgg agattgaaac cggtgcgctc
     1021 ccaggtgaaa ggtcttttga tcaccgccgc attcttcttg gtggcacaag ccgtactggg
     1081 cggcatcgtg gtcaagcgcg aactcgaggc cgaggtcgtc gccgggcacc tgggtttagc
     1141 cttcctcttt ttcgccacca tcctgtggat ggctttgcgg cttttgcgcc tgcccgcagc
     1201 tgcccgggtg ctgccgcagt caaaactcag acttctggcc tggagcgcta ccggcacggt
     1261 tttcctgcaa atcctctccg gcggcctggt cgccggtacc cacgccggtt ggatgatgaa
     1321 taccttccca aaaatgggcg attttttggt gccgccgctt tcagttctct ggtcctccat
     1381 ctaccccacc cgctggcaca acttggttga gaatcaagtg ctcctccagt ttttccaccg
     1441 ctggtgggct ttcgtcgcca cgcttgtggt cctgtttttg attgtgcggg ccattccctt
     1501 tcccctttcc ccccgcggcc ggctggggct ccgtgccgtc ggctcggtgg tcgtactcca
     1561 ggtacttctg gggatcggga atctcttgat gcgggtgccc ttttggcttt ctctggttca
     1621 cctggccact gccctctttc tcttcggttt gttagtgctc atcacccacg aactgcgcta
     1681 ccgctatgaa acattccctc gctaattacc tcaaactgac caaaccggag attttactcc
     1741 tggtagttct gaccggtgcc actgcgctgg tgatggaggg aagtcttctc gcccacccgc
     1801 tgcgcttcgg gctggtgatt ttagctttaa ttctgaccgg cggctgcgcc aatgccttga
     1861 atcagtattt cgagcgcgac ctcgacagcc ggatgctacg cacccggcgg aagcggcctc
     1921 tgcccctggg ggaaatttca ccgcgccggg cgctcgtctt tgcactcgct ctgggagccg
     1981 cagcgctttt gattttggga atggtgttta atcttttcag cgccgttctg gcgttaatga
     2041 cgattctctt ttacagtctc ttctacaccc tctggctcaa accccgctcg cacctgaaca
     2101 ttgtgatcgg gggtgccgcc ggagccatgg ggccggtgat tgcctgggcc gccgccaccg
     2161 gctcgctatc gctcacaccc tggatccttt ttctcatcat cttcttttgg acaccacccc
     2221 acttctgggc tttagccctc tgtgtaaaag aagactacgc gaaagcgaag attcccatgt
     2281 tgccggtgat caaaggcgat gaggaaacca gacgccagat tgtggtctac accctctggc
     2341 tggttggaat cagtttggcg ttacttttag tgaaagccgg cgcattctcc ataaccctgg
     2401 ccttggctct ggggggcttt ttcctctgga aggcggtgca aaccttccgg cgccgcactc
     2461 accgggccgc ctggggactg ttcggctatt ctatcctcta cctgtgggtg ctttttatcg
     2521 ggatgatggc ggatgcggtc tggcaccgcg ctccgcattg aggagaagat gggaagacga
     2581 tgaataagaa attgaagaag aacgtagcga ccggactaat cttcgccggc gtggcggtgg
     2641 ggatgttcgc ctttgccttt gccaatatcc cggtcttccg gctctactgc caaaaagtcg
     2701 gcatcgccct ttcgcccaac gccaaggtta agcccggtcc ggcggtttcc agcaaccgcg
     2761 aagttcagat tctcttcacc ggcgtggtgg ccggcggcgc acccatcttc ttctcggccc
     2821 aggaagcgtt tatgaccgtc cggctgggaa aagagaccga aaccgcgtac cggtttgtga
     2881 atctttcagc cgacacgatc cgcttccgtc cggtgcacag catcctgccg gaggcggcgg
     2941 ccaggcgctt ttcactcaag aagtgcttct gcttcgacga ccagacgcta ttgcccaagc
     3001 aggaggtcgt gctgccggtc atcttttcca tcaatcctga tcttgatccg gaggtggagc
     3061 aggtgacctt aaactacact ctgtttcgga aaccgctcca cggggggaat tgatgtcgga
     3121 aaggaagctt gagcattcgc cgtggccggg cgccgatgta acgggccgtc tcaagcggag
     3181 aaaccgctgg ctgggagttt tcttcctgct gttcgttctg ggaattattg cactgagttt
     3241 ggtgatcttt agcaaacacg gcttcccgcc gcactggtga taaagagagg gagcaatggc
     3301 tgatattgca catacaacaa ccgcggtgcg ccacctggcg cacgccgaac acccggagca
     3361 cggcagcccc tggcccatta tcctagccgg agggctgggg cttctggctg ccggggtgct
     3421 gaacctgatc tggcagacta aatacggttt tcccttgctg gtgacgggcg gaaccatcgc
     3481 ccttctggcg gtcagcaact gggcgcacac cctcatccgg gaaaagaatt ttctggaaaa
     3541 tttattcgtc gatgagacct ggatgaaaaa cgggatgaaa cttttcttaa tctccgaagc
     3601 ggccattttc ggcgccttct ttgcccacca ctactacgcc cgggtgcact ccccggcctg
     3661 gccgccggtc ggatcgccgc atttggaaac ccggctgccg gccattgcca cgctggtttt
     3721 gatggcctcc agcggcacct gccagtgggc gcacaacctg ctgcggaaag ggaaacgcgt
     3781 ggccagccag cgcggcatcc tgctcacgat ggttttgggt attctcttct taggcgccca
     3841 ggggtacgag tggggcttcc tcaaggccta cgatgatttt accctcaaga acggagcttt
     3901 cggcaccagt ttcttcatta tgaccggctt ccacggcctg catgtgagca tcgggatcat
     3961 cctgctttct atcgtctacg tccggctgcg cctgggccac tttacgccgg agcgccactt
     4021 ttcgatgatc gccgcttcct ggtactggca cttcgtcgac atcgtctgga ttttcctctt
     4081 cgggacgatg tacttaatct gagcccaggc agtacgcaga aattatcagg cccgctcaat
     4141 caaagcgggc cttttctttg gcttcctgga ggttaaagtg agccgggata atgagggaat
     4201 agcggcgagt ggattcgaac caccgacacg cggcttatga agccgctgct ctaaccgact
     4261 gagctacgcc gccacagagg gtagaatccc atcaaaagaa aagccaaaaa aagaaaactg
     4321 gcaagggagc tggccgttac ttaatcaccc ggcgtttcat tgccacgagg gccaggaggt
     4381 aaaagagagc gcacgcaacc agaagccaga ggacgttgaa caccagctgc cacgatccca
     4441 aaccggtggt gagggcacgg gagatggtgg tggtgtggta taaaggcata aaccaggcca
     4501 cggtgtgcgc ccagcccggg agccgctcca ccgtgaaaaa ggtcccggcg aacaggaaca
     4561 tcggggtcat caccagggtg aaatagtagt ccatgtagtt gaattccggg aaaaaggagg
     4621 tgtaggtcat cgccagggag ccgtgaatga tgccggatat gaaaatgacg agggggagaa
     4681 gcaccaccag aggcgagtgg atgagaccga agagccagat gacgattaaa atcgacactg
     4741 cctggatgac gctgcgcgcc gcgccgaaga ggatttcgcc ggtgatgacg tcttccaccg
     4801 ataccggcgt atggatcatg gcgtcccagg ttttctgctc cttcatccgc acgagcgtcc
     4861 cgacgatgca ctcaaacgat ggcgccgtaa gcgccaccga ggcggcaatc gccggtgcga
     4921 tgaactccgg gtaggagagc cctttgattt tggccaccag cgcccccagg ccgtacccca
     4981 tcgccagaag gtagaagagc ggatcgaaga agggcgggat gaggttgctc ttggcgtttt
     5041 tccagaagac gtcccggtgc ctttggagca cccgcaggca acggtaggag atgtccagtg
     5101 agccaagttt c
//
LOCUS       DBNGZD010000265         1821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_736968, whole genome shotgun sequence.
ACCESSION   DBNGZD010000265 DBNGZD010000000
VERSION     DBNGZD010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_736968"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="AC16GM_07130"
     CDS             <1..1256
                     /locus_tag="AC16GM_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR2923483.1"
                     /translation="TALGRDITLESLQIHFDAVFLAFGTQESKRLKLEGRELKGVWWG
                     LDFLRRTKSGEKMALGDKVVVLGGGDVAIDAAMTALRLSAESVEIFCLEKKEELPAHP
                     WQLRQALEEGVVLNCSWGPRRISGDGNNVTGVELVKCISVFDSNGNFKPQFDESQRLS
                     HDASTVIFAIGQTLDSSIFDDGASRTLIQDGRVKANLSTLETNLKGIFAGGDAVFGPA
                     SVVEAIRAGRMAAQSIDRYLGGNGELGMPCEVSGARPSRELGGWLGRKEGFAHWGRAS
                     APTLSADQRKRAFAEIESTYGRLQGVEEADRCLRCHLRLAIPKVLLPPEELLPFNPEQ
                     VESVAESEGVLQLLNQEKKIVLIAGTPALRSRLREELKSHKNARFFVFEESKMYTQRE
                     TELLQRFLQQNGKLPEGNDELGDLL"
     gene            complement(1268..>1821)
                     /locus_tag="AC16GM_07135"
     CDS             complement(1268..>1821)
                     /locus_tag="AC16GM_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR2923484.1"
                     /translation="KVDEMIKTFHRIFLNLDGLKISTLSVVQGTALGGGCELAGFCDI
                     VLASEAAKFGQPEIKVGVFPPVALAYFANFMAPKKVLELAVTGEIKTAREALEMGLVT
                     KVCPADKLEADAGIFVDKIKGLSAAVLRLAKKSWKECIRIKFGEALGKTEQIYLDELM
                     ATRDANEGLAAFLEKRAPVWENR"
BASE COUNT          433 a          455 c          496 g          437 t
ORIGIN      
        1 atactgctct ggggcgcgac ataactctgg aaagtttaca gatccatttt gacgcggtct
       61 tcttggcttt tggcacccag gaaagcaaac ggttgaaact cgaaggaagg gaattaaaag
      121 gggtctggtg ggggctggat tttttgagaa ggacaaaatc tggtgaaaaa atggcgcttg
      181 gggacaaagt ggtggtactt ggcggggggg acgtagcgat cgatgcggca atgaccgctt
      241 tgcggctttc tgccgagagc gtggaaatct tttgtttgga aaaaaaagaa gagcttcccg
      301 cccatccgtg gcagctccgg caggccttgg aggaaggagt cgtgctcaat tgctcctggg
      361 gacccaggcg aatttcggga gatggcaaca atgtgacggg tgttgagctg gtgaaatgca
      421 tttccgtttt tgattcaaac ggcaacttca aaccccaatt tgacgagtcg caacggctct
      481 cacatgatgc cagcaccgtg atctttgcaa ttggtcaaac cctggattcc tctattttcg
      541 atgacggtgc ttccagaact ctgattcagg atggtagagt aaaagccaat ttgagcactt
      601 tggaaacaaa tctgaaagga atctttgccg gcggcgacgc ggtctttggc ccggcttccg
      661 tggttgaggc catccgggca ggaaggatgg cggctcaatc gattgaccgg tacttgggtg
      721 gaaatggaga attgggaatg ccgtgcgaag tatccggcgc tcgcccatcc cgcgaactgg
      781 gtggttggtt ggggaggaaa gagggttttg ctcattgggg gcgggcttcc gctccgaccc
      841 tgtcagccga ccaaagaaaa agagctttcg cagagattga atcaacttac ggccggctgc
      901 agggagtcga ggaagccgac cgctgcctgc gctgccatct gcggctggcc attcccaaag
      961 tccttctccc gcccgaggag ctcttgccct ttaatccaga acaagtcgag tccgtagccg
     1021 aaagcgaagg ggtgctccaa ctcctgaatc aagaaaaaaa aattgttcta atcgctggaa
     1081 cgcccgccct tcggagccgg ttgcgggagg aactgaagag ccacaaaaac gcccgctttt
     1141 ttgtttttga agaaagcaag atgtacaccc agcgggaaac cgagctttta cagcggttct
     1201 tgcagcaaaa cggaaagctt ccagagggaa acgacgaact gggggatctt ttgtgagaaa
     1261 gaattcttta ccggttctcc caaaccggtg ctcttttttc caaaaatgcg gccagtcctt
     1321 cattggcgtc ccgggtggcc atcagctcat ccaaataaat ttgctcggtt tttcccaaag
     1381 cttccccgaa ttttatccta atacactctt tccagctttt cttggcaagc cggagaacag
     1441 ccgcgcttag gcctttgatt ttatcgacga aaatacctgc gtccgcttcc agtttgtctg
     1501 ccgggcagac tttggttacc agacccattt ccaatgcttc tcgggcagtt tttatctctc
     1561 ccgttacggc cagttccagc actttcttgg gagccataaa attcgcaaaa taagccagtg
     1621 cgacaggcgg gaaaacccct accttgattt ccggctgtcc aaattttgcc gcttccgagg
     1681 ccagcacgat gtcgcagaag ccggccagtt cgcagccacc accaagggcg gttccttgga
     1741 ctaccgacaa agtggaaatt ttcagcccgt ccaggttcaa aaagatccgg tggaacgttt
     1801 ttatcatctc atccacttta t
//
LOCUS       DBNGZD010000266         5776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1292530, whole genome shotgun sequence.
ACCESSION   DBNGZD010000266 DBNGZD010000000
VERSION     DBNGZD010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1292530"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..894
                     /gene="ccmA"
                     /locus_tag="AC16GM_07140"
     CDS             280..894
                     /gene="ccmA"
                     /locus_tag="AC16GM_07140"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR2923485.1"
                     /translation="MTLSFSGLSKSFGFRVVFENISTTVAHGSVLVVTGPNGSGKTTL
                     LRIIAGLLRPSAGRITLALDSGEIPKEELRRHLALVAPDLQLYDELTALENLAFLTRL
                     RGFKHPQAHLEMALQEVGLAERGGDLVKAYSSGMKQRLQYAYARLLEPTLLLLDEPTA
                     NLDDAGKQMAHAFIQRQKERGIVVIATNEKEETRYGDQVIRLGR"
     gene            863..1546
                     /locus_tag="AC16GM_07145"
     CDS             863..1546
                     /locus_tag="AC16GM_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR2923486.1"
                     /translation="MATRSSGWAGNARAVLAKDLRSELRTRYALNTILLFGLVTVVAE
                     SFAIGQVRLEPDVLAALLWIAIFFAAMSGLAQVFVKEEEARTANTLKLAAAPEAVFLG
                     KLGFNFLLLLGLEIIILPLFFLLFNPALGNLPLFLVVTFLGTCGLVAATTIIAAIIAK
                     ASVKGALFAALSFPIVLPLIIVSITATRIALEGGSFAEGWDYIRMLIAYLGVMLVGSY
                     LLFEFVWYE"
     gene            1664..1736
                     /locus_tag="AC16GM_07150"
     tRNA            1664..1736
                     /locus_tag="AC16GM_07150"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1697..1699,aa:Thr,seq:cgt)
     gene            1965..2522
                     /locus_tag="AC16GM_07155"
     CDS             1965..2522
                     /locus_tag="AC16GM_07155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR2923487.1"
                     /translation="MPFQFKVFLFIVASVGIAWVSRASVRDPHSHGFYRFFAWEAILV
                     MILLNLNYWFYAPFSIHQIASWVLLIVSTFLVIHGARLLRLVGKPASQRNDPALIGIE
                     KTTELVTVGAYRYIRHPIYSSLLFLAWGVFFKHPSWVGVSLAVITIFFLTMTAKMEEA
                     ENLRFFGATYQRYMKQTKMFIPFLF"
     gene            2740..4101
                     /locus_tag="AC16GM_07160"
     CDS             2740..4101
                     /locus_tag="AC16GM_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2923488.1"
                     /translation="MPAVLVIDDKDSMRNMLSQTLVADGFEVETVPSGPQGIERAREK
                     RFDVVLTDLKLPDMDGLQVLIKLKEIDPDLSVIVMTAYGTIETAVQAMKEGAYDFLTK
                     PFDTDHLGVLIRRALENRRLLAENILLREELAHSLGYAEIIGKSEKMVEVSHLVQKVA
                     PSDTTVLLLGESGTGKELFARAIHNLSLRKNAPYVAINCAAIPSELLENELFGSEKGA
                     FTGAWARKMGKFEIAEGGTIFLDEVGDMSVALQAKLLRVLQEKTFERLGGNKTLSVDV
                     RVIAASNADLKAAIDKKQFREDLFYRLSVFPLAVPPLRERREDIPLLAEHFIAKYCAE
                     MKKPKKTLSSETLSVLDRYHWPGNVRELENTIERAVILCEGKRITPEHLAIRIPSVQE
                     IRLREGAGLKEVGAHAQMEAERALIQRVLNQTRGNKRKTAEILQIDYTTLFDKMKKYA
                     IEF"
     gene            4336..4722
                     /locus_tag="AC16GM_07165"
     CDS             4336..4722
                     /locus_tag="AC16GM_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR2923489.1"
                     /translation="MLRLKNKRGFSLVEVLSAMVVLAIGILGLAPLIVLSIRTNSYSQ
                     DVTDASVFAQDRIEQLKSQASFTPMPYTETTNPGGGLTRTVRIDNNATDGTCPAGMYR
                     IAVTITWTEQNNVTRRVVFNTYKPVS"
     gene            4732..5004
                     /locus_tag="AC16GM_07170"
     CDS             4732..5004
                     /locus_tag="AC16GM_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923490.1"
                     /translation="MRWVVGVIWILSESFLATLGRGEKTSWRTEMTITENREMQDKPG
                     ERSQKLDSVRSWPPHAGRLVKKAAPLPGFRPNFFQMVTRFRTGERV"
     gene            5331..>5776
                     /locus_tag="AC16GM_07175"
     CDS             5331..>5776
                     /locus_tag="AC16GM_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR2923491.1"
                     /translation="MKKPKLFHWAQKWTEIYRSQRGFSIIEMLIVGFLTALLAGFTLE
                     FYLTQHNQWLTQTQLADLQQNGRNSMDALTGTIRSAGYGVLFGAPAFQVNTNQDTLAI
                     YAQNGAATDTTRFFVDRSIANNPRLLRKINSLESQVCAEYVDTVGFV"
BASE COUNT         1262 a         1597 c         1642 g         1275 t
ORIGIN      
        1 gcgctgggag gaaaacatgg atttcatgtt gaccctttgg gccccaatct ctcctgtgca
       61 acatgtcatg accaagtagt tactgaagcc attccgccaa caattccttc cattcgtaca
      121 cttgcccttc acgttaatgg cctatacgac attgccttta atagtggcgg tcgagggtac
      181 atctgggatc cggcggccaa gacctgcgga gggtcgaata ctcactgtca ccgcaatccc
      241 cccacggacc ttcgtaactg gtatgcaccg tagggtatgg tgaccctttc cttttccggt
      301 ctttccaaaa gtttcggatt ccgcgtcgtt ttcgaaaata tctcaaccac cgttgcacac
      361 ggttctgtgt tagtggtcac cgggcccaac ggttcgggca agacgacact tctgcgcatt
      421 atcgccgggt tgctgcggcc ctctgcaggc cggatcacgc tcgcccttga ttcgggtgaa
      481 attcccaaag aggaactccg ccgccatctg gcgttggtcg ctcccgattt gcagttgtac
      541 gacgaattga ctgcgctgga aaatctcgcc ttcctcacgc ggctgcgcgg gttcaaacat
      601 ccccaggcgc atctcgaaat ggcgttacag gaagtcggcc tggccgagcg cggcggcgat
      661 ctggtgaagg cctactcctc cgggatgaag cagagactcc agtacgctta cgcccgcttg
      721 ctggaaccga cactcctgtt gctcgacgag ccgacggcga atttagatga tgccggcaaa
      781 caaatggccc acgcgttcat ccagcggcaa aaagagcgcg gcatcgtggt gattgccacc
      841 aatgaaaagg aagaaacccg ctatggcgac caggtcatcc ggctgggccg gtaacgcccg
      901 ggcggttttg gcgaaagacc tccgctcgga actgcgtacc cgctacgcgc tcaataccat
      961 cctcctgttt ggcctggtga cggtggtggc ggagagtttc gccatcggtc aagttcgcct
     1021 ggagccggat gtcctggcgg cgcttttgtg gattgccatt ttctttgcgg cgatgtcggg
     1081 gctggcgcag gtattcgtca aagaagaaga ggcccgcacg gcgaatacgc tcaaattagc
     1141 ggctgcgccg gaggcggtgt tcctcgggaa attgggtttc aatttccttt tgcttttggg
     1201 tctggaaatc atcatcctgc ctttattctt tttgctcttc aatccggcgt tgggcaacct
     1261 gccgctcttt ctggtcgtca catttctggg cacctgcggg ctggtggcgg ccacgacgat
     1321 catcgccgcc atcatcgcca aggcgtcggt caaaggggcg ctcttcgccg cgctgtcatt
     1381 tccgattgtg ctgcctttga tcatcgtctc catcaccgcc acccggatcg ccttggaagg
     1441 cgggagcttc gccgagggct gggactacat ccggatgctc atcgcctacc tcggggtgat
     1501 gctggtcggg tcgtatttgc tgtttgaatt cgtgtggtac gagtgaccgg aggaaaatga
     1561 tatagccgtg aagttctggt ctccttgacc ggctcaggga cgagccggtc taccggtgac
     1621 ggggcttgac aggtcctctc atgtctttac cttgactttt cacgccacgg tagctcagct
     1681 ggtagagcag cggtttcgta aaccgcaggt caacggttcg aatccgttcc gtggcttttt
     1741 caaagagttt cccttcaata atcgcagacc gagacgtttc attcgaggag ctttgacctg
     1801 gccattccag ccagccaaag agaacatgtt cctgaagcgg tgggccactc actcagctcg
     1861 acatagccgc tcagtcgacc tgatgtcggc tcagccgttg gccggcatct tgcttactct
     1921 tccaagcgta aaaataaata gtttgctata ttcggattgc cagcatgcct ttccaattca
     1981 aggtgtttct ttttatagtt gcatctgtag gaattgcctg ggtgtcacgg gcatccgtac
     2041 gtgatccgca ctctcacggc ttctaccgct ttttcgcctg ggaagcgatt ttggttatga
     2101 ttctgttaaa cctcaattat tggttttatg caccgttcag tatccatcag atagcttctt
     2161 gggtcttgct aatagtctcc acttttttgg taattcacgg tgctcgatta ctaaggttag
     2221 tggggaaacc ggccagccag cggaacgacc cagcgttgat tggtatagaa aagaccactg
     2281 aattggtgac ggtgggagct tatcgctaca ttcgtcatcc catctatagt tcgttactgt
     2341 ttctggcctg gggagtgttt ttcaaacatc cttcctgggt tggtgtttct ttggctgtta
     2401 ttacgatttt ctttctcaca atgaccgcta agatggagga agcggagaac ttacgttttt
     2461 ttggtgccac ctatcagagg tatatgaaac agacgaagat gtttatccct tttttattct
     2521 aagaaggttc gggggtaccc ggggcaatgc catcaccggg tggaagtccg cgggacctca
     2581 aaaaagcatg gttcgcaaat cataggaacg aaaattcccc ctattgaacg cccgctatcc
     2641 tcctttccgg ttgccccaca cttcacagat tgccaaatcc gccgataata caatcaggaa
     2701 attcgatcaa aattttttca tagttgcgga ggatactgga tgccggccgt actggtcata
     2761 gacgataaag acagcatgcg caacatgctc tcccagacgc tcgtggcgga tgggtttgaa
     2821 gtggaaaccg tgccctccgg cccgcaggga atcgagcgcg ccagagagaa acgcttcgac
     2881 gtcgttctga ccgacctgaa gctgcccgac atggacgggc tgcaggtgtt gatcaaattg
     2941 aaagaaatcg accccgacct ctcggtcatc gtcatgaccg cctacggcac catcgagacg
     3001 gcggtgcagg cgatgaaaga gggggcctac gattttctga ccaagccgtt cgacaccgac
     3061 cacttgggcg ttctgatccg gcgggctctg gaaaatcgcc gccttttggc cgagaacatt
     3121 ctcctgcggg aagaactggc ccactcgctg gggtatgcgg agatcatcgg caagtcggaa
     3181 aaaatggtcg aagtctcgca cctggtgcag aaagtggcgc ccagcgatac gaccgtgctg
     3241 cttttaggcg agagcggcac gggcaaggaa ctcttcgccc gggccatcca caacctctcg
     3301 ctccgcaaaa atgcacccta cgtggccatc aactgcgcgg cgatcccgtc ggaacttttg
     3361 gaaaacgaac tcttcggttc agagaaaggg gccttcaccg gggcctgggc gcgcaaaatg
     3421 gggaagtttg aaatcgccga aggcgggaca attttcttgg acgaagtggg ggatatgagc
     3481 gtcgccctgc aggcgaaact cctccgcgtc ctccaggaga aaaccttcga gcggctgggc
     3541 gggaacaaga ctttgtccgt ggacgtgcgg gtcatcgccg cctccaatgc cgatctcaaa
     3601 gccgccatcg ataagaagca gttccgcgaa gacctgttct atcgcctgtc ggttttcccg
     3661 ctcgccgttc cccctttgcg tgaacggcgc gaggacatcc cccttctggc cgagcacttc
     3721 atcgccaaat actgcgccga aatgaaaaag cccaagaaaa ccctctcgtc ggagaccctc
     3781 tcggttctgg accgctacca ctggccgggg aatgtccgcg aactggaaaa caccatcgag
     3841 cgggcggtga tcctctgcga agggaaacgg atcacgcccg agcacctggc gatccggatt
     3901 ccctcggtgc aggagatccg gctgcgggag ggggcggggc tcaaagaagt gggggcgcac
     3961 gcgcagatgg aggcggagcg ggccttaatc cagcgggtgc tcaaccagac ccgcggcaac
     4021 aagcgcaaaa ccgctgaaat tctccagatc gactacacga cgctcttcga caaaatgaag
     4081 aagtacgcga tcgagtttta gtccacgctc gctgtgggat tttccccaca ctttattgcg
     4141 cgtcaactca aaatttgatg ggcaagagct gacctgtcgg tttgtccgct ctggtcggca
     4201 ccggtaaccc ttttgaggga aaggggttgt tgctggccgg gtcggttcgt gcaataaccg
     4261 ggaatacggc aatccatttg caattggtca gagcgtgagc cagaacagaa tagggattac
     4321 gatggaggag tgcgaatgct aagattaaag aacaaacgcg ggttttcact ggtcgaagtg
     4381 ctctcggcaa tggtggtact ggccatcggc attttgggtc tggcaccgct catcgtgctc
     4441 tcgatccgga ccaactccta ttcccaggac gtgaccgatg cgtcggtttt tgcccaggac
     4501 cggatcgagc agttaaaaag ccaggccagt ttcacgccca tgccgtacac cgagaccacc
     4561 aatccgggtg gcgggctgac gcgcaccgtg cggatcgaca acaacgccac cgacggaacc
     4621 tgcccggccg gcatgtaccg catcgcggtc accatcacct ggaccgaaca gaacaacgtg
     4681 acccggcggg tggtctttaa cacctataag ccggtgtcgt aagggtgcgc catgcgctgg
     4741 gtggtgggag tgatctggat cttaagtgaa agcttcctgg ccaccttggg caggggagaa
     4801 aagacgagtt ggcgaacgga aatgactatt accgagaaca gagaaatgca agacaaaccg
     4861 ggagaacgct cccagaaact tgattccgtc cggtcgtggc caccccacgc ggggcggctg
     4921 gtgaaaaaag cggcacccct gccgggcttc cggcccaact tcttccagat ggtgacgcgc
     4981 ttccgaacgg gcgagcgggt gtaaggagca aaaagacgat ctttcagttc gtgaggaaaa
     5041 cgatgcgcaa gagcggcaca aaaacgagca ggaaagagag cgtgcacgtg agcagggtga
     5101 ccgtcccaaa ccagaagcgg aatactaatg gaacggccgc cggactcccg gccgagagta
     5161 gggggagaac ccccgctcgt tggcgccgac cgctggggag cgaaccagat ttgccctggg
     5221 gacaggttcc tttcttctat gctgatttca acttaggcag aaagccagga aagagtattc
     5281 gccggcatga tggttgcaat ccggtgctgg ccagacgagg agacaagcca atgaagaaac
     5341 caaaactatt ccactgggcg cagaagtgga cggagattta cagaagccag cgcggtttct
     5401 cgattattga aatgttgatt gtcgggttct tgaccgcgct tttggccggc ttcaccctgg
     5461 agttctacct gacccagcac aaccagtggc tgacccagac ccagctggcc gatctgcagc
     5521 agaacggccg caacagcatg gacgcactga ccggcaccat ccgcagcgcc ggctacggcg
     5581 tgctcttcgg tgcgccggcg ttccaggtta ataccaacca ggatacgctg gcgatttatg
     5641 cccagaatgg agcggcgacc gataccacgc gctttttcgt ggaccgctcc atcgcaaata
     5701 atccccggct cctgcgcaag ataaacagtc tggaaagtca ggtgtgcgcg gagtacgtcg
     5761 acaccgtggg ctttgt
//
LOCUS       DBNGZD010000267         6903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_529923, whole genome shotgun sequence.
ACCESSION   DBNGZD010000267 DBNGZD010000000
VERSION     DBNGZD010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_529923"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="AC16GM_07180"
     CDS             complement(<1..1304)
                     /locus_tag="AC16GM_07180"
                     /inference="COORDINATES: protein motif:HMM:NF040218.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HIR2923492.1"
                     /translation="MRFAKFFFVLAGLTIFLSSATARADSVLVVTPPSLPPFTMAEGG
                     PNPAPQKLRIRNCCPGELRFGVFTNPSAWLKVNGQPAVINLTTPDSVDISVEATTLPP
                     GVYTGEVTVSSPLTFPVTVPCTLTVNAAPQPVIDVSPPSFFFTTQQGTNPPAQQLIVT
                     NGGTGTLIYSLFVSGTWLRADGSPTGFQNHTAPDTTTISVNTQGLDTGTYIGQLTLTA
                     SGVTPATVPVTLTVTAPPQPLIHVNPASFSFTAEQGTNPPSQNLAVTNGGAGTLVYSL
                     FVTASPSGWLLANGSPSGFLNHTAPDNVTISVNTGGLPPGLHVGQLTLTASDAPTVNV
                     PCTLTVTFNPPIIDVAPDSFIFTAMQGGPDPAPQVLSVTKGSAVNFNFNAFKTATWLL
                     VNPSVNVPAPAFLTVSAQVGSLPVGDYRDVIDITATGAQTKHV"
     gene            1776..2171
                     /locus_tag="AC16GM_07185"
     CDS             1776..2171
                     /locus_tag="AC16GM_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2923493.1"
                     /translation="MSLEKHKVLVVDDELLIRDLLYDFFSGRECEIAVAASGQEALKQ
                     LEQHSFDVILVDLKMPDMDGLDLARAIRASGHTSPIILITGYPSVESAVEALRIRLYD
                     YVTKPFNINQLFHLVSRAAREYREQGEKV"
     gene            2172..2687
                     /locus_tag="AC16GM_07190"
     CDS             2172..2687
                     /locus_tag="AC16GM_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2923494.1"
                     /translation="MSAPETPHQSAVPQILVIEDELYLRETLADYLGQQGYRVRTCES
                     GEQGVLLHQEERSDVALIDLWLPGRDGLEITRLLLEQDASLRIVLMTCRPNLVSATDA
                     LREQVFDYLIKPFRLPEVRDVVERAVRHAGLCIPAPPQVEPFGPPGPDEYPASGGTLR
                     QFPPLVWRFVF"
     gene            2797..3648
                     /locus_tag="AC16GM_07195"
     CDS             2797..3648
                     /locus_tag="AC16GM_07195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR2923495.1"
                     /translation="MNQKQQTVPFKTTVLAPLDTSSAAPAEDVTVLQQAYRELEQRCR
                     SLTAELDRLKPLAALGERIALVAHELRNPLGGIAGFAAILDHDLPENDPRKPLIGKIL
                     AGIEQLNRMAEGLLHRARGPEIRPSRFDAVQFLEDITVQCETELRSAGRRVILRRDFP
                     EKTLPVDLDPEYFRQAVTNLLANSFQAVGEEGEVRVSANLEPSEPPTPRKTLVVKVQD
                     NGPGMTPELLQKIFTPFFTTKTDGSGLGLAIVRQAAQAHGGEVAVTSAPGKGATFTLR
                     IPQDVQR"
     gene            3645..5009
                     /locus_tag="AC16GM_07200"
     CDS             3645..5009
                     /locus_tag="AC16GM_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2923496.1"
                     /translation="MNPRARILVVDDEPLIREFLNETLSRSGFLVEQAAAGEEAWQKI
                     QSASGGYDVVFTDVKMPQMNGLELLRTVKAHNPDVSVVVLTGYGSIADAVAAIKLGAF
                     DYLTKPCTPAQIEHAVQKALEFQRLVAENRQLRQELVSRYQYANIVGKSALMQKVFAL
                     VDTVASSRATVLITGESGTGKELVAHAIHYNSPRREGPFVKLNCAALPEGLIESELFG
                     HERGAFTGAIRSARGRFERADGGTLLLDEISEMPLGLQAKLLRVLQEREFERVGSGDT
                     IQVDVRIVATTNRNLSAVIREGKFREDLYFRLNVVNIPIPPLRDRLEDLPYLVNHFIR
                     KYNEENGKKIEGISEKVLAVFSRYHWPGNVRELENYLERAVVLSPGPLLQPSDFPPEV
                     TLGKITAPTDGVKIGMSLHDTEKLMILKTLESHAGNRTKAAEVLGISTRTLRNKLEEY
                     GLKE"
     gene            complement(5076..>6903)
                     /locus_tag="AC16GM_07205"
     CDS             complement(5076..>6903)
                     /locus_tag="AC16GM_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2923497.1"
                     /translation="KDRAAVAAMMNDAQAIVPNPVRYPAGFEHGVIAVGASTDADDRA
                     QFSNTGRHIDVVAPGGIDFNGGNPKNILCPTWPGNNAYIFETGTSFAVPHVAGLAALL
                     KGYDNFLSNDDIQNLIQKTSDDRGPAGWDSSFGYGRINARRALEKANCLVVQHYTAAG
                     GTVSGSTDTLRDFKFFTVIGIPNDGARFKVILDTVTTTVAFDTSLGLVDTVWGRGVGT
                     KGFSTESPNHGMGFCDVKTFSSTGATLRTYVYRVFNPDGTLKGTFPAQPQDVVFAYTV
                     LRKTGPVVTANDNSVPDRTILTWPDGWSCEDGFKIYRSPNAGSTWDSTAQVASNVTTF
                     TDSTRLGSKTYKWRVSPFKGVAVDTGQPSIQVTTKPIRPQNFQASVTTFCFGFGSGMP
                     EAMVTDTGQGLSLPCELVGTNQVVVTWQRPTNQNPLDSIKEYVIRARRAPSFEVEQEH
                     VLSASRKADTLNCLELNTLYRIEGHSRDQTNDSSVVTGQQVVTGPRQLCAGSDPKPVT
                     PPALQSQTFSWSLFQNHPNPFNPETDIRYSLAQEAQVSLAIYNLLGQKVRELVNAQQP
                     AGIYTAHWDGKDELGRPVGSGIYFARLVVGEFVDTKRMLLVK"
BASE COUNT         1453 a         1936 c         2083 g         1431 t
ORIGIN      
        1 acgtgtttgg tttgtgcgcc ggtggcggtt atgtcaatga cgtcacggta gtcacccacc
       61 ggcagtgagc cgacctgagc cgagacggtg agaaaagcgg gcgccggcac gttcacgctg
      121 ggattcacca aaagccaggt ggcggtcttg aaagcgttga agttgaaatt caccgcactg
      181 cccttggtca cacttaacac ttgcggggcg ggatcgggtc cgccctgcat ggcggtgaag
      241 atgaacgaat cgggggcgac gtcgattatc ggcgggttga aggtaactgt gagcgtgcag
      301 ggaacattaa ctgttggtgc atcactggcg gtaagagtca attggccgac gtgcaggccg
      361 gggggaagtc ccccggtgtt gaccgatatc gtcacgttat ccggggcggt atgattcaga
      421 aaaccagaag gggagccatt ggcgaggagc cagcctgagg gactggccgt cacgaagagg
      481 ctgtatacca gcgttcccgc gccaccattt gtcaccgcta agttctggga gggagggttg
      541 gtcccctgct cggcagtgaa ggaaaaactg gccgggttga cgtgaatcag gggctgggga
      601 ggcgcagtga ccgtcagcgt gaccggaacc gtagcgggcg taactccgct ggcggtgagg
      661 gtcagctggc cgatgtacgt accggtgtcc agaccctggg tgttgacgga aatggttgta
      721 gtatccgggg cggtgtgatt ttgaaagccg gtaggagaac catcggcccg gagccaggtg
      781 ccggagacaa agaggctgta gatcaaggtt cccgtcccgc cgttcgtcac gatcaactgc
      841 tgagccggag gattggtgcc ctgctgggtg gtgaaaaaga aactgggcgg actgacatcg
      901 atgaccggct ggggagcggc gttaacggtt aaggtgcagg gcaccgtaac gggaaatgtg
      961 aggggggacg aaaccgtaac ttctcctgtg tacactcccg gaggcagagt agtagcctcg
     1021 accgaaatat ccacggagtc gggagtggta agattaatta ctgccggctg gccgttcact
     1081 tttagccagg cactaggatt tgtaaaaact ccaaatctca gttccccggg acagcagttt
     1141 ctgatgcgca gtttctgcgg cgcaggattc ggcccaccct cggccattgt aaacgggggg
     1201 agacttggcg gcgtgaccac cagcaccgaa tcggcccgcg cggtggcact cgagagaaaa
     1261 atcgtcaagc ccgccagtac gaaaaagaat tttgcgaaac gcatcccgat cctcctcaaa
     1321 gcaaactcac ttcctctgcc gtgctcgcgc acgaaccgac accaaaagac aaagactcct
     1381 caaacgccaa ggttactcct tgcgggcagc ctccctcagg ttgcacccga aatataggac
     1441 taaccggcat actgtcaagc gataatagag catatcctgt gccgtcaata ttggttcgtt
     1501 cccgcggttc gggccgggtt gcaaagcgcg tctttcctgc atttcaggaa gatatccctg
     1561 gaatcttttt ggccgtcgca aaagaatcgg taaaccgttg cataaaaagc gccggtgatt
     1621 gcagtcgaga ttggccgtaa ttctctgaga gagagttagt tagtaccgga cggttggggg
     1681 tggtttggcg tcgatttggg ctatttctgc agccgtaact gtccgataag gataaaagag
     1741 gtataccggc taaacgaacc aacacgagga tcccggtgtc gcttgagaag cacaaagtgc
     1801 tggtggtcga tgatgaactt ttaatccgcg atttgttata cgatttcttc tccggccgcg
     1861 aatgtgagat agcggtcgcc gcttcgggac aggaggcgct gaagcagttg gagcagcatt
     1921 ctttcgatgt cattctggtc gatctgaaaa tgccggacat ggatgggttg gacctggccc
     1981 gggcaatcag agcttccggg cacacgagcc cgatcatcct catcaccggc taccccagcg
     2041 tggaatcggc ggtggaagca ctgcggattc ggctgtacga ctatgtaacg aaaccgttca
     2101 acatcaatca gctgttccac ctggtgagcc gggcggcccg ggagtaccgc gagcaggggg
     2161 aaaaagttta aatgtccgct ccggagactc cgcaccagtc ggcggttccg cagattctcg
     2221 tcattgaaga tgaactctac ctgcgggaga cactggctga ctacctgggc cagcaggggt
     2281 accgggtccg cacctgcgag tccggagagc agggcgtgtt gttgcaccag gaagaacgat
     2341 ccgacgtggc gctcatcgat ctctggctgc cgggacggga cggtctggag atcactcgcc
     2401 tcctcctgga gcaggatgcg tctttacgca tcgtattgat gacctgtcgg cccaatctgg
     2461 tcagtgcaac ggatgcactg cgggaacagg tctttgacta cctgatcaag ccgttccgcc
     2521 tgccggaggt gcgggacgtg gtggagcggg ccgtgcgcca cgccgggctt tgcatccccg
     2581 caccgccgca ggttgaaccg ttcggcccac cggggccgga cgaatatccc gcctccggcg
     2641 gaacgctccg gcaattcccg cctctggttt ggcgcttcgt tttttgattc gtccccaaaa
     2701 tttctcgttg gcagaggggg cgtttacgct ttatatttct ttttcgatgt gcgggataaa
     2761 agtgaagtac agggaaggtg gcgaggcgcc cggcacgtga atcaaaagca gcaaaccgtt
     2821 ccttttaaaa caaccgtcct ggcaccgctg gacacttctt cggctgcgcc ggcggaagat
     2881 gtcactgtac tgcaacaggc atatcgcgaa ctggagcaac ggtgccggtc actcacagcc
     2941 gagctggacc ggctgaaacc tttggcggca ttaggggagc ggatcgccct ggtggcgcac
     3001 gaactccgca atcctttagg cggcatcgcc ggttttgcgg cgatcttaga ccacgacctg
     3061 ccggagaatg atccgcgcaa gcccttgatc gggaaaattc tggcaggtat cgagcagttg
     3121 aaccggatgg cggaaggtct tttgcaccgg gcccgtggac cggagatcag accctcccgc
     3181 ttcgacgccg tgcaattcct cgaggatatc accgttcaat gcgaaaccga attacggtcc
     3241 gccggccgcc gggtgattct gcggcgggat tttcccgaaa aaacgctgcc ggtcgatctc
     3301 gatccggaat acttccggca ggcggtgacc aatctcctgg cgaatagttt ccaggcggtg
     3361 ggcgaggagg gggaagtgag ggtttccgcg aacctggaac cttcggagcc gccgaccccg
     3421 cgaaaaacgc tggtggtcaa agtccaggac aacggaccgg ggatgacccc cgagttactg
     3481 caaaaaattt tcaccccttt tttcacaaca aaaacagatg gttccggcct ggggctggcg
     3541 atcgtccgcc aggcggccca agcccacggc ggggaggttg ccgtgacttc cgcccccgga
     3601 aagggagcga cctttaccct ccgcattccg caggacgtac aacggtgaat ccccgggcgc
     3661 gcatcttggt cgtggatgac gaaccgctca tccgcgagtt cttgaacgaa actctttccc
     3721 gctccggatt tttggtagag caggcggcgg cgggggagga ggcctggcag aaaatccagt
     3781 ccgcctcggg cggatacgac gtggtcttca ccgacgtgaa gatgccccag atgaacgggt
     3841 tggaactttt gcggacggtc aaggcgcaca acccggacgt gtcggtggtc gttttgaccg
     3901 gctacgggag catcgccgat gcggtggcgg cgatcaaatt gggcgccttc gattacctga
     3961 ccaaaccctg cacgccggcg cagatcgagc atgccgtcca aaaagccctg gaattccagc
     4021 gcctggtggc ggagaaccgg cagctgcgcc aggaactggt cagccgctac cagtacgcca
     4081 acatcgtcgg aaaatcggct ttgatgcaaa aagtctttgc gctcgtcgat acggtggcct
     4141 ccagccgcgc cacggtgctc atcaccggag agtcggggac cggcaaggag ctcgtggcgc
     4201 acgccattca ctacaattcc ccgcgccggg aggggccgtt cgtcaagctc aactgcgccg
     4261 cgctgccgga ggggctgatc gagagcgaac ttttcggcca cgagcggggc gccttcaccg
     4321 gggccatccg ctcggcccgg ggccggttcg agcgggccga tggcgggacg cttctgcttg
     4381 atgaaatcag cgagatgccc ttgggcctgc aggcgaaact cttgcgcgtg ctgcaggagc
     4441 gggagttcga gcgggtcggc tccggcgata ccattcaggt cgacgtgcgg atcgtggcca
     4501 ccaccaaccg gaacctttcg gcggtcatcc gcgaggggaa gttccgcgag gacctctatt
     4561 tccgtctcaa tgtcgtcaac attcccatcc cacccctccg cgaccggctg gaggatctcc
     4621 cctatctcgt gaaccacttt attcgcaaat acaacgaaga gaacggcaaa aagatcgaag
     4681 ggatttccga aaaagtgctc gcggttttct cccgctacca ctggccgggc aacgtgcggg
     4741 agttggaaaa ctacctcgag cgggcggtgg tgctctcccc cggccccctg ctccagccgt
     4801 ccgacttccc cccggaagtg acgctgggga aaataaccgc gcccaccgac ggggtgaaaa
     4861 tcgggatgag cctccacgac acggaaaagt tgatgattct gaaaaccctg gagtcgcacg
     4921 ccggcaaccg caccaaagcc gccgaagttc tgggaatttc cacccgcacc ctgcggaata
     4981 aactcgaaga gtatggattg aaagagtagt tcatattggc ggagataaaa aagagggcgt
     5041 cctgtcggga cgccctcttg ctttttgctc gatgctcact tcactaacag catccgtttg
     5101 gtgtccacga attcccccac caccagccgg gcaaaataga tgccgctccc cactggtcgc
     5161 cccagttcgt ccttcccatc ccagtgggcg gtgtaaatgc ccgcgggttg ttgagcattt
     5221 actaattccc gcaccttctg acccagaagg ttgtagatgg ccagactcac ctgcgcctct
     5281 tgtgccagcg agtagcgaat gtcggtttcg ggattgaacg gattcgggtg gttctggaaa
     5341 agcgaccaac tgaaagtttg gctttggagg gcgggcggcg tcaccggttt cggatccgag
     5401 ccggcacaca gctgccttgg ccctgtgacc acctgctgac ccgttacgac gctggaatca
     5461 ttcgtttggt ctctggaatg gccttcgatt ctatacaggg tattcagttc caaacagttg
     5521 agcgtatcgg ccttcctgct ggccgagagc acatgttcct gttccacctc aaacgatggg
     5581 gctcttctgg ctcgaatgac atactctttg atcgaatcga ggggattttg attggtgggg
     5641 cgttgccagg tcaccaccac ctggttggtt cccaccagct cacagggaag ggagagccct
     5701 tgaccagtgt ctgtcaccat tgcttccggc attccgctgc caaacccaaa gcagaatgtc
     5761 gtcacgctgg cctgaaagtt ctgcgggcgg atgggtttgg tggtaacctg tatgctcggc
     5821 tgaccagtgt ccactgccac ccctttgaaa ggactcaccc gccatttgta cgtcttgctc
     5881 cccagccggg tgctgtcggt gaaggtcgtg acattcgagg ctacctgtgc ggttgaatcc
     5941 caggtactgc ccgcgtttgg agaccgatag attttgaacc cgtcctcaca cgaccaaccg
     6001 tcgggccagg tcaagattgt tctgtcaggc acggagttgt cgtttgccgt cactaccgga
     6061 cccgtcttcc gcaaaacggt gtaggcaaag actacatctt gcggctgagc gggaaatgta
     6121 cctttcagtg ttccatcggg gttaaagacc cggtacacgt aggtccttag cgtggcgccg
     6181 gtggagctga aggtctttac gtcacagaac cccatcccat gattcggtga ttcagttgag
     6241 aaccctttgg ttcccacgcc ccggccccaa accgtatcca ccaggcctaa agacgtgtca
     6301 aaggcaaccg tggtggtcac tgtatccagg attaccttga atcgggcacc atcgttggga
     6361 atgcctataa cggtgaagaa tttgaagtcc cgtagagtat cggtggagcc ggaaaccgta
     6421 ccgcctgccg ccgtgtagtg ctgaaccaca aggcagttgg ccttctctaa cgcccggcgg
     6481 gcattgatgc ggccgtagcc aaatgagcta tcccaaccgg ccggccctct gtcatccgaa
     6541 gtcttttgaa tcaggttctg gatgtcgtca ttagacagga agttgtcata cccttttaac
     6601 aatgctgcca gaccggctac gtggggaacg gcaaaggagg tgcccgtttc aaagatatat
     6661 gcgttgtttc caggccatgt cgggcaaaga atgtttttcg gattacctcc gttaaaatct
     6721 atgccacccg gcgctactac atcaatgtgt ctaccggtgt ttgagaattg tgctcgatcg
     6781 tctgcgtcgg ttgaggcacc caccgcaatc accccgtgct caaaaccggc cgggtaccgc
     6841 accggattgg gaacaatggc ctgagcgtca ttcatcattg ccgctacagc cgcgcggtcc
     6901 ttt
//
LOCUS       DBNGZD010000268         2126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1917869, whole genome shotgun sequence.
ACCESSION   DBNGZD010000268 DBNGZD010000000
VERSION     DBNGZD010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1917869"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC16GM_07210"
     CDS             <1..539
                     /locus_tag="AC16GM_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013841.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR2923498.1"
                     /translation="RGTGELPGVSYEEITYEGYGPGGVALLVEVVTDNKNRAVSDIRH
                     LFTRNHGNMSEAGSVAWMFERRGLIQVDSQKIGEDDLLAVALDAGAQDMSREGEFYEI
                     ISPVQQFETVRAALEKSRIPIAQAEITLVPQTTVRLEGAQAEQMLKLIEALEDHEDVQ
                     KVFANFDIAEEVMEKLSA"
     gene            559..1179
                     /gene="ruvC"
                     /locus_tag="AC16GM_07215"
     CDS             559..1179
                     /gene="ruvC"
                     /locus_tag="AC16GM_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR2923499.1"
                     /translation="MGKKFDPTCGVRIIGIDPGLLATGYGVIEVNDLPRENQFRLVCA
                     GALRTSAKISTAERLKQLFDGLCALLSEVRADAAAVEEAFYAQNVRVALTMGHARGAT
                     LVALGSQHLPVYEYATREVKQAVTGQGAAAKGQVQFMVGKLLGCDLSREAHDVTDALA
                     VALCCALRTKAAHSTRHTALRKPLIPKSEILTPMRFDYAHHKGLHG"
     gene            1176..1772
                     /gene="ruvA"
                     /locus_tag="AC16GM_07220"
     CDS             1176..1772
                     /gene="ruvA"
                     /locus_tag="AC16GM_07220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR2923500.1"
                     /translation="MIAYLEGRLVEKQPTAVVLDVNGIGYRVQIPLSSFSALAGAGAN
                     GRVRLFTSFHVREDSHQLFGFATQAEREMFELLLSVSGVGPKAALALLSGLALPTLRR
                     AIAEQNTLTLESVSGVGKKLAARLTLELKDKIGKLSAITGDGALAQKPTDSQEEAVLA
                     LVSLGYTRPEANRKVEQVAKRKDLPVEEVVRQALQTAG"
     gene            1772..>2126
                     /locus_tag="AC16GM_07225"
     CDS             1772..>2126
                     /locus_tag="AC16GM_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2923501.1"
                     /translation="MAQPFKGSGSFRPNTERGAGRKLVLPREGQTKERVTAHVRMQGV
                     LEYDLSLRPTRFEEFVGQKKTIENLKIFIAATKKRGEALDHVLVYGAPGLGKTTLAYI
                     IAKELGVPIKVTSGPV"
BASE COUNT          458 a          573 c          704 g          391 t
ORIGIN      
        1 agcgggggac cggtgaactg ccgggcgtct cctacgagga aattacctac gaaggatacg
       61 gccccggggg tgtggcgctc ttggtggagg tcgtcaccga caacaagaac cgggcagtct
      121 ccgatatccg gcacctcttt acccgcaacc acgggaatat gagcgaagcc ggttcggtgg
      181 cctggatgtt cgagcggcgc gggttgattc aggtcgattc ccagaaaatc ggcgaagacg
      241 accttttagc cgttgcactg gacgccggag cgcaggatat gtcgcgagag ggcgagttct
      301 atgaaatcat ttccccggtg cagcagttcg agacggtaag agcagccctg gagaagagcc
      361 gcatacccat tgcgcaggcg gaaatcaccc tggtgccgca gacgaccgtc cggctcgaag
      421 gcgcgcaggc ggagcagatg ctcaagttga tcgaggcctt agaagaccac gaggacgtgc
      481 agaaggtctt tgccaacttt gacatcgctg aagaggtgat ggaaaagctg agcgcatagg
      541 agctcggctt gaggcgggat gggaaaaaag ttcgatccga cttgcggcgt ccggatcatc
      601 gggatcgatc cggggctttt ggccaccggc tacggtgtga tcgaggttaa cgaccttccc
      661 cgagagaatc agtttcgtct ggtttgcgcc ggcgccctgc gcaccagcgc caagatttca
      721 acggcggagc ggctgaagca attgttcgac ggcctctgtg cgcttttgag tgaggtgcgg
      781 gccgacgcgg cggcggtgga agaagcgttt tacgcgcaga acgtccgggt ggcgctgacc
      841 atggggcacg cccggggagc gaccctggtg gctctgggga gccagcacct gccggtctac
      901 gagtacgcca cccgcgaggt gaagcaggcg gtgaccgggc agggtgcggc ggccaagggg
      961 caggtgcagt tcatggtggg gaaattgctg ggctgcgatt tgtcgcggga agcgcatgac
     1021 gtcaccgatg ctttggcggt agcgctttgc tgtgcgctcc gaacgaaggc agcacacagt
     1081 acacggcaca cagcactcag gaaaccccta attcctaaat ccgaaatcct aactccgatg
     1141 cggttcgact acgctcacca caaggggttg cacggatgat cgcctacctc gaaggccgtc
     1201 tggtggaaaa gcaacccaca gcggtggtcc tcgacgtcaa cgggatcggc taccgagtgc
     1261 agattccgct ttccagtttt tccgccttag ccggagcggg ggccaacggc cgggtgaggc
     1321 tctttacgtc attccacgtg cgcgaggatt cccaccagtt gttcggcttt gccacgcagg
     1381 cagagcggga gatgtttgaa ttgctgctct ccgtctccgg cgtcggtccg aaagcggcgc
     1441 tggcgctttt gtcgggtctc gctttgccca cgctgcggcg ggcgattgcc gagcaaaata
     1501 cgttgacctt agaaagcgtt tcgggcgtgg gaaaaaaatt agcggcgcga ttgacattgg
     1561 agttgaagga caaaatcggc aaactgtcag ccatcaccgg tgacggtgcc ctggcgcaaa
     1621 agccgaccga cagtcaggaa gaggcggttt tagctctggt ttccctcggc tatacgcggc
     1681 ccgaggccaa tcggaaagtg gagcaggtag ctaaacgcaa agacctgccg gtagaggaag
     1741 tagtgcgtca ggccttgcaa acggcgggat aatggcgcaa ccgttcaaag gttctggaag
     1801 ctttagaccc aataccgagc gaggcgccgg gcggaaactg gttttgccgc gcgaaggcca
     1861 gaccaaagaa cgggtcaccg cgcacgtgcg gatgcagggg gtgttagaat acgacctgtc
     1921 gctccggccc actaggtttg aggagttcgt cgggcagaag aaaaccatcg aaaacttgaa
     1981 aatcttcatc gccgcgacga aaaagcgcgg cgaggccttg gatcacgtgc tcgtctacgg
     2041 cgcgccgggc ttgggcaaga cgaccctggc ttacatcatc gccaaagaat taggagtacc
     2101 aatcaaggtg acctccgggc cggtgt
//
LOCUS       DBNGZD010000269         6563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_667005, whole genome shotgun sequence.
ACCESSION   DBNGZD010000269 DBNGZD010000000
VERSION     DBNGZD010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_667005"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC16GM_07230"
     CDS             <1..348
                     /locus_tag="AC16GM_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR2923502.1"
                     /translation="TGRLIRASLRVGALLADARSEKLAVVSCFGEKLGLAFQIADDIL
                     DAEGQTGVLGKTGYSDRRKKKATHPGVVGLAASKKRVQELIDEAKTIGRSAFPDSDAL
                     FLLADFVLHRAGQ"
     gene            362..2299
                     /gene="dxs"
                     /locus_tag="AC16GM_07235"
     CDS             362..2299
                     /gene="dxs"
                     /locus_tag="AC16GM_07235"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR2923503.1"
                     /translation="MSPFLSQIHSPADLKTLSQKELVSLAAEIREKILEVVSANGGHL
                     ATNLGSVELTLALHRVFDSPQDQIIWDVGNQAYAHKLVTGRYEKFATIRKQGGLSGFL
                     RRDESPHDQFGAGHASTGISAGTGFACGRDLAGGKQQVVVIVGDGALTGGLSFEGLNN
                     AGALGKNLLVVLNDNSMSISKNVGAIAKFLTTFLTDEHYNKLKTDIWELTGRSKQGRK
                     IREIISHIDENIKGFFVPGLLFERLGFRYFGPIDGHDIPQLIRVFTHLKTLPGPLLLH
                     VLTVKGKGYVPAEHDPAKFHGVGAFDKVTGKANAAGETAYQAVFGETLVKIAEKNRKV
                     VAITAAMCTGTGLVEFAKKFPERFFDVGIAEEHGATFAAGLACQGIKPVYAIYSTFLQ
                     RAFDQVVHDVALQNIPVLFAVDRAGLVGDDGPTHHGVFDLSYLRAIPNFTVAAPKDGN
                     EFKDLLWSAFDWQDGPVAIRYPRGGIPDKLQDAVRPIPYGTWQVEREGEEVVIFAVGA
                     MVYPALEAATRLLVDGLSPTVVNARFIKPLDEKLLALLLENHKKVITVEDNVLAGGFG
                     SAILEWIEAAGKTGFTVKRLGIPDQFITHGPRKLLLNQLGLDADGLVRTVRALVPAKV
                     PKQTLRLARTLPEAVPPAPQP"
     gene            2302..3180
                     /locus_tag="AC16GM_07240"
     CDS             2302..3180
                     /locus_tag="AC16GM_07240"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR2923504.1"
                     /translation="MRLGINANVERPDCETALRRVLRWAAEHKISFSIAEHLVPLVGH
                     PEAACPLPLLPEKSDHILSLGGDGTFLATARAVAARGTPVLGLNVGGFGFLTTISVDN
                     LEKNLDRVRSRAYLLEERMVLEGAIEDAGAPAFALNDVVVEKGTTARLIHLALYVNGE
                     YVSSYSADGLIVATPTGSTAYSLSVGGPIINPKINAIIVAPVSPHALTSRPIVFSEKD
                     EIRIVLAGVRQKAAVSLDGQVNFELLPGQAVAVKKAPHTLKVVTFPETSFYDVLRRKF
                     HWGGIPAAGKQAVTEP"
     gene            3187..3993
                     /gene="mtgA"
                     /locus_tag="AC16GM_07245"
     CDS             3187..3993
                     /gene="mtgA"
                     /locus_tag="AC16GM_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR2923505.1"
                     /translation="MRFFATRRGKIWGLSGGLAGLVLLWALAEYLSVPDVSALARQNP
                     ETTALIERRQAEYRARKEKVERCQFWVPFSQISPYLKRAVLVAEDISFYEHGGYDLAE
                     IKRAIKINLKEKRFARGASTLTQQLAKNLYLSTSKNPLRKLRELFIALALEKHLSKRR
                     IFELYLNNIEWGRGIYGVEAAALNYFNKSATDLSPDEAARLAAVIPDPLRLTPFDSSR
                     RFEQKRKLILTRMEQYGFLTHEEYAQALAPVARESDTRAGRVGDSALEPQ"
     gene            4033..4791
                     /locus_tag="AC16GM_07250"
     CDS             4033..4791
                     /locus_tag="AC16GM_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR2923506.1"
                     /translation="MRLVKITLWQKEADGDLSAATLSEISSTRPDFLCLPEYCNASRE
                     TRHQFDIASRFPQYRLTMVEISRRLQTTVIGGSAVEQVGDRYYNTCIVASRGKELGGY
                     HKIHLFAREQGKGLATGNEYKVFEAGGVRFGVLICADVLYEESFLEMRQLRPKLIFIP
                     TTSPFKPNDTLEQKLARDKEIFQRGAELSQAYVVKVCAVGTLFGGRLQGRSLVAAPWG
                     ILWRVSPEDEEKELIQAYELDLDQLRHWQTERQP"
     gene            4799..>6563
                     /locus_tag="AC16GM_07255"
     CDS             4799..>6563
                     /locus_tag="AC16GM_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923507.1"
                     /translation="MRRTILIVLWTCSFLMMGGRRCPPYGFAGTALAQEDPFDQALAK
                     VGLTRETLRFDDDDMANFGGDKFLLPLFTTLHRNPLKIPDYTRVFRDAVIQNAGSLEN
                     MLTFASLRLKAGVRRNLVANPIAGAVEKSKEPEALYQSILALYAKDGRSLRGEEKAQI
                     QKRCSQVPVELANLIAVFVYTTVSSKGYLDEAFAEAHDLPMNKIRNQALRLLVADSDL
                     TTPQFERFAEKVDFPYLYAGAEDLAWAVDLISDSLSRLTVLLQHNLTISTPWGVIAVN
                     GTGNDLVTEDHPYLLIIDLGGDDTYQTGGATTRSEQPASVLIDLAGNDRYVNSDSLRP
                     AFGAGVFGYGFLVDLGGNDQYESVNNSLGVGLFGFGTLLDKSGNDQYRARTTSQGAGI
                     FGIGLLSDLDGDDRYYACLAAQGFGYTKGCGILVDLRGSDEYVADDSTVVFPSAQTKE
                     HNDSMCQGVGFGRRADITDGHSWAGGLGFLVDAAGDDRYSAGLFAQGCAYWYGVGILA
                     DQAGNDTYHGVWYVQGSGAHFGVGILDDVAGNDHYTATMNMAQGAGHDFTVGFLIEEA
                     GDDTYQAPNLSLGGGNDNGIGI"
BASE COUNT         1312 a         1970 c         1928 g         1353 t
ORIGIN      
        1 accggcagat taattcgggc cagcctgcgc gtgggagcgc ttctggctga cgcccgttcc
       61 gaaaagctcg cggtggtcag ctgttttggg gagaaattgg ggttggcttt tcaaatcgca
      121 gacgatattt tagatgccga aggccagacc ggtgttctgg gaaaaaccgg ctattcggac
      181 cgtcgcaaaa agaaagcgac ccaccccgga gtggttggat tggcagcgtc aaaaaaacgg
      241 gtccaagagt tgatcgacga ggcgaaaacc attggccgtt ccgcctttcc ggacagcgac
      301 gcgcttttcc ttttggctga tttcgtcctc caccgtgccg gacagtaact tcgctatccc
      361 gatgagtcca tttttatcac agatacattc gccggccgat ctcaagacac tctcccaaaa
      421 ggaactggta tccctggcgg ccgagatccg cgaaaaaatt ttggaagtgg tttcagccaa
      481 cggcgggcat ttggctacca acttaggttc ggtggaactg accctggcat tgcaccgggt
      541 cttcgattcg ccccaagacc agatcatctg ggacgtgggg aaccaggcct acgcgcacaa
      601 actggtcacc ggccgctacg aaaagttcgc gaccatccgg aagcaggggg gactctccgg
      661 tttcctgcgc cgggatgaga gcccgcacga ccagttcggc gcggggcacg cctccaccgg
      721 catctcggcc ggcacgggct ttgcctgcgg acgtgatctg gcgggcggca aacaacaggt
      781 ggtggtcatc gtgggggacg gcgcgcttac cggcggtctc tccttcgaag ggctcaacaa
      841 tgccggcgct ctgggcaaga acctcttagt tgtgctcaac gacaactcga tgtcgatttc
      901 gaaaaacgtg ggggcgattg ccaagttcct cactaccttt ctcaccgacg agcactacaa
      961 caaattgaaa accgacatct gggaactgac cggccgctcc aaacagggtc ggaagattcg
     1021 ggagatcatt tcccacatcg acgagaacat caagggcttc ttcgtcccgg gcctcctctt
     1081 cgagcggctg gggttccgct acttcgggcc catcgacggc cacgacattc ctcagctcat
     1141 ccgcgtgttc acccacctca aaacgctgcc cggaccgctc ctgttgcacg ttttgaccgt
     1201 gaaaggcaag ggctatgttc cggcagaaca tgatccggcg aagttccacg gcgtgggggc
     1261 cttcgataag gtgaccggca aagcgaatgc cgcgggcgaa acagcctacc aggcggtctt
     1321 cggcgagacg ctggtcaaaa tcgccgagaa aaatcggaag gtggtggcga tcaccgccgc
     1381 tatgtgcacc ggtacggggt tggtcgaatt cgccaaaaag tttccggagc gtttctttga
     1441 cgtgggcatt gccgaagagc acggggcgac gtttgccgcc ggcctggcct gccagggaat
     1501 aaaaccggtc tatgccatct actcgacctt tctgcagcgg gcgtttgacc aggtcgtcca
     1561 tgatgtggcc ctgcagaata tcccggtctt gtttgcggtc gatcgcgccg gtttagtcgg
     1621 cgacgacggc cccacgcacc acggggtctt cgatctctct tacctgcggg cgatccccaa
     1681 tttcaccgtg gccgcgccga aggacggcaa tgaattcaaa gacctgctct ggtcggcttt
     1741 tgactggcaa gacggcccgg tggccatccg ctacccgcgc ggcgggatac ccgataaact
     1801 tcaggatgcg gtccgcccga ttccctacgg cacctggcag gtagagcgcg agggggaaga
     1861 ggtggtaatt ttcgccgtcg gcgcaatggt gtatccggcg ctggaagccg ccacgcggct
     1921 attagtagat ggtctttctc cgaccgtggt gaatgcccgc ttcatcaagc ccctggacga
     1981 gaaactcctg gcacttctcc tggagaacca caagaaagtc atcaccgtgg aggacaatgt
     2041 actggcgggc ggattcggtt cggccattct cgaatggatc gaagccgcag gcaaaaccgg
     2101 ctttaccgtc aaacgtctgg ggattcccga ccagttcatc acccacggcc cgcgcaagct
     2161 gctcttaaac cagcttggcc tggatgccga cggtctcgtc cggaccgtca gagcgctcgt
     2221 gcctgccaaa gttccgaagc agacgttgcg cctggcgcgg acgctgcccg aagcggtccc
     2281 ccctgctcca caaccctaac gatgcgtctg gggattaatg ccaacgttga gcgcccggac
     2341 tgcgagaccg ccctgcggcg cgttttgcgc tgggccgccg aacataaaat ctccttctcc
     2401 atagctgagc acctggtccc cctggtgggg cacccggagg cggcctgtcc gctccccctg
     2461 ttacccgaaa agagcgatca tatcctctct ctgggcggag atgggacttt tctggccacg
     2521 gcccgggcgg tggctgcccg gggcacgccg gttttgggcc tgaacgtcgg cgggttcggc
     2581 tttctcacga cgatttcagt ggacaatttg gaaaaaaatc tcgaccgggt gcgctcccga
     2641 gcttacctct tagaagagcg gatggtgctg gagggggcga ttgaggacgc gggcgcacct
     2701 gcctttgcgc tcaacgacgt ggtggtggaa aagggcacta ccgcccgctt gattcacctc
     2761 gctttgtacg tgaatggtga gtacgtttca tcctactcgg cggacgggtt gatcgtcgcc
     2821 acaccgaccg gctcgacggc ctacagcctc tcggtcggcg ggccgatcat taatccgaaa
     2881 atcaacgcca tcatcgtggc gccggtgagc ccgcacgcgc tcacctcgcg gccgatcgtt
     2941 ttttcggaaa aagacgaaat ccggatcgtt ctggccggtg tccggcagaa agcggcggtg
     3001 agtctggacg gccaggtgaa ttttgaactc ttgcccggcc aggcggtggc ggtcaagaag
     3061 gccccgcaca ccctcaaggt cgttaccttt ccggaaacct cgttctacga cgtgctccgg
     3121 cgcaagttcc actggggcgg aattcccgcc gccgggaagc aggccgttac cgaaccgtaa
     3181 gcggccatgc gctttttcgc cacgcgccgc ggcaaaatct ggggcctgtc gggcggcttg
     3241 gccggactcg ttctcctgtg ggctctggcc gagtatctct ccgtcccgga cgtttcggcg
     3301 ctcgcccggc agaatccgga gaccaccgct ctgatcgaac ggcgccaggc ggagtatcgt
     3361 gcgcggaaag aaaaggtgga gcgctgccag ttctgggtcc ccttcagcca gatttctccc
     3421 tacctcaagc gggcggtcct ggtggccgaa gatatttcct tctacgaaca cggcgggtac
     3481 gacttagccg agatcaagcg ggcgatcaag attaacctga aggaaaagcg cttcgcgcgc
     3541 ggcgcctcta ccctcaccca gcagttggcc aaaaacttgt atctctccac ttcaaaaaat
     3601 cccctccgca aattgcgcga attgttcatc gccctggccc tggagaaaca cctttccaag
     3661 cgccgcattt ttgaactgta tctgaacaac atcgaatggg ggagaggcat ttacggcgtc
     3721 gaggccgccg cgctcaacta cttcaacaaa tcggcgaccg acctctcgcc cgacgaagca
     3781 gcccgcctgg ccgccgtaat ccccgatccg ctccgcctga caccgttcga ctcttcccgg
     3841 cgcttcgagc agaagcgaaa attaattttg acgcgcatgg agcagtacgg ttttcttact
     3901 cacgaggagt atgcccaagc gcttgctccg gtcgcgaggg agtcagatac aagagcggga
     3961 agggtcggcg attccgctct tgaaccccaa taaactctag acaccctgtt tgcaggcggg
     4021 cagtaggtca gcgtgcgctt agtgaaaatt acgctctggc agaaggaggc ggatggagat
     4081 ttgtctgcgg ccactctctc tgaaatttcg agtacccgac cggatttctt atgcctcccg
     4141 gaatactgca atgcttcccg ggaaacccgc caccagtttg acattgcttc ccggtttcct
     4201 caatatcgtt tgacgatggt agaaatctcc agacggctcc agaccaccgt catcggcggc
     4261 agtgcggtag agcaggtcgg cgatcgctac tacaatactt gcatcgttgc cagccgcgga
     4321 aaggaattgg gtggctacca caaaattcac ctctttgccc gggagcaggg gaaaggactg
     4381 gcgaccggca acgagtacaa ggtcttcgaa gccggaggcg tccgtttcgg ggtattgatc
     4441 tgtgccgatg tgctctacga agaatcgttt ctggagatgc gtcagctacg gccgaagctt
     4501 atcttcatcc cgaccacttc gcccttcaag ccgaatgaca cgctggagca gaaactggcc
     4561 cgcgacaaag aaattttcca gcggggggcc gaattgagcc aggcgtacgt ggtcaaagtc
     4621 tgtgccgtcg gcacgctctt cggcgggcgg cttcaggggc gcagtctggt tgccgcgccc
     4681 tggggcatcc tctggcgcgt gtcaccggaa gacgaggaga aagagctcat tcaagcctac
     4741 gaactcgatt tggaccaact gcgtcactgg caaaccgaac gccaaccctg aggaatctat
     4801 gcgacgaacc atcctgatcg ttctctggac gtgctcgttt ttaatgatgg gtgggcgaag
     4861 atgcccgccc tacgggtttg ccggtaccgc gctcgcgcag gaggatcctt tcgaccaagc
     4921 gctggcgaaa gtcggcctca cgcgtgaaac gctgcgcttt gacgacgatg acatggccaa
     4981 tttcggcggc gataagttcc tcttgccgct cttcaccacg ctccaccgca acccgctcaa
     5041 gattcccgat tacacgcgcg tttttcggga cgcggttatc caaaacgccg gttctctgga
     5101 aaatatgctc accttcgcat ccctgcggct caaagccgga gtgcggcgaa atctggtcgc
     5161 caatccgatt gcgggagcgg tcgaaaaatc caaagagccg gaggcgctct accagtcgat
     5221 tctggctctc tacgccaaag acggccgctc cctgcggggg gaagagaaag cccagattca
     5281 gaagcgctgt tcccaggtgc cggtggaact ggccaatttg atcgcagtat tcgtctacac
     5341 taccgtcagc tccaaaggtt atctcgacga agcctttgcc gaagcccacg atcttcccat
     5401 gaacaaaatc cgcaatcagg cgttgcggct tttggtggcc gacagcgacc tgaccacgcc
     5461 gcagttcgag cgttttgccg aaaaggtcga tttcccctac ctctacgccg gggcggaaga
     5521 cctggcctgg gcggtcgatt tgatttcgga ttcactctcc agactgaccg tgttgctcca
     5581 gcataacctg acgatttcca ccccctgggg tgtgattgcc gtcaatggta ccggaaacga
     5641 tctggttacg gaagatcatc cttacttgct catcattgac ttgggtggtg acgacacgta
     5701 tcagaccggc ggcgccacga cccggtctga gcagcccgcc tccgtgctca tcgacctcgc
     5761 cggcaatgac cggtacgtga actccgattc gctccgccca gccttcggtg ccggagtttt
     5821 cggctacggt tttctggtcg atctgggcgg caacgaccag tacgagagcg tcaataattc
     5881 cctgggggtc ggcctctttg gcttcgggac gcttctggat aaaagcggca atgaccagta
     5941 tcgcgcccgg actacttctc agggggcggg aattttcggc atcggtctgc tctccgatct
     6001 ggatggcgac gaccgctact atgcctgcct ggcggcgcag ggcttcggtt ataccaaagg
     6061 gtgtggcata ctcgttgatc ttcgcggcag tgatgaatat gtcgccgatg acagcaccgt
     6121 cgttttccct tcggcgcaga ctaaggagca taacgacagt atgtgccagg gcgtgggatt
     6181 tggccgacgt gcggacatta ccgatggcca ctcctgggcc ggcggtctcg gatttttggt
     6241 ggatgcggcc ggcgacgacc gttattccgc cggcctcttt gctcagggct gtgcctactg
     6301 gtacggcgtg ggtatcctcg ccgatcaggc cgggaacgat acctatcacg gtgtctggta
     6361 cgttcagggc tccggggctc atttcggcgt cggcattctt gacgacgtag ccggcaatga
     6421 ccattacacc gctaccatga acatggccca gggcgccggg cacgacttca cggtcggttt
     6481 cctcattgag gaagccggtg acgacaccta ccaggcgccc aacctgagtt tgggcggcgg
     6541 caacgacaac ggcatcggca tct
//
LOCUS       DBNGZD010000270         2759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1776618, whole genome shotgun sequence.
ACCESSION   DBNGZD010000270 DBNGZD010000000
VERSION     DBNGZD010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1776618"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="AC16GM_07260"
     CDS             <1..852
                     /locus_tag="AC16GM_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR2923508.1"
                     /translation="EMHVTSYCQITCWGRGASLKNPAAFWYDEFKERILVANTGANAV
                     DVFTAEGQPLFRIGEQGELSLPVGIAASPAGKIFILERNSPLLKIYDEETKKTDTFNL
                     AGVDSTRKVSISRISADREGNLLLVDAENDRILVLDRSLRLAAKVGSSGRGEGKLQEI
                     VALAADNSGKIFVADALNYPVQVFDRKGRFLFRFEKSAIAERSVWKPAGMFLDPRNRL
                     WVIDAVAASFRVYDPNGNLLQKVEAADGGTKKFFSPIQIAADKYGFLYVLEKGKDRID
                     LLRIENF"
     gene            856..>2759
                     /locus_tag="AC16GM_07265"
     CDS             856..>2759
                     /locus_tag="AC16GM_07265"
                     /inference="COORDINATES: protein motif:HMM:NF021235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2923509.1"
                     /translation="MKRSAWAIGSLLGFVAALLVRAQTGNYDTSPHGDPNTGVLRDNR
                     FARGECSQCHLAHKDITPEALLLFTENNNNLCYTCHSQKPIGYPAQESDRLPIASTYP
                     GYFEYNSNGVKITGVENRKRWPGQLVYENSGSSASGKYFSPHRSDLDMPRKDNNGSGM
                     CLNCHNPHGTRNPFDMLDTTYLNIQGAFVSGRPGNYALCFKCHSTDGPAGMNPENKRI
                     ADYYDKSVNNDGESGHQFKTTFGYVRAGDKLPCYDCHNPHGSQGNDGLHPNTFLLSDQ
                     RAGWSGLDSLQTSSTQVRRFCFGCHKSSDGQGGGTVEGVTLAALPSSVAEHAFNDQTH
                     CYECHGRDYSSPTTRNVHHPLPGGDCLSCHSMAQGDPASPTRRRAITGQAGDFVKVAH
                     HVTNGTGIEVVTKFDCGVCHMEGDPLTGRTNSTYHKNGVLDLRNPDTGGAIAGFTRFS
                     RDTLSNNLESWVTSVQNNLCLKCHDADGAASPLARTQLGTPQRPFTSQTKDALDVSGL
                     LQSVNNFHHAVLFPGNNPYCTPTATNGNVVTMNPPWNQIANTHNRISCFDCHAVNGHG
                     GNFSGILRRETYYKDPVVNPNFANAEKNFCTICHKLTSYYLGSGGSRLNEHDKSEHRT
                     VADGGRNQYG"
BASE COUNT          626 a          831 c          758 g          544 t
ORIGIN      
        1 gaaatgcacg tcacctcgta ctgccagatt acttgctggg ggagaggagc atctctgaaa
       61 aatccggccg cgttctggta cgatgagttc aaagagcgga ttttggtcgc caacaccggc
      121 gccaatgcag tggacgtttt cacggcggag ggccagcccc tctttcgcat cggtgaacaa
      181 ggggaactct ccctcccggt cggcattgcc gcaagccctg ccggcaaaat ctttattctg
      241 gagcggaaca gtccgctctt gaaaatttat gacgaggaga caaaaaagac tgacaccttc
      301 aatctggccg gggtagattc cacccggaag gtttccatca gccggatttc cgccgatcgg
      361 gaggggaacc tccttctggt cgatgcggaa aatgaccgaa ttctggttct ggataggtcc
      421 ttgcggctcg ccgccaaggt gggctcctcc gggcgcgggg aggggaaact ccaggagatc
      481 gtggccttgg cggcggacaa ttccggcaaa atctttgtgg ccgacgctct gaattatccg
      541 gtacaggtct ttgaccggaa aggaaggttt ttgttccgct ttgaaaaatc ggccatcgcg
      601 gaacggagcg tctggaaacc ggccgggatg ttcttagacc cccggaaccg cctctgggtc
      661 atcgatgcag tggcggcgag ttttcgcgtg tatgatccca acggcaacct cttgcagaag
      721 gttgaggctg ccgacggagg aacgaaaaaa tttttctcgc cgatacaaat agcggcggat
      781 aaatacgggt tcctgtatgt cctggaaaaa ggaaaagacc ggattgacct gctcaggatt
      841 gaaaactttt agcttatgaa acgttccgct tgggccatcg gttcgctcct cggcttcgtg
      901 gccgcgcttt tggttcgggc gcagacggga aattacgaca cctcgccgca cggcgatccg
      961 aacacgggcg ttctgcggga taaccgcttt gcccggggag agtgcagcca gtgccacctg
     1021 gcgcacaagg atataacacc ggaagcactt ttgctcttca ccgaaaacaa caacaacctc
     1081 tgctacacct gccactccca gaagccgatc ggctatccgg cgcaggaaag cgaccgcctg
     1141 ccgatcgcca gcacctatcc cggctacttt gaatacaact ccaacggggt caaaatcacc
     1201 ggagtggaaa accgcaaacg gtggcccgga caactggtct acgagaacag cggcagttcg
     1261 gcctcaggaa aatatttctc cccccaccgt tcggatctcg acatgccccg caaggataat
     1321 aacggctcgg gcatgtgcct gaactgccat aatccccacg gcacccgcaa tccctttgac
     1381 atgctggaca caacttacct gaacatccag ggggctttcg tctccgggcg tccggggaat
     1441 tacgcgctct gtttcaaatg ccattccacg gacggcccgg ccggtatgaa tccggaaaat
     1501 aaacggattg cggattacta cgacaaatcg gtcaataacg acggcgagtc cgggcaccag
     1561 ttcaagacca ccttcggcta cgtccgggcc ggcgacaaac tgccctgcta cgactgtcac
     1621 aatccccacg ggtcgcaggg gaacgacggc ctgcacccga ataccttcct cttgagtgac
     1681 caaagggccg gctggtccgg cctggattcc ctgcaaactt caagcaccca ggtcagacgc
     1741 ttctgtttcg gctgccacaa atcctcggac gggcaggggg gcggaacggt ggaaggagtg
     1801 accttagccg ccttgcccag tagtgtcgcc gagcacgcct ttaacgacca gacgcattgt
     1861 tacgagtgcc acggccggga ttactcttcg cccacgacgc ggaacgtcca ccacccctta
     1921 cccgggggag attgtctctc ctgccattcc atggctcagg gcgatcccgc ttcgccgacc
     1981 cgccgcagag cgatcaccgg ccaagccggt gatttcgtga aagtcgcgca ccatgtgacg
     2041 aacggaaccg gaatagaggt cgtcaccaag tttgactgcg gcgtctgcca tatggaaggg
     2101 gatcctttga ccggccggac caactccacc taccataaaa atggggttct agacctgcgg
     2161 aatcccgaca ccggcggcgc cattgccggt tttacccgct tcagccggga caccctttcg
     2221 aataatcttg aatcctgggt gacgagtgtg cagaacaatc tttgcctgaa atgccacgat
     2281 gccgacggtg cggcttcacc tctggcacgt acccagctgg gtacgccgca gcggcccttt
     2341 acttcccaga cgaaagatgc cctggatgtg agtggtctcc tgcagagcgt caacaatttc
     2401 catcacgccg tcttattccc cgggaataat ccgtactgca cgccgaccgc taccaacggc
     2461 aatgtcgtca ccatgaaccc gccctggaat cagatcgcca acacccacaa ccggatttcc
     2521 tgttttgact gccatgcggt caacggccat gggggaaact tctccgggat actgcggcgc
     2581 gaaacctact acaaagaccc cgtggtcaat ccgaactttg ccaacgccga gaaaaacttc
     2641 tgcaccatct gtcataagct gacgtcgtac tatctcggtt ccggcggcag ccgtctgaac
     2701 gagcacgata agagtgagca ccgaaccgtg gctgacggcg gccgaaatca gtacggctg
//
LOCUS       DBNGZD010000271         5700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1080846, whole genome shotgun sequence.
ACCESSION   DBNGZD010000271 DBNGZD010000000
VERSION     DBNGZD010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1080846"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="AC16GM_07270"
     CDS             <1..680
                     /locus_tag="AC16GM_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923510.1"
                     /translation="AVAFPISLTRSKSLVLGNSLTGPGINGLDIGANMNKLQYNPATV
                     SQDTVVGRFTNALSFQGGNDFGAQANVTNCVLTNDIGSASTGILYNGDTVKVSFTKDT
                     AFGAYGAVMNSPGFVLTTDNCIFSDTAIATKFVDNNATSVTLDARSNYWFFTDTSTIR
                     GELDGPINIRPINTTCESGPGQKPVTPEASTQKPLVFALYANAPNPFNPARDCSSPCR
                     SRGKLNW"
     gene            677..862
                     /locus_tag="AC16GM_07275"
     CDS             677..862
                     /locus_tag="AC16GM_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2923511.1"
                     /translation="MIFNLLGQKVRTVVRGAWLAGVHEVIWDGKDDRNQTLASGIYFA
                     RLRAGENQQVQRMVMLK"
     gene            859..1362
                     /locus_tag="AC16GM_07280"
     CDS             859..1362
                     /locus_tag="AC16GM_07280"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HIR2923512.1"
                     /translation="MTGNKVLLTGVPLLLFALAGYALAGQVTVPLTGSAQAVENGTPQ
                     ILLKFDLPPELARAHIDHATLRVGLVSEAKAVPVRLLLITDDWSPSATDLVDPHIAVT
                     DSLSTGKVLSAGAVSLDVTDLVQALADGAMPNRGLMLVPVKPLTTFRLGVARVQVSIF
                     YTPEEQH"
     gene            complement(1470..2495)
                     /locus_tag="AC16GM_07285"
     CDS             complement(1470..2495)
                     /locus_tag="AC16GM_07285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR2923513.1"
                     /translation="MTKRLSSALVMAVLTHLLLAAVVWAQAPPAPSALPDYLRSAGMV
                     EKPVEVVTDSVRLPALLTLPDSTGKGFPIVVLVHGSGPNDKDETVGPNKPFRDLAYGL
                     AKQGIATLRYDKRTRAGYTQVKLLEDEVILDALSALRLAKSFPQVDSAKVFLLGHSLG
                     ATVAPYIARRAPGLAGVIALAGLTQPWDATLLRQMKTMMQTQGVDSVSIAVQQKQLRE
                     GFERIKSGSLPDDSMFFYAPASYWRDLFALHQAEAAAQSKLPFLFLQGGYDHQVLADQ
                     DYTGWQQAFAAAKKPFTGKVYPTLNHLFIAQDKAGSPLDIMKPGWVAPEVITDLAAWI
                     KTGKLKP"
     gene            complement(2575..3315)
                     /gene="tatC"
                     /locus_tag="AC16GM_07290"
     CDS             complement(2575..3315)
                     /gene="tatC"
                     /locus_tag="AC16GM_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR2923514.1"
                     /translation="MPRDSKAMPFLAHLEELRWRIIKCLASILVGSVVIYFFSDQMLD
                     FLTRPVRAVYFSGVTEAFAVRIKLSLYGGLILSVPVILYQAWQFVVPGLLEREVKVAL
                     PVVVAATLFFAWGGAFCFFVVLPAGIKFLLTFGSAKLQPLITVDKYVSFVSFMVIAFG
                     AVFELPVLAFVLGQLGIISHRTLAKGRRYAVVLIVILAAILTPTPDAFSQLMLAVPLY
                     FLYELSILVVRLTGRRKLGNTVRVLTPN"
     gene            complement(3444..3896)
                     /gene="nrdR"
                     /locus_tag="AC16GM_07295"
     CDS             complement(3444..3896)
                     /gene="nrdR"
                     /locus_tag="AC16GM_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR2923515.1"
                     /translation="MKCPYCGHEEDKVVDSRTAQDGRAVRRRRECLACGERYTTYEYV
                     ENRELTLVKNDGRREPFDRQKLRRGIELACNKRPVSAKKIDAVVDEIEEELQNLSKGE
                     ISSQEVGEKVMEKLKGLDDIAYVRFASVYRKFQDIEHFQEELNKLLNK"
     gene            complement(3992..4831)
                     /locus_tag="AC16GM_07300"
     CDS             complement(3992..4831)
                     /locus_tag="AC16GM_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR2923516.1"
                     /translation="MTARHESHSTGPLVTFTSDFGSADAYVPAVKGVIASINPRARII
                     DTIHQIPPHNIVRGALMLKGFVPYYPPGTIHLAVVDPGVGSKRRALLIVTDHGFFVGP
                     DNGLFALVVPASGHKKVIHLKEKRFWLNPQSPVFHARDIFAPVAAYLSRGVKPEEFGP
                     EVKGIKSLAIPKPHRSAAAIRGDVISVDRFGNLTTNIPGEWLLQKVAGSREPVARRQF
                     KIHSGPHELAGLFETYALIPPKKAGAVIGSAGFLEVALNRASAAERLQANVGTLVAIR
                     LSG"
     gene            complement(4828..5250)
                     /locus_tag="AC16GM_07305"
     CDS             complement(4828..5250)
                     /locus_tag="AC16GM_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIR2923517.1"
                     /translation="MQFQRIPSGYLVRLVKGEEIITTLAGFVEREKVKGGGFTGLGAM
                     ADVELGYFDAHTREYVRKEYPGEYEIGNLTGNIAWLDGKPVVHAHVTVSGPDLAAHTG
                     HLFKGIVSVTCEITLVAAEATIRRALDPDIGLNLLALE"
     gene            complement(5250..5606)
                     /gene="cutA"
                     /locus_tag="AC16GM_07310"
     CDS             complement(5250..5606)
                     /gene="cutA"
                     /locus_tag="AC16GM_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR2923518.1"
                     /translation="MARRSAVRIDPHKFVLVLSTTAKQRVAERIARALVREKLAACVN
                     VIPGLISHYRWKGRVERSHEWLLLVKTRAALFPRLAERVKELHPYAVPEIISVPLAAG
                     SNDYLRWILEATMKER"
BASE COUNT         1226 a         1673 c         1649 g         1152 t
ORIGIN      
        1 ccgccgtggc tttccccatt tcgctcacaa ggtcgaagtc gctggttttg ggcaactccc
       61 tcaccgggcc ggggattaac ggtctcgata tcggagcgaa catgaacaaa ttgcagtaca
      121 atccggcgac ggtgagccag gacacggtgg tgggcaggtt caccaacgcg ctctccttcc
      181 agggcggcaa cgatttcggg gcgcaggcga atgtgaccaa ctgcgtcctc acgaacgaca
      241 tcggaagcgc cagcaccggg attctttaca acggggatac ggtgaaggtg agtttcacca
      301 aggacactgc cttcggtgcc tacggagcgg taatgaattc ccccggattt gtgctcacta
      361 ctgataactg catcttctcg gacacggcga tagcgacgaa gttcgtggac aacaacgcta
      421 cttccgtaac gctggatgcg cgctcgaact actggttctt cacggatacg agtacgattc
      481 ggggcgaact cgatggccca atcaacatca gaccgattaa caccacgtgc gagtctgggc
      541 cggggcagaa gcccgtgact ccagaggcat cgacacagaa gcccctggtc ttcgctcttt
      601 acgccaacgc gcccaacccg ttcaatccag cacgcgactg cagttctccc tgccggagcc
      661 gggggaagtt gaactggtga ttttcaatct tttggggcag aaggttagaa cagttgtcag
      721 gggtgcgtgg ctggcgggag tgcatgaagt gatctgggat ggaaaagatg accgaaacca
      781 gacgttagcg agcggcatat acttcgctcg acttcgggcc ggcgaaaacc agcaagtgca
      841 aaggatggtg atgctgaaat gaccgggaac aaagtgctct tgacgggtgt accgctgctg
      901 ttgtttgcct tggcgggtta tgcgttggcg ggacaggtta ccgtgccgct taccggttcg
      961 gcgcaggcgg ttgaaaacgg aacgccgcaa attcttttga agtttgacct cccgccggaa
     1021 ctggccaggg cgcacatcga ccacgcaacc ttgcgggtcg gtctcgtttc cgaagccaaa
     1081 gccgtgccgg tgcggcttct cctgataacc gacgactggt cgccctcggc caccgatttg
     1141 gtcgacccgc atattgccgt gaccgattcg ctttcgaccg gcaaggtctt gagcgccggt
     1201 gcggtttccc tggacgtcac cgacctagtg caggcactgg cggacggcgc gatgccaaat
     1261 cgagggttga tgctcgtccc cgttaaaccg ctgacgactt tccgattggg tgttgctcgg
     1321 gtgcaggtga gtatctttta cacgccggaa gagcagcact gaagacgcgt aaacaaaagg
     1381 caacggctcc tggaccattt agggggccgt tgccttacga tccgcagagt actacgattt
     1441 ttcagcaatc ggagtgacag aagcacagac tacggtttta atttaccggt cttgatccag
     1501 gcggccagat cagttatcac ttccggcgcc acccagcccg gcttcataat gtccaaaggc
     1561 gagcccgctt tatcctgggc gatgaaaagg tggttgagcg tcggatagac tttcccggtg
     1621 aacggctttt tggccgcggc gaaggcctgt tgccagccgg tgtagtcctg gtcggctaag
     1681 acctggtggt cgtagccgcc ctgcaagaat agaaagggga gtttggactg tgccgccgcc
     1741 tccgcctggt gcagagcaaa gaggtcgcgc cagtaactgg cgggggcgta gaagaacatc
     1801 gaatcgtcgg gcagggagcc gcttttgatc cgctcgaacc cctcccgcaa ctgtttctgc
     1861 tgaacagcga ttgaaaccga atcgaccccc tgggtctgca tcatcgtttt catctgccgc
     1921 agaagggtgg catcccacgg ctgggtcaaa cccgccagtg ctatcacccc ggccaatccc
     1981 ggcgcacgtc gggcaatgta gggtgccacg gtggcgccca gactgtggcc cagcaggaaa
     2041 actttggccg agtcaacctg cggaaagctt ttggccaggc gcagggctga aagcgcatcc
     2101 agaataacct catcttcgag aagtttgacc tgcgtatacc ccgcgcgcgt ccttttgtcg
     2161 tagcggagcg tggcaatccc ctgcttggcc agaccgtagg ccaagtcgcg gaacggttta
     2221 ttgggcccca ccgtttcgtc cttatcattc gggcctgagc cgtgaacgag cacaacgatc
     2281 ggaaatcctt tgcccgtgct gtccggcaaa gtgaggaggg cgggcaagcg caccgagtcg
     2341 gtcaccacct ccaccggctt ttcgaccata ccggcggagc ggaggtagtc gggcagcgcc
     2401 gaaggcgccg ggggagcctg ggcccaaacc accgctgcca gcagcagatg agtcagaacc
     2461 gccattacca gagcactgga aagacgtttg gtcatgctac tctccttgtt gtcccgaata
     2521 tagtcaatcc caaaggtatc gacagggaaa agtcttgtgt gggggaatga agactcaatt
     2581 gggtgtcaaa actcgtacgg tattacccaa cttccttcgg ccggtcaacc ggaccaccag
     2641 tatgctcagc tcgtagagaa aatagagcgg cacggccagc atcagctgcg aaaaggcatc
     2701 cggcgtgggc gtgagaatcg ccgctaagat gacgatgagg acgacagcgt aacgccgccc
     2761 tttcgcgagc gtccggtggg agatgatccc caattgcccc agaacgaagg ccagaacagg
     2821 cagttcaaac accgcgccga aggcgatcac catgaacgag acgaaactga cgtacttatc
     2881 gacggtgatc aagggctgga gtttggccga gccaaatgtc aggaggaatt tgatccccgc
     2941 cggcagcacc acgaagaagc agaaggcgcc gccccaggca aaaaagagcg tggcggcgac
     3001 gaccaccggc agcgccacct tcacttcgcg ctccagaagg ccggggacga cgaactgcca
     3061 ggcctggtag aggatcaccg gcacgcttaa aatcagcccg ccgtagagcg acagtttgat
     3121 tcgcaccgca aaggcttcgg tcacgcccga aaaatagacc gcccgtacgg gccgggtgag
     3181 gaaatccagc atttgatcgg agaaaaagta gatgacaact gagcccacca ggatgctggc
     3241 caggcacttg atgatgcgcc agcgaagttc ctccaggtgc gccagaaagg gcattgcttt
     3301 ggaatcacgc ggcatacggg gcgccgggct ttcaccgacg gggcggagat tttgagccgt
     3361 cggattgatc agaacggcca ccagggccta ggttaccaat cctttcccca actcccaaat
     3421 cctaatccct gactccgttt ctctcatttg ttcaagagtt tgttcaactc ctcctggaaa
     3481 tgctcgatgt cctgaaactt ccggtagacc gaagcgaagc ggacgtaggc aatgtcgtcc
     3541 agccccttta acttttccat caccttctcc cccacttcct ggcttgagat ttctcctttg
     3601 gagagattct gcagttcttc ctcgatttca tccaccaccg cgtcgatttt tttggcgctc
     3661 accggtcttt tgttgcaggc cagttcgatt ccccggcgca gtttctggcg gtcgaacggc
     3721 tcgcgccgtc cgtcgttttt caccaaggtg agttcccggt tctccacgta ctcgtaggtg
     3781 gtgtagcgct cgccgcaggc gaggcactcg cgccgccgcc tgaccgcgcg gccatcctgc
     3841 gcggtgcggg aatcgaccac cttatcctct tcgtgaccac aataggggca cttcatccag
     3901 agggcaaaat acacggggcg ggacgccccg tctacccaaa ctttggagaa gatgcacgag
     3961 ggggagacac cccatctacc cggaattgga gtcatccgga caggcgtatt gcgacgagag
     4021 tgccgacgtt tgcctgcagt ctttcagccg cgctcgcccg gttcagcgcc acttcgagaa
     4081 atcccgcact cccgattacc gctcccgcct tctttggagg aatcagtgca taggtttcaa
     4141 aaagtcccgc gagttcatgc ggcccggagt gaattttgaa ttgtcttctg gctactggct
     4201 cccggctacc ggctactttt tgcaggagcc attcccctgg gatgttcgtc gtgagatttc
     4261 cgaatctatc aacagagatg acatcgccgc gaatagcagc agcactccgg tgcggtttgg
     4321 gaatcgccag agattttatt cccttgacct ccggcccgaa ttcttccggc ttgaccccgc
     4381 gcgagaggta ggccgccacc ggcgcaaaaa tatcccgcgc gtgaaagacc ggtgactgcg
     4441 gattgagcca gaatcgtttc tctttgagat gaatgacttt cttgtggccg gaggcgggga
     4501 cgacgagcgc aaaaaggccg ttatccggcc caacgaaaaa gccgtggtcg gtgacaatca
     4561 aaagggcgcg ccgctttgag ccaactcccg gatcaaccac ggcaagatga atcgttcccg
     4621 gcggatagta tggcacaaaa cctttgagca tcagcgcacc cctcacaata ttgtggggtg
     4681 gtatctggtg gatagtatcg atgatacggg ctctcggatt gatggaagcg ataacgccct
     4741 tgactgccgg tacgtaggca tcggctgagc cgaaatcgct cgtgaaggtg accaaaggcc
     4801 cggtcgaatg cgactcgtgc cgagcggtca ttccagggcc agaagattca acccaatatc
     4861 gggatccagc gcccgccgga tggtggcctc ggccgccacg agggtgatct cgcaggtgac
     4921 tgagacgatg cccttaaaaa ggtgcccggt gtgcgcggcc agatccgggc cggagacggt
     4981 gacgtgggca tgcaccaccg gctttccgtc cagccaggcg atgttgccgg tgaggttccc
     5041 gatctcatac tcccccgggt actccttgcg cacgtactcc cgtgtgtgcg cgtcgaagta
     5101 gcccaactcc acgtcagcca ttgcccccaa gccggtgaaa ccgccacctt tgactttttc
     5161 ccgctcgaca aatccggcga gcgtagtgat gatctcctcc cccttcacca gccgcaccaa
     5221 gtaaccggaa ggaattcgct gaaattgcat taccgctcct tcatcgtcgc ttccaaaatc
     5281 cagcgcaggt agtcgttact tcccgccgca agaggaacgc ttatgatttc aggcactgca
     5341 taggggtgaa gttccttcac ccgctccgcc agccggggga aaagcgccgc ccgggttttc
     5401 accagaagca gccattcgtg tgatcgctcc actctccctt tccagcggta gtgggaaatc
     5461 aaacctggaa tgacgttcac gcaggccgcc agtttttcgc gcactaacgc tctggcaatc
     5521 cgttcggcca cgcgctgctt agcggtggtg ctcaaaacta agacgaattt atgcggatca
     5581 atgcggacgg cggaccggcg ggccatttac ccaactaagc gttcctgata gagcgggaaa
     5641 cggtcacaca ggcgggtgac ttcggccttc acctgccgga tggtatcctc gtcccccaga
//
LOCUS       DBNGZD010000272         1872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_667635, whole genome shotgun sequence.
ACCESSION   DBNGZD010000272 DBNGZD010000000
VERSION     DBNGZD010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_667635"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16GM_07315"
     CDS             <1..252
                     /locus_tag="AC16GM_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR2923519.1"
                     /translation="WYLKESEEYPILFFEGQPLSVDLPASVILRITATEPGVKGDSVA
                     NLTKNATLETGLVVKVPLFVKEGERIKVDTRTGDYLERA"
     gene            245..964
                     /gene="rnhC"
                     /locus_tag="AC16GM_07320"
     CDS             245..964
                     /gene="rnhC"
                     /locus_tag="AC16GM_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIR2923520.1"
                     /translation="MPDVESAERLTIRHDKSVVALYFPDTVTERKESLIGTDESGKGD
                     YFGPLVVAAVFWDERAAEALAGLRIRDSKLLSDRRAAVLAREIVRTTRHAVVIIGPEK
                     YNELIARMKNLNRLLAWGHARAIENILAAAACPRAVSDQFGDEKFLNNALLEKGRRIE
                     LVQKTKAESEPAVAAASVLARNTFLQYLDKLSERAGIPLPKGAGPPVDQAAQALVGKW
                     GKEALGQVAKLHFKNTARILG"
     gene            961..1200
                     /locus_tag="AC16GM_07325"
     CDS             961..1200
                     /locus_tag="AC16GM_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923521.1"
                     /translation="MNWQSELEKLCADNLSGSSALARKSGELLRSFARQESRHSGLEV
                     RKSFQSLAERLIRRFPEMASVEALLDQVALRLETK"
     gene            1243..>1872
                     /locus_tag="AC16GM_07330"
     CDS             1243..>1872
                     /locus_tag="AC16GM_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005851 - eukaryotic translation
                     initiation factor 2B complex [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="eIF2B alpha/beta/delta subunit family protein"
                     /protein_id="HIR2923522.1"
                     /translation="MSQFFRKQEIALAAIAEKAAAFFTKQVIVVTLSASMAVERMLIH
                     LHAEGKLAGLFVLESRPLGEGMLLAKRLAERGISVTAAVDAALDRVLEKADVALVGAD
                     CIDGQVVVNKIGTKLLALAARAQAKPCYVAADTSKFGAGLLTSTSETQGQPDEVAGDL
                     PAKVAVYNPYFEPTPLAWFTGIFSEQGLLTLLQVGEVCARRPQSGFVTAL"
BASE COUNT          395 a          534 c          577 g          366 t
ORIGIN      
        1 tggtacctga aggagtcgga ggagtacccg atcctcttct tcgaaggcca accgctgagc
       61 gtggatctgc cggcgtcggt cattttgcgc ataaccgcga ccgaaccggg ggtcaagggc
      121 gactcggtcg ccaatctgac caagaacgcc acgctggaaa ccggtctggt ggtgaaggtc
      181 ccgctctttg tcaaagaagg cgaaaggatc aaagtcgaca cccggaccgg cgattactta
      241 gaacgtgcct gacgtcgagt ccgccgagcg cttgacgata cgccacgaca aatcagtcgt
      301 ggcgttgtat tttccggata ccgtgaccga gcgaaaagag agtctcatcg ggaccgacga
      361 atcgggcaag ggcgattact tcgggcctct ggtagtggca gcggtcttct gggatgagcg
      421 ggctgccgaa gctttggcgg gactgcggat cagagattcc aaactgcttt ccgaccgccg
      481 cgctgcggta ctggcccgcg agattgtccg gacgacccgg cacgccgtgg tgatcatcgg
      541 accggagaaa tacaacgaac tgattgcccg gatgaaaaat ttgaaccgcc tcctggcctg
      601 gggacacgcc cgggcgattg agaacattct ggcggctgcc gcctgccccc gggccgtcag
      661 cgaccagttc ggcgatgaaa agtttcttaa taatgccctg ctggaaaaag gacggcggat
      721 cgaactggtc cagaaaacga aagccgagag cgagccggca gtcgccgcgg cttcggttct
      781 cgcgcgcaac acttttctcc aatatctcga taaactttcg gaacgggccg gaattcccct
      841 ccccaaaggg gccggcccgc cggtcgacca ggcggcgcag gcgttagtgg ggaagtgggg
      901 aaaggaagcg ctggggcagg tcgccaaact tcacttcaaa aataccgcgc gcatccttgg
      961 atgaactggc agagcgagtt agaaaaactt tgcgccgata atctttccgg ttccagcgcg
     1021 cttgcccgga aatcgggcga actgctccgg tccttcgccc gtcaggaaag ccggcattcc
     1081 ggcctcgagg tcaggaaaag cttccaatcc ctggccgagc ggctcatccg ccgttttccc
     1141 gaaatggctt cggtggaggc gctgctcgat caggtggccc tgagactgga aacgaaatga
     1201 tggaatagtg cctttgacct cacccgactc gtcgccaccg aagtgtcgca atttttccgc
     1261 aaacaagaaa tagccctggc cgctatcgcc gaaaaagcag cggccttctt caccaaacag
     1321 gtgatcgttg tgactttgag tgcttctatg gcggtagaac gaatgctcat tcatttgcat
     1381 gcggagggca agctggcggg tctgttcgtt ctggaatcac gaccgctggg cgaggggatg
     1441 ctcctggcca aacggctggc ggagcgaggc atttcggtca cagcggccgt cgatgccgcg
     1501 ttggaccggg tccttgaaaa ggcggatgtg gcgctggtcg gcgccgattg cattgacggg
     1561 caggtcgtgg tcaataaaat cggaacgaaa cttttggccc tggcggcgcg ggctcaggcc
     1621 aaaccctgct acgtcgcagc cgacacgagc aagtttggcg ccggtctttt gacctctacc
     1681 agcgaaacgc aagggcaacc cgatgaagtg gccggtgatc tgcccgccaa ggttgccgta
     1741 tacaacccct attttgagcc aacgcctctg gcctggttta ccggaatctt ctctgaacag
     1801 ggactcctca ctttgcttca ggtgggtgag gtctgtgccc ggcggccgca aagcggtttc
     1861 gtaactgccc tc
//
LOCUS       DBNGZD010000273         5083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1294437, whole genome shotgun sequence.
ACCESSION   DBNGZD010000273 DBNGZD010000000
VERSION     DBNGZD010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1294437"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..368
                     /locus_tag="AC16GM_07335"
     CDS             30..368
                     /locus_tag="AC16GM_07335"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR2923523.1"
                     /translation="MAYCPKCQAEYKDGRDECIDCGVPLVEAMDELEETEDSGGFVPL
                     RTLPSRMYAQMLKESLEKAGIPALIKGEDIGIMLGNFGTTSTVPVELWVPADEVDKAE
                     AIAAGMMGEK"
     gene            476..730
                     /locus_tag="AC16GM_07340"
     CDS             476..730
                     /locus_tag="AC16GM_07340"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR2923524.1"
                     /translation="MSAAFVLLRRVESRPEALVIQGVLETAGIPVVVRTPDKYLLLQG
                     DWSGSPKPPPVEIWVAGERKAEAEELLKSPSGLTDDTSSI"
     gene            1043..4177
                     /locus_tag="AC16GM_07345"
     CDS             1043..4177
                     /locus_tag="AC16GM_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HIR2923525.1"
                     /translation="MIFGGKKVTFSYQMLKNYVSRVFKVSQRGDAREESYYSALESLL
                     RDYAESVGKRGTDVTTLPKKTEGGNPDFRVWDGKEKIVGYIEAKDPSVAHLDQIETSE
                     QLKRYLGIFPNLILTNFHEFRLYRNGDLIDQVLIARPVIGRKLKVVPPVEREEKFLQL
                     LEKFFSFSLPAITDAKTLAEELAKRTRFLRDEIISEELKAGKGRVAGFYEAFKKYLIG
                     GLTKEDFADLYSQTITYGLFASRTRSENGFNRRLAYYNIPATIGILRDVFKYISLEDP
                     PQQMEWIIDDISQILANTKVNDILHRYFHEGKGKDPIIHFYETFLTEYDPDTRERRGV
                     YYTPEPVVSYIVRSLHKILKEEFNRPDGFASTSVTVLDPAAGTLTFLAEAAKVAVDEF
                     VEKYGEGGKQNLIKEHILKNFYAFELMMAPYAVGHLKMGFLLEELGYKLKKDDRFKLY
                     LTNTLEMEELTQTDLPGMASLSEESHLAGKVKKETPILVILGNPPYSAVSANMGEWIM
                     GLIEDYKYVDGKHFGERKHWLQDDYVKFIRFAQWKINQAGEGVLGFITNHSYLDNPTF
                     RGMRQSLMKSFDEIYVLDLHGNSLKKEKCPDGAKDENVFDIQQGVAISLFVKRRSKVK
                     NVRIYHSEFWGLRENKYDSLKRVDIKSISWQELAPLTPFYFFVPRKEKGIEIYQHYCN
                     IMDIFLSSGTGIFTGKDHFVVDFAKEPLKARVLMFMNKNLSNEMVKQGLNLKENYMWR
                     VAKARKDLYKDENWQEYFKEILYRPFDIRDIYYHDSVVWRPRKEIMRHLLRENLALCV
                     GRAGQVVGLEKPWNIVFISKNIVDLNLFYRGGEQVFPLYVYFDQDKKDLFSYTKKSEK
                     RKPNVKPEIFKNLASAFKKEPTPEEIFYYIYAVLYSNTYRTKYAEFLKIDFPRIPFTN
                     DYKLFSKMGEYGKQLVDLHLLRFNFQLIAKFPVKGDAVVEKLRYDEKQKCVYINKEQY
                     FEGIPKDVWEYQIGGYQVCEKWLKDRKGRRLSLEEIKHYCNIVTALQKTIEVQKEIDA
                     IYPQVEQDVIQFGK"
     gene            4529..4927
                     /locus_tag="AC16GM_07350"
     CDS             4529..4927
                     /locus_tag="AC16GM_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR2923526.1"
                     /translation="MTKFYNKSSEKEKRSRLRNQMPAAEVILWSRLQKRQILGFKFRR
                     QYSVGPYVLDFYCPEVKLAVEIDGDSHFREDASEYDDNRQMSIEELGVRFLRFTNLEV
                     YKNLTGVLKSITGTIEKVGKQAGRKTKRGK"
BASE COUNT         1469 a         1082 c         1319 g         1213 t
ORIGIN      
        1 cagcgggatg agcaggtgga ggattggcaa tggcgtattg tcccaagtgc caggcagagt
       61 acaaagacgg ccgcgacgag tgcatcgact gcggcgtgcc gttagtcgag gcaatggacg
      121 aattggaaga aaccgaggac agcggcgggt ttgtgccgct gcggaccctg ccctcccgca
      181 tgtacgcgca gatgctgaaa gagtcgctgg agaaagcggg cattccggct ctgatcaaag
      241 gcgaggacat cggcattatg ttagggaatt tcggcaccac ttccaccgtg ccggtggaac
      301 tctgggtgcc ggcggatgaa gtggacaaag ccgaagcgat tgccgccgga atgatggggg
      361 agaagtaagc cgggtttatc cgatcggaat attgccccgc atttcgtatt caggaagctc
      421 ttgcagttcg ccactcaact ccatttccat ttgacaattt tttgagacga ggaaaatgtc
      481 agcggcattc gttttgttgc gccgggtgga aagccgtccc gaagcgttag tcattcaggg
      541 cgtcttagag accgccggga ttccggtagt cgtgcggaca cccgataaat accttctcct
      601 gcagggcgat tggagcggtt cgcccaaacc gccgccagtg gaaatctggg tggcggggga
      661 gcggaaagcc gaagcggaag agctcctcaa atccccaagc ggattgaccg acgacaccag
      721 ttctatttag tgccttcttg cggggtgcgg tagacaacga gccgagcggc tggaagtgga
      781 aagtggagta gggaaagggt cgttgaaaac cctgccatcg gctctgaacg agagcaaaac
      841 cgaagggagg tttacgctgg tgctgccggt gtaaaagcaa aacaccccct gggcctaaag
      901 gccccgtccc ccttgccaag ggggacataa aaagcaaaac aggaattttg tccccctttg
      961 tttaaggggg acgccacgcc aaaggcatgg cagggggtag tgtccgcgcc ggtggctgaa
     1021 aactggtgcg gaactggcgg gattgatttt tgggggtaaa aaggtaacat tttcctacca
     1081 gatgttaaaa aactacgttt cgagagtctt caaggtcagc cagcggggag acgccagaga
     1141 ggaaagctat tattccgccc ttgaaagtct gttaagggac tatgccgaat cggtcggcaa
     1201 aaggggaact gacgttacca ccctgcccaa gaaaaccgaa ggaggaaatc ctgattttcg
     1261 ggtgtgggac gggaaagaga aaatcgtcgg ttatatcgaa gccaaggatc cttccgttgc
     1321 ccatttagac cagattgaaa ccagcgagca gttgaagcgt tatctgggta ttttccccaa
     1381 ccttatcctg accaatttcc acgaattccg gctttatcga aacggcgatt taatagatca
     1441 ggtgcttatt gcacggcccg tcatcggtcg gaaattgaaa gtcgtcccgc cggttgaacg
     1501 ggaagaaaaa ttccttcaac tactggagaa gttcttctct ttttctcttc cggcaatcac
     1561 cgatgccaag accctggcag aggaactggc gaaaaggaca aggtttttgc gggacgagat
     1621 tatttccgag gaattaaaag cggggaaggg acgagttgcc gggttttatg aggcattcaa
     1681 aaagtactta atcggcgggt tgaccaaaga ggattttgcc gacctgtatt cgcagaccat
     1741 tacttacggc ctttttgctt cacgcacccg ttcggaaaac gggttcaacc gccggttggc
     1801 ctactataac attccagcaa ctattgggat cctgcgggat gtttttaagt acatttctct
     1861 ggaagatcct ccccagcaaa tggaatggat tattgacgac atttcacaaa ttctggcaaa
     1921 tacaaaggtc aatgacatcc ttcaccggta ttttcacgag ggaaagggca aagacccgat
     1981 cattcatttt tatgaaacct ttctgacgga atacgacccg gatacgaggg aacggcgcgg
     2041 ggtttattac acgccggagc cggtggtctc ctatatcgtc cgttctttac acaaaatttt
     2101 gaaagaggaa ttcaacagac cggacggatt tgccagcacc tccgttaccg ttttggaccc
     2161 cgccgctgga acgctgacct ttttggccga agcggctaag gtggccgtgg atgaatttgt
     2221 cgaaaaatac ggtgaaggcg gtaaacagaa tctaatcaaa gaacatatcc tgaaaaattt
     2281 ctatgccttt gaactgatga tggctcccta tgccgttggg catttgaaga tgggttttct
     2341 tttggaggaa ctggggtaca agctgaaaaa agacgaccgg ttcaagctat atctgacaaa
     2401 tacgctggaa atggaagagc taacccaaac cgacctcccc ggaatggcct ccctttcgga
     2461 agaatctcat ctggcgggta aggttaaaaa agaaacgccc attctagtaa ttttaggcaa
     2521 tcctccatat tctgcggtat ccgcaaatat gggtgaatgg attatggggc ttattgaaga
     2581 ttataagtat gtagatggga agcatttcgg ggagagaaaa cattggctac aagatgatta
     2641 tgtgaaattt atccgctttg ctcaatggaa aatcaaccag gcgggagagg gggttttggg
     2701 atttatcaca aaccacagct acttggataa ccctactttt cgcggaatga gacaatcatt
     2761 aatgaaaagt tttgatgaaa tctatgttct ggatttacac ggaaatagtt tgaagaaaga
     2821 aaagtgcccc gacggtgcta aagatgaaaa tgtttttgac attcaacagg gagttgctat
     2881 ttcgcttttt gtaaagagaa gaagtaaagt aaaaaacgtc agaatatatc attccgaatt
     2941 ttggggacta agggagaaca agtacgattc tcttaagaga gtggatataa aaagcattag
     3001 ctggcaagag ctagctcctt tgaccccttt ttatttcttt gttccaagga aggaaaaagg
     3061 gatcgaaatt tatcaacatt attgcaatat catggatatt ttcctctcaa gcggaacagg
     3121 catctttaca ggtaaagacc attttgttgt cgattttgcg aaagaacctc tgaaagcccg
     3181 cgtcttgatg tttatgaaca aaaatctttc aaatgaaatg gtaaagcaag ggttgaatct
     3241 taaagagaat tatatgtgga gggttgctaa agcgagaaaa gatttatata aggatgaaaa
     3301 ttggcaagaa tatttcaagg agattctata cagaccgttt gatattaggg atatctatta
     3361 ccacgattct gttgtttggc gtcctcggaa agaaataatg aggcatttac tccgggagaa
     3421 tttggcatta tgtgttggta gagcaggcca agtggttggt ttagaaaagc catggaatat
     3481 tgtatttatt tccaagaata ttgtggattt gaatctgttt tatcgcggtg gagaacaagt
     3541 ctttccgcta tacgtctatt ttgaccaaga caaaaaagac ctcttcagct acaccaaaaa
     3601 atcggagaaa agaaaaccga acgtaaaacc ggaaattttc aaaaacttag cgagtgcttt
     3661 taagaaagaa ccaacacccg aagaaatttt ttattacatc tatgcggttt tgtattcgaa
     3721 tacctaccgc accaaatacg ccgaatttct aaaaattgat tttccgagaa ttccgtttac
     3781 caacgattac aaattgttct ctaaaatggg ggaatacgga aaacaactgg tggatttgca
     3841 ccttttgcgt ttcaatttcc agctaatcgc caaattcccg gtcaaagggg atgcggtggt
     3901 ggaaaaattg agatacgacg aaaaacaaaa atgcgtttac ataaacaaag aacaatattt
     3961 tgaaggaatc ccaaaggatg tctgggaata tcaaatcggc ggttatcaag tttgcgaaaa
     4021 atggctgaag gacaggaaag gtagaagatt gtcgttggaa gaaatcaaac attattgcaa
     4081 catcgtcacc gctctgcaaa agaccattga ggttcagaaa gagattgacg cgatttatcc
     4141 ccaagtcgaa caggatgtaa ttcaattcgg gaaatagaaa aacgaaggga caggccgacg
     4201 tgactgctcc gggcgggcac gaggttcgtc cccgtgaccc cctgcggcct gaaagccgcg
     4261 tcccccttac gtcaggggga caaagtgacc ccctgggcct gaaagccgcg tcccctttac
     4321 gtcaggggga caaagtgacc ccctgggcct aaaggccccg tcccccttgg caagggggac
     4381 agaaatagca aagcagaaac tttttgttcc ccttttttca agggggacgc acagggggtc
     4441 gagttttcct ccttacttaa gggcgaccca cagggagtag tttttgctcc tttgaagaga
     4501 gggactttag agggtatatt ttaactgaat gaccaaattt tacaataaat cttccgagaa
     4561 ggagaagcgc agccggctgc gaaatcaaat gcccgcggca gaggtcattc tctggtcgcg
     4621 gctccagaaa agacagattt tgggattcaa attccggcgg caatacagcg tcgggccgta
     4681 cgttttggat ttctactgcc cggaagtgaa attagcggtt gaaatcgacg gtgacagcca
     4741 ttttcgagaa gacgcctccg agtatgacga caaccggcag atgtcaatcg aagaacttgg
     4801 agtacgattc ctccgtttca ccaacctcga agtttacaaa aatcttaccg gcgttttgaa
     4861 gtccataacc ggaacgatcg aaaaagtcgg aaagcaggcg gggagaaaaa caaaaagggg
     4921 aaagtgaccc cctgggccta caggctgtgt ccctcttaat tagaaggaca aaccgacccc
     4981 ctcggcctaa cggccgtgtc ccccatgtaa aatgggaaag aaatatcaaa atagaaaatt
     5041 tttttacccc ttttttaaat ggggaagcac cgccaaacgc ggg
//
LOCUS       DBNGZD010000274         1855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1225523, whole genome shotgun sequence.
ACCESSION   DBNGZD010000274 DBNGZD010000000
VERSION     DBNGZD010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1225523"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC16GM_07355"
     CDS             complement(<1..227)
                     /locus_tag="AC16GM_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2923527.1"
                     /translation="MTLLKKILAASILGLTFSTPAGAKDNCLECHGQLDGNLQAPAVG
                     ITDDIHYQRGISCAGCHGGDSKEEDADLSMNP"
     gene            complement(232..1311)
                     /locus_tag="AC16GM_07360"
     CDS             complement(232..1311)
                     /locus_tag="AC16GM_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR2923528.1"
                     /translation="MPFGSGFYRWLGERINLQPLLHLVKKKAVPAHRHSVWYYTGGMT
                     LFFFGVQVVTGILLLLYYRPSAEAAYESVQFIMTEVKFGWLVRSIHSWSANLMIGALF
                     VHMFSTFFMKAYRKPRELTWFSGAALLFLALCFGFSGYLLPWNKLAFFATKVGTEIAG
                     QLPGIGHSLLVFLRGGEEVSGATLTRFFGFHVAILPGVVTFLLLLHLLLVQVQGMSVP
                     IRVAKNPTYRTVPFFPNFLLRDLLGWLLALLLLVVLASIFPWELGEKADPLAPAPAGI
                     RPEWYFLFMFQTLKLIPAKILSIPGEFLGILAFGAGGILWFLVPLLDKSPEGKSGRWL
                     FRLGWVLVAYMAVLTVLGYLWPAGK"
     gene            complement(1313..1753)
                     /locus_tag="AC16GM_07365"
     CDS             complement(1313..1753)
                     /locus_tag="AC16GM_07365"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR2923529.1"
                     /translation="MAENKSRREFLNFILGGGLATLLGAILYPILRFVMPPAKSEAFG
                     GRVTAAKVGELAPNSGKIFPLGDTPGILVQTAGGEYKAFSAVCTHLSCTVQYRPDLGE
                     IWCACHNGHYDLSGKNIAGPPPRPLTEYPVTVAGDNVVVTQRGV"
BASE COUNT          454 a          533 c          528 g          340 t
ORIGIN      
        1 gggttcatcg aaaggtctgc gtcttcttct ttcgaatcgc ccccgtggca gccagcgcac
       61 gaaatccccc gctggtagtg aatgtcatcg gtgatgccga cggctggcgc ctggagattg
      121 ccgtctaatt gcccgtggca ctccagacag ttatccttcg ctcccgcggg agtggaaaac
      181 gtcaagccca aaatgctggc tgcgagaatt tttttcagca gagtcatttt tttatttccc
      241 cgccggccag agatacccca gaaccgttaa gacggccata taggcaacca gaacccagcc
      301 gagccggaaa agccaccgcc ccgattttcc ttcgggagat ttatccagga ggggaaccaa
      361 aaaccacaga attccccccg caccaaaggc caaaattcct aaaaactccc cggggataga
      421 taagattttg gccggaatca acttgagggt ttgaaacata aagaggaagt accattccgg
      481 ccggatgccc gccggcgccg gagccagagg atccgccttc tcccccagtt cccaggggaa
      541 aatggaagcc agaacgacta agagcagaag cgccaaaagc cagcccaaca gatcgcgcag
      601 aagaaagttt gggaaaaaag gaacggtgcg gtatgtcgga ttcttggcca cccgtatggg
      661 gacgctcata ccctgcacct gcaccagcag gagatgcagg agcaaaagaa aggtgaccac
      721 tccgggcaaa atggcgacgt gaaagccgaa aaagcgcgtc agggtggcgc cggaaacctc
      781 ttccccgccc cgcaggaaaa ccaagagact gtggccgatt cccggcaact gcccggcgat
      841 ctcggtgccg actttggtgg caaagaaggc cagtttgttc cagggcaata ggtagccgga
      901 aaaaccgaag cagagtgcca gaaagagaag agcggcgccc gagaaccagg tcagctcgcg
      961 cggcttacgg taggccttca tgaaaaaagt tgagaacatg tgcacgaaga gggcgccgat
     1021 catgagattg gccgaccagg agtgaatcga acgcaccagc cacccgaact tcacttcggt
     1081 catgatgaac tgcaccgatt cgtaggccgc ctcggcggag ggtcggtagt agagcaaaag
     1141 cagaatgccg gtgaccacct gcactccgaa gaaaaagagc gtcatcccgc cggtatagta
     1201 ccagaccgaa tgccggtggg ccggcaccgc tttctttttg accagatgca gaagtggctg
     1261 gaggttgatc cgctccccca gccaccggta aaagccggaa ccgaaaggca cgctacacac
     1321 ccctttgggt cacgacgacg ttgtctccgg ccaccgttac cggatactcg gtcagggggc
     1381 ggggcggcgg cccggcaatg tttttaccgg agaggtcgta atgcccgttg tggcaggcgc
     1441 accagatctc ccccagatcg ggccggtatt gcacggtaca ggaaaggtgg gtgcagactg
     1501 ccgaaaaggc tttgtactcg cctcccgcgg tttgcaccag aatgccaggg gtatcaccca
     1561 gaggaaagat cttccccgaa ttcggcgcca gttccccaac tttggctgcg gtgaccctcc
     1621 cgccgaacgc ttccgacttt gccggcggca ttacaaagcg gagaatcggg tagaggatgg
     1681 ctcccaataa cgtagccaat cctccaccca gaatgaagtt cagaaactcg cgacgcgatt
     1741 tgttctcggc catgcctccg tgactaattc gctttcggcg gcttcttatg ctcgatcttt
     1801 ttgtacgcct cggcgtacac gaacggcttg ttgaacggat tttccgggtt gttgt
//
LOCUS       DBNGZD010000275         1471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_948074, whole genome shotgun sequence.
ACCESSION   DBNGZD010000275 DBNGZD010000000
VERSION     DBNGZD010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_948074"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="AC16GM_07370"
     CDS             <1..928
                     /locus_tag="AC16GM_07370"
                     /inference="COORDINATES: protein motif:HMM:NF022007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR2923530.1"
                     /translation="GDCTVSGAVGTAITVEYREDDSLLCLLALAGGLTEQADSSRVEI
                     TRLSNPHGPETFTIDLRAEQPDRAAVPGDVILVRTNPQILRQYAVWVSGEVVSPGSYV
                     IDPGQTKLSEVIQRAGGFTPRAALAQAAILRPAGLSGSRSSSDTLPTAFLSQLSPEEI
                     DYYKTRVLLRPGQVSCDFVRIFERGDLAADVIVQEGDYITVPSRSPSVYVTGRIAQPG
                     FVAHEPDRPLRYYIERAGGYSWNADPGKARVIKGVSGAWQKASPDTRVDAGDTIWIPE
                     KKKQKFLTAMRDNLLLLGNLTTIYLVVKQATK"
     gene            997..>1471
                     /locus_tag="AC16GM_07375"
     CDS             997..>1471
                     /locus_tag="AC16GM_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIR2923531.1"
                     /translation="MWAFLSLVFRQRRLIAVNFIIVCLATVGVTMLLPNWYRATTTLL
                     PPEKETFNLNLQGTMIAGFPSLGGGFALPFTASPSDVLSAILKSRTVAEAVVKQNQLQ
                     ERWKTKSLSDAIEKVQRRLAVSVEPQGVISLSFESRNPQEAAEVANSFIVELDQVN"
BASE COUNT          299 a          447 c          437 g          288 t
ORIGIN      
        1 gggcgactgc accgtttccg gagccgtggg cacagccatc accgtcgagt accgggaaga
       61 tgactcgctc ttatgtcttt tggcgctggc cgggggctta accgagcagg ccgattcgag
      121 ccgcgtagaa attacccgct tgagcaatcc ccatggtcca gagacgttca ccattgacct
      181 ccgggcggaa caacccgatc gggcagctgt gccgggcgac gtcattttgg tccgaacgaa
      241 tccacagatc ctccggcagt acgcagtgtg ggtcagcggg gaagtggtgt ctcccggaag
      301 ctacgtcatc gacccggggc agaccaaact ctctgaggtg atccagcgag ccggaggatt
      361 caccccgcga gcggccctgg cgcaggccgc cattctgcgg ccggccggcc tgtccggctc
      421 ccgcagcagc agtgacaccc tgccgaccgc tttcctgagc cagctctcac ctgaggagat
      481 tgattactac aaaacccgcg tgcttttgcg tccgggacag gtctcctgtg attttgtgcg
      541 gatatttgaa agaggcgacc tcgccgctga cgttattgtc caggaggggg actacatcac
      601 ggttccgagc cggagtccct cggtgtacgt cacgggtcgg atcgcgcaac ccggttttgt
      661 ggcccacgaa cccgaccgcc cgctgcgcta ctacattgag cgcgccggag gctatagctg
      721 gaatgccgac ccgggaaagg cgcgagtcat caagggggtc tcgggtgcct ggcaaaaagc
      781 cagcccggac acccgggtcg atgcgggaga taccatctgg attcccgaga aaaaaaagca
      841 gaaattcctg accgccatgc gagacaatct gctcctgctg ggtaacctga ctacgatcta
      901 cctggtggtc aagcaggcaa cgaaatgact tcgccccggc tgattcccga agatgcaggg
      961 ttagacctgc ctcccggtca ggaaagggag gtcagtgtgt gggcttttct ctccctcgtc
     1021 ttccgtcagc ggcggctcat cgcagtcaac tttatcatag tttgtttggc caccgtcggc
     1081 gtcacaatgc tattgcccaa ttggtaccgg gcgaccacga ccctgctccc tccggagaaa
     1141 gagaccttca acctgaacct gcaaggcacc atgatcgcgg ggtttccctc cctgggcggc
     1201 ggtttcgcct tacctttcac cgcctctccg tcggacgtgc tctcggctat cctgaaaagc
     1261 cgcaccgtgg ccgaagcagt ggtaaagcaa aaccagctcc aggagcgctg gaaaaccaaa
     1321 agtctctcgg atgcgatcga gaaagttcaa cggcggctgg cggtttccgt agagccgcag
     1381 ggggttatca gcctttcctt tgaatccaga aatccgcagg aggcggcgga ggtggccaat
     1441 agtttcatcg tggaattaga ccaggtcaac c
//
LOCUS       DBNGZD010000276         2884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1365040, whole genome shotgun sequence.
ACCESSION   DBNGZD010000276 DBNGZD010000000
VERSION     DBNGZD010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1365040"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="AC16GM_07380"
     CDS             <1..1084
                     /locus_tag="AC16GM_07380"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR2923532.1"
                     /translation="GPGQGFLATFQPQGIKALYTGRSGQWVRLKLAEGQEAWTSQDKI
                     RFLPPGTPPPTSTVTLIRVFEEARAVRIELPLSEKLPFRVDEELDPPQQRLTVYGLTS
                     AVDWIRQYLESDLVRLVSWEQPQKGVFRLTCELKPGQLWGYDVSYEKNTLVWVINKQP
                     AWLGRLKGLVFTIDPGHSADQGALGPTGLEERQANLWLAIKVADLLRKKGATVVMTRT
                     GMEDVPLMERPKIAKKAGTHIYLSIHNNALPDGVNPFFNNGTSVYYYHPHSRALAEAI
                     HKRMVAGTGLPDFGLYYANFAVDRPTQYLAVLVECAFMMIPEQEEKLRSEAFQEKVAS
                     LIVAGVEDFLLSVKKKPQTKAPESPR"
     gene            complement(1124..1945)
                     /locus_tag="AC16GM_07385"
     CDS             complement(1124..1945)
                     /locus_tag="AC16GM_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR2923533.1"
                     /translation="MPRRTAKIFWTALLSLLLLTLLFPLGWMVVTSFSAPGGSGGLAA
                     TFLQGKWTLANFRDVFSQKIFLRPLLNSLFVTALITIGNLIFCLSVGYAFARYRFRGK
                     GVLFAAVLGVLMIPQHIVIIPLFLLMQKFGWYDTYFALVIPWLVNPLGVFLLRQYIEN
                     LPRDLEDAARLDGAGILRILYTVVAPLCKPALAVLAIQIFLVVWNWFLFPFILTSSET
                     LRTLPVALALYQGYQTIDWPHLMAASTLATLPVVAVFLFFQRQIISGITAGALKQ"
     gene            complement(1947..2813)
                     /locus_tag="AC16GM_07390"
     CDS             complement(1947..2813)
                     /locus_tag="AC16GM_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR2923534.1"
                     /translation="MRARSQPYLLASPWILTLLLFWLFPLVWSLYLSFTNYGILNPQS
                     DWIGLANYQKLFRDPAFWIALKNTFIFVIGTVPVTTVLALAFALALNQKLPWRGLFRA
                     GFFIPSITSLVVVALVFTNLYSQNGYLTLLAKAAGIETRGLLLSEKTALPAIMLMDVW
                     AAVGYYLLLFLAALQAIPQELYEQADISGASRWQKFRHVTLPHLRPMFLFVVVINAIK
                     SFQVFTEIFVMTKGGPLSATTTSVYQVYEEGLQRFQMGYASALAYLLFLIIAGFSFLA
                     ARLFRLGKGIAD"
BASE COUNT          726 a          777 c          873 g          508 t
ORIGIN      
        1 cggcccgggt caggggtttc tggccacctt tcagccgcag ggtatcaaag cgctctatac
       61 cggcagaagc gggcagtggg tcaggttgaa attggccgaa ggccaggagg cttggacgag
      121 tcaggataaa atccgcttct tgccgcccgg cacgccgccc ccgacttcga cggtcaccct
      181 catccgggtc tttgaagagg cgcgcgcggt gcgcattgaa ctgcctctgt ccgaaaaact
      241 gccctttcgc gtcgatgaag aactggatcc gccgcaacaa cgcttgactg tctacggact
      301 cacctcggcg gttgactgga tcagacagta cctggagagc gatctggtcc gcctggtctc
      361 ctgggagcag ccgcagaaag gggttttccg cctcacctgc gaattgaaac ccggtcagtt
      421 gtggggctac gacgtttcct acgagaagaa caccctggtc tgggtgatca ataaacagcc
      481 ggcctggctc ggccgcttga aaggtctggt ctttaccatc gatcccggcc actccgccga
      541 tcagggcgcc ctgggcccca ccggtctgga agaacggcaa gccaatctct ggctcgccat
      601 caaagttgcc gaccttttgc gcaaaaaagg agccacggtg gtaatgaccc gcacggggat
      661 ggaagacgtg cctttgatgg agcggcccaa aatcgccaag aaagcgggaa cgcacattta
      721 cctgagcatc cacaacaatg cactgcccga cggggtgaat cccttcttca acaacggcac
      781 cagcgtctac tactaccacc cgcacagccg ggcgctggcc gaagcgattc acaagcggat
      841 ggtggcgggc accgggttgc ccgacttcgg gctctactac gccaatttcg ccgtggatcg
      901 tccgacgcag tacttagcgg ttttggtgga atgcgccttc atgatgattc cggaacagga
      961 ggaaaaattg cggtccgaag ctttccagga aaaagttgca tcgctgatcg tagcgggagt
     1021 agaagatttc ctgctttcgg taaagaagaa accgcagacg aaggcgccgg agtctcctcg
     1081 gtgaaatatt aattgaccag taggggttca acgcgttgaa cccctactgc ttcaacgccc
     1141 ccgccgtaat ccccgaaata atctgccgtt ggaagaaaag gaaaaccgcc accacaggca
     1201 gagtcgccag tgtagaggcg gccatcaggt gcggccagtc gatggtctgg tacccctggt
     1261 agagggccag ggccaccggc agggtgcgga gcgtttcgct ggaggtgaga atgaaaggga
     1321 agaggaacca gttccagacc acgaggaaaa tttgaatcgc caagaccgcg agggccggtt
     1381 tgcacaaagg cgccactacg gtgtagagaa tccgcaaaat gccggcgccg tcgagccggg
     1441 cggcatcttc gagatcgcgc gggaggtttt cgatatactg tcgcaagagg aaaacgccca
     1501 gcggattcac cagccagggt atcaccagtg cgaaataggt gtcgtaccag ccgaacttct
     1561 gcatcaaaag aaagagggga atgatgacga tgtgctgggg aatcatcaag accccaagca
     1621 cggcggcgaa gagcacccct ttgccccgga accggtagcg agcaaaagca tacccgactg
     1681 agaggcagaa aatcaggttg ccgatggtaa tcaaggcggt gacgaaaagg gaattcaaaa
     1741 gcgggcgtag aaaaattttt tgtgaaaaga cgtcacggaa attggccaga gtccatttgc
     1801 cttgcaagaa ggtcgctgcc aaaccgccgg agccccctgg cgcagaaaaa gaagtcacca
     1861 ccatccaccc caaagggaag agcagggtga gcaaaaggag gctcaagagc gcagtccaga
     1921 agattttcgc ggttcggcgg ggcatctcag tcggcaatcc ctttccccag ccgaaacaag
     1981 cgcgcggcca gaaaactgaa accggcaatg atgaggaaca ggaggtaagc caaggccgaa
     2041 gcgtacccca tctggaagcg ctgcaggccc tcttcgtaga cttggtagac ggatgtcgtc
     2101 gtggcggaga gcggcccgcc tttggtcatg acaaaaattt ccgtgaatac ctggaacgat
     2161 ttgatggcgt tgatcaccac cacgaagagg aacatcggcc gcaggtgggg cagggtgacg
     2221 tgccgaaatt tctgccagcg gctggcgccg gaaatatcgg cctgctcgta gagttcctgc
     2281 ggaatggcct gcagcgcggc caaaaacaaa aggaggtagt agcccaccgc ggcccagacg
     2341 tccatcagca taatcgccgg cagggcggtc ttttccgaga gtaaaagacc gcgagtttcg
     2401 atgccggccg ctttggccag aagcgtcagg tagccgtttt ggctgtaaag gtttgtgaaa
     2461 accagcgcca ccaccacgag tgaagtgatt gaggggatga aaaatccggc ccggaaaagt
     2521 ccgcgccagg gcagtttctg attcaacgcc aacgcgaagg cgagcgctaa aaccgtcgtt
     2581 accgggacgg tgccgatgac gaagatgaac gtatttttca acgctatcca gaaggccgga
     2641 tcgcggaata acttctggta gttggcgagg cctatccagt cggattgcgg attgagaatg
     2701 ccgtaattgg tgaaactcaa ataaagtgac caaaccaggg ggaaaagcca gaacaaaaga
     2761 agcgtcagaa tccagggaga agctaagagg tagggctggc ttcgggcccg cacgtcacct
     2821 cacggagatt tcaaaagcgc gtcaattgtc gcgctcgcgt cgactaaggc ctgccggggt
     2881 gact
//
LOCUS       DBNGZD010000277         1706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1504038, whole genome shotgun sequence.
ACCESSION   DBNGZD010000277 DBNGZD010000000
VERSION     DBNGZD010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1504038"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="AC16GM_07395"
     CDS             <1..803
                     /locus_tag="AC16GM_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2923535.1"
                     /translation="AGDSLASIYSPEFLSAQSEFVQAEERLKSVAQADSGEYRTTEAL
                     FNSARSRLLLFGAGEKDIAALEENHRPVDRLVIKSPIAGNVVESNLIVGSTVEKGANL
                     FRISDLSFLWVTANVYEKDIRSVERGQKVKVRIASFPGRAFAATVEAVNDVLDETTRT
                     FRVRISVENSTGELKPEMFCECLFSGQSGRPVLAVPATAVQRLGEEQFVFVPKDSLFF
                     EKRAIQTGEELGDWVEVARGLQAGEQVVTEGSFILKSELLKSEVGEEE"
     gene            804..>1706
                     /locus_tag="AC16GM_07400"
     CDS             804..>1706
                     /locus_tag="AC16GM_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2923536.1"
                     /translation="MLARIIEFCLKQKFLVLMGVGFLVFFGAYAFDKLPIDAFPEVTN
                     VQVQVTSQAPGFSPLEVEKLVTYPIEIELSGLPRLQELRSISMSSLSSVWATFEDGVD
                     VYFARQLVLERIISAKERLPQAVTSGLAPVTTAMGEVYRYTLEYPDSIRRPYNQKQLM
                     DLRTVQEWTVKLLLKTVPGVVEVSSMGGFVRQFHVLPRLDRLRKYHLSLKEVFQAIQE
                     NNSVAAGNILNLHSEQNTIRGIGLIRSMPDIENIVVKTHEGTPVFIRDVAEVTLGYET
                     RRGAGVENGEGEAVGGAVMMIKDGN"
BASE COUNT          393 a          420 c          492 g          401 t
ORIGIN      
        1 aagcggggga cagtctggct tccatttaca gcccggagtt tctttccgcc cagagcgagt
       61 tcgtccaggc cgaggaacgg ttgaagtcgg ttgcccaggc cgattcggga gaataccgaa
      121 cgaccgaggc gctttttaat tccgcccgct cccgcctctt gcttttcggt gcaggagaga
      181 aggacatagc tgctctggaa gaaaaccacc gaccggttga ccgcttggtc attaaaagcc
      241 ccattgccgg gaatgtggtg gaaagcaatc tcatagtagg gagcacggtt gagaagggag
      301 ccaatctatt tcgcatttca gatttgagtt tcctctgggt tactgcgaat gtttacgaaa
      361 aagacattcg gtcggtagag cgagggcaaa aagtaaaagt ccggattgcg tcgtttccag
      421 gccgggcttt tgccgcgacg gtcgaagcgg tcaacgacgt tctggatgaa actacccgaa
      481 cgttccgggt tcgaatttcg gtcgaaaact caacggggga actaaagccg gaaatgtttt
      541 gtgaatgcct tttttccggc caaagcggac gtccggttct ggccgtgccg gcgaccgctg
      601 tccagcggtt gggggaggaa cagtttgtct ttgttccaaa ggattcgctt ttttttgaaa
      661 aacgggccat ccaaaccggc gaagaattag gagattgggt tgaagtcgcc cgcggtctgc
      721 aggcaggtga acaagtagta acggaggggt catttatcct taaatccgag ctgctaaaat
      781 ccgaggtggg tgaggaagaa taaatgctgg ccagaatcat cgagttctgc ttaaagcaaa
      841 aattcttggt cctgatgggg gttggctttc ttgttttctt cggcgcatac gccttcgaca
      901 aacttcccat cgatgctttt ccggaagtga ccaacgtgca ggtgcaggtc accagccagg
      961 cccccggttt ttcccccctc gaagtggaaa agctggtcac ctacccgatt gaaatagaac
     1021 tttccggctt accccgcctg caggagcttc gttccatttc aatgtccagt ctttcttcag
     1081 tctgggctac ctttgaggat ggagtggatg tttacttcgc ccgtcagctg gttttggagc
     1141 ggattatttc agctaaagag cggctgccgc aggcggtaac cagtggcttg gctccggtca
     1201 ccaccgccat gggagaagtg taccgctaca cgctcgagta cccagacagc attcgccgac
     1261 cttacaacca aaaacagttg atggacctgc gcaccgttca ggagtggacc gtcaagctgc
     1321 ttttaaagac cgtgcccggg gtagtggaag tcagttcgat gggcggcttt gtgcgccagt
     1381 ttcatgtttt gccccggctc gaccgcttgc ggaaatatca tctttccttg aaggaggttt
     1441 ttcaggcgat ccaagaaaac aactccgtgg ccgccgggaa tatcttaaat ctgcactctg
     1501 aacagaatac tatccgcgga atcggtctca tccgcagtat gccggacata gaaaatattg
     1561 tggtcaaaac tcacgaagga acgccggttt tcatacgcga cgtggccgaa gtcaccctgg
     1621 ggtatgaaac ccgccgggga gcgggcgtgg aaaacggaga gggggaagcc gttggcggcg
     1681 cggttatgat gataaaggat ggcaac
//
LOCUS       DBNGZD010000278         3224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1712422, whole genome shotgun sequence.
ACCESSION   DBNGZD010000278 DBNGZD010000000
VERSION     DBNGZD010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1712422"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="AC16GM_07405"
     CDS             complement(<1..221)
                     /locus_tag="AC16GM_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIR2923537.1"
                     /translation="MQKKKICRWLAAVGIPLLLASGVTGAQKIKVVTTTTDLKALAEA
                     VGKDKVEVTAIARGYEDPHFVEAKPSHML"
     gene            complement(265..1452)
                     /locus_tag="AC16GM_07410"
     CDS             complement(265..1452)
                     /locus_tag="AC16GM_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-regulated TonB-dependent outer membrane
                     receptor"
                     /protein_id="HIR2923538.1"
                     /translation="MRLFGWTFLFVFLAVETGSAQQPAPSPRGGASLNPDISLDGLFS
                     AGYFSKIPNLQFGGHDPKNRGFTAENIELTLGSVVDPYFRADAHIVLLIEDGETVVEV
                     EETYLTTLALPHHLQLLAGQFFTRFGRLNPTHPHTWDWADQPVVNSRFFGPDGVRNPG
                     MQLSYLFPLPFYLELAGSVQNANGETAVSFLSKEGEEIAGRILPKREVHSLGDLLYLG
                     RARASWDLSEAATLVLGSSALLGPNASGPRARTNIFGGDLYFKWRPARASFGWPFFTV
                     QTEALWRNYQAGEFITPVSETLAATTLRDGGFYTQAAYGFTRRWVAGLRFDLARGNDP
                     TDPQRDHRWRGALDLTFYPSEFSKWRLQYDHDVAQHRSDRPIHALFLQWEFLIGAHGA
                     HKF"
     gene            complement(1527..1817)
                     /locus_tag="AC16GM_07415"
     CDS             complement(1527..1817)
                     /locus_tag="AC16GM_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923539.1"
                     /translation="MGRSNLFFRIAFLLWLPLAITFLPLLHHHAGPDGRSAATHCVAC
                     SFSHNLFGGQVTLFALVFSCLVLWFFSLQPALHFSLFSPLGTPTRAPPAFRF"
     gene            complement(1891..2568)
                     /locus_tag="AC16GM_07420"
     CDS             complement(1891..2568)
                     /locus_tag="AC16GM_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6946 family protein"
                     /protein_id="HIR2923540.1"
                     /translation="MSKILIPTKSAEDWKQFLAEPDKHWKRGYSARAIAYCWQEADGL
                     PDDVKKVLMQEPAFEGIEILFAIPEHQVMLNSGTGPSQNDVWVIARTRTSLVSIAVEG
                     KVSESFGPTMAEWSINPSEGKLERLKYLCSVLSLKAKPDDDIRYQFLHRTTSAIIEAN
                     RFQASHAVMLVHSFSLTDEGLTDYKRFLSLFNVNGEADSIIHAGRVSGVALYFAWVRG
                     DKQYLER"
BASE COUNT          753 a          915 c          835 g          721 t
ORIGIN      
        1 ttgagcatgt gcgacggctt ggcctccaca aagtgcgggt cttcgtaccc ccgcgcaatg
       61 gcggtgactt cgactttgtc cttccccacc gcttccgcca gggctttgag atcggtggtc
      121 gtcgtcacga cttttatttt ctgtgcgccg gtgaccccgc tcgctaacag cagaggaatg
      181 ccgaccgccg ccaaccaccg gcaaatcttc tttttttgca tttgtctttt cctttccctc
      241 tgttttgtgt gccgtctacc gtgtttagaa cttatgcgcc ccgtgcgccc cgatcagaaa
      301 ctcccactgc aaaaacagcg cgtgaatggg acgatcactc cggtgctgag cgacgtcatg
      361 gtcgtactgc aagcgccatt tcgagaactc gctcgggtag aaggtgaggt cgagcgcacc
      421 gcgccagcgg tgatcgcgct gcgggtcggt gggatcgttg ccgcgcgcca ggtcgaaccg
      481 aagtcccgcc acccaccgcc gggtgaagcc gtaggccgcc tgtgtgtaga aacccccatc
      541 gcggagcgtc gtcgccgcca gcgtttcgga taccggggtg atgaattcac ctgcctggta
      601 gttgcgccag agtgcttcgg tctgcacggt gaaaaagggc cacccgaaac tggcgcgtgc
      661 cggccgccac ttgaagtaca ggtcgccgcc gaaaatattc gtccgcgccc tcggcccgga
      721 ggcgttcggc cccaaaagtg ccgacgaacc taaaaccaga gtcgccgcct cactcaagtc
      781 ccacgaggcc cgggcgcgtc cgaggtacaa aaggtcgccg agggagtgaa cttcccgctt
      841 gggtaaaatc ctcccggcaa tctcttcccc ctctttactc agaaaactga ctgccgtctc
      901 cccattcgca ttctgaacgc tgcccgccag ttccagatag aacggcagcg ggaaaaggta
      961 cgacaactgc attccgggat tgcgcacccc gtccgggccg aagaagcggc tgttgaccac
     1021 cggctggtcg gcccagtccc aggtgtgcgg gtgggtcggg ttcagacgcc cgaaacgtgt
     1081 gaagaactgc ccggccaaaa gctgcaggtg atgcggtaac gcaagcgttg tcaggtaggt
     1141 ctcttccact tcgacgaccg tttccccgtc ttcgatcaga aggacgatat gggcgtccgc
     1201 ccggaagtag ggatcgacca cgctccccaa ggtcaactcg atattctctg ccgtgaatcc
     1261 ccggtttttg ggatcgtgcc cgccgaactg gaggttcgga atcttcgaga agtagccggc
     1321 gctgaataag ccgtccaggg aaatgtccgg attcaaactc gccccgcccc gcggggaagg
     1381 agccggctgc tgtgctgatc ccgtctctac tgccagaaaa acgaaaagga acgtccaccc
     1441 aaaaagtcgc atcttgccac cgcccttcgc ttgagctaac ccgaccgatt cggcctatgc
     1501 cgaatcagta tcacgaactg atcaggttaa aagcgaaagg caggcggtgc gcgggtggga
     1561 gttccgagtg gagaaaatag ggaaaagtga agtgccggtt gaagcgagaa gaaccagaga
     1621 accaggcagc tgaagactaa agcgaagagg gtaacctgcc cgccgaaaag attgtgtgag
     1681 aaggaacagg cgacacagtg ggtggccgcc gaccggccgt cgggcccggc atggtggtgc
     1741 aggagcggca aaaaggtgat tgccagcggc aaccacaaaa gaaaggcgat tcggaaaaaa
     1801 agattcgatc tccccatctc tggatttatt atacgcaata gagttgaaaa tcgctacgcc
     1861 agccgctgga gcaacttgtt agaccattct ttatctctca agatattgtt tgtctccacg
     1921 aacccacgca aagtataaag caactcctga aacccgccca gcatgaataa tactatccgc
     1981 ttctccgttt acgttgaaca atgaaagaaa acgtttgtag tctgtcaatc cttcatcagt
     2041 caggctgaat gaatgaacaa gcatcactgc atgactcgct tgaaatctgt ttgcttcaat
     2101 gattgctgaa gttgtacgat gtaaaaactg gtatctaatg tcgtcatctg gttttgcttt
     2161 aagactaaga acagagcata aatatttcag tctttccagt ttgccttctg atgggttaat
     2221 actccactca gccattgttg gcccaaatga ctctgaaacc ttgccctcta ctgctatgga
     2281 gacaagactt gttcttgttc ttgctataac ccatacatca ttctgtgatg gtcctgttcc
     2341 actgttcaac atcacttggt gttctggaat ggcaaaaagt atttcgattc cttcaaaggc
     2401 aggctcctgc atcaaaacct ttttcacgtc atcaggtaag ccgtccgcct cttgccaaca
     2461 atatgcaatt gcgcgggcag agtaaccgcg cttccaatgt ttatccggct cagccaaaaa
     2521 ctgcttccaa tcttctgctg acttggtagg gatcaatatc ttgctcataa tctgtcatcc
     2581 tcctttcttt gtaggttctt ttttcttccg ctccaaggcc gcggccaaac ctagcgaaat
     2641 tacctcatga agcagctgaa tgcggccagc caatgatgaa cgatcagcag atttccggcc
     2701 ctctgacaac ccacgaaggc taacaagctc tctaaactgt tcaatgtcct ctcggagcag
     2761 cactgggttg tggagaagct cctcgtggca ctcgtagcag agcaatgctg actcgccctc
     2821 aacaccccag gggcacttct caaagatacc ccctgtctgc gtgccgcgac gattctgcga
     2881 gcgtccaaag aagcgctcag caacgaagtg atgcttcgtt gcgtggctac gcccttcaac
     2941 cgtaggacga gcgtaagtgc cgctccggcg gtgcagagca caaccgcaga tttcgcaatt
     3001 ctcggtcatg gccaatgaca gttaaaaatg attatacagt ctgcataata ccgcaaaggc
     3061 agtcgctttc cttacgcata atacctattc atgtctggag gagtataggt aaaagatacc
     3121 gcaggtgcta cgcctgctcc ggcgggccaa accaatctct gctagtcggg gcaaacactt
     3181 gtcagagatg ccagcgggct taaagtttta tctccttagc aggt
//
LOCUS       DBNGZD010000279         1624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1712599, whole genome shotgun sequence.
ACCESSION   DBNGZD010000279 DBNGZD010000000
VERSION     DBNGZD010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1712599"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..570)
                     /locus_tag="AC16GM_07425"
     CDS             complement(175..570)
                     /locus_tag="AC16GM_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR2923541.1"
                     /translation="MITRLQLRSTKREEALDITSKVELVVKQAGMAEGLCRLYVPHTT
                     ATIFINENEDPAVPKDIVRYLSELVPRGGDYQHREGNADAHIKAALIGATLEIPIQNG
                     KLLLGTWQGIFFCELDGPRQREVIIQLSH"
     gene            complement(567..1487)
                     /gene="ftsY"
                     /locus_tag="AC16GM_07430"
     CDS             complement(567..1487)
                     /gene="ftsY"
                     /locus_tag="AC16GM_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR2923542.1"
                     /translation="MQFSLDRLKSSLAKTKDTFLGKIAAAIGLHPRIDAGLLQDLEDT
                     LIRSDVGVAVTLRLVEQLKKRAQQNNLTGADAVISLLKDEMRHCLVNGSGVATAKTAK
                     EKPHVILVVGVNGTGKTTTIGKLAYHFARQGQKVLLAAADTFRAGAIDQLAIWAGRAG
                     ADLVKSTPGADPAAVAFDAVSAALARRLDLVIIDTAGRLHTKHNLMEELKKIKRAITK
                     VLPNASHEVLLVLDATTGQNGLAQAKLFTQAVGVTGLVLTKLDSTAKGGIIFAIASDL
                     ALPVKYIGIGEQTDDFGLFEPDQFVEALFS"
BASE COUNT          325 a          464 c          471 g          364 t
ORIGIN      
        1 tgggatccaa taccacctac cgggaggtct aaagtttgcg cggcgaggcg ccgcgcctac
       61 ggggaacgag gggtttttta ccacaggaac gaactcaaat caggcggggg tgcaagccac
      121 gatttgaata atctgattga gttgcggtct gcggctgccc ggacgcccta aaactcagtg
      181 gctcaattga atgatcacct cgcgctggcg cgggccgtct aattcgcaaa agaaaattcc
      241 ctgccacgtg cccagaagga gcttgccgtt ctgaatcggt atttcaagcg tagccccgat
      301 caaggcggct ttgatgtgcg cgtcggcatt cccctcgcgg tgctgataat cgccgcctct
      361 gggcacgagt tcggagagat agcgcacgat gtctttgggc accgccgggt cttcgttttc
      421 gttgatgaaa atcgtggcgg tggtgtgcgg gacgtagagc cggcagagcc cctccgccat
      481 cccggcctgc ttcaccacca gctccacctt ggaggtgatg tctaaggctt cctccctttt
      541 ggtggagcgc agttgcagcc gggtaatcat gaaaagagcg cctccacaaa ctgatccggc
      601 tcgaacaggc cgaaatcgtc tgtctgttcg ccaattccga tatatttgac cggcagggca
      661 agatcggaag caatggcgaa aataatccct cctttggccg tcgaatcgag tttggtcagg
      721 accaaaccgg tgacgccaac tgcctgggta aagagttttg cctgggcgag tccgttctga
      781 cccgtcgtgg catccagcac caataaaact tcatgggagg cgtttggcaa gacttttgtt
      841 atggcccgct taattttttt caactcctcc ataaggttgt gtttggtgtg caggcggcca
      901 gccgtgtcga ttatgaccag atccaatcgc cgggctaagg cggcggagac cgcgtcgaaa
      961 gccacggcgg ccgggtcggc tcccggagta cttttcacca gatcagcccc ggcccgcccg
     1021 gcccagatgg ccagctggtc gatggcgccg gcccggaagg tgtcggcggc ggccaaaagc
     1081 accttctgcc cttgccgggc aaagtggtag gcgagtttcc cgatggtggt agtcttgccg
     1141 gtaccgttca cccccactac caggatgacg tgcggcttct cttttgcggt tttagcggtg
     1201 gcaacgcctg aaccattcac caggcagtgc cgcatttcgt ctttcaaaag actgatcacg
     1261 gcgtcggcac cggtcaggtt attctgttgg gcccgctttt tcaactgctc gactaaacgc
     1321 aaggtgaccg ccacgccgac gtcggagcgg atgagggtgt cttccaagtc ctggagcaag
     1381 ccagcgtcaa tcctcggatg gagcccgatg gcagcggcga ttttgcctaa gaaggtatct
     1441 ttggtttttg cgagactcga tttcaatcgg tcgagtgaga attgcatcag ttttccctat
     1501 cacctatgac caattacctg gttcaccttt gacggccacg aaggcctatg ctaccaaagt
     1561 tgcaacgagc tgcccgatgt ccacaatcgc tttccgagtg ggcgaagcgc cccccctgcg
     1621 cgac
//
LOCUS       DBNGZD010000280         8606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_602254, whole genome shotgun sequence.
ACCESSION   DBNGZD010000280 DBNGZD010000000
VERSION     DBNGZD010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_602254"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /gene="purL"
                     /locus_tag="AC16GM_07435"
     CDS             <1..1682
                     /gene="purL"
                     /locus_tag="AC16GM_07435"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR2923543.1"
                     /translation="GRMVRAVAKGEGNPVVIVGAATGRDGIHGATFASEEISEASEAR
                     RPSVQVGDPFMEKLLLEATLEIIKEDLVVGIQDMGAAGLTCSTSEMAARGGCGMEVEL
                     AHVPLRETGMTPYEIMLSESQERMLVVVKSGSEKRVSEIFTKWGLNSAIIGKVTADGL
                     LRVKVHGRVVAEIPANSLVLGGGAPVYVREKKKPEYIDQLQQIDFSTVPAPADFNNVL
                     LKLLASPNIASKEWVTRQYDTSVRTNTVVKPGSDAAVLRLRKTNKALAVVTDCNARFC
                     YLNPRRGAMIAVAEAARNVVCAGARPLAITNCLNFGNPYDPEVFWTFAEVIAGMGAAC
                     RAFETPVTGGNVSFYNEDPERAIYPTPVIGMVGLVEDVKHITTQWFKDEGDVILLLGE
                     NREELGGSEYLKTIHGTIGGEVPALDLDFEKRVQAVALESIQSGWVKSAHDCSDGGLA
                     VALAECCITNPQKTVGAEVDLEDKIRPDALLFGETQSRIILSASSQDASRILELAKTQ
                     KVPCRVIGRVVGSRLRMDRRLDSTRKWISEDCGRLAEAFQTSLGQVLERFD"
     gene            1808..2206
                     /locus_tag="AC16GM_07440"
     CDS             1808..2206
                     /locus_tag="AC16GM_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR2923544.1"
                     /translation="MRKQKSLRSLAVLGSAVFFVVLTTWAISCSKKKSTNPGGGAIVF
                     QHNLNVTIRDNSFSPQYDSISVGQTITWTNAGSVIHNVTSAGGFTSSPPDLSPGASYP
                     STGGLVFNTPGDYPYQCTHHAGMTGTIRVK"
     gene            2320..2703
                     /locus_tag="AC16GM_07445"
     CDS             2320..2703
                     /locus_tag="AC16GM_07445"
                     /inference="COORDINATES: protein motif:HMM:NF021486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HIR2923545.1"
                     /translation="MAVFQKHFTLEEARGLLPQLKKIFGEIKAIRAGLETAEGDINQF
                     IEKTSGNGGSGHSPAFLQASTRIRSLLSEVAALGVQIKDINRGLVDFPVIIDGEEVLL
                     CWEYGEEDIGFWHDLESGYAGRQPL"
     gene            2799..2951
                     /locus_tag="AC16GM_07450"
     CDS             2799..2951
                     /locus_tag="AC16GM_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923546.1"
                     /translation="MELPQTLKFLNLGFWVIHIFAITIIFTLGYAMGKKKARRLSTDS
                     QRIKTD"
     gene            3026..4726
                     /gene="hutU"
                     /locus_tag="AC16GM_07455"
                     /pseudo
     CDS             3026..4726
                     /gene="hutU"
                     /locus_tag="AC16GM_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362703.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
     gene            4754..6004
                     /gene="hutI"
                     /locus_tag="AC16GM_07460"
     CDS             4754..6004
                     /gene="hutI"
                     /locus_tag="AC16GM_07460"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR2923547.1"
                     /translation="MAAELVITDIGQLLTLYSPVPAPRRGKAMADLGLIRNAAVAVTN
                     GRIAAVGTKQTLLKKISRKTKVISAGGRLVTPGLVDPHTHAVFAGNRAGEFEQRLAGQ
                     TYQEIAAAGGGIQATVRATRRASERELETRLLYHLTGMLSWGTTTVEVKSGYGLSLTS
                     ELKLLNVIKRAQKKTPLDLVPTFLGAHTVPREFRHNRKEYVKLLCERMLPAITRHKVA
                     CFSDVFTERGAFSLAESKTIQRTARSLGFALKFHVDELSPLGGAELAAEMGATSADHL
                     EFVSERGIRALARSGTVAVLLPSVSLFLGLKRWAPARRLIAAGVPVALASDFNPGTSP
                     TYSLPLVMSLACFELKMTPAEVWAAATLNAAYAAGLGRRVGSLKPGKQADLVIWQAAD
                     YREIPYHLGVNQVERVIKEGKVVL"
     gene            6001..6489
                     /gene="lptE"
                     /locus_tag="AC16GM_07465"
     CDS             6001..6489
                     /gene="lptE"
                     /locus_tag="AC16GM_07465"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HIR2923548.1"
                     /translation="MKFVPLAVISFGLFLAGCGPYSFSSSGLGSYRTIAIPLFDNQTA
                     EFGLREALTAGITQGFVQDNNLKVTGEAGADLVLSGSITRYERVPYTFDRAEQVKEYI
                     VRIWAAVTCTGTKEQKTVWEEKNLLGFGIYSATSESEADGRTRAITKLGQDIVNRTVR
                     TW"
     gene            6566..7510
                     /locus_tag="AC16GM_07470"
     CDS             6566..7510
                     /locus_tag="AC16GM_07470"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2923549.1"
                     /translation="MKKVFINSLVAGAALLCMSGDTQATNSKVGTTAYSFLKIGVGAR
                     AVALGGAFTGLADDESALYYNPAGLVSTGKRAVTTSYVNYLSDIQSGFVGYIHPYTET
                     QRFGVAVNYFNYGSFTGLDQTGAPTGDFTPSDLALAVSYAAGFKGGLQVGLSSRFVFE
                     KIDSLSSTGLMFDLGALYKFKDGRTQAGVALQNAGFQLSGLTSMHKDDLPVVVRVGFA
                     HFLRGLPLVFAADVAKPVDNDFHIDVGAEFLQLKPVYLRLGWSSIGSAIRTSSDNNKL
                     AGFAGGFGVIYQKYEIDYAFVPYADLGSAHRVTVAARF"
     gene            7564..8013
                     /locus_tag="AC16GM_07475"
     CDS             7564..8013
                     /locus_tag="AC16GM_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923550.1"
                     /translation="MRRQTERVANAIFGLLFLCFLSGCSRKPQPKETLYRFFAGVVNR
                     DSTAIQDCLDLERTATEAQLQAIGVDSIPIVPEARQKFLIHELAAGRVNQRWREHRIV
                     VGETALRGDTAMVEVSFFHRPTGRQYYNKMGLIFSGGRWKIVSFKLL"
     gene            8120..>8606
                     /locus_tag="AC16GM_07480"
     CDS             8120..>8606
                     /locus_tag="AC16GM_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR2923551.1"
                     /translation="MLAKVFSSAVHGIDAYLVEVEADITATLPAFATVGLPDGAVKEA
                     KERVMSAIKNSDFIFPNKKVTINLAPADVKKEGSAFDLPMAVGILSATGQISRDDLTP
                     YVFLGELSLDGGLKPVDGVLPMALSLKTDGKKGLVVPRENAKEAAMAAGVPVFPVESL
                     SQ"
BASE COUNT         1873 a         2246 c         2717 g         1770 t
ORIGIN      
        1 aagggcggat ggtgcgggca gtggccaaag gggaagggaa tccggtcgtc atcgtcggcg
       61 cggcgacggg ccgggacggg attcacggag cgacctttgc ctcggaggaa atttcggaag
      121 catcggaagc caggcggccg tcggtgcagg tgggcgatcc cttcatggaa aaattgctct
      181 tggaagcgac tttggaaatc atcaaggaag atttagtcgt cggcatccag gatatggggg
      241 cggctgggct gacctgttct acttccgaaa tggcggcgcg cggcggctgc ggaatggagg
      301 ttgaattggc tcacgtcccg ctgcgagaga cggggatgac cccctatgaa atcatgctct
      361 ccgagtcgca ggagcggatg ttagtcgtgg tcaagtccgg cagcgagaag cgcgtcagcg
      421 agattttcac caaatggggg ctcaattcgg ccatcatcgg gaaggtgacc gccgacggcc
      481 tgctgcgggt taaagtccac gggagggtcg tggctgagat tccagcgaat tcgctcgttt
      541 taggcggtgg agcgccggtc tatgtccggg aaaagaaaaa acccgaatat atcgaccaac
      601 tgcaacaaat tgatttttca accgttcctg cacctgctga tttcaataat gtcctcctga
      661 aacttctggc atcgcccaac atcgcctcga aggaatgggt aacccggcag tacgatacct
      721 cggtgcggac caatacggtc gttaagcccg ggtcggatgc ggcggtgctg cgcctgcgga
      781 agacgaacaa ggcgctggcg gtcgtcaccg actgcaacgc ccgtttctgc tatctcaacc
      841 cccggcgcgg ggcaatgatt gcggtggcgg aggcggccag aaacgtcgtc tgtgccgggg
      901 cgcggcctct ggccatcacc aactgtttga acttcggcaa cccgtatgat ccggaagttt
      961 tttggacgtt tgccgaagtg attgccggga tgggggcggc ctgccgggcg ttcgagacgc
     1021 cggtgacggg cgggaacgtc agtttctaca acgaagaccc tgagcgggcg atttacccca
     1081 cgccggtgat cgggatggtg ggtttggtcg aggatgtgaa acatatcacc acgcagtggt
     1141 ttaaggacga aggcgacgtc attctccttt tgggcgaaaa ccgggaagaa ttgggcggct
     1201 cggaatacct gaagacgatt cacggtacca ttgggggcga agtccccgcg cttgaccttg
     1261 attttgaaaa gcgggtgcag gcggtggctt tggagtcaat tcaatccggc tgggtgaagt
     1321 cggcgcacga ctgctccgac ggcggcttag cggtggccct ggcggagtgc tgtattacga
     1381 acccgcagaa gacagtgggg gcggaggttg accttgagga taaaatccgt cctgatgcgc
     1441 ttctgtttgg agagacacag tcacggatta tcctcagcgc ttcgtctcag gatgcctctc
     1501 gaatccttga acttgctaag acccaaaagg ttccctgccg ggttatcggc cgagtggtcg
     1561 gaagcagatt gcggatggat cggagactcg actctacgag aaagtggata agcgaggact
     1621 gcgggcggct ggccgaagcc ttccagacct ctctggggca ggtactggag cggttcgact
     1681 agctcaccac aagcggttaa agtagtcggg aacaaaatca accgcatact ggttactact
     1741 atttagtact atcggaacgc gcaggaaagc gtattctgtt caaaaggaga agaaaggaga
     1801 agaaggtatg cggaaacaaa aatctctcag aagtctggct gtcctcggta gtgcggtgtt
     1861 cttcgtcgtt cttaccacct gggcgatttc gtgcagcaaa aagaagagca cgaacccggg
     1921 tggtggggcg attgtgtttc aacacaatct gaatgtcacc ataagagaca attccttcag
     1981 tccccaatat gacagcattt ccgtaggtca aacgataacc tggacaaacg ccggatcggt
     2041 catccataat gtaacctcgg ctggtggatt cacttcctcc ccccccgatc tttcaccagg
     2101 cgcatcgtat cccagtacag gtggtcttgt atttaatacg cccggggact acccatacca
     2161 gtgtacccac cacgctggta tgaccggtac gatacgggta aagtgataga ttcgagacgg
     2221 gctctaaaaa aatcaggcac aatgtcagtt tgaccccgcc cagcggggtc tttttttgcc
     2281 cttgacaccg ggcggggatt gcgttattag cgggtcggga tggcggtgtt tcaaaagcat
     2341 tttacgctgg aagaagcccg gggcctcctg ccgcaactga agaaaatctt cggcgagatc
     2401 aaggcgatcc gcgccgggct ggagacggcg gagggggata tcaatcaatt catcgaaaaa
     2461 acctccggca acggcggcag cggccattcg ccggcttttt tgcaagcaag tacgcggatt
     2521 cgctcacttt tgagcgaagt ggcagcgctg ggggtgcaga tcaaagacat caaccggggg
     2581 ctcgtcgatt tccccgtgat aatcgatgga gaagaagtcc tcctctgctg ggagtacggg
     2641 gaggaggata tcgggttctg gcatgattta gagagcgggt atgcgggaag gcaaccccta
     2701 tgaaagagaa aaatagtagc cagtagccag gggccagtag ccagaaacct aatttctaaa
     2761 tcctatatcc gaactttggg ctacgtgagg gaggagagat ggaactaccg cagacgctga
     2821 aatttctcaa tctggggttc tgggtcattc acatttttgc cattaccatc atctttaccc
     2881 tcgggtatgc aatggggaag aagaaagcgc ggcgactgtc aacggattcg caacggatta
     2941 aaacggatta aaggagtgga ttcttctccg taaaacgttg aggcgcgttg acggccagcg
     3001 aatatccccg tagtttacaa ttgcaatgcc gcttgtagcc gaaaggaaaa taaaagaaaa
     3061 gtcggacttt gcgtataagc ccatccgggc ggcgcgggga aagaaaattg cctgcaaggg
     3121 gtggcagcag gaagcggccc tgcggatgct ccagaacaat ctcgacgagg aagtcgggga
     3181 aaatccgcgg gagttggtcg tctacggcgg gcggggaaag gcggcgcgga actgggagtg
     3241 ttatttcgcc atcgtccggg cgctcaagaa cctcaaaaat gacgaaacgc ttctcgttca
     3301 atcaggcaag ccggtgggca ttgcgagaac gttcgaacac gcgccgcggg ttctgatcgc
     3361 caattcgctt ttggtgccga agtgggcaac cgccgaggtg ttccgcgacc tggaagcgct
     3421 ggggctcatc atgttcggcc agatgaccgc ggggtcgtgg atttacatcg ggacgcaggg
     3481 gattctgcag gggacgtacg aaacctttgc ggcggtggcc aataagcact tcgggggcag
     3541 tctggcggga cgcttggtcc tgaccggcgg gatgggcggc atggggggcg cgcagccgtt
     3601 atccgcgacg atgaacggcg cggcaatgct ctgcgtggaa gtcgatccga agcgaatcaa
     3661 aaagcgtctg gccaccggtt actgcgatgc gatcagtcac gatctcgacg atgcgctcaa
     3721 taaagttctg gctgctaaaa aagagcggcg gccgctctcc gtaggattgg tgggcaactg
     3781 cgcagaggtc ctgcccgaaa ttttgcggcg cgggattatc cccgacgtgg tcaccgacca
     3841 gacttcggcc cacgatgaac tgaacggcta cgttcccgcc ggtttgcagt tggaagaggc
     3901 gctgcgcctg cggcgcgagc agccggagga gtacatcaag cgggcgtaca tttcgatggc
     3961 cgagcactgc cgggcgatgt tgggattcca gaaagcgggc gcggtggtct ttgactacgg
     4021 caacaacctg cgcgggcagg ccgagaaggc cggggtcgaa aatgccttcg attaccccgg
     4081 ctttgtgccg gcgtacatcc ggcctttatt ctgcgaggga cgggggccgt tccgctgggt
     4141 ggcgctctcc ggcaaaccgg aagaaattca aacgaccgat gcggtcgtcc tgaaggaatt
     4201 cgccaaaaac gaagcgctcg tgcgctggat ccgtctggca caaaaaaaag taaaatttca
     4261 gggactgccg gcgcggattt gctggctggg gtacggggag cgggcgcgct ttggcgcggt
     4321 catcaataac ctcgtcaaaa agaaaaaatt aagcgcgccg gtggtgatct gacgcgatca
     4381 cttggatacc ggctcggtgg cctcgcccta ccgcgagacc gaggggatga aagacggctc
     4441 ggacgccatt gccgactggc cgattttgaa cggccttttg aatgcggtct ccggcgcgtc
     4501 gtgggtttcg gtccatcacg ggggcggggt ggggatcggg ctttcgatcc acgccgggca
     4561 ggtggtggtg gccgacggca cgccgcaaat ggcgaaacgc atcgagcgcg tgctgaccaa
     4621 cgatccgctc tcgggcattt tccgccacgt cgatgcaggg tacgaagaag cgaagcaagt
     4681 agcccgtaag ttccgcatca aaattccgat ggaacgccgc cagtgaggag gagacggtgg
     4741 cgggtgcgtc gagatggcgg ctgagctcgt catcaccgac atcggtcagc ttctgacgct
     4801 ttattcgccc gttccggcgc cgcgcagggg gaaggcgatg gcggatctcg gcctgattcg
     4861 caatgcggca gtggccgtca ctaacgggcg aattgcagcg gtcgggacga aacagacact
     4921 ccttaaaaaa atctcaagaa aaaccaaagt tatatcagca ggggggcggc tggtaacgcc
     4981 cggtttggtt gacccgcaca cgcacgcggt gtttgccgga aaccgcgccg gtgaatttga
     5041 acagcggctg gcgggccaga cctaccaaga aattgccgca gcaggcgggg gcattcaagc
     5101 gacagtgcgc gccacgagga gagcttccga gcgagaactg gaaacacggc ttttatatca
     5161 tttgaccgga atgctctctt ggggcacgac gaccgtggaa gtgaaaagcg ggtacgggct
     5221 gtcgctaaca tcggaactga aattgcttaa cgtaatcaag cgagcgcaga agaaaacacc
     5281 tcttgatctt gttccgactt ttttgggcgc gcatacggtt ccgagagagt ttagacacaa
     5341 ccgaaaagag tatgtcaaac tcctctgtga acggatgctg ccggccatca ccagacacaa
     5401 ggtggcgtgc ttttcggatg ttttcactga acggggcgcc ttcagtctgg ctgagtcgaa
     5461 aacaattcag cgcacggccc ggagtttagg ttttgcactg aagtttcacg ttgatgaact
     5521 ttctccactc ggcggggcgg aactggcggc ggaaatgggg gccacgtcgg cggatcactt
     5581 ggagtttgtt tccgagcgcg gcatccgggc gctggcgaga agcggaaccg tagcggtgct
     5641 cttgccttcg gtttcgcttt ttctgggctt aaaacggtgg gcgccggcgc ggcggttgat
     5701 tgcggccggc gtgccggtgg ctctggcttc cgatttcaat cccggcacct cccccaccta
     5761 ctctctgccg ctcgtaatga gtctggcctg tttcgaattg aaaatgaccc cggcggaagt
     5821 ctgggccgcc gcaacgctca atgccgcgta cgccgccggt ctcggtcggc gggtcggcag
     5881 tctcaaaccc gggaagcagg cggacctggt catctggcag gcagcggatt accgggagat
     5941 tccgtatcat ctcggtgtga accaagtcga gagggtcatc aaagagggga aagtcgttct
     6001 gtgaaatttg tgccgttagc cgtcattagt ttcggtctgt tcctggcggg ctgcgggccg
     6061 tattcgtttt ccagttccgg gttagggagt taccggacaa ttgcgattcc gctcttcgac
     6121 aaccagaccg cggaattcgg cctgcgggaa gcgctgaccg ccgggatcac ccagggattc
     6181 gtgcaggaca ataacctcaa agtaaccggt gaagccgggg ccgatttagt gctctccggc
     6241 agcattaccc gttacgagcg ggtgccttac acctttgacc gggccgaaca ggtgaaggag
     6301 tacatcgtgc ggatctgggc ggcggttacc tgcaccggga cgaaagagca gaagacggtc
     6361 tgggaggaga agaatctttt agggttcgga atctacagcg ctacctcgga atccgaagcg
     6421 gacggccgca cgcgggcaat cacgaaattg gggcaggata tagtcaaccg gacggtgcgg
     6481 acatggtgag attttcattg caaattgaaa aaaaactgtt acatttatga gaaagatgtt
     6541 gaacaaatca gccgtggagc attccatgaa aaaagtattc ataaacagtc tcgtggctgg
     6601 tgcggccttg ctctgtatgt caggcgacac gcaagcaacc aactcgaagg tgggaacgac
     6661 cgcttactcg tttttgaaaa tcggcgtggg ggcgcgggcg gtggctttgg gcggcgcctt
     6721 caccggactg gcggatgacg aatcggcact ctactacaat cccgccggac tggtttccac
     6781 cggtaaacgg gcggtgacga ccagttacgt caattacctt tccgacatcc aatcgggctt
     6841 cgtcggctac attcacccct ataccgaaac gcagcgcttc ggggtggcgg tcaactactt
     6901 caactacggt tccttcaccg ggctggatca aaccggcgca ccgaccggcg atttcacccc
     6961 cagcgatctc gcgctggcgg tctcctacgc cgccggcttc aaaggcgggt tgcaggtggg
     7021 tctctccagc cgtttcgttt tcgaaaaaat cgacagtctc agttccaccg gactgatgtt
     7081 cgacctggga gcgctctaca aattcaaaga cggtcgcacg caggcgggcg tggcgctgca
     7141 gaacgcgggg ttccagttgt cggggctgac ttccatgcat aaggacgacc tgccggtggt
     7201 ggtccgggtc gggttcgctc actttttgcg cggactgccg ctcgtttttg ccgccgatgt
     7261 ggccaagccg gtggataatg atttccatat tgacgtcggt gccgaattcc tgcagctcaa
     7321 accggtctat ttgcgtctgg ggtggagcag tatcggcagc gccatccgca ccagttcgga
     7381 caacaacaaa ttagcggggt ttgccggagg tttcggcgtg atttatcaga aatacgagat
     7441 tgattacgcc ttcgtgccgt acgccgatct cgggtcggcc caccgggtga cggtggcggc
     7501 gcggttctaa cgtcagccga gcgagctgat cccggttcgt tctccttccc gagcaattcg
     7561 ccgatgaggc gccagaccga acgagtcgct aatgcaatct tcggattact ctttctttgt
     7621 tttctctccg gttgttcgcg gaagccgcaa ccgaaggaga cgctgtacag gttttttgcc
     7681 ggtgtggtca atcgggacag taccgccatc caagactgcc tcgatttgga acggacggcg
     7741 acggaagcgc aactgcaggc cattggcgtg gactcgatcc cgatagtacc ggaagcacgc
     7801 cagaagttcc tcatccacga attggccgcc ggacgggtga accagcgctg gcgggagcac
     7861 cggattgtcg tcggagaaac agcattgcgc ggagatacgg cgatggtcga agtttccttc
     7921 ttccatcggc cgacggggag gcagtactac aataagatgg ggttaatttt ttcaggcggg
     7981 cgctggaaga tagtgtcatt taagctcctc taacatcgta ccacaccccg gctttcctcg
     8041 gaaaaagata tgctgagagc ttgagcgacc ccgaaggcgg tttgacaaac gggttccggg
     8101 gggtcatttt ttctctgcca tgctcgccaa agtcttctct tcggcagtcc acgggatcga
     8161 tgcgtactta gtcgaagtgg aagcggacat caccgccacg ctgccggcct ttgccacggt
     8221 cggcctgccc gacggggcgg tcaaggaagc caaggagcgg gtgatgtcgg cgatcaaaaa
     8281 ctccgatttc atcttcccca ataagaaagt caccatcaac ttagccccgg cggatgtgaa
     8341 gaaagagggg tcggcgttcg atctgcccat ggccgtaggc attctctccg ccactggaca
     8401 aatttcgcgc gacgacctga cgccttatgt cttcctcggc gaactctcgc tggacggcgg
     8461 cctcaagccc gtggacggcg ttctgccgat ggcgctttcg ctgaaaaccg acgggaagaa
     8521 agggctggtc gttccccggg agaatgccaa agaggcggcg atggctgcgg gcgtgccggt
     8581 tttcccggtg gagtctttga gccagg
//
LOCUS       DBNGZD010000281         8532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1574149, whole genome shotgun sequence.
ACCESSION   DBNGZD010000281 DBNGZD010000000
VERSION     DBNGZD010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1574149"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="gcvPB"
                     /locus_tag="AC16GM_07485"
     CDS             complement(<1..964)
                     /gene="gcvPB"
                     /locus_tag="AC16GM_07485"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR2923552.1"
                     /translation="MIGENEKKIVEKTIFELSSPGRIGHRLPEADVPVHPVEELLPAS
                     QRRQKDAALPEVSEIDVVRHFVRISTLNHHIDKGFYPLGSCTMKYNPKIHEETARYPG
                     FAGLHPLQPEETIQGALRLMYDLASYLAEIGGMEQVTLQPAAGAQGELTGLMMIRAYH
                     ESRGKSRKKVIIPDSAHGTNPSSVVTSGYELVTVKSCDDGLVHIEDLQRLLDDEVAAF
                     MVTNPNTLGFFEREILKISELVHGVGAQMYMDGANLNALLGIVRPGDMGFDVVHFNLH
                     KTFSTPHGGGGPGAGALGVKSHLVPFLPVPIVSKKEKGGKEVFFF"
     gene            complement(978..1646)
                     /locus_tag="AC16GM_07490"
     CDS             complement(978..1646)
                     /locus_tag="AC16GM_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923553.1"
                     /translation="MEESTYVDPKVIRLQDQLVFAKVNGRADTVLARQYGISGYPTII
                     LLTSTGEEIDRLWGYFPPNDFEKKVTDYLAGKGTLPALEKQLAKEPENIDLLMQVGEK
                     YASRSQFEKSIGLYEQVIAKDPENKSGKIPTALSGIGDVLYRGKKYMEAKERYQTIVE
                     KYPTAEEYNDAVTSIPLMHELAGDTALAISLYQQVLKEHPNHPDTAWVQKRLAKLTAP
                     PATK"
     gene            complement(1662..1826)
                     /locus_tag="AC16GM_07495"
     CDS             complement(1662..1826)
                     /locus_tag="AC16GM_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923554.1"
                     /translation="MRRSLLFAAGVFCGSSLALAADKPKEITWQDYTQATTAAKKTHQ
                     PVLIDFWRPG"
     gene            complement(1874..3277)
                     /gene="gcvPA"
                     /locus_tag="AC16GM_07500"
     CDS             complement(1874..3277)
                     /gene="gcvPA"
                     /locus_tag="AC16GM_07500"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR2923555.1"
                     /translation="MTYFPHTPNDRRKMLERIGVGRFEDLLRELPPELVLQKPLALPK
                     RLSELETAAHLRSLAGENGSSQEYVSFLGGGIYDHYVPAAVDAILRRPEFYTAYTPSQ
                     AEVAQGTLQAIYEFQSLICQLTGMEVSNASMYDGPSAVAEAALLATGQTGRKEILFPA
                     TLNPHYQKVLATYSRGGAMKLAMVRGAEGTIDLEDLKKKITDRTAAVIVQQPNFYGCL
                     EKPAEFEPLVHQAGALLIAVCDPISLGLLAPPGEYNSDLAVGEGQSLGIPTQLGGPGF
                     GFFAGKKELVRRMPGRIAAETVDAKGRRGYVLTLQTREQHIRREKATSNICTNQALCA
                     LAAAVYLSLLGRAGIKDIAELCLQKAHYAVDQLARLPGFRLRFASPFFKEFVLETPVP
                     ARKIIRAALKKGIFAGVDLGQYQRELKNCLLIAVTEKRTRAEIERLVDLLAKVTQARQ
                     TSIPGPERQSAPQKVTV"
     gene            complement(3495..3875)
                     /gene="gcvH"
                     /locus_tag="AC16GM_07505"
     CDS             complement(3495..3875)
                     /gene="gcvH"
                     /locus_tag="AC16GM_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR2923556.1"
                     /translation="MLIKPELKYTKEHEWVKVEGAIATVGITDYAQGELGDVVFVELP
                     KVGTAVEQMKPFGTIEAVKAVSEMYAPLSGEVTEVNVRIETEAGIINKDPYGEGWLIK
                     IKVKNSQELAALLDASRYEALIKT"
     gene            complement(4008..5423)
                     /gene="accC"
                     /locus_tag="AC16GM_07510"
     CDS             complement(4008..5423)
                     /gene="accC"
                     /locus_tag="AC16GM_07510"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR2923557.1"
                     /translation="MFKKVLIANRGEIALRVIRACNELGIATVAVHSEADANSLHVRF
                     ADEDVCIGPGLAKDSYLDPKRIISVAEVTNADAIHPGYGFLAENADFAEICQACNLVF
                     IGPTPQQIRLMGDKLAAKNTMAKAGVRVIPGSDGPVNTLEEALEVAEEIDYPLMIKAA
                     AGGGGRGLRCAMDPSDLKSMFDTARAEAQAAFGNPAVYLEKFLPNPRHVEIQILGDHH
                     GNVIHFGERDCTIQRRHQKLLEESPCPILPERRRHQMAEAAVRGAKNIKYRGAGTVEF
                     LYDPTGHFYFMEMNTRIQVEHPVTEEVADIDLVKEQIKVAAGEKLSLRQGDVKLSGHA
                     LECRINAEDPERDFMPCPGELSAFHIPGGHGIRVDTHAYVKYVIPPFYDSLVAKVIAH
                     GKTREEAIIRMRRALEEFTIEGIKTTIPFHLKVLHHERFLAGQYDTAFVEELQRELGV
                     ERVTAVSEPAAPVPEGVQASA"
     gene            complement(5646..6767)
                     /locus_tag="AC16GM_07515"
     CDS             complement(5646..6767)
                     /locus_tag="AC16GM_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR2923558.1"
                     /translation="MNLKQMLAEMQSQRASDLHLRVGLRPTLRVDGCLTPLEAEPLTN
                     EGLEAILNQILTPPQKERFLKRNEMDLALSVVNMGRFRINIYRQRGTVGVAIRAVSTE
                     IPSFEELNLPEIVCKLAENRRGMIVVTGTTGSGKSTTLAAMIEYINATRAENILTIED
                     PIEYVYSDKQSIISQREVGGDTESFPSSLRHAFRQDPDVILIGEIRDLDTMSIALTAA
                     DTGHLVLTTLHTLNAVETISRIISFFPPHQHQQIRLLLASTLKAVICQRLLPRTDIPG
                     RVPATEVLINTLSVREAIIDPMKATTIIELIESGSVQYGMQSFDQSIMKLYRQGAISY
                     EEAMAQTSNPDDFDLRVKGITGAADRGWTEFEQSQKPRV"
     gene            complement(6843..7364)
                     /gene="accB"
                     /locus_tag="AC16GM_07520"
     CDS             complement(6843..7364)
                     /gene="accB"
                     /locus_tag="AC16GM_07520"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR2923559.1"
                     /translation="MREQTLRKLIRLVEQSAIESLEIWSWGRRVKITKRLSAENGQPA
                     SGGAPALPHLHVLAPTQRPSHFTPLTAAQSGNNPAVAGVEVPPEIPTDRVAAIKSPMV
                     GTFYRAPAPDAKSYVELGQVIEKGQVVCIIEAMKLMNEIESDVAGRVVKILVENARPV
                     EFGQPLFFVEPTG"
     gene            complement(7442..>8532)
                     /locus_tag="AC16GM_07525"
     CDS             complement(7442..>8532)
                     /locus_tag="AC16GM_07525"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR2923560.1"
                     /translation="LRHLDTLLRRRMKVLAAVARERLDCLVVTSGANVRYLSGFTGSN
                     GLVVLTPRRTYFFSDHRYDLQAHQEVPGAKIVIGTGDLFALLPQIPFLRTPKVKMGCE
                     SGHVTVRGWELLKKHLPQALPLPTENLVERVAAIKDAGEIAAIEQAAGIADRCFEKIV
                     NFLRPGLAEREVAAELEYQMKQDGAEGAAFETIVASGPRSALPHGRASDRKIQKGDFV
                     TLDFGAVVNGYASDLTRTVVMGRASSRQKKIYRIVLEAQKRVINAARAGIVAKDLDAR
                     ARGYIAHWRLGKYFQHGLGHGLGLEVHQLPGINSRSTEKLLAGMVVTIEPGVYLPRWG
                     GVRIEDDVLITHGGCRVLTQADRTLLEL"
BASE COUNT         1611 a         2550 c         2469 g         1902 t
ORIGIN      
        1 cgaagaagaa aacttccttt cctccttttt cttttttact cacaatcgga acgggcaaga
       61 acggcaccag atgagatttg acgccgagcg caccggcacc cggtccgccc ccaccgtgag
      121 gcgtcgaaaa agtcttgtgc aggttgaagt gcactacgtc aaagcccata tcaccgggac
      181 gaacgatacc cagaagtgcg ttgaggttgg caccatccat gtacatctgg gcaccgacgc
      241 cgtggactaa ttcgctgatt ttcaaaatct cccgctcgaa gaaccccagc gtattgggat
      301 tggtcaccat aaaggcggcg acctcgtcgt ccaaaagccg ctggagatcc tctatgtgca
      361 ccaaaccgtc atcgcacgat tttaccgtca ctaactcata cccggaggtg acaaccgatg
      421 aaggattggt tccgtgagcg gagtcgggga tgatgacttt tttgcgcgac ttgccccgcg
      481 actcatggta cgcgcggatc atcattaaac cggtcaactc cccctgcgcg ccggcggcgg
      541 gctggagcgt gacctgctcc ataccgccga tttcagcgag gtaggacgcc aggtcataca
      601 tcaggcggag tgcgccctga atggtttcct ccggctggag cggatgaagt cccgcgaaac
      661 ccggatagcg cgcggtctcc tcgtggattt tgggattgta cttcatcgtg caggagccga
      721 gcgggtagaa gcccttgtcg atgtggtggt tcagggtcga aatccgcacg aagtggcgca
      781 ccacgtcgat ttcggaaacc tccggcagtg cggcgtcctt ttggcgtctc tgcgacgcag
      841 gcaaaagctc ctcgaccgga tggaccggga catcggcctc aggcagccga tgtccgatcc
      901 gtccggggga actcaactcg aagattgtct tctcgacgat ctttttttcg ttctcaccta
      961 tcacttatca ctcataacta ttttgtcgcc ggcggagcgg tgagtttggc cagtcgtttc
     1021 tggacccagg ccgtgtccgg gtggttggga tgttctttca atacctgctg gtaaagcgag
     1081 atcgccagcg ccgtgtcgcc ggccaactca tgcatcaacg gtatcgacgt caccgcgtcg
     1141 ttgtattcct cggccgtcgg atacttttcc acaatcgttt ggtaccgctc ttttgcctcc
     1201 atgtacttct tgccccgata caggacgtcg ccgatgccgc tcaaggcggt cgggattttg
     1261 ccgctcttat tctccggatc tttggcgatc acctgctcgt acaagccgat cgatttctca
     1321 aactggctcc tgctcgcgta cttctccccc acctgcatca gaagatcgat attctccggc
     1381 tccttggcca actgcttttc taaagcgggg agcgttcctt tgcccgccag gtagtcggta
     1441 actttctttt cgaaatcgtt cggcgggaag tagccccaca ggcggtcaat ctcttcaccc
     1501 gtgctggtga gcaggatgat cgtggggtag ccggagattc cgtactgacg tgccagcacc
     1561 gtgtcggctc tgccgttcac cttcgcgaag accaactggt cttggaggcg gatgactttg
     1621 ggatcgacgt aggtcgattc ctccatccgc gcgcaccact ttcaccccgg ccgccagaag
     1681 tcgattaaaa ccggctggtg tgttttcttc gccgctgtcg tggcctgcgt gtaatcctgc
     1741 cacgtaatct ctttgggttt gtcggcggca agcgccagtg acgacccgca gaacactccc
     1801 gctgcgaaga gcagacttcg cctcatcact cctcctttcg ttctgtttgg tcattctcat
     1861 cgtccgctcg ttctcataca gtcacttttt gcggtgcgga ctgccgttcg ggaccgggaa
     1921 ttgaggtctg cctggcttgc gtaacttttg ccaggaggtc aaccaggcgc tcaatctccg
     1981 cccgcgtcct tttctcggtc accgcaatca ataggcaatt tttcagttct cgctggtact
     2041 ggcccagatc gactcccgca aaaatcccct ttttcagcgc ggcacgaatg attttcctcg
     2101 ccggcacagg ggtttcgagg acgaattcct tgaaaaaagg tgacgcaaat ctcagccgga
     2161 agcccggaag tctggccaat tgatccacag catagtgcgc cttctgcagg cagagttcgg
     2221 caatgtcctt gatgcccgct ctccccaaga gtgaaaggta aactgcggct gccagcgcgc
     2281 agagcgcctg gttggtgcag atgttggagg tggccttctc gcgccggata tgctgttccc
     2341 tggtttgcag ggtgaggacg tacccgcgcc ggcctttggc atcgaccgtt tccgcggcga
     2401 tcctgcccgg catccggcgc accagttctt ttttgccggc gaaaaagcca aaacccggcc
     2461 cgcccaactg ggtgggaatg cccaggctct gcccttcccc caccgccaga tcggagttgt
     2521 actcgccggg cggcgcgagc aagccgagcg aaatcggatc gcagaccgca atcaatagcg
     2581 cccccgcctg gtggacgagc ggctcgaatt cggcgggctt ttctaagcaa ccgtagaagt
     2641 tgggctgctg gacaattacc gccgcggtgc ggtcggttat ttttttcttg agatcttcga
     2701 gatcgattgt gccttccgcg ccgcggacca tcgccaattt catagctccg ccgcgcgagt
     2761 aggtcgccag cactttctga tagtgcggat tcaaggtggc gggaaaaaga atttctttgc
     2821 gtccggtttg tccggtcgcc aggagcgccg cctcggccac agccgaaggg ccgtcgtaca
     2881 tcgaggcgtt ggaaacctcc atcccggtca actggcagat gagcgactga aattcgtaaa
     2941 tggcttgcaa cgtcccctgg gccacttcgg cctgggaggg tgtgtaggcg gtatagaatt
     3001 ccgggcggcg gagaatggcg tcgaccgcgg cggggacata gtggtcgtag atccccccgc
     3061 ccaggaacga aacgtactcc tggctggaac cattctctcc ggccagcgac cgcaaatgag
     3121 ccgccgtttc cagttcggag agtcttttgg gcaaagcgag cggtttttgt aaaacaagtt
     3181 ccggcggaag ttcgcgcagg aggtcctcaa agcggcccac gccgatgcgt tccagcatct
     3241 tccggcgatc gttgggtgta tgcggaaagt aagtcacgcg tttgcctgtc tttcggtcat
     3301 ggaacgaatc atttccctat tcagaatacg taacaggacg caatatagtc cgttgcggag
     3361 agtgtgccaa gcctaagtaa acctctctgc cgacccctcc ctcaccctcc tcggtacgag
     3421 tctatcgacc ctcaagggga gggaaaacct ccgaagatac cctgcgaagt tgagaggcaa
     3481 gcaaacgaga aaagctacgt cttaatcagc gcctcgtagc ggctggcgtc caggagcgcg
     3541 gctaattctt gactgttttt gacctttatt tttatgagcc acccctcacc gtaggggtct
     3601 ttgttgatga ttcccgcctc cgtttctatc cggacattca cctccgtgac ttcgccggat
     3661 aacggcgcgt acatttctga aaccgctttg accgcttcga tggttccaaa cggcttcatc
     3721 tgctctacgg cagtcccgac tttcgggagt tccacgaaca ccacgtcgcc caactctccc
     3781 tgcgcgtagt cggtgatacc caccgtggca atagcgccct cgactttgac ccactcgtgt
     3841 tctttggtgt acttcagttc cggtttaatc agcatgcgct cgaatcctct cctgttattt
     3901 gaggaagcgt cctcgctgac ccctccccgc cctcccctca aaggggaggg gaaaccagcc
     3961 accccttaat gaagaagagt tacggtaggg cgtcattaac tgtcacttta cgccgatgcc
     4021 tgaacgccct cgggtacggg agcggcgggt tcagaaacgg cggtcacccg ctcgactcct
     4081 aattcccgct gcagttcttc cacaaaggcg gtatcgtact gcccggccaa aaagcgctcg
     4141 tggtggagca ctttcaggtg aaacggaatc gtcgttttga ttccttcgat ggtgaattcc
     4201 tccagcgccc gccgcatccg gatgatcgct tcctcccgcg ttttgccgtg ggcgatcact
     4261 ttggccacca gcgaatcgta gaagggcggg atgacgtact tgacgtaggc gtgggtatcg
     4321 acccggatgc cgtgcccgcc gggaatgtga aaggcggaga gttccccggg gcagggcata
     4381 aaatcgcgct ccggatcttc ggcattgatg cggcactcca gggcgtggcc ggagagtttg
     4441 acgtccccct ggcgcaggga gagcttctct cccgcagcaa ctttgatttg ctccttgact
     4501 aagtcgatgt cggccacctc ttcggtcacc gggtgctcca cctgaattcg ggtattcatt
     4561 tccatgaaat agaaatgtcc ggtcgggtcg tagaggaatt caaccgttcc ggcgccccgg
     4621 tacttgatgt ttttcgcgcc gcggaccgcc gcctcagcca tctggtgcct ccgcctttcc
     4681 ggcaggatcg gacagggcga ctcctccaga agtttctggt gccgcctttg aatcgtgcag
     4741 tcgcgctccc cgaagtggat gacgttcccg tggtgatcgc ctaagatttg aatctcgacg
     4801 tggcgcggat tcgggagaaa tttttcgaga tagactgccg ggttcccgaa ggcggcctgc
     4861 gcttcggccc gggcggtgtc aaacatcgat ttcagatcgg acggatccat tgcgcagcgc
     4921 aggccccgcc cgccgccgcc cgccgccgct ttgatcatca acgggtagtc gatttcctcc
     4981 gccacctcca gcgcttcttc gagcgtgttc accggcccgt cactccccgg aatgacccgc
     5041 actccggctt tggccatggt gtttttggcg gcaagtttgt cgcccatcaa gcggatttgc
     5101 tggggtgtgg ggccgatgaa aaccaggttg caggcctgac agatttcggc gaagtcggcg
     5161 ttctcggcca aaaaaccgta gccgggatga atcgcgtcgg cgttggtcac ctccgcgacg
     5221 ctgatgatcc gcttgggatc taagtacgaa tccttcgcca gcccggggcc gatgcagacg
     5281 tcttcgtcgg cgaaccggac gtggagcgaa ttggcgtccg cctcggagtg caccgccacg
     5341 gtggcgatcc ctaactcgtt gcaggcgcgg ataacccgca gcgcaatctc cccccggttg
     5401 gcgattagaa ctttcttaaa catctccgct tctccccttc cacattgagg cgcaatataa
     5461 tacttcaacc gccgtcaacc agatgcattt ttcaatatat gagagagaaa ttgtgtttac
     5521 gcacgccgag gctggaagcc tcggctatgg aggctaaccc gtttttggcg gtgtggtgtt
     5581 aagaaaaagg cagcccgtac gggccgccta cgcgggtccg agatagtaat ctctctgcaa
     5641 agccctcaca cccgcggctt ctggctctgt tcaaactccg tccagccccg gtcggcggcg
     5701 ccggtgatgc ccttgacccg caggtcgaag tcgtccgggt tggaggtctg ggccatcgcc
     5761 tcttcgtagg aaatagcgcc ctggcggtag agcttcataa tcgactggtc gaacgactgc
     5821 atcccgtact gcaccgagcc agactcgatc agttcgataa tcgtagtggc tttcatggga
     5881 tcgatgatcg cctcccgcac cgaaagggtg ttgatgagca cctccgtcgc cggcacccgg
     5941 ccgggaatgt cggtgcgcgg cagaagtctc tggcagatca ccgccttgag cgtgctggct
     6001 aaaaggagcc ggatctgctg gtgctggtgc ggcgggaaga acgatatgat gcggctgatg
     6061 gtttccacgg cgttcagagt gtggagcgta gtcaagacca ggtgtccggt gtcggcggcg
     6121 gtgagggcaa tcgacatggt gtccagatcg cggatttcac cgatcagaat cacgtccggg
     6181 tcctggcgga aggcgtggcg caaggacgac gggaacgact cggtgtcacc gcccacttcg
     6241 cgctggctga tgatcgactg cttgtcgctg tagacgtact cgatcgggtc ctcgatggtc
     6301 agaatgtttt ccgcgcgggt ggcgttgatg tactcaatca tggcggcgag cgtggtcgat
     6361 ttcccgctgc cggtggtgcc ggtgacgacg atcatgccgc gccggttctc cgccagcttg
     6421 cagacgatct ccggcaggtt caactcttca aaactgggga tttcggtgga gacggcccgg
     6481 atggccaccc ccacggtgcc gcgctggcgg tagatattga tccggaagcg tcccatgttg
     6541 accaccgaga gcgccaagtc catctcgttg cgcttgagga aacgttcctt ctgcgggggg
     6601 gtgagaatct ggttcaagat ggcttcgagg ccttcgttgg tcaaaggctc ggcctccaag
     6661 ggcgtcaagc agccgtccac gcgcaaagtc gggcgcaaac ccacgcgcaa gtggaggtcg
     6721 gatgcgcgct ggctctgcat ctccgccagc atttgtttta aattcatcga ctcctcctcg
     6781 gatttttcat gaacggcgcc tggcggccgc caatccgggg aggaagtccc gccgcaggga
     6841 gactatcctg tgggttcgac gaaaaagagc ggctgtccga actccaccgg cctggcgttt
     6901 tcaaccaaaa ttttcactac ccgcccggcc acgtccgact cgatctcgtt catcagcttc
     6961 atggcctcga tgatacagac cacctggcct ttctcgatca cctggcccaa ttcgacgtac
     7021 gacttggcgt cgggagccgg ggcgcggtag aaggttccca ccatcggcga tttgatcgcc
     7081 gctactctat ctgtcggaat ttccggcgga acctccaccc cggcaacggc ggggttattc
     7141 ccggactgtg ccgcagtcaa aggcgtgaag tgcgaaggtc tttgggtggg tgccaatacg
     7201 tggaggtgcg ggagcgcggg cgctccgccg gaggcgggct ggccgttctc ggcggagagg
     7261 cgtttggtga ttttcacccg ccttccccaa ctccagattt ccagcgactc gatggcgctc
     7321 tgttcgacca ggcgaatcag cttgcgtaag gtctgttcgc gcataccgcc tcctctgccc
     7381 tgcgatctac cgtagggggt caatatactg aacccctaca aattccaaac cacgcgacgg
     7441 gttacaattc taacaaggtg cggtcggctt gggtcaacac ccggcagccg ccgtgggtaa
     7501 tgagcacgtc gtcttcaatc cgcaccccgc cccagcgggg gagatagact cccggctcga
     7561 tggtaaccac catgccggcc aaaagttttt cggtgctgcg gctgttgatc cccggcagct
     7621 ggtgcacctc caaccccagg ccgtgaccta acccgtgctg aaaatacttc cccaaccgcc
     7681 aatgtgcaat atacccccgg gcccgggcat ccaggtcttt ggcaacgata cctgcacgag
     7741 ccgcattgat tactcttttt tgcgcttcca aaacgatgcg gtaaattttt ttctgccggg
     7801 aggaggcccg gcccatgaca acggttcggg tgaggtcgga ggcgtagccg ttcacgactg
     7861 cgccgaagtc cagcgtgacg aaatcgcctt tttgaatttt ccggtccgaa gcccggccgt
     7921 ggggcagagc cgaccgcggg ccggaggcga cgatggtttc aaacgccgct ccttcggccc
     7981 cgtcctgttt catttgatac tccaattccg ccgccacctc ccgctccgca agccccggcc
     8041 gcaggaagtt cacgattttt tcaaagcaac gatcggcgat gccagcggct tgctctattg
     8101 cggcgatttc gccggcgtct tttatcgcgg cgacccgctc gactaaattt tccgtgggga
     8161 gagggagggc ctgcggcagg tgtttcttca gtagttccca gccccgtacg gtaacgtggc
     8221 ccgattcgca gcccattttc accttgggcg tgcggaggaa aggaatttgc gggaggagcg
     8281 caaacaaatc gccggtgccg atcacaattt tagcgccagg tacttcctgg tgtgcctgca
     8341 gatcgtagcg gtggtcggaa aagaaatagg tccggcgggg agtcaaaacg actaagccat
     8401 ttgatccggt aaagccggag agatagcgga cgttggcgcc ggaggtgacg acaagacagt
     8461 ccagccgttc cctggcgact gccgccagca ctttcatgcg ccgccgtaag agggtgtcga
     8521 ggtgtcggag tg
//
LOCUS       DBNGZD010000282         6148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1226548, whole genome shotgun sequence.
ACCESSION   DBNGZD010000282 DBNGZD010000000
VERSION     DBNGZD010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1226548"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="AC16GM_07530"
     CDS             complement(<1..541)
                     /locus_tag="AC16GM_07530"
                     /inference="COORDINATES: protein motif:HMM:NF028046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923561.1"
                     /translation="MRFGFRSKLIFSLVVLFALSFVASTVVVEKLVRNFWIGQLKKAL
                     EASARLLAFELTGPLQTPGYERLDQAADTYGQLLALRVTVIDTTGKVWGDTQVGGDTL
                     VTLENHASRPEVAAALSGKTGSSIRFSTTVKTEMLYLAYPISDGGHVVGVVRLARPLA
                     EVNEAIARIRRTILIFFFVV"
     gene            complement(538..1227)
                     /locus_tag="AC16GM_07535"
     CDS             complement(538..1227)
                     /locus_tag="AC16GM_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR2923562.1"
                     /translation="MKAKVILVVEDDPDIRELLARYLKREEYQVLLAKDGEEGLERAG
                     KVHPDLIILDLMLPRQDGLSVLKDLRRGEATTAIPVLILTAKGEEPDRIAGFEFGADD
                     YVTKPFSPREVAARVKALLRRVTQPGKPAEVQYLYGTLIVDTEQHKVFYEKEEVQLTA
                     KEFSLLKLFLDNRGKLLTRERILARVWGTDFSGGPRTVDVHIRRLREKIQLLEKSIAT
                     VKSYGYRLEEP"
     gene            1707..3137
                     /locus_tag="AC16GM_07540"
     CDS             1707..3137
                     /locus_tag="AC16GM_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR2923563.1"
                     /translation="MKKSFAISVVLGSLFLSTVASAQFITNYSFLGGGPRARGMGGAS
                     IGVADDPSTATWNPAGLTLLDRVQTGESFSYLRPRVKGEPSLTAGTRSFLNSFSSNKN
                     MGVINYASFILPGKVGGRQMVGALAFRRAEELFQDIALASDSTRYKRHLEGSLNVLNL
                     AVGTKITDAFAVGAGLNVYLGGYDRTEQINFTVTDTALYHQPYGAQQIFRYNWNQQSN
                     FRGVNFELSTLYLGPKYRLGAIVRTPFQLKDKFETRRGDTLFVDNVPNFFYSRGLFTP
                     RTATELGIPLSVGVGGSYKATEALTVAVDFEARLYSQADLSVPDSILDPASPLHSQNL
                     NWSDDFQVRFGLEYLLQSGGMKFPLRLGARNEPQIFEDARLLEAAASPYSSDVNNERL
                     DKGTPIFAGRTVRKQGIVGTAGAGLQMAQIHLDLTYELGTVKPQETGSYVDPSAPKTG
                     GVRSTTFTTAQDHLSHQVTFSFTGYF"
     gene            3257..3946
                     /locus_tag="AC16GM_07545"
     CDS             3257..3946
                     /locus_tag="AC16GM_07545"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2923564.1"
                     /translation="MSPEKRITKHQMKEDRLVTSVFIAFEYLQRNYQRLAIGIGAVLV
                     VILAVFLYNRGQKQKALEAEAACGQAQLRVTAGDLQGGILDLRRVIELYGGTAAGQKA
                     TYFLANAYLQARDYRQAEQLFRQFVAGGYKERLLVGSAHMGLGVCLEETGKTAEAAGE
                     YLKAVEISGKDYTTPEYALAAIRALAKTGQKEKALDLFNRLLNEFPRNFTDHNRAREL
                     LARYGIFDYGS"
     gene            3956..>6148
                     /locus_tag="AC16GM_07550"
     CDS             3956..>6148
                     /locus_tag="AC16GM_07550"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR2923565.1"
                     /translation="MSVRPIPGEGTGGKRPVPTCGEQSRTKGDKAGRAGRTPLSVALL
                     WHFHQPDYREPGTGTCRLPWVRLHGVKDYLDMLLRVEREPGLKVTFNWVPSLLEQLDD
                     YCSGRTSDPHLDLTRVPAALLSPEQKLAIVTDFFLGPPETMIKPHRRFWSLWLKKERA
                     AGDRDRARDSFTEEDWRDLQVWSNLAWFDPLFRRGTPLTPLFEKGERFSEADKQVMLD
                     YQFDILRQIVPTLKRLRAAGQIEVSFSPAYHPILPLLVDSRCAREALPDATLPDPPFR
                     HPEDAREQIRSACVLYEELFGERLQGLWPPEGGVSADLVPLVTRQGIGWIATDQKIYE
                     RSVSAGGEPGLGLYQPYRVGTGDQSLAVFFRDHTLSDLIGFAYARWDAERAVNDLLER
                     LHAIRAHLPEEALPRAAVSIILDGENAWEYYPEDGGRFLDLLYERLTGDPLLETITFG
                     EYLRRHPDLPALSRLAAGSWIYGNFNVWIGQPEDNRAWEFLRAARALLQAKELAGAPA
                     PQVEQARRTLSRAEGSDWFWWYGEDHRTEQKDIFDELFRAHLCEVYRALETSPPPDLL
                     LPIGGEPARRSEPPSQVIHPTIDGQITHFYEWSGAGLYRVGAGSMRPAHAGVTRRIWY
                     GLDETRLYLRCDFAKGVPVERLRTLRFRFDFQTQPPLAIELSGDRLTILPEQPPGTAL
                     ISGKVETILELALPLELLSVGADRRVPVSFSVYAEKVLLETWPAHGALA"
BASE COUNT         1261 a         1830 c         1761 g         1296 t
ORIGIN      
        1 agaccacgaa aaagaatatc agaatcgtgc gtcggattct ggctatcgcc tcattcacct
       61 ccgccagcgg ccgggcgaga cgcaccacgc ccactacgtg gccgccgtct gagattggat
      121 aggcaagata gagcatttcg gttttaactg tcgtggaaaa acggatagaa gaccccgttt
      181 tgcccgaaag ggccgcagcg acttcaggac ggctggcatg attttctaaa gtcacaagag
      241 tatccccgcc tacctgggta tcaccccaga cctttcccgt ggtgtcaatc acggtgaccc
      301 gcagggctaa gagctggccg taggtgtcgg cggcttgatc taaccgttca tatccgggag
      361 tctggagcgg tcccgtaagt tcgaacgcga gcagccgggc actggcctcc agcgcttttt
      421 tcaattgacc aatccaaaag ttacgcacca gtttttccac gacaacggta ctcgcaacga
      481 aggaaagcgc aaaaagcact actaaggaaa agatgagctt ggaccggaac ccgaatctca
      541 cggctcctcc aggcggtagc cgtacgattt caccgttgcg atcgattttt ccaagagctg
      601 gattttttca cgcaagcggc ggatgtgcac gtccacagtg cggggaccac cggaaaagtc
      661 tgttccccac acgcgggcga ggatgcgttc gcgcgtcaag agcttgccgc ggttatccaa
      721 aaagagtttc aacaggctga actctttagc cgtcagttgt acttcctctt tttcatagaa
      781 tactttgtgc tgctcggtgt ctacaatcag agtaccgtac aggtactgca cttcggcggg
      841 ttttcccggt tgagtgaccc ggcgcagcaa ggccttcacc cgcgcggcca cttcacgcgg
      901 ggaaaagggc ttggttacat agtcatccgc tccaaactca aacccggcaa tgcggtcggg
      961 ttcttcgccc ttggcggtca aaatcaaaac cggtatggca gtggtagctt ctccgcgacg
     1021 caggtctttg agtaccgaaa ggccgtcctg acgcggaagc atcaggtcta aaataatcaa
     1081 atcgggatga acctttcctg ctcgttccag accctcctca ccgtctttcg ccaaaagtac
     1141 ctggtactct tccctcttca ggtagcgtgc gagtaactcg cgaatatccg ggtcatcttc
     1201 gaccactaga atgactttgg ccttcacggt cctaaaggaa gggtagaaat tactttttta
     1261 caagaggaga tagttgattt ctgccgtgaa aacatgttag actgctacga cttttgctac
     1321 gaatcgccgc cccaaataga tcgcaccata cgataaagta tcgcactcta ccccatcatg
     1381 tggacagatt aaccgatcgc gtaccttttt ggatcttatt tacttatggc gtaacctaat
     1441 gtaactcaac aagtcggggt gggagggccc ggtcttctta tttagatttc ctaaaccgtt
     1501 ggccggccgt tcgaatcggc ccgggggtac ttcaaaagca gggattaggg ataagggatt
     1561 agagatgaac accaaaggaa aaaggcaaaa aatcgagttg acaaatcgcg gtgggtaggg
     1621 tagaatggag tgtttttgcg ggaagaacgg caacgcgtag gcggggtttc aaccccgctc
     1681 ttttgagtga gtggagggtt aattcaatga agaaatcgtt cgcaatcagt gtggtgctgg
     1741 gcagtctgtt tttgagtacg gtcgcttcgg cccagttcat aacgaactat agtttcctcg
     1801 gcggcggtcc gcgcgcccgc ggtatgggcg gggcctccat cggcgtggcc gacgatccct
     1861 cgaccgccac ctggaacccg gccggcctca cccttttgga tcgggtgcag accggcgagt
     1921 cgttctccta cctgcgtccg cgggtcaaag gcgagccgtc cctgacggcg ggcacgcgtt
     1981 cgtttttgaa ttcgttctct tcaaacaaaa atatgggcgt aatcaactac gccagtttca
     2041 ttctccccgg caaagtgggc ggccggcaaa tggtgggcgc tctggctttc cggcgcgccg
     2101 aagaactgtt tcaggacatt gcgctggcca gcgacagcac cagatataaa cgccacctcg
     2161 aagggagtct gaacgtcttg aatctggcgg tggggaccaa aatcaccgat gcttttgcgg
     2221 tgggtgccgg gttaaacgtt tacctcggcg gctatgaccg taccgaacag atcaacttca
     2281 ccgtcaccga caccgcgctc taccatcaac cttacggagc ccagcagatt ttccggtaca
     2341 actggaacca gcagtctaac ttccgcggcg tgaactttga actctcgacc ctctacctgg
     2401 ggcccaagta ccggctgggg gccatcgtcc gcactccgtt ccagttgaag gataagttcg
     2461 aaacccggcg tggggacacg ctcttcgtcg ataacgtccc caatttcttt tactcccgcg
     2521 gcctcttcac cccccgcacg gcaacggaat taggcatacc cctctcggtc ggcgtgggcg
     2581 gctcctacaa agccaccgag gccttgaccg tcgcggtcga tttcgaggcg cgcctttact
     2641 cccaggccga cctctcggta cccgactcga ttttagatcc ggcgtctccg ctgcacagcc
     2701 aaaacctgaa ttggtccgac gactttcagg tgcgtttcgg ccttgagtat cttttgcaga
     2761 gtggaggaat gaagttcccc ctgcggctcg gcgcgcggaa tgagccgcag atttttgaag
     2821 atgcgcgtct cttggaagcg gccgcatctc cctactcttc tgatgtcaat aacgagcgcc
     2881 tggacaaagg taccccgatt ttcgccggcc gcaccgtgcg gaaacagggg atcgtaggca
     2941 cggccggagc cggtctgcaa atggcccaga tccacctcga cctgacctat gaactcggta
     3001 ccgtaaaacc ccaggagacc ggctcgtatg tcgatcccag cgccccgaaa acgggaggag
     3061 tgcggagcac gaccttcact acggcacagg atcacctgtc gcaccaggtc accttcagtt
     3121 tcaccgggta tttttaaatg ccggatttcc cgtggcgccc cgccaacggc gttccggtca
     3181 gttttgtctg gttgtgcgtt catccggttt tgactcttcc gaccaagcag atgtcatctg
     3241 gttttaagcg gcaaccatga gtcccgaaaa gcggattacc aagcaccaaa tgaaagagga
     3301 ccgattggtc acgtcggtct tcatcgcttt tgaatacctc cagcggaact accagcggct
     3361 ggccatcggg attggcgccg tgctcgtcgt gatcttggcc gtcttccttt acaaccgggg
     3421 acagaagcaa aaagcgctgg aggccgaagc cgcctgcggt caggcccagc tgcgggtcac
     3481 cgccggcgac ctccagggcg gcattctcga cctccgccgg gtgatagagc tgtatggcgg
     3541 tacggcggcg ggccagaagg cgacctactt tctggcgaac gcctacctgc aagccagaga
     3601 ctacaggcag gccgagcaac tctttcggca gtttgtcgcc ggcgggtaca aggaacgctt
     3661 gctggttggc tccgcacata tgggtctggg cgtctgcctg gaggaaacgg gcaaaaccgc
     3721 cgaggcagcc ggagagtacc tgaaagcggt ggaaatatcc gggaaagatt acaccacgcc
     3781 ggagtacgcg ctggccgcca tccgcgctct ggccaagacg gggcagaagg agaaagcact
     3841 cgatttattc aaccggctgt tgaacgagtt cccgcggaat ttcaccgatc acaaccgggc
     3901 ccgcgaactt ctggcccgct acggcatttt cgactacgga agttaactga ccggcatgtc
     3961 cgtccggccc attcctggtg aagggactgg tggcaagcga cctgtcccga cttgtggtga
     4021 gcaaagtcga accaagggag acaaagcggg ccgagccggc cgcaccccgc tttcggtcgc
     4081 ccttttgtgg cactttcacc agcccgacta ccgcgagccc gggacgggca cctgccgcct
     4141 cccctgggtg cgcctgcatg gtgtaaaaga ctaccttgat atgctcctgc gggtcgaacg
     4201 agagccgggt ttgaaggtga ccttcaactg ggtcccgtct cttttggaac agctcgacga
     4261 ttactgttcc ggaaggacat ccgaccctca tctggacttg acccgtgtac cggcagccct
     4321 actttccccc gaacagaagc tggcaattgt gaccgacttc ttcctcggac cgccggagac
     4381 gatgatcaag ccacaccggc gcttctggtc gctgtggctc aaaaaagagc gtgccgcggg
     4441 tgatcgggat cgggcgcggg actctttcac cgaagaagac tggcgcgacc tgcaggtctg
     4501 gagcaacctc gcctggtttg atcccttatt tcgccggggt acaccgttga cgcctctttt
     4561 cgagaaaggt gagcgtttca gcgaagccga caaacaggtg atgctcgatt atcagttcga
     4621 catcttgcgg cagattgtgc ccacgctcaa gcgcttgcgt gccgccgggc agattgaagt
     4681 ttctttttcg cccgcttatc acccgatctt gccgcttttg gtggactccc gctgcgcccg
     4741 agaagcgctg ccggatgcga cactgcccga tccccctttc cggcatccgg aagatgcgag
     4801 agagcaaatc cggtcggctt gcgtgctcta tgaagaactc ttcggtgaac gcctccaggg
     4861 cctctggccg ccggagggag gcgtctccgc cgatctggtt ccgctcgtta cccgacaggg
     4921 aatcggctgg atcgctactg atcaaaagat ttatgagcgc tcggtctcag ccggcggaga
     4981 acccggattg ggactatacc aaccctaccg ggtcgggacg ggtgaccaga gcctggccgt
     5041 cttcttccgt gaccacaccc tgtccgatct tatcggtttc gcctacgccc gctgggatgc
     5101 ggagcgcgcg gtcaatgact tgctggagcg tttgcacgcc atccgggctc acctgccgga
     5161 agaggcattg ccgcgggctg cggtctcgat catcctggac ggcgaaaatg cctgggagta
     5221 ctatccggag gacggcgggc ggtttctcga tttgctctac gaacggctga ccggcgatcc
     5281 gcttttggaa acgattacgt tcggagagta cttgcgccgc catcccgacc tgccagccct
     5341 ctcgcgactg gccgccggat cgtggatcta cggaaatttt aacgtctgga tcggccagcc
     5401 cgaggacaac cgggcctggg agtttttgcg cgcggctcgg gcgctcttgc aggcgaaaga
     5461 actggccggg gctcccgctc cgcaggtgga gcaagcgcgc cgcacgctgt cccgagccga
     5521 aggcagcgac tggttctggt ggtacggcga agaccaccgc acggaacaga aagacatttt
     5581 cgatgaactc ttccgtgccc acctgtgcga agtgtaccgg gcgctggaaa cctcgccgcc
     5641 gccggatctt ttgcttccta tcggcggcga gcccgcgcgt cggagcgagc cgccgtcgca
     5701 ggtcattcat cccacgattg acggtcagat cacccatttc tacgagtgga gcggcgccgg
     5761 cctctaccgt gtcggcgccg ggagcatgcg ccccgcgcac gccggggtga cgaggcgcat
     5821 ctggtacggc ctggatgaaa ccaggctcta cctccgctgc gattttgcca aaggtgttcc
     5881 ggtggaacgc ctccgaaccc tgcgtttccg cttcgacttc caaacgcagc ccccgctggc
     5941 catagaacta tccggcgacc ggctcaccat cctgcccgaa caaccgccgg ggaccgcttt
     6001 gatctccggg aaagtcgaaa ccatattgga actcgctctt ccgttggaac ttttgagcgt
     6061 cggcgcggac cgtagggtgc cggtgagctt ttcggtttat gccgaaaagg tgctactgga
     6121 aacctggccg gcccacggcg ccctggcc
//
LOCUS       DBNGZD010000283         5547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1852056, whole genome shotgun sequence.
ACCESSION   DBNGZD010000283 DBNGZD010000000
VERSION     DBNGZD010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1852056"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="AC16GM_07555"
     CDS             <1..507
                     /locus_tag="AC16GM_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2923566.1"
                     /translation="GAKPAGEHWPAPAGHGLVPAGDLLTNEGTVTAAIGCGREMALRL
                     HEEWTGQPILPPKASPEEVIRADQIRFHLFTHSLKNREHATPVDERRHTFKEVHRGLD
                     SYEEARRCLSCGVCNACDLCLSACPDGVVLRVNGELVFDYDYCKGCGVCVSECPRAVI
                     YMKTVGQE"
     gene            513..1685
                     /gene="porA"
                     /locus_tag="AC16GM_07560"
     CDS             513..1685
                     /gene="porA"
                     /locus_tag="AC16GM_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HIR2923567.1"
                     /translation="MSIEVVTGNHAAGHALSAAGEANRNARGVVCGIYPITPQTEIVE
                     HVANFHFTKGHVVAVESEHSAMGVCIGASLNGARSFTASSSNGLAYMAENILVAGFYR
                     LPILMVAVNRTLGPPWNIWADQGDTLMFRDAAWIQLYCEDNQEVVDSILLGFRLAEDK
                     RVLLPVMVNMDAFIISHTSMETDLPSQELVDSFLPHFELPHRIDVNRPVTIGGLNWPL
                     ETASSRVEMDQAMRLVPGIFEEHRRAFERQFGRPVHGALEAYSTDDADLILVASGSIA
                     TTARKVVQQRRATGEKIGLVKIKMFRPFPDSELRQITQRTSKIGVLDRNYSAGTGGIF
                     WQEIRASLQGKPGTLIQNYLTGLGGMDVTPQIISEVIDDLRGRSEMGLPVWKGVAV"
     gene            1682..2554
                     /locus_tag="AC16GM_07565"
     CDS             1682..2554
                     /locus_tag="AC16GM_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR2923568.1"
                     /translation="MTTYPECLLRPGNTNCGGCGMSSALTMLGRVLADQPSMMVIPAC
                     CGIVAAGAYPTTSYGVPVIAATFASAPAFASGVVAAARMNGDPMNVICWAGDGGTYDI
                     GLATLSAAAERNEDVIYFCYDNEIYGNTGGQRSSATPLYARTATTPAGKTEPKKELVE
                     IMVAHRIPYAATLSIAHKEDFENKIRTALGITGFRFLLIHSPCPIGWKSEPAESIQLV
                     RLAVQSGLFPLYEVFNGLEYRLTLEPDWSDPSPYFEKQRRFDGYRIDVAQVVQQARKQ
                     YERLRSLAGLTAGK"
     gene            2625..2891
                     /locus_tag="AC16GM_07570"
     CDS             2625..2891
                     /locus_tag="AC16GM_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923569.1"
                     /translation="METATLPVQELRRRHGLHANQLYRWKQALGRSLGDSGSGGSEAA
                     GLGRRLQPNRTARRDGNEIPHHILYGLDVKNKHYTCLNLGGAVH"
     gene            3389..3895
                     /locus_tag="AC16GM_07575"
     CDS             3389..3895
                     /locus_tag="AC16GM_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2923570.1"
                     /translation="MRLKWKILLLTLAVLLYGWWELKSVTSPLGYAPEQPLPFSHKLH
                     AGENKIPCLYCHANAERSRHATVPSMNICMGCHQVVATSKPAIVKLTEMYKKGESPEW
                     IRVHSVPDFGYFSHRWHVAKGIACQTCHGPVEQMERVVQSRLLKMGDCVTCHRQNNAP
                     TDCNTCHN"
     gene            3909..>5547
                     /locus_tag="AC16GM_07580"
     CDS             3909..>5547
                     /locus_tag="AC16GM_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR2923571.1"
                     /translation="MSDADEQKKHFWSTLEEWEGDPGLEQIRQEEFFSKPQAFFEAAQ
                     SQPVTLLNRRTMLKMTGAGMLFAAAACARRPVQKIVPYLNAPEEIIPGNAVWYASTCG
                     CPAGCGVLVKTREGRPIKLEGNPRHPLNRGRLSARCQAAVWDLYDPDRLSGPTQVFRS
                     KLPAAPLDWKTADARIAEALKSANGRVVLLTGTLHGPARERLIADFLASFPGARHITY
                     DALSEEQIADAQETCYGKPVVPRYRFNLAETVVFLGADPLASGHSVTEFAAGFGDRRK
                     VQNGSMSRVIAFEPALSLSGSNADSHFLVTPEDLLPVALGLAHQLVIAEGKSAYARDP
                     MVRSALAPFAPEKVERETGLPAGTLRSVAADLWNARGKSLIYTGGLATQTGNGTALHI
                     AANFLNSLLENEGTTVDGTQSPSHQTRGSQAAMLELIADLQAGKVDVLLIYGTNPAYS
                     LPPSAGLAQALYRVKLAVSLNDRIDETGALCDYVLPSLHFLESWGDAEPQKGLFSLTQ
                     PAIGPLHDTRGFEESLLALARQAGAGNLGKWAGTWHDY"
BASE COUNT         1205 a         1570 c         1603 g         1169 t
ORIGIN      
        1 ggagccaagc cggccggcga gcactggccg gcaccggctg gccacggcct tgttcccgcc
       61 ggcgatctcc tgaccaacga aggaaccgta accgcggcca taggctgcgg ccgcgagatg
      121 gcgctccgct tgcacgaaga atggacgggt caaccgattt tgccgcccaa agcgagccct
      181 gaagaagtca tccgggctga ccagatcaga ttccatcttt tcactcattc cctgaaaaat
      241 cgagaacatg caactccagt ggatgaacgt cggcacacct ttaaggaagt gcaccggggc
      301 ctggacagct acgaagaagc gcgtcgatgt ctgagctgtg gagtttgcaa tgcctgcgac
      361 ctctgtctta gtgcttgtcc ggatggtgtg gtgttaagag tgaatggaga actggtgttt
      421 gattatgact attgcaaagg atgcggcgtg tgcgtgtccg aatgtccgcg cgcagtcatt
      481 tacatgaaaa cggttggaca ggagtaaccc ggatgtcgat agaagtagtc actgggaatc
      541 acgcggccgg ccatgcgtta agcgccgccg gcgaagcaaa ccgcaacgca cggggagtcg
      601 tctgtggaat ctacccaatt acacctcaaa ctgaaatcgt tgaacacgtg gcgaatttcc
      661 actttaccaa ggggcatgtt gtcgcggtcg agagtgaaca tagcgccatg ggcgtctgca
      721 ttggcgcatc gctgaacgga gcgcgctcgt ttaccgcaag ttcttccaac ggcctggcct
      781 atatggcgga gaatatcctg gtggctggtt tctatcgact acccatactg atggtggcgg
      841 tcaatcggac gctcgggccg ccgtggaaca tttgggccga ccagggggat acgcttatgt
      901 tccgagatgc cgcctggatt cagctctatt gcgaagacaa tcaggaagtg gtcgattcca
      961 ttctcttagg ttttcggctg gctgaagata aaagggtatt gctgccggtg atggtcaata
     1021 tggacgcctt tattatttcg catacatcga tggaaactga cttgcccagt caggaattgg
     1081 ttgattcgtt cctcccgcat ttcgaactgc ctcatcgaat tgatgtcaat cgtccggtaa
     1141 cgattggagg tttgaattgg ccacttgaga cagcgtcatc gcgcgtggaa atggaccagg
     1201 ccatgcgcct ggtccccggc atttttgaag agcatcgccg cgcattcgaa cggcaattcg
     1261 gccgacctgt tcacggcgcc ttggaagcgt actccaccga cgatgccgac ctgatacttg
     1321 tcgcatcggg gagcatcgct accaccgccc ggaaagtcgt ccagcaacgt cgtgccaccg
     1381 gtgagaagat cggcctcgtt aagattaaga tgtttcgtcc ctttcccgat tcggaattga
     1441 ggcaaatcac ccaacgaacg tcaaagatag gcgtccttga ccgtaattat tcagccggaa
     1501 ccggcggaat attctggcag gaaatccggg cctcgcttca gggaaaaccg ggcactctga
     1561 tacaaaatta cttaacgggt ctgggaggaa tggacgttac cccgcaaata atttctgagg
     1621 ttattgacga ccttcgcgga agatccgaaa tggggctccc ggtctggaaa ggagtggcgg
     1681 tatgacaacg tatccggagt gcctgcttcg tccgggaaac accaattgcg gcggctgcgg
     1741 gatgagcagc gctttgacga tgctgggacg ggtgctggcc gaccagccca gtatgatggt
     1801 tattcccgcc tgctgcggca tcgttgctgc cggtgcgtat cctaccacca gctatggagt
     1861 gcctgttatc gccgctacat ttgcgtcagc gccggccttt gccagcggtg tagttgcggc
     1921 cgcccgcatg aatggagacc cgatgaatgt catttgctgg gccggtgacg gcggcactta
     1981 cgacataggc ctggccactc tgtcggcggc cgcggagcgg aatgaagatg ttatttattt
     2041 ctgctacgat aacgaaatct atgggaatac cggtggacag agaagttcgg ctacgccgct
     2101 ttatgccaga accgctacga cgccagcagg taaaacggaa ccgaaaaaag aacttgttga
     2161 gattatggtc gcgcatcgca ttccctatgc cgctaccctt tcaatagcgc acaaggagga
     2221 cttcgaaaac aaaatacgta cagctctggg catcaccggg tttcgttttc tgctgataca
     2281 ctccccctgt cctatcggtt ggaaatcgga gccggctgaa tcgatacaac tggtacgtct
     2341 ggccgtacaa tcaggtctgt ttccactgta tgaagttttc aacgggcttg aatatcgcct
     2401 gacattggaa ccggactggt cggaccccag tccctacttt gaaaagcaac gccgttttga
     2461 tggttaccgg atagatgttg cacaagtcgt gcaacaagca cggaaacaat acgagcgact
     2521 gcgcagtctg gccggtttga cagcaggcaa atgacttctt ttgcaaatag gattgccgtg
     2581 ccccacttga gatttggaca ggtataatgg ggtaaggagg tttgatggaa acagcgacgc
     2641 tgccggttca agagctccgc cggcgccacg gtttgcacgc caaccagttg taccgctgga
     2701 agcaggcttt ggggcggagt ttgggggatt cgggaagcgg tggctcggaa gctgccggac
     2761 tgggtcgaag actacaaccg aatcgcaccg cacggcgcga tgggaatgaa attccccatc
     2821 atattctata cggattggat gtcaaaaata agcactacac ctgtctaaat ttaggaggag
     2881 cagtacacta agaaaagaaa tgccatattg ccagatcact tatccaaaat aaaatcccga
     2941 ctcatgaacc ggcaacaccg gcgtaaactt cgccctttac ccacttcgct ttccacttcc
     3001 acccggcccg tgagtcctct cgccattatt ggtttgtact acggtgctaa aatgacaccc
     3061 tcactcgcgc cgccccgcct ttggcccggc aaggcgccac gctcgtcgtt cgcccgcctg
     3121 aggcgggctc actacggggt tgctttccgg atgcggccgc tttagattct cccgactgag
     3181 cgaagagaat caccgggcag cctattttga cgagagaaag ctgttgacaa aaaagaattg
     3241 atattatagc gtacccacgt ttgtgaaaat aatcacaagt gagcgggttt ggccgggccg
     3301 gcggcacaac atcattaaag tagcaggctg agaagggtat tggcgctcgt tttgtcggca
     3361 ctgcgccggt ggagaaattt ttgtagttat gcgattgaag tggaaaatac tcctgttaac
     3421 acttgccgta cttctctacg gctggtggga gttgaaatcg gtcacttcgc ctttgggcta
     3481 cgctccggag cagccgctac ctttcagcca caaactccac gccggagaga acaagatccc
     3541 ctgcctctac tgccatgcca atgcggagcg ctcgcgccac gccaccgtgc cctcgatgaa
     3601 tatctgcatg ggctgccatc aggtggtggc gacctccaaa cccgctattg tgaagttgac
     3661 cgagatgtac aagaagggtg agtcgccgga atggatccgc gttcacagcg tgccggactt
     3721 cggctacttt tcgcaccggt ggcacgtcgc caaggggatt gcctgccaga cctgccacgg
     3781 gccggtggaa cagatggagc gggtggttca gtcccggctc ttgaagatgg gcgactgcgt
     3841 cacctgccac cgccagaaca acgcgcccac ggattgcaat acctgccata attgagcgag
     3901 agcatttgat gagcgacgcc gacgaacaaa aaaagcattt ctggagcacg ctggaggagt
     3961 gggaaggcga tcccggcctg gagcagatcc ggcaggagga gtttttctcc aagccgcagg
     4021 ccttcttcga ggcggcccaa tcccagccgg tgacgctttt gaaccggcgg acgatgctca
     4081 agatgaccgg cgcgggaatg ctgttcgcgg ctgcggcctg cgcccggcgc ccggtgcaaa
     4141 aaatagtacc ctacctgaat gccccggaag aaataatccc ggggaacgcg gtctggtacg
     4201 cctccacctg cggctgtccg gcgggctgcg gtgttttggt gaaaacccgc gagggacgcc
     4261 cgatcaaact cgaaggcaac ccccgccacc cgctcaaccg gggacgcctc tcggcccgct
     4321 gtcaggcggc agtgtgggac ctgtacgacc ccgaccgact cagcggaccg acgcaggttt
     4381 ttcgctcgaa actccccgcc gcgccgcttg attggaaaac tgccgatgcc agaatcgctg
     4441 aggcgctgaa atcagccaat ggccgggtgg tgctcttgac cggcaccctc cacggcccgg
     4501 cccgggagcg gctgatcgcc gacttcctcg catctttccc gggggcccgt cacattacct
     4561 acgacgcgct ttccgaagaa caaatcgcgg acgctcagga aacctgctat ggcaaaccgg
     4621 tcgtgccccg ctaccgcttc aatctggcgg aaacggtggt tttcctcggc gccgatcccc
     4681 tggcgagcgg ccattcggtg accgaatttg ccgccggctt tggcgaccgc cggaaagtgc
     4741 agaacggctc aatgtcgcgg gtgattgctt tcgaaccggc gctgtcgctc tccggctcca
     4801 acgccgacag tcactttttg gttacaccgg aggatttatt acccgtcgcg ctggggctgg
     4861 cgcatcagtt agtcatcgct gagggcaagt cggcctacgc ccgtgaccct atggtgcgga
     4921 gcgctctcgc tcctttcgct ccggagaaag tcgaaaggga aaccggcctg cctgcgggga
     4981 cactccgctc cgtagcggca gacctctgga atgcgcgcgg gaaaagtttg atctacaccg
     5041 gcggcctggc gacgcaaacc gggaacggta ccgcgcttca cattgccgcc aatttcctca
     5101 actcactgct cgaaaacgaa ggcacaaccg tagacggcac gcagagtccc agccaccaga
     5161 cccgcggttc gcaggccgcc atgctggaac tgattgccga tctgcaggcg ggcaaggttg
     5221 acgtgctttt gatctacgga acgaatcccg cctattcttt gcccccctcg gctggtttgg
     5281 cccaggccct ctatcgggtg aagctggccg tttccttgaa tgaccggatt gacgaaaccg
     5341 gtgctttatg tgactacgtt ctgccttcgc tccacttcct tgaatcctgg ggcgacgccg
     5401 agccgcagaa aggtttattc agtctgaccc agcccgccat cggccccctg cacgacacgc
     5461 gcgggtttga agaatcgctt ctggctttgg ccaggcaggc gggtgcgggg aacctcggaa
     5521 aatgggctgg aacctggcac gactacc
//
LOCUS       DBNGZD010000284         3360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_810405, whole genome shotgun sequence.
ACCESSION   DBNGZD010000284 DBNGZD010000000
VERSION     DBNGZD010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_810405"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="AC16GM_07585"
     CDS             complement(<1..567)
                     /locus_tag="AC16GM_07585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR2923572.1"
                     /translation="MSYPLIGITCGAVPEAAGKRKPTRLAYDFLKRQYYEAVEAAGGT
                     PVLLPNSQNPETVAALVERLDGILFSGGEDVDPSFYGEQTKAKNLQLAAQRDQFELQL
                     IKAVPEQRLPVLGICRGHQVLNVGRGGSLFQDLALRSEPTLNHAQGGSGTYLVRHGVE
                     VKKGTRLFAIVKSEHIDVNTSHHQMVKTV"
     gene            complement(586..2511)
                     /locus_tag="AC16GM_07590"
     CDS             complement(586..2511)
                     /locus_tag="AC16GM_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR2923573.1"
                     /translation="MRAFRLVVVTFFWFTGAAVAQVPPRDWQEDFSWRGAERPKIGKI
                     YLTGNQKFPTGLLREKLFSREASWLVSFGLARATRLQKDSRERDLVNLLYSYQSNGFL
                     EARITERFGWDSTARRVDIWVEIGEGVQTKIGPVTVAGDTAGFSEKIFKLTTAMKSGE
                     PFNPFVLEQTRSGVKTLLADNGYPYARVSVDTLRDSTLPNLGIQFRIARGQEVHFGRV
                     TVEGLQYTRPHVATRELVFHPGDRFSREKIVSSQQRIYSTGLFTFVNLVPQSSGAGGD
                     SASRAQPAQPDFILKLVERKPAFFGFKAGAGQYQQQEQQQDLAVDLGTQWGNRNLWGT
                     ARKYNIELKTSFLVINRFRILNNRIAGAYQEPWFLGTRTAANFTLSFEPGVQSAVLPY
                     RIETITGGLSLVRELSLTTRLLLTGLFQHVAIFGITPENILAFKRDQGITIRRRVILS
                     FERDTRDNIFIPLSGAQTYLSGELTGGPFGGDAEFFKLVANWARYRSLSGSNVFAVRV
                     KAGYLEGLRAGSFIPASDRFFLGGGSSLRGYTENSLGPLSASGDPLGGRTLLLANGEL
                     RRPLFWKFWGSMFADVGNIWERPVDFSLGDLRITTGAGLQFLTVVGPIRIDYGRRVVR
                     GAAQPGGRVHFSILYAF"
     gene            complement(2559..>3360)
                     /locus_tag="AC16GM_07595"
     CDS             complement(2559..>3360)
                     /locus_tag="AC16GM_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR2923574.1"
                     /translation="LGGKTVSPPWDLQLTVDLPGNFRVTNEETNTEFKGKLVVLRENG
                     IYNYLGELETVRGKFYTIGRTFTITQGKITYDNISVPDPKLDITAVTRIRRFERDTAF
                     TSSRPADIDLVIQITNTLSHPVIKPASGSPFSEEDLVLLLGTGGVFNPAATSSGDRKT
                     GISNRLTIGGVNFASAVLSQYAARQFGIETLELSPVSAGQFDLRQTELLVGKYTFPNF
                     YLYGRSRFASLKEQEVGFEYRINRWLSLEGVRDRANLYRFNLNANWEY"
BASE COUNT          702 a          987 c          990 g          681 t
ORIGIN      
        1 tacggttttc accatctggt ggtggctggt gttgacgtct atatgctcgg acttcacaat
       61 ggcaaacaag cgtgtccctt tcttcacttc aacgccgtgc ctcaccaagt aggttccact
      121 gcctccctga gcgtggttga gcgtcggctc ggagcggagg gctaaatcct ggaaaagcga
      181 gccgccccgg ccgacattga gcacctgatg cccgcggcaa atgccgagca ccggtagccg
      241 ttgctcaggg actgccttga tcaattgcag ttcaaattgg tctcgttggg ctgccagttg
      301 gagatttttt gctttcgtct gttcgccgta aaaagaagga tcgacatctt ccccaccgct
      361 gaagaggatg ccgtccaaac gttcgaccag cgccgcgacc gtttctggat tctgactgtt
      421 gggcaaaaga accggtgtgc cgccggcggc ttccaccgct tcgtagtact gccgtttgag
      481 gaagtcgtaa gccagacggg tcggcttacg cttcccggcc gcttccggga ctgctccaca
      541 ggtgatgccg atcaaaggat aggacattgg tcttttctgc tccgttcaga aagcgtacag
      601 aatgctgaag tgcacccggc cgcccggctg cgccgcgccg cgcaccaccc ggcggccgta
      661 gtcgatgcgg atcggcccca cgacagtcaa aaactgcaac cccgcgccgg tggtgatgcg
      721 cagatcgccc agggagaaat cgacgggcct ttcccagata ttcccgacat cggcaaacat
      781 actcccccag aacttccaga acaacggccg acgcagttcg ccgttggcca aaaggagggt
      841 ccgtccaccc aaagggtctc cactggccga caaggggcct agactgttct ctgtataccc
      901 ccgcaggctc gacccgccgc ccaaaaaaaa tcggtcgctc gccgggatga aacttcctgc
      961 ccgcagccct tcgaggtagc cggctttgac ccgcacagcg aaaacgttgc tgccggaaag
     1021 tgatcggtag cgtgcccagt tggccacaag cttgaagaac tccgcatccc cgccaaacgg
     1081 tccgccggtg agttcccctg aaaggtaggt ctgcgccccg gagagcggga tgaatatgtt
     1141 gtcacgggta tcccgctcaa aagagagaat cacccggcgc cggatggtga ttccctgatc
     1201 gcgtttgaag gccaggatgt tctccggcgt aatcccgaaa atggccacgt gctgaaacag
     1261 gccggtcaag agcaaacggg tggtcagcga caattcgcgc accagggaaa ggccgccggt
     1321 gatcgtttcg atccggtagg gcaggaccgc gctctgcacc cccggctcga acgagagggt
     1381 aaagttggcg gccgtccgcg tgcccaaaaa ccacggctcc tggtaggccc cggcaatccg
     1441 attgtttaat atccggaagc ggttgatgac gagaaaactg gtcttaagtt ctatgttgta
     1501 tttacgcgcc gtcccccaga ggttgcggtt gccccactgg gtccccaggt caaccgccag
     1561 gtcctgttgc tgctcctgct gctggtactg gccggctccg gctttgaacc cgaagaaagc
     1621 cggtttccgt tccaccagtt tcaagatgaa atcgggctga gccggctgcg cgcgggaggc
     1681 agaatcgccc ccggctccgg aagactgggg caccaaattg acaaaggtga aaaggccggt
     1741 ggagtaaatc cgctgctggg aagagacaat tttttcccgg ctgaagcggt cgcccggatg
     1801 gaaaaccagt tcccgcgtgg ccacgtgcgg ccgggtgtac tggagccctt ccaccgtcac
     1861 ccttccgaag tggacttcct gcccccgggc aatccggaat tgtatcccca gattcggcaa
     1921 ggtgctgtcg cgcagagtgt cgaccgaaac gcgggcgtac gggtagccgt tatcggccaa
     1981 caaggttttg acgccgctgc gggtttgttc cagcacaaaa gggttgaagg gctctcccga
     2041 tttcatggca gtagtgagtt tgaaaatttt ctccgagaaa cccgccgtat cgccggcgac
     2101 agtgaccggg ccgattttcg tctgcacacc ttcaccaatc tccacccaga tatcgacccg
     2161 ccgggccgtc gagtcccagc cgaatcgttc ggtgatgcgc gcctccagaa agccgttgct
     2221 ctggtaggag tacagaaggt tgaccagatc ccgctcccgg gaatctttct ggagtctcgt
     2281 tgcccgggcc agaccaaagg agacgagcca gctcgcttcc cgggaaaaga gtttttcacg
     2341 caggagaccg gtcgggaact tttgattgcc ggtgaggtag atctttccga ttttcgggcg
     2401 ctcggccccg cgccaagaaa aatcttcctg ccagtcacgc ggcggcactt gcgctactgc
     2461 cgctccggtg aaccagaaga acgtaactac gaccagccga aaggcgcgca cccgtgtcag
     2521 aatagaataa aggcggaaaa ctggcaacgg ccggggcttt agtactccca gttggcgttc
     2581 aggttgaagc ggtacaaatt ggcgcggtcg cgcacccctt ccaaggagag ccagcgattg
     2641 atccggtact caaaacccac ttcctgttct ttgagcgagg caaaacgcga gcggccgtac
     2701 aggtagaagt tcggaaaggt gtacttgccg accaaaagtt cggtctgccg caggtcgaac
     2761 tgtccggcgg ataccggcga aagttccagc gtctcgatcc cgaattgccg cgccgcatac
     2821 tggctcaaaa ctgccgatgc gaaattgacg ccgccgatgg tcagccggtt tgaaatcccg
     2881 gttttccggt cgccggagga ggtcgccgcc ggattgaaca ctcccccggt gcccaggagg
     2941 agcaccagat cttcttcgga gaaaggcgac ccggatgccg gtttaatgac cgggtggctc
     3001 aaggtattag tgatttgaat cacgagatca atgtctgccg gccgggagga ggtaaaggcc
     3061 gtgtcgcgtt cgaaccggcg gatgcgggtc acggccgtaa tgtccaattt cgggtcgggc
     3121 acgctgatgt tatcgtaggt gatcttcccc tgggtgatgg tgaaggtgcg gcctatcgtg
     3181 tagaatttcc cgcggacggt ttccagttca cctaagtaat tatagatgcc gttctcacgc
     3241 aagacgacca gtttcccttt gaattcggta ttggtttcct cgttggtcac ccgaaaattg
     3301 cccggaagat caacggtgag ctgcaaatcc cagggcgggc tgaccgtttt tccgcctaag
//
LOCUS       DBNGZD010000285         3443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1435864, whole genome shotgun sequence.
ACCESSION   DBNGZD010000285 DBNGZD010000000
VERSION     DBNGZD010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1435864"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16GM_07600"
     CDS             <1..315
                     /locus_tag="AC16GM_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2923575.1"
                     /translation="LILPGLVFRPLPEKFTLGTNFPNPFGSQTQLPFAVQEESRVEIT
                     IYNLLGQKVRTLRNETIPPGEHVVSWDGKDELGRRVPAGIYFYRMTASGFSKTRKMIL
                     IR"
     gene            339..2774
                     /locus_tag="AC16GM_07605"
     CDS             339..2774
                     /locus_tag="AC16GM_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923576.1"
                     /translation="MLRRWLLTVCFPVGFVAGAQAMAVTLSGNVTNPNGVGVAGVVVD
                     LYDPVTGDKIPIQKNTTDSLGNYNLNNPIGVPAATYNVAFKPLLASGVVPVIIRNYAL
                     SGNQVLNDTLPFGFVLSSFVRDTAGVGLPDIDLKVTSDSTGAAVYTPGATDPNGFYAT
                     RIPAGVFTLVYRPIAGQKLASVQYKGTVSHVDTTRNVTLRAGFFASGTVLDNLSTPVG
                     GANIKFHVSATQELVSTLNHTTDIAGHYQVTVAPGTYDISVEPLLANKIVGGKKFAVT
                     ITRDTTINFTLPHGQYLSGFARRANNLAVVPNVSIDVRDTLTSAKLVTPFDITDSTGF
                     YKIVVPAGTFNVDFLPPLATGLASVESARVAVAADRALNATLPAGVTLSGSLQRTGGG
                     GLFNVEVKFHNPSTDAKIPLANNFTDALGNYAVAAVPGTYNVEFNPPKAARRVAQEFL
                     NFALNANTTLNVTLDSGRSISGVVKDSLNNPLRDVDFNAFTLPARSKVFTPSDNTDST
                     GFYQVIVPAANLDLAYTPPLLSRFAGVMFAGLPITKDTTINLILRHGVELSGTVRDSA
                     GARVAGVEVHAFGSPETPLANGATDSAGFFAGILVPGTYSLLFVPPPASGLDSLRLTN
                     VVVQRDTTIGVTLLHPGAQTCCLITPNAIDFGQVPVGQSKDTTFTVANCGTTTQTGTI
                     SENCVEVSLIAGGGAYSLASGQSRTVTVRYAPLPNSPGVLTCAVRTGNLACPVVNLTG
                     AIAGCVLRGDVTNDAVADITDIVALIQNVVFDIGLPNPQAGDTNCDSVRDISDIVLLI
                     QYVVFGNPTPCCL"
     gene            complement(2962..3192)
                     /locus_tag="AC16GM_07610"
     CDS             complement(2962..3192)
                     /locus_tag="AC16GM_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2923577.1"
                     /translation="MPAIFRFLGYDPYPAEPSQPLSARLKACRRQLGLSQERLAGLLK
                     VDESTIAHWEQGRNRPSRRLLQAIDAFLASQP"
     gene            complement(3168..3395)
                     /locus_tag="AC16GM_07615"
     CDS             complement(3168..3395)
                     /locus_tag="AC16GM_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR2923578.1"
                     /translation="MPFCYAKLCAPKPLPKAYPKSLSSIGDHIRKRRLDLGLWQEQVA
                     RLIGVTESTVTNWEMQRNTLRYGSCQRFSGS"
BASE COUNT          701 a         1049 c          937 g          756 t
ORIGIN      
        1 ctcattctcc cgggcctggt cttccgcccc ctgccggaga aattcaccct gggaaccaat
       61 ttccccaatc ccttcggatc ccaaacgcaa ctcccattcg cggtgcagga ggagtcgcgc
      121 gtggagataa ccatatacaa tctcctgggc caaaaagtac gcacgctgag aaatgagact
      181 ataccaccag gggaacacgt cgtatcatgg gatgggaaag atgaattggg ccggcgggtg
      241 ccggccggaa tttactttta ccggatgacc gcctctggtt tttctaagac gcgcaaaatg
      301 attctcattc gttaacggac aactttttcg gagggagtat gctgcgccgc tggcttttga
      361 ccgtctgttt tcccgtggga tttgttgccg gagcccaggc catggccgtt accctttccg
      421 gcaacgtcac caatccgaat ggggtcggag ttgccggcgt ggtcgttgat ttatacgatc
      481 cggtcaccgg cgacaagatt cccattcaaa agaacaccac cgactctctg ggaaattaca
      541 acttaaacaa tcccatcggt gtgccggccg caacttacaa tgtggccttc aaaccgttac
      601 tcgccagcgg cgtggtgccg gtcattatcc ggaactacgc cctttccggc aatcaggtct
      661 tgaacgacac gctccccttc ggctttgtcc tttcgagctt tgtgcgcgac acggccgggg
      721 tagggctgcc ggacatcgac ttaaaagtaa cctcggacag caccggcgcc gcagtttaca
      781 ctcccggtgc gaccgatccg aatggttttt acgctacccg catcccggcc ggagttttca
      841 ctctggttta ccgtcccata gctggccaga aactggcctc ggtccagtac aaaggaacgg
      901 tcagccacgt cgacacgacc cgcaacgtaa ccctccgggc cggtttcttt gcctccggca
      961 cggtcctgga taacctgtcc actccggtcg gtggggccaa tattaagttt cacgtttcgg
     1021 cgacccagga actggtctcg acactgaacc acaccaccga catcgccggc cactatcagg
     1081 tgacagtagc gcccggcacc tatgacatca gtgtagagcc cctgctggcc aacaagatag
     1141 ttggcggcaa gaaattcgcc gtgacgatta cccgagacac caccatcaat ttcacccttc
     1201 cgcacggtca atatctttcc ggttttgcgc gccgcgccaa caatctggcc gtggtgccca
     1261 acgtgagtat tgatgtgcgt gacaccctga ccagcgccaa gctggtaacg ccctttgaca
     1321 ttaccgacag caccggattt tacaagattg tggttccggc cgggacattc aacgtcgatt
     1381 tcctgccgcc cctcgcgacc ggcctggctt cggttgaaag cgcgcgggtc gccgtcgccg
     1441 ccgatcgggc cctgaacgcc accctgccgg cgggtgtcac gctctccggc tcccttcagc
     1501 ggaccggggg tggcggattg ttcaacgtgg aggtgaagtt tcacaatccc agtaccgacg
     1561 ccaagatacc tctggccaac aactttactg acgctctggg gaactacgct gtggcggccg
     1621 taccggggac gtataatgtg gaattcaatc cgcctaaagc tgcccgccgg gtggcccagg
     1681 aatttttgaa tttcgccttg aacgccaata cgaccttgaa tgtgacgctc gactccggcc
     1741 gttcgatttc cggggtagtc aaagattcct tgaacaatcc gctgcgggat gttgacttta
     1801 acgccttcac cctgccggct cgctccaaag tcttcacccc ctcggacaac accgattcca
     1861 ccgggttcta tcaggtgatt gtcccggctg ccaatctgga tctggcctat acaccgcccc
     1921 tgctttcccg tttcgctggg gtgatgtttg ccggacttcc gataacgaag gacacgacga
     1981 tcaacctgat tttgcgccac ggggtcgagc tttccggcac ggtccgggat tcagccggcg
     2041 ctcgcgttgc gggagtggaa gttcacgcat ttggctcgcc ggaaacgccc ttggccaacg
     2101 gcgcgaccga cagcgcggga ttcttcgccg ggattctcgt gcccggcacc tacagtctgc
     2161 tctttgtccc accccccgcc agtgggctgg actccctgcg gctgacgaac gtggtggtcc
     2221 aacgggacac cacgattggc gtgactctct tgcaccccgg cgcgcagacg tgttgcttga
     2281 ttactcccaa cgctatcgac ttcggccagg tgccagtagg acagagcaaa gatacgacct
     2341 tcaccgttgc caactgcggc acgaccacgc aaaccgggac gatcagcgag aactgcgtgg
     2401 aggtcagcct cattgcggga ggaggtgcct actccctggc gtcaggacaa tcccgcaccg
     2461 tcacggtgcg ctatgctccc ctccccaact cccctggggt gttgacctgc gcggtgagaa
     2521 ccggcaatct cgcttgtccc gttgttaact taaccggtgc tatagcgggc tgcgtgcttc
     2581 ggggggatgt gaccaacgat gccgtagccg atatcaccga catcgtggcc ctcatccaga
     2641 atgtggtgtt cgatattgga ctccccaatc cccaggccgg agacaccaac tgcgacagcg
     2701 tacgggacat ctcagatatt gtgctgctca tccagtatgt ggtattcggt aacccgacgc
     2761 cttgttgctt gtaagcgccc cgattcgatt cagaatttgg cgggtaactt gaaccggctg
     2821 tagagaccaa cccgcgcttg aaaaatcaaa aaagaaggaa acgataactg caaggggagt
     2881 cgcatgtttt ccggcttact ttgtcttaag tcaattaagc ccggacctga catagcccga
     2941 tgatacgtca gccgctgggc gttaaggctg ggaagccaga aaggcatcaa tagcttgcaa
     3001 caaccttctg ctaggccggt tccgcccctg ttcccagtgt gcgatcgtgc tctcgtccac
     3061 tttgagcaat ccggccagcc gctcctggct cagccccaac tgtctgcgac aggccttaag
     3121 ccgggctgac aagggctgac tgggttcggc tggataggga tcatagccta ggaaccggaa
     3181 aatcgctggc atgaaccgta gcgaagggtg ttgcgttgca tctcccagtt ggttacggta
     3241 ctctcagtca ccccgatcag ccgggcgacc tgctcctgcc aaagaccgag gtcaagccgt
     3301 ctttttcgga tgtggtctcc tatggaagac aatgacttag ggtaggcttt aggaagtggt
     3361 tttggagcgc aaagttttgc gtaacaaaat ggcaatgaac ccctgcaact gtggtttctt
     3421 tggcgatccc ggtctcgggc ggc
//
LOCUS       DBNGZD010000286         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_255901, whole genome shotgun sequence.
ACCESSION   DBNGZD010000286 DBNGZD010000000
VERSION     DBNGZD010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_255901"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="AC16GM_07620"
     rRNA            complement(<1..1081)
                     /locus_tag="AC16GM_07620"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1665..2378)
                     /locus_tag="AC16GM_07625"
     CDS             complement(1665..2378)
                     /locus_tag="AC16GM_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR2923579.1"
                     /translation="MISEQFDSPHHLWEYSHFEDAANKAQEAQVPTGRYSREEAFWEV
                     LKTNPEPLPTVVRKTSFNTRMYHLGQSRFFSYLLSVCLLILALPLFLLIGFLLFVESP
                     GPVIVRVPRLGEDRRRLNRRGAEGIPRWDKRFRDRRRENLLGRPFTAFRFRTVSRAPA
                     EYVQALHRHPDTTWVGWVLEGTGLDHLPQLLNVLKGDLVLIGPRPEPLEIIHDLPEHA
                     GRYPTLLRVKPGFFVAGPI"
BASE COUNT          555 a          793 c          667 g          594 t
ORIGIN      
        1 acgagctgac gacagccatg cagcacctgt gctggctccc cttacggggt cgttcgtctt
       61 tcgactccct accaccagca tgtcaagccc aggtaaggtt cttcgcgttg cgtcgaatta
      121 aaccacaaac tccaccgctt gtgcgggccc ccgtcaattc ctttgagttt caaccttgcg
      181 gccgtactcc ccaggcggag cacttactgc gttagctgcg gcactgaacc cggtcgacgg
      241 gtccaacacc aagtgctcat cgtttaccgc tgggactacc agggtatcta atcctgtttg
      301 ctccccaagc cttcgtcgct cagcgtcagg tacggaccag gaagccgcct tcgccaccgg
      361 tgttcctcct gatatctacg catttcaccg ctacaccagg aattccactt ccctcttccg
      421 tcctcaagaa cagcggtttc ggcggcggat tctccgttga gcggagagct ttcaccgccg
      481 acctgccgtc ccgcctaccg accctttacg cccagtgatt ccgaacaacg ctcgttcccc
      541 ccgtattacc gcggctgctg gcacggagtt ggccggaact tcctctggag gtaccgtcag
      601 tctcccgtaa gggagagatt cgtccctcct gacaggagtt tacatcccga aggacttcat
      661 cctccacgcg gcgttgctgc gtcagggttt cccccattgc gcaatattcc ctactgctgc
      721 ctcccgtagg agtctgggcc gtatctcagt cccagtgtgg ccggacaccc tctcaggtcg
      781 gctaccgatc gcaggcttgg tgggctgtta tcccgccaac tacctaatcg gccgcgggct
      841 cctcctgcga cgaccccgca ttgctgcggg acctttcttc cccgggtcag gcgacccaag
      901 gaacgtacgc ggtattagac ctcctttcga aggcttatcc cccgtcgcag ggcagattac
      961 ccacgtgtta cgcacccgtt tgccgctttc gggtcccctt gcggggacct tttcgctcga
     1021 cttgcatgta tgaggcacgc cgccagcgtt cgttctgagc caggatcaaa ctctccacaa
     1081 gtgaaaaatt atttcacgtg caaagagcgt ccaaaaacgc tcgtgttgtt ctgaatcgcc
     1141 ttaccgcaaa attccgtaag gcccacaaaa gacgtgctgg cttgtacacg tcacgcttta
     1201 tgaagttttc aaagaacaga cgttacggca gagacacccc tctgccctgc cgggtcgata
     1261 cttctgcccg gcgaacgtgt tagtatacga gcccttttca gtttgtcaag gggttttttc
     1321 ggttctttcc agaattcccc cttgacctcc accatctact gtcattgtcg gtaataagct
     1381 accgctttca aaattgattg tcaagcctct tttctgcctg accgacccat tgttgtgaaa
     1441 aactcaaaat caggtcagcc aggcgtgggg gtgcatcttc ccaatgggtc gaaccggccg
     1501 atgcgcccgc ctttcagggt tttgaggagt tctgattttc ggtctaacaa tacatcacag
     1561 ggtgaacgta aacgttcacc cctacaaaaa acttgcgcga ttcagcagaa taaatagggc
     1621 aggaaactta cttgcacgtc atcgcccgca gggtcgatct tgcgttaaat cgggcctgcg
     1681 acaaagaacc ccggcttgac gcgcagcagg gtggggtagc ggccggcgtg ttctggaagg
     1741 tcgtggatga tctcgagcgg ctccggccgc ggaccgatta agaccagatc gcctttgagg
     1801 acattcaaca actgcggcag atgatccaga cccgttcctt ccagaaccca gccgacccag
     1861 gtcgtgtccg gatgccggtg gagcgcttgg acgtactcgg ccggagcccg gctgaccgtg
     1921 cggaagcgga aggcggtgaa cggccgtccc agaagatttt cccgccgccg atcccggaag
     1981 cgcttatccc agcggggaat tccctccgcc ccgcggcggt tcaagcgcct gcggtcttct
     2041 cccaaacgcg gcacgcggac gataaccggc cccggcgact cgacgaagag caaaaagccg
     2101 atcaaaagaa aaagcggcaa ggccagaatc aaaaggcaaa ccgaaagaag gtacgagaag
     2161 aatcggctct gccccaggtg gtacatgcgg gtgttgaatg aagtcttcct gacaacggtg
     2221 ggcagcggtt cggggttggt cttcagcact tcccagaaag cttcctctcg tgaatatctc
     2281 ccggtgggaa cttgagcttc ctgagccttg ttggcggcat cctcaaagtg ggagtattcc
     2341 cacaggtggt gcggactatc aaactgctcg gatatcatct actcacgtcc tctcatactt
     2401 ttggcactat catatatccg caacgccttt gcccatcagg actcaaatct aactctttta
     2461 ttaatcaggg taacttatta aaggataatt agtaagcggt ggtcaaaatt tgaccactgc
     2521 ttctcagttc cggggagatt tgattcccga tgcaagaaac ttcagaatcg gaagcaatca
     2581 acgaaaaagg ttggccggct attgcaccc
//
LOCUS       DBNGZD010000287         9416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1083922, whole genome shotgun sequence.
ACCESSION   DBNGZD010000287 DBNGZD010000000
VERSION     DBNGZD010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1083922"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1356)
                     /locus_tag="AC16GM_07630"
     CDS             complement(55..1356)
                     /locus_tag="AC16GM_07630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR2923580.1"
                     /translation="MKASPTLNERAKVTDRVLSDRALAVPGSAIHLITQMIEELERKG
                     AKDIIRLHLGDPDFATPPKVIEATNQALASGKTHYTLARGLRPLREAVGKRLQEKYGL
                     AGDADLITMAAGSAQGLGVAFHAVAGYGDQILFPEIFWPNFFQQAALYGIKVGFYPLT
                     TDYEPDLDRLEACLTPETKAILINSPSNPTGAVFPEKTLRKMYDFACRHNLWVVSDDA
                     YDDIKFTGESFVIAQLERDLPEGQRRVFSIFSFSKSFAMTGFRMGYVHAPSLQAARIL
                     GNLQVPMTGALNTPAQYAALAALSEPALTKEMCAAYQRRRDLALNVLKKHGMYDYTPQ
                     GAFYLLVDISRTGLDGDAFAQKLLQEKKVAVAQGSGFGFMPQYDARGRIVRSVDEHGL
                     ARYPVNSKVAGKVRVSFCVAEEQVEEGMEKLCGFCQRISNG"
     gene            1504..3510
                     /locus_tag="AC16GM_07635"
     CDS             1504..3510
                     /locus_tag="AC16GM_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR2923581.1"
                     /translation="MPKPKRLITAEDLLKFKWIRSVALSPEEKQIAYTLEWIDETKKK
                     YWSNLWVVPATGGAPRPFTQGKVKDREPVWSPDGKLIAFFSVREEKDGIYVIPADGGE
                     ARQIVTLDGSFASLSWSPDGKKILCAFRKNDVPQDGEKKDEKEKKKEAPVFRHITRLF
                     YRLDGEGFLPKDRYHIWTFDAASGKGKQLTFGRFDEFGPAFSPDGRWIAFASNHQPNP
                     DRAFLRVDLWLVPASGGKLKKIPTPAGPVESPAWSPDGKKIAYLGHTNPEDAWGVTNY
                     HPWVVPAEGIGKAKDLTPDFDRTCIDETICDMQEGFALPAPFWSQDSKRLYFVASDFG
                     STRLFSVAAKGGKVTRILNRPGHLQKYSAAPKAGIAALALADFSSPAEIWTIRLKGRP
                     NRPRQVSKISREFLSDIRLSRPEAAGFRSFDGTPVQAWIMKPPRFKPGRKYPAIVEIH
                     GGPRAQYGNTFFHEFQLLAAKGYVVFFSNPRGSQGYGEGFAGAIINDWGNLDYKDVMA
                     GVDTLVKKPFVDPKRIGVTGGSYGGYLTNWIVGHTNRFKAAVTQRSISNFYSFVGSSD
                     IGFEDHRELGAQPWTNPEELMRMSPITYVKNIRTPLLILHNENDLRCAVEQAEQFFTA
                     LKFLKRKVEFVRFPEEPHGLSRHGRPDRRLARLSWILRWFGRYL"
     gene            4030..5385
                     /locus_tag="AC16GM_07640"
     CDS             4030..5385
                     /locus_tag="AC16GM_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR2923582.1"
                     /translation="MSSTQTQCQYKVLGDSDYKCPYPVERDAYCIFHMHTRYGDDFKK
                     FNDGISTLLENQTGGAYDFRGFRFPSINFSGKVFEKPLLLDQAVFNCRLEFHKSRFSQ
                     KVDLRHAAINDWFVLSDVEFNDGVDMLGTVCREPARFFHVKFHNASFFKTTFQSVLFE
                     DSHFTGMTLFNKTVFLEPADFISSTFQLVYFVETEFDQRVLFQEGDFFKHAEFTGAIF
                     NCEAIFKGTIGKKVFRDDAIFGSLKIGKEAHITFEKINLSKASFLDTNLERITFRDVE
                     WTSRKKFWGCSGKALWDESRPLEQDEERDYEKIAENYRQLVLNHERKRDYDAAEDFHI
                     GEMAMRRKKKGASIKWLWLRPVREQMNAYNLYRLLSYYGTSYWQAVFGLLAFLFLLPF
                     GYCSTDYWQGFLRPLSILTFQRPAEQLDRWSRFWLSISVIVLTAQVALVLLAIRRRFK
                     R"
     gene            complement(5700..6206)
                     /locus_tag="AC16GM_07645"
     CDS             complement(5700..6206)
                     /locus_tag="AC16GM_07645"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR2923583.1"
                     /translation="MPQLESEELVADIQVTKPQGRPLIRLFASGFGMGYLFPFPGTWG
                     SLPPVFLAWYLLSRFPTWYLVLLPLFLIAGAYVATRAERLWVHDDRRIVVDEFVGMLV
                     SLFLVPRSFGYYLAGFLLFRFLDIFKPFPARRAENLPEGWGVMADDVIVGIYANLLLQ
                     LYSLLAGR"
     gene            complement(6280..6867)
                     /gene="pgsA"
                     /locus_tag="AC16GM_07650"
     CDS             complement(6280..6867)
                     /gene="pgsA"
                     /locus_tag="AC16GM_07650"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR2923584.1"
                     /translation="MNLPNQLTLARIILSPVFMIFFLIDNLYSRLFSLFVFVAAALTD
                     LYDGYLARKTGVTTGFGKFMDPLADKILTSTAFICFVALGYASTWMVLAIVVREFLIT
                     GLRSLAAYKGVVIVPSLLAQVKTVLQMSVIVVILVYINVKTILVSNGHPWAIFSSPWT
                     PRAFNGLIFLTTVVTVGTGIDYLVKSAPLLRGVLK"
     gene            complement(7337..8236)
                     /locus_tag="AC16GM_07655"
     CDS             complement(7337..8236)
                     /locus_tag="AC16GM_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR2923585.1"
                     /translation="MKIFNSILDTIGNTPLVKIQKLNPNPNVLLLAKLEWFNPMGSVK
                     ERIALAMVEAAELDGTLTKDKIILESSSGNTGIGLAMVGAVKRYKVAIVMSEGVSVER
                     RQIMEALGAEVILTSKEGGSDEAWAKADELAEKFPKKYCRLSQYRHPANVRTHYARTA
                     QEIWEDTEGKVDVVVGTLGTTGTIVGVSRRLKELNPKIRIVAVEPQPGKHKQQGIRNI
                     STSRVPDIWDDRAVDARKVIKDADAFRMTKELARQEGLFGGISSGTAMYAAVAEARKL
                     PAGIIVVILPDSGFKYLSTELFQ"
     gene            complement(8335..9327)
                     /locus_tag="AC16GM_07660"
     CDS             complement(8335..9327)
                     /locus_tag="AC16GM_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923586.1"
                     /translation="MRKLLIAGIAASSFFLIAGKSDIEESKRTVFSLLMVNNLKGEFS
                     FELDPACKYSNGKFEDVSVQLDSAQPPSGFIFNKVKDFTIFKRGDEIGKFNVAKIERG
                     GLNCYGFWAGVGNCTISDSLLKALGSEHSNAENYTDQPVFLTALNSSSGAVKTQIHSA
                     PLKTTLSEIQKKKLLEIARQKYQDQKVDQKFLKNMKIEKSISYDLDGDGTIEHILKTS
                     IETIDTEYAEAIETGNSTDSTPYIHEDTKIMLIWARIDGQNVTTIPISAKEGKFDVWE
                     NAYDLVDVLDIDGDTIPEVVFVAEIAQIEYAGFQIYQFINGKFQLVYFGAGSGC"
BASE COUNT         2273 a         2427 c         2543 g         2173 t
ORIGIN      
        1 ctgtacaaat ttagacattc gatttggcag atggtctgtc tatataatcc gtattcatcc
       61 gttcgaaatc cgttgacaga aaccacatag cttttccatc ccctcttcca cctgctcctc
      121 cgccacacag aaggagaccc gcaccttgcc ggccactttg gagttcaccg gatagcgggc
      181 caggccgtgt tcatcaaccg aacggacaat gcggccgcgg gcgtcgtact ggggcataaa
      241 gccgaatccg gaaccctgcg ccaccgccac tttcttttcc tgcagaagtt tttgggcaaa
      301 ggcatcgccg tccaggccgg tgcgggaaat atccacgagc aagtagaacg ccccttgagg
      361 ggtgtaatcg tacatcccgt gctttttgag tacgttcaac gcgagatcgc ggcggcgctg
      421 gtaggcagcg cacatttcct tcgtcagcgc cggttcagag agggccgcca gggcagcgta
      481 ctgggccggc gtattgaggg cgccggtcat tggaacttgc aggttgccca ggatccgagc
      541 cgcttgaagg gagggagcgt gaacataccc catccggaag ccggtcatgg caaagctctt
      601 ggagaaagag aagatggaaa aaacccgacg ctggccttcg ggcaagtctc tttctaattg
      661 ggcaatgacg aatgattcgc cggtgaactt gatgtcatcg taggcatcat cgctcaccac
      721 ccagaggttg tggcgacagg cgaagtcata cattttccgc agcgtttttt ccggaaaaac
      781 tgcgccggtg ggattggagg gggaattgat gagaattgcc ttggtctccg gggtcaaaca
      841 ggcttcaagg cgatcaagat cgggctcgta gtcagtggtc aggggataga atccgacttt
      901 aataccgtac agagccgcct gctggaagaa gttgggccag aaaatttcgg gaaagagaat
      961 ttgatcgcca tacccggcaa cggcatggaa agccacaccc agcccctgcg ccgagccggc
     1021 ggccatggtg atgaggtcgg cgtcgccagc caggccgtat ttttcctgta gtctttttcc
     1081 aactgcttcc cgcagcgggc gaagcccgcg ggccagggtg tagtgcgttt tgccggaggc
     1141 gagggcctgg ttggtggctt caatgacctt tggcggagta gcgaagtcgg gatcgcccaa
     1201 gtgcagccgg attatgtctt tcgcgccttt tcgctccaac tcctcaatca tctgggtgat
     1261 gaggtggatg gccgagccgg ggaccgccag ggcgcggtcg gacagaaccc gatcggtgac
     1321 tttggctcgc tcgttcaaag tgggtgacgc tttcatcggt ggatttcttt ccctaactct
     1381 ttactgaaag ttctgcgcaa attatcgccc cgagggccga aacgcaagtt cggcttgtgt
     1441 caaacgccat tttgatgcaa attgagcccc tcgtccgtga acgaaacctg aattgaggag
     1501 tgtatgccaa agccgaaacg tctgatcacc gccgaagatt tgctcaaatt caagtggatc
     1561 cgctcggtgg cgctttcgcc tgaggagaag cagatcgcct acaccttaga gtggatcgac
     1621 gagacgaaaa agaaatactg gtcgaatctc tgggtggtcc ccgcaaccgg cggcgcgccg
     1681 cggcccttta cgcagggaaa agtcaaagac cgggagccgg tctggtcgcc ggacgggaag
     1741 ctcatcgcct tcttctccgt gcgcgaggaa aaagacggca tttacgtcat ccccgccgac
     1801 ggcggggagg cgcgtcaaat agtcacgctg gacgggtcgt tcgcttcgct ctcctggtcg
     1861 ccggacggaa aaaaaatcct ttgtgccttc cgcaaaaatg atgtgcctca agatggcgaa
     1921 aaaaaagatg aaaaagaaaa gaagaaggaa gcgccggttt tcaggcacat cacccggctt
     1981 ttttaccggc tcgacggcga aggttttttg cccaaggacc gctatcacat ctggacattt
     2041 gacgccgctt ccgggaaggg aaaacagttg accttcggcc gttttgacga gttcggaccg
     2101 gccttctcgc ccgacggccg ctggatcgct tttgcctcca atcaccaacc caatccggac
     2161 cgcgccttcc tgcgggttga cctgtggctc gttcccgcaa gtggaggaaa actgaagaaa
     2221 attcccacgc ccgccgggcc ggtcgagagc cccgcctggt caccggacgg gaagaagatc
     2281 gcttacctgg gccacaccaa tccggaggat gcctgggggg tgaccaacta ccacccctgg
     2341 gtggttcccg cggaaggcat tggcaaagcg aaagatttga cacccgactt tgaccgaacc
     2401 tgcatcgatg aaaccatctg cgacatgcaa gaggggttcg ccctgccggc gcccttctgg
     2461 tcccaggata gcaagcgcct ctatttcgtt gcgtcagatt tcgggagcac gcggctattt
     2521 tcggtggcgg ccaaaggcgg caaggtcacg cgcatcctca atcgtccggg ccacctgcag
     2581 aaatacagcg ccgcgcccaa agccggaata gctgccttgg cgctggccga tttttcttcc
     2641 cctgctgaaa tctggacgat caggttgaaa ggccggccca atcggccccg gcaggtctca
     2701 aagatcagcc gcgaattctt aagcgatatc cgcctttccc ggcctgaggc ggccggtttc
     2761 agaagtttcg acggcacgcc ggtgcaggcc tggattatga agccgccgcg cttcaaaccg
     2821 gggcggaagt atccggccat agtcgaaatc cacggcgggc cgcgggcgca gtacggcaat
     2881 accttctttc acgaatttca actcctcgcc gccaaaggtt acgtcgtctt cttttccaac
     2941 ccgcgcggga gccagggata cggcgagggg ttcgccgggg cgatcatcaa cgactggggc
     3001 aacctcgact acaaagacgt gatggcggga gtcgatacgc tcgtcaaaaa accgttcgtc
     3061 gatcccaagc gtatcggcgt gaccggcgga agttacggcg gctacctgac caactggatt
     3121 gtggggcata cgaaccgttt caaagcggcg gtgacccagc gctcgatttc gaatttctac
     3181 agtttcgtcg gctcctcgga catcgggttt gaagaccacc gcgaattggg ggcccagccc
     3241 tggaccaatc ccgaggaatt gatgcggatg tcgcccatca cctacgtcaa gaatatccgg
     3301 acgccgctat tgatcctgca caacgagaac gacctgcgct gcgcggttga gcaggcggag
     3361 cagttcttca ccgcgctcaa gtttttgaag cgcaaagtgg agttcgtccg ctttccggag
     3421 gaaccgcacg gcctctcccg ccacggccgg cccgaccggc ggctggcgcg gctctcgtgg
     3481 atactgcggt ggtttgggcg atacctctaa aagccatatt ccaccacaga ggcacagtcg
     3541 gagactcgac tcttcgagag gacacggaga agacaagagt tccgggaaat gggcggggtt
     3601 tcaatctttt atctttctct aacgcagaca agggcttacg acaaaaaagc tgaacacggt
     3661 attcggcaca caggaaaagg caaaaaacca cctcttctct ggattcgtga gtgaggcgtg
     3721 cccttctatt atttggcaca ttgtccgttt tacccccgga acgaccccaa ataaaacagt
     3781 gatttgtgat gagtgacaag ggattagcga aaacgttgat cggagctaaa gccccgccta
     3841 cgcggaggaa ggcggtagaa ttggaccggc tcaggtcggt acgactctat cgaggacgag
     3901 ccggtctacc tagaggtggg gagagaattc acagcagatg gagcaaagcc atgaaaatga
     3961 aaccaagtaa gaaaagtaag gataacgact gagggaagtg caggtcttta gtgtatttta
     4021 tcactcacta tgagcagtac acagacgcag tgccaataca aagtgctagg tgattcggat
     4081 tacaaatgtc cctaccctgt tgaaagggat gcctattgta tctttcatat gcatacccga
     4141 tacggtgatg attttaagaa attcaatgat gggatttcta ccttgttgga aaatcaaacc
     4201 ggaggagcat acgattttag aggttttcgc tttccctcaa tcaatttcag cggaaaagta
     4261 tttgaaaaac ccctcttact tgaccaagca gttttcaact gtcgcttaga gtttcataag
     4321 tcgcgatttt cccaaaaggt tgacttgcgt catgcggcca taaacgactg gtttgttttg
     4381 agtgatgtcg aattcaacga cggggtcgat atgctcggaa ctgtttgtcg agaaccggcg
     4441 cgttttttcc acgtcaagtt tcacaacgcc agctttttca aaaccacttt ccaatcagta
     4501 ctctttgaag attcgcactt tacagggatg actctcttta acaaaacagt tttccttgaa
     4561 cctgctgatt tcataagctc taccttccag ttggtttatt ttgtggaaac cgaatttgat
     4621 caaagagttc tctttcaaga gggggatttc ttcaaacacg ctgaattcac aggagccata
     4681 tttaattgcg aggcaatctt taaagggact atcgggaaaa aagttttcag ggacgatgct
     4741 attttcgggt ctcttaaaat aggcaaagag gcacatatca cttttgaaaa aataaatctt
     4801 agtaaagcaa gctttctaga tacgaacctg gagcgaataa ccttccgtga cgtggagtgg
     4861 acctcgagaa agaaattttg ggggtgctca ggaaaagcac tgtgggatga gtctcgacct
     4921 ttggaacagg acgaagagcg cgattatgaa aaaatcgcgg aaaactaccg acaattagtg
     4981 ctaaaccatg aacgtaagcg agattacgac gccgcagagg atttccacat aggcgaaatg
     5041 gcgatgcgga ggaaaaagaa aggggcgagc atcaaatggc tttggttgcg acccgttcga
     5101 gaacagatga acgcctacaa tctctaccgg ctactgagtt actacggcac gagttactgg
     5161 caggcggttt ttgggcttct cgcatttcta ttcctcttgc cgtttggtta ctgttctacc
     5221 gactactggc agggattttt gcgcccgcta tctattctca cctttcagcg acccgccgaa
     5281 caacttgacc gatggtcgcg cttctggctg tccatctcgg ttatcgtttt aaccgcacag
     5341 gtggcgctgg tgcttttggc gatacggcgg cggtttaagc ggtgagctga cctcacctca
     5401 ccctaccctc tcctacagga gagggaaacg agcggaacgg tagacccctc cctcgccctc
     5461 ccccgcctct ggcgggatct ggcgaccctc aaggggaggg aaaggttcac ctcagattct
     5521 ggaagtcatg cgggatagga aaaccatccc gcactacggg gtaattttac tttcagaacg
     5581 gagataagca cgtagtgcgg gttggtcttg taacctgcgc gtacgggatt ggaaaaccat
     5641 ttcggtacga gtccatacgg atccgtaggg atcgtgccga cccgcactac ggggtaatat
     5701 caccgtcctg ccaacagact gtacaattgc aaaagtagat tggcgtaaat ccccacaatc
     5761 acatcatccg ccatgacacc ccagccctcg ggcagatttt cggcgcggcg ggcggggaag
     5821 ggcttgaaaa tatccagaaa gcggaaaagg agaaaaccgg cgagatagta gccgaaactt
     5881 cgaggaacta aaaagaggga cacgagcatc ccgacgaatt catcgaccac aatccggcgg
     5941 tcatcatgca cccagaggcg ttcagcccgg gtggccacgt aggcgccggc aattaaaaag
     6001 agcggcagaa ggaccaagta ccacgtcggg aaccgggata agagatacca ggcgagaaaa
     6061 accggcggca gggagcccca ggtgcccggg aaaggaaaga ggtagcccat cccgaagccg
     6121 gaggcaaaaa gtctgatcaa aggccttccc tggggcttgg tcacctgaat gtcggccact
     6181 aattcttccg attcgagttg cggcataatt tttctttcag caagagccac cgggggccga
     6241 ggacggaatc ctcggctaca gaaaatcgcc gggtttttat cactttagca ctccgcgcag
     6301 aagcggggcg gatttcacca gatagtcgat gccggtgccg acggtcacca ccgtggtcag
     6361 gaaaatcaaa ccgttgaagg cgcgcggggt ccaggggctg gagaagatgg cccaggggtg
     6421 gccgttggag acgagaatcg tcttaacgtt gatgtacacg aggatgacga caatgacgct
     6481 catctggagc accgttttca cctgcgccag aagcgagggg acgatcacca cccccttgta
     6541 ggcggccaga gacctgaggc cggtgatcaa aaactcccgc accacgatgg ccaggaccat
     6601 ccaggtcgaa gcgtagccca gggcgacgaa acagatgaag gcggtggagg tgagaatttt
     6661 gtccgccagg gggtccatga atttgccgaa accggtggtg acgccggtct tgcgggctaa
     6721 ataaccgtcg tacaggtcgg tcaaggccgc cgccacaaag acgaaaaggg aaaagaggcg
     6781 ggagtagagg ttgtcgatca ggaaaaaaat catgaaaacc ggtgagagga tgatgcgtgc
     6841 caaggtcaat tgattgggga ggttcatgtc cgtacccggc aggtaatcta cagcccctat
     6901 tgggggcaag tcaaccctgc tggcggagcg gttctgcaaa aagtcaaggg ctcctcaccc
     6961 cacgcgagaa gcccttgcca cctggtgtcc gtttttgcgt gcgttttgct taagctcgaa
     7021 agacgctggg ccccatcacc tcctcaataa tacattaggt cgccgtcgac aatggtaaac
     7081 aaaccgtgtg ccaccgtccc ttccacgtta tagaattaaa tctgttgtat cacatatagt
     7141 tatacaaaag gaaaatgctc cggtacgagc gcttttgact caggaacgca aaaagtgcgg
     7201 gttgtgaaag cggtcaagtc gaatttttta cgtttcgctc gttgccatcg gcagttaccg
     7261 atgcgcagaa tctgcggtta cagacctgtg gaggatgagt atttgacggt gaaacggacc
     7321 ttaacgaggc gattgattat tgaaacaatt cggtggagag gtatttaaag ccgctgtcgg
     7381 gcaaaatcac gacaataatc ccggcgggca gtttccgggc ttcggccact gcagcgtaca
     7441 tggcagtccc tgaactgatt cccccaaata gcccttcctg gcgtgccaat tccttggtca
     7501 tccgaaaagc gtccgcatct ttgatgacct ttcgtgcatc caccgctcta tcgtcccaga
     7561 tgtccggcac acgggaggta ctgatgttgc gtatcccctg ctgtttgtgc tttccgggct
     7621 ggggttctac agccacgatg cggattttcg ggttcaattc tttaagccgc cgtgaaaccc
     7681 caacgatggt tccggtggtt cccagcgtac ccaccaccac atcgactttg ccctcggtat
     7741 cttcccagat ttcctgcgcg gtgcgtgcgt agtgcgtccg gacattggcg ggatggcggt
     7801 actgggagag gcggcagtac tttttcggga acttttcggc caattcatcg gctttggccc
     7861 aggcctcgtc ggagccgcct tcttttgagg ttaaaatcac ttcagccccc agcgcttcca
     7921 ttatctgccg gcgctctacg gacaccccct ccgacatcac aatcgccacc ttataccgtt
     7981 tcaccgctcc caccatcgcc aggccgatgc cggtgttgcc gctggaggac tcgaggatga
     8041 ttttgtcttt cgtaagcgtt ccgtcgagct ccgccgcctc caccatcgcc agggcaatgc
     8101 gctccttgac tgaacccatg gggttgaacc attccagttt ggcgaggagg agaacgttcg
     8161 ggttggggtt caatttttgg attttcacca aaggcgtgtt gccgatggta tcgagaatcg
     8221 aattaaaaat cttcatactt cattgtatta acgccgatgc agaaattttg tcaacaagga
     8281 tcggaggaaa tgaggggatt ttgtgacttt agattgaggg agattaattt ttcattagca
     8341 accggagcca gcgccaaaat atactaattg aaacttaccg ttaataaatt ggtatatctg
     8401 aaaaccggca tactcaattt gtgcaatttc tgctacaaaa acgacctcgg ggatggtgtc
     8461 gccatctata tccagaacat cgacaagatc atacgcgttt tcccaaacat cgaattttcc
     8521 ctcttttgct gaaataggga tagttgtgac attttgtccg tcaattcttg cccagataag
     8581 cattattttc gtgtcctcgt gtatgtacgg cgtgctatcg gtggaatttc ccgtctcaat
     8641 agcttctgca tattccgtgt ctatagtttc aatgctagtt tttagaatgt gttcaattgt
     8701 accatcacca tccagatcgt aagatataga tttttcaatt ttcatgtttt tcaagaactt
     8761 ttggtcgact ttttggtctt gatatttctg tcgtgcgatt tccaaaagtt ttttcttttg
     8821 aatttctgaa agagtggtct tgaggggagc ggaatgaatt tgggttttga ccgctcccga
     8881 gcttgaattt agagcagtga gaaataccgg ttggtcagta tagttttcag catttgaatg
     8941 ctcagaaccg agggctttca atagtgaatc agaaatggta caattcccga cccccgccca
     9001 aaacccgtaa caattgaggc cgcctctctc aatttttgcg acattgaatt ttccaatttc
     9061 gtccccgcgt ttgaaaatag tgaagtcttt gactttgttg aagataaatc ccgaaggagg
     9121 ttgagcggaa tcaagttgaa cagaaacatc ttcaaatttt ccgttcgagt atttacaggc
     9181 cggatcaagc tcaaagctaa attcaccctt aagattattt accattaaca gggaaaaaac
     9241 tgtacgcttg ctttcctcga tgtctgactt cccggcgatg aggaaaaaag acgatgccgc
     9301 tattcccgct attagtaatt ttctcattct gcccatcacc tcttgtagag tatcggcttt
     9361 aacggaggtt gtattacccg agttcttcaa ttctatgtat caccagcccc gtgagc
//
LOCUS       DBNGZD010000288         7369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1436618, whole genome shotgun sequence.
ACCESSION   DBNGZD010000288 DBNGZD010000000
VERSION     DBNGZD010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1436618"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..2193
                     /locus_tag="AC16GM_07665"
     CDS             73..2193
                     /locus_tag="AC16GM_07665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2923587.1"
                     /translation="MRICLLAPATSIHTQRWARALAERGYEVRILSLHRAAEPLLDLD
                     VEYLPRPTGTKVDYFLALPVVRRRVERWRPDIVHAHYATSYGLLGVWVRFHPWVLSVW
                     GSDLLDFPKFSPLHRALVRYILEKPDQILATSPILAQAVRDLARTAPAVEIIPFGVDI
                     EKFTPRPNRSVTNGVTIGVVKTLEPKYGIEYLIRAFAILRARFPNVSLVVVGGGRLRR
                     HLEELSRSLGVPNHITFSGSVPHERVVDYLHTFDIFVVPSVLDSETFGVAAVEAAACG
                     LPVVASNVGGLPGVVRDGVTGFLVPPRNPQALADTLERLIRNPNLRTRMGQAGRDMVL
                     EQYRWEKCVDRQEEVYARYAPAKVGNSPWAKLSESTVLGIPDALPLVSIIVPVRNEQA
                     SIGGCLISLLGQDYPRDLLEILVVDGMSMDGTRDTVHQFMCCETRVRLLENPQKIVPT
                     AMNIGIRQARGTVVMRADAHTLFPKNYLRQCIVWLEQTGAACVGGVTQASPGARGLLP
                     QALALSLSTPFGVGNARFRLGAKRPTEVDAVAFGCHPRSVFERVGLFDERLLRNQDIE
                     FFHRLRLLGGKIFLLPDLQLTYLARSSLSLFLLQSFRNGYWNVLTERLKPGTMAWRHF
                     IPLLFVAGILVGLAFSWTLGGRLGLFALLGLYSLAAGGSAFVVALREGFRCFWLLPWV
                     FLSLHLSYGAGSLWGLVRVSTRRL"
     gene            2171..3328
                     /locus_tag="AC16GM_07670"
     CDS             2171..3328
                     /locus_tag="AC16GM_07670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR2923588.1"
                     /translation="MSALDDFKSLLRTFLAGGQVFLFNRGRVAFYALLRALDLKPGEE
                     VILPNFTCVAVANAIHYAGATPVYCDIDPHTYTLTAAAVERKITPRTRVILAQNTFGL
                     SSDLDPLLALARHYRLQVIEDCAHGLGGQYKGQPNGTVADAAFFSLQWSKPISTGLGG
                     IAVTSVPEIAEKLAVAEKAYLAPRLFESLSLRLLVAGYDRFFSPRFFWRAQHAYRRLS
                     QWGVVPGSSTWGEVNGVVKPRNFEKRYPAILARRGNHALQHLVQTIAHRQRVADAYDE
                     ILRQIDRVTPVQPAYAIHAFLRYPLEVRNRSGFLAEAQKRYIEVGDWFRSPLHPVEGD
                     LRPWGYQWGCGPVGENVSRHIVNLPTHRHVSPNEIGRIGDFLMDMKKRDIV"
     gene            3401..4096
                     /locus_tag="AC16GM_07675"
     CDS             3401..4096
                     /locus_tag="AC16GM_07675"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2923589.1"
                     /translation="MARIKAMYARRDSDRTLGARYHPLAPGNLFLTQGLERVILLELR
                     RAGVLDLAKLRILEVGCGNGLRLRDFVNWGADPTRLVGVDITPERIAQARRLSPNITF
                     VCANADRLEFSNGSFDLVFQFSTFTSLLDYAMKRRVAEEMLRVLANDGCILWYDFRVR
                     GREREHSAPIGRREIRELFAEGTVRLRSMTLAPPLARRLAPYWWTGCLLLEKIPLLRT
                     HWFGVIRKKRPTA"
     gene            4093..5298
                     /locus_tag="AC16GM_07680"
     CDS             4093..5298
                     /locus_tag="AC16GM_07680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR2923590.1"
                     /translation="MTRSKIVSDKQPEARQIPFYQPSITEREIRAVVQTLRSGWLTTG
                     PKTKKFEKRFARYVGAKHAVALNSCTAGLHLALVASGVGPGDEVIVPTYTFAASAETV
                     IHAGAKPVLVDSESDTLNLSVAEVEKEISPRTKAVVAVDIAGLPCDYAALQPVVKKQG
                     IHLIDDAAHALGASYDGRMVGTLADLTAFSFYATKNLTTGEGGMVTTDNQELAEKISL
                     LSLHGMSRDAWKRYRTGGSWYYELLDAGFKYNFTDLQAALGLPQLARLRTLQAQRRAR
                     ATLYDRELGDLPEVRLPAQKKGRVHAWHLYIIRLRLEQLKIDRNQFIHELTRRRIGTA
                     VHFIPLHLHPYYRERYGYQPEDFPVAHDNYQRAITLPLYPRMTRAMVCYVAGAVREIV
                     QKYRHRGRG"
     gene            5295..>7369
                     /locus_tag="AC16GM_07685"
     CDS             5295..>7369
                     /locus_tag="AC16GM_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR2923591.1"
                     /translation="MSVTPPVIAKKLRNTVLLFLGDQAVLWGAFLFALWLKGLTRNLG
                     FFDYLEPGLLLAGLWVVIFLVSGIYWRGSRSFLHELLRINNAVALGILILLLATFDVT
                     SPLSQVRVTLLGYWAVLLAGLWLFRWLFWQKFNRQLVSGFPELPTPLVLLRRLGILGM
                     DLALAGLSLFLAFLLRFDGRIPANHLTIFFYLLPLVVIIRFSAFSLAGLYSGLNRYAS
                     IADLFTVLKGVSFGTALFVLPAAVQWLVNHLDPKVASTLGLTWLLPLFPRLHGYPRSV
                     FFIDWLLMVIFIGGSRFAIRALRELAPNFLREGKRTLIVGAGNAGEMLMREIRRNRSL
                     PYLPVGFIDDDPNKRGLRVHGLPVVGSHREIPQLVSRLGAEQIVIAIPSATGIQMRRI
                     VEACQQSKVEIQTLPPLKEILGGQVDLHQVRKVRLEDLLGREPVQIETGQIASFLEGR
                     RVLITGAAGSIGAELVRQVLRFRPLQVLAVDRAENNVFALEQEIAARTDRSRAILSIG
                     DVTDGIRMDDLFASFRPEVVFHAAAYKHVPLMEDHPDEAVKNNVAGTIGMADLAERYG
                     TRKFVFISTDKAVHPTSVMGATKRVAELYLQAKPAAPTAFVSVRFGNVLGSDGSVVPL
                     FQKQIARGGPVTVTHPEVSRFFMTTSEAVQLVLQAASIGQAGSVLLLDMGEPIKILDL
                     ACDLITLSG"
BASE COUNT         1371 a         2090 c         2208 g         1700 t
ORIGIN      
        1 ttcattctac caccgagaaa aacacgagtc gggttgatct acagccgcag ggccggtgaa
       61 caaaggacgt taatgcgtat ttgtttgctt gctccggcca ccagcatcca cacgcagcgt
      121 tgggcgcggg cgctggccga acggggttac gaagtccgca tcctctccct tcatagggca
      181 gcagagccgc tcctcgatct cgacgtggag tacttgccgc gacctacggg caccaaagtc
      241 gattattttc tggcactccc tgttgttcga aggcgagtag agcgttggag gcccgatatc
      301 gttcatgccc actatgctac cagttacgga ttgttaggag tctgggtgcg tttccatccg
      361 tgggtgctgt cggtctgggg ttcagacctt ctcgactttc ctaaattctc accattgcac
      421 agagctctgg tacgttatat cctggagaaa ccggatcaga ttcttgcgac cagtcccatt
      481 ttggctcagg ccgtccggga ccttgcgcgg acggcgccgg ctgtcgagat tattcctttt
      541 ggggtcgaca tagaaaagtt cacaccgcga ccgaatcgga gtgtcaccaa tggcgtaacg
      601 atcggggtgg tgaaaacgct ggagccgaaa tatggcattg agtacctgat tcgggcgttt
      661 gccatcctcc gcgccaggtt ccccaatgtc tccctggtcg tcgtcggcgg gggaaggttg
      721 cggcggcacc ttgaagaact cagccggtcg cttggtgtgc cgaatcatat taccttctcc
      781 ggatcggtgc cccatgagcg ggtggttgac tacctgcaca ccttcgatat tttcgtcgta
      841 ccatcagtgc tcgattccga aacttttggt gttgccgcag ttgaagcggc ggcctgcggg
      901 ttgccagtgg tggcgagcaa cgtgggcgga ctgcccgggg tcgtacgaga cggggtgacg
      961 gggtttctgg tgcctccccg aaatccgcaa gctttggctg acacactgga acggttgatt
     1021 cgtaatccca atctgcgcac gaggatggga caagccgggc gtgatatggt gctggagcag
     1081 tatcgctggg aaaagtgcgt ggatcgacag gaagaagtct acgcacgtta cgcaccggca
     1141 aaggtaggta actccccttg ggcaaaactg tccgaaagta ccgtcttagg aattcccgac
     1201 gccttgccgc tggtatcaat catcgtgccg gtaaggaatg agcaagcgtc tattggtggc
     1261 tgtctgattt cgcttttggg acaagactat ccccgagatt tgctggaaat tctcgtcgtg
     1321 gatgggatgt cgatggatgg tacccgggat actgtccatc agttcatgtg ctgtgagacg
     1381 cgggtgcgtt tattggaaaa tccacagaaa attgtcccca ccgctatgaa catcggtatc
     1441 cggcaggccc ggggtacggt ggtgatgcgt gccgatgcgc acaccctttt ccccaagaac
     1501 tacctcaggc aatgcattgt ctggctggaa caaaccggtg ccgcctgcgt gggaggagtc
     1561 actcaggcga gcccgggggc gcggggtctt ctgccccaag cactggcact ttcgctctcc
     1621 acaccttttg gggtgggcaa tgccaggttc cgcttgggag cgaagcggcc gaccgaggtc
     1681 gatgcggtgg cgttcggatg ccacccgcgg agcgtgttcg aacgagtcgg tttgttcgat
     1741 gagcgtctgc tgcggaacca agacattgag tttttccatc ggctgcgtct tttgggcggg
     1801 aaaatcttct tgctaccgga cctgcagctc acctatctgg cacgctcgtc gctttctctg
     1861 tttctcctgc agagcttccg caatgggtac tggaatgtgc tcaccgagcg tctcaagccg
     1921 gggacgatgg cctggcgaca tttcattccc ctgctgtttg ttgcaggaat actggtcggc
     1981 ctggcgttct cgtggacact cggaggacga ttaggactgt ttgcgcttct gggactctac
     2041 agcttggcgg ctggtggcag tgcctttgtc gttgctctga gagagggttt tcgctgcttt
     2101 tggcttctgc cgtgggtttt tttgtccctc catcttagtt acggtgccgg atcactttgg
     2161 ggattagtgc gtgtcagcac tcgacgactt taagtctctg ctccggacct tcctggctgg
     2221 cggacaagtt tttttgttca accgaggccg ggtggccttt tacgctttgc tccgtgcctt
     2281 agaccttaag ccgggagagg aagtcatcct gccgaacttc acctgtgtgg cagtggccaa
     2341 tgcgattcac tacgccgggg caacaccagt ctactgtgac attgacccgc acacgtatac
     2401 gctcaccgct gcagcagttg agcggaaaat tacgccgcgg acgcgcgtaa ttctggcgca
     2461 gaacacgttt ggtctttcca gtgatttgga tccgcttttg gccctggccc gccactaccg
     2521 cctgcaggtg atcgaagact gtgcgcacgg gttgggtggt cagtacaaag ggcagcccaa
     2581 tggaaccgtg gccgacgcgg cgtttttcag cctgcagtgg tcgaaaccaa tctcgacggg
     2641 tctggggggg attgccgtca ccagcgttcc tgaaatcgct gaaaaattag cagtagcaga
     2701 gaaggcgtac ctggccccca ggttgttcga gtcactatca ctccgactcc tggtggcagg
     2761 gtacgaccga tttttcagcc cccggttctt ctggcgggct caacatgcct atcgacgact
     2821 cagccagtgg ggcgttgtgc ccggttcttc gacctgggga gaagttaatg gggtggtcaa
     2881 accccgaaat tttgaaaagc ggtacccggc tattttggca cgacgcggca accacgcact
     2941 gcaacactta gttcaaacga tagcacatag acagagggta gccgatgcct atgacgagat
     3001 tttgcgacaa atcgatcggg tcactcccgt tcaaccagct tatgcgatcc atgccttcct
     3061 ccgctatccc ttggaagtgc gtaatcgctc cggtttcttg gcagaggcgc aaaaaaggta
     3121 tattgaagtt ggtgattggt tccgttctcc cttgcatccg gtagaaggcg atctgcgacc
     3181 ttggggatac cagtggggct gcgggccagt gggggagaac gtaagccggc acatcgtgaa
     3241 cttgcccacg caccgccacg tcagcccaaa cgaaatcggt cggattggcg actttctgat
     3301 ggatatgaaa aagcgggata tcgtctaagt gtcttcccga acagacgacg ggtcgctgga
     3361 tttggtccct ccccctggtg gcggggagcc ctcagctgag gtggcgcgga ttaaggccat
     3421 gtatgcccgg cgcgattctg accggacgct gggtgcgcgc taccatcctt tggctcccgg
     3481 caatctcttt ctgactcagg gattggagcg ggttattctg ctcgaactcc gacgggctgg
     3541 agtgctcgat ttggccaagt tgcggattct tgaggtgggc tgcgggaacg gtttgcgact
     3601 tcgcgatttc gtcaactggg gtgccgaccc aactcggctc gtcggcgtgg acatcactcc
     3661 cgagcgaatc gcgcaggccc ggcgcctttc cccgaacata accttcgtat gcgccaacgc
     3721 agaccgactg gaattttcca acggctcttt tgacttggtc tttcagtttt ctactttcac
     3781 gtcacttttg gattatgcca tgaaacgacg ggtagcggag gaaatgttgc gggttttggc
     3841 gaacgacggt tgtatccttt ggtacgactt tcgggtgcgg ggtcgagagc gggagcattc
     3901 cgcccccatc ggtcgccggg aaattcgtga actgtttgcg gagggtacag ttcggctgcg
     3961 gtcgatgact ttagcaccgc cgttggctcg gcgattggcg ccctactggt ggaccgggtg
     4021 tcttcttctg gaaaaaattc cgctcctgcg tacccattgg tttggagtga tccgtaaaaa
     4081 aaggccgacg gcatgacccg gtcaaagatt gtgtcagaca agcagcctga agcacgccag
     4141 ataccattct atcaaccctc catcaccgag cgagaaatca gggcagtggt gcaaaccctg
     4201 cgttccggtt ggctcaccac cgggccgaaa accaaaaaat ttgaaaaaag atttgcgaga
     4261 tatgtcggcg ccaaacacgc cgtcgcgctc aactcctgta ccgccggctt gcacctggct
     4321 ctggtggcct ccggcgttgg gccgggggat gaagtcattg tgcccaccta cacttttgca
     4381 gcttcagccg aaacggtcat ccacgcgggc gccaagcccg tcttggtcga ttccgaatcg
     4441 gatacgctca acctttccgt ggcggaggtc gaaaaggaaa tctcccccag gaccaaggcg
     4501 gtggtcgcgg tggatatcgc tggcctaccc tgtgattacg cagcgttgca gccagtggtc
     4561 aagaagcagg gcatacatct gattgatgac gccgctcatg ccctgggggc atcatatgat
     4621 gggcggatgg tcgggaccct ggctgatctg accgccttca gtttttacgc cacgaagaac
     4681 cttaccacgg gtgagggggg catggtcacc acggacaacc aggaactggc ggaaaaaatt
     4741 tctcttttaa gtctgcacgg gatgtcgcga gatgcttgga aacgctaccg taccggcggg
     4801 tcgtggtact acgagcttct ggatgccgga ttcaaataca acttcaccga tctccaggcg
     4861 gccctgggat tgccccaatt ggcccgcctg cgtaccttac aggcacagcg ccgggcgcgg
     4921 gcgacgctct atgatcggga attaggagat ctgcccgaag tgaggcttcc ggcgcaaaaa
     4981 aaaggacggg tacacgcctg gcacttgtat atcattcggt tgcggctgga gcagcttaaa
     5041 atcgaccgga atcaattcat ccatgagtta acccggcgca ggatcggtac cgctgtgcac
     5101 ttcatcccgc tgcacctcca cccgtactac cgggagcggt acggctacca gccggaagat
     5161 tttccggtgg cacatgacaa ctaccagcgc gccattacgc tgccgttgta tcccaggatg
     5221 acgcgagcca tggtgtgcta cgtcgccggc gcagtgcgtg aaatcgtgca gaagtaccga
     5281 catcggggga gagggtgagc gtcacgccgc ccgttatcgc caaaaagctc cgcaataccg
     5341 tgctcctgtt tctgggcgat caggcggtcc tgtggggagc tttcctgttc gccctctggc
     5401 tgaaaggact cacgaggaat ctcgggtttt tcgattacct tgagccgggt ttgctcctgg
     5461 ccggcctctg ggtcgttatt tttctcgtct cgggaatcta ctggcgcggc tcacgatcct
     5521 ttctccatga acttctgcgc attaacaatg ccgtggcgct gggtattctc attctccttt
     5581 tagccacgtt cgacgtcacc agccccctct cccaggtgcg ggtgaccctg ctcggctact
     5641 gggcggtgct cctggcgggg ctctggcttt tccgctggct cttctggcag aaattcaatc
     5701 gtcaattggt ctccgggttt cccgaactgc cgaccccttt ggtcctcctg cgccgtctcg
     5761 gcattctggg gatggatttg gccttggccg gcctctcgct tttcctcgcc tttcttttgc
     5821 gctttgacgg gcgcattccc gccaaccatc tgaccatttt tttctacctt ttgccgctgg
     5881 tggtgattat ccggttttcg gctttttctc tggcgggact gtacagcggc ctgaaccgct
     5941 acgccagcat tgccgatttg ttcacggtgc tcaaaggagt cagtttcggc actgccctgt
     6001 ttgttttgcc ggccgcggtg cagtggctgg tgaaccacct cgatccgaaa gtggcctcaa
     6061 ccttggggct gacctggttg ctcccgttgt tccccaggct gcacgggtac ccgcggtcag
     6121 tctttttcat cgactggctg ttgatggtga tcttcatcgg tgggtcgcgc tttgccatcc
     6181 gggcgctgcg ggagctggcg ccgaattttc tgcgcgaagg caaacgcacg ttgatcgtgg
     6241 gggcgggaaa cgccggggag atgcttatgc gcgaaattcg gcgcaaccgt tcgctcccct
     6301 acctgcccgt cggcttcatc gacgacgacc cgaacaagcg cggcctgcgg gttcacgggt
     6361 tgccggtggt cggcagccac cgcgaaatcc cgcagttggt atcccgcctg ggagccgaac
     6421 aaatcgtcat tgccattccc tcggccaccg ggatccaaat gcgccggatc gtcgaggcct
     6481 gtcagcagag caaagtcgag atccaaacgt tgccgcccct gaaggaaatt ctgggggggc
     6541 aggtggacct gcaccaggtg cggaaagtgc gtctggaaga cctcctgggc cgcgaaccgg
     6601 tgcagattga gaccggacaa attgccagtt tcctggaagg ccgccgggtc ttgatcaccg
     6661 gcgcggcggg ctccatcggc gccgaactgg tgcggcaagt tttacgcttc cgtccccttc
     6721 aggtgctggc ggttgaccgg gccgagaaca atgtctttgc gctggagcag gaaatcgccg
     6781 cccgcactga ccgctcccgg gccatactct ctatcgggga tgtgaccgac ggcatccgga
     6841 tggacgacct gttcgcttct tttcggccgg aggtggtgtt ccacgccgcg gcctacaagc
     6901 acgtgcccct gatggaagat caccccgacg aagcggtgaa aaataacgtg gccgggacga
     6961 tcggcatggc cgacctggcc gaacgctacg gcactcgaaa attcgttttc atttccaccg
     7021 acaaggcggt ccaccccacg agcgtgatgg gggcaaccaa gcgggtggcg gaactgtacc
     7081 tgcaggctaa gccggctgca cccactgcgt tcgtctccgt ccgcttcggc aacgttctgg
     7141 gctccgacgg gagcgtggtg ccgctcttcc agaaacaaat cgcgagaggc gggccggtga
     7201 ccgtcaccca tccggaggtg agccgctttt tcatgaccac gagcgaagca gtgcagttgg
     7261 tcttgcaggc cgcctccatc gggcaggccg gcagcgtgct gcttctggac atgggggagc
     7321 cgatcaaaat tctcgacttg gcctgcgacc tgatcaccct ctccggcta
//
LOCUS       DBNGZD010000289         1552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_741946, whole genome shotgun sequence.
ACCESSION   DBNGZD010000289 DBNGZD010000000
VERSION     DBNGZD010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_741946"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..328)
                     /gene="rpsJ"
                     /locus_tag="AC16GM_07690"
     CDS             complement(20..328)
                     /gene="rpsJ"
                     /locus_tag="AC16GM_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR2923592.1"
                     /translation="MPGQKIRIRLKAYDHHSLDQSTKEIARTVLRTGAKLVGPIPLPT
                     ARSVYTVLRSPHVDKKSREQFETRIHKRLIDIYDSTPQTVDALIKLELPAGVDVEIKV
                     "
     gene            complement(534..>1552)
                     /locus_tag="AC16GM_07695"
     CDS             complement(534..>1552)
                     /locus_tag="AC16GM_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR2923593.1"
                     /translation="LLMHANKREEVEEVYAGDIAAAVGLRKTSTGNTLCDPDHPILLE
                     KMVFPDPVIFVAIEPKTKADQEKLSEALARLADEDPTFKVRTDEETGQTVISGMGELH
                     LEILVDRMLREFKVQANVGRPQVAYKETITKAVEAEGKFIRQTGGRGQYGHVVIDLEP
                     LPPGKGFVFENKVIGGTVPREYVPAVERGIRDALENGVLAGYPLVDLKAVLTDGSYHD
                     VDSSEIAFKIAGSMALQDGAKKAGPKLLEPVMNVEVVVPETYLGDVMGDLQARRGKIG
                     GMFPRADGQVVAATVPLAEMFGYATKLRSLSQGRAVFTMQFAHYEEVPKSVAEEIVAK
                     VRGV"
BASE COUNT          277 a          479 c          441 g          355 t
ORIGIN      
        1 gctctctgtc tccgaaaaac taaactttga tctccacatc cacgccggcc ggcagttcca
       61 gcttgatcaa ggcgtcgacg gtctgcggcg tcgagtcgta gatgtcgatc agacgcttgt
      121 gaatccgcgt ctcgaactgc tcgcgcgatt tcttgtccac gtgcggcgac cgcaaaacgg
      181 tgtagaccga gcgtgcggtc ggcaacggga tgggaccgac caatttcgcg ccggtgcgca
      241 gcaccgtgcg cgcgatctct ttggttgact ggtccaagga gtggtgatcg tacgccttga
      301 gcctgatcct gattttctgt cccggcattt cgctttctct tctcagcgcg cgggattcga
      361 ttccccgcgc tcctgagttc atccgttccc gcgcggggac ttctgcccgc cgctcaccga
      421 accgtatatc tccgtcaaaa aagactattt ccctttttat ccctaccgtg cttggcaagg
      481 gaaaccttta atgtatgaga aagagaaaaa agcgcaagtg cttttttgcg gtcttaaacc
      541 ccccgcacct tcgccacaat ttcttcggcc accgatttgg gcacttcctc atagtgggcg
      601 aactgcatgg tgaagaccgc ccggccctga gagagggagc gcaatttggt ggcgtagccg
      661 aacatttccg ccagaggcac ggtggcggcc accacctggc cgtcggcccg ggggaacatc
      721 ccgccgatct tcccgcgccg cgcctggagg tcgcccatca catcgcctaa ataggtttcg
      781 gggaccacca cttcgacgtt catcaccggc tctaagagct tcggcccggc tttcttggcc
      841 ccgtcttgca gggccatcga cccggcgatt ttaaaagcaa tttccgacga atcaacgtcg
      901 tggtacgacc cgtcggtgag caccgccttc aggtccacca aggggtagcc ggccagaacg
      961 ccgttctcca gtgcgtcgcg aatcccgcgc tccacggcgg gaacgtactc gcgcggcacc
     1021 gtgccgccga tgactttgtt ctcgaagacg aaaccttttc ccggtgggag cggttccagg
     1081 tcgatgacga cgtggccgta ctgcccgcgg ccgccggttt gccggataaa ttttccttcg
     1141 gcttccaccg ccttcgtgat ggtttccttg taggccacct gcggccggcc cacgttggcc
     1201 tggactttga attcccggag catccggtcg accagtatct ccagatgcag ttcgcccata
     1261 cccgagatga ccgtttggcc ggtctcctcg tcggtacgga ctttgaaggt ggggtcctca
     1321 tccgccaggc gggccagcgc ttcggagagt ttttcctggt cggctttggt tttgggttcg
     1381 atggcgacaa aaatcaccgg atcgggaaaa accatctttt ccagaagaat cgggtggtcg
     1441 ggatcgcaga gggtattgcc ggtggaagtt ttgcgcagac cgacggcggc ggcgatatcg
     1501 ccggcgtaca cttcttcgac ttcttccctt ttgttggcgt gcatcaaaag ca
//
LOCUS       DBNGZD010000290         5255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1922160, whole genome shotgun sequence.
ACCESSION   DBNGZD010000290 DBNGZD010000000
VERSION     DBNGZD010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1922160"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC16GM_07700"
     CDS             <1..187
                     /locus_tag="AC16GM_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HIR2923594.1"
                     /translation="VVYFIGIDKVRFRRPVLPGDQLRFEVTMLAYRHNTCKMEGKAFV
                     GDELVTEAMLMATVVDR"
     gene            234..1034
                     /gene="lpxA"
                     /locus_tag="AC16GM_07705"
     CDS             234..1034
                     /gene="lpxA"
                     /locus_tag="AC16GM_07705"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR2923595.1"
                     /translation="MTIETEIFAPPRIHPTAVVNPRAQIAEDVLIGPYAIIGRDVEID
                     SGTTVGSHALVDEGARIGKSVQIHHGAVVSGRPQDLKYQGEPTHLFVGDGTIIREFAT
                     LHRGTTHTGKTVVGLNCLIMAYAHVAHDCRIGDNVILANAVNLAGHVTIEEWAIVGGV
                     VPVHQFVRIGAHAMIGGGFRVPMDVVPYALAGGYPLKIEGLNKIGLKRRGFPEPTIKA
                     LRQAFRILFQAKMNTTPALERIRAEVEHLPEVQRLVRFVAESERGITK"
     gene            1067..2098
                     /locus_tag="AC16GM_07710"
     CDS             1067..2098
                     /locus_tag="AC16GM_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR2923596.1"
                     /translation="MSKSISVGVIGVGHLGRHHARIFAALEGVVLAGLYDRDYPKTKR
                     VAGELGVPAFANLQALFDQVQVVSIVVPTTAHAEIACAALACGKDVFIEKPISATLPE
                     ADRIIALSQEKQALVQVGQIERFNPCFAALENFPLDPLFIEVHRLAGFSPRGLDVAVV
                     LDLMIHDIDLVCHLVKAPLENVQAASAAVISDSDDIANARLVFANGCVANLTASRISP
                     KPMRKVRIFQKNAYLSLDLADKKVDLYELVTNADGSLPPQAGVAGGSTVPLFQFPVEE
                     GGRAITFRQPPVPEYDMLTRELESFVRAIRTRSAPAVSAQEGRRALEVALTVLQKGQA
                     QRRRTGIPV"
     gene            2135..3274
                     /gene="lpxB"
                     /locus_tag="AC16GM_07715"
     CDS             2135..3274
                     /gene="lpxB"
                     /locus_tag="AC16GM_07715"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR2923597.1"
                     /translation="MIVAGEASGDLHGALLVRELQKRRPDFFFFGLGGDQMHEQGVRL
                     AYHVRALSVMGFFEVVRRIGFFRKVFREVLRAVEQQKPTAAVLIDYPGFNLRLAKALK
                     ARGIPVIYYIAPQIWAWRPQRIKQIKRFVDLMLVILPFEEELYRTNGVSVRFVGHPLV
                     DQVRPSGTREGFRQKWNLAPDRPLLGLLPGSRPQEVAHHLPVMLKAAALLARQGVTCQ
                     PVIGLAPTINRLMVDEILRSAGRDPKSNSTYRVVEQATYDLMCYAKLLVVSSGTATVE
                     AALAGTPMVVLYKTSPFTYLLARRMVRIPRIAMVNVLAREELVPEFIQKQATPEAVGQ
                     AMVQLWQDEVRYRRIQERLAEVKASLGNPGAAGRAAEAILSHLSL"
     gene            3281..4576
                     /locus_tag="AC16GM_07720"
     CDS             3281..4576
                     /locus_tag="AC16GM_07720"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF016311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR2923598.1"
                     /translation="MGTSAIFFLYNFMWTLGLIFIFPYVVLRSLFDRTGVLERVGRYG
                     WPPITTEARRPLIWLHAASVGEVKALGQFTLLLQRHFPQAQFCLSTFTRQGKIEARKI
                     LPNVSTFYLPLDHPWPVRRAVAHLKPALLILAETELWPNLIIKAHQAGAHVFLVNGRI
                     SASSFPRYRRFRRLFAPALTAMERCWVQSDIDRARLVSLGAPAERVEVVGSLKFDHQI
                     KAGLPRLRQEVRNRLHLAPDRPVVVAGSTRPGEEELVIEAFASLRQKFPQALLIIAPR
                     HLKRLGAVEILLQQKQLAAQRLGVYRQNGSGAEDLSVLVVDSMGELALLYAAGDVAFV
                     GGSLVDYGGHNPLEPAVVGTPVVFGPFTDHAQRAAEILLASGLGTEVRSVAELVLAIE
                     KQLLSRAQPPDGFARFQTLLERESGTAALCVEKLRPYLS"
     gene            4676..>5255
                     /gene="lpxK"
                     /locus_tag="AC16GM_07725"
     CDS             4676..>5255
                     /gene="lpxK"
                     /locus_tag="AC16GM_07725"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR2923599.1"
                     /translation="MRHWSYRWGVVRSVPLKTKVVSIGNISAGGSGKTPLVAYLARRL
                     AGEGKRVCVVTRGYRGKARALVALLPGERPARDWMEIGDEARWLQKELPAVPLVIHSK
                     KYQAARWADQNLNPDVLLIDDGFQHQRLTRDFDLVMVTPDDLDPNNHLLPRGRLRERP
                     QALARAGFIFLNATGGEDESLDLLDRIRRYAPE"
BASE COUNT          985 a         1566 c         1633 g         1071 t
ORIGIN      
        1 ggtggtctac ttcataggca tcgacaaagt gcgcttccgc cgcccggttt tgcccggcga
       61 ccagttgcgg ttcgaagtga cgatgctcgc gtaccggcac aacacctgca agatggaagg
      121 caaggccttc gtcggcgacg aactggtcac cgaagcgatg ctcatggcga ccgtcgtgga
      181 tcgataagaa caggtgatgg gccgtaggtg gaaggtggta gggaaagacc acaatgacca
      241 ttgaaacgga aattttcgcc ccgcccagga ttcatcccac ggcggtggtc aacccgcggg
      301 cgcagattgc ggaggatgtg ctcatcggcc cgtacgccat tattgggcgg gacgttgaaa
      361 ttgattccgg caccacggtc ggctcccacg cactggtgga tgagggagcc cgcatcggga
      421 agagcgtcca gattcaccac ggcgcggtgg tgagcggccg gccccaggat ttgaagtatc
      481 aaggcgagcc gacccacctc tttgtgggtg acggcacgat tatccgggag ttcgccaccc
      541 tgcaccgggg aacgacgcac acgggcaaga cggtcgtcgg cctcaattgc ctgatcatgg
      601 cctatgcgca cgtggcccac gactgccgga tcggcgacaa cgttatcctg gccaacgcgg
      661 tcaacttggc cggccatgtc accatagaag agtgggcgat tgtcggcggg gtggtgccgg
      721 tgcaccagtt cgtacggatc ggcgcccacg ccatgatcgg cgggggcttc cgcgtcccca
      781 tggacgtggt gccgtacgct ttggccggtg ggtatccgct caaaatcgaa ggactcaaca
      841 aaataggact caagcggcgg ggcttccccg aaccgacgat taaagccctc cggcaggcct
      901 tccggattct ctttcaggca aaaatgaaca cgacgccggc tctggaacgc atccgggcgg
      961 aagtcgagca tctcccggaa gtccagcggc tggtccgctt tgtggcagaa agcgaacggg
     1021 gcataaccaa gtagtttttt ccggacctgt tccggtttcg gccgctatgt caaaatcgat
     1081 ttccgtcggc gtgatcggtg tcgggcacct gggccggcac catgcccgca ttttcgctgc
     1141 gctggaaggg gtggtcctgg ccggcctcta cgaccgcgat tatcccaaaa cgaaacgggt
     1201 ggcgggcgag ctgggcgttc ccgcttttgc caatctccag gcactcttcg atcaagtcca
     1261 ggtagtgagc attgtcgttc caactaccgc tcatgccgag atcgcctgcg ccgctctggc
     1321 gtgcgggaag gacgttttca tcgagaagcc gatcagcgcc accctgccgg aagccgaccg
     1381 gatcatcgcg ctgtcgcaag agaagcaggc cttagtccag gtaggacaga tcgagcgctt
     1441 caacccctgc tttgcggcgc tcgagaactt cccgctcgat ccgctcttca tcgaagtgca
     1501 ccggctggcc ggtttctccc cccgcgggct ggatgtggcc gtggtgctcg atctcatgat
     1561 tcacgacatc gatttagtct gccacctggt caaagcgcct ctcgagaacg ttcaggccgc
     1621 ttctgccgcg gtaatctccg actccgatga catcgccaac gcccggctgg tctttgccaa
     1681 cggctgcgtg gccaacctca ccgccagccg gatttcgccc aagccgatgc gcaaggtgcg
     1741 cattttccaa aagaacgcct atctctcgct cgatttagcc gataaaaaag tggacttgta
     1801 cgaactggtg acgaacgccg acggttccct cccgccgcaa gcgggagtcg ccggcggttc
     1861 aactgttccc ctatttcagt ttcccgtgga ggaagggggg cgcgcgatca ccttccggca
     1921 gccgccggtg ccggaatacg acatgctcac gcgggaactg gagagtttcg ttcgtgcgat
     1981 ccgcacgcgc agcgccccgg ccgtttcggc ccaggagggc cggcgagccc tggaagtcgc
     2041 cctcacggtg ctgcaaaaag gtcaggcgca acgccggcgg acgggcattc cggtctagcg
     2101 ctggagaaag ggaaaaagga cggcacaaaa aattttgatt gtggccgggg aagcatccgg
     2161 tgatctgcac ggggcgcttt tagtgcggga actccagaag aggcgtcccg attttttctt
     2221 tttcggcctg gggggcgacc agatgcacga gcagggggtg cgtctcgcct accacgtgcg
     2281 cgcattgtcg gtcatgggtt tcttcgaagt agtgcggcgg atcggatttt tccgaaaagt
     2341 gtttcgagag gttctccgcg cggtcgagca gcaaaaaccg acggcggctg ttttgatcga
     2401 ctaccccggg ttcaacctgc ggctggccaa agcgctcaaa gcgcggggga taccggtcat
     2461 ctactacatc gccccgcaga tctgggcctg gcggccgcaa aggatcaagc aaatcaaaag
     2521 gttcgttgac ctgatgctcg tgattctgcc gtttgaggaa gagctctacc gcaccaacgg
     2581 cgtgtcggtc cgctttgtgg gccacccatt ggtggaccag gtgcgcccgt ccggcacccg
     2641 cgagggattt cggcaaaaat ggaatttagc tcctgatcgt ccgcttttgg gtcttttgcc
     2701 cggttcgcgt cctcaggagg tggcgcacca cctgccggtg atgctcaaag ccgcagcact
     2761 cctcgcacgc cagggggtta cctgtcagcc ggtgatcggg ttagcaccga ccatcaaccg
     2821 gctaatggtt gacgaaattt tgagatctgc cgggcgcgat ccaaaatcga actcgactta
     2881 tcgtgtggta gagcaggcta cttacgattt gatgtgctac gcgaaactct tggtcgtttc
     2941 atccggtacg gcgacggtgg aagcggcctt agccggcaca ccaatggtcg tgctttacaa
     3001 gacttcgcct ttcacctatc ttttagcacg gaggatggtg cgaattcccc gcattgcgat
     3061 ggtcaacgtt ctggccaggg aggagctggt gcccgagttt atccaaaaac aggccacacc
     3121 ggaagcagtc ggccaggcga tggtccaact ctggcaggat gaagtccgat accggcgcat
     3181 ccaggagcgg ctggcggagg tgaaggcatc gctcgggaat cccggagcgg ccggccgtgc
     3241 ggcggaggct attttaagcc acctttccct ctgaacgccc atgggtacct cggccatttt
     3301 ctttctttac aacttcatgt ggacgctcgg cctcatattc atctttcctt acgtcgtcct
     3361 gcggtcactt tttgaccgga cgggggtgct ggaacgagtc ggtcggtacg gctggccgcc
     3421 aatcactacg gaagccaggc ggccgctcat ctggctgcac gccgcgtcgg tgggtgaggt
     3481 gaaagccctg gggcagttca ccctgctcct gcagcggcat tttccgcagg cgcaattctg
     3541 cctttcgacc tttacccgcc aggggaaaat cgaggcgaga aagattttgc cgaacgtctc
     3601 cacattctat ttgcctttgg accacccctg gccggtgcgc cgtgcggtcg cccacctgaa
     3661 accggccttg ctcatcctgg cggaaacgga actctggccc aatctgatca taaaagcgca
     3721 ccaggccgga gcgcacgtct ttttggtcaa cggccgcatc tcggcctcct ctttcccccg
     3781 ttaccggcgt ttccggcggc tctttgcccc ggccttgacg gccatggagc ggtgctgggt
     3841 ccagagcgat atcgaccggg cgcggctcgt ctctctgggc gcaccggcag agcgggtgga
     3901 ggtggtgggc agtctcaagt tcgaccacca gatcaaagcc gggctgcccc gcctgagaca
     3961 ggaagtccga aaccgactgc atctggcccc ggatcgtccg gtggtggtgg ccggaagtac
     4021 ccggccgggc gaagaggaac tggtgatcga ggccttcgcc agcctccgac aaaagttccc
     4081 ccaagcactt ttgatcatcg cgccccggca tctcaagcgt ctgggggctg tagaaattct
     4141 cttgcagcaa aaacagttgg ctgcccaaag gctcggtgtc taccgacaaa acggcagcgg
     4201 agcggaagat ctctccgttt tggtcgtaga cagcatgggg gaattggctc tgctctatgc
     4261 cgccggtgac gtggctttcg tcggagggag tctcgtcgat tacggcggcc ataatccctt
     4321 ggagccagcg gtggtgggga ctccggttgt cttcgggcct tttaccgatc acgcccagcg
     4381 ggcggcggaa atccttttgg cctccgggct gggcaccgaa gtgcgctcgg tcgctgaact
     4441 tgtcctagcc atcgaaaagc aattgctctc ccgcgcccag ccgcccgacg gtttcgcccg
     4501 gttccaaact ctcctggagc gggaaagcgg caccgccgct ctctgtgtgg agaagttgcg
     4561 gccgtacctt tcctgagcgt tgatcgccat gaccgcgaat ctcctccaac gttaccggtt
     4621 tcttttttct ccattgctca gcgcgaccgc ggctcttttt cgagtgggtg ttggtttgcg
     4681 ccattggtcg taccgctggg gggtggtgcg gtcagtcccg ctgaagacga aagtcgtcag
     4741 tatcgggaac atcagtgccg gcgggagcgg gaaaactcct ctggtcgcct accttgcccg
     4801 gcgactggcc ggggagggca aacgagtctg tgtggtgacg cgcgggtatc gggggaaggc
     4861 gcgggcactg gtggcgctcc tgccgggtga acggccggca cgggattgga tggaaatcgg
     4921 ggatgaagcg cgctggttgc aaaaagaact tcccgctgtg ccgctcgtta tccactcaaa
     4981 aaagtatcag gcggcgcgct gggctgacca aaatctcaac cccgacgtgc tactgatcga
     5041 tgacggcttt caacaccagc gactcacccg agattttgac ttggtcatgg tcacgccgga
     5101 cgatctggat ccgaacaatc acctgttgcc gcgaggccgg ctgcgcgagc ggccgcaggc
     5161 attagcgcgg gctggtttca ttttcctcaa cgccaccggg ggcgaagatg aatccttaga
     5221 tttgctcgac cgtattcgca ggtacgcccc agaga
//
LOCUS       DBNGZD010000291         9975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1853400, whole genome shotgun sequence.
ACCESSION   DBNGZD010000291 DBNGZD010000000
VERSION     DBNGZD010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1853400"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /gene="selB"
                     /locus_tag="AC16GM_07730"
     CDS             <1..1828
                     /gene="selB"
                     /locus_tag="AC16GM_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR2923600.1"
                     /translation="VTRLTGIDPDRLPEEKARGMTIDLGFAWLALPSGLEVGIVDVPG
                     HERFVRNMIAGVGGIDAVLFVVAADDGWMPQSAEHLAILKLLGIQRGLIVLTKIDLVK
                     PDWRILVEEDIREKLKGSFLAQAPLVKVSAPTGEGVETLKQEIGSLVARLAPRRDLGK
                     PRLFIDRVFSMAGRGTVVTGTLRDGSLRVGEEIEILPTGLRARIRGLETHKKPVEQSG
                     PGARLALNLSGVDKEKLSRGDLIIKPGQGFLTRRWAASVALWEQSPFPLKDNSEVLFL
                     LGTSETRAKVHLLDRSVAQPGEEVLVEFTAPSPVAARIGDHFILRLPSPGVTLAGGKV
                     LELEPFWRKRRTPEVRAYLSERVSLAASDLLRSELEKSVVQPRTTLLFHSHIAPAEIE
                     AALLDLERQQLLWQWGGFVGLRQKIEGLQREIVTLVEKEHQTHPYRLGIVRAEMAGRL
                     RKNEAEIDPLIVQMLEKGLLVQEAGFLRLPPFSPRLLPEQERQVEKLLNEFEQSPYGA
                     PTKEEIFARGDGWKDLVAFLLYQKTLIECGDGVLLRALDWEAVTRKVTAFFKENGTMT
                     VGQLRDLLHTSRKYAVPILEKLDHVGLTRRVGDQRVLVDTHD"
     gene            1887..3332
                     /gene="gatA"
                     /locus_tag="AC16GM_07735"
     CDS             1887..3332
                     /gene="gatA"
                     /locus_tag="AC16GM_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR2923601.1"
                     /translation="MELNRLKAWELLPRLEKKEISATELVTQTLAEIGRTGKTLNTFI
                     TVTAEQALSQAKQIDARRGRGEKVGALAGIPIAIKDVICTQGIQTTCGSRILTGFVPP
                     YDATVITKLKVADAVIVGKTNMDEFAMGSSNENSAFGPVRNPHDPSRIPGGSSGGSAA
                     AVAAHQAIFALGSETGGSVRQPASLCGVVGLKPTYGAVSRWGLVAFASSLDQIGSFGR
                     DVRDCALLFDIIAGGDPRDSTSRKAYQPHCFEKLRQEPKSFRIGVPKEYFALGIEPDV
                     ERLVRGAIDRIGREGVPVEEVSLPSTDKGIATYYIIAPAEASSNLARYDGVKYGLRVY
                     GGNDDLLTMYGKTRQEGFRPEVKRRIMLGTYVLSAGYYEAYYLKAQKLRAQVKKDFDD
                     AFKKVDLLVCPTSPTAAFKLGEKVDDPLTMYLNDVYTVTANLAGIPAISIPCGKTKAG
                     LPVGLQILAPPFAEEKLFQFAHWVEGILKSS"
     gene            3334..3756
                     /locus_tag="AC16GM_07740"
     CDS             3334..3756
                     /locus_tag="AC16GM_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923602.1"
                     /translation="MRTSIKPSLITVLAILVFLGVLAFGVVKWYNGQEKKYSQVMVRI
                     LRSVSLPDTATFKDYAKVLRREIKPGFTVREVHAVMRGYDRIETFPFWETKFPSWKYF
                     LEYYRYDFPWIDEFFPGPTAIIIEYDSSWNFKSFATQP"
     gene            3787..5226
                     /gene="gatB"
                     /locus_tag="AC16GM_07745"
     CDS             3787..5226
                     /gene="gatB"
                     /locus_tag="AC16GM_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR2923603.1"
                     /translation="MTYEPIIGLEVHAQLLTHSKIFCGCSTDFGSPPNSQTCPVCLGL
                     PGVLPVLNRKAVEYAMKMILAVGGQVQPKSIFARKNYFYPDLPKGYQISQFDLPLGKG
                     GAVEIEFDDSHKSIGITRIHLEEDAGKSLHPEGNPDRDYSLVDMNRCGVPLIEIVSQP
                     DLRSPREAYLYLLKLKQILEYLEICSGNMEEGSLRCDANVSVRPVGREKFGTKTEVKN
                     MNSFKAVERALTHEIERQTNLLESGREVIQQTLLWDADREEVEPMRQKEESDDYRYFP
                     EPDLLTLTVDADWVNTVKATLPELPDQKKNRFVTQYELSTYDAEVLTGEKAIANYYED
                     YVALYPKPKVVANWVMGEVLRELKERKITIQEFAVPPRQLASLLEKIDAGVLSAKMAK
                     EVFTEMAATGDAPEAIIQKRGLVQVSDTAELEKVVEAVLAENPENLKRYREGKDKLFG
                     FFVGEVMKKTKGQANPQVVNTILKSKLAQ"
     gene            5264..5869
                     /gene="purN"
                     /locus_tag="AC16GM_07750"
     CDS             5264..5869
                     /gene="purN"
                     /locus_tag="AC16GM_07750"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR2923604.1"
                     /translation="MKPLPLAVFASGEGTNLQAVINAIESGRLAAKIQFVLSNNSRSG
                     ALARARKHNIPAIHLSEVTCGSREEFVEKLKELLSGNSVELIILAGYMKLVPKEVIFL
                     YRNRIINIHPALLPRFGGPGLYGRRVHEAVLASGVKESGASVHLVDELYDHGPVILQR
                     TVPVKPDDTPETLAARVRGVEHEILVEAIDLFVQGKVPVSS"
     gene            5876..7510
                     /locus_tag="AC16GM_07755"
     CDS             5876..7510
                     /locus_tag="AC16GM_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2923605.1"
                     /translation="MKHIPPFSRNSTAVGPPFFFLILLALWVAAPVLAKTNRSIRRIQ
                     IERRDIFADGDSLLWYERLANRLHVLTKAGVIRRELTFREGEVLHPWFLEESERNLRQ
                     LGFLGTVKITADTLNDSGCTVRVRTQDQWSTELAASAQGGGGAYVLRLSGSEKNLFGW
                     GKTIEAAYEKTDLRNQWNASVLDPRLFGSRLSAELAYEKTSEGKAVAGRLALPLFSIR
                     SRYGFSLALSRQTDILRFFDKGEEAIFYNFRMINFSFRAVRSFGDSLKFDPFFLTEYA
                     ERDFSGPFRFAGFAGDPASVVANYVFPEPKATRLGLGLQVHRSRFMEGRFYDNFGNVE
                     DIGLGPSGEISFAGSHQRLNSTRTRWSPALLLNHTRSFGSAHLVRLTFSVENDFLSDR
                     WEGARTSARFIHYWRWGTRQTLAWQIRYVAITGVNRVGQLLLGGDNGLRGYDARRFSG
                     NKRLLVNFEQRLFAPVEILTVGLGAVAFVDFGSAWSPGTAPALSSLRSDAGVGLRLGL
                     LKSYGFKVLRLDWAKALNEPGSSFSFGTGMTFGLNL"
     gene            7507..9003
                     /locus_tag="AC16GM_07760"
     CDS             7507..9003
                     /locus_tag="AC16GM_07760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR2923606.1"
                     /translation="MNGQRRPLRWFLFILAVSILVKFFLIFRVPLTPQETYYWNYACH
                     LALSYFDHPPLGAWTIAFFRWLIGDSAFAVRIGALGYSTGLAIGLFLLGKRFLNDRLG
                     LTTFLSITLTPMFWLGGGVMTPDAPLLCFWVWAFYFFLQALQKKNLTSWVLFGFCTGA
                     SGLSKYTAVFIPVAVLFSITLSRNLLVIKSKGLWLSVLTAGAVFSPVLIWNAQHHWAS
                     FLFQTSRRAGELGSFSITPFLQFLGSQAGVISPLTLAAFLFAPVWALVSGWRNRRTDL
                     LILFSWSFPIIFFFALVATRYWVKVNWVTPGYLAAALAYSFFCGGRDPGGSSKARRRW
                     YGASVGLGFLFVLLLTVAVASPHIALGTKADSLTGWKELAGRVDSLRQEKGGAKKIFV
                     VGYEYKVASELAFYLPDKPETYSNTVVGERGLQYDFWFDEKKLLGKDALFVYDVRNRY
                     WTPEKLSLFFARVEPADSLPVFRAGKAVTTFYIFKCSQYLGVGGRTSG"
     gene            9129..>9975
                     /locus_tag="AC16GM_07765"
     CDS             9129..>9975
                     /locus_tag="AC16GM_07765"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR2923607.1"
                     /translation="MTTSAPQEAILDRKESQPLGAAVLTTEVTDYPLYARGKVRDIYD
                     LGDRLLFVATDRISAFDVVLPTGIPGKGKILTGLSVFWFDFLKKIVPNHFITADAREY
                     PKPLKKYESILAGRSMLVKKVKRIDLECVVRGYIAGSFWKEYVEARKISNAGNIILHG
                     EVLPDNLKESEILPFPIFTPATKADTGHDENISLAEAQKVVASETVLKLREVSLRLFQ
                     KAADYAQKRGIIIADTKFEFGRLGSQLILIDEVLTPDSSRFWPAQKYEPGRPQESFDK
                     QYVRDY"
BASE COUNT         2061 a         2859 c         2805 g         2250 t
ORIGIN      
        1 ggttacacgc ctcaccggca tcgaccccga ccgcctgccg gaagagaaag cccgcgggat
       61 gacgattgac ctgggcttcg cctggctggc gcttccttcc ggtctggaag tggggatcgt
      121 cgacgtcccc ggccacgagc gttttgtccg gaatatgatt gccggggtgg ggggcatcga
      181 cgccgtgctc ttcgtggtgg ccgccgatga cggctggatg ccccagtccg ccgaacatct
      241 ggcgattctg aaacttctgg ggatccagcg ggggctgata gtcctcacga aaatcgatct
      301 ggtaaaaccg gactggcgca ttttggtgga agaagacatc cgggagaaac tcaaaggaag
      361 ttttctcgct caggctcctc tggtaaaagt ctcggctccc accggtgaag gggtggaaac
      421 cttaaagcag gagatcggtt ccctggtggc gcgcctggcg ccccggcgcg atctcggcaa
      481 gccgcggcta ttcatcgacc gcgtcttcag catggccggg cgcgggacgg tggtcaccgg
      541 cacgttgcgc gacggttccc tcagggttgg cgaagaaatc gaaattctcc ccaccggttt
      601 gcgcgcgcgg attcgcggtt tagaaaccca caagaagccc gtggagcaaa gcggccccgg
      661 cgcacggctg gcgctcaacc tctcgggcgt tgacaaagaa aaattgtccc ggggagattt
      721 gattatcaaa ccaggccagg gctttttaac ccggcgctgg gccgcgtcgg ttgcgctctg
      781 ggagcaaagc ccgtttccgc tcaaagacaa tagcgaggtg ctcttccttt tgggcacgag
      841 cgaaacgcga gccaaagttc acctcctcga ccggagcgtt gcccagccgg gtgaggaggt
      901 tttagttgag tttaccgctc cctcaccagt ggccgctcga atcggcgatc acttcatcct
      961 gcggctgccc tcgccgggag ttaccctggc tggcggcaag gtgctggaac tcgaaccttt
     1021 ctggcgcaaa cggcgtacac cggaagttcg ggcgtatcta tcggaacgtg tttcgctggc
     1081 ggcaagcgat ctcctccgtt ccgagttgga aaaaagtgtc gtgcaaccgc gcaccacttt
     1141 gctctttcac agccacatcg ccccggctga aatcgaggcg gcgcttctgg atttagagcg
     1201 acagcaactc ctctggcagt ggggaggctt cgtcggtctc cgccaaaaaa ttgaagggct
     1261 tcagcgtgaa atcgtcaccc tggtggagaa agagcaccaa acccaccctt accggctcgg
     1321 tattgtccgg gctgagatgg ccggtcggct gcggaagaac gaagcggaga tcgatccgct
     1381 gattgtccaa atgctggaaa aaggtctgct cgttcaggag gccggtttcc tccgtttgcc
     1441 ccccttttcc ccgcgtcttt tgccggaaca ggaacgacag gtcgaaaagc tattaaacga
     1501 atttgaacaa tcaccttacg gcgcgcccac caaagaagag attttcgccc gcggcgacgg
     1561 gtggaaagat ctggtggctt tcctgcttta ccagaagacc ttaatcgaat gcggcgacgg
     1621 cgtgttgcta cgcgcccttg actgggaagc agtcacccga aaagtgacgg catttttcaa
     1681 agaaaatggc acgatgacgg tcggccagct tcgcgacctc ctgcatacct cgcgcaaata
     1741 cgctgtgccg attctggaaa aattagacca cgtaggtctc acccggcggg tgggggatca
     1801 gcgcgttctg gtcgacaccc acgactgagc ttttcgttct accgcaaata ttttttccat
     1861 caactaacat ctgctatatt ctccgtgtgg aactcaaccg gctcaaagcc tgggaactcc
     1921 tccctcgtct ggagaaaaaa gaaatttccg ccaccgaact ggtcacgcag acgctggcgg
     1981 aaatcgggcg cacgggcaaa acgctcaaca ccttcatcac cgtcaccgct gagcaggcgc
     2041 tgtcccaggc aaaacagatc gatgcccggc gcggtcgagg cgaaaaggtt ggtgctctgg
     2101 ctggaattcc gatagcgatt aaagatgtca tctgcactca aggcatccag acgacctgcg
     2161 gttcgcggat tttgaccgga ttcgtcccgc cctatgatgc gacggtgatc accaaactca
     2221 aggttgccga cgcggtcatc gtcggtaaaa ccaatatgga tgaattcgcc atgggttcgt
     2281 cgaacgaaaa ctccgctttc ggcccggtgc gcaacccgca cgaccccagc cgcattccgg
     2341 gcggttcctc gggtgggtca gccgcagcgg tggccgcgca tcaagcgatt ttcgccctcg
     2401 gctccgaaac cggtgggtcg gtgcgccaac cggcctctct ctgcggcgtg gtaggactca
     2461 agccgaccta cggcgccgtc tctcgctggg gattggtggc tttcgcttcc tctttagatc
     2521 aaatcggctc cttcggcagg gacgtgcgcg actgtgcgct tctcttcgat atcatcgccg
     2581 gcggcgaccc gcgcgattca acctcgcgga aggcatacca gccgcactgc ttcgaaaagt
     2641 tgaggcagga gccgaaaagt ttccgtatcg gcgtcccgaa ggaatatttt gccctgggga
     2701 ttgagccgga cgtggagcga ttggtgcggg gcgccatcga ccgcatcggc cgggaaggcg
     2761 tgccggtcga agaagtctcc ctgccctcca ctgacaaggg gatcgccacc tactacatta
     2821 tcgctccggc tgaggcgtcg tccaacctgg cgcgatacga tggggtcaaa tacggcctgc
     2881 gggtctacgg cggcaatgac gacctgctaa cgatgtacgg caagacccgg caggaggggt
     2941 tcaggccgga ggtcaagcgc cgcattatgc tcggcaccta cgtactttcc gccggctact
     3001 atgaggccta ctacctcaaa gcccagaaac tccgggcgca ggtgaaaaaa gatttcgatg
     3061 acgcgttcaa aaaagtcgac ctgttggtct gcccgacttc gcccaccgcg gcgttcaaat
     3121 taggggagaa ggtggatgat ccgctgacca tgtacctgaa cgacgtttat accgtcacgg
     3181 ccaacttagc cggcataccg gcgatttcga tcccttgcgg gaaaaccaaa gcgggcttgc
     3241 cggtagggtt gcaaattctg gctccgcctt ttgctgagga gaagttgttt cagtttgcac
     3301 actgggtgga gggaattctt aaaagtagct aatatgagaa cgtcaataaa accatcttta
     3361 attaccgttt tggcaattct ggtctttttg ggagtgctgg cattcggggt ggtgaagtgg
     3421 tataacggtc aggagaaaaa atacagtcaa gttatggtac gtattttaag aagcgtgtca
     3481 cttcccgata ccgcaacttt taaggactat gcaaaagtgt tacggcgaga aataaaaccg
     3541 ggttttactg ttcgtgaagt ccatgccgtt atgaggggtt acgatagaat tgaaacgttc
     3601 cctttttggg aaactaagtt tccttcttgg aaatattttt tagaatatta tcgatatgat
     3661 tttccttgga tcgacgagtt ttttccaggt ccaacggcaa tcataattga gtatgattcc
     3721 agctggaact tcaagagttt tgcaactcag ccttaggtaa agtcgtccgt aaccgaccga
     3781 aatataatga cctacgaacc aatcatcggt ttagaagtcc acgcccaact cctgacgcac
     3841 tccaaaatct tctgcggctg ctcgaccgac ttcggcagtc cgcccaattc ccagacctgt
     3901 ccggtctgcc tcggcctgcc cggtgttttg cccgtcctga accgcaaggc ggtggagtac
     3961 gctatgaaga tgatcttggc ggtagggggc caggtgcagc ccaaaagcat ctttgcccgg
     4021 aagaactact tttaccccga cctccccaaa gggtatcaaa tctcgcagtt tgatttaccc
     4081 ctcggcaaag gaggggcggt cgaaattgaa tttgatgact cacacaagag cataggcata
     4141 acccgcattc acctggagga ggacgccggc aaatcgcttc acccggaggg aaaccctgac
     4201 cgcgactact ctctcgtcga tatgaaccgc tgcggcgtgc ctttgatcga aatcgtctcc
     4261 cagcccgacc tgcgcagccc ccgcgaggcg tatctttatc tcttgaagtt aaagcaaatc
     4321 ctggagtatc tggagatctg ctccggcaat atggaagagg gcagtctgcg ctgcgacgcc
     4381 aacgtctcgg tccggccggt tggccgggaa aaattcggca ccaagactga agtcaagaat
     4441 atgaattcgt tcaaggcggt ggagcgggcg ctgacgcacg aaatcgagcg tcaaacgaat
     4501 ctcctcgaat cgggacggga ggtgattcag cagacactcc tctgggacgc cgaccgggaa
     4561 gaggtggagc cgatgcgcca gaaagaggaa tcggacgact accgctactt cccggaacct
     4621 gatcttctga ccctgacggt cgatgcggac tgggtcaata cggtcaaagc caccctgccg
     4681 gaactgcccg atcagaagaa aaaccgcttc gtgacgcagt acgagctctc gacgtacgat
     4741 gcggaagtgc tcacaggcga aaaagcgata gccaactact atgaagacta cgtggcgctt
     4801 taccccaagc cgaaggtcgt cgccaactgg gtgatggggg aggtcctgcg ggaactcaag
     4861 gagcgtaaga ttaccatcca agagtttgcc gtcccgcccc ggcagcttgc gtcgctctta
     4921 gaaaaaattg acgccggtgt gttgagcgcg aaaatggcca aggaagtgtt cactgaaatg
     4981 gcggccaccg gtgatgcgcc ggaggcaatc atccagaaac gcggattggt gcaggtttcc
     5041 gataccgccg aactggaaaa agtggtggaa gcggttttgg ccgaaaatcc ggaaaatctc
     5101 aaacgctacc gggagggaaa agataaactc ttcggcttct ttgtcgggga agtgatgaag
     5161 aaaacgaagg gccaggccaa tccgcaggtc gtgaatacca ttctcaaaag caaactggcg
     5221 caatgaacct cttgcccctt acggtaggat tcttttcccg gagatgaaac cgcttcctct
     5281 ggccgttttc gcttccggcg agggcaccaa tctccaggcc gtcatcaacg ccatcgaatc
     5341 gggacggctg gccgccaaaa ttcaattcgt cctttccaac aacagtcgat ccggcgcgct
     5401 ggcgcgcgcc cgcaagcaca acatcccggc gatacacctt tcggaagtga cctgtgggtc
     5461 gcgtgaagaa ttcgtggaaa agctcaagga actgctctcc ggcaatagtg tggaactgat
     5521 tattctggcc ggctatatga aactggtgcc gaaagaggta atttttctct accgcaatcg
     5581 gatcatcaac atccacccgg cgctcttgcc gcgcttcggc ggccccggcc tgtacggcag
     5641 gcgcgtccac gaagccgttc tggcttccgg ggtcaaagag agcggcgcgt cggtacactt
     5701 ggtcgatgaa ctctacgatc acggaccggt catcctccag cgcacagtgc cggtaaaacc
     5761 cgacgatact cccgaaacgc tggcggcccg cgtccgggga gtggaacatg agattctggt
     5821 cgaagcgatc gacctcttcg tccagggtaa agtccccgtt tcgtcctgag cgtcgttgaa
     5881 acacattcca cctttttcac ggaactccac tgcagttggc cccccttttt tctttttgat
     5941 tcttctggcc ctttgggtgg cggccccggt tttggcgaaa acgaaccgga gcatccgccg
     6001 catccagatc gaacggcggg atattttcgc cgacggcgac agtcttctct ggtacgagcg
     6061 tctggccaac cggcttcacg ttttgaccaa agcaggggtg atccgccggg aactgacctt
     6121 ccgggaaggc gaagtgctcc acccgtggtt cctcgaagaa agcgagcgga atctgcgcca
     6181 gctcggcttc ctcggcaccg tgaagatcac cgccgatacg ctgaacgact ccggctgtac
     6241 ggtgcgcgtg cggacccagg accagtggtc caccgaactg gcggcttcgg cgcagggcgg
     6301 cgggggcgcc tacgtcctcc gcttgagcgg ttcggagaag aatttgttcg ggtgggggaa
     6361 gaccattgaa gcggcctacg aaaaaactga cctccgcaac cagtggaatg cgtccgttct
     6421 tgacccgcgc cttttcggaa gccgcttgag cgcggaactg gcgtatgaaa aaacctccga
     6481 ggggaaggcc gtggccggcc gcctggcact cccccttttc tccatccgtt cgcgctacgg
     6541 tttcagcctc gccctctccc gccagaccga cattctccgt ttttttgata agggagagga
     6601 agcgatcttc tataactttc gcatgatcaa tttctcgttc cgcgccgtcc gctcctttgg
     6661 agacagtctg aagttcgatc cgttttttct gaccgagtac gccgaacgtg atttctccgg
     6721 cccattccgc ttcgctggtt ttgcgggcga cccggcgtcg gtcgtcgcca actatgtctt
     6781 tcccgaaccg aaagcgacgc gcctgggtct gggtctacaa gtccatcgca gccgcttcat
     6841 ggaaggccgt ttttacgaca acttcgggaa cgtggaagac attgggctcg gtccgtccgg
     6901 cgaaatctca tttgccggca gtcaccagcg cctgaattct acccgcacgc gctggtcccc
     6961 agctttgctc ttgaaccaca cccgatcgtt tggctcagcg cacctcgttc gtctgacctt
     7021 ttcggtcgag aacgactttt tgtctgaccg ctgggaaggg gcgcgcacct cggcccgctt
     7081 tatccactat tggcgctggg gcaccaggca gaccctggcc tggcagattc gctacgttgc
     7141 catcaccggc gtcaaccgag tcgggcaact gctcttaggc ggcgacaacg ggttacgggg
     7201 ctacgatgcc cgcaggtttt ccggcaacaa gcgtctcctc gttaatttcg agcagcgcct
     7261 cttcgctccg gtagagattc tgaccgtcgg cctgggggcg gtggcgtttg tcgatttcgg
     7321 ctccgcctgg tctccgggga cggccccagc gctttcaagc cttcgttccg acgcgggagt
     7381 gggcttgcgc ctggggttgc tcaagtcgta tggttttaag gtgctgcgtc tcgactgggc
     7441 caaggccttg aacgaaccgg gctcgtcttt cagtttcggc accggtatga cttttggttt
     7501 gaatttatga acggtcaaag gcgccccctc cgctggtttc tcttcatcct agccgtttcc
     7561 atcttggtga aattcttctt gattttccgt gtgccgctca cgccgcagga aacctactac
     7621 tggaactacg cctgtcacct cgcgctttcg tacttcgatc atcccccttt gggcgcgtgg
     7681 acgatcgcct ttttccgctg gcttatcggt gacagcgctt ttgccgtgcg tatcggtgct
     7741 ttgggatact ccaccggatt ggctattggc ttatttttgc tggggaagcg tttcctcaat
     7801 gaccggctcg gtctgacaac ctttctcagt atcaccctga cccccatgtt ctggctgggc
     7861 ggcggggtga tgactccgga cgcccccttg ctttgcttct gggtctgggc gttctatttt
     7921 tttctgcagg ccctgcaaaa aaagaacctt acctcgtggg tactattcgg cttctgtacc
     7981 ggggcgtccg gactctcgaa atacactgcg gtcttcattc cggttgcggt tttattctcg
     8041 ataacgctct cgcgaaatct tttggtcatc aagtccaaag ggctgtggtt gagtgtgctt
     8101 accgccgggg cagttttttc tccggtactc atctggaacg cccagcacca ctgggcctcg
     8161 ttcctctttc aaacctcgcg ccgggccggg gaactgggga gtttttccat cacgccattt
     8221 ttgcaattcc tgggctccca ggccggggtc atttcacctc taaccctcgc ggcctttctt
     8281 tttgctccgg tctgggcctt ggtttccggt tggcgcaatc gccggactga cctgttgatt
     8341 cttttttcct ggtcgtttcc gattatcttc ttttttgcgc tcgtcgccac gcgctactgg
     8401 gtcaaagtga actgggtcac gcccggctat cttgccgccg cgctggctta ttcctttttc
     8461 tgtgggggga gggatccggg aggcagcagc aaagcccgtc gaaggtggta tggagcctca
     8521 gtcggtttgg gatttctttt tgtcctcctt ttgacggtgg cggtcgcctc accccacatc
     8581 gctttaggca caaaggctga cagtctcacc ggctggaaag aactggccgg gcgggttgat
     8641 tctctgcggc aggaaaaggg tggggcaaaa aaaatttttg tggttggcta cgaatacaaa
     8701 gtggccagcg aattggcctt ctatcttccc gacaaacccg aaacctattc caacaccgtc
     8761 gtcggcgagc gcggcctgca gtacgatttc tggtttgacg agaaaaagtt gctcggcaaa
     8821 gacgcactct tcgtttacga tgtccgcaac cgctactgga cgccggagaa actttcgctc
     8881 ttctttgcgc gggttgaacc ggctgattcc ttacctgttt tccgagcggg aaaggcggtc
     8941 accacatttt acattttcaa atgctctcag tatctcggag ttggtggtcg aacgtccggc
     9001 taaagttccg cagcccggga ttgtatcctg ggctcgcgga tttttgttgg cagaacaggt
     9061 cgctccattt tccatttgcc tccacctgta ttatcttagg tcaaaccagt gaaaatcggg
     9121 gagccgacat gacgacaagc gcaccacaag aagcaattct ggatcggaaa gaatcacaac
     9181 cgctgggcgc cgccgtcctc actacggaag ttaccgacta cccgctctat gcccgcggga
     9241 aggtgcggga catctacgat ctgggcgacc ggctcctgtt cgtcgccacc gaccgcattt
     9301 ccgcttttga cgtggtcttg cccaccggca ttccaggcaa agggaaaatc ctcaccgggc
     9361 tttcggtctt ctggttcgat ttcttaaaaa agatcgtccc gaaccacttc atcaccgccg
     9421 acgcgcggga ataccccaag ccgctcaaaa aatatgagtc catcctggct ggccgttcga
     9481 tgctggtcaa aaaggtcaag cggatcgact tagagtgcgt ggtgcgtggc tacatcgccg
     9541 gttcgttctg gaaggagtac gtggaggccc ggaaaatttc aaatgcgggc aatatcatcc
     9601 tgcacggtga agtgctgcct gacaatttga aggaatcgga gatcttgcct tttccgattt
     9661 tcacccccgc caccaaagcc gacaccggcc acgacgagaa tatttcactg gccgaagccc
     9721 agaaggtcgt cgcttctgaa accgtgctca agttgcgcga agttagtctg cggctgtttc
     9781 agaaagccgc cgactacgcg caaaagcgcg ggatcatcat cgccgacacg aaattcgagt
     9841 ttggccgcct gggtagccag ttgattctga ttgacgaagt gctcaccccc gattcctccc
     9901 gcttctggcc cgctcagaag tacgaacccg gtcgcccgca agaaagtttc gataagcagt
     9961 acgtgcgcga ctact
//
LOCUS       DBNGZD010000292         5416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_603782, whole genome shotgun sequence.
ACCESSION   DBNGZD010000292 DBNGZD010000000
VERSION     DBNGZD010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_603782"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="AC16GM_07770"
     CDS             complement(<1..599)
                     /locus_tag="AC16GM_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2923608.1"
                     /translation="MSGPKKVLMVSYYFPPLGMAGVQRVTKFVKYLPEFGWQPVVLTT
                     KTIAFPHADPSLFTDVPQATPIYRAGSFDPLRLAYLWGRSFHGRPVKVGRGSRGSIGE
                     WLNLPDNKIGFVPLALAKARRIVRQEKIDLVWTTSPPPSIHGVGLALKTVFRLPWVAD
                     FRDLWSGTFDRSFPSRGHRWLAGRWQKKFVSGADAVVAVS"
     gene            complement(596..1717)
                     /locus_tag="AC16GM_07775"
     CDS             complement(596..1717)
                     /locus_tag="AC16GM_07775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2923609.1"
                     /translation="MSAPRTIAILADARSIHTQRWANYFVRRGDKVNLLSLEPGEGDA
                     YTYQRLRSRLPVRGFKYVAAVPSLRQKLSRMKPDIVNAHFVPNYGLLGLLAGFRPLVV
                     TCWGSDVLLSPLKSGLHRRRVERVIRGADFLTSDSAYLTDRLVAFGAFAEKIATVPLG
                     VEDEILALGAERHHSGDEKVVVLSTRQLEPVYDVETVLHTVPKAIMGFPHEMEFWIVG
                     AGSQRRTLETLSHAIGVSLWTKFLGWLERPRYLEVLRQAAVYVSTARSDSTSVSLLEA
                     MGAGLAPVVADIPGNREWITDGENGFLVPVDSYLSFGEKIRFLADRSELRQQFAVKNQ
                     QIIRAKARWQENMQVISELFDRLIGTKGQTFQTAASLST"
     gene            complement(1714..2706)
                     /locus_tag="AC16GM_07780"
     CDS             complement(1714..2706)
                     /locus_tag="AC16GM_07780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2923610.1"
                     /translation="MTVRQFSSATVDQGEWTRLVHSAPEATFYHTFFWTTVWEKTFPS
                     GTAHFFAVEEAGRYRSGLPAVRFSKRGLAEWHSLPFGTYGGVIGGDGATTEDRAICLE
                     AFRQASREKKSRGAILVDFNGTQPLLPGGRATALETEIVHLPAGAPWFNLWTPMARRS
                     YRKAMNLGLQIDYPHGLEDLSACFELVKLRSRRKKTAWNYPLSFYRELFQAHQNPALD
                     PEAKVLWPVARKGEKVATFQIFFQFRDQLIYWDGASLPEALPLRPNHLLFGSMFDWAS
                     RNGIQTINLGGSPGREKGLALFKRSLGGESYTYRQYRFPTALGKVVERAKKILR"
     gene            complement(2714..4972)
                     /locus_tag="AC16GM_07785"
     CDS             complement(2714..4972)
                     /locus_tag="AC16GM_07785"
                     /inference="COORDINATES: protein motif:HMM:NF019135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HIR2923611.1"
                     /translation="MRILPELEKQMLRCMLGAVVILAPFAESFAQTWLNFTNKNSIVD
                     VAAGPGVVWCATTGGALRFDLATDRIDQFTTAEGLGGTSLLSVAVDTSGTVWFGGNNG
                     TLTKYEARTNRWTVFPFIDRDNRKLKLKDIFIDGNKLWMATDVGVILFDAVRNGGEIR
                     ETYRRLGTLQQEAVNAVLVKDGRIWAAAVGGVVWAFTSDPNLLDPARWHTFTKTTGDS
                     LQGDQYQALAAFDSTVFVGSEAGLFRLVEMPADTFWQVLPVASDLIRALHAAGDSLFF
                     SGRFGLGVVTRPGLTVSFLNSDPLGTRIFPGLAMATDGRLFIATQGKGLGIYRNQTYQ
                     RRVIDGPPENFVVDVALDRRGRVWIANWNSGFAMYDGTWHTFDTLPVAPLGWAVTVDL
                     QDNVWCCSWGGGIYEFTSDGRFRAFTSANSPVRGIASTSAYTVSTDAVVDFQGNVWFT
                     NPYAYDSTALLAFRPDSSWEVFYKNARDGLVSNDIVMLYPQDDHIWIGYKSAGTAAGV
                     GDFNFNGTLGNHADDILVFYNQEREKLLSNTVTAIAIDRTGVLWVGTTAGLQRFDPDL
                     RRFIGVQMPSPLGPQVNAITIDARNNKWIGTSTGLGLITPDGQFRPPILSTNSGLVSD
                     AVAEITLDPLTGDLWIAAGSPLSGEEGGLSRFRTGFGGTVSDLKQVEAYPNPFVLESG
                     RELLTFKGLPFEATVTIYNVAGDRIWRRDRTDQWDGRNEGGELVAGGIYLFVATAPGK
                     EPGVGKVAVIRK"
     gene            complement(4972..>5416)
                     /locus_tag="AC16GM_07790"
     CDS             complement(4972..>5416)
                     /locus_tag="AC16GM_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR2923612.1"
                     /translation="ADFTSWKEFTTFAAPFGRYGCLICFESVFPDLVRRFVLSGADFL
                     VNITNDAWFGRTSGPYQHAQMVVFRAIENRISIARSANSGVSLVVDRWGRISARSPIF
                     AREVVVAPLDPAHERTFYTAHGNWFAKLASAVSLVFLGLAVFRKK"
BASE COUNT         1179 a         1689 c         1543 g         1005 t
ORIGIN      
        1 gagaccgcca ccaccgcatc ggcgccgctt acgaatttct tctgccaccg gccggccagc
       61 caccggtgtc cgcgggaggg gaaactccgg tcgaacgtac ccgaccagag atcgcggaag
      121 tcggccaccc aggggagacg aaaaactgtc ttgagagcca atccgacgcc gtggatcgaa
      181 ggcggtggcg aggtggtcca gaccagatcg attttttctt gccggacgat tcggcgtgcc
      241 ttggccaaag ccaggggcac aaagccgatt ttgttgtcgg gaagattcaa ccactccccg
      301 attgaacccc ggctccccct gccgaccttc acgggccgtc cgtgaaaact gcgcccccag
      361 agataggcca gacgcaaggg atcaaaggaa ccggcccggt aaatcggcgt tgcctgcgga
      421 acgtcggtga aaagcgacgg gtcagcgtgg ggaaaggcaa tcgtcttggt cgtcaacacc
      481 accggctgcc agccgaactc cggcaggtac ttgacgaatt tggtgacccg ctgcacgccg
      541 gccattccca gaggcgggaa gtagtagctg accatcaaga cttttttcgg gccactcatg
      601 tggaaagaga tgcagcggtc tggaaggttt gccctttggt tccgatcagg cggtcgaaga
      661 gctccgaaat cacctgcatg ttctcctgcc agcgggcctt cgcacggata atctgttggt
      721 tctttaccgc gaattgctgg cgcaactcgg atcgatcggc gaggaagcgg attttctcac
      781 caaagctgag gtagctatcg accggaacga gaaaaccatt ttcaccgtcg gtgatccact
      841 cccggttgcc ggggatgtcg gcaaccaccg gcgccaatcc cgcgcccatg gcttccaaaa
      901 gactaaccga ggtggaatcg gaacgcgcgg tggaaacata cacggcggcc tggcgaagaa
      961 cctcgagata gcggggccgt tccagccacc ccagaaactt tgtccagagc gaaacgccga
     1021 tcgcatgcga aagggtctcc agggtgcgtc tctggctccc cgcaccgaca atccagaact
     1081 ccatctcgtg cggaaagccc atgatcgctt tgggtaccgt atgcagcacc gtctcgacgt
     1141 cataaaccgg ctctaactgc cgtgtggaga gcaccacgac tttttcatca ccggagtggt
     1201 gacgctctgc tcctaaagca agaatttcgt cttcgacccc cagaggcacc gtggcaatct
     1261 tctctgcaaa agctccgaac gccaccagcc ggtcggtgag ataggcagag tcggaagtca
     1321 aaaaatcagc gccgcggatc acccgttcga cccgccgccg gtggagacca gatttgagcg
     1381 gtgaaagcag cacgtccgac ccccagcagg tcaccaccag tggccggaaa ccagcaagga
     1441 gacctaacag accgtaattt ggaacgaagt gggcattgac gatatcgggc ttcatccggg
     1501 atagcttctg cctgagagag ggcactgccg cgacgtattt gaaaccccgc accggcagcc
     1561 tcgaccgcaa gcgctgatat gtgtatgcat ccccctctcc cggttcaagc gaaagcaggt
     1621 tgaccttgtc gccccgccgg acaaagtagt tcgcccagcg ttgcgtatgg atgctccgcg
     1681 cgtccgcgag aattgctata gtgcggggag cgctcatcgc aaaatctttt tggcccgttc
     1741 cacaaccttt cccagagctg tcggaaaacg gtactgccga taggtatagc tctcccctcc
     1801 cagggagcgt ttgaatagag ccagtccttt ttcccgtccc ggcgagccgc cgaggttgat
     1861 ggtctggatg ccgttgcggc tggcccagtc aaacatcgat ccgaacaaaa gatggttggg
     1921 tcgcagtggg agtgcttccg gcagactcgc cccatcccag tagattaatt gatcccgaaa
     1981 ctgaaagaaa atctggaatg tcgcaacctt ctcacccttt ctcgccaccg gccagaggac
     2041 ttttgcttcc ggatcgagcg ccgggttctg gtgcgcctgg aacaattccc ggtagaaaga
     2101 taaaggataa ttccaggcag tctttttgcg ccggctccgc agtttcacca gttcaaagca
     2161 ggcgctgagg tcttccagtc cgtgcggata atcaatttgc agtcccaaat tcatcgcctt
     2221 ccggtaactc cgccgcgcca tgggagtcca tagattgaac cagggtgctc ccgccggcag
     2281 atgcacgatc tcggtttcga gtgctgtggc tcgcccgccg ggcaaaaggg gctgggtgcc
     2341 gttgaaatcg acaaggattg ctccgcggct tttcttctcc cggctggcct gccggaatgc
     2401 ctccaggcag atcgcccgat cctcggtcgt cgcgccgtca ccgccgatga cccccccgta
     2461 ggtgccgaac ggcagggagt gccactccgc caggcctctt ttggaaaacc gcaccgccgg
     2521 tagcccggag cggtaccgtc cggcctcttc cactgcaaaa aaatgagcgg tgccggaggg
     2581 aaaagttttt tcccatacgg tcgtccagaa aaaagtgtgg tagaaggtcg cctccggtgc
     2641 gctgtgaacc agccgggtcc actccccctg atccaccgtc gcagaagaga attggcgaac
     2701 cgtcacgtac ggatcacttg cgaatgaccg cgaccttgcc cacccccggt tctttcccgg
     2761 gagcagtggc gacgaagagg taaatgccgc ccgctaccag ttcccccccc tcgttgcggc
     2821 cgtcccactg gtcggtccgg tcccgccgcc agatccggtc gcccgcgacg ttgtaaatcg
     2881 tcaccgtcgc ctcaaacggc aatcctttga acgtcaaaag ttcgcggccc gactcaagga
     2941 cgaagggatt cgggtaggct tccacctgtt taaggtccga aaccgtgcca ccgaatccgg
     3001 tgcggaaacg cgacagtccc ccttcctcac cggagagagg cgagccggcg gcaatccaga
     3061 ggtcgcccgt aagggggtca agggtaattt ccgccaccgc gtcgctcacc agaccgctat
     3121 tggtggaaag aatgggcggt cggaactgtc cgtcaggcgt gatcaaccct aagccggtgg
     3181 aggtgccaat ccacttatta ttgcgcgcgt cgatggtgat ggcgttcacc tgcggtccta
     3241 aaggggacgg catctgtacg ccgatgaacc gccgcaaatc gggatcgaag cgctggagcc
     3301 ctgcggtggt gccgacccag agcacgccgg tccggtcgat ggcaatcgcc gtcaccgtgt
     3361 tacttaagag tttctcccgc tcttggttgt agaacacgag aatgtcgtca gcatggttgc
     3421 caagcgtccc gttaaaatta aaatcaccca cccccgctgc tgttccggcg cttttgtacc
     3481 cgatccagat gtggtcatcc tgcgggtaga gcatcacgat gtcgttggag accagcccgt
     3541 cgcgcgcatt tttgtaaaag acctcccagc tcgaatcggg gcggaaagcc agaagcgcgg
     3601 tcgaatcgta cgcatacgga ttggtgaacc acacattgcc ctgaaagtca accaccgcgt
     3661 cggtcgacac ggtgtacgct gatgtactgg ctatacctct caccggcgaa ttcgcggacg
     3721 taaaggcccg aaaccgtccg tcgctggtga attcataaat tccaccgccc caggaacagc
     3781 accagacgtt atcctggaga tcgacggtca cggcccagcc caaaggggca acgggcaggg
     3841 tatcaaaggt atgccaggtg ccatcgtaca tcgcaaaacc ggaattccag ttggcaatcc
     3901 agacgcggcc gcggcggtcc aacgccacat ccacgacaaa attctccggc ggcccgtcga
     3961 tcacccgccg ctggtaggtc tgattgcggt aaatgcccaa ccccttccct tgcgtggcga
     4021 tgaagagccg cccgtcggta gccattgcca gaccggggaa aatccgggtt ccaagagggt
     4081 cgctgttgag gaaactgacc gtaagtcctg gccgggtaac tacgcccaag ccaaagcggc
     4141 cggaaaaaaa gagggaatcc ccggcagcgt gcaaggcccg aatcaaatcg gaggccacgg
     4201 ggagcacctg ccagaaagta tccgccggca tttcaaccag acggaacagc cccgcctccg
     4261 agccgacaaa gaccgtgcta tcgaaagcgg ccagcgcctg gtattgatcc ccctgcagac
     4321 tgtcgccggt cgttttggtg aaggtgtgcc agcgggccgg atctaagaga ttgggatccg
     4381 acgtaaaggc ccagaccacc ccgccaaccg ccgccgccca gatccgcccg tctttgacca
     4441 ataccgcatt caccgcttcc tgctggagcg tacccagccg gcggtaggtc tcccggatct
     4501 cgccgccgtt gcgcaccgca tcgaacagta tcacgccgac gtcggtggcc atccagagct
     4561 tgttcccgtc gatgaagatg tccttcagtt tgagtttgcg gttatcccgg tcaatgaaag
     4621 ggaaaaccgt ccagcggttg gtgcgcgctt cgtatttcgt gagcgtgccg ttgttccccc
     4681 cgaaccagac cgtgccgctg gtatcgaccg cgaccgacaa aagtgacgtc ccccccaggc
     4741 cttccgccgt cgtgaactgg tcgatccgat ccgttgcgag atcaaagcgg agcgcaccgc
     4801 cggtggtggc gcaccagacc acgcccggcc cggccgccac gtccacaatt gaattcttat
     4861 tggtgaagtt gagccaggtc tgcgcaaaac tttcagcgaa cggcgcgaga atcacgaccg
     4921 cacccagcat gcaccggagc atctgttttt ccagttcagg caaaatccgc atcatttctt
     4981 ccgaaaaacc gccagaccca aaaatacgag actcaccgcc gatgccaact tcgcaaacca
     5041 gttgccgtgg gcggtataga acgtccgttc gtgcgccgga tcaagcgggg caacgaccac
     5101 ctctctcgcg aaaatcgggc tccgggccga gatacgtccc caccggtcga ccaccagtga
     5161 tacgccgctg ttggccgagc gggcaatgct gatccggttc tctatcgccc ggaagaccac
     5221 catttgggca tgctggtatg gcccggaggt gcggccgaac caggcgtcgt tggtaatgtt
     5281 caccaaaaaa tcggcgcccg acagaacgaa cctccgaact aaatcgggaa aaaccgactc
     5341 gaagcagatg agacagccgt atcttccaaa tggcgcggcg aaggtggtga actccttcca
     5401 gctcgtgaaa tcggcc
//
LOCUS       DBNGZD010000293         5841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1436957, whole genome shotgun sequence.
ACCESSION   DBNGZD010000293 DBNGZD010000000
VERSION     DBNGZD010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1436957"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="AC16GM_07795"
     CDS             <1..623
                     /locus_tag="AC16GM_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2923613.1"
                     /translation="VLAVGAVDSLGFHTSFSSTGPTADGRIKPDVMAQGEDDWLASAS
                     GGYGRGGGTSFATPLAAGVAALLLEIDSTLTPFQIIQRLHQSASQASRPDNLYGWGIV
                     NAARAAGFPEQPPVHRAQIRPNPVRTTATIDVTLDQPGKVELSIHTLAGERLWELVTN
                     AAGTGAQTYFWDGRNQLGQPVAGGLYLLLIKTTSFQDVVKFALTRP"
     gene            736..2343
                     /locus_tag="AC16GM_07800"
     CDS             736..2343
                     /locus_tag="AC16GM_07800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR2923614.1"
                     /translation="MESTTLTPKAGLARGCAIFKVDLKGRLVFLDDEAESLFGYSEAE
                     LYGRPLVNYLVPDDRQRLTGWLQRKRFEHPDWENCAVSIVNRQGEQIILDTIVSVHFS
                     GGVPANYQFVVRGVTIPKTEEETKSAVFLDFTSDLKGLYTAAGEGQALAQKFVATVVE
                     RFSWAWAGFFVAAEKEPLLVVQAGSPPAGFNQIGGKLKTLKGQKRVGAVAVNGSDPAE
                     FVAVAVEIGLKERGYLVAGPAQAANLRPVPREFLEHFSAVFGTLYASALQHERLEETG
                     KTLAGAEQERQHLQRLLEQKTKQMAEAADSLAHDLRAPLVAVQGFAKRLANECGSAIG
                     PQGQFSLLCLQENVEIMGKMIDDLLQFSRVTHTPEKPQPLPLREVVTDLLEEFRSTSS
                     QPVEWRGVEELPTVVFDKGKLTQVLRNLLGNAITYTEGKGHPKVTLGWTDRPEAWEIR
                     ISDNGIGIPRQYHKKIFEMFTRVPNAVTKKQPGTGMGLAIVKKVLEDHGGKIWVDSHE
                     GRGSTFHLVIPKPAAEGKLPPAEERQP"
     gene            complement(2525..3961)
                     /locus_tag="AC16GM_07805"
     CDS             complement(2525..3961)
                     /locus_tag="AC16GM_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR2923615.1"
                     /translation="MRTADETAHRPGWQTEFFAQFFDWDEPDPDLLWRFKARLDNPLI
                     KTNSDHLIGDEIPREKDPRTYRLLLLGDSSPVGLGLQSHSLAFGELLESLLEREYADR
                     KRFELINAAVSGYTSEQIARFLELRGWDFKPDLVILYCGNNDASISGSYSDRQLLGQQ
                     KLRKVRRLFSHLALYRVLRALLVQDRWSGTRPAETLQVRVPASRFGTNLTRIADQCRQ
                     HGCPLIVLKPPVPYLWPAGLQFKLFTQVSGEDGRLIIPDAMQRILGRPIKYCLSPERF
                     TALYGRGDIFTRSVYRSAYSDSLPPQEAIAYYANLIATDSGNPVLFNNLGVLLWEDRR
                     YWEADYYLKTARNLYLRHYRDSLNPTVESAGAPFLYNIGINLLSLENYGPNLLHDTAR
                     AAFQYLDSALQADYFSLRVKRTYWEKIDRLKGEKNLIVIDLPATFQAEGGEKLFIDHC
                     HPTAEGHRLIAEEILKVIRNLLSRQPTT"
     gene            4312..5664
                     /locus_tag="AC16GM_07810"
     CDS             4312..5664
                     /locus_tag="AC16GM_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HIR2923616.1"
                     /translation="MSKYPSIKTELPGPKSRALIERDERYVSHSYTRAYPTVIDRAEG
                     ALIWDVDGNCFLDFHSGIGVCSTGSCHPKVVEAIRKQLEKAVHIASADFYHERVGQLA
                     EKMSEIAPGDSDKRTFFTNSGTEAVETAFKLARYNKKKPRMIAFFGAVHGRTMGALSL
                     TNSKATQRRYFAPLVPEVTHVPFGYCYRCPLNLKYPSCNVACVDYIEGEVFTHVAPPE
                     EVAAIFVEPIQGEGGYIVPPPLWLPKLKALAEKYNILLVADEVQSGMGKTGKMFAVEH
                     WGVEPDIIAAAKALASGMPLGACIARSDLMTWERGAHSTTFGGSPVACAAALVTIDLL
                     QTGLMENAQRLGALILERLYEMQKEHWSMGDVRGKGLMIGTEFVYDRETKKPAGELAE
                     AIQMECFRNGLMLLTAGPSSIRFVPPLVIDQPMVEKALEIFEAAVTKVESRKQSAVLV
                     "
     gene            5666..>5841
                     /locus_tag="AC16GM_07815"
     CDS             5666..>5841
                     /locus_tag="AC16GM_07815"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR2923617.1"
                     /translation="MGQLVPLAAVNRGDVQESVHYGSVVVVDDTGRVIYSAGDPDFIT
                     FFRSASKPFQAIPLV"
BASE COUNT         1254 a         1616 c         1739 g         1232 t
ORIGIN      
        1 gcgttttggc ggtcggcgcg gtcgattcgc tcggcttcca cacctcattc agttccaccg
       61 gccccacggc ggatgggcgc attaagcccg acgtaatggc ccagggggaa gacgactggc
      121 tagcctcagc gagcggtggg tatggacggg gtggggggac atcttttgcc acgcctttgg
      181 cggcgggcgt ggcggccctg cttttggaaa ttgactcaac cctcacgccg tttcagatca
      241 tccaacgcct ccaccaatca gccagtcagg cctcccgacc tgataacctg tacggctggg
      301 gtattgtcaa cgctgcccgc gccgccggat ttcccgagca gccgccggtt caccgggcgc
      361 agattcgtcc aaatccagtg cgtacgacgg caaccattga cgtaactctc gaccagccgg
      421 gaaaagtcga actgtccatc cacaccctgg ccggggagcg gctctgggag ttggtgacca
      481 atgctgccgg caccggggcg caaacctact tctgggacgg cagaaaccag ctcgggcaac
      541 cggtggccgg cggtctctac ctgcttttga tcaaaaccac atcgtttcaa gacgtggtga
      601 agttcgcgtt gacccgcccg tagttttccc ctctcttttc cttgagtttc gaagccattc
      661 tgccgatgaa atggtgagag tggttcccgc ggcgagcgcg ggcccgtcag aaaacttcag
      721 accctattcg gcactgtgga gagcactacc cttaccccaa aagcaggact ggcgcggggc
      781 tgcgccatct tcaaagtcga tctcaaaggc cggctggtct ttctggacga cgaagccgag
      841 tcgcttttcg gctattccga agcggaactt tacggtcgcc cgctggtgaa ctacttagtc
      901 ccggatgacc gtcagcgcct gaccggctgg ctccagagga agcggttcga acaccccgac
      961 tgggaaaact gcgcggtttc catcgttaac cgccaggggg agcagatcat cttggacacc
     1021 attgtcagcg tccatttctc cggaggcgtg ccggccaact atcagttcgt ggtgcggggg
     1081 gtcacgatcc ccaaaaccga agaggagacc aaaagcgccg tctttttaga tttcaccagc
     1141 gacctgaaag gactttatac cgccgccggg gagggtcagg ctttggctca aaagtttgtc
     1201 gccaccgtcg tggagcgctt cagctgggcg tgggccggat tttttgtagc cgccgaaaag
     1261 gagcccctac tcgtggtgca ggccggcagt ccgccggcgg ggttcaatca aatcgggggg
     1321 aaactgaaaa cgctgaaggg ccaaaaacgg gtgggcgcag tggccgtcaa cggttcggat
     1381 ccagcagagt ttgtggcggt cgccgtcgaa atcggactga aagaacgggg gtatctggtg
     1441 gccgggccgg cccaggcggc caatctccgt ccggtgccgc gagagttttt ggagcatttc
     1501 tccgcagttt tcggtacgct gtacgcttcc gccctacagc atgagcggct ggaagaaacg
     1561 ggaaaaaccc tggccggcgc cgaacaggaa cgtcagcacc tgcagcgtct gctcgaacag
     1621 aaaacgaaac aaatggccga ggcggccgat tcgctggcgc acgacctgcg ggcgcctctg
     1681 gtcgcggtac agggattcgc caaacgtctg gccaacgagt gcggttcggc catcgggccg
     1741 caggggcagt tctccctgct ttgcctgcag gaaaatgtgg agattatggg gaagatgatt
     1801 gacgatctgt tgcagttctc ccgggtgacc catactccgg aaaagccgca gccgttgcct
     1861 ttgcgcgagg tcgtaaccga ccttttggag gagttccgtt caacctccag tcagccggtg
     1921 gagtggcgcg gcgtggagga gttgccgacg gtcgtctttg acaaaggcaa gttgacgcag
     1981 gtcctccgga accttttggg caatgccatt acctacacgg agggcaaagg ccatccgaag
     2041 gtgaccttgg ggtggaccga ccggccggaa gcttgggaaa tccgcatctc cgacaacggc
     2101 atcggcattc cccggcagta ccacaagaaa atttttgaga tgttcactcg ggttccgaat
     2161 gcggtaacca aaaagcagcc ggggacgggg atgggattgg cgattgtgaa gaaagtcttg
     2221 gaagatcacg ggggaaaaat ctgggtcgac tcccacgagg ggcgggggag caccttccac
     2281 ctcgtcattc ccaaaccggc ggccgaggga aagcttccac ctgcggaaga gcgccagccc
     2341 tgacgttctc cgttcctgtt ccggtcagtc ctttaagatc tggctataga gtgttcgacg
     2401 cggaacggct tttcgtcatc atccgcaaca tcttgaattt ttcgggaagg atctcagtga
     2461 cttcgccgaa aattcggctc cgatcttcag atgtatccca actcggcctg tgtggtttag
     2521 gaaattaggt cgtcggctgt ctactcagca ggttgcggat gaccttcaaa atctcttcgg
     2581 caatcaaccg atgtccctcc gcggtggggt ggcagtggtc gataaatagc ttttctccgc
     2641 cttcggcctg gaaggtcgcc ggtaggtcga taacgatgag attcttctct cctttcaacc
     2701 tgtcgatttt ctcccagtac gttctcttaa ctcgcaaaga gaaataatcc gcctgaaggg
     2761 cggaatcgag gtattgaaac gccgcgcggg ccgtgtcgtg caggagattg gggccatagt
     2821 tttctaacga taagagatta atgccgatgt tgtagagaaa aggagccccg gccgattcca
     2881 cggtgggatt gagactgtcg cgatagtggc ggaggtataa gtttcgagct gtcttcaagt
     2941 aataatcagc ctcccagtac cgccggtctt cccagagcaa tacccccagg ttgttgaaaa
     3001 gcaccggatt tccggagtct gtggcgatta agttcgcgta gtaagctata gcctcctgcg
     3061 gtggcagaga atcgctatag gccgaccgat agacgctgcg ggtaaaaata tcgccccgac
     3121 cgtataaagc ggtgaagcgt tccgggctca ggcagtactt gattggtctt cccagaattc
     3181 tctgcattgc atcagggata atcaggcggc cgtcttctcc ggagacctgt gtaaaaagtt
     3241 tgaactgcag tccggccggc cagaggtaag gtaccggtgg tttgagaacg atgagcgggc
     3301 agccgtgctg gcggcactgg tcggcgatgc gggtgagatt cgtcccgaac cggcttgcgg
     3361 gaactctgac ttgtagcgtt tccgccggtc gggtaccgct ccacctatcc tgcaccagga
     3421 gagcccgtaa aacccggtac agcgccagat gggagaagag cctccggact ttccgtaatt
     3481 tctgctgtcc cagtaattgc cgatccgagt agcttccgga tatcgaagca tcgttatttc
     3541 cgcagtacag tatgaccaga tcaggtttga agtcccaccc gcggagttcc agaaatcggg
     3601 cgatctgttc gctggtatag cctgaaacag cggcattgat caattcaaaa cgcttcctat
     3661 cagcatattc acgttcgagg agcgactcca acagttctcc gaaagcgagc gagtgggatt
     3721 gcagtcccag tcccaccggg gatgagtccc ccaagagcag caggcgatag gtccgtgggt
     3781 ctttttctct gggaatttcg tcgccgatga ggtggtcgga gttggttttt atcaacgggt
     3841 tatcgagacg ggccttgaat ctccagagca gatccgggtc gggctcatcc caatcgaaga
     3901 attgcgcgaa aaactccgtt tgccaccccg gacggtgcgc ggtctcgtct gcggtacgca
     3961 caacccgcgc ttccagtaac agggcggtta tctccagaag tacgagaaat agaaaagtga
     4021 taaatacggg aaaaagtagg tctcgtctca cggtattcaa tttacttggc gcgccgaatc
     4081 agtgtcaatg aaggcgaccg gtcacgcgaa acgtacagcg caagacttca ccgacctgta
     4141 accgcggttg acttttttcc gggggaagag tagtttctta gcaaggtatt tctatgttct
     4201 acgtactacg ccgcatcctg ttccgtatct ggctcaaagg gcagacctaa gcctcggttg
     4261 gcttttttcc gattgattcg aagacagagg agaccaaacc gaggagatac aatgtcgaaa
     4321 tatccgagca taaaaaccga actacccgga ccaaaaagtc gggcactgat cgaacgggac
     4381 gagcggtacg tctctcactc ttacacccgc gcctacccga cggtgattga ccgggccgag
     4441 ggcgctttga tctgggacgt ggacggcaat tgcttcctcg acttccactc gggcattgga
     4501 gtctgctcga ccggttcctg tcacccgaag gtcgtcgaag caatccgcaa gcaactcgag
     4561 aaggcagtgc atatcgcctc agccgacttc taccacgagc gggtaggcca attagcggag
     4621 aaaatgtccg agattgcgcc gggtgatagc gacaaacgga cttttttcac caactccggg
     4681 accgaagcgg tcgaaaccgc cttcaaattg gcacggtaca acaaaaaaaa gccgcggatg
     4741 atagcttttt tcggcgccgt ccacggccgg accatgggcg cgctttcgct caccaacagc
     4801 aaagcgacgc aaagacggta ctttgccccg ctcgtgccgg aggtgaccca cgtccccttc
     4861 ggttactgtt accgctgccc cttgaacttg aaatatccct cctgcaacgt ggcctgcgtt
     4921 gattacatcg aaggggaagt tttcacccac gtggcgccgc cggaagaagt ggcggctatt
     4981 ttcgtggaac cgattcaggg cgaaggcggc tacatcgtcc cgccgccgct ctggctgccg
     5041 aaattgaagg ccctggccga aaagtacaat attcttctgg tggcggatga agttcagtcc
     5101 ggcatgggca agaccgggaa gatgttcgcc gtggagcact ggggcgtgga gccggatatc
     5161 atcgccgcag ccaaggcgtt ggcctccgga atgcctttgg gcgcttgcat cgccagaagc
     5221 gatttgatga cctgggagcg cggggcacat tccaccacct ttggtgggag tcccgtggcc
     5281 tgcgcggcgg ccctggtcac cattgacctg ctccaaaccg ggttgatgga aaatgcccag
     5341 cgcttgggcg ccttgattct cgaacgcctg tacgagatgc aaaaagagca ctggtcaatg
     5401 ggagatgtgc gcggaaaagg gctgatgatc ggcaccgagt tcgtttacga ccgggagacc
     5461 aaaaagccgg ccggcgaact ggccgaggcg attcagatgg agtgcttccg caacggctta
     5521 atgcttttga ccgccgggcc ttcctccatc cgcttcgtgc cgcctttggt catcgaccag
     5581 ccgatggtgg agaaggcgct ggagattttt gaagcggcag tgacgaaagt tgaaagccga
     5641 aaacaatcgg cggtgctggt ttagaatggg tcagcttgta ccgctggccg cggtcaatcg
     5701 cggagatgtt caggaaagtg tccactatgg ctcggttgta gtggtggacg acaccggcag
     5761 agtaatttac tccgccggcg accccgactt catcaccttc ttccgctcgg cctccaagcc
     5821 gttccaagcg attccgctgg t
//
LOCUS       DBNGZD010000294         3810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_742274, whole genome shotgun sequence.
ACCESSION   DBNGZD010000294 DBNGZD010000000
VERSION     DBNGZD010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_742274"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2182)
                     /locus_tag="AC16GM_07820"
     CDS             complement(<1..2182)
                     /locus_tag="AC16GM_07820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR2923618.1"
                     /translation="MRPLHFRPKQRLSALFLDSILFFFFARAVPAQPAMIVTIEPSQK
                     DFWEGKWLGPTHGSTSLTTGPSSPVKIGLALSGGGARGFAQIGVLQVLEREGINVNCI
                     AGTSIGGVIGGLYAAGYRAAELERLAETIDWSELFTNQPKRSSLFVSQKEEADRHLIA
                     VRFDGVRPSLPVALTAGQKLSDLLASLTYRANYRSGGNFDRLPIPYRTVATDLVTGEK
                     VLFDRGDLWEAMRATIAIPLAISPLEKGQRVFVDGGLVDPVPVDVAREMGAELVIAVS
                     TVSDLAPADRLQDPIDIANQVTTVMSAHLRVEQLARADVVVAPVLGDLANVGFNRIGA
                     AIQAGREAAEQVLPWLREEIRKRKQALRDEKFSVRFVRLEGSGASSAEKFSHLLFSPY
                     PETLSREEIQAKIDSLFASGLFRTARAELHTASDTDFALVYHLEANPTMRAVYFTGNS
                     IFSEAELQACAMIPPTGDLRLHDLRQAAKSVDSLYRSRGYDLAGVQSITYDTSGAVQL
                     AIEEGRIEKITVTGNSHTRRWVVTGDFPLKAGMAFNGNLAARGVANLYASDLFERVGM
                     NLTAGRSGPVVELNVKEKKFVVLRVGGRYNDDYHTEVYGEAGHTNLFGIGNEADLVGH
                     YGSRKWLAGGRFQADQIFRTMYTYRLGFSAGIERRYRYLAHEKVATFDDRRIGITFSL
                     GRHLARLGRLSLDGKWERVQAHFLDQKPDLKYFSGTLAFRSTVDT"
     gene            2293..3081
                     /locus_tag="AC16GM_07825"
     CDS             2293..3081
                     /locus_tag="AC16GM_07825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR2923619.1"
                     /translation="MPSPKPLRELSFLATKKGRAKRKSFLVEGVRALQELARSDWPVK
                     NIFVQANWEKDVSLAALVAAFRARKIPLYPLTRRELEKITQTETPPGIAAEVAFRRFG
                     LNDLLSRSPRLLVALDGIRDPGNLGTIIRTADAFGAGGLLLSEDTAEFYSPKVVRATA
                     GSLFHLPIAVDLDLPPAFIRLKQAGYLIAVTHRQSSETAAQLSPTGRVCLVLGSEAFG
                     VRKEIASLANHHLSIPITPHVDSLNVAVAAGILLYLLSNRNISP"
     gene            3295..>3810
                     /locus_tag="AC16GM_07830"
     CDS             3295..>3810
                     /locus_tag="AC16GM_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923620.1"
                     /translation="MKKRNLCLLASLMIITIKTASFAQMPAIPPFLLPPLSPAVVADP
                     LSLSINPAGLAFTDPALLFLHANSDSTFKGDNAFFLATHQVGFGIEWLGRNFPADGKR
                     YHLGFGGKLGRGVSFGTTYSWIDADRTAYDKFFTWNFGLTYRQSRYVALALVAQNWNQ
                     HQLFNQKIPRQY"
BASE COUNT          755 a         1192 c         1095 g          768 t
ORIGIN      
        1 gcgtatccac ggtggagcgg aaggccagcg ttccggagaa gtacttgagg tcgggctttt
       61 ggtccaggaa gtgcgcctgc accctttccc acttgccatc cagggacaac cgtcccaaac
      121 gtgccaggtg ccgtcccaga gaaaaggtaa tgccgatccg gcggtcgtcg aaggtggcga
      181 ccttctcgtg ggcgaggtag cggtagcggc gctcgatccc cgccgaaaat ccgaggcggt
      241 aggtgtacat cgtgcgaaaa atctggtcgg cctggaaccg ccctccggcc agccattttc
      301 gggaaccgta gtgacccacg aggtcggcct cgttcccgat gccgaagagg ttggtgtgcc
      361 ccgcctcgcc gtacacttcc gtgtggtagt cgtcgttgta ccgcccgccc acgcgcaaaa
      421 ctacgaattt tttttccttg acatttaatt ccacaaccgg cccgctccgg ccggcggtca
      481 gattcatccc cacccgctcg aacagatcgg aggcgtacag attcgctact ccccgggcag
      541 cgagattgcc gttaaacgcc atcccggctt tgagcgggaa atcgccggtg accacccagc
      601 gccgcgtgtg actgttgccg gtgacggtga ttttttcaat ccggccctct tcgatcgcca
      661 actgcactgc gcccgacgtg tcgtaggtga tggactggac gcccgccagg tcatatcccc
      721 ggctacggta gagactatcg acgcttttgg cggcctgccg caggtcgtgc aagcggaggt
      781 cgcctgtcgg cggaatcatg gcgcacgctt gcagttctgc ttccgagaag atcgaattgc
      841 cggtaaagta cacggcgcgc atggtggggt tggcttcgag gtggtagacc agagcgaaat
      901 cggtgtcgct cgcggtgtgg agttcggccc gcgccgtgcg aaacaggccg ctggcgaaaa
      961 gtgaatcgat tttcgcctga atctcttccc gtgaaagcgt ttccggatag ggggagaaca
     1021 aaaggtggga aaatttttct gcactggacg cgccgctccc ttccagtcgg acaaagcgga
     1081 cagaaaattt ctcgtcgcgg agtgcctgct ttcttttgcg gatctcttcc cgaagccagg
     1141 gtagcacctg ctccgccgct tcgcgccccg cttggatggc tgccccaatc cggttgaaac
     1201 ccacgttcgc aaggtcaccc agcaccgggg ccaccaccac gtcggctctg gccagctgct
     1261 ccacgcgcag gtgggccgac atcaccgtgg tgacctgatt ggcgatgtcg atcggatcct
     1321 gcagccggtc cgccggcgcc aggtccgaaa cggtggagac cgcaatcacc aactctgccc
     1381 ccatctcccg cgccacatcc accggcaccg gatccaccaa gccgccgtcc acgaagaccc
     1441 gctgcccttt ctccaaagga ctgatggcca aaggaatcgc aatggtggcc cgcatcgctt
     1501 cccacaggtc gccgcggtca aagagcactt tttctccggt caccagatcg gtggcgacgg
     1561 tccggtaggg aatcggcaac cggtcaaagt ttcccccgga ccggtagttg gcccggtagg
     1621 tgagtgacgc caaaaggtcc gagagcttct gtccggcggt gagcgccacc ggcagggagg
     1681 gacgcacgcc gtcaaagcgt acggcgatta agtgccggtc cgcctcctct ttctggctga
     1741 cgaagagact ggagcgcttg ggctggttcg tgaaaagctc ggaccagtcg atcgtttccg
     1801 ccagccgctc cagttccgcc gcccggtacc ccgcggcata gagcccgccg atcacgccgc
     1861 cgatcgacgt gccggcgatg cagttcacgt tgatcccttc ccgctcgagc acttgcagca
     1921 ccccgatttg cgcaaagccg cgcgctccgc cgccggagag cgccagcccg atttttaccg
     1981 gggaggaggg acctgtggtg agcgaggtcg aaccatgggt ggggccgagc catttgccct
     2041 cccagaaatc tttctgcgag ggctcgatgg tgacgatcat ggccggctga gccggaactg
     2101 cacgggcaaa aaagaaaaat agaatagagt cgagaaaaag ggctgatagc cgctgtttcg
     2161 gtcgaaaatg taaaggccgc attgaactcg ccgtcccgcc tgcggcggga ctccggggta
     2221 aaactaccct gcccggcgga aaaggcaaag agtttgacgc gctttgtccg gaaattatgt
     2281 tggggcggcg ctatgcccag tccgaaacct ctgagagaac tctctttttt ggccaccaaa
     2341 aaagggcgcg ccaaaagaaa aagttttctg gtggaaggtg tccgcgccct gcaggaactg
     2401 gcccgctccg actggccggt aaaaaatatt ttcgtccaag cgaattggga aaaagacgta
     2461 tccctggctg ctctggtggc agcgtttcgc gcgcggaaaa ttccgctgta cccgctcacc
     2521 cggcgcgagt tggaaaaaat tacgcaaacg gaaacacctc cgggcattgc cgccgaagtc
     2581 gctttccgcc gttttggact taacgatttg ctttcccgct ctccccgtct gctggtcgcg
     2641 ctggacggca tccgcgatcc cggcaatctc ggcacgatta tccgcactgc ggatgccttt
     2701 ggcgcgggcg gactgctttt gtccgaagac accgcggagt tctattcccc caaggttgtc
     2761 cgcgccactg ccggctccct cttccacctc ccaattgccg tcgatctgga cttacctccc
     2821 gcatttattc gattgaaaca agcggggtac cttattgccg ttacccacag gcagagtagc
     2881 gagacggcgg cccagctgtc cccgacggga agagtttgcc tggtgttggg cagcgaagcg
     2941 tttggagtcc gaaaagagat tgcctcgctc gccaatcacc acctttcgat ccctattacg
     3001 ccgcacgttg attcactcaa cgttgccgtg gccgccggca tcctgttata tcttctctcc
     3061 aatagaaaca tttccccgta gcggcggttg ccaacctcgc cgacggcgag agttcttgaa
     3121 acgagggaga aaaaacgtac ggcaagttgt accggtaggg gcgactttac ttgtggtgag
     3181 ccccgctaac gacgggatcg aaccagtcgc ccgtgagctt cttgaaacga aagcgagaaa
     3241 ataccgtacg tcagagggag aacgaggaag gtacctcgca aaggagtgag cgcaatgaaa
     3301 aaacgtaatc tttgtttgct tgcgtcttta atgatcatca ccatcaaaac tgcgagcttt
     3361 gcccagatgc ccgcgatccc ccccttcctc ctgccgcccc tttccccagc ggtggtggct
     3421 gaccccttga gtctttcgat caaccccgcg gggctggcct tcaccgaccc ggcgctcctc
     3481 ttcctccacg cgaacagcga ttccaccttc aaaggggaca acgccttttt cctcgccacg
     3541 catcaggttg gctttggcat cgaatggctc gggcggaact ttcccgccga cggcaaacgc
     3601 taccatctgg gcttcggcgg caagctgggg cgcggcgtct ccttcggcac cacctactcc
     3661 tggattgatg ctgatcgtac cgcctatgac aagtttttca cctggaattt cggcctcacc
     3721 taccgccaga gccgctacgt cgccctcgct ctggtggccc agaattggaa tcagcaccaa
     3781 cttttcaatc aaaagattcc ccggcagtac
//
LOCUS       DBNGZD010000295        15422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1297321, whole genome shotgun sequence.
ACCESSION   DBNGZD010000295 DBNGZD010000000
VERSION     DBNGZD010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 15422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15422
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1297321"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1801
                     /locus_tag="AC16GM_07835"
     CDS             347..1801
                     /locus_tag="AC16GM_07835"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR2923621.1"
                     /translation="MQTLTQPAVPDLPVLPGYQVLERLGSGGMAIVYRGIQSALDRPV
                     AIKRLHTHLTPDPTFLERFAREAKAAASLHHEHIVSVIDFAQKPNGCYIVMEYVEGAT
                     LRALLRETTPLPLEIVLLILASVARGLAFAHDRGFVHRDIKPDNIMVGRDGSVKITDF
                     GIVKSNADTTLTRTGSVVGSPAYMAPELLQDLSVDSRADIFSFGVTAYEILAGAKPFR
                     GDSPSAVITSMLHHAPVPLLERNQEVSPALEDLIGRCMRKNPADRPQDLHQLAGELYE
                     FIAVFDLKDKTRILRDFMAAPIQNAVKLQGERKERHLQAAKYYLSQGLEKTMEARGEL
                     GVVLALDPAHAEAGVLLEQLNTDPKLLEALTPLPQPAHPRRQLLYPGGVVLLFVAVLL
                     LWYYVKEKSQPQASKPSGYLVIDVLPSADIYVDGKYCGSTPPPLLLNLDPGSHRVHFA
                     GENLNLKERSVTLAEGETVLLKERLKPAGNGAGE"
     gene            1821..4175
                     /locus_tag="AC16GM_07840"
     CDS             1821..4175
                     /locus_tag="AC16GM_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2923622.1"
                     /translation="MQKLIFVTVTLLLSFPATLPAFEPLFDTWMSYEVGNTPISVVAS
                     DLDGDGDQDLAVANFGFNLPDADSTVSVLMNNGNGTFSSKVDYVTGYRPYAVIAADLD
                     GDGDQDLVTANDYTTISILRNNGDGTFSTRVDYEVGLYPRSIIAADFDGDGDQDLAVG
                     NYGYFSVAILKNNGDGIFSPGVDYGYPVGPVSMYATDLDGDGDQDIAVPNAILKNDGD
                     GSFTEKVTFATGGIFTESVSASDIDGDGDQDLIAADYDNHVSVLKNNGDGTFGNPVRY
                     ETEFELRTVIASDLDNDGDQDLVTGYSNGNYLSVLKNKGDGTFAERVNYIIGCHSVSV
                     FATDLDLDSDNDLVTTNFCGYTVSVLLNHGDGTFGGTVNYGTGKYPEAVFASDLDRDG
                     DPDLVVANEGDSTISVFKNNGDGAFADRIDYVTGSSPRSVIAADLNGYGFKDLVVANA
                     LSNTVSILRNLGNGFFLSGIEYATGNHPTSVVTADLDADRDQDLVIANGGSNSVSILK
                     NLGDSNFGARTDYTTGYTPYSVVVADLDCDGNQDLVTANQNGNSVSILRNNGDGSFAP
                     RVDYNTGSEPRSVAVSDLDGDGDVDLAIANSSSAYYGIPGNTISILKNNGDGTFTPKV
                     DYQTGYRPYSIVASDFDRDGDVDLAVSSADIRHVVSVLKNKSDGTFAEKEDYGVYSGS
                     VSVFAADVDRDGAQDLAVADGYNTVSILKNLSNPGCVVRGDVDGGGSIDIADIVALIL
                     NVVSGSQLQNSIAGDTDCDGARNIVDIVLLIQYVVFGTPTPCCL"
     gene            4357..5898
                     /locus_tag="AC16GM_07845"
     CDS             4357..5898
                     /locus_tag="AC16GM_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2923623.1"
                     /translation="MRYRSLTRAMFGLALFLFLFGSAFSFDLPVQKKTLKNGMTILVL
                     ENHTAPVVSTYLRFKVGSVDEHPGITGTSHLLEHMMFKGTKDIGTANYKAELPVMEKI
                     DQTAHQIIAEQARLQNPLSDHDSTKLKALREQLHTLQAEEKKYVIKDELWETYLKNGG
                     TGLNASTSEDGTQYFVSLPANRLELWAFLESDRIANPILREFYSERDVVYEERRLRTD
                     TQPFGKLSEQFGAAAYIAHPYGWPVVGWASDLETVSREEVEKYFRRYYAPNNAIAVIV
                     GDVKAPEVFALMEKYFAPIPPQPAPDPVFTTEPPQEGERRVKAEFDANPLLLIGWHIP
                     ALGNPDWFALDVLSDILSQGRTSRLYKKIVEEKKLATNISSFLDDARYPGLFYVQATP
                     LAPHMPEEVEQAVYAEIERAKNEPVTDWELQKVKNQTDAALVRSLQSNNGLAFVLGNT
                     EAITGDWKNFITFAEKSKQVTPEQIQQVAKQYLTESNRTVGILVQVKGKKVEPPKISP
                     GSLKY"
     gene            5973..7436
                     /locus_tag="AC16GM_07850"
     CDS             5973..7436
                     /locus_tag="AC16GM_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2923624.1"
                     /translation="MRKRRTFAGLLLLAGCLIFTTGAFAQPKISEGSKKIAQFKYKDI
                     TWQIPEVGKQVDTLTLPNGIKLFLLEDHAIPIFSMNALVRTGNIYEPADQMGLAGLTG
                     TVMRTGGTETLDPDSLNRLLEFIAGSVETGIGTESGVANLNVLSKDMNLGIEIFADVL
                     MHPAFRQEKIDLAKDQIRESIRRRNDSPGQIVSREFNHLIYGAHPYGRVLEWETVKKL
                     TRDDLIAFHRKYYKPNNILLGVAGDFKKAEVVAKLKQVFADWKPEPVDFPPLPKVADQ
                     PNPGVYVIAKEINQTNVTMGHLGCDFNNPDIYAISVMNFILGGGSFTSRMTSRVRSDE
                     GLAYSVGSSFNTGSRDLGTFSATCQTKTETTHKAISLMLEEIKKIREAPVTADELKTA
                     KDSFINRYVFQFTNPGAIVGQLMGLEYFNRPRDFLKTYLDKIRAVTAADVQRAAQKYL
                     KPENLTLVVVGKTDKFDKNLDDLGQVKPIALQPPAVD"
     gene            7672..8223
                     /locus_tag="AC16GM_07855"
     CDS             7672..8223
                     /locus_tag="AC16GM_07855"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HIR2923625.1"
                     /translation="MVIIPKKIFLTKGVGKHREQLSSFELALRGAGIARFNLVSVSSI
                     FPPKCKVISKARGLQYLYDGQVVFCVIARNATNEPNRLISSSVGLAIPQDRRQFGYLS
                     EHHDYGMTEKKAADYAEDLAAEMLATTLGVGFDPNTSYDEKRQIWRISGKIVRTMNVT
                     QSAEGDKNGLWTTVVAAGIFITE"
     gene            8250..9521
                     /locus_tag="AC16GM_07860"
     CDS             8250..9521
                     /locus_tag="AC16GM_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR2923626.1"
                     /translation="MSSIFAKKQLSNLLRSKFEFQGANTQYGSHSIHSFAAKFPPQLP
                     AAFILNLTEPGEIVLDPMAGSGTALVEALLAKRRCIGIDLDPLACLIADVKTRPAGSF
                     VSEQDIDRVIFNAVAALQHTALLDKIGEKRFDEKTRLFLDYWFLRPTQHELLSLLAAI
                     DEEQNPRAKQFFKVVFSSIIVTKSGGVSLARDLAHSRPHRDSEKPRQDAFELFRKKAV
                     KALRGLRIFEKPTHDALVMRGDVRSLPLQNNVIHLIVTSPPYANAIDYMRAHKFSLVW
                     LGYPIDLLSTVRSSYIGAEKQNHHNGYYSSLANTVLKELDEVDQKRSKIVNQYFAEML
                     VALKEMYRVLTRQRYCIVVVGPSTVRGLQIPTQDILAELAEKVRFQVIDVIQRQIDRD
                     RRMLPASFARNSNSMIEQRIHEEYVLVFRKR"
     gene            9530..10336
                     /locus_tag="AC16GM_07865"
     CDS             9530..10336
                     /locus_tag="AC16GM_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK family nuclease"
                     /protein_id="HIR2923627.1"
                     /translation="MPTREETQRLAVELIQQALHEHGNTASNAWLAVYRTLLWHESVS
                     VPPHRFLPHIIDADKLRPRRGSKSKTRTIWQTRAIAIEEYLAQAIGCSVGETNLRCDQ
                     LFRRKEFEGLQRQNPLGIAFAGVIQFLLGRFGSQQIQYRNEVPADEIFPGIHLPGRSK
                     TPSIDILATHSGVPISIISAKWSLRHDRIGDITSECPAYKNAARLLRSDVKYFVVTNE
                     FDPSRLQKIITDTCVDGVVHIHKPAVTDVCRLNDRLQRLLDLTEFFERVH"
     gene            complement(10307..11110)
                     /locus_tag="AC16GM_07870"
     CDS             complement(10307..11110)
                     /locus_tag="AC16GM_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923628.1"
                     /translation="MPFTKKFFRKVRRAVVLVLPLVGLWAVGLQAQVAPDVTREVLVQ
                     FRQPGVITFNGGGTIASASYFGVTVHSTAVKAVLDQYQPELITEEGRNENLADTTRIS
                     PEGRTVRRPIFANTFRIQLPPGGNLDGLARGLDTLKAYVIFAERNGTATSNACIPATN
                     RDQLLPQQWHLRNVGQRYGGLVGADIHYIEPGTDACWVAPEATATTEVWIIDTGVEST
                     HPDFAGNATDDSDPQCLGTHGTWAAGKARRAEHCFKIGYYLMNAFKKLS"
     gene            complement(11197..12162)
                     /locus_tag="AC16GM_07875"
     CDS             complement(11197..12162)
                     /locus_tag="AC16GM_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923629.1"
                     /translation="MRRVIFLVFLTAGALASGGFFLCSKRKPTQPPPVVVEKPCEDLD
                     STLTFTCCRQDVRDPAWFPDGKTIAYANVQTDPGVHLVNPDGTNDRNILRSFLGSVHL
                     DISSDGQWILFTWGRTDQPQLDGIYKVRVTGKDTTLILIGRDNSYPRWSSDDKIIAFE
                     RETFVGDTLNGVYLMNADGTWVRQILRFGQVPDWSLDGRLVVNIGTNLYIIDTLGNIL
                     SKLTDFRADSLGRGIRVGSGRWSPGGDLILFTAYENWTDRSYLMTVRPDGTGMQKVPN
                     TLGNIFPAAWAPDNRHVVYTDGDTTKHGIYTVSILGCNKTLIAKP"
     gene            complement(12253..13287)
                     /locus_tag="AC16GM_07880"
     CDS             complement(12253..13287)
                     /locus_tag="AC16GM_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR2923630.1"
                     /translation="MRRLGFVLCLLTFSVAGVTAHTVHYDQLLDPVTHEPPIATFSIC
                     AFDPETGDFGVAVQSKFFGVGTVVPWAKAGVGAIATQAWGNTSFGPLGLEMLAHGYTA
                     QQVVDALIAGDDHRHRRQLGVIDSKGNAANWTGDSCLAWAGGIKGRYYTCQGNILTGD
                     SVVKAMARAFEATKGDLAERLMTALEAGQSKGGDSRGMQSAALLVVRDKGGYNGYTDR
                     YVDLRVDDAPNPFKEMRRLLGIQQGLKHLQVAGLKYREGKIKEAIGECREAVKTDPTN
                     ADNYYDLACYLSLDKEKTEALDALRSAMKLNPKLKKLAEGDSDLDNIRNENAFKTILE
                     GHRGTRGLRR"
     gene            13444..13866
                     /gene="ruvX"
                     /locus_tag="AC16GM_07885"
     CDS             13444..13866
                     /gene="ruvX"
                     /locus_tag="AC16GM_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR2923631.1"
                     /translation="MQRILGIDYGRKRVGVAVSDLLGLTAQMLDTLEVESESVLLEKL
                     QSLVAENGVGRIVLGLPKNMNGTPSAMTAEVQAFAGRLRQKTALPVEFIDERLTSQAA
                     LRTLHETGKKTKGNKPKLDQISAVLLLQTYLDSHPSTL"
     gene            14082..15164
                     /gene="mltG"
                     /locus_tag="AC16GM_07890"
     CDS             14082..15164
                     /gene="mltG"
                     /locus_tag="AC16GM_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR2923632.1"
                     /translation="MTFAVAKKVLALFGLAVATLLAGTALYLLFSPGPRTGGQDLAFV
                     TIEPNETAPAIAAKLADAGLIKQRRTFLWAARLLGIDRTFKTGRYKFRTGSSLWTILR
                     GFQRGEGAQMTVTIPEGSSVRQIAGLLDREVGIDSAHFVAEADSPRTILVLNLTVKRA
                     EGFLYPNTYKFYWRIDPEIAINEMLAEFQRRFDSILVARAAQVDLTVPEVVTLASLVE
                     AEAGLSSERKTIAAVFHRRLKLGMPLQCDPTVIYAIGEKRRSLSRDDLQFESPYNTYL
                     HPGLPPGPIGNPGRASVVAVLWPDTTRYLYFVARGDGSHIFSYDLEGHNRARYQIQRE
                     QDALRDSLKKAALGARIETAVKSSSP"
BASE COUNT         3568 a         4313 c         4115 g         3426 t
ORIGIN      
        1 caactacgtg gtcttcggca cccccatccc caccggggag gtctgcgggg acctgaccgg
       61 cgatggcgtc attgacatca ccgacctcat ctgtctgctc aacttcgtcg ttttcggtaa
      121 cccgataccc tgctgagaaa aagcgcacgg tcacagtaaa gagcgcccgc ttgggcgctc
      181 tttttttggg gaaaagagcc ctctttgtcc gtcgcaacca attcggtcac aaacgctttc
      241 ccgaaacttt ccaaagagtt cctcatcaat ctgcccgaaa gggcctgggc gctttcatta
      301 agtcccgctg aggggtgacg atactcgatt atgtatggat gtctctgtgc agaccttaac
      361 gcagccggct gtgccggacc tgccggtttt gccgggctac caggtgctgg aacgccttgg
      421 ctccggcggc atggctatcg tctaccgggg gattcagagc gccctcgacc gcccggtggc
      481 gataaagaga ctccacaccc atctgacccc tgatccgacc tttttggaac gcttcgcccg
      541 cgaggccaaa gcagcggctt cgctccacca cgaacacatc gtttcggtca tcgatttcgc
      601 ccagaaaccg aacggctgtt acatcgtcat ggaatatgtc gagggggcaa ccctccgggc
      661 gctgttgcgc gaaacgaccc ccctgccgct ggaaattgtg cttttgatcc tcgccagtgt
      721 ggcccgaggc ttagcctttg cccacgaccg ggggttcgtc caccgcgaca tcaagccgga
      781 taatatcatg gtgggccggg acggatcggt gaaaatcacc gatttcggca tcgtcaaatc
      841 gaacgccgat accaccctca cccgcaccgg cagcgtggtc ggcagtccgg cctacatggc
      901 gccggagctg ctgcaggacc tgtcggtgga ctcccgggcc gatatcttct cttttggcgt
      961 gacggcttac gaaattttag ccggggccaa accgttccgg ggcgattccc cctctgcggt
     1021 catcacctcg atgctgcacc acgcgccggt gccacttttg gaaagaaatc aggaggtctc
     1081 cccggcgctg gaagacttaa tcggccgctg catgcggaaa aatcctgctg atcggccaca
     1141 ggatttgcac cagctggccg gcgaactgta cgagtttatc gccgtcttcg acctcaagga
     1201 caaaaccagg atcctgcggg attttatggc cgccccgatc cagaacgccg tcaaactgca
     1261 gggtgagcgg aaggaacgcc atctgcaggc ggcgaaatac tacctgagcc aggggctgga
     1321 aaagacgatg gaagcccgcg gcgaactcgg cgtggttctg gcgctggatc cggcgcacgc
     1381 cgaagccggg gtgctgctgg agcaactgaa caccgacccc aaactgctcg aagcgttgac
     1441 tccgctcccc caaccggccc atccgcggcg gcagctgctg tacccgggcg gcgtggtgct
     1501 cctcttcgtg gcggtgctgc tcttatggta ctacgtcaag gaaaaatctc agccgcaggc
     1561 gagcaagccc tccgggtatc tggtgataga cgttcttccc tcggcggaca tctacgtcga
     1621 tggcaaatac tgcggcagca cgccgccgcc tttgcttttg aaccttgatc ccggcagcca
     1681 ccgcgtccac tttgcggggg aaaacttaaa tctcaaagaa cgctccgtca ccctggcgga
     1741 gggagaaacc gtccttctca aggaacggct gaagccggct gggaatgggg cgggagaata
     1801 gcttacatga agtggcaatg atgcaaaagt taatctttgt aacggtgact ttgctgctgt
     1861 ctttccctgc tacgctgcct gcttttgagc cactttttga cacgtggatg agctatgaag
     1921 taggaaatac gcctatatcg gttgttgcct cggatttgga tggcgatgga gaccaagatt
     1981 tggccgtggc aaattttggg ttcaatcttc ctgatgcgga cagcactgtt tcggtgttaa
     2041 tgaacaatgg aaacggtaca ttttcctcca aagttgatta cgttaccggg taccgacctt
     2101 atgcggttat tgctgcagat ttagatggcg atggagatca ggatttggtg acggcgaatg
     2161 attacacaac catttcgatc ttaaggaaca atggagacgg aactttctcc acaagagtcg
     2221 actacgaagt gggtttgtat cctcgctcga ttatagctgc cgatttcgat ggagatggag
     2281 atcaagattt ggcagtgggc aattacggat atttctccgt tgcaatctta aagaataatg
     2341 gagatggaat tttctcccct ggagtggact acggctaccc ggtaggcccg gtctcaatgt
     2401 atgccaccga tctggacggc gatggagacc aagatattgc ggtaccaaac gcaatcttaa
     2461 aaaacgacgg tgatggttct tttacggaga aagtcacctt cgcaacgggc ggcatcttca
     2521 ccgagtctgt ctctgcttct gatatagacg gtgacgggga tcaagatttg atagcggcag
     2581 attacgacaa tcatgtttca gtgttaaaaa ataatggaga tgggactttt ggtaatccgg
     2641 tacgctatga aacggaattc gaacttcgca ctgtcattgc ttctgattta gacaacgatg
     2701 gagatcagga tttggtgaca ggctattcta atggcaatta cctatcggtc ttaaaaaaca
     2761 aaggagacgg gacctttgct gaaagagtca actacattat tggatgccat tcggtatcgg
     2821 ttttcgcaac agacttagac ttggacagtg acaacgactt agtaacaacg aatttttgcg
     2881 gatacacggt ttcagtactt ctgaatcacg gggacggaac gtttggaggc acggttaatt
     2941 acgggacggg gaaataccct gaagcggttt tcgcttccga ccttgaccgg gatggcgatc
     3001 cagacctggt agtggcgaac gaaggagaca gcaccatttc tgtatttaag aacaacggag
     3061 acggcgcttt cgccgatagg atagattacg taacgggaag cagtccgcgc tcagtcatcg
     3121 ctgctgacct gaacggatac ggatttaagg acttggtagt ggccaacgca cttagcaata
     3181 ccgtatcaat tttgagaaac cttgggaacg gtttttttct ttctggaatt gaatatgcaa
     3241 cgggaaacca cccaacatcc gtagtcacag ctgatcttga tgcggataga gatcaggatt
     3301 tagtaatagc gaacgggggg agcaattcag tttcgatttt gaagaacctc ggtgatagta
     3361 actttggtgc cagaaccgac tatacgacgg gatatactcc ttactcagtc gttgttgcag
     3421 atctcgactg tgatggcaac caagacttgg taacggcgaa ccaaaacggc aactcggttt
     3481 ccatcttaag aaataacggg gacggctctt ttgccccccg ggttgactac aacacaggga
     3541 gtgaaccccg ttcagtcgcc gtgtccgatc tcgatggaga cggagacgtg gacctagcta
     3601 ttgcgaattc aagttcggcc tactacggaa ttcccggaaa tacaatttca attttgaaga
     3661 acaacggaga tggaacgttt acaccaaaag ttgattacca aactggctac cggccctatt
     3721 ctatcgttgc ttcggatttc gacagggatg gagatgtaga tttagctgtg tcgagcgcag
     3781 atatccgaca cgttgtttca gtactaaaga ataaaagcga cggaacgttt gctgagaaag
     3841 aagattatgg agtttacagc ggttcagtct cggttttcgc agcggatgta gaccgagacg
     3901 gtgctcagga tttagcggtg gccgatggat acaacacggt ttctattttg aaaaatttgt
     3961 ccaaccccgg ctgcgttgta cggggtgatg tagacggcgg aggctcaatc gatattgcgg
     4021 atattgtagc cttgattctg aatgtagtgt ctggaagtca attacagaat tccattgctg
     4081 gagataccga ctgcgacggg gcgcggaaca tcgtggacat cgtgttgctc attcagtacg
     4141 tggtgtttgg aaccccaacg ccctgttgcc tgtaaggtag cagacccctc cctgccctcc
     4201 cctcaaaggg gagggaactt actggcaaaa cttctataaa cccgcgttga caaatcgggg
     4261 cggaaacgct actttccacc cgtgctgtta accgaccacg tcccatctca gaagcactca
     4321 cctttcccga aaaattgggt aaaaaaggag gttcagatgc ggtaccgctc gttaacgcgg
     4381 gcaatgttcg gtttggcgtt gttcctgttc cttttcggat cggccttcag tttcgacctg
     4441 ccggtccaga aaaagacgct caaaaacggg atgaccatct tggtgctgga gaaccatacc
     4501 gcaccggtgg tttccaccta cctgcgcttc aaagtcggct cagtggacga gcaccccggc
     4561 atcaccggca cctcgcacct gctcgaacat atgatgttca agggcaccaa agatatcggc
     4621 acggccaact acaaggccga actgcccgtg atggaaaaaa tcgaccagac ggcgcaccag
     4681 atcattgccg agcaggcccg gctccagaat ccgctttccg atcacgattc gaccaaactc
     4741 aaagccctgc gtgaacagct gcacaccctc caggccgaag agaaaaagta cgtgatcaaa
     4801 gacgaactct gggaaaccta cctgaagaac ggcggcaccg ggctgaacgc ctccacgagc
     4861 gaagacggca cccagtactt cgtttccctg ccggccaacc ggctggaact ctgggccttt
     4921 ctggaatccg accggatcgc caacccgatt ttgcgggaat tctactccga acgcgacgtg
     4981 gtctacgagg aacggcggct ccggaccgac acgcagccgt tcgggaagtt atccgagcag
     5041 ttcggcgcgg cggcctatat cgcgcatccc tacggctggc cggtggtggg ctgggcctcg
     5101 gacttagaaa ccgtttcccg cgaggaagtg gagaagtact tccggcgcta ctacgccccc
     5161 aacaatgcca tcgccgtaat cgtgggcgac gtgaaagccc ccgaagtatt cgccctgatg
     5221 gagaaatatt tcgcccccat tccgccccag cccgcgcccg acccggtctt taccaccgag
     5281 cccccccagg aaggcgagcg ccgggtgaag gcggagttcg acgccaaccc cctgctcttg
     5341 atcggctggc acattccggc tctggggaat ccggactggt tcgccttgga cgtcctctcc
     5401 gacattttgt cccagggacg caccagccgc ctctacaaaa agatcgtgga agaaaagaaa
     5461 ctggcaacca acatctcctc tttcctcgat gacgcgcgct accccgggtt attctacgtg
     5521 caggccacgc ccctggcccc gcatatgccg gaagaagtgg aacaggcggt ctacgcagaa
     5581 atcgagcggg cgaaaaacga gccggtcacc gactgggaat tgcagaaggt gaaaaaccag
     5641 accgatgccg ccctggtgcg cagtctccag tcgaacaacg gcctggcctt cgttctgggc
     5701 aacaccgaag ccataaccgg ggattggaag aactttatta ccttcgcgga gaaatccaaa
     5761 caggtgaccc ccgagcaaat ccagcaggtg gccaaacagt atttgaccga gtcgaaccgt
     5821 acggtgggga tcctggtaca ggtgaagggg aagaaagtcg aaccgcccaa aatcagcccg
     5881 ggctcgctca agtattaacc ttgcgctgac cgagaagaac cgggaacatt ttgaaaactc
     5941 ataacctgag ttcggaaaaa ggaggatcga tcgtgaggaa acgtcgcaca tttgcgggcc
     6001 tattgctcct ggccggttgc ctgattttta cgaccggtgc ttttgcccag ccgaaaatct
     6061 ctgagggcag caaaaaaatc gcgcagttca agtacaaaga catcacctgg caaataccgg
     6121 aggtgggcaa gcaggtcgac accctgaccc tgcccaacgg gatcaaactc ttcctcttgg
     6181 aagaccacgc catcccgatt ttttcgatga acgccctcgt ccgcaccggc aacatttacg
     6241 aaccggcgga ccaaatgggg ctggccggcc tcaccggcac ggtgatgcgc accggcggca
     6301 ccgaaacgct cgacccggat agtctcaacc ggcttctgga gttcattgcc ggctcggtgg
     6361 agaccggcat cggcactgaa tcgggggtcg ctaacttgaa cgttttgtcc aaggacatga
     6421 acttagggat tgagattttc gccgacgtat tgatgcaccc ggctttccgg caggaaaaaa
     6481 tcgacttggc caaagaccag atcagagaat cgatccggcg gcgcaacgac tcgccggggc
     6541 agatcgtttc ccgcgagttc aaccacctga tttacggagc acacccctac ggccgcgtgt
     6601 tggagtggga gacggtcaag aaactgaccc gggatgacct gatcgccttc caccggaaat
     6661 attacaaacc gaacaacatt cttttgggtg ttgccggaga tttcaaaaaa gccgaggtgg
     6721 tagcgaagtt gaaacaggtc tttgccgact ggaaaccgga accggtggac ttcccccccc
     6781 tgccgaaagt ggccgaccag ccgaaccccg gcgtctacgt catcgccaaa gaaatcaacc
     6841 agacgaacgt gaccatgggc cacctggggt gcgacttcaa taatcccgac atttacgcca
     6901 tatcggtgat gaacttcatc ctgggcggcg gctcgttcac ctcgcggatg acctcccggg
     6961 tgcgctccga cgaaggattg gcctactcgg tcggctccag cttcaacacc ggctcccgcg
     7021 atctgggcac cttctccgcc acctgccaga ccaaaaccga aacgacgcac aaagccatct
     7081 ccctgatgct ggaggaaatc aaaaaaatcc gggaggcgcc ggtcaccgcc gacgaactga
     7141 aaacggccaa agattccttc atcaaccgct acgtctttca gttcaccaac ccgggagcga
     7201 tcgtcggcca gttgatgggg ttggagtact ttaaccgccc gcgggacttt ttgaaaacgt
     7261 acctggacaa gatacgcgcc gtcaccgcgg ccgacgtcca gcgcgcggcc cagaagtacc
     7321 tgaagccgga gaacttgacc cttgtcgtgg tggggaagac ggataagttt gacaagaatc
     7381 tcgacgacct gggccaggtg aagccgatcg ccctgcaacc cccggccgtg gactgattgc
     7441 ggatcgaaac gcaagagtcc cgccttaggc ggcggagatc ggggttgtct tttctccgcc
     7501 ggccttccta ggtttcttct ccgagctccg gcccgcggcg cgccgacacc atcacggcat
     7561 caaaaaaacc tgcttgaccg aagcaatttt tattgcgtcc cggcgcacag actgtatatt
     7621 ttttcccgat tttgaaaacg ggcgggtcga aaaaagcgga ggcatggtag cgtggtcatc
     7681 attcccaaaa agattttcct caccaaaggg gtcggcaagc accgggagca gctctcgtcg
     7741 tttgagctgg ccctgcgggg cgccggcatc gcccgtttca acctggtttc ggtctccagc
     7801 attttcccgc ccaagtgcaa ggtcatctcc aaagcgcgcg gcctgcagta cctttacgac
     7861 ggccaggtgg tcttctgcgt catcgcccgc aacgccacca acgagccgaa ccggctcatc
     7921 tcctcctcgg tggggttggc cattccccag gaccggcggc agttcggcta cctttcggag
     7981 caccacgact acggcatgac cgaaaagaaa gccgccgact acgccgaaga cttggccgcc
     8041 gagatgctcg ccacgactct gggcgtcggt ttcgacccga atacgtcgta tgacgagaag
     8101 cgccagatct ggcggatctc tggcaagatc gtgcggacga tgaacgtcac ccagtcggcg
     8161 gagggggaca aaaacggcct ctggaccacc gtggtggcgg cggggatttt tattacggag
     8221 taactaaatt acaaagaagg gattctaact tgagttcaat ttttgcaaaa aaacaactct
     8281 caaatttgct ccgtagcaaa ttcgaattcc agggggctaa tacccagtat gggtcgcaca
     8341 gtatccattc gtttgcggca aaatttccac ctcagttacc agcagccttc atactgaact
     8401 taactgaacc cggtgaaatc gtacttgatc caatggctgg ttcggggacg gcactggttg
     8461 aagcccttct ggccaagcgt aggtgcatcg gaatagacct tgaccctctg gcttgcctta
     8521 ttgccgacgt aaaaacaaga ccagctggct cattcgtctc tgagcaggat attgatcgtg
     8581 taatttttaa cgctgtagcg gctttacagc acacagcctt gctggataaa ataggtgaaa
     8641 aacgttttga tgagaaaacc cgcttatttc tcgactactg gtttcttaga cccacccaac
     8701 acgaactttt aagtcttttg gctgccatag atgaggagca gaacccacgt gcgaaacagt
     8761 tttttaaagt ggttttttcc tcaattatcg ttaccaagtc cggaggcgtc tcattagctc
     8821 gcgacctcgc tcattcacgc ccacatcgtg atagcgaaaa accccgccaa gatgccttcg
     8881 agctcttccg aaaaaaggcg gtaaaggccc tacgtggcct gcgtattttt gaaaaaccta
     8941 ctcacgatgc tctcgtgatg cgaggtgatg tccgttcatt acctctacag aataacgtta
     9001 ttcatctcat agtaacttca cctccgtacg ccaatgccat tgattatatg cgtgcacata
     9061 aatttagcct tgtctggctg gggtacccta tcgatctcct ctctactgtc cgcagttcat
     9121 acattggcgc cgagaaacaa aatcaccaca acggctatta ttcctcgttg gcgaataccg
     9181 ttctgaaaga actggatgaa gtagatcaga aaagaagtaa aatagtaaat caatatttcg
     9241 cagaaatgct tgtggcctta aaggaaatgt atcgtgtcct cactcggcaa aggtactgta
     9301 tcgtcgtggt cggaccctct actgtacggg ggcttcaaat accgacccaa gacattttag
     9361 ccgaattagc tgaaaaagta cgttttcagg ttatagatgt cattcaacgt caaatagacc
     9421 gtgatcgacg aatgcttcct gctagttttg cccggaacag taattcaatg atagaacaac
     9481 gcatccacga agagtacgtc ctggttttcc gcaaacggta gttgcgccgt tgcccacccg
     9541 agaagagaca cagagactcg cggttgaatt aattcagcaa gctctacatg agcacggaaa
     9601 tactgcttcc aatgcttggc ttgcggttta tcgcacgttg ctctggcatg agtcagtcag
     9661 cgttcccccg cataggtttc taccccacat cattgatgct gacaaacttc gtcctcgacg
     9721 cggttccaag agcaagacca gaaccatctg gcagaccagg gctatagcta tagaagagta
     9781 tttagcacag gctataggtt gttcggttgg tgaaacgaat ttgcgctgtg accaactttt
     9841 tcgccgtaag gagttcgagg gattacaaag gcaaaatcct cttggcattg ccttcgccgg
     9901 agtcatacag ttcctcttag gacgtttcgg gtcccagcag atccaatatc gaaatgaggt
     9961 tcctgccgat gaaatctttc ctggaattca cttaccgggg cggagcaaaa ctccctcgat
    10021 tgacattctg gccacccact ccggtgtgcc catttctata atctcagcaa aatggagtct
    10081 gcggcatgac cggatcggtg atattaccag cgagtgtccc gcatataaaa atgcggctcg
    10141 acttctgaga agcgacgtaa agtatttcgt cgtcacaaat gaatttgacc cctcccgcct
    10201 gcaaaagatc ataaccgaca cctgcgttga tggcgttgtc catatccata aaccagcagt
    10261 taccgacgtt tgcaggctga atgaccgact tcagcgactg ctcgacttaa ctgagttttt
    10321 tgaacgcgtt cattaagtag taacctattt tgaaacagtg ctcagcgcgc cgcgccttgc
    10381 ctgccgccca ggtgccgtgt gtgcccaaac attgcgggtc tgaatcgtcc gttgcgtttc
    10441 cagcaaaatc gggatgagtg ctctcgacgc cggtgtctat aatccatact tcagtagtag
    10501 cagtcgcttc cggcgccacc cagcaggcgt cggtgcccgg ttcgatgtaa tggatgtccg
    10561 ctccaaccag gccaccgtac ctttgaccaa cgttccgtaa gtgccattgc tgcggcagca
    10621 actggtctct attcgttgcc gggatacagg cgttggaggt tgcagtgccg ttgcgctcgg
    10681 caaaaatgac gtaggctttc agcgtatcca gcccacgggc gagtccatcc agattaccgc
    10741 ctggcggcaa ctggatacgg aaggtgttgg cgaaaatcgg ccggcgaaca gttctacctt
    10801 ccggggaaat gcgggtcgta tcggctaaat tttcattcct cccttcctca gtaataagtt
    10861 ccggctggta ctggtctaaa accgctttta ccgccgtact gtgaactgta actccgaagt
    10921 acgaagcgga agcgatggta ccaccgccgt tgaacgtgat gactccgggt tggcggaatt
    10981 gcactaacac ctcacgggtt acgtctggcg caacctgagc ttgcagcccg accgcccaga
    11041 ggccgaccag cggcaacacc agaactactg cgcggcggac tttccgaaaa aacttcttgg
    11101 tgaacggcat tgctccctcc ctggtaaaat ggttggcgct cctgttcctt gatcccgcct
    11161 cggcgggact cgagactgtg aacttgtcga accgtgctac ggttttgcga tcaacgtttt
    11221 attgcatcct aagattgaaa ccgtataaat cccatgctta gtcgtgtccc cgtcagtgta
    11281 gactacgtgc ctgttgtcag gtgcccaagc agccgggaaa atgttgccga gcgtatttgg
    11341 tactttctgc atacccgtcc cgtcaggccg aacggtcatc agataggacc ggtcggtcca
    11401 gttctcgtac gcggtaaata agattaaatc gcctccgggc gaccagcggc cggaacctac
    11461 tcgtattccc cgtcccagag agtcggcgcg aaaatcggtc aacttagaga ggatatttcc
    11521 caaagtatca ataatgtaca agtttgtacc tatgtttacc accagccgcc cgtccagact
    11581 ccagtctgga acctggccga aacgcaagat ttgccgcacc cacgtgccat cggcgttcat
    11641 caggtaaacc ccgttgaggg tatcccccac aaaggtctca cgctcaaagg caattatttt
    11701 atcatccgac gaccatcggg gataagaatt gtcccggcca atcaaaatta aggtcgtatc
    11761 tttgcccgtc acccgaacct tatatatccc gtccaactgc ggctgatcgg ttcgtcccca
    11821 tgtgaagagg atccactgtc cgtcgctgga aatatccaaa tggaccgatc ccagaaatga
    11881 ccgcaagata ttgcggtcat tcgtgccgtc cggattgacg agatgaacgc cggggtccgt
    11941 ttgtacattt gcgtaagcaa tcgtttttcc atccgggaac caggccgggt cgcggacatc
    12001 ttgccgacag caggtgaaag tgagggtgga atccagatcc tcacagggct tttccaccac
    12061 caccggcggg ggctgggtgg gcttcctctt gctgcaaaga aagaaaccgc cggaggccaa
    12121 tgctcctgcg gttaaaaaaa caagaaagat tacccgtcgc atttcaaacc ctccctcggg
    12181 gaggcagtcc ttgataaatt tataagaaag tcattaattt gtcaagtctt ttcgtctcta
    12241 tagccgctgt tttcaacgtc gcaaaccgcg cgttccccga tgtccttcca ggattgtttt
    12301 gaacgcgttc tcgttgcgaa tgttgtccag atcggaatct ccctcggcca gtttcttcaa
    12361 cttcgggttc agtttcatcg ccgagcgcag ggcatctaac gcctcggttt tttccttgtc
    12421 gagcgacagg tagcaggcta agtcgtaata gttgtcggcg ttggtggggt cggttttgac
    12481 cgcctctcga cactcgccga tcgcctcttt gatttttccc tcgcggtact tcaatcccgc
    12541 cacctgcagg tgttttaacc cctgctggat gccgagcagg cggcgcattt ctttgaaggg
    12601 gttgggggcg tcatcgaccc gcagatcaac gtagcggtcg gtgtagccgt tgtagccgcc
    12661 tttgtcgcgc acgactaaga gcgcagccga ctgcatcccg cgcgagtcgc cgcctttgga
    12721 ttggcctgct tccaaagcag tcatcaggcg ttcagccaga tcacctttgg ttgcttcaaa
    12781 agcccgagcc attgccttca ccacgctgtc tcccgtaagg atattcccct gacaggtgta
    12841 gtagcgccct ttgatgcctc ccgcccaggc caggcaggaa tcgcccgtcc agttggcggc
    12901 attgcctttc gagtcgatga cccctaattg ccggcggtgg cggtggtcat ctcccgcgat
    12961 gagtgcatcc accacctgct gggcggtgta gccgtgggcc agcatctcca gccccaaagg
    13021 gccaaaggaa gtattgcccc aggcctgggt ggctatggct cccaccccgg ctttggccca
    13081 ggggacgacc gtcccgaccc cgaagaattt tgactgcacc gccacgccaa aatcaccggt
    13141 ttccggatcg aaggcgcaaa tggaaaaagt ggcgatgggc ggttcatgag tcaccgggtc
    13201 gaggagttga tcgtagtgaa cggtatgtgc cgtgacaccg gcgacagaaa atgtcaacag
    13261 acacagcaca aatcccaggc gccgcataga aatctccttt gttggaaaag ttgcaaacat
    13321 gtggcctaag ttaccctctt tgagagaaat ggcaagggta aagcctgcgg gggaaaacgg
    13381 ccgcaacgtt tgacggaggc agcctttgag aataaattat cggtggcacg tctgtaaatc
    13441 cctatgcaga gaattttagg catcgattat ggcagaaagc gagtgggcgt agcggtctcc
    13501 gatcttttgg gcttaacggc gcagatgctg gacaccttgg aggttgaatc ggagtcagtc
    13561 cttttggaga aactacagtc tctggtggct gaaaatggtg tcgggcgcat cgtgctcggc
    13621 ctcccgaaaa atatgaacgg cacgcccagc gcgatgaccg cggaagtcca ggcctttgcc
    13681 ggccggctgc gccagaaaac cgccctaccg gtcgaattca tcgacgagcg gctcacctcc
    13741 caggccgctc tgcggacgct gcacgaaacg gggaaaaaga cgaaagggaa caagccgaaa
    13801 ctcgatcaga tcagtgccgt actgttgctc cagacctacc tcgacagcca cccctccacc
    13861 ctttaagtac tcatggccgc accgccacct cctgtttctc ccgtcgggat aaactccgac
    13921 accccgacac ctcgacaccg acttttcctt gactggctga tctggggcat ctcaactttt
    13981 ttgatttttc tgtcgagcat actactggcg gtggggatac tggtggtgct ggtgctgcgt
    14041 cagataaacc gccttttccg atcccgaccg cctgaggcgt tatgactttt gccgtagcca
    14101 aaaaagtcct ggctctcttc ggccttgcgg tggcgacttt gcttgcgggc accgccctgt
    14161 atctcctgtt ctctcccggc ccgcgtaccg gcggccaaga tttggctttt gtcactattg
    14221 agcccaatga aaccgctccg gccatagccg ccaagctcgc cgacgcgggg ctgatcaaac
    14281 agcggcgcac cttcctctgg gcagcgcggc ttttggggat tgaccgcacc ttcaaaaccg
    14341 gccgctacaa gttccgcacc ggttcgtccc tctggacgat tctccgggga tttcagcgcg
    14401 gtgaaggggc ccagatgacg gtgacgattc ccgaaggctc tagcgtccgg cagattgcgg
    14461 gacttctgga tcgcgaagtg ggcatcgact cggcccactt tgtggctgag gccgattcgc
    14521 cgcgcactat tctggtgctc aacctaacgg tcaaacgggc cgagggattc ctctacccca
    14581 acacttacaa gttctattgg cggatcgatc cggaaatcgc catcaatgag atgctggccg
    14641 aattccagag gcggttcgat tcgatcctgg tggcgcgcgc cgcgcaggtg gacctgaccg
    14701 ttcccgaagt ggtgaccctg gcctccctgg tggaagccga agccggtctg tccagcgagc
    14761 ggaaaaccat cgcggcggtc ttccaccggc ggctgaaact gggaatgcct ttgcagtgcg
    14821 accccacggt gatctacgcc atcggcgaaa aaaggcgctc actttcccgt gacgatttgc
    14881 agttcgaatc gccctacaac acctacctgc acccgggcct gccgccgggg ccgattggca
    14941 accccggccg tgcttcggtc gtggcggtcc tctggcccga tacgaccagg tacctctact
    15001 tcgtcgccag aggcgacggc agccacatct tctcgtacga cctggagggc cacaaccggg
    15061 ccagatacca gattcagcgg gagcaggatg cgctgcgcga ttcgctaaag aaagcggcct
    15121 tgggggccag aattgaaacc gccgtcaaat cgtcttcgcc gtaactcccg gcacggcctt
    15181 tatccgtccc agtcccagta gatgcgccga gagagatccg gtttgcctaa ataatcttta
    15241 acttttgata atctctgtta agatgatttg cccgaaattt taagcgctaa ttctaaaggg
    15301 taaagtccca ttccagccac caaattgata tgttggtggc atggcaagac gactgaggtg
    15361 tccacgctgc gacagtggca agtactggat tctccgaggg agaaaacgcc ggtgtgcaca
    15421 ct
//
LOCUS       DBNGZD010000296         3590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1714681, whole genome shotgun sequence.
ACCESSION   DBNGZD010000296 DBNGZD010000000
VERSION     DBNGZD010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1714681"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="AC16GM_07895"
     CDS             complement(<1..780)
                     /locus_tag="AC16GM_07895"
                     /inference="COORDINATES: protein motif:HMM:NF052691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR2923633.1"
                     /translation="MNRFFSLLLAVVLLPLSYPSAQDFWQPSNGPYGGTVLSLALNSS
                     GHIFAGTNDGGVFRSTDNGANWTQTDLNIATVYSLALNSSGHIFAGTDAGGVFRSTDN
                     GANWTPVNTGLTYPYVRSLAINSSGHIFAGTSGGGVFRSTDNGASWTPVNTGLTYPYV
                     LSLALNSSGDVFAGTYGGVFRSTDKGANWTQTGLTNTTVLSLALNSSGHIFAGTFGGG
                     VGVFRSTDNGASWTPVNTDLMNTTVQSLALNSSGHIVAGTYV"
     gene            complement(902..2272)
                     /locus_tag="AC16GM_07900"
     CDS             complement(902..2272)
                     /locus_tag="AC16GM_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923634.1"
                     /translation="MHISPTFREKWKKCWLDPVYFSEAYLGVNLHEGQKRWLSNSTQA
                     ENVLHAGNRWGKSLIQAVKFLHRLIFQIRRDPKITDQRYTAVNVSITQNQANLVFNHV
                     LRLIRQRPGLGHLIADLRFTPFPQLELKNGSLFSARSSQRRGEYLLGEDYDYVNFDEV
                     AFEPHPDYVVQEVLMLRLADRNGTLDYTSTPKGKNWFYRKCMELERNPNRGYAQTGDS
                     RENPFISQEYLQAKLTTLSPARVLQNIGGQFVEDDNSIVKQDEISAALERSTGLKPPQ
                     PDRLGESSLRRYTHGWDLARKRSFTVGITLDVTEKPYQLVAFERFHERPWAAVYAAIR
                     ERQKTYGGQTVIDSTGLGDLVLEELKDIKAQGFNFGAQGGKAKFDLLLNLKRAHEENL
                     IAYPYLEQIDDSGELWTLPNELSELTWEDNQHCDAAMALALALWGARRKGSEEIFLPF
                     RVGLWK"
     gene            complement(2533..>3590)
                     /locus_tag="AC16GM_07905"
     CDS             complement(2533..>3590)
                     /locus_tag="AC16GM_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923635.1"
                     /translation="NSAIWTWTRLCTSPNRAEVKTDNAALKTRAEEILVDLDQRIFEH
                     DFAKFSGLDMLLAQFFTGLFTDGAVCGEVVLSPAGDRIDRFYFIDPATIEFIPNRNGQ
                     LELYQVTEDRPIRLNPHSTFYFGLDTDVANPRGRSLLSGIPFVTRVEQQLVADMQKTM
                     HNAGYHRLHVKIKPPEKKDAESDDAYVARANRYFEDTVGMMKKIAPEDNPVTWNEVEI
                     AYIGPTGQYAGASGWYLNHKAMVEDIASGTHLDPFMLGYSYGTTQHWAGFKLELVERQ
                     VISVQRPAKRFVEWLHNLELALHGLDLRVEHHFDNRRTFGLLEARRAEQMHLDNVLKQ
                     QRAGFLTFDEAKQRAFE"
BASE COUNT          770 a          969 c         1069 g          782 t
ORIGIN      
        1 gacatacgtc ccggcaacga tgtgcccgct cgaattgagg gccagagact ggacagtagt
       61 attcatcagg tcggtgttga ctggtgtcca gctggcgccg ttatcggtgg agcgaaaaac
      121 gccaacgccg ccgccaaaag tcccggcaaa gatgtgcccg ctcgaattga gggccagaga
      181 caggacagtg gtattcgtca agccggtttg agtccagttg gcgcctttgt ccgtggagcg
      241 aaaaacgccg ccataagtcc cggcaaagac atcgccgctc gaattgaggg ccagagacag
      301 gacataagga tacgtcaggc cggtgttgac tggtgtccag ctggcgccgt tgtcggtgga
      361 gcgaaaaacg ccgccgccag aagtcccggc aaagatatgc ccgctggaat tgatggccag
      421 agaacggaca taaggatacg tcaagccggt gttgactggc gtccagttgg cgccgttgtc
      481 ggtggagcga aaaacgccgc cggcatcagt cccggcaaag atgtgcccgc tcgaattgag
      541 ggccagagaa tagacagtgg caatattcaa gtcggtttga gtccagttgg cgccgttgtc
      601 ggtggagcga aaaacgccgc cgtcattagt cccggcaaag atgtgcccgc tcgaattgag
      661 ggccagagaa aggacagttc cgccgtaagg gccgttgctg ggctgccaaa aatcctgggc
      721 gcttgggtat gatagaggca acaggacgac cgccagcaga agagaaaaga aacggttcat
      781 ttttgcccct ccttcagggc gataaaaata agccctcttt atcttatttg tcaagtcatt
      841 tttgggaagg gtaaacagct tgagtggccc aagggtttag gtcaattttc aagaagctga
      901 atcacttcca caaccccacc cgaaacggca gaaaaatctc ctctgaccct tttcgccgcg
      961 cgccccacag agccaatgcc agtgccatcg cggcgtcgca gtgctggttg tcctcccagg
     1021 tcagttcgga gagttcgttg ggcagggtcc agagttcgcc ggagtcgtca atctgttcca
     1081 gatacgggta ggcgatgagg ttttcctcgt gcgcccgctt gaggttcaag agaagatcga
     1141 acttggcctt cccgccctgc gcgccaaagt tgaacccctg cgctttgatg tctttcaatt
     1201 cttccaacac cagatcgccc aagccggtgg agtcgatcac ggtctggccg ccgtaggttt
     1261 tctgccgttc ccggatcgcg gcgtagaccg ccgcccaggg gcgctcgtgg aagcgctcaa
     1321 aggcgactaa ttgatacggc ttctcggtca cgtcgagcgt gatgccgacg gtgaaactcc
     1381 gcttgcgggc taaatcccag ccgtgggtgt agcgtcgaag actcgattct ccgaggcggt
     1441 cgggttgcgg tggtttcaaa cctgtagagc gttccagcgc ggcggagatt tcgtcctgct
     1501 tcacaatcga gttgtcatcc tccacaaatt gaccgccgat gttctgcaga acccgcgcgg
     1561 gggagagggt ggtcaatttc gcctgcaaat attcctggct gatgaaggga ttctcccggc
     1621 tgtcgccggt ctgcgcgtat ccccggttgg gattccgctc caactccatg catttgcggt
     1681 agaaccagtt tttgcccttg ggcgtcgaag tgtagtccag cgtgccgttg cggtcggcca
     1741 gccggagcat caaaacttcc tgcacgacgt agtcgggatg cggctcaaag gcgacttcgt
     1801 cgaagttgac gtagtcgtaa tcctctccca gaagatactc cccgcgccgt tgcgatgaac
     1861 gggcggagaa aagcgagccg tttttcagtt caagttgcgg aaaaggggtg aaacgcaggt
     1921 cggcaatcaa gtgtccgagt ccgggtcttt gacgaatcag ccgcaggacg tgattgaaga
     1981 cgagattcgc ctgattctgg gtgatcgaca cattcaccgc agtgtagcgt tggtcggtga
     2041 ttttcggatc gcggcggatt tgaaagatca gccgatgcag gaatttcacc gcctgaatga
     2101 gactcttgcc ccagcggttg ccggcgtgga gaacgttttc ggcttgggta gagttggaga
     2161 gccagcgctt ctggccctcg tggaggttga cgccgaggta ggcttcggaa aaatagactg
     2221 gatcaagcca gcattttttc catttttcac ggaaggttgg tgagatgtgc atgagaattg
     2281 agaaagagcc aaaaccgagg cggggacgcc tcggctatgg aggcaaaggc aacccctccc
     2341 taccctcccc tcaaagggga ggggtaatga cctcacccga ccctctcccc ttcgacaggc
     2401 tcagggtggt gagcgaagcc gaaccactac aaggagaggg agtgaagttc acccttcatt
     2461 ctttctattg aaggtgaagt atggattttc cctcccctgt aggggagggc agggtggggt
     2521 cagcgttgct aattactcaa aggctctttg ctttgcttca tcaaacgtca aaaatccggc
     2581 gcgctgctgt ttcaaaacat tatcaaggtg catctgctcg gctctgcgtg cttccaaaag
     2641 gccgaaagtc cgccggttgt cgaagtggtg ctcaactcgc aaatcaagtc catgcagggc
     2701 caactccaaa ttgtgcagcc actccacgaa gcgtttggcc ggacgctgca ccgaaatcac
     2761 ctgccgctcg accaactcca acttaaaccc ggcccagtgc tgagtggtgc cataggagta
     2821 acccaacata aagggatcga gatgcgtgcc ggaggcgatg tcttccacca tcgccttatg
     2881 attcaaatac cagccggacg cgcccgcata ctgtcccgtc gggccgatgt aggcgatttc
     2941 cacctcgttc caggtgaccg ggttatcctc cggcgcgatc tttttcatca tccccacggt
     3001 gtcttcaaag tagcggttcg cccgcgccac gtaggcatcg tccgattcgg cgtccttttt
     3061 ctccggcggt ttgatcttca cgtgcaggcg gtggtagccg gcgttgtgca tcgtcttctg
     3121 catatcggcg actaactgct gttccacccg cgtcacaaat ggaattccgg agagaagact
     3181 tctcccgcgc gggttggcga cgtcggtatc gagaccgaag taaaaggtcg agtggggatt
     3241 cagacgaatc ggccggtctt cggtgacctg atacagttcc agctgtccgt ttctgttcgg
     3301 aatgaactca atcgtcgctg gatcgatgaa gtagaagcgg tcaatccggt cgccggcagg
     3361 ggagaggacc acttcgccgc agaccgcgcc gtcggtaaag agcccggtga agaattgcgc
     3421 caagagcatg tccaacccgc tgaatttggc gaagtcgtgc tcgaaaatgc gctggtctaa
     3481 gtcgaccaga atttcctccg ctctggtttt gagtgccgcg ttgtcggttt tgacttcagc
     3541 gcgattcggg ctggtgcaga ggcgcgtcca ggtccagatg gcggagttta
//
LOCUS       DBNGZD010000297         6261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1854209, whole genome shotgun sequence.
ACCESSION   DBNGZD010000297 DBNGZD010000000
VERSION     DBNGZD010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1854209"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="AC16GM_07910"
     CDS             complement(<1..620)
                     /locus_tag="AC16GM_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR2923636.1"
                     /translation="MTQPQEPQKTIDLAISGMTCASCVSTVEKALKSVSGVTQASVNL
                     ATERALVTYRPGVATLEQMFKAIADSGYKARELSAETRDEREELLKEELTTLKKKLFF
                     SLIAAALLMLLGFQMYLPGLKAFPRSLIPWLGLVLATPVQFWAGARFYRGFWAQFSAG
                     IFSPWPASPTDASRMRASMSAVASSTRQCSMMGSATFSAVVREFRSA"
     gene            complement(676..1374)
                     /locus_tag="AC16GM_07915"
     CDS             complement(676..1374)
                     /locus_tag="AC16GM_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR2923637.1"
                     /translation="MIKIRKADERGHADYGWLSTYHTFSFDTYYDAAYMGFRSLRVIN
                     EDRVQPGNGFPSHFHRDMEIISYVLEGALEHRDSLGTGSIIRPGDVQRMSAGTGVTHS
                     EYNPSQTDIVHFLQIWILPERKGLKPSYEQKRFSDEEKRGTLRLIASREGRDGSVSIH
                     QNASVYATSLDIGQELVHPLKPGRHAWAQVVRGRATFNDRELKQGDGAAASEEKALKL
                     ASREGAEVLVFDLA"
     gene            complement(1422..2465)
                     /locus_tag="AC16GM_07920"
     CDS             complement(1422..2465)
                     /locus_tag="AC16GM_07920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2923638.1"
                     /translation="MADGSKNLNEICDITIIGAGPVGLFAAYYAGLRALRAKVIDSLP
                     KVGGQLTSLYPKKNIYDVAGFPKILAEDLVQNLLVQALQYRPELCLEQRIQKLEYVDG
                     NLIQLSDENGIRHLTRTVIISAGTGSLVPRRLELPGVEKYLDHGLTYVVLDPEEYRGK
                     RVLIVGGGDTALDWALTLKDIAKETTLIHRSGKFAGHEDSVRKLFASHVRTHIHTEIK
                     TIHGDSRVAGATITTREKNEEITLDLDAIIVCIGFTPNLGAIKEWGLAIEKNSIQVNS
                     RMETNLPAVYAVGDVVDYPGKVKLIVTGFGDAAVAVCHAAHRINPQLKVFPGYSTNFV
                     EKLQAKVQTSVGG"
     gene            2649..4586
                     /gene="mnmG"
                     /locus_tag="AC16GM_07925"
     CDS             2649..4586
                     /gene="mnmG"
                     /locus_tag="AC16GM_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR2923639.1"
                     /translation="MIVVGAGHAGCEAALVCARMGLRTSLFTQNRDTIAQMSCNPAIG
                     GQAKGQLVKEIDALGGEMGWVADQTGVQFKILNKSKGPAVWSSRSQNDRLLYKLCMRQ
                     VVTSQAGLEIVEDGAIDILTTGGRATGVVGSSGNRYKAQAVVLCSGTFLNGLIHIGKK
                     NFPAGRVGESPALYLSENLQKLGFETGRLKTGTPPRLSHKSIDYSKTELQPGDDPPVP
                     FSLRTDKIKNPQLFCWLTYTSAETHQIIRDNIHQSPMYSGQIKGIGPRYCPSVEDKIF
                     RFADKPRHQIFFEPEGYETDVVYINGFSTSLPEEVQFAALKTIPGLEQVEMLKPGYAV
                     EYDFFPPTQLKHSLETKLIENLFFAGQINGTSGYEEAAAQGIMAGINAALKIKGEEPF
                     VLDRSEAYIGVLIDDLVTKNPLEPYRMFTSRVEYRLAIREDNAADRLMPYGYKLSLVP
                     KRLYQKYQERRGEIEREKDRLQKSRYRRGELFSLLMNGNRTAEREGEHDDPLVIEAED
                     RPPDIARTNDNGKSPNPDQKISLFTFLRDPRVNYRHIREIDSESVCVRPQIAQQVELE
                     VKYEGYILRQEKEIAKFKKTEGMRIPERINYLSLKGFKREAQEKLQRIRPATVGQASR
                     ISGVTPGDIAVLMVHLKQLES"
     gene            4633..5298
                     /gene="rsmG"
                     /locus_tag="AC16GM_07930"
     CDS             4633..5298
                     /gene="rsmG"
                     /locus_tag="AC16GM_07930"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR2923640.1"
                     /translation="MKHCPSPKVEQTKIDLPSALKLINLEPSNLQLSQFYIYFDLVLK
                     HWKKFDLVAPSQANQFGLARQTVDSLSVLRAIDFPQNAKIADVGSGAGFPGIPLAIVR
                     PDLQLTLIDAKPKKSLFLQECRTVLKLSFQIIQSDVRRVTEQFDFTLYKAFADLKMVV
                     RLSRRLLVSGGVAVAYKTKNYPSELKKLKFFTGTARLESAENVPVPELNLNTCFLIFR
                     KLD"
     gene            5410..6249
                     /locus_tag="AC16GM_07935"
     CDS             5410..6249
                     /locus_tag="AC16GM_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR2923641.1"
                     /translation="MGKVIAVVNQKGGVGKTTTAINLSACLAAAERKTLLIDIDPQAN
                     ATSGIGLDKSKIKTTIYEALLEELPLREIILKSELAYLDVAPSHTRLVGAEVELVNAI
                     GREKRLTTAVSPIRNSYDFIFVDCPPSLGLLTVNTLTAADSVLIPIQCEYYALEGLGQ
                     LLNTIRLIQKHLNPGLAIEGVLLTMYDSRLNLSKQVADEARKFFTDKVYQTIISRNVR
                     LSESPSFGKPIILYDILSTGAENYIALAKEFLERNGADPQALEPAGVTRTILSELEPA
                     AVK"
BASE COUNT         1509 a         1682 c         1614 g         1456 t
ORIGIN      
        1 gcgctcctga actccctcac caccgccgag aacgtggcgc tgcccatcat ggagcactgg
       61 cgcgtcgacg aggcgaccgc gctcatgctg gcgcgcatgc ggctggcgtc ggtggggctc
      121 gcgggccacg gcgagaagat gccggcggag aactgcgccc agaaaccccg atagaagcgc
      181 gccccggccc agaactggac cggtgtcgcc agaaccagtc cgagccaagg aatgagggaa
      241 cgcgggaatg ctttcagccc cggcaggtac atctggaaac ccaagagcat caggagagcg
      301 gcagctatca gactgaagaa tagtttcttc ttcagagttg ttaactcttc tttgagtaat
      361 tcttcccgct cgtcgcgcgt ctctgcggat aactccctgg ccttgtatcc ggaatcagcg
      421 attgctttga acatttgttc gagcgtggcg actcccggtc tgtaggtgac cagtgctcgc
      481 tcggtggcta agttgacgga ggcctgcgtt actcctgaca cagacttcag cgctttttca
      541 acggtggaaa cgcacgaggc acaggtcatg ccggagatgg ccaagtcgat ggttttttgc
      601 ggttcttgcg gttgggtcat gggcgtttca aaagagaata cgtggagctg ttggtattac
      661 cgactcataa ctttgctacg ccagatcaaa aacaagaact tcggctcctt cacggcttgc
      721 aagcttcaat gctttttcct cactcgcagc tgctccgtcg ccctgtttta actcgcgatc
      781 gttgaatgtt gcccggccac gcaccacctg tgcccaggca tgccggcccg gctttagggg
      841 atggacaagt tcttgtccga tatcaagtga agtggcgtag acgctggcat tttgatgaat
      901 gctaaccgat ccatcgcggc cctcgcggga ggcgatcagg cgcagagtgc cccgcttttc
      961 ttcgtctgaa aaccgcttct gctcatagct cggctttaac ccttttcgtt ccggcaaaat
     1021 ccagatttgc aagaagtgaa caatgtcggt ttgcgaagga ttgtattcgc tgtgcgtcac
     1081 gccggtcccg gcgctcatcc gctggacatc acccggtcgg ataatggaac cagtacccaa
     1141 actatccctg tgctccaggg caccttccag cacgtaggaa atgatttcca tatcccggtg
     1201 aaagtgcgac ggaaatccat tccctggttg cacgcggtct tcgtttatga cccgcagcga
     1261 gcgaaacccc atatacgcgg cgtcgtagta agtgtcgaac gagaaagtgt gataggtact
     1321 cagccaaccg tagtccgcgt gtccacgttc gtcggccttc cgtatcttaa tcaacctttt
     1381 ctcctgcaga ttagattgct tgacttcgat tccaaactgg cttaaccccc tacagaggtc
     1441 tgcactttgg cctgcaattt ctccacaaaa ttcgtcgagt acccggggaa gactttcaac
     1501 tgcggattga ttcggtgcgc cgcgtggcaa actgccacag ccgcatcgcc gaagccggtg
     1561 actattaact taacttttcc ggggtagtct accacgtcgc ccaccgcata gacggccggc
     1621 aggttggtct ccatccggct gtttacctga atcgagtttt tctcgatagc cagaccccac
     1681 tctttaatcg cccccaggtt gggcgtgaag ccgatgcaca cgatgatggc gtccagatcc
     1741 agggttatct cttcgttttt ctcgcgggta gtaatggtag cgccggcaac ccgactatcg
     1801 ccgtgaatcg tttttatttc agtatggatg tgggtccgga cgtgggaagc gaagagtttg
     1861 cggaccgaat cttcatgtcc cgcaaatttt ccggaacggt gaatgagggt cgtttctttg
     1921 gcaatatctt tcagcgtcag cgcccagtcc agcgccgtat cccccccgcc cacaatcaga
     1981 acccttttgc cgcgatactc ctcgggatcg agcaccacat aggtgaggcc gtgatcgaga
     2041 tacttttcga cgcccggcaa ctccagccgc cgcggcacga gcgacccggt gcccgccgag
     2101 atgatgaccg tacgggtcag gtgtcggatg ccattttcat ccgaaagttg aatcagattg
     2161 ccatcgacgt attctaattt ctgaatgcgc tgctccaggc agagttccgg gcggtactgc
     2221 agggcctgaa cgagaagatt ctgtaccaga tcctcggcta agattttggg gaaaccggcc
     2281 acgtcgtaaa tgttcttctt cgggtagaga ctggtgagct gcccgcccac tttcgggagg
     2341 ctgtcgatca ccttcgcccg cagcgcccgc agcccggcgt agtacgcggc gaaaagcccc
     2401 accggccctg caccgatgat ggtgatgtcg caaatctcat ttagattttt agaaccgtca
     2461 gccattcgca aagtgctcct ttaagggaaa gctgctccac ttctcaaatt acgtacagaa
     2521 accgaaaaag tctatggaga atcgtcccgg accggcattt gacagccatc gctgaagtat
     2581 tattttgaga aaaaatcaaa aatgaaaaag ggaaaatgtc aaatttaacc tataaagaaa
     2641 attatgatgt gattgtagtc ggtgccggcc atgccggctg cgaagcggct ttggtttgcg
     2701 cgcggatggg tctgagaacc tcccttttta cccagaaccg agataccata gctcaaatgt
     2761 cgtgcaatcc ggccatcggt ggccaggcca agggacagtt ggttaaggaa atcgacgctt
     2821 tgggcggtga gatgggctgg gtggccgatc agaccggggt gcagttcaaa atcttgaaca
     2881 aaagcaaggg ccccgcggtt tggtcctcgc gctcccagaa cgatcgcttg ctctacaaac
     2941 tttgcatgcg ccaggtggtg acgtcccagg cggggcttga gatcgtcgaa gatggcgcca
     3001 ttgacattct caccacgggc ggcagggcca ccggcgtcgt tgggtcgtcc ggcaacagat
     3061 ataaagcaca agccgtggtc ctctgctccg ggacattttt gaatggctta atccacatcg
     3121 gaaagaagaa cttcccggcc ggacgggtgg gagaatcgcc ggccctctac ttgagcgaaa
     3181 atttgcagaa actggggttt gaaaccggcc ggctcaaaac tggcactccg ccgcgcctct
     3241 cgcacaagag tatcgactat tcgaaaacgg agctgcaacc gggtgacgat ccgcccgtgc
     3301 ctttttcgct gcgcaccgac aaaatcaaaa atccgcaact tttttgctgg ttgacctaca
     3361 cttcggctga gacgcatcag attattcgtg acaatatcca tcaatcgccg atgtattcag
     3421 gacaaattaa gggcatcggc ccgcgctatt gtccttctgt ggaggacaaa atcttccgct
     3481 tcgccgacaa accacggcac cagatcttct tcgaaccgga ggggtatgag acggacgtag
     3541 tctacatcaa cggcttttct accagtctgc cggaagaggt acaatttgca gccctcaaaa
     3601 ccatccccgg gctggaacag gttgaaatgc tcaaaccggg ttatgcagtg gaatacgatt
     3661 tttttccgcc gacgcaactg aagcactcgc tggaaaccaa acttattgag aatctttttt
     3721 ttgccggtca gattaacggc acttccggct acgaggaagc cgccgcccag gggattatgg
     3781 ccggcatcaa tgccgcttta aagatcaaag gcgaagaacc attcgtgctc gaccgctcag
     3841 aggcctacat cggcgttttg attgacgact tggtgacgaa aaatccgctg gagccgtacc
     3901 ggatgtttac ttcccgcgtc gaataccgac tcgctatccg ggaagacaac gccgcggacc
     3961 ggctgatgcc ctacggatac aaattgagtt tagttccgaa gcggctctat cagaaatacc
     4021 aagagcgccg tggagaaatc gagcgggaga aggatcgact gcaaaaaagc cggtatcggc
     4081 ggggagagct gttctcgctt ttaatgaatg gaaatagaac ggcggagaga gaaggcgagc
     4141 acgacgaccc gctggtcatt gaggccgaag accggccacc ggacattgcg cggacaaacg
     4201 ataacggaaa gtctccgaat cccgaccaga aaatttctct tttcaccttc ctgcgcgatc
     4261 cccgggtaaa ctaccggcat attcgagaaa ttgactcgga atcagtctgc gttcgtccac
     4321 aaatcgctca gcaagttgaa ttagaagtca aatatgaagg ttacatcctc aggcaagaaa
     4381 aggaaatcgc caagttcaaa aaaacagaag gaatgcgcat accggagagg attaattact
     4441 tatctttgaa aggtttcaag cgtgaagccc aggaaaagtt gcagcgcatc cgcccggcta
     4501 cggtggggca agcctcacga atttcgggag ttacgccggg agatattgcc gttctgatgg
     4561 tccatctgaa acaactcgag agttgacacg cggcaagaat atgtaaatca ccgagttatt
     4621 aaactgtttc acgtgaaaca ttgtccttca cccaaagtag aacaaacaaa aatcgatctc
     4681 ccttctgccc ttaaactaat aaatttagaa ccctcgaatc ttcaattaag ccaattttac
     4741 atctattttg atctggtcct caaacactgg aaaaaattcg acctcgtcgc cccctcccag
     4801 gctaatcaat tcggcctcgc ccggcagacc gtcgattccc tctcggttct tcgggcaatc
     4861 gattttcccc aaaatgcgaa gatagccgat gtcggcagcg gcgccggttt tccgggaatt
     4921 cccctggcca tcgttcgacc tgacttgcaa ctcacgctca tcgatgccaa gcccaaaaaa
     4981 tccctctttc ttcaggagtg ccggacggtc ctaaaattgt cttttcaaat tatccaatct
     5041 gatgtccgca gggtcacgga acaattcgac tttactctct acaaggcttt cgccgatttg
     5101 aaaatggtgg tgcggctatc ccggcggctc ttagtctcag gcggagttgc cgtggcctat
     5161 aaaacaaaga attacccgtc cgagttgaaa aaactaaaat tcttcaccgg cactgcgcgg
     5221 ctggaaagcg ctgagaacgt tccggtccct gaattaaatc tcaatacttg ctttctcatt
     5281 tttcggaagt tagattaaga aagaaggaat agcatttctc tgtgccctcc gtgtctccgt
     5341 ggtgaaagaa aaaagttctt gcttttatct accctaattg cttaagtttg tcctaaatgg
     5401 ggatgtttat tgggtaaagt aatagccgtc gtcaatcaaa aaggcggagt tggaaagact
     5461 accaccgcaa ttaatctgtc ggcttgcttg gccgccgccg agcgtaagac tcttttgatc
     5521 gatatcgacc ctcaggctaa tgctacttcc ggcatagggt tagacaaaag taaaatcaaa
     5581 acaacgattt atgaagctct tttagaggaa cttccgttac gcgagataat tctcaaatcc
     5641 gaactggctt atctggatgt ggctccctca catacccgtt tggtcggggc cgaagtagaa
     5701 ctggtcaatg caatcggcag ggaaaagcgg ctgacaacgg ccgtgtcccc cattcggaat
     5761 tcatatgatt tcattttcgt cgattgtcct ccctcgctgg gcttgctcac cgttaacacg
     5821 ctgacggcag ctgattcggt tttaattccg attcaatgcg aatactacgc gctggaaggt
     5881 ctgggccagc tcttgaacac cattcgacta attcaaaagc acctgaaccc gggtctggca
     5941 attgagggtg tcctcttgac gatgtatgac agccggttga atctctccaa gcaggttgca
     6001 gatgaagcgc gcaagttctt taccgacaag gtgtaccaga cgatcatcag ccgcaacgtc
     6061 cggctttccg aatcgcccag tttcggcaaa ccgatcattc tgtacgacat tttgtccacc
     6121 ggcgcggaaa actacattgc tctggcgaaa gaattcctgg agcgaaacgg cgcggaccct
     6181 caagcattgg aacctgcggg cgtgacgagg acaattcttt cggaattgga accggcggct
     6241 gtgaaataaa ctacggaggc g
//
LOCUS       DBNGZD010000298         8975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1159525, whole genome shotgun sequence.
ACCESSION   DBNGZD010000298 DBNGZD010000000
VERSION     DBNGZD010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1159525"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /gene="glpX"
                     /locus_tag="AC16GM_07940"
     CDS             complement(<1..556)
                     /gene="glpX"
                     /locus_tag="AC16GM_07940"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HIR2923642.1"
                     /translation="MDRNLALELVRVTEAAALSAARWMGKGDAMAADQAAVEAMRKAF
                     ASVEFDGTIVIGEGERDEAPMLFIGEKVGTGRSAQVDIALDPLECTNSVAYGRLNAIS
                     VIAAAERGNFLHAPDTYMEKIAVGPGAEGAIDLDLSPAENLRRIAEAKGYKVDDLTVV
                     ILDRPRHAELIKSVRQAGARIQLIP"
     gene            complement(626..2104)
                     /gene="rpoN"
                     /locus_tag="AC16GM_07945"
     CDS             complement(626..2104)
                     /gene="rpoN"
                     /locus_tag="AC16GM_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR2923643.1"
                     /translation="MKLGLQAKLRQTIAPQLIQSLHLLQVPILKLEQLLRVELSTNPL
                     LEEAEALEEEPETDTATPETESADGPNDPELNKIDWDNYLDEDAKATDYAIAAHREKD
                     QDEPLQTPVSEKTLYEHLADQLSLHRVTEEERQIGLFIIGNIDQSGFLTISVEEIAET
                     LQVPVAAVEKVLKVIQGFDPVGVGARDLREALLIQMRERGLEDTLPWQIVAEQLTDLD
                     RKSNSQLARLLGVELERVEEAMAVIRTLSPRPATGRFVTAAQPVVPDLTVERIGEEFV
                     VYHNDRNIPRLRINPAYREMLRRNSSSAAETKSYVREKLEQARWLLNAINQRRNTMIK
                     VMNAVVETQKNFFEHGPEHLKPLTMEEVADRIGMNVATVSRVANDKYVQTPFGVFEIR
                     RFFNQGVPQVGGEDISKQTVKERITQLIREENPAQPLSDQEIFEKLKAERLTLARRTV
                     TKYREELNIQAARFRKRSVKANGDTPLPVAPGPTPNSFGQSS"
     gene            complement(2264..3001)
                     /gene="lptB"
                     /locus_tag="AC16GM_07950"
     CDS             complement(2264..3001)
                     /gene="lptB"
                     /locus_tag="AC16GM_07950"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR2923644.1"
                     /translation="MNNHNQTLRSENLVKIYRNRRVVDGVSIDVKQGEVVGLLGPNGA
                     GKTTTFHMVIGFIQPNGGRVLIDDTDITDLPMYRRARRGIGYLAQEPSVFRKLTVEKN
                     ILAILEMQKLTHQERKERLKTLLAELDLTGLARRKAYTLSGGERRRVEITRALVNEPK
                     FLLLDEPFLGIDPIAVEEIQKIINRLKTRGLGILITDHNVRETLSITDRAYIMSDGEI
                     LKSGTAEFLASDPEARKIYLGEKFRLN"
     gene            complement(3039..3419)
                     /locus_tag="AC16GM_07955"
     CDS             complement(3039..3419)
                     /locus_tag="AC16GM_07955"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR2923645.1"
                     /translation="MFTLVEAKVEFDGKRNVFKFFLQPIEKKAFRKERVVEHTRLVPT
                     HVKIAVWKRDYGSCVLCGAKENLHFDHEIPFSKGGSSITVQNVRLLCAQCNISKGSKI
                     MVFIPFLVSSLSVSRIDGLYSQVN"
     gene            complement(3407..3781)
                     /locus_tag="AC16GM_07960"
     CDS             complement(3407..3781)
                     /locus_tag="AC16GM_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923646.1"
                     /translation="MRDDILSYEEIVQKEGMLLQKGMNFRVGKGYSILLMSVRKGAPY
                     RDEFDQDTGLLIYEGHDVATKKTDPKSIDQPMSFPSGKLTENGNFTKRQKISKREQHL
                     VSVCKCMRKSYGEYGVTKECLR"
     gene            complement(3787..4341)
                     /gene="lptC"
                     /locus_tag="AC16GM_07965"
     CDS             complement(3787..4341)
                     /gene="lptC"
                     /locus_tag="AC16GM_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR2923647.1"
                     /translation="MKRFVVVPIVLFVLLLACSRQEEPKPSAVLTGVPDQVLENSVVT
                     LTDKGVLSTYLLAKKVARYEAPDITKVWEIKADFFDHEGKHFSVLVADSGLVREKTRR
                     LTAWGHVVVTYDRGDRFESDSLFWDGEKEHVETDAFVKIFRKDGNMQGYGLETDSEFK
                     RFKIKRQVKGTYTEMPADSVLEKK"
     gene            complement(4397..5323)
                     /locus_tag="AC16GM_07970"
     CDS             complement(4397..5323)
                     /locus_tag="AC16GM_07970"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR2923648.1"
                     /translation="MEAEAVAGLGRKLNGQFVRAIELLLTCKGRVIVTGMGKSGIISR
                     KIAGMLNSTGTPAIFLHPAEGAHGDLGFVQRGDVVIAISKSGGTDEVYQLFPVFKRLG
                     VPVITITGNPKSPMAEKSDVVLDVSVAEEACPNDLVPTSSSTAALVMGDALAVVLLAE
                     RHFSPEEFALLHPGGSLGKKLLLKVADLMHTGDQVPKVSLNTPVKEAILEMTSKRFGA
                     TSVVNGKGELVGVFTDGDLRRLVEKTGDIYTVTMEKAMTKSPKTIDPEALAAAALNKM
                     ELHKITQLLVVDGARQPIGIVHLHDLLQAGVV"
     gene            complement(5468..5983)
                     /locus_tag="AC16GM_07975"
     CDS             complement(5468..5983)
                     /locus_tag="AC16GM_07975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR2923649.1"
                     /translation="MKRKGNLKRRLLPIKLVILDADGVLTDNCIFISDSGEKWQKFSI
                     ADGMGISLGQKHGLTFAVISGNPSQAIAHRARELGVPDCFVGVKSKVPIFEQLKAKYG
                     AANAEIAYVGNDINDLALLRQVGLAACVRDAIGDIQKHCHYVAKHPGGECAVREIVEL
                     ILKGQKKKLVW"
     gene            complement(5980..6798)
                     /gene="kdsA"
                     /locus_tag="AC16GM_07980"
     CDS             complement(5980..6798)
                     /gene="kdsA"
                     /locus_tag="AC16GM_07980"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR2923650.1"
                     /translation="MSSTVKIGNFIVGRGNLLLIAGPCVIESETMVLRTAKRLKELAE
                     RCGFFLVFKSSYQKANRLSGKSYAGPGLDAGLKVLEKVKREFELPLTADIHSAGEAAS
                     VGEIVDLIQIPAFLCRQTDLVAAAARTGKAVNIKKGQFVAPEDMKSIAEKAIRAGNRN
                     ILLTERGTAFGYHNLVVDFRSLVIMRNLGYPVVYDATHSLQLPGANGATSGGQREFAL
                     PLARAAIACGVDGIFLETHPDPSRALSDRETQIPLAEMPKFLDALKRITEVSNR"
     gene            complement(6914..7303)
                     /locus_tag="AC16GM_07985"
     CDS             complement(6914..7303)
                     /locus_tag="AC16GM_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923651.1"
                     /translation="MAAFAVAFLIPQCGGTPDSGGPIKSRFPIPPIEEGLQIQAQLDE
                     GHQPWRLDPKQVVIAYAAEIGIKNEVMSGDVLLAKDDSMLSIVEVNSPKSQFRRRVYL
                     ARLLENPQNRFTIWFVVGLQNIPKKKA"
     gene            complement(7363..8973)
                     /locus_tag="AC16GM_07990"
     CDS             complement(7363..8973)
                     /locus_tag="AC16GM_07990"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR2923652.1"
                     /translation="MSAASIGALMECRGLTVTLLKMDPYINVDPGTMNPFQHGEVFVL
                     DDGAETDLDLGHYERFLDENLTRANNVTTGQVYEAVITRERKGDYLGATVQVIPHVTN
                     EIKERIKKVSHNEHDYDVVITEIGGTVGDIESQPFLEAIRQMGLEEPGENVLYIHLTL
                     VPFIDTAGEVKTKPTQHSVKALREIGIQPDILVCRTARPLSTELKEKIGLFCNVPSGA
                     VIQAIDVDSIYEVPLLFHREGFDDLAVRHLQLNYPEPDLAPWEKIVHKIKNPAYRIKI
                     GICGKYVNLKDAYKSIIESFAHAGIENDAHVELEWISSENIKREGAEKYLNNLDGLLI
                     PGGFGDRGVEGKIEAIRYARESRLPFFGICLGMQCATIEYARNVCGLAQAHSSEFNPQ
                     SSQRVIDLLPEQHQVTQMGGTMRLGAYPCKLDRHSLAFKAYGSEEISERHRHRYEFNN
                     EYRKILAEAGFRITGTSPDGRLVEIIELSDHPWFVGVQFHPELKSRPTKAHPLYREFV
                     RAALKHHLAQKESTPEPVPEAQVKAAVT"
BASE COUNT         1755 a         2639 c         2448 g         2133 t
ORIGIN      
        1 ccggaatcaa ctgaattcgc gccccggcct ggcgcacgct cttgatcaac tcggcgtgcc
       61 gcggtcgatc cagaatcacc accgtcaggt catcgacttt gtaccccttg gcctcggcaa
      121 tccggcggag gttttccgcc ggcgaaagat cgagatcaat ggccccctcc gctcccggcc
      181 cgaccgcaat tttctccatg taggtgtccg gcgcgtggag gaagttcccc cgttccgcgg
      241 cggcgatcac cgaaatggcg ttcagccgtc cgtaggcgac gctgttggtg cactccaaag
      301 gatcgagcgc aatatcgacc tgggccgagc ggccggtgcc caccttttcg ccgatgaaaa
      361 gcattggcgc ctcatcccgc tctccttcgc cgatgacgat agtgccgtcg aattcgaccg
      421 aagcgaaggc tttgcgcatg gcttcgaccg cggcctggtc ggcagccatg gcatcccctt
      481 tgcccatcca gcgggcagcc gaaagcgccg ccgcttcggt aacgcgcacc agttccagcg
      541 ccaggtttcg atccatttca gttggaattt agccaaaaga caaaaaaaac caagccgggg
      601 gcttggcggt gcgagttagg agcaattatg acgactgccc gaatgaattc ggggtagggc
      661 cgggggcaac ggggagcgga gtatcgccgt tggctttcac cgaccgtttg cggaaacggg
      721 ccgcctggat gttcaattct tcgcggtact tggtcaccgt gcggcgggcg agcgtcaacc
      781 tctcggcttt cagtttctca aatatctcct gatcggagag cggctgcgcg ggattctcct
      841 cccggatcag ttgggtaatc cgctccttga ccgtttgctt ggaaatgtct tcgccgccca
      901 cctgcggcac cccctggttg aagaagcggc ggatttcaaa gactccaaag ggcgtctgta
      961 cgtacttgtc gttggccacc cgggagacgg tggccacgtt catcccgatc cggtcggcca
     1021 cctcttccat ggtcaggggt ttcaagtgct ccggcccgtg ttcaaagaag tttttttggg
     1081 tttccaccac ggcgttcatc accttgatca tcgtgttgcg cctttggttg atggcgttca
     1141 aaagccagcg cgcctgctcc aatttctccc gcacgtagct tttggtctcg gcggcggaag
     1201 acgagttgcg ccggagcatc tcccgatagg cggggttgat gcgcaggcgc gggatgttcc
     1261 ggtcgttgtg gtagacgacg aactcctcgc caatccgctc cacggttaaa tccggcacca
     1321 ccggctgggc cgcggtgacg aagcggccgg tcgcgggtcg gggggagagg gtgcggatca
     1381 ccgccatcgc ttcttccacc cgttccagtt ccacgcccaa aagccgcgcc agctgggagt
     1441 tgctcttccg gtcgagatcg gtgagctgct cggcgacgat ttgccagggc agggtatctt
     1501 ccagaccccg ttcgcgcatt tgaatcaaaa gcgcctcccg gaggtcgcgg gcccccaccc
     1561 ccaccggatc gaatccttga atgaccttca aaactttctc caccgcggcc accggcacct
     1621 ggagcgtctc ggctatttcc tccaccgaaa tcgtcaggaa accgctctgg tcgatgttgc
     1681 caataataaa gaggccgatc tgccgctcct cttcggtgac ccggtggagg gagagctggt
     1741 cggctaagtg ttcgtagagg gttttttcgg aaacgggagt ctggaggggt tcgtcctgat
     1801 ctttttcccg atgggcggcg atggcgtagt cggtggcctt ggcgtcttcg tctaagtaat
     1861 tgtcccaatc gatcttgttc agctccgggt cattgggacc gtcggctgat tccgtttcgg
     1921 gggtggccgt gtcggtttcc ggttcttcct ccaaggcctc ggcttcctcc aggagcggat
     1981 tggttgacag ctcgacccgc agcagttgct ccaatttcaa gatcggcacc tgcagcaaat
     2041 gcagagactg aatcaactgg ggggcgatgg tttgcctgag ttttgcctgg agtcctaact
     2101 tcataattga ctcttcaccg cctggtctgc gtccctactg cgatcccgcc gtgaccgggg
     2161 cttgacctca gaatagccgc ccccggcctg cgcaaaaatt gcacagccct cgaccctggc
     2221 gttggttccg ggtactgatt cagttgtcgg cggaaagaga gccctaattt aaccgaaact
     2281 tttcgccaag gtagatcttg cgcgcctccg ggtcgctggc caggaactcg gccgtgcctg
     2341 acttcaaaat ctcaccatca ctcataatat acgcacggtc ggtaatagac aaagtctccc
     2401 ggacgttgtg gtcggtgatg aggatgccca accccctggt tttcagccgg ttaataattt
     2461 tttgaatttc ttcgacggcg atcgggtcga tgcccagaaa cggctcatcc aaaagcagga
     2521 acttgggctc gttgaccaga gcccgggtga tctccacccg ccgcctctcc ccgccggaga
     2581 gggtgtaggc cttgcgccgg gctaagccgg tgaggtcaag ttcggccagg agggttttga
     2641 gcctttcctt ccgctcctgg tgggtcagtt tctgcatttc cagaattgcc aggatgttct
     2701 tttcgacggt cagtttgcgg aaaaccgacg gctcctgcgc caggtagccg atcccccgcc
     2761 gggctcttcg atacatcggc aggtcggtga tgtcggtgtc gtcgatcaac accctgccgc
     2821 cattgggttg gatgaaaccg atgaccatat gaaaggtggt agtcttgccg gcgccgttcg
     2881 ggcccaaaag ccccaccacc tccccctgct tcacatcaat agacacccca tccaccaccc
     2941 gacggttgcg gtagattttg accaaattct cggagcggag ggtttggtta tggttgttca
     3001 tagttagtgt tagctatttc cagttacccc tgtgtacttt agtttacttg agaatatagg
     3061 ccatcaattc ggcttaccga gagtgaactc accaagaatg gaataaatac catgatcttg
     3121 cttcccttgc taatattaca ttgggcacaa agtaagcgca cgttctgtac agtaatacta
     3181 cttccgcctt ttgaaaaagg tatttcatga tcgaagtgta aattttcttt tgctccacac
     3241 aacacgcacg acccataatc tcttttccaa actgctatct taacatgagt tggtacaagg
     3301 cgagtatgtt ctactactct ttctttcctg aaggccttct tctcaatcgg ttgaagaaaa
     3361 aatttgaaaa cattccgttt tccatcaaat tctactttcg cttcaactaa cgtaaacatt
     3421 cctttgtcac tccatactcc ccgtaagatt ttctcataca cttgcacacg ctcaccaggt
     3481 gctgctcccg ttttgaaatc ttctgccgct ttgtaaaatt tccattttcg gtaagcttcc
     3541 cactcgggaa actcataggt tgatctatag acttcgggtc ggttttcttt gtggcaacgt
     3601 catgtccttc ataaataagc aaaccggtat cctggtcaaa ttcgtctcga tatggagctc
     3661 ccttccgaac tgacattaaa agaattgaat atcctttacc gactctaaaa ttcatccctt
     3721 tctggagaag cattccttcc ttttgaacaa tctcctcgta tgacagaata tcgtcacgca
     3781 tattacctac tttttctcta ataccgaatc cgccggcatt tcggtgtagg tgcccttcac
     3841 ctggcgtttg attttgaagc gtttgaactc ggagtcggtc tccaggccgt agccctgcat
     3901 attgccgtct ttgcggaaaa tcttcacaaa ggcgtcggtt tccacgtgct ctttttcgcc
     3961 gtcccagaag agactgtccg actcgaaccg gtcgccccgg tcgtaggtca ctaccacgtg
     4021 gccccaggcg gtgaggcggc gcgtcttttc ccgcaccagg ccggagtcgg ccaccagaac
     4081 cgaaaagtgc ttgccctcgt ggtcaaagaa gtcggccttg atttcccaga ctttggtgat
     4141 gtccggggct tcgtagcggg ccactttttt ggccagaagg taggtagaga gcaccccttt
     4201 gtcggtcaag gtgacgaccg agttctccaa aacctgatcc ggtacgccgg tcagtacagc
     4261 agagggtttg ggctcctcct gccgggaaca ggcgagaaga agtacgaaga gtacgatcgg
     4321 gaccactacg aaacgtttca tttcgtccgc acttccgtca gaaagattct tttcgacttc
     4381 attcccttaa gatacgttac actacccccg cctgcaggag gtcgtgcagg tggacgatgc
     4441 cgatcggctg gcgcgcccca tcaaccacga gaagctgggt gattttgtgc agttccatct
     4501 tattcaaggc cgccgccgcc aatgcctccg ggtcaatcgt cttggggctt ttggtcatcg
     4561 ccttttccat agtgaccgta tagatgtcgc cggtcttctc cactaagcgc cggaggtcgc
     4621 cgtcggtgaa gacgccgacc aattctcctt tcccgttaac tacggaggta gccccgaagc
     4681 gcttggaggt catttccaga attgcctctt ttaccggggt gttcaaagac accttcggca
     4741 cctggtcgcc ggtatgcatc aggtcggcca ctttcaagag caactttttt cccagactcc
     4801 cgccggggtg cagaagagca aactcttcgg gcgaaaaatg gcgctccgcc aggagcacca
     4861 ccgccagggc atcgcccatc accaaagccg cagttgagga agaggtcggc accaggtcgt
     4921 tggggcaggc ttcctccgct accgaaacgt ccagcaccac gtcgcttttc tccgccatgg
     4981 gcgacttagg gttgccggtt atggtaatga ccggcacgcc cagacgtttg aagaccggga
     5041 acagttggta gacttcgtcg gttccgcccg atttggagat ggcaatcacc acgtcgcccc
     5101 gctggacgaa gcctaaatcg ccgtgggccc cttcggccgg gtggaggaag atggccggag
     5161 tgccggtcga attgagcatg ccggcgatct tgcggctgat gatacccgac ttccccatgc
     5221 cggtgacaat tacccgcccc ttgcaggtca gcaaaagctc aatcgccctg acaaactggc
     5281 cattgagttt ccggcccagc ccggcgaccg cttccgcttc catagcgatc acctgccggg
     5341 catgccgcag aatttcggac tcgccattac gctttttgcg ccccgccacg ggcggtgctg
     5401 gccgttctac cctttcggca acatccgctg ttcgcatatg tccctctttt cccttcaccc
     5461 tgattcttta ccagaccagc ttcttcttct gtcctttgag gatcaattcc acaatttcgc
     5521 ggacggcaca ttcgccgccg ggatgtttag caacatagtg gcagtgcttc tggatgtcac
     5581 cgatagcatc ccgcacgcag gcggctaacc ctacctgccg gaggagcgct aagtcattga
     5641 tgtcgttccc cacataggca atttcggcat ttgccgctcc atattttgcc tttaactgct
     5701 caaaaatagg caccttgctc ttcaccccga caaaacagtc cggcacccct aattcccgcg
     5761 cccggtgcgc tatcgcctga gatggattgc ccgaaatgac ggcaaaggtc aggccgtgct
     5821 tctgccccag cgagattccc atcccgtcgg cgatggagaa cttctgccac ttctcacccg
     5881 agtcggagat gaagatgcag ttgtcggtca agacgccgtc ggcatccaga attactaatt
     5941 tgatcggcag gagcctccgc ttcagatttc ctttgcgctt caccgattgg atacctcagt
     6001 aattcgtttg agcgcgtcta agaatttcgg catttcggcc aaaggaattt gcgtctcccg
     6061 gtcgctcaag gcccgggagg ggtccgggtg cgtttccagg aagataccat ccacgccgca
     6121 ggcaatggcc gccctcgcca agggcagggc aaactccctc tggccgccgg aggtcgcgcc
     6181 attggccccg ggcaattgga gcgaatgggt ggcgtcgtac accaccgggt atcccaagtt
     6241 gcgcatgatt accagcgacc ggaaatcgac caccagattg tggtagccaa aagcggttcc
     6301 ccgctcggtc aagagaatgt ttcgattccc ggccctgatg gctttttcgg cgatagattt
     6361 catatcttcc ggcgccacga actgcccttt tttgatgttg accgctttcc ccgtgcgggc
     6421 ggcggcggcg acgagatcgg tctgccggca gaggaaggcg gggatttgaa tgagatcaac
     6481 aatttccccg acacttgcag cttcaccagc cgaatgaata tcggcggtca aaggtaactc
     6541 gaattcacgc ttgaccttct ccagaacttt caaaccagca tccagtcccg ggccggcgta
     6601 cgatttcccg ctcaggcggt tggctttttg gtaggaggat ttgaacacca gaaagaaacc
     6661 acaccgctcc gccagctctt tcagacgctt ggccgtccgc agtaccatgg tttcggattc
     6721 aatcacgcac ggtccggcga tgaggagtaa atttcctcgg ccaacgataa aatttccaat
     6781 ttttacggtg cttgacatcg gttgtctcac ggataaaaga atatacgctg tccgttccgg
     6841 ccttcaaatc tttttgaccg gctctgggac gagccggtct accggctttt gaattgactt
     6901 caagcgctaa gacttacgct tttttcttgg ggatgttctg caatccgacc acgaaccaga
     6961 tggtgaagcg gttctgcgga ttttccaaaa gccgcgccag gtaaacccgg cggcggaact
     7021 gcgacttggg agaattaacc tccacaatcg acaacatgct atcgtctttg gccagaagga
     7081 cgtcgcccga catcacttcg tttttgatcc cgatctccgc cgcgtaggca atgacgacct
     7141 gcttggggtc gagccgccac ggctggtgcc cctcgtcaag ttgggcttga atttgcaagc
     7201 cctcttcgat gggggggatg gggaagcggg atttgatcgg ccctcccgaa tcgggggttc
     7261 ccccgcactg gggaatcaaa aaagcaacgg cgaatgccgc cactatcagc caagctgatt
     7321 tccttccctg atttgggaag gcgacagacg ttctgtttgc tgtcacgtta cggccgcttt
     7381 cacctgtgct tccggcaccg gttcgggcgt actctctttt tgcgccagat ggtgcttcag
     7441 cgcggcccgg acgaactccc ggtagagggg gtgcgctttg gtcggccggg acttcaactc
     7501 cggatgaaac tgcactccta cgaaccaggg atggtcggac agttcgatga tttccaccag
     7561 ccgcccgtcc ggagaggtgc cggtgatgcg gaagccggcc tctgccagaa tcttgcggta
     7621 ctcattgttg aattcgtagc ggtggcgatg cctttcggaa atttcttcgc tgccgtaggc
     7681 cttgaaggcc agactgtggc gatccagttt gcaggggtag gcacccagac gcatggtgcc
     7741 gcccatttgc gtcacctggt gctgctccgg caacaggtcg ataactcgct gcgaactttg
     7801 gggattgaac tccgaactgt gggcctgagc cagcccgcag acgttccggg cgtattcgat
     7861 ggtggcgcac tgcatcccca gacagatgcc gaagaagggc aggcggcttt cccgcgcgta
     7921 gcggatcgct tcgattttcc cttccactcc gcgatcgccg aaaccgcccg ggatcagaag
     7981 cccgtccagg ttgttcagat acttttcggc gccctcgcgt ttaatgttct ccgaggaaat
     8041 ccattccagt tccacatggg cgtcgttttc tatcccggca tgggcaaacg actcgatgat
     8101 gctcttgtag gcgtccttga gattcacgta cttgccgcaa atcccgattt tgatgcggta
     8161 agcggggttt ttaatcttgt ggacgatttt ttcccaggga gccagatccg gctccggata
     8221 gttaagctgc aggtgccgga ccgccaggtc atcgaacccc tcccggtgga acaagagcgg
     8281 cacctcgtag atcgaatcga cgtcaatcgc ctggatgacc gcgccggacg ggacgttgca
     8341 gaacaagccg atcttctctt tcagttcggt cgaaaggggc cgggcggtgc ggcagaccag
     8401 aatgtcgggc tgaatcccaa tttcgcgcag ggctttgacc gaatgctggg tcggcttggt
     8461 cttcacctcg ccggcggtgt cgatgaaggg caccagcgtc aggtgaatgt agaggacgtt
     8521 ctctccgggt tcctctaagc ccatctggcg gatcgcttcc aggaagggct gcgactcgat
     8581 gtcgcccacc gtcccgccga tttcggtgat caccacgtcg taatcgtgct cgttgtggct
     8641 caccttcttg atccgctctt tgatttcgtt ggtaacgtgg ggaatcacct gcacggtggc
     8701 gcccaggtaa tcgcctttgc gctcccgggt aatcaccgcc tcgtagacct ggccggtggt
     8761 gacgttattg gcgcgggtca ggttctcgtc caaaaagcgc tcgtagtggc ccaaatcgag
     8821 gtcggtctcg gcgccgtcat ccaggacaaa cacttccccg tgctggaagg ggttcatggt
     8881 accgggatca acgttgatgt atgggtccat cttcaggaga gtcacagtaa ggcctctgca
     8941 ttccatcaac gcgcctatgc tggccgcgga cagcc
//
LOCUS       DBNGZD010000299         5195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1781888, whole genome shotgun sequence.
ACCESSION   DBNGZD010000299 DBNGZD010000000
VERSION     DBNGZD010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1781888"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1040
                     /locus_tag="AC16GM_07995"
     CDS             117..1040
                     /locus_tag="AC16GM_07995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR2923653.1"
                     /translation="MNRQIKTTDVGLSLTEGQLAAQQVALAELLSAFYYQSREYSPPA
                     GLLFIPPEQALREAEVRAGTARLARALHQQVQLVASQKGELSLNRSLLEARQKELLSL
                     KRETERTERQRRQDLEKKGQLLAEIRAQKQETASQVAELEKSAEALEKILAKIEKRRR
                     ARPKPEARPAWQLPAGEFARHKGSLPWPVAGAVVSTFGPKKDPVKKTVSHQPGIDIAA
                     GAGAQVRAIAAGVVAYVGFLRGYDRFVILEHDAGYYTLYAHLEGVQVTEGQVVQSGQT
                     IGSVAGEKNDALLHFEIRQGKQQLNPQEWLQ"
     gene            1028..2140
                     /gene="holB"
                     /locus_tag="AC16GM_08000"
     CDS             1028..2140
                     /gene="holB"
                     /locus_tag="AC16GM_08000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR2923654.1"
                     /translation="MAAVMDRGKVSGQTRLIELLQRAIAHDRLAHAYLFWGPEGIGKW
                     ALAIELAKVVNCEKRRAEPCGICPACRKIDALSHPDLELLYPLPKSRKSRSDDEEDDA
                     DEESRLFFLSEKRKNPYRIVRFERYKTITMARIRETQSRLALKPFEARKRVLIVADAE
                     TLRSDCANILLKTIEEPPPETLVILTTQNPSLLLPTIVSRCQLLRLSPVPDSTITSFL
                     KEQTDADQESVSRAVALAGGSPGKALEFLDPEIQNLLNQAKAVWSEVSHSEMAALVGL
                     RALVYQLPRDRIFLLLDFWANQICAAWAESFDTPQAARQTALPLVSAQKILSDLEQIR
                     AHLWVNVNASLALGAFLLRAGKLLRSAPATASAASS"
     gene            complement(2115..2432)
                     /locus_tag="AC16GM_08005"
     CDS             complement(2115..2432)
                     /locus_tag="AC16GM_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2923655.1"
                     /translation="MFDKPTANTGSPRNFELHQNVPNPFNPTTAITFYLPTETQVELD
                     IFNLLGQRIVRLVDGKLNAGEHMVSWAGQNEHGQSVASGPYFYRLRTDTGFLQIRKML
                     RLR"
     gene            complement(2454..4184)
                     /locus_tag="AC16GM_08010"
     CDS             complement(2454..4184)
                     /locus_tag="AC16GM_08010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR2923656.1"
                     /translation="MFHGTWVAGVAGAKRDNGIFVAGVASGARIVVKNNTGPAPACTI
                     DVSKSVFQIQKAADSGAAAINMSYGGVGPLNFSIPEASAVAYAYRMGVVNVASSGNEG
                     CICVAYPAGYTGVIAVAATDKVDVDWSHANGGNHLDLAAPGVDIVTTSPAAPGYAILP
                     GTSFSAPQVAGLAALLRSRDNWPTAPSTLAGLPALPTSQRFTNDDIEWLMKFGADDVN
                     FATLPGRDSLLGYGRINIRKTFKLLDDIQAGTLAWIPLRIPGGTAIADGQVILRLPDR
                     PLTIQNATRYKVEREVTSAAVFPNFPLVYPRKSTVGWDSSRTNFEVGWLEVISVNARG
                     WKMRTYVFQDNSTLQWYPTSPANVQFDVVVITPPYFTPDFGLTTKVPGNSNQIQLNWE
                     NAGATNYYVERQESPTSPWVTLDTLPGSQFSYIDGPSLVNPQKGSHTYNYRIRSNTGL
                     TSNESSSKTFPNPPNITSATVHLGPECHTGGEGMMGPVPPPGPNLVPTNQIDISWATD
                     PNQDVPIALYQVRFDHAGTPTYLPATTRLDTTLYPLNFDADYTIYGYAYDQEGNLSRV
                     AGAYTVHTGL"
     gene            complement(4154..4918)
                     /locus_tag="AC16GM_08015"
     CDS             complement(4154..4918)
                     /locus_tag="AC16GM_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923657.1"
                     /translation="MPFTKGISRKICFAIVLATAYWGSRTTELFAQVTQDITREVLVQ
                     FRQPGVITFNTGGNLASYSGVTVHSPSVQAVLDQYQPELITEEGRDENLADTIRISPE
                     GDTVRRPIFANIFRIQLPVGGNLQGLANALDTLIYDVIFAERNGTKGYHVCLPATNPD
                     ELLPQQWHLRNTTQRGGLVGADIKYVEPGIDPCWVAPEATPNTQIWLIDTGIDSAHAD
                     FAGNASGDPVMPPETRSQDAEVTAYRLCFMAPGWPV"
     gene            complement(4836..>5195)
                     /locus_tag="AC16GM_08020"
     CDS             complement(4836..>5195)
                     /locus_tag="AC16GM_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR2923658.1"
                     /translation="GDLILFTAYENWTGLSYLMVIRPDGSGMQKVPNTLGNYPLTDWA
                     PDGKHIVYTDVDTSITRFGIYSISILGCNKTRIAAPTLLLSTFSQGGNNAVHERNFPE
                     NLLRHRSGHRLLGQPDN"
BASE COUNT         1143 a         1419 c         1514 g         1119 t
ORIGIN      
        1 ccctgaccgc tctggagaaa aaagaaggga cactggcggc gcagatggca cacctggagg
       61 agaagttgcg cctgtctgat caatagatca agcgcctcaa gtcccgcctg gcgcggttga
      121 accggcagat taagaccacc gacgtcggcc tttcgctcac ggaaggacaa ctggcggcgc
      181 agcaggtggc gctggcagaa ttactttccg ctttctacta ccagtcgcga gaatactccc
      241 cccccgcggg actgctgttc atacctcccg agcaagcgct gcgggaagcg gaagttcggg
      301 cgggcacggc gcgtctggcg cgggccctcc accagcaggt gcagctggta gccagccaaa
      361 aaggagagct ctccttgaat cgatcgcttc tggaagcccg gcaaaaagaa ttgctttccc
      421 tcaaacggga aacggagcgg accgagcggc agcggcggca agacttggag aaaaaaggac
      481 agcttctggc cgaaattcgc gcgcagaaac aggaaaccgc ctcgcaggtt gcggaactgg
      541 aaaaaagcgc ggaggctttg gagaagattc tggcgaaaat tgaaaagaga aggagagcac
      601 ggcccaaacc cgaggcacgc ccggcgtggc agctgccggc tggcgaattt gcccggcaca
      661 aggggagttt gccctggccc gtggccgggg cggtcgtctc cacttttggg cccaagaaag
      721 atccggtgaa aaagacggtg tcgcaccagc ccggcattga catcgccgcc ggtgcggggg
      781 cacaggtccg ggccattgcg gctggagtcg tggcctacgt cggttttctg cgcggctacg
      841 accgcttcgt gattttggag cacgacgccg gttactacac gctctatgcg cacctcgagg
      901 gggtgcaggt gacggaaggg caggtggtgc agagcggcca gaccatcggg tcggtggccg
      961 gtgaaaaaaa cgacgcactt ttgcattttg aaatccgtca gggaaaacag cagctgaatc
     1021 cccaggagtg gctgcagtaa tggaccgggg aaaagtttca gggcagacgc gcctgattga
     1081 actcctccag cgggcgattg cgcatgaccg tctggcgcac gcctacctct tctgggggcc
     1141 ggaaggcatt ggcaagtggg ctttggccat cgaactggcc aaagtggtca actgcgaaaa
     1201 aagacgggcg gagccgtgcg gcatctgtcc ggcttgccgt aaaattgatg cactgagcca
     1261 tcctgatctg gaactcttgt atcccttgcc gaaaagccgc aaatcgcgca gcgacgatga
     1321 agaggatgac gccgacgagg agagccgtct tttcttcctt tccgaaaaac gcaagaatcc
     1381 ctaccggatc gtccgattcg aacgctataa gacgatcacc atggcgcgga ttcgagagac
     1441 ccagtcccgg ctggcgctca agccgttcga agcgcggaag cgggttttga tcgtagccga
     1501 tgcggaaacc ctgcgctccg actgcgccaa catcctgctc aagacgattg aggagccgcc
     1561 gccggaaacg ctggtcattc tcaccacgca aaatcccagt ttgctcttgc cgacgattgt
     1621 ttctcgctgc cagttgctcc ggcttagccc ggtacctgac agcaccatca cctccttcct
     1681 taaagagcag acggacgcgg atcaggaatc ggtttcccgc gcggtggcgc tggccggggg
     1741 gagtccgggg aaggccttgg aattcctcga tccggaaatt cagaatcttc tcaatcaagc
     1801 gaaggccgtc tggtcggaag tgtcccattc ggaaatggca gccctggtcg ggctccgcgc
     1861 gctggtttat caattgccca gagaccgcat cttcctcttg cttgacttct gggcgaacca
     1921 gatttgtgcc gcgtgggcgg agtctttcga tactccgcaa gccgcgaggc aaacggcatt
     1981 acctctcgtt tcggcgcaga aaatcctctc cgacctggag caaatccgcg cccacctctg
     2041 ggtaaacgtc aacgcttccc tggcgctggg ggcgtttctg ttacgcgccg ggaaattgct
     2101 ccggagcgcg cccgctaccg caagcgcagc atcttcctga tctgcaagaa tcccgtatct
     2161 gtccgcaacc ggtaaaagta cgggccgctg gcgacagact ggccgtgttc gttttgacct
     2221 gcccaggaga ccatgtgttc tcccgcattc aacttgccat ccaccagccg cactatccgc
     2281 tgtccgagga ggttgaaaat atccagttcc acttgggttt cggtcggcaa gtagaacgtg
     2341 atcgccgttg tggggttaaa tggattggga acgttctgat gaagctcgaa gttcctaggg
     2401 ctccccgtgt ttgccgtggg tttgtcgaaa atcgacccgc accccgcggc atcctacaaa
     2461 ccagtgtgaa ccgtatacgc ccccgccact cggcttaaat tcccttcctg gtcataggca
     2521 tagccgtaaa tcgtgtagtc ggcatcgaaa ttcagcgggt acagggtggt gtcaagccgt
     2581 gttgtggctg gcaggtaggt aggagttcct gcgtgatcaa accggacttg atagagagcg
     2641 ataggaacgt cctggttcgg atcggtcgcc cacgaaatgt caatctgatt cgtcggaacg
     2701 aggttcggtc cgggtggagg caccggtccc atcatacctt cgccaccggt atggcattcc
     2761 ggacccaagt gtactgtggc agaggtaata tttggtgggt tgggaaaggt tttggaggaa
     2821 ctttcattac tggtaagccc ggtattggaa cggatccgat agttgtacgt gtggcttcct
     2881 ttctgaggat ttaccaagga cggaccatca atgtaggaaa attgcgaacc cggcaacgta
     2941 tctagtgtta cccagggtga agtaggactc tcctgccgtt cgacgtaata gttggttgcg
     3001 cctgcatttt cccagttcaa ctgtatctgg ttagagttac ccggaacttt cgtggtcagc
     3061 ccgaaatcgg gggtgaaata cggtggcgta attacgacta catcgaactg cacattggcg
     3121 ggcgaagtcg gataccattg gagggtacta ttgtcttgaa aaacgtaggt gcgcattttc
     3181 cagccccggg catttactga gataacttcc aaccagccaa cttcaaaatt cgtcctactc
     3241 gagtcccaac ccacggttga tttgcgcgga tagactagag gaaagttggg gaaaactgca
     3301 gcacttgtca cttccctctc cactttgtag cgggtggcgt tctgaatggt taagggacga
     3361 tccgggaggc gcaggatcac ttggccatct gctatggcag ttccgccggg gatacgtaat
     3421 ggaatccagg ccaatgttcc tgcttgaatg tcgtccagaa gtttgaaggt cttccggatg
     3481 ttgatccgcc cgtagcccag caagctgtcc cgacccggca atgtggcaaa gttgacatca
     3541 tctgcgccga atttcatcaa ccattcgata tcgtcgttag tgaaacgttg actggttgga
     3601 agcgccggta agccagccag ggtggatggt gctgtgggcc aattgtcgcg ggaacgtaag
     3661 agcgccgcca gtccagcgac ctggggcgca gaaaacgagg ttcccggtaa aatggcgtag
     3721 ccaggggccg caggacttgt cgtaactata tcaactcccg gcgctgccag gtcgaggtga
     3781 ttgcctccat tggcatgtga ccaatcgaca tcaactttat ctgtggctgc gacagcaatg
     3841 acgcctgtgt aacctgccgg ataggccaca caaatgcatc cctcatttcc actggaggca
     3901 acgttcacga cgcccattct ataagcatag gccaccgctg aagcttcagg gattgaaaaa
     3961 ttgagtggac cgacacctcc gtaactcata tttattgcgg cggcaccgct gtctgcggcc
     4021 ttctgaatct gaaagacaga cttactgacg tcaatggtgc aggccggagc tggccctgtg
     4081 ttgtttttca ccacgattct cgccccgctg gccacgccag cgacaaagat tccattgtcc
     4141 cgtttggctc cggctacacc ggccacccag gtgccatgaa acacaaacgg tacgccgtca
     4201 cctccgcatc ttggctccgg gtctccggag gcattaccgg gtctccggag gcattaccgg
     4261 caaaatcggc gtgtgcagag tcgataccag tgtctatgag ccagatttga gtatttggtg
     4321 tggcttctgg cgccacccag cagggatcga tcccaggctc gacgtactta atgtccgcac
     4381 caaccaaacc gcctctctgg gtggtgttcc gcaggtgcca ctgctgcggc agcagttcgt
     4441 ccgggttcgt cgcagggaga cagacatggt agcccttggt gccgttccgc tcggcaaaaa
     4501 tcacgtcata gattagcgta tcgagtgcgt tagccaaccc ttgcagattt ccaccgaccg
     4561 gcagttgtat acgaaagatg ttggcgaaaa tcggcctacg gacggtgtcg ccttcgggtg
     4621 aaatgcggat ggtgtcggct aagttttcat ctctcccttc ttcagtaatc agttcgggct
     4681 ggtactggtc gagtactgct tggactgagg gactgtgcac tgttactccc gagtaggaag
     4741 caagatttcc ccccgtattg aaggtaataa caccgggctg gcgaaattgg accagcacct
     4801 cgcgggtaat gtcctgcgtc acctgcgcga agagctcagt tgtccggctg ccccaataag
     4861 cggtggccag aacgatggcg aagcagattt tccgggaaat tcctttcgtg aacggcattg
     4921 tttcctcctt gagagaaggt tgacaggagt aatgtgggtg cagcaatccg ggttttgttg
     4981 caaccgagta ttgaaatcga gtaaattccg aaccgtgtga tactggtatc gacatcggta
     5041 tagactatgt gcttaccatc gggtgcccaa tcagtcaagg ggtagttgcc cagggtattg
     5101 ggcactttct gcatcccgga cccatcggga cgaatcacca ttaaatatga cagtccagtc
     5161 caattctcat aagcggtgaa cagaatcaaa tctcc
//
LOCUS       DBNGZD010000300         4293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1646280, whole genome shotgun sequence.
ACCESSION   DBNGZD010000300 DBNGZD010000000
VERSION     DBNGZD010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1646280"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..179
                     /locus_tag="AC16GM_08025"
     CDS             15..179
                     /locus_tag="AC16GM_08025"
                     /inference="COORDINATES: protein motif:HMM:NF026064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923659.1"
                     /translation="MYATQTGAAPPEFLFMSNEPKLVQVSYLRYLKHQIREAFGFAGC
                     PLKFRLRKSS"
     gene            176..478
                     /locus_tag="AC16GM_08030"
     CDS             176..478
                     /locus_tag="AC16GM_08030"
                     /inference="COORDINATES: protein motif:HMM:NF039800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HIR2923660.1"
                     /translation="MSLISEGISVKRDEVTRLPVAFQWRGRECRIEKILKAWQDWGFG
                     AAAPAKKTWRLRRHRNFYQIRTAEGDEYEMYLDRGAKGEKESWVLLRQVSSGSPAT"
     gene            486..1127
                     /gene="plsY"
                     /locus_tag="AC16GM_08035"
     CDS             486..1127
                     /gene="plsY"
                     /locus_tag="AC16GM_08035"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR2923661.1"
                     /translation="MAKLAGIILAAYVLGALPFSLWLARLWKGIDIRRHGSGNPGATN
                     VFRVLGPVPGIGALLLDAGKGFLAVAVVSHFWSDALPTSQMVTSLLTAVAVVLGHIYS
                     FFLGFKGGKGVAPATGAMLALLPMEAGVGIAIFLFVVAISRYVSLGSLVSSACVALLV
                     GGEKFLLKKSLDPLLVGTCLFLTVVIFYTHRANIRRILAGTEHKVGQKDKQVS"
     gene            1143..2150
                     /locus_tag="AC16GM_08040"
     CDS             1143..2150
                     /locus_tag="AC16GM_08040"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR2923662.1"
                     /translation="MATCAILGAGSWAIAVAGLLDGNGHTVRLWEFDPAEAERLRTTR
                     QQPQKLPGIRLPESVLVSNVPAEVLREAHVVLLALPSHIIREVARRLRDLIPRDALVV
                     NLAKGLEIEPLFRMSEVLRSELPAALQKKIVTLSGPSHAEEVSRQMPTSVVVAGTSPE
                     AVQKTQELFANNFFRVYTNADLIGVELAGSLKNIIAIAAGMVDGLGFGDNTKGALLTR
                     GLAEITRLGEKMGAKPATFAGLAGLGDLVTTCLSRHSRNRYVGEQIGKGKTLQQVLAG
                     MVMVAEGVKTTEAAYRLARRHGVEMPITEQVHCVLFENKSPRAAVQDLMTRTLKAEVW
                     T"
     gene            2289..2630
                     /locus_tag="AC16GM_08045"
     CDS             2289..2630
                     /locus_tag="AC16GM_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR2923663.1"
                     /translation="MAEAPEKLYYSISEVAEMVGVKPYVLRFWEKEFPTLKPKKNNAG
                     NRMYQKKEIEFARQIKELLYERGFTIAGARTNLKTERKEARTNPHHPEATVRLVKEIR
                     KDLSDLLKLLT"
     gene            2670..2743
                     /locus_tag="AC16GM_08050"
     tRNA            2670..2743
                     /locus_tag="AC16GM_08050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2704..2706,aa:Pro,seq:ggg)
     gene            2766..3494
                     /locus_tag="AC16GM_08055"
     CDS             2766..3494
                     /locus_tag="AC16GM_08055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2923664.1"
                     /translation="MIRDWPKKNLPSVAILPPKSGKVAILIPAYNAEKTIGEVVKRCA
                     EYVPVTDVVVVNDGSADGTGAKARASGAVVLMHPANRGKGEALKTAFAYAIEKKYTGV
                     VTLDADLQHYPQYVPNFVQTAQRARADVLVGSRPRTFRNMPFSRVVTNTLTSILISIT
                     SGQVVRDSQSGYRYFTTEALKKLRPQSSRYDLESEMLIQAGRCGLRIAAVPISTVYEG
                     SRSFINPFKDTGRFIRILWRSLWW"
     gene            3610..>4293
                     /gene="surE"
                     /locus_tag="AC16GM_08060"
     CDS             3610..>4293
                     /gene="surE"
                     /locus_tag="AC16GM_08060"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR2923665.1"
                     /translation="MLVLITNDDGIQAFGLRALAKEMSKLGRVLIVAPDREQSATSHA
                     LTLHRPLRLNKLAGNVYSVDGTPTDAVMIAVHGILKHRRPDILLSGINHGPNMGDDVT
                     YSGTVAAAIEGTLLGIPSLAISYALGAVRRNSLALRDFALPARIARTVALRMLKHLLP
                     SGTFLNVNIPWVKNGKLRGCEITRLGSRVFNDIIIAKTDPRGKRYYWIGGEPTWKAKT
                     RSDFSALQKK"
BASE COUNT          923 a         1192 c         1276 g          902 t
ORIGIN      
        1 aacatgttcg cattctgtac gcaacgcaaa ccggcgctgc tccgccggaa ttcctcttca
       61 tgtccaacga gcccaaactg gtgcaagtct cctacctgcg ttacctgaag catcaaatcc
      121 gcgaggcgtt cggctttgcg ggttgccccc tgaaattccg cctgcggaag agttcatgag
      181 tctgatctcg gaaggtattt ccgtaaagcg ggacgaggtc acccgattgc cggtggcctt
      241 tcaatggcgg ggaagagaat gtcggattga aaaaatattg aaagcctggc aggactgggg
      301 ttttggcgca gcagctccgg caaagaagac ttggcgtttg cgccgacacc ggaactttta
      361 tcagatacgc acggcggaag gggacgagta tgagatgtac ctggatcgcg gcgcgaaagg
      421 tgaaaaagag tcgtgggtgc tcctgcgcca ggtgagttca gggtctccgg ctacttgagc
      481 agtcaatggc taaacttgcg ggaataattc tcgctgccta cgttctcggt gctttacctt
      541 tcagtctctg gctggcccgc ctctggaaag ggatcgacat ccgccggcac ggttctggca
      601 atccgggagc aacgaacgtc tttcgggttc tggggccggt cccgggcatc ggcgcacttc
      661 ttttggatgc gggcaaagga tttctggcgg tagcggtggt aagtcatttc tggtcggacg
      721 cgcttcccac cagccaaatg gtaaccagtt tgctcaccgc cgtcgcggtc gtcctgggtc
      781 atatctattc gttctttctc gggttcaaag gcggcaaggg ggtagctccg gccaccgggg
      841 caatgctggc gctcctgccg atggaagccg gcgtcgggat tgcgatcttt cttttcgtag
      901 tcgccatcag ccgctacgtt tccttgggtt ctctggtttc gtcggcctgc gttgccctgc
      961 tcgtcggagg ggaaaagttc cttctcaaaa aatcacttga tccgcttctg gtcggtactt
     1021 gccttttcct gaccgtggtg atattctaca ctcaccgggc caatatccgg cggattctgg
     1081 cgggaacgga acataaagtt gggcaaaaag ataaacaggt ttcttaaagc ggaactgtaa
     1141 gcgtggccac ctgtgcaatt ctgggagccg gctcgtgggc aattgccgtc gccggtttat
     1201 tggatgggaa tggccacacg gtgcggctgt gggaatttga tcctgccgaa gccgaacggc
     1261 tccgcactac tcgccagcag ccgcaaaaac ttcccgggat ccgcctgcct gagagtgttt
     1321 tggtcagcaa tgttccggct gaggtgttgc gggaggcgca cgttgtcctc ttggctttgc
     1381 catctcacat catccgggag gtggcccgcc gcctgcgcga tttaatccct agagacgccc
     1441 tggtggtaaa tttggctaag ggtctggaaa ttgaaccgct attccggatg tccgaggtac
     1501 tgcgttcaga attgcccgct gcattgcaga aaaagatcgt gacgctttcc ggaccaagcc
     1561 atgccgaaga agtcagccga cagatgccca cctcagtcgt ggtggcgggc acgtcacccg
     1621 aagcggtgca gaaaacgcag gaactgttcg cgaacaattt ctttcgcgtc tacaccaatg
     1681 ctgatctgat tggcgtggag ttagccggat cgctgaaaaa tatcatcgcc atcgcggcgg
     1741 ggatggtgga tggcctgggt ttcggcgaca atacgaaagg tgccctactc acccgcggtc
     1801 tggcggagat aacccggctg ggtgagaaaa tgggtgcgaa gcccgcaacg tttgccggtc
     1861 tcgccggtct gggtgacctg gttaccacct gcctctcgcg tcacagccgg aaccggtatg
     1921 ttggtgaaca aatcgggaaa gggaagaccc tccagcaggt tctcgccggc atggtgatgg
     1981 tagccgaggg ggtcaaaacc accgaggcgg cctaccggct ggcgcgccgg cacggagtcg
     2041 aaatgcccat caccgagcag gtacattgcg tgctgttcga gaacaagagt ccccgcgctg
     2101 ccgtgcagga tttgatgaca cgcacgctta aggcggaagt gtggacgtag aagcaggtgg
     2161 tgggtcggag actcgtccac ccccctgtag cgcaggttgg tttctgaaac ctgctcccga
     2221 gaagagcgga atacgtgcct cgacttcgct cggcataaga aaaccattcc gcgccaccaa
     2281 gtgacactat ggctgaagcg ccggaaaaac tctactactc gatcagcgaa gtcgccgaaa
     2341 tggtgggcgt caagccctac gtactgcgtt tctgggaaaa ggaattcccc actctcaagc
     2401 ccaagaagaa taacgccggc aaccggatgt accagaaaaa agaaattgag tttgcccgtc
     2461 agatcaaaga actgctgtac gaacgcggtt tcaccatcgc cggcgcccgc accaatctca
     2521 aaaccgaacg caaggaagcc cgcaccaatc cccaccaccc ggaagccacc gtacggctgg
     2581 tgaaggagat ccgcaaagat ctttcggatt tactcaaact tctgacttga gtttgtctct
     2641 ataaaaccta tcttacccaa agcgaatgtc ggggcgtagc gcagcctggt agcgcactcg
     2701 cttggggtgc gagtggtcgg ccgttcaaat cggctcgccc cgataaagac agtgattagt
     2761 gatgagtgat tagggattgg ccaaagaaaa acctgccgag tgttgccatt ttgccgccaa
     2821 agtcgggaaa agtcgccatc ctcattccgg cgtacaatgc ggagaaaacc ataggggaag
     2881 tggttaaacg ctgtgccgaa tatgttccgg tgacagacgt ggttgttgtc aacgatggct
     2941 cagccgacgg gaccggagcc aaagcgagag cgtccggggc tgtggtgctg atgcacccag
     3001 ccaatcgcgg caagggggag gcgctgaaaa ccgccttcgc ctatgcaatt gaaaagaagt
     3061 ataccggggt cgtcacgctt gatgccgacc tacagcacta tccgcaatac gttcccaact
     3121 tcgtgcagac agcccaacgt gcccgggccg atgttctcgt gggcagccgg ccacgcactt
     3181 tccggaatat gcccttctcg cgggtggtga cgaatacgct gacttcaatt ctgatctcga
     3241 ttaccagtgg ccaggtggtg cgggatagcc agtcgggcta ccgctacttc acgacggaag
     3301 cgttgaaaaa gttacgaccg caaagcagcc ggtatgacct cgaatcagaa atgctcattc
     3361 aagcgggacg gtgcggtctg cggattgcgg cggtgccaat cagcacggtc tacgagggct
     3421 cccggagttt cattaatccg ttcaaggaca ccgggcggtt catccgaatt ctgtggcggt
     3481 cgttgtggtg gtgaagattt ttaggcgagc gaggcgctcg ccctacgcga ggagaaaagc
     3541 attatccgta ggcgcggcgc ctcgccgcgt tcagattagc atgcattgcc gcggtaacgg
     3601 agttttccca tgcttgtctt aatcaccaac gacgacggca tccaggcctt cggcctgcgg
     3661 gcactggcca aagagatgtc gaaattaggg cgcgtcttaa tcgtcgcgcc ggatcgggag
     3721 cagagcgcca ccagccatgc cttgacgctt caccggccgc tgcggctgaa taaactggcc
     3781 ggaaatgtct actccgtgga tggtacgccc accgatgcgg taatgatcgc cgtccacggc
     3841 atcttaaaac accggcggcc cgacattttg ctttccggca tcaaccacgg acccaatatg
     3901 ggcgatgacg tgacctactc cgggacggtg gcggccgcca tcgaaggcac cctcctggga
     3961 attccctcac tggccatttc gtatgcgttg ggcgcagtgc ggcgcaactc gcttgcgctc
     4021 cgcgattttg cactgccggc ccggattgcg cgtactgtgg ccctgcggat gctcaagcat
     4081 ctgttgcctt cgggaacgtt tttgaacgtc aacatcccct gggtaaagaa cggcaagctc
     4141 aggggctgcg agattacccg cctgggctcc cgggtgttca acgacattat catcgccaag
     4201 accgacccgc gcggcaagcg ctactactgg atcggcgggg agcctacctg gaaggcgaaa
     4261 acccgaagcg acttctccgc tctgcagaag aag
//
LOCUS       DBNGZD010000301         1788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1438437, whole genome shotgun sequence.
ACCESSION   DBNGZD010000301 DBNGZD010000000
VERSION     DBNGZD010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1438437"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..366)
                     /locus_tag="AC16GM_08065"
     CDS             complement(49..366)
                     /locus_tag="AC16GM_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR2923666.1"
                     /translation="MAEYSVAKVDDVPPGRAKVFQAGEHQIVLCNVAGQFYAIQDRCT
                     HDDGPLGDGELWDEIIECPRHGAQFNVKTGEVVALPAVLPIATYPVKVEGNDIKVRVN
                     GSE"
     gene            complement(441..>1788)
                     /gene="sufD"
                     /locus_tag="AC16GM_08070"
     CDS             complement(441..>1788)
                     /gene="sufD"
                     /locus_tag="AC16GM_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR2923667.1"
                     /translation="TQTAPEKKESAPAVEFSLSSQVVEALSRRTQEPEWLRHRRLEAL
                     ALFEKQPFPKPTDEDWRRTDFSRLDLNAIGIGTNGRPQTPQVPPDASLFLNKPEAFAG
                     RLLSADGNVTARSLQKELQAKGVIFKELKQAVREHPDLLQKYFMTQAVKAADGKFLSL
                     HQALVSSGFVLYVPRGVEVALPLHAFFWSDGHQTSQFPHTLVVAEPDSRVILIDEFAS
                     VTRPAPSFCDNVAELFVGRNAQVVFVHLQRWGENVTNLYKQRAVLEENAQLVTLSVNL
                     GAAYSRIEVESELVAPGASSLMFGLSVGAGKQQIEHHTLQEHVAHHTTSDLLFKTALL
                     DQAYSVFSGLIRIKKDAQRTDAYQANRNLILSPKAKADTLPKLEILANDVRCTHGATV
                     GPIDEEQCFYLMSRGLPRTDTERIVVNGFFEEVLKRVPVGNLKEQLLEYIDRKVLV"
BASE COUNT          363 a          506 c          514 g          405 t
ORIGIN      
        1 cgggagagaa tcgggaaatc ctggcggatt ctatcgacgt ttaacatttc actcgcttcc
       61 attcacacgg actttaatat cgttcccttc aactttcacc ggataggtgg caatcggcag
      121 caccgccggc agggccacta cttctccggt tttgacgttg aactgcgcgc cgtggcgggg
      181 acattcgatg atctcgtccc agagttcgcc atctcccagc gggccgtcgt catgcgtaca
      241 gcggtcctga atggcgtaga attggcccgc gacgttgcag agaacgatct ggtgttctcc
      301 ggcctggaag actttggctc gaccgggggg gacgtcgtca actttcgcta cactgtattc
      361 agccatcacc gatcctgaca atactgcgcg actggttcga cttcgctcac cacaagtaaa
      421 gtcgcgcttc ccagaatttt ttatactaat actttccgat cgatgtattc cagaagttgt
      481 tctttcaaat tacccaccgg gacgcgcttg aggacttctt caaagaaacc gttcaccacg
      541 atgcgctcgg tgtcggtacg cggcaggccc cggctcatca ggtagaagca ctgttcctcg
      601 tcaatcggcc ccaccgtggc gccgtgggtg cagcgcacat cgttggccag aatctccaac
      661 ttgggtagcg tgtcggcctt ggcttttggc gagaggatca gattgcggtt ggcctgatag
      721 gcatcggtcc tctgggcgtc ttttttgatc cggatcaagc cgctgaaaac cgagtaggcc
      781 tgatccaaaa gcgcggtttt gaacaacagg tcgctcgtgg tatggtgcgc cacgtgctcc
      841 tggagcgtgt ggtgctcgat ctgctgtttg cccgcgccga ccgaaagccc gaacatcaaa
      901 ctggaagcgc ccggtgcgac caattcggat tccacttcaa tccttgagta agccgcaccg
      961 agattgaccg agagcgtcac cagttgggca ttctcttcca agaccgcacg ctgcttgtag
     1021 agattggtca cattctcgcc ccagcgctga aggtggacga aaaccacctg cgcattccgg
     1081 ccgacgaaaa gttccgcaac attgtcgcag aaagacggtg ccggcctggt cacggaagca
     1141 aactcatcaa tcaaaatcac tcggctgtca ggttcagcca cgaccagggt gtgtggaaat
     1201 tggctggtct gatggccgtc actccagaaa aaggcgtgca ggggcagcgc tacctcgaca
     1261 ccgcgcggta cgtagaggac aaaacccgag gataccaagg cctggtggag ggagaggaat
     1321 ttgccgtcgg cggccttgac cgcctgggtc atgaaatact tctgcaggag gtcgggatgt
     1381 tcgcgcaccg cctgtttcaa ttccttgaaa ataactccct tggcctgaag ttctttttgc
     1441 agacttctcg cggttacatt gccgtcggct gagagcagtc ttccggcgaa ggcctccggt
     1501 ttattcagga aaagtgaggc gtccggcgga acttgcgggg tctggggtct gccattcgtc
     1561 ccgatgccaa tggcattcaa atcgaggcgg gagaaatcgg tgcggcgcca gtcctcgtcg
     1621 gtcggtttgg ggaagggctg cttttcaaag agcgccagcg cctccagccg ccggtggcgc
     1681 agccattccg gctcctgcgt ccggcgggaa agtgcttcca ccacctgcga agaaagcgaa
     1741 aactctacag ccggtgcgga ttcttttttc tctggcgctg tttgggtc
//
LOCUS       DBNGZD010000302         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1507497, whole genome shotgun sequence.
ACCESSION   DBNGZD010000302 DBNGZD010000000
VERSION     DBNGZD010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1507497"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1073
                     /locus_tag="AC16GM_08075"
     CDS             330..1073
                     /locus_tag="AC16GM_08075"
                     /inference="COORDINATES: protein motif:HMM:NF021554.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HIR2923668.1"
                     /translation="MGWSEIGRNHKNRLFWLGAGEGTAGILAYHQAIQSWGESSGRFH
                     IKNDWLGDGLAGNDEVSHLWWSYEIARAAALSLRWSGMEPKRAQIYGALQSALIMTLV
                     EFPLDAYNPEQGLGISDLVFDYTGVALAVAKARSQALQNFDVKISFRGNPWAENHHFF
                     SQNARDFDRILYWITYRPEKLARGIMIVGLGYQTDHLPPDYRVTRAFVIGLGTTLPDL
                     LRPIAPGLARRMAPLGIYFLNLRCSAVRW"
     gene            1287..>2424
                     /locus_tag="AC16GM_08080"
     CDS             1287..>2424
                     /locus_tag="AC16GM_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HIR2923669.1"
                     /translation="MQFQRYGILAFVGVVTAALFLSTAAPSFGVSASPEVKERLRKAG
                     ATAELQRLLNVEHLEALISSEGADPSKGYRLRPSTLAGITATDTIRVLFVLVDFPDRP
                     ASAGAVTTPTMFDSLLLSTGHKNPTGSYTEFYMENTNGKLFIKGDVIGWYRMDSTNAY
                     YCNGASGLAPNGRARQLARDVALKVDPDVDFTLYDNDGNGTVDGFYVVHAGIGAEETG
                     SGNDLWSHVSNISPSVPVDGKTVSRYTVCPEENSGNRMVAIGVFCHESGHLLFFLPDL
                     YVDSNPGGEGVGEWSLMGSCNWLNNSKTPCHMDPWCKFQRRWAEIETVTTNQNAVSIP
                     KTVGTNKAYRLWTNGSLGTSYFYVENRRRTGFDAALNGEGLLIWQ"
BASE COUNT          501 a          712 c          679 g          532 t
ORIGIN      
        1 gagtccaatc aggacaaggc aacgaatcac tctctcggtc atcttcccct cctgggatga
       61 aatccctttc tctcgagtaa aggccggcca cgcgggcttc actttctcta accaaaaacc
      121 tccgccaaat gttccttgac ctcctgcgga aaatgccttt cctttccctc atgcggcggg
      181 gagtgtgggc tgttctctta ttcctatcca ccgtaggggt gccgactttt tgcgcccaga
      241 cccctctccc cttttccacg gtttcgttct ctctgggaag cctgcagacg cgggatcccc
      301 tcttcgcaac tgattccccg gttccccgaa tgggatggag tgaaatcggg cgaaatcaca
      361 aaaaccgtct cttctggctg ggagcgggcg aaggaaccgc agggattttg gcctaccatc
      421 aggccatcca atcctggggc gagtcttcgg gccggttcca tatcaaaaac gactggctgg
      481 gtgacggtct ggcggggaat gacgaagtct cccacctctg gtggtcctac gagatcgccc
      541 gcgccgccgc tttatccctg cgctggtcgg gtatggagcc caaaagagcc caaatctacg
      601 gcgccctgca aagcgccctg attatgacct tagtggagtt tcccctggat gcttacaacc
      661 cggagcaggg gctgggtata tccgacctcg tgttcgatta caccggtgtt gcgctggcgg
      721 tggccaaggc caggtcgcag gcgctgcaga attttgatgt caaaatctcc tttcggggga
      781 acccctgggc cgaaaaccac catttcttct cgcagaatgc ccgcgatttc gaccgcattc
      841 tgtactggat tacctatcgc ccggagaaac tcgcgcgggg aataatgatt gtcgggctgg
      901 gctaccagac cgaccacctg cctccggact accgggtgac cagggccttc gtcatcggcc
      961 tcggaaccac cctccccgac ctcctccgcc cgattgcgcc cggcctggcc cgccggatgg
     1021 ctccactcgg aatttacttc cttaacctgc gttgcagcgc ggtgcgctgg taaataaaaa
     1081 aagcgcggcc cggcacggcg tttgcctgaa aatacgagaa aaatgggaac aattcttaag
     1141 ctggatgcgg tttatctcat tgacagtcta atgctatggt atatattacc ttttcatcgg
     1201 agctgtacca gcagatgaga cgctttcaga acgagttgtc cgcataacag taccgggaaa
     1261 tgagtaccac aaaaccggag ggtgagatgc agtttcaacg ctacggaatt ttggccttcg
     1321 tcggagtcgt aactgcggcg ctttttcttt ccaccgccgc cccaagtttc ggggtgtcgg
     1381 cctcgccgga ggtaaaggaa aggttgcgga aagccggcgc caccgccgaa ttgcagcgcc
     1441 ttttgaatgt tgagcacttg gaagcgctca tttcttcgga gggcgctgat ccgagtaagg
     1501 gctaccggct gcgccccagc actctggcgg ggataacggc caccgataca atacgtgtgc
     1561 tcttcgttct ggttgatttc ccggataggc ccgccagcgc aggcgccgtc accactccga
     1621 cgatgttcga cagtctgctt ctctccaccg gccacaagaa ccccaccggc agttacaccg
     1681 agttctatat ggagaatacc aacgggaaac tgttcatcaa aggcgacgtc atcgggtggt
     1741 accggatgga tagcacaaat gcctactact gtaacggagc gtcaggtttg gcccctaacg
     1801 ggcgtgcccg acaattagcg cgggacgtgg cactcaaagt agacccggac gtggacttca
     1861 ccctctatga caatgacggg aacggaacag tcgatggctt ctacgtcgtt cacgctggta
     1921 ttggggctga ggaaaccggt agcgggaatg atttgtggtc gcacgtctcc aacatctctc
     1981 cttccgtgcc ggtagacggg aagaccgtct cgcgttacac cgtgtgtccc gaggagaatt
     2041 ccggcaaccg gatggtggcg atcggcgttt tctgccacga gtcgggccat ttgctcttct
     2101 tcctccccga cctgtacgtt gattcgaatc ccggcggcga aggggttggg gaatggtctt
     2161 tgatgggctc ctgcaactgg ctgaacaaca gcaagacgcc ctgccatatg gacccctggt
     2221 gcaagttcca gcgccggtgg gcggagatcg aaacggtcac gaccaatcag aatgcggtgt
     2281 ccattcccaa gaccgtggga accaataaag cttatcggtt gtggacgaac ggctcgctgg
     2341 gcacctccta cttctacgtc gaaaaccgca ggcgcaccgg ctttgacgcg gcgctgaacg
     2401 gcgaggggct tttgatctgg caaa
//
LOCUS       DBNGZD010000303         2736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_813212, whole genome shotgun sequence.
ACCESSION   DBNGZD010000303 DBNGZD010000000
VERSION     DBNGZD010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_813212"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="AC16GM_08085"
     CDS             complement(<1..655)
                     /locus_tag="AC16GM_08085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR2923670.1"
                     /translation="MLVSKQSEYMKTHAAFFCLLVLTVSFFSRVSAQLTDVPSIAEIA
                     ESASQSVVMIIVYDVAGAEKGQGSGFFIDSSGAILTNAHVLKNAYSAEVFSRAGYFAN
                     VTVLSIDKDLDLALIRVSADSAIPVELDFSTDFRAGEQVIAIGNPLGLENTVSDGLIS
                     AIRRIGEDIEFIQTTVPISPGSSGGPLLNSEGKVIGVTTATLGEGQNVNFAIGAKTLR
                     "
     gene            complement(685..2412)
                     /locus_tag="AC16GM_08090"
     CDS             complement(685..2412)
                     /locus_tag="AC16GM_08090"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2923671.1"
                     /translation="MKIYLARHRRQILLGFVCLVVTDILALAWPFILKLAVDSLEAGA
                     SQTRLLLYAGLFFGITAVEGFFRYGMRWILIGVSRRVEYAFRNDFFWHLLTLSRSFYH
                     KTRTGDLLARASNDLDAVRNMLGPGIMYFASTAVTVLVAFVLMFYFSWQLTLAAMIPL
                     PLLSYGVYKISRRVHHYSQKIQEQYATLANMVQENLSGVRVVKAYVQEPHEIGRFRGL
                     NFEFIRRNLRLAKLSALSYPALSSVGGLTMATIIWYGGRRVIAGQLTLGTFVAFTGYL
                     LILIWPMIALGWVIGLYQMGTASLKRIQEIMRTRPEVVSSNGPATVDGRLRGAIAFRN
                     LSFGYAGENHLVLKRVDLEIPPGTTCAIVGATGSGKSTLVNLLPRFFPVKDGQIFVDG
                     GDINQISLDRLRQGIGFVPQEPFLFSDTIAANVAFGSEKVDLEQIKLACEIADFDKDV
                     AGFPKGYDTMIGERGITLSGGQRQRLAIARAVALDPAILILDDAFSSVDTDTEENILK
                     RLRQFGRNRTVILISHRTSTVKDADMIVVLQDGEIGEQGTHEELLVRDGTYAELYRKQ
                     LLEEELERI"
BASE COUNT          691 a          741 c          728 g          576 t
ORIGIN      
        1 ttctaagagt tttagcacct atggcaaagt tcacattttg accctcacct aaggtcgcgg
       61 tcgttactcc gattacttta ccttcagagt ttagtagggg tcccccgcta cttcccggcg
      121 agattggaac cgttgtttga ataaactcaa tgtcttcgcc aattcttcga atggcactaa
      181 tcaatccatc agagaccgta ttttctagtc ctaatggatt cccgattgca ataacttgtt
      241 caccggcacg gaaatctgtg ctaaaatcta attcgacagg aatcgcgcta tctgcggaaa
      301 cacgaattaa agccaagtcc aagtctttat cgatgcttag aactgtaaca tttgcaaaat
      361 aaccagctct ggaaaaaact tcagcggagt atgcattttt gagcacatgg gcatttgtta
      421 ggatagcgcc gctgctatca atgaagaatc cacttccttg acccttttcc gcaccagcga
      481 catcgtacac aattatcata actacacttt gactagctga ttcggctatt tcagcaatgg
      541 aggggacatc tgttaattga gcggatactc gagaaaaaaa agacacagta aggactagaa
      601 gacaaaagaa agctgcgtgt gttttcatat attctgactg ttttgaaacc aacatggaaa
      661 tttgccgccg cttgaataga aagattaaat cctttccagc tcctcttcca acaactgctt
      721 ccggtacaat tccgcatacg ttccatcacg gaccaagagt tcctcgtgtg tcccctgctc
      781 gccaatttcg ccgtcctgca aaaccacgat catatccgcg tctttgaccg ttgaagttcg
      841 gtgagaaatc agaatgacgg tgcggttccg accaaactgc cgcaggcgct tgaggatatt
      901 ctcttcggtg tcggtatcga ccgaggaaaa ggcgtcgtcc aaaatcaaaa tcgccgggtc
      961 cagcgccacc gcccgggcaa tggccagccg ctgcctctgc cccccggaaa gggtaatccc
     1021 ccgttcgccg atcatcgtgt cgtacccttt ggggaacccc gccacgtctt tgtcgaagtc
     1081 cgcaatctcg caggccagct tgatttgttc gaggtcaact ttttccgacc cgaaagcgac
     1141 attggctgca atggtgtcgg agaagagaaa tggctcctgc ggcacaaaac cgatgccctg
     1201 tctcagccgg tcgagtgata tctgattgat atccccaccg tccacaaaaa tctgcccatc
     1261 tttcaccggg aaaaaccgcg gaagcagatt gaccaaggta gacttgcctg accccgtagc
     1321 ccccacaatg gcacaggtcg tcccgggggg aatttctaag tcaacccgct tcaggactaa
     1381 gtggttttcc ccggcgtagc cgaaggagag attccgaaat gcgatcgcac cgcgcaaacg
     1441 tccgtcaact gtcgccggcc cgttggaact gacaacctcg ggccgggtcc gcatgatttc
     1501 ttgaatccgc ttgagcgagg cggtgcccat ctggtagagg ccgatcaccc agcccagggc
     1561 aatcatcggc cagatgagga tgaggaggta gccggtgaac gccacgaacg tgccgagagt
     1621 gagctgaccg gcgattaccc gccggcctcc gtaccagatg atcgtcgcca tggtcaaccc
     1681 gcccacagag gagagcgccg gataggagag ggccgagagc ttggccagcc gcaggttgcg
     1741 ccggataaat tcaaagttca agccccggaa gcgcccgatt tcgtgcggtt cctgcacgta
     1801 ggccttcact acccgcacgc cggaaagatt ttcctgcacc atgttggcca gcgtggcgta
     1861 ctgttcctga attttctgcg aatagtggtg cacccgccgg gaaattttat aaacgccata
     1921 ggaaagcaaa gggagcggga tcatggcggc cagggtcaac tgccaggaga aataaaacat
     1981 taaaacgaaa gccacgagca cggtgacagc ggtggaggcg aagtacataa tccccgggcc
     2041 gagcatgttg cgcaccgcat ccaggtcatt ggaagcgcgg gcaaggaggt cgccggtgcg
     2101 ggttttgtgg tagaatgacc gggagagggt caaaaggtgc cagaagaaat cgttccggaa
     2161 ggcgtactcc acccggcggg aaacgccgat cagaatccag cgcatcccgt aacggaaaaa
     2221 cccctccact gcggtgatgc cgaagaacaa cccggcgtag agcaaaaggc gcgtttggct
     2281 ggcgccggct tccaggctgt ccacggccag tttgagaatg aagggccagg ccagcgccag
     2341 gatgtcggtg accaccagac agacaaatcc cagaagaatc tggcgccggt ggcgggccag
     2401 gtatattttc aagggtgcga gatggtcaaa ggccacattt cacctgaaag ttcggtacac
     2461 accgctccgt cgggcggttt tcctcaagat cgctaaccgg aaacacgggc ccgccgtatg
     2521 gtgagaatat aagggtcagg cggtgctggt aaaagacaac cccttttgac agatgcgcgt
     2581 ggaagcggta aaataagcgc tcgtttgcga tatgagaaca aagtcgcgag gtaaaaaaat
     2641 gcaaaaagta ctttgcaaaa cccaactggt ttggctggca gccggccttt cccttctggc
     2701 caacggaagg tcagccgggg cggcgtctgt tcccgg
//
LOCUS       DBNGZD010000304         5592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_605355, whole genome shotgun sequence.
ACCESSION   DBNGZD010000304 DBNGZD010000000
VERSION     DBNGZD010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_605355"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1671
                     /gene="purH"
                     /locus_tag="AC16GM_08095"
     CDS             94..1671
                     /gene="purH"
                     /locus_tag="AC16GM_08095"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR2923672.1"
                     /translation="MPVAGDAVGIRRALVSVADKTGLQELARVLVSFHVELLSTGGTL
                     AHLRAAGIAAVAVAEYTDAPEILDGRVKTLHPKIQAGILARRDRETDRVEMERLEYPL
                     IDLVVVNLYPFEATIAKENVSSDEAIEQIDIGGPTLVRAAAKNHRFVTVVTSPADYPA
                     LKDELQKNQGSVSLSLRQQFAQRAFAHTAAYDGAITEYLSRQSKTGEIFPEQLSGSFA
                     RVQALRYGENPHQQAALYRKSDYRGPSLATAKLLSGKEPSYNNLMDLDFALSTVLDFS
                     EPCAVIVKHANPCGLACDNSLAQAYQDAYDCDPLSAYGCIIGLNRKVDVETAQRIDAT
                     PFVECVLAPDYAPEVLELLQKKKNRRLVACLEMLQKNALPRRVARVISGGLLVQTPDT
                     YRVGTPDLKTVTQKAPTADQIASLLFAFHAVKQVKSNAIVIAQKTKTVGIGGGVTSRV
                     DAVRLALDKAGDRAKGAVQASDAFFPMPDSIDLAARAGIAAIIQPGGSKADPEVIAAC
                     DQSSIAMVFTGIRHFRH"
     gene            1774..3027
                     /gene="coaBC"
                     /locus_tag="AC16GM_08100"
     CDS             1774..3027
                     /gene="coaBC"
                     /locus_tag="AC16GM_08100"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR2923673.1"
                     /translation="MLTGKKILVGVTGGIAAYKAAYLVRRLRQAGVEVRVVMTKSATQ
                     FITPLTMETLSERPVGIEMFPEREFVGTHHITLADWPELVVVAPCTANVIGKVAGGLA
                     DDLLTNVLIATRAPVLLVPAMHDGMWANPQVQKNIVRLKEEGVHVLDPDIGPLASGDF
                     GPGRLPEVEVILARIEELVGGQGPLKGKKVLVTAGRTEEPLDPVRYLSNRSSGRMGIA
                     LAEAARDTGANVTLVHGPVSIALPAGVTSVSVSSAQEMYAMVADRFDACDVLLMAAAV
                     ADYAPEKPVAHKLKKTKKTLTVTLAPTVDILAEMGREKDKQILVGFALETEQEVKQAR
                     EKLQAKNVDLMVVNNPLEPGAGFEVETNRVTLLFRDGETVPLPLLRKRQVADRIVAEV
                     VNLLKARERKSTQWPKRETRPTYSD"
     gene            2988..3701
                     /locus_tag="AC16GM_08105"
     CDS             2988..3701
                     /locus_tag="AC16GM_08105"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR2923674.1"
                     /translation="MAQERDQANLFGLAQAALKQQTEFGFSEIYFDEALPPAQRFSPT
                     GEAIPGLEAQTLYELYDKIKNCQRCPLGASRTNLVFGSGNPQAAIVFVGEAPGRDEDL
                     QGEPFVGRAGQLLTKIIEAMKMKREDVYIANVLKSRPPENREPLPEEIAACFPYLQKQ
                     IELIKPKFIVCLGRIAAVTLLNTKLPLGKLRGAFHDYQGIKVLVTYHPAALLRFPSYK
                     KDVWDDMKILRKAYDGTVL"
     gene            3820..5193
                     /gene="dnaB"
                     /locus_tag="AC16GM_08110"
     CDS             3820..5193
                     /gene="dnaB"
                     /locus_tag="AC16GM_08110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR2923675.1"
                     /translation="MPDDILSGERLPPQAVEVERQVLAAILLDKTALDRVASLITPDA
                     FYREAHRQIYQAMLDLYEKSEPVDLVTVSEALIKSGQLETIGGRAYLASLLEGVATAA
                     HVEHHAKIVLERYTLRTLAQTATEIVSRCFDPTEEVDELLDRAESRIFSIKEAMVRQS
                     ALPLGEILPHTFESIEEYHKRGGNITGLPTGFTDLDVQTAGLQPADFIVVAGRPSMGK
                     TAFILNVAEHVGCDHQIPLVIFSLEMSKNQLAQRMLCSRGRVSSHAMRTGRLRNEEWA
                     NLSIAVGPLSESKIFIDDTPNMDVLELRAKARRLKTQHKIGLVVIDYMQLMQGPRNAE
                     NRQQEIALISRSLKGLAKELDVPVVALSQLSRAVEVRGGKRRPQLADLRESGAIEQDA
                     DVVMFIYRPKVYDIETTEEGLPTENLAEIIVAKQRNGPTGTVRLSFIPEYARFENMAY
                     VGEQAPF"
     gene            5301..>5592
                     /locus_tag="AC16GM_08115"
     CDS             5301..>5592
                     /locus_tag="AC16GM_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2923676.1"
                     /translation="MTHALRLVGRQTLVILSDVGGLSVLAGRFVLGLPDFFRRFRLTT
                     NQMLAIGVNSLPLILITSTFVGAVSAWQAAYQFKGYVPLRYLGATVGKAVFIE"
BASE COUNT         1214 a         1638 c         1589 g         1151 t
ORIGIN      
        1 accgcccctc ccgcaggaag tggtacggaa tacacaaaag aaatatctcg acgcgtacca
       61 gaaattgacc ggacgaaatc tgtcgctgga gtaatgcccg tcgccggtga tgccgtgggg
      121 atccgccggg cgctggtgag cgtggcggac aagaccggtc tgcaggagtt ggcacgcgtc
      181 ttggtttctt ttcacgtcga actcctttcc accggcggga cgctcgccca cctgcgcgcg
      241 gccggcattg ccgcagtagc agtcgccgag tataccgacg cgcctgaaat tttggacggc
      301 cgggtgaaaa ccctgcaccc caaaattcag gccgggatcc tcgcccggcg cgatcgggaa
      361 accgatcggg tagaaatgga acggctggaa taccccctga ttgatctggt ggtggtcaat
      421 ctctacccct tcgaagccac catcgccaaa gaaaatgttt cttcggacga agccatagag
      481 cagatcgaca tcggcggccc gacgttggtc cgcgcggcgg ccaagaacca ccggttcgtc
      541 acggtcgtca ccagtccggc tgactacccg gcgctcaaag atgaactcca aaaaaatcag
      601 ggatcggtca gcctgtctct gagacagcaa ttcgcgcaga gggcctttgc ccacaccgcc
      661 gcgtacgacg gcgccataac cgagtacctt tcccggcaga gcaaaaccgg cgaaattttt
      721 ccggagcaac tgagcggaag tttcgccaga gtccaggcct tgcgctacgg cgaaaacccc
      781 caccagcagg cggcgctgta ccggaaatct gactaccggg gaccgtcgct ggcgaccgcc
      841 aagcttctct ccggcaagga accttcctac aacaacctta tggacctcga ctttgcactc
      901 tcgactgttc tcgatttcag cgagccctgc gcggtcatcg tcaagcatgc caatccctgc
      961 ggtttggcct gtgataattc gctggcgcaa gcgtatcagg atgcgtacga ttgcgatccg
     1021 ctctcggcgt acggctgtat catcggattg aaccggaaag ttgatgtcga gacagcccaa
     1081 cgaatcgacg ccacgccgtt tgttgagtgt gttctcgccc ccgattatgc tccagaagtg
     1141 ctggaacttt tgcagaaaaa gaaaaatcgc cgtctagttg cttgcctgga gatgcttcag
     1201 aagaatgcac tgcctcgtcg ggttgcacgg gtaatctcag gtggtctttt agttcaaacg
     1261 cctgatacct atcgggtcgg tacgccagac ctaaaaaccg tcacgcagaa agcgccgacg
     1321 gcggatcaga ttgcatcact cctcttcgcc ttccacgcag tcaagcaggt caaatcgaac
     1381 gccatcgtca tcgcgcagaa aaccaaaacg gtcggcatcg gcggcggtgt gacctcccga
     1441 gtcgatgcag tgcggctggc tctggacaaa gccggcgacc gggctaaagg cgcggttcag
     1501 gcgtcagacg ctttctttcc gatgcctgac agcatcgacc tggcagcccg ggccggaatt
     1561 gccgctatca tccagcccgg cggctccaaa gccgacccgg aagtgattgc cgcctgtgat
     1621 caatctagca tcgctatggt cttcaccggc atccggcatt ttcggcactg agggctgtcc
     1681 ccttccggta gatttgtcga tgatgattat gggtctggtt gcactttcac tattttccgt
     1741 tagatttggc gctggttcag gagtaccgtc tcgatgttaa ccggcaaaaa aattctggtg
     1801 ggtgtcaccg gcgggattgc cgcctataaa gcggcctatc tggtgcggcg gctcaggcag
     1861 gcgggagtcg aagttagggt agtgatgacc aaatcggcta cccagttcat cactcccttg
     1921 actatggaaa ctctctccga gcggccggtg ggcattgaga tgttccccga aagagaattt
     1981 gtcggcaccc accacattac actggccgac tggccggaat tggtcgtcgt ggcgccctgt
     2041 acagctaatg ttataggcaa agtggcgggc ggcctggctg atgatctcct cacgaatgtt
     2101 ctcattgcca cccgcgctcc ggtgcttctc gtgcccgcta tgcacgatgg aatgtgggcc
     2161 aatccgcagg ttcagaagaa tattgtccgg ctgaaggaag aaggtgtcca cgttctcgac
     2221 ccggacatcg gccccttggc ctccggcgat tttggcccgg ggcggctgcc cgaagtcgag
     2281 gtcatcctgg ccaggatcga agaattggtt ggcggtcaag ggccgcttaa agggaagaag
     2341 gtgttagtca ccgccgggcg gaccgaagag ccgctcgacc cggtgcgcta cctctcgaac
     2401 cgttcctcgg gtcggatggg cattgccctg gctgaagcgg cgcgcgacac cggggctaat
     2461 gtaaccctcg ttcacggccc cgtttccatt gctttgccag ctggagtgac ctccgtatcc
     2521 gtgagcagcg cgcaagaaat gtatgctatg gtcgccgatc gattcgatgc ctgtgacgtg
     2581 ctcttgatgg ccgccgcggt ggccgactac gcgccggaaa agcccgttgc gcataagttg
     2641 aagaaaacca agaaaacttt aaccgttacg ctggccccga cggtcgatat tttggctgag
     2701 atgggcaggg agaaggacaa gcaaattctg gtcggctttg cgctggaaac cgaacaggaa
     2761 gtcaaacagg cgcgcgagaa actgcaagcc aaaaacgtcg atctcatggt cgtcaacaat
     2821 cccctggagc cgggcgcggg atttgaagtc gaaaccaacc gcgtcacttt acttttccgc
     2881 gatggcgaga ccgtgccctt gcccctttta cgcaaacgtc aggtagccga ccggattgtc
     2941 gccgaagtgg taaacctcct taaagctagg gaacggaagt cgacccagtg gcccaagaga
     3001 gagaccaggc caacctattc ggattagcgc aggccgcgct caagcagcaa accgaatttg
     3061 ggttttcgga aatttacttc gacgaggcgc ttccgccggc gcagcgcttt tccccgacgg
     3121 gtgaggcgat tcccggactg gaagcgcaga cgctctatga gttgtatgac aaaatcaaaa
     3181 attgtcaaag gtgcccttta ggcgccagcc gaacgaactt agttttcggc tccggcaatc
     3241 cccaggccgc aattgtgttc gtcggcgaag cacccggccg ggacgaagac ctgcagggcg
     3301 agccgttcgt cggccgggcc gggcagctgc tcaccaaaat catcgaagcg atgaaaatga
     3361 aacgggagga tgtctatata gccaacgtgc tcaaaagccg gccgccggag aaccgcgaac
     3421 ccctgcccga ggaaatcgct gcctgctttc cgtatctgca gaagcaaatt gaactgatca
     3481 agccaaagtt catcgtctgc ctgggccgga ttgcggcggt gacgcttttg aacaccaaac
     3541 tcccgctggg caaactgcgc ggtgctttcc acgattacca aggtataaaa gtgcttgtca
     3601 cctaccatcc ggcggcgctt ctgcgttttc cctcgtacaa aaaagatgtt tgggacgata
     3661 tgaagatact acggaaggca tatgacggga ctgtgttgta aaattcggat gcacggttaa
     3721 tattgtagcc acgctctctt caggctgagc gaagccgaag gccagagcgt ggagagaacg
     3781 gtagaaaaac gaaatcattg attaaaatcg atatttccga tgccagacga tattctttcc
     3841 ggtgaacgcc ttccgcccca ggcagtcgag gtcgaacgcc aggtcttagc cgcgatcctg
     3901 ctggataaga ctgcccttga ccgggtggcg agtctcatca ctcccgatgc cttctaccgt
     3961 gaagcacacc ggcaaatcta ccaggccatg ctcgatcttt acgagaaaag tgagccggtt
     4021 gatctggtta ccgtctccga agccttaatc aagtcggggc aactggaaac catcggcggc
     4081 cgagcctacc tcgcctccct cctcgaagga gtggccacgg cagcccacgt ggagcaccat
     4141 gccaaaatcg tcttagagcg ctacaccctg cgcacgctgg cccagaccgc caccgaaatc
     4201 gtctcccgct gctttgaccc caccgaagag gtggatgaac tcttagaccg ggccgaatca
     4261 cgcatcttca gcatcaagga ggcgatggtg cggcagagcg ctctgccttt gggcgagatt
     4321 ttgccccaca ccttcgaatc tattgaggaa taccacaagc gaggcggaaa catcaccggt
     4381 ctgccaaccg gctttaccga cttagacgtg caaaccgcag ggctccagcc ggccgatttc
     4441 atcgtggtgg cgggccggcc ctcaatgggg aagaccgctt tcattctgaa cgtcgccgag
     4501 cacgtgggtt gcgaccacca gattccactg gtcatcttca gtctggagat gtccaaaaac
     4561 cagttagccc agcggatgct ctgttcccgg ggccgggttt ccagccacgc catgcgcacc
     4621 gggcggctgc gcaacgagga atgggccaac ctctcgattg cggtcgggcc gctttccgaa
     4681 tcgaaaatct ttatcgacga cacgccgaat atggacgtgc tggaactgcg cgccaaagcc
     4741 cggcggctca agacccagca caagatcggc ctcgtggtca ttgactatat gcaactgatg
     4801 caggggccca ggaacgccga gaaccggcag caggaaatcg ccttgatctc ccgctcgctc
     4861 aaaggactgg ccaaggaatt agacgtgccg gtggtggcgc tttcgcagct ctcccgcgcg
     4921 gtggaggtgc ggggcggcaa gcgccggccg caactggctg atttacgcga atcgggcgcc
     4981 atcgagcagg atgcggacgt ggtgatgttc atttatcgtc cgaaggtgta cgatatcgaa
     5041 accaccgaag agggtttacc gaccgaaaac ttggccgaaa tcatcgtggc caagcagcgt
     5101 aacgggccca ccggtacggt gcggctctcc ttcatccccg agtacgcgcg atttgagaat
     5161 atggcgtacg tgggcgagca ggcgcctttt tgaaaacttt agagaaaaga ttatccctca
     5221 attctaagtt cacaattctg aaacctgtct tccctgattt ttcttttgtg ctttcgcttt
     5281 tctccttgta aactaccggc atgacccacg ccctgcgcct ggtaggccgc cagacgttag
     5341 tcatcctgtc ggacgtcggc gggttgtcgg tcctggccgg gcgctttgtt ctgggtttgc
     5401 ccgatttttt ccgccgtttc cgcctgacga ccaatcaaat gctcgccatc ggggtcaact
     5461 cactgccgct catcctcatt acctccacct tcgtcggcgc ggtctccgcc tggcaggccg
     5521 cttatcagtt caaggggtac gtgccgttgc gctacctggg ggccaccgtc ggcaaagcag
     5581 tcttcattga ac
//
LOCUS       DBNGZD010000305         1951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_883530, whole genome shotgun sequence.
ACCESSION   DBNGZD010000305 DBNGZD010000000
VERSION     DBNGZD010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_883530"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1571)
                     /locus_tag="AC16GM_08120"
     CDS             complement(255..1571)
                     /locus_tag="AC16GM_08120"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR2923677.1"
                     /translation="MPPAKSAEAKSREALTVTDNRTGKTYELPIQDGAIRATDLRQIK
                     VSEDDFGLLAYDPAFMNTACCKSKISFIDGDRGILRYRGYPIEQLAEESNYLETAYLV
                     LYGELPSKAQYDEWVWNITHHTIIHENIKKFIDGFHHDAHPMGMVVSVVAALSTFYPD
                     SKNISSAEVRRKQTYRLIGKMPTLAAFAYRHSKGLFFAYPDNEFSYTGNFLNMMFKMT
                     EVKYKPQPVLEKALDLLFILHADHEQNCSANAMRSVGSSYVDPYCATAAAAAALYGPL
                     HGGANEAVLRMLDEIGSKDKVPEFIRRVKGGEGRLMGFGHRIYKNYDPRAKIIKTMAD
                     QVFEVTGKNPLLDIALELERIALEDEYFVKRKLYPNVDFYSGLIYQAMGFPVDMFPVL
                     FAIPRTSGWIAQWEEMLSDPEQKIARPRQLYLGNVKRDFVPMGKRV"
BASE COUNT          417 a          545 c          521 g          468 t
ORIGIN      
        1 tctcccaaca gtatttcccg ctctacctga aagagatgga gttccggtac aaccaccgca
       61 aagagcccat gttcgaacga ttggcacaac taatggcaaa tcccgtgtca aatcttttat
      121 aatcaccatg ttttttgttg cgtccgattt tcaaacctga cgcacgtggt aagtcttaag
      181 acctgacaca acccccgaaa tgcaaggcgc gggaaagtta cctcccgcgc cttttcttta
      241 cccggctact tatttcacac gcgcttgccc attggcacga aatcgcgctt cacgttccct
      301 aagtagagct gccgcggccg ggcgatcttc tgttccgggt cggagagcat ctcttcccac
      361 tgggcgatcc acccagaagt gcggggaatg gcaaagagca ccgggaacat atccaccggg
      421 aaacccatcg cctggtaaat cagcccggag tagaaatcga cgttggggta gagtttccgc
      481 ttgacgaaat actcgtcctc cagcgcaatc cgctccaatt ccagggcaat gtccaagagc
      541 ggatttttgc cggtgacctc gaaaacttgg tccgccattg tcttgatgat tttggcgcgc
      601 gggtcgtagt ttttgtaaat ccggtgcccg aaccccatca ggcgcccctc gccccctttc
      661 acccgccgga tgaattccgg aactttgtcc ttggaaccaa tctcgtccag catgcgcaaa
      721 accgcctcat tcgccccgcc gtggagagga ccgtagagcg ccgcagcggc tgctgcggtg
      781 gcgcaatagg gatccacgta cgaactgccg acgctgcgca tggcattggc cgaacagttc
      841 tgctcatggt ccgcgtggag gatgaaaagc aagtccaagg ccttctccag caccggctgc
      901 ggtttatact tcacctccgt catcttgaac atcatgttca agaaattacc tgtgtaggag
      961 aattcattgt ccgggtaggc gaagaagagt cccttcgaat gccggtaggc aaaggcggcc
     1021 agagtcggca ttttgccaat cagccgatag gtctgcttcc gccgcacctc agcggaggag
     1081 atattcttgg agtcagggta gaaagttgaa agagcggcca ccaccgaaac caccatcccc
     1141 atcgggtggg cgtcatggtg gaatccgtca atgaattttt tgatgttctc gtggatgatg
     1201 gtgtggtggg tgatgttcca gacccattcg tcatactggg ctttgctcgg caactcaccg
     1261 tagagaacca ggtaagcggt ttccaggtag ttggactctt ccgccagttg ttcaatcggg
     1321 tacccccggt agcgcaggat gcctctatcc ccgtcgatga agctgatctt gctcttgcag
     1381 caggcggtgt tcatgaaggc cgggtcgtac gccagcagcc caaagtcgtc ctcggaaact
     1441 ttgatctgcc gcaggtcggt ggcccggatg gcaccgtcct gaatcgggag ttcgtaggtt
     1501 ttgccggtcc gattatccgt caccgtgagc gcctcacggg atttcgcttc tgcggatttg
     1561 gccggtggca ttggctcttc agccgtcccg cctctggcgg gggtcggctt tggcggggtg
     1621 gctgcggttg ttttcgtcat cggcgtttgc tcctcttcgt ttatcggaaa aaatctcctc
     1681 ctctactatc ccgcaagaat ctacgtagcg gcggggcaaa atacaagact ttttagtatt
     1741 gaatgccacc cggaaaagtc tttgttcttt tggcgcttac gactttcttt aaccactgac
     1801 agcccggtga gaaaaaccgt ctccctcact ggatggatga agttgctccg gacagagatg
     1861 cccataagta gttatggacg tgataattac cgcgataagc aaaggtgata ctttaatatg
     1921 cgccagacgg agaacctatc cccactgtgg c
//
LOCUS       DBNGZD010000306         5714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_953039, whole genome shotgun sequence.
ACCESSION   DBNGZD010000306 DBNGZD010000000
VERSION     DBNGZD010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_953039"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="AC16GM_08125"
     CDS             complement(<1..1930)
                     /locus_tag="AC16GM_08125"
                     /inference="COORDINATES: protein motif:HMM:NF048512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR-like domain-containing protein"
                     /protein_id="HIR2923678.1"
                     /translation="MLPCRCRNAIPTRIALILLLVAGETLAFTSGALAHLSTTFELDG
                     NAIEQAAVSGDDWSLLFSGGGTPLVYSGLLRDSPNDTNRFTGGSKDILDINQWAWDTN
                     PVPDKDEITNGQAALYSGGLLYYQADRFAISGDAQLGFWFLQNTITQNPNGTFSGLHA
                     IGDVLLLSHFTNGGGSVTIAAFRWVGSGGNASDSHFDSIPVSPSIAFAIVNPHDTTSP
                     WPYIPKSGTPGIFPTGAFFEGGLDLLALLGSAAPCFNTFILESRASASLTAELKDFVL
                     GGFRTFPPCSISNPGPFCPGSTNTHCGPPALASYLWRISGNGSISGDSTLQCVNVIAG
                     STCNASYTLTLIVTNAIGCSSTCNLTVNVSDTTRPVITCPANQTIDCAAPVVFGTPTA
                     TDNCANPVITVVSTVGRAGACPQESSFTRTWRATDACGNFAECSQTITKDDNTPPTIS
                     CPQDQTIDCAAAVVFGTPTATDNCDPNPTIRIVSTVGSPGRCPQESTFTRTWEAVDAC
                     GNVSSRCSQTITKRDNTPPVITCAAGKTIDCAAAVVFDPPTATDNCDPNPVITIVSTV
                     GAPGRCPQESSFTRTWRATDACGNFSECSQTITKRDNTPPVVTCPSDRTFECSAIGDF
                     GVATAVDNCDPNPVLTFSD"
     gene            complement(2829..4430)
                     /locus_tag="AC16GM_08130"
     CDS             complement(2829..4430)
                     /locus_tag="AC16GM_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2923679.1"
                     /translation="MHFGLRFPLRFCASVGTLLVLLSANPPAFGQLSLLSQKDGAALD
                     DRLGWAVAGVGDVNGDGVADYLVGAPYTDPGGVATAGSALVYSGATGTLLFQKDGTTT
                     GDRFGTSVAGTGDLNGDGRADFIVGSLSTAFVYSGATGTLLYQKTGGSAVAGAGDVDG
                     DGRADFIVGVAGADPGGVTDAGSVFVYSGATGNLLYQKNGSATGDNLGAAVAGVGDLN
                     GDGRADFIVGAPGFDPGGLFNAGSAFVYSGATGALLYQKNGALTVDLFGSSVAGAGDI
                     NGDGVPDFLIGARLADVGGLTDAGSAFVYSGATGSLLYQKNGTAVGHGLGRSVAGAGD
                     VDGDGRVDFLVGTSDADPGGLTDAGSAFLYSGASGSMLFGANGGAAYDNLGFAIAGVG
                     DLNGDRKAEFLLGVPFADPGGRFDAGSALVYGLRTTNVTRENFSGIPSHFTLAQNFPN
                     PFNAATQIIFDLSGVETRPVRLEIFDILGQEIVKLVDQPLSGGRYRIAWDGRNGQGMP
                     VASGVYLYRLSAGDRQITQRMTLIK"
     gene            complement(4501..>5714)
                     /locus_tag="AC16GM_08135"
     CDS             complement(4501..>5714)
                     /locus_tag="AC16GM_08135"
                     /inference="COORDINATES: protein motif:HMM:NF040218.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HIR2923680.1"
                     /translation="PGSYNDTLVVSGNASNSPQRVPVSYAVSAPPTPAVISLSPTSFA
                     FTAVQNGSLPSSQNLQITNSGGGSLNWSVVKARNSSWLSASPASGTAPSTVVVSITTT
                     GLAPGSYNDTLVVSGNASNSPQRVPVTYTVSAAPSLKGQITQSTATCGSFVSGTSSDL
                     TQVCYVVKNGKISNPDPNVFYYYSQVRAASASFAIDIVQTNNNAAFPFFGVVRNGVAL
                     YDANCTKIGAGTSPSPGQAQVNVTGATVGQLYVISVKYSTNAVAGSPVGLGHPIVHYD
                     YATRVNGVEVTRDPDGSNLAACATPTSVAGTNDVPTLIALQGNHPNPFNASTAIRFSL
                     QQDAHVRLDIYNLLGQKVVTLVDEPRTAGEQTVVWDGKDALGSTVASGVYFYRIAAEN
                     HFEVGRMTLLK"
BASE COUNT         1213 a         1641 c         1693 g         1167 t
ORIGIN      
        1 cgtcgctgaa cgttaagacc gggttcggat cacagttgtc cactgccgtg gctaccccaa
       61 agtctccaat cgcactgcac tcaaaggtgc ggtccgacgg acaggttacc accggcgggg
      121 tgttatcccg cttggtaatg gtctggctgc attccgagaa attcccgcag gcatcggtgg
      181 cccgccaggt tcgggtgaag gaggactcct gcggacagcg tcccggagca cccacggtag
      241 agacgatggt gatgaccggg ttgggatcgc agttgtccgt agcggtcgga ggatcaaata
      301 ccaccgccgc agcacagtca atcgtcttgc ccgccgcaca ggtaatcacc ggcggggtgt
      361 tgtcccgctt ggtgatggtc tggctacagc gtgaagagac gttgccgcaa gcatcgaccg
      421 cttcccaggt acgggtgaaa gtcgactcct gcggacagcg accaggactc cccaccgttg
      481 acacgattcg gatagtgggg tttgggtcac agttatcggt cgccgtcggc gtgccgaaca
      541 ccactgccgc cgcacagtca atcgtctgat cctgcgggca gctgatggtc ggcggggtgt
      601 tgtcgtcctt ggtgatggtc tggctgcact ccgcgaaatt cccgcaggca tcggtggccc
      661 gccaggtccg cgtgaagctc gactcctgcg gacaggcacc cgcacgaccc accgtcgaaa
      721 cgacagtaat caccgggttc gcacagttgt cggtcgcggt cggcgtcccg aacaccaccg
      781 gcgccgcaca gtcgatcgtc tgattcgccg gacaggtgat cactggtcgt gtggtgtcgc
      841 taacgttaac cgtcagattg caggtgctgc tgcatccaat ggcattcgtg acgatcaaag
      901 tcaaggtgta ggaggcattg caggtgctgc ctgcaatcac attgacgcac tgcaaggtgg
      961 aatctccaga gattgagccg tttccggaga tcctccagag ataggaggcg agtgcaggcg
     1021 ggccacagtg tgtgttagtc gaacccggac agaacggtcc cggattactg atcgagcagg
     1081 gcgggaaggt ccggaatccg cccaggacga agtccttcaa ctcggcggtc agtgacgctg
     1141 aggcacggct ctcgaggatg aaggtgttga agcagggtgc agcagagccg agcagggcga
     1201 gcaggtccag tcccccctca aagaacgccc cggtggggaa aattcccggc gtgccacttt
     1261 tgggtatgta cggccagggg gaggtggtgt cgtgggggtt cacaatggca aaggcgatgg
     1321 aggggctgac cgggatcgag tcaaaatggg agtccgaagc attgccgccc gatccgaccc
     1381 accggaaggc cgcgatggtt accgaaccgc cgccgttcgt gaagtggctc agaagcagga
     1441 cgtccccgat cgcatgcagc ccgctgaagg tcccattcgg gttctgggta atggtgttct
     1501 gcaggaacca gaacccgagc tgggcatccc cggagatggc aaaccggtcg gcctggtagt
     1561 acagcaggcc acctgagtac agagccgcct ggccgttggt gatttcgtct ttgtcgggga
     1621 cgggatttgt gtcccaggcc cactggttga tgtccagaat gtccttggag ccccccgtga
     1681 accggttggt gtcgttgggc gagtccctca gcagcccgga atagaccagg ggcgttccgc
     1741 cgccgctgaa gaggagactc cagtcatccc cggacaccgc cgcctgttcg atggcgttgc
     1801 catcgagttc aaacgtggtg gaaagatggg ccagggcgcc gctggtgaat gcgagcgttt
     1861 cgcccgccac caggagcagg atcagcgcta tcctcgttgg gatagcgttt ctacatctac
     1921 atggaagcac ttttccattc ctccgaagtt actggttgtt gttctgcgtg ttgcgttttc
     1981 ccactgtact tcttccatga agtgcgctcg ttgctttacc cattgccctc ctttctgaga
     2041 ggttccttcc ggcggacgca cggtccgcac tgtcgttctg tcttgtctta ccacgaatcc
     2101 gggacgttcg cccacccaga gagagtgaaa ccacgtagaa agactccctg cgacgcaaca
     2161 ccttttcccc ccacgcaatt acaatgccac aggggttgcg gcactttcag ggtctttggt
     2221 tttttatgat ttcaaaaact tgggtgtttc tgtaacttca aacctcgcta acgttcgtcc
     2281 ttcctgatgg acagttccaa accgggtccg cgcaactgac cattttcagt tgctgtaata
     2341 tttttctcac attaactttc taaaaaagaa atttcctcac caaaaaatag aaaggttgcc
     2401 aaccctcctt ccgcgattca ttttcaaaat ctgcctgaga gtccttcctc ggcctaaatc
     2461 ctgtcataag atagattaag gccgggtttt gtcaagcggt ttttttgatt tttacgaaaa
     2521 attcttggaa tccgactgtg aaaaaaacta ccagcgaggg ctgccggaca gagggggtcc
     2581 aatagtcctc caggagaaat atcctgaatt acaggagcta aggaaagtta tgcttttact
     2641 ggaatagttg aaaggcgaag ctgctaagaa gtagatggcg gataagaact gataagttct
     2701 tgcgttgttg aggatagaaa gaaatcagcc ggacaaatcc tgcagtaggt aaattccctc
     2761 taacttctta tccggtcacc aattgtccgc gtgtagtcgc aggaaaatcg gtctttcctt
     2821 tacttcactt actttattag agtcatccgc tgggttatct gtcgatcccc ggcggacagg
     2881 cggtacaggt agacgccgct ggcaaccggc atcccttgcc cattgcggcc atcccaggca
     2941 attcgatatc ttccgccact gaggggttgg tcaaccagtt tgacgatttc ctgccccaga
     3001 atgtcaaaaa tctccagccg caccggtcgg gtttcgactc cggacaaatc aaaaataatc
     3061 tgggttgcgg cattgaaggg attggggaaa ttctgtgcca gagtgaaatg cgaggggatt
     3121 ccggaaaaat tttccctggt gacgttggtg gttctgagtc catagaccag agcggagccg
     3181 gcgtcgaaac gtccgccggg atcggcaaag gggacgccga ggaggaattc ggctttgcgg
     3241 tcgccgttca gatcacctac cccggctatg gcaaacccca ggttgtcata ggccgcaccg
     3301 ccgttggctc caaaaagcat cgaaccgctg gccccggagt agaggaaggc tgagccggca
     3361 tcggtcaatc cgccgggatc ggcatcggag gttccgacta agaaatcgac ccggccgtca
     3421 ccatccacat cgcccgctcc ggcgaccgag cggcccaagc cgtggccgac cgccgtgccg
     3481 ttcttctggt agaggaggct gccggtggcc ccggagtaga cgaaggccga accggcatcg
     3541 gtgagcccgc ccacgtcggc taatcgcgcg ccgatgagga agtcgggtac accgtcacca
     3601 ttaatatccc ccgccccggc caccgaactg ccgaacagat ccacagtcag ggcaccattc
     3661 ttctgataca ggagggcacc ggtggctccc gagtagacga aagccgaacc ggcattaaag
     3721 agcccgcccg gatcaaaacc gggagcaccc acgatgaaat cggcccggcc gtccccgttc
     3781 agatcaccca ccccggccac cgcagcgccc aggttgtcac ctgtggcgct gccatttttc
     3841 tgatagagta aattacccgt agccccggag tagacgaaga ccgaaccggc atcggttact
     3901 ccgcccgggt cagcacccgc cacaccgaca atgaaatcag cgcggccgtc accatcgaca
     3961 tcaccagctc cggccaccgc cgacccgccg gtcttctggt agagtaaagt cccggtggca
     4021 ccggagtaga cgaaggcggt ggagagcgaa ccgacgatga aatcagcgcg gccgtcaccg
     4081 ttcaggtctc cggttccggc cactgaagtc ccgaagcggt cgccggtggt ggtgccgtct
     4141 ttctggaaaa gcagcgtgcc ggtagcgccg gagtagacga gcgccgaacc ggcagtagcg
     4201 actcctcccg gatcggtata gggcgctcca acgaggtaat cagctacacc gtccccattc
     4261 acgtccccga ctccggctac cgcccagccc aaccggtcat ccagcgctgc tccatctttc
     4321 tgggagagca aactgagctg gccaaaagct ggtggattgg cagaaagcaa aaccagcaat
     4381 gtccccacac tggcacagaa tcgcagagga aaacgcagac caaagtgcat aagacctccg
     4441 ttcacttatg ctattctggt aagcccggcc gctggccggg cttaccagat tgaccgggtc
     4501 ttatttcagc agagtcattc tgccgacctc aaagtgattt tcggcagcta ttctgtaaaa
     4561 gtaaacgccg gaggcaacgg tactcccgag ggcgtctttg ccgtcccaca ccaccgtctg
     4621 ctcgccggcc gtcctgggtt catctaccag ggtcaccact ttctggccca gcaggttgta
     4681 aatgtccagc cgcacgtgag catcctgctg gagtgaaaag cgaatcgcgg tgctggcgtt
     4741 gaatgggttc ggatggttgc cctggagcgc tatcagcgtg ggcacatcat tggttccggc
     4801 taccgaagtg ggggtagcgc acgccgccag gtttgaccca tccgggtctc gggtcacttc
     4861 cacgccattg accctggtgg catagtcata gtggacgatg gggtggccta acccgaccgg
     4921 gctgccggcc actgcgttcg tcgagtattt caccgagatg acgtagagct ggccaactgt
     4981 cgcgccggtg acgttcacct gcgcctgccc ggggctgggc gaagtgcccg cgccaatctt
     5041 ggtacaattg gcgtcgtaca gggcgactcc gttccggacc acgccaaaaa acgggaaggc
     5101 ggcgttgtta ttggtctgga cgatgtcgat ggcgaatgat gcactcgctg ctctcacctg
     5161 gctgtagtag tagaagacgt ttgggtcagg attgctgatc ttgccatttt taaccacata
     5221 gcagacctgt gtcagatcag aggaagtgcc gctcacaaaa cttccacagg tggcggttga
     5281 ttgggtaatt tgacctttca agctgggagc ggccgaaacc gtgtaggtta ccggcacccg
     5341 ctggggcgag ttgctggcgt tgcccgacac tactaaggtg tcattgtagc tgccgggagc
     5401 cagaccggtg gtggtgatgg aaaccaccac cgtgctggga gccgtcccgc tcgccggaga
     5461 ggccgaaagc cagctggaat tgcgggcttt gactacagac cagttcaaac tcccaccccc
     5521 ggagttggta atctgcaaat tctgtgaaga aggcaaggac ccgttctgca cggccgtaaa
     5581 agcaaacgag gtcggcgaaa gggatatgac cgccggagtt ggaggagccg agaccgcgta
     5641 gctcaccggc acccgctggg gcgagttgct ggcgttgccc gacactacta aggtgtcatt
     5701 gtagctgccg ggag
//
LOCUS       DBNGZD010000307         4480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1856153, whole genome shotgun sequence.
ACCESSION   DBNGZD010000307 DBNGZD010000000
VERSION     DBNGZD010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1856153"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="AC16GM_08140"
     CDS             complement(<1..703)
                     /locus_tag="AC16GM_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR2923681.1"
                     /translation="MRFSLGASALVFTLIAQTTFAAPYIQFASRPEDSAFVRQTHQAL
                     LTGVQRLEQFAPGAFPETIFVFVAATPEEFDQYSLFHPPDWGAAVAVPALNRIVLKSP
                     KIAPGGQPLEEIVTHELAHLYLYRLAGRAHLPRWIDEGFASWQSGEWSYGQSLVLARA
                     NLSNSLIPLEEIDEVLKFRPAKAALAYAQSHLAVQYFVDSYGKGAFVELAHGLGKSRR
                     PDSVFFAILGIDRQGY"
     gene            complement(717..3164)
                     /locus_tag="AC16GM_08145"
     CDS             complement(717..3164)
                     /locus_tag="AC16GM_08145"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR2923682.1"
                     /translation="MKRRAKIFLWVIGLLVALLLVGAAVLKLIFTRERILALVLPKVE
                     QAVGRKVTVGDASLSIWGGLGLELSQVTVANRKAFSSPYLATLDGLDVRVRFWPLLRK
                     QLDVTKLILNLPQIFLEKNKKGQTNYAGLFQSRTPEAAAKKTDSLLAVAFDRLEIKEG
                     RVIWRDDSTQTLAALTDLSARLALARVGASGGRLSGQLAAGVQYEKGLKKFSSGRAPI
                     TLEPEVIFDLAREQVTLNSVKLTWGKVALAGSGNLSGWQRPAFDLKFLSSRINVGDLW
                     QTLVTSFPEKLAQAQAAGFLQLDLEVIGRKGEATGPHLSGNLLLENVELKTAETPAPL
                     RIRSGLVDFSPDELRISTQSASFGDSPLKVLATVTDFKDPRLSADLDFNLDLRFLSGL
                     SQAKDKTKFWGTLSGRLLANGKMKQPELLNLSGSVQARDVGYQTKAMAMPVEGLNGTA
                     EIDNQMARIQELSFKSGKSSFTLTGTLVRFVPLLFSKAPGQPKPLFTFRLDAPFFDYD
                     EMYPTRRNAAGKVIISTDTVPLPDIEARGKAAVKRGVYSQVEFTDFTADVHYLNRLLT
                     LDNARMTVYGGGAAGKATVDLHDQLHPQFAIAAKADSIEANGFLTRFSFFRDRLFGRM
                     AFNGTFSSRGLLNDEIVRHLSANGKAQMTGARLVRWPLVTQLGAFLNLNLQEEQKIRD
                     LASRFAVENGKVAFDAVTFATGDGDFALAGSVGLDGSLDYRLTTILSPENSQKFRLSS
                     GLKQLFTTDDGRVVLDLNIGGTATDPAFALDFSRAEKKAQEQIQQKLQEKKEEVGKSL
                     REKGQDLLQGLFKKKKP"
     gene            complement(3219..3899)
                     /locus_tag="AC16GM_08150"
     CDS             complement(3219..3899)
                     /locus_tag="AC16GM_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002015517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR2923683.1"
                     /translation="MAKLTWEGHACFKIETGKHNIWIDPFLSGNPLAKTKPEQVKADF
                     ILVTHGHGDHLGDTIAIAKRTKATVIAPFELAMYCQMHGAPNVHPMHIGGGHHFPFGH
                     VKLTIAHHGSAMVDKEITYTGNPCGFLVTSEGKTIYHAGDTGLFGDMALIGQMQKIKA
                     ALLPIGDNFTMGPDDAVKAVELLKPEIVIPMHYNTFEVINQNPQEFVRKVKGATVVIL
                     EPGKSFEI"
     gene            complement(3920..>4480)
                     /locus_tag="AC16GM_08155"
     CDS             complement(3920..>4480)
                     /locus_tag="AC16GM_08155"
                     /inference="COORDINATES: protein motif:HMM:NF042466.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HIR2923684.1"
                     /translation="VRTVAHRLNLRNADKPDSQEICFVTDNNYQRFLRERLAKAGVAM
                     TPGPIYDTGGRKLGEHQGVAFYTVGQRRGLGVAVGEPLYVVAIDPRENAVIVGKDDEL
                     RRRSFTVSQVNWVSIPEPKEALRCNVRIRYRHREAPATVTVTGSDSVQVEYDVPERAI
                     TPGQSAVFYDGDLVVGGGIIESASGG"
BASE COUNT          944 a         1305 c         1296 g          935 t
ORIGIN      
        1 cgtacccttg gcgatcaatc cccaaaatgg caaagaagac cgaatcgggt cgccgcgatt
       61 ttcccaatcc atgcgccagt tccacgaaag cgcctttgcc gtaggaatcg acgaagtact
      121 gcacggcgag atgactttgt gcgtacgcca gggccgcttt cgcgggtcga aacttgagca
      181 cctcgtcaat ctcctcgaga ggaatgaggg agttcgagag attcgcccgt gccagaacca
      241 aagactgacc gtacgaccac tcgcccgact gccaggaggc gaacccctca tcgatccagc
      301 gcggcaggtg ggcgcgcccg gccaggcggt acaaatacag gtgcgccagt tcgtgggtga
      361 cgatttcctc cagcggctga ccgcccgggg caatcttcgg agatttcagg acgatccggt
      421 tcagagccgg caccgccact gctgcgcccc agtcgggcgg atggaagagt gaatactggt
      481 cgaattcctc cggcgtggcg gcgacaaaga cgaatatcgt ttcgggaaaa gcacccggag
      541 cgaactgttc cagccgctga acgccggtta aaagcgcctg gtgagtctgc cggacaaaag
      601 cggagtcttc tgggcggctg gcaaattgaa tgtatggagc tgcaaaagtc gtttgtgcaa
      661 tcagggtgaa aacgagagca gaggcaccca aagagaatcg caaggaagcc agcccctcac
      721 ggcttctttt tcttaaataa accctggagt aagtcttgac ctttttcgcg caaactcttc
      781 cccacctcct cttttttctc ctgcaacttt tgttgaattt gctcctgagc cttcttctcc
      841 gcccggctga agtccagagc aaacgccgga tcggtcgccg tgccgccgat gttgaggtcc
      901 agcaccaccc gcccgtcgtc ggtggtgaag agctgcttga ggccgctgga gagacggaac
      961 ttttgcgagt tttccggaga gagaatcgtg gtcaaacggt aatcgagcga gccatccagt
     1021 cccaccgacc cggccagggc aaagtcgccg tcgccggtgg cgaaggtaac ggcgtcaaaa
     1081 gccacctttc cattctccac agcgaaccgg cttgccaggt cgcggatttt ctgttcttcc
     1141 tgaagattga gattgaggaa agcgcctaac tgcgtcacga gcggccagcg gaccaatcgt
     1201 gcgccggtca tttgcgcttt gccattggcc gagagatgcc ggacgatttc atcgttcaaa
     1261 agcccccgag aactgaacgt gccgttaaaa gccattcttc caaagagccg gtcccggaaa
     1321 aaggaaaagc gcgtgagaaa accgttggct tcaatggaat cggctttcgc cgcaatggcg
     1381 aactgcggat gcagctggtc gtgcaggtcg actgtggcct tcccggccgc gccgccgccg
     1441 tagacggtca tccgggcgtt gtccaaagtc aaaagtctat tgaggtaatg gacgtcagcg
     1501 gtgaagtcgg tgaattccac ctggctgtaa acgcctcgtt tgaccgctgc cttcccccgc
     1561 gcctcaatgt ccgggagcgg aaccgtatcg gtggaaataa tcactttccc cgccgcattc
     1621 cgccgggtgg ggtacatttc atcgtaatcg aaaaaaggcg cgtcgagcct gaaggtaaaa
     1681 agcggcttcg gctggcccgg tgctttggaa aagagcaaag gcacgaaccg caccagcgtg
     1741 ccggtgagcg taaaagaaga tttgccgctt ttgaaggaga gttcctggat gcgcgccatt
     1801 tgattgtcga tctcggcggt gccgttcagc ccttctacgg gcattgccat cgctttggtc
     1861 tggtagccca cgtcccgggc ctgtaccgag ccggagagat tgagcaactc cggctgtttc
     1921 atttttccat tcgccaacaa tcgaccggaa agggtgcccc agaacttcgt tttgtccttc
     1981 gcctgagaga gaccggaaag gaagcgcagg tcgaggttga agtccagatc cgccgacagg
     2041 cgcgggtctt tgaagtcggt gaccgtcgcc agtactttga gcggtgagtc gccgaaggaa
     2101 gccgactggg tcgaaatccg gagttcgtcc gggctgaagt cgacgagtcc gctccgaata
     2161 cgcaggggcg ccggcgtttc ggcggttttc aattcgacat tttcgagaag cagattgccg
     2221 gataggtgcg ggcctgtggc ttcgcccttg cgtccgatga cctccagatc gagttgcaaa
     2281 aagccggcgg cctgcgcttg ggccaatttt tccggaaaac tcgtgaccag agtctgccac
     2341 agatcgccca cgttgatccg gctggaaagg aacttcaagt cgaaggccgg acgctgccag
     2401 ccggaaagat ttccggagcc ggcgagcgcc accttgcccc aggtgagctt gacgctattc
     2461 agggtgacct gttcccgcgc cagatcgaaa atcacctcgg gctcaagcgt tatgggggcg
     2521 cgaccggagg aaaatttctt tagccctttt tcgtactgca caccggccgc gagctgcccc
     2581 gaaagccgcc cgccggaagc gccgacgcgc gccagcgcca gccgggcgga aagatcggtc
     2641 agggcggcga gcgtttgagt ggaatcatcc cgccagatca cccggccctc cttgatttcg
     2701 agccggtcga aggcaaccgc caaaagggag tcggtctttt tcgccgccgc ttccggcgtt
     2761 cggctctgaa acagaccggc gtaattcgtc tgtccctttt tgttcttttc cagaaaaatc
     2821 tgcgggaggt tgaggattaa cttcgtgacg tcaagttgct ttctcaaaag cggccaaaag
     2881 cgcacgcgca catccaagcc gtcgagtgtc gccaggtacg gactggagaa cgccttccgg
     2941 ttggcaacgg tgacctggct caattccaac cccagcccgc cccaaatcga aagggatgcg
     3001 tcgccgacgg ttactttccg accaacggcc tgttccactt tgggcaacac gagagccaga
     3061 atgcgctctc tggtgaaaat gagtttcagg actgccgcgc ccacaagcag gagggcaacg
     3121 agaagcccga tgacccagag aaaaattttg gctctacgtt tcattgttgg cactcacgaa
     3181 ccccgacccc acactgccga cttcaggtct atcggcaact aaatctcaaa actcttgccc
     3241 ggttccagaa tgacaaccgt cgcgcctttc accttacgga cgaactcctg ggggttctgg
     3301 ttgatgacct cgaaggtatt gtagtgcatg ggaatgacga tttcgggttt aagcagttcc
     3361 acggctttga ccgcatcgtc cgggcccatg gtgaagttgt cgccgatggg caaaagcgcc
     3421 gctttgatct tctgcatttg gccgatgagc gccatatcgc cgaaaagacc ggtgtcgccc
     3481 gcgtgataga tcgttttgcc ttcgctggtg accagaaagc cgcagggatt ccccgtgtag
     3541 gtgatttcct tatcgaccat ggctgagccg tggtgcgcaa tcgtgagttt gacgtggccg
     3601 aagggaaagt ggtgcccgcc gccgatgtgc atcgggtgga cgttgggtgc gccgtgcatc
     3661 tggcagtaca tagccagttc aaaaggcgca atgacggtgg ccttcgttcg cttggcaatg
     3721 gcgatggtgt cgcctaagtg gtcgccgtgc ccgtgggtca ctaagatgaa atcggctttg
     3781 acctgctcgg gtttggtctt ggctaacgga ttgccggaga gaaagggatc aatccagatg
     3841 ttatgcttgc cggtttcgat tttgaaacag gcatgtcctt cccacgtcag tttggccatc
     3901 tgccctccat agcgaaaatc tatccgcctg aggcggattc gataatccca ccgcctacca
     3961 ccaagtcacc atcgtaaaaa accgccgact ggcccggcgt aatcgcccgc tccggtacgt
     4021 catactccac ctgaacagaa tccgatccgg taacggtaac tgtagccgga gcttcccggt
     4081 gccggtagcg gatgcgtacg ttacagcgga gcgcttcttt gggctccggg atggaaaccc
     4141 agttcacctg cgatacggta aaacttcgcc tccgcaattc atcatctttg ccgacgatga
     4201 ccgcattttc tcttggatca atcgccacca cgtaaagcgg ctctccaact gccactccga
     4261 gtcctcgccg ctgacccacc gtgtagaacg ccacgccctg atgctctccg agctttcgcc
     4321 cgccggtgtc gtaaatcggc cccggggtca tggccacgcc cgccttagcc agccgctcgc
     4381 ggagaaaacg ctggtagttg ttgtccgtaa cgaagcaaat ctcctggctg tccggcttgt
     4441 cggcgttgcg gagattgaga cgatgcgcca ccgtccgaac
//
LOCUS       DBNGZD010000308         3288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1161290, whole genome shotgun sequence.
ACCESSION   DBNGZD010000308 DBNGZD010000000
VERSION     DBNGZD010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1161290"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1101
                     /locus_tag="AC16GM_08160"
     CDS             148..1101
                     /locus_tag="AC16GM_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2923685.1"
                     /translation="MPLDFAIETSDLSKSFDSFTAVDRVTLSVRPGEIFGLVGPDGAG
                     KTTTIRLLVGVLSITSGRARVAGFDLTQEPDEVKKRIGYMSQRFALYGELTVDENIRF
                     FAEIYRVPKAEREALTKRLLDFSKLAPFRTRQARHLSGGMKQKLGLCCALVHTPEVLF
                     LDEPTTGVDPVSRRELWDILYDLLEQKVTIFVSTPYMDEAERCSRVAFLSGGRVLTCD
                     TPSALKKNFPEKVWEVTCSDSQRASELLDGVDFVSGVTVFGERVHVVTPTEENVVQRI
                     SALLAAQGITVQNIRQVSPSLEDVFISLARQKSQVEYGGSS"
     gene            1591..2565
                     /locus_tag="AC16GM_08165"
     CDS             1591..2565
                     /locus_tag="AC16GM_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2923686.1"
                     /translation="MSKANGLAVEVKDLSRRFGSFVAVDRVSFAVERGEVFGFLGPNG
                     AGKTTTIRILCGILVPTSGSATVAGIDVAHKPDLVKEKIGYMSQKFSLYEDLTPAENL
                     DFFSGIYRISKKEREERKRWVLKMSGLVEWANEVTSELPVGFKQRLALGCAILHRPEV
                     VFLDEPTAGVDPASRRVFWKLIAELAAEGTTVFVTTHYLDEAEHCHRLALMNNGRIVA
                     QGSPQELKLSQRDQDFLEIECAEPVRALGLLRAAFPEWEIALFGNFLHVAAPVGDKAC
                     ASVRAFLQKAGVEIYRCEEIVPTLEDIFVTVVRREESVASVTEEKALP"
     gene            2562..>3288
                     /locus_tag="AC16GM_08170"
     CDS             2562..>3288
                     /locus_tag="AC16GM_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2923687.1"
                     /translation="MRSRIPAIMRKEFITVRRDSTVIANVFVLPLVMMLIYGYGIRFD
                     IRELPTAIYDLSRTPESRDLISAVVGSGYFRRVADLTSTAEVDTLFEQSKIKVALVIP
                     PRYATDVGLGKLARVQVLVDGSDANTATLAVGYLEGIIAKHSGSLLGEKFEKAGIHLP
                     FEIPAVTQETRLWYNPEQKSVNFIVPGIIVVIMSLLGALMTTMTIVGEKERGTMEQIV
                     ASPVTSTEFMVGKIVPYMLLAMTA"
BASE COUNT          699 a          866 c         1025 g          698 t
ORIGIN      
        1 agagggtgtc ccgccaggga cgggagaggt cagagaaaat cagtttgata gctcaaggga
       61 actaacataa tttcagcaat gtgccggttt atggtataac ccctccctca gccctcccct
      121 caaggggagg gaaattctct ccaaaccatg cctcttgatt ttgccataga aacctccgac
      181 ctcagcaaat cttttgattc tttcaccgct gttgatcggg tgacgctttc tgtccgtccc
      241 ggagaaatct ttggcctggt ggggccggat ggggcgggga agacgacgac gatccggctt
      301 ttggtcgggg tgctctcgat cacttccggc agggcgcggg tggcgggatt tgatttaacg
      361 caggagccgg atgaagtcaa aaagcggatc gggtatatgt cccagcggtt cgcgctgtac
      421 ggcgagttga cagtggatga gaatatccgt ttctttgcag aaatctatcg cgtcccgaaa
      481 gccgaacgtg aagcgctgac caaacggctg ttagactttt ccaagttagc gcccttccgc
      541 acaaggcagg cgcggcactt atcgggcggg atgaagcaga agttggggct ctgctgcgcc
      601 ctcgtccaca cgccggaggt gctctttttg gacgagccga ctaccggcgt cgatccggtc
      661 tcccgccggg aactgtggga catcctgtac gacctcttag agcagaaggt gacgatcttc
      721 gtctccacgc cttatatgga tgaggcggag cggtgcagcc gggtggcctt tctctccggc
      781 ggcagagttt tgacctgcga cacgccgtca gctctgaaaa agaattttcc ggaaaaggtg
      841 tgggaggtga cctgctccga ctctcaacgc gcctccgaac tgcttgatgg cgtcgatttc
      901 gtcagcgggg tgacggtttt cggcgagcgg gtgcacgtcg tcacgcccac agaggagaac
      961 gtggtgcaga ggatttccgc gcttttagcg gctcagggga ttaccgtcca gaatatccgg
     1021 caggtatcgc cttcgttaga ggatgtgttt atttcgctgg cgaggcagaa gtctcaggtg
     1081 gagtatggcg gcagcagttg accccaaccg ttatccctcc cctccaaggg gagggaacta
     1141 tacctctttt gcagaagcag tagggttgag acgagtttcc ctctcctttg aggagagggt
     1201 gtcccgccaa aggcgggacg ggagaggtca gagtgcctcg tgagagaata gaagttgacc
     1261 cctcccttgt tccctcccct cggaggggag ggtgtccgta cggacgggag aggtcaggag
     1321 acataaaggg aaagaaactg aaacatgagc gcagacaacg gatacgcagt tgaagtttcg
     1381 tagtcgagga ttctatcctc ggcgcccgta cgggcgtggc acttcaaaag ggaacggttc
     1441 cagcgaacca ccgaggttgg tcggtacgac ccgtatggga aacctcggct acgaagctaa
     1501 ttgtggggaa ggttggattc aggtcagtct ccctctcctg taggagaggg tagggtgagg
     1561 tcaaaagcat taaaggaaag atactgaaac atgagcaaag ccaacggact cgcagttgaa
     1621 gtcaaagacc tctcgcgccg cttcggctcg ttcgtggcgg tggatcgggt ttcgttcgcc
     1681 gtcgagcggg gagaagtgtt cggcttcctc ggccccaacg gcgcggggaa gacgacgacc
     1741 atccggatac tgtgcgggat tctggtgccc acctccgggt cggccaccgt cgccggcatt
     1801 gacgtggcgc ataagccgga tttagtcaag gaaaaaatcg gctatatgtc gcagaaattc
     1861 tctctctacg aagacctgac ccctgcagag aatctcgact tcttcagcgg catctaccgc
     1921 atctccaaaa aagagcgcga ggaacgaaaa cgttgggttc tcaaaatgtc cggtttggtc
     1981 gagtgggcga atgaagtaac ctccgaattg cccgtgggat tcaagcagcg tttggctctg
     2041 gggtgcgcca ttctccaccg gccggaagtc gttttcttgg acgagccgac cgccggagtt
     2101 gaccccgctt cccgccgcgt tttctggaaa ctgatcgccg aattggcggc tgaggggacg
     2161 acggtattcg tcaccaccca ctacctggac gaggccgagc actgccaccg gctggctctg
     2221 atgaacaacg gccggattgt ggcgcagggg agcccgcagg aactgaagtt gtcgcagcgc
     2281 gatcaggatt ttctggagat cgaatgcgcg gagccggtgc gggcgctggg actcctgcgc
     2341 gctgcttttc cggagtggga aattgcgctc tttgggaact tcctgcacgt ggccgcgccc
     2401 gtaggggata aagcgtgtgc cagtgtccgg gcgtttttgc aaaaagcagg ggttgagatt
     2461 taccgctgcg aggagatcgt cccgacgctc gaagacattt tcgtcaccgt cgtccgacgg
     2521 gaagagtcgg tagcgtcagt cacagaggag aaagcgctac catgaggtcg cgcattccgg
     2581 ccatcatgcg caaggagttc atcacggtca ggcgcgactc gaccgtcatc gccaacgtct
     2641 tcgttctgcc attggtgatg atgctgattt acggctacgg catccgcttc gatatccgag
     2701 aactgccgac cgcgatttac gacttaagcc gcacgccgga gagccgcgac ctgatttccg
     2761 ccgtggtcgg ctcgggctac ttccggcgag tggccgattt gaccagtacg gcggaagtcg
     2821 acaccctctt tgagcaatca aaaatcaaag tggcgctggt gatcccgccg cgctacgcca
     2881 ccgacgtcgg attggggaaa cttgcgcgag tgcaggtttt agtggatggt tcggatgcga
     2941 acacggcgac tctggcggtg ggatacttag aaggaatcat cgccaaacat tcaggcagtc
     3001 tgttaggcga aaaatttgag aaggcgggca tccacctgcc gtttgagatt cccgccgtca
     3061 cccaagagac gcggctctgg tacaatccgg agcaaaagtc ggtcaacttc atcgtgccag
     3121 gaatcatcgt cgtgattatg agtctcttgg gggcgctcat gaccactatg accatcgtgg
     3181 gcgagaagga gcgggggacg atggaacaga tcgttgcctc gccggtcacc tcaaccgaat
     3241 tcatggtcgg gaaaattgtc ccctatatgc tgctggcgat gacggcct
//
LOCUS       DBNGZD010000309         6470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_883636, whole genome shotgun sequence.
ACCESSION   DBNGZD010000309 DBNGZD010000000
VERSION     DBNGZD010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_883636"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="AC16GM_08175"
     CDS             complement(<1..1376)
                     /locus_tag="AC16GM_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HIR2923688.1"
                     /translation="MTPQPKGYFSLVLHAHLPYVLAHGRWPHGTDWLSEAACETYLPL
                     LNVFNDLVATGIPPKVTLNLSPVLCEQLASHAFVAELLTYLDQKIKAAKQDRPAFKQK
                     GLIQLAATAAMWHDFYLGIKKDFEEKYNHDLIGAFRKLNDTGSIEVITCAATHGYLPL
                     LAQDMSVQAQVKQAVVAHQRHFGKKPRGLWLPECAYRPGYAWAPPVESPLGCTAHPRK
                     GVEEFLSENGIEFFFIDAALLKGGKAIGVYIDRFEALAQLWQQFEKEYRPAEENILRS
                     PREVYLVGLPEKKPVAVFTRDAETALVVWSGEHGYPADGNYLDFHKKHFPGGLRYWKV
                     TSAKSDLADKKEYEPEAVEARLEENANHFVSLVKREILAYYEQTKKPGLVVAPYDAEL
                     FGHWWFEGPRFLKKVLTKIAQDPEISLSTAGEYLDLAKPSTVISIPEGSWGEGGYHYI
                     WLNQWTEWT"
     gene            complement(1561..1902)
                     /locus_tag="AC16GM_08180"
     CDS             complement(1561..1902)
                     /locus_tag="AC16GM_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR2923689.1"
                     /translation="MEIKPQPQQINVELGEKESEGIYSNLAFITHSPSEVIIDFARLL
                     PGVPRSKVYARVIMTPQHAKLLLQTLDENLKKYEAQFGQIKIFGKEDKGIGFKTVEPS
                     ATPVVRTDQPV"
     gene            2154..3113
                     /gene="pfkA"
                     /locus_tag="AC16GM_08185"
     CDS             2154..3113
                     /gene="pfkA"
                     /locus_tag="AC16GM_08185"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR2923690.1"
                     /translation="MKRIGVLTSGGDAPGMNSAIRAVVHEAAEKGLSVFGFSRGFNGL
                     IDNEGIELDLKAVGGLLQRGGTMLLSARSDEFKTPEGRKTARQSLEKRKVDALVVIGG
                     DGSFKGAQLLEEEGVAVAGVPATIDNDIYGTDMAIGVDTALNTIVEVVDKIKDTASSL
                     ERTFVIETMGRDSGYLALMSAIATGAEAAIIPEVPYDLEKIARRLYDRYQRGATNSII
                     IVAEGASSAYEISRKLAKIASIETKITVLGHLQRGGSPSAFDRLLGSRLGTAAVEALL
                     RGESGVMVGLVKRGVETTPLAEVFRHQKTLDKRSIELAEILSK"
     gene            3234..4220
                     /locus_tag="AC16GM_08190"
     CDS             3234..4220
                     /locus_tag="AC16GM_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR2923691.1"
                     /translation="MQFPQEFPLRQIPWGFLKRGTLLVLIIALGGLFLCCGKKKSVTP
                     IGRGHDTIPIPPPPQRLAQNAQDEAWSPRGGFIAYSHVENVFGTEESGLYLINSDGTG
                     ERLLFPGLVYNLTWSPDGEWIAFYYQRQIFKIKLSGDSLTQLTFDQDFQHLYPRWSPD
                     GKWIAYSVPTFPPGGLWVMSSKDGSQRRQLLENGGLSFWISEDEIGYLFAVTDTTTPR
                     MWAGYMAKFNLNDSTLTILDTIGPLTSHPDYNDRTGKVIFANGKNYDYSLRIYMQDLD
                     GKNEKLVLTNHPAYFPAWLPDGGWIVYTNLDSTEGGLWITDTLGQNQRRLFR"
     gene            4409..5431
                     /gene="rfaE1"
                     /locus_tag="AC16GM_08195"
     CDS             4409..5431
                     /gene="rfaE1"
                     /locus_tag="AC16GM_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HIR2923692.1"
                     /translation="MTVPHPGINHLISAKRIRQLFRRFGGRKIIVLGDLMLDEYLWGS
                     VNRISPEAPVPVVEVTGSSVKLGGAANVALNLKALGDQPVVVGVIGADDKGKKLAHEL
                     ERQHLSKRGLVVDKSRPTTTKLRIIAHNQQVVRADRETAEEILDAVAERLLKVLVREL
                     HGAAGLVISDYGKGVITASILRSVIAEARGRGLFLAVDPKETHFNSYRTVTVITPNHH
                     EAAFAYGKRIRDEKTLEEVGRGLLARLDCESVLITRGERGMSLFEKNGRVTHIPTVAK
                     KVFDVTGAGDTVISSFAAAYAAGATLREAAFIANVAAGLVVEELGTAQVAKERLLAEL
                     LNHSRG"
     gene            5655..>6470
                     /locus_tag="AC16GM_08200"
     CDS             5655..>6470
                     /locus_tag="AC16GM_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR2923693.1"
                     /translation="MKALVIPKHGPPEVFIEAERPVPEIKPNEILIRTEAAGVNFADL
                     MGRMGLYPEAPKPPFVPGYEVCGVVEKIGANVAGIAVGTLVMAFSKFNGYAEYVAASA
                     AGVFPLPQNLSPTEGAAVPVNYLTAYVNLITVGNAKSGERILVYGGTGGVGNAMLQLA
                     RGLGVEIFATCGSEEKVAYLKKLGVEHAINYRTSDVLAAVRRLTAGKGVHLVLDPVGG
                     ESIRRDFHMLAPLGRIVCYGASSLAPGSRRNIFTALWQYLRFPRVNPIRLMQRN"
BASE COUNT         1408 a         1705 c         1925 g         1432 t
ORIGIN      
        1 gtccattcgg tccactggtt gagccagatg tagtggtagc cgccctcgcc ccaggaaccc
       61 tccgggatgg agataaccgt tgatggtttg gcgagatcga ggtattcacc ggcggtagag
      121 agggaaatct ccgggtcctg ggcaatcttc gtaagcacct ttttcaaaaa gcgcgggcct
      181 tcgaaccacc agtggccgaa gagttcggca tcgtagggag cgacgaccaa tccgggtttt
      241 ttggtttgtt catagtaagc cagaatctcg cgcttaacca acgaaacgaa gtggttcgca
      301 ttttcttcaa gtcgcgcttc caccgcttcc ggttcatatt cctttttgtc ggccaaatcg
      361 cttttggcgc tggtgacctt ccagtaacgc aggccgcccg ggaaatgttt tttgtggaag
      421 tccagataat tgccgtcggc ggggtagccg tgctcgcccg accagacgac caaagcggtt
      481 tccgcatcgc gggtgaaaac agcgaccggt tttttctccg gtaagccgac gaggtagact
      541 tcacggggcg agcggaggat attttcttcc gccggccggt actccttttc aaactgctgc
      601 cagagctgcg ccaacgcctc aaagcggtcg atgtagaccc cgatggcttt gccgcctttt
      661 agaagcgcgg cgtcgatgaa gaaaaactcg atcccgtttt cactcaaaaa ttcttccacg
      721 cccttgcgcg ggtgggcggt gcaacccaaa ggcgactcaa ccggcggggc ccaggcataa
      781 ccgggacggt aggcgcactc cggcagccag aggccgcggg gctttttgcc gaaatgtctc
      841 tggtgcgcga ccaccgcttg ctttacctgc gcttgcacac tcatatcctg cgcgagaagc
      901 gggaggtaac cgtgtgtcgc agcgcaggtg atcacctcga ttgacccggt gtcgttcagc
      961 ttgcgaaaag caccgatgag gtcatggttg tacttttcct caaaatcttt cttgattccc
     1021 aagtaaaagt catgccacat ggctgccgtc gcggccagtt ggatcaatcc tttctgtttg
     1081 aaggcgggtc ggtcctgctt ggcggctttg attttctggt cgaggtaggt taaaagttcc
     1141 gcaacgaatg catgggaagc gagttgttca cagagaacgg gtgaaagatt gagggtcact
     1201 ttggggggga tgcctgtggc gaccaggtcg ttgaaaacat tcaaaagcgg caggtacgtc
     1261 tcgcaggccg cctcggagag ccaatcagtt ccgtgcggcc agcggccgtg cgccaaaacg
     1321 tacggcaggt gcgcgtgcag gacgagggag aagtaacctt tgggttgagg ggtcaccgga
     1381 tgctttgata aattggaatc ggttggtatg acgctttccc gctaagggcc ggcctcccac
     1441 cacgtaaggt agtcgggctc gtctctctgc ccgacggttc tcctcgaccg gctcgggtcg
     1501 gcacgactct tcgaggacga gcccctacgg gtcgttcgac cggtctacca gagtaaccgc
     1561 tcataccggc tggtcggtcc gaaccacagg ggtggcggag ggttcgaccg ttttgaaacc
     1621 gatgccctta tcttctttcc cgaaaatttt gatctggccg aattgcgctt cgtacttctt
     1681 caagttctcg tccaacgtct gcagcaggag tttggcgtgc tggggcgtca taatcacccg
     1741 ggcgtagact ttggagcgcg gcacgccggg caggagccgg gcgaagtcga taatcacctc
     1801 cgagggcgaa tgggtgatga aagcgaggtt ggaataaatg ccctccgatt ccttttcgcc
     1861 taattcgacg ttaatctgct gcggctgcgg tttaatttcc attttgcctc cggttcgtgg
     1921 gcggggattc tcccgaccgg ctcggagacg agccggtcta ccccgacttg ggtagtcggg
     1981 ctcgtccccg agcccgacag gtctgcgacg tgcaatcgtt tcactccgct tccccgcgta
     2041 gtcggcccgt acgggccgac tgctggtctc atttgtgttt cgttacctca cgaatatatt
     2101 cgtccatcct tgagggtcaa ggtgagaaat tttcgagagc ggagatgtcc aacataaaac
     2161 gcatcggcgt tctgacctcg ggcggggacg cacccggaat gaattcggcc atccgggcgg
     2221 tcgtccacga ggcggcggag aaggggcttt cggttttcgg attttcccgc ggcttcaacg
     2281 ggctgatcga taatgaaggt atcgaactcg atctgaaggc ggtgggcggg ctgctccagc
     2341 gcggggggac gatgctcctt tcggcgcgct cggatgaatt taaaacgccc gaaggccgga
     2401 agaccgcccg ccagagtctg gagaaacgga aagtcgatgc cttagtagtg attggcgggg
     2461 acggatcgtt caaaggcgcg caacttttgg aggaagaagg ggtggcggtt gccggggtgc
     2521 ccgccaccat cgacaacgac atctacggca ccgacatggc catcggcgtc gatacggcgc
     2581 tcaacaccat cgtggaagtg gtggataaaa tcaaagacac tgcctcatcc ttggagcgga
     2641 cctttgtcat cgagacgatg ggccgcgact ccggctacct ggcgttgatg tcggccatcg
     2701 ccaccggcgc ggaagccgcg attattcccg aagtccccta tgatctggaa aaaatcgccc
     2761 ggcgcctgta cgaccgctac cagcggggcg cgaccaacag catcatcatc gtggccgaag
     2821 gggcctccag cgcctacgag atcagccgca agttagccaa gatcgcttcc atcgaaacga
     2881 aaatcaccgt cttgggacac ctgcagcggg gcgggtcacc gtcagctttc gaccgcctcc
     2941 tgggcagccg gctgggcacc gcggcggtgg aagcgctcct gcggggcgag tcgggagtga
     3001 tggtggggct ggtcaagcgg ggagttgaaa cgacgccttt agccgaagtt ttccgccacc
     3061 agaagacctt agacaaacgc tcaattgaat tggcggagat tttgagtaag tagggcatac
     3121 aagactacct ccctcctgga gaaaaaaata ctaaatacat aaatttgtct cccgacaaaa
     3181 cctatgcggg ggtcgtataa taagcggtag aaattcagcc cggggccgtt tgtatgcagt
     3241 ttccgcaaga attccctctt agacaaatac cttggggctt cctcaaaaga gggacgcttt
     3301 tagttttaat tattgcctta gggggcctgt tcctttgttg cgggaaaaaa aagagcgtta
     3361 cgcctatcgg gagagggcac gatactatcc ctataccccc gccgccacaa aggttggccc
     3421 aaaatgcgca agacgaggcc tggtccccgc gtggtggttt tattgcctat tcacatgttg
     3481 aaaatgtttt tggcacagaa gaaagcgggc tttatctcat taattcggac ggaactgggg
     3541 agcgattgct ctttcccggt ttggtttaca acctgacatg gagtccggac ggtgaatgga
     3601 tagcatttta ttaccagaga caaattttca aaataaaact aagtggcgat agcctaaccc
     3661 aactgacctt tgatcaagat tttcaacatc tatacccacg ttggtcacct gacgggaagt
     3721 ggatagcata ctctgtgccc actttcccac ccgggggtct ctgggtaatg tcatctaagg
     3781 atggttcgca gagacgccaa ttactcgaaa acggcggcct ttcgttctgg atttcagagg
     3841 acgagattgg ctatctattt gcagttaccg acaccactac cccgcgtatg tgggcaggat
     3901 acatggccaa gtttaacctc aatgacagta cgctaacgat tctcgatact attggtccac
     3961 ttacttcaca ccctgactat aatgaccgaa ctggcaaagt gatctttgcc aacggaaaga
     4021 actatgacta ttcattgaga atttacatgc aagacttaga tggcaagaat gagaaactgg
     4081 ttctcaccaa ccatccggct tacttccccg cctggttgcc cgacggcggg tggatcgtgt
     4141 acacgaacct cgattccacg gaaggaggat tatggattac ggacaccctg gggcaaaatc
     4201 aacgcaggtt gtttcgctaa aaagcagttc agttccagag tgagtgtaca ttgatagtcg
     4261 gaaatgggaa cagctaaact caagaccaga tgtagaactt aaccgtaatc gaaggggaaa
     4321 gcctagcctg aagcgacctt cgctccgagc gccgcttttt gtttttgtct tgacgcaatt
     4381 ggttgtcttc ggtaggtttc cttttgcaat gacggttccg catccgggaa tcaatcacct
     4441 gatttcagcc aaacgtatcc gtcagctctt tcgccgcttc gggggccgga aaatcatcgt
     4501 gctcggcgac ctgatgttag acgagtacct ctggggaagt gtcaaccgca tttcgccgga
     4561 agcgccggtg ccggtggtgg aggtgaccgg ttccagcgtc aaattgggcg gcgcggcaaa
     4621 tgtggccctg aacctcaagg cgctgggtga ccagcccgtg gtggtcggtg tcattggcgc
     4681 agacgacaag ggcaagaaac tggctcatga actggaacgc cagcatctga gcaagcgtgg
     4741 tttggtggtg gataaaagcc gtcccaccac gaccaaactc cggatcatcg cgcataatca
     4801 gcaggtggtg cgggcagatc gagaaaccgc cgaagagatt ttggacgcgg tcgctgaacg
     4861 tctcctaaaa gtgctcgttc gggaactgca cggagcggcg ggattggtca tttccgacta
     4921 cggcaagggg gtcatcactg catccatttt gcgaagcgtc attgccgaag ccagagggcg
     4981 cggacttttt ctggcagtcg atccgaaaga gacgcacttc aacagttacc gcaccgtgac
     5041 ggtcataacc cccaaccacc acgaggcggc tttcgcctac ggcaaacgca tccgggacga
     5101 gaagacgctg gaggaagtgg ggcggggact tctggcccgg ttggattgcg agtcggtctt
     5161 aatcacgcgc ggggagcggg ggatgtcgct ctttgagaaa aacgggcggg tgacacacat
     5221 cccgactgta gcgaagaaag tcttcgacgt caccggcgcg ggggatacgg tcatctccag
     5281 ttttgctgcg gcctacgccg ccggcgcaac gctcagagag gcggcgttca ttgcgaacgt
     5341 ggcggccggg cttgtggtgg aggagttggg cacggcgcag gtggcgaaag aaagacttct
     5401 ggcggaactt ttgaaccata gtcggggata gcttgtagac gaggattaaa tcctccgcgc
     5461 ccgtttgggc gtggcactgt aagcggcgac gatcctcgta gggaaatgac gagatgacct
     5521 caccccgcct ctctcctaca ggagagggag tatcattccc tccccttgag gggagggtga
     5581 aggaggggta gattttggcc actatccctg atgactgaag tcgcttctac cgtatcgaga
     5641 aaagaattgc ctggatgaaa gccctcgtca tccccaaaca cggcccgcca gaagttttta
     5701 tcgaagccga gcggccggtg cccgaaataa agcccaatga aattttgata cgtaccgagg
     5761 cggcgggggt gaatttcgcc gacctcatgg ggcggatggg cctctacccg gaagcgccga
     5821 agccgccctt tgtcccggga tatgaagtct gcggcgtcgt ggagaagatt ggcgcaaatg
     5881 tcgccggcat tgcagtcgga acgctggtga tggcgttttc aaagttcaac ggctatgcgg
     5941 agtacgtggc ggcttcggcg gcgggcgttt ttccgcttcc ccaaaattta tcgccgaccg
     6001 agggcgcggc ggtgccggtg aactatttga ccgcctacgt caatttgatt acggtaggca
     6061 acgcaaaatc cggcgaacgt atcctggtgt acggtggaac cggcggcgtg ggaaatgcaa
     6121 tgcttcaatt agcccgaggt ttaggcgtcg aaatttttgc cacctgcggt tcggaggaaa
     6181 aagtggccta tctgaaaaaa ttgggagttg agcatgcaat caattaccgc accagtgatg
     6241 tactggctgc ggtgcggcgc ctcaccgcag gcaaaggagt tcatttagtt cttgatccag
     6301 taggtggcga gagcatccgc cgagactttc acatgctggc cccgctgggg cggatcgtct
     6361 gctacggcgc ctcgtctttg gcgcccggca gccggcggaa tatctttact gcgctctggc
     6421 agtatctgcg ctttccgcgg gtcaatccga ttcgtttgat gcagcgtaat
//
LOCUS       DBNGZD010000310        13492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_466943, whole genome shotgun sequence.
ACCESSION   DBNGZD010000310 DBNGZD010000000
VERSION     DBNGZD010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_466943"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="AC16GM_08205"
     CDS             complement(<1..406)
                     /locus_tag="AC16GM_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR2923694.1"
                     /translation="MAEKSAPTTPQVKVRDVKTKVFNNYINGEWVESASGRTFENRNP
                     ANTREVIGLFQQSNAEDVKRAVEAAKNAYEGWRLTPAPHRAEILFRAAELLIRRKEDY
                     SRQMTQEMGKVLKETRGDVQEAIDMTYYMAGEG"
     gene            complement(508..1290)
                     /locus_tag="AC16GM_08210"
     CDS             complement(508..1290)
                     /locus_tag="AC16GM_08210"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2923695.1"
                     /translation="MITWTVLGCASGMPARDRGSSAYLLEVDGRGFLFDTGEGVSQAL
                     LRFGKNPNAVERIFLSHFHPDHVMGLPLFIQMVYLLKRTAPLAIYYPEEGKAGLEALF
                     DLTYLLRNRIPFPLEFHTIGANPVFADGQVTVSAHLNTHLCGYAQAIKDGGYPNKMQC
                     YSYLIEAGGRKIVYSADLGSASDLEPLVEGCDLLITESLHVGFQQLFETAAVKGVKKV
                     LLTHMSDEQFSRPEEFATLGQKKGIKEVSIARDGLTIDLSRG"
     gene            complement(1354..1794)
                     /locus_tag="AC16GM_08215"
     CDS             complement(1354..1794)
                     /locus_tag="AC16GM_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HIR2923696.1"
                     /translation="MAKKKIIIAQKDLDIAFADADPDVRYFLDTISGQIRKINERRTA
                     TGDEEEFSRKELERTGHYIPLPHFSREERKHLIENFAGLQADEAVKDALTACLKQPSP
                     SLALKTLFFDQPELRKGWLLFQGETLRKKIAAILEKEGLELQFV"
     gene            complement(1964..2611)
                     /locus_tag="AC16GM_08220"
     CDS             complement(1964..2611)
                     /locus_tag="AC16GM_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR2923697.1"
                     /translation="MRKAVLSTIAATLLLTGLAWADPTTFYLTSKDRRNLVQFESKAP
                     LETIVGTTGQIEGKLAVDPDNSLSATGSFEVDLTTLKTGIDMRDEHMREKYLETAQYP
                     KATFTLTKITKTAQKKLADLQPVEVTAEGLFSIHGVQKPVTVTGQVTYLKESEQTKVK
                     LPGNLLHIDFTFPLLLTDFGIKVPQMVLLKLDNKQVVKVDAFATTTSPEEMSKRQ"
     gene            complement(2790..3233)
                     /locus_tag="AC16GM_08225"
     CDS             complement(2790..3233)
                     /locus_tag="AC16GM_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923698.1"
                     /translation="MPAFTEKDIKKIDTIIGADETTWEKDHFRLQVTNRPERRRLVLE
                     IFPDAQLGAPRKGAMVVVYTGTAHLQLHNCTGYVASEELGEVTFVGEDSGRLSGLVVE
                     QRAACSLYANVNRKVISSDFTKLGVEVMLSGVALSLAEEVLKSTK"
     gene            complement(3234..3431)
                     /locus_tag="AC16GM_08230"
     CDS             complement(3234..3431)
                     /locus_tag="AC16GM_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR2923699.1"
                     /translation="MFGNIGAQELLLILLIVLLLFGARRIPEIAQGLGKGIREFKKGM
                     RDVQEEMNKPESEPKKPEEKK"
     gene            complement(3448..3714)
                     /locus_tag="AC16GM_08235"
     CDS             complement(3448..3714)
                     /locus_tag="AC16GM_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HIR2923700.1"
                     /translation="MKEREITFLVVALILATIFGGLIGEIVGSFLPEGAAKTLFQKSI
                     EVGFDPQTVKLYAVAFTIGLLIKINFVSVLMVVGVIAYFRWWYL"
     gene            complement(3711..4415)
                     /gene="purQ"
                     /locus_tag="AC16GM_08240"
     CDS             complement(3711..4415)
                     /gene="purQ"
                     /locus_tag="AC16GM_08240"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR2923701.1"
                     /translation="MKFGVVTFPGSNCDYDAFAVLRDVLQQPVEFLWHQEQSLKGCDC
                     IILPGGFSYGDYLRTGAIARFSPIMEKVVSFANEGGLVLGICNGFQVLTEVGLLPGAL
                     LRNAWLRFVCRDVHLRVERTDLPFTCACRPGQVLKVPTAHGDGNYFASQEVLGQLEKQ
                     KQIVLRYCEPDGRITPEANPNGSLANIAGIANERSNVFGLMPHPERAAEALLGSADGA
                     WIFRSLVQHLVQRRAA"
     gene            complement(4412..4690)
                     /gene="purS"
                     /locus_tag="AC16GM_08245"
     CDS             complement(4412..4690)
                     /gene="purS"
                     /locus_tag="AC16GM_08245"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011337254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR2923702.1"
                     /translation="MTVAAASNGKKHFKAVVYVSFKDGVLDPQGSTIKRALGTLGYNG
                     IADVRAGKFFEIALLAKDERAAQTEIEEICRKLLANPVIEKYRYEVRS"
     gene            complement(4687..5493)
                     /gene="pssA"
                     /locus_tag="AC16GM_08250"
     CDS             complement(4687..5493)
                     /gene="pssA"
                     /locus_tag="AC16GM_08250"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR2923703.1"
                     /translation="MKGFRGIFPGVFTMGNLFCGFLSVLSSFDGEARHAAWFILLAAF
                     FDGLDGIVARLSKGTTRFGVELDSLADLVSFGVAPAVVLYSFKLSDLGKWGWILGFVF
                     VMCGAFRLARFNLQAKAEGKDHFVGLPVPAAGTAIAAYTLFCYELWGQLRFPEFLVTM
                     IVAFSGLMVSTVVYEAKPRSLRTVKDKIKFAYLLLGTVAVVLKPRLALFPLFAFYVLY
                     GIFREIYDTVASGLNGRPKPAIPPSVLAEKQKRNRLSSLPPWPGEFKEHK"
     gene            complement(5490..6128)
                     /locus_tag="AC16GM_08255"
     CDS             complement(5490..6128)
                     /locus_tag="AC16GM_08255"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HIR2923704.1"
                     /translation="MAKAGWPFVLTALFLALVIGVAGSARNPAAIAGMIFFGFLALGF
                     AFFFRDPERVIPSESRLILAPADGRVVDIKDEENRFLGATAKRVGIFLSVFDVHVNRV
                     PVSGEVAYREYRPGKKLPANSAFASRENECCEIGLVNGKGKVVVRQIAGVLARRIVCR
                     VKAGDKVQPGERFGMIKFGSRVELVLPPSVRLAVSLRQKVKGGETVIGEFLG"
     gene            complement(6121..7416)
                     /gene="purB"
                     /locus_tag="AC16GM_08260"
     CDS             complement(6121..7416)
                     /gene="purB"
                     /locus_tag="AC16GM_08260"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR2923705.1"
                     /translation="MIARYTRPEMAKLWSEETKFKTWLQIEILACEAQAQLGVIPAEA
                     VPIIKQKARFEVAAIEKIEATVKHDVIAFLTNVALSVGKESRFIHYGLTSSDVVDTAT
                     AYLLVQAGKQIVADLQKAVHTLKRLALRYKRTRMIGRTHGMHAEPTTFGLKLLGWYDE
                     IKRQQVRMERAIEAVAVGKISGAVGNFAGVDPEVERYVCEKLGLKPSVVATQVVSRDR
                     HAEYLGTLALIAATVEKIATEIRHLQRTEVGEVEEPFAAGQKGSSALPHKKNPVTCER
                     LCGLARLVRSQAQVALENIALWHERDISHSSAERIIFPDATTLVDYLLATLDWILAHL
                     VVHPDRMRANLDQTGGLVYSGRVLLALAQNGLTREQAYGIVQSNALKSLRLKRPFREL
                     IARDKRVTRQVGAKKVQEFFDEKPYLKHVDYIFKQVLGG"
     gene            complement(7435..8145)
                     /gene="fsa"
                     /locus_tag="AC16GM_08265"
     CDS             complement(7435..8145)
                     /gene="fsa"
                     /locus_tag="AC16GM_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR2923706.1"
                     /translation="MKLFIDSANIDEIREVASWGILDGVTTNPSLVAKEKAGFEEILR
                     EICRIVNGSISAEVVSVEFDAMMREARQLAKLHKNIVVKIPLIKEGLKAIKACSAEGI
                     RTNVTLVFSANQALLAAKCGASIISPFVGRLDDVGHDGMEVVQQIIEIYDNYDYPTEV
                     LVGSVRHPLHVTRAAQMGADIATCPFKVLDQLMKHPLTDVGLKNFLADWEKARKALPE
                     AAAVKKEKARVPTAVGRG"
     gene            complement(8158..8907)
                     /gene="truA"
                     /locus_tag="AC16GM_08270"
     CDS             complement(8158..8907)
                     /gene="truA"
                     /locus_tag="AC16GM_08270"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR2923707.1"
                     /translation="MSERAGKVRNIKLVLEYDGTGFSGWQRQPKRRTVQEEVEKAIEK
                     ITGEKARLTAAGRTDAGVHARKQVANFFSATRLSRETLQRALNALLPKDVRVTKVAQA
                     LLDFDARRRAKSRTYRYVLVQKPTALARFFTWYVFQPLNLTKMQSATRFLLGRHDFSS
                     FCVREADEKSRRCTVAQARWRKAGAGIVFDITADRFLRGMVRSIVGTLVGVGRGKISA
                     AQFRSILRRRDRRKAGPSAPAHGLCLWDVAY"
     gene            complement(8900..9703)
                     /locus_tag="AC16GM_08275"
     CDS             complement(8900..9703)
                     /locus_tag="AC16GM_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR2923708.1"
                     /translation="MGAFGEQNFAYFGQYQAGEGWFFRLDPRSKLLLVAALILAAFRL
                     TDPLSLGLLAIGLCALFILAQFPVTLLIRQFRTVGWLFAVIFLLYWFFSSGQTLLGAG
                     PIGPSQAGALDGLRWCLKLFVFVFSGLLFTSTTSPLDLVDSLLATARFLGLGFVRRWL
                     EEIGLIFYLAFGFVPLFAREAGEIKKAQMARGASFDGPFYQRLRKSVPLVFPLIVNSF
                     RRAEEKAVALEARGFGLAPEPTQLEPRQFSRNDWWVTVAAVILGVSVVV"
     gene            9859..11097
                     /locus_tag="AC16GM_08280"
     CDS             9859..11097
                     /locus_tag="AC16GM_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2923709.1"
                     /translation="MSFQELPQNFRRNYRLSIANGLFFNAANAFITGTTIIPLFVSRL
                     TASTILIGLAASLETAGWLLPQGLVAAMTLDNVYQKPQYIRVAIVRVIVFAALVTSVF
                     LFGRSYPTLCLVSFFLFFSIYAMGGGSAGVAFMDIVAKTIPHHKRGSLWGWRMALGGS
                     LAAVMGLVIKKLLAAFSFPVNFGLVFACAWVLIVAGLTSFSFVIEPPGVNRLPRLSIR
                     QGFQKFLEIFRRDQNFKMLYYIRIAAGSYIIAAPFYIVYGINHLGYPPEVAGLFLTAQ
                     MIGLVITNPLWAKISNTRSNRLVLFWSAVFSTLPPVVVLLSNLTPLPAALYAAIFFLM
                     GVAEAGLTIGYLNYVLEIAPEPLRPLYTGFLHTIIAPTVVFSALGGLVIQLTSFTALF
                     IIALVIGLGAVVLAKKLAFA"
     gene            complement(11277..12311)
                     /locus_tag="AC16GM_08285"
     CDS             complement(11277..12311)
                     /locus_tag="AC16GM_08285"
                     /inference="COORDINATES: protein motif:HMM:NF037752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HIR2923710.1"
                     /translation="MRRITLFAIAGYLLLIAGAAEEALAAPFVDSVIVVSKTFPSGSQ
                     GVIEVKIHNDSAINSINLPLVIRSETGGAFGRVDSIQYTPAIFPLGMTPSLTRVNGAS
                     PDSMFVLSIDSGGGSAPPAPRRIAYKIFVTNNTNPGTYSLDSNRTSPNQFLKFTHPPT
                     QANVPAFVRGVELIGSVPNAMTLVTTQVTLFKGKESCVTVGGTLCDTTSLRLTIDSVK
                     VQHSGDPDLSCSTAVGATNLVGKTFEPFSCATDSGNGFLRVCQAPTASIGDTGTCRIT
                     FFGTLGVGAAARVVSLSAVVSMAAGSTGATVVPTLTQWGLALFGVLLAGMLGYVYYRR
                     RKRSTSCPVT"
     gene            complement(12315..13337)
                     /locus_tag="AC16GM_08290"
     CDS             complement(12315..13337)
                     /locus_tag="AC16GM_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923711.1"
                     /translation="MRSLTIFAIAGNLLLIAGAAEEVAAGPFVDSVIVVSRVFTPGSA
                     GTIDILIHNDSAINGIALPLVIKSETGGAFGRVDSIKRLPALNIFGAAGPFRIDGASP
                     DSLYILSIDSGGGSIPPGPRRGGYRVFVTNNSLVGTYSLDSNRFSSTQFLRVTHPPTQ
                     VSIPVFIRGVEIIGDALNLAATQVTLFKGRDACVNVGGVICDTTAQTLTLDSVRVQHS
                     GDPDLLCMTAVGATNLVGKTLSAFSCATDSGNGFLRVCQAPTASIGDTGTCRITFFGT
                     LPGEAGAPARVVAFSAAVSMAAGSTGATVVPTLTQWGLAGFGVLLAGMLGYVYFRRRK
                     FAMKGR"
BASE COUNT         2840 a         4180 c         3639 g         2833 t
ORIGIN      
        1 gcccctcccc cgccatgtag taggtcatgt cgattgcttc ctgcacgtcg ccgcgggttt
       61 cttttaaaac tttacccatt tcctgggtca tctggcggga ataatcttct ttccgacgaa
      121 tgagcaattc cgccgcgcgg aaaaggattt cggcccgatg aggcgccggc gtcaatcgcc
      181 agccctcata ggcattcttg gccgcttcca ccgcccgctt cacgtcttcc gcgttggact
      241 gctggaaaag accgatcact tcccgcgtgt tagccggatt gcggttctcg aaggtgcggc
      301 ccgaggccga ctcgacccat tctccgttga tgtagttgtt gaaaactttc gtcttgacat
      361 cccgcacctt cacctgcgga gtggttggag ccgatttttc cgccattttg ttacgaacca
      421 ccgctggttt acttatggtt ttcgtcgcca tattgcctcc attttccaat gtagtatatc
      481 caaaaatagt ttacgtttgg cctttggtca accgcgtgag aggtcaattg tcagcccatc
      541 ccgggcaata ctgacctctt ttatcccctt cttctgacca agcgtagcga attcttccgg
      601 ccggctgaac tgttcatccg acatatgggt caaaagaacc tttttcactc ccttaaccgc
      661 cgctgtttca aagagttgtt ggaaaccaac atggagactt tcggttatca agaggtcgca
      721 accctccaca agcggctcaa gatcagacgc actgcccaaa tcggcagagt agacgatctt
      781 ccggccaccg gcctctatca gatacgaata gcactgcatc ttattggggt atccgccgtc
      841 tttgatcgcc tgagcgtagc cacacaggtg cgtattcaga tgcgctgaga ctgtgacctg
      901 cccgtccgcg aaaacgggat ttgctccgat agtgtgaaac tctagtggaa aaggaatgcg
      961 gttacgcaga aggtaggtga ggtcgaagag tgcttccagt cctgcttttc cctcttccgg
     1021 gtagtaaatg gccaaaggcg ccgtgcgctt gagcaggtag accatctgga tgaagagcgg
     1081 caggcccatc acgtggtcag ggtggaagtg agaaaggaag attcgttcca ccgcgtttgg
     1141 atttttgccg aaacgcaaga gcgcttgtga aacgccctcg ccggtatcga agaggaaacc
     1201 ccgcccatcc acctccaaca aataggccga actcccccgg tctctggcgg gcatccccga
     1261 ggcgcacccg aggacagtcc aggttatcat ggggagttaa tcgtagccga ggtttttaac
     1321 ctcggtgccc ccggcgtcga tcttgattct ctttcaaaca aactgtaatt ccagcccttc
     1381 cttctccaaa atcgctgcta ttttcttccg cagcgtctct ccctgaaaca aaagccaccc
     1441 tttgcgcaat tccggctggt cgaagaagag cgttttgagc gccagggaag gactcggctg
     1501 ctttaaacag gcggtgaggg cgtccttgac ggcttcgtcc gcctgcaaac cggcgaaatt
     1561 ttcaattaag tgcttccgct cttcgcggga gaaatgtggc aacgggatgt agtgccccgt
     1621 gcgttctaat tccttccgcg aaaactcctc ctcatcgcct gtcgccgtcc gccgctcgtt
     1681 gattttacgt atttggccgg aaatggtgtc gaggaagtag cgaacgtccg ggtcagcatc
     1741 ggcgaaagcg atatcaaggt ctttctgagc aatgatgatt ttcttttttg ccaccatgtg
     1801 agtaaaaata gttagaatat tccgcaaggg aaagaccgga gagaattgta gcgacgctct
     1861 ccagagcgtc gcgggttcac gccggtcggg gacccggcca aaaaaatggc gcaggatacg
     1921 aagatcatcc tgcgccacgg agaatgaaat ttacccgcgg ctgctactgc cgcttgctca
     1981 tctcctccgg cgaggtggtg gtggcaaagg cgtccacctt caccacctgc ttgttgtcca
     2041 acttcaagag caccatctgc ggcaccttga tgccgaaatc ggtgagcaag agcgggaaag
     2101 tgaagtcgat gtgcaagagg ttgccgggca atttcacttt ggtctgctcg ctctccttca
     2161 ggtaggtcac ctggccggtg accgtgaccg gcttctggac gccgtggatg gaaaatagcc
     2221 cttcggccgt cacctccacc ggctgcaggt cggccaattt cttctgggcc gtcttggtaa
     2281 ttttggtcag ggtgaaggtt gctttcgggt actgcgccgt ctcgaggtac ttctcgcgca
     2341 tgtgttcatc gcgcatatcg atgccggttt tgagcgtcgt caggtccact tcgaacgagc
     2401 cggtagcgga cagcgagtta tccggatcaa ccgctagctt tccctcaatc tggccggtgg
     2461 tgccgacgat ggtttccaga ggcgccttcg actcgaactg caccaggttg cggcggtctt
     2521 tgctggtcag gtagaaagtg gtcggatcgg cccaagccaa tccggtcaaa aggagagttg
     2581 cggcgatggt ggagagaacg gcttttctca caggtactcc tcgggttacg ggtttgtctt
     2641 gctttttcgt tgctacgttt cgacttgcaa aatcccaaac aaaattgtag cgccactctc
     2701 ttcaacctga gtccgcctca ggcggatcga aggccagagc ggcgggattt gcgccggtca
     2761 ggagaccggc gctacgcatt tattggctgc tactttgttg atttcagaac ttcctcggcc
     2821 aaagacaaag ccacgccgga gagcatcacc tcgacgccca atttggtgaa gtcggaggat
     2881 atcaccttcc ggttgacgtt tgcgtagagc gagcaggcgg cccgctgctc caccaccagc
     2941 ccggagagcc ggccgctgtc ctcgcccaca aaggtcacct cgcccaactc ttcgctcgcg
     3001 acgtagccgg tgcagttgtg cagctgcagg tgggcggtgc cggtgtagac gacgaccatg
     3061 gcgcctttgc gcggcgcgcc caattgggcg tccgggaaaa tttctaagac cagccgccgg
     3121 cgctccggcc ggttggtcac ctgcagccgg aagtggtctt tctcccaggt ggtctcgtcg
     3181 gccccgatga tcgtgtcaat ttttttgatg tctttttctg taaaagccgg catctacttc
     3241 ttttcttcgg gtttcttggg ctcgctctcg ggcttattca tctcctcctg gacgtcccgc
     3301 atccctttct taaactcccg gatccctttg cccaaaccct gggcaatctc cggaatgcgc
     3361 cgggcgccga acagaagcag aacgatcagg agaatgagca agagttcctg agccccgata
     3421 ttgccgaaca agggcatcac acctcctcta caggtaccac cagcgaaaat aggcgatcac
     3481 cccgaccacc atcaagaccg acacgaaatt gatcttgatc aaaagcccga tggtgaaagc
     3541 gaccgcgtac agtttcaccg tctggggatc aaaccccact tcaatgctct tctggaaaag
     3601 cgtcttggcc gccccttcgg gcagaaacga accgacgatt tcgccgatca aaccgccgaa
     3661 gatcgtggcc agaatcaaag ccaccaccaa gaaagtaatt tcccgttctt tcacgctgcc
     3721 cgcctctgta ccagatgctg tacgagcgac cggaaaatcc aagcgccgtc ggcggagccc
     3781 aagagtgctt cggcagcccg ctccgggtgg ggcatgaggc cgaagacgtt gctccgttca
     3841 ttcgcaatgc cggcgatgtt ggcaagcgac ccattcggat tcgcttctgg ggtgatgcgg
     3901 ccgtccggct cacaatagcg caagacaatt tgcttctgct tttcaagttg gccgagaact
     3961 tcctgcgacg caaagtagtt tccgtcgccg tgggcagtgg ggaccttcaa cacctgaccc
     4021 ggccggcagg cgcaggtaaa ggggaggtcg gttcgctcga ctcggagatg aacatcccgg
     4081 cagacgaagc ggagccaggc attgcgcaac agcgcgccgg ggagaagccc aacctcggtc
     4141 aaaacctgaa aaccgttgca aatgcccaaa acgaggccac cctcattggc aaaggaaacg
     4201 actttttcca tgatgggcga aaagcgggcg atggcccccg tgcgcaggta gtcgccgtag
     4261 gaaaagccgc ccggcaggat gatacagtcg cagcccttga gggactgctc ctggtgccag
     4321 agaaactcca ccggctgctg cagaacatcc cggagtacgg cgaaagcgtc gtagtcgcag
     4381 ttggagccgg ggaaagtgac gacgccgaac ttcacgaccg gacctcgtag cggtattttt
     4441 cgatcaccgg attggccaga agtttccggc aaatttcttc gatttccgtc tgggccgctc
     4501 gctcgtcttt ggccaaaagt gcgatttcaa aaaacttccc cgcccgcacg tcggcaatgc
     4561 cgttgtaacc caaggtcccc agcgcccgtt tgatggtcga gccctgggga tcgagcacgc
     4621 cgtccttaaa actcacgtag accaccgctt tgaaatgttt tttgccattg gacgctgccg
     4681 ccacagtcac ttatgctcct tgaactctcc cggccacggc ggcagggagg agagccggtt
     4741 gcgcttttgt ttttccgcca gaaccgacgg cggaatcgcc ggtttcggac ggccgttcaa
     4801 tccgctggcc accgtgtcgt aaatttcacg gaaaataccg tagagaacgt agaacgcaaa
     4861 cagggggaag agcgccagcc gcggcttgag caccacagcc accgtcccca ggagaaggta
     4921 ggcgaacttg attttgtcct taaccgtccg cagggagcgc ggcttggctt catacaccac
     4981 cgtcgaaacc atcaaaccgg aaaaagccac tatcattgtt actaaaaatt ccggaaagcg
     5041 cagctgcccc cagagttcat agcagaagag ggtataggcg gcgatggcgg tcccggccgc
     5101 cggaaccggc aggcccacaa agtggtcttt cccttcggcc ttggcctgaa ggttgaagcg
     5161 tgcgagccgg aacgccccgc acatgacgaa aacaaatccc aaaatccagc cccacttccc
     5221 taaatcgctc aacttgaacg aatagagcac caccgccggc gccacgccga aagaaacgag
     5281 gtcggccagc gagtcgagtt cgaccccgaa ccgggtggtc cccttggaaa gccgggccac
     5341 gatgccgtcc agcccgtcga agaaggccgc caaaagaatg aaccaggccg catgccgcgc
     5401 ttccccgtca aaagatgaga gaaccgagag aaacccgcag aagaggttcc ccatggtgaa
     5461 gacacccggg aaaatcccgc ggaaaccttt catccgagaa actccccgat caccgtttct
     5521 ccccctttca ccttctggcg cagggaaacc gcaagccgta ccgacggcgg cagcaccagt
     5581 tccacccgcg agccgaattt gatcatcccg aaccgctcgc ccggttgcac cttatcgccc
     5641 gctttgaccc ggcagacgat ccgccgcgcc agaactccgg caatctgccg caccacgact
     5701 ttccctttgc cgttcaccag accgatctcg cagcactcat tttcccgcga cgcaaaggcg
     5761 ctattggccg gcaacttctt tccgggccgg tactcccggt aggccacctc tccggaaacg
     5821 ggcacacgat tcacgtgcac gtcgaaaacc gaaaggaaga tcccgactcg tttcgccgtc
     5881 gcgccaagga accggttctc ctcatccttg atatcgacca ctctgccgtc cgccggagcc
     5941 aatatgagtc gtgattccga cggaatgacg cgctccgggt cgcggaagaa aaaggcgaac
     6001 cccaacgcca gaaaaccaaa aaatatcatc cccgcgatgg cggccggatt tctggctgac
     6061 cccgcgacac cgatcacgag cgccaaaaaa agagccgtca ggacaaacgg ccatcccgct
     6121 ttagccaccg agcacctgtt tgaaaatgta atccacgtgt ttcaaatacg gcttttcgtc
     6181 gaaaaactct tgcacttttt tcgcgccgac ctgccgggtc acccgtttat cccgggcgat
     6241 taattcccgg aagggacgct tcagccggag ggacttgagc gcgttcgact ggacgattcc
     6301 atacgcctgc tcgcgggtaa gcccgttctg cgccaaagcc aagagcaccc gccctgagta
     6361 aaccagcccg ccggtttggt ctaagttcgc gcgcatccgg tcgggatgaa cgaccaggtg
     6421 cgcgagaatc caatcgagcg tcgctaaaag gtaatcgacc agcgtggtcg cgtcgggaaa
     6481 gatgattcgt tccgccgaag agtgggagat gtcccgctcg tgccagaggg cgatattctc
     6541 caaagccacc tgcgcctggc tgcgcaccag ccgcgccagc ccgcagagcc tttcgcaggt
     6601 gaccgggttc ttcttgtgcg gcaaggccga actccccttc tgacccgcgg cgaacggttc
     6661 ttcgacttcg cctacttcgg tccgctggag gtggcggatt tcggtggcga ttttttcgac
     6721 cgtggccgcg atcaaagcca gcgtgccgag gtattcggcg tgccggtcgc gggagaccac
     6781 ctgcgtggcg acgaccgaag gtttgagccc caatttttcg cagacgtagc gctcgacttc
     6841 gggatcgacc ccggcgaaat tccccaccgc gccggaaatt ttccccacgg ccaccgcctc
     6901 aatcgctcgc tccatccgca cctgctggcg cttgatctca tcgtaccacc ccagaagttt
     6961 cagcccgaag gtggtcggct cggcgtgcat cccgtgggtg cggccgatca tccgggtgcg
     7021 cttgtagcgg agcgccagac gcttgagcgt gtgaactgct ttctgtaaat cggcgacgat
     7081 ttgctttccc gcttgcacga gcagataggc ggtggcggta tccaccacgt ccgacgacgt
     7141 gaggccgtag tggatgaagc ggctttcctt gcccaccgaa agggcgacgt tagtgaggaa
     7201 agcaatgacg tcgtgcttga ctgtcgcttc gatcttctcg atggcggcca cctcaaagcg
     7261 tgccttttgt ttgataatcg gcaccgcctc cgccggaatg actcccaact gcgcctgcgc
     7321 ctcgcaggcc aggatttcga tttggagcca ggttttgaat ttggtctctt ccgaccagag
     7381 tttggccatc tccggccggg tgtagcgggc aatcataagc gcaagaaccg ctccctatcc
     7441 gcgccccacc gccgtgggaa cacgggcttt ttctttttta accgccgccg cttccggcag
     7501 cgctttgcgc gccttttccc agtcggccag gaaattctta agtcccacgt cggtcagcgg
     7561 gtgtttcatt aactggtcta aaactttgaa cggacaggtg gcgatgtcag cgcccatttg
     7621 cgccgcgcgc gtgacgtgca gcgggtgccg cacgctccct accaacactt cggtggggta
     7681 gtcataattg tcgtaaatct cgatgatctg ctgcaccacc tccatcccgt catggccgac
     7741 atcatccagc cgccccacga acggggaaat gatagaggcc ccgcacttgg cggccaaaag
     7801 cgcctgattg gcggaaaaaa ctaaagtgac gttggtgcgg attccttcgg cgctgcaggc
     7861 cttgatcgct ttcaaccctt cttttatcaa tggaattttc accacgatat ttttgtggag
     7921 tttcgccagc tgccgcgcct cccgcatcat ggcgtcgaac tccactgaca ccacttcggc
     7981 ggagatggag ccgttcacga tccggcagat ttcccggaga atctcttcga agccggcttt
     8041 ctccttggct accagagagg gattggtggt aacgccgtcc agaatgcccc aggaggccac
     8101 ctcccggatt tcgtctatat tggcgctgtc gatgaatagc ttcatcttgc ttcctcccta
     8161 ataagctacg tcccagaggc agagaccgtg agccggcgcc gatggcccgg ctttccgccg
     8221 atcgcgccta cggagaattg aacggaattg agcggccgag atcttccccc ggccgacgcc
     8281 caccaaagtc cccacgatgc tccgcaccat cccccgcagg aaccggtcgg cggtgatgtc
     8341 gaaaacaatc cccgctcccg ctttgcgcca gcgtgcctgc gcaaccgtgc agcgccgcga
     8401 cttttcatcc gcttcgcgga cgcaaaaact ggaaaagtcg tgtcgtccca aaagaaaacg
     8461 agtggccgac tgcattttcg tcaaattgag cggctggaaa acgtaccacg taaaaaaccg
     8521 cgctaaagca gtcggcttct gtacgagaac gtatcgatag gtgcggcttt tcgcccttct
     8581 ccgggcgtca aaatcaagca gtgcttgagc cactttcgtc acccgcacat ccttcggcaa
     8641 aagcgcattg agagcccgct ggagcgtttc ccggctgagc cgggtggccg aaaagaagtt
     8701 cgccacttgt ttgcgggcgt gcaccccggc gtcggttcga ccggcggcgg tcaggcgcgc
     8761 cttctccccg gtgatttttt caatcgcctt ttccacttcc tcctgcaccg ttcgtctttt
     8821 gggttgcctc tgccagccgg aaaagccggt tccgtcgtac tctaacacca gtttgatatt
     8881 ccgaactttg cccgctcgct cagacaacca cgcttactcc gagtatgacc gccgcaactg
     8941 tcacccacca gtcgttccgg gaaaactgcc gcggctccaa ctgcgtcggc tccggtgcga
     9001 ggccaaatcc ccgcgcttct aaagccaccg ccttctcctc ggcccggcgg aacgaattca
     9061 cgatcaacgg gaacaccaag ggcacacttt tccgcaagcg ttggtagaac ggcccgtcga
     9121 atgacgcgcc ccgcgccatc tgcgctttct tgatttcacc cgcctcccgg gcaaagagcg
     9181 gcacgaagcc gaaggctaag taaaaaatca agccaatctc ctccagccac ctgcggacga
     9241 aacccagacc cagaaaccgc gccgtggcca agagagaatc aaccaaatcc agcggagacg
     9301 tggtgctggt gaatagcagt ccggaaaaaa cgaagacgaa gagtttcagg caccagcgga
     9361 gcccgtccag tgcgccggct tgcgaaggac cgatgggccc ggcgccgagg agcgtctgcc
     9421 cggaggaaaa gaaccagtac aaaagaaaga taaccgcgaa cagccaaccg acggttctga
     9481 actgcctgat gagcaacgtc acgggaaatt gcgccaggat aaacagggcg cagagcccga
     9541 tcgccagaag gcccagactg agcggatcgg tgaggcgaaa cgccgccagg atgagcgccg
     9601 cgaccagaag caacttgctc cgcgggtcga gccggaaaaa ccagccctca cccgcctggt
     9661 actggccgaa gtaggcaaaa ttttgttctc cgaaagcacc catagaaatt aggaagtctt
     9721 gccaagatca tttgtccgtg ggatcaattc cacggtgtca atatatccgc gttgcggggg
     9781 agggtcaaga taaagaccct cttttatgca cggcatctcc ttgacactga gggaaaggac
     9841 gctttcttgc gcagggttat gtcctttcag gaactcccgc agaatttccg ccgcaactac
     9901 cgtctgagca tcgccaacgg cctctttttc aatgcggcca acgccttcat caccggaacg
     9961 accatcatcc cgctcttcgt cagccgcctg acagcttcga cgattctgat cggcctggct
    10021 gccagtctgg aaaccgctgg gtggcttttg ccccaggggc tggtggcggc gatgaccttg
    10081 gacaacgtct accagaaacc acagtacatt cgtgtggcca tcgtgcgggt cattgtcttc
    10141 gccgccctgg tgacctccgt ttttcttttt ggccgcagtt acccaacact ctgtcttgtg
    10201 tcttttttcc ttttcttttc catctacgcc atgggcggcg gctccgccgg ggtggctttt
    10261 atggatatcg tggccaaaac catcccccac cacaaacgcg gcagtctctg gggctggcgc
    10321 atggccttgg gcggtagtct ggcggcagta atgggcctcg tgatcaaaaa acttttagcc
    10381 gccttctcct tccctgtgaa cttcggcctg gtcttcgcct gcgcctgggt cttgatcgtc
    10441 gccgggctta cttccttttc ttttgtgatc gaaccgcccg gagtaaaccg gctcccgcgc
    10501 ctttccattc ggcagggatt tcagaagttt ttggaaattt tccggcggga tcagaacttc
    10561 aaaatgctct actacatccg cattgccgcc gggtcgtaca tcatcgccgc gccgttttac
    10621 atcgtctacg gcatcaatca cctgggttac ccgccggagg tggccgggct atttttgacc
    10681 gcgcagatga tcggcctggt cattaccaac cccctctggg ctaaaatcag caacacccgg
    10741 agcaatcgtt tagtgctctt ctggtcggcg gtcttctcca cgctgccgcc ggtggtcgtg
    10801 ttgctttcga atctgacgcc cctgcccgcg gcgctctacg cggccatttt ctttttaatg
    10861 ggcgtggccg aagcgggact gacgatcggc tacttgaatt acgttttgga aatcgccccg
    10921 gagcccttgc gccccctcta caccggtttc ctgcacacca tcatcgcacc gaccgtggtt
    10981 ttctccgccc tcggcggcct ggtcattcaa ctgacctcgt tcaccgctct tttcataatt
    11041 gcgctcgtaa tcggcctcgg cgcagtggtg ttagcaaaaa aattagcgtt cgcatgattg
    11101 taggggcgag atcccctcgc cccttctttt taacaggatg taatgtccct ccccttcgag
    11161 gggagggtag ggaggggtca atgttccttc tctcgcgtag gcggcacttc agtgccgccc
    11221 agcattccct ctcctgtagg agagggcggg gtgaggtcaa tgcactcgca cctgcctcac
    11281 gtcactggac aggaggtact cctcttccgc ctgcggtaat acacataccc cagcatcccc
    11341 gccagaagca ccccgaagag cgccagcccc cactgcgtca aggtcggcac caccgttgcc
    11401 cccgtgctcc cggccgccat cgagactacc gccgacaacg acaccacccg cgccgcagca
    11461 ccaacaccca gcgtgccgaa gaacgttatc ctgcacgttc ccgtgtcccc gatggacgcc
    11521 gtcggtgcct gacagactct caaaaacccg ttgccggaat cggtcgcgca gctgaaaggc
    11581 tcgaaggtct tcccaaccaa attcgtagcc ccgactgccg tactgcacga caagtccgga
    11641 tcgccgctat gctgaacttt gacgctgtct atggtcaacc gcagtgacgt tgtatcgcac
    11701 aaggtcccgc ccaccgtcac gcaggactcc ttgcccttga acagtgtgac ctgagtcgtg
    11761 acgagagtca ttgcgttcgg tacgctgccg attaactcca ccccgcgaac gaaggccggc
    11821 acattcgcct gcgtcggggg atgggtgaac ttcaggaact ggttgggcga tgtccggttg
    11881 gagtccagcg agtaggtgcc ggggttcgtg ttgtttgtta cgaaaatttt gtacgctatt
    11941 cgcctggggg caggcggggc actaccccca cctgagtcta tcgacaggac aaacatagag
    12001 tccgggcttg cgccgttcac tctcgtcagg ctgggggtca ttcccaacgg gaagatagcc
    12061 ggagtatatt gtatggagtc aaccctgccg aacgccccgc ctgtttctga ccggataacg
    12121 aggggcagat ttatgctgtt aatggccgaa tcgttgtgga tcttaacctc gatgacgccc
    12181 tggcttcccg aagggaacgt cttggatacc acaatgaccg aatcgacaaa cggcgcggca
    12241 agtgcctcct cagccgcccc ggcaatcagg agcagataac cggcaatcgc gaaaagagta
    12301 atacgtcgca tggcttacct tcctttcatc gcaaatttcc gccgcctgaa atagacatac
    12361 cccagcatcc ccgccaaaag caccccaaag cccgccagcc cccactgcgt taaagtcggc
    12421 accaccgtcg ctccggtgct tccagccgcc atcgagactg ccgccgaaaa cgccaccacc
    12481 cgcgccggtg cgcccgcctc acccggtaac gtgccgaaga acgttatcct gcacgttccc
    12541 gtgtccccga tggacgccgt cggtgcctga cagactctca aaaacccgtt gccggaatcg
    12601 gtcgcacagg agaaggcact cagggtcttc cccaccaaat tcgtcgctcc gacggctgtc
    12661 atacacaaaa ggtcggggtc gccgctgtgc tggactctga cgctgtcgag ggtcaaggtc
    12721 tgcgccgtag tgtcacaaat aaccccaccc acattcacgc aggcgtccct tcctttgaac
    12781 agagtcacct gagttgccgc cagattcagc gcgtcgccga ttatctccac cccgcggatg
    12841 aagaccggta tactcacctg ggtcggcgga tgggtaactc tcaggaattg ggtggaagaa
    12901 aaccggttgg agtccaggga ataagtaccg acaaggctgt tgttggtgac aaaaacccga
    12961 tacccacccc gacgtgggcc gggggggatg cttccgccgc cagagtcaat cgagagaatg
    13021 tacagtgagt ccgggctggc accatctatc ctaaagggac cagcagcccc aaatatattg
    13081 agtgcaggta agcgcttgat agaatctact ctcccaaacg ctccacccgt ttccgacttg
    13141 atgaccaggg gtagagcaat accgttgatg gccgaatcgt tgtggatcaa aatatcaatg
    13201 gtgcctgcgc tcccgggggt gaagaccctg gaaaccacaa tcaccgaatc gacgaacggc
    13261 ccggcagcaa cctcctcagc cgccccggca atcaggagca gattaccggc aatcgcgaaa
    13321 atagtaagac ttcgcatgga ttaccttcct ttcatcgcaa atttccgccg cctgaagtag
    13381 acatacccca gcgtccccgc taacagcacc ccaaagcccg ccagccccca ctgcgttaaa
    13441 gtcggcacca ccgttgcccc cgtgctcccg gaagccatcg agattgccgc cg
//
LOCUS       DBNGZD010000311        16106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_329101, whole genome shotgun sequence.
ACCESSION   DBNGZD010000311 DBNGZD010000000
VERSION     DBNGZD010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 16106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16106
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_329101"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..3642
                     /locus_tag="AC16GM_08295"
     CDS             148..3642
                     /locus_tag="AC16GM_08295"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HIR2923712.1"
                     /translation="MADVYCLAINSSGQIFAGRANAGVFRSTDNGGSWTQTGLTVSTV
                     WSLAINSSGVIFAGTYGEGVFRSTDNGGSWTQTGLTNTDVRSLAINSNGQIFTGDFNG
                     GVFRSTNNGVSWTQINTGLTNTDVFSLAINSSGQIFAGTYGGGVFRSTDNGGSWTQTG
                     LMNTFVFSLAINSSGQIFAGTYSGGVFRSTDNGSSWTQINTGLTNTDVRSFAINLSGV
                     IFAGTYRTGVFRSTDNGGSWTQTGLTNTKVVSLAINSNGQIFAGTSLGAVFPYLPELP
                     LPPTGTNLGVFRSTDNGGSWSQTGLTNTDVFSLAINSSGVIFAGTQDGVFRSTDNGNS
                     WTQTGLMNSQIISLAINSSGQIFAGEVTGPGRDSAVFRSTDNGSSWTKTGLEDESVYS
                     LAINSSGHIFAGTYAGGVFRSTDNGGSWTQINTGLTNGFVFSLAINSSGVIFNGTYGG
                     GVFRSTDNGGSWTQINSGLTNTQVMSLAINSSGVIFAGTVGGGVFRSSGILQQPIATA
                     VLDAGLGDKLIVKWRSPLDETGIAEYQIGYGTTPGVYVGLSTHLATGQPTYQDIVRGL
                     QPNTLYYVAVRSRNGNTGQVSSWTIATTAVAPTLPVVLVHGWGKGNPGIWGDNNRGYV
                     KWLHDAGFAHVWVCDKINSCGYQGESQFDLNAKALGEFIEDSITSYSNSYSVQPNEYV
                     AINVIAHSMGGLISRRYISDPNPNWVQRRVGEIFMFGTPNAGTEAGTKNCTNRFVCCI
                     GPAAYEMSPENIKWFNQNFGNRPGTAYYVVAGTGGMRSCDPKIPYLNCYGIVLGDILD
                     CPNDGVVSVASVLGGPDRVFRATDKTIRFLCHSDYYDSQDLFNTFVEPKLKSQTFLSV
                     ASVESSVDSHMANDSGQAQIFRKFCSEFITGALIVDSFWVESSHSFTVAVVVEDSAAT
                     FTLKTPSDTLIDSSAGVLDTNVYFGHLFNIRTFSITNPEPGLWKAYLQTSVGGLDSNV
                     CFIAGVGSDVLMTTDLLPRDVLPGDSIVVLACLTESGLPLTGVSISVLPVAFRADTLP
                     VFSLFDDGAHRDSSAGDGVYGAVFTQTGDSGLVTFNVVATGLAPSVGSFRREASLVAS
                     ISLGAPCIILGYRGDVNNDATTDIVDIVTLINDVVFSTGLPNPLAGDTDCDGVRDIVD
                     IILLIQYVVFGTPSPCCL"
     gene            3839..6157
                     /locus_tag="AC16GM_08300"
     CDS             3839..6157
                     /locus_tag="AC16GM_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HIR2923713.1"
                     /translation="MTYTNRLIKETSPYLLQHAHNPVDWYAWGPEALEKAKRENKPIF
                     LSIGYSACHWCHVMEEESFSSPEIAAVMNRHFVSIKVDREERPDLDDIYMNAVQMMTG
                     SGGWPMSVFLTPDLKPFFGGTYFPPDDRYGRPGFKSVLQQIADSWQNRRSDIDQFANR
                     VSLALEQATAAKPESKEVDPTVIAKAVESASGGFDSTYGGFGSAPKFPPSLTLELLLT
                     QDQQKPDEKVREMVETTLDFMARGGVYDQVGFGFHRYSTDRVWLVPHFEKMLYDNALL
                     SRDYVLAYQVFKKPFYRRIAEQIYEFISRETTDPEGGFYSTIDADAEGEEGKFYVWTK
                     AEVEQVLGESDGKLFCDVYDISTTGNFEGKNIPHLARTVEDWAKTLNRNPQELQSHLD
                     RLREKLLEVRGKRVRPHLDDKILTDWNGLMISSYARAFTVLGNSEYRTKAEKTANFIL
                     SKMTSGDTLFHTYRNGERKLPSFLDDYAFFIEALLDLSDATQDAKWFSEARRLTDAMI
                     ARFWDQDNGGFFFTASDHNAPLVRTKSAQDNVTPSGNSVAILSLLRLAEKTSDQTYLR
                     LAEKGIGAFGDLISKMPTGFAYMLLAVDRLNSPALASLKTKTTTPLEPSDLVTLTGYL
                     SVDKVQPGRPFRLAVDIAIKKGWHINSHRPNLDYLIPTKIGFDGLPKVKVRKVEYPSG
                     ADVKLGFADEALSVYQDTVRLVAEMELADGVKTRTGNIAVTLNYQACDDEKCLRPTKV
                     TAEIPVSVASKGEAVQSLHPEIFGGKRKAGSP"
     gene            6317..6835
                     /locus_tag="AC16GM_08305"
     CDS             6317..6835
                     /locus_tag="AC16GM_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR2923714.1"
                     /translation="MIDKNAHLPETDDGTILFNVPFPKDLKELDKMAPIVIKSPYQID
                     YLHSYGQDSPFFAGLANRKLLGTKCLRCRYKYATPRGHCMYCGEKTEWFELPQEGKIH
                     TWTTCYFGSEVFLPECPFNLALLEFEGVDTLLLVRVIGAETKDLKVGMKLKARFRRNS
                     KFLPTDVYFVPA"
     gene            7133..7852
                     /locus_tag="AC16GM_08310"
     CDS             7133..7852
                     /locus_tag="AC16GM_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR2923715.1"
                     /translation="MNKVVQNATAALSHAKLHDGMTIMVGGFGLCGIPENLIAALRDS
                     GVKELTVISNNAGVDDFGLGLLLQTRQIKKMVSTYVGENSIFERQFLDGVLEVEFVPQ
                     GTFAERIRAGGAGIPAFYTPTGVGTVIAEGKETRLFEGREYVLERGLRAELSLIKACK
                     GDPAGNLIYRKTARNFNPMMATAADFTIAEVEHVVEIDQLDPDSIHTQGIYVDLLFQG
                     VQYEKRIERRTTRPKAAVTTR"
     gene            7849..8556
                     /locus_tag="AC16GM_08315"
     CDS             7849..8556
                     /locus_tag="AC16GM_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HIR2923716.1"
                     /translation="MKNPTVSPAAKEPQKSQGADPRELIIRRAAKELRDGYYVNLGIG
                     MPTLVANYIPPGMDIVLQSENGLLGIGPFHYAGEEDADLINAGKETVTALPAASYFSS
                     ADSFAMIRGGHIDLSILGAMEVDQEGNLANWVIPGKMVKGMGGAMDLVAGAKRVVVTM
                     LHTAKDGSSKVLKQCKLPLTGRKVVHRIISDLAVMDVTPQGLLLLEVAPGVTVEQVKK
                     STEADLAVSNRVREMEG"
     gene            8653..9561
                     /locus_tag="AC16GM_08320"
     CDS             8653..9561
                     /locus_tag="AC16GM_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923717.1"
                     /translation="MRFLLVLFITVGGHSRFAVVAFAQRDLPLCAVKQADPPQAGWYV
                     EPMVFVHRESESPVVYRYPQPGEAIWKYAFPGGARFDLFRWGVKVGEFVLKRFQSSLE
                     DSCQGARAWGQPAWGTTIESPDELIAVRTGEYPEAQPNWSALDSVAASGEAIPQTRQL
                     LQKQGVPAGVTLKCEVSQLQTFQINHTPFFAVTLAYRPPAGDSTSAEQISRGVGALVI
                     TKLLRRSKKESLYQPVLTRVAQWDENEPPVTWDLVGLFDADQDGMPELATRRKENGTY
                     TYEISVMESGRYRSVYESDRRGCAQK"
     gene            9592..10776
                     /locus_tag="AC16GM_08325"
     CDS             9592..10776
                     /locus_tag="AC16GM_08325"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR2923718.1"
                     /translation="MNGEPVILSACRTAIGNFLGGLATVSAPRLGAVAVREVVKRAGI
                     EPNEVDEVMLGHVVQAGAGQAPARQAAIHGGIPSSVPCMTVNKVCGSGLKTVMCAAQS
                     IRAGDNQVVLAGGMESMSNTPHLLHGAKLGFRFGDQELKDAMILDGLWDSFNNFHMGS
                     AAELTAKKSNISREEQDEFAYNSHMKAAAAIRNGKFKAEIVTVEVPQKKGDPAKFELD
                     ECVRPDTSIEKLSKLRPAFEKNGTVTAGNAPGLNDGASTVMVASAEFAKKKGLKPLAK
                     IVGYAAAGTDPELLFYAPIYAVRKLMKQIGVDINHWDLIEVNEAFAAQVLADGKELGW
                     DWKKVNVNGGAVALGHPIGASGARILTTLLYALQDRGLKKGLATLCLGGGNALALAVE
                     MI"
     gene            10813..11199
                     /locus_tag="AC16GM_08330"
     CDS             10813..11199
                     /locus_tag="AC16GM_08330"
                     /inference="COORDINATES: protein motif:HMM:NF017451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2923719.1"
                     /translation="MPETKRIGSVEDLEVYRKLCSLHLDVHNFSLKFPKFEMYELGSQ
                     LRRSSNAVPANLAEGWNNKHTNIYLEGINRAFGELRETLHHLRMASQKGYLQDTVHRN
                     LASRYEECGRMLRGLERALVRQRNGT"
     gene            11290..12429
                     /locus_tag="AC16GM_08335"
     CDS             11290..12429
                     /locus_tag="AC16GM_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2923720.1"
                     /translation="MDFELTENQILLRDTAREFAQKRVAPLAQEMDEKEAIPPDLLKE
                     TADLGYFGLLIAEQYGGLGVDMLSYVLAMEEIARASAGLMITISVHNSLVAQGLVHFG
                     SEELKQKYLPKLASGELVGAYALTEPGSGTDAAALITSAVRQNGHYLVNGTKSWVTNA
                     GFAGLYSVFVSTNPALKSKGISCLIVERDLLGLTLGAKEKKMGIKCSDTREISFQDAR
                     VPAANLLGVEHDGYKIALALLDFGRLGVGAQSIGIAQAALDEAVKYSKERQQFGQPIA
                     NFQGIQFKLADMATQIEAARLLVYQAAALEDKPGRWSKEIAMAKLFASEVANYVANEA
                     VQIHGGYGYIKEYAVERYFRDARVTEIYEGTSEVQRMVISRAILT"
     gene            12433..13566
                     /locus_tag="AC16GM_08340"
     CDS             12433..13566
                     /locus_tag="AC16GM_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2923721.1"
                     /translation="MHLEKIYQELRDRIRTFADRELAPHAKDVDEKGEVRRENVAALA
                     REGLLGMLIPKEYGGFAEGTLAYVIALEEVSRACGSTGIIMAAHNSLCCFPIYRFGTE
                     AQRHRYLPDLAAGRVLGAFGLTEPQAGSDAGNTKTTAVLKDGHFIINGQKCWITNASF
                     AGTFVITARTSPGTGADGISAFIVLRTTPGFTSGKKENKMGLRGSDTAFLHFTDMPVP
                     QENLLGPLGVGFKQFMQTLDGGRIGIAAMALGIAQGALDCALAYAGERHQFGQPIGSN
                     QAIQWKLADMTTQLAAARLLTYRAARLKDKGEKTTRESSMAKLFASEAGRYVCYQAIQ
                     ILGAAGYTNRYPVERMYRDVKLCEIGEGTSEIQRLVIAREVLK"
     gene            13698..14714
                     /locus_tag="AC16GM_08345"
     CDS             13698..14714
                     /locus_tag="AC16GM_08345"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR2923722.1"
                     /translation="MLFEEVKKIWMDGKLVDRDQASVSILAHVIHYGSSIFEGIRAYN
                     TPDGPAVFRLPEHLERLYNSCRIKRMEIPYNRKQLLDATLEIIRLHDVRHWTSPYIRP
                     VVYRGLRHLGVDPTGCPVNVAIAVLDMGHYLGEDAKRNGVRVCVSSWNRIAPNTLPAM
                     AKASANYMNSQLIKMEANAILKPDASKKEKVEGISLDVSGHVSEGTGENIFLVRDGAL
                     VTPSFASSILPGVTRNTVIALAQEMGIKVIEEQVPREALYIANEVFFTGTAVEIVAVR
                     SIDGIEVGDPALSPEERWPVTTKIRQRFFDIVTGKEPDRRGWLTYVDKKVKPAVKEKE
                     FVTT"
     gene            14769..15215
                     /gene="rpiB"
                     /locus_tag="AC16GM_08350"
     CDS             14769..15215
                     /gene="rpiB"
                     /locus_tag="AC16GM_08350"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR2923723.1"
                     /translation="MKIALGADHRGFALKENLRAQLQNLGHETIDVGTESEESVDYPD
                     FAMRVGRAVASGQVDQGIAVCWSGNGMMIAANKVKGVRAALALNPEMAQLAREHNDAN
                     ILTLAAKYVSAAEAMKIVEAWLKTSFAGGRHARRVEKIKEAERVSG"
     gene            15229..>16106
                     /gene="glyA"
                     /locus_tag="AC16GM_08355"
     CDS             15229..>16106
                     /gene="glyA"
                     /locus_tag="AC16GM_08355"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR2923724.1"
                     /translation="MAETLSLTDRARPLAAVDPEIFEAIQNETKRQSGQLELIASENF
                     VSRAVLEALGNVMTNKYAEGYPGRRYYGGCEFVDVAENLARDRAKALFGCEHVNVQPH
                     SGSQANMGAYLSVLDIGDTIMGLNLSHGGHLTHGHPINFSGRFFKVVAYGVSKETETI
                     DYDEVRRLAREHKPKMIVVGASAYSRIFHFPKFREAADEIGAFLMVDIAHIAGLIVAG
                     LHPSPVPYADFVTTTTHKTLRGPRGGMIMCRAKYAKDLDRMIMPGIQGGPLMHVIAAK
                     AVAFKEALAPEFKVYQV"
BASE COUNT         3631 a         4385 c         4576 g         3514 t
ORIGIN      
        1 gacaatggcg gtagctggac acaaaccggc ttgacggttt ccactgtctg gtctcttgcc
       61 atcaattcga gcggggcgat ctttgccggg actcgcggcg gtgtctttcg ctccactgac
      121 aatggcaata gctggacaca aaccggcttg gcggatgtct actgtcttgc gatcaattcg
      181 agcgggcaga tctttgccgg gagggccaat gcaggtgtct ttcgctccac tgacaatggc
      241 ggtagctgga cacaaaccgg cttgacggtt tccactgtct ggtctcttgc catcaattcg
      301 agcggggtga tctttgccgg aacttacggt gagggtgtct ttcgctcgac tgacaatggc
      361 ggtagctgga cacaaaccgg cttgacgaat acggatgtcc ggtctcttgc catcaattcg
      421 aacgggcaga tctttacagg ggatttcaat ggcggggtct ttcgttctac taacaatggc
      481 gttagctgga cgcaaattaa caccggcttg acgaataccg acgtcttttc tcttgcgatc
      541 aactcgagcg ggcagatctt tgccgggact tacggtgggg gtgtctttcg ctccactgac
      601 aatggcggta gctggacaca aaccggcttg atgaatacct tcgttttttc tcttgcgatc
      661 aactcgagcg ggcagatctt tgccgggact tacagtgggg gtgtctttcg ctccactgac
      721 aatggcagta gctggacaca aattaacacc ggcttgacga ataccgatgt ccggtctttt
      781 gccattaatt tgagcggggt gatctttgcc gggacttatc gtaccggcgt ttttcgctcg
      841 actgacaatg gtggtagctg gacacaaacc ggcttaacga acacgaaggt cgtttctctt
      901 gcgatcaatt cgaacgggca gatttttgcc gggactagtc tcggcgctgt ctttccctac
      961 ctccctgaat tacccttacc gcctactggg actaatctcg gtgtctttcg ctccactgac
     1021 aatggcggta gctggtcaca aactggcttg acgaatacgg atgtcttttc tcttgccatt
     1081 aattcgagcg gtgtgatctt tgctggcact caagacggtg tttttcgctc gactgataat
     1141 ggcaatagtt ggacacaaac cgggttgatg aattctcaaa tcatatctct tgccattaat
     1201 tcgagcgggc agatctttgc cggggaagta accggacctg gcagagatag cgccgttttt
     1261 cgctccactg acaatggcag tagttggaca aagaccggat tggaggatga atctgtctat
     1321 tctcttgcca ttaattcgag cggccacatc tttgccggga cttacgctgg cggtgtcttt
     1381 cgctcgactg acaatggcgg tagctggaca caaattaaca ccggcttgac gaacggcttt
     1441 gttttttctc ttgccatcaa ttcgagcggg gtgatcttta atgggactta cggtggcggt
     1501 gtctttcgct ctactgacaa tggcggtagc tggacgcaaa tcaacagcgg gttgacaaac
     1561 acccaagtca tgtcccttgc catcaattcg agcggagtga tttttgctgg gaccgtcggt
     1621 ggcggtgttt ttcgttcgtc aggtattctt caacagccaa ttgcaacggc tgtgcttgat
     1681 gcgggtttgg gtgataaact aatagtaaag tggcgttctc cactggatga aacaggcatc
     1741 gctgagtacc agatcggtta cgggactacc ccaggtgtct atgtaggtct gtcaacccac
     1801 cttgccacag gccagccgac ttatcaggac attgttaggg gtttacagcc gaatactctc
     1861 tactatgttg cagttcgttc ccgtaacggt aacacggggc aagtgagtag ttggactata
     1921 gctaccacag ccgtagcccc cactcttccg gtggtgctgg tgcacggctg ggggaaaggt
     1981 aacccgggca tttggggaga caataatcga ggatatgtga aatggcttca cgatgccggt
     2041 tttgcgcatg tttgggtttg cgacaagatt aattcttgtg gctatcaggg cgagtcgcaa
     2101 tttgatctaa atgcaaaggc gctcggcgaa tttattgagg attctattac atcatattca
     2161 aacagttata gtgttcagcc aaacgaatat gtggcgataa acgtaattgc gcatagtatg
     2221 ggggggttaa tttcgcgcag atatattagc gaccccaacc ccaattgggt gcaaagacgt
     2281 gtgggtgaaa tcttcatgtt tggcactccc aacgctggca cagaagctgg cacaaaaaat
     2341 tgcactaata ggtttgtatg ttgtattggt ccagctgctt acgaaatgtc gccagaaaat
     2401 attaagtggt ttaaccaaaa ctttggcaac agaccgggga cagcgtacta cgttgttgca
     2461 ggcactggag ggatgagatc atgtgatccg aagattccct atcttaattg ctacggtatc
     2521 gtcctcggag atatccttga ctgtcctaat gatggggtgg tttctgttgc aagtgtatta
     2581 ggtgggcctg acagggtttt tagggcgaca gataaaacca ttcgtttctt gtgccacagt
     2641 gactactatg atagtcaaga ccttttcaat acgtttgtcg aacctaaatt aaaaagccaa
     2701 actttcttgt cggttgcctc tgttgaaagc tcagtagatt ctcatatggc gaatgacagt
     2761 ggtcaggccc agatttttag aaaattctgc agtgaattta tcactggagc attaattgtt
     2821 gattctttct gggtggaaag ttcccatagt ttcacagtgg cagttgtagt ggaagattca
     2881 gccgcaactt tcacattgaa aacaccgtcg gacactttga tagattcctc tgctggggtt
     2941 ttagatacta acgtatattt tggtcacctt tttaatatac ggacgttttc aatcacgaat
     3001 cccgaaccgg ggctgtggaa agcatacctg caaaccagtg tcggtggact agattccaat
     3061 gtttgcttca tcgcgggtgt tggcagtgat gttctgatga ccaccgatct tttacctcgc
     3121 gatgttttgc caggggattc tattgttgtt cttgcctgcc tgactgaatc cggtttgccg
     3181 ctcaccggag tcagtataag tgtccttccc gtcgctttca gagccgacac cctccccgtt
     3241 ttttcgctat ttgatgacgg tgcccatagg gatagctccg ctggtgatgg cgtgtacggc
     3301 gctgtattta cccagacggg agacagcggc cttgttacct ttaacgtggt ggctacaggt
     3361 ttggcaccat ctgtcggatc gtttcgccgc gaagcaagtt tagtagcgtc tatttcgctt
     3421 ggcgctccct gcataatcct tgggtatcgc ggcgatgtaa ataacgacgc cacaaccgac
     3481 attgtggaca ttgttactct gatcaacgac gtggttttct caaccggatt acctaatcct
     3541 ctcgccgggg acaccgactg cgatggagtg cgggatatag tggatattat cttgctcatt
     3601 cagtacgtag tattcggcac tccatctcct tgctgtttgt aagagaatct gacctaaccc
     3661 aaccctcacc cacaggagag ggagacgtcg tatcaataaa aaaggactac ttatctcttt
     3721 caagaagcgc ctcctctggt tatcatctct tccggcagga gcgctcgttc tcgtgggcgg
     3781 gtggctccta ctccgtagca ccggtgcact ctgctcatcg cctgataaga agtcaaacat
     3841 gacctataca aatcgcttaa tcaaagaaac cagtccctat ttactccagc acgcgcacaa
     3901 cccggtggac tggtacgcct gggggccgga ggcgttggag aaggccaaaa gggagaacaa
     3961 gccgattttc ctttccatcg gctactccgc ctgccactgg tgccacgtga tggaggagga
     4021 gtcgttttcc tcgccggaga ttgccgcggt gatgaaccgg cacttcgtct ccatcaaggt
     4081 ggatcgggag gagcggcccg atctggacga catctacatg aacgccgtcc agatgatgac
     4141 cggctcgggt gggtggccga tgtcggtttt cctcacgccc gacctcaagc ccttcttcgg
     4201 cggcacctac ttcccgccgg atgatcggta cgggaggccg gggttcaaaa gcgttttaca
     4261 gcaaattgcc gattcctggc agaatcgccg gtcggatatc gaccagttcg ccaaccgggt
     4321 cagtctggca ctcgaacagg cgacggcggc caagccagag tccaaagaag tcgacccgac
     4381 cgtcattgcg aaagcggtcg agtccgcctc aggcggattc gattcgactt acggtgggtt
     4441 tggctcggcg ccgaaattcc cacccagtct gacgctggag ttgctcttaa cacaggatca
     4501 acagaagccc gatgaaaaag ttcgcgagat ggtggagacg acgcttgact ttatggcgcg
     4561 gggcggggtg tacgatcagg ttggctttgg cttccaccgc tactccaccg accgggtctg
     4621 gctcgtgccc cacttcgaga agatgctcta cgacaatgcg ctcctctcgc gggattacgt
     4681 tttggcttat caggtcttca agaaaccgtt ttatcgccgc attgccgaac agatttacga
     4741 atttatctcc cgcgaaacga ccgatcccga gggcgggttt tactccacca tagacgccga
     4801 tgctgagggg gaagaaggga aattctacgt ctggacgaaa gcagaagtcg agcaagtttt
     4861 gggcgaatcc gacggcaaat tgttctgcga cgtctacgac atcagcacca ccgggaactt
     4921 cgaaggcaaa aacatcccgc accttgcgcg gacggttgag gactgggcga aaacgctcaa
     4981 ccgcaatcca caagagttac aaagccacct cgaccgcctg cgggaaaaac ttcttgaagt
     5041 acgcggcaag cgcgtccgcc cacatttgga cgacaaaatc ctcaccgact ggaatgggtt
     5101 gatgatttcc agttacgcac gcgctttcac tgtgcttggc aattcagaat accggacgaa
     5161 ggcggagaaa acggccaact ttattctttc caaaatgacc tccggtgata ctctttttca
     5221 tacttaccgc aatggcgaac gcaaactccc cagctttctg gatgattacg ctttcttcat
     5281 cgaggcgctt ctggacttgt ctgacgccac gcaggatgcg aaatggttta gcgaagctcg
     5341 ccgcctgacc gacgcaatga ttgcgcgctt ttgggaccag gacaatggcg gatttttctt
     5401 taccgcctcc gatcacaacg cgcccttagt ccgcaccaag agcgcgcagg ataacgtcac
     5461 gccctcggga aattcagtgg cgattctttc actcttgcgc ttggccgaaa agaccagcga
     5521 ccagacctac ctccggctgg cggaaaaagg catcggcgct ttcggagatt tgatttctaa
     5581 gatgcctacc ggctttgctt atatgctcct ggccgtcgat agattgaact cccccgcgct
     5641 ggcaagtttg aaaacaaaga cgaccacacc gctcgaaccc tccgatctcg tcacactcac
     5701 cggctatctc tcggtggaca aggttcagcc tggcaggccc ttccgcttgg cggtggacat
     5761 cgcgatcaaa aagggctggc acatcaactc gcaccggccc aatctcgatt acctgatccc
     5821 gacgaaaatt ggcttcgacg gcttacccaa agtgaaagtt cgcaaagtcg aatatccgtc
     5881 cggcgcagac gtaaaacttg gtttcgccga cgaagccctc tcggtctacc aggacacggt
     5941 gcggttagtg gctgaaatgg aattagcgga cggtgtcaaa acccgcacgg gcaacattgc
     6001 ggtgacactc aactaccagg cctgcgatga tgagaagtgt ctgcgtccta cgaaggtaac
     6061 cgctgaaatt cccgtatcag tagccagcaa gggagaggcg gtgcaatcgc tccatccgga
     6121 gatttttggg gggaaaagga aagccggttc gccctgatct tcttgcgcga cgagggcgtc
     6181 ccgcctacgc ggaaaggcgt cctgcccgcg tggggcgacc gcccccggtc gcccctccct
     6241 tcgaaaaccg cttgcaaact ttctattact cctataaatt ccgcaccaaa tttcgcacga
     6301 catacctgga gcggctatga tcgacaaaaa cgcgcacctg cccgaaaccg acgacggcac
     6361 catactcttc aacgtcccct tcccgaaaga cctaaaggaa ctggacaaga tggcgccgat
     6421 tgtcatcaaa tcgccgtacc agatcgacta cctgcattcg tacggccagg attcgccctt
     6481 cttcgccggg ctggcaaatc gaaaacttct gggcaccaag tgtctgcgct gccgctacaa
     6541 gtacgccaca cctcgcggcc actgcatgta ctgcggcgag aagaccgaat ggttcgaact
     6601 gccgcaggaa gggaaaatcc acacctggac cacctgctat ttcggctcgg aagtctttct
     6661 gccggagtgc ccgttcaatt tagctttgct tgagtttgag ggagtcgata cgcttttgct
     6721 ggtgcgggtc atcggcgcag aaaccaaaga tctcaaagtc gggatgaaac tcaaagcgcg
     6781 cttccgccgc aacagcaagt ttctgccgac tgacgtctac ttcgttccgg cgtaaggtca
     6841 cgatatagcg agaacggaaa agacgagaag tcccgaagca aagagtagtc agtcggccag
     6901 actgctcttt ttttctctga tggttaaagg ctcctcaaac ccagcgcggt ggtgccggga
     6961 tctcttcggc gcagctccaa gcaggcgttt ttagcgggct cactatgatt tcttgcgttg
     7021 acctggcgct gggtcgcgtg gagggcagtc ggacaaaaaa gaagaggaca gttgaaactc
     7081 tgagctgaaa tacttatact taaatttgag agtcagaaaa caagaatagc ggatgaataa
     7141 agtcgtccaa aatgctaccg cagccctgag ccatgccaag ctccacgacg gcatgaccat
     7201 catggtcggc gggttcggcc tgtgcggcat cccggagaac ctgatcgccg ccctgcgcga
     7261 ttcgggcgtc aaggagctca ccgtcatttc caacaacgct ggcgtggatg acttcggcct
     7321 cggtcttttg ctccagaccc ggcagatcaa gaaaatggtc tccacctacg tcggtgaaaa
     7381 ttcaattttc gagcgtcagt ttctcgacgg agtcctggaa gtggaatttg taccgcaggg
     7441 aacgtttgcc gagcgaatcc gggcgggcgg ggccggcatt ccggcgttct acacacccac
     7501 cggtgtcggc acggtcattg ccgaagggaa agagactcgc ttgttcgagg gacgggagta
     7561 cgttttagag cgcggcctgc gcgcggaact gtcgctcatc aaggcctgca aaggtgaccc
     7621 ggcgggcaac ctcatctacc gcaaaacggc gcgcaacttt aacccgatga tggcgaccgc
     7681 ggcagatttt accattgccg aagtcgagca cgtagtggag attgaccagt tagacccgga
     7741 cagtattcat acacagggga tatacgttga tttgctcttc cagggtgtgc agtacgaaaa
     7801 gcgtattgag cgccgcacca cgcggcccaa agcggcggtc acaacccgat gaaaaatcca
     7861 accgtatctc cagcagcgaa agaaccacag aaatctcaag gggctgaccc gcgggaattg
     7921 atcatccgcc gcgccgcaaa agagcttcgc gacggctact acgtcaattt aggcatcggc
     7981 atgccgacct tggtcgccaa ctacatccca ccgggaatgg atatcgtgct ccagtcggaa
     8041 aacgggctct tggggatcgg tcctttccac tacgcagggg aagaagacgc ggatttaatt
     8101 aacgccggga aggagacggt caccgcgctg ccggccgctt cctatttctc cagcgccgac
     8161 tctttcgcga tgatccgcgg cgggcatatt gacctctcga ttttaggtgc aatggaagtg
     8221 gatcaggaag gaaacttggc caactgggtg atccccggaa aaatggtcaa gggaatgggc
     8281 ggcgcgatgg atctggtcgc tggcgccaag cgggtggtcg tcaccatgct ccacaccgcc
     8341 aaggacggct cctccaaggt gctcaagcag tgcaaactgc cgctgacggg aaggaaagtg
     8401 gttcaccgca tcatcagcga cctggcggtg atggacgtga ccccgcaggg attgctcctg
     8461 ctggaagtgg caccgggagt gacggtggaa caggtcaaaa agtccactga ggcggatttg
     8521 gccgtcagta accgagtaag agaaatggaa ggatagacag aaaatagcaa tggttctcgt
     8581 cctttacctt tacgggtgtt ataaggaaag ccatacgaat aacagtaaaa acagcgctgc
     8641 aacggattgc gcgtgagatt tcttctcgta cttttcataa cggtcggtgg acattcgcgt
     8701 tttgccgtcg tggcgtttgc ccagcgtgac ctgcccctct gcgcagtcaa acaggcggac
     8761 ccgcctcagg cgggctggta cgtggaacca atggtttttg tgcaccgaga gtcggagagc
     8821 ccggtggttt atcgataccc ccagccgggt gaggctatct ggaagtatgc tttccccgga
     8881 ggagcgcggt tcgacctctt tcgctggggc gtaaaagtgg gagaattcgt gctgaagcga
     8941 tttcagtcct ctctggaaga ttcctgccag ggcgcccgtg cttggggcca gccggcgtgg
     9001 gggacgacta tcgaaagtcc ggacgaactg atcgcggtgc gcaccggaga atatccggaa
     9061 gctcagccga actggagtgc gcttgattca gttgccgcga gcggcgaggc gataccccaa
     9121 acgcggcagc tgctccagaa gcagggcgtt cccgcgggag tgacgttaaa atgcgaagtg
     9181 agccagctcc aaaccttcca aataaaccat acgccgttct ttgccgtcac cttggcctat
     9241 cggcccccgg ccggggactc gacttcagcc gagcagatct ctcggggcgt gggcgccctg
     9301 gtcataacga aacttctgcg ccgttcgaaa aaagaaagcc tgtatcagcc cgtgctcacc
     9361 cgggtcgcgc aatgggacga aaacgaaccc ccggtcacct gggacttggt cggcctcttt
     9421 gatgccgatc aagacggtat gccggagtta gccacacggc ggaaagagaa tggtacgtat
     9481 acttatgaga tttccgttat ggaatcggga cggtatcgca gcgtctatga aagtgatagg
     9541 cggggttgtg cacagaaata agaacgccgg ttttcggagt gaggagggga tatgaacgga
     9601 gagccagtca ttctgtcggc ctgccggacg gcaatcggaa attttttggg cggtctggca
     9661 acggtgtcgg cgccgcggct gggtgcagtc gcggtgcggg aagtggtcaa gcgcgcgggt
     9721 atagagccga atgaagtcga tgaggtcatg ctgggccacg tagtgcaggc gggcgccggg
     9781 caggccccgg cccggcaggc ggcgatccac ggcggcattc cgtcctcggt accctgtatg
     9841 acggtgaaca aagtctgcgg ctcaggctta aaaacggtga tgtgcgccgc acagtcgatt
     9901 cgagcggggg ataatcaggt cgtcctggcc ggcgggatgg agtcgatgtc caacacgccg
     9961 cacctcctgc acggcgccaa gctggggttc cgcttcggcg accaggaatt gaaagacgcg
    10021 atgattttag acgggttgtg ggactccttc aacaatttcc acatgggcag cgcggcggaa
    10081 ctgaccgcca aaaagtcgaa catttcccgg gaggagcagg acgaattcgc ctacaacagc
    10141 catatgaaag cggccgcggc gatccgcaac ggcaagttca aagccgaaat cgttaccgtg
    10201 gaagtgccgc agaagaaagg cgacccggcg aagttcgagc tggacgaatg cgtccgcccc
    10261 gacacgtcga tcgagaaatt gtcgaaattg cgtccggcgt tcgagaagaa cggcaccgta
    10321 accgccggca atgcgccggg cttgaacgac ggggcttcca cggtcatggt tgcctccgca
    10381 gagttcgcca agaaaaaagg gctgaagccc ctggccaaga tcgtgggcta cgccgccgcc
    10441 ggaaccgatc cggagctgct cttctacgcc ccgatttacg ccgtgcgcaa gttgatgaag
    10501 caaatagggg tggatatcaa ccactgggac ttaatcgaag tgaacgaggc cttcgccgcg
    10561 caggtcttag ctgatgggaa agagctgggc tgggactgga agaaggtaaa tgtcaacggc
    10621 ggcgcagtgg ctttgggcca ccccatcggc gcctccggcg cccgcattct caccacgctc
    10681 ctctacgctc tgcaggaccg ggggctcaag aaagggctgg cgacgctctg tctgggcggc
    10741 gggaacgcct tggcgctggc agtggaaatg atatgagacg gtggtgggtg atgggtggta
    10801 ggtggtaggg gaatgccgga aacaaagcgg atagggagcg tcgaggatct tgaggtctac
    10861 cgaaaactct gttccctgca cttagacgta cacaactttt ccttaaagtt tccgaaattt
    10921 gaaatgtatg agttggggag tcagctccgg aggtcatcga atgcagttcc agcaaactta
    10981 gctgagggat ggaacaataa gcacacgaat atctatttag aagggatcaa ccgggctttc
    11041 ggcgaactgc gtgaaacgct ccatcatctc cgtatggctt ctcaaaaagg gtatctccag
    11101 gatacggtcc accgaaatct ggcctcaaga tatgaagaat gcggccgcat gctccgcggc
    11161 cttgagcgtg ccctcgtacg gcagcgcaat ggtacatagc cttgttttgc ctatcaccca
    11221 ccacctatca cctaccacct tgtacctgca accgagcaat tcgtttccat ttgtgccaca
    11281 ggagtgacgt tggattttga gctgaccgaa aaccagatcc tcttgcgcga cacggcgcgg
    11341 gagttcgccc agaaacgggt ggctcccctc gcgcaggaaa tggacgagaa ggaagccatc
    11401 ccgcctgatc tactcaaaga aaccgctgat ttaggctact ttggtctttt gatagctgag
    11461 cagtatggcg gtttgggcgt cgatatgctg agttacgtcc tggctatgga agagatcgcc
    11521 cgggcgtccg ccgggctcat gatcaccatc tcggtgcaca actcgctcgt ggcgcagggg
    11581 ctcgtccact ttggttccga ggagttgaag cagaagtacc tgccgaagct cgcctcaggc
    11641 gaacttgtcg gcgcttacgc cctcacggag cccggctcgg gcaccgacgc ggcagctctc
    11701 atcacctcag ccgtgcggca gaacggccac taccttgtca acggcaccaa gagctgggtt
    11761 accaatgccg gtttcgccgg actttactcc gttttcgtct ccaccaatcc cgccttgaaa
    11821 tccaaaggca tttcctgttt gatcgtcgag cgcgacctat tggggcttac gctgggtgcc
    11881 aaagagaaaa agatgggaat caagtgctcc gacacccgcg aaatttcgtt ccaagatgcc
    11941 cgggttccgg cggccaatct cttgggcgtg gagcatgacg gctacaagat tgccctggcg
    12001 cttctggact tcgggcgtct gggcgttgga gcacaatcga ttggtatcgc gcaggccgcc
    12061 ttggatgaag cggtcaaata ctccaaagaa cggcagcagt tcggccagcc gatcgcgaac
    12121 tttcagggta ttcagttcaa attagccgac atggcgaccc agatcgaagc agcccggctt
    12181 ttagtttacc aggcggcggc gctcgaggat aaaccgggac ggtggtcaaa agaaatcgcc
    12241 atggccaaac tttttgcgtc ggaagttgcc aactacgtgg cgaatgaagc ggtgcagatt
    12301 cacggcggct acggttacat caaggaatac gccgtcgaac gctacttccg ggacgcgcgg
    12361 gtgaccgaaa tctacgaagg cacctccgaa gtccagcgca tggtcatctc ccgcgccata
    12421 ttgacctaac cgatgcactt ggaaaaaata taccaggagt tgcgcgaccg aatccgtacc
    12481 tttgccgaca gggaactcgc accccacgcc aaagatgtgg atgaaaaagg cgaggtgcgg
    12541 cgagaaaacg tggcggccct ggcgcgggaa ggtctcctgg gaatgctcat cccgaaagaa
    12601 tacggcggct ttgcggaggg caccctcgcc tacgtcattg ccctggagga ggtctcgcgc
    12661 gcctgcggct ccaccggcat catcatggcg gcgcacaatt cgctctgctg ttttcccatc
    12721 taccgttttg gaactgaggc gcagcgccac aggtatctcc cggacctcgc cgccgggcgc
    12781 gttttaggtg ctttcgggct caccgagccg caagcgggct cggacgccgg caacaccaaa
    12841 accaccgccg tgctcaaaga cggtcacttc atcatcaacg gccagaagtg ctggatcacc
    12901 aacgccagtt tcgccggtac cttcgtcatt accgcccgca ccagtccggg caccggggcc
    12961 gacggcatct cggccttcat cgtcctgcgc accacacccg gttttacttc aggtaaaaag
    13021 gagaacaaga tgggcctgcg cgggtcggac acggcatttt tgcacttcac cgatatgccg
    13081 gtgccgcaag agaatctgct cggtcccctg ggcgttgggt ttaagcagtt catgcagact
    13141 ctggacggcg gccgcatcgg cattgcggcc atggcgttgg gaatcgctca gggggcctta
    13201 gactgtgccc tggcctatgc cggagagcgg caccagttcg gccagcccat cggttccaac
    13261 caggccatcc agtggaagtt agccgatatg acgacgcagt tagccgccgc ccggcttttg
    13321 acctaccggg ccgcccggct caaagataaa ggggagaaga ccacccgcga atcatcgatg
    13381 gcgaaactgt ttgcctccga ggccgggcgt tacgtatgtt atcaggcgat acagattctg
    13441 ggcgccgccg gctacaccaa ccgatatccg gtggaacgga tgtaccgcga cgttaaactg
    13501 tgcgagatcg gcgaaggtac ctcggagatt caacgactgg tcattgccag agaggtgttg
    13561 aaatagtgtc gaggtgtcgc ggttcgacag gctcaccgtc caaagcaaag tcgagggaaa
    13621 gtgtcgaggt gtcgaggttg aaagagtgat aagcgcccat cttgagaata attacggcct
    13681 ctgtcaggga ggagaccatg ctgtttgaag aggtaaagaa aatctggatg gatggaaaat
    13741 tggttgatcg cgaccaagcc tcggtttcga tcctcgctca cgtcatccac tacggctcct
    13801 cgatttttga aggcatccgc gcctacaaca ctcccgacgg cccggccgtt ttccgcctgc
    13861 cggagcattt ggagcggctc tacaattcct gccgcatcaa acggatggaa attccgtata
    13921 accgcaagca actgttggac gcgaccctgg aaataatccg cctgcacgat gtccgccact
    13981 ggacgtcccc ttacatccgg ccggtggtct accgtggcct gcggcattta ggcgtcgacc
    14041 ccaccggctg ccccgtcaat gtcgccatcg cggtcctgga catgggccac tacctgggtg
    14101 aggacgccaa gcggaacggc gtacgggttt gcgtctcttc ctggaaccgg attgcgccga
    14161 acacactgcc ggccatggcc aaggcctcgg ccaattacat gaacagccag ttgattaaaa
    14221 tggaagccaa tgccattctg aagccggatg cgtccaaaaa agaaaaagtc gaaggcatct
    14281 cgctggacgt ctccggacac gtctccgaag gcacgggcga aaacatcttc ttagtgcgcg
    14341 acggagcttt agtcacgccg tccttcgcgt catcgattct gccgggagtg acccggaaca
    14401 ccgtcattgc cttagcgcaa gagatgggga ttaaggtgat cgaagagcag gtaccccggg
    14461 aagccctcta catagccaat gaagtcttct tcaccggcac cgccgtggag atcgtcgcgg
    14521 tccgctccat cgacggcatc gaggtcggcg accccgcgct ctccccggaa gaacggtggc
    14581 cggtaaccac caaaatccgg cagcgctttt ttgacatcgt caccggcaaa gagcccgacc
    14641 gccgcggctg gctgacttac gtcgataaga aagtgaagcc ggcggtcaaa gagaaagagt
    14701 ttgtcaccac gtaattcttt tcaccgaatg gtacggctcg gcctgcacgc tggaggaaaa
    14761 tcttttccat gaaaattgcc ttaggcgctg accaccgtgg ttttgcgcta aaagaaaacc
    14821 tgcgggcgca actccagaat ctgggccacg agacaattga tgtcggcacc gagtcggaag
    14881 agtcggtcga ttaccctgac ttcgcgatgc gggtgggacg ggccgttgcc tccggccagg
    14941 tggatcaggg gatcgccgtc tgctggtcgg gaaacgggat gatgatcgcc gcgaacaagg
    15001 tcaaaggggt gcgcgcagct ctggcgctca atcccgaaat ggcgcagtta gcccgcgagc
    15061 acaacgatgc caatatcctc accttggcgg ccaagtacgt ctccgctgct gaggcaatga
    15121 aaatcgtcga ggcctggctg aaaacgtctt ttgccggcgg ccggcatgcc cgccgggtcg
    15181 agaaaattaa agaggccgaa cgtgtctccg gttagcgtcg gctcgtccct ggccgaaacc
    15241 ttaagtctca ccgaccgcgc ccgcccgctt gccgcagtcg atccggaaat tttcgaagcc
    15301 attcaaaacg aaacgaaaag gcagagcggg cagttagaac tgattgcctc ggagaatttc
    15361 gtttcccgcg ccgtgttgga agcgctgggg aacgtcatga ccaacaaata cgccgaaggg
    15421 tatcccgggc ggcgctacta cgggggatgt gagttcgtcg acgtcgccga aaatttagcc
    15481 cgtgaccggg ccaaagcgct cttcggctgt gagcacgtca acgtccagcc ccactccggc
    15541 tcgcaggcca acatgggtgc ctacctctcg gttctggaca ttggcgacac catcatgggg
    15601 ttgaacctgt cccacggcgg ccatctcact cacggccatc cgatcaattt ctccggacgg
    15661 ttcttcaaag tcgtcgctta tggagtttct aaagaaacgg aaaccatcga ttacgatgaa
    15721 gtcaggcggc tcgctcgcga acataagccg aaaatgatcg tcgtcggggc ttcggcctat
    15781 tcgcgtattt tccacttccc aaaattccgc gaggcggcgg atgaaatcgg cgcgttcctt
    15841 atggttgaca ttgcccacat cgcgggctta atcgtcgccg gattgcatcc gtcgccggtg
    15901 ccctacgcgg attttgtcac caccaccacg cacaaaaccc tgcgcgggcc gcggggggga
    15961 atgatcatgt gccgggcgaa atatgccaaa gatctcgacc ggatgattat gcccggcatt
    16021 cagggcgggc ccttgatgca cgtcattgcg gccaaagccg tcgcgttcaa agaagccctc
    16081 gcaccggagt ttaaagtcta ccaagt
//
LOCUS       DBNGZD010000312         6857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_119880, whole genome shotgun sequence.
ACCESSION   DBNGZD010000312 DBNGZD010000000
VERSION     DBNGZD010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_119880"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="AC16GM_08360"
     CDS             complement(<1..1014)
                     /locus_tag="AC16GM_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR2923725.1"
                     /translation="MKSAAPAASPAAQAARPLGTQVFWNSGALLFGSVANKLLRLGAA
                     LFLARYLGATGFGRLALVFAFAEIFRLICDLGLDVVAVRQLARVPSQADRLIPATILL
                     KAGLSVLAILAAGGTALACGYSETLVLLISLATLASAFQSLASGFGALFRARLEVSRF
                     LPVLLAEGAVFFALVVCGTLTRQGLPFFVGVFSVAACLAAVLAFRLARRITSFNWNFD
                     RSLVWDLLRQAWPLTVSLIFGAIYFRIDTILVNALAGDRATGLYSACFKLTETFLLVP
                     SAVAASLLPVLSLLRRENPARMIAAFRKTYQLLLALGIFASGLLAFAAAPLVKAAYGA
                     EFGE"
     gene            complement(1011..2135)
                     /locus_tag="AC16GM_08365"
     CDS             complement(1011..2135)
                     /locus_tag="AC16GM_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HIR2923726.1"
                     /translation="MIPDQFEPEAKNKLTLVRPNPAGGGEGVRWTDLVAAVVARRKLI
                     YAIVGTTTILAVTVSLLLSNRYTARAVILPSGERTNLQKLAGLAGGSLLGLGIDPSAV
                     GIENSSLLFPDVLKSRTVREGVLAATFRFPRGKRTVETTLLEYFGVRSLDRGVKRLKN
                     VTDITMNPKTGVIALEVTLEHPELSAAVANEFIRQLDRYNQETRLSKAKDNEAFIARR
                     LEEVGGELRTAEDDLEKFQAQNRNYYAALAPDLTKELKRLQREVEVKNQVFLTLTQQH
                     ELAKIEAKRDLPIVQVLDYARPPEVKSFPQRSLIVLAAFMLSLAFSIFWALWSAYLGH
                     QLSLEERDRLRQQLQAFSADRRRLWQFVRRPLPKFEVPAS"
     gene            complement(2156..4549)
                     /locus_tag="AC16GM_08370"
     CDS             complement(2156..4549)
                     /locus_tag="AC16GM_08370"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR2923727.1"
                     /translation="MQSQTLAWLATAGLLFFSFAAITSTFAQDLTPEEKARLIVEYEK
                     LKQTTGGAPPDAAKLLETQTYQTPDIYSSIPEPKPDAVKPNAGDKGSIFPEGKMPVPK
                     PAATSSEELKLFGYDIFTAQPSSFAPVEDIAPPVDYTLGPGDNLIVNLWGRVDLELNL
                     TCDREGKVFIPKAGEVIAWGLTVPEFEKRLRSKLSTVYSNFRLTVILGKLRTIKVYVF
                     GEVKRPGGYAVTSLASLFNALYLAGGPNERGSLRQIRLVRQNQLVATVDFYEFLLKGF
                     NRDDLKLLSGDVIYVPVVGPLVKIRGQVKRPAIYELKDAERLTAVIALAGGTLPNAYL
                     ERVMIDRIDAGDRRQLIDLNLRDSTVLAENNLTIHDGDDISVFSKYELRENMVWLTGQ
                     VKHPGTFERTAGMTIRDLLKGGEFLEYEAYRHRADLYRLTHNRVRAIIPVNLDLALAG
                     DSTMNFVLQDRDSLRIYSRQEVEHPKFVFISGEVRQPGKYDLLDSMRLWDLIFLSGNL
                     DKSAYLLRAEVARMNPGKPADVLFVNLADVQADPESPQNLFLKEDDQVFIREIPQYRL
                     QENVKITGEVQFPGEYTLQKENETLYALLKRAGFFTERAFAKGTIITRQEVVAEIRRQ
                     NLPDIVANTQRLVKNDSTGQIERPNLDAFDPAKATRIVVDLEEILRTEGKKGDIVLRR
                     GDQIHVPDLPAGIPVMGAVAANGTINFARGKKLGYYVERAGGFTPNADKKEVRLVKAN
                     GRVYSGGKARGQKVELGDAVVVTTRVQEKKDWLKIFASTASILSGVATTAFVIDRLR"
     gene            4786..5235
                     /locus_tag="AC16GM_08375"
     CDS             4786..5235
                     /locus_tag="AC16GM_08375"
                     /inference="COORDINATES: protein motif:HMM:NF042609.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HIR2923728.1"
                     /translation="MEKLFPLTYEFFSSSPVAEDYILGPPFSNCSAGWSTYPALFSFH
                     IPTYLTPVQNWSHPGGKLRELGAESLSDAELLSIIISPGIKGKSAEKIAEDILAKFQS
                     FKGMTNQPLERFLEIKGLADVKIIRIAACFEIARRIVDQVLAENEKV"
     gene            5327..>6857
                     /locus_tag="AC16GM_08380"
     CDS             5327..>6857
                     /locus_tag="AC16GM_08380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdM family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HIR2923729.1"
                     /translation="MREIIEKEGLDLGLPDVETVGPDRKRPDLVIYETRRSDRILCPV
                     ETKLPYFDVFNESELKEPARKKANQRKAKYFAVTNYKQLVWYSTTKVNENAPEEEQIK
                     GRYFLSEIEDLNEIKEARFSRPTIVALKQFLTDLYEVHTGKKAEPKHPVDEFLVWRLH
                     EKIRVLAKYYSRIIYERYNEDPKFSKELRQWFGDQNWQFAGQPSDFDKAARQTAYLLV
                     NKILFYDLLHAKRPGDLDPLEIPEGLTKGSMLAATLQGYFSQVLRIDYETIYDTDFID
                     SVAFPDSKEVVVEIKALTKLLRRYDLARLGYDIVGRIFERLIPEDERHNLGQYFTRSD
                     VVDLILRFCLQHETDRVLDPACGAGTFLVRAYQHKKMMNQRLEHEKILETLWGNDIAK
                     FPAHLATINLAIKDLGSNENYPRILKKDFFSLIVKQDGFDYENSLKGEIQTLSKVKRE
                     VKHPRYFEAIVGNPPYTRQEEISDISPGDKKYKETLIKRALLDVTERPLAHIGKRAGI
                     HA"
BASE COUNT         1565 a         1944 c         1941 g         1407 t
ORIGIN      
        1 ttcgccgaac tcggcgccgt aggcggcttt gacgagcggt gcggcagcaa acgcgagcag
       61 gccggaagcg aaaattccca gcgccagaag gagctggtag gtcttgcgga aagccgcgat
      121 catccgcgcc ggattttccc ggcgcaggag ggagagaacc ggcaaaagcg acgccgccac
      181 tgcggagggg accagtaaaa aggtctcggt cagcttgaag caggccgagt aaagtccggt
      241 ggcacggtca ccggccaacg cattgaccag gatcgtgtcg atgcggaagt agatggcgcc
      301 gaaaatgagt gaaaccgtca gcggccaggc ctgcctgagc aagtcccaaa cgagagagcg
      361 gtcaaaattc cagttgaagg aagtaatgcg ccgggccaac cggaaagcca agactgccgc
      421 aagacacgcg gcgacgctga agacaccgac gaaaaaaggc aacccctgcc gcgtgagtgt
      481 tccgcagacg accagggcaa aaaaaaccgc tccttcggcc aggagaaccg gcagaaagcg
      541 gctcacttct agccgcgccc gaaagagcgc accgaaaccg gaagccagcg attgaaacgc
      601 ggaggccagg gtcgcgaggc tgatcaaaag cacgagcgtc tccgagtagc cgcaggccag
      661 tgcggtgccg ccggccgcca gaatggccag aaccgagagg ccggctttga gcaggatcgt
      721 ggccggaatc agccggtcgg cctgggacgg cacccgcgct aactgccgca ccgccaccac
      781 gtccagaccc agatcgcaga tcaagcgaaa gatttcggca aaagcgaaga ccagcgccag
      841 ccggccaaat ccggtcgcac cgaggtagcg cgccaaaaag agcgctgccc ccaggcggag
      901 cagcttgttg gcgacactgc cgaaaaggag cgcgccggag ttccagaaaa cctgggtgcc
      961 gagcgggcgg gcggcctgcg cggcggggga ggccgctggc gcagcacttt tcatgaagcg
     1021 ggcacctcga atttcgggag aggacgccgc acgaattgcc agaggcggcg gcggtcggcc
     1081 gagaacgcct gtaactgctg gcgcaaccgg tcgcgctctt caagggagag ctggtgcccg
     1141 aggtaggccg accagagcgc ccagaaaatc gaaaaggcca gagacagcat aaatgcggcc
     1201 agcacaatga gcgagcgctg gggaaaactc ttcacctcgg gcgggcgggc gtagtccaga
     1261 acctggacaa tgggtaagtc gcgcttggct tcgattttgg ccagttcatg ctgctgggtg
     1321 agagttagaa agacctgatt tttcacttct acttcgcgct ggagacgctt taattctttg
     1381 gtcaggtcgg gtgctaaggc cgcatagtag ttccggttct gcgcctgaaa tttttccaaa
     1441 tcgtcttcgg cggtcctcaa ttcacccccc acttcctcca gccgccgggc gatgaaggct
     1501 tcgttatctt tggctttgga gagtcgggtc tcctggttgt agcgatccag ctggcggatg
     1561 aactcgttgg ccaccgcggc ggaaagctcc gggtgttcga gcgtcacctc cagcgcaatc
     1621 accccggtct tgggattcat ggtaatgtcg gtgacatttt tcagccgttt caccccgcgg
     1681 tcgagcgacc gaaccccgaa atactcaagg agcgtcgttt ccaccgtacg ctttccccga
     1741 gggaaacgga aggtggctgc cagcacccct tcccgcaccg tgcgactttt cagaacgtcg
     1801 gggaaaagga gagaggagtt ttcaatgccg actgcggagg ggtcgatccc caggcccaaa
     1861 agactccccc cggcgagccc ggccaatttc tgcaggttgg tccgctcgcc cgaaggcaaa
     1921 atcaccgccc gggcggtata gcgattggaa agcaagagcg acacggtgac ggccagaatg
     1981 gtcgtcgtcc cgacgatggc gtagatcagt ttccgacggg ccaccacggc ggcgaccaga
     2041 tcggtccagc gcaccccctc cccgccgccg gcggggttcg ggcgcactaa agtcaatttg
     2101 tttttggctt ccggctcgaa ctggtcggga atcataggca cttgcgcaag gtcgttcagc
     2161 ggagccggtc gatcacgaac gccgtagtgg cgacgccgga gagaatcgac gccgtgctgg
     2221 cgaaaatctt cagccagtct tttttctcct gtactctcgt cgtgacgacg acggcatctc
     2281 ccaattccac tttctggccg cgcgctttcc cgccgctgta gacccggccg ttcgccttca
     2341 ccaggcgcac ttctttcttg tcggcattgg gggtgaagcc gccggcgcgc tccacgtagt
     2401 agccgagctt cttcccgcgc gcgaagttaa tggtgccgtt ggcggccacc gcgcccatca
     2461 ccggaatgcc ggccggcagg tcggggacgt gaatctggtc tccccgtctg agtacgatgt
     2521 cccccttttt cccttcagtg cggagaatct cttcgagatc caccacgatg cgggtcgcct
     2581 tggcggggtc aaaagcatcc aagttcggtc gctcgatctg gccggtggaa tcgttcttta
     2641 ccagccgctg ggtattcgcc acaatgtcgg gcaggttctg gcggcggatt tcagccacaa
     2701 cctcttgacg ggtgatgatc gtgcccttcg caaaagcccg ctcggtgaaa aatccggccc
     2761 gtttgaggag cgcgtagagc gtctcgtttt ccttctggag ggtgtattcg cccgggaact
     2821 gcacctcccc ggtaattttg acgttctcct gcagccggta ctggggaatt tcacggatga
     2881 agacctggtc gtcttccttc aggaacaggt tctgcggcga ttccggatcg gcctgcacgt
     2941 cggccaggtt gacgaagagg acatccgcgg gcttaccggg gttcatccgg gccacttcag
     3001 cccgcaaaag gtaggcgctt ttgtcgagat tccccgagag gaagatcaaa tcccacaggc
     3061 gcatgctgtc caaaaggtcg tactttccgg gctggcgcac ttcgccggag atgaaaacga
     3121 acttgggatg ttccacttcc tgccgcgagt aaatccgcag tgagtcacgg tcctgcaaga
     3181 cgaagttcat cgtgctgtcg cccgctaaag ccagatccag attcaccgga atgatggcgc
     3241 gcacccggtt gtgcgtcagg cggtagaggt cggcccggtg ccggtaggcc tcgtattcca
     3301 gaaactcgcc gcctttgagc agatcgcgga tcgtcattcc cgcggtgcgt tcaaaggtgc
     3361 ccggatgctt cacctggccg gtgagccaga ccatattttc gcgcagttcg tacttggaga
     3421 agaccgaaat gtcgtcgccg tcgtgaatcg taaggttgtt ttccgccagc acggtgctgt
     3481 ctcgcaggtt gaggtcgatc aattgccgcc ggtcgccggc atcgatccgg tcgatcatca
     3541 cccgttccag gtaggcgttg gggagcgtcc ccccggccag agcgatgacc gcagtcaagc
     3601 gctcggcgtc tttcagttca tagatcgccg gacgcttgac ctggccccgg attttcacca
     3661 agggccccac caccggcacg taaatcacgt caccggagag aagtttcagg tcgtcgcgat
     3721 tgaagccctt caggagaaac tcgtagaaat cgaccgtggc caccaactgg ttttgccgga
     3781 ccaatcggat ctgcctgaga ctcccccgct cgttcggccc gccggccaga tagagcgcat
     3841 tgaaaaggga agccagggag gtgaccgcat acccgccggg ccttttcact tcgccgaaga
     3901 cgtagacttt gatcgtccgc agtttgccga ggatgacagt caagcggaag ttggaataaa
     3961 cggtggagag tttcgaccgg aggcgctttt cgaattccgg aaccgtaagc ccccacgcga
     4021 tgacctcgcc cgctttggga atgaagactt tcccttcgcg gtcgcaggtg aggttcagtt
     4081 ccagatcgac ccggccccag aggttgacga tgagattatc gccgggaccc agagtgtaat
     4141 cgaccggcgg ggcgatatcc tccaccggtg caaaggacga cggctgagcc gtgaagatat
     4201 cgtaaccgaa cagtttcaat tcttcactgc tggttgccgc aggtttcggg acgggcattt
     4261 tcccttccgg aaaaatcgaa cccttgtccc ccgcattggg ctttaccgca tccggtttcg
     4321 gctcgggaat ggacgagtaa atatccggag tttggtaggt ttgcgtttcc aggagtttgg
     4381 ccgcgtcggg cggcgccccg ccggtggttt gcttcaactt ttcatattcg acgatgagcc
     4441 gcgctttctc ttccggcgtc aggtcctgag caaaggtgga agtgattgcg gcgaacgaaa
     4501 agaaaagcag tcccgccgtt gccaaccacg caagcgtttg ggactgcacc cgccccctat
     4561 tccgccctgg ccgttcagag cgtatgaccg tacgggcagg ccataggcgg aaagacttaa
     4621 agccgtgatt cctattcacg taaacctcct tcttcggcgg tcttaattgg caaaccggat
     4681 gccgcccgta cgggcccgaa gccccttttt gaaaaacctt aactaattgc ctgttaatga
     4741 gatagcagac tggctatcgg tcgaaaccaa taccggacgg gacggatgga aaaacttttc
     4801 cccttgacct acgaattttt ttccagtagc cccgtagccg aggattacat tctcggtccc
     4861 cccttctcaa attgtagtgc gggatggtct acctatcccg cacttttttc atttcacatt
     4921 ccaacatatc ttactcccgt gcagaactgg tcacacccgg gcggcaaact gcgcgaactt
     4981 ggcgccgaaa gtctctccga tgccgaactc ctctccatca tcatttcacc gggaattaaa
     5041 ggcaaatccg ccgaaaaaat cgccgaggat atccttgcca aatttcaatc tttcaaaggt
     5101 atgaccaacc agcccctgga gcggtttctg gaaatcaaag gactggccga tgtcaagatt
     5161 atccgcatcg ccgcctgctt tgaaattgcc cgccggatcg ttgatcaagt cttagctgaa
     5221 aatgaaaagg tctaaaatca ggggaacccc ccgccggatt cccgccgcct ttgccgccac
     5281 cgccatcaag accgagtggt cggcttccgc tgatttgatg cactggatgc gggagataat
     5341 tgaaaaagag gggttggatt tgggccttcc tgatgtagag accgtggggc cggatcggaa
     5401 aagaccagat ttggtcattt atgaaacgcg acgaagcgac cggattctct gcccagttga
     5461 gaccaagcta ccctattttg acgtattcaa cgagtccgaa ctaaaagaac cagctcggaa
     5521 gaaagctaat cagagaaaag ccaaatactt tgccgtaaca aactacaagc agttagtctg
     5581 gtacagcacc accaaggtta acgaaaacgc tccggaggaa gaacaaatca agggaaggta
     5641 tttcctatcc gaaatcgaag acctgaatga aattaaagag gcccgattta gccgtcctac
     5701 catagttgcg ctcaaacagt tccttactga tctctatgaa gttcatactg gcaagaaagc
     5761 ggagccgaaa cacccggtcg acgagtttct ggtctggcgg ctgcacgaaa aaattcgggt
     5821 actggctaag tattattcaa ggattattta tgaacggtat aacgaggacc ctaaattcag
     5881 caaagagctt cgccaatggt tcggggacca gaactggcag tttgctggcc agccatccga
     5941 tttcgataaa gccgcccgcc agaccgctta ccttttggtc aacaaaatac ttttctatga
     6001 cctcctgcat gccaaacgcc ctggcgattt agacccgctc gaaattccag aaggcctgac
     6061 caaaggctcg atgctggccg ccaccctgca gggttatttc agccaggtct taagaattga
     6121 ctacgaaacg atttacgaca ccgatttcat tgacagcgtc gcttttcccg attccaaaga
     6181 agttgtggtt gaaataaaag cgttaacgaa gttgctccgc cgctacgacc tggcccgtct
     6241 gggctacgat atcgttggcc ggatctttga gcgcttgatc ccggaagatg agcgccacaa
     6301 tttaggccag tactttactc gttcggacgt ggttgatttg attcttcgct tttgcttgca
     6361 acacgaaacc gacagagttt tggacccggc ttgtggtgcc ggaacttttc tggtgcgggc
     6421 ctaccagcat aaaaaaatga tgaaccagcg actggagcac gagaaaatac tggaaacgct
     6481 ctggggcaac gatattgcca agttccctgc ccatttagct accatcaact tggcgattaa
     6541 agatttggga tcgaacgaga attatccaag aattttgaag aaggatttct tctccctgat
     6601 agttaaacaa gatggttttg actatgaaaa ttccctcaag ggtgaaattc aaactttgtc
     6661 taaggtaaag cgagaagtga agcatcctcg ttattttgaa gccatcgtcg gcaatccgcc
     6721 ctatacccgg caggaagaaa ttagcgacat ctctcctgga gacaagaaat ataaagaaac
     6781 actcatcaaa agagcccttt tagatgtcac cgaaaggccc ttagcccaca tcggcaaacg
     6841 ggctggcatt cacgcct
//
LOCUS       DBNGZD010000313         8944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1161685, whole genome shotgun sequence.
ACCESSION   DBNGZD010000313 DBNGZD010000000
VERSION     DBNGZD010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1161685"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /gene="rpsK"
                     /locus_tag="AC16GM_08385"
     CDS             complement(<1..261)
                     /gene="rpsK"
                     /locus_tag="AC16GM_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR2923730.1"
                     /translation="MQKPQAKKPKKRQRRVDTNGVVHIKATINNTLVTITDTAGHVIC
                     WASAGKSGFQGSKKSTPFAAQVAAERCARDAMDQGLRRVEVWV"
     gene            complement(277..696)
                     /gene="rpsM"
                     /locus_tag="AC16GM_08390"
     CDS             complement(277..696)
                     /gene="rpsM"
                     /locus_tag="AC16GM_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR2923731.1"
                     /translation="MARIAGVDLPKERRIDAALPYLYGIGWPSARRILARTGINPGTR
                     VHQLTEEEIAKLRAAIESDYKVEGALRSEVAMNVKRLIDIGSYRGLRHRRSLPVRGQR
                     TKTNARTRKGPKKTIGRLRKEAPEPAKVAPTGGEKKG"
     gene            complement(712..825)
                     /gene="rpmJ"
                     /locus_tag="AC16GM_08395"
     CDS             complement(712..825)
                     /gene="rpmJ"
                     /locus_tag="AC16GM_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR2923732.1"
                     /translation="MKVRSSIKKRCEHCKIVRRRGVLFVICKNPRHKQRQG"
     gene            complement(854..1072)
                     /gene="infA"
                     /locus_tag="AC16GM_08400"
     CDS             complement(854..1072)
                     /gene="infA"
                     /locus_tag="AC16GM_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR2923733.1"
                     /translation="MPKEETIQVEGKVLEPLPNAMFRVELDNGHKILAHVSGKMRMHF
                     IKILPGDKVTVELSPYDLSRGRIVYRYK"
     gene            complement(1074..1853)
                     /gene="map"
                     /locus_tag="AC16GM_08405"
     CDS             complement(1074..1853)
                     /gene="map"
                     /locus_tag="AC16GM_08405"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR2923734.1"
                     /translation="MELKSPTEIGLMRRAGASVARTLALVEQLVGPGITTGELNDRAE
                     TLIRQEGGVPAFLGYHGYPKAICVSIDDEVVHGIPGSRRLKSGQIVSVDVGVALDGYF
                     ADGAATFAVGRVSDEAARLLAVTQKALAAGIAQAKAGNRLSDISAAVQGTVEENGFSV
                     VRDLVGHGIGRQMHEEPQVPNFGRPGEGPTLAVGLVLAIEPMVNAGGYAVAVKPDRWT
                     VVTKDGSLSAHFEHTVAITDNGPEILTWFGELTTLSKVAGR"
     gene            complement(1886..2560)
                     /locus_tag="AC16GM_08410"
     CDS             complement(1886..2560)
                     /locus_tag="AC16GM_08410"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR2923735.1"
                     /translation="MWLVLLGPPGSGKGTQAKELASRFGYQSIATGDLLRSEVEKETE
                     LGQAAKKYMDAGALVPDEVILAMLALQIPKALGGVIFDGFPRTVAQAEGLDQVGERLG
                     ARVHLAIKLDVPDDEIIRRLAARRLCPACGASYNTETQPPRTPGCCDHCGAALVSRND
                     DLPEVVANRLAVYRRETTPVEAYYQKRGLLRTVNGNRPRAEVERALEALVAECKKQAA
                     SPTSRP"
     gene            complement(2567..3886)
                     /gene="secY"
                     /locus_tag="AC16GM_08415"
     CDS             complement(2567..3886)
                     /gene="secY"
                     /locus_tag="AC16GM_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR2923736.1"
                     /translation="MIQTFQNIFRIPELRRRVLFTLGLLVIYRLGGHIPSPGIDSAAL
                     GELFRSAGAGTLFGLYDLFVGGAFFKATVFALGIMPYISSSIILQLMGAVVPYFQKLQ
                     KEGEEGRRKLTQYTRYGTVLIAALQALGTAVYLESLRVPGTNALIVPHPGMLFKFLCM
                     ITFSSGTIFIMWLGEQITEKGIGNGISLIIFIGIVARLPQSIVDEVVELSAGRRSVVV
                     ELLLIAIIVGVIAAIVLVTRAQRRIPVQYAKRVVGRRVYGGQSTHIPLSVNTAGIIPI
                     IFAQSILFAPGTLSSFFPNVQFMQDVAAAFRPGAVLYSLVYALLILFFAYFYTAIVFN
                     PIDLADNMRKYGGFIPGIRPGKHTSDYIDRLLSRFTLPGALFFAAIAILPYIFIKQLN
                     VSYYFGGTGLLIVVGVALDTLQQVESHLLMRHYEGFMKKGRIKGRGF"
     gene            complement(3883..4326)
                     /gene="rplO"
                     /locus_tag="AC16GM_08420"
     CDS             complement(3883..4326)
                     /gene="rplO"
                     /locus_tag="AC16GM_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR2923737.1"
                     /translation="MPILPLNPPRGSTKKRKRIGRGPGSGHGKTATHGHKGQKARSGA
                     KFPAHKEGGQMPLARRLPKRGFTNIFKKTFQVVNLRQLSGFKPQEVITPLVLQNRGLV
                     SAVGRPIKILGDGRLLVPVVIQAHAFSRSAREKITASGGRCELVK"
     gene            complement(4332..4529)
                     /gene="rpmD"
                     /locus_tag="AC16GM_08425"
     CDS             complement(4332..4529)
                     /gene="rpmD"
                     /locus_tag="AC16GM_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041570711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR2923738.1"
                     /translation="MEKSKLEITLVRGFSGQIQKQRRILRALGLSRRHQKVLHADSPT
                     IRGMLAKVNHLVQVTDRSGAR"
     gene            complement(4532..5041)
                     /gene="rpsE"
                     /locus_tag="AC16GM_08430"
     CDS             complement(4532..5041)
                     /gene="rpsE"
                     /locus_tag="AC16GM_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR2923739.1"
                     /translation="MRTDRQSRDDQDFFEFEEKVIQVNRVAKVVKGGRRFSFSALVAI
                     GDKRGRVGVGLGKAREVADAIRKGHEEARKALFKVPVAGGTLPFEVTSHYVSSSVLLK
                     PASAGTGVIAGGPVRAVMEVAGIQDVLTKSWGSTNPNNVVKATLQALRRLNDFVEQEQ
                     LRKAEEAKA"
     gene            complement(5038..5412)
                     /gene="rplR"
                     /locus_tag="AC16GM_08435"
     CDS             complement(5038..5412)
                     /gene="rplR"
                     /locus_tag="AC16GM_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR2923740.1"
                     /translation="MILEQERKKERALRRRKRVQGKVAGTALRPRLSVYRSLKHVYAQ
                     IVDDVNRQTITAAASTGKKVRALLAKGAKKTEVAKLLGQELARLAKEKGITQVVFDRN
                     RYRYHGRVKAVADGLREGGVKL"
     gene            complement(5522..6058)
                     /gene="rplF"
                     /locus_tag="AC16GM_08440"
     CDS             complement(5522..6058)
                     /gene="rplF"
                     /locus_tag="AC16GM_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR2923741.1"
                     /translation="MSRVGKNPVPIPPAVTVQVAGDTVTVQGPKGTLARRFHPYVSVA
                     VADGQAVVTRKGESQFHRSLHGLTRALVQNMVVGVTQGYERALSIVGVGYKAELSGKR
                     LALALGYSHPILFKPPEGITLAVTDQTRIVVRGIDKELVGEVAAKLRALRPPEPYKGK
                     GIRYADEEVRRKAGKTTA"
     gene            complement(6065..6460)
                     /gene="rpsH"
                     /locus_tag="AC16GM_08445"
     CDS             complement(6065..6460)
                     /gene="rpsH"
                     /locus_tag="AC16GM_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR2923742.1"
                     /translation="MLTDPIADMLNRIRNAQKARLREVVIPGSRLKRAVGLLLVQEGM
                     LKACTEERTPPQSRLRLQLLYLAGQKNAITGLVRFSRPGRRAYLSAADLERRSRQMGV
                     VVLSTSQGVMTDRQARKLGIGGEALCRVW"
     gene            complement(6527..6712)
                     /locus_tag="AC16GM_08450"
     CDS             complement(6527..6712)
                     /locus_tag="AC16GM_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR2923743.1"
                     /translation="MACKAMMEKAGREPKFKVRQHNRCWRCGRPRAFLRKFGICRICF
                     RQLALAGEIPGVTKASW"
     gene            complement(6721..7311)
                     /gene="rplE"
                     /locus_tag="AC16GM_08455"
     CDS             complement(6721..7311)
                     /gene="rplE"
                     /locus_tag="AC16GM_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR2923744.1"
                     /translation="MTARLKERYKKEVLPALLKRFGYGNPLAAPRLEKVVINLGVGDA
                     IGNAKLLDAAVADLTAIAGQKPVVRRAHRSISNFKLRAGAPIGCSVTLRGERMYEFLD
                     RLVNVAIPRIRDFRGVSPDAFDGRGNYTLGLQEQIIFPEIVYDKVLKVRGMNITLVTT
                     ARTDEESFELLRGMGVPFRSSRTASDRSKAATTEAK"
     gene            complement(7314..7640)
                     /gene="rplX"
                     /locus_tag="AC16GM_08460"
     CDS             complement(7314..7640)
                     /gene="rplX"
                     /locus_tag="AC16GM_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR2923745.1"
                     /translation="MMRIKKGDQVLIVAGDTKGKIGKVLSVLPGRKRIVVEGDKLIKR
                     HTRPSAKVAKGGIIERERTIHVSNVMLYCTKCSKPTRPAYKVAATAEKGRSKTLRLCR
                     HCGEII"
     gene            complement(7654..8022)
                     /gene="rplN"
                     /locus_tag="AC16GM_08465"
     CDS             complement(7654..8022)
                     /gene="rplN"
                     /locus_tag="AC16GM_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR2923746.1"
                     /translation="MIQEYSVLNVADNSGARRVMCFRVLGGTRKRYAVIGDTIVVTVK
                     DAIPGGTVKKSDVCRAVVVRTTRQTRRPDGSYIRFSDNAAVLINEQAEPRGTRIFGPV
                     ARELRDRQFMKIISLAPEVL"
     gene            complement(8019..8324)
                     /gene="rpsQ"
                     /locus_tag="AC16GM_08470"
     CDS             complement(8019..8324)
                     /gene="rpsQ"
                     /locus_tag="AC16GM_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR2923747.1"
                     /translation="MKTALPIPSVRTQRKVRIGRVVSTKMQKTIVVKILRQLRHPDYG
                     RVIRRSTKLYARAEANQCQVGDLVKLAETRPLSRLVRWRLLEVLERASAPKVEVKDE"
     gene            complement(8327..8563)
                     /gene="rpmC"
                     /locus_tag="AC16GM_08475"
     CDS             complement(8327..8563)
                     /gene="rpmC"
                     /locus_tag="AC16GM_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR2923748.1"
                     /translation="MNVEQLRELSREELLQKKHDLEEELFNLRLRRVTRELENPLRLR
                     TLRREKARILTLLRETELRVPTRSAAKPQPELKK"
     gene            complement(8560..>8944)
                     /gene="rplP"
                     /locus_tag="AC16GM_08480"
     CDS             complement(8560..>8944)
                     /gene="rplP"
                     /locus_tag="AC16GM_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR2923749.1"
                     /translation="RRRGLSLRGAEVSFGEYGLKALEPAWITNQQIEAARVALTRFIK
                     RGGKVWIRIFPDKPVTVKPAETRMGKGKGNPEHWVAVVKPGRVLFELEGVTEVTAREA
                     LGLAADKLPIKTRFVARTALFGTPK"
BASE COUNT         1587 a         2913 c         2602 g         1842 t
ORIGIN      
        1 gacccagact tcgacccggc gcaaaccctg gtccatggca tcccgcgcgc agcgctccgc
       61 cgccacctga gcggcaaagg gcgtgctctt cttggaaccc tggaagcccg acttcccggc
      121 cgaggcccag caaatgacgt ggccggcagt gtcggtgatc gtgacaaggg tattgttaat
      181 cgtcgccttg atgtgcacca cgccgttggt atcgacccgg cgctggcgtt ttttgggctt
      241 tttcgcttgc ggtttttgaa tctcttcagc cacgcactat cctttctttt ctccgccggt
      301 cggggctacc ttcgcgggtt ccggtgcttc tttcctgagc cggccgatgg ttttcttggg
      361 tcctttgcgg gtccgcgcgt tggtcttggt cctctgcccg cgcaccggca agctgcggcg
      421 gtgccgcagg ccgcggtacg agccgatgtc gatcagccgc ttgacgttca tcgccacttc
      481 cgagcgcagg gcgccttcga ctttgtagtc cgactcaatc gccgcgcgca gtttcgcgat
      541 ttcttcttcg gtcaactggt gcacccgcgt gccgggatta atccccgtac gggccaaaat
      601 ccgacgggcc gacggccagc cgatgccgta gaggtacggc agggccgcgt caatccgcct
      661 ctccttcggt aagtccactc cagcgatgcg cgccatttgt ttctttcgct cctacccttg
      721 ccgctgcttg tgccgggggt ttttgcagat gacgaaaagc accccgcggc ggcggacaat
      781 tttacagtgc tcacagcgtt tcttaatcga cgagcgtact ttcatgaacg accttcgctc
      841 ccgtgtccca gatctacttg taacggtaga caatccgccc gcgcgacagg tcgtagggcg
      901 agagttccac cgtcacctta tcgccgggga gaatcttaat gaagtgcatt cgcattttcc
      961 ctgacacgtg cgccagtatt ttgtgcccgt tatccagctc tacgcggaac atcgcattcg
     1021 gtaacggttc gagtactttc ccttcgacct ggatcgtttc ttctttcggc atcttaccgc
     1081 ccggcgactt tgctcagggt ggtgagctcg ccgaaccacg tcaggatctc cggaccgttg
     1141 tcggtaatgg ccaccgtgtg ttcgaagtgg gccgagagcg atccgtcttt ggtcaccacg
     1201 gtccagcgat ccggtttcac cgccacggcg tagccacccg cgttcaccat cggctcgatg
     1261 gccagaacca ggccgacggc cagcgtcggg ccctcgccgg gccgcccgaa attcggcacc
     1321 tgcggttcct catgcatctg gcggccgatc ccgtgaccca ccagatcgcg cacgacggaa
     1381 aatccgtttt cttcgacggt gccctgaacc gccgccgaaa tgtcggagag gcggttgccg
     1441 gctttggcct gggcaatgcc ggctgccagt gccttctggg tgacggcgag caggcgcgcg
     1501 gcttcgtcgg aaactcggcc caccgcaaaa gttgccgcac cgtcggcgaa gtagccgtcc
     1561 agggccacgc cgacgtcgac ggaaacgatt tgcccggact tgaggcgccg gctccccgga
     1621 atgccgtgca ccacttcatc gtcaatggag acgcagatcg ctttcgggta cccgtggtag
     1681 cccaaaaacg ccggcacgcc gccttcctgg cggatcaacg tctcggcgcg atcgttcagt
     1741 tcgccggtgg tgatcccggg accgaccagt tgttcgacca atgccagcgt gcgcgccacg
     1801 ctggcgccgg cccggcgcat gagcccgatt tcggtgggcg atttcagctc cattgctgat
     1861 ccgatcaaaa ccgcttgcgc ccgcgtcacg ggcgcgacgt cggcgacgcc gcctgttttt
     1921 tgcattccgc taccaatgcc tccagagccc gctcgacctc cgcccgcggc cggtttccgt
     1981 tcaccgtacg caggaggccg cgtttttgat aatacgcctc caccggggtt gtctcccgtc
     2041 gatagactgc cagccggttg gcgactactt cgggcaggtc gtcattgcgg ctgaccaagg
     2101 ccgccccgca gtggtcgcag caacccggcg tgcgcggcgg ctgcgtctcc gtattataac
     2161 tcgcaccaca ggccggacac aagcgccggg cggctaaacg ccggataatt tcatcatcag
     2221 gcacgtccag tttgatggcc aaatgcaccc gtgcgccgag gcgctctccc acctgatcca
     2281 gaccctcggc ctgcgccacg gtgcgcggga agccgtcgaa gatgaccccg cccaaagctt
     2341 tgggaatctg caaggccagc atcgctaaaa tcacttcgtc cggaaccagg gcgcccgcat
     2401 ccatgtactt tttggccgct tgacccaact cggtttcctt ttcgacttcg ctccgcagca
     2461 aatcacccgt cgcaatactc tggtacccga aacgggacgc caattccttc gcctgtgtgc
     2521 ctttgcccga tccgggcggc cccaagagca cgagccacat ccggcgtcag aacccccgtc
     2581 ctttgatccg tcccttcttc atgaatcctt cgtagtgacg catcaaaagg tgcgactcca
     2641 cctgctgcag cgtatccagc gccaccccca ccacaatcaa aagcccggtg ccgccgaagt
     2701 agtagctgac attgagctgt ttgatgaaaa tgtaggggag gatggcgatg gcggcgaaga
     2761 agagtgcccc cggcagagtg aagcgagaaa gcaaccggtc gatgtaatcg gaggtgtgct
     2821 tccccggccg gatgcccggg atgaagccgc cgtacttgcg catgttgtcc gccaggtcga
     2881 tcgggttgaa gacaatggcg gtgtagaagt aggcaaagaa taagatcaag agcgcgtaaa
     2941 ctaacgaata gagaaccgct cccgggcgga aggccgctgc cacatcctgc atgaactgga
     3001 cattggggaa aaaggaggag agcgtcccgg gagcgaagag gatcgactgg gcaaagataa
     3061 tcgggatgat gccggcggtg ttgaccgaaa gcggaatgtg ggtgctctgc ccgccgtaaa
     3121 cccgccgccc gaccacccgt tttgcgtact gcaccggaat ccggcgctgg gcccgggtca
     3181 ccagaacgat cgccgcaatg acgccgacaa taatagcgat caagagaagt tcgaccacga
     3241 cgctccgccg cccggcggag agctccacca cctcgtccac gatcgactgg ggcaaacggg
     3301 cgacgatgcc aatgaagatg atgagggaaa tcccgttgcc gattcccttc tcggtgatct
     3361 gttctcccag ccacatgatg aagatggttc cggagctgaa ggtgatcatg cagagaaatt
     3421 taaacaacat acccgggtgg ggtacgataa gggcattggt gccggggacc cgcaggctct
     3481 cgaggtagac cgccgtccct aaggcctgca acgctgcgat gagcaccgtc ccgtagcggg
     3541 tgtactgggt gagcttccgc cttccctctt ctccttcttt ctggagtttc tggaagtagg
     3601 ggacgaccgc tcccatgagc tgcaggatga tcgaggaaga aatgtaaggc ataataccca
     3661 gggcgaagac cgtggctttg aagaacgccc cgccgacaaa aaggtcgtag aggccgaaga
     3721 gcgtgccggc gccggcggag cggaagagtt cgcccaacgc cgccgaatcg atcccggggg
     3781 aggggatgtg gccgcccagc cggtagatga ccaaaagacc caaggtgaag agcacccggc
     3841 gcctgagttc cggaatccgg aaaatatttt gaaacgtctg gatcacttaa cgagttcgca
     3901 cctgccgccc gaagcggtta ttttctcccg ggcgctccgg ctgaacgcgt gggcctgaat
     3961 gaccaccggc accaaaagcc gcccgtcccc caagatctta atcggtcggc caaccgcgga
     4021 aaccaggccg cggttctgga gcaccaaagg cgtaatgact tcctggggtt tgaaaccgga
     4081 gagttgtctt aagttcacca cttggaaggt tttcttaaaa atattggtaa agccccgctt
     4141 ggggagtcgg cgggccagag gcatctggcc cccttcttta tgggccggga attttgcgcc
     4201 gctccgggct ttctgcccct tgtgcccgtg ggtggcggtc ttgccgtgtc cggagcctgg
     4261 tccccggccg atgcgctttc tttttttggt cgatccccgg ggcgggttga gtgggagaat
     4321 cggcatgacg gctatcgtgc tcccgaacgg tcggtgacct gcaccaggtg gttgaccttc
     4381 gccagcatgc cccggatggt gggggagtcg gcatgcaaca ctttttggtg ccgtcgggag
     4441 agtcccaagg cgcgcagaat tcgcctctgc ttttggatct ggcccgagaa gccccgcacc
     4501 agggtgattt cgagtttgct tttttccatg actacgcttt tgcttcctcg gctttgcgga
     4561 gttgctcttg ttccacaaaa tcgttcaagc ggcgcaaggc ctgcagggtg gccttaacga
     4621 cattgttggg gttggtcgaa ccccaggact tggtcaaaac gtcctggatg ccggccacct
     4681 ccatcaccgc ccgcaccgga ccgcccgcaa tcaccccggt gccggcactg gccggcttga
     4741 gcagcacact ggaagagacg tagtggctcg tgacttcgaa cggcagggtt ccgccggcga
     4801 ccggaacctt aaagagcgcc ttgcgggcct cttcgtgccc tttgcggatg gcgtccgcca
     4861 cctcgcgcgc ctttcccagc ccgacgccga cgcgcccccg cttgtcacca atcgccacca
     4921 gggcggaaaa ggagaagcgg cggccgcctt tcaccacttt ggcgacccga ttcacctgga
     4981 tcactttttc ttcaaactca aaaaagtcct ggtcgtcccg gctttgacgg tcggttctca
     5041 cagtttcacg ccgccttcgc gcaatccgtc ggccaccgcc ttgacccgtc cgtggtagcg
     5101 gtagcggttg cggtcaaata ccacctgcgt tattcctttc tccttggcca gccgggccag
     5161 ttcctgcccg agcagtttgg ccacctcggt ctttttcgct cccttggcca gcagggcccg
     5221 caccttttta ccggtgctgg ccgcggcggt gatcgtctgg cgattgacgt cgtcgacaat
     5281 ctgagcatag acgtgcttca ggctccggta gaccgaaagc cgcggccgga gggcggtccc
     5341 cgccaccttc ccctgcacgc gcttgcggcg ccggagcgcc cgttcttttt ttcgctcctg
     5401 ctccagaatc atcggcccta cccgctcact cgccggctat tcctgctttc atccctcacg
     5461 gtaatagaac cagcacaacc agaaataggt ttgacacgtt ctatcatctg cgtccgaatt
     5521 tctacgccgt ggtcttcccc gctttgcggc gcacttcttc atccgcgtag cgaatgcctt
     5581 tgcctttgta cggctccggc ggccggaggg cgcgcagctt ggccgccacc tcccccacca
     5641 gttccttgtc gattccgcgc accacgatcc gggtctggtc ggttacggcg agcgttatcc
     5701 cttccggcgg cttgaataaa atcgggtgcg agtaccctaa ggcaagcgca agccttttgc
     5761 ccgagagctc ggctttgtag ccgaccccga cgatggagag ggcgcgctca tacccctgag
     5821 tgacccccac caccatgttc tgcacgagcg cgcgggtgag gccgtgcaag cttcggtgga
     5881 actggctttc ccctttccgc gtcaccaccg cctggccgtc ggcgaccgcc acggaaacgt
     5941 agggatggaa ccgccgtgct aaagttccct tgggtccttg gacggtgacg gtatctcccg
     6001 ctacctgcac ggtgacggcc ggcggaatcg gaaccggatt tttccccacg cgcgacatag
     6061 tgctttacca cacccggcag agcgcttcgc cgccgatccc caacttgcgc gcttggcggt
     6121 cggtcataac cccctgcgag gtcgagagaa ctaccacacc catctgccga ctccggcgct
     6181 ccaggtcggc ggcggaaaga taggcccgcc gcccggggcg ggaaaaacgc accaaaccgg
     6241 taatggcgtt cttctggccg gccaagtaga gcaactgcaa ccggaggcgc gactgcggcg
     6301 gagtgcgctc ctccgtgcag gctttcagca tcccctcctg aaccagaagc aggccgaccg
     6361 cgcgcttgag ccgggagccg ggaatgacca cttcccgcaa gcgcgctttc tgcgcgttcc
     6421 ggattcggtt gagcatgtca gcaatcggat cagttaacat cgcttttacg catctcctgt
     6481 tccagagatt ttttctccgt ccgtcacccg cacgatcgat cggacctcac cagctcgcct
     6541 tggtcacgcc gggaatctcc cccgccaggg ccagctggcg gaagcagatg cggcagatgc
     6601 caaatttgcg caagaaagcg cggggccgtc cgcagcgcca gcagcggttg tgctgccgca
     6661 ccttgaattt cggttcccgt ccggcttttt ccatcatcgc tttacacgcc atcggtacct
     6721 ctatttcgcc tcggttgtgg ccgcttttga ccggtccgac gcggtccggg aggagcgaaa
     6781 gggcaccccc attccgcgca aaagttcaaa gctttcctcg tcggttcggg ccgtggtcac
     6841 gagcgtaatg ttcatcccgc gcaccttcag cactttatcg tagacgattt ccgggaagat
     6901 aatttgctcc tgcaggccca aagtgtagtt cccccgacca tcgaaggcgt ccggcgagac
     6961 gccccggaaa tcccggatcc gggggattgc cacgttgacc aggcggtcta aaaattcgta
     7021 catccgttcg ccgcgcaggg taaccgagca gccgatgggc gccccggctc gcaatttgaa
     7081 gttcgaaatc gaacggtgcg cccggcgcac caccggcttc tgcccggcga tggcggtcaa
     7141 atcggccacc gccgcgtcca aaagctttgc gttgccgatg gcatcgccca cacccaggtt
     7201 aatcacaact ttttccaggc gcggggcggc cagcgggttg ccgtagccga accgcttcaa
     7261 aagcgccggc agcacctctt ttttgtaccg ttccttcaag cgcgccgtca tcgtcagatg
     7321 atttctccgc agtggcggca gagccggaga gttttcgacc ggcccttctc cgcggtggcg
     7381 gcgaccttgt aagccgggcg cgtcggtttg gaacacttgg tacagtagag catgacgttg
     7441 gagacgtgga tggtccgttc cctctcgatg atgccgcctt tggccacttt ggccgacggc
     7501 cgggtgtggc gcttgatcag tttgtcccct tccaccacga tccgcttgcg ccccggcaaa
     7561 accgagagca ccttgccgat tttccctttg gtatcgccgg cgacgatcaa cacctggtcg
     7621 ccttttttga tccgcatcat aggacgttga ccctcacagc acttccggcg ccagtgatat
     7681 gattttcata aactggcggt cgcgcaactc ccgcgccacc ggaccgaaaa tccgggtgcc
     7741 ccgcggctcg gcctgctcgt tgatcaaaac cgccgcgttg tcggagaagc ggatgtacga
     7801 accgtccggc cgccgggtct ggcgggtggt gcgcactacc accgcacggc agacgtcgct
     7861 ctttttgacc gtcccgcccg ggatggcatc tttcaccgtc accacaatgg tgtcgccgat
     7921 cacggcgtac cgcttgcgcg tgccgcccag aacccggaag cacatcaccc ggcgcgcgcc
     7981 ggagttgtcg gccacgttca agacggagta ctcctgaatc actcgtcttt cacctcaact
     8041 ttgggggcac tggcccgctc cagaacttcc aggagccgcc agcgcaccag ccgcgacaaa
     8101 ggcctggtct cggctaattt caccaagtca cccacctggc actggttggc ttccgcgcgg
     8161 gcatacagtt tggtcgagcg ccggatgacc cggccgtaat ccggatgccg cagctgacgc
     8221 agaattttga ccacgatggt cttctgcatc ttggtggaaa cgacccggcc gatgcgcacc
     8281 ttgcgctgag ttcggacgga cgggatgggc agcgctgttt tcatatttac ttcttcaact
     8341 cgggctgcgg cttggccgcg ctccgggttg gcacccgcaa ttcggtttcc cgcaggaggg
     8401 tgaggatccg tgctttttcg cggcgcaggg ttctcagccg caaaggattt tccagttcgc
     8461 gggtgacccg gcgcaaccgg aggttgaaca gttcctcctc caggtcgtgc ttcttctgca
     8521 acagttcttc gcgcgagagt tcgcgcaatt gttccacgtt catttcggtg tcccgaaaag
     8581 cgcggtgcgc gccacgaatc tggtcttgat cggcaatttg tcggccgcca gtcccagcgc
     8641 ttcccgcgcc gttacttctg taacgccttc cagttcgaac agaacccgcc ccggtttgac
     8701 caccgccacc cagtgctcgg ggttgccttt ccccttcccc atccgggttt cggccggttt
     8761 caccgtcacc ggcttatccg gaaaaatccg gatccagact ttcccgcccc gtttgatgaa
     8821 gcgggtcaaa gccacccgcg ccgcttcgat ctgctggttc gtgatccagg ccggctccaa
     8881 ggctttcaac ccgtattcgc cgaaactgac ctcggccccg cggagcgaga gcccgcgccg
     8941 gcgg
//
LOCUS       DBNGZD010000314         3043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_607440, whole genome shotgun sequence.
ACCESSION   DBNGZD010000314 DBNGZD010000000
VERSION     DBNGZD010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_607440"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="AC16GM_08485"
     CDS             complement(<1..1176)
                     /locus_tag="AC16GM_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR2923750.1"
                     /translation="MFTRYHIKTKVAPPRSPQPSKFVVKVDRLKFVRMIAGELKESSS
                     LSEMKGTIKSRPCLYGVYSRPVGGLAPKSSSCVACYRCVIENPGMVRILPNPDLIEKA
                     QQSGLTPKQISTLWYEAATGRVPVKGAGYNGPFSGPGFDAIWTDMSEIVRPTRDGIHG
                     REYISTAVEIGSRPAFVQFDNPGVSGSDNLVHLPIPMLFDATAIPLPYFQRAAAATAR
                     QLATLVFVPAAAAGLPGDGRNIIPLVSISDLEKKSDFFKGFKLLEILCNSVDEYRKVK
                     QATGKMIIARLAITLGIEKLITELLREKVEAIHFSPELPGANGTQPALLRDTLRSVHR
                     ILVQERKRDQVTVIAGGAITAAEHVPKAIICGADAVALDLPLVVALQGEFRSGENGRP
                     "
     gene            complement(1348..2385)
                     /locus_tag="AC16GM_08490"
     CDS             complement(1348..2385)
                     /locus_tag="AC16GM_08490"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HIR2923751.1"
                     /translation="MKTVAASRVTKLLPFSSDARLREAYVNTFGDVRFGLVLEELDHV
                     AGEAAHKHIGRNDLWVVTAAVDRIDLIHPVRIAQDLRFTAQVNYVGRTSLEVGIDIES
                     RVEETWLTAAHAFFTMVAVNQNFVPTPIDPIVPETPEEKQVMEEAKKRKVEYQQSKIL
                     SLERHPPTEEESRILHDLNPASKQSRAVIPMSVTRLENTLFMHPQDKNKFGKVFGGYV
                     MRTAYELAWSAAYKFCRERPLIASVDRINFEKPVEIGALVSFQALVSFVGRTSMGVEV
                     VVKVINPKKEEEIVTHSGYFTLICMDQNRKPIPAPKVIPQNDEEALKYLEGCRRYRRN
                     RDLRTKQKHGA"
     gene            2633..>3043
                     /locus_tag="AC16GM_08495"
     CDS             2633..>3043
                     /locus_tag="AC16GM_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923752.1"
                     /translation="MPLYEFLTKHSRAISATAGLIVIGGFAYGIFKLFFESADLEVIV
                     TVNDANLPTSIASKYSSSMNWFVDRFPKKEVSQDVKKALSDVLEVSSFLTETNSKATF
                     EIDNNSNKTLKNIDIRIKNVSKLTAWGVTGNSLQT"
BASE COUNT          680 a          835 c          814 g          714 t
ORIGIN      
        1 cggtctcccg ttttctcccg atcgaaattc accctggagc gccaccacca aaggcaaatc
       61 gagagcgacc gcatctgcgc cgcagatgat cgccttcggt acgtgctcgg cggcggtaat
      121 cgcccctccg gcgatcacgg tcacctgatc tcttttgcgt tcttggacca aaatccggtg
      181 gaccgaacgc agcgtgtcgc gcaacagcgc cggctgggtg ccgttcgctc cgggcagttc
      241 gggagagaag tgaatggcct cgactttctc ccgcagaagt tcagttatta acttttcgat
      301 gcccagcgtt atggccaagc gggcgataat cattttcccc gtggcctgtt tgaccttccg
      361 gtactcgtcc acactattgc agagtatttc cagaagcttg aaccccttga agaaatcact
      421 ctttttttct aaatcggaga ttgaaaccaa aggaatgatg ttccttccgt cgcccggaag
      481 acccgctgcc gccgccggga cgaaaaccag agtcgccaac tgacgcgccg ttgcggcagc
      541 cgcccgctgg aagtagggca gcggaatggc cgtggcgtcg aaaagcattg ggatcggaag
      601 gtgcacgaga ttgtcggagc ctgacactcc cggattgtca aattggacga acgctggtcg
      661 agaaccaatc tccactgccg tggagatgta ctctcgtccg tggatgccgt cccgcgtcgg
      721 gcggacgatt tccgacatgt cggtccagat ggcgtcgaag ccggggccgg agaaaggccc
      781 gttgtagccg gctcccttca ccggcacgcg gccggtcgcc gcctcgtacc agagggtgga
      841 gatttgtttg ggcgtcaggc cggattgctg ggctttttct atcaaatccg gattcggcag
      901 gatgcgcacc atccccggat tttcgatgac gcagcggtag caggccacgc aggaactgct
      961 tttgggggcc aagccgccga ccggccggct gtagacgccg tacaggcagg gccggctttt
     1021 gatcgtcccc ttcatttccg aaagcgaaga cgactctttc agttccccgg cgatcatccg
     1081 gacgaacttc agccggtcaa ccttcaccac gaactttgac ggctgcggtg accggggtgg
     1141 agctactttc gtcttgatat ggtagcgggt aaacatgctt gctttggctt taacttttat
     1201 agtttgcgat ccgacctgca gttcggatag acttcgcctt catcttctca aaaattgtca
     1261 cgtaacctga agtacaacgc tgaaccctac cgcagcatac atcggaattg aacggaacgc
     1321 aagaaaaatt ctcgaagacg ttctttttca ggccccgtgc ttctgtttag ttcgcaagtc
     1381 tcggttcctt cgataccgtc ggcagccctc caaatatttc agcgcctctt cgtcattctg
     1441 cggaatgact ttcggcgccg gaatcggctt ccggttttga tccatgcaga tcaaagtgaa
     1501 gtacccgctg tgggttacaa tttcctcttc ttttttggga ttgatcacct tgaccaccac
     1561 ctccaccccc atcgaggtgc gcccgacaaa agagaccagc gcctggaaac tgaccaaggc
     1621 cccgatttcg accggctttt cgaagttgat gcgatcgacg gatgcgatga gtggccgttc
     1681 gcggcaaaac ttgtaggccg ccgaccaggc cagttcgtag gcggtgcgca tcacgtatcc
     1741 cccgaacact ttcccgaatt tgttcttgtc ctgcggatgc atgaagaggg tgttctccag
     1801 gcgcgtcacg gacatcggaa tgaccgcacg gctctgtttt gaggccgggt taagatcgtg
     1861 cagtatccgg ctttcttctt ccgtcggcgg gtggcgttcg agagagagta ttttgctctg
     1921 ctgatattct actttgcgtt tcttcgcctc ttccatcacc tgcttttctt ccggagtttc
     1981 ggggacgatt gggtcgatgg gtgtcgggac gaaattctga ttcaccgcca ccatcgtaaa
     2041 aaaagcgtgt gcggcggtta gccaggtttc ttcgacgcgg gactcgatgt cgatgcccac
     2101 ttccagcgac gtgcggccga cgtagttgac ctgcgcggtg aaccgcaaat cctgggcgat
     2161 gcgtaccgga tgaatcaggt caatcctatc cactgctgcg gtgacgaccc agaggtcgtt
     2221 gcggccgatg tgcttgtgag ccgcctcccc cgccacatgg tcgagttctt ccaacaccag
     2281 cccgaagcgc acgtcgccga aggtgttgac gtacgcctcg cgcaggcgtg cgtcggaaga
     2341 aaagggcaga agtttggtga cccgggaggc ggcaacggtt ttcatcgctc gaccggataa
     2401 tacccccaac tcgtaagtgg acgcaaggaa gaacttgaac cttcaaggaa tacttgattt
     2461 tggtcatacc atgccttaac ttttccgggt gtgaatattc acgggcgggg cataacgacc
     2521 caacgcacct ttatacgaaa ttcctatatg gaatagcaaa accttaagca cgccaggcat
     2581 cagatgagat ttgcacaaag gaatgttcgt caaacactaa ttcggacggt atatgccact
     2641 atatgaattc ctaacaaagc attccagggc aatttcagca acagctggtc tcatcgtcat
     2701 aggcggtttt gcttatggca ttttcaaatt gttttttgaa tcagcggatc ttgaggtgat
     2761 agtaacggta aacgatgcta acctgccaac ctcaatagcc tcaaagtact cgtcaagtat
     2821 gaactggttc gtcgaccgct tcccgaagaa ggaagtcagt caagatgtca aaaaggcact
     2881 cagtgatgta ttggaagtgt caagtttcct aactgaaacg aacagcaaag cgacatttga
     2941 gattgacaac aactcaaaca agacgctaaa gaacatagat atccggatca agaacgtcag
     3001 caaacttaca gcatggggag taactggtaa ttcgctgcaa acc
//
LOCUS       DBNGZD010000315         2465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_745893, whole genome shotgun sequence.
ACCESSION   DBNGZD010000315 DBNGZD010000000
VERSION     DBNGZD010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_745893"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="AC16GM_08500"
     CDS             <1..566
                     /locus_tag="AC16GM_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HIR2923753.1"
                     /translation="AYGYLKAESGVHRLVRISPFDANKRRHTSFASVFIYPEIEYNIE
                     IDIKDEEIRVDTYRASGAGGQHVNKTSSAVRITHLPTGIVVQCQTERSQHRNRESALK
                     VLRARLYQFHKEKEEEKFLAIEKSKKKIEWGSQIRSYVFHPYTLVKDHRTEHETSNVQ
                     AVMDGEIDDFIQAFLAHKNETPLVTGA"
     gene            568..1059
                     /locus_tag="AC16GM_08505"
     CDS             568..1059
                     /locus_tag="AC16GM_08505"
                     /inference="COORDINATES: protein motif:HMM:NF017774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR2923754.1"
                     /translation="MSHTVLLVDGYNLIGQTNALKAQANLAVGRERLIERLSDLAFQG
                     NYKVLLVFDAQYQGQAWVQKARERQVTVLFTQKGQTADQFIVTWLKKRPHRGGVIVIT
                     RDRELVAVLRKLRVRILPDLSSLEAAIADGAGLISHQSQPHLVGEQLSAKSFEALRGV
                     VRK"
     gene            1061..>2465
                     /locus_tag="AC16GM_08510"
     CDS             1061..>2465
                     /locus_tag="AC16GM_08510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2923755.1"
                     /translation="MTGSTPATSPASYKRVKGIFGPDRVLSDPGQLETYSSDATKLTY
                     LPDLVVFPANADELQKLIRVAMAEKIPLVPRGAGTGYTGGALAVKGGIVISFEKMNHI
                     LGINAERRIVFTEPGVITYEIAQAAQKAGLFYPPDPASIKISTIGGNVAECAGGLRCK
                     KYGVTKQYVLGLEFVAGTGEIYRTGVLTENGGLGFDFTALLTGSEGTLGFITRIALKL
                     INPPEARRTFLATFASTEKAAAVVAEFTRQGVVPSLVEFVDQDALAITLTYLHKSGFT
                     DAAALLLVEVDGSAGAVEQESGYVLNILQMHALEVRQAVTPEEVEELWLVRREVSTAQ
                     RNLFRTKISEDICVPPSRLPEVVAFQKVLAERFGLRVNSFGHAGDGNLHVNFLTNGGV
                     PEERSRIETALEELFDKTLELGGTISGEHGIGVTKSQYLEREVGQAGLELFRKMKAVF
                     DPLGVVNPGKIFFDATVG"
BASE COUNT          545 a          705 c          742 g          473 t
ORIGIN      
        1 atgcctacgg ctacctcaag gccgaatcgg gcgtccaccg tctggtgcgg atttcaccct
       61 tcgacgccaa caagagacgg cacacctcgt ttgcctcagt tttcatctac ccggaaattg
      121 aatacaacat tgaaatcgac atcaaggacg aagagatccg agtggacacc taccgtgcct
      181 cgggtgcggg cggccagcat gtgaacaaaa cctcttcggc cgtgcgcatc acccacctgc
      241 ccaccggcat cgtggtgcag tgccagaccg agcgctccca gcaccgcaac cgcgagagcg
      301 cgctgaaggt gctgcgcgcg cggctgtacc agttccataa ggaaaaggaa gaggagaaat
      361 ttttggcgat cgagaagtcg aagaagaaaa tcgagtgggg gagccagatc cgctcctacg
      421 ttttccatcc gtacacgctg gtgaaagacc accgcaccga gcacgaaacc tccaacgtcc
      481 aggcggtgat ggacggggaa attgatgatt tcattcaagc ctttctggcg cacaaaaacg
      541 aaacgccgct ggtgaccgga gcctgagatg tcccacaccg tgctcttagt tgacggctac
      601 aacctgatcg ggcaaacgaa cgcgctcaaa gcgcaggcca atctggccgt cgggcgggag
      661 cggctgattg agcgcctctc cgacctcgcc tttcagggca attacaaggt tctcttggtt
      721 tttgacgccc agtaccaagg acaggcctgg gtgcagaagg cgcgtgaacg gcaggtgacc
      781 gtcctcttca cgcagaaggg acagaccgcc gaccagttca tcgtcacctg gctcaagaaa
      841 cggccgcacc ggggcggggt gattgtcatc acccgcgacc gcgaactcgt ggccgtgctg
      901 cgcaagttac gggtgcgcat cctgcccgac ctttccagtc tggaagcggc cattgccgac
      961 ggcgcgggcc tgatctcgca tcaaagccag ccgcacctgg tgggcgagca gctctccgcc
     1021 aagagttttg aggccctgcg cggtgtcgtc cgaaagtgaa gtgaccgggt ctacccccgc
     1081 aacttctccg gcctcctaca aacgtgtcaa agggatattt ggaccagacc gggtgctttc
     1141 cgacccgggg cagttggaaa cctattccag cgacgccacc aagttaacct acctgcccga
     1201 cctcgttgtt ttcccggcca acgctgacga actccagaag ttaatccgag tagctatggc
     1261 ggaaaagatt ccgctggttc cgcgcggggc gggcaccggt tacaccggcg gggcactggc
     1321 ggtcaaaggc gggattgtga tcagttttga gaaaatgaac cacatcctgg gcatcaatgc
     1381 ggagagaagg attgttttta ccgaacccgg cgtcatcacc tatgaaattg cgcaggccgc
     1441 tcaaaaagcc gggctctttt atccccccga cccggcctcg attaaaatct ccaccatcgg
     1501 cgggaacgtg gccgaatgcg ccggcgggtt gcgctgtaag aaatacgggg tgaccaagca
     1561 gtacgtcctc gggttggagt ttgtggcagg tacgggcgaa atttatcgaa ccggcgtgct
     1621 gaccgagaac gggggtctcg gtttcgactt caccgcgctt ctgaccggct cggaaggaac
     1681 gctggggttc atcacccgga ttgcgctgaa gttaatcaac ccgccggaag cgcgccggac
     1741 ttttctggcg acgtttgcct ccaccgaaaa ggcggcggcg gttgtggcgg agtttacccg
     1801 gcagggagtg gtaccttcac tggttgaatt cgtcgaccag gatgcgctgg ccattaccct
     1861 gacgtatctg cacaagtccg gcttcacgga cgcagccgca ctcctcttgg tcgaagtgga
     1921 cgggtcggcc ggcgcggtgg aacaggaatc gggttacgta ttgaacatcc tgcaaatgca
     1981 cgcactcgaa gtccgacagg cggttacgcc ggaagaagtg gaagaactct ggttggtgcg
     2041 gcgggaagtc tccaccgccc agcggaacct ttttaggacc aaaatcagcg aggacatctg
     2101 cgtcccgccc agccgcctgc cggaagtggt ggctttccaa aaagtgttgg ccgaacggtt
     2161 tggactgcgg gtcaactcgt tcggtcacgc gggtgacgga aatctccacg tcaactttct
     2221 cacgaatggt ggggtccccg aagagcgatc ccggattgag acggcgctcg aagaactctt
     2281 tgataaaacg ctggaactgg gaggaacgat ttccggcgaa cacgggatcg gcgttaccaa
     2341 gtctcagtat ttggaaagag aagtcggcca ggccgggctg gaattatttc gaaaaatgaa
     2401 agcggtcttt gatcccctcg gtgtggtgaa cccggggaaa atcttctttg atgccacagt
     2461 gggga
//
LOCUS       DBNGZD010000316         3343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_51042, whole genome shotgun sequence.
ACCESSION   DBNGZD010000316 DBNGZD010000000
VERSION     DBNGZD010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_51042"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..852)
                     /locus_tag="AC16GM_08515"
     CDS             complement(169..852)
                     /locus_tag="AC16GM_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923756.1"
                     /translation="MKKVLGVALLVISFIGLSLPVTAQGQGINFSARFGGMLPTNGEF
                     TSAGSLSDEFDLGLMYGAQVGLNLMPSFAVVAGYDFASLPMSVVPTTGAVPKLSIPHF
                     FVKGHYNFTSLIKNPLNRIKPYLSFGGGLYSWKVTADGGSGNQVFGNGTQLKKTSFGL
                     NFGPGFELSLSNSVSLFAEGTYHLVFAKDNSFSGSVTPGNFSVVSGLDNFGFFAINGG
                     LTFSLPMMK"
     gene            1074..1526
                     /gene="rplM"
                     /locus_tag="AC16GM_08520"
     CDS             1074..1526
                     /gene="rplM"
                     /locus_tag="AC16GM_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR2923757.1"
                     /translation="MLRTKKTMKTYVPKVSDVPRQWRLIDAQGQILGRLATRVARLLR
                     GKDKPQFTPHLDLGDPVVVVNARGVRLTGRKESQKTYYWNTGYPQGSRQISFLKYRRE
                     KPEELFRDAVWGMLPHNALGRKLIKKLFVYRDGKHPHQAQKPEPLKLG"
     gene            1542..1934
                     /gene="rpsI"
                     /locus_tag="AC16GM_08525"
     CDS             1542..1934
                     /gene="rpsI"
                     /locus_tag="AC16GM_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR2923758.1"
                     /translation="MAGEVFAAIGRRKTSVASVRLRSGNGQFLVNGQALKEYLGRETS
                     VMVVLQPLEMLGLLGKLDIFCKARGGGLSGQAGAIRLGVARALQQMDPETRKKLKAAG
                     FLTRDPRMVERKKYGRVKARKRYQYSKR"
     gene            2051..2797
                     /gene="rpsB"
                     /locus_tag="AC16GM_08530"
     CDS             2051..2797
                     /gene="rpsB"
                     /locus_tag="AC16GM_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017796699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR2923759.1"
                     /translation="MTAVDLPALLEAGVHFGHQTRRWNPKMKKFIFTAKNGIYLIDLE
                     KTLLCLDRACAKVREVVTNGGSLLYVGTKRQAAELMKQEALRAGMYYVTERWLGGMLT
                     NFQTIRQSIKRLKDLERMRGDGTFEKLPKKEVLGLEKEITQLEKVLGGVKEMGRLPYL
                     VYVIDTKKEKIAVAEANKLGIPVIAIVDTNSDPDVIDFPIPGNDDSFKAIEMITRQIT
                     DTAAEIRTAREKEKEAARQREEKAARAVAS"
     gene            2806..>3343
                     /gene="tsf"
                     /locus_tag="AC16GM_08535"
     CDS             2806..>3343
                     /gene="tsf"
                     /locus_tag="AC16GM_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR2923760.1"
                     /translation="MAVTANLVKELREKTGTGMMDCKKALEVTGGDLEKAVVYLREQG
                     VAQAAKKVGRATREGTIYAALAPDYTAGTMVEVNCETDFVARTAEFRALVEGIAREVS
                     ALDSDIASEKQLPVAEFLQRPLSSQDGRTVGDLLTEGIARLGENLGLKQFARFSTEAG
                     LVFAYIHPGDKLGVLVELA"
BASE COUNT          787 a          949 c         1025 g          582 t
ORIGIN      
        1 ccccgcttct taaactccgg ctttcaacca tttcctccag ctctaaggcg ttcgatcctc
       61 gctcgctttg agcagggttg aagtactctc ttgcaaatag cgaaatggaa aaactgcacc
      121 aataaaaaaa ccccaccgcc cggcggggtt tttggggaga ctttgacgct acttcatcat
      181 cggcaacgaa aaggtcagcc cgccgttgat ggcgaagaaa ccgaagttat cgagaccgga
      241 gaccaccgag aagttcccgg gggtgacgct gccggagaaa ctgttgtcct tggcaaaaac
      301 gaggtggtag gtcccttcgg caaaaaggga aaccgagttg gagagcgaca gctcaaaccc
      361 ggggccgaag ttcaacccga aactggtctt cttcaactga gttccattcc cgaaaacctg
      421 gttgccggag ccgccgtccg cagtcacctt ccaggaatag aggccgccgc cgaaggagag
      481 atacggcttg atccggttga gcggattctt aatcaaggaa gtgaagttgt agtgcccttt
      541 aacgaagaag tgcggaatcg acagcttcgg cacggcaccg gtcgtcggga cgaccgacat
      601 cggcagcgac gcaaagtcgt acccggccac taccgcgaag gacggcatga ggttcaaacc
      661 cacctgcgct ccgtacatca gtcccaggtc gaactcgtcg gaaagcgatc cggcggaggt
      721 gaactcgccg ttggtcggga gcattccccc gaagcgggcc gagaaattga tcccctggcc
      781 ctgcgcggtg accggcaggc tcaatccgat gaaactgatc acgagcagcg cgacacccag
      841 tacttttttc attgacaacc ctcctttccg ctttgcggtt atttcgatta tcggtaaggc
      901 cggcagaaac ttaaccctgt cgaatgccgc ccaaagtgac ggctggttac tctatccata
      961 gcccgaaaaa gtacacccga ttcctccagc gtttcccggc cgggcggctc ttgacagttt
     1021 gagccgcgat tgtatattgg gaaatctttt cgagggttat ccccttgaat gcaatgttga
     1081 ggacgaagaa gacgatgaag acctacgtgc cgaaagtgag cgacgtgccc cgccagtggc
     1141 gcttgattga tgcccagggg cagattttag gccggctggc cacgcgggtg gcccggctgt
     1201 tgcgcgggaa ggacaagccg cagttcacac cgcatctcga tctgggcgac cccgtggtgg
     1261 tggttaacgc ccgcggggta cggctgaccg gacggaagga atcccagaag acctactact
     1321 ggaacaccgg ctacccccag ggttcgcggc agatcagttt cctcaagtac cggcgggaga
     1381 agccggagga actgttccgc gacgcggtct ggggcatgct cccgcacaac gctttgggac
     1441 gaaagttaat caagaagctt tttgtttacc gggatggaaa acatcctcac caggcccaga
     1501 aaccggaacc gctgaagctc gggtaagcac aaggaggcag tttggcaggc gaagtctttg
     1561 cagcaattgg gcgacggaaa acttcggtgg caagcgtccg gctccgatcc ggcaacggcc
     1621 agttcttagt caacgggcaa gcgttaaaag agtatctggg ccgtgaaacc tcggtcatgg
     1681 tggtgctcca gcccctggaa atgttggggc tcttggggaa gctggacatt ttctgcaaag
     1741 ccagaggcgg cggcctctcc ggccaggccg gagccatccg gctcggcgtg gcgcgcgccc
     1801 tgcagcaaat ggatcccgag acgcgaaaga agttgaaagc tgccggcttt ttgacccgtg
     1861 acccgcgcat ggtggagcgc aagaagtacg gacgggtcaa ggcccgcaag cggtaccagt
     1921 actcgaaacg ttaaagcgag tgggaaggaa aggaaaaact cacacagact gattccggtg
     1981 cgggtccccc gcctgtggcg ggggtgcagc ggaaggtgac gtccgtggcg gcccaacccg
     2041 aaaggacaaa atgaccgcag tcgatctccc cgcactcctc gaagccgggg tccacttcgg
     2101 gcaccagacc cgacgctgga accccaagat gaaaaagttc atttttaccg ccaaaaacgg
     2161 catctacctc atcgatctgg agaaaacgct gctctgtttg gaccgggcct gcgccaaggt
     2221 gcgggaagtg gtcaccaacg gcggcagcct cctttacgtg ggcaccaaac gccaggcggc
     2281 ggaactgatg aaacaggaag cgctgcgggc cggcatgtac tacgtcaccg agcgctggct
     2341 gggcgggatg ctcaccaatt tccagacgat ccggcagagc atcaaacgct tgaaggacct
     2401 ggagcggatg cgcggtgacg gcacctttga aaaactcccc aaaaaagaag tcctggggct
     2461 ggaaaaagaa atcacgcagc tcgaaaaagt gctgggcggc gtgaaggaaa tgggccgcct
     2521 gccctactta gtctacgtga tcgacaccaa gaaagaaaaa atcgcggtgg cggaggcgaa
     2581 caagctgggc atcccggtga ttgcgatcgt ggacaccaac tccgatccgg acgtgattga
     2641 tttccccatt cccggtaacg acgattcgtt caaagccatt gaaatgatca cccgccagat
     2701 caccgacacc gccgcggaga tccggaccgc ccgggagaag gagaaagaag cggcccggca
     2761 aagggaagaa aaagcggccc gcgcggtcgc cagttgaggt tggagatggc ggttacggcc
     2821 aatctggtca aagaactccg cgagaaaacc ggcaccggga tgatggactg caagaaggcg
     2881 ctggaggtga ccggcggtga tctggaaaaa gcggtggtct acctccggga gcagggagtg
     2941 gctcaggccg ccaagaaagt gggccgggcg acccgggaag gcacgatcta cgccgccctc
     3001 gccccggact acaccgccgg cacaatggtg gaagtgaact gcgagaccga cttcgtcgcc
     3061 cgcacggcgg agttccgcgc cttagtcgaa ggcatcgccc gcgaggtaag cgcgctggat
     3121 tcggacattg cctcagagaa gcaattgccg gtggctgaat ttctccagcg gcccctctcc
     3181 agccaggatg gccggacggt gggtgacctt ttgaccgaag gcatcgcgcg cctcggagaa
     3241 aacctggggc tcaagcaatt tgcccgtttc tcgactgaag ccggcctggt cttcgcctac
     3301 atccatcccg gggacaaact gggcgtcctg gtggaactgg cga
//
LOCUS       DBNGZD010000317         3472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1579796, whole genome shotgun sequence.
ACCESSION   DBNGZD010000317 DBNGZD010000000
VERSION     DBNGZD010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1579796"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="AC16GM_08540"
     CDS             complement(<1..394)
                     /locus_tag="AC16GM_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2923761.1"
                     /translation="MKINMNRAVQVTYFLLRVVAGLLFLQAGGLILFGWFGGMPGHPG
                     APSLISQTGIGGVLEFFGGIAIMLGLLTRPVAFILSGEMAVAYWQFHAPNGSWPLQNQ
                     GVPAVLFCFIFLYMAARGGGDWSLDALIR"
     gene            complement(424..819)
                     /locus_tag="AC16GM_08545"
     CDS             complement(424..819)
                     /locus_tag="AC16GM_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR2923762.1"
                     /translation="MGIIPKNVDAYLATVPEDKRAALERLRVIIKSVAPEAVEVICYQ
                     IPVFKYQGKFLVGFGAAKNHCALYGMSHVLKAHSKELKPYNTSKGTVRFPVGRPPPAA
                     LVRKLVKARIAEIEAAPKSKEKNYGRKKR"
     gene            complement(903..1154)
                     /locus_tag="AC16GM_08550"
     CDS             complement(903..1154)
                     /locus_tag="AC16GM_08550"
                     /inference="COORDINATES: protein motif:HMM:NF046224.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6958 family protein"
                     /protein_id="HIR2923763.1"
                     /translation="MNIDSSRPGELAGMTFKELVSSVSHNVRKELFPKPGSVSRYTKV
                     VQLDLEANGLIERIPGEKPQRIRRMDGRRRRRASVLGGH"
     gene            complement(1151..1576)
                     /locus_tag="AC16GM_08555"
     CDS             complement(1151..1576)
                     /locus_tag="AC16GM_08555"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2923764.1"
                     /translation="MTLEITVERTFPYPPELVWRALTESAALGEWLMPNDFVPRLGHK
                     FQFRSKPMPGWRGYVECEVIELIAPRRLAYSWLGDGDWKEPTVVRWTLEPVEKGTRLR
                     LEHTNLQEPWGRQLRAMLSQGWKKMIEVRLVQVLERLSA"
     gene            complement(1694..2182)
                     /locus_tag="AC16GM_08560"
     CDS             complement(1694..2182)
                     /locus_tag="AC16GM_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2923765.1"
                     /translation="MQKITPFLWFDNQAEEAAKFYTSVFKNSKIVSTTRYDEAGAKAS
                     GRPKGTAMVVAFELDGQKFTALNGGPHFKFTEAVSFVINCKTQEEVDYFWEKLSAGGK
                     ESQCGWLKDKYGLSWQVVPTILIELLQDKDAAKAKRVMEAMLKMSKIDIKTLKQAYDK
                     KE"
     gene            complement(2191..2640)
                     /locus_tag="AC16GM_08565"
     CDS             complement(2191..2640)
                     /locus_tag="AC16GM_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2923766.1"
                     /translation="MSLIKLKSEPVIIERILSAPAASVWKALTDREQMKQWYFDLAEF
                     KPEVGFEFQFWGGTEEKKYLHLCRITEVIAIKKLAYTWRYDGCAGNSLVTFELFADGN
                     KTRLKLTHEGLETFPQDDPNLAKENFVEGWTDILGTTLKHFVEKQTA"
     gene            complement(2632..3147)
                     /locus_tag="AC16GM_08570"
                     /pseudo
     CDS             complement(2632..3147)
                     /locus_tag="AC16GM_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017860050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            complement(3128..3466)
                     /locus_tag="AC16GM_08575"
     CDS             complement(3128..3466)
                     /locus_tag="AC16GM_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR2923767.1"
                     /translation="MDRTFSALSDPTRRAILRRLASRERTVTELAEPFDVYLPAISKH
                     LRVLEGAGLLMQEKDGRIRRCQLSARPLQDAARWIARYRRFWEQSFDRLDMYLYELQK
                     KEKKCDRKKK"
BASE COUNT          745 a         1004 c          870 g          853 t
ORIGIN      
        1 gccttatcag cgcgtcaaga ctccaatcgc cgcccccccg cgcggccatg tataggaaga
       61 tgaagcagaa caggacggcg ggcacccctt ggttctggag cggccacgag ccgttcggcg
      121 cgtgaaactg ccaatacgcc accgccattt cgccggacag gatgaacgcg accggccggg
      181 tcaggagacc gagcatgatc gcgataccgc cgaaaaactc aagcaccccg ccgatacccg
      241 tttgggatat gagagatggt gccccggggt gaccgggcat tccgccaaac cagccgaaca
      301 ggatcaggcc gccagcttgg aggaacagca gacccgccac gacccgaagc aaaaagtacg
      361 tgacctgaac ggcccggttc atgttaattt tcatcgccgc ctcccgattg ggttgctgtt
      421 gcttcaccgt tttttacgac cataattctt ctcctttgat tttggggccg cctcaatttc
      481 cgcgatgcgg gctttgacga gctttctcac aagcgcggcg ggaggcggcc tgccgactgg
      541 gaagcggact gtcccctttg aggtattata gggtttgagc tctttactgt gcgctttcag
      601 gacgtggctc attccgtaga gcgcgcaatg gtttttcgcc gcaccaaaac ccacgagaaa
      661 ctttccttgg tatttgaaaa ccggaatctg atagcatatg acctcaacgg cttcgggcgc
      721 cacggactta attatcaccc gaagcctttc gagcgcggct cgcttatctt ccgggaccgt
      781 cgcgaggtag gcatccacat ttttgggtat tatccccatg attttcctcg gcggaaataa
      841 tctcttcctc cggcaaatgt caggccacgc gggcgtgagc agaccgtgtc ggccgagcca
      901 acctaatgcc ctccgagcac ggaagctcga cggcgacgtc ttccgtccat tcggcggatg
      961 cgctggggtt tctcaccggg tatcctctcg atcagaccgt tggcttcaag gtcgagctga
     1021 acaaccttgg tgtaccgcga tacagaaccc ggtttcggaa aaagttcctt ccgtacattg
     1081 tgtgacaccg aggacaccag ttccttgaag gtcatcccgg ccagctcgcc ggggcgagat
     1141 gagtcgatat tcatgcactc aatcgctcaa gaacctgaac cagccttacc tcgatcatct
     1201 tcttccagcc ctggctgagc atggcccgca actgacgtcc ccaaggctcc tggagattcg
     1261 tatgctccag acgaagccgt gttcctttct caacgggttc cagcgtccag cggacgacgg
     1321 tcggttcctt ccaatccccg tctcccaacc aactgtaggc caagcgtcgg ggggcgatca
     1381 gttcaatgac ttcgcactcg acataaccgc gccagccggg catgggtttg gaacggaact
     1441 ggaacttatg accgagccgg ggcacaaagt cattcggcat caaccactcg cccagcgctg
     1501 cgctttcggt cagagcacgc cagaccagct ccggcgggta cggaaaagtc cgctcgacgg
     1561 tgatttcaag ggtcatgggt tcttttccac atatttggtg aagcattccc gactccggtt
     1621 ccacctttct cccattcggg ccagcacgga aagatagact tccacgtctt ttgaagaggt
     1681 gcctgccgcg ttcttattct tttttgtcat acgcctgctt taaggttttt atgtcaattt
     1741 tgctcatttt aagcatggct tccatgaccc ttttcgcttt ggcagcatcc ttgtcctgca
     1801 acagctcgat taagatagtg gggacgacct gccacgacag gccatatttg tctttaagcc
     1861 agccacattg actttccttc ccgcctgcgg agagcttttc ccaaaaataa tccacttcct
     1921 cctgcgtttt gcagtttatg acgaacgata cggcttcagt gaatttgaaa tgcgggccgc
     1981 cattcaacgc ggtgaatttt tgcccatcga gttcaaaggc aacaaccatc gccgttcctt
     2041 ttggcctgcc ggaagcttta gcccccgctt cgtcatagcg ggtggtgctc acaatttttg
     2101 aattcttaaa aacggaggtg taaaatttcg ccgcctcttc ggcctggtta tcgaaccaca
     2161 agaacggggt gattttttgc atggagttcc tcaagcggtt tgtttttcca caaaatgttt
     2221 cagggttgtg ccaaggatat cagtccatcc ctcgacaaaa ttttcttttg ccagattcgg
     2281 atcgtcttgg ggaaaagttt ccaagccttc gtgcgtcagt ttgagccgcg ttttgtttcc
     2341 atcggcaaac aattcaaatg tgacgagaga atttcctgcg cagccatcgt agcgccaggt
     2401 gtaggcgagt tttttgatcg caatcacttc ggttattcgg cacaaatgca agtatttttt
     2461 ctcttccgtc ccgccccaaa attgaaattc aaaacctact tcgggcttga attccgccaa
     2521 gtcaaaatac cattgtttca tttgctccct gtccgtaagg gctttccaaa cactcgctgc
     2581 cggagcgctc aaaatgcgtt caatgatcac cggttcgctt ttcagtttaa ttagactcac
     2641 agccagttta tcgaaagact cattccagcc gacaccggca gatttgatca tctctccagc
     2701 cgggagcccg gcgtgcgtca gggtcatttt ggtcttgttt cctttatact tctcgaacgt
     2761 caccgtgacc agcatctcca gcggaaaatc tgcgggcatg ccatagtgcg tagccggcac
     2821 gacattgccc tgctcatcgg caaaacagtc cgtgcagacg attttctcca ttggcacgat
     2881 ttctttatat acgccggtgc tccagatgcc tttctgccag gcctccggcc cgctgtccga
     2941 tcgcatgcag aagagatatt taccgccaac gcgaaaatca atctggcagg agggcgaggt
     3001 gaagatctta ggtccccacc accgcatggc ttctttggga tcagtccagg ccttccacac
     3061 cagctcgcgc ggtgcgtcga aaattcgcgt gatgaatagc tcccgctcag cggagtgcgt
     3121 ggcgggacta tttcttcttg cggtcacatt tcttctcctt tttttgcagt tcgtagagat
     3181 acatgtccaa acggtcgaaa ctctgctccc agaaacggcg atagcgggca atccaccggg
     3241 cagcgtcctg taaaggtctg gctgatagtt gacaacggcg aatccggcca tctttttctt
     3301 gcatcagaag cccggctcct tccaacaccc gcaaatgttt ggaaatagca gggaggtaaa
     3361 catcaaacgg ctccgccagt tcggtaaccg tacgctctct gctggccagc cgtcgcaaaa
     3421 tggcccggcg ggtcgggtcg gacaaagccg aaaaggttct atccagagca tt
//
LOCUS       DBNGZD010000318         6065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_815830, whole genome shotgun sequence.
ACCESSION   DBNGZD010000318 DBNGZD010000000
VERSION     DBNGZD010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_815830"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16GM_08580"
     CDS             complement(<1..195)
                     /locus_tag="AC16GM_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR2923768.1"
                     /translation="MGAAPSASLDQIIDPNDYVVGPGDVLVLGVWGSFEQTQSLVVTP
                     EGTLLIPTLGEITVGGKTLAH"
     gene            complement(603..2438)
                     /locus_tag="AC16GM_08585"
     CDS             complement(603..2438)
                     /locus_tag="AC16GM_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923769.1"
                     /translation="MVVPGHAEKAVRFSSQLVENYRGNPPQAGVIDRREFVCPYNVRV
                     NGLAGNFIFDLTVTPISKKMIGLELELFTFGGQPEHFLRIDTLNWGETLTLASLPLKG
                     RSTLQWTLVPSPAETTFECDTSPLDSTFWNYEGSPRFIFYYRLWSLGDFQWNRWRDNL
                     EDELDALQRTFDLTTPERVRFYYFPCQTKALVWDRRFPFAVDPLRYRVCAVHQPGKSG
                     VDPFPVILEKICHLWGYAPALLAEGLAGYLDYSHYYAQQDLRAGRLLKLATLSKSQDY
                     YAQPAKEATIQAASFVRYLATTYGGARLEQLYRQATDLSLDSTCTAVYGKPLEGLEAE
                     WRAYLDTFPALKPDLNHFAERALRFGHYRDALELFQKLVALDSTDEYEVGQLVLTHYL
                     LGQYPQGLKLALTNLHFHPTAENQVVAANFFLLADQLDSAVVHYRAAMQGDSLLADPY
                     VRLGSFYVERGDESLGVPLLEEALRKRIADPDRAELSIALGQVAKRRGDLNEMAVALN
                     NSWALLRNMRTEDPSNSFPYYLLGEMFLVKGFLDSAIANLQTAQFVEERPYYLGKIAL
                     ALGKAYDLKKQRKEAVAAYQFGLNVPSIPFDRRRLERYLKKPFRE"
     gene            complement(2576..3433)
                     /locus_tag="AC16GM_08590"
     CDS             complement(2576..3433)
                     /locus_tag="AC16GM_08590"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR2923770.1"
                     /translation="MAAKQKLGAHMSIAGGVYHAFTHGKGAGCDTMQIFTKSSNQWRA
                     KSLGKEEIEEYLRLQEETGIEPVVAHTSYLINIASSDKALNQKSREALAIEVERCEAL
                     QIPFLVLHPGSHLGAGEEIGIKNIAGALDWVHVQTPRAKAKIALETTAGQGAHLGFKF
                     EQLRDMIDLTKEKERLAVCLDTCHVFAAGYDIRTEKGYEQTIADFDRTVGLDRLGAIH
                     FNDSKKGLGSRVDRHEHIGKGFLGKEAFGFFLNDKRLAHIPKILETPKGPDYKEDKIN
                     LAVLRKLIK"
     gene            complement(3544..4167)
                     /locus_tag="AC16GM_08595"
     CDS             complement(3544..4167)
                     /locus_tag="AC16GM_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR2923771.1"
                     /translation="MVIEPAHQTVSDIYKLMIGVIVPRPIAFVSTVTSEGQYNLAPFS
                     FFMAITSSPPTIAFSPIRRAGEKKDTLANVEATGEFVINVVNEDIAPQMCQASGDYPE
                     GVDEFKITGLTPIASSRVKSPRIKESPVSMECKVVKTVEFGKAPYQATLVAGEILLFH
                     IRDDLYRDGQIDLKALRAVGRLAGDWYCRVQDLFALKRPVIKRGKGA"
     gene            complement(4250..4534)
                     /locus_tag="AC16GM_08600"
     CDS             complement(4250..4534)
                     /locus_tag="AC16GM_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR2923772.1"
                     /translation="MAYVVVEPCIDVKDKSCILVCPVDCIYEGEKMVYIKPEECIDCG
                     LCVPECPVDAIFPEDEVPENFKPYIKINADYFTQKPAQEQPAQKSETPTA"
     gene            complement(4651..5304)
                     /locus_tag="AC16GM_08605"
     CDS             complement(4651..5304)
                     /locus_tag="AC16GM_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HIR2923773.1"
                     /translation="MEIFEIERFARDGVLEEESFRRALDQFDWTKYNGREVLIKGCAE
                     ILIPTWAYVVVAARLANHAKKVYYGEVQKPIPVYERGNGSRPAATPSTTITRPSGPVR
                     VTFQPANKVVTGQAGQTILEIATDNDIVLEYECGGNCQCTTCHVIIEQGLEQLSVKED
                     SEVDMLASAEGLTEKSRLGCQARVYGDIVAVIPERDPFKLDSLLKEMGVDPKDLPPL"
     gene            complement(5556..>6065)
                     /locus_tag="AC16GM_08610"
     CDS             complement(5556..>6065)
                     /locus_tag="AC16GM_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923774.1"
                     /translation="SHATGSPWTAHELEKIAKLNTSVRDVSWDYCRKVGVTKGTWTYS
                     DGTVERTVAVRTWFDDNAAGNRISEIMQSKGILPGAYTGEYEIPRAVEENLGGQIIDM
                     YMLKAINLVKKIQDLDGGLKRKYLLSVIHSDRTYYFLLKRMDVNPFIYTEQVYGSGVI
                     LDKGSKANR"
BASE COUNT         1337 a         1774 c         1534 g         1420 t
ORIGIN      
        1 gtgcgccagt gttttgcccc ccacggtaat ttcgcccagc gtcggaatca aaagtgttcc
       61 ctcaggggtc accaccagcg attgggtctg ctcaaaacta ccccacactc ccaaaaccag
      121 cacatcgccc ggtcccacca cataatcatt gggatcaata atctgatcga gcgaggctga
      181 cggtgcggct cccaatcctc cagacggtgg tggcacctgg ctatgcgcgg ataaactaac
      241 gacaacacag aagaaaaaga gtgcccgtct aaccactctc cgctcgcttg ccgtttgagg
      301 ccaaaggaaa ggggcgtgca tactgctaac aggagaagta taaaccaaaa tcgcagaaag
      361 tcaacttctg aatgggtcta ccgacagggc tccgatggcc atctccgtgc cggctggtct
      421 agcccctccg gtttcctgaa ccggtctcgg cgacgccggt caagccggat accactccac
      481 acccggcttg tgagcgagtg cttttccctc aattctcaat cctcagtcct caatcctcac
      541 caccccgcac ctcagagccg aggtatcacg cctcggagaa caaagcggaa ctcaaagtgc
      601 tcttactccc ggaacggctt cttcaggtaa cgttctaaac gccgccggtc aaaaggaatc
      661 gacggcacgt ttaacccgaa ctggtacgcc gccaccgctt ctttccgctg ctttttcaaa
      721 tcgtacgcct tccccagcgc cagtgcaatt ttgcccaagt agtagggacg ttcttcgaca
      781 aattgagccg tctggaggtt cgcgatggcg gagtcgagga acccctttac cagaaacatc
      841 tcgcccagta aatagtaagg gaacgagttg gaagggtctt cggtacgcat attgcgcaag
      901 agagcccagg agttgttcaa agccaccgcc atttcattca aatcgccccg gcgtttggcc
      961 acctgcccca gcgcaatgct cagttcggcc cggtctggat cggcaattct tttccgcaga
     1021 gcttcttcca gaagcggcac tcccagggat tcatctcccc gttcaacgta aaaagacccc
     1081 aaccgcacgt agggatcggc caggagactg tctccctgca tcgccgcccg gtaatgcacc
     1141 accgccgaat ccagctggtc ggcgaggagg aaaaaattgg ccgccacgac ctggttctca
     1201 gcggtcggat ggaaatggag attggttaaa gcgagcttga ggccctgcgg gtactgcccc
     1261 aggaggtagt gggtcagtac gagctggccg acctcatatt cgtcggtcga atccagcgcc
     1321 actaatttct ggaacagttc cagggcgtcc cggtagtggc cgaagcgcaa ggcccgctcg
     1381 gcaaagtggt tcaaatccgg tttgagcgcc gggaaggtat ccagataggc ccgccattcc
     1441 gcttccagcc cctccagcgg ttttccgtag accgccgtgc aggtgctgtc cagactcaag
     1501 tcggtcgcct gccggtagag ctgttccaag cgcgcccccc cgtaggtcgt cgcgaggtag
     1561 cgaacgaacg aagccgcctg gatcgtcgcc tccttggccg gctgagcata gtaatcctgg
     1621 cttttcgaca gcgtggccaa cttcagcagt cgccccgccc ggaggtcctg ttgcgcgtag
     1681 tagtggctgt aatccaagta ccccgccagc ccttcggcca aaagggccgg cgcatatccc
     1741 cagaggtggc atatcttttc taaaataacc ggaaaaggat cgaccccgct tttcccgggt
     1801 tggtgaacgg cacagacacg ataccgcaga ggatccaccg caaagggaaa gcgccggtcc
     1861 cacacaagcg ccttggtttg acagggaaag tagtaaaatc ttacccgctc cggcgtggtg
     1921 agatcaaacg ttcgctgcaa ggcatccagt tcgtcctcca gattatcgcg ccagcggttc
     1981 cactggaaat cgcccaacga ccagaggcgg tagtagaaga tgaaacgggg actcccttcg
     2041 tagttccaga aggtcgagtc gagcggactg gtgtcgcact cgaaagtcgt ctcggctggg
     2101 gagggaacca aagtccactg caaggtggag cgccctttga gtggtagcga cgccagggta
     2161 agcgtctcac cccaattcaa agtgtcaatg cgcaggaaat gttccggctg gcccccgaac
     2221 gtgaaaagtt ccaactccag cccaatcatc ttcttggaaa taggtgtgac ggtgagatcg
     2281 aagatgaaat tccccgccag gccgttcacc cggacgttgt aaggacagac aaattctcgc
     2341 cggtcaatca ctcccgcctg cggtggattg cccctgtagt tttcgactaa ctgactggaa
     2401 aaccgaaccg ctttttccgc gtgtcctgga acaaccataa tgggtaaaca caatagcagg
     2461 aacgcgagaa tactgtttgc ctttctgtag gagcgcacct gcacgtgtcc tcttggtccc
     2521 gagcacaatc gaggggagcc aagtcgcagg agatgcgccc gatccaagct ccgcatcatt
     2581 taatcaactt ccgcaatacc gccaagttga tcttgtcttc tttgtaatcc ggccctttgg
     2641 gcgtttccag aatcttggga atgtgggcca aacgcttatc gttcaagaaa aagccgaacg
     2701 cttctttccc caagaagcct ttgccaatgt gttcatgccg atccacccgg ctgcccaagc
     2761 ccttcttgga atcgttgaag tgtatcgcac ccaacctatc caggcccaca gtgcggtcga
     2821 aatcggcgat ggtctgttca tacccctttt ccgtccggat atcgtagccc gctgcaaaga
     2881 cgtggcaggt atccaggcag actgctagac gttccttctc tttggtaagg tcaatcatgt
     2941 cccgaagctg ttcgaactta aaccctaaat gcgccccctg accggcggtg gtttccaaag
     3001 cgatttttgc tttggccctt ggggtttgca catgcaccca gtccaacgcc ccggcgatgt
     3061 tcttgatgcc gatctcctcc cccgcgccta agtgcgagcc ggggtggagc acgagaaagg
     3121 gtatctggag cgcctcgcag cgctcgactt cgatagcgag cgcttcccgt gacttctgat
     3181 tcaaggcctt atccgatgag gcaatgttga tgaggtacga agtgtgggcg acgaccggct
     3241 cgatgccagt ttcctcctgc agccgcaagt actcttcgat ttcctctttt cccagagatt
     3301 ttgcgcgcca ctggttggaa ctcttggtga agatttgcat cgtgtcgcag ccggcgcctt
     3361 tcccgtgggt gaaggcgtgg tacacccccc cggcgatcga catgtgcgcc cctaatttct
     3421 gcttggccgc catcggtgct cctcggtgca ggtcacttta tcaatacgca ttctcgagaa
     3481 gtaacgcacg caggtaatga atggtcaaaa gaaaatctcc gtgtcctccg tggtgaaaag
     3541 gatttatgcg cctttacccc gtttgatcac cggccgtttc aacgcaaata aatcctgcac
     3601 ccggcagtac cagtcgccgg ccagccggcc tactgcgcgc agggctttga ggtcaatctg
     3661 gccgtcgcgg tacagatcgt cccggatgtg gaagaggaga atttcgcccg ccacgagggt
     3721 cgcctgatac ggtgctttcc cgaactccac ggttttgacc actttacatt ccatggagac
     3781 cggcgattct ttgatccggg gcgatttgac ccgggaactc gctatcgggg ttagcccggt
     3841 gatcttgaat tcatccacac cttccgggta atcaccagat gcctggcaca tttggggtgc
     3901 tatatcttcg ttgacgacat tgatgacgaa ctccccggtc gcctccacgt tggcgagcgt
     3961 gtccttcttt tctcccgccc ggcggatggg ggagaacgct atcgtcggcg gcgaggaggt
     4021 aatcgccatg aaaaacgaaa agggtgccag gttgtactgg ccttcggagg tgaccgtgga
     4081 aacgaacgcg atcggccggg gcacaatcac cccgatcatc aacttgtaga tgtcactgac
     4141 ggtctggtgg gcaggctcga tgaccacgag aaagttaccg aggattgagg agggactgta
     4201 accgcgctct cccgagcgtg gtgatttgga ttatggccaa aatgaacctt caggcagtcg
     4261 gagtttcaga tttctgtgcc ggctgctctt gagccggctt ttgggtgaag taatcggcgt
     4321 tgattttgat gtacggtttg aagttctccg gcacttcgtc ctcggggaag atggcgtcca
     4381 ccgggcactc cggcacgcac aggccgcagt caatgcactc ctccggcttg atgtagacca
     4441 tcttctcccc ttcgtagatg cagtcgaccg ggcaaaccag aatgcacgac ttgtctttga
     4501 catcgatgca cggctcaacg acaacgtacg ccatttacga gtatcctccg accatcttgc
     4561 gacggcacag catagcagtg attccgggaa aaatcaagcc gctctggttc cttttttcat
     4621 cactcgtaac caatcaccca tcactgtttt tcacagcggc ggcaaatctt tgggatcaac
     4681 ccccatctct ttaagcaggg aatcgagctt gaaagggtcc cgctccggaa tcaccgccac
     4741 gatgtccccg tagacacggg cctggcagcc cagccgcgat ttttcggtca acccctcggc
     4801 gctggcgagc atgtccactt ccgagtcctc tttcacggat agctgttcaa gtccctgttc
     4861 gatgataacg tggcaggtgg tgcactggca gttccccccg cattcatatt ctaagacaat
     4921 gtcattatcg gtggcgattt ccaaaattgt ctggcctgcc tggccggtga ctactttatt
     4981 ggccggttgg aacgtcacgc gcactggccc ggacgggcgg gtgatcgtgg tggaaggagt
     5041 ggcagcgggt ctggagccgt tccctcgttc ataaaccgga atcggttttt gcacctcgcc
     5101 gtagtaaacc tttttcgcgt gattggccag ccgcgcggcc acgacgacgt acgcccacgt
     5161 cggaatgaga atctcggcgc atcctttgat caacacctcc cggccgttgt acttcgtcca
     5221 gtcgaactga tctaaggcgc ggcggaacga ttcttcctcc agcaccccat ctctggcgaa
     5281 ccgttcaatt tcgaaaattt ccatttattc cttctggaag gccactccta ctcaaccgtt
     5341 atacctgtta gagtttccat gcctttgctt aatttctcat tcgccgttta attttttaca
     5401 atgctcttcg gcttcttctc ttgtaaggaa ccgtcctata acttccttcg tttctgtatc
     5461 gaaaactacc caaggcaggt accctctttt ctcgtaatcg tgccgcacca cgtattgcct
     5521 tgtaatcatt tttgctctcc ttttcttgac ctcaattacc tattcgcctt tgaacctttg
     5581 tctaaaataa ctcccgaacc gtaaacctgt tcagtataaa taaatggatt gacatccatc
     5641 cgtttcagaa gaaaataata tgtgcggtct gaatgaataa cagataaaag atattttcgc
     5701 ttcaaaccgc catccaagtc ctgaattttt ttaactaaat taatggcctt gagcatatac
     5761 atatctatta tttgacctcc cagattctct tctaccgcac ggggtatttc atactcaccg
     5821 gtatatgcac ccggcagtat acctttgctc tgcataattt cactaatgcg attgccagca
     5881 gcattatcat caaaccaagt tcggacagcg acggtacgtt caacggttcc atctgaatac
     5941 gtccaagtcc cttttgtcac gccaactttt ctacaataat cccaactaac atctcgaacc
     6001 gaagtattca gcttagctat tttctccaat tcgtgcgccg tccaaggact accggtagca
     6061 tgaga
//
LOCUS       DBNGZD010000319         4401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1926308, whole genome shotgun sequence.
ACCESSION   DBNGZD010000319 DBNGZD010000000
VERSION     DBNGZD010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1926308"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="AC16GM_08615"
     CDS             complement(<1..429)
                     /locus_tag="AC16GM_08615"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR2923775.1"
                     /translation="MFPLKDDIPSEKQPIVTIALIVANILAFILQLNSAYGFQTNMFR
                     MGLIPYEITHRVELNPEAPFPIYATLFTSMFMHGGFFHLGGNMLYLWIFGDNVEDQLG
                     HFRFLLFYLLSGIAAVSIFILTSPNSQTPLVGASGAIAGVL"
     gene            complement(573..1814)
                     /gene="icd"
                     /locus_tag="AC16GM_08620"
                     /pseudo
     CDS             complement(573..1814)
                     /gene="icd"
                     /locus_tag="AC16GM_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878150.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
     gene            complement(1898..2833)
                     /locus_tag="AC16GM_08625"
     CDS             complement(1898..2833)
                     /locus_tag="AC16GM_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR2923776.1"
                     /translation="MKRSGHIPWGKLRVGLLLLVALLLFLWAAFTGGFFKPLQHYYEL
                     RTAFTNVQGLVTGSPVWVNGLEVGQVKSIALAPHLQENQVEVTVRLKREVWPYLRTDS
                     KIRVGTIGLLGDKYIEIFAGSDTATKLPPGAFLPAEKPVDAQAMFAEATESLRRLTPL
                     LDDIAAMTRKMRSGRSSIGRLIDDQKLYDDLVEATGAITRVVKNIDRGQEQALTRMAN
                     AAASLDTLAKKLYRGEGSAGKLVTDTALYTSLAHVAGQADTLFGKIGRGEGALGKLTQ
                     EEQLYAEISKLVTELDRLVTDIKAHPKKYFKFSIF"
     gene            complement(2858..3652)
                     /locus_tag="AC16GM_08630"
     CDS             complement(2858..3652)
                     /locus_tag="AC16GM_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2923777.1"
                     /translation="MIAVERINYTIGDHQILRDVSLTVAAGETVVIIGPSGSGKSTLL
                     KIILGLAVPDSGRVFIAEQELTRARGQRLNDLRQQIGMVFQEGGLFDSLTVGENVGYY
                     LFEHTQMKEAEIEQVVRQTLATVGLDEDLIDAMPHTLSGGMQRRVAIARALAAHRPKV
                     MLYDEPTTGLDPISVEMVTECIAGLQQKLNLATVVVTHEIADALKIGSRFLVLNEGAV
                     VFQGNPDELARTDQPFVQQFLLPFRETLALAAEHFQVGPNNHGRNV"
     gene            complement(3649..>4401)
                     /locus_tag="AC16GM_08635"
     CDS             complement(3649..>4401)
                     /locus_tag="AC16GM_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR2923778.1"
                     /translation="KFLLGLQEIFFFAIRMFGALLRPPWYFRETYEQLYFIGVGSLFL
                     IVLTGIAAGQALALQTTKELADFGAKDYIGRIMTISVLRALGPVLTGLMVAARVASGI
                     TAEIGAMRSSNQIDALIAFGTDPIKKLAVPRLVSLLIMLPALTLITDAIAITGGFLIA
                     KFISHVSAHLYWANVKQKLTYGNFLVGIVKPFAFAIIIAAVACYKGFTARGGTKGVGL
                     ATMESVVISSITILVVDFLLTKVVFSFLGWSA"
BASE COUNT          915 a         1199 c         1339 g          948 t
ORIGIN      
        1 cagcacgccc gcaatggctc cggaagcccc taccagcgga gtctgggaat tcggcgaggt
       61 caaaatgaaa atggagacag cggcgatgcc acttaagagg tagaaaagta agaagcgaaa
      121 gtgacccagc tggtcttcca cattgtcccc gaagatccag aggtagagca tgttgccccc
      181 caggtggaag aagccgccgt gcatgaacat gctggtgaag agggtggcgt aaatcggaaa
      241 gggggcttcc gggttcagtt cgacccggtg cgtgatctca taggggatga gccccatccg
      301 gaacatattc gtctggaagc cgtaggcgct gttgagttgc aggatgaaag cgaggatgtt
      361 cgcaacgatg agggcgatag tgacaatcgg ctgcttttca ctcgggatgt cgtctttgag
      421 cgggaacata aggtcaggtt tccgattttt gtaagtgctg tgatggaaaa gtacaggaag
      481 gatggcaggg tgtcaatcat ttttggtgga gaaaagtgaa taactttcaa agcgacagag
      541 agaatttcga gaaaagaatt tttttcagtc tcctacatgt tcttaatgac ctccttcgca
      601 aactgcgagg tcgaaacctt ggtggcaccg tccatcaagc gggcgaggtc gtaggtcacc
      661 ttcttctgtt gaatcgtgcg ttcaatgcca gcggtgatcc tctgggcagc ctctttccag
      721 ccgaggtatt cgagcataaa gcaagccgag agaacgagcg agcagggatt gaccatatct
      781 ttgtcggtgt acttgggagc cgtgccgtgg gtggcttcga acaagccaag agaatcgccg
      841 atgttggctc ccggcgccat ccccaggccg cccacctggg ccgcgcaggc atcggagagg
      901 tagtcgccgt tctaattcgg tgtggccagg atatcgtatt cgtcggggcg cagcagtacc
      961 tgctggatca tcgagtcgga caggcggtca tttatcaaga gccggccctc gggccgccgg
     1021 ccgctgaact tttgctccac ctctacttca gtgacgatct gattccggaa ttctttttcg
     1081 gcgactgcgt agccccactg ggcaaaggcg ccttcggtgt atttcataat gttgcccttg
     1141 tgcaccaggg tcacgctctt cttttggttg gccagagcat actgaatggc tttgcgcacc
     1201 aggcgctggg ttccggttac cgaaatcggt ttgatgccga tgccggagtc ggcccggact
     1261 ttcactttca gctgtttatt caaaaaggaa atgatttttt tggcttcttt tgaacctttg
     1321 ggccattcga gcccggcgta gacgtcttcg gtgttctcgc ggaagatgac cacgttcatc
     1381 ttatccgggt gtttgaccgg ggcggggacg ccggggaacc agcgcacggg ccggatgcag
     1441 gcgtagaggt tcagtacctg tcgtaaagtt acgttcaaac tgcggaaacc gccgcctatg
     1501 ggcgtcgtga gcggcccctt caacgccaca atgtagtgtt tgatggccgc aatagtttct
     1561 ttgggtagcc actggccgaa gcggtcgcgg gctttttctc ccgcgaagat ttcgaaccag
     1621 acgaggtgtc ggttcccgcc gtaggttttc tcgacggctg catcgacgac cgttcgggtg
     1681 gcccgcatga tgtcgcggcc cacgccgtcg ccttcgatat agggaataac cgggtcgggc
     1741 ggaacaatca gttcattctt tttgacttcg atccggtgtc cggcagcggg cggagcgaga
     1801 gattcgaatt tcatggcggt aaaataagca aacggcgagt tggacgaaag tcgaatcaga
     1861 aaatccgata catttaatcc gcttgctcaa gcggcaatca aaagatgctg aacttaaagt
     1921 atttcttcgg atgggctttg atatcggtga ccagccggtc cagttcggtc actaatttgg
     1981 agatttcggc gtacagttgc tcttcctggg tcagttttcc cagagcgccc tctccccggc
     2041 caattttgcc gaaaagggtg tcggcctgcc cggccacgtg ggctaaactc gtgtacaggg
     2101 cggtgtcggt gacgagcttc cccgcactgc cttcaccgcg gtagagtttc ttggccaaag
     2161 tatcgagcga cgcggcggca ttggccatcc gggtgagcgc ctgttcctgg cctcgatcga
     2221 tgtttttgac cacgcgcgtg atggccccgg tggcttccac cagatcgtcg tacagtttct
     2281 ggtcatcgat cagccggcca atactgctgc ggccgctgcg catttttctg gtcatggccg
     2341 cgatatcgtc cagcagcggc gtgaggcggc gcaggctttc ggtggcctcg gcgaacatcg
     2401 cctgggcatc gaccggtttt tcggcaggca ggaaggcgcc cggcgggagc ttcgtcgcgg
     2461 tatcggaacc ggcgaaaatt tcaatgtact tgtcgcccag aagcccgatg gtgcccaccc
     2521 ggattttgga gtcggtgcgc agatacggcc agacttcgcg cttgagccgc accgtcacct
     2581 caacctggtt ttcctggagg tgcggtgcca gagcgatcga tttcacctgg cctacttcca
     2641 gcccgttcac ccagacgggc gagccggtca ccaggccctg cacgttggtg aaggcggttc
     2701 tcagttcata gtaatgctgc aggggcttga aaaaccctcc cgtgaaggcg gcccagagga
     2761 aaagcaggag ggcgaccaga agaagcaggc cgacccgcag tttaccccag ggaatgtggc
     2821 cggatcgttt catggtcttc gctgccgcgt cagttgctca aacgttccgt ccgtggttgt
     2881 tcggccccac ctggaagtgt tcggcggcca acgccagcgt ttcccggaag ggcaggagaa
     2941 attgctgcac gaagggctgg tcggtgcggg ccagttcgtc cggattaccc tgaaaaacca
     3001 cggcaccctc gttcagcacc agaaagcggc tgccgatttt caaagcatcg gcaatctcgt
     3061 gggtgacgac caccgtggcc agattcaatt tctgctggag accggcgatg cactcggtga
     3121 ccatttccac ggaaatcggg tctaaaccgg tggtgggctc gtcgtagagc atcaccttgg
     3181 gccggtgcgc cgccagcgcc cgggcaatgg ccacccgcct ttgcatcccg ccggaaaggg
     3241 tatggggcat ggcgtcgatc aagtcttcgt ccagccccac cgtggccagg gtttgccgca
     3301 ccacctgttc gatttcggct tctttcatct gcgtgtgctc aaagagataa tagccgacgt
     3361 tctcgccgac ggtcaaggag tcgaacagcc cgccttcctg gaaaaccatg ccgatctgct
     3421 ggcgcaagtc gttcaacctc tggccgcgag cgcgggtcag ttcctgttca gcgatgaaga
     3481 cccggccgga atcggggacg gccaggccca agataatttt cagaagcgtg ctcttgcccg
     3541 atcccgacgg cccgatgatc accacggttt cgcccgctgc caccgtcagg ctgacgtcgc
     3601 gcagaatctg gtgatcgccg atggtgtagt tgatgcgttc gactgcgatc atgcgctcca
     3661 tcccagaaag gagaagacca ccttcgttaa caggaaatcc accaccagaa tggtgatgga
     3721 actgatcacc accgactcca tggtcgccag gccgacgcct ttggtgcccc cgcgggcggt
     3781 aaagcccttg tagcaggcta ccgcggcgat gatgatggcg aaggcgaacg gtttgactat
     3841 gccgaccaag aaattgccgt aggtcagttt ctgcttgacg ttggcccagt agaggtgggc
     3901 cgaaacgtgg gatatgaatt tggcaatcaa aaagccgccg gtgatggcga tggcatcggt
     3961 gatcaaagtc agggccggca gcataatcaa aagactcacc aggcgcggga cggccaactt
     4021 tttgatcggg tcggtgccga aggcgatcaa agcgtcaatc tggttcgaag agcgcatggc
     4081 gccgatttcg gcggtgatgc ccgaagcgac ccgggcggcc accatcagtc cagtcagtac
     4141 cggtcctaac gcgcggagga ccgaaatggt cataatccgg ccgatgtagt ctttggcgcc
     4201 gaaatcggcc agttccttgg tcgtttgcag ggctaaggcc tgcccggcgg cgatcccggt
     4261 gagcacgatc aaaaagagcg agcccacccc gatgaagtac agctgctcat acgtctcacg
     4321 gaagtaccag ggcggacgga ggagtgcgcc aaacattcgg atagcaaaaa agaagatctc
     4381 ctggaggcca aggaggaatt t
//
LOCUS       DBNGZD010000320         4394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1857790, whole genome shotgun sequence.
ACCESSION   DBNGZD010000320 DBNGZD010000000
VERSION     DBNGZD010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1857790"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="AC16GM_08640"
     CDS             complement(<1..1401)
                     /locus_tag="AC16GM_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR2923779.1"
                     /translation="MPCENELKEALGDYLKGMLPDPKTAMVREHLKQCPDCRSDLDQL
                     QFLLPGLAGLSEEHFPVETLADYAFRVKTAARQTETLNWGEEKIEEHLPVCDRCQAEL
                     DKLTAFEKEFQRVSKKSVTEPSLEITPLSRWSLFRSRKVWYAAAAAAALILLLLPTLP
                     LRSQGPMIAVLPFEYTGPAEKEYYVFGVTDEITARLGKVSAIGVIAPSSTMQYKNTAQ
                     KPRQIGEELGVLYLLTGSVRWDTTGKIAKLQVVPKLVRTRDQKQLWTEPFEIIRGELC
                     NLTGVIARKVIAALNVEFLESERQAMEACPTGNIEAYEYYLRGLEYAKRYEKAGIAIQ
                     MYEKAVELDPGFALAYAQLSFCHTFVYWFGDPTEERLSKIKETMERAFQLQPNLPEAH
                     LAMAWYVSMADLDIERILKECALAVKELPNNVDALYITGVMQRCAGRYEVAVDYFKKA
                     ARLDPLSPNISVELAHT"
     gene            complement(1440..1991)
                     /locus_tag="AC16GM_08645"
     CDS             complement(1440..1991)
                     /locus_tag="AC16GM_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2923780.1"
                     /translation="MEKPDEELAITQFTQGVPNNYIDGLIRQTVRFQIWGKKFEAEEV
                     IQNTRLALLSCFREGKYKGKGLTTYVCRIAAIQSVMEMRRHYRSEKYRAEWSEQANQQ
                     PGPAGNPLTTLLEREKKDRAIRVLKTLGKLCRQLLMLKFYKGLSYREIGGRLGLTEVN
                     VRVSVHRCLQKSKEIAKKMEEGQ"
     gene            2340..3947
                     /locus_tag="AC16GM_08650"
     CDS             2340..3947
                     /locus_tag="AC16GM_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923781.1"
                     /translation="MPPRLPAVAGGDVYVQDGGETSIGINYDDGNNLVAGYNTWPWNL
                     DSHSNSTDGNLTWTSRTFPNGAGIFTGWTADPWTNAGNAAGEFFSTRLKVTSGFPPPP
                     PFNFDSVHCIVARSTDGGASFSLFFEKFKAIFQDKEAVDIDKTTARGGGAGITHDGKV
                     YLGYDDDGLAPNFSYRGSFLQVISSAGVADTEIQISGTGSPPFRGRYLQPVAGITDGT
                     VYVKGHNFGSTIPAIFHEITNGGAGPNTFSKSSFSYFPAGQALGGSGFWGVNGHRIPT
                     NIFGYLDIDRSSGPRRGYLYFITNRNPNRANPALDQGDVYLSVSTNGAASWSSALIPT
                     AAGKTQYTPMLDVDEQGWIHIAYYQNETGSVNGGVLNASTANVYYTRSTDGGTSWAVP
                     VQVNEPANALDYEDPPPNRSGSGYYLIGDYAQLQATGSGPSTKVYILWTGYDKDRSDA
                     FVGDAKARVWCTTIQCFPRGDVTSDAVTDIVDIVALIQNVVFGDPLPNETAGDVNCDN
                     VRDIVDIVQLIGVVALGQPTPCCFGPR"
BASE COUNT          929 a         1209 c         1210 g         1046 t
ORIGIN      
        1 ggtgtgggca agctccacac tgatgttggg agaaaggggg tcaagccggg cggccttctt
       61 gaagtagtca accgccactt catatctgcc cgcacacctt tgcataactc cggtgatata
      121 gagggcgtct acattgttgg gaagttcctt tacagcaagg gcgcactctt ttaggatgcg
      181 ctcaatatcc aggtcggcca tgctgacata ccaggccatt gccaagtggg cctcaggcaa
      241 gttgggctga agctggaacg ctctttccat cgtctctttt attttggaaa gccgctcctc
      301 ggtggggtcc ccgaaccaat acacaaaggt gtgacaaaac gaaagctggg cataggccaa
      361 agcaaagccc gggtccagct ccaccgcctt ctcatacatc tgtatggcta tgcccgcttt
      421 ctcatatcgc ttggcgtact ccagcccgcg caggtaatat tcatacgctt cgatgttgcc
      481 ggtgggacaa gcctccatcg cttggcgctc cgattccaaa aattcgacat tcaaagcagc
      541 aatcaccttc cgggcaatta cgccagtgag attgcagagc tcccctctaa tgatttcaaa
      601 cggttccgtc cagagctgct tttggtctct ggttcgcacc agtttgggaa cgacctgaag
      661 tttggcgatt ttcccggtgg tgtcccagcg aacagaaccg gtcaaaagat aaaggacccc
      721 aagttcctcg ccgatttggc gcggtttttg ggccgtgttt ttgtattgca tcgtgctgct
      781 cggagcgatg accccgatgg cgctcacttt gcctaaccgg gcagtaatct cgtcggtgac
      841 tccaaagaca taatattcct tttcggccgg gcctgtatat tcaaacggca agaccgctat
      901 cattgggccc tgggagcgga gcgggagagt gggtaaaaga aggagaatca aggccgccgc
      961 ggcggccgcg gcataccaga ctttgcgaga acggaaaaga gaccacctgc tcaaaggagt
     1021 tatttcaagg gaaggttcag ttacgctctt tttggaaacc ctctgaaatt ctttttcgaa
     1081 agcggtcagc ttatccagtt cggcttggca ccggtcgcaa acgggcaagt gttcttcaat
     1141 tttttcctct ccccagttta aagtttccgt ttgccgtgca gccgttttga cacggaaggc
     1201 gtaatccgcc aaggtttcga ccggaaaatg ctcttcggac aacccggcca ggccgggaag
     1261 caggaattgg agttggtcca agtccgaccg gcagtccggg cattgcttca gatgctcccg
     1321 aaccattgcg gtttttgggt cagggagcat tcccttcagg taatccccca aggcctcctt
     1381 tagttcattt tcgcaaggca tacagcacct ttgttattgt tgcacagata gggaaagtgt
     1441 tactggcctt cttccatttt cttggcgatc tctttgcttt tttgcaggca gcgatgaacg
     1501 gaaaccctga cattaacctc cgttaggccc aagcgcccgc ctatttccct gtaggacagt
     1561 cccttataaa atttgagcat cagcagttga cggcacaatt tgcccagagt ttttagaact
     1621 cgtatcgctc tatctttttt ttcgcgttcc agaagggtcg ttaaggggtt tccggccggg
     1681 cccggctgct ggttggcctg ttcggaccat tcggcccggt atttttcgga gcggtagtgg
     1741 cgtctcatct ccattaccga ttgtatggcg gcgatgcggc acacgtaggt tgtcaaccct
     1801 ttacccttgt atttcccttc tctaaaacag cttaagaggg ccagccgggt attctggatg
     1861 acctcttcgg cctcaaactt ttttccccag atttggaaac ggacggtttg ccggataagc
     1921 ccgtctatat agttattggg caccccttgg gtgaattgag ttatagctaa ctcttcgtcg
     1981 ggtttctcca taagctttgg gtcaatatag caccgtaatt gggtttttct gtaacaaaag
     2041 caaccggtcg gctactatat aagcgaagac tgaaactttt caatcggagg tgtggtatga
     2101 accggcaaag acttttttgg gtagccgttg ccgggcttct ggctttcgca attgaagtcc
     2161 cttgcgccag tgcccagcaa cttctcaaaa ccaccttttt cccggagcct gcaacgcctc
     2221 cgccgacaga ggtaaagcca ggcccgccac ttttcttggg caagctgttt gttcaggaca
     2281 gcgcggggaa ctgggtaccc atcaaaccgc gtgcaccggt gttccggccg ggggaggtgg
     2341 tgcctcctcg cttaccggcg gttgcaggtg gcgatgtcta cgttcaggac ggcggggaaa
     2401 cctccatagg gatcaattac gacgatggaa ataatctcgt cgctggatac aatacgtggc
     2461 cgtggaacct tgattctcac agcaactcca ccgacggcaa cctcacctgg acctcccgca
     2521 cctttcccaa tggcgccgga atcttcaccg gttggaccgc tgatccctgg accaatgccg
     2581 ggaacgcagc cggagaattt ttctccacac ggcttaaggt gaccagcggt tttcctcctc
     2641 ctcccccctt taacttcgac agtgttcact gcatcgtggc ccgttcgacc gacggcggcg
     2701 ccagtttttc gcttttcttt gagaagttta aggccatatt tcaggataaa gaggcggtgg
     2761 acatagacaa gaccacggcc cggggagggg gagccgggat aacgcacgat ggaaaggtat
     2821 atctcggtta tgacgatgat ggtcttgcac ccaactttag ctacaggggg tccttcctgc
     2881 aagtcatctc ctccgccggg gtggccgata cggaaatcca gatttccgga actggcagcc
     2941 cccctttccg aggccgctac ctgcagccgg tggccggcat taccgacggc accgtgtatg
     3001 ttaaggggca caactttggc agcactatcc cggccatttt tcacgaaatc accaacgggg
     3061 gagcaggtcc gaacaccttc agcaagtcgt ctttctccta ttttccggcg ggtcaggcac
     3121 ttggtggaag cggcttttgg ggcgtaaatg gacatcgaat acccaccaat attttcggct
     3181 atcttgacat tgaccggtcg tccgggccgc ggcggggcta cctctatttc atcacgaacc
     3241 gcaaccccaa ccgcgccaac cctgcgctgg atcaagggga tgtgtatctt tcggtctcta
     3301 ccaatggagc cgcgagctgg tcctccgcgc tcattcctac ggccgcgggg aagactcaat
     3361 acacgcccat gctggatgtg gatgagcagg gctggattca catcgcctac taccagaatg
     3421 aaaccggctc tgtgaatggc ggagtgctga acgccagcac ggcgaacgtc tactacactc
     3481 gctcaaccga tgggggcacc agttgggcag tgccggtgca ggtcaacgaa ccggccaacg
     3541 ctttagacta tgaagacccg cccccgaacc gaagcggatc cggttattac ctcattggcg
     3601 actacgccca actgcaggcc accggctccg ggccgtccac caaggtctac atcctctgga
     3661 caggatatga caaggatcgc agcgacgctt tcgttggtga tgccaaagcg cgggtctggt
     3721 gtacgacgat acagtgcttc ccgcgcgggg atgtcactag cgacgctgtg accgatattg
     3781 tggacattgt ggcgttgatt cagaacgtcg tgttcgggga tcccctgcca aacgaaactg
     3841 cgggggatgt gaattgtgat aacgtcaggg acatcgtgga tattgtgcag ttgattgggg
     3901 tggtggcgtt ggggcaacct accccgtgct gttttgggcc gaggtaactc aaaccgagag
     3961 ctgacttatt gctgaggaca ttgccttccg cgcgcaccgg aaggcgattg cggagttttt
     4021 ccagtccgcc tgaggcggac tcggggttaa ccgcctgtgc gagttgccct gaggcgacgc
     4081 ccgtacgggc gccgaggata gaatcctcgg atacgactcg tctctcttcg ggaggcgtcg
     4141 ggctcggaga cgagcccgac taccttatcc ctgacagcgg tttctccgga agcgtgcccc
     4201 gcggatggtt ggtggccggg ggatacagga gtaaatctat ccctttaaga taagaaaatc
     4261 cttgacaagt tgacgccgct gaattatact ctcccccgct gaggagggag ggctaaagcg
     4321 gcagacccca ctcccgccgt tggcgggaca ctgctttagt cggcacaggt taaaaaagga
     4381 aatgtagcgt agcc
//
LOCUS       DBNGZD010000321         4554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1718917, whole genome shotgun sequence.
ACCESSION   DBNGZD010000321 DBNGZD010000000
VERSION     DBNGZD010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1718917"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC16GM_08655"
     CDS             <1..366
                     /locus_tag="AC16GM_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923782.1"
                     /translation="ERLNAVVEGNQRKIGQATEDFASAAGRLKTLLETNAPKIDSTVT
                     SAREAASRMNEITTGLDSLSHSLHSFIDKIDSQQGTLGKLVNDDRLYGEIRKAANDLD
                     SLVLDIKKNPKKYLRIQIF"
     gene            541..2028
                     /locus_tag="AC16GM_08660"
     CDS             541..2028
                     /locus_tag="AC16GM_08660"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2923783.1"
                     /translation="MKRETNPIRISVVIPALNEVQLLPHCLRSLKNQNFGHSYEIIVV
                     DNNSEDRTAAVAAGMGARVVFEPHRGITWARQKGLEAARGEIIAWVDADTVVTVDWLQ
                     QIYAALNDADGVVAVSGKILYTKSTHWKGKLPGYWVAAVRYGDRAIRFLLRKPGTLWG
                     GNFAVQKKALLQVGGFNKNIEFYGEDTELSLRLGKAGKIQFNKNQIAYTSPRRFENQP
                     LIRTAWFYVSNFVRIMVFHRYAASRPKPGFPALKIYFQKVLPVFGMLSILVAFGFFAF
                     NPASQFYGRVYSAGLDWRQKVIALSFDDGPDEPYTSQVLNILDENGIKATFFIIGKNA
                     EYYPEAVREIVDRGHIIGNHSYSHYYLLPFENNDRIQKDLIKTENLLFHLTGLRTALF
                     RPPHGLRTPWFLRDVRRLHYSVITWSDMTDDYDSDESPQDIAKAILKHARPGGIIDLH
                     DGKNLGHGVDRSNTVKALPIIIDQLKKEGYTFVILPELLHINAYK"
     gene            complement(2143..2793)
                     /locus_tag="AC16GM_08665"
     CDS             complement(2143..2793)
                     /locus_tag="AC16GM_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923784.1"
                     /translation="MKNLRQLFNRRFIDVKLPLWLRVTLIAIFSLGIIGALAYLFSYA
                     RVGEAATLLVLTLTLIAVAMYVYYNYLVAYDIWAPLSFFYLEQDKDDPYQVLIILRNE
                     SKQTIRLFVDLRAMIYNSQSLYVDIDGRYAPTHVWELEPLSQAKGIIKITDILKKANF
                     TTSSMEKAASIQKEQNSQLRFSLKVKYLGLRANVGGYYPAQNYYFDFTRKELVLNV"
     gene            complement(2860..3063)
                     /locus_tag="AC16GM_08670"
     CDS             complement(2860..3063)
                     /locus_tag="AC16GM_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923785.1"
                     /translation="MLSFDLFKDETFPMVEPVRAKSASGGLVGVNGETEISTHARIMA
                     EEQLECKLKMSLSKLGYGSDLLS"
     gene            3078..>4554
                     /locus_tag="AC16GM_08675"
     CDS             3078..>4554
                     /locus_tag="AC16GM_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HIR2923786.1"
                     /translation="MSISFCFGIHSHQPVGNFDFVFEEAYEKAYLPFLKLLERYPAFR
                     TTLHYSGSLLEWLEKHKPEFFELLWALVKRGQVELLTGGMYEPILPAIPERDKIGQIR
                     KLTAYLNKHFGVKPTGAWITERVWEPHLPKALAQAGVEYVILDDTHFKYAGLTDDQLH
                     GYYVTDEEGYSVKVFPISKKLRYAIPFQPVEETIEYLRAVSVKGGGTPPLHCYADDGE
                     KFGGWPGTYKHCYESDWLEKFFAAITQNSDWIHLLTYGEAAKELVPLGKIYLPTASYA
                     EMLQWALPAEAGEKFEAFEKQLKETGLSEDYGQFVRGGHWRNFLAKYPEANQIHKKML
                     RVREKIERAEQKKGVSRKKIDSAWTHLYRAQDNDPYWHGVFGGLYLPHLRSAAYENLL
                     KAEAVAESTVHGQKVWLNIEETDFDLDGQPEILVETPQLNLAFSPHKGGTLFEMDYKP
                     KSVNFLDTLTRRKEAYHSRIDASTPSPNNPGAVATIHERIET"
BASE COUNT         1162 a         1097 c         1201 g         1094 t
ORIGIN      
        1 gagcggttga atgctgtggt ggagggcaat caaaggaaaa taggtcaggc gacggaagat
       61 tttgccagcg cggcggggcg gctgaagacc cttttggaga ccaatgcgcc caagattgac
      121 tccaccgtga cttccgcccg ggaggcggcc tcccggatga atgagattac taccggcctg
      181 gattcgctct cccattcact ccattctttt atagacaaaa ttgattccca gcaggggact
      241 ttgggcaagt tggtgaacga cgaccggctg tacggcgaaa tacgcaaagc ggccaacgat
      301 ctcgacagtc tcgtgctgga tattaagaaa aaccccaaga agtatttacg aattcagatc
      361 ttttaggtaa tcgctctgct ctccggtcgg aatttctctt ggccgttccc ggcgttaaga
      421 tttttttgat tcaaagggaa ggcgggacgt accaccgatt aatggctcta ttgccttccg
      481 ggtgcggccg gttctcttgg ttgcctttgc ggcagcgggc gcacaggggg taagaggcgg
      541 atgaaaaggg aaacaaaccc cataagaatt tccgttgtca ttccggcgct caatgaagtg
      601 caattactgc cccattgtct gcgcagtcta aaaaatcaga attttggcca ctcgtacgaa
      661 atcatcgttg ttgacaacaa cagcgaagac agaaccgccg cggtggctgc cggcatggga
      721 gcccgggttg tgtttgaacc gcatcggggc attacctggg cccggcagaa aggcctggaa
      781 gcggctcgcg gagaaattat tgcctgggtg gatgccgata cggtagtcac cgtagactgg
      841 ctgcagcaaa tttatgccgc cttaaatgat gccgacgggg ttgttgccgt aagcggaaaa
      901 attctgtaca cgaaaagcac tcactggaag ggaaagttac ccggctactg ggtggcggct
      961 gtccggtacg gtgaccgggc tattcgattt ttgctgagaa agcccgggac gctgtggggg
     1021 gggaattttg cagttcagaa aaaagcgctg ctccaggtcg ggggattcaa taagaacatt
     1081 gaattttatg gagaagatac ggagctgtct ttacgcttgg gcaaggcggg aaaaattcaa
     1141 ttcaataaga accagatcgc ctatacttca ccccggagat ttgaaaatca accgctcatc
     1201 cggaccgcgt ggttttacgt atccaacttc gttcggataa tggtctttca tagatatgcc
     1261 gcgagccggc ccaagccggg atttcccgcc ttgaaaatat actttcagaa agttctcccg
     1321 gttttcggca tgctctcgat ccttgtcgcc ttcggtttct ttgctttcaa tccggcttca
     1381 caattttacg ggcgggtcta ttccgcgggg ttagactggc gacaaaaagt catcgccctg
     1441 tcttttgacg acggcccgga tgaaccctat acctcgcagg tgcttaacat tttggatgaa
     1501 aatggcatta aggccacgtt cttcataatc gggaagaacg ccgaatatta cccggaggcc
     1561 gtaagagaaa tcgtcgaccg gggccacatt atcggcaacc attcgtacag ccattattac
     1621 ctgctgccgt tcgagaacaa tgaccgaatt caaaaagacc tcatcaaaac cgaaaatctg
     1681 ctttttcatt tgaccggact gcggaccgcc cttttccggc cgccccacgg tctacgcact
     1741 ccctggttcc tcagagacgt tcggcggtta cactacagcg tcattacctg gagtgatatg
     1801 accgatgatt atgattctga cgagtcgcca caggatatcg ccaaagccat tctcaaacat
     1861 gcccgaccgg gcggtattat cgatctgcat gacggaaaga atttaggtca cggcgtcgac
     1921 aggagtaata ccgtcaaagc gctgccgatt atcatcgacc aactaaaaaa agaagggtat
     1981 acgtttgtca tcctcccaga attactacac attaacgctt ataagtaatt tccccgcagc
     2041 gaatgacctg gatagtctgg tgctggatat aaagaagaat ccgaagaagt atttgaggat
     2101 tcagatttgt tagaaaaact taaaaggcaa ttattcggcg gattagacat tgagaaccaa
     2161 ttcttttctt gtaaagtcaa aatagtaatt ttgagcggga taatagccac ccacattagc
     2221 ccgaagaccc agatatttaa ccttaagcga aaaacgtaat tgactgtttt gctctttttg
     2281 tatactagct gctttttcca tgctgctggt agtaaaatta gcctttttga ggatgtcagt
     2341 aattttgata ataccctttg cttggcttaa aggctcgagt tcccatacgt gggtcggcgc
     2401 gtaacgtcca tctatgtcta catagagaga ttgactgtta tagatcatgg ctcttagatc
     2461 gacgaacaat cgaatggtct gtttgctttc atttcgcagg ataattaaaa cttgatacgg
     2521 gtcatcttta tcttgctcta agtagaagaa actaagaggg gcccagatat cataagcaac
     2581 gaggtagttg taataaacat acattgcgac cgcaataaga gtgagtgtaa gcaccaaaag
     2641 ggtggcagcc tccccgaccc gagcataact aaataaataa gccaaggctc caataattcc
     2701 aagcgaaaaa atggcgatta aagtgacacg gagccaaagt ggaagtttga cgtctatgaa
     2761 tcgccgatta aagagttgcc gtaagttttt catatcctaa gagccatatc tcttacatct
     2821 atatttttgc tgagccaata taacatcaaa tccatttatt tatgaaagga ggtctgaacc
     2881 gtatcccaat ttggaaaggg acattttcaa tttgcactcg agttgctctt cagccattat
     2941 tctggcatga gtgcttattt ctgtttcgcc gttgactccc accagtccgc ctgaggcgga
     3001 tttcgccctt acgggctcaa ccatagggaa ggtctcatct ttgaaaaggt cgaaggaaag
     3061 catactttcc ttcctttatg agtatatcct tctgttttgg cattcacagt catcaacccg
     3121 tcggcaattt cgattttgtc tttgaagagg cctacgagaa ggcgtacctg ccctttctca
     3181 aactcttaga gcgctacccc gcttttcgca ccacgctcca ctactccggc tcgcttttgg
     3241 agtggctgga aaagcataaa ccggaattct ttgaactgct ctgggcgctg gtgaagcggg
     3301 ggcaggtgga gcttttgacc ggggggatgt acgagccgat tctgccggcg ataccggagc
     3361 gggataaaat cggccagatc aggaaactta cggcttacct gaacaaacat ttcggcgtca
     3421 agccaaccgg cgcctggatt acggagcggg tgtgggagcc gcatttgccc aaggcgctgg
     3481 cccaggcggg ggtagagtac gtcatccttg atgacacgca cttcaagtac gcgggtctga
     3541 ccgatgacca gttgcacggg tattacgtga ccgacgagga ggggtattcg gtcaaggttt
     3601 ttccgatcag caagaaattg cggtatgcca ttccgttcca gccggtggag gagacgatag
     3661 aatatttgcg ggcggtgtcg gttaagggcg gggggacccc gcccctacac tgttatgccg
     3721 atgacgggga aaagtttggc ggctggccgg ggacgtacaa gcactgctac gaatctgatt
     3781 ggctcgaaaa attcttcgcg gcaattacac agaatagcga ttggattcac cttctcactt
     3841 acggcgaggc ggcgaaggaa ctcgtgcctc tgggcaaaat ctatctgccg acggcctcct
     3901 acgcggagat gctccagtgg gcgttgcccg ccgaggcggg agaaaagttt gaggcatttg
     3961 aaaagcaact gaaagagacg ggtctttccg aggattacgg ccagttcgtc cggggcgggc
     4021 actggcggaa ctttttggcc aagtacccgg aagcgaacca aattcacaag aagatgctcc
     4081 gggtacggga gaagattgag cgggcggagc aaaagaaagg cgtctcgcgc aagaaaatag
     4141 attctgcctg gacgcacctc taccgggcgc aggataacga cccttactgg cacggggtct
     4201 tcggcggctt gtacctcccc cacctgcgga gcgctgcgta tgagaatttg ctcaaagccg
     4261 aggcggtggc tgagtcgacg gttcacggcc aaaaagtatg gttgaatatt gaagagacag
     4321 attttgatct cgacgggcag ccggagattc tggtcgaaac gccgcagttg aacctcgcat
     4381 tttcgcccca caagggcggg acgttgtttg aaatggatta caagccaaaa tccgtcaatt
     4441 tcctcgacac cctgacccgg cggaaggaag cgtatcacag ccggattgat gcgtcaacgc
     4501 cctcccccaa caatccgggt gcggtggcaa ccattcacga gcgcatcgaa acca
//
LOCUS       DBNGZD010000322         2055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1301691, whole genome shotgun sequence.
ACCESSION   DBNGZD010000322 DBNGZD010000000
VERSION     DBNGZD010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1301691"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="AC16GM_08680"
     CDS             complement(<1..1816)
                     /locus_tag="AC16GM_08680"
                     /inference="COORDINATES: protein motif:HMM:NF046853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2923787.1"
                     /translation="MSNTLIEHRFYMNPPAGNQLWFLVYEGAAQVGTYNLVSASDVTP
                     AGPGEGWYSSGSINVPLVAGRFYLIVASFEQASNYFNEQGISPYPIPASFGELTAGAG
                     WDWAPTSNFPPDPVQNVPAFAFGSPVAYYQALVTGVGTSWLSANPDSGTVPAGESLDV
                     AVTFDATGLNGGDYYADVLINSNDPDRPQDTVFAHLHVTGAPDIAVSDTALDFGSIFT
                     GTAKTETLVVTNPGTDSLAINNITSDNAHFTVNITAFFLNPGDSQNVLVTFATNDTGL
                     FTGNLTITSNDPDEPTVNVSLRGRGLLPPDILVTPDSLAADLFTGEIAVETLTVANTG
                     YSDLNFTISIKDTGATAQAVKVRPTGISRLSGGSGALTGTKADLSPFQREISADVLAQ
                     TGSPGSVPKRSPGKISSSALQATTLLLTTTSVTGSVGQALTNLGVAYDLLQTDVFTGI
                     DFSPYTTIVVAMDGGLVEEPDVQALATAAASGKKLIMIGGTNYPPYYNGMQLYLLSHT
                     NQQGWTISSPPHLIVTNPSHPLAAGLPSTYTFLNSSAAFYMLRVSDGLAAVATNNGDG
                     HPSLLNKEIGSGMLIYFTSSPFDGYWSNPPDFAILQQVI"
     gene            complement(1801..>2055)
                     /locus_tag="AC16GM_08685"
     CDS             complement(1801..>2055)
                     /locus_tag="AC16GM_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923788.1"
                     /translation="GEADANGDGIVTADELAEYVHRNVREATNARQNPTSDRGSFDPN
                     NREALWRKPPELKYLEARRTLSLPDPEPGATSSPARCPTP"
BASE COUNT          428 a          558 c          655 g          414 t
ORIGIN      
        1 cgatcacctg ttgcaggatc gcaaagtcag ggggattgct ccagtaaccg tcaaacggcg
       61 agctggtgaa gtagatcagc ataccactgc cgatctcttt gttcaaaagc gacggatgac
      121 cgtccccatt atttgtggca accgcagcca gaccgtcact gacccggagc atgtagaagg
      181 cggcacttga gttaaggaac gtataggtgg acggcaagcc ggccgccagg gggtgggacg
      241 gattggtaac gatgagatga ggtggggacg aaatagtcca cccctgctgg ttagtatgag
      301 acaatagata cagctgcatg ccgttgtagt aggggggata gttcgtcccg ccgatcatga
      361 tcagcttttt cccgctggca gcggcagtgg ccagggcctg gacatcaggt tcctcgacca
      421 gaccgccgtc catagcgacg acaatcgtcg tgtagggtga aaagtcaatc ccggtgaaaa
      481 cgtcggtctg gagcaaatcg taggcgacgc ccaaattggt cagcgcctgc cccaccgatc
      541 cagtgaccga cgttgtcgtc aagagcagag tcgtcgcctg aagcgccgaa cttgaaattt
      601 tgcccggcga tcttttcggc acgctcccgg gcgaaccggt ctgcgccagc acgtcggcgg
      661 agatctcccg ctggaacggg gacaaatcgg ctttcgtccc ggtcagtgct ccgcttcctc
      721 cggagagccg agaaatgccg gtgggtctga ccttcaccgc ctgtgccgtc gcgccggtat
      781 ctttgatgga aatagtgaag ttcaaatcgc tgtaaccggt gttggcaacg gtgagcgttt
      841 caaccgcgat ttcgccggtg aagaggtcgg cggccagcga atcgggcgtc accagtatgt
      901 ccggcgggag taggccccgg ccgcgcaagg agacgttaac cgtcggctca tccgggtcgt
      961 tgctcgtaat ggtgagattg ccggtgaaaa gccccgtgtc gttggtcgca aaggtgacca
     1021 gaacgttctg gctatccccg ggattcaaga agaacgcggt gatattgacc gtgaaatgcg
     1081 cgttatccga ggtaatgtta ttgatggcca aactgtcggt tcccggattg gtaaccacaa
     1141 gcgtttcggt tttggcggtt ccggtgaaga tggagccgaa gtccaaggca gtatcggaga
     1201 cggcgatatc gggcgcgccg gtgacgtgga ggtgggcgaa aaccgtgtcc tgcggcctgt
     1261 ccggatcgtt gctgttgatg aggacgtcgg cgtagtagtc gccgccgttt aagccggtgg
     1321 cgtcaaaagt caccgccaca tccagagact cgccagccgg aacggtgccg gaatcgggat
     1381 tggccgatag ccaggaggtt cctaccccgg tcacaagggc ctgatagtac gccaccggag
     1441 acccgaaagc gaaagccgga acgttttgca ctggatcggg cgggaagttc gacgtgggtg
     1501 cccagtccca gccggccccc gcggttaatt cgccgaaaga ggcgggaatg gggtaggggg
     1561 agatgccctg ctcattaaag tagttgctcg cctgttcgaa agatgcgacg atgaggtaga
     1621 atcttccagc caccaaaggc acgttgattg aaccagaact ataccacccc tcacccgggc
     1681 cggcgggagt gacgtcggat gcgctgacta aattgtacgt tcccacctgg gcggcgcctt
     1741 cgtacaccaa aaaccagagt tgattgcccg ccggcggatt catgtaaaac cggtgttcga
     1801 tcaaggtgtt ggacaccgtg caggtgaaga ggttgccccg ggttcggggt ccggcaaaga
     1861 aagagttcgc cgggcttcca aatatttcaa ctccggcggc tttcgccaga gcgcctctct
     1921 gttgttcgga tcgaagctgc ctcggtccga cgtcgggttt tggcgggcgt tggtcgcctc
     1981 gcgcacattg cgatggacat attccgccag ctcatccgcc gtgacgatac catcgccgtt
     2041 ggcgtcggct tcgcc
//
LOCUS       DBNGZD010000323         3974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_608401, whole genome shotgun sequence.
ACCESSION   DBNGZD010000323 DBNGZD010000000
VERSION     DBNGZD010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_608401"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="AC16GM_08690"
     CDS             <1..628
                     /locus_tag="AC16GM_08690"
                     /inference="COORDINATES: protein motif:HMM:NF020660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HIR2923789.1"
                     /translation="AGFIDHFLGPGCTLEDFARARYPEEGDFVNQPYEAVFKTKGQKS
                     TVTFKRDGKVWVGSKQIPLRVEKELTFEARRSGFDVSYRLSNLSDQAVELWFGMELNL
                     GLLAPNAPDRYVLINSQRPAEVNFAATGSYVAVRTVALVDEWRKVQIELGLKEPVVLW
                     RFPIYTVSLSEGGMEKVFQAASFLFHRRLTLAPQEQRVWLLAVTLSSL"
     gene            763..1431
                     /locus_tag="AC16GM_08695"
     CDS             763..1431
                     /locus_tag="AC16GM_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923790.1"
                     /translation="MRRQLFGLIGSFLLLTPVIHAQDIDGKFGLRIHLGAWKSIQTDH
                     SDIWTIGPLGRFGIDYAVADFFTIGVAGGYRLVYEANLVGRTKGAGFTFDKLSGGNTF
                     RNQLYELTFGLRPLPHAKIAPYFANGVGFNFWQVKRTGGTLDSLTDRNGNSFKAQDKE
                     LTLSTGLGVELFPTENLAIDLGGRYHYRTKAFSDLKGAKDVWDAAFEDLPRGLFETYI
                     GLVV"
     gene            1513..2433
                     /locus_tag="AC16GM_08700"
     CDS             1513..2433
                     /locus_tag="AC16GM_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR2923791.1"
                     /translation="MDALGCSIDSDGDGVCDGVDQCPSTPKGCKADAKGCPSDLDHDG
                     VCDGLDRCADTPLGARVDVQGCPIDSDGDGVPDGIDQCADTPKGCKVDGKGCTLDTDN
                     DGVCDGIDRCPSTPAGATVDAKGCELDGDKDGVPDGIDHCPGTPVGCVVDATGCSVDT
                     DGDGVCDGLDKCPNTPKGRPVDLTGCEFVLPTPEKPLVLEGVHFETGKALLTEDSKQI
                     LARVAASLLDHPEVKLEVAGHTDATGSDAHNLKLSQARANAVRNYLISKGVKAEVLTA
                     KGYWKSQPIADNQTKEGRDRNRRTELRRLP"
     gene            complement(2504..>3974)
                     /locus_tag="AC16GM_08705"
     CDS             complement(2504..>3974)
                     /locus_tag="AC16GM_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923792.1"
                     /translation="FNPARGDSILIVYGTQGDSATTAYLKFLAARTFWIVANEKPELK
                     MAVVPDMSITPPRGLEVSLVVLGTKRSNRILKEYERPLQVRVLPDQVFIGLNQFLGKD
                     YTVAFVHPSPFNPFKYLLVLTGSSDSTLAMLPQIPDTFDFCLKQVHQYEKNRYYTAGF
                     FAKGTLPWGLEEGAVKVTGKYRHYRVLLSPKLRLRYPRDWDTLLTPPQKLWLLRTAQK
                     THDVFAITYGFNLPRPFDLCLVDDLPGTTATDYARLYWKIDPLHLDTTVAPATGIALA
                     RFILSCPDAPSDWPTFVQSTGVAPATVFRRAKVIDSATVERPKPKEQVWFEEIVAPDT
                     TYASILFAAANAYQPRALTSVINQVTHTLENPFVPMSTLLERLAARTGDRVIAQLARQ
                     AIPLPYDKPIRIRTGIPNLADHLQKDRVLTLASLPSSGAAAAAGMKKGDTLLSIDGIS
                     LDFQRALAYQRLAGKKKGDRILFGIQRGEKTLNIPITAR"
BASE COUNT          848 a         1055 c         1252 g          819 t
ORIGIN      
        1 ggcggggttc attgaccact tcttaggccc cggttgtact ctggaagatt tcgcccgcgc
       61 ccgctatccg gaagaggggg attttgtcaa tcagccatat gaggcggtgt tcaaaaccaa
      121 aggccaaaaa agcacggtga ctttcaaacg ggacggaaaa gtctgggttg gcagcaagca
      181 aatacctctg cgggtagaga aagagttaac gtttgaagcg aggcgttcag gtttcgacgt
      241 ttcctaccgg ctatccaatc tcagcgacca ggcggtggaa ctctggttcg ggatggaact
      301 gaatttaggt ttgttagccc ccaacgcgcc ggatcgatac gtgctcatca acagtcagag
      361 accagcggaa gtgaatttcg cggctaccgg ttcgtacgtt gccgtcagaa cggtcgcgct
      421 ggtggatgag tggcggaagg tgcagattga attaggcctc aaagagccgg tagtgctctg
      481 gcgatttccg atttacaccg tctcgctctc cgaagggggt atggaaaagg tgtttcaggc
      541 ggcttcgttt ctctttcacc gacggctcac actggctccc caggagcaac gggtctggct
      601 tctggcggtc accctgtcat cgctttgagc catctgcctt ttcgtcgggg gctgaacttt
      661 tttgcagaat tgtcgatatt caatgtggtt gacagccgag cgggataggt tattatcttg
      721 tcttctattg cgggaggcaa ggcacgcagg caaggaggca aaatgcgtag gcaactcttc
      781 ggactgatcg gttcatttct tcttctgaca cccgtcattc acgcccagga tatcgacggc
      841 aaattcggtt tgcgcattca tctgggagcg tggaagtcga tccagaccga ccacagcgat
      901 atctggacca tcgggccttt gggccggttc gggatcgact acgccgtcgc cgattttttt
      961 accatcggtg tagccggcgg ctaccggctg gtgtatgaag cgaatttagt cgggcgtacg
     1021 aaaggcgccg ggtttacgtt cgacaaactg agcggcggta atacctttcg gaaccagctg
     1081 tacgaactga cgttcggcct gcggccgctc ccgcacgcca agatagcgcc ctactttgcc
     1141 aacggcgtgg ggttcaactt ctggcaagtg aaacgtacgg ggggaactct ggattcgctc
     1201 accgaccgca acgggaactc attcaaggcg caggacaaag aattgacctt gagcaccgga
     1261 ttgggcgtgg aacttttccc caccgaaaat ctggcaattg acttgggcgg gcgctaccac
     1321 taccgaacga aggctttctc ggatttgaaa ggcgcaaagg acgtctggga tgcggcattc
     1381 gaagacctgc cgcgcgggct gttcgaaacc tacatcggcc tggtcgttta attcggcaag
     1441 ccgaaagatg ccgatagcga tggggtaccc gaccggaccg acaagttccc cggcacgccg
     1501 gcgggctgtc aagtggatgc cctgggctgt tccattgatt cggacggtga cggcgtttgt
     1561 gacggcgttg accagtgccc gagtaccccg aaagggtgta aagcagatgc aaagggatgt
     1621 ccgagtgacc ttgaccacga tggggtctgc gacgggctcg accgctgcgc cgatacgcct
     1681 ttgggagcgc gggtcgacgt gcagggctgt ccgattgatt cggacggcga cggcgtcccg
     1741 gacgggattg accagtgcgc cgacaccccc aaggggtgta aggtcgatgg caagggctgt
     1801 accctcgaca ccgacaatga tggcgtgtgc gacgggatcg accgctgccc gagcacgccg
     1861 gccggcgcca ccgtggatgc aaaaggatgc gagttggacg gcgacaaaga tggtgtacca
     1921 gacgggattg accattgccc ggggacaccg gtgggttgcg ttgttgatgc caccggctgt
     1981 tcggttgaca ccgacggcga cggcgtctgc gacggcctgg acaaatgtcc aaacacaccc
     2041 aaaggcagac cggtggacct gaccggctgt gagtttgtgc tccccacgcc cgaaaaaccg
     2101 ctggtgctgg agggcgtgca cttcgagacc ggcaaagcgt tgctcaccga ggattccaag
     2161 cagattctgg cccgtgtcgc cgccagtttg ttggaccacc cggaggtgaa actcgaagta
     2221 gccggacaca ccgacgccac cggctccgac gcgcacaatc tgaaactctc gcaggcgcgg
     2281 gcgaacgcgg tacggaatta cctcatcagc aaaggggtca aggcagaggt gctcaccgcc
     2341 aaaggatatt ggaagtccca gcccatagcg gataaccaga cgaaagaagg gcgggaccgg
     2401 aaccgccgga cggaattgcg gcggctgcct tagaccggcg atttaaagcg gaactgactt
     2461 atcaaaccca gaatcccgtc cccaagacgg gattctgggt tttttagcgg gcagttatcg
     2521 ggatgttcaa ggtcttttcc ccgcgctgga tgccaaagag aatccggtcg ccttttttct
     2581 ttcccgccag ccgctggtac gccagagcgc gctggaaatc tagggagatg ccatcaatgc
     2641 tcaggagggt atctcccttt ttcattccgg cggctgcggc ggcgccggag gagggcaaag
     2701 aagccagcgt gagcacccgg tctttttgta aatgatcggc caagttcgga ataccggtac
     2761 ggatgcggat cggtttatcg tagggcaggg gtatcgcctg ccgggccagt tgagcgatta
     2821 ctctgtcgcc ggtccgcgcc gccaaccgct ccagaagtgt actcatgggt acgaacggat
     2881 tttcaagggt gtgcgtcacc tgatttataa cactggtcaa ggcccgcggc tggtaagcgt
     2941 tggctgcggc aaaaagtata ctggcgtagg tcgtgtcggg agcgactatt tcttcaaacc
     3001 agacttgctc tttaggtttg ggccgctcga cggtggctga atcgatgact ttggcgcgcc
     3061 tgaagacggt ggccggagcc acacccgtag attgtacaaa ggtaggccag tcgctgggag
     3121 catccgggca ggaaagaata aaacgggcca gggcgatgcc ggtggccggt gccacagtcg
     3181 tgtccagatg gagggggtca atcttccagt agaggcgggc gtagtcggtc gccgtcgtgc
     3241 caggaaggtc gtccaccaag caaaggtcga acggtcgggg caggttgaat ccgtaggtaa
     3301 tcgcgaaaac gtcgtgggtt ttctgggcag tgcgtagcaa ccaaagtttc tggggaggcg
     3361 tcaggagcgt atcccaatcc cgagggtagc gcagccggag tttgggcgac aggagcaccc
     3421 ggtaatgacg gtacttgccg gtgaccttca ctgctccttc ctccaacccc cagggcaggg
     3481 tgcctttggc gaaaaaacct gcggtgtagt agcgattttt ttcatactga tgcacctgct
     3541 tgaggcagaa gtcaaaagta tcgggaattt gcggcaacat cgccagggtg ctatccgaag
     3601 agccggtgag caccaaaagg tacttgaacg ggttaaaggg gctggggtgg acgaacgcga
     3661 ccgtgtagtc tttgcccagg aactggttga gtcctatgaa cacctgatcg ggtagcaccc
     3721 gaacctgcag tggccgttcg tattctttca ggattctgtt ggagcgcttt gtgcccagca
     3781 ccaccagaga aacttccaga ccccgcggcg gcgtgatgct catatccggc acgacggcca
     3841 ttttcaattc cggtttttcg ttcgccacga tccagaaggt ccgggcggcc agaaacttca
     3901 agtaggcggt cgtcgccgag tcaccctggg tgccgtagac gatgagaatc gagtcaccgc
     3961 gcgccggatt gaaa
//
LOCUS       DBNGZD010000324         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1785909, whole genome shotgun sequence.
ACCESSION   DBNGZD010000324 DBNGZD010000000
VERSION     DBNGZD010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1785909"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..526)
                     /locus_tag="AC16GM_08710"
     CDS             complement(137..526)
                     /locus_tag="AC16GM_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923793.1"
                     /translation="MLADTAASAGPARAGTPTGIGYSLYLAHHYSKAVGGGPAVNQLR
                     WSLDLTLSARWSLRYTQSYDFTGRQVAAQQLNIHRDLHCWEADFSWIPAGPSEGYYFV
                     IRVKALPEIKLEKSRSGLGDILSNFVQ"
     gene            complement(575..>1945)
                     /locus_tag="AC16GM_08715"
     CDS             complement(575..>1945)
                     /locus_tag="AC16GM_08715"
                     /inference="COORDINATES: protein motif:HMM:NF040057.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HIR2923794.1"
                     /translation="KIVAKDKVVAKPVFFYVAGIPLFGVPYYLFPIKPGRHSGFLTFR
                     IGDFAQGRRGVRDLGYYWAFSDYWDLESAVDLTEGGGNLSLLWRNRINYAWRYHLSGS
                     VSGSYNRSSSGFGAAKTNRWDLTVNHFQELSPTARLTAAGSFVSDKSYYPIFANTLNQ
                     RLNRVLNPRASLTKSFGSGVSVFAAGEFVRNLDTDARTLTLPTLSITKPQRPLFSPKT
                     EPGQIPIRRWYHSLYYSYGGSAINFSSRTPTASGKLVKHFATATQEVNLSTPQAILRY
                     IAVTPNFNYRETWINVFPSNAADSAGVPTNSPARRGTYSLGIGALTNLYGTFPVKIGG
                     FQGLRHVVTPQISFAYTPKFTRNALLASYVGQPSTTARSEVMGFSLGNLLQIKTRHRD
                     QERKLEVANLNFSSGYNFTLTQRKLAPLVSSFRTTAVPAFGFEFNSVHDFYDSRGNLR
                     RPKLLN"
BASE COUNT          400 a          544 c          624 g          377 t
ORIGIN      
        1 tcatgctctc acccccttag atcggcaaag ggtttcacgc tccatactcc gcgacctgtg
       61 cggcgagaat agcgttcggg tgcgggtttg tcaagacatt tttgggggaa ttgcgcaatt
      121 ttttccgctc ccccggttac tggacgaagt tggagaggat gtcgcccagc ccggagcgcg
      181 atttctccaa tttgatttcg ggcagggcct tcacccggat cacaaagtag tacccttcac
      241 tcggccccgc ggggatccag gagaaatcgg cctcccagca gtgcaggtca cggtggatat
      301 tgagctgctg cgccgctacc tgccggccgg tgaagtcgta gctctgcgtg tagcggagac
      361 tccagcgggc cgagagggtg aggtccaacg accagcgcaa ctggttgact gcggggccgc
      421 cgcccaccgc cttggagtaa tggtgcgcca ggtagaggct ataaccgata ccggttggcg
      481 ttcccgctct ggccggtccg gctgatgcag cagtatccgc aagcaaagtt ccaccgccca
      541 gagtgccacc ccgggagaag gtggacgtgg tggactaatt caaaagctta ggccggcgga
      601 gattcccccg gctgtcgtag aaatcgtgca ccgagttgaa ctcaaaccca aaagccggca
      661 ccgcggtggt gcggaaactg gaaaccaaag gcgccagttt ccgctgcgtg agcgtgaagt
      721 tgtaaccgga ggaaaaattg agatttgcga cttccagctt ccgctcctga tctctgtgcc
      781 gggttttgat ctgaaggaga tttccgaggg aaaaacccat gacctcgctt ctggcggtcg
      841 tggagggctg gccgacgtaa ctggccaaga gggcgttccg ggtgaacttg ggtgtatagg
      901 cgaaactaat ctgaggcgtc accacgtggc ggagcccctg aaacccgccg atcttcaccg
      961 gaaaggtgcc gtaaaggttg gtcagtgctc cgatcccgag actgtaggtt ccccgccgag
     1021 ccgggctgtt cgtcggcacc cccgcagagt cggccgcgtt ggaggggaac acattgatcc
     1081 aggtttcccg gtagttgaag ttcggcgtca ccgcgatata gcgtaaaatc gcctgcggag
     1141 ttgacagatt cacctcctgg gtggccgtgg cgaagtgctt gaccagtttc cctgaggccg
     1201 tgggcgtccg actggagaaa ttaatggcgg aaccgccgta ggagtagtag agcgaatggt
     1261 accagcgccg aatcggaatt tgtcccggct cggttttcgg gctgaagagc ggccgctggg
     1321 gcttggtgat cgaaagggtg ggcaacgtca acgtccgcgc atcggtgtcc aggttgcgga
     1381 cgaactcgcc ggcggcaaag acgctcacgc cgctcccgaa cgacttcgtc agactggccc
     1441 gcgggttgag cacccggttc agcctttggt tgagcgtatt ggcgaagatc gggtaatagc
     1501 tcttgtcgct cacgaaactg ccggcggcgg tcaggcgggc cgtgggcgac agttcctgga
     1561 agtggttgac ggtgaggtcc cagcgattgg tttttgcggc gccgaagccg ctactggagc
     1621 ggttgtacga accggaaacc gaaccggaca ggtgatagcg ccaggcatag ttgatccggt
     1681 tccgccagag taaagaaagg ttcccgccgc cctcggtgag atcgaccgcg ctctccaaat
     1741 cccagtagtc ggaaaaagcc cagtagtagc ccaaatcccg cacgccccgc cggccctggg
     1801 cgaaatcacc gatgcgaaag gtcagaaacc cggagtgccg gccgggctta atgggaaaaa
     1861 gatagtaggg caccccgaag agcggaatgc ccgccacgta gaagaagacc ggtttggcca
     1921 ccactttatc tttggccacg atttt
//
LOCUS       DBNGZD010000325         4393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1372471, whole genome shotgun sequence.
ACCESSION   DBNGZD010000325 DBNGZD010000000
VERSION     DBNGZD010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1372471"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="AC16GM_08720"
     CDS             complement(<1..355)
                     /locus_tag="AC16GM_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923795.1"
                     /translation="MQKRVNWFLCIFSVTLFCALAPTLSGWAWRHPAYVANRPPTPFS
                     LLLPPNGDTIRTSRPTFLWQSSSDPDPGDQVSYVLQITQDSTFALVPITVPVLGGDTA
                     FTPPVPLFVNNFEIWF"
     gene            complement(587..2152)
                     /gene="ltaE"
                     /locus_tag="AC16GM_08725"
     CDS             complement(587..2152)
                     /gene="ltaE"
                     /locus_tag="AC16GM_08725"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: protein motif:HMM:NF007825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HIR2923796.1"
                     /translation="MKLIDLRSDTVTKPSPEMRQAMYDAEVGDDVFEDDPTVQKLEEK
                     VAALFGREASLFVPSGTMGNQVALRTWARPGDELLLEEGAHIFNYEVGGAAALTGLVL
                     HPLPGRNGVLSAEQIEAALRPENIHHPRTRLVSLENTHNRAGGTIYPLTEIQKIRELA
                     QTHGLKMHLDGARIWNAAVAVGVPLSLYGSLFDSVMTCLSKGLGAPIGSVVIGDRDYI
                     AEARRQRKMLGGGMRQVGILAAAGIYAIDNNFPRLAQDHEHAKFFARALAEIPGLKVD
                     LATVETNIVVIDISPSGLAVSEAVLKLKAEGVLVVPFGAQRIRAVAHLDATREDFETA
                     LSVFRRLFAVRAVPVVRPLLAQEPLTNGLQAVSGEPEPVAVEIKYRLPIDLARLRQAF
                     TDGTQETNYFLSLRTAEIKMISYLSREEERDKILATRNEYLLIAKKNPKDGYRDMEDF
                     IAGLTGEALKERLRSAIQGKGAFRRFKDVLMGYPEERRQWFRFSEERDFVRLRRWLGQ
                     EGIAVAEEQVAAQ"
     gene            complement(2348..2551)
                     /locus_tag="AC16GM_08730"
     CDS             complement(2348..2551)
                     /locus_tag="AC16GM_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2923797.1"
                     /translation="MHKGRVKWFNEVRGYGFITEEENGQDLFVHFADIQERGYRTLAE
                     GQEVAFEVTVGDKGPQATNVSKV"
     gene            complement(2683..4110)
                     /locus_tag="AC16GM_08735"
     CDS             complement(2683..4110)
                     /locus_tag="AC16GM_08735"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HIR2923798.1"
                     /translation="MAETTPLSKLPEEQINTPGKLFRWPSIDALVLLLLALANAQNTH
                     VFNDKVVAILSQHGIFKSLGQLPTAPLILNDLVYAGVVACAFAYFFLAIIPKSRPKMV
                     STILFGTIILLEVGLPSAKQIIARRIEGPHTHAHDGGVIMIEEATKMFLRGENPYTQD
                     FSTTPMGKWGVDTATGLTGWEKYGYEKNPPLTHFAFPPGNVLLTVPFSLVSRKLFGFY
                     DQRFLYLFLYLASLFLLAAGVREANRRSIMLVVFALNPLVVPFIVEGRTDIFVLFWVF
                     GFLSLLLKKRLFWASVFLGMACAVKNFAWFIVPFYLLYLLRPRIDSERGQSTKATFWQ
                     AVGPLFLTLALSCGPFLVWNFPAFWEDVVAYNSGYGSPNYPVKGPNFSSLLMALGLIK
                     SHSQYFPFFLLQILFAVPTFLFFLRRQWQRNTLSQLVIGSTFTLFVALFFNRYLSDNY
                     LGYMTILLALGFVLREENAGIQISS"
     gene            complement(4185..>4393)
                     /locus_tag="AC16GM_08740"
     CDS             complement(4185..>4393)
                     /locus_tag="AC16GM_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR2923799.1"
                     /translation="KNGPLRLEGEFTICDPQGSSYGLAGRTVISLCRCGNSANKPFCD
                     GSHNRVGFQHEPAAFELPPMKPKV"
BASE COUNT         1013 a         1237 c         1228 g          915 t
ORIGIN      
        1 agaaccagat ctcgaagtta ttgacaaaaa gcggtacggg cggggtgaag gcggtgtcgc
       61 cgcccagaac cggcacggtg atcggaacga gagcaaacgt gctgtcctgg gtaatctgga
      121 ggacgtagct gacttgatca ccaggatcgg ggtccgacga cgactgccag aggaacgtcg
      181 gccgactggt gcggatagtg tcgccattcg gaggtaaaag aagcgaaaag ggagtcggtg
      241 gccgattggc aacataggcg ggatgccgcc aggcccagcc ggagagcgtc ggtgccagag
      301 cgcaaaagag cgtgaccgag aaaatgcaca aaaaccaatt gacccttttc tgcataccaa
      361 tcacatctcc gccggtgccc gtgcaccggc tttctcccag cacttccttg cttggaaacc
      421 tgagctccgc tttcccttag aggtcagaga gaagggggaa agcgatctac agatacgcta
      481 tctcctaagg aattgcaacc agtctgccgg gcagagaaaa atctaaccca ttgtggcata
      541 agaggttacc cattggtgcc gggcgtgcgg gcgaaatact gttcagttat tgagcagcca
      601 cttgctcttc ggcaaccgca attccttcct gacccagcca gcggcgcagc cgcacgaaat
      661 cccgctcttc ggaaaagcgg aaccactggc gccgctcctc cggataaccc atcaagacgt
      721 ctttgaaccg ccggaacgcc cctttgcctt ggatggcgga gcgcagcctt tctttcagcg
      781 cctcaccggt gaggccggcg atgaaatctt ccatgtcccg gtagccgtcc ttggggtttt
      841 tctttgcgat cagcagatat tcattccggg tggccaggat tttgtcgcgc tcttcttccc
      901 gggaaaggta gctgatcatt ttaatttcgg cggttctcag cgacaaaaag tagttggttt
      961 cctgcgtccc gtcggtgaag gcctgccgga gtcgggcgag atcaatcggc agtcgatatt
     1021 tgatctccac ggccaccggc tcgggctcac cggacacggc ctgtaacccg ttcgtgagag
     1081 gttcctgcgc taaaagtggt cgtacgaccg gtaccgcccg caccgcgaac agacggcgga
     1141 aaacactgag ggcggtctca aaatcttctc tcgtggcgtc caagtgcgcc acagcccgga
     1201 tgcgctgggc gccaaagggg acgaccagta ccccttcagc cttaagtttc aacaccgcct
     1261 cggacaccgc caagccgctg ggggaaatgt cgatcaccac gatattggtc tctacggtgg
     1321 cgagatcgac tttcaggccg ggaatctcgg ccagtgcccg agcgaaaaat ttcgcgtgct
     1381 catgatcctg ggccaggcgg ggaaaattgt tgtcgatagc gtagatgccc gcggccgcca
     1441 gaatccccac ctgccgcatc ccgcccccca gcatcttccg ctggcgccgc gcttcggcga
     1501 tgtagtcgcg gtcgccgata accacggaac cgatgggcgc tcccaggcct ttggaaagac
     1561 aggtcatgac cgaatcaaag agcgacccgt acagggaaag cggtaccccg acagccaccg
     1621 cggcattcca gatccgggcg ccgtccaagt gcattttcag tccgtgcgtc tgcgccaact
     1681 cccggatttt ctggatttcg gtcaaagggt aaatcgtccc tccagcccgg ttgtgggtgt
     1741 tttccagcga gacgagccgc gtgcgcgggt ggtggatgtt ttccggccgc agtgccgctt
     1801 cgatctgctc ggcggaaagc acaccgttcc gacccgggag cgggtgtaaa accaaaccgg
     1861 tgagggcggc cgcccccccc acctcgtagt tgaagatgtg agcgccttct tctaagagga
     1921 gttcatcccc gggccgggcc caggtgcgca gggccacctg attgcccatc gtgccggagg
     1981 ggacaaaaag tgaggcttcg cggccgaaca gggccgccac tttctcttcc agtttttgga
     2041 cggtgggatc atcttcgaaa acgtcatcgc caacttcggc gtcgtacatc gcctggcgca
     2101 tttccggcga cggcttggtc accgtgtcgc tgcgcaaatc tattagtttc attgtccctg
     2161 cctctttcag tcattgccat tctcatttgg attttctcat cttaaagctg cttcgcttta
     2221 tcttactgag gaatgaccct cgtcgtcttc cgctctctcc tttcaccctt ccggatattc
     2281 acgggtaacg aaaaatcccc cgccctggcg ggggatcttc cgggctcaga gccacctcgt
     2341 caaggtccta cactttgctc acgttggtcg cttgcgggcc tttgtcgccc accgtgacct
     2401 cgaacgctac ctcctgacct tcggccaggg tccggtaccc ccgctcctgg atgtccgcga
     2461 agtggacgaa cagatcctgg ccgttctcct cctcggtgat gaatccatac cctctcactt
     2521 cgttgaacca cttcaccctg cctttgtgca tcgcacgctc ctccctcaga gcagtttcta
     2581 cgctgacaac atctggcacg gcttgcatct gtatacttac aaactacagc ccggaatccg
     2641 gccttgtcaa gaaaaaaaga tgttctatta accggttatg gttcaactac ttatttgaat
     2701 tccggcgttt tcttcacgca ggacgaaccc gagcgccaaa aggatggtca tataccccaa
     2761 gtagttatcc gaaaggtagc ggttaaaaaa taaagcgacg aacaaagtaa aagtcgaacc
     2821 gataaccaat tggctaagtg tgttccgctg ccactgccgc ctgaggaaaa aaagaaacgt
     2881 cggcaccgca aagagaatct gcaaaaggaa aaaggggaaa tactggctgt gggatttgat
     2941 gaggcccagg gccatgagca atgatgaaaa attcggccct ttgaccggat agtttggact
     3001 gccgtagccg ctgttatagg caaccacatc ctcccagaag gccgggaaat tccagacgag
     3061 aaaaggaccg caggaaagtg ccagcgtcaa aaaaagtggc ccgaccgctt gccagaaggt
     3121 tgcttttgtg ctttgacctc tttcagagtc aattcgtggg cggagcagat aaagaaggta
     3181 gaagggcacg atgaaccagg caaagttttt gacggcacaa gccatgccaa gaaagaccga
     3241 agcccagaac aggcgcttct tcaataggag ggaaagaaat ccaaacaccc aaaagagaac
     3301 gaaaatatcg gtgcgaccct cgacgatgaa gggaaccacc aggggattaa gcgcaaaaac
     3361 gacgagcatg atcgagcgac ggttcgcctc tcgaacccct gcggccagaa ggaaaagact
     3421 ggcgaggtaa aggaagagat acaagaaccg ttgatcgtaa aacccgaaga gttttctgct
     3481 gacgagggag aagggcaccg tcaaaagcac gtttcccggg gggaaggcga aatgagtcag
     3541 ggggggattt ttctcatagc cgtacttttc ccagccggtg aggcccgtgg cggtatcgac
     3601 gccccatttt cccatcggtg tggtcgagaa atcttgggta tatggatttt ctccccgcaa
     3661 gaacatcttc gtcgcctctt cgatcataat gaccccgccg tcatgggcat gcgtgtgcgg
     3721 cccttcaatc cgccgggcga tgatttgctt ggccgagggc aaccccactt cgagcagtat
     3781 gattgtacca aaaagaatgg tggagaccat cttcggacgg cttttgggaa tgatggctaa
     3841 aaagaaatac gcgaaggcgc aagcgacgac cccggcgtag accaggtcgt tgagaattaa
     3901 aggcgccgtc ggtaactgac ccagactttt gaaaatcccg tgctgggaca gaatcgcaac
     3961 gactttatcg ttgaaaacgt gcgtattctg cgcgttggcc agagcgagaa gcagaaggac
     4021 taaagcgtca attgaaggcc aacggaacag tttgcccgga gtgttgattt gctcttcggg
     4081 caatttactc aggggggtag tttcagccac attgctaact ggggtgaagc cctgcaaact
     4141 caccggcgtt tgcatggccg accgggacgt ttttttactc ttccttatac cttcggtttc
     4201 atcggcggca gctcgaaagc cgccggctcg tgctggaagc cgacgcggtt atgactcccg
     4261 tcgcagaagg gtttgttggc cgaattgccg caccggcaga gcgaaatgac cgtgcgccca
     4321 gccaaaccat aggagctgcc ctgcgggtcg caaatggtaa attccccctc caatcgcagg
     4381 gggccatttt tgt
//
LOCUS       DBNGZD010000326         2219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1927557, whole genome shotgun sequence.
ACCESSION   DBNGZD010000326 DBNGZD010000000
VERSION     DBNGZD010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1927557"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /gene="lon"
                     /locus_tag="AC16GM_08745"
     CDS             <1..2058
                     /gene="lon"
                     /locus_tag="AC16GM_08745"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR2923800.1"
                     /translation="RRYPGEFYHIFRLNAHDPSRFADVIASTFHLVLGTKLKILETTD
                     VKARLEKLLGYIDDEIMRLELSAKIEAGVKVNLEKIQREYYLRQQLREIKRELGESDP
                     QEEDVERLTRLVGQSSLPQAVQRQAILEIERLRSLSTASAEYGITRNYVEWLLALPWR
                     ADCPTEVDLESLEKTVAETFYGLEKVKTRLLENFSVRKLKPDYRGAVLCFAGAPGTGK
                     TSLAETIARALGRKFVRLNVGGLDDDSDILGERRSYVAAHPGRILEALRESGVCNPVI
                     LIDEIDKIGNATTHGDITGALLSVLDPELNRRFLDRYLGFPYDLSPVLFLCATTSAEN
                     IPVPIAQKLELIELPGYLEDEKIAIAQKYLLPRLASEVGLEPGSLEVSDNAIRMLIRQ
                     YAVEAGLWALQRELETLTRKYARQRVASGPCQWKVTEKNLSKILGAPIYIPDMAEAKP
                     EVGVATGLAWTETGGDIMLIEALKMKGSGTVISTGSLGDVMKESIQASHSYVRAKADL
                     LGIRYEDFTSHDIHIHFPSGAVPKDGPSAGIAISLAIASVMANKAIRNDIAMTGEVSL
                     RGKVLPVGGIREKVSAALRVGIRTVVLPKQNEKDLEDLPPRLRKEMKFIPIEYTEEIF
                     PLAFVDFQPGLASLEQLLRQEVEKVRKKKTKENARERKAAKSARSRKPQPPEEPTGSI
                     SFV"
BASE COUNT          475 a          664 c          669 g          411 t
ORIGIN      
        1 cgccgctacc ccggagaatt ctaccacatt ttccggctca acgcccacga tccctcgcgc
       61 tttgccgacg tcattgcctc cacgtttcac ttagtcctgg ggaccaagct caaaattctt
      121 gaaaccaccg acgttaaagc ccgcctggaa aaactcttgg ggtacattga tgacgaaatt
      181 atgcgcctgg aactttcggc gaagattgag gccggggtca aggtcaactt ggagaaaatt
      241 cagcgggaat actacctgcg ccagcaactg cgggagatca agcgcgagct gggggagagc
      301 gacccgcagg aagaggacgt ggagcggctg acgcgtctgg tcggtcaatc gtccctgccg
      361 caggcagtgc agcgacaggc gattctggaa attgagcgcc tgcgctcgct ttccactgcc
      421 tcggcggagt acggcattac ccgcaactac gtcgagtggc tcctggcgct gccctggcgg
      481 gccgactgtc cgacggaggt cgatctggaa agtctggaaa agaccgtagc ggaaaccttc
      541 tatgggttgg aaaaggtcaa gacccgtctg ctggaaaact tctcggtccg caagttgaaa
      601 ccggactacc gcggcgcggt cctctgcttt gccggcgcac ccggcaccgg gaaaacgtct
      661 ctggccgaga ccattgcccg ggcgctgggg cggaaattcg tccggttgaa cgtcggcggt
      721 ttagatgacg actctgacat tctgggtgag cgccgctcgt acgtagccgc ccacccgggc
      781 cgcatcctgg aagcgctgcg ggagtcgggt gtctgcaacc cggtgattct catcgacgaa
      841 atcgacaaga tcggaaacgc caccacacac ggcgacatca ccggggcact gctgtcggtc
      901 ctggatccgg aactcaaccg gcgctttctg gaccgttacc tcggattccc ctatgatctt
      961 tcgcccgtgc ttttcctctg cgcgacgact tccgcggaga atattccggt gccgatcgcg
     1021 cagaagctgg agctcatcga actccccggc tacctggaag acgaaaaaat cgccatcgcg
     1081 cagaaatatc tgttgccgcg cctggcgagc gaggtggggc tggaaccggg ctctctggag
     1141 gtgagcgaca acgccatccg gatgttgatc cgtcagtacg cggtggaagc cgggctctgg
     1201 gcgctccagc gggaattgga aaccttaacc cgcaagtatg cacgccagcg ggtggcctcc
     1261 ggtccctgcc agtggaaggt gaccgagaag aatctctcca aaattctggg ggcgccgatt
     1321 tacattcccg atatggcgga ggccaagccg gaagtggggg tggccaccgg tctggcctgg
     1381 accgagaccg gcggcgacat catgctgatc gaagcgctga agatgaaagg ctccggcacg
     1441 gtcatctcca ccggctctct gggtgacgtg atgaaagaat cgatccaggc ctcccactcc
     1501 tacgtgcgcg ccaaagccga cctattgggc atccgctacg aagatttcac ctcccacgac
     1561 atccacatcc acttcccttc gggagcggtt cccaaagacg gcccctcggc cgggatcgcc
     1621 atatcgctcg ccatcgcgtc ggtgatggcc aacaaggcga tccgcaacga catcgccatg
     1681 accggcgagg tgtctctgcg gggcaaagtg ctgccggtgg gggggatccg ggagaaagtc
     1741 tccgccgcgc tccgggtggg gatccggacg gtggtcctgc cgaagcagaa cgaaaaagat
     1801 ttggaagatc tgccgccgcg cctgcgcaag gagatgaaat tcattcccat cgagtacacc
     1861 gaagaaatct tccctttggc gttcgtggac ttccagccgg gtttggccag tctggagcaa
     1921 ctgctgcgcc aggaagtgga aaaagtccgg aagaaaaaaa ccaaggaaaa cgcccgggag
     1981 cgcaaagccg ccaaatcggc ccgcagccgg aaaccgcagc cgccggaaga gccgaccggc
     2041 tcaatcagct ttgtttgaat ttttctcccg gagtcccctc ctttccttcc caaagtcagc
     2101 cctccttttc gttactggag ttaccaccgt ctgccgaaga gcggcacggg aaagagaatt
     2161 gacttcccgg tcactgcgtt gtagcgtatg caggcaaagc gattgatagg gaagagagt
//
LOCUS       DBNGZD010000327         1513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1511434, whole genome shotgun sequence.
ACCESSION   DBNGZD010000327 DBNGZD010000000
VERSION     DBNGZD010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1511434"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1244)
                     /gene="ribD"
                     /locus_tag="AC16GM_08750"
     CDS             complement(141..1244)
                     /gene="ribD"
                     /locus_tag="AC16GM_08750"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR2923801.1"
                     /translation="MRQTFALAQKGWGYTSPNPLVGAVVVKNGRIMGQGYHRKAGEAH
                     AEVIALAAAGKKARGATLFVNLEPCCHYGRTAPCTEAILSAGVKKVVLSVIDPNPLVN
                     GKGIRTLERHKVAVVTGVLEDAARQLNEVHFKFITTGLPFVIIKVAQSWDGRLATLTG
                     DSQWISGEKARRFAHLLRSRVDAVLVGRDTVAADNPRLTVRLVKGNNPYRIVLDSVGR
                     VLPQSRLVKENQDGKTIWTRAKNGQATIDAMKGVKIWELPAGENGWIDLPAFLRQAGR
                     EEITSLLVEGGSRVVTSFLRQQLADKIYFCFGPLILGEGKDTVGDLNVRRLSEGIRLR
                     EVGMRKLAQDILVWGYPAWPDGSSQPKNRPSEN"
     regulatory      complement(1329..1454)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT          305 a          475 c          385 g          348 t
ORIGIN      
        1 acgccatcaa tactgaccga atcgccgacc gccagcgact gccgcactga ggtggagcaa
       61 atggtgagac gcatacctcc cccctgccgg gcgacctgct caactctgcc gatttctctc
      121 acaatgccgg tgaacattgc ttaattctcg ctcggccgat ttttcggttg agatgacccg
      181 tccggccaag ccggataacc ccagacgagg atgtcctgag ctaatttccg cattcccact
      241 tcacgcagcc gaattccttc gctcaacctc cggacattca ggtcgccgac cgtgtctttc
      301 ccctcgccca gaatgagcgg cccaaagcag aagtagattt tatccgccag ctgttgccgg
      361 agaaatgagg tgaccacgcg gctcccgcct tctaccagaa gactggtgat ctcttcccgt
      421 ccggcttgcc gcaaaaaagc gggtagatcg atccacccgt tttctccggc gggcaattcc
      481 cagatcttga cacctttcat ggcatcgatt gttgcctggc cgtttttggc tcttgtccag
      541 atcgtcttgc cgtcctgatt ttccttcacg agccggcttt gcgggagcac ccggccgacc
      601 gaatcgagca caattcggta cggattgttc cccttcacca gccgcaccgt caggcgggga
      661 ttgtcggcgg ccaccgtgtc acggcctacc agtaccgcat cgactcggct gcgcaaaaga
      721 tgggcaaaac ggcgcgcttt ttcgccagaa atccactgcg agtcgcccgt caaggtcgcc
      781 agccgaccgt cccacgactg ggcgactttg attatgacga acgggaggcc ggtggtaatg
      841 aatttgaagt gaacttcgtt gagctgcctg gccgcatctt ccaggacgcc ggttactact
      901 gcaactttgt ggcgctcgag tgtacggatt ccctttccgt tcaccaaggg gttgggatcg
      961 atcacactca aaacgacttt tttgacccct gcggagagga ttgcctcggt gcagggtgcg
     1021 gttcgcccgt agtggcagca gggttcgaga ttgacgaaaa gagtggctcc tcgggctttt
     1081 ttccccgccg ctgccagagc aatgacctca gcatgcgcct ctcccgcttt gcgatgatat
     1141 ccctgcccca tgattcggcc atttttcacg accacggctc ccaccaacgg attggggctg
     1201 gtgtagcccc accctttctg agccagggca aatgtttggc gcatgaagcc gtttacgtcc
     1261 ataaatgata taccccgatt cctctaacaa ccggacttcg gggcaaagaa agaagccgcc
     1321 aatcagatat cgaacttctt ccatccggac ttttaccgtc ggcacccgga tttcacgggt
     1381 tcagtccctc gttttagggg agtcgtgggc tttcaccacc gatcgaggaa ttccacctcg
     1441 ccccgaagtt ctttattagt atacgcaatc tgctggcttt gtcaatagcc cataaagcaa
     1501 aaagcccccc gcc
//
LOCUS       DBNGZD010000328         3506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1302897, whole genome shotgun sequence.
ACCESSION   DBNGZD010000328 DBNGZD010000000
VERSION     DBNGZD010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1302897"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="AC16GM_08755"
     CDS             complement(<1..243)
                     /locus_tag="AC16GM_08755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR2923802.1"
                     /translation="MERLSEFVLLTPSILFALTFHEFAHAWMANRLGDPTARDLGRLT
                     LNPLAHLDFFGTLMIFLSDFRFGWAKPVPFNPYNLKE"
     gene            complement(306..1598)
                     /locus_tag="AC16GM_08760"
     CDS             complement(306..1598)
                     /locus_tag="AC16GM_08760"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR2923803.1"
                     /translation="MTTLIETSPPELKSEYPHSSTLDNAMRQVDMAAQKLGMDPGFLE
                     IIKLPRRAVILNLPIQMDDKTFRVFTGYRVQHSIVRGPGKGGIRYHPDVTLDEVQALA
                     AWMTWKCAIANIPFGGAKGGIAVDPEKLSKGELERLTRRYIAELIEVIGPDKDVPAPD
                     VNTNSQTMAWVMDTYSMHARHTVTSVVTGKPVELGGSLGRREATGRGVMLTIREAAKH
                     LGMLLPGATVAVQGFGNVGSIAALELHKLGCKVIAASDVHGGIFNPKGLDIPQLIDHV
                     TRRKSVVDFPGAAAVSNEDLLTTRCDILVPSALENQITGENAEGIKAKIIGEGANGPT
                     TPEADTILDRKGVFVIPDVLCNAGGVTVSYFEWAQDRSGYFWSEEEVNTRLERILVKA
                     FADVLAMSLANKVNMRVAAFMVALRRVADVMHLRGMYA"
     gene            complement(1582..2205)
                     /locus_tag="AC16GM_08765"
     CDS             complement(1582..2205)
                     /locus_tag="AC16GM_08765"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR2923804.1"
                     /translation="MPTLEVARNLLGTYLVVNHRRKQLVGRIVETEAYLGLEDPASHA
                     YRGQTERNRVMWDEGGLVYVYFTYGMHHCLNVVTEAPGKAAAVLIRAVEPVRGVEEME
                     KRRRMRLGKLTALSKVEGRLGPDLTNGPAKLCSAFGIDKSFYGADLCGDHIWIEKRER
                     PWEPIGRSGRVGVNAASDQPWRFYLFGNRFVSKARPAREENNHDHAH"
     gene            complement(2716..>3506)
                     /gene="guaB"
                     /locus_tag="AC16GM_08770"
     CDS             complement(2716..>3506)
                     /gene="guaB"
                     /locus_tag="AC16GM_08770"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR2923805.1"
                     /translation="KDLLDRAAALVDAGVDLLVLDASHGHSKGVIEAVLKLKKRFPSV
                     PLVAGNVATGAATRELIAVGADSVKIGLGPGSICTTRVVTGTGVPQITAIMDCVEVAD
                     QHHIPLVADGGIKYSGDITKAIAAGASAVMIGSLFAGTEESPGETILFEGRTFKLYRG
                     MGSLEAMKKGSRDRYAQAEEEDPEKWVPEGIEGRVPYKGSLSETVFQLVGGLKAGMGV
                     CGAKAIEELRRKAEFVQITLAGLRESHPHDVTITKEAPNYRVNP"
BASE COUNT          710 a         1041 c         1030 g          725 t
ORIGIN      
        1 ctctttcaga ttgtatggat taaatggcac cggcttggcc cagccgaagc gaaagtcgga
       61 aagaaaaatc atgagcgtcc cgaagaagtc gaggtgcgcc agaggattta aggtcaggcg
      121 gccaagatcg cgggcggtgg ggtcgcctaa ccggttcgcc atccaggcgt gggcgaactc
      181 gtggaaggtg agcgcgaaga gaatcgaggg ggttaaaaga acgaattcag agaggcgttc
      241 cattcatccc tcaaaaagcg aaagtcgggg acatcccccg acttgcatct aaagacaaat
      301 cgaacttaag cgtacatacc ccgcaggtgc attacgtcgg cgacgcgcct gagggccacc
      361 ataaaggccg ccacgcgcat attgacctta ttcgccaggg acattgccag aacgtcggca
      421 aaggccttga ctaaaatccg ctctaaccgg gtgttcacct cttcctcgct ccagaaatag
      481 cccgagcggt cctgcgccca ctcgaagtag gaaacggtca cgccgccggc gttgcagagg
      541 acgtcgggga tgacgaagac gcccttgcgg tcgagaatcg tatcggcttc gggcgtggtg
      601 gggccattgg cgccttcccc gatgatcttg gccttgatcc cttccgcgtt ttctccggtg
      661 atttgatttt ccagcgccga gggcaccaga atgtcgcagc gggtggtcaa aaggtcttca
      721 ttggagactg ccgcggcgcc ggggaagtcc accaccgatt tgcggcgggt aacgtggtcg
      781 atcaattgcg gaatgtccag ccccttcgga ttgaagatgc caccgtgcac gtcggaggcc
      841 gcgatcactt tacatcccaa cttgtggagt tccaaagccg caatcgagcc gacgttgccg
      901 aagccctgca ccgccacggt ggccccgggc aaaagcatgc cgaggtgttt ggccgcttcc
      961 cggatggtga gcattacccc gcggccggtg gcttcccgcc ggcccaaaga accgcccagt
     1021 tccaccggct tgccggtgac gaccgaggtg actgtgtggc gggcgtgcat gctgtaggtg
     1081 tccatcaccc aggccatggt ctgggaattg gtgttcacgt ccggcgccgg cacgtccttg
     1141 tccgggccga tgacctcgat caattcggcg atgtagcgcc gggtgagccg ctccaattct
     1201 cctttggaaa gtttttcggg atcgacggca atgccgccct tggcgccgcc gaagggaatg
     1261 ttggcgatgg cgcacttcca ggtcatccag gcggctaagg cctgcacttc atccagtgtg
     1321 acgtcagggt ggtagcgaat gccgcctttg cccggcccgc gcacaatgga gtgctgcacc
     1381 cggtagccgg tgaaaacgcg gaacgtcttg tcatccatct gaatcgggag gttgagaatt
     1441 accgcacggc ggggaagttt gatgatttcc agaaaaccgg ggtccatgcc taacttttgt
     1501 gcagccatat ccacctgccg catggcatta tctaacgtcg aggagtgcgg atattcgctt
     1561 ttgagttccg gtgggctggt ttcaatgagc gtggtcatgg ttattctcct cccgcgcagg
     1621 gcgggctttt gagacaaatc tattgccgaa aagatagaag cgccacggtt gatcagaggc
     1681 ggcgttgaca ccgacccggc ccgaacgtcc aatcggctcc cacggccgtt cccgcttttc
     1741 aatccagata tggtcgccgc acaaatcggc tccgtaaaaa cttttgtcga tgccgaacgc
     1801 agagcagagt ttggccgggc cattggtgag atcaggcccc aaccgccctt cgactttgct
     1861 caaggcggtg agcttgccca accgcattct ccgccgcttt tccatctctt ccaccccccg
     1921 tacgggttcc acggcgcgaa tcaaaaccgc cgcagctttc cccggcgctt ccgtcactac
     1981 gtttaaacag tggtgcatgc cgtaggtaaa gtagacgtag accagaccac cttcgtccca
     2041 catcacccgg ttgcgctcag tctggccccg ataagcatga ctggccgggt cttcgaggcc
     2101 gaggtaggcc tcggtttcga cgatgcgccc gactaactgc tttctgcggt gattgacgac
     2161 gaggtaagtc cccagaagat tccgggcaac ctcaagcgtg ggcaaaaggt aaaaatcccg
     2221 cgtgagcttt tccttgaccg gtctttcgat ctctgatgtt ccaacgcttt tcggtagcat
     2281 ggatgacact gaataatacg aattagaaat cgtgagaaca agagggattc tccgagggag
     2341 aaatggtacc tgtgaatcgc gggggcgacc gagggcggtc gccctacgcg gtatactatg
     2401 tcagtggtag tcgggctcgt cccggagccc gacctgtcac cgaccggctc ccttcgggtc
     2461 tgagaacccg tcagggtcga agacagggac gagccggtct acccgtataa gaaagaagta
     2521 gggaggtgaa aactgagtga cctcaccccg gccctctcct acaggagagg gcgatcggag
     2581 agaaaaatgt tctctcccct ccaaccttct cttagcaggt agcatccttc caattcaaga
     2641 agagtatttt tctccctccc ctgtagggga gggtagggtg gggtcagaga aacggtcgac
     2701 tattccaacg gaactctacg gattgacccg gtagttcggc gcttctttgg tgatggtcac
     2761 gtcgtgcggg tggctttccc gcaagccagc taaggtgatc tgcacgaact cggctttgcg
     2821 ccgcagctct tcaatcgcct tggctccgca aacgcccatt ccggccttga gcccaccgac
     2881 taactggaaa accgtctctg agagcgaccc tttgtacggc acccggccct caattccctc
     2941 cggcacccac ttctccgggt cttcctcttc ggcctgggcg tagcggtccc ggctcccctt
     3001 tttcatcgct tcgagcgagc ccattccccg gtagagcttg aaggtgcggc cttcgaagag
     3061 aatcgtttcg cccgggctct cttcggtccc ggcaaaaagc gagccgatca taacggcgga
     3121 cgccccggcg gcaatcgctt tggtgatgtc tccggagtat ttgatgccgc cgtccgcgac
     3181 cagcggaatg tggtgctgat cggccacctc gacgcagtcc ataatggcgg taatctgcgg
     3241 cacgccggtg ccggtgacga cccgcgtggt gcagatcgaa ccgggcccca ggccgatttt
     3301 gacgctgtcg gcacctaccg cgatcaactc ccgggtggcg gcgccggtgg ccacattacc
     3361 cgccaccagc ggcaccgagg gaaagcgctt tttgagtttg agcaccgctt cgatgacgcc
     3421 ttttgaatgg ccgtgggagg catctaaaac caaaagatcg actccggcat cgactaacgc
     3481 cgccgcccgg tccaagaggt ctttgg
//
LOCUS       DBNGZD010000329         8394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_52931, whole genome shotgun sequence.
ACCESSION   DBNGZD010000329 DBNGZD010000000
VERSION     DBNGZD010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_52931"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="purM"
                     /locus_tag="AC16GM_08775"
     CDS             <1..768
                     /gene="purM"
                     /locus_tag="AC16GM_08775"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR2923806.1"
                     /translation="NDILVHGARPLFFLDYLATGKLDSTIVQQVITGLATACRTVGMA
                     LLGGETAQMPDFYQPGEYDLAGFIVGVVERKKIIDGKKIEPGDLLLGIPSNGLHTNGY
                     TLARKALFEIAGLNVNTILPELGEKLGDVLLRSHRCYWPVVKDLLPSGHLKGLAHITG
                     GGIAGNLVRILPEGCRAVIDRKTWPVPPIFEVIARRGNIGNEEMWKVFNMGIGMILVF
                     SPSKKKEVLAHFAKVKEKVFEIGRIETGKRSVELLPA"
     gene            779..1180
                     /locus_tag="AC16GM_08780"
     CDS             779..1180
                     /locus_tag="AC16GM_08780"
                     /inference="COORDINATES: protein motif:HMM:NF022317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR2923807.1"
                     /translation="MVYKILADSTIALHFGWIVFILGGFFLTVRAVFFRRHRYFFNRW
                     LLRSIHLGGILFVATLGLFGQDCPLTNVENALRYKADPTSIYTGSFIAHYLEQLVYPG
                     VNVIVLRAAAAGIALFTLVTYFALPPWRKHK"
     gene            complement(1199..1960)
                     /locus_tag="AC16GM_08785"
     CDS             complement(1199..1960)
                     /locus_tag="AC16GM_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923808.1"
                     /translation="MDIKKLIQLLGRSLPSQLVCDLVENFLILRQDVATGVLGRSAPG
                     KFVETFVQVLQFLEVGKFDAKPNVDEYLRTLESRSTSLDDGLRICAARIGRAMYALRS
                     KRNILHKGHVDPNTYDLKLLHSAAQWVLAELIRSLSGGSMEEAGKLVEQIQVPVGGLV
                     EDFGGRRIVLKDLLIAEEILVLMHSHYPETVTSVQIISSLYRRSPRSVRNTLRSLWKE
                     KILEGNNKIGYLLTRRGFDKAIAIVSQCIENGTGV"
     gene            complement(1964..2479)
                     /locus_tag="AC16GM_08790"
     CDS             complement(1964..2479)
                     /locus_tag="AC16GM_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923809.1"
                     /translation="MNDDSEIKAMASIKDALSDLDEKAIARVLRWAADRYNVFVGKEG
                     SKKGNGDIKGNGGNGNGSAFQDFASLYNAANPQTDAEKALVVGYWFQEVKSQEALDAQ
                     QMNSELKHLGYPIGNITKALYDLISREPRLAIQVHKSGATKQARKKYKLTTEGIRKAK
                     GMLENKPGSTE"
     gene            complement(2567..3523)
                     /gene="cysK"
                     /locus_tag="AC16GM_08795"
     CDS             complement(2567..3523)
                     /gene="cysK"
                     /locus_tag="AC16GM_08795"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR2923810.1"
                     /translation="MIFRDVTATVGRTPLVELGRLAKGLPGRMVAKLEMRNPCGSVKD
                     RLGVALIEDAERRGVLRSGMTLVEATGGNTGIGLAFAAAIRGYRLILTMPETMSKERV
                     ALLRHLGAEVVLTPGILMGDAIARAERFVKEIPGSIMLDQFKNPANPEVHRRTTAEEI
                     WEETQGAVDVFVSAVGTGGTITGVGEVLKARKPGVRIVAVEPAGAAVLSGGPAGNHQM
                     PGIGVGFIPEVLNRSILDEVIAVTDEDAFACTRRLAREEGILAGISSGAALYAALSVA
                     ARKEAKGKMIMVLLPDTGERYITTTLFASDAGNTKQSVNSCA"
     gene            complement(3588..4163)
                     /locus_tag="AC16GM_08800"
     CDS             complement(3588..4163)
                     /locus_tag="AC16GM_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR2923811.1"
                     /translation="MRTGRLEAFSDGVFAIIITIMVLGLTVPHKADLAALSPVGPVFL
                     SYLFSFVFLGIYWNNHHHLLQAIKHINGRVLWANLHLLFWLSLTPFVTGWMGQNRFAP
                     WPVALYGTVLLLAAVAYFILTRTLISLHGRDSVLATALGRDFKGKVSVAIYLLAIPLA
                     FVNSFLACALYVLVAVIWFVPDRRIEKTLAR"
     gene            complement(4410..5795)
                     /locus_tag="AC16GM_08805"
     CDS             complement(4410..5795)
                     /locus_tag="AC16GM_08805"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR2923812.1"
                     /translation="MRVPAWILGTDALVNAMDQGVYNQVTNVACLPGIQKVSICMPDG
                     HWGYGFPIGGVAAFDLKEGVISPGGIGFDINCGMRLVRTNLNYDEVKPKLEQLMHTLF
                     RLVPSGVGSKSSLRLSRQEFREVMVHGAQKIVKMGYGWPEDAERIEESGAIEGADPDQ
                     VSDKAFERGHSQMGTLGSGNHYLEVQVVREENLLDRETAGRMGITFPNQIVIMVHCGS
                     RGFGHQVATDYLRVFETAMRKYGIEVIDRELGCAPFSSPEGKNYFGAMVCAANAAFAN
                     RQVITHQIREGFSQIFGRDPHEMGMEIVYDVCHNIAKIEEYEINGKKKPLLVHRKGAT
                     RSFGPNTPDVPELYKEIGQPVIVGGSMETGSYLLVGTHRAHELTFGSTLHGSGRTMSR
                     TAAKKMVHGRQLQDEMKKRGIYVVTDSFSGLAEEAGFAYKDISEVVAAVEMIGISKKV
                     VRLIPIGNIKG"
     gene            complement(5936..6361)
                     /locus_tag="AC16GM_08810"
     CDS             complement(5936..6361)
                     /locus_tag="AC16GM_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR2923813.1"
                     /translation="MGYRFLDKVAIADTAFEAEAPTLPELFATCAEATFAVMVDLSTV
                     TPSEERRVELTSPSLDRLLFDWLAELIYLKDRDYFVFSRFEVKIEQNEQWRLQAVAGG
                     EKIKPEKQRLLTDVKAVTYHMFEVKKQDGGWLARVVLDT"
     gene            complement(6366..6569)
                     /locus_tag="AC16GM_08815"
     CDS             complement(6366..6569)
                     /locus_tag="AC16GM_08815"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HIR2923814.1"
                     /translation="MKEALVILAAIICVILLVQNSTLVNVKFLLWHKFLSLSVVVLTA
                     FILGAVFGYGISSRKKKQTQVKK"
     gene            complement(6581..7759)
                     /locus_tag="AC16GM_08820"
     CDS             complement(6581..7759)
                     /locus_tag="AC16GM_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2923815.1"
                     /translation="MRRRYFFWFLIFSLILVQGSWSQLPPKKLVVHKNLSGQSELFWF
                     FPDAERKELFYDDGVPQIGFYVTSAWLNNRAGVRFTPQHFPYFVDSIRIFISHDNPPS
                     PGDSASPFQVALYSDSGGLPGSPITDLVLTSADSTAWQGNGQWLPVAFGYCPPESVSF
                     WGVFHWFQATPAAPKIGADDHHDIFVSATWPSGTSQQEWDIFSQFNFMFRAVTFSNGP
                     TTADSFSVHRAADSGFVPTNTSRIATLSAPIDHFVGQPAGPESVWYYRVTAWENDQES
                     PGSNAVRIVQSPTAVEGESDFPIPQQVTLLPNYPNPFNARTVIRYALDKEAFVSLDIF
                     DLLGRRVKTLFTASQKAGLHQVAWEGTDDRGRTVPSGIYFVRLTAGAQVQRGRMLLLK
                     "
     gene            complement(7834..>8394)
                     /locus_tag="AC16GM_08825"
     CDS             complement(7834..>8394)
                     /locus_tag="AC16GM_08825"
                     /inference="COORDINATES: protein motif:HMM:NF020752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HIR2923816.1"
                     /translation="LRDILAKGYSPKAVRYLLLSVHYRQQLNFTFDGLEAARNAVERL
                     LDFKRNVADAKEAAENPKVAEQVAQTKLGFEENLDDDLNISGALGVLFDFVRDMNKII
                     ADRGLSAKDKTQVLEFLGIVDGVLGLLKTPDEELDSAVKGLIAQRDAARAQKDFTAAD
                     AIRSRLDQLGIILEDTVQGTKWKKRL"
BASE COUNT         1846 a         2490 c         2177 g         1881 t
ORIGIN      
        1 aacgacatcc ttgtgcacgg cgcccggccc ctctttttcc tcgactacct tgctaccgga
       61 aaactcgatt caacgatcgt tcagcaggtc atcaccggct tggccactgc ctgccgcacg
      121 gtggggatgg cccttttagg cggcgaaacc gcgcaaatgc ccgacttcta ccagccgggc
      181 gagtacgacc tggccggttt catcgtcgga gtggtggagc ggaagaaaat tatcgacggc
      241 aaaaaaatcg agccgggcga cctcttgctc ggcatcccct ccaacggcct ccacaccaat
      301 ggctacacgc tggcccgcaa ggcactcttt gaaatcgcgg gtttgaacgt gaacacaatc
      361 ctgcccgaat tgggcgaaaa gttaggcgac gttctcctcc gctcccaccg gtgttactgg
      421 ccggtggtga aagatttgct tcccagcgga catttgaaag gtctggccca catcaccggc
      481 ggcggtattg ccgggaatct cgttcgcatt ctgccggagg ggtgtcgagc agtcatagac
      541 cgcaagactt ggcccgtgcc gccgattttt gaagtcatcg cccgccgcgg gaatattggc
      601 aacgaggaga tgtggaaagt atttaatatg gggatcggga tgattctggt tttctccccc
      661 tcaaagaaaa aggaagtgct tgctcacttc gccaaggtca aagaaaaggt gtttgaaatc
      721 gggaggattg agaccggaaa gcggtctgta gaactattgc ccgcgtagcg cctgataaat
      781 ggtctacaaa atcctcgccg actctaccat cgccctccac ttcggctgga ttgtctttat
      841 cctcggtggc ttctttctga ccgtgcgcgc agtgtttttc cgccggcacc ggtatttttt
      901 caaccgctgg ttgcttcgct caatccattt aggaggaatt ctcttcgtcg ccaccctggg
      961 tctttttggg caagactgtc ccctgaccaa cgtggagaac gccctgcgct acaaggccga
     1021 cccgacctcg atctacaccg gctctttcat cgcccactac ctcgaacaac tggtctaccc
     1081 cggcgtaaac gtcatcgtcc tccgcgccgc cgcagcaggg attgccctct ttacgctggt
     1141 aacctacttc gccctcccgc cgtggcggaa gcacaaatag cccccagata acataaaatt
     1201 aaacgccagt gccgttttcg atacattgag agacaatggc tattgctttg tcaaagcccc
     1261 tcctggtcaa caagtaacca atcttgttgt taccctcaag gattttctct ttccataagg
     1321 agcgtaaggt gttccttacg ctcctggggc tacgcctata taacgaagaa ataatttgaa
     1381 cactggttac tgtctcggga taatgactat gcatcagtac taagatttcc tccgcaatca
     1441 ataagtcttt gagcacaatt cggcgcccac caaaatcttc aaccaagcca ccaacaggaa
     1501 cctgaatttg ctccacgagt ttgcccgctt cttccattga tcctccggac agcgatctaa
     1561 tcaactccgc aagcacccat tgggctgctg agtgaagaag tttcaggtcg tacgtattag
     1621 ggtctacatg acccttgtga agaatgttgc gcttgcttct gagcgcatac atggctctac
     1681 ctatgcgggc tgcacagatt cgaaggccat catcgaggga agtgcttcgg gattcaagag
     1741 tgcgaagata ctcgtcaacg ttgggttttg cgtcgaattt cccgacttcg agaaattgta
     1801 ggacttgtac gaacgtctcg acaaatttcc cgggagcaga ccgacctaag acgccagtgg
     1861 caacatcctg cctaagaatc aggaaattct caacgaggtc acaaacgagt tggcttggca
     1921 acgacctgcc aagtaactgg atcagtttct ttatgtccaa gcactactcc gtggagccag
     1981 gtttgttttc cagcataccc tttgccttcc gaattccctc ggttgtgagt ttgtatttct
     2041 tgcgcgcttg ctttgtagcc ccactcttat ggacttgaat tgccagtcga ggttctcgac
     2101 taatcaaatc atagagagct tttgtaatgt tgccgatggg gtatccgaga tgcttaagct
     2161 cggagttcat ctgctgggca tcaagagcct cttggctttt gacttcttgg aaccagtagc
     2221 cgaccacgag agctttttcg gcatcggttt gagggttagc cgcattatac aagctggcga
     2281 agtcctgaaa tgctgaccca ttgccattcc ccccgtttcc cttaatatca ccattccctt
     2341 ttttactccc ttccttgccg acaaagacgt tgtaacggtc ggcagcccaa cggagcactc
     2401 gcgcaatagc cttctcgtca agatcggaca gagcatcctt tatcgaagcc attgccttga
     2461 tttctgaatc gtcgttcatg attaccctcc tttgttgctc tcaacaacat taacgatccc
     2521 gtagtgcatg gtacacaata tcacatgaac taaaataagt caagtcttat gcacaagaat
     2581 ttaccgattg ctttgtattt ccggcgtcgc tggcgaacag ggtggtggtt atgtagcgtt
     2641 cgcccgtatc aggcaaaagc accataatca tttttccttt ggcctccttg cgggcggcaa
     2701 ccgaaagcgc cgcatagagt gccgcgccgg aggaaatccc ggccaaaata ccttcctctc
     2761 gcgccagccg ccgcgtgcag gcaaaggcat cctcgtcggt cacggcaatg acttcgtcta
     2821 aaatggagcg gttcaagacc tccgggataa accccacacc gatgcccggc atctggtggt
     2881 tccccgcggg cccgccggag agcaccgccg ccccggcggg ctcaaccgcc acaatgcgca
     2941 cgcccggttt gcgcgccttc agcacttcac ccaccccggt gatggttccg ccggtgccga
     3001 cagcggatac gaagacgtca accgcgccct gcgtctcctc ccagatttcc tcagccgtgg
     3061 tgcgccggtg cacctccgga ttcgcggggt tcttaaactg gtcgagcatg atcgacccgg
     3121 gaatttcttt gacgaaccgc tctgcccggg cgatggcatc acccataagg atgccgggag
     3181 tgagcaccac ttccgcgccg aggtgccgga gcagcgccac ccgttccttg gacatcgtct
     3241 ccggcatcgt caaaatgagc cggtaacccc ggatcgccgc ggcgaaggcg agcccaatac
     3301 ccgtgttgcc gccggtggcc tcgaccagag tcattccgga tcgcaggacg ccccggcgct
     3361 cggcatcctc gatcagcgcc acaccgagcc gatctttcac gctcccgcac gggttgcgca
     3421 tttctaattt cgccaccatc cggccgggca gaccctttgc caggcgtccg agttcgacca
     3481 aaggcgtccg gcccaccgtg gccgtgacat cgcgaaaaat cacctgatta tcgacgttgc
     3541 cgctgcgatt ttggaggttt gccgcacgaa aaaaatcacg acgcgcatca gcgggccaga
     3601 gtcttctcaa tgcggcggtc ggggacaaac cagatcaccg ccaccagaac gtagagcgca
     3661 caggccaaga acgagtttac gaaggccaaa ggaattgcca aaagatagat cgccaccgag
     3721 accttgcctt tgaagtcccg gcccagtgcg gtcgccagaa ccgaatccct cccgtggagg
     3781 gaaatgagag ttcgggtaag gataaagtaa gcgaccgcag ccaacaacag caccgtgcca
     3841 tagagcgcca cgggccaggg ggcgaatcga ttctggccca tccagccggt aacgaaaggg
     3901 gtgagcgaca accagaacaa aagatgcaaa ttggcccaca ggacgcggcc attaatgtgc
     3961 ttgatcgcct ggagcaggtg atggtggttg ttccagtaga tgccgagaaa aacgaaactg
     4021 aagaggtagc tcagaaagac cgggcccacg ggggagagcg cggccaaatc ggctttatgc
     4081 ggcaccgtca atcccagtac catgatggtg atgatgatgg caaacacgcc atcgctgaac
     4141 gcttccaatc tacccgtcct cattcttaat ctttctgcga atcaatctgt tgccgccgaa
     4201 ctgtttaatc cgttgtgagt tgtgtcaggt cttcagacct gacacatgcg tcgggagtaa
     4261 ctcccgacgc aactcaaatc cgttttcatc cgttgcaatc cgttgacaga ttctactcca
     4321 tccgttggga aaacccacct taatccgctt aatccgtttc aaaatccgct gtcagaatcc
     4381 gttttaatcc gttcaaaatc cgttgacagt tagcctttaa tattccctat tggaatgagg
     4441 cgtaccactt ttttcgaaat cccaatcatc tccaccgccg ccaccacctc ggaaatgtcc
     4501 ttgtaggcga accccgcctc ctcggctagg ccggagaaag aatcggtgac cacgtaaatg
     4561 ccccgctttt tcatctcatc ctgcaattgc cgtccgtgca ccatcttttt ggccgcggtg
     4621 cgggacatcg tccggcccga gccgtgcagg gtggagccga aggtcaactc gtgcgctctg
     4681 tgcgtcccga ccagaaggta cgagccggtt tccatcgagc cgcccacgat caccggctgc
     4741 ccgatctcct tgtataactc cggcacgtcg ggcgtattcg gcccgaagga ccgggtcgcc
     4801 cccttgcggt gcactaagag cggcttcttt ttgccgttga tttcgtactc ttcgatcttc
     4861 gcgatgttgt ggcagacgtc atatacgatt tccatcccca tctcgtgcgg gtcgcggcca
     4921 aagatctggg aaaaaccttc gcggatctgg tgggtgatca cctgccggtt ggcaaaggcg
     4981 gcattcgccg cgcagaccat cgccccgaaa tagttctttc cttcgggaga ggaaaaaggc
     5041 gcgcagccca attcgcggtc gattacttcg atgccgtatt tgcgcatggc ggtctcaaaa
     5101 actcgcaggt agtcggtggc cacctgatgg ccgaacccgc gcgacccgca gtgcaccatt
     5161 atgacgatct gattcgggaa ggttatgccc atccgccccg ccgtctcgcg gtcgagcaga
     5221 ttttcctctc gcacgacctg cacttcgagg tagtggttgc cggagccgag cgtccccatc
     5281 tgcgagtgcc cccgctcaaa cgccttgtcg ctcacctgat cgggatcagc gccttcgatg
     5341 gcgccggatt cctcgatgcg ttctgcgtcc tccggccagc cgtatcccat cttgacgatc
     5401 ttctgcgcgc cgtgcaccat cacctcccga aactcctgcc tggagaggcg cagggaactt
     5461 ttcgagccga cgccggaagg aacgagccgg aaaagtgtgt gcatcagttg ttccaatttc
     5521 ggcttgactt catcgtagtt gagatttgtc cgcaccagcc gcatcccgca gttgatgtca
     5581 aacccgatcc cgcctggcga aatgacgcct tccttgagat cgaatgccgc cactccgccg
     5641 atggggaagc cgtaccccca gtggccgtcc ggcatgcaga tcgaaacttt ctggatgccg
     5701 ggcaaacagg cgacgttggt cacctggttg tagaccccct gatccatcgc gttgaccaat
     5761 gcgtccgtcc ccagaatcca ggccggcacc cgcatccccg gtttttgatc cggcgcaatt
     5821 tcccagatcc ccggcgctat ctgctttttt tccactatct cctcacctct atgaagtgtc
     5881 tttggggttg ctctttcccc ggactcctaa ctcccgaatc cgaaatcctg tcttttcagg
     5941 tgtccaggac cacccgcgcc agccagcccc cgtcctgctt tttgacctca aacatatggt
     6001 acgtcactgc tttgacatcg gtcaacagcc gctgcttttc gggtttgatc ttttcccccc
     6061 ctgcgacggc ttgtaaacgc cactgctcat tctgctcaat tttgacttcg aaacgtgaaa
     6121 agacgaagta atcgcgatct ttgaggtaga tcaactccgc cagccagtcg aagagcagcc
     6181 gatctaagga gggcgaggtc aattcgaccc ggcgttcctc agacggcgtc acggtgctca
     6241 aatcgaccat caccgcaaag gtcgcctccg cgcaggtggc aaaaagctcc ggcagcgtgg
     6301 gggcctccgc ctcaaaagcc gtgtcggcga ttgcgacctt atctaagaag cggtagccca
     6361 taccgctact tcttgacttg tgtttgcttc tttttacgtg acgaaattcc gtagccgaag
     6421 acggcgccca aaatgaaagc cgtcaacacc accacggaaa gcgagagaaa tttatgccag
     6481 aggagaaact tgacgtttac cagcgtgctg ttttgaacca aaagaatcac gcagatgatg
     6541 gcggccagga tgacaagggc ttctttcatt gtcgggttga ctatttcagg agcagcattc
     6601 tgcccctctg cacctgcgcc ccggcggtta accggacgaa gtagattccc gaaggtacgg
     6661 ttcgcccccg gtcatcagtt ccttcccagg cgacctggtg caaccctgcc ttctggctgg
     6721 cggtgaaaag cgttttcact ctgcggccca aaaggtcaaa aatatccagc gagacgaacg
     6781 cttctttatc cagcgcatac cggatcaccg tgcgggcatt gaacgggttg gggtagttgg
     6841 ggaggagcgt aacttgttgc gggatgggaa aatctgactc accttctacg gccgtcgggg
     6901 attggacgat gcgcaccgcg ttcgaacccg gactctcctg gtcattctcc cacgccgtca
     6961 cccggtagta ccagaccgat tccggcccgg cgggttgacc cacgaagtga tctatcggag
     7021 cagacagggt tgcgatccgg ctggtgttcg ttggaacaaa accggaatct gccgcccgat
     7081 gaaccgaaaa actatcggct gttgttgggc cattggaaaa tgtgacggca cgaaacataa
     7141 agttaaactg agagaaaatg tcccattcct gctgagaagt gcccgatggc cacgtcgccg
     7201 aaacgaagat gtcgtggtgg tcgtcggccc caattttggg cgccgcgggc gtggcctgaa
     7261 accagtggaa caccccccag aaggaaaccg attcgggtgg gcagtacccg aaagcgaccg
     7321 gaagccactg accgttcccc tgccaagcgg tactgtccgc acttgtgagc accagatcgg
     7381 tgatcggcga cccgggtaat ccccctgaat cggagtagag tgctacctga aaaggcgagg
     7441 cagaatcccc cggggatggc ggattgtcgt gactgatgaa aatgcgaatc gaatctacaa
     7501 agtaaggaaa atgctgcggc gtaaagcgca ctcccgcccg gttgttcagc caggccgagg
     7561 tgacgtagaa accgatctgg ggcacgccgt catcgtagaa taactccttc cgctctgcat
     7621 ccgggaaaaa ccagaacagt tccgactggc cggaaagatt cttgtgcacc acaagttttt
     7681 tggggggaag ttgtgaccag gaaccctgaa ccagaatcaa tgagaagatg agaaaccaaa
     7741 aaaagtaccg gcgacgcatt gacgcctcct ccgaccggaa ctatcgagtt tgtaggtgat
     7801 taaacgtttt ggaaaagaaa agtcaagccg gccctacaac cgcttcttcc acttggtgcc
     7861 ctgcaccgtg tcttccagaa tgatgcctaa ctggtccagc cggctacgga tggcatccgc
     7921 ggctgtgaag tctttctgcg cgcgggctgc gtcccgctgg gcaatgagtc ccttgaccgc
     7981 ggaatcgagt tcctcgtccg gcgtcttaag gagtccaagc acgccgtcaa ctatccccaa
     8041 aaattccagc acctgcgttt tgtctttcgc ggaaagacct cggtcggcga ttattttatt
     8101 catgtcccgc acgaaatcga acagcacccc cagtgccccg gaaatgttca aatcgtcatc
     8161 gagattctcc tcgaatccta gcttggtctg ggctacctgt tcagcaactt ttggattttc
     8221 tgccgcttct tttgcatcag ccacgttgcg tttgaagtct aagagccgct ccaccgcatt
     8281 ccgcgccgct tccaggccgt cgaaggtgaa atttaactgc tgccggtaat ggacggagag
     8341 caaaaggtac cgcaccgctt tgggagagta ccccttggcc aggatgtcgc gcag
//
LOCUS       DBNGZD010000330         7267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_191916, whole genome shotgun sequence.
ACCESSION   DBNGZD010000330 DBNGZD010000000
VERSION     DBNGZD010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_191916"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="AC16GM_08830"
     CDS             <1..277
                     /locus_tag="AC16GM_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2923817.1"
                     /translation="AAKAGLIGFTKACARELAPFGITVNAVAPGFIETEMTQKLPEKV
                     REGLVGLIPLGRTGAPEEVAGVVAFLASDEARYLTGQVVQVDGGLLM"
     gene            340..591
                     /gene="acpP"
                     /locus_tag="AC16GM_08835"
     CDS             340..591
                     /gene="acpP"
                     /locus_tag="AC16GM_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR2923818.1"
                     /translation="MASVEQKVREIIVEQLGVDPEQVTREASFVEDLGADSLDTVELV
                     MALEEEFDLEIQDEEAEKLATVGDAIEYIEGRLEQQEAT"
     gene            619..1869
                     /gene="fabF"
                     /locus_tag="AC16GM_08840"
     CDS             619..1869
                     /gene="fabF"
                     /locus_tag="AC16GM_08840"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR2923819.1"
                     /translation="MEKRRVVVTGLGVLSPVGNTVEEFWQALVAGTSGVAAVTRFDCS
                     AFPTKIAGEVKGFDSLRYMDKKEQRRADLTEQYAIAASEMAVQDAGLDVEKLDRDRAG
                     VVIGSGIGGIGTFEKQFTILLQNGPDRVSPFFIPMMIIDMCPGLVSMRYGFKGPNYAT
                     VSACASSAHAIGDAFKIIQRGDADLMLTGGCEATITPASMAGFCSAKAMSTRNDAPEK
                     ASRPFDRGRDGFVMGEGAGMLVLESLDHARSRGVKMYAEIVGFGMTADAYHITAPAPN
                     GEGAARAMRAALTDGGLKPEEVDYINTHGTATEAGDIAETQAVKAVFGAQAQKLVLNS
                     TKSMLGHLLGAAGAVELIAAIKSLETGIIHPTINLDEPDPQCDLDYTPHRSRQKTVRV
                     ALSNSFGFGGHNVTLAVRSFNGRP"
     gene            1926..2720
                     /gene="rnc"
                     /locus_tag="AC16GM_08845"
     CDS             1926..2720
                     /gene="rnc"
                     /locus_tag="AC16GM_08845"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR2923820.1"
                     /translation="MRVRFLELFRSVWKRENLDEAPLAAVERLFGYAFRERSLLATAL
                     THRSSTKGQTEQRNGGAAARETSYERLEFLGDAVLGLLISEYLYHQYPDQDEGELTKL
                     KAALVNEVSLSRVARAIGLGNHLFLSSEGDKSGTRQRSSVLADAYEAVVGAIYVDGGL
                     APVRRVIARQMLSLLDDLLYDISHFNFKGELLEFLQARALGMPRYQVVSERGPDHAKE
                     FTIAVSVQGETLGTGTGSTKKEAEQKAAAAALEKLSGQKATSLEAL"
     gene            2742..3512
                     /locus_tag="AC16GM_08850"
     CDS             2742..3512
                     /locus_tag="AC16GM_08850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR2923821.1"
                     /translation="MHVIVCIKQVPDIAGIRVEDNKVVPPPGSGRMNPFDEYAIEEGI
                     RLKERFGGTVTAVSVGDATAELALREAVALGVDNAVLLTDPQFGGVDPLTTACILAAG
                     MRKIGAFDLLVCGKQAVDDDTALVPGALAGFLRLPQVIFVKKIETIKDSKAVVQRMTE
                     EGYEVVETQLPAVVSVVKEINEPRLPSLKGKMRAKSAPVTKYSAADLGLTDEILAPKM
                     RVVKVSPPPARTKGEMLEGTPEEIAENLFRKLREAQVI"
     gene            3846..5573
                     /locus_tag="AC16GM_08855"
     CDS             3846..5573
                     /locus_tag="AC16GM_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR2923822.1"
                     /translation="MRTVARFLLGVFVFTVVLGFSAQAQMSGSISGNVVDDSTSAPIA
                     GARVVAFWVDSILPFPAGSAMTDPAGNYQIMHLRPGDYLVLACAMAYECEFYDNVHRP
                     DQATRVHVLMNQNTGGINFALSHIGPPPPPPSIGTIAGQVTDAVTGNPIPGAMVVAMG
                     RNPLFMRPGGFGTRTDSGGNYILPVPPDTYEVKAFAMGYQPGVYPNPVVVGPDQHVTC
                     IDIQLQPVGAGAILGRVTDRVTGLPLEHALVIAHRIDGFGFGQAPTDANGDYTINRLL
                     AGTYRVVALARGYFPAAFPDPVSVIENQTTTGIDFALLPFTPPPAGHIAGRVTDDSTG
                     LPIADAVVGAVAFDSTHRMPIIRFTHTGEDGSYRIESLPSIPFFVIAWAHGYLAELYD
                     NAHRFEDATRVIPPADGIDFALTLKPMGPPAVSGIVMNQNLGTPLAGVLVNAVTPQGE
                     VAGSAVSLPDGSFLVEGLGPGQFTLEARSNLAPPVAVGPFDLSGDGQSGIVVNVPAAV
                     ANVRGDVNRDGLIDVVDIVGEIDAVVFGRLLDPLLADVNCDGVVDIVDIVGLVQFVVF
                     GRPSPCALN"
     gene            5791..7206
                     /gene="lpdA"
                     /locus_tag="AC16GM_08860"
     CDS             5791..7206
                     /gene="lpdA"
                     /locus_tag="AC16GM_08860"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR2923823.1"
                     /translation="MPEISCDIFVIGSGPGGYVAAIRAAQLGNKVAIAEKDSWGGVCL
                     NWGCIPTKALLFTSELFHLIEIAKEYGIQTSVSGFSLDTLRAKKERVVKQLVGGVEFL
                     LKGNQVTALKGTARFLNPRQIEVSSDGQKSLVTARKTIIATGTEPVKLPMLPVDGDLV
                     IDSQDALELKRIPKKIVIIGGGAIGIEFADIYRAFGSQVVVVEMLDSILPTLDREVAG
                     TLANILRKRGIEIYTSHKVTQTRLEKNKAILTVEPVAGAAPPLSIECDMVLVAVGLRG
                     QVSGFGLEQTGVLADKHGFITVNGRLETNVPDLYAIGDVVGGKLLAHKASHEGIIAAE
                     AASGRKVEMDYRVVPYAVFTDPEIGAVGLTEEEARAQGYNLKIGRFPFRVLGKAIGIS
                     RTEGFVKIIGDAETDELLGMHIIGAHAGDLIAEGAMAMAMSATCEDVASTIHVHPTLA
                     EAVMEAALDADQRVIHLISKK"
BASE COUNT         1475 a         2059 c         2253 g         1480 t
ORIGIN      
        1 cgcggcgaaa gccggcctga tcggcttcac caaagcctgc gcgcgggaac tggcaccttt
       61 cggcatcacc gtgaacgccg tggcgccggg gttcatcgag acggaaatga ctcagaaact
      121 gccggaaaaa gttcgggagg ggttggtcgg tctcattccg ctgggccgta ccggcgcgcc
      181 ggaagaagta gcgggggtgg tggctttcct ggcctccgat gaagcccgtt acctgaccgg
      241 ccaggtcgtt caggtggacg gcggcctgtt gatgtagacg gtttggtcac gtatataaac
      301 ggcacagcgg tacgattcag aaaacaagga ggcaaacgga tggcatcggt tgagcagaag
      361 gtgcgtgaaa tcattgtgga gcagttaggc gttgacccgg aacaggtgac ccgggaagcc
      421 tcgttcgtgg aagacctggg agccgactca ctggataccg tcgaactggt catggcgctc
      481 gaggaggagt tcgacttgga aatccaggat gaagaagccg aaaagctcgc caccgtgggc
      541 gacgccattg agtacatcga agggcgcttg gaacagcagg aagcgacgta gctttcttcg
      601 gcagctcaag cgagcactat ggagaaacgt cgggtggtag tcaccggttt gggggtgctc
      661 tcgccggtgg gcaataccgt cgaggagttc tggcaggcgc tggtggctgg cacgtcgggt
      721 gtggctgccg tcacccgctt tgactgttcg gctttcccca ccaaaatcgc gggcgaagtc
      781 aagggttttg attccctccg ctacatggac aaaaaggagc aaaggcgagc cgacctgacc
      841 gagcagtacg ccattgccgc aagcgaaatg gccgtccagg atgccgggct ggatgtggag
      901 aaactcgacc gggaccgggc tggcgtcgtc atcggctccg ggatcggcgg gatcggtacg
      961 ttcgaaaaac agttcacgat tctcctccag aacgggccgg accgggtgtc gccctttttc
     1021 attccgatga tgatcatcga catgtgcccc ggactggtct ccatgcgcta cggtttcaaa
     1081 gggcccaatt acgccacggt gtcggcctgc gcctccagcg cgcacgccat cggcgatgcg
     1141 ttcaagatca tccagcgcgg tgatgccgat ctcatgctca ccggcggctg cgaagccacg
     1201 attacccccg cctccatggc ggggttctgc tcggccaagg cgatgtccac ccgcaacgac
     1261 gctcccgaga aagcgtcccg cccgttcgac cgaggccggg atggattcgt catgggagag
     1321 ggggccggta tgctcgtctt ggaaagtctc gaccatgccc ggagccgcgg tgtcaaaatg
     1381 tatgcggaga tcgttggttt cgggatgacc gccgacgcct accacatcac cgcaccggcg
     1441 ccgaacgggg aaggggcggc gcgggccatg cgggcggccc tgacggatgg cgggctcaaa
     1501 ccggaagagg tggactacat caacacccac ggcaccgcta cggaggcggg tgacatcgcc
     1561 gaaactcagg cggtgaaggc ggtgttcggt gcccaggccc aaaaattagt tttgaactcc
     1621 accaagtcga tgctcgggca cctcctgggt gccgccggag cggtggaatt aatcgccgcc
     1681 ataaaatcgc tggaaacggg aatcatccac cccacgatca acttggacga gccggacccc
     1741 cagtgtgacc tcgactacac accgcatcgg tcgcgccaaa agacggtacg ggtggcgctc
     1801 tccaattcct tcggcttcgg cggacacaac gtcaccctag ccgtccgcag cttcaacggc
     1861 cgaccgtaat ctcccctttt ttcgctggta aaatcaccgg attttggcta catttacgga
     1921 agcgtatgcg ggtgcggttc cttgaactgt tcagaagtgt ttggaaacgg gaaaacctgg
     1981 acgaagcgcc gttggctgcg gtggagcggc tcttcggtta cgccttccgc gagcggtccc
     2041 ttttggccac ggcgctcacc caccgctctt ccaccaaagg ccaaactgag cagcgcaatg
     2101 gaggggcggc ggcgcgggag acttcctacg aacggctgga atttctgggt gatgccgtgt
     2161 tgggtctgct gatttccgag tacctttacc atcaatatcc ggaccaagat gaaggagaac
     2221 tcaccaaact caaggcggcc ttggtcaacg aagtgagcct gtcgcgggtg gcgcgggcga
     2281 tcgggttggg taaccatctt ttcctctcgt ccgaaggaga caaaagcggc acccggcagc
     2341 gcagttccgt tctggccgac gcgtatgaag cggtcgtcgg ggcgatctat gtcgacggcg
     2401 ggttggcacc ggtacgccgg gtcatcgccc gtcagatgct ctccttgctg gacgacctcc
     2461 tctacgacat cagccatttc aatttcaagg gcgaactgct ggagttcctc caggcgcggg
     2521 ctttggggat gccgcgctac caggtggtct ccgagcgcgg tcccgatcac gccaaggaat
     2581 tcaccattgc cgtttcagtc cagggtgaaa ctctgggaac cggcaccggc tcgaccaaaa
     2641 aggaagcgga acagaaagcc gccgccgccg cgctggagaa attaagcggc cagaaggcca
     2701 cctctctgga agcactctga ccaaatttga gggacacccg catgcacgtc attgtctgca
     2761 tcaaacaggt tccggatatc gcggggattc gcgtcgaaga caataaggtg gtacctcccc
     2821 cgggttccgg gcggatgaat ccgttcgatg agtacgcaat agaagaaggt atccgactga
     2881 aggaacgttt cggcggcacg gtgacggctg tgtcagtggg cgacgccacc gccgagctgg
     2941 ctttgcgcga ggccgtggct ttgggcgtgg ataatgccgt gctgcttacc gatccccaat
     3001 ttgggggtgt cgatccgctc accacggcgt gcatcttggc cgccggcatg cggaagatcg
     3061 gcgcttttga tctccttgtc tgcggcaagc aggcggtgga tgacgacacc gcgctcgtgc
     3121 ccggtgctct ggccggcttt ctacgtctgc cgcaggtgat ctttgtcaaa aagatagaga
     3181 cgatcaagga cagtaaggcc gtggtgcagc ggatgactga agagggatac gaagtggtgg
     3241 aaacgcaact gccggcggtc gtttccgtgg tgaaagagat taacgagccg cggttgcctt
     3301 cgctgaaggg caaaatgcgc gccaagagcg cgccggtaac caaatactcg gcagctgact
     3361 tgggcctgac cgacgagatc cttgccccca agatgagagt ggtaaaggtt tctcccccgc
     3421 cagcccgcac caaaggggaa atgctggagg ggacaccgga agagatcgct gaaaacctgt
     3481 tccgtaaact gcgcgaagcg caagtgattt aactccccat ctcctttacg ttcatagtct
     3541 tgcagcgacg gtctccggac cgtcggagcc caatttaggc aagagatttt gtgatgtggg
     3601 tatacctgca gtcattttca gactcttctt gtccccaata gtcaaaaaat atcttgcttt
     3661 tgccgcaggc cgggttatat tttttgtaac ttttattcga ttttcttgtc ctacggatgg
     3721 gagaatcgga gtcgaaagag ggcagttgct gtagtccgga tcgggtttgc ggcagtgctg
     3781 agtagaaaag ttggttcggt ggtggatagt ttcctcaaac aatccctaca attcaaggag
     3841 ggtgtatgcg taccgtagct cggttccttc tgggcgtttt cgttttcacg gtcgttctgg
     3901 ggttctccgc tcaggcccag atgagcggtt caatttccgg caacgtggtt gacgattcaa
     3961 ccagtgcacc gattgccggg gcgcgggtgg tggccttctg ggttgacagt attctccctt
     4021 tcccggcagg ttcagccatg accgacccgg ccgggaatta ccagatcatg catctgcggc
     4081 cgggtgacta cctcgtctta gcctgcgcca tggcgtatga atgcgagttt tacgacaacg
     4141 tgcatcgccc cgaccaggca acacgggttc atgtccttat gaaccagaac acgggcggta
     4201 tcaactttgc gctctcccat atcggtccac cgccgccgcc tccttccatc ggcactatcg
     4261 ccgggcaggt aaccgacgcg gtaaccggta atccgattcc cggagcaatg gtggttgcca
     4321 tggggcggaa tccgcttttc atgcgtccgg gagggttcgg aacgcgaaca gactccggcg
     4381 gcaattacat tctgccggta ccccccgata cctatgaagt caaggccttc gccatggggt
     4441 accagcccgg ggtttacccg aatccggtgg tcgtgggacc cgaccagcac gtgacgtgca
     4501 tcgacattca gttgcagccg gtgggtgcgg gggcgatttt gggtcgcgtg actgatcgcg
     4561 tgaccggcct gcccctggaa cacgcgctgg tgatcgcaca tcgcatagac ggtttcggtt
     4621 ttgggcaggc gccgactgat gccaatggtg attacaccat caaccggctg ctggccggca
     4681 cctatcgggt ggtggcgttg gcgcggggtt acttcccggc ggcgtttcct gatcctgttt
     4741 ccgtgatcga aaatcaaact accaccggca ttgacttcgc gctcctgccg ttcacaccgc
     4801 cgcccgcggg tcacattgcc gggcgggtaa ctgacgacag caccggcttg ccgattgcgg
     4861 atgcggtggt tggtgcagtc gccttcgact ctacgcaccg gatgccgatc atccggttca
     4921 cgcacaccgg agaggatggc tcctaccgga ttgagtcctt gccttccatt ccgtttttcg
     4981 tgatcgcctg ggcgcacggc tacctggccg aactctacga caatgcccac cgctttgaag
     5041 acgccacccg ggttatcccc ccggccgacg gaattgactt tgccctaacg ctcaaaccta
     5101 tgggcccgcc ggcggtctcg ggcatcgtca tgaaccagaa cctgggtaca cccctggcag
     5161 gcgttctggt gaatgccgtg acgcctcagg gtgaggtcgc tggttcggcc gtctccctac
     5221 cggatggcag tttcctggtg gagggccttg gccccggtca gtttacgctc gaagcccgct
     5281 ctaacctggc gccgccagtg gctgttggtc cttttgacct ctccggcgac ggtcagagcg
     5341 ggatagtggt gaacgtcccg gctgccgtgg ccaacgtgcg cggtgacgtc aaccgtgacg
     5401 ggctgatcga cgtggtggac atcgtgggcg aaatcgacgc agtcgtcttc gggcggctcc
     5461 tcgatccgct tttggccgac gtcaactgcg acggagtcgt ggatatcgtc gacatcgtcg
     5521 ggctggtgca gttcgtggtt tttggccgcc cgagcccctg tgcgctcaac tgacgtttga
     5581 ccgttcgaga aaagccccgc ttcaaggcgg ggcttttcta ttttggggaa tagaaccgcc
     5641 gcttcggtct tcggcaagct cgggcgaagg agaaagcgtt acagccggta tactcacacg
     5701 ggagaggaat tctcggacat tgggcgggca aaattgacaa gagccggttg tgattctata
     5761 ctcagccgga caatagagaa aggataggcc atgcctgaaa tttcctgtga catttttgtg
     5821 atcggttccg gcccgggcgg ctacgtggca gccatccgcg ccgctcagtt ggggaacaaa
     5881 gtggcgattg ccgaaaaaga ttcctggggc ggggtctgct tgaactgggg ctgcatcccc
     5941 accaaggcgc ttctgttcac gtccgaactc ttccatttaa tcgagattgc caaagagtac
     6001 ggcattcaga ccagtgtctc cggattttcc ttagataccc tccgtgcgaa gaaagaaaga
     6061 gtggtcaagc agctggtggg tggagttgag tttctgttga aagggaatca ggtcaccgcg
     6121 ctcaaaggaa cggcccgatt tctcaatccc cggcaaattg aagtctcaag tgatggacag
     6181 aaatctctcg taactgctcg gaagaccatc atcgccaccg gtaccgaacc ggtgaagctc
     6241 cccatgctgc cagtggacgg cgatctggtg atcgacagcc aggatgcgct ggaactgaaa
     6301 cggataccca aaaaaatcgt catcatcggg ggcggcgcga tcggcattga attcgccgac
     6361 atctaccggg ccttcggcag ccaggtggtc gtggtcgaaa tgctggactc gattctgccg
     6421 accttggacc gggaagtggc cgggactttg gcgaacatcc tgcgcaagcg gggaatcgaa
     6481 atttatacca gccacaaagt cacccagacg agactggaga agaataaagc gattttgacc
     6541 gtcgaaccgg tcgccggggc cgcaccacca ctttcgatcg agtgcgatat ggtgctggtg
     6601 gcggtcggct tacgagggca ggtctccggt ttcggtttag aacaaaccgg cgtgctggcc
     6661 gacaagcatg gtttcatcac cgtcaacggc cggctggaaa cgaacgtccc ggacctctac
     6721 gccatcggcg acgtggtcgg cgggaaactt ttggcccaca aggcttcgca cgaggggatt
     6781 attgccgccg aagccgcttc cggccggaaa gtcgaaatgg actaccgggt cgtcccatac
     6841 gccgtcttca ccgatccgga aatcggggcg gtcggcttga ccgaagagga agcgcgcgcg
     6901 caggggtaca acctaaaaat cgggcgcttc cccttccggg tgctgggaaa agcgatcggc
     6961 atctcgcgca ccgaggggtt cgtaaaaatt atcggcgatg ctgaaaccga cgaactcctg
     7021 ggtatgcaca tcatcggggc gcacgcgggc gatttaatcg ccgaaggagc aatggcgatg
     7081 gcaatgagcg ccacctgcga ggacgtggct tcaacgatcc acgtccaccc cactctggcc
     7141 gaagcggtga tggaggcggc cctggacgcc gaccagcggg tgattcatct gataagcaaa
     7201 aagtaaaagc cggcgacctg aataccccag tacgtttttc cgtaattagc ttatcgattt
     7261 ctcccgt
//
LOCUS       DBNGZD010000331         3111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1373184, whole genome shotgun sequence.
ACCESSION   DBNGZD010000331 DBNGZD010000000
VERSION     DBNGZD010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1373184"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..956)
                     /gene="murQ"
                     /locus_tag="AC16GM_08865"
     CDS             complement(24..956)
                     /gene="murQ"
                     /locus_tag="AC16GM_08865"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR2923824.1"
                     /translation="MSRKVFNEIKDLVTEAFNPRTKKLDRLTTVAILQRINREDRTVP
                     SAVAKEIPSIARAVELYVAALGSGGRVFYVGAGTSGRLGVLDAAECPPTFGTDPKKIQ
                     GVIAGGFRTLIRSREGVEDRRQAGAKDLRRRKLTKRDIVVGITASRRTPYVLGALAYA
                     RRVGARTVFICANPRRMVKVKANIVICLVVGPEVIAGSTRMKAGTAQKLVLNMLSTAA
                     MVRLGKVYGNVMVDLRATSEKLVERSKRILMLLGKISYPQAEKLLRAAGGSVKVALVM
                     RLTGLSRREAEKRLAKAGGFVYKVVAEQKKKSAR"
     gene            complement(1061..1849)
                     /locus_tag="AC16GM_08870"
     CDS             complement(1061..1849)
                     /locus_tag="AC16GM_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923825.1"
                     /translation="MKTLKQILAALTLGLALVVILGLRCHRIAGPGGTPQPTQVYRID
                     GVAVRDMNSTDQVWLRVRRSGALFDSATVLFDTVAVPRIASGEYQRLSPGVNLTPGNH
                     TLAVRYAPDNLNLSLALVMPDTFVIDSSSLDATERQYRGIPATVTLDWSPSVNAQGYL
                     VAANRETPVRDNFQSFLQLSVGANSFGFPRNTWRDTLADTVLTGLYRIHVASYRPTLV
                     SYPGMPFVLPALGLSDNINSPNLTGRFGASVIAPFDTIRVITQF"
     gene            complement(1964..>3111)
                     /locus_tag="AC16GM_08875"
     CDS             complement(1964..>3111)
                     /locus_tag="AC16GM_08875"
                     /inference="COORDINATES: protein motif:HMM:NF025418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HIR2923826.1"
                     /translation="YHDGEKFSVLGGFFFDQQLAREPAYPGKVWRGWAGDIDRATAHF
                     ALPWFFLSLGRDRLHWGPGRSGSLILSRTSPSLDFLALSGQYGWISFVSFAGVLDALG
                     VNRSGLPAPDSTIFYNRYLSGHRVELFLWRKLALGFSETVVYGGQGRTLEPYYLNPLF
                     WFQGAQLNANRDDNTFAAIDFSFYPVRTLELYGQLLLDDFQIEKKTQSDQEPNEIGYQ
                     TGFFLADPFSLTGDFNLEYTRVANRTYNQALPYNRYLNRNQSLGYPSGPDGEHFFVSF
                     RKWLPGAQALTGTYQYHRQGEGKIAAPWTTPWLSVSGAYHEPFPSGVVERTDEVSLLW
                     QYAYRRNVRLEIETGFRQVKNLAHQAGQTERGWLVGLGLQIDGVWTK"
BASE COUNT          642 a          906 c          930 g          633 t
ORIGIN      
        1 caaatacctt tactgaactc accctaacgt gccgacttct ttttctgctc cgcaactact
       61 ttatacacaa agcccccggc tttggcaagc cgcttttccg cttcccggcg cgaaagaccg
      121 gtcaagcgca taacgagggc aactttcacg cttccgcccg ccgcgcgcaa aagtttttcc
      181 gcctgggggt acgaaatttt tcccagaagc attaagattc tcttcgaccg ctcgactaat
      241 ttctcggaag tcgcccgcag atcgaccatc acattcccgt agactttgcc caagcggacc
      301 atcgctgcgg tcgagagcat attgagcacc aacttctgcg cggtgccggc tttcattcgc
      361 gtggagccgg caatcacctc cggccctacc accaagcaga tgacaatgtt ggctttcacc
      421 ttgaccattc tccgggggtt ggcgcagatg aaaacggttc gagcaccgac gcggcgggcg
      481 taggcgagcg cacctaaaac gtacggcgtc cgccgcgagg cggtgatgcc cacgactata
      541 tcacgtttgg tcagttttct cctgcggaga tccttcgctc ccgcttgccg cctgtcttcc
      601 actccctcgc gcgagcgaat gagggtgcgg aaacctccgg caatgactcc ctgaattttt
      661 ttcggatcgg tgccaaaagt cggcgggcac tcggcggcat ctaacacgcc gagacggccg
      721 gaggtgcctg cgccgacgta aaacacccgc ccgccggagc ccagcgccgc tacgtagagt
      781 tcaaccgctc tggctatgga aggaatttct ttcgcgacgg cggagggaac ggtgcgatct
      841 tcacggttaa tccgctggag gatggcaacg gttgtgagac ggtctaattt tttggttcgg
      901 ggattgaatg cctcggtgac gaggtctttg atttcgttga aaacttttcg ggacaggggc
      961 gactcctctt cgcaccgcct gaggcgaggt cactcactcg atttaccgga ccaccacaag
     1021 tctcgggtga tgttacagaa tccgctttgc gtgcgcaagc tcaaaactgg gtgatcaccc
     1081 ggatggtatc gaacggcgca attaccgacg ccccgaagcg gcccgtcagg ttggggctgt
     1141 tgatgttgtc gctcaaaccc agtgccggca ggacgaacgg catcccgggg tacgacacta
     1201 aagtcgggcg ataggaggcc acgtggatgc ggtagaggcc ggtgagaacc gtatcggcca
     1261 gcgtatcccg ccacgtattg cggggaaagc cgaaactgtt ggcgcctacc gagagctgga
     1321 ggaaactctg aaagttatcc cgcaccggcg tctcccggtt ggcggcaacc aggtatcctt
     1381 gagcgttcac cgagggcgac cagtccaggg tcacggtcgc cgggatgccc cggtattgcc
     1441 gctccgtagc gtccagggat gaactgtcga tcacgaacgt atcgggcatc accagtgcca
     1501 gtgagagatt gagattatcc ggagcatagc gcaccgcaag ggtgtggtta cccggagtca
     1561 ggtttactcc cggggaaagg cgctggtatt cgccggaggc gattcgcggg acggcgaccg
     1621 tgtcgaagag aaccgtcgcg ctgtcaaaaa gggcgccgct gcgccgcacc cgcagccaca
     1681 cttgatcggt gctgttcata tctcgtacgg ccacgccgtc tatccggtaa acctgcgtcg
     1741 gctggggcgt gccacccggc ccggcaatcc ggtgacaccg caggccgagg atgaccacaa
     1801 gcgcaaggcc gagggtaaga gcggccagaa tctgctttag agttttcatc ttttcttctt
     1861 tccttgagtg cggttgtctc cgtactgttt tatctctata tcggctttct ggaacacttt
     1921 attgatatga tctgcttgcg gtgctggagt ccgcacaagc cggctacttc gtccaaacgc
     1981 cgtcaatttg cagccccagc cccaccagcc atccgcgttc ggtctggccc gcctggtgtg
     2041 ccagattttt gacctgccgg aatccggtct cgatttccag ccggacgttc cgccggtagg
     2101 catactgcca taaaagcgat acttcgtcgg tgcgctccac cactcccgac gggaacggtt
     2161 cgtggtacgc gccgctgacc gaaagccacg gcgtcgtcca gggggcggca attttccctt
     2221 ccccctgccg gtggtactgg taggttccgg tgagcgcctg cgctccgggc agccacttgc
     2281 ggaaagaaac aaaaaaatgc tcgccatccg gcccggaggg ataccccaga ctttgattcc
     2341 ggttgaggta gcgattgtag ggcaatgcct gattgtacgt ccggttggca acgcgggtgt
     2401 actccaggtt gaaatcacct gtcagggaaa aaggatcggc caggaagaac ccggtttggt
     2461 agccgatttc attgggttct tgatcgcttt gcgtcttctt ttcgatctgg aaatcgtcga
     2521 gcaaaagctg gccgtacagt tccagtgttc gtaccggata gaacgaaaaa tcgatagcag
     2581 cgaaggtgtt gtcgtcgcgg ttggcgttca actgtgctcc ctggaaccag aagagcgggt
     2641 tgaggtagta cggctccaga gtccgtccct ggccgccgta gacgacggtc tcggaaaagc
     2701 cgagggccag tttccgccag agaaaaagtt ccacccggtg gccggagagg tagcggttgt
     2761 agaagatggt cgaatcggga gccggaagac cggaacgatt tactcctagt gcatcgagga
     2821 ctccggcaaa agaaacgaag gaaatccagc cgtactgacc tgagagggca aggaaatcga
     2881 gagacggcga cgtgcgcgaa aggatgagac tccctgaccg ccccggcccc cagtgcagcc
     2941 ggtcgcgccc gagcgagagg aaaaaccagg gcagggcgaa gtgcgcggta gcccgatcaa
     3001 tatctccggc ccagccccgc cagaccttgc cggggtaggc gggctcgcga gccagttgct
     3061 gatcgaagaa aaatccgccg aggacggaga atttctcccc gtcgtggtag g
//
LOCUS       DBNGZD010000332         7128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_540723, whole genome shotgun sequence.
ACCESSION   DBNGZD010000332 DBNGZD010000000
VERSION     DBNGZD010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_540723"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1862
                     /locus_tag="AC16GM_08880"
     CDS             339..1862
                     /locus_tag="AC16GM_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2923827.1"
                     /translation="MSGFARFLSSLCSGCLILFSSFSSALSQASLIYQKNGADTTAGF
                     GFSIASAGDVDGDGKADFIVGAPQSGTPYTSAGTGSVFVYSGATGILLHQKNGSVLFD
                     NFGHSVAGAGDVNGDGKADFIVGAPFAAPAGVEQAGAVYLYSGGTGSLLFQKNGIDPF
                     DWFGLTVAGVGDVNGDGRADFIVGAPLADPGGVVNAGSIFVYSGATGTLLYRKDGAAF
                     LDNFGRSVAGAGDIDGDGRADFIVGALLADPGGVEAAGSAFVYSGATGSLLYQKDGAD
                     SVDLFGRSVAGAGDMDGDGRADFIVGAPDANPGGVRDAGTAYVYSGATGDLLFQKNGT
                     DSADLFGFSVAGAGDVDGDGKADFIIGALLADPGGLTDAGSAYVFSGATGALLFSVNG
                     TAPLDELGHQVASAGDVQGDGKAEVIVGAPETDPEGIIDAGSAFVYGFCVLRGDVDNS
                     GVADIIDIVGLVRNVVFGDPLPNTVAGDTDCNGIWDISDLVLLVQYVAFGTPTPCCL"
     gene            complement(1890..1963)
                     /locus_tag="AC16GM_08885"
     tRNA            complement(1890..1963)
                     /locus_tag="AC16GM_08885"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1927..1929),aa:His,seq:gtg)
     gene            complement(2131..2204)
                     /locus_tag="AC16GM_08890"
     tRNA            complement(2131..2204)
                     /locus_tag="AC16GM_08890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2168..2170),aa:Arg,seq:tct)
     gene            complement(2261..2650)
                     /locus_tag="AC16GM_08895"
     CDS             complement(2261..2650)
                     /locus_tag="AC16GM_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2923828.1"
                     /translation="MEKQSKAVVQSYRDLVVWQKAHELTLSVLDLCDSWNTTRAAKII
                     SDQLIRSATSVPANIAEGYGGRKGKEFVSYLYQARRSVTETDYWLLLSRERGLSSAEQ
                     TKLLQSGYAGLIRMINTIIGKLNAKEL"
     gene            complement(2684..2757)
                     /locus_tag="AC16GM_08900"
     tRNA            complement(2684..2757)
                     /locus_tag="AC16GM_08900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2721..2723),aa:Pro,seq:tgg)
     gene            complement(2808..3386)
                     /locus_tag="AC16GM_08905"
                     /pseudo
     CDS             complement(2808..3386)
                     /locus_tag="AC16GM_08905"
                     /inference="COORDINATES: protein motif:HMM:NF011397.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
     gene            complement(3415..4266)
                     /gene="murI"
                     /locus_tag="AC16GM_08910"
     CDS             complement(3415..4266)
                     /gene="murI"
                     /locus_tag="AC16GM_08910"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR2923829.1"
                     /translation="MPTTLPAPHPDLVGDSRKQPIGIFDSGIGGLTVAAEIFKKLPHE
                     PVIYFGDTGRYPYGMRSAEVIQKFSRQDANFLLTFGVKLIVVACNTASALALEKIKGE
                     YRVPLVGVIEPGARAAVQATRTGRVGVIGTVGTINSDSYARAIRRIDPAVKVFSLPCP
                     LFVPLAEEGFLDKPATRLIAREYLAPLKKKDIDALVLGCTHYPLLKKVISHVMGKQVR
                     LIDSASETAGAVKAELEVMGLSASDGKQSGRQFFVSDTPEKFVQIGRRFLRKHITRAE
                     KIDITPY"
     gene            complement(4235..5779)
                     /locus_tag="AC16GM_08915"
     CDS             complement(4235..5779)
                     /locus_tag="AC16GM_08915"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR2923830.1"
                     /translation="MKRFRTTPFVLVLGLAALSGASADVKVKQGDRSQTFRSTTIEKE
                     EYVTAQDAAAAVDGKVQWDPVQKKVTFFVGEHKLVFSLFSRYVQVDDRVANLHLLPEF
                     QAGTLWLPLRFLAPIFESIRPEEFIWDQNDKTLRVETGKYNVVDLRASEKLNGTLIEI
                     FTTDPLSFETFLSEDVWLSVNIYKGKLNPAELQKKYLGRYVKEIKAYQFDNAAQVSFR
                     LSKPFLQYRESQAADPDRIQIALEDTSLAKSIDTGAFRPRTTRPGPPKPPVVLPQAKP
                     IQVVVVDAGHGGQDTGATGKKKKTKEKDVVLAIAKKVARKLEADGRFRVVLTRTDDTF
                     IPLEERTKIANDRGADLFVSIHANANRNKSLAGFETYFLDQAVTDEARAVAQMENASL
                     RFEQKDTALARLDDLSFILLDMVQNEHQKESEDLAGLIQGELHRHLPIPDRGVNQAAF
                     FVLNRAYMPAVLVETAFISNAKEEQLLRKGEFQDRIAESIYQAVVQFKTKYEKLNAHD
                     SSRASP"
     gene            complement(5776..6246)
                     /gene="smpB"
                     /locus_tag="AC16GM_08920"
     CDS             complement(5776..6246)
                     /gene="smpB"
                     /locus_tag="AC16GM_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR2923831.1"
                     /translation="MSQTDERKIVATNRKARYEYEILETYEAGLVLAGTEVKSLRGGR
                     ANLQEAYAHPLRGELFVYNLHISPYEAGNRENRDPIRPRKLLLHKKEIRRLAGKIAEK
                     GLTLVPLLLYFKEGYAKLELGLGRGKKLYDRRRAIREREMKREIERAHRERFKA"
     gene            6354..>7128
                     /locus_tag="AC16GM_08925"
     CDS             6354..>7128
                     /locus_tag="AC16GM_08925"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR2923832.1"
                     /translation="MAMFSWIRLRPDSLIFFLLFATSFLLLFSNAEAQQPKAAKRGTL
                     AGTVKSAATGEALPGASVFIKELALGQAADQEGNFRFTNLRPALYTVEVSLIGYVVQR
                     INAVAVRAEEETLQEVLLQTSPLPLGEEIQVIGKRPLLDVNQPQVTRSLDATDLARSA
                     VTELKEAIASQPGAVRFENEIHLRGGRSYENEVIVDGVPASDPLLRSGFGLEINPAVI
                     EQLNLRTGGLPAEYGQATGGVVEILTRDPAPVFSGRVAYR"
BASE COUNT         1397 a         1949 c         2118 g         1664 t
ORIGIN      
        1 ctctttggca ttacggttga acaactgctc accgttggcg gaaagaaaat ggcaccctcc
       61 gaggtctacc gcaagattga gccggtggtg ctaagagccc tagagccgga ggagatggaa
      121 gtggcgaggt tttaaagggc agggatcagt tgaaagcgcg aaaagccagc gtagctctct
      181 gaaatttcct ttacaactca ataaaattcg ttgtatattt acttggcgcg aatgctgacg
      241 ggtggggcgc ctcgcggaaa aggcagactg ttgtggacgg tcgaactaca attcaaaagt
      301 tttttgccag cgagaactgg cggttctgag gagaataaat gtctggtttt gctcgttttc
      361 tttcctccct ctgttctgga tgcctcattt tgttctcttc tttttcgtcc gctcttagcc
      421 aggccagttt gatataccag aaaaatggcg ccgacactac cgctgggttt ggtttctcca
      481 tcgcaagtgc cggggacgtg gacggcgacg gcaaagctga ttttattgtt ggtgctcccc
      541 aatccgggac gccctatacc tccgccggaa caggatcggt ttttgtctat tccggcgcca
      601 ccggcatcct tctgcatcaa aagaacggct ctgtcctttt cgataacttt gggcattcgg
      661 tggcaggtgc cggcgatgtg aatggcgatg gcaaggccga tttcattgtc ggggcgcctt
      721 tcgccgctcc ggctggggtt gaacaggcgg gtgcggtgta tctctactcc ggcggaacgg
      781 gctccctcct cttccagaaa aatggcatcg atcctttcga ttggttcggc cttactgtgg
      841 ccggcgtcgg agatgtcaat ggggatggga gggctgattt cattgtcggg gcaccgctgg
      901 ctgacccggg cggagttgtc aatgccggtt cgatttttgt ctattccggt gccaccggca
      961 cgttgcttta tcggaaagac ggcgccgcct tcctggacaa ctttgggcgt tctgtcgccg
     1021 gggccggaga cattgacggc gacgggagag ccgattttat cgttggcgca cttcttgccg
     1081 accccggagg ggttgaggca gctggttcag cttttgtcta ctccggtgcg actggctctc
     1141 tcctttacca gaaagatggt gccgactccg tcgatttatt tggccgttcc gtagccggcg
     1201 ccggtgatat ggatggtgat ggtagggctg attttatcgt cggtgctccc gatgctaatc
     1261 cgggtggggt tagggacgcc gggacagcct atgtctattc cggagccacc ggcgacctcc
     1321 tcttccagaa aaatggcacc gactccgccg atctgtttgg tttttctgta gccggcgccg
     1381 gggatgtgga tggcgatggc aaagccgatt ttatcattgg cgctcttctt gcggacccgg
     1441 gcggactcac cgacgctggc tcggcctacg ttttttccgg cgccacgggt gctctccttt
     1501 tctctgtgaa cggcacagcg cccctggacg agttgggtca ccaggtggca agtgccggtg
     1561 acgtgcaggg tgatggaaag gccgaggtga ttgtcggagc tcccgaaacc gaccctgagg
     1621 gaattatcga tgccgggtcg gcatttgttt acggcttttg cgtgttgcgc ggggatgtgg
     1681 ataacagtgg agtagccgac atcattgaca tagtgggatt ggtaaggaat gtggttttcg
     1741 gcgatccgtt gcccaatacc gttgctggcg ataccgactg caacgggata tgggacattt
     1801 cggacctggt gctcctggtg cagtacgtgg cgttcgggac gcccacgccg tgctgcctgt
     1861 aaaaagcagg gattaggaat cagaataagg gtgggtagag ggacttgaac cctcaacctc
     1921 ctgggccaca gccaggcgct ctaaccagtt gagctatacc caccgggaaa tacagtgata
     1981 agtgattagt gataaaagtc aagagcaata ttcctacctt tatctgtctg ccggtcgatt
     2041 gtggtttaag attttcgctt ttcatcactc aacactgttt gactgtggtt ttcatcacta
     2101 atcactccat cacccatcac tgtctttcaa tacccccggg ccgattcgaa cggccggcca
     2161 gctgcttaga aggcagctgc tctatccgcc tgagctacgg gggcgaggta agacagtgat
     2221 aaagtgacga gtgatgggtg atgaaaatct atctcacaga tcagagctcc tttgcattca
     2281 gtttgccgat aatcgtgttg atcatccgga tgagtccggc ataaccactc tgcaacagtt
     2341 tcgtttgttc cgcagatgac aacccgcgct cccgtgatag cagtaaccag taatcggttt
     2401 ccgtcaccga ccgccgggcc tggtacaggt agctgacaaa ctctttgcct ttccgtcctc
     2461 catatccttc ggcaatattg gcagggacag aagtggcgga acgaatcagt tgatccgaga
     2521 tgatcttggc agcacgtgtc gtattccagg agtcacagag gtcgagaacg cttaaggtga
     2581 gttcgtgcgc cttctgccag acgacgaggt cacggtaact ctgaaccacc gctttagatt
     2641 gtttttccat cactcgtcac ccatcacttt ttcactgtag taatcggggt gagaggattt
     2701 gaacctccga cgacctgctc ccaaagcagg tgcgctaccg ggctgcgcca caccccgaga
     2761 acgttccctc aatatagaac ggcagaaaag gtcttacaag gaaacgttca ggaggctgga
     2821 gcgtttacca accgggccag gagcgccgcc attttcttga gcgccagtcc gcgatggctg
     2881 atttggtttt tttccgcagt cgaaagctcg gcaaaggttc tcttcagatg cggaatggag
     2941 aaaatcgggt cgtaaccgaa gccgtggctg ccgcgcggct ggtcggttat cactccctca
     3001 attttacctt cagccgtgcg cagatcatcc ggtcccaaag cgagcgccaa gacgcagcgg
     3061 aaccgggcgg tgcgtttttc gagcgggatg ctagcaagca acgagagaag tttgcgatta
     3121 ttgtcggcat acgtggcgtt ttccccggca aagcgggagg aataaacccc gggcgcaccg
     3181 cctaatgcgt ccacctctaa gccggtgtcg tccgccaaag caggaaggcc ggtggcccgg
     3241 tgaatcgatc tggctttcag tttagcgttc tcctctaatg tttttccggt ttcagcgact
     3301 tcggggaaag cagggaaatc ccgataagta agaatttcaa tcggaagttt tttgaggcag
     3361 gaattgattt cttgaatttt gtccgcattc gccggggctc agcccagccg catatcaata
     3421 cggcgtgatg tcgattttct cggctcgcgt aatgtgcttc ctgaggaagc ggcggccaat
     3481 ctgcacgaat ttctcgggcg tatcggaaac gaagaattga cgccctgact gttttccgtc
     3541 acttgcactt aaccccatta cttcaagttc tgctttaacc gctccggcgg tctcggaagc
     3601 cgaatcgatc agccggactt gcttgcccat cacgtgcgag atgaccttct tcaacagcgg
     3661 gtagtgggta cagcccaaaa ccagcgcatc gatatccttt ttcttcaagg gcgctagata
     3721 ctcgcgggcg atcaaacgcg ttgccggttt gtccagaaat ccttcctcgg ctaacgggac
     3781 gaaaagggga caggggagcg aaaaaacttt gaccgcagga tcaatccggc gaatcgcccg
     3841 ggcataagaa tcggagttga tcgtacccac cgtgccgata acgccgaccc gtcccgtacg
     3901 ggtcgcctgc accgcggccc gcgcgccggg ttcaatcacc cccaccaacg gcacccggta
     3961 ctcgccttta attttttcca gcgccagggc cgacgcggtg ttgcaggcga cgacgatcag
     4021 tttgacgccg aaagtcaaaa ggaagttggc gtcctggcgg gaaaatttct gaatgacctc
     4081 agccgaacgc atcccgtacg ggtagcggcc ggtgtcgccg aagtagatga ccggttcgtg
     4141 cgggagtttt ttgaaaatct ccgccgccac ggtcaggccg ccgatgccgg agtcgaaaat
     4201 gccgatcggt tgcttccggc tgtcacccac cagatcaggg tgaggcgcgg gaagagtcgt
     4261 gggcattcaa tttctcgtat ttcgttttga actgcactac cgcctggtag atactttcgg
     4321 caatacggtc ctgaaactca cctttgcgca ggagttgttc ttccttggca ttggagatga
     4381 aggcggtttc caccaggacg gccggcatgt aggcccggtt gagcacgaag aaagcggcct
     4441 gatttacccc ccggtcggga atcggcaggt gccggtgcag ttcgccctgg atcaatcccg
     4501 ccaaatcctc ggactccttc tgatgctcgt tctgcaccat atcgagaagg atgaaactca
     4561 aatcgtccag gcgggccagg gcggtgtctt tctgctcgaa ccggagggag gcgttttcca
     4621 tctgggccac ggcgcgcgcc tcgtcggtca ccgcctgatc gaggaagtag gtttcaaatc
     4681 cggccagcga cttattgcgg ttcgcgttgg cgtggatgct gacgaagaga tcggcgccgc
     4741 ggtcgttggc gatcttggtc cgctcctcca gcggaatgaa ggtgtcgtcc gtgcgcgtca
     4801 aaaccacgcg gaagcggccg tcggcttcca gtttccgcgc cacttttttg gcgatggcca
     4861 gcaccacatc cttctccttg gtctttttct tcttgccggt ggcgccggtg tcctggccgc
     4921 cgtggccggc atccacgacg acgacttgaa tgggcttggc ctgggggagg accaccggag
     4981 gtttgggcgg gcctggccgg gtcgtccgcg gccggaaggc gcccgtgtca atcgatttgg
     5041 cgagcgaggt atcctccaga gcgatctgaa tgcggtccgg gtcggcggcc tgcgactcgc
     5101 ggtactgcag gaacggtttg gagaggcgga acgacacctg ggcggcgttg tcgaactggt
     5161 aagccttgat ctccttcacg tagcggccca ggtatttctt ctgcagttcg gccgggttca
     5221 gtttcccctt gtagatattc accgagagcc agacgtcttc ggagaggaaa gtctcaaaac
     5281 tgagcgggtc ggtggtgaaa atttcaatta acgttccgtt caatttctcc gaggcgcgca
     5341 gatcgacgac gttgtatttg cccgtttcga cccgcagggt tttgtcgttc tggtcccaga
     5401 tgaactcctc cgggcggatt gattcgaata tcggcgccag aaagcggagg ggcagccaga
     5461 gggtgccggc ctggaattcc ggcaggaggt gcaggttcgc cacccggtcg tccacctgca
     5521 cgtagcggga gaaaagtgaa aagacgagtt tgtgctcgcc gacgaagaag gtcaccttct
     5581 tctgcacggg gtcccactgg actttaccgt ccaccgcggc ggcggcatcc tgggcggtga
     5641 cgtactcctc cttttcgatc gtcgtggacc gaaaggtttg cgagcggtcg ccctgcttga
     5701 ctttgacgtc cgctgacgcg ccgctgagag cggccaggcc taaaaccagg acaaaggggg
     5761 tggtgcggaa acgtttcacg ctttgaatcg ctccctgtgg gcgcgttcaa tctcccgttt
     5821 catttcccgt tcgcgaatgg cccggcggcg gtcatacaat ttctttcccc ggcccaggcc
     5881 gagttccaat ttggcatatc cctctttgaa atacaaaagg agcggcacca gcgtcaaccc
     5941 tttttcggca atttttcccg cgaggcggcg aatctctttt ttgtgcagga gcaacttccg
     6001 gggccggatg gggtcacggt tctcgcggtt gcccgcctca tagggagaga tgtgcaggtt
     6061 gtagacgaag agttcacccc gcagcggatg cgcgtaggcc tcctgcagat tcgcccggcc
     6121 gccgcgcaaa gatttcacct cggtgccggc taacaccagg cccgcctcgt aggtttccag
     6181 aatctcgtac tcgtagcgcg ccttgcggtt ggtggcgaca attttgcgtt catctgtttg
     6241 gctcattgac ttccgctcat ttccaatata tcggctaatt tccgccgcgc gcaatctcct
     6301 tgacaggtca aggcgaaaaa gtcattttac agcgaagtgc cggttgagct atgatggcca
     6361 tgttctcctg gattcgtttg agaccggact cgctcatctt ttttctccta ttcgccacct
     6421 ctttcctcct tttgttctcc aacgcagagg cgcaacagcc caaagccgcc aaaaggggta
     6481 cgttggccgg aactgtaaaa agcgccgcga ccggagaggc tttgcccggt gccagcgttt
     6541 ttattaagga actagctttg ggtcaggccg ccgaccaaga ggggaacttc cgcttcacga
     6601 atttgcgtcc ggcactctac accgtggagg tctcgcttat cggctacgta gtccagcgca
     6661 tcaacgcggt ggcggtccgg gcagaagagg aaacgttgca ggaagtgctg ttgcagacca
     6721 gtcccttgcc tctgggtgaa gaaattcagg tgatcggcaa gcggccgctt ttggacgtca
     6781 accagccgca ggtgacgcgc tcgctggacg ccaccgacct ggcgcgctcg gcggtcaccg
     6841 aactgaaaga ggcgatcgcg tcccagccgg gagcggtgcg ctttgagaac gaaatccacc
     6901 tgcggggagg gcgcagttac gaaaacgaag tgatcgtgga cggcgtgccg gcctcggacc
     6961 cgctcctgcg ctccggattc gggctggaaa tcaatccggc ggtgattgag cagttgaacc
     7021 tgcgcacagg gggcctgccg gcagagtacg gccaggccac aggcggcgtg gtggagattc
     7081 tgacgcgtga tcccgcaccc gtcttctcgg gtcgggtagc gtaccgga
//
LOCUS       DBNGZD010000333         5481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1720663, whole genome shotgun sequence.
ACCESSION   DBNGZD010000333 DBNGZD010000000
VERSION     DBNGZD010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1720663"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="AC16GM_08930"
     CDS             <1..730
                     /locus_tag="AC16GM_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR2923833.1"
                     /translation="VAYPLTRYDICVMSDGAAVAFLASEEGIKRIEKKTKRKIKPALV
                     TGVGRGTDAMRFADRPREKVILLPHEKASDYKDLEYPGVHSFRAGRMAGKLAYEMAGI
                     GDPLNELDFIELHDAYSSSELQTYEDLGLCKYGEGGKFVDAGYPFLPNLEYNFGEEQS
                     SKQFKAKKRIPVNPSGGLIACGHPVGATGLMQAVFALWQLQGTVKKHFKNDSLQVPNA
                     RRGIIHSHAGTGTYVTVSVMERVD"
     gene            744..1988
                     /locus_tag="AC16GM_08935"
     CDS             744..1988
                     /locus_tag="AC16GM_08935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2923834.1"
                     /translation="MAEVQTLPKESVAEKTEPAKPRSRFDEIWGGGRPIWNWDPRQEA
                     VRRLGREFCEREVVPVAKKLDQVVPQQFAFDLYYKVAKAGFVGFSMPVEYGGGGRNSL
                     EYNTLVETLAYYDLSTCLLAAVGELATHPIIHFASEEQKRKYVPKCASGELISAYLLT
                     EPEAGSDAANQRTVAVPDGDHYVVNGEKIFIMHGDVAHLGVLFCRIQEEGARDKVSAL
                     IVDLTSPGVKRTTLQQKMGIRAATTGRVWFENVRVPKENLLGEKGRGFRYALATLDSA
                     RIGVAAQGVGMAQRAFDEAVGYAKKRIAFGNPIAKLQAIQWMIADMATRIEAARVLTY
                     KAALLQEQGVKFSLEGSEAKVCATETARFCVDRCMQIHGGYGYIGEFTEVEKLYRDQR
                     ILEIYEGTSEVQRLVIAKHYCG"
     gene            2046..2888
                     /locus_tag="AC16GM_08940"
     CDS             2046..2888
                     /locus_tag="AC16GM_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2923835.1"
                     /translation="MKLGRFEISHFVENRFHLDGGTMFGVVPKTMWSKLIPADERNLI
                     PMDENIFLVKAHGKNVLLDVGLGDVLSDRDRKVYNCYTPSRLEDGLKAIGLAPDDVDF
                     VLLTHLHTDHCGGAWKLDEKGKVVPRFRKARHIIQKSEWEDALHPDERTSAVYLADRL
                     RVLGETNLVDLVDGEGQVIPGITITRTGGHTRGHQAVLVESDGHRLVAYADVIPTTSH
                     LRSAYVASVDIFPLDTMKVKKQVIPRLVQDGTLVAFDHDLKIKIGRIVERGDKLVAES
                     VTLA"
     gene            2895..4448
                     /locus_tag="AC16GM_08945"
     CDS             2895..4448
                     /locus_tag="AC16GM_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR2923836.1"
                     /translation="MTTPIEEKIAYLKKLRAEAKLGGGEKRIEEQHRKGKLTARERLE
                     ILLDENSFEEFDAFVTHQTTAFGLDKQKYLGDGVVTGYGTIYGRPVFVFSQDFTVFGG
                     SLSGAYAEKICKIMDLAMKVGAPVIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGV
                     LPQISVVLGPCAGGAVYSPAITDFIFMNRTAYMFVTGPSVVKTVTHEEVDSEALGGAQ
                     VHATKSGVAHVVGENEADTLVKVRQLMRFLPQNNVDDPPVLPSDDPADRMEESLNTLV
                     PENPNKPYDIKDVIRRVADHGEFFEIQEQYAQNIVVGFARFGGKPVGIVGNQPAVLAG
                     VLDIDSSLKGARFVRFCDAFNIPLVTFVDVPGFLPGTVQEWGGIIKHGAKLLYAFAEA
                     TVPKITVITRKAYGGAYDVMSSKHIRADLNYAWPTAEIAVMGPKGAVEILFKKEIAES
                     KDPEKALEEKVAEFREKFANPYTAAERGYVDDVIEPKETRPRIIRALQMLETKKDTNP
                     PKKHGNIPL"
     gene            4477..4896
                     /locus_tag="AC16GM_08950"
     CDS             4477..4896
                     /locus_tag="AC16GM_08950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2923837.1"
                     /translation="MKIRNATTTDFPFIQRSIKEWLLDYENLTPEQFIVVEEDDRILG
                     FGRLKPYPDGTVELGCVGVIPERRKQGIGEMITNELIRRGPDTIYITTDLSNYFVPFG
                     FKLTSEYPEAIKKKLAWCETGTCRPGVVAMVLKKGNK"
     gene            4893..>5481
                     /locus_tag="AC16GM_08955"
     CDS             4893..>5481
                     /locus_tag="AC16GM_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923838.1"
                     /translation="MKALGKCFVIFMFIVVCPGLAGAYLNPSLRIRALGTDFIGLVPD
                     ERTDIFRNPAYLAMLSRGNVSGEFANQVDFWLSGVLPLSRYGVLAYSQTWKVNETSRP
                     NDIFPGRQYDVNLRGQYGLRVAERIHLGVDYTYLWNKTRSSESNPDAVRYRPISQTAR
                     FGFSWKVDKGFSVTALVKGEWAKQREKILWFHSPTD"
BASE COUNT         1244 a         1526 c         1631 g         1080 t
ORIGIN      
        1 ggtggcctac ccgctgaccc gctacgacat ctgcgtgatg tccgacggag ccgcggtcgc
       61 cttcttggcc agtgaggagg ggatcaagcg catcgagaag aagaccaaaa gaaaaatcaa
      121 gccggcgctg gttaccggcg tgggccgggg gaccgatgcc atgcgttttg ccgaccgtcc
      181 ccgcgaaaag gtgatcctcc tgccccacga gaaggcgtcg gactacaaag atctcgaata
      241 tccgggcgtt cattccttcc gcgccggaag gatggccggg aaactggcct acgaaatggc
      301 gggaatcggc gacccgctca acgaactcga cttcatcgaa ctgcacgacg cctactcctc
      361 atccgaactc cagacctacg aggatttggg gctctgcaag tacggcgaag gcgggaagtt
      421 cgttgatgcc ggctacccct tcctgccgaa cctggagtac aacttcggcg aggagcagtc
      481 gagcaagcaa ttcaaagcca aaaagcgcat tccggtgaac ccctccgggg ggttgatcgc
      541 ctgcggccac ccggtgggcg ccaccggcct gatgcaggcg gtctttgccc tctggcagct
      601 gcaggggacg gtgaagaaac actttaagaa cgactcgctg caggtgccga acgcacggcg
      661 gggaatcatc cacagccacg caggcacagg cacctatgtt accgtttcgg tcatggaaag
      721 agttgactga gaaaggactg gctatggcag aagttcagac tttgccgaaa gaatctgtgg
      781 cggagaagac ggaaccggcc aagccgcgct cgcgtttcga tgagatctgg ggcggcggcc
      841 ggcccatctg gaactgggat ccccgtcagg aagcggtgcg gcgactgggc cgcgagttct
      901 gcgaacgcga agtggtgccg gtggccaaaa aacttgacca ggtggtaccg cagcaattcg
      961 ccttcgacct gtactacaaa gtcgccaaag ccggtttcgt cgggttcagc atgccggttg
     1021 aatacggcgg cggcggcaga aactcccttg aatacaacac gctggtggag acgctggcgt
     1081 actacgatct ttccacctgc cttctggccg cagtgggcga attggccacc caccccatca
     1141 ttcactttgc cagcgaggag caaaagcgga aatacgtgcc gaaatgtgcc tcgggcgaac
     1201 tgatcagcgc ctatcttttg accgaacccg aagccggctc cgatgcggcc aatcaaagga
     1261 ctgtcgctgt ccccgacggc gaccactatg tcgtcaacgg cgagaagatt ttcatcatgc
     1321 atggcgacgt ggcgcactta ggtgttctct tctgccggat tcaagaagaa ggggcgcggg
     1381 acaaagtctc ggccttgatc gttgatctga cgagcccggg cgtcaagcgc accacgttgc
     1441 agcagaaaat gggcatccgc gccgccacca ccggccgggt ctggttcgaa aacgtgcggg
     1501 tgccgaagga aaatctttta ggcgaaaaag ggcgcgggtt ccgctacgcc ctggcaacct
     1561 tagacagcgc ccgcatcggc gtggcggcgc agggcgtcgg catggcccag cgggcgtttg
     1621 acgaagcggt gggctacgcc aagaagcgga tcgccttcgg gaatccgatt gccaagctgc
     1681 aggcgattca gtggatgatt gccgatatgg ccacgcggat tgaagccgcg cgcgttttga
     1741 cctacaaggc ggcgctcctg caggagcagg gagtcaaatt cagtctcgag ggctcggagg
     1801 cgaaagtctg cgccactgaa acggcgcgct tctgcgtgga tcgctgcatg caaatccacg
     1861 gcgggtacgg ctacatcggc gagttcaccg aagttgaaaa gctctaccgc gaccagcgga
     1921 tcttggaaat ttacgaaggc acctccgagg tgcagcggct cgtgatcgcc aagcattact
     1981 gtggatagag ccagagttcc taccgtcgcc ttccctcgag ccgatggcac tgcctgcccc
     2041 cctcgatgaa actaggccgt tttgagattt cccacttcgt cgagaaccgc ttccacttgg
     2101 acggcggcac gatgttcggc gtggtgccca agacgatgtg gtcgaaactc attcccgccg
     2161 acgaacggaa tcttatcccg atggatgaga atattttcct ggtcaaggcg cacgggaaga
     2221 acgttctctt agacgtcggg ctgggggatg tcctttctga ccgcgatcgg aaagtttaca
     2281 actgctacac gccgtcgcga cttgaagacg gattgaaagc gatcggactg gcgcccgatg
     2341 acgttgattt cgtccttctc acgcacctgc acaccgacca ctgcggcggc gcctggaaat
     2401 tagacgaaaa aggaaaagtc gtcccgcgat tccgcaaggc ccggcacatc atccagaaat
     2461 ccgagtggga ggacgccctc caccccgacg aacgcacgtc cgccgtctac ttagccgatc
     2521 ggttgcgcgt gttgggggaa accaatttag ttgacttagt cgatggcgaa gggcaggtca
     2581 taccgggcat cactatcacc cggaccggcg ggcatacgcg cgggcaccag gcggttctgg
     2641 tggaaagcga cggccaccgg ttggtggcct acgccgacgt catccccacc acgtctcatc
     2701 tccggtcggc gtacgtcgcc tcggtcgata tcttcccgct ggatacgatg aaagtcaaaa
     2761 agcaggtaat cccgcggctg gtacaggacg gaaccttagt agcttttgac cacgacttga
     2821 agatcaaaat cggacggatc gttgaacgcg gcgacaaact ggttgccgaa tcagtcactc
     2881 ttgcctgact tcctatgacc acgcctattg aagaaaaaat cgcgtatctc aaaaaactcc
     2941 gcgccgaagc gaaattgggc gggggggaga agcggattga ggagcagcac cggaagggga
     3001 aactgaccgc ccgggaacgg ctcgaaattc ttttggacga aaattcgttc gaagagttcg
     3061 acgccttcgt cacccaccag actaccgcgt tcggactcga taagcagaag tatctgggcg
     3121 acggcgtggt gaccggctac ggcaccatct acggccgtcc ggttttcgtt ttctcccagg
     3181 atttcaccgt tttcggcggt tcgctctccg gcgcctacgc cgagaaaatc tgcaagatta
     3241 tggatctggc catgaaggtg ggcgcgccgg tgatcggatt gaacgactcc ggcggggcgc
     3301 gaattcagga aggggtggtg agtctgggcg gctacgccga catcttcctg cgcaatacgc
     3361 ttgcctccgg cgtcctcccg caaatttcgg tcgttctcgg tccctgcgcc gggggcgcgg
     3421 tctactcgcc ggccatcacc gacttcattt tcatgaatcg aaccgcctac atgttcgtca
     3481 ccgggccctc ggtggtcaaa acggtgacgc acgaagaggt cgattctgaa gcgttgggcg
     3541 gggcgcaggt gcatgccacc aagtcgggcg tggcgcacgt ggtcggcgaa aatgaagccg
     3601 atacgctggt gaaagtccgg caactgatgc gctttttgcc ccagaataac gtcgatgacc
     3661 cgccggttct gccatccgat gatccggcgg accggatgga agagagtctc aacacgctcg
     3721 tgcccgagaa cccgaacaaa ccgtacgaca tcaaggacgt catccgccgg gtggccgatc
     3781 acggcgaatt tttcgaaatc caggaacagt atgcgcaaaa catagtcgtt ggttttgcgc
     3841 gcttcggcgg aaagccggtg gggattgtcg gcaaccaacc tgcggtctta gccggcgtgc
     3901 tggacatcga ctcttcgctc aaaggcgccc gcttcgtgcg gttttgcgac gcgttcaaca
     3961 ttccgctcgt caccttcgtg gacgttcccg ggtttctgcc cggcacggtc caggagtggg
     4021 gcgggatcat caagcacggg gcgaagctgc tctacgcttt cgccgaggcg acggtgccca
     4081 aaatcacggt catcacccgc aaggcctacg gcggggcgta cgacgtcatg tcgtccaagc
     4141 acatccgggc cgacctcaac tacgcctggc cgacggcgga aatcgccgtc atggggccca
     4201 aaggggccgt ggagattctc tttaaaaaag aaatcgccga atcaaaagac ccggagaagg
     4261 cgctggaaga gaaagtcgct gaattccgcg aaaaatttgc caacccctac acggcggcgg
     4321 agcgggggta cgttgacgac gtgatcgagc cgaaagaaac gcgcccgcgc atcatccgcg
     4381 cattgcaaat gttagaaacc aaaaaggaca ccaacccgcc gaagaagcac gggaatatac
     4441 cgttgtaaga gatctccttc acccaggtcg tataagatga aaatccgcaa cgccacgacc
     4501 acagatttcc cattcattca acgctctatc aaggagtggc tgcttgacta tgagaatctt
     4561 acgcctgaac agtttattgt agtagaagag gatgaccgga ttctgggctt cgggcggctg
     4621 aagccgtatc ccgacggcac ggtggagtta gggtgcgtgg gagtgatccc ggaaaggcgc
     4681 aagcagggta tcggggaaat gatcaccaac gaactgattc gccgggggcc ggacacgatc
     4741 tacatcacca ccgacctgtc aaactacttt gttccattcg gtttcaaact gacctcagaa
     4801 tacccggaag cgatcaaaaa gaaactggcc tggtgcgaaa ccggcacctg ccgccccggt
     4861 gtggtggcga tggtgctgaa gaaaggaaac aagtgaaagc actgggaaag tgtttcgtaa
     4921 tctttatgtt tattgtggtt tgcccaggcc ttgccggtgc gtatttgaat ccttcactcc
     4981 gcatacgtgc tctggggaca gactttatcg gactggtccc ggacgaaagg acggacattt
     5041 ttcgaaatcc ggcttacctt gctatgctta gcaggggtaa cgtcagtggt gaatttgcca
     5101 atcaggttga tttttggttg agcggggttt tgcccttatc tcggtatgga gttctcgcct
     5161 actctcaaac atggaaagtc aatgagactt ccagacctaa tgatattttt ccaggtcggc
     5221 agtatgatgt taatcttagg gggcagtatg gattacgggt ggcagagaga atccatctcg
     5281 gtgttgatta cacttatctc tggaacaaaa caagatcatc cgaaagcaac ccagatgctg
     5341 ttcggtatcg accaattagt caaactgcac gcttcggctt ctcttggaag gtagataaag
     5401 ggttctcggt tactgctctg gtgaaaggag aatgggcgaa gcagagagaa aaaatccttt
     5461 ggttccactc gcctactgac a
//
LOCUS       DBNGZD010000334         5244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_192526, whole genome shotgun sequence.
ACCESSION   DBNGZD010000334 DBNGZD010000000
VERSION     DBNGZD010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_192526"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="AC16GM_08960"
     CDS             complement(<1..665)
                     /locus_tag="AC16GM_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR2923839.1"
                     /translation="MPTAAIIDNVLAAIGDTPLVRLKKVTPPGGAEILAKAEFLNPGG
                     SVKDRMALYIIEQAERSGLLTPGGTIVENTSGNTGVGVAMVAALKGYRAIFTMPDKMS
                     TEKINFLKAFGAKVIVTPTNVPADSPLSYYETAKRIARETPNSFYLNQYHNPLNIEAH
                     YKITGPEVWEQTQGKLDCCVAGIGTGGTLSGVGRFLKEKNKNIKVIAVDPVGSVFHHY
                     FKTG"
     gene            793..2979
                     /locus_tag="AC16GM_08965"
     CDS             793..2979
                     /locus_tag="AC16GM_08965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR2923840.1"
                     /translation="MQKLLDDLNPPQREAVTHPSGPLLILAGAGSGKTRVLTYRIAHL
                     IAERGVPAEQVLAVTFTNKAADEMRARIRTILGVEKIPLVMSTFHSFCARLLRREAEL
                     LGFPRTFSIYDDDDQVGLLKHIIEDLQLQNRQLSAAGAQNQISLAKNRLQSPADFAAD
                     ARPGSGGTKGLVAQIFAEYQKRLQQQGAMDFDDLLVKVVELFRRHPERLAYYQNHFAQ
                     ILVDEYQDTNHAQYLLVKFLAEKHRNLAVVGDDDQSIYRWRGADLRNILEFERDFPDA
                     RVVKLEQNYRSPQVILDAAWQVVSRNTGRKEKKLWTDREGGEKISLTLTFDDRMESRW
                     VAEEIARRRAALDLSFSDFVVLFRTNAQSRLLEDALRDRGFPYVIVGGVRFYQRREVK
                     DVIAYLRVLANPRDDTACLRILNVPRRGIGAATEKKLIDLAREKNLPLFELLATPELW
                     PRSSPKIKKELQKLSAFLTDLKPRVAALPVAELIETIVMRTGLVEELQVEDAQAQESR
                     AENVKELIAAAAEFADRTENPTVDTFLEEVTLLSDIDRWDDAQERITLMTLHQAKGLE
                     FRVVFLAGMEEGLFPLSRSLADPDELEEERRLFYVGCTRAKEKLYLSACRHRRRFGEM
                     TNLKSRFLDELPPEHLETTDLGFSTTISWEAAQRSARQVELIPEFDAVTARVKVGSRV
                     IHPTWGQGKVLQREGSGENLRLLVQFTSGGPKKLLAKYANLEIAQY"
     gene            complement(3053..4096)
                     /locus_tag="AC16GM_08970"
     CDS             complement(3053..4096)
                     /locus_tag="AC16GM_08970"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR2923841.1"
                     /translation="MVLWGTLLVFPLALSGCSSKAELSQIENSVASVMDKVGPDVVFI
                     SATLPPAKEKLQPTVRRGCGVILKDGRILTTSSLLAGAKECTIRFQDGSQVAPEGIAR
                     ICADFETNVGVIVLKGASRKNADFAVAKNVKPGVIGIALGNTPYSKGLAVAVGAIGVS
                     WVGGVDAYDTNLLTFDAPHLGSYSGIPIFNSKGELVGLIEGRFADTESPWLVVPATTA
                     LAIAERLEKDSCIERGFLGVFSHTNCQEEYGGYDRVVDPKKPAGVYLSGVVPGSPAAK
                     AGFREGDLIFSINGRPVNSCVALRKLVTAVKAGDDVVVEYRHQGKVFKRTIAVDNLPP
                     CCLSSRRCSDRPT"
     gene            complement(4138..4605)
                     /locus_tag="AC16GM_08975"
     CDS             complement(4138..4605)
                     /locus_tag="AC16GM_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR2923842.1"
                     /translation="MIDCRICNKLHNRYDFQSDVWAKEMEAHCRACPPCQQEFLLYQS
                     VDAELKQAFREEPPARLWEGFWAELARKLRGRSWREKVGDQLESVWLWLNRPVVGFVP
                     AYALAALLVLALSVGLITRSGRPGFQSDLVVHPQQSVSAEVVDGVTVYQIASR"
     gene            complement(4609..>5244)
                     /locus_tag="AC16GM_08980"
     CDS             complement(4609..>5244)
                     /locus_tag="AC16GM_08980"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2923843.1"
                     /translation="DPAERLKEEPETTLHGAPGGADGFKEDPAVLALIERVKEKDDRA
                     FQELVERYRNQVAGLAYKMVGDYDDAKDISQIVFVKTYYNLQRFDTTKKFSTWLYRIT
                     INASIDYWRKYHKHRHEPLEEVINRPDESQAGPEVQYQRKKTREEISRALVALNDKQR
                     QVFVLRDMEGLDIAEVAKVMNMPQVTVRWYLHRARTKLRTQLVKKRRRMGG"
BASE COUNT         1092 a         1561 c         1508 g         1083 t
ORIGIN      
        1 cccgtcttga agtagtgatg gaaaaccgaa ccgaccgggt ccaccgcgat aaccttgatg
       61 tttttgttct tttccttgag gaagcgcccc accccggaga gcgtcccgcc ggtgccgatc
      121 ccggccacgc agcagtcgag tttcccctgg gtttgctccc agacttccgg tccggttatt
      181 ttgtagtgcg cttcgatatt caggggattg tggtattgat tgagatagaa cgaattgggc
      241 gtttcgcggg caatccgctt ggccgtttcg tagtacgaaa gaggcgaatc ggccgggaca
      301 ttggtggggg tgacgatcac tttggcgccg aaggccttga ggaaatttat cttttcggtc
      361 gacatcttgt ccggcatggt gaagatagcc cggtaccctt tgagggccgc caccatcgcc
      421 acgccgacac cggtgttgcc ggaggtattt tccacaatcg tccctccggg cgttaaaaga
      481 ccgctccgct cggcctgttc gatgatgtag agggccatcc ggtctttgac cgaaccgccg
      541 ggattgagga actcggcttt agccagaatt tcggcgcccc ccgggggggt gactttcttc
      601 agccgcacca gtggagtatc gccgatagcg gctaagacat tgtcgataat ggctgcggtc
      661 ggcatcgcct gcacactctc ggagccgtaa caaaaacctt tttggccttg ctgtcaatga
      721 cgcggttgcg taaaatgcag tggccatcag gccaagggcg atagtgttca aaggaaaaag
      781 taaggagaaa agttgcaaaa actattggat gatttgaacc cgcctcagcg ggaggcggtc
      841 acccatccgt ccggaccgct tttgattctg gccggcgccg gttcgggtaa gacccgcgtc
      901 ctgacctacc ggattgcaca cttgatcgcc gagcgcgggg tgccggcgga acaggttctg
      961 gcggtgacct tcaccaacaa agccgccgac gaaatgcgcg cgcgcattcg caccatcttg
     1021 ggagtggaga aaattccttt ggtgatgagc acgttccatt ctttctgcgc ccggctcctg
     1081 cgccgcgaag cggaactttt gggcttcccg cgcacttttt ccatctacga tgatgatgat
     1141 caagtgggct tactcaagca tattatcgaa gatctccagc tgcaaaaccg gcaattgtcc
     1201 gccgcgggag cgcagaacca gatcagcctg gcgaaaaacc ggctccagag tcccgccgac
     1261 ttcgccgccg atgcgcgccc gggttcggga ggcaccaagg ggctggtagc ccagatattt
     1321 gcggaatacc agaaacgttt acaacaacag ggagccatgg atttcgatga cctgctcgtc
     1381 aaggttgttg aactgtttcg gcgccacccc gagcgcctcg cttattacca gaaccacttt
     1441 gcccaaatcc tggtggacga gtatcaggat accaaccacg cccagtacct cttggtgaaa
     1501 tttctggccg aaaagcaccg caatctggcg gtggtggggg acgacgacca gtcgatctac
     1561 cgctggcggg gcgccgacct gcggaacatc ctggagttcg agcgcgactt ccccgacgcc
     1621 cgggtggtga aattggagca aaactaccgc tccccgcagg tgatcctgga tgccgcctgg
     1681 caggtggtgt cgcgcaacac gggaaggaaa gaaaagaaat tgtggactga ccgcgaaggc
     1741 ggcgagaaaa tcagcctgac gttgaccttt gacgatcgga tggaatcccg ctgggtggcg
     1801 gaagaaattg cccggaggcg ggcagcgcta gacctgtcat tttccgattt tgttgtgctc
     1861 ttccggacca acgcccagtc gcgcctgttg gaagatgcgc tgcgggatcg gggttttccc
     1921 tacgtcattg tcggaggagt gcgcttttac cagaggcgcg aggtaaagga cgtgattgcc
     1981 tacctgcggg tgctcgccaa tccccgggac gataccgcct gcctgcggat tctgaacgtg
     2041 ccccggcgcg gcatcggagc ggcgaccgaa aagaagttaa tcgatttggc ccgcgaaaag
     2101 aacctgcccc tgttcgaact tctcgctacg cctgaactgt ggccccgctc ctcacccaag
     2161 atcaaaaaag aactgcagaa actctcggcg tttctcaccg acctgaagcc cagggtggca
     2221 gcgctcccgg tggccgagct tatcgagacc atcgtgatgc gcaccggctt ggtggaagag
     2281 ttgcaggtcg aagatgcgca ggcccaggaa agccgggcgg aaaacgtgaa ggaactgatt
     2341 gccgccgccg ccgaatttgc cgaccgaacg gaaaatccga cggtagatac tttcttagaa
     2401 gaggtgacgt tattatcgga catcgaccgc tgggacgacg cccaggaacg cataaccctc
     2461 atgacgttgc atcaggccaa agggctggag ttccgtgtgg tattcctggc cggaatggag
     2521 gagggacttt tcccgctctc ccgatcgctg gctgatccgg atgaactgga agaggaacgg
     2581 cggttatttt acgtcggctg cacccgggcg aaagagaaac tctacctctc cgcctgccgc
     2641 caccgccgcc gtttcggcga aatgaccaac ctcaaatcgc gctttctgga tgaactaccc
     2701 ccggagcatt tggaaacgac cgacctcggt ttctccacca ccatctcctg ggaagcggct
     2761 caacgctcgg cgcggcaggt ggaactgatc ccggaattcg atgcggtcac cgcccgtgtc
     2821 aaggtcggtt cccgggtaat tcaccccacc tggggacagg gcaaagtact ccagcgggaa
     2881 ggttcaggtg agaatcttcg actgttggtg cagttcactt ccggcgggcc gaagaaactt
     2941 ctggccaaat acgccaattt agaaatcgcc caatactgag ggactcgcag aatatttagg
     3001 aatcagtttt tagaaacggg aaatagtcgc cctattctcg gatcgctctt ctttaggtgg
     3061 gtcgatccga acagcggcgt gacgaaagac agcacggggg gagattatcc accgcaatcg
     3121 tccgtttgaa gactttcccc tggtgccggt attcaacgac gacgtcgtca cctgctttca
     3181 ccgcggtcac cagtttgcgc agggccacgc aggaattcac cggccggccg ttgatggaga
     3241 agatgaggtc cccctcccga aagccggcct tggcggcagg gcttcccggc acgacgccgg
     3301 agaggtacac tcccgccggt ttcttgggat caaccacccg gtcgtatccc ccgtattctt
     3361 cctggcagtt ggtgtgcgag aaaacgccca gaaacccgcg ctcaatgcag gaatctttct
     3421 cgagccgctc tgcgatggct aaggccgtgg tcgccggaac gaccagccag gggctttcgg
     3481 tatcggcgaa ccggccttca atcaaaccga ctaactcccc cttgctgttg aaaatcggaa
     3541 tgccggagta actgccgagg tgcggagcat caaaagtcaa aaggttcgtg tcgtaggcat
     3601 ccacgccgcc cacccacgaa acgccgatcg cccccaccgc cacggccaaa cccttggaat
     3661 aaggggtatt ccccagggcg attccgatga caccgggttt gacgttcttc gccacggcaa
     3721 aatcggcatt ttttcgggac gcacctttga ggacgatcac gccgacattg gtttcaaaat
     3781 cggcacagat tcgcgctata ccttccggag caacttggct tccatcctgg aagcggatgg
     3841 tacactcctt ggctccggcc aggaggctgg aggtagtgag aatccggccg tctttgagga
     3901 tcacgccgca accccggcgc acggtcggct ggagtttttc tttagccggc ggcagggtgg
     3961 cggagatgaa cacaacgtcg gggccaacct tgtccatcac cgacgcaacg gaattttcta
     4021 tctgggaaag ctcggctttg ctggaacagc cggagagcgc cagcggaaag acaagaagcg
     4081 taccccacag aaccaccctc cccctccggc gccgcagaac gggaagattc atacgtctta
     4141 acgggaagcg atctggtaaa cggtaacgcc gtccaccact tcggcggaaa ccgattgctg
     4201 ggggtggaca acgaggtcac tctggaaccc gggtcggccg ctgcgggtaa tcaaacccac
     4261 ggagagagcc aggaccagca gggcagccag ggcataggcc gggacaaacc ccaccaccgg
     4321 ccggttcaac cagagccaca cgctctccaa ctggtcaccc actttctctc gccagctgcg
     4381 gccccggagt ttgcgcgcta attcggccca aaacccttcc cacaatctgg ctggcggctc
     4441 ttcgcggaac gcctgcttga gttcggcatc caccgactgg tagaggagaa attcctgctg
     4501 gcagggcgga cacgcccggc agtgggcttc catctctttc gcccacacat cgctctggaa
     4561 atcgtaccgg ttgtggagct tgttacaaat acgacaatcg atcacgcttc atcctcccat
     4621 ccgcctccgc tttttgacta actgtgtccg caacttggtc cgcgcccggt gcaggtacca
     4681 ccgcaccgtc acctgcggca tgttcatgac cttggcgact tcggcgatat ccaatccttc
     4741 catatcccgc agcacaaaga cttggcgctg cttatcgttc aaagccacca gcgcccgact
     4801 gatctcctcc cgcgttttct tccgctggta ctgcacctcc ggccccgcct gtgattcgtc
     4861 gggccggttg atcacttcct ccagcggttc gtggcggtgc ttgtggtact tgcgccagta
     4921 gtcgatggag gcgttgatcg taatccggta cagccaggtg gaaaatttct tggtggtatc
     4981 gaaccgctgc aggttgtagt aggtcttgac gaacacgatc tgggagatgt ccttggcgtc
     5041 gtcgtaatct cccaccatct tgtacgccag acccgccacc tgattgcggt agcgttccac
     5101 cagttcctgg aaggcccggt cgtccttctc cttgacccgt tcgattaagg ccagaaccgc
     5161 cgggtcttct ttgaacccat cggctccacc tggtgcacca tggagagttg tctccggctc
     5221 ctctttcagg cgctcggccg ggtc
//
LOCUS       DBNGZD010000335         4093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1860321, whole genome shotgun sequence.
ACCESSION   DBNGZD010000335 DBNGZD010000000
VERSION     DBNGZD010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1860321"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="AC16GM_08985"
     CDS             complement(<1..129)
                     /locus_tag="AC16GM_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR2923844.1"
                     /translation="MQEFLQQVFNGIAWGSIYALIALGYTMVYGVLKLINFAHGDVF"
     gene            complement(133..1278)
                     /locus_tag="AC16GM_08990"
     CDS             complement(133..1278)
                     /locus_tag="AC16GM_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2923845.1"
                     /translation="MRKSFGWVLASLFILGTFSTCAKQGNVIKVGEYGSLTGTTATFG
                     ISTKNGIDMATEELNAGGGLLGKKVQIIVEDDQGKPEEAATAVQKLISQDKVVAVLGE
                     VASTRSMAGGRICQQNKIPMVSPSSTNPAVTQIGDYIFRVCFIDPFQGEVMAKFCRNT
                     LKMKRVAILKDVKNDYSVGLSEYFAKTFKESGGQVVAEEAYTEGDQDFKAQLTTIKTK
                     RPETIFVPGYYTEVGLIARQARELGITVPITGGDGWESPDLLKVGGAALDNCYFSTHY
                     SSDDPRSEVQNFVATYKQKYGQAPDAIAPLAYDAARILFAAIQKANSTDGDAVRRVLA
                     ETKDFSGVSGQITIDANRNAVKPAVVLKIENGQYKFVEQIAPGTPAP"
     gene            1459..2202
                     /locus_tag="AC16GM_08995"
     CDS             1459..2202
                     /locus_tag="AC16GM_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR2923846.1"
                     /translation="MSTQILLVLLTSVLAGGANVIGGLIVASRAGWTKQLLRYCLAFG
                     AGFILAAAALRMIPISIQARPDAALLVLAGYFFVHLFEHTVSPHFHFGEETHAVEAGL
                     TRQVGFSAVFGLVMHAFFDGVTISSGFAVTPALGLLLFLAILLHKLPEGFSVASLALA
                     SGHSRRWAVGAAAVVGISTVVGGVAASFLSSLMTYALAFSAGVAIYVAATDLIPEVNR
                     EPGIKMSFMVFLGVALFYITERLLEMLGV"
     gene            complement(2298..>4093)
                     /locus_tag="AC16GM_09000"
     CDS             complement(2298..>4093)
                     /locus_tag="AC16GM_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2923847.1"
                     /translation="DLYDIDYSTNGIGDWSVMVGGSYNGGSRVPAQFDAWCKVTLGWV
                     TPTNYVANADSQTLARVEDNPQVFRLWRGGAIGPQYFLVENRQRKLFDRFLPGDGLLI
                     YHIDETVPGNWDENHRLVDIEQADGLRQLNNSGSQGDAADPFPGATNNRTFDDLSNPN
                     SRDYGNQHTQVGVWNVSNSDSLMTANIEVFFSRPALAIASFWLDDTTFGNGDGVIDSA
                     ETVVLRLTVTSLMAAASGVSVVAQADNADILFSDRTSSLGTIASLDSVSNSADPIVFS
                     LAPTLPLPRTVYFSLEVATADSLSVIQATVKGTLGRPRVLIVDDDSEKSYESYYTGAL
                     DSLREPYGFRTASLVSTADFASYPTAFWFTGDQRLPALAHQSVLKLQTFLDNGGQLFL
                     SGQSLAEGLAAGPDSTFLTNYLHTRYAGNNPITSSLYTVGKPGDPITDSKKVWIMGAD
                     GAVNLPSPDYFSPLPQGPAILTDMSTGRAAGVRYGANYRVVFLGFGFEGITNTRTAQG
                     YETREQFLSDVLNWLVTGRPTYLLGDVSDDGVVDLLDLVWLLQYELSGNPAPKPILQV
                     GDVNCDTAYDMQDLVTLLNHVVFGDPLPCAP"
BASE COUNT          779 a         1151 c         1254 g          909 t
ORIGIN      
        1 aaaaacgtcg ccgtgggcaa agttgatcaa cttgagcaca ccgtagacca tggtgtagcc
       61 cagagcgatc aaggcgtaga tggagcccca ggcaatgccg ttgaagacct gctggaggaa
      121 ttcctgcaag ggtcaggggg ccggtgtgcc gggagcgatc tgttcgacga acttatactg
      181 gccgttttcg attttgagca ccaccgccgg ctttaccgca ttgcggttgg cgtcgatggt
      241 gatttggccg gaaacgccgg agaaatcttt cgtttcggcc aggacccggc gaacggcgtc
      301 gccgtcggtg gagttcgctt tctggatggc ggcaaagaga atgcgggcgg cgtcgtaggc
      361 cagaggcgct atggcgtccg gcgcctggcc gtacttctgc ttgtaggtgg cgacgaagtt
      421 ttggacttcc gaacgcgggt cgtcgctgga gtagtgagtg gagaagtaac agttgtcgag
      481 cgccgcaccc cccaccttga gcaggtcggg cgactcccag ccgtcgccgc cggtaatggg
      541 gacggtaatc cccagctccc gcgcctggcg ggcaatcagg ccgacttcgg tgtagtagcc
      601 gggcacgaaa atcgtttccg gccgtttggt tttgatggtg gtcaattgcg ctttgaaatc
      661 ctgatcgcct tcggtgtagg cttcttcggc gaccacctgg ccgcctgact ctttgaaggt
      721 tttcgcaaag tactcggata atcccacgct gtagtcattc ttcacgtctt tcaagatagc
      781 cacccttttc attttgagcg tattgcggca gaatttagcc atcacttcgc cctggaacgg
      841 gtcgatgaag caaacgcgga aaatatagtc gccaatctgg gtgaccgcgg ggttggtgga
      901 agaggggctg accatcggaa tcttattctg ctggcagatg cggccgcccg ccatgctccg
      961 ggtgctggcc acctccccca gcaccgccac cactttgtcc tggctgatca acttctgaac
     1021 tgcagtggcc gcctcttccg gcttcccctg gtcatcctcc acgatgatct gcactttctt
     1081 ccccaaaagt ccgcctccgg cattgagctc ttcggtcgcc atgtcgatgc cgttcttggt
     1141 ggaaatgccg aatgtggcgg tggtgccggt gagggagccg tattcaccga ctttgatgac
     1201 gtttccctgt ttggcgcaag tgctgaaggt tcccaggatg aaaagagaag ccaataccca
     1261 accaaacgat ttccgcattt ttcctccctc gttggccaat gaatgccgcc tacgcatgag
     1321 tgccccttaa tttgtggggt ttcgaccgtt tgtcaagtcg agatagaacc gcactgattt
     1381 cataattgac aatccttttg ccggcccgta aaatgtttcc gcgatggggg agaggcggca
     1441 cagcggtcgg gggtcgggat gagtacccaa attcttcttg ttcttctcac cagcgttttg
     1501 gcgggcgggg ccaacgtcat cggcggactg atcgtggcct cccgggcggg ctggacgaag
     1561 caattgctcc gctactgctt agctttcggc gcagggttca tcttagccgc agccgcgctg
     1621 cggatgattc cgatttcaat ccaggcccgg ccggatgcgg cgcttctggt tctggctggc
     1681 tacttcttcg tccacctgtt cgagcatacg gtttcgccgc atttccactt cggtgaagaa
     1741 acccacgcgg tggaagccgg tttgacgcgg caggtcggct tctctgcggt cttcgggctg
     1801 gtcatgcacg cctttttcga cggggtgacc atctcttcgg gttttgccgt caccccggct
     1861 ctgggattgc ttttgttttt ggccatcctc ctgcacaagc tgccggaagg attttcggtg
     1921 gcctccctgg ctttggcctc cggccattcc cgccgctggg cggtgggcgc cgctgccgtc
     1981 gtcggtattt cgaccgtcgt aggaggcgtc gccgcctctt ttctatccag tcttatgacc
     2041 tacgctctgg ctttttcggc gggcgtggca atctacgtgg cggcaaccga tttgatcccc
     2101 gaggtgaacc gcgagccggg tatcaaaatg tcgtttatgg tcttcctggg ggtggcgctg
     2161 ttctacatca ccgaacggct cctggaaatg ttaggggtat aaaaaatcag aaccgccctg
     2221 aagggcggtt ctaagaaatc tttgatcaga cttccctgcg ggggcggctc aagctgctct
     2281 tcctcctcgc tctctgacta cggcgcgcag ggcaggggat cgccaaagac aacgtggttc
     2341 aaaagagtca cgagatcctg catatcgtac gcggtatcgc aattcacgtc gccgacctgg
     2401 agaatcggtt ttggggctgg gtttcccgac agctcatact gtaagagcca gaccagatcg
     2461 agcaagtcca ccacgccgtc gtcactcacg tcacccaaaa gataggtcgg gcggccagta
     2521 accagccagt tcaggacgtc gctcaggaac tgctcgcggg tctcatatcc ctgggcggtg
     2581 cgcgtgttgg ttattccctc aaatccgaat cccaggaaaa cgacccggta attggcgcca
     2641 taccggacac cggccgctcg gccggtgctc atgtcggtga gaatggccgg cccctgcggc
     2701 aacggagaga aatagtcggg agaaggcagg ttgacggcac cgtccgcacc catgatccac
     2761 acttttttgc tgtcggtgat cggatcgccg ggctttccca ccgtataaag cgaggaggtt
     2821 atgggattgt ttccggcata acgcgtgtgg aggtaattcg tcaggaaagt cgagtcggga
     2881 ccggcggcca gcccttcggc gaggctctgg ccggagagga aaagttgccc accgttatcc
     2941 agaaaggtct ggagtttcag gacgctctgg tgggccagcg cgggcaaacg ctggtcgccg
     3001 gtgaaccaga acgcggtggg gtaactggca aaatcagcgg tgctgaccag agaggccgtg
     3061 cggaagccgt acggctctcg cagcgaatca agggcgccgg tgtaataact ttcatacgat
     3121 ttctcggagt catcatcgac gatgagcacc cgcggccgcc ccagcgtgcc tttgaccgtg
     3181 gcctggatca cggatagact gtccgcggtt gccacctcca ggctgaagta aacggtgcgc
     3241 gggagcggca gggtgggggc cagagagaag acaatcgggt ccgcgctgtt ggagacgcta
     3301 tcgagtgacg cgatcgtgcc caaggaactg gtacggtcgg aaaacaaaat atccgcatta
     3361 tcagcctgag ccacaacgga cacgccgctg gcagcagcca tgagactcgt caccgtgagc
     3421 cggaggacca ccgtttcagc ggaatcaatg acgccgtcgc cgttgccgaa ggtagtatca
     3481 tcgagccaga acgaagcgat ggccagagcg ggacgagaga agaacacctc gatattggcg
     3541 gtcatcaagc tgtcggagtt ggaaacgttc cacaccccca cctgcgtgtg ttgattcccg
     3601 tagtcgcggc tgttggggtt ggagaggtcg tcgaaggttc ggttattggt ggcccccggg
     3661 aagggatcgg ccgcatcgcc ctgacttccg ctgttgttta actgccgaag gccatcggcc
     3721 tgctcgatgt ccaccaggcg gtggttttca tcccagttac cggggacggt ttcgtcaatg
     3781 tggtagatga gtagtccatc gccgggcaag aaccggtcaa agagctttct ctgccggttt
     3841 tcgacaagga agtactgggg ccctattgct cctcctcgcc aaagacgaaa aacctgaggg
     3901 ttatcttcta cccgggctaa ggtctggctg tcggcgttgg cgacgtagtt cgtgggagta
     3961 acccatccca aagtgacttt gcaccaggcg tcgaactggg ccgggacccg gctgccgcca
     4021 ttgtaactgc cgccgaccat caccgaccag tcgccgatgc cgttggtgga gtagtcgatg
     4081 tcgtacagat cgg
//
LOCUS       DBNGZD010000336         3108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_123826, whole genome shotgun sequence.
ACCESSION   DBNGZD010000336 DBNGZD010000000
VERSION     DBNGZD010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_123826"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..659)
                     /locus_tag="AC16GM_09005"
     CDS             complement(3..659)
                     /locus_tag="AC16GM_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR2923848.1"
                     /translation="MGQATTSQENYLKTIWELTRQGLVPHGSRVASELGVSPPAVSLA
                     LRRLAREGYVAISGHGNVRLTPKGTKAAEILVRRHRLVEKLLTDVLGMPWHRVHEEAE
                     KLEHAISPEFEKRLGALFAREKTCPHGYPLAVKNSGPAAWAKLKLLSQAKAGERVTIA
                     RVYEKDSAFLLYLDRLAILPGGRAEVVDIAYDETITLRIGQRRVLLGKAAAAKIWVRA
                     "
     gene            801..2090
                     /locus_tag="AC16GM_09010"
     CDS             801..2090
                     /locus_tag="AC16GM_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR2923849.1"
                     /translation="MPETAKEIERRIRDESPPRRSFFREALQNWKIVGYLGPAFLVSV
                     GYMDPGNWGTDIQAGSDFNYNLLWVLLLSNLMAILLQTLAAKLGLVTGRTLAQNCRDH
                     YPRWLNIFLWVLTEAAMMATDLAEFLGAAIGFYILFRIPMFSAGLLTGGVVFLILALY
                     RFGYRKVEFAIIGLVAVIGFCYVIELFLAKPNLGQLAYHTVVPWINDKSILVAVGMLG
                     ATVMPHNLFLHSGVIQYRLQNGANSLKKKLFRFAVIDSMLALNLAFLVNAAILIMSAA
                     VFHRNGVPVSSIQEAHRTLIPLLGGFSSVAFAVALLSSGLSSSTTGTLAGQIVIEGFL
                     NVKMGLWLRRLITMIPALVVIALGVNELKVLVLSQVTLSLALPFATIPLIWFTTRKKL
                     MGEFANKPVTNVLAIGAASIIIFLNGLLLYQVLGGSF"
     gene            complement(2113..2778)
                     /locus_tag="AC16GM_09015"
     CDS             complement(2113..2778)
                     /locus_tag="AC16GM_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923850.1"
                     /translation="MKHPVGIILGGCGVTEGSDPYEVAFCAYHIERHGWAALFFAPPE
                     ASSTEKKNPAVWSAAQQLSRGELLALDAPPSENLTALVIPGGEGILGTLATTFHDGNE
                     IDIEPNLKRLIRDLHRRKRPIAACGVAGALVVASLFHPESLSPTVAVGKDTRWIGLVE
                     TLGGVPVQVRSDEVVIDEKNRIVTTPGFAADEQLQMVERGIKNLIDSVGQWMPKGQPM
                     VAL"
     gene            complement(2944..>3108)
                     /locus_tag="AC16GM_09020"
     CDS             complement(2944..>3108)
                     /locus_tag="AC16GM_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR2923851.1"
                     /translation="SVIVRQRGTRIYPGLNVGMGKDNTLFARITGTVHFETKGGDKKM
                     VRVEPLPQTA"
BASE COUNT          617 a          974 c          818 g          699 t
ORIGIN      
        1 ccctacgctc gcacccagat cttggctgcc gctgccttcc ccaagagcac ccgccgttgc
       61 ccaatccgca aagtgatggt ctcgtcgtat gcaatgtcca ctacctccgc cctccctccg
      121 ggtaaaattg ctaagcgatc gaggtacaag agaaaggcac tgtccttctc ataaactcgg
      181 gcgatagtca cccgctcgcc cgctttggct tgagagagga gcttcagctt cgcccaggcg
      241 gccgggccgg agtttttcac ggctaaagga tagccatgcg ggcaggtctt ctccctggca
      301 aaaagcgctc ccagccgttt ttcaaattct ggcgaaattg cgtgctccaa cttctccgct
      361 tcctcgtgga cccggtgcca gggcatgccc agtacgtcgg tgagcaactt ctccaccagc
      421 cggtgccgcc gcaccagaat ttccgccgct ttggtaccct tgggcgtcag gcggacgttt
      481 ccgtgaccgg aaattgcgac gtacccttcc cgggccagcc gccggagggc caaagacacc
      541 gccggcgggg aaacccccag ttcgctcgcc acccggctgc cgtggggtac cagcccctgc
      601 cgggtcaact cccagatggt cttgaggtag ttctcctgac tcgtggtcgc ctggcccata
      661 gcaaaaaaat ccctaaaaat agtgcttgac agcgccgata ttaacgtata ttaatggata
      721 atattaagca tagttaatat cggtctcccg gttagagaaa agatacaacc gggacccaca
      781 actgacaagt gctacagccg atgccggaaa ctgccaaaga aatagagcgc cggattcgcg
      841 acgagtcgcc gccccggcgc agcttcttcc gggaagcgct ccagaactgg aagattgtcg
      901 ggtacttagg gcccgccttt ctggtgtcgg tggggtatat ggaccccggc aactggggta
      961 ccgacatcca ggccggctcg gattttaact acaacctcct ctgggtgctc ctgctctcca
     1021 acctgatggc cattctcctg cagacgctcg ccgccaaact cggcttggtc accggaagaa
     1081 cattagcgca aaactgccgc gaccactacc cgcgctggct caatatcttt ctctgggtat
     1141 tgaccgaagc tgctatgatg gccaccgact tagcggaatt tttgggcgct gctatcggct
     1201 tttacatcct gtttagaatt ccgatgttct ccgcagggct tttgaccggc ggggtggttt
     1261 tcctcattct ggcgctctac cgcttcggct accgcaaggt ggaattcgcc atcatcggcc
     1321 tggtggcggt gatcgggttc tgctacgtca ttgaactctt cttagccaag cccaacctcg
     1381 gtcagcttgc ctaccacacg gtggtcccgt ggatcaacga caagagcatc ctggtcgccg
     1441 tcggcatgct gggcgccacg gtgatgcccc acaacctctt cttgcactcc ggcgtcatac
     1501 agtaccgcct gcagaacggc gccaactcgc tcaagaagaa actgttccgt ttcgcggtga
     1561 ttgattcgat gctggcactc aatctggctt ttctcgtgaa tgccgctatt ctcattatgt
     1621 cagcggcggt tttccacagg aatggcgtgc cggtgtcatc gatccaagaa gcgcaccgca
     1681 cactcattcc gctcctgggc ggattttcca gcgttgcctt tgccgtcgcc ctgctctcct
     1741 cgggactgtc ttcatcaacc accggcactc tcgccggtca aatcgtgatc gagggctttt
     1801 tgaacgtcaa aatgggcctc tggctcaggc gcctgatcac gatgattccg gctctcgtcg
     1861 tgatcgcttt gggcgtaaac gaactgaagg tgctggtttt gagtcaggtg accttgagct
     1921 tggcgcttcc cttcgccacg attcccctaa tctggttcac cacccggaaa aaactgatgg
     1981 gcgagttcgc caacaagccg gtcaccaacg tcctggctat cggcgccgcc agcattatca
     2041 tctttctgaa cggacttctg ctctaccagg ttctgggcgg gtcgttctaa tccggcggca
     2101 gttcctgttc tttcataacg cgaccatagg ttgccccttc ggcatccact gcccgactga
     2161 atcaatgagg ttcttaatcc cccgttccac catttgcaat tgttcatccg ccgcaaagcc
     2221 gggcgtggtg acgatgcggt tcttttcgtc gatcaccacc tcgtccgacc gcacctgcac
     2281 cggcacgccg cccagagttt ccaccagccc gatccagcgg gtgtccttgc ccaccgccac
     2341 cgtcggggag agtgattcgg gatggaaaag ggacgcgacg acgagcgccc cggccacgcc
     2401 gcaagcggca atcggccgct tccgccggtg gaggtcgcgg atgagccgct ttaaattcgg
     2461 ttcaatgtca atttcattac cgtcatggaa ggttgtcgcc agcgttccta aaatcccttc
     2521 gccgccggga atgaccagag cggtcaggtt ctcactggga ggtgcatcga gagccaagag
     2581 ttcaccgcgg gaaagctgct gggccgccga ccacaccgct ggatttttct tttctgttga
     2641 agacgcttct ggaggagcaa aaaaaagtgc cgcccagccg tgccgctcga tgtgataggc
     2701 gcaaaaggcg acctcgtacg ggtcgctccc ttcggtgacc ccgcaaccgc ccagaatgat
     2761 gcctaccgga tgtttcatgg ttcaacgaaa cataagcagg gagacccaaa agaaaaaccc
     2821 cgattttcca atcggggaac gcccgccctt aatacatcct catcgtagga gcagacccct
     2881 gtgtctgccc atccttttca atggatacac ctgtaggggt ttgtttaatc aaacccctac
     2941 accctacgct gtttgcggca gcggctccac gcggaccatt ttcttatccc cgcctttggt
     3001 ctcgaaatgg acggtgccgg ttatccgggc aaaaagtgtg ttgtccttcc ccatgccgac
     3061 attcagtccg gggtaaatcc gcgtcccgcg ctggcggaca ataaccga
//
LOCUS       DBNGZD010000337         3029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_54234, whole genome shotgun sequence.
ACCESSION   DBNGZD010000337 DBNGZD010000000
VERSION     DBNGZD010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_54234"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="AC16GM_09025"
     CDS             complement(<1..1378)
                     /locus_tag="AC16GM_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923852.1"
                     /translation="MKIGRRALGIGGLWLLAAVGVWAAPPSQKATPPLAKPGAPQIPD
                     NSQVIDINNLLMWVTNHGSFAWDIQSGDAGLQFPKPAPGQPPKTVVFASGLWVGGKVR
                     DTIRVALGEYSQEYVPGPMAQPCTPAGLDGPGCFLPDNANFRVFKVLRNNPTALDWPN
                     WIIAARQGAPFVGTDSNTPAVLGDQMLWAVYNDASPAIHTNTAGSSKPLGIEVQQTTF
                     AFNRTGALGQTIFLKFKVINKGQNTIDSCYLSLWADPDLGGFTDDLVGCDTTLSLGFC
                     YNATNTDEQYGSTPPSVGYDFFQGPIGDLGQRLPMTSFNKYINGTDPASKDETYNYMK
                     GLQPDGSPLINEVTGDTTTFFVSGDPVAGTGWLDNSPADRRMQLTSGPFTFAPGDTQE
                     VVAAIIVGQGGDRLSSITVLKFFDKSAQKAFDLNFVLPSPPDAPAVTVHRLDSKIQLT
                     WTDRSEIQH"
     gene            complement(1418..>3029)
                     /locus_tag="AC16GM_09030"
     CDS             complement(1418..>3029)
                     /locus_tag="AC16GM_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR2923853.1"
                     /translation="YGRTYGNPNFNINGPLGTIFWPGDDPSTPIVRNAEGIITKDTLG
                     RNTDESSLFDDFLKQRSWYIGGAFNVASQVTEHHLVQGGADFQRHTLRRYHHLYPANV
                     GLDNKATRYVDADYFGYDSAFTKEVNGGDNGAKHPLLFSVYLQDKMETEQAIVNLGLR
                     YDYLSSQTKLLADPSRPFLGAPSVYTCQGCLVANKARHRVSPRLGVAFPIAERTQFHI
                     NYGKFFQLPNLEDLYVGLPYLNYKVPLAGYYFAFGNPNLLPEHTTAYEVGVTQGLTEN
                     SRVDVTAYFKDVSGLVEVANIESNPSNFASFRNNDFGTIKGVDISYKTRRTRALSLEV
                     NYSYSQATGTGSTSQTQRNIAWTATEPPKLTHPLDFDQRHKISMIADLRAEKGQGPKV
                     GNYHILENAGINVLLNAGSGLPYTPAKPFDAVTLAAVSLQPSGPINSSYRPWTFRIDL
                     KATKKFSYAGADLEFYVTILNLTDRKNALTVYPTSGSPTSTGFLSTETGAATYDTPAK
                     QALYHLAEQNPSSFDVPRMVRFGTRLSF"
BASE COUNT          602 a          807 c          958 g          662 t
ORIGIN      
        1 cgtgctggat ctccgaccgg tcggtccagg tcaactgaat cttgctgtcc aaccggtgca
       61 ccgtcaccgc cggagcatcc ggtggcgatg gcaggacaaa gttcaagtca aaagccttct
      121 gggcggattt gtcgaagaat ttcagtactg taatggaact gagccggtcg ccgccctgcc
      181 cgacgatgat ggctgccacg acttcctgcg tgtcgcccgg ggcaaaggta aagggcccgg
      241 aagtaagttg cattcggcgg tcagcgggag aattatccag ccagccggtg ccagctaccg
      301 gatcaccgga aacgaaaaag gtcgtcgtat cgccggtcac ctcgttgatg agcggacttc
      361 catccggctg caaccctttc atgtagttgt aggtttcgtc tttggaagcc gggtcggtac
      421 cgttgatgta cttgttgaag gaggtcatcg gcaggcgctg gcctaaatct ccgatggggc
      481 cttggaagaa gtcgtagccg accgaaggcg gcgttgaacc gtactgttcg tccgtgttag
      541 tggcgttgta gcagaaaccc agagacaaag tggtgtcgca gcccaccaga tcgtcggtaa
      601 agccgcccag atcgggatcg gcccagaggg agaggtagca ggagtcgatg gtattctgcc
      661 ccttgttgat caccttgaac ttcaaaaaaa ttgtctgccc caaggcaccg gtgcggttga
      721 aggcaaaagt cgtctgctgc acttcgatgc ccaacggctt agacgatcca gcggtgttgg
      781 tgtgaatggc cggagaggcg tcattgtaca ccgcccaaag catctggtct ccgagaactg
      841 cgggagtgtt gctgtcggtg cccacaaagg gagctccctg tcgggcagcg atgatccagt
      901 tcggccaatc cagggctgtg gggttgttgc gcagcacctt gaagacccgg aaattggcgt
      961 tgtccggcag aaagcagccg gggccatcca gccccgcagg agtgcagggc tgggccatcg
     1021 gcccggggac gtactcctgc gaatactcac ccagggccac ccggatcgta tcgcgcactt
     1081 ttcccccgac ccagagcccg gaggcaaaca ccacagtctt gggtggttgc ccgggagctg
     1141 gctttggaaa ctgtagcccg gcatcacccg attgaatgtc ccaggcaaac gagccgtgat
     1201 tggtgaccca catcaaaagg ttgttgatgt caatcacttg gctgttgtcg gggatttgcg
     1261 gcgctccggg cttggccaga ggtggtgttg ctttttgact tggcggtgcc gcccagaccc
     1321 ccaccgcggc cagaagccaa aggccgccta tgcccaaggc gcgtcttccg attttcatag
     1381 gttccctcct caaggtcaaa ctgctgtttc tctccggtta gaatgacaag cgggtgccaa
     1441 aacgcaccat ccgcggcacg tcaaagctcg aggggttctg ttctgccagg tggtagagcg
     1501 cctgtttggc cggcgtgtcg taggttgcgg ctccggtttc ggtggagaga aaaccagtcg
     1561 atgtaggtga gccgctggtc gggtagacgg tgagcgcgtt tttccgatcg gtcaggttca
     1621 gaatcgtcac gtagaactcc agatccgccc cggcgtagga aaatttctta gtggctttga
     1681 ggtcgatccg gaaagtccag ggccggtaac tggagttgat cggcccggat ggttggagcg
     1741 acaccgccgc caaggtcacc gcatcgaacg gcttggccgg agtgtagggc aaccccgagc
     1801 cggcattcaa aagcacgttg atgccggcat tctccaggat gtggtagttg cccaccttcg
     1861 gcccctgccc tttttccgcc cgcaggtcgg caatcatgga aattttgtgc cgctggtcga
     1921 aatcgagggg gtgggtcagt ttcggcggct cggtggcggt ccaggcaatg ttcctctggg
     1981 tctgagaggt ggaaccggtg ccggtggctt gagagtatga gtagtttact tccagagaca
     2041 gtgcccgggt ccgccgtgtc ttatatgaga tgtccactcc tttgatggtc ccgaagtcgt
     2101 tgttgcggaa cgaggcgaag tttgaaggat tggactcgat gttggccact tccaccagac
     2161 cggagacatc tttgaagtag gcggtcacat cgaccctgga gttctccgtc aatccctgag
     2221 tgacacccac ctcgtacgca gtcgtgtgct ccggtaacag gttggggttc ccaaaggcga
     2281 agtagtaacc ggccagagga accttgtaat tcagataggg taagccgacg tagaggtcct
     2341 ctaagttcgg caactggaag aacttcccgt aattgatgtg gaactgtgtc cgttccgcta
     2401 tgggaaacgc aacccccagc cggggactta cccggtgccg cgctttgtta gcaaccaggc
     2461 aaccctggca ggtgtagacg ctgggtgcgc cgaggaacgg tcgggaagga tccgccagta
     2521 gcttcgtttg agacgagagg tagtcgtagc gcaagccgag atttacgatg gcctgctcgg
     2581 tttccatttt gtcctgcagg tagactgaga agagcagggg atgtttggcg ccgttatcgc
     2641 cgccatttac ctctttggtg aaggccgagt cgtacccaaa gtagtcggca tccacgtagc
     2701 gggttgcttt attgtccaga cccacgttgg cagggtagag gtggtggtag cgccgcaggg
     2761 tgtggcgctg gaaatcagcg ccgccttgca ccaggtggtg ctcggttacc tgcgaggcga
     2821 cgttgaacgc accgcctata taccaggaac gctgcttcag gaaatcgtca aagagcgacg
     2881 attcatccgt gtttcgaccc agagtatcct tggtgatgat gccttcggcg ttgcgcacga
     2941 tcggagtact ggggtcatct ccgggccaga atatggttcc taacggcccg ttgatgttga
     3001 agttggggtt cccgtaggtg cggccgtat
//
LOCUS       DBNGZD010000338         3043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1374396, whole genome shotgun sequence.
ACCESSION   DBNGZD010000338 DBNGZD010000000
VERSION     DBNGZD010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1374396"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1246)
                     /locus_tag="AC16GM_09035"
     CDS             complement(212..1246)
                     /locus_tag="AC16GM_09035"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR2923854.1"
                     /translation="MTLSDLEHFKNLLIEREQNLTAWLDSSGSPNGEDVQKAQALLMD
                     IRQALGRVENQTFGACQGSAPAGICKGEVELYRLEVQPVTEVCLGCITKEEQARLEEE
                     LFLASKIHRALLPQSIERIEGYDVGVKALAARSVGGDYYDFLPSANGGRLRVVIADTM
                     GKGIPAGLLMSHVQGALKILSEDIESPRLLISRLNQWLCRNVPVTKFVSLSCLALDSR
                     AGRDSQITYTNAGHCPPILVRNDGKVERLEPTGGVLGVHEGFTYEEHTLSLSPGDLLV
                     LYTDGVTEAENYHGGMFGEERLIEFAQTRRTDPLDKFLDDLLGEVQFFSEKPEMDDDF
                     TVIALRKTAS"
     gene            complement(1439..1591)
                     /locus_tag="AC16GM_09040"
     CDS             complement(1439..1591)
                     /locus_tag="AC16GM_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923855.1"
                     /translation="MSMSDIILVGALIGCVWMFMNQHKKEKAKKVYRPTRLPQPPPRP
                     RTPVNY"
     gene            complement(1733..1966)
                     /locus_tag="AC16GM_09045"
     CDS             complement(1733..1966)
                     /locus_tag="AC16GM_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR2923856.1"
                     /translation="MTKLPVLSGSELVKILQRAGFEKVRQKGSHVSLRKKTFERVFHT
                     VVPMHREIDRGTLAEILRQAGLSRDDLQQLMET"
     gene            complement(2115..2360)
                     /locus_tag="AC16GM_09050"
     CDS             complement(2115..2360)
                     /locus_tag="AC16GM_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR2923857.1"
                     /translation="MRKNNKHSPSYKFTAVIVRGEKLYVARCLEVDVVSQGRTIEAAK
                     ENLKEAVELYIESFGLPQPKGKTAESFVTPLEISLTK"
     gene            complement(2473..2589)
                     /gene="rrf"
                     /locus_tag="AC16GM_09055"
     rRNA            complement(2473..2589)
                     /gene="rrf"
                     /locus_tag="AC16GM_09055"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2892..>3043)
                     /locus_tag="AC16GM_09060"
     rRNA            complement(2892..>3043)
                     /locus_tag="AC16GM_09060"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          691 a          844 c          809 g          699 t
ORIGIN      
        1 ccaaaaataa agcgaataaa aaaatccctc ccctttgagg ggagggtagg gaggggtcaa
       61 agcaacgccc attaagggct aggctacgaa ctgacctctc cccggccctc tcctcaaagg
      121 agagggagaa aattgcccgg acttactctg gtaaaatgac aggtgctttt ccctcccctt
      181 ggaggggagg gtagggaggg gtcatatttt actatgaagc ggtcttccgc agggcaatca
      241 ccgtaaaatc gtcatccatc tccggtttct cggagaagaa ctggacttcg cccagaaggt
      301 cgtcgaggaa cttatcgagc ggatcggtgc ggcgggtctg ggcgaattcg ataagccgct
      361 cttcgccgaa catccccccg tggtagttct cggcctcggt gacgccgtcc gtgtagagca
      421 ccaaaagatc accgggagaa agcgacaatg tgtgctcttc gtacgtaaag ccctcgtgta
      481 cgcctaaaac cccgccggtc ggctccagac gctcaacctt accatcattt cgcactaaaa
      541 tcggcggaca gtggccggca ttggtgtagg tgatctgaga atcccggccg gcgcgggaat
      601 ccaacgcgag gcagcttaaa gagacaaact tggtgacggg gacgttgcgg cagagccact
      661 ggttcaggcg gctgatgagc aggcggggcg actcgatgtc ttcggataaa attttaagcg
      721 cgccctggac atgcgacatt aaaagcccgg ccgggatgcc tttgcccatg gtgtcggcaa
      781 tgactacccg caagcggccg ccgttggccg aaggcagaaa atcgtagtag tcgccgccca
      841 cgctccgggc ggccaaggcc ttcaccccca cgtcatagcc ctcaattctt tcgatcgact
      901 gcggcaggag tgcccggtgg attttgctgg ccaaaaacag ttcctcttct aaccgtgcct
      961 gttcctcttt ggtgatgcaa cccaggcaga cctcggtcac cggctgcacc tccagccggt
     1021 agagttccac ttccccctta caaattcccg ccggagcgct tccctgacag gcgccgaacg
     1081 tctggttttc gacgcgcccc aaggcctggc ggatatccat caaaagcgcc tgcgccttct
     1141 ggacgtcctc cccgttcggc gaaccggagg agtcgagcca ggcggtcaag ttttgctcgc
     1201 gttcaatcaa gagatttttg aagtgttcga gatctgagag cgtcatggtc gcgtgtccta
     1261 ataaaccgtc tattttctgt acggaaattg cagggggaag ttttcaaaaa caaaaagccc
     1321 cgccagtcga aagagccgtc atacgaatga cgacccttcg gcaagcggga caaaactctt
     1381 agtgaacgcc tattgccgat gcgacaaact tgcccctgcg agcgaactca agggaaacct
     1441 aatagttgac cggtgtcctc ggccgggggg gcggctgggg caggcgggta ggccggtaca
     1501 ccttcttggc tttttctttt ttgtgctggt tcataaacat ccacacgcac ccgatgagtg
     1561 cacccacgag aatgatgtcc gacatgctca tagcgcctcc ttcataggtt tcatctacta
     1621 tatcggcgga ttgggggaaa catccacagg gaggacttga ctatgccata tcatcctccc
     1681 cttgaggggg gtagggaggg gtcacgcaca caggactctt tagaggggaa cactatgttt
     1741 ccatcaattg ctgtaaatcg tctcgggaca aaccggcctg cctcagtatc tccgccaacg
     1801 tacctcggtc aatctcgcgg tgcataggaa cgacggtatg gaatacgcgt tcaaaggtct
     1861 tcttccgcag cgaaacatgg ctcccttttt ggcgaacttt ttcaaacccc gctcgctgga
     1921 ggatttttac aagttcggaa ccagaaagga ctggaagttt ggtcattccc cgaaagaggt
     1981 aggaacatgg ttaaattgtc aagtcgtttt agggattttt gaaaaaaata gactattagt
     2041 ggactgccgg taagaagttt tttaagaagc tatacatttg accagtatct tgatatcggg
     2101 acgaacggga aggactactt ggtgagagat atttccaagg gtgttacgaa cgactcggcg
     2161 gttttccctt ttggctgtgg caacccgaag ctttcgatat acaactcaac ggcctctttc
     2221 aggttttctt tggccgcctc aatcgtccgg ccctgagaga ccacgtcaac ctccagacag
     2281 cgggcgacgt acagcttctc accgcgcaca ataaccgcag tgaatttata cgaaggtgaa
     2341 tgcttattat tctttctcat agctcccaag ttaccttttc gtcacattct gtcaaattct
     2401 ttgctctatt ttatgcaact cccaatataa tcaaaagccc cgattcttgt cgaatcgagg
     2461 cttggatttt taacccggcg gcgacctact ctcccacacg gtcacccgag cagtaccatc
     2521 ggcgctgagg ggcttaacga ctgtgttcgg aatgggaaca ggtgtttccc cctcgctatg
     2581 gccgccggga aattcaattc ctgattgcga gtcgcgccgg ccttactgtc cggtcggtgg
     2641 accgcctggt gttttctttt tgcgcgccaa gaccacgatg atgataacca caaccacgat
     2701 gagaatgacc caaccgatgg tacccatctc ccctcctctg ttcgggtttg cctgtgcctc
     2761 acccccgcct ttggcggggc cacctgcgcc tccatcgctg caggagtgca ttcacagaaa
     2821 acaaccagga ctccaaatct ttagttaccg cggcgggcaa gccctgtgca aaaagcacga
     2881 tcagagcaat atggatcaag tcgcacggcc gattagtacc ggttagctgc gcggcttgca
     2941 ccgcttccac acccggccta tcgacgtggt ggtctaccac ggctctgatg gggagaactg
     3001 gtttcgaggg gggtttcccg cttagatgct ttcagcggtt atc
//
LOCUS       DBNGZD010000339         3665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1652038, whole genome shotgun sequence.
ACCESSION   DBNGZD010000339 DBNGZD010000000
VERSION     DBNGZD010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1652038"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..983
                     /locus_tag="AC16GM_09065"
     CDS             18..983
                     /locus_tag="AC16GM_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR2923858.1"
                     /translation="MGEPPTTEVQVYEEHATSILSRNDSPDLGFRFSVNPYRGCFHGC
                     AYCYARPTHEYLGFGAGSDFESKIVVKTNAAELLRQEFLKPSWKGELIFFSGVTDCYQ
                     PQEAVWELTRKCLEVCLEFRNPVSIVTKSYLIVRDLDLLKELHQEAFADVHFSIPFSR
                     DDIAKKIEPNAPSITKRFEALEKLSQAGIPTGVLIAPLIPGLNEQDMPEILKRASACG
                     AKNAGWVLLRLPGSVKDVFLKRIKEQFPDRAEKIINRIKSVRGGVLYNSEFFERQRGK
                     GHYWKIVEEMFRVYQKRYGLDQEVDYLTSTTFRVPTRQVELALSA"
     gene            complement(934..1518)
                     /locus_tag="AC16GM_09070"
     CDS             complement(934..1518)
                     /locus_tag="AC16GM_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923859.1"
                     /translation="MENLEKYKQYYFLENYLFDTVTKNFERNRHLTEEEFFAIVIWKS
                     NRAKTKVRDGIKESQKTVQMITSEISQAKTPEEKLKLLTDIDGIGLPMASAILTVCYP
                     KDFTVVDYRARSSLKEFDVEIKGDPSTDCTAYFDYLNKCMKLAQKFNFSLRDFDRILW
                     AKDFCEGKEGLKDLVKSLTTQKAPVPLAWSAPGK"
     gene            complement(1573..1656)
                     /locus_tag="AC16GM_09075"
     tRNA            complement(1573..1656)
                     /locus_tag="AC16GM_09075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1620..1622),aa:Leu,seq:caa)
     gene            1832..2395
                     /locus_tag="AC16GM_09080"
     CDS             1832..2395
                     /locus_tag="AC16GM_09080"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR2923860.1"
                     /translation="MTKPQGPFFTLPNLLSLSRLLLVPVFILLAFQEGASVRWLLAAV
                     IIYGITSDIADGLIARYTKQVSDWGKILDPLSDKISTATVAWYVWLRHDFPLWAFILL
                     LVKDIAILVAGTVTISKSTAIPVSNFWGKTAALLWAVTVLAYFLDLRSMKEGFLYAAT
                     AATIPALVSYGKRWQRQKKEALAGVIR"
     gene            2350..>3665
                     /locus_tag="AC16GM_09085"
     CDS             2350..>3665
                     /locus_tag="AC16GM_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR2923861.1"
                     /translation="MAASEEGSAGGSDTLMLRRLAIKNYAIIDDVTVEFTPSFTVLTG
                     ATGAGKSIIIGALNLLLGEKASSEVVRSGETNAWVEGEFEIERSEPVTSLFSEAGIET
                     DATLLIRREVADRGTSRALVNDRQVTVGFLKRLGDFLVDLHGQHQHQWLLAAERHLDF
                     LDGFCGTLELREKMGELYNSYVTAHRKLRDLEKLQKESGEREELFRFQVTEIRKANLS
                     IAEEETFLAEKKRLDHAQELVQSAERLYDSLYDSEGSATERLKLAVRELSRLGAIDAD
                     LAKQAESLEQAHIVTQDVASFLGQYRERVGFDPERYQQVVDRLDLYFRLKKKYGGSVD
                     AVNALADDLEKQLHQRMNLGEEIKQTTAELARLRGECARVATALSTKRTEGAGALAKR
                     VEKELEQLGMAKTKFQVQIVQSESDDGIVVNGKTRALSETGIDQVE"
BASE COUNT          868 a         1011 c          935 g          851 t
ORIGIN      
        1 gattctccta cgactggctg ggagaaccgc cgacaacgga ggtgcaggtc tacgaagagc
       61 acgccacgag catcctttcg cgcaatgaca gccccgatct gggcttccgc tttagcgtca
      121 acccctaccg tggctgcttc cacggctgcg cctactgcta cgcccgcccg actcacgagt
      181 atctcggctt cggcgcgggg agtgatttcg aatccaaaat cgtcgtcaag accaatgccg
      241 ccgaactcct ccggcaggaa ttcctcaagc cctcctggaa aggggaactg atcttctttt
      301 ccggcgtcac cgactgctac cagccccagg aggcggtctg ggaactgacc cgcaagtgtc
      361 tggaggtctg cctggaattc cgcaatccgg tctccatcgt caccaagtcg tacctcatag
      421 tccgcgacct tgacctgctc aaagaattgc accaggaggc ctttgccgac gtgcatttca
      481 gcatcccctt cagccgagat gatatcgcca aaaagattga gccgaacgca ccgtcgatta
      541 ccaaacggtt tgaagcgctg gaaaaactct cgcaggccgg gattcccacc ggcgttctca
      601 ttgccccgct catccccgga ttgaacgaac aggatatgcc ggagattttg aagcgcgcca
      661 gtgcctgcgg cgcgaaaaat gccgggtggg tgttgctccg cctgccggga agcgtcaaag
      721 acgtcttcct gaagcgcatc aaagagcagt ttcccgaccg cgctgaaaag atcatcaacc
      781 gcattaaatc agttcgcggc ggcgtactct acaattccga attcttcgag cgccaacggg
      841 gaaaggggca ctactggaag attgtggaag agatgttccg ggtctaccag aagcggtatg
      901 gcctggatca ggaagtcgat tacctcactt caactacttt ccgggtgccg accaggcaag
      961 tggaactggc gctttctgcg tagttaaact tttgactaaa tctttcaaac cttccttccc
     1021 ttcgcagaaa tctttagccc agagaattcg gtcgaaatct cgtagtgaaa aattaaattt
     1081 ctgagctaat ttcatacact tgtttagata gtcaaaatat gcagtgcaat cggtggaagg
     1141 atcgcctttt atttctacgt cgaattcttt aagtgaagat cgagcacgat agtctaccac
     1201 tgtgaaatcc tttggataac atactgtcaa aatcgctgaa gccattggaa gaccaatgcc
     1261 atcgatatcg gttagcagct taagtttttc ttcaggtgtt ttggcttggg atatctcgga
     1321 agtaatcatc tgaactgtct tttgactttc cttaatgcca tctcgaactt ttgtttttgc
     1381 cctattcgat ttccaaataa caattgcgaa aaattcttct tcagtaagat gcctatttct
     1441 ttcaaagttc ttggtcactg tgtcgaaaag atagttttca aggaaataat actgtttata
     1501 cttttccagg ttttccatga taccctcctt ctcgaaaaga ttttctttta cggttcgagt
     1561 tgatgtaact tctgccggag gtcggagtcg aaccgacatg tccttgcgga cacgggattt
     1621 tgagtcccgc gcgtctacca gtttcaccac tccggcaaaa cgaaatcaat tcctttcgat
     1681 acataatccc cgcaacgaaa taattgtttt gtaggggctc aatgcgttga gccctccaat
     1741 ttcgattttt caaatatatt gagcccccgt accttgtcaa aatcgtgcgc ctgctgtaga
     1801 tttctggcac tcggacgggt ctccgagtcc tatgaccaaa ccacaaggac cgtttttcac
     1861 tttgcccaat ttattgtccc tctcgcggct tttgctcgtg ccggttttca ttcttctggc
     1921 ttttcaggaa ggcgcatcgg ttcgctggct tctggcggcc gttatcatct acggcatcac
     1981 gagcgacatc gccgacggcc tgattgctcg ctacaccaag caagtttccg actggggcaa
     2041 aattttggat ccgctttcgg acaaaatcag caccgccacc gtggcctggt acgtctggct
     2101 ccgccacgat ttccccctct gggcgttcat tctgctatta gtcaaggaca ttgctatttt
     2161 agttgctgga acggtcacaa tctctaagag cactgcgatt ccggtctcca atttctgggg
     2221 aaaaaccgca gcgctcttat gggcggtgac ggtgctggcc tacttcctcg atttgcggtc
     2281 gatgaaagaa ggattcctct atgccgccac cgccgcaacc atccccgcgc tcgtttcgta
     2341 cgggaaaagg tggcagcgtc agaagaagga agcgctggcg ggagtgatac gctgatgctc
     2401 cgccggctgg cgataaaaaa ttacgccatc atcgacgacg tgacggttga attcacgccc
     2461 agcttcaccg tgctcaccgg cgccaccggc gcagggaagt ctatcataat cggtgccctc
     2521 aatctcctgt taggggagaa ggcgagttcc gaagtcgtcc gctccggcga aacaaacgcc
     2581 tgggtggaag gcgaattcga aatcgagcgc tccgagccgg tcacgtcgct tttttctgag
     2641 gccggcatcg aaaccgatgc aacccttctc atccggcgcg aagtggccga ccggggcacc
     2701 agccgcgctc tggtcaacga ccgacaggta accgtcggct ttcttaaacg tctcggagat
     2761 ttcctcgttg acctacacgg ccagcaccag caccagtggc ttctggctgc cgagcgccac
     2821 ctcgacttcc tcgacggctt ctgcggcact ctcgaactac gcgaaaaaat gggcgaatta
     2881 tacaactcct atgtcaccgc ccatcgaaaa ctaagagatt tggaaaagct ccagaaggag
     2941 agtggagaaa gagaggaact tttcagattt caggtcaccg aaatcagaaa agcgaatctc
     3001 tccattgccg aagaagaaac attcctggcc gaaaagaaac gcctcgacca cgcgcaggaa
     3061 ttagttcaat ctgccgaacg gctctatgac tcgctctacg actccgaggg ctcggccacc
     3121 gaacgcctga aactcgcggt gcgggaattg agccgcctcg gagcaattga cgccgacctg
     3181 gccaaacagg ccgagtctct ggagcaggcg cacatcgtta cacaggatgt ggcgtcattt
     3241 ttgggccagt accgcgagcg ggtcggcttc gatccggaac gctatcagca ggtggtggat
     3301 cggttggacc tctatttccg gctgaagaag aaatacggcg ggtcggtgga tgcggtgaac
     3361 gctctggcag acgatctgga aaaacagtta caccagcgga tgaatctcgg agaagaaatc
     3421 aaacaaacta cggcagaact cgcgcgcctg cgcggcgagt gtgcgcgggt ggcgacggca
     3481 ctctcaacca aacgcaccga aggcgccggc gctctggcca aacgcgtgga gaaagagtta
     3541 gagcagttgg gaatggcgaa gacaaagttc caggtccaga tcgtccagag cgaatccgac
     3601 gatggcattg tagtcaacgg caagacccgc gccctgtcag aaaccggcat cgatcaagta
     3661 gaatt
//
LOCUS       DBNGZD010000340         5068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_263858, whole genome shotgun sequence.
ACCESSION   DBNGZD010000340 DBNGZD010000000
VERSION     DBNGZD010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_263858"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC16GM_09090"
     CDS             <1..194
                     /locus_tag="AC16GM_09090"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR2923862.1"
                     /translation="DIVTKEPKEAIVERSDVCVVPCYGVVAEAVVALVLANAFLEKFG
                     GDSLVEIRDHFQTYNERKF"
     gene            194..1165
                     /locus_tag="AC16GM_09095"
     CDS             194..1165
                     /locus_tag="AC16GM_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923863.1"
                     /translation="MLKTTVVGNYPKISSDRSAPNLRNALNQAESGKITPQQVEEVYQ
                     STIRRVIEEQKRAGVDLITDGQIRWNDIVTPFAKKISGFEINGLLRFFDNNVYYRKPV
                     LKAKPAFNGSAAVEQFRFAQKFAGENLKAVIPGPFTFARMSVDEFFGNEEKFVLTLAE
                     LLHQEAVALVEAGATWVQIDEPCLCVEPGKIDLVRRGIEIVTRGLKAKTMLFLYFGSI
                     KPLVPKLFEFPVDAFGVDCVSRPENFSLLLTAPKGKGICCGCLDGRNIKLETDRELFP
                     YFEKLAARTDGGEVFISPSSGLEFLPHADAVQKLERMVTAVRTFNTR"
     gene            1168..2088
                     /locus_tag="AC16GM_09100"
     CDS             1168..2088
                     /locus_tag="AC16GM_09100"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2923864.1"
                     /translation="MLTDRVIKTSITIKAPAEDILTAFLDIGWMREWWGIEGGLVEPK
                     RDGLWTVVWGHSAEGYQAIGTGIVRRLGPYLLEIDSNLVFFPQRQILGPRRFQLQCRP
                     EGDKTFVDIEESGFQSGGDWDWFYESVQKGWQEVLPVLKSFLERPHARATLPSAAPVS
                     DRRVGTSIEVAASRERALEAFLNHNDLAQWWGVAKSLIDPKPQGLWALAWEPGEKGYR
                     YVTAGVIGMFDPPERIIIDQLAYFNPEFPILAPMRLGLGFDGDDTRTQISVVQTGYGT
                     GPDWDRYYEAVVQGWPMALARLKEFLERKS"
     gene            2149..2469
                     /locus_tag="AC16GM_09105"
     CDS             2149..2469
                     /locus_tag="AC16GM_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR2923865.1"
                     /translation="MWSALRKARDILRQRIDFCVALYRDPRTPRSARVFLWLALGYAA
                     LPFDLIPDFIPVIGHIDDAVIIPVLLLIAFRLIPKEVYQEHHDHFFGKPARFVNPGGC
                     GGIQ"
     gene            2493..3476
                     /locus_tag="AC16GM_09110"
     CDS             2493..3476
                     /locus_tag="AC16GM_09110"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR2923866.1"
                     /translation="MKISMLETTAVGSYPKPDYLVKARNRFAKGKIPYTELHELELQA
                     TREWIKIQEDVGLDILVHGEQERGDMTTYFAENGRLEGLGISGLVRSYGNRYYRKPVV
                     QGEIKRPRPMTVEMFKYAQGLTKKPVKGMLTGAYTMCDWSFNTYYPTRRDVVLAFAKV
                     LHEEVADLEKAGAKYIQIDEPALSTRPDEVDLAIEAMKIVTAGIKAKTISHICYGDFR
                     VMYPRVLDLPVDVLDLEFANSRFQNLEIFRTPKFTKGVSVGVVDIHSHVIETKEEVKA
                     GIKKALEVFDVDKVWIDPDCGLKTRTVQEAIDKLKVMVEAVGEVKRELGLS"
     gene            complement(3509..4213)
                     /locus_tag="AC16GM_09115"
     CDS             complement(3509..4213)
                     /locus_tag="AC16GM_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923867.1"
                     /translation="MPSISKYNELLSKQASNIPKQKVSVLAQKGWVIMTFLKDSNWNI
                     IVKLLFAVGMITQPVLTVSAAPATKNSSQPLKQKTSSMVTEETKQLTEKMQAKYGDEY
                     SYFTYFRFCHAKMGIWTSSGLGSGIATEKGRRDSVILITVIANNDAVQRAAVRWAEKQ
                     GYSGMGKYDKASFRIDIIRGFFVEQTLKKIYALQASQSWSRSLWAVKIYFEEGSNSYY
                     SALIENGQISDVIEHN"
     gene            complement(4630..>5068)
                     /locus_tag="AC16GM_09120"
     CDS             complement(4630..>5068)
                     /locus_tag="AC16GM_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR2923868.1"
                     /translation="LTLEDFAQRHQTPATPNVLAIYLLGKVFERLVKKGIEKVERETI
                     QKAKMLYNWLNGHPSLRPFVPNPADRSLTVATIVLPKNWTSKSLQERLAPKNMAVGSG
                     YGKYKETQIRIANFPQHTEDDLKRVQKAVDQVLPVRPTVKTTR"
BASE COUNT         1135 a         1288 c         1407 g         1238 t
ORIGIN      
        1 tggacatcgt cacgaaggag ccgaaggaag ccatcgtcga gcgttccgac gtctgcgtgg
       61 ttccctgcta cggcgtcgtg gcggaggcgg tcgtcgcatt agtcttggcg aatgccttct
      121 tggaaaagtt cggcggcgac agtctggtgg aaatacgcga tcactttcaa acctacaacg
      181 aacggaaatt ctgatgctca aaacgaccgt cgtcggcaac tatcccaaga tttccagcga
      241 ccgctccgcg cccaatctgc gcaacgcgct gaatcaagcc gagagcggca aaatcactcc
      301 gcagcaggta gaagaggttt accagagtac catccggcgg gtcattgagg agcaaaagcg
      361 tgcgggggtc gatctcatta ccgacgggca aatccgctgg aacgacatcg tgacgccttt
      421 cgccaaaaaa atttccggct tcgagattaa cggcctcttg cgtttcttcg acaacaacgt
      481 ctactaccgc aagccggtgc tgaaagccaa accggcgttc aatggctccg cggcggtgga
      541 gcaattccgt tttgcacaga agtttgctgg cgaaaatctc aaggcggtca tccccgggcc
      601 gttcaccttt gcgcggatgt cggtggatga gtttttcggg aatgaagaga agttcgttct
      661 gaccctggct gaactcctgc accaggaagc ggtcgcgctg gtcgaggccg gagcgacctg
      721 ggttcagata gacgagccgt gcctctgcgt cgaacctggc aagattgatc tggttcgccg
      781 gggcatcgaa atcgtcaccc gcggtctgaa agccaaaaca atgctcttcc tctactttgg
      841 gagcatcaag ccattagtac cgaaactatt tgagttcccc gtcgatgcct ttggcgtcga
      901 ttgtgtctca cggcctgaga atttttcgct tttgcttaca gcgccgaagg ggaaagggat
      961 ctgctgtggc tgtctggacg ggcgcaatat caaactggag acggatcggg aactctttcc
     1021 ttatttcgag aaactggcgg cgcgtaccga cggcggtgaa gtattcattt cacccagcag
     1081 cggactcgaa tttctgccgc acgccgacgc ggtgcagaaa ttagagcgga tggtgaccgc
     1141 cgttcggacg ttcaacacac ggtgacgatg ctgaccgatc gcgtgatcaa aacctcaatc
     1201 accattaagg cgccggccga ggatattctc accgcgtttc tggatatcgg ctggatgcgg
     1261 gagtggtggg ggatagaagg cggcctggtt gagccgaagc gcgacggcct ctggacggtc
     1321 gtctgggggc attcggcgga aggctaccag gccattggca ccggcatcgt tcgccggctg
     1381 ggtccctacc ttctggaaat tgactcgaat ctggtgttct tcccacagcg gcagattctg
     1441 ggaccgaggc gcttccagct ccagtgccgg ccggaaggag ataagacctt cgtggacatc
     1501 gaagaatctg gattccagtc cggcggtgac tgggattggt tttacgaatc agtacaaaag
     1561 ggctggcaag aagtcttacc ggtgctgaag agttttttgg aaagacccca cgcccgagcg
     1621 accttgccat ccgccgctcc cgtcagcgac cgtcgagtcg gcacctctat cgaggttgcc
     1681 gccagccgcg agcgcgcttt ggaagcgttc ttgaaccaca atgacctcgc gcagtggtgg
     1741 ggagtggcga aaagtctcat cgaccccaaa ccgcagggtc tatgggctct ggcctgggag
     1801 ccgggcgaga agggctaccg ttacgtaact gccggtgtta tcgggatgtt cgatccgccg
     1861 gagcggatca tcatcgacca gctggcctac ttcaaccccg aatttccgat cttggcgccg
     1921 atgcgcctcg gcctcggttt tgacggggac gacacccgca cgcaaatttc ggtggtgcag
     1981 accgggtacg gcaccggccc ggactgggat cgctactacg aggcggtcgt gcaagggtgg
     2041 ccgatggccc tggcgcggtt aaaagaattt ttggaacgca agagttgatt ggactacagt
     2101 caaccagaac aataggccca ttttgtcagt gctcgaggaa gaggtatcat gtggagtgcg
     2161 ctacgtaagg cacgcgacat cctccgccag cggattgatt tctgcgtggc gttgtaccgg
     2221 gatccacgca ctccccgcag tgcgcgagtg ttcctctggc tggcgctggg gtatgccgcc
     2281 ttgccttttg acctcattcc cgattttatt ccggtgatcg gccatattga cgatgccgta
     2341 attatcccgg ttctgctttt gatcgccttc cggttgattc cgaaagaggt ctatcaggag
     2401 caccacgacc atttcttcgg aaaaccggcg cggtttgtaa atccaggggg gtgcggaggg
     2461 attcagtaag actgtggtgt aggtgaaaag aggtgaaaat ttcaatgctg gaaacgaccg
     2521 ccgtcggcag ttacccgaag cccgactatc tcgtcaaggc gcgcaaccgc tttgcgaagg
     2581 ggaaaattcc ctacacagaa ctgcatgaat tggaactcca agcaacccgg gaatggatca
     2641 aaatccagga ggatgttgga ctggacattc tggtgcatgg cgagcaggag cggggtgata
     2701 tgaccactta ctttgccgag aatggccgtt tagagggact gggtatctcc ggcttggtgc
     2761 gctcctacgg caaccgctac tatcgcaagc cggtggtgca gggagaaatc aagcgcccgc
     2821 gtccgatgac cgtcgaaatg ttcaaatacg cgcaggggct gaccaaaaag ccggtcaaag
     2881 ggatgctcac cggggcttat acgatgtgcg actggtcgtt caacacctac taccccacgc
     2941 ggcgggacgt ggtgctggcc tttgcgaaag tcttgcacga ggaagtggcc gatctcgaaa
     3001 aagcgggcgc caagtacatt cagattgacg agccggcgct ctcaacccgt cccgatgaag
     3061 ttgatctggc cattgaagca atgaaaatcg tcaccgcggg tatcaaagcc aagactattt
     3121 cgcacatctg ctacggcgac tttcgggtga tgtacccccg cgtgctcgat ttgccggtgg
     3181 atgtgctcga tttggagttc gccaactcgc gctttcagaa cttggagatc ttccgcacac
     3241 ccaagttcac caagggcgtg tcggtgggcg tggtggatat tcacagccac gtcattgaga
     3301 ccaaagaaga ggtgaaggcc gggatcaaaa aggcgctgga ggtttttgac gtggacaaag
     3361 tctggattga cccggactgc ggcctcaaaa cccgcactgt gcaggaggcg atcgacaaac
     3421 tcaaagtgat ggttgaggcg gtgggggagg taaagaggga actggggtta agttagtcct
     3481 ctacacccaa atttaccaca cttgcttttc aattgtgttc aatgacgtca cttatctgtc
     3541 cattctcaat tagagccgag tagtaagaat tcgacccttc ttcaaaatat atttttacag
     3601 cccaaagaga acgggaccag ctttgactcg cctgcaaagc gtatatcttt ttcagggtct
     3661 gctcgacaaa gaaaccgcgt atgatatcga ttctaaagga tgctttatca tactttccca
     3721 ttccagaata tccttgcttt tcggcccagc ggacagcggc ccgttgaact gcatcattat
     3781 tagcaattac agtaataaga ataacactat ctctgcgccc tttttctgtt gcaatgccgg
     3841 agccgagtcc tgaggatgtc caaattccca tcttggcgtg acagaatctg aagtaagtga
     3901 agtaactgta ttcgtcgccg tattttgctt gcatcttttc ggtcaattgt tttgtttctt
     3961 ccgtaaccat tgaagaggtc ttctgtttta atggttgtga agagttttta gttgcgggtg
     4021 cagctgaaac agttaagact ggttgcgtaa tcataccgac agcgaatagc agcttgacta
     4081 taatgttcca attactgtcc ttcaggaaag tcataataac ccatcctttc tgcgctaaca
     4141 ccgacacttt ttgctttgga atgttacttg cctgcttgga taagagttca ttgtatttgg
     4201 aaatagaagg cattatttct ccccgcagtt taaggccatc accctcttgt gtgatgttgg
     4261 tataataccc tctttaatct ttggtcgtct ttccctcatt tattaacagt atcgacagtc
     4321 tcccgtttac ctaaaccgta ttttgcctta gaacatctct ggttaatgct gaagcgtagc
     4381 agaggtcttc agacttccat tttccgtttt ctcctgtccc cctgtattta agggggttgc
     4441 ggggacgaac gtaggggcgc accttcaggt gcgccctggg tgaatctaaa ggttcaccct
     4501 acatttttta agggcgccga atagaaaacc attcgtcgcc accgaaggtc gggaagtccg
     4561 cggatcgacc ggtagatttt tcggtggcgc atattggttt ttgaaatgtg cgcccacagg
     4621 actattttgt tatcgagttg ttttgactgt gggacgtact ggtaaaactt gatcaactgc
     4681 tttctgaact cttttcagat catcttctgt atgttgcggg aaattagcaa ttcgaatctg
     4741 agtttcttta tactttccat aacccgaccc gacagccata tttttgggag cgagccgctc
     4801 ctgcagtgat ttcgacgtcc aattcttcgg cagcacgatg gtagcaaccg tgagcgagcg
     4861 gtcagccggg ttcggcacga acgggcggag cgagggatgc ccgttaagcc aattgtaaag
     4921 cattttggct ttttgtatcg tttcccgctc aactttttca attccctttt tgactaatcg
     4981 ctcaaagact tttccgagca ggtagatagc cagcacgttc ggggtggcgg gggtttggtg
     5041 cctttgggca aaatcttcca gcgtcaaa
//
LOCUS       DBNGZD010000341         1686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1027526, whole genome shotgun sequence.
ACCESSION   DBNGZD010000341 DBNGZD010000000
VERSION     DBNGZD010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1027526"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..806)
                     /locus_tag="AC16GM_09125"
     CDS             complement(141..806)
                     /locus_tag="AC16GM_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923869.1"
                     /translation="MKKEIPLAIMFITGVFMAVQFFIPHPTLAMIYQDFNSTVQVVGA
                     VALVLGIFSILSIHVTKIGKRQPGYGYSYFLIVFFFLMAASGFVPAGLQMGSFLGHGG
                     REEGSLFLNFYNYVLSPVQSSMFALLAFFIASAAFRAFRARSVIATLLLSTAIVVMLG
                     RVPIGPAMFGDWIADLANWIMAVPNAAAKRAIIVGVGLGILSLSLKMVLGIERSWLGG
                     REG"
     gene            complement(803..>1686)
                     /locus_tag="AC16GM_09130"
     CDS             complement(803..>1686)
                     /locus_tag="AC16GM_09130"
                     /inference="COORDINATES: protein motif:HMM:NF042826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR2923870.1"
                     /translation="RRPYVYKIKAQTAGGTAGESLVSSPVAAKPQWWNSTPDYNRIFV
                     GMVFVVGFILYNIRKAGKKELYIRPIAGINAIDEGIGRATEMGRPILFVCGLGTAADA
                     ATIAAFSVLGRVSQKVAQYQSKLIVPCYDPIVFTVCQDVVKGAYSNAGRPEAYNEKDV
                     FFVTQDQFPYVAAVNGIMLREKPATNFYLGQFYAESLILAETGFQAGSIQIAGTDQLI
                     QMAFFIVACDFTLIGEELYAASAYLSKEPEQVGSIKGQDWGKVVFTTLSLLGVISVFL
                     ESKIHLFVWFRKLFMAQ"
BASE COUNT          419 a          474 c          480 g          313 t
ORIGIN      
        1 caccgcgtcg tagagcgctt tcacttccgg ggtgacttta atcggcaggc cgatgtagaa
       61 gaagaagggg gtggtgacgg ccacggcgat ggccaaaaag atccagcgcc ggtcgatggt
      121 gagcaatttt tgaaaaatgc tcatccttcc cgtcctccca gccagctccg ctcaatcccc
      181 aggaccattt tcagcgaaag ggagagaatg cccaaaccca cgcccacaat gatcgcccgc
      241 ttggcggcgg cgttgggaac cgccataatc cagttggcca gatcggcaat ccagtcgccg
      301 aacatggccg ggccgatcgg cacccgtccc agcatgacga cgatggcggt cgaaagcaaa
      361 agggtggcga tgacgctgcg ggcccgaaag gcccggaagg cggcggaggc gatgaagaag
      421 gccagaagcg cgaacatgct cgattgcacc ggcgacagga cgtagttgta gaagttgaga
      481 aagagcgacc cttcttcccg gccgccatgc cccagaaaac tccccatctg gagaccggcc
      541 ggcacaaagc cggaagccgc catcaggaaa aagaagacga tgaggaaata actgtagccg
      601 taccccggct ggcgcttgcc gattttagta acgtgaatgg agagaatgga aaaaatcccc
      661 agcaccagcg ccaccgcacc caccacctgg acggtcgaat tgaagtcctg gtaaatcatg
      721 gccagggtag gatggggaat gaaaaactgg acggccataa agacgccggt aatgaacata
      781 attgctaaag gtatctcttt tttcattgcg ccatgaatag ttttctgaac cagacaaaca
      841 agtgaatttt gctttccagg aaaaccgaaa tgacccctaa gagggaaagg gtcgtgaaca
      901 ccaccttgcc ccagtcctgc cccttgatgg agccaacctg ctccggctcc ttggaaaggt
      961 aggccgaggc ggcgtacagt tcttcaccaa tgagggtgaa gtcacaggcc acgatgaaga
     1021 acgccatttg aatgagctgg tcggtgccgg caatttgaat cgacccggcc tggaagccgg
     1081 tttcggccag aatcaacgac tccgcgtaaa actgtcccag gtagaagttg gtggccggct
     1141 tttcccgcag catgatcccg ttgaccgcgg cgacgtacgg gaattgatcc tgggtcacga
     1201 aaaagacgtc tttctcgttg tacgcttccg gccgcccggc attcgagtag gcacccttga
     1261 ccacgtcctg gcacacggtg aagacaatcg ggtcatagca ggggacgatc agcttgctct
     1321 gatactgggc taccttttga gagacccgcc ccaggacgga aaaagcggca atggtagcgg
     1381 cgtccgccgc cgtccccaaa ccgcagacga agagaatcgg ccgtcccatt tcggtcgccc
     1441 ggccgatgcc ttcatcgatc gcattaatac cggcaattgg tcggatgtag agctcttttt
     1501 tcccggcctt acggatattg tagagaataa agccgaccac aaagaccatc ccgacaaaaa
     1561 tccggttgta atccggtgtg ctgttccacc actgtggttt tgctgcaacc ggggatgaga
     1621 cgagcgattc accggccgta ccccccgcgg tctgggcttt gatcttgtac acatagggac
     1681 gacgat
//
LOCUS       DBNGZD010000342         3660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_888296, whole genome shotgun sequence.
ACCESSION   DBNGZD010000342 DBNGZD010000000
VERSION     DBNGZD010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_888296"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="AC16GM_09135"
     CDS             complement(<1..398)
                     /locus_tag="AC16GM_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase/protein-tyrosine-phosphatase
                     family protein"
                     /protein_id="HIR2923871.1"
                     /translation="MFTVLFVCTGNTCRSPMARGIFRQLLTEQGAGDIELLSAGMAAV
                     EGLPATELATATARRWRIDISSHQSRRLDPELVARADLILAMSDEHVEAILALDPEAA
                     ERTFLLKAFPLPDRFDPLHAIADPIGKGPE"
     gene            complement(404..1189)
                     /locus_tag="AC16GM_09140"
     CDS             complement(404..1189)
                     /locus_tag="AC16GM_09140"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR2923872.1"
                     /translation="MSLRPLRVVNPHRPSAFSIIRRPPLATPTLPLADCIQPSPPLSQ
                     ADRPRTTRWLRVRGGRVTAAQLRIAVDTLAQGGIVVLPTDTVYGLAADALNRKAVSRL
                     AQVKGRTAKQPVAILLGSAKEMKTWGKIFPTVKRLSETFLPGPFTLVVPTTFQTPRHL
                     VQNGQIGLRVPKSRLAQKLVVCLGRPLAATSANRMGQPPCRTGREAYRVFRGAVDLVL
                     DAGVKSGPASTVVQVVKGKLRVIREGPVSIRTLQQAGFRVGQP"
     gene            complement(1199..2278)
                     /locus_tag="AC16GM_09145"
     CDS             complement(1199..2278)
                     /locus_tag="AC16GM_09145"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR2923873.1"
                     /translation="MLYAIIIAGGRGERFWPLSRSGHPKQLLRLLSEKTLLQETIDRV
                     QCFIPIDRTVVVTGENMQKAVLGAVPYLREKHILAEPEGRNTCLAIGLAAVHLVKRDP
                     DAVMVVLSSDHAVQPAERLVEILKTAAQVASSGPYLLTLGVVPTRPETGYGYIELGER
                     HAQQDGIEVFRVGQFKEKPSRTVAQEYYFDRKHLWNSGMFVWTARTFLDAVRQHLPEL
                     AGLLEAYAPAIGTPKEKQARLVLYQSAESISVDCAILEKAKNVLTIKSDFKWDDVGSW
                     LALERVKPKDRENNVILGDSLLVDSYETTLVNDGEGLVVTYGVSDLVIVKTNQIVFVA
                     HKTKVGDLKQLVAKLNQDRKAQAYL"
     gene            complement(2366..3388)
                     /locus_tag="AC16GM_09150"
     CDS             complement(2366..3388)
                     /locus_tag="AC16GM_09150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR2923874.1"
                     /translation="MTKRLRWQFLIGIGISVVFLYLAFRGVKLSELKRALAAVNYWYL
                     LPSIALILASFVVRAYRWGWMVAPIKPARFGNLFAATLIGFMANNVLPFRLGEIGRAY
                     SLGHLEKISRSSSLATIILERFFDTVGLLLLTIVILIWFPFPPHLRWIPPMAVTVTAV
                     ALLFLVFLKLKGERAGEILAHLLRVLPGQFGQKTGEIVTKFSQGLGLLSRPAAVLLVL
                     FHSLVIYCILAAAGYVVLLAFGLNPGILAPFVLLVVVSLGLILPAAPGFVGTFQQLTI
                     WALAPFGINKSTALSFSIVLHASHYFPVTLLGLYYLRKKHLSLAAVEKLASGPDPASL
                     LPGKEN"
     gene            complement(3385..>3660)
                     /locus_tag="AC16GM_09155"
     CDS             complement(3385..>3660)
                     /locus_tag="AC16GM_09155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2923875.1"
                     /translation="TNIEANACGTPAVAADVPGLRDSVSDGTSGLLYPYGDVAQLAER
                     LIRILTDVRLRGNLERGALAWARKFQWDTAARELAGVIDEVVGRERR"
BASE COUNT          762 a         1103 c         1094 g          701 t
ORIGIN      
        1 tcttccggcc ccttgccgat gggatcggca atggcgtgca gcggatcgaa tcgatcgggc
       61 aggggaaatg ctttgaggag aaaggtgcgc tccgcggctt ccggatccag agccaggatg
      121 gcttccacgt gctcgtccga catcgccaga atcaagtcgg cccgggccac cagctccggg
      181 tccaaccgcc gcgactggtg gctgctgatg tcgatcctcc accggcgggc ggtggccgtg
      241 gccaattcgg tagcgggcaa cccttcgacg gcggccatcc cggccgaaag aagctcaata
      301 tctccggccc cctgttcggt caataactgg cggaagatcc cccgggccat tgggctgcgg
      361 caggtgttgc cggtgcagac aaagaggacc gtaaacatgc cggctacggt tgcccgaccc
      421 gaaacccggc ctgctgcaag gtgcgaatcg agacaggtcc ttcgcgaatc acccgcagtt
      481 tccccttgac aacctgcacc acggtggatg ccggccctga ttttactccc gcatcaagta
      541 cgagatcgac tgcgccgcga aacacccggt atgcttcccg gccggttctg cagggcggct
      601 ggcccatacg attggcgctc gtagccgcca ggggtcggcc caaacacacc acgagtttct
      661 gagctaaacg cgatttgggc actctcaggc cgatctgacc gttttgaacc agatgtctgg
      721 gtgtctgaaa agtggtggga acgacaaggg tgaaaggccc ggggagaaat gtttcgctca
      781 gccgctttac tgtgggaaat atttttcccc atgtcttcat ttccttcgcc gaccccaaaa
      841 gaatcgccac gggctgtttg gctgtccttc ccttaacctg cgccaggcgc gacaccgcct
      901 ttcgattcag cgcgtcggcc gccagcccgt aaacggtatc ggtcggcaga acgacgatgc
      961 ctccttgcgc cagcgtgtcc acggcaatgc gcaattgtgc agcagtgacc cgcccgccgc
     1021 ggacgcggag ccagcgggtc gttcggggcc ggtcggcttg ggaaagcggc gggcttggtt
     1081 gaatgcagtc ggccaggggt aaggtgggcg tagccaaggg gggtcgccgg atgatggaaa
     1141 aagcagacgg gcggtgcgga ttgaccacgc gcaatggtcg cagactcaag tctcccgctt
     1201 acaggtaggc ttgcgccttc cggtcttggt tcagtttggc cacaagttgc ttcagatcgc
     1261 ccactttggt tttatgcgcc acgaagacga tctggttggt tttgacaatc acgagatcgg
     1321 acacgccgta ggtgaccacc agcccctccc cgtcattgac cagcgtggtt tcgtacgagt
     1381 cgaccagcag cgaatcgccc aaaatgacgt tgttttcgcg gtctttcggc ttgacgcgct
     1441 ccaaggccag ccaagacccg acgtcgtccc atttgaaatc cgactttatg gtcagaacgt
     1501 ttttggcttt ctccagaatg gcgcagtcga ccgaaatgct ctccgccgat tggtacaaga
     1561 ccagccgagc ctgtttttcc ttcggcgtgc cgatggccgg tgcatacgct tccagcagcc
     1621 cggcgagctc cggcaggtgc tggcggacag cgtccagaaa ggtcctggcg gtccagacga
     1681 acatcccgga attccagagg tgtttgcggt cgaagtagta ctcctgggcc accgtgcgcg
     1741 agggtttctc tttgaactgg ccgacccgga aaacctcaat gccatcttgc tgcgcatgcc
     1801 gctcacctaa ctcgatatag ccgtaaccgg tctcgggccg ggtgggcacg acccccaggg
     1861 tgagcaggta gggcccggag gaggccacct gtgctgcggt tttgagaatt tccaccagtc
     1921 gctcggccgg ctgaacggca tggtccgagg agagcaccac catcactgcg tccgggtcac
     1981 gtttgaccaa gtgcaccgcc gccagcccaa tggccaaaca ggtgttgcgt ccttccggct
     2041 ccgccagaat atgtttttcc ctcaagtagg gcaccgcccc cagaactgct ttttgcatat
     2101 tttcgccggt gacgaccacc gtccggtcta tcggaataaa acattgcacc cggtcgatgg
     2161 tctcctgcag gagcgttttc tcggagagca aacgcaagag ctgcttgggg tggcctgagc
     2221 gcgagagcgg ccagaagcgt tcccctcgtc ccccggctat tataatggcg tagagcatgg
     2281 tcacctgctc cttttcacac cacaaactta agaggcaatc cgaagcaagt caagaacttg
     2341 gattttgcct gttcgttggt gctctttaat tttcttttcc ggggaggagg ctggccgggt
     2401 cgggcccgct ggccagcttt tccactgccg cgagcgaaag gtgttttttc cgcaggtagt
     2461 acagacccaa aagcgtcact gggaagtagt ggctggcgtg taaaacgatg gaaaacgaca
     2521 gcgcagtgct cttgttgatg ccgaacggcg ccagagccca gatggtgagc tgctggaacg
     2581 tgccgacgaa accgggcgcc gccgggagaa tcaaacccag ggagacgacc accaaaagca
     2641 caaagggcgc caggattccc gggttcagcc caaaggccag aaggacgacg tatccggcag
     2701 ccgccaggat gcagtaaatc accagggaat ggaacaggac caaaagcacc gccgccggac
     2761 gagacaggag ccccagcccc tgggaaaact tcgtgacgat ttcgccggtt ttctggccga
     2821 attgcccggg caaaacccgg agcaggtgcg ccagaatttc tccggcacgt tcacctttga
     2881 gtttgaggaa aaccaaaaac agaagcgcca cggcggtgac cgtgaccgcc atcggcggaa
     2941 tccagcgcag gtgcggggga aatggaaacc agatgaggat gacaatcgtc aagagcaaaa
     3001 gcccgaccgt gtcgaagaaa cgttccagaa tgatggtggc taaagaggaa ctgcggctga
     3061 ttttctccag gtggcccagc gagtaagcac ggccgatttc ccccaaccgg aagggcagga
     3121 cattgttggc cataaagccg atcagcgtgg cggcgaagag gtttccaaag cgcgctggtt
     3181 tgatgggtgc gaccatccac ccccagcggt acgcccgtac gacaaagctg gccaaaatca
     3241 gggcaatgct tggcaacagg taccagtagt tgacggcggc taaggcccgt ttcaattccg
     3301 aaagtttcac cccgcgaaac gcgaggtaga ggaatacgac gctgattccg atcccgatga
     3361 gaaactgcca gcgcagccgt ttcgtcatcg cctttccctc cccactactt catcgatcac
     3421 tccggctaat tcacgcgccg cggtgtccca ctgaaacttc cgggcccacg ccagtgcgcc
     3481 gcgttccaga ttaccccgca ggcgcacgtc ggtgagaatc cggattaacc gctccgccag
     3541 ttgcgccacg tctccgtacg gatacaaaag tccgctggtc ccgtcactga ctgagtcgcg
     3601 caagcccggc acgtcggccg cgaccgccgg tgtgccgcag gcgttcgctt cgatattggt
//
LOCUS       DBNGZD010000343         2519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_958183, whole genome shotgun sequence.
ACCESSION   DBNGZD010000343 DBNGZD010000000
VERSION     DBNGZD010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_958183"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="AC16GM_09160"
     CDS             <1..748
                     /locus_tag="AC16GM_09160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2923876.1"
                     /translation="GRGQKKVVRGAAAVGADSGEMAQRLGESTSEATQIFTIPNGFDP
                     ADLPKNVRKKGAPFTIAYWGTVTPKRSFDRFFAALALWRERARLSSERFRFLQIGAAL
                     DGDPFAGARRLGLTEAVEAKGYLPHRQGLAELAQADLFLLLIGEGEGSLGVLTGKLFE
                     YLALGKPILALVPPNGEAARLIENLGAGMVVAPHNQAGMVAALEHFHALFLKKKMSSG
                     VAAERLAPFSRLEQTRTLAGLFDRIVAKRT"
     gene            745..2010
                     /locus_tag="AC16GM_09165"
     CDS             745..2010
                     /locus_tag="AC16GM_09165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR2923877.1"
                     /translation="MNRERTALIGILAVGLGLRLLYIFAFPLYQAADEYPHFHYIQHL
                     GLTHALPRSTPQYPEYEGFQPPLYYFLSSLVYAPLARYDRDRESSAPLALILPESERA
                     KPPNSLALFFRVLSTLLAVVTLYVVYLISRNFFPDSAGLRLASVAWVAVLPAYATTTA
                     AITNDALAVLVGALLILFLVRQSGWDSKIAFGFGILTGLGALTKHNLLIFLPAGIVAP
                     FFFQSDKRGKAMLTFGLGFVATTFFWYWHNWQTYGSLLAINPDFPTASLFDVRRAQVL
                     QAVRNLARSFWLAGGINYKVMLPVWVYVVVFGGLSLLALSGWVQCARHQWPEFRQGLK
                     QYRIYLLLAILLIFASLFYSLSYPPGRMTSWGKNLFPLLPLWSFFFVGGWKYFWPRAG
                     LGLVWSCVFLLVIVNLVYLFGCVIPYYST"
     gene            2007..>2519
                     /locus_tag="AC16GM_09170"
     CDS             2007..>2519
                     /locus_tag="AC16GM_09170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2923878.1"
                     /translation="MNAPASVSVIIVTWNCAGDIARCLQSLSVEQGLIGEVALIDNAS
                     TDGTVKLVEQQFPQVRVIRHRENLGYAAAVNQGLREVSGELVLLLNPDAWVKEAGLRP
                     LLDYLHKHSDVGACAPRLLFPDGRFQASCRRFPTVGRLLIEASGLGLILPFLSHWRWK
                     LSEKEHQEGPV"
BASE COUNT          431 a          716 c          764 g          608 t
ORIGIN      
        1 cggccggggg cagaagaaag tcgtccgcgg cgccgctgcg gtgggggcgg actcaggcga
       61 aatggcccag cggctgggcg aaagtacgag cgaggctact caaatcttca ccattccgaa
      121 cggattcgat ccggcggatc tgccgaaaaa cgtccggaaa aaaggagccc cctttaccat
      181 tgcatactgg ggaacggtga cccccaaacg atctttcgat cgattcttcg ccgccttggc
      241 tctgtggcga gagcgtgccc gcctgagttc ggagagattt cggttcctgc aaatcggcgc
      301 ggctctggac ggcgacccgt tcgccggagc taggcgtttg ggattgactg aggcggtgga
      361 agcgaagggg tacctgcccc accggcaagg gctggcggaa ctggcgcagg cagacctctt
      421 tttgcttttg atcggtgagg gagaagggtc cttgggggtt ttaacgggga agctctttga
      481 atacctggct ctgggcaaac cgattctggc tctggtgccg ccgaatggtg aagcagcccg
      541 gctcattgaa aacctcggcg ccgggatggt ggtggcgcca cacaaccagg cagggatggt
      601 ggctgccctg gagcatttcc acgctctttt tttgaaaaag aaaatgtcga gcggcgtagc
      661 cgccgaaagg ctggccccct tttcccggct tgagcagacc cggactttgg cggggctttt
      721 tgaccgcatc gtggcaaagc gcacatgaat agggaacgga ccgcactgat aggcatcctg
      781 gcggtgggac tcggtttacg attgctctac atttttgcct ttcccctcta ccaggcggcg
      841 gatgaatatc cgcatttcca ctacattcag catctcgggc tcacccatgc gttgccgcgc
      901 tcaacgcctc agtatccgga gtacgagggg tttcagccgc cgctctacta cttcctctca
      961 tctctggttt atgcgcccct agcgcggtat gaccgtgacc gggagagcag cgcccccctt
     1021 gcgctcattc tgccggagag cgagcgcgcc aagccaccga acagcttggc actcttcttc
     1081 cgagtcctct cgactttgct ggccgtagtc actttgtacg tggtgtacct catcagcaga
     1141 aactttttcc ccgacagcgc cgggttgcgg ctggcgagtg tggcctgggt tgcggtttta
     1201 cccgcctatg cgaccaccac cgccgccatc accaacgacg ctttggcggt gctcgtggga
     1261 gcgctgctca tcctttttct ggtgcggcag agcgggtggg attccaaaat cgctttcggc
     1321 ttcgggattc tcaccgggct gggagcgctc accaagcaca accttttgat ttttcttccg
     1381 gcggggatag ttgctccatt tttctttcag tcggacaagc gcgggaaggc aatgctcacg
     1441 ttcggtctgg gttttgtcgc cacgacattt ttctggtact ggcacaactg gcagacctac
     1501 ggttcgcttt tggcgatcaa ccccgatttt cctaccgcca gtctgttcga tgtccggaga
     1561 gcgcaggtgc tccaggcggt gcgcaatttg gcccgcagtt tctggctggc gggggggatc
     1621 aactataagg tcatgctccc ggtgtgggtt tacgtggtgg ttttcggcgg gctctcgctt
     1681 ctggcactct cgggttgggt gcagtgtgcc cgacaccagt ggccggagtt ccggcaaggc
     1741 ctaaaacagt accgcatcta cctgctccta gcaattcttt taatttttgc atcgcttttc
     1801 tattcccttt cctacccgcc ggggcggatg acctcctggg ggaaaaatct cttcccgctt
     1861 ttgccgctct ggagtttttt ctttgtggga gggtggaagt atttctggcc ccgggcgggt
     1921 ctcgggctgg tctggtcgtg cgttttcctg ttagtaatcg ttaatctcgt ctatttgttc
     1981 ggctgtgtca tcccctacta ttcgacgtga acgcaccggc ttcggtttcg gtcattatcg
     2041 tcacctggaa ctgcgcgggg gatatcgccc gttgcctcca gtcgctgtca gttgaacagg
     2101 gtttgatcgg cgaagtagcc ctcattgaca atgcctcgac tgacgggacg gtaaaactgg
     2161 tggagcaaca gttcccgcag gtgcgcgtca tccgccaccg cgagaatctg ggatacgccg
     2221 cagcggtcaa tcaaggtctg cgggaagttt cgggtgaact ggtacttttg ctcaacccgg
     2281 atgcgtgggt gaaagaagca gggttgcgcc cactcctcga ttacttacat aagcattcgg
     2341 atgtgggcgc ctgtgcgccc cggttgcttt tccccgacgg acggttccag gcctcctgcc
     2401 gccgctttcc cacggtcggg cgactgctga ttgaggcgag tgggctgggg ctcattctgc
     2461 cttttctctc acactggcgc tggaaactct ccgaaaaaga gcatcaggag gggccggtg
//
LOCUS       DBNGZD010000344         2315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_611341, whole genome shotgun sequence.
ACCESSION   DBNGZD010000344 DBNGZD010000000
VERSION     DBNGZD010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_611341"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="ispE"
                     /locus_tag="AC16GM_09175"
     CDS             <1..906
                     /gene="ispE"
                     /locus_tag="AC16GM_09175"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR2923879.1"
                     /translation="LSLSILGQRADGYHDLASLVQAIDLHDTLEFSPRPSGILVQTDQ
                     PELQGSDDNLIYQALQKLSQESRITSGVQVKLTKRIPLGSGLGGGSSNAATALWGGAQ
                     LWDLAKPVTELSRLGAEIGSDVPFFFSSGQAVIRGRGEVVEPLRLFADYRVVVVYPGF
                     GIGTQWAYQQLDFVRQERVPPGLTPVPGDGYLSNLETKEGFVAALSGFANDFEPTVAR
                     TYRVIGQIKQALVDSGAVFSSLTGSGSAVFGIFLDQPIEQEITRRLQALPEYRKPNLW
                     RVSVTRPVIAAEPVCASPDASTGSG"
     gene            1015..1341
                     /gene="spoVG"
                     /locus_tag="AC16GM_09180"
     CDS             1015..1341
                     /gene="spoVG"
                     /locus_tag="AC16GM_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010297759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HIR2923880.1"
                     /translation="MEITDIRITLRDEEKLKAIVNVTFDNCFAVRGVKVISGESGYFI
                     SMPSRKRPDGTFQDIAHPTNSEMRKKMEDKILEAYERALKSREAQAAAAPAPRVESAS
                     GGAASE"
     gene            1390..1461
                     /locus_tag="AC16GM_09185"
     tRNA            1390..1461
                     /locus_tag="AC16GM_09185"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1423..1425,aa:Gln,seq:ttg)
     gene            1675..2244
                     /locus_tag="AC16GM_09190"
     CDS             1675..2244
                     /locus_tag="AC16GM_09190"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR2923881.1"
                     /translation="MKRTSLNSIKVITMLLLGSALVALAGCSRGKEQQLQADLRKFTD
                     EQAMTATYLVRFDSLDFDVFSNQKWDLLNVSHADDITVNWPDGHQTKGIAEHIEDLKA
                     MFVFAPDTRIEDHPIKFGSGEWTCVTGTFAGTFTRPMPVGNGKTIPPTGKKFSLPMCT
                     VGHWKDGKMIEESLFWDNQTFMKQIGLAK"
BASE COUNT          520 a          628 c          681 g          486 t
ORIGIN      
        1 ctctccctct ccattctggg ccagcgcgcc gacggctacc acgacctcgc ctcgctggtg
       61 caggcgattg acctccatga cacgctggaa ttttcccctc ggccttcggg tattcttgtt
      121 caaacggatc agccggagct gcaggggtcc gatgacaatt tgatttacca ggcattgcaa
      181 aagctctctc aggaatcgag aataacgtcg ggagttcagg ttaaactgac caagcggatt
      241 ccactgggtt ccgggctggg cggcgggagt tccaatgcgg ctacggcgct ctggggtggg
      301 gcgcaactct gggatctcgc taagccggtc accgaattgt cacgcttggg agcggaaatt
      361 ggctcagacg tacctttttt cttcagttcc ggccaggcgg tcattcgcgg gcggggtgaa
      421 gtggttgagc ccttgcgact ttttgcggat taccgggtag tcgtcgttta cccggggttt
      481 gggattggca cccagtgggc atatcagcag ctggattttg tccgccagga gcgggttccc
      541 cccggcttga cacctgtacc cggcgacggg tatctttcca atttggagac aaaagaaggc
      601 tttgtggctg cgctttccgg gtttgccaac gacttcgaac caacggtggc tcgtacctat
      661 cgcgttatcg gtcaaatcaa gcaggcactg gttgattccg gcgcagtgtt ttccagcctc
      721 accggaagcg gctcggcagt gttcggcatt tttctggatc agccgatcga acaggaaatt
      781 acccgccgct tgcaggcgtt gccggaatac cggaaaccca acctgtggcg ggtgagcgtc
      841 acccggccgg tgatagcggc ggagccggtt tgtgccagtc cagacgcgag caccggttca
      901 ggatagacag ccccgcctct ggcggggcct tacggcaggg agcatatcga gagaggaatt
      961 gaggagtaat ggttcgatcc gcctcaggcg gactcaccat agaaggagca gtctatggaa
     1021 atcaccgaca tccggatcac cctgcgcgat gaggagaagt tgaaagccat cgtcaacgtc
     1081 accttcgaca actgctttgc ggtgcggggg gtcaaggtga tcagcgggga gagcggctac
     1141 ttcatctcaa tgccttcccg caaacggccc gacgggacct tccaggacat tgcgcacccg
     1201 accaactccg aaatgcgtaa gaaaatggag gataaaatcc tggaggccta cgagcgggcg
     1261 ctgaaatcca gagaagccca ggccgccgcg gctcctgctc cgagagtcga gtctgcttcc
     1321 ggcggggccg catcggagta gccattctct ccggcagccc cgctcttccg gtcaaagcga
     1381 tattcatttt ggggcgtagc caagttggta aggcacaaga ctttggatct tgcatcccag
     1441 gttcgaatcc tggcgcccca gataaaacaa ggatcaggga ttagagatta gggattagta
     1501 aattctcggc gtcagacgat atttccggtt cgcctgcccg agccgggtgt gggggctcgg
     1561 ccagagaatg cggtgccaca gcggttgccc ttgtgccgat tgcaagggag tctttttgac
     1621 taacgccgaa gcgaaagagg acgtaaatca tccaacaagc cataggaggt cgatatgaaa
     1681 aggacatcgc tcaactcgat caaggtgatc accatgcttt tgctgggatc ggccctggtt
     1741 gctttagccg gctgctccag aggcaaagag cagcaactgc aggcggattt gagaaaattc
     1801 actgacgaac aggcaatgac cgccacctac ctcgtccgct tcgactcgct ggattttgac
     1861 gtcttcagca atcaaaaatg ggacctgctg aatgtcagcc atgcggatga catcaccgtc
     1921 aactggcccg acgggcacca aaccaaaggg atcgccgagc acatcgaaga cctgaaggcg
     1981 atgttcgtgt ttgcgcccga taccagaatc gaagaccatc cgatcaaatt cggttccggt
     2041 gaatggacct gcgtcacggg cacattcgcc ggaacgttta ccagaccgat gcctgtcggc
     2101 aacggcaaaa cgattccacc caccggcaag aaattcagtc tgccgatgtg caccgtaggc
     2161 cattggaaag acggcaagat gatcgaggaa tctttgttct gggacaacca gacgttcatg
     2221 aagcaaatcg ggctggccaa atagacccac cggtcgttgc cggtttggga ttgcggttct
     2281 aaggttcaga aaatgaggaa tttcttgaaa aaact
//
LOCUS       DBNGZD010000345         2125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1861149, whole genome shotgun sequence.
ACCESSION   DBNGZD010000345 DBNGZD010000000
VERSION     DBNGZD010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1861149"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16GM_09195"
     CDS             complement(<1..206)
                     /locus_tag="AC16GM_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2923882.1"
                     /translation="MSTIGDHIRKRRLDLGLWQRQVAQQIGVDESTITNWEKHRNFPS
                     LRFMPRIIQFLGYDPCPAEADQPLP"
     gene            266..760
                     /locus_tag="AC16GM_09200"
                     /pseudo
     CDS             266..760
                     /locus_tag="AC16GM_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251974.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
     gene            1132..2040
                     /locus_tag="AC16GM_09205"
     CDS             1132..2040
                     /locus_tag="AC16GM_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR2923883.1"
                     /translation="MFCFEGCGKNERQIGQAGKKDQQQVSITIGVSLLTRTHPFYQDL
                     EAGLQAAAAAAGYKLLITAGEFDVAKQKDEIQDFIVRKVNAIIVSPCDSRSIGTAIKA
                     ANEAGIPVFTADIACLAAGVKVVSHVASDNVAGGRLAAQAVAAAIGGSGKVAIIDHPE
                     VESVIQRVRGFEEEMVKFDRIKIVAKLSGHGVKDQAFRAAEDLLQAHPDLKAIFGIND
                     DSALGALAAVEKSGKTGKVKIVGFDAVPEARRAIKDGKIYADVIQRPREIGEKTIEAV
                     KTYLSGGSLPPTVLTPCALLTQADAR"
BASE COUNT          488 a          550 c          629 g          458 t
ORIGIN      
        1 ggtaggggct ggtcggcctc ggctggacag ggatcatagc ccaggaactg gataatccgg
       61 ggcatgaacc gtagggaggg aaagtttcgg tgcttctccc aattagttat ggtgctctca
      121 tccacgccga tctgctgggc cacctgccgt tgccagaggc ccaggtcaag tcgtctcttc
      181 cggatgtggt ctcctatggt ggacaacgac ttagggtatg cttttgggag tggttttgga
      241 gcgcaaagtt ttgcgtaaca aaatggcaat gaacccctgt aactgtggct tctttggcga
      301 ccccggtcac gagtgatcct gcacgccgac gcagattcag aagtacatgt cgagaatttc
      361 tgggccgctc ttagaccgga ttgacattca catcgaagtg cccgcggtga aattccgtga
      421 attagccggt cagccgacgg gtgagagttc ggccgctatc cgtgagcgag tgaaccgggc
      481 gcggcaggtg caactggagc gtttcaagag cgaaaagtcg atttactgca acgcacacat
      541 gcagtcgaaa gaaatccgga agtactgcct cattgacgac aaatcgcagg aactcctgcg
      601 gctggcgatg caaaagttgg gtctctccgc ccgggcgtat gaccgcattc tgaaagtgtc
      661 gcggaccatc gccgaccttg aggggagtgt cgaaattcag ccgcaccacg tgtcggaagc
      721 ggtgcaatac agatcgctgg accgaaacct ctggctatag ttgaaagtct gtgcctattt
      781 ctacagtgta ctaagcttca gtgaaaatcc ggtgtcgggg aatttggcga ggtttcgcta
      841 taagtagttc gtttgtaaag aattcaaaat tagaccttag cgtgcagttc gggcggcgga
      901 acaactgaaa aagcagaaca ttgtcaccag gtttaccctc ccagggcaaa tattcctggt
      961 gcagcagaaa ctttaaaggc ttgcaccggc agaaggtgaa gtttatcttg gtcgggaata
     1021 tggagacgac cgcactgaag tttttctcgg aatgacaact ttcgacatta ttacgggtga
     1081 cccatgacgt tttgcacgaa ggtcctcata tcactccttg ccgcgagcct tatgttttgt
     1141 tttgagggat gcggtaagaa cgaacgacag atcggccaag ccgggaaaaa ggaccagcag
     1201 caggtgagca ttaccatcgg tgtctccctg ttgacccgta cgcacccatt ctatcaggat
     1261 cttgaggccg gcctgcaggc ggcggcggct gctgcgggtt ataagctcct gattacggca
     1321 ggcgagtttg atgtcgccaa acagaaagac gagattcagg atttcatcgt gcgcaaggtc
     1381 aacgccatta tcgtctcgcc ctgcgattct cgatcgatcg ggaccgccat caaagccgcc
     1441 aatgaggcgg gcataccggt attcactgcc gacatcgcct gtctcgcggc gggggtcaaa
     1501 gtcgtctccc atgttgcgtc cgacaacgtg gccggaggtc ggctggccgc tcaggcggtc
     1561 gctgcggcga ttggcggcag cggcaaggta gcgatcattg accacccgga agtcgaatcg
     1621 gtgatccagc gggtgcgcgg gtttgaggaa gaaatggtca aattcgatcg catcaaaatt
     1681 gtcgccaagc tttccggtca cggcgtcaag gaccaggcct ttcgtgcagc cgaagatctg
     1741 ctccaggccc acccagacct caaggcgatc ttcggcatta atgatgactc ggcattggga
     1801 gccttggcgg ccgtggaaaa atccgggaaa acaggtaaag tgaaaatcgt cggctttgat
     1861 gcagtcccgg aggcccgtag agctatcaaa gacggcaaga tctatgcgga cgtgatccag
     1921 aggccacgag aaatcgggga gaagaccatt gaggcggtca agacgtacct ttctggaggt
     1981 agtctgccgc cgacagtcct gactccctgc gccctcctca cccaggccga tgcccggtag
     2041 cggagccccg cggcgaattc ggacaacccg ctttttccca tgaccggagc tgcttcaccg
     2101 ttcttgcgga tgaagggcat cgaaa
//
LOCUS       DBNGZD010000346         3887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_958307, whole genome shotgun sequence.
ACCESSION   DBNGZD010000346 DBNGZD010000000
VERSION     DBNGZD010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_958307"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1165)
                     /locus_tag="AC16GM_09210"
     CDS             complement(290..1165)
                     /locus_tag="AC16GM_09210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR2923884.1"
                     /translation="MLKKRISVLIVPYPKGKALRLSIPLGVILLPAFFLFLFVISDVF
                     FSVNYIKKIYDNQKIAELSKQNAHMESKFFDAQALAHQLKTDILLILEHEKKVERFLG
                     LAERGRTPRSAELPELKLPPQPLPAEADLDLLLATTLQQREALNQIYDKFVVRRDRLD
                     HTPSILPVPGRLTRGFGLHSDPFTGQSEHHAGVDLAAPWGTPVQASATGRIVYAGWRS
                     GLGKAIIINHGRGFETYYGHLSAIRVTPGQRVTKGQLIGAVGSTGYSTGPHLHYEVHL
                     RTMPQNPMRYVLIER"
     gene            complement(1342..1482)
                     /locus_tag="AC16GM_09215"
     CDS             complement(1342..1482)
                     /locus_tag="AC16GM_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923885.1"
                     /translation="MNRRRKRRFKIPEKTAWHLYSLQNLNAEKFIRVVNKILRREFSL
                     IK"
     gene            2079..2663
                     /locus_tag="AC16GM_09220"
     CDS             2079..2663
                     /locus_tag="AC16GM_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR2923886.1"
                     /translation="MQARETAESDQALVVQALAGEKKAFAALLERHRQAVFHITYQIA
                     HNQEEARDLVQETFMKAFAGLATYRSQFRFTTWLYRIAANCAIDALRKKKIDALSLDQ
                     PLETGEGTVAFEVADLAADPEAELVAEQRRISIGEAIDALPKKYREVLVFRHREEKSY
                     EEISDILKIPVGTVKARIFRARELLKKKLKHYRP"
     gene            2688..3788
                     /locus_tag="AC16GM_09225"
     CDS             2688..3788
                     /locus_tag="AC16GM_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR2923887.1"
                     /translation="MNFRDKLTQRNVFRVATGAIAATALLLFSFAESQSRQYTYEFHK
                     ELPVSPGFVFSLTDPIGDIVVEGYAGRSIEIAARKVVEASSRRKAAEWEREVRIEIDR
                     RENQVTLKTEYPGHRTRNNLFDFLYSDDSHPRGQVNYRIRIPESGTVSLHTTSGNCYV
                     AHAKDRVELNSTSGDLEIIGIDGAVLIETTSGDVIVREVSGNLDLATTSADLTIADAR
                     GALRIKSVSGDIHLSRLTGDLTVESTSGDVQADGVQGEVRVTTVSGDVTVSGQTGGCY
                     LTTISGDIETKIAQLTGASALATTSGDIHLFLPEEADAALSIATRGGEIETHLPLALQ
                     SVKRHQVEGTLGRGSHKLEMKSTSGDIAVTGF"
BASE COUNT          849 a         1104 c         1140 g          794 t
ORIGIN      
        1 tgtctccgga tcctctttca ggcgctcggc cgggtcaagt ttggcggttg cctttttcat
       61 ctttaatacg ccggggaaaa gactctgttg ggtggagatt ccactcccta tcactggtgg
      121 gtttctcact tccccacacc gaggagaata tacacccttt tttacggagc gcacgccatt
      181 tttgttcccc gctctcggga ggagagtggt cgtccgaccg cgctccaaat aatgaaaccc
      241 cgtcccagat gacggggttt cattatttcc tgcgtctggg tagaattatt taacgttcga
      301 taaggacgta gcgcatcggg ttctgcggca tggtgcgcag gtggacttcg tagtgcaggt
      361 gcgggccggt ggagtagccg gtggaaccga ccgcgccgat caactgccct ttggtgaccc
      421 tttgtccggg cgtcacccgg atcgccgaca ggtgaccgta gtaggtctca aaaccgcggc
      481 cgtggttaat gataatcgcc ttccccagtc ccgagcgcca gccggcgtag acgatccgcc
      541 cggtggcgga cgcttgcacc ggagtacccc agggagcggc cagatcgact ccggcgtggt
      601 gttccgactg gccggtgaag ggatcgctgt gcaaaccaaa tccccgggtc agacgccccg
      661 gcacgggcag gatggagggg gtgtggtcca gccggtcgcg gcggaccaca aacttgtcgt
      721 aaatttgatt cagcgcttcg cgttgctgga gggtggtggc caggagcagg tcgagatcgg
      781 cctccgcggg caagggttgg ggcggcagct tgagctccgg caattccgcg gagcgcggcg
      841 tacggccgcg ttcggccaag cctaagaagc gttccacttt cttctcgtgc tccagaatga
      901 gtaaaatgtc ggtcttcagt tgatgggcca gcgcctgagc gtcaaaaaac ttgctctcca
      961 tgtgggcatt ctgcttggaa agctcggcaa ttttctggtt gtcgtaaatc ttcttgatgt
     1021 agttgaccga aaagaagacg tcggaaatga cgaacagaaa caggaagaag gcgggcaaaa
     1081 gaatcacgcc gaggggaatg gagagccgca gggctttccc cttggggtag ggaacgatga
     1141 gcaccgaaat ccgtttcttc agcatcaggc ctccgcggag acccgcgtct ccgatcgtcc
     1201 gggcgcgggt caaaagtccc gccctacgga atttcaccaa catagacctg gttggagaat
     1261 ttgtcaaggt attgttcacc ccattgtagg acctccttgt gtccggggcg ggagggtcag
     1321 gcatccggcg acgtgggctc gtcacttaat gagtgaaaac tcccgccgga gaatcttgtt
     1381 gaccacgcgg atgaatttct ccgcgttcaa gttctgcaaa ctgtacaggt gccaggcggt
     1441 cttttcggga atcttaaagc gtcgtttccg gcgtctgttc atggttctca cctcgactat
     1501 tggtcaccga accggggcgg gaaccactgg cacctttcga ccaccttcca ctcccaggta
     1561 tcggcaggcc gtctcaatcg cttaatgacc agcgcttatc cccgcaattg ccgttccgga
     1621 ctttattctg cgccagatgc gaaccggggt ccggtaagta cgtgtcattc ttagagatgc
     1681 ggaatgctta cccgaatctc caagaacgaa aaattcctct ttctctcgcc tctcacccac
     1741 agaatatggt gattgtccaa aggccggtaa tggcggcaat ttcaaactta tgcaaaatga
     1801 ccttactcgc tgccggtcaa agattgagca gtgacgtgat caaccttcca acggatagag
     1861 tgaccgttgc caaaacgatt gccttgctct tcagggcgcg aaaagcgaag gcccggtcat
     1921 gagctccgcc tccttctctt gaactacctc ccatcacggt ttccgtagca acgctctaga
     1981 ccttgccgct ccggcaaaaa gttgtatggg ttggcagcat cgtttatctt ggctcgactt
     2041 tggcgaaacc tcaggcggcc gcgcctcgta cgaaagagat gcaggccaga gaaaccgccg
     2101 agagcgatca ggccctggtg gtgcaggctc tggcaggtga aaagaaggcg tttgccgcgc
     2161 tcctggagcg gcaccggcag gcggtcttcc acatcaccta ccagatcgcg cacaaccagg
     2221 aagaggcgcg cgacctggtg caggaaacct ttatgaaggc ctttgcaggg ctggcgacct
     2281 accggtccca gttccgcttc accacctggc tctaccggat cgccgccaac tgcgccatcg
     2341 acgctctgcg gaaaaagaaa atcgacgcgc tctcgctgga ccagcccctg gaaacgggcg
     2401 agggtacggt ggccttcgag gtggccgacc tggcggccga ccccgaggcg gaactggtcg
     2461 ccgaacagag gcggatctcc atcggtgagg caatcgacgc cttgcccaag aagtaccggg
     2521 aggtactggt tttccgccac cgggaggaaa agtcgtatga ggagatcagc gacattttga
     2581 aaataccggt gggaacggtg aaggccagga tattccgggc ccgggagtta ctgaaaaaaa
     2641 aattgaaaca ctaccgtccg tagccggaac gaaagcgaga gccaccgatg aatttcaggg
     2701 acaagttgac gcagcggaac gttttccggg tcgcaaccgg cgccatcgca gcgacggcgc
     2761 ttttgctctt ttcttttgcg gagagccaga gccgtcagta cacctacgaa ttccacaaag
     2821 agcttccggt ttcccccggg ttcgttttct cgctgaccga tccgatcggg gatatcgttg
     2881 tagagggata cgccgggagg tcaatcgaga ttgctgcccg aaaagtggtc gaagcctcca
     2941 gccggagaaa agccgcagag tgggaacgag aagtgcggat tgaaatagac cggcgggaaa
     3001 accaggtcac cctcaaaacc gagtacccgg ggcaccgcac ccgcaacaac ctttttgact
     3061 ttctctatag cgacgacagc catccccggg ggcaggtgaa ctaccgcatc cgtattccgg
     3121 aaagcggcac ggtcagcctc catactacct ccggaaattg ctacgttgcc cacgccaaag
     3181 atcgggttga actcaacagc acctccggcg acctcgaaat aatcggcatt gacggtgctg
     3241 tgttaataga aaccacctcg ggtgacgtta tcgtgcggga ggtttccggc aatctcgacc
     3301 ttgccacaac cagcgccgac ctgaccattg cggacgcgcg gggcgccctg cggatcaaaa
     3361 gcgtctccgg cgatattcac ctatcacgac taaccggcga tctcacggtt gagagcactt
     3421 ccggtgacgt gcaggcggac ggggtgcagg gtgaagtacg ggtaaccacc gtttcggggg
     3481 acgtcacggt cagcggtcaa acggggggct gttaccttac aactatttcc ggggacattg
     3541 aaaccaaaat cgcgcaactg accggtgcct cggccttggc cacgaccagc ggcgacattc
     3601 acctcttttt accggaagag gccgacgcgg cactttccat cgccacccgg ggcggtgaaa
     3661 ttgaaacgca cctccccctc gctctccaaa gcgtgaagcg ccaccaagtc gagggcacgc
     3721 tgggccgggg aagccacaag ctggaaatga aaagcacctc cggtgatatt gccgtgaccg
     3781 gtttctaaag gatgaacgcc atgtcggaat tatggaaaaa tctgattctc gacaatgatc
     3841 ggggggatgc gtgaaccggc ggactgtacc ctggctactg cttttcg
//
LOCUS       DBNGZD010000347         2999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1092151, whole genome shotgun sequence.
ACCESSION   DBNGZD010000347 DBNGZD010000000
VERSION     DBNGZD010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1092151"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..992
                     /locus_tag="AC16GM_09230"
     CDS             231..992
                     /locus_tag="AC16GM_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR2923888.1"
                     /translation="MEKSFKPAIAEGIASFIFVCCAAGVICANAMTQNAIGWVGIALA
                     NAFAFTALVAFTSHISGGHVNPAVTIGNLFSGRIDFPKATFYIIAQLVGAILAGFVTR
                     TIFPANVWQTVSLGAPILNNPVTTGGAIFIEFLFTCFFVMVAIGCTADDRAPKNLAPL
                     AMGFALGFATLVIGPLTGAGLNPARAFGSAIGSGNWTNQVPYWIGPVLGGICAPILYR
                     ICGGQITKMVSSPWADFESTPTAPEPKVTVKSVNR"
     gene            complement(1352..2917)
                     /locus_tag="AC16GM_09235"
     CDS             complement(1352..2917)
                     /locus_tag="AC16GM_09235"
                     /inference="COORDINATES: protein motif:HMM:NF039629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HIR2923889.1"
                     /translation="MRLLIFFLCCGTAAPASAQISITNLGEFQAGRLPGGLDSARRAT
                     YYGDLTVDYFNAKLIPKVQLQTRFSLTGFQAPTGRSDFVKLYQKLLSARHRNFEVQAG
                     NYFATFGRGMLLRAFQLPGFIYEDRVERIRHEAIRDLFGFRATVTLPLLEAAFVQGKP
                     QDALVTPTGRRTGSLWGGDFRVKPFPRSEALRLGGSFLHLKSQGESEPVASGVLEANP
                     LQFFGEKVSAKWGADLYVEYALKHPTFQRFFSFGWRDSAYGFYGSMNLSYARVGLSFE
                     YKDYHNFLLRAGGPVNDPPALIREQSPILLNRETHVLVPEDERGFQIELAAGITTALR
                     VVANLSRAENEIKIGRRKKPVFLERYLEVDYHPLPNWSFRGFADLAQDQFRLQKRRLS
                     GGLAADFDLTKTHSVGFDLQAQKITRDLPFAFIKPDFTNLYLGLKYAWSPLLTVTLSG
                     ERSDDPFEDTDTEKGPHDWVNVGLSLRIADSHQIDLFYGRRRGGPACDHGYCISVLPF
                     KGFEMRMTNNLWF"
BASE COUNT          631 a          828 c          953 g          587 t
ORIGIN      
        1 aatcacccta cggtatatta atgtgggggc tctaaacccg catctggagc ggggggagac
       61 ccctatcaag tgttaggggg ggtggccgat actttcactg gttcgactgc attcaccatc
      121 cgcctcaggc ggacagttgg acttgtagac cgggcgaaga gttattcccg tccgccttag
      181 gcggattggt atcgacgtaa acaggagcga aacacaagga gggagggagt atggagaaat
      241 cgttcaagcc ggccatcgca gaggggatag cgagtttcat cttcgtctgc tgtgcagccg
      301 gagtgatctg cgccaatgcg atgacgcaga atgcgatcgg ctgggtgggc atcgccctgg
      361 ccaacgcctt tgcctttacg gctctggtgg cgttcacctc acacatctcc ggcgggcacg
      421 tcaacccggc ggtgacgatc ggcaacctct tttcggggcg gattgatttc ccgaaggcga
      481 ccttttacat catcgcgcag ttagtcgggg ccatcctggc cggtttcgtc acccggacga
      541 ttttcccggc caacgtctgg caaacggtga gcctgggggc gccgattctg aacaacccgg
      601 tgaccaccgg cggggcgatc ttcatcgagt tcctctttac ctgcttcttc gtcatggtcg
      661 ccatcggctg cacggcggac gaccgggcgc cgaagaacct ggctccactg gcgatgggat
      721 tcgcgctggg atttgccacc ttagtgatcg ggcctctgac gggcgcgggg ctaaacccgg
      781 cccgggcgtt cggctcggcc atcggctccg gcaactggac caaccaggtg ccgtactgga
      841 tcgggccggt tttaggcggc atctgcgcgc cgatccttta ccggatttgc ggcggacaga
      901 ttaccaagat ggtttccagc ccctgggccg attttgagag cacgcccacg gctccggaac
      961 cgaaagtaac ggtgaagtcg gtgaaccgtt agttcccaac ctatcacagg gatgtgaaag
     1021 tgtggggcac ccggcccccc cggatcgatc catacggacg tacgggaggg ccgggttttt
     1081 tatttcccgg gagagtttca cagcagatga agcagattga gcagatggga atactaaccg
     1141 gctcagggac gagctcatac gaggcgcacc gaccggtcta ccagcgtagc cgaggtttgt
     1201 cacctcggcg gctctcctaa gctgtcatcc tacgtcaggc cacgctcgta cggagcgccg
     1261 aggatgtaat cctcggctac gaaacagcgg agagacttgc tgacccctcc cgtgccctcc
     1321 tctcggaggg gagggggcat caaatgagga atcaaaacca gaggttgttc gtcatacgca
     1381 tttcaaagcc cttaaagggc aggacgctaa tgcagtaacc gtggtcgcag gcggggccgc
     1441 cgcggcgacg gccgtagaag agatcaatct ggtggctatc ggcgatccgc agactcaggc
     1501 cgacgttaac ccagtcgtgc ggccccttct cggtatcagt gtcctcaaat gggtcgtcgc
     1561 tccgttcgcc ggagagggtt acggtcaaaa gcggcgacca ggcgtacttc aaaccgaggt
     1621 agagattggt gaaatcgggt ttgatgaagg cgaagggcag gtcgcgggta attttctgcg
     1681 cctgtaggtc aaagccgacc gagtgcgttt tggtcaaatc aaagtccgcc gccaagccgc
     1741 cgctcaaacg gcgcttctgg aggcggaatt ggtcctgcgc taagtcggcg aagccacgga
     1801 aagaccagtt ggggagcggg tgatagtcta cttccaaata gcgctccagg aaaaccggct
     1861 ttttacgacg gccgatcttg atttcgtttt cggcgcggga gaggttcgcc actacgcgaa
     1921 gcgcggtggt aataccagca gcaagttcaa tctgaaatcc tctttcgtct tccgggacta
     1981 agacgtgggt ttcacggttc aagagaatcg gcgactgttc gcggatcaaa gccggaggat
     2041 cgttcaccgg gccgccggcg cgcaaaagga agttatggta gtctttgtat tcgaaagaaa
     2101 gccccacccg ggcgtacgac aggttcatgc tgccgtagaa gccgtaggcg gagtcgcgcc
     2161 agccgaagga gaagaaacgc tggaaggtcg gatgttttaa cgcatactcg acatacaggt
     2221 cggcgcccca ttttgcggag actttttccc caaaaaattg caaaggatta gcttcgagga
     2281 cgccggaggc gaccggctcc gactccccct gacttttcag atgcaggaac gacccgccga
     2341 gacggagcgc ttcgctccgg ggaaagggtt tgacccggaa atcgccgccc cagagggaac
     2401 cggtgcggcg gcccgtgggc gtgaccaggg cgtcctgggg tttgccctgg acgaacgcgg
     2461 cttccagcaa aggcagggtg accgtggccc ggaacccgaa gaggtcgcgg atggcttcgt
     2521 ggcggatacg ctcgactctg tcttcgtaaa tgaagccggg aagttggaaa gcgcgtaaca
     2581 acatcccccg gccgaaggtg gcaaagtagt tacccgcctg tacttcgaag ttacggtgcc
     2641 gtgcggatag tagtttctgg tacagtttca cgaagtcgga gcggccggtg ggggcctgga
     2701 aaccggtgag cgagaagcgg gtttgcagtt gaactttcgg gatgagtttc gcgttgaagt
     2761 agtccaccgt caggtcacca tagtaggttg cacgccgggc cgaatcgagg ccgccgggca
     2821 ggcggccagc ctggaattca ccgagattgg tgatggaaat ctgagcgctc gccggtgcgg
     2881 ccgtcccgca gcacaaaaag aaaatcaaca gtctcaccgt cccagagcat ctgaagacaa
     2941 aaaaagggac tcggctgagc ccccgctcta tgtcggcgag cagtttccgt tcacggcga
//
LOCUS       DBNGZD010000348         2476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_402717, whole genome shotgun sequence.
ACCESSION   DBNGZD010000348 DBNGZD010000000
VERSION     DBNGZD010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_402717"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /gene="xerA"
                     /locus_tag="AC16GM_09240"
     CDS             <1..860
                     /gene="xerA"
                     /locus_tag="AC16GM_09240"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HIR2923890.1"
                     /translation="VSKFLDYLAKERRFSPNTVTAYRNDLVQFLQSTHGALDFSSRTL
                     EHFLGRLKKQGYDNHSLSRKISTLRSFVRFLQRRMKSVVPASVDFPTIKTQKRVPEFL
                     SVEKTRQLLDSIEVHDLWSARDRAILELFYSTGIRLSELAGLKDHEVDFSRGEITVHG
                     KGAKDRVVPVGQVALGALRTYLALRSNFRKNGRFGYLFINRQGGQLTARSVARIVVKY
                     GLPYAEGRPLGPHTFRHTFATHLLDRGADIRAVQELLGHRRLSTTQIYTRVTVEKLKK
                     AYDQAHPRA"
     gene            835..1395
                     /gene="hslV"
                     /locus_tag="AC16GM_09245"
     CDS             835..1395
                     /gene="hslV"
                     /locus_tag="AC16GM_09245"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR2923891.1"
                     /translation="MTKHILVPEEQKWRSTTIVGIIHNGKVALGGDGQVTFEDTILKH
                     NAKKLRRLHNGTILAGFAGASADAFALFDRFESKIEEYKGNLPRAAVELAKDWRTDRY
                     LRRLEALLAVLDKEHALIISGNGDVIEPDDKIVCIGSGGAYALAAARALIQHAKLDAK
                     TIVKRSLEIASEICVFTNDKISIEEL"
     gene            1431..1862
                     /locus_tag="AC16GM_09250"
     CDS             1431..1862
                     /locus_tag="AC16GM_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR2923892.1"
                     /translation="MERYRHTQIGYVTIGATAPVFIFFVYVMTFIPFNGYVVAGFLTL
                     GLVSGLFASLTVLIDGEHLKIRFGPGVIRKKFPLREITVCRVVKNPWYYGWGIHLTPH
                     GWLYNVSGFSAVEIAMRSGKKYRIGTDVPQELERAIKQAIS"
     gene            1959..>2476
                     /locus_tag="AC16GM_09255"
     CDS             1959..>2476
                     /locus_tag="AC16GM_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923893.1"
                     /translation="MKTSIHSRRWCKFFFLFAVISFSIPKVYAQRDTTQIKEPQNSLR
                     SGAWALLFEVGSNFTLEAFQGFTISAKRHFSDKRALRLGLGISGSVSLDDEVIDRQLK
                     GDTVTFQEDQEGDLNNESVDLIVQHLWYANPKAEVNFFFGVGPLLRFSHSKSETDATE
                     ISGGITTLINSTS"
BASE COUNT          554 a          688 c          691 g          543 t
ORIGIN      
        1 cagtcagcaa gtttctcgat tatttagcca aggagcgtcg cttttcccct aacacggtga
       61 cggcctaccg caacgacctc gttcaatttc tccaatcgac ccatggcgcg ctcgacttca
      121 gcagccggac gctggaacat ttcctcggtc gcctaaagaa acaagggtac gacaaccact
      181 ccctttcgcg gaagatttcg accctccgct cttttgtccg ctttctccag cggcggatga
      241 agagcgtcgt ccccgcgtca gtcgatttcc ccaccatcaa aacccagaag cgggtgccgg
      301 aatttttgag cgtcgaaaaa actcgtcaac tcctcgattc catcgaagtg cacgacctct
      361 ggtccgcccg tgacagagcg atcttagaac tcttctactc caccggcatc cggctgtcgg
      421 agttggccgg gctgaaagac catgaagtcg atttcagccg tggggagatc accgtgcacg
      481 gcaaaggagc caaagaccgc gtcgtgccgg tggggcaggt ggcgttaggg gcgctgcgga
      541 catatcttgc cctgcgcagc aattttagaa agaacggacg attcggatac cttttcatca
      601 accggcaggg agggcaactg accgctcgca gcgtggcgcg gattgtcgtc aagtacggct
      661 tgccgtatgc tgagggccgt cctttgggcc cccacacttt ccggcacacc tttgccacgc
      721 atctgttgga tcgcggggcg gacatccgag cggtgcagga gttgttggga caccggcggc
      781 tttcgaccac gcagatttac acgcgggtaa ccgtggaaaa actgaagaag gcctatgacc
      841 aagcacatcc tcgtgcctga agaacaaaag tggcgctcga ccaccatcgt cgggataatt
      901 cacaacggca aggtcgcgct cggcggcgac ggccaggtga cctttgagga caccatcctc
      961 aagcacaacg ccaaaaaact ccggcgcctg cataacggga cgattttagc gggtttcgcc
     1021 ggggcgtcgg cggatgcttt tgcgcttttc gaccggttcg agagcaaaat cgaagagtac
     1081 aaaggcaacc tgccccgcgc cgccgtcgaa ttagccaaag actggcgcac agaccgctac
     1141 ctgcggcggt tagaagcgct tttggccgtt ttggacaaag agcacgcgct catcatctcc
     1201 ggcaacggcg acgtgattga gccggacgac aagatcgtct gcatcggctc cggcggggcg
     1261 tatgctttgg ccgccgcgcg ggcgttgatc cagcacgcca aattggatgc caaaaccatc
     1321 gtcaaacggt cgctcgagat tgcgtcggag atttgcgtgt ttaccaatga caagatttcg
     1381 attgaggagt tgtaagggtt taacgtccgc acccgcccgg caataatcgc gtggagcgct
     1441 accggcacac gcagatcggc tacgtaacca tcggggccac cgccccggtg ttcatcttct
     1501 ttgtgtacgt gatgaccttt atccccttca acgggtatgt cgtggcggga ttcttgaccc
     1561 tgggactcgt ctcggggctc tttgcctcgc tgacggtctt aatcgacgga gagcacctta
     1621 agattcgctt tgggccgggg gtgatccgga agaaattccc gctcagagaa attaccgtct
     1681 gccgggtggt gaagaatccc tggtactacg gctggggcat ccacctgacc ccgcacgggt
     1741 ggctctacaa cgtttccggc ttctcggcgg tggagattgc catgcggtcc gggaaaaaat
     1801 accgcatcgg gacggacgtg ccgcaggagt tggagcgggc gatcaagcaa gcgatttctt
     1861 aaaagcgtac agggatgtta tattgttaag gctacaggcc tcatcggcta gtgtgttatg
     1921 atttgtgctg agggaagtcg aaggaaaggg gcaccaccat gaaaacgtca attcattccc
     1981 ggcgatggtg caaattcttt ttcctttttg cagtgatttc tttctctatc ccaaaagttt
     2041 atgcacaaag ggatacgact caaatcaaag aaccccaaaa cagtcttcga tccggcgcgt
     2101 gggcgctcct gtttgaagtc gggagcaatt ttacccttga ggcctttcag gggtttacca
     2161 tttcggccaa gcgtcatttt tccgacaaaa gggctctgcg gctcggccta ggcatcagtg
     2221 gctcggtctc gctggatgac gaggtgatcg acagacagct gaaaggcgac accgtcacat
     2281 ttcaggagga tcaggagggc gacttaaata atgaaagtgt agacctcatt gtacagcatc
     2341 tctggtatgc aaatcccaaa gccgaagtga actttttctt tggcgtagga ccgctcctgc
     2401 gtttttccca cagcaaatct gaaactgatg caacggaaat ttcaggtgga atcaccaccc
     2461 tgatcaactc tacctc
//
LOCUS       DBNGZD010000349         2256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_55140, whole genome shotgun sequence.
ACCESSION   DBNGZD010000349 DBNGZD010000000
VERSION     DBNGZD010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_55140"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..483)
                     /locus_tag="AC16GM_09260"
     CDS             complement(4..483)
                     /locus_tag="AC16GM_09260"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR2923894.1"
                     /translation="MSTSGKTVAPRATESETSAGSPSAEFPVGRRNRFYSAYLPAILY
                     ALLIFGLSSIPNLQAPNLGIGPVDKLVHAVEYAIFGVLLSRAVTGFPRLRNHILWRPL
                     VIAVGGLYAASDELHQLLVPGRSAELADWVADVAGLVIGLFILLPLVHRLLKKKVKT"
     gene            864..1736
                     /locus_tag="AC16GM_09265"
     CDS             864..1736
                     /locus_tag="AC16GM_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2923895.1"
                     /translation="MKRFSAKGSAERCVFLVLTAFRTRLILDTKMKTTPTHDSKQPLK
                     GKVALVTGGSRGIGRACALKLAEKGARVAIGFFKSREKAKKTQAEIAALGGDCQTVRV
                     NLAKTDSVEKMFAEMKAHYGRLDILVCNAAMGIFGLTLDISLEDWELTMDANTRSFFL
                     CAREAVKLMLPHGGGKIVGLTSYGSQRFIPAYAAMGVAKAGIETLTRYLAVELVPKGI
                     NVNCVSGGPVDTDSLRLIPGADKVVEESVKRTPAGRIGQPEDIANIVAFLCTDEANWI
                     CGQTIVADGGVSLI"
     gene            2009..2218
                     /locus_tag="AC16GM_09270"
     CDS             2009..2218
                     /locus_tag="AC16GM_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923896.1"
                     /translation="MKKMVMAVLTLVLGLSFVAGILISRAEAACYTQCQYCPNCHLLQ
                     CCNGVCVDTGKSCGSFCRFVDCNPP"
BASE COUNT          505 a          644 c          645 g          462 t
ORIGIN      
        1 ttcttacgtc ttgacttttt tctttaacag ccggtgtacg agcggcagga ggatgaacaa
       61 cccgatcacc aggccggcca cgtccgccac ccagtcggcc agttccgcag agcggcccgg
      121 cacgagcaat tggtgcagct cgtcgctggc ggcgtacagt ccgcccacag caatcacgag
      181 cggccgccag aggatgtgat tgcggagccg cgggaatccc gtgacagcgc gggagagcaa
      241 aaccccaaaa atggcatact ccaccgcgtg cacaagtttg tccaccgggc caatacccaa
      301 attcggcgcc tggagattgg gaatcgaaga cagaccgaag atcaacaggg cgtaaaggat
      361 tgccggcagg taggcggaat agaaccggtt ccgccgaccc acagggaatt ctgcgctggg
      421 cgagccggca ctcgtctccg actcggtcgc gcgcggtgcg actgtcttgc cgctggtgct
      481 cacttcccca gcatacgcgc cgatcgattc cccggcaaat aaaaaaggcc ggagtcgtta
      541 cctccagcct tcactcaaag aagaagcggg tttagcgctt tttccccttc ttcgcttttt
      601 tctttttttt catcagggca ccctcacctc cttcggcaca gcgggttcct ccagattgcc
      661 ccgtccgagg cggggcacaa aaaaacccga gtcacgctcg ggttcgtctg ccaatgatcg
      721 gatactcgac cacgcactta ccaccgaaat ttttcaatcg acaccgcatt tacgttaaag
      781 taacggacgc tgcaaaaatt tgtcaagaaa aattttgcat tttgagaaat tattttcgtt
      841 ccgccctttc gtgcgcgtaa gcgttgaaga gattttctgc gaaaggaagt gctgagcgct
      901 gtgtttttct cgtcttgact gccttccgca cgcggcttat actcgacacc aaaatgaaaa
      961 caactcccac acacgactca aagcaaccgc tgaagggaaa agtggcgctc gtgaccggcg
     1021 gctcgcgcgg gatcggccgc gcctgcgcgc tcaagttggc cgaaaagggc gccagggtcg
     1081 ccatcggttt cttcaagagc cgggagaagg ccaaaaaaac gcaggccgaa atcgccgcgt
     1141 tgggcggcga ttgccagacg gtgcgggtca acctcgccaa gaccgacagt gtcgaaaaga
     1201 tgttcgccga gatgaaagcg cactacggcc ggctagatat tttggtctgc aacgcggcga
     1261 tgggcatctt cggcctgacg ctcgacatct ccctggaaga ctgggaactg acgatggacg
     1321 ccaacacccg gagtttcttt ctctgcgcgc gggaggcggt caaactgatg ctcccccacg
     1381 gcggcgggaa gattgtgggg cttaccagtt acggctccca gcgcttcatt ccggcctacg
     1441 ccgcaatggg cgtggctaag gcagggatcg aaaccctgac ccgctatttg gcggtggagt
     1501 tggtccccaa ggggatcaac gtcaactgcg tctccggcgg cccggtggac accgactccc
     1561 tgcgcttgat cccgggggcc gacaaagtcg tggaagagtc ggtcaagcgc acacccgccg
     1621 ggcggatcgg ccagccggag gacatcgcca acatcgtcgc cttcctctgc accgacgaag
     1681 ccaactggat ttgcggccag acgattgtgg ccgacggagg agtatctttg atatgaggtt
     1741 gcaaggccac cctgagcgaa atcgaagagt cgcggacggc gggatttctt tttgacaaat
     1801 tcctgccggc cgtatttttt tcgaccgaag taaaaaaaat aacagtccct ggttacgggg
     1861 tgagacggtt gagaaaagag acgctttatc ttttgatgat tcaatgcggc tgtagccgca
     1921 tattcccggg tatttcggcg agccgaccag aaagcgggtc ggctttttta cgtccaaaac
     1981 ctcattcgaa aaaaggaggt gagtaggaat gaaaaagatg gtcatggcgg ttcttaccct
     2041 ggtcctgggg ctctcgtttg tcgccggcat cctgattagc cgggcggaag cggcctgcta
     2101 tacgcagtgc caatattgcc ccaattgcca cctcttgcag tgctgcaatg gggtgtgtgt
     2161 tgacacgggg aagtcgtgtg gctcgttttg ccgctttgtc gactgcaacc cgccctaagc
     2221 cacgaatcca cggaaagaaa acccctcaaa gaatga
//
LOCUS       DBNGZD010000350         6004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1722624, whole genome shotgun sequence.
ACCESSION   DBNGZD010000350 DBNGZD010000000
VERSION     DBNGZD010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1722624"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="AC16GM_09275"
     CDS             complement(<1..367)
                     /locus_tag="AC16GM_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR2923897.1"
                     /translation="MTKGIFLWMSFLFWATAATPVQSQPGRTAYVVNGLAETLGKINL
                     ENGAVVESIATLGVVPNQIVLRGRRGYVVNSISSNVQIINLATGVTEGTVELGPSRNP
                     WNLALADTETAYVTNFAAGT"
     gene            complement(385..2586)
                     /locus_tag="AC16GM_09280"
     CDS             complement(385..2586)
                     /locus_tag="AC16GM_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2923898.1"
                     /translation="MQLGFVCGVVFFSLSPEPITGQSTDKLQGFVRDSQSSAPVAGAV
                     VELVGTNYRDITDERGFYRFEHLPVGTYSVVVRSIGYRTRRIEQVGVVQDVTARLDIT
                     LEAKPIPLPPQEVKGLGPASKFSSTQDVTIISHKEIERSGAGSLAELFQTVPGLFVYQ
                     NGSASGEKALSVRGSAANQVVILVNGQRLASGATGTADLAGIPLAIVERVEVVKGGQA
                     ARIGPDALAGVINIITRTVSGKRTVVEVTGRVGSFRTQEGEVTFGRQLTERWQVTGSA
                     WAQATAGDFPYRDSLGQVFLRENAAREAANFYASASANLFRGGNLQFSLQWYETGNGI
                     PGALLQFTPAATRREQRTLFSAAWNQPVTDHFQMELAGSLQRYRQVFQNSETPDLFSR
                     YWAATRTDLDQISGRWNWSPLRDLVLSFGSEYLSGQFTSTDLLRPHLSLGKAPRRETI
                     GFFLAGEAKLEHPWPPLVDLAVLSAAVRRDKTSGFSGATNPHVGLLLARGDAWKITAK
                     GNWQRAYRNPALVDLFWKEDALAVGNPQLKPERSSDWDAGGEVRLPLLGQVTVGDTWF
                     GSDVRDLVVWQRRFDGKYTPQNLIRAQISGREESFGWESRDEKIKAGLQHTYSRAVDR
                     SGFRPHEGKQLVFRPRHVLRAWGQFTWRGWSLASSYRWVDRRFIREANTKSLPPYHLI
                     DLTCGVTFPRKAGWEIRSAFKVTNLTNQAYELLERQPMPGREWRFELRFKK"
     regulatory      complement(2798..3006)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3075..3803)
                     /locus_tag="AC16GM_09285"
     CDS             complement(3075..3803)
                     /locus_tag="AC16GM_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923899.1"
                     /translation="MSTSSSPEPRPPARPLHRKAGWPRRLWRILAAPRPGRVFVYFLV
                     FYLALYPLYQVINQPYTAFVVKITSSVFHVVTTRHAVPSVSMDGNGIKVKVLITPRGG
                     LPDPTAKVFTGLRTAYDLGNYIVFYLALLLATSCQACRRENLKAFLGGLVWLLAAHVF
                     DLSLYATFGYHEAAAVLAESRQYAYHSTLHRIVSFLLDAEVAIFPRAVPLVLWIFFFR
                     KRLGAMARMWRERRLLRRTGKTPV"
     gene            complement(3781..4350)
                     /gene="xrtH"
                     /locus_tag="AC16GM_09290"
     CDS             complement(3781..4350)
                     /gene="xrtH"
                     /locus_tag="AC16GM_09290"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HIR2923900.1"
                     /translation="MSKRSRQEKNLPPHPSKRSITGRLIYFIREHKKLFRMYLFFGLA
                     LLALFGVVTLLDKTPFPGWLNRRLAHASGTVLRLIDPPVTVAGEYIGMGNFGAAIAEE
                     CNGIYATVILLAGFLAFPAPWIKKFIGVALGIVALQIINVVRIVVLFLIGKHFPSFFE
                     ASHLYVAEFVVIACGAGLWLLWYVYIVKP"
     gene            complement(4547..5026)
                     /locus_tag="AC16GM_09295"
     CDS             complement(4547..5026)
                     /locus_tag="AC16GM_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923901.1"
                     /translation="MRRLVTAVLTLVFVGLMVSPVLAAGDSVRVTITTGSHTIAVTSI
                     TFTINSSPVSVTPVPPSTGPGQVVSRTYAVAAQPTACTMPFLMDGIGHSFVGNPLTYD
                     TDLTLPFHGSPDSKVRFTFISVLPTLTQWGLVIFGMLLAGTLTSIYLLRRRKARLAT"
     gene            complement(5581..>6004)
                     /locus_tag="AC16GM_09300"
     CDS             complement(5581..>6004)
                     /locus_tag="AC16GM_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2923902.1"
                     /translation="HERGRYIAAGGGEILKITGVAAAEYVEAQAADRTGHPVTIGLAK
                     PPLPVQFSLSQNRPNPFNPQTTIEFSLPLSSPVRLVIYNVAGQVVRTLLESDLPAGVH
                     AVVWDGRNDQGIEVSSGIYFYQMAAGSFQETKKMVLLK"
BASE COUNT         1335 a         1756 c         1709 g         1204 t
ORIGIN      
        1 gcgtgccggc ggcgaagttc gtgacgtagg cggtttcggt atcggccaag gcgaggttcc
       61 agggattgcg gctggggccc agttccaccg tcccctcggt gaccccggta gcgagattga
      121 tgatctgcac gttggaagaa atcgaattga cgacgtagcc gcgccttccg cgcaggacga
      181 tctggttggg cacgaccccc agagtcgcga tactctcgac gacggcaccg ttttccaggt
      241 tgattttccc caacgtttcc gctagtccgt tcaccacgta agccgtgcga cccggctgag
      301 actggaccgg tgtggccgcc gtcgcccaaa atagaaaact catccagaga aatattccct
      361 tcgtcattat ccctcccttt tggtctactt tttaaacctg agttcaaatc gccactcccg
      421 gcccggcatc ggctgccgct ctaagagttc ataagcttga ttggtgaggt tggtgacttt
      481 gaacgcggag cggatttccc accccgcctt ccgcgggaaa gtgacaccgc aggtgaggtc
      541 aatcaagtgg taaggcggca gagacttcgt gttagcttcg cgaatgaagc ggcggtccac
      601 ccagcggtac gacgaggcca gcgaccaccc ccgccaggtg aactgtcccc atgctcgcag
      661 aacgtgccgg ggccggaaga ccaattgctt cccttcgtgc gggcggaacc cggagcggtc
      721 aacggcgcgg ctgtacgtgt gttggagacc tgccttgatc ttctcatcgc gactctccca
      781 gccgaacgac tcctcccgcc cgctgatctg ggctcggatg aggttctgcg gcgtgtactt
      841 cccgtcgaac cgcctttgcc agacgacgag atcgcggacg tcactgccga accaggtgtc
      901 tcccacggtg acctgcccca gaagcggcaa ccgcacttcg ccgccggcat cccagtcgga
      961 ggaccgctcc ggcttcagct gaggattccc cacggccagg gcgtcttctt tccagaacag
     1021 gtcgaccaga gccggattgc ggtaggcccg ctgccagttc cctttggcgg tgattttcca
     1081 ggcatcacct ctggcaagca aaagccccac gtggggattc gtagcgccgc tgaaaccact
     1141 ggtcttatcc cgccgtactg ccgcggaaag cacggccaga tcaacaaggg gcggccaggg
     1201 atgctcaagc tttgcttcac cggcaagaaa aaatccgatt gtttcccggc ggggtgcttt
     1261 tcccaaactc aagtgcggtc gcaagagatc ggtgctggta aactgcccgg agagatattc
     1321 actgccaaag gagagcacca ggtcccgcag tggactccag ttccagcggc ctgagatttg
     1381 gtccagatcg gtgcgagtgg ccgcccaata tcgggaaaaa agatccggcg tttcactgtt
     1441 ttggaaaacc tgacggtagc gctggaggga accggccaat tccatctgga aatgatcagt
     1501 gaccggttgg ttccacgcgg cactaaaaag agtgcgctgc tcccgccgtg tcgctgctgg
     1561 agtgaactgg agcagtgcgc cggggatgcc gttccccgtt tcgtaccact gcagggaaaa
     1621 ctgcagattc cccccccgga aaaggttggc gctggcactg gcatagaaat tggccgcctc
     1681 ccgggccgca ttctcccgca ggaaaacctg tccgagcgag tcgcggtacg gaaaatcacc
     1741 ggcggtggcc tgcgcccagg cgctcccggt gacctgccac cgctctgtca gctgccggcc
     1801 gaacgtgact tctccctcct gggtgcgaaa actccccacg cgtcctgtga cttcgacaac
     1861 ggtgcgttta ccggatactg ttcgggtgat aatgtttatg acacccgcca gagcgtcggg
     1921 ccctatgcgc gcggcctggc cccctttcac cacttccacc cgttccacta tcgccaaagg
     1981 aattccggcg agatcggccg ttcctgtcgc accggatgcg agcctttggc cgttcaccag
     2041 aattaccact tgatttgcag cgctgccgcg gacggaaaga gccttctctc cgctggccga
     2101 tccgttctga tagacgaaaa gaccgggaac tgtctggaag agctccgcca aggacccggc
     2161 accggagcgc tcaatttcct tgtgcgagat aatcgttacg tcttgcgtgg aggaaaattt
     2221 tgaagcagga cccagacctt tgacttcctg cggtggaaga gggatgggtt ttgcttccag
     2281 cgtgatgtcg agccgggcgg taacgtcctg gacgacgccc acctgctcga tacgacgcgt
     2341 gcggtagccg attgaccgca ccacgactga ataagtaccc accggcaggt gctcgaaccg
     2401 ataaaatcca cgctcatcgg tgatgtcccg gtagttcgtg ccaacaagtt ctacgaccgc
     2461 acccgctacg ggcgcgctgc tttgactgtc acggacaaaa ccctggagct tgtcggtcga
     2521 ttgtcctgta atcggttccg gtgacaggga gaaaaacacc actccgcaga caaaaccgag
     2581 ctgcaccagc cacccgcgga aaacagagtt acagcaacga tggtgccgcg ctgagacagg
     2641 ctttctgaac cgcaaaattt ctccctatga ataaaaaccc cggccgcctc accttcgtcg
     2701 gccgggggac agcgcataaa tatatccgca ccttacccgc gaaggtcacg gcggtcgctt
     2761 cctctctgat tggattggtt cagagaggca ctgcttgaaa tctggctggt ttcctgactt
     2821 gacggcttcg gtcctaccgg ccgcgccttc ccattccgcc tcaggcggga cagtggctgg
     2881 tgcggctttc gtagccgttt acagtagcgg ggctgtggcc gctttgcacg gccttcccac
     2941 ttagtcctcg tacgagtcca tacggatccg tagggatcgt accgacccat acgggaacac
     3001 cagattccgc tttgattcta tgctgcgccg gttaatctgt caaggctttt cctgcagggg
     3061 ggcaaaagag agtttcaaac cggtgttttt cctgtccgtc gcagcaaccg ccgctcgcgc
     3121 cacatccggg ccatcgcccc caaccgtttc cgaaagaaaa aaatccagag cactagcggc
     3181 accgcgcggg ggaagattgc gacttcggca tccaaaagaa atgagacgat ccggtgcagc
     3241 gtagaatggt aggcatactg cctggattcc gccaagactg ccgccgcttc gtggtagcca
     3301 aacgtggcgt aaagtgaaag gtcaaaaacg tgtgccgcca gaagccagac cagaccgccg
     3361 agaaaggcct tgaggttttc gcgccggcag gcctggcaac tcgtagccaa aagcaaagcc
     3421 agatagaaga cgatgtagtt ccccagatcg taggcggtgc gcaaaccggt aaacacctta
     3481 gcggtgggat cgggcaaccc gccgcggggc gtaatcaaca ctttgacctt gataccgttg
     3541 ccatccattg agactgacgg taccgcgtgc cgcgtcgtga ccacgtgaaa tacggaactg
     3601 gttattttga caacaaaagc ggtatagggt tggttgatta cctggtagag cgggtagagc
     3661 gccaggtaga aaaccagaaa atagacgaat acccggccgg gcctcggcgc ggccaggatc
     3721 cgccatagac ggcgcggcca tcccgccttg cggtggagcg gccgggccgg cgggcgcggt
     3781 tcagggcttg acgatgtaga cataccataa gagccacaac cccgcgccgc aggcaatgac
     3841 gacaaactcg gcgacgtaga ggtggctggc ctcaaagaaa ctgggaaagt gtttgccaat
     3901 caaaaacaaa acgacgatgc gcaccacgtt gataatctgc aatgcaacga tccccaatgc
     3961 cacgccgatg aactttttta tccagggtgc cgggaaagcc agaaatcccg ccaaaagaat
     4021 caccgtggcg tagatgccgt tgcactcttc ggcaatggcg gcgccgaagt tgcccattcc
     4081 gatgtactcg ccagcgacgg tcaccggcgg gtcaattagc cggagcaccg tacccgatgc
     4141 gtgcgccagc ctccggttca accagcccgg aaaaggggtt ttgtccagaa gcgtaacgac
     4201 gccgaagagg gccagaagcg ccaggccaaa aaagaggtac atccggaaca gttttttgtg
     4261 ttcacggatg aaatagatca ggcgtccggt gattgatctc tttgaaggat gtggagggag
     4321 atttttctcc tgccgtgatc ttttgctcat tatggctctg cgcgattgac tccgtctaaa
     4381 tctaccctta gggaaagcgg tctgtcaacg tccaataccc gacggtcagt gggcacgaac
     4441 cgtgaggggg agactgtcgc tactggcgca gcaactattt gaagtaagcg aaaagggccg
     4501 gtcagcagat ctgaccagcc ctgcttttcc tatgcccaat aacggcttac gtggccaggc
     4561 gggctttccg gcgccggaga agatagatgg atgtgagagt ccctgccaga agcattccaa
     4621 agataaccag cccccattgg gtgagagttg gcagcacgct gatgaaggtg aagcggactt
     4681 tggagtcggg cgagccgtga aacggcagcg taaggtccgt atcgtaggtc agcggattgc
     4741 cgacgaaaga atggccgatg ccatccatca gaaatggcat ggtgcaggcg gtgggctggg
     4801 ccgctaccgc ataagtccgc gagacgacct gccccggccc tgtggatggc ggcaccggtg
     4861 tcaccgaaac cgggctgctg ttaatggtaa aggtgattga ggtcacggct atcgtgtgcg
     4921 acccggttgt gatcgtcacc cgcacgctgt caccagcggc taaaacggga cttaccataa
     4981 gtccgacaaa cacaagcgtg agtactgcag ttaccagtct gcgcatgcta cctccgatga
     5041 ttgaaaaccg tttacttttc ctgcccattg ggcctcaaac catttgaggg ggtgcctgcc
     5101 caaagccttc cccgtgcgac caaccataac ctccctgact cctgtggtca agaaccgttc
     5161 cgctctgaga aaagttcccg ttaatatata gaaattgaaa aagtcccgct ttagggcggg
     5221 acttcttctc ttgcgcgcct cgcaataccc ccgaagaggg agtctgaggg tgcaggcgcg
     5281 tatcttagcc gttcaccgga actatagtaa caaacggcgt gccggacagc acggttgcgt
     5341 aaaaacttac ttaactcgtt actgcaccgg tggtttcgga aataaaatct cttcctcaaa
     5401 gggaaggata agcgcctgtt tcgcaattcc tgcgtgctgt gaatcccgac ctctaaagag
     5461 aatcctctta atccagcata tgccaacgct atgaaatgaa acgcactttc tgcgccccaa
     5521 attcgtattt acagcttttc cccacggaga cacggagaac acagagaaat ttttcagaaa
     5581 ttacttcaag agcaccatct tcttagtctc ctggaaagaa cccgccgcca tctggtagaa
     5641 gtagatgccg gaagaaactt caatcccctg gtcattgcgg ccatcccaga cgaccgcgtg
     5701 aactcctgcc ggcaaatcgc tttccaaaag tgtccgcacc acctgacccg cgacattgta
     5761 aatcaccagc cggaccggag aggaaagggg gagagagaat tcaatcgtgg tctggggatt
     5821 aaatgggttg ggccggttct gggaaagtga gaattgaacg ggcaaaggcg gcttggccaa
     5881 accaatcgtc acagggtggc cggttcgatc cgccgcctgg gcttccacat actcagccgc
     5941 cgccacgccg gtgattttta gaatttctcc gcctccggcg gcgatgtatc tcccgcgctc
     6001 gtgg
//
LOCUS       DBNGZD010000351         3138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1583927, whole genome shotgun sequence.
ACCESSION   DBNGZD010000351 DBNGZD010000000
VERSION     DBNGZD010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1583927"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="AC16GM_09305"
     CDS             <1..920
                     /locus_tag="AC16GM_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923903.1"
                     /translation="LFYASGYPEYPSNDPNKGEVSGLFGEYYDCEAFILRNWPSEHWS
                     GLHRGEFVAVRDDNNNGIPDDARVAVNDRTFGSGKSSDTTWTPMKKLLATNGILSGVD
                     ERWGGRVNPPDGRDGYPLTNARSDRPKKTPILDGKLSPGEWEEFATLNDRQLRAKIYF
                     QWDDSALYFAAEMSRPASLHLDIDAGNNGWFYGGDNYRLAVNSKETGATDSVAIYDWS
                     FFPRQPGFWNYEKIRPEELRSACTMGKRKCVIELAIPKNEGTGLRLQAGNEMNIRIGF
                     DWPGNKEGMVTLFEPHRFFGVKLVENNVR"
     gene            complement(996..1418)
                     /locus_tag="AC16GM_09310"
     CDS             complement(996..1418)
                     /locus_tag="AC16GM_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR2923904.1"
                     /translation="MSDEIPQDKIENSDVTQPQSPPATSPPPEAQAPLSLASLPPPTP
                     EQIREVIKEIVDPEIGISIVELGLIYGIEVRDHDVYVTATLTSPACPLGEVIKEQVEE
                     RSGSMPGVRYVYFELVWEPPWDPKTMCSEEAKLELGIF"
     gene            complement(1488..1952)
                     /gene="sufU"
                     /locus_tag="AC16GM_09315"
     CDS             complement(1488..1952)
                     /gene="sufU"
                     /locus_tag="AC16GM_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019912202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR2923905.1"
                     /translation="MSVRDDLYREIILDHFKSPRNRGKLPEPALKVEGANPLCGDEVE
                     LFILEKDGQLADVRIETHGCSISQAAASMMTEAIKGQSLADAEKLAGEFKEMMLGNGS
                     QPMPEDFGDLEALEGVKKYPVRIKCALLAWNTLLEGIKALKEHKPAQQVIME"
     gene            complement(1939..>3138)
                     /locus_tag="AC16GM_09320"
     CDS             complement(1939..>3138)
                     /locus_tag="AC16GM_09320"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIR2923906.1"
                     /translation="VNGKPLVYLDNAATTQKPQVVIDALVDYYERYNANIHRGIHTLS
                     EEATDAVEQARAKVAKFINAPSSSSVIFTRNATEAINLVAYSWGRKNLAAGDEIILSE
                     MEHHSNLVPWQMLAQSTSAKLKFISVTDDGLLDLDHIESLFTPCTKLVSVTQMSNVLG
                     TINPVREIADLAHRKGALVMIDGAQGVPHLGADVQAVASGGFDCDFLAFSAHKMLGPT
                     GIGILYGRQEILEKMDPFLGGGDMILQVWLDRATYNYLPWKFEAGTPNIADAIAFGAA
                     IDYLEKIGMDDIRQHEINLVDYTLKRLREIDDIKIYGPSKAEARGGVVAFNLPQMHPH
                     DLGTILNEEGIAIRAGHHCCQPLMRKLGVTGTARASFHVYNTRAEVDALVRGLHKAQE
                     IFSGVGT"
BASE COUNT          676 a          815 c          925 g          722 t
ORIGIN      
        1 cgctctttta cgccagcggg tatccagagt atccgtccaa tgacccgaat aagggcgagg
       61 tgagcggtct tttcggtgag tattacgatt gcgaggcgtt catcctgcgc aattggcctt
      121 cggagcactg gtccggcttg caccggggcg agttcgtcgc ggtgcgggat gacaataaca
      181 atggcatacc ggatgatgcg cgagtggcgg ttaatgatcg gacgtttggt tcaggaaaaa
      241 gttctgatac tacgtggaca ccaatgaaaa aacttctggc aaccaacgga atcctctccg
      301 gtgtcgatga acgctggggc ggccgcgtca atcctccgga cggtcgagat ggttacccgc
      361 ttaccaacgc aagaagtgac cgaccaaaga aaacgccaat tcttgacggc aaactctccc
      421 ccggcgaatg ggaagagttc gccacgttga acgaccgcca gttgcgggcc aagatctact
      481 tccaatggga tgactcagcg ctctactttg cggcggaaat gtcgcgcccg gccagtttgc
      541 acctcgatat cgatgccggg aacaacggct ggttctacgg cggtgacaac taccggcttg
      601 ccgtgaattc gaaagagacc ggcgctacgg actcagttgc gatttacgac tggagtttct
      661 ttccgcgtca gccgggattc tggaactacg agaaaattag gccggaagaa ttgcgctcgg
      721 cctgtacgat gggaaagagg aagtgcgtga tcgaattagc gattccgaag aatgaaggca
      781 cggggttgcg gctgcaggcg gggaatgaaa tgaatatcag gattggcttt gattggccag
      841 ggaacaagga aggaatggtc acgctctttg agccgcaccg gttttttggg gtgaagctgg
      901 ttgagaataa tgttcgatag tttggttgct gaagattcca aggaaaaaaa taaagagagg
      961 cgcctgcctc cctttatact aagaattttg actttttaga aaatccccaa ctctaatttt
     1021 gcctcttcgg agcacatggt cttggggtcc cagggcggct cccagaccag ttcgaaatag
     1081 acgtagcgca cgcccggcat actgccggat ctctcctcca cctgttcctt tatcacttcc
     1141 cccaatgggc aggcggggga agtaagggtg gcggtaacgt agacatcatg gtcgcgcact
     1201 tcgatgccgt aaatcaggcc caactccacg atggaaatgc caatttcggg gtcgacgatt
     1261 tccttgatca cctcgcggat ttgttcgggt gttggcggag gcaaggaggc gagggataaa
     1321 ggagcctggg cctcgggcgg gggggaagtc gccggtggag attgaggctg ggtcacatcg
     1381 ctattttcga ttttgtcctg aggaatctcg tcgctcattt caccttcacc cattcagatc
     1441 gaatgcgccg aggttcgtaa cctcggctac gaaaaacagg tccacgctca ttccattatc
     1501 acctgttgag ccggtttgtg ttctttcaga gcctttatac cctccagaag ggtgttccag
     1561 gccaaaagcg cgcacttgat ccgcaccggg tattttttga cgccctccaa cgcctcaagg
     1621 tcgccgaaat cttcgggcat cggctgcgac ccgttcccta acatcatttc cttgaactcg
     1681 cccgccagtt tttctgcatc cgcgagactc tgacctttga ttgcctcggt catcatcgag
     1741 gccgcggcct ggctgatgga acagccgtgg gtttcgatgc gcacgtcggc cagttgcccg
     1801 tccttttcca gaatgaaaag ttccacttca tccccgcaga gaggattggc gccctcgacc
     1861 tttaaggccg gttcgggcag tttcccccgg ttgcgcgggc ttttgaagtg gtctaagatg
     1921 atctcgcggt agagatcgtc acgtaccgac accggaaaaa atctcctgcg ccttgtggag
     1981 gccacggacg agcgcatcca cttcggctct ggtattgtag acgtggaaac tggcccgggc
     2041 agtgccggtg acgcctaact tgcgcatcaa gggctgacag cagtggtgcc cggcccggat
     2101 ggcgatgccc tcttcgttca ggatggtgcc caggtcgtgc gggtgcatct ggggcagatt
     2161 gaacgccacg acgccgcctc tggcttcggc tttcgacggg ccgtagattt tgatgtcgtc
     2221 aatttcgcgc aggcgtttga gtgtgtagtc gactaaatta atttcatgct ggcggatgtc
     2281 gtccattcca attttttcca gatagtcaat cgctgcacca aacgcaatag cgtcggcaat
     2341 gttcggcgtg ccggcttcaa acttccaggg gaggtagttg taagtggcgc ggtcgagcca
     2401 gacctggaga atcatatcgc caccgcccag gaaagggtcc attttctcca aaatttcctg
     2461 gcggccgtag aggatgccga tgccggtggg acctaacatc ttgtgggcgg agaaagccag
     2521 aaagtcgcaa tcaaatccgc ctgaggcgac cgcctgcacg tcggcgccga gatgcggcac
     2581 gccctgggcg ccgtcgatca tgaccagcgc acctttgcgg tgggcaaggt cggcaatctc
     2641 acgcacagga ttgatcgtcc ccaaaacgtt cgacatctgg gtgacgctga cgagtttagt
     2701 gcacggagtg aaaagggatt cgatatgatc gaggtcgagc aagccatcgt ccgtaacaga
     2761 aatgaatttc aatttcgcgc tggttgactg agccagcatt tgccagggga cgaggttgga
     2821 atggtgctcc atttcgctta agatgatttc atcaccagcc gcaagatttt ttcggcccca
     2881 ggagtaggcg acaagattaa tcgcttcggt ggcgttgcgg gtgaagatga ccgaggagga
     2941 agaaggcgcg ttgataaact tcgcaacttt cgcccgcgcc tgttcgactg cgtcggtggc
     3001 ttcttcgctt aaggtgtgaa tgccgcggtg gatattggca ttgtagcgtt cgtagtaatc
     3061 gaccagagca tcgatcacca cctgcggctt ctgggtggtg gcggcgttat cgagatagac
     3121 caggggcttc ccgttcac
//
LOCUS       DBNGZD010000352         5863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_334427, whole genome shotgun sequence.
ACCESSION   DBNGZD010000352 DBNGZD010000000
VERSION     DBNGZD010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_334427"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC16GM_09325"
     CDS             <1..660
                     /locus_tag="AC16GM_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923907.1"
                     /translation="DDRKRQGIYRGFGFENGGELRLKTGWVFRLLFGGNYSEGDSATS
                     VVQQATRTYLSDSLDIYVFRSRVNGTHIVRSGLVGVGMQKEVTASTRVLAGVRFQVLA
                     QRLDFHYTFFDSVSRFRNDTLIFQNGSESKRQFFSKPWSDLLTVPLALETNLSPNVEA
                     RFGTVWIHGFDRSQAALTTSLQYTLGLGFHFGDLSIDALASNSLTASLQQWRVSFEYR
                     L"
     gene            complement(662..1333)
                     /locus_tag="AC16GM_09330"
     CDS             complement(662..1333)
                     /locus_tag="AC16GM_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2923908.1"
                     /translation="MCSIAPLRESNGLIFEPLVVGPLEVNCYILAERPSGPAVVIDPG
                     ADSERILAVINRHHFYVEKIVLTHAHGDHIGAVSELKRKTGAKILIHPGDAEMLTSAE
                     KNLSLWAGLTVTAPAADQFLNEGDEITIGAVTLKVLHTPGHSPGGISLVGPDFVFTGD
                     LLFAGSVGRTDFPGADAPTLMRSIKEKILPLGDHFTIYPGHGDSSTIGEEKRTNFFLN
                     QRYFV"
     gene            complement(1336..3234)
                     /gene="uvrC"
                     /locus_tag="AC16GM_09335"
     CDS             complement(1336..3234)
                     /gene="uvrC"
                     /locus_tag="AC16GM_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR2923909.1"
                     /translation="MLEAVKAMERFSPALKAKLVNLPDQPGVYLMKDAAGKILYVGKA
                     KVLKHRVRSYFQSASGGPWDPKVQALVSKIADFEILATDNEMEALILEANLIKEHKPR
                     YNVNLKDDKRYPYLKLTSEPFPRLLVVRRVKKDKARYFGPYTDVKNMRKTLKLIGRIF
                     QIRSCNLPLPPPPGKKFRVCLEYFIKRCPGPCESKISQAAYQEMIDNAALFLEGKNVQ
                     LIKSLREKMEKYAAAEKFEQAALVRDQLLALESVVQKQKVVSQDEKDRDILAFARSSK
                     DICAVALQVRDGRLLSRQQYYLTGDKSDTDAEILSTFVKQYYVSQSLIPDEIYLPASL
                     EEGDLVRAWLEKKKGAKVAFVYPQRGEKAKLLEMAEQNAKLSLDELLLQKHRAEKKVP
                     EAVTLLKKDLFLDVLPRRIVAFDISNLGTSDPVGSAVYFEDARPKKSEYRHFQIKTVV
                     DQQDDFAMMREVVNRYFSRLIEEQRAFPDLVLIDGGKGQLSSALEALTDLNVTSQPII
                     GLAKRLEEVILSGAPESLTIPKSSPSLRLLQQIRDEAHRFAITYHRKKRTQRTIKSEL
                     DDIKGIGPARRQALLSQFGSVEQIKAADLEALKQVPGLGERAAQAVHEFFHPSVPVVA
                     EQPIISKA"
     gene            complement(3311..3766)
                     /locus_tag="AC16GM_09340"
     CDS             complement(3311..3766)
                     /locus_tag="AC16GM_09340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR2923910.1"
                     /translation="MDLEPSVRSGPTVQTAQAIQTLPEAGNGIDPARLDSIISRYLGT
                     PYGQRQGRSRFDCSGFIREVFRNYSGRDLPASSRDMFKRLEPIASDQALCGDLVFFGN
                     NGAVSHAGIYRGDGTFAHASKSLGVTISSFAEPYYRQRYLGARRVPLSR"
     gene            complement(3942..4859)
                     /locus_tag="AC16GM_09345"
     CDS             complement(3942..4859)
                     /locus_tag="AC16GM_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR2923911.1"
                     /translation="MLRFWDYFFVLRPVLWLPVWTVSLLGARSALRHQAGTTLGTRSN
                     LLLILTGALLFGAVYLLNQIFDIESDRVNRKLRFLPEKLISVTAAVRETVLLNMAALI
                     VAFSVSIQTGWLAASIVILGILYSAPPFRLKNHPLGGLLANMLAHGSIIFWLGWSFIG
                     KLSGTAVLQSLPYFLSVGGIYLNTTVPDIPGDARAGKRTLAVIWGPKWTRLLAVLLVT
                     TAALAAYRVHEVPLLVAALASLPPFLLALLQNSTRLTILSTKLALVVLSAFACYYSPP
                     YLLILIVGFLATRLYYRRRFGLVYPSLGT"
     gene            complement(4864..5691)
                     /locus_tag="AC16GM_09350"
     CDS             complement(4864..5691)
                     /locus_tag="AC16GM_09350"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR2923912.1"
                     /translation="MKNLAIRVAVATVAIPVILFICWRGGLWLFGFVLAISTLGLYEY
                     VRLIAPRYNLQRGWTFLLFSVGIPLVVHWYGEPALTPAVLVTFAILSLALVGTGQPVN
                     AVLGLGVAFLGIVYVPLLSSFLLLVHSLPDGMAGSSAFGGKWLILLFVTIWSCDTAAY
                     FVGKRLGKHKLAPVISPNKTIEGAVGGIIGGVAAAYLCQGTFFREALPKDALVVGMVV
                     GVIGQLGDLVESMLKRDAQVKDSSAIIPGHGGVLDRFDSLLFTLPCIYYYLKYFVYR"
     gene            complement(5741..>5863)
                     /locus_tag="AC16GM_09355"
     CDS             complement(5741..>5863)
                     /locus_tag="AC16GM_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HIR2923913.1"
                     /translation="QTSYTELYVTDVLWPDFSAEDFYRAIQEYQHRQRRFGGVA"
BASE COUNT         1363 a         1590 c         1701 g         1209 t
ORIGIN      
        1 gatgaccgaa agcgccaagg tatataccga gggtttggat tcgaaaacgg gggtgaattg
       61 agattaaaaa ctggttgggt attccgtctg ctgtttggtg gcaattacag tgaaggtgac
      121 tcagccacct ccgtggttca acaggccacc cgaacatatt tgtcagattc tcttgacatc
      181 tatgttttta gatctcgtgt aaacggaacg cacattgttc gatccggctt ggtaggggtg
      241 ggaatgcaga aggaagttac tgcttcgacg agagtcctgg ctggcgtgcg ttttcaagtc
      301 ttggcacaac ggcttgactt tcactataca tttttcgatt ccgtttcgcg cttcagaaac
      361 gatactctta tcttccagaa tggcagtgaa tccaaacgtc aatttttctc taagccgtgg
      421 tccgatctcc tgacggttcc tctggcgctc gaaaccaatc tatcacccaa tgttgaagca
      481 cgttttggta ctgtttggat tcacggtttt gatcgctcac aggccgcatt aactacctct
      541 ctccagtaca ctcttgggct gggttttcat tttggggact taagtatcga tgctctggca
      601 agcaatagtc tcactgcatc tttgcaacaa tggcgcgtaa gttttgagta ccgcctttag
      661 gtcacacaaa gtatctctga tttaaaaaga aattcgtacg cttctcttct ccgatggtgg
      721 aggagtcccc gtgccccggg tagatggtga agtgatcacc taagggcaga atcttctcct
      781 ttatcgagcg catcagagtc ggcgcgtcgg cgccggggaa atcagtccgc cccaccgaac
      841 cggcgaagag caaatccccc gtaaagacaa agtccggccc cacgagcgaa atgccgcccg
      901 gcgaatggcc cggcgtgtgg agcactttta aggtcaccgc cccgatggtg atttcatcgc
      961 cttcattcaa aaattgatcg gcggctggcg cggttaccgt cagaccggcc cagagggaga
     1021 gatttttctc ggcgctagtg agcatctcgg cgtcgccggg atgaatcaaa atcttggcgc
     1081 cggtttttct tttcaattcc gataccgcgc cgatgtggtc gccgtgggcg tgagtcaaaa
     1141 cgattttctc cacatagaag tggtggcggt tgatgacggc gagaattctt tcggagtccg
     1201 cgccggggtc gatcaccacc gccgggccag agggccgctc ggctaaaatg taacagttaa
     1261 cttctaaggg cccgaccacc agcggttcaa aaatcaaacc attcgattca cgcagcgggg
     1321 ctatactgca catacttacg cttttgagat tatcggttgc tcagccacta ccgggactga
     1381 gggatggaaa aactcatgca ccgcctgtgc cgcccgctcg cccaaaccgg gcacctgttt
     1441 gagcgcctcc aggtcagcgg ctttgatttg ctccaccgag ccaaattggg aaaggagcgc
     1501 ctgacgccga gcggggccga tgcctttgat gtcgtccagt tcggatttaa tcgtccgttg
     1561 cgtccgcttc ttgcggtgat acgtaatggc aaagcggtgg gcctcatccc ggatctgctg
     1621 aagaagtctg agagagggcg aggacttcgg aatggtgagg gattccgggg cgccggagag
     1681 aatcacttcc tccagccgct tggctaagcc gattatcggc tggctggtga cgttcagatc
     1741 ggtcagggct tcgagcgcac ttgagagttg tcccttcccg ccatcaatta aaaccaaatc
     1801 ggggaaagcc cgctgttcct caatcaaccg gctgaagtag cggttcacca cctcgcgcat
     1861 catggcaaaa tcgtcctgct ggtccacaac ggttttaatc tggaagtgcc ggtactccga
     1921 ctttttgggc cgggcgtcct cgaaataaac ggcagagccg accgggtcgc tcgtgccgag
     1981 gttggagatg tcgaaggcga cgatgcggcg cgggaggaca tccagaaaga ggtctttctt
     2041 caagagggtc accgcctccg gaactttttt ctcggcccgg tgcttctgga ggaggagttc
     2101 gtctaatgaa agtttcgcat tctgctcggc catctccaac aacttggcct tctcgccccg
     2161 ctgggggtag acgaaggcaa ctttggcccc tttctttttt tccagccagg cgcgcaccaa
     2221 atctccctcc tcgagcgaag ccggcaggta aatctcgtcc ggaatgagcg actggctgac
     2281 gtagtattgc ttcacgaatg tggagagaat ttcagcgtcg gtgtcggatt tgtcgccggt
     2341 gagatagtac tgctggcggc ttaagaggcg gccgtcgcgc acctggagcg ccaccgcgca
     2401 aatgtccttg ctggagcggg caaaggccag gatgtcgcga tccttttcat cctgactcac
     2461 caccttttgt ttctggacga cgctttccag tgcgagaagt tgatcgcgca ccagcgcggc
     2521 ctgttcaaac ttctccgccg cggcatactt ttccattttc tcgcgcagac ttttgatcaa
     2581 ctggacattt ttgccctcga ggaacagcgc agcgttgtca atcatttcct gatacgctgc
     2641 ctgagaaatc ttggactcgc aggggccggg gcaacgcttg atgaaatatt ccaggcaaac
     2701 ccggaatttc ttgccgggcg gcgggggtaa ggggaggttg cacgaccgaa tctggaaaat
     2761 ccggccgatc aatttcaacg tcttgcgcat atttttgacg tcggtgtagg ggccgaagta
     2821 gcgcgcttta tccttcttca cccggcggac gactaagagg cgggggaacg gctcagaggt
     2881 cagttttaaa tagggatagc gcttgtcatc cttcaagttc acgttgtagc gcggtttgtg
     2941 ctctttgata aggttcgcct caaggataag cgcttccatt tcgttgtcgg tggctaagat
     3001 ttcaaagtcg gcaattttgg agaccagcgc ctgcactttc ggatcccagg gtccgcctga
     3061 ggcggactgg aagtaggaac gcacccggtg cttgaggacc ttcgccttcc cgacgtagag
     3121 gatttttccc gccgcatctt tcatcaggta aaccccgggc tggtcgggaa gattcaccag
     3181 tttggctttg agcgccggac tgaaacgctc catcgctttt accgcttcga gcatgcctat
     3241 aaacttacgc tctgcagcag aaaaaagcga gagcgaggag cggctccggg aaaaaggtgg
     3301 tggcacgaga tcaccgcgag agcggcaccc ggcgggcgcc taagtaccgc tgccggtagt
     3361 agggctcggc aaacgaagaa atcgtcaccc ccagtgactt cgacgcgtgg gcgaaggtgc
     3421 catctccccg gtaaattccg gcgtggctga ccgcaccgtt attcccaaag aagaccagat
     3481 cgccgcagag cgcctgatcg gaggcaatcg gttccagtcg cttgaacata tcacgactgg
     3541 aggcggggag gtcccggccg gaataattgc ggaatacttc ccggatgaaa ccggagcagt
     3601 cgaaccggct ccgcccctga cgctggccat agggggtgcc caggtaacgc gatatgatgc
     3661 tgtccagccg ggccgggtcg atgccgtttc cggcctccgg gagcgtctgt atagcctgtg
     3721 ccgtttggac cgttggccca gaccggaccg aaggctccag gtccacgtct gaccagggcc
     3781 gagacgtata ccgcggagcc ggtacacaac caccggctaa aacaagcagc agaatgccga
     3841 gaaggacagg gcgcattttt gctcctgaaa tgaccttctt cgtttatcgg aaaagccaac
     3901 ctgaaaattg agatgaaatt actgcgggag tccgattgga atcaggtccc caaagagggg
     3961 tacaccagac cgaaccggcg ccggtagtag agccgggttg ccaaaaagcc tacgatcaga
     4021 atcaagaggt acggggggga gtaataacag gcgaaggcgg aaaggactac taaagccaat
     4081 ttggtggaga ggattgtcaa gcgcgtgctg ttttgcaaga gcgccagaag gaaaggcggc
     4141 agggaagcca gagcggcgac caaaagcggc acttcgtgta cgcggtacgc cgccagggcc
     4201 gcagtcgtaa cgagtaagac ggcgagcagc cgagtccatt tcggccccca gataaccgcc
     4261 aaagtgcgct tcccggcgcg ggcgtcaccc gggatatcgg gaacggtagt attcaaataa
     4321 atcccgccca cggacaaaaa gtaggggagc gattggagca ccgccgtccc gctcagcttc
     4381 ccgataaaac tccaacccaa ccagaagatg atgctgccat gggcaagcat attcgccaga
     4441 agaccgccca ggggatggtt cttaagccgg aagggaggcg ccgagtagag gatccctaaa
     4501 atgacaatgc ttgcggccag ccaacccgtc tgaatggaaa cagaaaaggc gacgatcaaa
     4561 gccgccatgt tgagcagtac cgtttcacgt acagcggcag taaccgaaat taatttctcc
     4621 ggcagaaaac ggagtttgcg gttgacccgg tcggattcga tgtcgaaaat ctgattcagc
     4681 aggtagacgg caccgaagag gagcgcaccg gtcaggatca aaagcagatt cgaccttgtt
     4741 cccaaggttg tcccggcctg atggcggagc gcgctccggg cgcccagaag tgaaactgtc
     4801 cagaccggca gccagagaac gggccgcagg acgaaaaagt aatcccaaaa acgtagcatc
     4861 accctaacgg tagacgaagt acttcaggta gtagtaaatg cagggcaagg tgaagagcag
     4921 actgtcgaaa cggtcgagga cgccgccgtg accggggata atggccgaag aatctttcac
     4981 ctgtgcgtcg cgcttgagca tcgactcaac cagatcacct aactggccga tgacgccgac
     5041 caccattccc accacgagcg cgtctttcgg cagggcttcg cggaagaacg taccctggca
     5101 gaggtaggcg gcggcaacgc cgccgatgat accgccgacc gcgccttcga tggttttgtt
     5161 cgggctgatt accggagcca gtttatgttt ccctaacctc ttgcccacga aataggcggc
     5221 ggtatcgcaa ctccagatcg tcacaaagag gaggatgagc cacttgccgc cgaaggcgga
     5281 actccccgcc atcccatccg gcagactgtg gactaaaaga aggaaactcg acagaagggg
     5341 cacgtaaacg attcctagaa aagcgacgcc cagccccaag accgcgttca ccggctggcc
     5401 ggtgccgacc agcgccagac tcaaaatggc aaacgtcacc agcaccgccg gagtcagcgc
     5461 cggttcgcca taccagtgca ccaccagggg gataccgacc gaaaagagca aaaaagtcca
     5521 gccgcgctgg aggttgtagc gcggggcaat caggcggacg tactcgtaca agcctaaagt
     5581 cgagatggcg agtacgaagc cgaagagcca aagtccgccg cgccagcaaa tgaaaagaat
     5641 caccgggatg gcaacggtgg cgacggccac acggatggcg agatttttca taagcggcaa
     5701 gcggatgggt tggaatcgtt gcgggaagag agcggttcca ttacgccact cccccaaagc
     5761 gcctctgtcg gtgctggtac tcctgaattg cgcggtagaa atcttccgcg ctaaaatcgg
     5821 gccagagaac gtcggtaacg tacagttcgg tataagaagt ttg
//
LOCUS       DBNGZD010000353         2943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_750423, whole genome shotgun sequence.
ACCESSION   DBNGZD010000353 DBNGZD010000000
VERSION     DBNGZD010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_750423"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            523..1920
                     /gene="purF"
                     /locus_tag="AC16GM_09360"
     CDS             523..1920
                     /gene="purF"
                     /locus_tag="AC16GM_09360"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR2923914.1"
                     /translation="MENSLTEKCGVFGVYGARNAAELIYFGLYSLQHRGQECAGIVTV
                     NDGRLHLRKGMGLVNDLFPSRDFLSDLKGSFGIGHNRYSTTGSSNEINIQPLLMAYRD
                     RQLAVGHNGNLTNSISLKASLEKSGAIFQTTSDTELILHLVARSKKGKPVQKICEALT
                     KVQGAYSLVFATNDSIIAARDPMGFRPLALGRLGRAHVVASETCAFDLIGAKYERDVE
                     PGEVLEISAKGLKSYQPFKSQPHAFCIFEFIYFARPDSMIFGENVDKVRRRLGRQLAI
                     EHPVEADIVTSVPDSSNTAALGFSEQSKIRYEIGFIRNHYVGRTFIQPSQEIRDLDVK
                     VKFNPVKGVLNDKRVVIVDDSIVRGTTSKKLVAMVREAGAKEVHFRVSCPPIVSPCFY
                     GIDMPTKRELIGSYKTVEEIRRFLGVDSLGYLSIESMLSMSSLPRGETFCTACFSGKY
                     PTKIENNIGKLSLEN"
     gene            complement(1970..>2943)
                     /locus_tag="AC16GM_09365"
     CDS             complement(1970..>2943)
                     /locus_tag="AC16GM_09365"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HIR2923915.1"
                     /translation="LLVNDALADERFGGSEWNGMPIRSVLAVPLKVKNQLIGFLALFN
                     KKEGAFTPEDQRLLSIVGMQAAQTIEAARLYAEEKKVAALEEDLRTAKIIQQNLLPKS
                     TPQIPGFDVAGMTVPAKEVGGDYFDYLPLPDGRWGFVVADVAGKGLPAAMLMSNLQAT
                     IRGQSAVSASCAQCIATANRMLMLSVGTGRFVTVFLAALDGARKMLSYSNGGHNRPYL
                     FKPDGAFRELATGGPLLGVVDSLPYEEEAVPLAPGDVLVIYSDGVTEAFNEKGEQFGE
                     KRLVETLKPVLSYSAAEILAAVTNAVHSFAGNTPAADDLTLLVVKAV"
BASE COUNT          614 a          853 c          835 g          641 t
ORIGIN      
        1 accgcctgcg ccagggcctc ccgggcgatc tctccgatcg tcgccggcga gttgatcgct
       61 aaggcgatgt tccgcagggt caggaacttg cggtagttct gctcggccgg tggctccata
      121 aactcaaata gcggctctct tctcattcct gttcccgcaa gccctgtgcc ttgaccgggt
      181 cacctgggct ctgatttgtg gccgtcccgg ctccccgaca gttaaagttt cagataaaca
      241 gagtcatata acatattgat aaaacagtgc ttaaaaaaac attgctctgc agaccgccct
      301 cccctcaaat cagccgctgt ggggaactta caaaaaaaga gcaccccggg caatgacttg
      361 gaacgacttt tcaccttccc tgcaaaagac cgaaattcat cggcttgaca gggactccat
      421 cgcccgtaga ttcctgccgt ataaacttac ccacagaggg tgggaagcca gtcgagtgga
      481 gcctgatcct taagccgatc tgaagggtgt cgccgagcgg tgattgaaaa ttccttgacc
      541 gaaaagtgcg gcgtctttgg cgtctacggc gcccggaatg cggccgagtt gatctacttc
      601 ggcctctact cccttcagca ccggggccag gagtgcgcgg ggattgtcac cgtcaacgac
      661 gggcgtttgc acctgcgtaa ggggatgggt ctggtcaacg acctcttccc ctcgcgcgat
      721 ttcctctccg acctcaaagg ttccttcggc atcgggcaca accgctactc caccaccggc
      781 tcctccaatg agattaacat ccagccgctt ttgatggcct accgggaccg ccagttggcg
      841 gtggggcaca atgggaacct gaccaattcg atttctttga aagcgtcgct ggagaaatcg
      901 ggcgccattt tccagaccac gtcggatacc gaactgattc tgcacttagt cgcccgctca
      961 aagaaaggaa aaccggtaca gaagatttgc gaggcgctta cgaaagtcca gggcgcttat
     1021 tcgctcgtct ttgccacgaa cgattcgatc atcgctgccc gcgacccgat gggcttccgt
     1081 ccgctggcct tgggacggct gggccgggca cacgtcgtcg cctccgagac ctgcgccttt
     1141 gacctgattg gcgcgaagta tgaacgggac gtggagccgg gtgaggtgtt agagatttcg
     1201 gctaaggggt tgaaatcgta ccaaccgttc aagtctcaac cgcacgcctt ttgcattttt
     1261 gagttcatct actttgcccg gcccgactcg atgattttcg gcgaaaacgt cgataaagtc
     1321 cgccgccggc tgggccgcca gttggccatc gagcacccgg tggaagccga catcgtgacc
     1381 tcggtgcccg actcctccaa caccgccgct ctgggctttt ccgagcagtc gaaaatccgc
     1441 tacgaaatcg gcttcatccg caaccactac gtggggcgga ctttcatcca gccgtcgcag
     1501 gagattcgtg acttagatgt gaaggtgaag ttcaacccgg ttaagggggt cttaaacgac
     1561 aagcgggtgg taattgtgga cgattccatc gtgcgcggga cgacctccaa aaaacttgtg
     1621 gcaatggtgc gggaggcagg ggcgaaggaa gtccacttcc gcgtctcctg cccgccgatt
     1681 gtctccccct gcttctacgg gattgatatg ccgaccaagc gggagttgat tggttcatac
     1741 aagacggtgg aggagattcg caggtttctc ggtgtcgatt ccctgggcta cctctcgatt
     1801 gaaagcatgc tctcgatgtc gtctctgccc cggggcgaga ctttctgcac cgcctgcttt
     1861 tcagggaagt atccgaccaa gattgagaac aatatcggga aattatcgct ggaaaactaa
     1921 atttgctgcg ttagaagtta ctgccagcat agtattaaaa gtcttttttt cacaccgcct
     1981 tcaccaccag cagggtcaaa tcatccgcgg cgggggtgtt gccggcaaag gagtggacgg
     2041 cgttggtaac ggcggcaaga atttcagccg ccgaatagga aagcaccggc tttagtgttt
     2101 ccaccagccg cttttccccg aattgttctc ctttttcatt gaacgcttcc gtaacgccat
     2161 cggaatagat taccagcacg tcgcccggcg caagcgggac ggcttcttcc tcatagggca
     2221 gggaatcaac cacccccaaa agcggcccgc cggtggcgag ttcgcgaaat gctccatccg
     2281 gcttaaagag atagggccga ttgtgcccgc cgttggagta ggagagcatt ttgcgggccc
     2341 cgtctaaagc agctaagaag acggtgacaa aacgccccgt tccgaccgag agcatgagca
     2401 ttcggttggc agtggcaata cactgggcgc aggaggccga aaccgctgac tgcccccgga
     2461 tggtggcctg caggttcgac atgagcatgg cggcgggcag gcccttgccg gccacgtcgg
     2521 caacgacgaa gccccagcgc ccgtccggga gggggaggta atcgaagtag tcgccgccca
     2581 cttctttggc cggcaccgtc attccggcca cgtcaaagcc ggggatttgg ggcgtggact
     2641 tgggcaaaag attctgctgg atgatcttgg ccgtgcgcag gtcttcttcc aacgccgcaa
     2701 cttttttctc ttctgcgtac agccgcgccg cttcgatggt ctgcgccgcc tgcatcccga
     2761 cgatggagag caagcgctgg tcttccgggg tgaacgctcc ctcttttttg ttgaagagcg
     2821 ccagaaaccc gatgagttga tttttcactt tcagcgggac ggctaagact gagcggatcg
     2881 gcatgccgtt ccattcgctc ccgccaaagc gctcgtcggc caaggcgtcg ttgactaaaa
     2941 gcg
//
LOCUS       DBNGZD010000354         1126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_125514, whole genome shotgun sequence.
ACCESSION   DBNGZD010000354 DBNGZD010000000
VERSION     DBNGZD010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_125514"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..>1126
                     /locus_tag="AC16GM_09370"
     CDS             64..>1126
                     /locus_tag="AC16GM_09370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR2923916.1"
                     /translation="MPAFLSGASSVQFVWSGAVTPTSAVVKAKLITEGGIARLAVSTA
                     PDLTSLTYSNWDTAVTAENNRVVRLEVNGLAPRQRYYYAVEVDGALDTEKVGRFQTFP
                     TDTGTFTFALGSCAQTGSNSLAFATIRSLDPLFFFHLGDFHYQNIGVNDPNLFRQAYE
                     TVLSSPNQSRLYQDFALAYIWDDHDYGPNNSDSTSPSRAASRATYREYVPHYPLAAGG
                     GNVPIYFTFDVGRVHFIVCDSRSARSPEFAVDNASKTMLGTEQKAWFKQELLNSKSNY
                     PLLVWVNTLPWIGSPGGGDAWYEYTNERRELADFLEVNGIKNICMLSGDAHMLAIDDG
                     ANSDYATGGGAGFPVFHAAS"
BASE COUNT          212 a          327 c          325 g          262 t
ORIGIN      
        1 attggttgcg ggactaaaag atcgagagtt ttcctttcac ttttcccgat ggtcctcctt
       61 tttatgcctg ccttcttgtc cggcgcctca tcggtgcagt ttgtctggtc gggggcggtg
      121 acgcccacat cggctgtggt gaaagcaaaa ctgattacgg aggggggcat cgcccgattg
      181 gccgtgagca cggcaccgga tttaacctct ctcacctatt ccaactggga cacggcggtg
      241 acggctgaga acaaccgggt ggtgcgattg gaagtgaatg ggctggctcc ccggcaaagg
      301 tactactatg ccgtggaagt ggacggcgcg ctcgacactg aaaaagtggg gcggtttcaa
      361 acttttccga ccgataccgg cacctttacg ttcgctttgg gttcctgcgc ccagaccggc
      421 tccaacagtc tggcgtttgc aaccatccgt tcgcttgatc cgcttttctt ttttcatctt
      481 ggtgattttc actatcaaaa tatcggggtg aacgacccga atcttttccg gcaggcgtac
      541 gagaccgtgc tttcctcccc caatcagtcc cggctgtatc aggatttcgc cctggcctac
      601 atttgggacg accacgacta cggcccgaac aattcggatt cgacttcgcc aagccgggcc
      661 gcctcccggg cgacctaccg ggaatacgtg ccgcattatc cgctcgcggc cggaggcggc
      721 aacgtgccga tttactttac ctttgacgtg ggacgggtgc atttcatcgt ctgcgattcc
      781 cgctcggccc gcagcccgga gtttgctgtc gataacgcat caaagacgat gctggggacg
      841 gagcagaagg cctggttcaa gcaggagctg ttgaattcaa aatcaaacta cccgctgctc
      901 gtctgggtga acacgcttcc ctggattggc tcccccggcg gcggggatgc ctggtatgaa
      961 tacaccaacg agcgccggga gctggccgat tttctcgaag tgaatgggat taagaatatc
     1021 tgcatgcttt ccggcgacgc ccatatgctg gcgattgacg acggcgccaa cagcgactac
     1081 gccaccggcg gcggggcggg attcccggtc tttcatgccg cctcgc
//
LOCUS       DBNGZD010000355        11621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_543529, whole genome shotgun sequence.
ACCESSION   DBNGZD010000355 DBNGZD010000000
VERSION     DBNGZD010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_543529"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="AC16GM_09375"
     CDS             <1..1087
                     /locus_tag="AC16GM_09375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2923917.1"
                     /translation="SETAKLTPDDPELLPLVGPQSYPPKNDFHKKTADFSPEERAKSV
                     KPIFEQCKKEGLTAAGYLSNGSGVNAFATSKGCFAYYTATDAGFSTTITSPDSTGWAL
                     DSSPNIAELDIGKDAGIAIQKAKASRNPKEVPPGDYTVILEPAAVTDLLVFLMFGFSA
                     RQVMEKQSFLTDKVGTKLFGENITLLDDCYHPLQSGQPFDGEGVPRQRLVLVEKGVVK
                     NLCYDRLTAKKEGKEPTGHGFSVPNTFGAMPGNLVMSGDSTTVDDMIKSIDKGILVTR
                     FWYNRLVEPMKVIVTGMTRDGTFYIENGKIQYGIKNMRFDESVVEMLNSVQALGKPVR
                     ASGVESPPMVVPPLKVAGFHFQSTTKF"
     gene            1300..1977
                     /locus_tag="AC16GM_09380"
     CDS             1300..1977
                     /locus_tag="AC16GM_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2923918.1"
                     /translation="MAQSKLSIHQATLDEPNQATPEVTTEEVLKILTSGIEPLLDVRS
                     AKEYAIAHIPGSINLYEKEAERIMQLYPDKAARLVLYCNGPYCGKSKRTTEQLVKLGY
                     TNVRRYQLGLPVWRALGHTVQTDLEGFRYVFMNDKTAVFVDARRPAQFADGSLPGAVS
                     IQAGEAEQANEDGRLPHTDKGTRVIVFANSPQEARTVAEEIAKKAYWNSSYFGGTIEE
                     LKRAGLW"
     gene            2002..2244
                     /locus_tag="AC16GM_09385"
     CDS             2002..2244
                     /locus_tag="AC16GM_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR2923919.1"
                     /translation="MVEFLLSRRDFLQLSAVSIALWALPLAGVASGESFFSLITPNAD
                     FLGNHGSRRRGCSIPTLSQPRSINPDLVVHFSDFRL"
     gene            2410..4224
                     /locus_tag="AC16GM_09390"
     CDS             2410..4224
                     /locus_tag="AC16GM_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2923920.1"
                     /translation="MPAETVTTRLSDEAIDKALVTAYRDLWGTFAKAVKAAVLYPPEN
                     PLPSQFRSQLTDKLTALLKAGDDLVFSFGPHLIRLGPQEVYSEPESKDSFVALFHRDG
                     VHQISFSPGVEAAETAGLIDAVAKALSSHGDGDDLVTLLWQKEFLHIKYQVVEDVAEV
                     AAVELNLGKGSKAESDPLVYVNLVLQEGKAGTGEGSGEGDKAGEGQAAAPPAHAAAVR
                     KVFGDIEQMAQEDRAAIARELSENKVFPWSKEALELLCEMVYLENEPAGVAETVGILE
                     KILDELIAGGRVQPTAQIVTSLRQLLEDFRSKPPEWARPVQAALDKAGDPIRLSRMTE
                     LLNANKVDDPEAVAHYLRTLDWKSVGNIHALLGTLEHYPHRKLVCQVLEQMGPPAVEL
                     IAKGMADPRWFVVRNTALILGHIGGDKACQHLRAGLSHSDVRVRRETLLALEQIGTPT
                     ASKMVLGVLNEKDGKLRSKALQILVHLQEAEACEPLWLRIESKDFLQWSPEEQRELVF
                     AYAQIGRDRVLEELAGLVTRQAFFKRGLYQPIKEFAVEAMAKVGTLRAQEYLERLAGT
                     THGALKTACQTWAQRLKRRQVQGVVPARTAASRGDEHE"
     gene            4217..5725
                     /locus_tag="AC16GM_09395"
     CDS             4217..5725
                     /locus_tag="AC16GM_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR2923921.1"
                     /translation="MSDPRSEFPSSAAVEPSHPDPRQRRDKSSDLSENMRRQLVVQMY
                     VIFRTAQLYEPANSAYQKALEALLHTLSECFAIEGEAQIVFRQGYWYVNGARTRFEID
                     GRTAAESLAQLFEQRQIGGMGFEPAVSREELVRFADLFLHTEAPTPEERLALLMKKLD
                     AAQVLTIILFSPNVSPAEGLADLLTLESSRRSARRTFFYAVTMVEGLMQRAQHGESVN
                     FLKAKRVVHSIIDHLLRDEGALVELATLKTYDDYTYAHSVNVSVLSLALGLRVGLDRH
                     NLSLLGFAALFHDVGKTRLPVELIHKPTEYDEMDWRQMEQHPILGAKAIALTRAFDEY
                     TARGMLVAFRHHTNQDLSGYPKLRQPRASDLFTRIVKIADSYNAMTSGRAYIKESNTP
                     DEAIRKMLLVSGKAYDSLLLKVFVQVLGIFPVGSLVLLDTQELALVFRANKSDLSRPQ
                     VRLIADATGLKEGLVVVDLAERESPEGPYRRSIVRVLDPKKYGLDLSRFALN"
     gene            complement(5980..6384)
                     /locus_tag="AC16GM_09400"
     CDS             complement(5980..6384)
                     /locus_tag="AC16GM_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR2923922.1"
                     /translation="MRKSFALLVLTAFTLAFVALPLVLAADKDLKTVTLSVKGMTCAG
                     CKKGVESALLKANGVEAASVDLETNTATVTYDGSATTVKRVMGSVKKAGFKAEMKNKE
                     ISETDCGPCPHDVKATTSTNGKACCTAKKQGT"
     gene            complement(6584..7084)
                     /locus_tag="AC16GM_09405"
     CDS             complement(6584..7084)
                     /locus_tag="AC16GM_09405"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR2923923.1"
                     /translation="MSGLRRHIVGIGVLSLATALFFGLAVAQEAGQKAPGFVLNNLAG
                     KPVTLADFAGKVVFLDFWATWCGPCIMELPHLKELYATYKDSGLVVIGVAFASGSAKD
                     VAKFAQKHGLTYPLLMGDDKIGKAYGGIVGFPTQFIIDRKGILVKKFFGYKNRSQIEE
                     EIKKLL"
     gene            complement(7206..7772)
                     /locus_tag="AC16GM_09410"
     CDS             complement(7206..7772)
                     /locus_tag="AC16GM_09410"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2923924.1"
                     /translation="MKRFCTVGVGVLALTLVLTGHLGAQKKAADKSVKKSAKPTEQNA
                     EVVWLTYEEGLKQAAKENKHVMVDFYTTWCGWCKKLDKDTYANPKVQTILRKHYVAVK
                     VNAESNRSFENEGKTMTEAQLASGKYGVTGYPTIWFLKPNGEAITPLPGYVGPDQFVH
                     IINYIKDDLYEKMEFDDYLKQQEGKSKS"
     gene            complement(7776..8849)
                     /locus_tag="AC16GM_09415"
     CDS             complement(7776..8849)
                     /locus_tag="AC16GM_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR2923925.1"
                     /translation="MIEHSVALFFKSLADLVVDSAFWLLGGFLLAGLIHEFIPAKTIA
                     RHLSGKGFGPVAKAALIGTPLPLCSCGVIPTAVGLRKDGASKPATVSFLISTPETGVD
                     SIAVSYALLDPFLTILRPVAAVLTSLAAGTVELLFGQKENRLPLSVTTLPGATTLRLS
                     WLKRFAQAIRYGFGDLLADIALYLVFGFLLSAALSALLPANWITRTLGRSDFTAMLLM
                     AGVSTPLYICSTSATPLASVLLLKGLSPGAALVFLLVGPATNTTTMVAVAKFLGKRSL
                     VLYLLSIVVTSLLLGLAVNAFYRGMGTPSMEKVAAGADIIPNGVAQFSGLVLAAILLG
                     HTGRATVKRFRSQRASGPSRRER"
     gene            9007..9405
                     /locus_tag="AC16GM_09420"
     CDS             9007..9405
                     /locus_tag="AC16GM_09420"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR2923926.1"
                     /translation="MEKFANLLKTLGEPIRLRILRLLLAAQRELCVCEFVDALEVPQY
                     NVSKHLHALRGAGLLTLRKEGRWVYFSLAQKTSFQKSLLKALSGLSDEILDQDRKELQ
                     KRLALRVKDKCLLGIQKKKLLVAGLGAQLE"
     gene            9402..10328
                     /locus_tag="AC16GM_09425"
     CDS             9402..10328
                     /locus_tag="AC16GM_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923927.1"
                     /translation="MKPFILLGLSGLLLHGTAMAAKPQMSGGVDLRYENRNGNKHSLR
                     VEGVFLNMRKVFSDQEGDSLITVGQIDWDDNFDRLRPYQTYAQYKGPLGKWNLSAGHF
                     ILPYGLLSDYDTERLLLQTQELKALGIKLDTGLKCSGRFRTVDYAFSVTQGVGRTRLE
                     DADGNKLVTGRIGWAGNDVSTGFSILNGKVLIDGDDRTLPVYERRAGFDLTKYSGPTT
                     VRGELTWGRNNGQTTYSGYFGMDHALSTKVEVNFKYAYWQGNATEHFLGVGFSYNLWA
                     RVILRAADEYQFSQRDQNAFSLQVYWDFLKIL"
     gene            10408..10866
                     /locus_tag="AC16GM_09430"
     CDS             10408..10866
                     /locus_tag="AC16GM_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923928.1"
                     /translation="MVNIFPQAKDYRTVDRKISEEKRAIIEKQLGEKLAPGEREDWTY
                     YEIMGKENGLLGFISADAEKGEFGVIEVVVGITPEGKVKGVYIQRAREKNKEFKSREF
                     LSQFEGKTVKDSLLIGKGIQVKERSLPVQMTVLGVRKMLIFYDQLANPRR"
     gene            10880..11299
                     /locus_tag="AC16GM_09435"
     CDS             10880..11299
                     /locus_tag="AC16GM_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923929.1"
                     /translation="MKKIGIISAVGLAVLLGAVSVTAKTKKMGKRIPEKATVVKLSDI
                     LKAPEKFKDKEVILQGTYVNYCCATDFVYREGLESVEIYPQGFLTPKLDKGKQMKVWG
                     IVRVREREEGEQQETRKNAKEEEHAEIIIEAEGLEVK"
     gene            11296..>11621
                     /locus_tag="AC16GM_09440"
     CDS             11296..>11621
                     /locus_tag="AC16GM_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2923930.1"
                     /translation="MTIFSLALKNLKRHRTRTILTILGIAVAAATLFSILSFDAGYKK
                     ALQEELAGSGIHLFVSTEGCPLEAASLIIHGGEIPRFLNMQTFNAVKDIPGVKQAGGF
                     LIFSLPS"
BASE COUNT         2696 a         3139 c         3312 g         2474 t
ORIGIN      
        1 gagcgaaacc gccaaactga cgccggatga tccggaactt ttgcctttgg tcggcccgca
       61 gagctatccg ccgaaaaacg acttccataa gaaaactgcc gacttttctc cggaggagag
      121 agcgaagtcg gttaaaccga tatttgaaca gtgcaaaaaa gagggcctga ccgctgccgg
      181 ctacctctcc aacggttccg gggtcaatgc cttcgccacc tcaaagggtt gcttcgctta
      241 ctacaccgct acggacgccg gattttcaac gaccattacc tctcccgatt ccaccggctg
      301 ggcgctggat tcgtctccta acattgcgga actggatatc gggaaggatg cggggattgc
      361 catccagaag gccaaggcca gccgcaatcc gaaagaagtg ccgcccggcg actacacggt
      421 gattttagag ccggccgccg tcactgatct tctggtcttt ctcatgttcg gtttttccgc
      481 ccggcaggta atggagaagc aatcctttct cactgacaaa gtcggcacca aacttttcgg
      541 cgagaacatc actctccttg acgactgtta ccatccgctt caatcgggcc agccgttcga
      601 cggcgaaggg gtcccgcgcc agcggctcgt tctggtcgaa aaaggagtgg tgaaaaacct
      661 ctgctacgac cggctgacgg caaaaaagga ggggaaggag ccaaccgggc acggtttttc
      721 ggtacccaat accttcggcg ctatgccggg gaacttagtg atgtcgggtg acagtacgac
      781 ggttgacgat atgatcaaat caatagacaa gggcatcctc gtcacccgct tctggtacaa
      841 ccggctggtg gagccgatga aagtaatcgt caccgggatg acccgggacg gtacgtttta
      901 catcgagaac gggaagattc agtacggaat caagaatatg cgcttcgacg aaagcgtggt
      961 cgagatgctg aacagcgtgc aagccctggg aaagccggtg cgggcctccg gcgtggagtc
     1021 gccgccgatg gtggtgccac ctttgaaagt ggccggtttc cacttccaga gcacgacgaa
     1081 gttctaatgc tgaccggaca attccgtctt gacgactcga ttctaaaaat catagatgaa
     1141 accacatttc ccaaaacgct gtaatgtatg ggtccaggtg ctcatgaatc aggtttttca
     1201 aaggcaggag cgacagaaat gacacattct cttttcgccc gagtcttgtc ggtgtgggca
     1261 ctagcggtag catgttttct gggaatatcg aattcaaccg tggcacagtc gaagctaagt
     1321 atccatcaag ccacacttga tgagcccaac caggcaacac cggaggttac gaccgaggaa
     1381 gtcctgaaaa ttcttaccag tgggattgaa cccttgcttg acgtgcggtc tgcgaaggag
     1441 tacgccattg cgcacattcc ggggagcatt aacctctatg agaaagaggc ggaacgtatc
     1501 atgcagcttt acccggacaa ggcagcccgc ctggttctct actgcaacgg tccgtattgc
     1561 ggaaagagca agcgaaccac cgagcaactg gtcaaactgg gttacaccaa cgtgaggcgc
     1621 tatcaattgg gccttccggt ctggcgggca ctcggccaca ccgtgcaaac ggacttggaa
     1681 ggttttcgat atgttttcat gaatgataag accgcggttt tcgtcgacgc gcgccgtccc
     1741 gctcagttcg cggacggcag tctgcctggt gccgttagca ttcaggctgg cgaggcagaa
     1801 caagcgaacg aagacggtcg cctcccccac acggataaag ggacgcgcgt gatcgttttt
     1861 gcgaacagcc cacaagaggc ccgcacggtc gctgaggaaa tcgcgaagaa agcatattgg
     1921 aacagcagct actttggagg aacgattgag gaattgaaac gtgcgggttt gtggtaaaca
     1981 taaaaacgca gcaagtaacc gatggtagaa ttccttctct cccgccgtga ttttttgcaa
     2041 ctgagcgcgg tcagcatcgc actctgggcg ctcccgttgg cgggagtggc ttccggtgaa
     2101 agctttttct ctctgatcac gccgaacgcc gactttttag gcaaccacgg ctcccggcgc
     2161 cggggctgtt caatcccaac cctcagccaa ccccggagca tcaatccaga tctggtagtt
     2221 cacttttcag atttccgact gtagggacgc tttagcttat tagtctctac cccccagagg
     2281 cccactcctg agccattctc cccgataacg ggttttccca aaggaaagag ccgctccccc
     2341 ggttcaatca gccggtctat cttcaagtca tttctgccga taacagaaaa ggactgaaag
     2401 aagagcatta tgccggctga aacggttaca acccgccttt ccgacgaggc aattgacaag
     2461 gcgttggtca ccgcgtaccg ggatctgtgg ggtacctttg ccaaggcggt gaaagcggcg
     2521 gtcctctacc ctcccgaaaa cccgctcccc agtcagttcc ggagccagct gaccgacaaa
     2581 ttgaccgctc tcctcaaggc aggagacgat ttagtctttt ccttcgggcc ccatctgatc
     2641 cgcctcggac cgcaggaggt ctattccgaa ccggaaagca aggatagctt cgtggccctc
     2701 tttcaccggg acggggtgca ccagatcagt ttttctccgg gagtggaggc ggctgagacg
     2761 gccggtttga ttgacgccgt cgccaaagcc ctttcctccc acggcgacgg tgacgacttg
     2821 gtcacgctgc tctggcagaa agaatttctc cacatcaagt accaggtagt tgaagacgtg
     2881 gccgaagtgg cggcggtgga attgaacctc ggcaaaggga gcaaagccga gtccgatcct
     2941 ttggtgtacg tgaacctggt gctgcaggaa gggaaagccg gcaccggaga gggttcgggt
     3001 gaaggggaca aagcgggcga aggtcaggca gcggcgccgc cggcccacgc cgccgcagtg
     3061 cgcaaagtct tcggcgacat tgaacagatg gcccaggaag accgcgccgc cattgcccgg
     3121 gaactgtcgg agaacaaagt ttttccctgg tccaaagagg cgctggaact tttatgcgaa
     3181 atggtctacc tcgaaaatga accggccggc gtggccgaga cggtgggtat cttggagaaa
     3241 atcttagacg aattgatcgc cgggggcaga gttcaaccca cggcgcagat agtcacttcc
     3301 ctgcgccagc tgctggagga ctttcgctcc aaaccgccgg aatgggcgcg gcccgttcag
     3361 gcggccctgg ataaggccgg cgacccgatt cgtctttcgc ggatgactga gctgctcaac
     3421 gccaataagg tagacgatcc ggaagcggtg gcccattacc tgcgcacctt agactggaaa
     3481 tcggtcggga acatccacgc gcttttgggg accctggagc actacccgca ccggaaactg
     3541 gtctgccagg tgttggagca aatgggcccg ccagcggtgg agttgatcgc caaggggatg
     3601 gccgatccgc gctggttcgt cgtccggaat acggccttga ttctggggca catcgggggt
     3661 gacaaggctt gccagcacct gcgggccggg ctcagtcact cggacgtacg ggtacggcgg
     3721 gagacgcttt tggccctgga gcagatcggc acgccgaccg cgagcaaaat ggtcttaggc
     3781 gtactcaacg agaaggatgg aaaactgcgg agcaaggcgt tgcagattct ggttcacctg
     3841 caggaagccg aagcctgtga accgctctgg ctgcgcattg agtcaaaaga ttttctgcag
     3901 tggtctccgg aggagcaacg cgaactggtt tttgcctacg cccaaatcgg ccgcgaccgg
     3961 gttctggaag aactggccgg actcgttacc cggcaggcgt ttttcaagcg cggactgtac
     4021 cagccgatca aagagttcgc cgtggaggca atggcgaagg tgggtaccct gcgggcccag
     4081 gagtacctgg agcgtctggc cgggacgacc catggcgcgc tgaaaaccgc ctgtcagacc
     4141 tgggctcagc gtctgaaacg ccgccaggtc cagggagttg ttcccgcaag aacagcggca
     4201 tcccggggag acgaacatga gtgacccgcg ctccgaattt ccgtcttcgg ccgccgtcga
     4261 gccgtcacac cccgatcccc gtcaaaggcg ggacaagagt tcggatctct ccgagaacat
     4321 gcgccggcaa ctggtggtgc agatgtacgt catcttccgc accgcccagt tgtacgagcc
     4381 ggcaaacagc gcctaccaga aggcgctgga ggcgctcctg cacacgctga gtgaatgctt
     4441 cgccatcgaa ggcgaggccc aaatcgtctt ccgccagggg tactggtacg tgaacggcgc
     4501 ccgcacccgt tttgaaattg acggtcggac cgccgcggag tctctggcac aactttttga
     4561 gcaacgtcaa atcggcggga tgggatttga accggcggtc agccgggagg agctcgtccg
     4621 ctttgccgat ctgtttctcc acaccgaggc acccacgcct gaggaacgtc tggcgcttct
     4681 gatgaaaaaa ttagatgccg cccaggtgtt aaccattatc cttttcagcc ccaatgtgtc
     4741 cccagccgag ggcttggccg accttttgac cctcgaatcg agccggcgtt cggcccggcg
     4801 caccttcttc tacgcggtca ccatggttga aggcttgatg cagcgcgcgc agcacgggga
     4861 gtcggttaac ttcctcaaag ccaagcgagt ggtgcactcg atcatcgacc acctcttgcg
     4921 cgatgaaggt gccttggtcg aactggcgac gttgaagacc tacgacgact atacctacgc
     4981 ccattcggtg aacgtgagtg tcctctctct ggcgttgggc ctccgcgtgg ggctggaccg
     5041 ccacaatttg agtcttctgg ggtttgcggc gctcttccac gacgtcggca agacgcggct
     5101 gccggtggag ttaatccata aaccgaccga atatgacgag atggactggc ggcagatgga
     5161 acagcatccg atcctgggcg ccaaagccat agcgctcacc cgcgcctttg acgagtacac
     5221 cgcgcgcggg atgctcgtgg ctttccggca ccatacgaat caagatctct cggggtatcc
     5281 caaactccgc cagccccggg cgtcggatct cttcacccgg atcgtcaaaa tcgccgattc
     5341 gtacaacgcc atgacgtcgg gacgggccta catcaaagaa tcgaacacgc cggacgaagc
     5401 gatccgcaaa atgcttctgg tttcgggcaa ggcgtacgat tccctcctgc tcaaggtgtt
     5461 cgtccaggtg ctgggcattt ttccggtggg gtccctggtg ctcctggaca cgcaggaact
     5521 ggccttggtc ttccgcgcca acaaaagcga cctctcccgg ccccaggtgc ggctcattgc
     5581 cgatgccacc ggcctcaaag aaggtctggt ggtggtcgat ctggccgaac gggaatctcc
     5641 cgaagggccc taccgccgca gcatcgtccg cgttttggat ccgaagaaat acggcctcga
     5701 tctttcccgg ttcgccctca attagcatct gatttcagga agttccaatc cggtacgaaa
     5761 gcttccgaga tttaagggtt tatacgccgg caatcttcag gcgactgcag ccgcttttta
     5821 ttccatagag gtcaagaatc ccgcctgacc cctccttcgc ccctcctttg gagagatgga
     5881 agaaaaaggg agaaaaaaat agcgcggggt tgtgaatgaa ccccgcgctg cgaactcgaa
     5941 tagtagcgcg actcacgtcg cgctacccct tagaagggat tacgttccct gttttttcgc
     6001 cgtgcagcag gccttgccat tcgtggacgt ggtggctttc acgtcgtgcg ggcaggggcc
     6061 gcagtcggtt tcggaaatct ccttgttttt catttcggcc ttgaagccgg cttttttcac
     6121 cgatcccatc acgcgcttca cggtggtggc gctgccatca tacgtgaccg tggcggtatt
     6181 ggtctccagg tcgacagagg cggcttcgac gccattggcc tttagaagcg cgctctccac
     6241 gcctttcttg cagccggcgc aggtcatccc cttgacgctt aaggtgacgg tcttcaaatc
     6301 tttgtcggcg gccagtacca aaggcagcgc gacaaaagcc agcgtgaaag cagtcagaac
     6361 gagtagtgcg aaactcttgc gcatgttcac ctcctcactg attcaggctc tcttcgattg
     6421 tgtcccgtac tgttttcaga gttttcctct gccttacacg gtatctgcaa ccgggaagac
     6481 tcacgtcatg tatacgaagc taatataacc tctggtggcg ttctgtcaag gcctcgcctc
     6541 ttcccaggca atcatttgag cactggtacc aaagcaggcg gggtcacaaa agttttttga
     6601 tttcctcttc aatttgagat ctgttcttgt agccaaaaaa ctttttgacc aggatccctt
     6661 ttcgatcgat gataaactgg gtcgggaaac cgacgatccc cccgtaggcc tttccgatct
     6721 tgtcgtcgcc catcaaaagc gggtaggtca aaccatgctt ctgtgcgaat ttggccacgt
     6781 ctttggcgct gccgctggca aaggcgacgc cgatcaccac cagccccgag tctttgtagg
     6841 tggcgtagag ctctttcaaa tgcggcagtt ccataataca ggggccacac caggtggccc
     6901 agaagtccaa aaagaccacc ttgccggcaa aatcggcgag cgtgaccggc ttgcccgcga
     6961 ggttgtttaa gacgaaaccc ggggccttct ggcccgcttc ctgagccaca gccagtccaa
     7021 aaaacagagc cgtggcgagg ctcagtactc caatcccgac gatgtggcga cgcaatccgg
     7081 acacgcttcc tccgttattc tcttaaaata aactaccgtt gctgactatg ccgagcgggg
     7141 ggctgaggaa aactcgacat agtgcggaga gcaacggtag attctcataa actaatacgg
     7201 acaaatcagc ttttcgattt gccctcctgt tgtttgaggt agtcgtcaaa ttccattttt
     7261 tcgtacaggt cgtctttgat gtaattgatg atgtgaacga actggtcggg gcccacgtag
     7321 ccgggcaggg gggttatcgc ctcgccgttg ggtttcaaaa accaaatggt gggatagccg
     7381 gtcacgccgt atttgccgga agctaactgg gcttcggtca tggtctttcc ttcgttttca
     7441 aaggaccgat tcgattcggc gttcaccttt accgccacgt agtgtttgcg caggatcgtt
     7501 tgaactttgg ggttggcgta ggtgtcctta tctaattttt tgcaccagcc gcaccaggta
     7561 gtgtagaaat cgaccataac gtgtttgttc tctttcgccg cctgtttcag tccttcctca
     7621 tacgttaacc agaccacctc ggcgttttgt tccgtcggtt tggccgattt tttgacggac
     7681 ttatcggcgg cctttttctg agcccccaga tgtcccgtta atacaagggt caacgccaat
     7741 acccccactc ccacggtgca gaatcgtttc atatgtcacc tctctctacg tgacggcccc
     7801 gatgcgcgtt ggctacgaaa tcgtttgact gtcgcccgcc ccgtatgacc gagcaaaatc
     7861 gctgccagca ccaatcccga aaactgcgcc acaccgttgg gaattatatc ggcaccggcg
     7921 gcgaccttct ccatagacgg agtgcccatt ccgcgataga aggcattcac cgccagaccg
     7981 agcagcaggc tggtaacgac aatagaaagc aggtacagta ccaagctgcg tttgcccagg
     8041 aatttggcga cggcgaccat cgtggtcgta tttgttgccg ggccgactaa aaggaaaacc
     8101 aaagccgcgc cgggggaaag acctttgagc aaaagcacac tcgccaacgg cgtggctgaa
     8161 gttgaacaaa tgtacagggg cgtgctgaca ccggccatca aaagcattgc ggtgaagtcg
     8221 ctccggccaa gggtccgggt tatccaattg gccgggagca aagccgagag agccgccgag
     8281 agcagaaagc cgaacacgag atacagggca atgtcagcca ataggtcgcc gaagccgtag
     8341 cggattgctt gcgcaaagcg cttaagccag gaaagccgca aagtcgtcgc ccccggtaag
     8401 gtcgtcaccg ataagggaag ccggttttcc ttttggccga aaaggagttc gaccgttcca
     8461 gcggccaatg aagtcaaaac tgccgccacc gggcgcagaa tcgtcaaaaa cggatcgagc
     8521 aaggcatagg agaccgcgat ggaatcgacg ccggtttcgg gggttgagat cagaaatgag
     8581 acggtcgccg gtttggaggc gccgtccttg cgcagtccga ccgctgtggg gatgaccccg
     8641 caggagcaga ggggtagagg tgtgccgatc agtgccgcct tggctaccgg cccgaagccc
     8701 ttccctgata ggtgccgggc gatggtcttg gcggggatga actcgtgaat cagcccggcc
     8761 agaaggaaac cgcccaaaag ccagaaggcg gagtcaacca ccaggtcggc aagagatttg
     8821 aaaaataggg ctaccgaatg ctcaatcatc ttctcgttct atcacttgag gatacgtttt
     8881 gcgccttcgt ttttacgcaa aatatagcat agatacaccg tagcaattcc tgcaaacgga
     8941 gggggacccg ccacgtaggt acaatagtat atatgcgcaa ttaagcaact atttaacggg
     9001 gataccatgg agaaatttgc caaccttttg aaaacactgg gtgaaccgat ccgcctgcgc
     9061 atcctgcgtc tccttttggc cgcccagcgg gaactctgcg tctgtgagtt cgtggatgct
     9121 ctcgaggtgc ctcagtacaa cgtctccaag cacctccacg ccctgcgcgg ggcggggctt
     9181 ttgaccctcc gcaaggaggg gcgctgggtc tacttttcat tggcccaaaa aaccagcttt
     9241 cagaaatctc tcctgaaagc gctctcaggt ctctctgatg agattttgga tcaagataga
     9301 aaagaattgc agaagcggct ggcattgaga gtgaaggaca aatgtctttt ggggattcag
     9361 aagaaaaagc tattggttgc cggcctgggc gcccagcttg aatgaaaccg ttcatccttc
     9421 tgggtttgtc tggcctcctg ctccatggta cggcgatggc tgccaagccg cagatgtccg
     9481 gcggggttga tctccgttat gagaaccgga acgggaataa acattccctg agggtcgaag
     9541 gagtattcct caatatgcga aaagtcttta gtgaccagga aggagatagc ctcatcaccg
     9601 taggacaaat cgactgggat gacaattttg accggttgcg accataccag acgtatgctc
     9661 agtacaaagg acctttgggc aagtggaacc tgtcagcggg gcattttatc ctcccctacg
     9721 gtcttttgtc cgactacgac actgagcggc tgctcttgca aactcaggaa ttgaaagcct
     9781 taggcatcaa attggatacg gggttgaaat gctcaggccg tttcagaact gttgactacg
     9841 ccttctcggt cactcagggt gtcggtcgca ccagactgga agacgcggac gggaacaaac
     9901 tggtaaccgg aagaattggc tgggcaggaa atgatgtgag cacggggttc tctatcctga
     9961 acgggaaagt tttgattgac ggggatgata gaactctccc ggtgtatgag agaagggcgg
    10021 gatttgacct gacgaaatat tcgggcccga cgacggttcg cggtgaactg acctggggaa
    10081 gaaacaatgg ccagacgacc tacagcgggt acttcggaat ggatcacgcg ctttcaacca
    10141 aggtagaggt caatttcaag tacgcctact ggcaggggaa tgcgaccgag cattttctgg
    10201 gagtgggatt ttcatacaat ctttgggcaa gggtaattct cagggcagcc gatgaatacc
    10261 aattcagcca aagggaccag aatgcatttt ctctccaagt gtactgggat tttctcaaaa
    10321 tcctgtaaag ttgtcttagt gctctgggtt tttgcactga gttccgcttc tgcagatgtg
    10381 tgcgtatggc ggaacccgga aagaactatg gtcaatatct ttccacaagc caaagattac
    10441 cgaaccgtgg acaggaaaat tagcgaggag aaacgggcaa tcatcgagaa gcaacttggc
    10501 gagaaactcg cgccgggaga acgggaagat tggacctact atgaaattat ggggaaagaa
    10561 aatggactgt tgggcttcat cagtgctgat gcggagaagg gcgaattcgg tgttattgag
    10621 gtcgtcgtgg ggattacgcc agaggggaaa gtgaaaggcg tctacatcca gagagcccgg
    10681 gaaaagaata aggaattcaa atccagagaa tttttaagtc aattcgaagg gaaaaccgtc
    10741 aaagattcgc ttttgatcgg aaaaggtata caggtaaaag aacggtcgct cccggtgcaa
    10801 atgaccgtgt tgggagtacg aaagatgctc atcttttatg accagttagc caacccccgc
    10861 agatgaaagg gaggtcgcag tgaaaaaaat cggtatcatc tcggcagttg ggcttgcggt
    10921 tttgctcggt gcggtttcgg taacggccaa gacgaagaaa atgggtaagc gcatcccgga
    10981 aaaggccacc gtcgttaaac tgagtgacat tctgaaagcc ccggaaaagt ttaaggacaa
    11041 ggaagtgatc ttacaaggga cgtacgtcaa ctactgctgt gccaccgact tcgtctaccg
    11101 cgaaggtctg gagtcggtgg aaatctaccc gcaagggttc ctcactccca agctggacaa
    11161 agggaagcaa atgaaggtct ggggaattgt gcgagtcagg gagagggagg agggagaaca
    11221 gcaggaaacg aggaaaaacg ctaaggaaga agaacacgcc gagattatta tcgaagccga
    11281 aggcctggag gtgaaatgac catcttttct ctggcgttga aaaatctcaa gagacatagg
    11341 accagaacta tcctgaccat tctggggatt gccgtcgctg cagccacgct cttttccatt
    11401 ctctcgtttg atgcaggtta taagaaggcc ctgcaggagg aactggccgg ctcgggaatt
    11461 cacctgtttg tctcgaccga aggctgcccc ttggaagcgg cttcgctcat catccacggc
    11521 ggcgagatac cccgatttct gaacatgcag acgttcaacg cggtgaaaga cattccaggg
    11581 gtgaaacaag ccggcgggtt tctcattttc tccctcccct c
//
LOCUS       DBNGZD010000356         5138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_959585, whole genome shotgun sequence.
ACCESSION   DBNGZD010000356 DBNGZD010000000
VERSION     DBNGZD010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_959585"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..793)
                     /gene="atpC"
                     /locus_tag="AC16GM_09445"
     CDS             complement(380..793)
                     /gene="atpC"
                     /locus_tag="AC16GM_09445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR2923931.1"
                     /translation="MFELSIATPEKVFYEDQVESVTAPGADGYLGVLAHHAPLITTLL
                     PGKVIVRDKEKQTIVFAVSGGFLEVSRNKATLLADAVELAEQISVDRARRALARAQER
                     IHKKEPGIDLSRALEALKRAENRMRIHKESYPQRA"
     gene            complement(926..2395)
                     /gene="atpD"
                     /locus_tag="AC16GM_09450"
     CDS             complement(926..2395)
                     /gene="atpD"
                     /locus_tag="AC16GM_09450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR2923932.1"
                     /translation="MAEVAVREPKVKPKPVTEKKTEKNWGKVVQVIGPTVDCEFPSDR
                     LPNMLSAIKIEDEKRGIKLTVEVALHIGDNVVRCVALSSTDGLVRGMPALDTGGPITV
                     PVGPQTLGRIFNLLGDPIDEQGPLPSGNKRYPIHRPAPSFEEQETKIQLFETGIKVID
                     LLEPYPKGGKVGMFGGAGVGKTVILQELIHNIATEHGGYSVFSGVGERTREGNDLWLE
                     MKASGVLAKTCMVFGQMNEPPGARLRVGLSGLTMAEYFRDEEHQDVLLFIDNIFRFVQ
                     AGSEVSALLGRMPSAVGYQPTLGTVMGALQERITSTKSGSITSVQAIYVPADDLTDPA
                     PATTFSHLDATTVLSRQISELGIYPAVDPLTSTSRILDPKVVGEEHYDVARAVQKILQ
                     RYKDLQDIIAILGMDELSEDDKVIVARARKIQKFLSQPFFVAEVYTGTQGRYVKLTDT
                     IRGFKMIVEGQLDHLPEQAFYMLGQIEEAIERGEQLLKR"
     gene            complement(2501..3361)
                     /gene="atpG"
                     /locus_tag="AC16GM_09455"
     CDS             complement(2501..3361)
                     /gene="atpG"
                     /locus_tag="AC16GM_09455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR2923933.1"
                     /translation="MPSIRDLRRRIRSVENTKQITRAMQMVAAARLRRAQAKVEAARP
                     YASKMQEMLASLASAATALEHPLFQKREVRKVGLVVITSDRGLCGSYNANVIRNAELF
                     LRERAPDNTLLALVGKKGFDFYHRRRWELRLKYLDLGGNLVLTRTRRIASDLTNLFLS
                     GEVDEIHLLYTRFVSTVTYRVTREKFLPIEPQKSAGDSAGALDYIFEPGPEQIFQTLL
                     PRFCLTRVQIALAEAFASEHGSRMIAMTSATQNALEMIDHLTLVRNKVRQASITKEML
                     EIVGGAEALK"
     gene            complement(3483..5000)
                     /gene="atpA"
                     /locus_tag="AC16GM_09460"
     CDS             complement(3483..5000)
                     /gene="atpA"
                     /locus_tag="AC16GM_09460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR2923934.1"
                     /translation="MAIRPEEVSAIIKKEIERYETKLEMESVGTVLQVGDGIARVWGL
                     EDAMMSELVLFPGEVTGMVLNLEADNVGVALFGSDRGIKEGDTVRSTGRIAQVPVGEA
                     LIGRVVNALGQPLDGKGPIVTDKFRPMEGHAPNVVQRQPVNQPVQTGLKAIDSMIPIG
                     RGQRELVIGDRQTGKTALVLDTIINQKGKDLFCIYVAIGQKQSTIAQVVETLTGYGAM
                     EYTTVVAAAATDPAPLQYISPYAGCAIGEEFRDIGRHALCVYDDLSKHAQSYRQLSLL
                     LRRPPGREAFPGDIFYLHSRLLERAAKLADQRGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYAGVRPAINVGISVSRVGGKAQVKAMRKVAGRLRLDLAQYREL
                     AAFAQFGSDLDETTKAQLIRGERTVEILKQDQYVPMPVEKQVMIIWVATHGHLDDLLI
                     DAVKRFETEFYAFMEKEYPEVPHTIAQTKELDEKIEKRLEEATQKFKEKFKSNLK"
BASE COUNT          978 a         1604 c         1411 g         1145 t
ORIGIN      
        1 gcaagaggac gagcccatcc ccgccgacgc cggtatgccg gtcgctgacc aaccgggcca
       61 ggcggttgaa attggcccgc accggccggc ggatggtatc gatgaagatg tagtcgtttc
      121 ccgcgccgtg cattttcaca aactcgattt cggccattct tctgaattta acgcattgct
      181 cctaaaaaca aaaagaccac tacttttccg actgtagtgg gcgcgcaagc cgcgttttag
      241 ccggttctca cccactactt ctgacagcat accaccacgc ccagctaaga cgctgggagc
      301 ggaaagtcag aagttgggcc aagaccggat tcataatctg catacaagat ataacgactg
      361 ccgtatttgt caagtccttt catgcccgtt gggggtaact ttccttatga attcgcattc
      421 ggttttccgc ccgcttcagg gcttccaacg cgcgggagag gtcaatcccc ggctcctttt
      481 tgtggattcg ctcctgcgcc cgggctaagg cccgcctggc ccggtcaacg ctgatttgtt
      541 ccgccaactc cacggcatcg gccaaaagcg tcgccttgtt gcgggagact tcgagaaagc
      601 cgccggacac cgcaaaaaca atcgtctgtt tctctttgtc gcggacgata acctttccgg
      661 gcaaaagcgt cgtgatgagc ggcgcgtggt gggccagaac gcctaagtaa ccgtccgcgc
      721 cgggagcggt gaccgattcc acttggtctt cgtagaagac tttttcagga gtagcaattg
      781 aaagctcgaa catcaccttt tcaacctcgt ccggctcagg gacgagccgg actaccgagt
      841 gctcttgcgc ggcagtggta gccgcggtcg tccccgagcc cggctgtccg acaaatcgga
      901 cgacttaatc tcaccaacag tttggctacc gcttcagcag ttgttctccc cgctctatcg
      961 cttcctcaat ctgccccagc atgtagaacg cctgctccgg caggtggtcc aattgtccct
     1021 cgacaatcat cttgaacccc cggatcgtgt cggtcagttt gacgtaccgt ccctgggtgc
     1081 cggtgtagac ctccgccacg aagaagggct gagagaggaa tttttgaatc ttccgcgccc
     1141 gcgccacaat caccttgtcg tcttcggaga gttcgtccat ccctaaaatg gcgatgatgt
     1201 cctggaggtc tttgtagcgc tggagaattt tctgcaccgc ccgcgccacg tcgtagtgtt
     1261 cctcgcccac cactttgggg tcgagaatgc gcgaggtcga ggtcaaagga tcgaccgccg
     1321 ggtaaatgcc caactcggaa atctggcgcg agaggacggt cgtggcatcg aggtgcgaga
     1381 aggtcgtcgc cggcgccggg tcggtcaggt cgtcggccgg gacgtaaatc gcctgcaccg
     1441 aggtgatgga acccgacttg gtcgaggtaa tccgctcctg cagcgcgccc atcacagtcc
     1501 ccaaggtcgg ctggtagccg accgccgacg gcatccgccc caacagcgcg gagacttcgg
     1561 agccggcctg cacgaagcgg aagatgttat cgataaagag cagcacatcc tggtgctcct
     1621 catcccggaa gtattcggcc atggtaaggc cggaaagccc cacccgcagc cgcgcgccgg
     1681 gcggctcgtt catctggccg aagaccatgc aggttttggc gaggacgccc gaggctttca
     1741 tctccagcca gaggtcgttc ccttcgcggg tgcgctcccc caccccggag aaaaccgagt
     1801 agccgccgtg ctcggtggcg atgttgtgaa tcaactcctg caggatgacc gtcttcccca
     1861 ctcccgcccc gccgaacatc cccaccttgc cgcccttggg gtacggttcg agcagatcga
     1921 tgacttttat gccggtttca aacaactgaa tcttcgtttc ctgttcttcg aaagacggcg
     1981 ccgggcggtg aatgggatag cgtttgtttc ccgacggaag cggaccttgc tcgtcaatgg
     2041 gatcgcccaa gagattgaaa atgcgtccca gagtttgcgg cccaaccggc accgtgatgg
     2101 gtccgccggt gtccagcgcc ggcatcccgc gcacgagacc gtcggtggag gagagcgcca
     2161 cgcagcgcac aacgttgtcg ccgatgtgca gcgccacctc gaccgtcagc ttaatgccgc
     2221 gcttttcgtc ttcgattttg atggcgctaa gcatattcgg cagccggtcc gagggaaact
     2281 cgcagtccac cgtcggcccg atcacctgta cgaccttgcc ccaattcttt tcggtcttct
     2341 tctccgttac cggcttgggt ttgactttcg gttctcgcac cgctacttca gccatgcact
     2401 ctccttaatg gtaataagtt gtttctgtat ttttcccaaa tttattttgt tccggccagt
     2461 gatctttccc agtgtccggt ctcctgtgtt ctgcatgctg tcatttcagc gcttccgctc
     2521 ccccgacgat ttccaacatc tctttggtaa tcgacgcttg ccgtaccttg ttccgcacca
     2581 aagtcaggtg gtcgatcatc tctagggcgt tctgggtggc cgaagtcatg gcgatcatcc
     2641 gcgaaccgtg ctcggaggca aaggcctccg ccaaggcgat ctgcacccgc gtcagacaga
     2701 agcgcggcag aagcgtctgg aaaatctgct ccggacccgg ttcaaagata taatccagcg
     2761 cacccgcgga gtcacccgcc gacttctgtg gctcgattgg caaaaacttc tccctcgtga
     2821 cgcgataagt aaccgtcgag acgaagcggg tgtagagcag gtgaatctca tccacctcac
     2881 ccgacaaaaa gaggttggtg aggtcggagg caatccgccg cgtccgggtt aatacgaggt
     2941 tgccgcccag atcgaggtat ttgaggcgca gctcccacct gcgccggtgg tagaaatcaa
     3001 atcctttttt gccaacgagc gcgaggagcg tgttgtccgg cgccctctcg cgcaggaaca
     3061 attccgcatt gcggatgacg ttggcgttgt acgagccgca cagcccccgg tcggaagtaa
     3121 tgaccaccag ccccaccttt ctcacctcgc gcttttgaaa gagcgggtgc tccaaggccg
     3181 tggccgcgga agccagagat gccagcatct cctgcatctt agaagcgtaa ggccgggctg
     3241 cttccacttt cgcctgcgcc cgcctgaggc gggccgccgc caccatttgc atggcccggg
     3301 tgatctgctt ggtgttttcg accgaacgaa tccggcgtct caagtcacgt attgacggca
     3361 tgacgtatca taaatatgac tcttttgcgc atctcttttt tgtagcgcgg gatggtcttc
     3421 gtatcccgcg ctggcgcagg ttccagaaac caacctgcgc tacaagtctc tagaaactgt
     3481 tactacttca aattactctt gaacttctcc ttgaacttct gcgttgcctc ttccaaccgt
     3541 ttttcaatct tctcatccaa ttctttcgtc tgcgcgatgg tgtgcggcac ttccgggtac
     3601 tctttttcca taaaagcata gaactcggtc tcgaaccgtt tcaccgcgtc gatcagcagg
     3661 tcgtccaaat ggccgtgggt cgccacccag atgatcatca cctgcttctc caccggcatc
     3721 gggacgtatt gatcctgctt taagatttcc accgtccgct cgccgcggat caactgcgcc
     3781 ttggtcgttt cgtctaaatc ggagccgaac tgggcgaagg ctgccagttc ccggtactgg
     3841 gccaaatcga gccgcaaccg gcccgccact ttccgcatcg ccttgacctg ggctttgccg
     3901 ccgacgcggg aaactgatat gccgacgtta atcgccggcc gcaccccggc gtagaagaga
     3961 tccgactcta agaaaatctg gccgtcggtg atggaaatca cgttggtcgg aatgtaagcc
     4021 gacacatccc ccgcctgcgt ctcgatgatc ggcagggcgg tcaaggagcc gccgccgcgc
     4081 tggtcagcca acttcgctgc ccgttcgagc aagcgggagt gcaaatagaa gatgtcgccc
     4141 gggaaggcct cccgccccgg cggccggcgc aacaggagcg agagctgccg gtacgactgc
     4201 gcatgctttg aaagatcgtc atagacacag agggcatgac ggccgatgtc gcggaactcc
     4261 tcgcctatgg cgcagcccgc gtacggggaa atgtactgca aaggcgccgg gtcagtcgcc
     4321 gcagccgcca caacggtggt gtactccatt gcgccgtagc ccgtaagggt ctccaccacc
     4381 tgcgcaatgg tcgattgctt ctgtccgatg gcgacgtaaa tgcaaaagag gtctttaccc
     4441 ttttggttga tgatggtatc taggacgagc gccgtttttc ccgtctgccg gtcgccgatc
     4501 accagttcgc gctggccccg gccgatggga atcatcgagt cgattgcttt caacccggtt
     4561 tgcacgggct ggttcaccgg ctggcgctgg acgacgttcg gcgcgtggcc ttccattggc
     4621 cggaatttgt cggtgacaat cggccctttg ccgtccaggg gctggcccag cgcgttcacc
     4681 acccggccga tgagcgcctc ccccaccggc acctgcgcga tgcgcccggt gctccggacc
     4741 gtatcgccct ctttgatgcc ccggtcggag ccgaagagcg ccacccccac gttgtcggcc
     4801 tcgaggttca aaaccatgcc ggtgacttca ccggggaaga gcaccaattc agacatcata
     4861 gcgtcttcca aaccccagac ccgggcgatt ccgtccccca cctggagcac ggtccccacc
     4921 gactccattt cgagtttggt ctcgtaccgt tcaatttctt ttttgatgat cgcgctgact
     4981 tcttccggcc gtatcgccat ttactggtct ccgttcagtg ctcgcggtca ggcaagatgc
     5041 ctgacctatt cgggattttg gccttccctc aattctcagt cctcaatgct gattttctaa
     5101 tgcactttga ctgccagaag ctgttctttg agttcaac
//
LOCUS       DBNGZD010000357         1609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_751187, whole genome shotgun sequence.
ACCESSION   DBNGZD010000357 DBNGZD010000000
VERSION     DBNGZD010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_751187"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..130
                     /locus_tag="AC16GM_09465"
     tRNA            34..130
                     /locus_tag="AC16GM_09465"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68..70,aa:Sec,seq:tca)
     gene            140..1537
                     /gene="selA"
                     /locus_tag="AC16GM_09470"
     CDS             140..1537
                     /gene="selA"
                     /locus_tag="AC16GM_09470"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR2923935.1"
                     /translation="MADTKKTAPGKSSALRQLPSVEKLLEAPQVRAFADQLPQPVVAE
                     VIRTVVDECRKKIDSGQGKVDLPALLATLQTRLVAKTRQALEQVVNGTGVILHTNLGR
                     APLGEEVIAAMAQAARQYVNLEYRLSDGTRGSRGAYAEELLCQLFGCEAAAVVNNNAG
                     ALFLALSAMAKGKEVVISRGELVQIGGGFRIPEILAESGAVLREVGTTNQTELADYRR
                     ALGPQTGLILKVHSSNFLQVGFVRSVSVRDLVLLGQEHGVPVMEDIGSGAVPDTAEFG
                     LKTEPTVGGAVSAGCNVVSFSGDKLFGGPQAGILVGKKEIVERLKKHPLYRALRVDKI
                     IFAAIEVLARQYLTKATDKIPVWQMITLPIDTLQKRAQVLAKELTGDKITVRVVECRS
                     TVGGGSLPGETIPSSGLEFESEVSPHEIAAVFRGQSPPVIGRIENDRFLIDLRTVFPD
                     QDKSLVTAAQQVFKK"
BASE COUNT          339 a          453 c          477 g          340 t
ORIGIN      
        1 gccccacgga agcgggcgtg gtagtttgcc cttggaagcg gatcggatct ggtggtccga
       61 gcggacttca aatccgtatg cggctgcgtt cgcgtggtcg tggtaggttc gattcctacc
      121 ccttccgcca catttgatta tggctgacac aaagaaaacc gccccgggca aaagctcagc
      181 actgcggcaa ttgcccagcg tggaaaaact tttggaagca ccgcaggtgc gggctttcgc
      241 cgaccaatta cctcagccag tggtggctga ggtgatccgc acggttgttg atgaatgccg
      301 aaaaaaaatt gattcgggcc agggaaaagt tgatttgcca gcactcctgg ctacgctcca
      361 gacgcggctg gtggcgaaaa cgcgccaggc cctggaacag gtcgtcaacg gcaccggggt
      421 gattttgcat acgaatttag gacgggcacc tctgggtgaa gaagtcatag cggcgatggc
      481 gcaagccgct cgccagtacg tcaatttgga ataccggctc tccgacggca cccgcggcag
      541 ccggggtgcc tatgccgagg aactcctctg tcaactcttc ggctgcgaag cggcggcagt
      601 ggtcaacaat aacgccggcg cgttgtttct ggcgctctcc gccatggcga agggaaaaga
      661 agttgtgatc tcccgcggcg agttagtgca gatcggcggc ggcttccgca ttccggaaat
      721 tctggccgaa agcggagcgg ttttgcgcga ggtcggcacc accaatcaga ccgaactggc
      781 cgattaccgc cgggcgctgg ggccgcagac cggcctcatt ttgaaagtcc actccagcaa
      841 tttcctgcag gtgggcttcg tccgctcggt ctccgtccgt gacctggtgc tcttaggtca
      901 ggagcacggg gttccggtaa tggaggatat cggctccggc gcggttccgg acaccgctga
      961 gtttggactg aaaaccgagc ccaccgtcgg cggggccgtt tccgctggct gtaatgtagt
     1021 cagtttcagc ggcgataaac tgttcggcgg cccgcaggcg gggattctcg tcggtaaaaa
     1081 agaaatcgtg gaacggttga aaaaacatcc gctctaccga gccttgcgcg tcgataaaat
     1141 catctttgcg gccattgaag tgcttgcgcg ccagtatctc accaaagcca ccgacaagat
     1201 tccggtctgg cagatgatca cactgcctat tgacactctc caaaaacgcg ctcaggtact
     1261 ggctaaggaa ctgaccggag acaagatcac tgttcgggtt gttgagtgcc gcagcaccgt
     1321 gggtggcgga tcactgcccg gtgaaaccat tccttcctcc ggtcttgaat ttgagagcga
     1381 agtttccccg catgagattg cagctgtgtt tcgcgggcaa tctcccccgg tgatcggccg
     1441 tattgaaaat gaccgttttc tcatcgacct ccggacggtt ttccctgacc aagataaaag
     1501 tctcgtcacc gccgctcagc aagtcttcaa aaaatagata ggtcttatgt tcgtcttagg
     1561 caccgccggg cacgtggacc acggcaagtc aacactggtt acacgcctc
//
LOCUS       DBNGZD010000358         2794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_56461, whole genome shotgun sequence.
ACCESSION   DBNGZD010000358 DBNGZD010000000
VERSION     DBNGZD010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_56461"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..791)
                     /locus_tag="AC16GM_09475"
     CDS             complement(36..791)
                     /locus_tag="AC16GM_09475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR2923936.1"
                     /translation="MISADITHFFVEPPAVEENRLSLTGPEAFHCASVLRHKVGDRIT
                     CVDGQGYTYTVEIVQVKKGLVAGKILHRTVNRNEPKIKVTLAQGVTKVSKFDWVIEKG
                     TEIGVSAFLPLLTEKSVAKRTDLEVWAQKAARLKRVALAAMKQSLRSVCPAILPPVKV
                     DELARRLSDYDLSLLASFRPDAQPIFQIFSQRPSKPNRILLTIGPESGLSPREEDSLL
                     SSGVVPVSLGPRRLRTETAGLVLASQVLGWAGD"
     gene            complement(919..1791)
                     /locus_tag="AC16GM_09480"
     CDS             complement(919..1791)
                     /locus_tag="AC16GM_09480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR2923937.1"
                     /translation="MAYHELAVRLPAKYHDAVSNFLITRGVNGLITKDGSHWLIGYAF
                     DRQSLAEKKGALLAYLRSLQKLNHPFPFRITTREIPRWDWSQRWKESFAPKELSCGIV
                     IKPPWVRRAFPGKLVITINPKMAFGTGEHATTQLCLSAVYALTRPGMRVLDVGTGTCI
                     LAIAAVKLGAKTAVGCDTDPLAIANAWENCRLNKVEKRIRLAQGSLEQIRARKTYDLV
                     LANLITNQVVEFLPDLVRRARPWGYLVLSGVLARQQRRVLDSIAKQSLDLTAIGEQQE
                     WLCCIAQKTSLRGE"
     gene            complement(1795..>2794)
                     /locus_tag="AC16GM_09485"
     CDS             complement(1795..>2794)
                     /locus_tag="AC16GM_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR2923938.1"
                     /translation="DPEKRRRYDQFGHVGVGAGAGAGAGGFSDFGFGGFDLSDALRAF
                     MRDFGGFEEFFGAGTPRRSRGERGGATRGRDLQVRLQLTLEEIANGAEKTIKLRRLVK
                     CETCNGSGAEKGSSKRTCPTCRGSGEIRQVSRSLFGQFVSVSTCTTCGGEGVVVERPC
                     AACGGEGRVRGQSTINVKVPAGVATGNYIPIRGAGDVGPKGGPAGDVYVLIEEEEHPV
                     FTRHENNVICEIPISMIQAALGDEIEVPTLDGRAQIKIPAGTQSGKIFRLKNKGIPHL
                     HAHGKGDELVRVVVWTPTSLSSEDKRLLRELEKSENLRPPKADKSFFTRLRESLGF"
BASE COUNT          557 a          823 c          824 g          590 t
ORIGIN      
        1 cctcggctac tcgaattctg ggttgcgtaa gggaattaat ccccggccca gcccaggacc
       61 tggctggcca agacaagacc ggcggtttcg gtgcggaggc ggcgggggcc taaagatacc
      121 ggaacgacac ctgaggaaag gagagaatct tcttctcgcg gagaaaggcc gctctccggg
      181 ccgatggtga gcaaaattcg atttggtttc gacggtcgct ggctgaagat ctgaaaaatg
      241 ggctgagcat cgggtcgaaa agaggccaga agggataaat catagtcgct gagccgccgg
      301 gccaattcgt ccactttcac cggcggcaga atagccgggc agacggagcg gagggattgc
      361 ttcattgccg caagggcgac gcgcttgagg cgcgcggcct tctgggccca gacctcgagg
      421 tcggtccgtt tggcgaccga tttttcagtc aaaagcggta ggaaagcgga gacgccgatt
      481 tcggtcccct tttcaatcac ccagtcgaat ttgctaactt tcgtcactcc ttgcgctagg
      541 gtgactttga ttttcggctc atttcgattg acggttcggt gcaggatttt ccctgccacc
      601 agcccttttt ttacctgcac gatttcaacc gtgtaggtgt aaccctggcc atcgacgcag
      661 gtgatccggt cgccgacttt gtgccgcagg acagaggcac agtggaaggc ctccgggccg
      721 gtgagggaaa gtcgattttc ctctaccgct gggggttcga cgaagaagtg ggtgatgtcg
      781 gccgagatca aagtcgctgt ctcatatctg cacgaccggc ttagggacga gcccatacgg
      841 gttgcaccaa ccggtctacc agagaaaagt tgatttttga cccctccctc gccctcccct
      901 ctaaggggag ggaaaaatct actctccacg tagagaagtt ttctgcgcaa tgcaacagag
      961 ccactcctgt tgttcaccta tggcggtgag gtcaagcgac tgttttgcaa tagaatccag
     1021 cacccggcgt tgctggcgcg caagcactcc cgagagaacc aaatagcccc acggtcgggc
     1081 acggcgaacc aaatcgggca agaattcaac cacctgattg gtaatcaggt ttgccagaac
     1141 caggtcgtac gtttttcttg cgcggatctg ttcgagcgat ccctgcgcca atcgaatacg
     1201 cttctcaact ttattcaggc ggcaattctc ccacgcgttc gcaatcgcca gcgggtcggt
     1261 atcacaaccg acggcagttt tagctcccag tttcaccgcc gcaatcgcca gaatgcatgt
     1321 gccggtgccg acatccagaa ctcgcattcc gggacgcgtc aacgcataca ctgcggagag
     1381 acacaattgt gtggtggcat gctcgccggt accgaaggcc atcttgggat tgatggtgat
     1441 gaccagtttt cccggaaagg cgcgcctgac ccagggcggt ttgatgacga tgccgcagga
     1501 taactctttc ggcgcaaaac tttccttcca cctctgcgac caatcccagc gcggaatttc
     1561 ccgggtggtg atgcggaagg gaaacgggtg attcagtttc tggagacttc gaagatatgc
     1621 aagtaaggcg ccttttttct cggcgagact ttggcgatca aaagcgtaac cgatgagcca
     1681 gtgggaacca tcctttgtta tcaaaccatt gacgccgcgg gtgatgagga aattgctgac
     1741 ggcgtcgtgg tatttggccg gcagacgcac cgccagttca tggtaggcca tgccttaaaa
     1801 acccagactt tccctgagac gggtaaagaa ggacttgtcg gctttggggg gcctgagatt
     1861 ctcgcttttc tccagttcgc gcaggagccg tttgtcctcg gacgaaaggg aggtcggcgt
     1921 ccagaccacc acccgcacca gttcgtcgcc tttgccgtgt gcgtgaagat gcggaatccc
     1981 tttgtttttc aagcggaaga ttttcccgct ctgcgtcccg gcgggaattt tgatttgcgc
     2041 ccggccatcc agagtcggca cttcgatttc atcccccagc gctgcctgaa tcatgctgat
     2101 cggaatctcg cagatgacgt tattctcgtg gcgggtgaag acgggatgct cttcctcttc
     2161 gattaagacg tagacgtcgc ccgccgggcc gcctttgggg ccgacatcgc ccgcgccgcg
     2221 aatcgggata tagttgccgg tggcgactcc cgccgggact ttgacgttga tggtcgactg
     2281 cccgcgcacc cggccctctc ccccgcaggc ggcacaggga cgttcgacca cgaccccttc
     2341 cccgccgcag gtggtgcaag tggaaacgct tacgaactgg ccgaagagcg agcggcttac
     2401 ctgccggatt tcgccggagc cgcggcacgt gggacaggtg cgcttggacg atcctttctc
     2461 ggcgccgctg ccgttgcagg tctcgcactt taccagccgc cggagtttga tcgtcttctc
     2521 cgcgccgttg gcgatttctt ccagggtcaa ctgcaaacgc acctgcagat cgcgcccgcg
     2581 cgtagccccg ccacgttctc cccgactgcg gcgtggcgtg ccggcgccaa agaactcctc
     2641 gaagccgccg aagtcgcgca taaaggcgcg cagggcgtcg gagagatcga agccgccgaa
     2701 gccgaagtca ctaaatccgc ccgcgcctgc tccggcaccc gcgccgacac ccacgtggcc
     2761 gaactggtca tagcggcggc gcttctccgg gtct
//
LOCUS       DBNGZD010000359         1491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_544116, whole genome shotgun sequence.
ACCESSION   DBNGZD010000359 DBNGZD010000000
VERSION     DBNGZD010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_544116"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..>1491
                     /locus_tag="AC16GM_09490"
     CDS             55..>1491
                     /locus_tag="AC16GM_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR2923939.1"
                     /translation="MTKVQVSRILVVDDEQYICDIVREALVSHPEYQIQTTADPRRAV
                     EILTNEPVDLVLSDLIMGKITGVEILEVARKLHPDSIVILMTGQPTVENAVEVIKQGA
                     YDYLIKPFTLETLRATVERGLERQRLARENVHLKEQMGLFRISEAMGSTIHLDQLLTL
                     MLDAIIKEFDADMASILLVDKITGELKLNAFRGNTSDSAGSWFLLGHDEVCRWVVDNG
                     LPKMVNEKQERRRRTSPGLKQNVKSFVCYPLLARGKVLGVLNVVRTEKMAEFSQGQLQ
                     SLGIIVSKAASAIESSMLYEELQDAYLDTIIVLANAIEARDSYTRLHTDRVRYLAELI
                     ARELGWPDARMREVWMGGILHDVGKIAVPDGILNKRGPLSPEEFTIMRQHPQTGAKML
                     ESIPFLAPAIPFVLYHQERFDGTGYPFGLKGESIPIEGRLLSVVDTFDAVVSDRPYRR
                     GRSFEEAITELVRCAGTQFDPHVVELFVK"
BASE COUNT          322 a          452 c          428 g          289 t
ORIGIN      
        1 ctccaacatt ccgataaaat ctacgatacc gctgacccaa cgaccggagc ccgtgtgacc
       61 aaagtacagg taagccgcat cctggtggtg gacgacgaac agtacatctg cgacatcgtc
      121 cgggaggcgc tcgtatccca tccggagtat caaatccaga ccaccgccga tcctcgccgg
      181 gcggtggaaa ttctcaccaa tgagccggtc gatttggtcc tctccgatct gatcatgggc
      241 aagatcaccg gcgtcgaaat cctcgaagtc gcccgaaaat tgcacccgga ctcgatcgtt
      301 attctgatga ccgggcaacc gaccgttgag aatgccgtcg aggtcatcaa gcaaggggcg
      361 tacgactacc tgatcaaacc cttcactctc gagacgctgc gggccaccgt cgaacgcggg
      421 ctggagcgcc agcggctcgc ccgcgagaac gtccacttga aagagcagat ggggctcttc
      481 cgcatcagcg aggcgatggg ttccaccatc cacttggacc agctcttgac cctcatgctc
      541 gatgccatca tcaaagagtt cgacgccgac atggcctcga tccttttggt ggacaaaatc
      601 accggcgaac tgaaactgaa cgccttccgg gggaacacca gcgattcggc cggctcctgg
      661 ttccttttgg gccacgacga agtctgccgc tgggtggtgg acaatggatt gcccaagatg
      721 gtgaacgaaa agcaggaacg ccgccggcgc acctcgccgg ggctcaaaca gaacgtcaaa
      781 tcgttcgtct gctacccgct cttggccaga ggcaaagtgt tgggcgtcct caacgtggtg
      841 cgcaccgaaa aaatggccga attttcccag gggcagttgc agtctttagg catcatcgtc
      901 agcaaggcgg cttcggcgat tgaaagttcc atgctttacg aagaactgca ggacgcctac
      961 ctcgacacca tcatcgtttt agccaacgcc atcgaagcgc gggattccta cacccggctg
     1021 cacacagacc gggtgcgcta cctggccgaa ttgatcgccc gggagttggg ctggccggat
     1081 gcgcggatgc gcgaggtctg gatgggcggc atcctgcacg acgtggggaa aatcgccgtc
     1141 ccggacggga ttttgaacaa gcgcgggccg ctttcgccgg aagagtttac cattatgcgg
     1201 cagcacccgc agaccggggc caagatgctg gaatcgattc cttttctcgc gccggcaatt
     1261 cctttcgtcc tctaccacca ggagcgtttc gacggtaccg gctacccctt cggcctgaaa
     1321 ggcgagagca tcccaattga ggggcgtttg ctctcggtcg ttgacacctt cgacgcggta
     1381 gtgagtgacc ggccctaccg ccggggacgc tcttttgaag aagccataac cgaattagtg
     1441 cgctgtgccg gcacgcagtt cgatccgcac gtcgtcgaac tgttcgtgaa a
//
LOCUS       DBNGZD010000360         2312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1515554, whole genome shotgun sequence.
ACCESSION   DBNGZD010000360 DBNGZD010000000
VERSION     DBNGZD010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1515554"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="AC16GM_09495"
     CDS             <1..274
                     /locus_tag="AC16GM_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HIR2923940.1"
                     /translation="RAHVRTGIGTLLNTGISIGFCSNLYGGGMFAQKSIPPFSWGTSG
                     KIDEYQVDQALLTAEQVQRRREVAFSPAQKRLFLELFEQFRAGKRR"
     gene            418..1416
                     /gene="galT"
                     /locus_tag="AC16GM_09500"
     CDS             418..1416
                     /gene="galT"
                     /locus_tag="AC16GM_09500"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR2923941.1"
                     /translation="MPELRKDPVVGRWVIISTERSRRPTDFTPVPRKSESKNCPFCPG
                     NEAATPPEILAFRPDKSARNTPGWTVRVISNKYPALRIEGDLNREGVGIFDKMNGVGA
                     HEVIIETPDHTKELAHLSPQEVSDVLWAYRERMVDLKNDPRFRYLMIFKNQGEAAGAS
                     LEHAHSQLIALPIVPQQVLEELEGAKRYYDYKERCIFCDGIRQDIADGERVVGQTEHF
                     VALEPFAPRYPFETWILPRFHDAAFERLNPKQFPELAGVLRDILLRLERALANPPFNY
                     ILHTSPVDGDHQKEYHWHFEIMPTVTRVAGFEKGTGFFINPTSPEEAAQYLNEIKI"
     gene            complement(1432..>2312)
                     /locus_tag="AC16GM_09505"
     CDS             complement(1432..>2312)
                     /locus_tag="AC16GM_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2923942.1"
                     /translation="QRLVELNGTSGARTPLQWGKVWVPNSSSWIDDIAVDSYGHLWLA
                     DAINHRLIKISPYDFSTLGTFGSKGTGPNQFLFPCTVGHPEGKEGWADLFVGEEWGPN
                     SGGQYYVMGVDAKDLQTEVSGVNVGGSVGLEFYYEYLLADPAYITENIYQWNPNTSQW
                     VFVARPRNNVTLYSGVTPSSAVVFPGTIGTVDYKIQIYATSTYHNQSGVPVNTDTLEA
                     IVSACSPPLRGDVTLDCVIDISDLTTLVNYVVYSTPMPAGPSMGDANCDGVVDIVDII
                     YLLNYITFGEPVPCPF"
BASE COUNT          512 a          658 c          686 g          456 t
ORIGIN      
        1 gcgtgctcac gtccgcaccg gcatcggcac ccttctgaac accggcatta gcatcggctt
       61 ctgctccaac ctgtacggcg ggggaatgtt tgcgcagaag agtattcccc cattttcctg
      121 gggcacgtcg gggaaaattg acgagtatca ggtcgaccag gcccttttga ccgccgagca
      181 ggtgcagaga cggcgggagg tggcgttcag cccggcgcag aagcggctgt tcctggaact
      241 cttcgaacaa ttccgcgcgg gaaaacgccg ataaataagt gaaaaggaat aaactctggc
      301 cgggcaatca cggcaccctg cggcgagttg atcggaaccg gccgggaatc ggcgtcgtgt
      361 gctgttaccg atacggtccg gtgtgccgcc ctctaaagaa aggcttgaga gcaactgatg
      421 cctgaattac gcaaagatcc ggtggtcggg cggtgggtga tcatctccac cgaaaggagc
      481 cgacgcccca ccgatttcac ccccgttccg cggaaaagcg agagcaagaa ctgtcctttc
      541 tgtcccggga atgaggcggc gacgccgccg gagatcctcg ctttccggcc cgacaagtcg
      601 gcccgcaata ctcccggctg gacggtgcgg gtcatctcca acaagtaccc ggccctgcgg
      661 atcgagggcg acctgaaccg ggaaggggtc ggcatcttcg acaagatgaa cggggtgggc
      721 gcccacgagg taatcatcga aacccccgat cacaccaaag agctggcgca cctctccccg
      781 caggaggttt ccgacgtgct ctgggcctac cgggagcgga tggtcgattt gaagaacgac
      841 ccgcgcttcc gctacctcat gattttcaaa aaccagggag aagcggccgg agccagtctg
      901 gaacacgccc acagtcaatt gatcgccctg cccattgtcc cgcagcaggt gttagaagaa
      961 ttggaaggcg ccaagcgcta ctacgactac aaggagcgct gcattttctg cgacggcatc
     1021 cggcaggata ttgccgatgg cgagcgggtg gtgggtcaga ccgagcactt tgtggcgctc
     1081 gagccgttcg ctccccgcta tcctttcgag acctggattt tgcccagatt tcatgacgcc
     1141 gcctttgaac gcctgaaccc caagcagttc ccggaactgg ccggagtgct gagggacatc
     1201 ctcctgcgcc tggagcgggc cctggccaac ccgccgttta actacatcct ccacaccagc
     1261 ccggtggacg gagatcacca gaaggagtac cactggcact tcgaaatcat gcccacggtg
     1321 acccgggtgg cgggcttcga aaaagggacc ggctttttca tcaaccccac ctcgccggaa
     1381 gaggcggcgc agtatttaaa tgagataaaa atttagggaa cttccgggag gctaaaacgg
     1441 gcaggggacg ggttcaccga aggtgatgta atttaagagg taaatgatat ccactatatc
     1501 gactaccccg tcacagttgg cgtcgcccat gctgggcccg gcgggcatgg gggtgctgta
     1561 gacgacatag ttgacgagcg tggtgagatc ggagatgtcg atgacgcaat cgagggtgac
     1621 gtcgccgcgg agcgggggag agcatgccga gacaattgct tcgagcgtat cggtattgac
     1681 cgggacgccg ctctgattgt ggtaggttga ggttgcatag atttgaattt tgtaatcgac
     1741 tgtgcctatc gtaccgggga acacaactgc tgaggagggt gtaacgccgc tgtagagagt
     1801 tacattattt cgcggacgag caacgaaaac ccactgggac gtgtttgggt tccactggta
     1861 aatattctcc gtaatgtagg ccgggtcggc taataggtat tcatagtaga attccaaccc
     1921 cacgctgcct ccgacgttca cgccgctgac ctcggtttgc aaatcttttg catccacgcc
     1981 catcacgtag tactgcccgc cgctgtttgg tccccactcc tcgcccacga aaaggtcggc
     2041 ccagccctct ttgccctcag gatggcccac ggtgcaaggg aagaggaatt ggttgggacc
     2101 ggtacccttg gagccgaaag tccccagtgt actgaaatcg taaggcgaaa tttttatcag
     2161 ccggtggttt atggcgtcag ccagccagag atgcccgtag gaatcaactg caatgtcatc
     2221 aatccaagat gagctattcg gaacccacac cttcccccac tgcaacgggg ttctagcgcc
     2281 agatgtgccg tttaattcca ccaagcgctg gc
//
LOCUS       DBNGZD010000361         4189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_266476, whole genome shotgun sequence.
ACCESSION   DBNGZD010000361 DBNGZD010000000
VERSION     DBNGZD010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_266476"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..2073)
                     /gene="nuoL"
                     /locus_tag="AC16GM_09510"
     CDS             complement(157..2073)
                     /gene="nuoL"
                     /locus_tag="AC16GM_09510"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR2923943.1"
                     /translation="MLDLIWVVPVAPLASFAILGIFGKDFPKKLVSWLGCGSVGISFL
                     FALGAFSLLLQLPTEQRHVIKHLFTWIASGDFQVKFALQVDPLSAVMMLVVTGVGFLI
                     HVYSIGYMGHDPGYQRFFAYLNLFMFSMLTLVLANNFLLLFVGWEGVGLCSYLLIGFW
                     YERPKAAAAGVKAFVVNRIGDFGFLLGMILIFWTFGSLNFDVVFTLAPLLFNVGAGVI
                     TAITLLLFLGAVGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVARCHVLY
                     QLAPVALEVVAVIGAVTALYAATIGLVQNDIKRVLAYSTISQLGYMFLACGVGAFAAG
                     IFHLMTHAFFKALLFLGAGSVIHALSGEQDMRWMGGLRKSLPRTTWTFLMGTLAIAGV
                     PLFSGFFSKDEILWQAYSSPFGSPVFWLLGVLGAICTAFYMFRLFFLTFSGSNRSPEE
                     VKHHIHESPRSMTLPLTILAVLSVIGGYIGLPQIFGGANRFGQFLEPVFGNGGGEAVA
                     HPASLEWLLLAVSATAIFLSIYLAYSFYIRNPLRAEKVKTAFAWPYQVLFNKYFVDEI
                     YNATIVRPLVEGSQRIYQKFDLAVIDGAANGSAKVVGLFSALYRRVQTGNARNYALSV
                     VLGTIVLIGYLLLR"
     gene            complement(2191..2493)
                     /gene="nuoK"
                     /locus_tag="AC16GM_09515"
     CDS             complement(2191..2493)
                     /gene="nuoK"
                     /locus_tag="AC16GM_09515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR2923944.1"
                     /translation="MIPLSYYLILSGILFAIGVTGVLVCRNAIVIFMSIEIMLNAVNL
                     TLIAFAHYWNQADGQVFVFLVMTVAAAEAAVGLAIILNIFRQKESINIDDVNLMKW"
     gene            complement(2490..3005)
                     /locus_tag="AC16GM_09520"
     CDS             complement(2490..3005)
                     /locus_tag="AC16GM_09520"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR2923945.1"
                     /translation="MELTIFVITGIVALVSAVLVIAQRDPIRSVIFLIVTLCAQAVLY
                     LVLSAPFVAALQVIVYAGAIMVLFLFVVMLLNLGRDQFGKDPRRWQKRVGILFGLILV
                     LQFGLILRKGLFGRPVRAHPVLSTDYGSVAHVGKSLFTDYLYPFEITSVLLLVAIVGT
                     VILAKKKLEET"
     gene            complement(3047..3589)
                     /gene="nuoI"
                     /locus_tag="AC16GM_09525"
     CDS             complement(3047..3589)
                     /gene="nuoI"
                     /locus_tag="AC16GM_09525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR2923946.1"
                     /translation="MQNPVTSTWAILKGLYTTFIHLFRKPITVDYPVKKEPMKPRYRG
                     LHFLERYEDGSERCVCCGLCAAACPADCIYMEPAENEKGERYAKVYEINELRCIFCGY
                     CEEACPEEAIFLGREYEFSADDRNKFIYTKEDLLVPWPRIKPPPEFVRKERRVYSVKD
                     VEADLNKFKPSDRRLTEGTR"
     gene            complement(3617..>4189)
                     /locus_tag="AC16GM_09530"
     CDS             complement(3617..>4189)
                     /locus_tag="AC16GM_09530"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR2923947.1"
                     /translation="WSSNNKYSLLGALRSAAQMISYEVSLGLSVVGILLVAGTTSMLD
                     IVAQQDSFFHWFIFKQPVGFLLYLTCAIAETNRAPFDLPEAESELVAGYQPEYSAMKF
                     GMFYVAEYSNMITVAGIATTLFLGGWQGPFLPPFLWFVLKVFVLLFLYIWIRATLPRF
                     RYDQLMNFGWKVLLPLGLLNIVVTALLTLW"
BASE COUNT         1091 a         1184 c         1152 g          762 t
ORIGIN      
        1 gaaggtaatg gtcgaaactg ccagcgcggt gagtttgagc gcccatttgc tctcccgatt
       61 gatgaaggcc agcaagggca ccccggccag cggcaggaag acaacccagc ttaaaatcgc
      121 gttatccatt acagctcttt cccatcccaa aagacactat cgtaagagca aatagccaat
      181 cagcacaatc gtcccgagca ctacggacag cgcatagttc cgcgcgttgc cggtctgcac
      241 ccggcggtag agcgctgaaa agaggcccac aactttcgca ctgccgttgg cggcaccgtc
      301 aatcaccgcc aggtcaaatt tctggtaaat ccgttgcgac ccctccacca gaggccggac
      361 gatggtggca ttataaatct cgtccacaaa atatttgtta aacaaaacct gatacggcca
      421 ggcaaaggcg gttttaactt tctctgcccg taaaggattg cggatgtaaa acgaataggc
      481 caggtaaatg gaaagaaaaa tggccgtggc cgaaaccgcc aaaaggagcc actccaagga
      541 cgctggatgc gccactgcct ctcccccacc gttgccgaag accggctcta agaattgccc
      601 gaaccggttc gcgccgccga aaatctgcgg caaaccgatg tacccgccga tgaccgacag
      661 cactgccaga atggtgagcg gaagcgtcat agagcgcggc gattcgtgga tatggtgctt
      721 gacctcttcc ggcgagcggt tactcccgga aaaagtgaga aagaataacc ggaacatgta
      781 gaaggcggtg cagattgcgc ccagaacgcc taagagccag aagaccggcg agccgaaggg
      841 actcgagtag gcctgccaga gaatttcatc tttggagaaa aagccggaaa agagtggcac
      901 tccggctatg gcgagcgtcc ccatgaggaa ggtccaggtg gtccttggga gcgatttccg
      961 gagcccaccc atccagcgca tatcctgctc accggaaagt gcgtgaatga ccgatcccgc
     1021 acccaaaaac aaaagcgctt tgaagaaggc gtgggtcatc aggtggaaaa tcccggcggc
     1081 aaaggcgccg accccgcagg ccaaaaacat ataccccaat tgggaaatag tagagtaggc
     1141 caggactcgc ttaatgtcgt tttgcaccaa accgatcgtg gcggcgtaga gtgccgtcac
     1201 cgcaccgatg acggccacaa cttctaatgc caccggggct aactggtaga ggacgtgaca
     1261 gcgcgccacc atgtacactc cggcggtcac catcgtggcg gcgtgaatca gggcggaaac
     1321 tggcgtgggc ccttccatcg catcggggag ccagacgtag agtggaatct gggccgactt
     1381 ccccaccgct ccgaggaaga gcagaagcgt gatggcggtg atgacgcctg caccgacatt
     1441 gaaaagaagc ggggcgaggg tgaagacgac gtcgaaattt aaactcccga aggtccagaa
     1501 aatcaaaatc attccaagga ggaatccgaa atcgccgatc cggttgacga cgaaggcctt
     1561 gacgcccgcg gccgccgcct tgggcctttc gtaccagaag ccgatcaaaa ggtacgagca
     1621 gagccctact ccttcccagc cgacgaagag aagcaggaaa ttattcgcca gaacgagcgt
     1681 gagcatcgag aacatgaaga ggttcaggta ggcaaagaag cgttggtagc cggggtcgtg
     1741 ccccatgtag ccgatggagt agacgtgaat cagaaacccg acgccggtca cgaccagcat
     1801 catcaccgca gagagtgggt cgacctggag ggcaaacttg acctgaaagt caccggatgc
     1861 aatccaggtg aagaggtgtt tgatgacgtg ccgctgctcc gtcggtagtt gcaggagcaa
     1921 tgaaaaggcg cccagcgcaa agaggaaaga aatccccacc gaaccgcacc ctaaccagga
     1981 gactaatttc ttcgggaagt ctttcccgaa gatgccgagg atggcaaaag acgctagcgg
     2041 tgcaaccgga acgacccaga tcagatcaag catatattcc aataacccca ttggtagacc
     2101 ggctcgtcct cgcagagtcg taccgacctg agccggtcgg ggatttcttc ctccgctcca
     2161 tctgtttcat ctgctgcgaa tgacttttct tcaccatttc atcaaattca cgtcgtcgat
     2221 gttgatcgac tctttctgcc ggaagatgtt cagaatgatg gccaacccca ccgccgcctc
     2281 agccgcggct acggtcatca ctaaaaagac gaagacttga ccatcggcct ggttccagta
     2341 gtgtgcaaag gcgatcaaag tcaaattcac ggcgttgagc ataatctcaa tcgacatgaa
     2401 gatgacgatc gcattgcggc agactaaaac gccggtcacg ccgatggcga agaggatgcc
     2461 gctcaagatg aggtagtaac ttaaaggaat catgtttctt ctaacttctt tttggccaga
     2521 atgaccgtgc cgacaatggc cactaagagt aaaacggacg tgatctcaaa cggatagagg
     2581 tagtcggtga aaagcgactt ccccacgtgc gccaccgagc cgtaatcagt ggagagcacc
     2641 ggatgtgccc gtacgggtct gccgaaaagc cccttccgca aaatcaggcc gaattgcagg
     2701 accaaaatca agccaaagag aatccccacc cgtttctgcc agcgccgcgg gtccttgccg
     2761 aactggtcgc ggcctaaatt caaaagcatc accacaaaga ggaataatac catgatcgcc
     2821 ccggcgtaga cgatcacctg cagcgccgcc acaaagggcg cggagagcac taagtaaagc
     2881 actgcctgag cgcagagggt gacgatgagg aaaatgacgc ttctgatcgg atcgcgctgg
     2941 gcaatgacta aaaccgccga gaccaaagcc acaatgcccg tgatgacaaa aattgtgagt
     3001 tccatctctt ctgtcccacc cggtcaaacg acccggacgc cgctccttat cgcgttcctt
     3061 cagtcaaacg cctgtccgac ggcttgaact tgttcaaatc ggcttccacg tctttcaccg
     3121 aatagacccg cctttccttg cgtacaaact ccggcggcgg tttgatgcgc ggccagggca
     3181 ctaagaggtc ttctttggtg taaatgaatt tgttgcggtc gtcggcggag aattcgtact
     3241 cccggcccaa aaagattgcc tcctccgggc aggcctcttc gcagtagccg cagaaaatac
     3301 agcgcagttc gttgatctcg tataccttgg cgtagcgctc gcccttctcg ttttccgccg
     3361 gctccatgta gatgcagtct gccgggcagg ccgccgcaca cagcccgcag caaacgcagc
     3421 gctccgagcc gtcctcatac cgctccaaaa agtgcagtcc ccggtagcgc ggcttcatcg
     3481 gttctttttt gacgggatag tccaccgtaa tcggcttgcg gaagaggtgg atgaaggtgg
     3541 tgtacagccc cttcagaatg gcccacgtcg acgttaccgg attctgcatt ttcagcgcct
     3601 caatttcgac tcgatactac cacagggtta aaagcgcggt gacgacgatg ttcaaaagtc
     3661 ccagaggcaa gagcaccttc cagccgaagt tcatcagctg gtcgtagcgg aagcggggca
     3721 aggtcgcccg aatccagatg tagaggaaca gaagcacaaa gaccttgagc acgaaccaga
     3781 ggaaaggcgg cagaaacggc ccctgccacc cgcccaagaa gagcgtggtg gcaatgcccg
     3841 cgacggtaat catattcgaa tactctgcca catagaacat gccgaatttc atggccgagt
     3901 attcgggttg gtagccggcc actaattccg actcggcctc cggcaggtcg aacggcgccc
     3961 gattggtttc ggcaatggca caggtcagat agagcaaaaa gccgaccggc tgtttgaaga
     4021 tgaaccagtg gaagaaactg tcctgctggg cgacaatatc gagcattgag gtggtgccgg
     4081 ccacgagcaa aatgccgacg acggaaagcc ccagggacac ttcgtacgaa atcatctgcg
     4141 ccgccgaccg caacgccccc aggagcgaat acttgttgtt ggacgacca
//
LOCUS       DBNGZD010000362         4944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1863252, whole genome shotgun sequence.
ACCESSION   DBNGZD010000362 DBNGZD010000000
VERSION     DBNGZD010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1863252"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1258)
                     /locus_tag="AC16GM_09535"
     CDS             complement(347..1258)
                     /locus_tag="AC16GM_09535"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2923948.1"
                     /translation="MSLPRIYKFTFATFGFFCLIQTTSPSLHAESGTSSLVFLKIAPG
                     TRAVAMGEAATALESDLAAAYWNPAATVGLKSHHALFLQNFFILDVNYNYAGFGWGNG
                     KRALGTTLTLSNLSGIQRRGDNPLAEPLGAFELNDAALSFFYAREVREKVSVGGSAKI
                     LYEKIDLASDFTWALDLGGTYRLRPELTVGAAFANLGPKLRLQSESFRLPLRGSLGAA
                     YRWRQVLVTSDFVKFNDGSLKWHLGAEYSFEKYFALRSGWQLGYSDKNFSAGFGLKYQ
                     RFVVDYAFVPFQSDLGQSHRISLEVVF"
     gene            complement(1258..2301)
                     /locus_tag="AC16GM_09540"
     CDS             complement(1258..2301)
                     /locus_tag="AC16GM_09540"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2923949.1"
                     /translation="MTAGEQQRPRQRRGSQGPVARKRQAIMTLVVGLLLIFGHKHPRA
                     ATGDGGYAAPFLQLGLGARAVAMGGAFSAIADDGTAPLWNAAASAWLVKRQFSATYRK
                     MFLDRSTGYVSILFPVRHEAAFGANWRFAGTDDIFGRDDVGDITEKIKNFENQFSVNF
                     ARRFTRALALGVNIKYLQQNLANINAFSVGFDLAAHFIPNRDLKLGKTVIYMRSLRIG
                     LAVENLGLKYPWNSAEYYSRFGQLGSSVTDNVPVNYKAGISYRFKRTPALLAVDVEKN
                     SRQRARSHFGTEYTAFRILALRAGVNRGDPTFGLGVRQPFRKAELGLDYAFVTRPVAG
                     ADPDHLLTLQIAF"
     gene            complement(2298..4760)
                     /locus_tag="AC16GM_09545"
     CDS             complement(2298..4760)
                     /locus_tag="AC16GM_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923950.1"
                     /translation="MPEVLLGVGIFLLALSPSLFGVGLFSFDLQGTPIPRLRANSAVD
                     IVRKGTPDTIWVGTGRGVSFTADSGRTWFTFDHTNGLSADEISALATDGIRLWVGTSH
                     SVVFGNQAVSFGDGFSVTTDNGFSWQSPQPQQAVGGAKVPFDIAFSNLPADTTIWSVA
                     FCGGLFRSTDFGTNWTNVYPDTTYKGTCELGQDNNPRDLNNRFFSVVADTNRPDTAVI
                     WAGSANGVNKFTYIRKRSRLAGNRIGALGFADSLLWLGTEVGLSTTNPFDSLHPFRTN
                     TTTPQLQNVGISAVFADSQKIIVATASAESLKAGIDLLISRDSGRTWQLRGDSLMVGL
                     NRVVTSIATADSILFVTAKSGGLLRSFNSGQSWQRIDSTGRSFFSVATQKIQPDTLVV
                     WAGGDSGLFKFVFGGAVDTLDTRTHYGVSDAAFLGNRVTLVKVFVDSGLVWAGVEPDS
                     LPQRFGLHRTRNDGANWDTSFVDQQAHDLAFAPYGFWLGTSGGLWRSINQGILNSWRQ
                     VTVKDTTTRDSLPGVEVFSLIPVANRLWVGTEVGLATTDSGAQFSIFRTGKGEADRIQ
                     RFTSASHGLSGNFITAMGIQRFSSQKILWAATQFAGGVETNALSFTKDDGKTWQVTLP
                     NVRAWNFSFADSVVWIATSQGLYHSFNLGQTWKRDTLVDFTDGTSISDLSEVFGVLAF
                     TTEVWVATDNGLAHSTDKGNTWRVIRAFKGIAPPPAPQESLTYAAPSPFSPLRSPGFC
                     SIHYRPEKTTQVTIRVYDFALNLVKTVTENQPRLAGVQYDDLDLWDGKNEKNDVVATG
                     VYFYQIDFADGQDLWGKVVVLK"
BASE COUNT         1095 a         1358 c         1521 g          970 t
ORIGIN      
        1 ggcgtacatc cttttggtgg gggcggaaat cggcgtgcac tttgaaaacg tgcgccagcg
       61 ctaccggatt ttcgcttttt ttcgcaaagc cttcgggcaa gggttttcat agacgtaaca
      121 aatggcgacg gcccgtaggg ctccccgcta caaaagcggc agttccgagg ctggaagcct
      181 cggctatgga ggtaaagatc tcttctccca taggtcaggc ttccagcctg actaaatttc
      241 cgaaccggga aaacgccctc ggcgtccgcc cgagcgtggt ccaaacaacg acggctcttt
      301 ccgagccgcg gaggttaaaa acctcggcta cgaaattagg gaaatttcaa aacaccactt
      361 cgagggaaat gcggtggctc tgacccaagt cggactggaa ggggacgaag gcgtaatcga
      421 cgacgaagcg ctggtacttg aggccgaaac cggcgctgaa atttttgtcc gagtagccca
      481 actgccagcc gctgcgcaag gcgaaatatt tttcaaaact atattcggcg cccagatgcc
      541 atttgagact gccgtcgtta aacttcacga agtcactcgt caccagtacc tgccgccagc
      601 ggtaggcggc acccagggag ccgcgcaggg gtaagcggaa cgattcgctc tgcagacgaa
      661 gtttgggtcc taagttggca aaagcggcgc cgacggttaa ttcggggcgc agccgatagg
      721 ttccgccgag gtccaaggcc caggtgaagt cgctggcgag atcgattttt tcgtacaaaa
      781 tcttggcgga acccccgaca gaaacttttt ctctcacttc ccgggcgtag aaaaacgaga
      841 gcgccgcgtc gttcaattca aaagcgccca agggttcggc caaagggttg tcgccgcgcc
      901 tttggatgcc ggagaggttg ctcaaggtga gcgtcgtgcc cagcgctctc tttccgtttc
      961 cccagccgaa gccggcgtag ttgtagttca cgtcgaggat gaaaaagttt tgcaaaaaaa
     1021 gggcgtggtg gcttttgagc cccactgtcg ccgccggatt ccagtaggcg gcagccaaat
     1081 cactctccag ggctgtggcc gcttcaccca tcgccaccgc gcgggtgccg ggcgcgattt
     1141 tcaaaaagac gaggctggaa gtaccgcttt cggcgtgtag cgaaggagaa gttgtctgga
     1201 tgagacaaaa aaacccaaac gttgcgaaag tgaacttgta aatccgaggc aatgacatca
     1261 gaaagcgatt tgcagagtca agaggtggtc aggatcagca cctgcaacgg gacgggtcac
     1321 gaaagcgtaa tccaagccga gctcggcttt ccggaaaggt tgtcgcactc ccaagccgaa
     1381 agtgggatca ccccggttca cgccggcccg caacgccaaa atccggaagg cggtgtattc
     1441 ggtgccgaag tggctccgtg cgcgctggcg gctgtttttt tccacatcga cggccagaag
     1501 tgccggggtg cgtttgaagc ggtaggaaat cccggctttg tagtttaccg gcacgttgtc
     1561 ggtcaccgaa ctgcccagct ggccgaagcg ggagtaatac tccgccgaat tccaggggta
     1621 cttgaggccc aggttctcga cggccagtcc aatgcgcagg gaacgcatgt aaatgacggt
     1681 cttccccagt ttcagatcgc ggttcggaat gaagtgggcg gccaggtcaa agccgaccga
     1741 gaaggcgttg atgttggcta agttctgctg aaggtatttg atgttcaccc ctaaggccag
     1801 cgcgcgggtg aaacggcggg cgaagttgac ggagaactgg ttttcgaaat tcttgatttt
     1861 ttcggtgatg tcccccacgt cgtcgcggcc gaagatgtcg tcggtgccgg caaagcgcca
     1921 gttggcgcca aaggccgcct cgtggcgcac cggaaagagg atgctgacgt agccggtgga
     1981 gcgatccaaa aacatctttc gataagtggc ggaaaactgg cgcttgacca accaggcgga
     2041 ggcagccgca ttccacaggg gagcggtgcc gtcatccgct atggcagaga aagccccgcc
     2101 catggccaca gcacgggcgc ccaggcccag ctgcaaaaaa ggcgccgcat agccgccgtc
     2161 accggttgcc gcccgcgggt gtttatgtcc aaagatgagt agcagcccta ccaccaacgt
     2221 catgatggct tgtcttttcc tggctactgg cccctggctc ccgcgccgct ggcggggcct
     2281 ctgctgctcg cccgctgtca tttcagcacc accactttcc cccagaggtc ctggccgtcg
     2341 gcaaaatcga tctgataaaa atagacgccc gtggcgacga cgtcgttctt ttcgtttttc
     2401 ccgtcccaga gatcaagatc atcatactgc acaccggcca ggcgtggctg gttctcggtt
     2461 acggttttga ccagattcag ggcaaagtcg tagacccgga tggtgacctg cgtggttttc
     2521 tccggccggt agtgaatgga gcagaaaccg ggactgcgca ggggtgaaaa gggagagggg
     2581 gcggcgtagg tgagcgactc ctgcggcgcc gggggtgggg cgatgccctt gaaggcgcgg
     2641 atgacccgcc aggtattgcc tttgtcagtt gaatgagcca ggccgttatc ggtggctacc
     2701 cacacctcag tagtgaaagc caggacgcca aatacttccg acaaatcgct gatggaggtg
     2761 ccgtcggtga aatcgaccaa ggtgtcccgc ttccaggtct ggcccagatt gaaagaatga
     2821 tacaaccctt ggcttgtggc aatccagacg accgagtcgg caaacgagaa attccaggcc
     2881 cgcacgttcg ggagcgtcac ctgccaggtc tttccgtcgt ccttggtaaa cgagagggcg
     2941 ttcgtttcca ctccgcccgc aaactgagtt gcggcccaga gaattttttg actgctgaag
     3001 cgctgaatgc ccatggcagt gatgaagttc cccgagaggc cgtggctcgc tgaggtaaag
     3061 cgctgaattc tgtccgcctc tccctttcca gtacggaaaa tggaaaactg tgcgccgctg
     3121 tcggtagttg ccaaacccac ttcggttccg acccagagcc ggttggctac ggggattaaa
     3181 gagaaaacct cgactccagg gagggaatca cgggtagtgg tatctttgac tgtcacctgt
     3241 ctccaggagt tcaaaattcc ctgattgatg ctgcgccaga ggccgccgga agtgcccagc
     3301 cagaagccat agggagcaaa ggccagatcg tgtgcctgtt gatccacaaa actggtgtcc
     3361 caatttgcgc catcgttgcg cgttcggtgg aggccaaagc gctgggggag cgagtcgggc
     3421 tcgacaccgg cccagaccaa tcccgaatcg acaaaaactt tcacgagggt cacccggttg
     3481 cccagaaagg cggcatcact gactccgtag tgggtgcggg tgtccagcgt atcgaccgcg
     3541 ccgccgaaga cgaacttgaa cagcccgctg tcgccccccg cccaaaccac cagcgtatcg
     3601 ggttgaattt tttgtgtggc gacagagaag aaactccggc cggtgctgtc aatccgttgc
     3661 caggactggc cggaattgaa actgcgcagg agtccgccgg acttcgctgt gacaaagaga
     3721 atcgaatcag cggttgcaat tgaggtgacg acgcggttga ggccgaccat caaggaatcg
     3781 cctctcagtt gccaggtgcg gccagagtca cgggaaatga gcaaatcgat tccggctttc
     3841 agcgactcag ctgacgccgt ggcgacaatg attttctgcg agtcggcaaa aacggcggag
     3901 atgccgacgt tttgcagttg cggtgtagtc gtattcgttc ggaaagggtg gagtgagtca
     3961 aatggattgg ttgtcgaaag tcccacctca gttcccagcc agagcaaact gtcggcaaag
     4021 cctagggcac cgatacggtt gccggccagc cggctgcgct tgcggatgta ggtgaacttg
     4081 ttcacgccat tagcggagcc ggcccagatc accgccgtgt cgggccggtt cgtgtcggcc
     4141 acgaccgaga agaaacggtt gttcaggtcg cgggggttgt tgtcttggcc taactcgcag
     4201 gttcccttgt aggtggtatc cggatagaca ttcgtccagt tggtgccaaa atcggtggag
     4261 cggaacaagc cgccgcaaaa agcgactgac caaattgtgg tatccgccgg cagattggaa
     4321 aaggcaatgt cgaatggcac ttttgctccc cccacggcct gctgcggttg cggcgattgc
     4381 cacgagaagc cattgtcggt ggtaacgcta aagccgtcgc caaaagatac ggcctgattg
     4441 ccgaaaacaa ctgagtgcga ggttcccacc cataaccgga tgccatcggt cgccagggcg
     4501 gagatttcgt cggcgctcaa gccgttggtg tggtcaaagg tgaaccaggt ccggccgctg
     4561 tcggcggtga aactgacccc ccgaccagtg cccacccaga tggtgtccgg agttcctttg
     4621 cggacgatgt ccactgccga gttggcccgg agccggggga tgggcgtgcc ctgcaggtcg
     4681 aaggagaaaa ggccgacgcc gaaaagtgaa ggagagagag ccaaaagaaa aatcccgacg
     4741 ccgagcagaa cttccggcaa aaaccgtcgg gacctgcggt agaagcaccg aggcggtttt
     4801 cccacgcgag aaagataccg cgtaaggggg attttgtcaa taaaaagcag gttcatagcg
     4861 gtagtcgggc cggaacggcc cgtatgggct cgtcctcggt acgagttgta tcgactcgag
     4921 cccgacttgg tttcgaccgg ctcg
//
LOCUS       DBNGZD010000363        11638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_196255, whole genome shotgun sequence.
ACCESSION   DBNGZD010000363 DBNGZD010000000
VERSION     DBNGZD010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_196255"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="AC16GM_09550"
     CDS             <1..1156
                     /locus_tag="AC16GM_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2923951.1"
                     /translation="EAERLQAPEEVPHPSAWLPRVAAYGLGTAGLSALLAVWLWPGFF
                     WIVAGAGSIGGIAAGVFFLQWKIRRDRIMAWELRKSRLAEVERETLAAQNALRTLLMK
                     YRLPSAAALREQYEKTRDVERQIESEMVRYESLLDGKTQKEIENRLQEVIGELNTLKK
                     VKQELGARTVTAEEVAELEATVATLADEEANLTTAAGALRERLAAAGMGSEELTRLQE
                     ERLAVDRSLASMKFRQKLYTLTAEGLLQAQETVQSTVVQVLEHEVAWAVEQLSLGRYS
                     KVRLDPQVFRFDLYSAERKDWVASAEPLSHSAAGQVNLAIRLALLKVLSEREKPVVIL
                     DEPFAAFDAEREKQALALLEKLAEEYQIFLFTCRPVPDRWPARGSLVRLA"
     gene            1212..1727
                     /locus_tag="AC16GM_09555"
     CDS             1212..1727
                     /locus_tag="AC16GM_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR2923952.1"
                     /translation="MERIWAPWRKAFILKTREAGCLFCELAAEREDKKSLVVFRGRRA
                     YVVLNRYPYTNGHLMVVPYRHVAELGGMTPGEEKELWALARRSVQLLRQAYQPQGFNL
                     GLNLGRAAGAGVVDHLHLHVVPRWAGDNNFMPVIGNTKIHSLSLDDTFRTLAPGFRQK
                     RLPSQKTRKQR"
     gene            1724..3313
                     /locus_tag="AC16GM_09560"
     CDS             1724..3313
                     /locus_tag="AC16GM_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012550.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR2923953.1"
                     /translation="MKIPKKKDLLRLQAELKSDRKIAEALGGVPQHLIGYWRRKKNIP
                     LRGGPRFTRKEIEFLWEKHGNDRKASSELGISRAAFYRWRKFYGLHEKPKWVRLQKQQ
                     LELHFPRRLILKSGALPQTLVQKIVGRKLAPEAAGTRPQLFLETSVAVAPDRLICHAR
                     QAEALVELPWPDGSAPILERLGFDFSGYDPGLFTGAPLRLWEQAQALKNSVNSVEKEK
                     PNRRLWEAEDRCAPGLLLAGTDRSVAGCGALGSWGIQVDSWDVGGYLTCGRIWTELPA
                     AVRVRLDGRLPESCTVHDLSFRFIHEVYQRDLAGMVIELTGEAVRRLKIPARLLFCAI
                     AAEAGITALFPYDEVTCALGAEKGGAASDPFFPDRQALYAQRIELNMDHPHFWAAGTA
                     GAHQVQELSTLVGTKIAGALVGGCGTGRIEDLKLAAALTEGKELASGAELFVVPDSAP
                     VYLEAIRKGYVKVLVEAGAQILPPGDHWTFATAERMQTLGLLATSPVLLRSELPRPER
                     LWCVSPAAAVRSAVAGEIVLP"
     gene            3381..4154
                     /locus_tag="AC16GM_09565"
     CDS             3381..4154
                     /locus_tag="AC16GM_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR2923954.1"
                     /translation="MRYSTAGHLPNFLVTHAFWRRMGWILAILAGLWLFFIAFDRVLL
                     PFVVRHGQDREVPNITRAPFDAAEKILTERQLGFAIVGREYHPDEPEGIVISQTPLPG
                     AVVKKGRVVKVFISRGGETAVVPALVGLTLRQAQIALLDAGLDLGAIGDTTVDTLPVG
                     QVVASQPPAGANLRLGIPVKLFVNQAAQVDSLVVPHLVGRSLAEARLVLGDLGLALGQ
                     IRYEKNDKLLPGTVTEQAPEAGTVVGVPAIVELTVSKTD"
     gene            4162..4863
                     /gene="rpe"
                     /locus_tag="AC16GM_09570"
     CDS             4162..4863
                     /gene="rpe"
                     /locus_tag="AC16GM_09570"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR2923955.1"
                     /translation="MRRQAGHAVKRFYSSQRRQVLVAPSLLACDIRHLADEINKAEAA
                     GADLFHLDIMDGHFVPNLTFGPPVVAAVRQVTRLPLDVHLMITDPHRYLRDYREAGAD
                     FITVHQEAVLHLHRVVAAVKETGACAGVALNPATPFNLLDDILAELDLVLVMSVNPGF
                     SGQKFIPGALRKIHQARERKDTRGHHYLIEIDGGMNGQTAPGAVAAGAEVIVAGNGIF
                     SHRNYRRAIGSLRGR"
     gene            4951..6285
                     /gene="ffh"
                     /locus_tag="AC16GM_09575"
     CDS             4951..6285
                     /gene="ffh"
                     /locus_tag="AC16GM_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR2923956.1"
                     /translation="MFAQLTEKLESVFKSLRGRGKLTEANIREALVLVRRALLEADVH
                     YRVVKEFLAAVEAKALGREVLESITPGQQVVKIVRDELVHLLGDRAHPLATAPHPPTV
                     ILVVGLQGSGKTTFCGKLAKRLAAQNRPVLLAAADRQRPAAVVQLQSLGRTVGVPVFT
                     GAGTPVEIAQAAVAQAESQLQDYVIIDTAGRLHVDAELLAELAEMKKTAHPHEVLFVA
                     DALTGQAAIEMAQKFQSAVHLDGIVLTKFDGDARGGAALSIRAVVGAPIKFLGVGEKP
                     EDLEVFHPDRLASRILGFGDVVSLVEKVEQAVSAEEARETEEKILAERFTLEDFLSQL
                     QQMKKMGPVESLLGLLPGVNRKALQGAAVDEKALGKVEAMIYSMTRAERSHPEIIDGS
                     RRRRVAAGSGTMPSDVNGLLKQFLAMQKMVRQFKHTAGRRGRGSILSGLGMR"
     gene            6312..6557
                     /gene="rpsP"
                     /locus_tag="AC16GM_09580"
                     /pseudo
     CDS             6312..6557
                     /gene="rpsP"
                     /locus_tag="AC16GM_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722284.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            6764..6994
                     /locus_tag="AC16GM_09585"
     CDS             6764..6994
                     /locus_tag="AC16GM_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR2923957.1"
                     /translation="MKEFVEKIVRSLVEHPDQVQVTEVEAEKTVVLELRVAPDDIGKV
                     IGKHGQNAKALRTLLTAVSARRGKRAVLEILE"
     gene            7018..7764
                     /gene="trmD"
                     /locus_tag="AC16GM_09590"
     CDS             7018..7764
                     /gene="trmD"
                     /locus_tag="AC16GM_09590"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR2923958.1"
                     /translation="MRIDILTIFPEIFGGYLGSSLIAKAQAKGLVTIRPVDLRQFATG
                     KHREVDDAPYGGGAGMVFMVEPVYRAVQDLVAVAGPHVSRRVVLLSPQGVQFAQTTAQ
                     EFAKTEHLILICGRYKGVDERVKALVVTDELSIGDYVLNGGEAAAMVVVDAVFRLLPG
                     ALGKSDSAETDSVASGLLEYPVYTRPEEFLGQRVPEILLSGHHEEVRKWRRTQALRRT
                     KERRPDLFGRFTLTDEDRELLQEKDRDKEI"
     gene            7812..8204
                     /gene="rplS"
                     /locus_tag="AC16GM_09595"
     CDS             7812..8204
                     /gene="rplS"
                     /locus_tag="AC16GM_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR2923959.1"
                     /translation="MDLLKAAEAKFLKERKVAFGPGDTIKVHVKIKEGDKERTQMFQG
                     TCIQKRGTGTGASFTVRKMTEGVGVERVFPLHSPFVTKIDLVKRGNVHRAKLFYLRQL
                     RGKSARIEEKKKEEEPTPLRARAEAAVA"
     gene            8258..8929
                     /locus_tag="AC16GM_09600"
     CDS             8258..8929
                     /locus_tag="AC16GM_09600"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR2923960.1"
                     /translation="MPQGEATGEIPCSGGVDLFAFERVLWESGWELVAGVDEAGRGPL
                     AGPVVACAVVFPKDCPSIPVNDSKKLSAAERLALIGPIGRTALSVGVAVVQQEAIDRF
                     NILQATFSAMRQALTRLRSKPDLVIVDGRQRIPGLAFPQFPLVKGDARSASVAAASIV
                     AKVVRDRIMEEMDETYPAYGFARHKGYATPSHRAALLRFGPSPVHRASFLKKLFAPVQ
                     ERLAL"
     gene            8972..9337
                     /locus_tag="AC16GM_09605"
     CDS             8972..9337
                     /locus_tag="AC16GM_09605"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR2923961.1"
                     /translation="MKKSKTQFGAEVEQTVAQYLTNCGWQILERNWRSGHREIDLIAR
                     DGRALVFVEVKGARSLSFGQPEERVDFAKRRHLNDAALAYLQAHEPEASEYRWDVVTV
                     TFAKGAPTINHLRNAFTAD"
     gene            complement(9324..9692)
                     /locus_tag="AC16GM_09610"
     CDS             complement(9324..9692)
                     /locus_tag="AC16GM_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923962.1"
                     /translation="MSPSTHKILLVLFFAILFVAILQLRDTPRQTRLSENEFIALTAD
                     LAVAQSRYSTNPGLWQKEIENILARSHVTREQIEKFRQKVDRSPEKWAHIWNEVARKL
                     QEKETAAGHPKTASPPVNPP"
     gene            9803..10918
                     /gene="ispG"
                     /locus_tag="AC16GM_09615"
                     /gene_synonym="gcpE"
     CDS             9803..10918
                     /gene="ispG"
                     /locus_tag="AC16GM_09615"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR2923963.1"
                     /translation="MDSPKKTNGNLPRSRTRQVTVGKIKLGGGAPVVVQSMCTTDTRD
                     VQATLSQIRQLAEADCELVRLAVLDEKAALALKELVPHAPVPIIADIHFNYKLALAAV
                     EAGVQKLRLNPGNVGEDWKVREVVKRVKERGIPIRIGANSGSLPKDIIEKYGKPTPEG
                     IVEAALRHVAILEEMDFHDIVVSLKSSEVRITVDAYRLMAETAPQYPLHIGITEAGTA
                     WAGTIKSSVGLGILLADGIGDTMRISLAADPVEEVKVAYEILKDLEIREHGVTLIACP
                     TCGRCEIDFMPLVQKVEVELRKIREPIRVSVMGCVVNGPGESREADVGVSAGRGLGLI
                     MRKDKVVRKVKEEEIFNALMEEVQKVLAERRTQAVAT"
     gene            10940..11401
                     /locus_tag="AC16GM_09620"
     CDS             10940..11401
                     /locus_tag="AC16GM_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923964.1"
                     /translation="MKQKSNGQQVEFIGTKLLEAELVRNGFEVAYPIRDRGIDFIIYQ
                     DNHGKPFSAVPIQVKAASGERFILDRKYERMKGLILAYVWGIDKKPRYFLLTYREALS
                     LLGRARKTNSWQKKGIYVYPHVPHRLKEKLHQYYEDRWTWLRKLLRTKTQK"
     gene            11414..>11638
                     /locus_tag="AC16GM_09625"
     CDS             11414..>11638
                     /locus_tag="AC16GM_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2923965.1"
                     /translation="MPNPVVHWEIAVKNFDKAKAFYSGLFGWKVDSNNPMNYGIVDTA
                     AGGINGGIFQAQKNMPQNYVTFYIQVDDLQK"
BASE COUNT         2378 a         3254 c         3797 g         2209 t
ORIGIN      
        1 ggaagccgaa cggctccagg caccggagga agtgccgcac ccttccgcct ggcttccgcg
       61 ggtggcggcc tacgggctgg gaaccgcggg gctgtccgcg cttctggccg tctggctctg
      121 gccgggtttc ttctggatcg tcgccggggc ggggtcgatc ggcggcatcg ctgcgggagt
      181 tttcttcttg cagtggaaaa tccgccgcga ccggattatg gcctgggaac tccgcaagag
      241 ccggctggcc gaagtggaac gggaaacgct ggcggcccag aatgcgctgc gcacactctt
      301 gatgaagtac cggttgccgt cggcggccgc cctgcgggaa cagtacgaaa aaacgcggga
      361 cgtggagcgc cagattgaaa gcgaaatggt gcgctacgaa agtttactgg acgggaagac
      421 gcagaaggag attgaaaacc ggctccagga ggttattgga gaactgaaca cgctgaaaaa
      481 ggtgaagcag gaactggggg cccggaccgt tacggcggaa gaagtggcgg agctggaggc
      541 caccgtcgcc accctggctg acgaagaggc caatctcacg acggcggccg gcgccttacg
      601 ggaacgattg gctgccgccg ggatgggcag tgaggaactc acccggttgc aggaagagcg
      661 tctggcggtc gaccggtccc tcgcgtcaat gaaattccgg caaaaactct ataccctcac
      721 ggccgaaggc ctgctccagg cccaagagac ggtgcagagc accgtggtgc aggttctgga
      781 acatgaagtg gcctgggcgg tggagcagtt gtctctgggc cggtattcaa aggtgcgcct
      841 cgatccgcag gtcttccggt ttgacctgta ctcggccgag cgcaaagact gggtcgcatc
      901 cgctgagccg ctttcgcaca gcgcggcggg acaggtcaac ttagccatcc ggctggcctt
      961 gctcaaagta ctctccgagc gcgagaagcc ggtggtgatt ttggacgaac cgttcgccgc
     1021 ctttgatgcg gaacgagaaa agcaggccct ggccctgctg gagaaacttg ccgaagagta
     1081 tcagattttc ctttttacct gccgaccggt gccggaccgg tggcccgccc gaggcagtct
     1141 ggtgagactg gcctgaatcc tgcgggggcg ggattggcgg gtcgaccgca gcgggatata
     1201 ttgccgggcc aatggaacgg atctgggcac cctggcgcaa ggcattcatt ctcaaaacca
     1261 gagaagccgg ttgcctcttc tgcgaactgg cggccgagcg ggaggacaaa aagagtctcg
     1321 tcgtgttccg gggccggcgg gcctacgtcg ttctgaaccg ctacccctac accaacggtc
     1381 acctgatggt ggtgccgtat cgccatgtgg ccgagctggg agggatgacc cccggcgaag
     1441 aaaaagagct ctgggcactg gcccggcgga gtgtgcagct tttgcggcag gcctaccaac
     1501 cccagggatt taacctcggg ctgaatctgg gccgggctgc cggcgccggc gtggtcgacc
     1561 acctgcacct gcacgtggtg ccgcggtggg ccggggacaa caatttcatg ccggtcatcg
     1621 ggaacaccaa aatccactcc ctttccctgg acgatacctt ccgcaccctg gcgcctggtt
     1681 ttcggcaaaa acgtttaccg tcgcagaaga cccgtaagca gcgatgaaga tccccaagaa
     1741 aaaagacctc ctccggctgc aggccgaact gaaaagcgac cgcaagatcg ctgaggccct
     1801 gggcggcgtg ccgcagcatt tgatcggcta ctggcggcgg aaaaagaaca ttcccctgcg
     1861 cggcggaccc cgctttaccc ggaaggaaat tgagttcctc tgggaaaagc acgggaacga
     1921 ccggaaagcc agtagcgaac tgggtatttc gcgggccgca ttctatcgct ggcgcaaatt
     1981 ttacggcctg catgaaaaac cgaagtgggt ccggctgcaa aaacagcaac tcgaactgca
     2041 cttcccgcgg cggttgatcc tgaaatccgg agcgcttccc caaacactgg tgcaaaaaat
     2101 tgtcgggcga aagctggcgc cggaagcagc ggggacccgg ccgcaattgt ttctggaaac
     2161 cagtgtcgcc gtggccccgg accggctgat ctgccatgca cggcaagccg aggcgctggt
     2221 ggaactcccc tggccggacg ggtcggctcc catcctcgag cgccttggtt tcgatttctc
     2281 gggatacgac ccggggctct tcaccggtgc gcccttgcgc ttgtgggagc aagctcaagc
     2341 actcaaaaac agcgtcaatt cagtggaaaa agaaaagcca aaccgccgcc tctgggaagc
     2401 ggaagaccgg tgcgctccgg gtctcctctt ggctggaact gaccggagtg tcgccggctg
     2461 cggggctttg gggagttggg gaattcaggt tgattcttgg gacgtcgggg gatacctcac
     2521 ctgcggccgg atatggactg aactgcccgc tgcagtacgg gtgcggctgg acggtcgctt
     2581 gccggagagc tgcacggtgc acgacctgag tttccgcttc atccacgaag tgtaccagcg
     2641 cgacctagcg gggatggtga ttgagctcac cggagaggcg gtgcggcggc tgaaaattcc
     2701 cgctcggctt ttgttctgtg ccatagccgc tgaggccggg attaccgctc tttttccgta
     2761 tgacgaggtt acctgcgctc tcggagccga aaaagggggg gcggccagtg acccgttttt
     2821 cccggacagg caggcgttgt acgcccagag aattgagctc aatatggacc acccccactt
     2881 ctgggccgcg ggaacagccg gtgcgcacca ggtacaggag ttgtccacac tggtggggac
     2941 gaaaatagcg ggagcgctcg tcggcggttg cggcaccggc cggatagaag atctgaaact
     3001 ggcggccgct cttacggagg gcaaagaact ggcctccggg gcagagcttt tcgtggtgcc
     3061 cgattcggct ccggtttact tagaggcgat ccgcaaaggg tacgtcaaag tgctggtcga
     3121 ggcaggggct caaatactgc cccccgggga tcactggact tttgccactg ccgagcggat
     3181 gcaaacgttg ggtctcctcg ccaccagtcc ggtgctgctc cgctccgagc tcccacgtcc
     3241 ggaacggctc tggtgcgtga gcccggcggc ggcggtccgg tcggccgtag ccggcgagat
     3301 cgtgttgccg taacctttcg ggtggtccgg ggcattgctc aaacgggcaa tgtcggccat
     3361 attctcgggc gaaagggcga gtgcggtatt caacggctgg acatcttccg aactttctgg
     3421 tcacgcatgc cttctggcgg cggatgggct ggattcttgc cattctggcg ggtctgtggc
     3481 ttttctttat cgcctttgac cgagttcttt tgccctttgt cgttcggcac gggcaggatc
     3541 gggaagtgcc gaatatcacc cgggctccct ttgacgcggc ggaaaaaatt ctcaccgaac
     3601 ggcaactggg gtttgccatt gtgggccggg aataccatcc cgatgagccg gaggggatcg
     3661 tcatttcgca aacccccctg cccggggcgg tggtgaaaaa agggcgcgtg gtcaaggttt
     3721 tcatttcccg cggcggcgaa actgcggtcg tcccggccct ggtggggctc accctgcgcc
     3781 aggcgcaaat tgcgctttta gatgcgggac tggacctggg cgccatcggg gacaccacgg
     3841 tggacaccct tccggtgggc caggtggtgg cttcgcagcc tccggccggg gccaacctgc
     3901 gcctgggaat tccggtgaaa ttgttcgtca accaggcggc ccaggtcgac tcgctggtgg
     3961 tgccgcacct ggtggggcgg agtttggcgg aagctcgttt ggtcctgggt gatctggggc
     4021 tcgcgctggg acagatccgc tacgagaaaa atgataaact gttacccggg acggtcaccg
     4081 agcaggcgcc cgaagcgggc accgtcgtgg gggtacctgc catcgtggaa ttgacagtga
     4141 gcaaaacgga ttgagctatc cgtgagacgc caagccgggc acgcggtcaa gcggttttac
     4201 tccagtcagc ggcgacaggt tctggtggcg ccttcgcttt tggcctgcga catccgccac
     4261 ttggccgacg agatcaacaa agcggaggcg gccggggcgg acctcttcca cctcgatatc
     4321 atggacggcc acttcgtccc caacctgacc ttcggccccc ccgtggtggc ggcggtacgc
     4381 caggtgaccc gcctcccttt ggacgtccac ctgatgatca ccgatcccca ccgatacctg
     4441 cgggattacc gggaagcggg ggccgatttc atcacggtac atcaggaagc ggtgctgcac
     4501 ctccaccggg tggtggccgc cgtcaaggaa accggtgcct gcgccggtgt ggcgctcaat
     4561 ccggcgaccc ccttcaatct gctggacgac atcctggctg aactcgattt ggtattggtg
     4621 atgtcggtga accccggttt cagcggccag aaatttattc ccggtgccct gcgtaagatt
     4681 catcaagccc gggagcgtaa ggacactcgg ggacatcact acctcatcga gatcgacggc
     4741 gggatgaacg gccagaccgc tccgggagcg gttgccgccg gggctgaggt catcgttgcg
     4801 gggaacggaa ttttcagcca ccgtaattac cgtcgggcga tcggctcact ccgcggccgg
     4861 tagtccagtt gcacattctc aaaaatttgt tataataaaa agttctccct ccggttaaga
     4921 atgcgtcctc cggctggtga gcggaaaaat atgtttgcac agttgaccga aaaacttgag
     4981 tcggttttca aatccctgcg cggccgcggc aaactgaccg aggcgaatat ccgcgaggct
     5041 ttggtgctgg tccgccgggc gcttttggag gccgacgtcc actaccgggt ggtcaaagag
     5101 tttctggctg cggtggaagc caaggcgctg ggccgggaag tgctggaaag catcaccccc
     5161 gggcagcagg tggtgaaaat cgtccgcgac gaattggtcc acctcttagg cgaccgggct
     5221 caccccctgg cgaccgcgcc gcacccgccg acggtgatct tagtggtcgg cttgcagggc
     5281 tccggcaaaa ccaccttctg cgggaaactg gcgaaacgtc ttgcggcgca aaaccggccg
     5341 gttctcttag cagcggccga tcggcagcga ccggctgcgg tcgtacagtt acaatccctc
     5401 ggccggacgg tgggggtccc ggtttttacc ggcgccggaa cgcctgtgga aattgcgcag
     5461 gcagccgtgg cgcaggccga aagccagctc caggactacg tcatcatcga caccgccgga
     5521 cggctgcatg tcgatgccga acttctggcg gaactggcgg aaatgaaaaa aacggcacac
     5581 ccccacgaag tgctcttcgt ggcggacgct ctgaccggtc aggcagcaat cgagatggcg
     5641 caaaaatttc agtccgccgt gcatctggac ggcatcgtgc tcaccaaatt cgacggtgat
     5701 gcccgcggcg gggcggcgct ttcgatccgt gccgtcgtcg gcgcgccgat taagttcttg
     5761 ggcgtcgggg aaaagcccga ggatctggag gttttccatc ccgaccggct ggcgtcccgc
     5821 attctcggtt tcggcgacgt cgtttcgctg gtggagaaag tagagcaggc ggtctcggcc
     5881 gaagaggccc gcgaaacgga agagaaaatc ctggcggagc ggttcaccct tgaagatttt
     5941 ttgagccagc tccagcagat gaaaaagatg ggcccggtgg agtcgctttt gggcctctta
     6001 cccggcgtga accgcaaggc cttgcagggc gcagcggtcg atgaaaaagc gctcgggaaa
     6061 gtggaggcga tgatttattc catgacccgc gcggaacgca gccaccccga aatcatcgat
     6121 gggtcgcggc ggcgccgggt agccgccgga tcgggcacga tgccctccga cgtgaacggg
     6181 ctcttgaagc agtttttggc catgcagaaa atggtccgtc agttcaagca taccgccgga
     6241 cgccggggga gggggtccat tctctcgggt ctcggtatgc ggtagcgaaa aagagaaaac
     6301 aggaggtggg tttggcggtt aaactgcggt tgcgacgtat gggtgccaag aagcggccct
     6361 tcttccggct ggtggcggcg gagagcacag acccccggaa tggccgtttc ctcgagacgg
     6421 tcgggtacta tcacccgatt gcgaaaccgg ccagggtggt cgtggatgaa gaaaaggttt
     6481 tccagtggct caggcagggc gcccgcccca cggaagcggt gaacgccctc ttccgccgga
     6541 ttggcgtgaa ccggaagtgg caggcgctgg cgaaaggagc cgatgtcgcc acgatgacgc
     6601 tcttgactga gttacccgaa cgcaagaagg ccaagaagcg caaggtcaag caggagcaag
     6661 cccccaccgc ccccgctgcg tagcgcgcgc agctttccta cagcgacggc tgagcagcgg
     6721 aggagggctg tcccacctcg gtgaggtatc cgggaggtgc ggaatgaaag agttcgttga
     6781 aaagatcgtc cgctcgctgg tggagcatcc ggaccaggtg caggtgaccg aggtggaagc
     6841 ggaaaagacg gtagtgctgg aattgcgcgt tgctccggat gacatcggca aggtcatcgg
     6901 caagcacggc cagaatgcca aagcgctgcg cacccttttg accgccgtct cggcccgacg
     6961 gggcaagcga gcggtactgg aaatactcga atagtttcgg cggcgaaacc gagcacgatg
     7021 cgcattgaca tcctgaccat atttccggaa atttttggcg ggtacctcgg gtcgagcctg
     7081 attgccaagg cccaggcaaa gggcttggtg acgatccggc cggtggatct gcggcagttc
     7141 gccaccggca aacatcgcga agtcgatgac gcgccgtacg gcggcggggc ggggatggtc
     7201 tttatggttg agccggttta ccgcgccgtt caggatctcg tcgctgtggc cgggccgcac
     7261 gtttcgcgcc gggtggtgct cttgtcgccg cagggcgtgc agttcgccca gaccacggct
     7321 caagagtttg ccaaaacgga gcatctgatt ctcatttgcg gacggtacaa aggtgtggac
     7381 gaacgggtga aagcgctggt ggtgaccgac gaactttcga tcggcgacta cgtcctcaac
     7441 ggcggcgagg cggcagccat ggtcgtggtg gatgccgtct tccggctctt gccgggcgct
     7501 ctggggaaga gtgattcggc cgagactgat tcggttgcca gcggcctcct ggaatacccg
     7561 gtctacaccc ggccggagga gttcctgggg cagcgcgtgc cggagatttt gctctccggc
     7621 caccacgagg aagtgcggaa gtggcggcgg acccaggcgc tgcgccggac caaagaacgg
     7681 cggccggatc tgttcggccg tttcacctta accgacgaag atcgagaact cctgcaggaa
     7741 aaagatcggg ataaggaaat ataaagggcc gggagcatct ccccgcgaga gaaaaaagag
     7801 aggatagcga catggattta ctcaaagcgg ccgaagcgaa gtttctcaaa gagcggaaag
     7861 tcgcgtttgg accgggcgac accatcaagg tgcacgtaaa gatcaaagag ggcgacaagg
     7921 aacggacgca gatgttccag gggacctgca ttcagaagcg gggaaccggc accggagcct
     7981 cctttaccgt gcggaagatg accgaagggg tgggggtgga gcgggtattc cctttgcact
     8041 cgccttttgt caccaaaatc gatctggtca agcgcgggaa cgtccatcgg gcgaagcttt
     8101 tctatctgcg ccagctgcgg ggaaagtcgg cccggatcga agagaaaaag aaagaagaag
     8161 aaccaacgcc tctccgggcg agagccgaag ccgcagtcgc ctgatctaac ccgccgtgcg
     8221 ccggacgagg acccgaattt ccgagcttag ccggtccgtg ccacagggtg aagctacggg
     8281 cgagattccg tgctctggcg gggttgatct tttcgctttt gagcgggtac tctgggagag
     8341 cggctgggag ctggtggctg gggtggatga agccggccgg gggcctctgg ccggtccggt
     8401 ggtggcctgc gcagtggtat ttccaaagga ttgtccttcc atccccgtga acgattccaa
     8461 aaaactttcc gccgccgagc gtctggcgct catcgggccg attggccgga cagccctttc
     8521 cgtcggcgtg gcggtggtgc agcaggaggc catcgaccgc ttcaatatcc tccaggccac
     8581 cttcagtgcc atgcggcagg cgctgacccg tttgcgctct aagcctgact tagtcatcgt
     8641 ggatggccgg cagcgcatcc ccggcctggc gttcccgcag ttccccttag tgaaaggcga
     8701 cgcccgttcg gcgtcggtcg ccgccgcttc catcgtggcc aaagtcgtcc gcgaccggat
     8761 tatggaggaa atggacgaaa cgtatcccgc ctatggtttt gcccggcaca aaggctatgc
     8821 caccccgtcg caccgcgcgg cgcttttgag attcggaccg tcgccggtgc accgggccag
     8881 ttttttgaaa aaactgttcg cgccggtgca ggagcggctg gcactgtaag tcggaaggcg
     8941 ttcggtcgtt tttttaaccg tatccaggat agtgaagaag tcaaaaaccc aattcggtgc
     9001 cgaagttgaa cagaccgtcg cgcagtacct caccaattgc gggtggcaga ttctggagcg
     9061 taactggcgc tcgggccacc gggaaattga tttaatcgcc cgggacgggc gtgctttagt
     9121 ctttgtggaa gtgaaaggag cgaggtcgct ttcgttcggc cagccggaag agcgggtcga
     9181 tttcgccaaa cgccgccact taaatgacgc ggctttggcc tatcttcagg cgcacgagcc
     9241 ggaggcctcc gaataccgct gggacgtagt gacggtgacc tttgccaaag gcgcgcccac
     9301 catcaaccac ctgcgcaacg cctttacggc ggattgaccg gcggtgaggc agttttcggg
     9361 tgtcctgcgg cggtctcttt ttcctgaagt tttcgggcaa cctcgttcca gatgtgcgcc
     9421 cacttttccg gagagcggtc gactttttga cggaattttt caatttgttc gcgggtgacg
     9481 tgggagcgcg ccaggatatt ttcaatttct ttctgccaga gtccgggatt ggtgctgtag
     9541 cggctttggg cgacggccag gtcagccgtc agggcgatga attcgttttc actcagccgg
     9601 gtctggcgcg gggtgtcgcg gagctggagg atggccacga acagtatggc gaaaaagagg
     9661 acaagtagta tcttgtgggt agacggcgac acaaagcgaa agtaggattg tcgcggtgga
     9721 agtcaagaag gcgtcagcgc aaggcagatt gtttcgggca ttgttgtgaa gcccctgttg
     9781 acaccttact atgaaggaaa gaatggatag tcccaagaag accaacggca atctgccgcg
     9841 cagccgtacc cggcaggtga cggtgggaaa aatcaaactg ggcgggggcg cgccggtggt
     9901 ggtgcagtcg atgtgcacca ccgatacgcg ggatgtccag gccaccttga gccagatccg
     9961 ccagttggcc gaagccgact gcgaattagt tcgcctggcg gttttggacg aaaaagccgc
    10021 cttggcctta aaggagctcg tcccgcacgc gccggtgccg atcatcgccg acatccactt
    10081 caactataag ttggccctgg cggccgtgga ggccggagtg cagaaactga ggctcaatcc
    10141 cggcaacgtc ggcgaagact ggaaggtgcg ggaggtggtc aagcgggtga aggaacgggg
    10201 tattcccatc cgcatcggtg ctaactccgg atctttgccc aaagacatta tcgaaaaata
    10261 cggtaagccg acgccggagg ggattgtgga agccgctttg cgccatgttg ctatcttgga
    10321 ggaaatggat ttccatgaca tcgtcgtttc gctcaagtcg tcggaagtgc gcataacggt
    10381 cgatgcctac cgcctgatgg ccgagactgc gccgcagtat cccctgcaca taggcatcac
    10441 cgaagctggc accgcctggg ccgggacgat taagtcttcc gtcggtctgg gcattctttt
    10501 ggccgacggc attggcgaca ccatgcggat ttcgctcgcc gctgatccgg tggaagaggt
    10561 caaagtcgcc tacgaaatct taaaagacct ggaaatccgc gagcacggcg tcacgctcat
    10621 cgcctgcccc acctgcggcc ggtgcgaaat cgactttatg ccgttagttc agaaagtcga
    10681 agtcgaactg cgcaagatca gagagcccat acgggtctcg gtgatggggt gcgtggtgaa
    10741 cgggcccggc gaatcgcgcg aagccgacgt gggggtctcc gctggccggg gcctggggct
    10801 catcatgcgc aaggacaaag tggtgcgcaa ggtgaaagaa gaggaaatct tcaacgccct
    10861 gatggaggaa gtgcagaaag tgctggcgga gcggcggacg caggcggttg ccacatagag
    10921 gtcgtctgag gatacgttgg tgaagcaaaa gtcaaatgga cagcaagtgg agttcatcgg
    10981 cacaaaactt ttggaagcgg aactggtgcg caacggtttt gaagtggcat acccgattcg
    11041 cgaccgagga attgatttca taatttatca ggacaatcat gggaaacctt tttcggcggt
    11101 tcccattcag gtaaaggcgg cctcgggcga acggtttatt ctcgaccgga aatatgaacg
    11161 catgaagggc ttgatcttgg catacgtctg gggcatcgac aaaaaaccac gttactttct
    11221 gctgacttat cgtgaggctc tctcacttct cggtcgtgcc agaaaaacca attcatggca
    11281 gaaaaaggga atttatgtat acccccacgt tccccaccgg ctgaaggaga aattacatca
    11341 atattatgaa gaccgatgga catggttgcg gaagttactc cgcacaaaaa cacaaaaatg
    11401 aaaggagcat gcaatgccca atccggtagt ccactgggaa atcgccgtca agaacttcga
    11461 caaagccaag gccttctact ccgggctctt cggctggaaa gtcgacagca acaacccgat
    11521 gaactacggc atagtcgata ccgccgcggg cggtatcaac ggcgggattt tccaggccca
    11581 gaagaacatg ccccagaatt acgtcacctt ctacatccag gtggatgacc tgcagaaa
//
LOCUS       DBNGZD010000364         2904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_614170, whole genome shotgun sequence.
ACCESSION   DBNGZD010000364 DBNGZD010000000
VERSION     DBNGZD010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_614170"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="AC16GM_09630"
     CDS             <1..1484
                     /locus_tag="AC16GM_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923966.1"
                     /translation="FFLVFSSASGQGSLLYQKDGTAAGIQFGQSVAVAGDIDGDGKAD
                     FIVGASLASPGGLSHAGSAFIYSGATGALLYQKNGLNSNDFLGWWVAGAGDVNGDGAA
                     DFILGAPFADSGGAIDAGSAFVYSGATGALLYQKNGAVSSDILGSSVAGAGDLNGDGK
                     ADFMVGASLASPAGIPYAGSVFVYSGATGTLLYQKNGPDSNSFLGSSVAGIGDANADG
                     RGDFIIGAPGAGTGKGSVFVYSGSNGALLYQKNGVVGSDGISLGDQLGSAVAGAGDVN
                     GDGRADFMGGAPGADTGKGSVFVYSGSDGVLLYQKNGDVASDGINLGDQLGFAVTRTE
                     DVNGDGRADFMSGSPGADGGGLTDAGSAYVYSGATGALLFQTNGGAAGDLLGYSAAGG
                     GDLDGNGRPDLVIGVPNADPGGLTDAGSALVYCSGPLRGDVNNDAVTDILDLVALVQD
                     VVFEIPLPNPLAGDANCDGVRDIQDLVLLVLYVVFGSPTPCCL"
     gene            complement(1720..2154)
                     /locus_tag="AC16GM_09635"
     CDS             complement(1720..2154)
                     /locus_tag="AC16GM_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR2923967.1"
                     /translation="MLFTKAGEYGLFGLMYLAQKPNGRVTRLADISKAQKVPEKFLAK
                     IFQVLVRKGFVHSHRGTKGGFSLARPANSMTLGEILTAVQGPPAVAECFRPGHECGLY
                     SSCNIRVALEGVQEQLNRSLARFTLADLVPRGSAVAVGARKG"
     gene            complement(2272..>2904)
                     /locus_tag="AC16GM_09640"
     CDS             complement(2272..>2904)
                     /locus_tag="AC16GM_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923968.1"
                     /translation="NTSLLNTPWYLRYARDFLKVDFRMTDGEMNSLSPRVEKGEAAYV
                     SDQVLARLWDLVAKGKIERPLYVAITCSERNRKVLAERLALEGLAYRCVPHPPLSGQN
                     WRVLSTNVVQVYLVTPPANFDWPQCLSPVNRQSYPRVLANYIDIYLAAAQGWLERHDD
                     ARTRDYFWKAFRYAGRVDPARLGSVVQKWRRALPDDPDAREFEEKFPTEK"
BASE COUNT          518 a          874 c          884 g          628 t
ORIGIN      
        1 ccttcttcct cgttttttcc tccgcttccg gccagggaag tctcctttac caaaaagatg
       61 gtaccgctgc gggtattcag ttcggccagt cggtagccgt ggccggggat attgatggcg
      121 acgggaaggc cgattttata gtcggcgcct ctctggccag ccctggcggg ctttcccatg
      181 ccggctcggc ttttatctac tccggggcta ccggcgctct tctgtaccag aaaaacggcc
      241 tcaacagcaa tgactttctg ggttggtggg tggctggggc cggggatgtg aatggcgatg
      301 gtgcagccga tttcattctt ggcgctccct ttgccgactc gggcggggcg atcgatgccg
      361 gttcggcttt tgtctactcc ggggctaccg gcgctctcct ttatcagaaa aacggcgcgg
      421 tttcctccga catattgggg agttcggtgg ccggagccgg agacctgaat ggcgacggca
      481 aagccgattt tatggtcggc gcctctctgg ctagcccggc cgggattccg tatgccggct
      541 cggtttttgt ctattccgga gccaccggta ctctcctgta ccagaaaaat ggccccgata
      601 gcaatagctt cctgggcagt tcagtagccg gaatcggaga tgcaaatgcg gacgggagag
      661 gcgactttat catcggcgct ccgggagccg gcaccggcaa gggttcggtc tttgtctatt
      721 ccgggagtaa cggtgcgctg ctctaccaga aaaacggcgt cgtcggcagt gacgggatca
      781 gcctaggcga ccagttgggc tctgcggtgg ccggggccgg cgatgtgaat ggtgatggga
      841 gagccgactt tatgggtggc gctccgggag ccgacaccgg gaaaggttcg gtctttgtct
      901 attccgggag cgacggcgtt ctactctacc agaaaaacgg cgacgtcgcc agcgacggga
      961 tcaacctggg tgaccagttg ggctttgcgg tgacccgtac ggaggatgtg aatggtgacg
     1021 ggagagccga ctttatgagc ggctctccgg gtgccgatgg cggcggcctc actgacgctg
     1081 gttctgcgta cgtctactcc ggcgccacag gcgccctgct tttccaaacg aacggcggcg
     1141 ccgccggtga ccttttgggc tattcggcgg ccgggggcgg ggatctggat gggaatggta
     1201 ggcccgatct cgttatcggc gtacccaacg ccgacccggg cggactgaca gatgccggct
     1261 cagccctggt gtactgctcc ggaccgctgc ggggcgatgt gaacaacgat gcggtgaccg
     1321 acattctgga ccttgtggcg ttggttcagg atgtcgtgtt cgaaatccct ctgcccaacc
     1381 ccctggcagg cgatgcgaac tgcgacggcg tcagggatat tcaggacctt gttctcttag
     1441 tcctgtacgt cgtgttcggc agtcctactc cctgctgtct ctaactaggg tatcgtgagt
     1501 ccgcttgagg aggaccgaac cacagtccaa ctccttctac aagtgaccgg cgggcaagaa
     1561 aactatagag gtaaacccac ctgagagaca ggttcacccc ttcttctatt caaattgata
     1621 acaaccaata cccataggtt aggcatcttg cctgaccggt ctccgaggca gaaagcctcg
     1681 gctatggtaa cctaatttct ttcgcctgtc tcgaaccggc taccctttcc gggcgccaac
     1741 cgccaccgcc gagccccggg gaacgaggtc cgccagggtg aaccgcgcca gcgaccggtt
     1801 caactgctcc tgaacccctt ccaaagccac ccgaatgttg caactgctgt agagtccgca
     1861 ctcgtggccg gggcggaagc actcggccac cgccgggggc ccctgcaccg cggtcaggat
     1921 ttctcctaag gtcatgctgt tggccgggcg cgccagggaa aaaccgccct tggtcccccg
     1981 gtgggagtgg acgaatccct tccgcaccaa aacctggaaa atcttggcca aaaacttctc
     2041 cggcaccttc tgggctttgg aaatgtcggc cagccgggtc accctgccgt tgggcttctg
     2101 cgccaggtac atcaggccga acagtccgta ctcacctgct ttggtaaaga gcatctgcca
     2161 cctcgcttcc tttcccgtca accgtgaccg cttggggcct gaatcggata tcgatattca
     2221 ggtcttgtag tcgtaatata gtatacgaga attgtccgct cctgttttac cttatttttc
     2281 ggttgggaat ttctcttcga actcgcgcgc gtccgggtcg tccggcaggg cccggcgcca
     2341 cttttgcacc acactcccca gccgggccgg gtcgaccctg cccgcatagc gaaaggcttt
     2401 ccagaaatag tcgcgggtcc gcgcatcgtc gtgcctttca agccatccct gggcggcagc
     2461 caggtagata tcgatgtagt tcgccagtac ccgtgggtag ctctgccggt tgaccggcga
     2521 gagacactgc ggccagtcga aattggccgg gggtgtgacc aggtacacct gcactacgtt
     2581 ggtgctcaag acgcgccagt tctggccact tagcggcggg tggggcacac agcggtaggc
     2641 cagcccctcc agcgccagcc gctccgccag aactttgcgg ttccgctcgc tgcaggtgat
     2701 ggccacgtag agcggccgct cgattttccc cttggccacc agatcccaca ggcgggcgag
     2761 cacctggtcg gagacgtacg ccgcttcccc cttctccacc ctcggggaga gactattcat
     2821 ctccccgtcg gtcatccgga aatcgacttt tagaaaatcg cgcgcgtacc gcaggtacca
     2881 gggagtattg agcaggctcg tatt
//
LOCUS       DBNGZD010000365         2866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1586242, whole genome shotgun sequence.
ACCESSION   DBNGZD010000365 DBNGZD010000000
VERSION     DBNGZD010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1586242"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="AC16GM_09645"
     CDS             complement(<1..1289)
                     /locus_tag="AC16GM_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923969.1"
                     /translation="MTAILLTKYFIRYLVLFFLIGFLMTLEAIGRLSGTINRDSTIGS
                     GADTVTGDLTIAPGAIVTLTAPLILWISAHSDDQHSGLDPNRVEVNVQGVLKARGTSS
                     ARIIWKSTNAVSPSRADWVGVRVWKQAKTELFYNDFRNAESAVTDSSRVGNDSIWGCI
                     FEGNTIGLTLEHPSQDTTDTIAFVGGCLFQNCKCGVAAFLGSFNRTLAYVDSSTFFDV
                     DSGVVGYAGASGRISRNRIENDTAAVGINLFDWNDNDTIYIVSNTIRGFYTGAHLRFN
                     NLLAVVADNIIVTGEGTPRSSTAIDGFNSKPTLDALPLIKFRRNKILGFASFGFSIQH
                     GDPGDPAPSIDLGDMSLGDPGLNTIVTPVAGAKAINNFGPISLKAQGNWFGTANPPSS
                     LFSGSVDASQPLTAPRDSASGKVGVYGLTSITWGPGKA"
     gene            complement(1295..2044)
                     /locus_tag="AC16GM_09650"
     CDS             complement(1295..2044)
                     /locus_tag="AC16GM_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2923970.1"
                     /translation="MRKFLAAVLVAVWVGLGAGAPWAGQEPFAPTLAVDPGIPDTVRF
                     TLQCQQDNGIFTVDIFVFNDENLSGVNLKVLTDSVTFFPKLDSITFYATRLENPLVLD
                     ARSANVAVSNGIPPDSLVILLTVFGEGPLPPGMGPICRLWFSRGLEGKSFLFITQPPG
                     AIIPSIRFTEPSINTFRPVSFNQEVRLLLAYGDLTGDSLVNVADAVYLLSAVVFLSED
                     FRGGDLNCDGVVDIVDIVMLIYCVVLQLPCL"
     gene            complement(2151..>2866)
                     /locus_tag="AC16GM_09655"
     CDS             complement(2151..>2866)
                     /locus_tag="AC16GM_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR2923971.1"
                     /translation="TSAFRAYALADHYRAKGAYVALGGLHVTALPDEAARHADSIFLG
                     PFLPVPIVSKKEKGGKEVIFFDSKRPQSIGKVHGFYGNFGVFVRAYTYIRMHGPDGLR
                     QNSENAVINANYLLARLKDAYDLPYPRFCMHEFVLSGSRQKEKGVRTMDIAKRLLDYG
                     VHAPTVYFPLIVPEALMIEPTETESRESVDQFAEAMLAIAREVEENPDLVRTAPHNTP
                     VARLDEAKAARELNLRWPG"
BASE COUNT          736 a          757 c          706 g          667 t
ORIGIN      
        1 gccttacccg ggccccaggt aattgaggtg aggccataca ccccgacttt tccacttgcc
       61 gaatcgcgcg gggcagttaa cggttggctt gcgtctacag aaccagaaaa aagtgaactg
      121 ggcggattcg cagtgccaaa ccagttgcct tgggccttga gggaaatggg cccaaaattg
      181 ttaatggctt ttgcacctgc tactggagta acaatagtat tcaatcccgg atcgccaagg
      241 gacatatccc ccaagtcgat tgaaggtgct gggtcaccgg gatctccgtg ttgaatcgag
      301 aagccgaagc tcgcgaaccc aagtattttg ttccggcgaa actttattaa aggcaacgcg
      361 tccaatgtag gtttagaatt gaaaccatct atagcagtac ttgaacgcgg agtcccttcc
      421 ccggtaacaa ttatgttgtc cgcaacgacc gcaagtaaat tgttgaatct aagatgtgcg
      481 ccggtataaa aaccccttat cgtgtttgag actatgtaaa ttgtgtcatt atcattccaa
      541 tcgaataggt taattccaac agcggcagtg tcattctcta tcctgtttcg ggaaatacga
      601 ccggatgcac cagcataacc gactacacca gagtcaacgt caaaaaaagt gctactatcc
      661 acgtaagcca acgtcctatt aaaggaacca agaaacgcag ccactccaca tttacaattc
      721 tgaaaaaggc aaccgccgac aaacgcgatc gtatcagttg tatcttgaga aggatgttct
      781 aacgtgagtc cgatggtatt gccttcaaag atacatcccc atattgagtc gttgccaacc
      841 cgactgctat cggtgaccgc cgactctgcg ttcctgaaat cgttataaaa gagctctgtt
      901 tttgcttgct tccaaactct tacaccaacc caatcagccc ttgatggact aactgcattg
      961 gttgatttcc aaataattct ggccgaactc gttcctcttg ctttcagtac gccctgtaca
     1021 tttacttcca cgcggtttgg gtctagcccg gagtgctggt catcgctatg cgctgaaatc
     1081 caaagaatca ggggtgcagt aagagtcaca atcgcccctg gggcaatagt caaatcacca
     1141 gtaaccgtgt ctgctcccga accaatagtg gaatctcgat tgatggtacc cgataagcgg
     1201 cctatcgctt ctagtgtcat taagaaccct atcaagaaga atagaacgag gtatcgaata
     1261 aagtacttgg ttagtaagat cgccgtcatt gacgtcatag gcagggtagt tgaagaacca
     1321 cgcagtaaat caacattaca atgtccacaa tatccactac cccatcacag ttgagatccc
     1381 ctccccgaaa gtcctcactc aaaaatacca cagcgcttaa taagtagaca gcatcggcta
     1441 cgttcaccag agaatctccc gtaagatcac cataggccaa aagtagtcgg acctcctgat
     1501 taaatgacac cggccggaaa gtgttgatcg agggttcagt gaaacgaatg ctgggaatga
     1561 tggctccagg aggctgggtg atgaatagaa aacttttgcc ctccagaccg cgggaaaacc
     1621 aaagtcggca gattggcccc attcctggtg gcaatggtcc ttctccgaaa accgttagta
     1681 aaatgactaa tgaatcgggt ggtataccat ttgataccgc gacattagca ctccgtgcgt
     1741 ctaagactaa aggattctcg agacgagtgg cgtaaaacgt aatcgaatca agcttgggga
     1801 aaaaagtgac tgaatcggta aggactttca gattgacgcc cgaaagattc tcatcattga
     1861 atacgaagat gtccaccgtg aagatgccat tatcctgctg gcactgcagg gtaaaccgca
     1921 cggtgtcggg aatgccgggg tctacggcta atgtcggagc aaacggttct tgccccgccc
     1981 agggcgcacc cgcccccagc cccacccaaa ccgccacgag aactgcggcc aagaacttcc
     2041 tcatcttctt tccttatgcc ccactctctc tgagtggccg ctgtctaaat ataacctacc
     2101 gaggagtaga agtaaagtct atttggagaa gaagattggt ttaatctcgc ttatccgggc
     2161 cacctcaggt tcaattccct tgccgccttg gcttcatcta agcgcgccac gggcgtattg
     2221 tgcggtgcgg tgcggactaa gtcggggttt tcttccacct cgcgggcaat ggcgagcatc
     2281 gcctcggcga actggtcaac gctttcccgg ctttcggttt cggtcggctc aatcatcagc
     2341 gcttcgggca caatcagggg gaagtagacc gtcggcgcat ggacaccgta atcaaggagc
     2401 cgcttggcaa tgtccattgt cctcacccct ttttccttct gccggctgcc ggagaggaca
     2461 aactcgtgca tgcaaaagcg cgggtagggg aggtcatagg catctttcaa ccgcgcgagg
     2521 aggtagttgg cattgatgac ggcgttctcg ctgttttggc ggagaccgtc cggcccgtgc
     2581 atccggatat aggtgtaggc ccgcacaaag acgccgaagt taccgtaaaa gccgtggact
     2641 ttgccgatcg actgcgggcg cttgctatcg aagaagataa cttcctttcc tcctttttct
     2701 tttttactca caatcggaac gggcaagaac ggccccagga agatactgtc ggcgtgccgc
     2761 gccgcttcgt ccggcagcgc cgtgacgtgc agcccgccaa gcgctacata agcgcctttt
     2821 gcgcgataat gatcggccag cgcataggcc cggaatgccg acgtga
//
LOCUS       DBNGZD010000366         1517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1238145, whole genome shotgun sequence.
ACCESSION   DBNGZD010000366 DBNGZD010000000
VERSION     DBNGZD010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1238145"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="AC16GM_09660"
     CDS             complement(<1..314)
                     /locus_tag="AC16GM_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923972.1"
                     /translation="MRRYWKIGAGIVALIIVIIIAASIFSGKVDLPTAAAQSGEFVIT
                     LTENGELKAQRAVSVVAPRVRGKMLITKLAPEGSVVKAGDFLIQFDASEQHATLQDNQ
                     ANL"
     gene            complement(411..1349)
                     /locus_tag="AC16GM_09665"
     CDS             complement(411..1349)
                     /locus_tag="AC16GM_09665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR2923973.1"
                     /translation="MSNPGGPNRRTSLIQKIFRDLIFYKIVVTAWWLRRLPYLWALAL
                     GRLWGLLVFDLFRIRRAVTLNNLRRALGREKPKAEILSIARRTYQNLGQAFVEFCLIP
                     RLVRKRRLSGLVTSETFQFVQQAASGGKGCLLMSGHFGNWELSGGFICQSGLPVDFVV
                     KEIRNPKLDRWVSRYRQLLGVGRIPMDKAPREVITATRTGRCVAMLADQYAGAEGVIV
                     DFFGLPTSTPKGPAALALKLDIPIVCGFLIREGGGRFRLIVEPPIRPSPTGDRERDIQ
                     RLTQEFTRILEKYVRLYPDHWLWTHRRWKDLDRKEN"
     gene            complement(1291..>1517)
                     /locus_tag="AC16GM_09670"
     CDS             complement(1291..>1517)
                     /locus_tag="AC16GM_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923974.1"
                     /translation="EAEYLAYLKKHYNWVAILSDFTVLWIFLAFLLILAFILYWRRKR
                     KILKAWQKKESFEQPRWSEPTDEFNPEDLP"
BASE COUNT          338 a          416 c          433 g          330 t
ORIGIN      
        1 aggttggcct ggttgtcctg cagggtggcg tgttgttcgg aggcgtcgaa ttgaatcaag
       61 aaatcgcccg ctttgaccac gctcccctcc ggcgcgagtt tggtgatgag catcttcccc
      121 cggacacgcg gggccaccac ggagacggcg cgctgggctt tcagttcgcc gttttcggtc
      181 aaggtgatga cgaattcgcc cgactgcgcc gccgccgtgg gcaggtcaac cttgccggaa
      241 aaaattgacg cggcaataat gatgacaatg ataagggcga ctatgcccgc accgattttc
      301 cagtagcgtc gcatgatgtc tggtagagtt atacccatta acaattcctt tagactaaac
      361 ttttcccgac gctcgggaga gcgtcactac actctttctc tctccagttc tcaattctct
      421 tttctatcga ggtccttcca cctccggtgt gtccaaagcc agtggtcggg gtataagcgg
      481 acgtatttct ccagaattcg ggtgaactct tgcgtcaggc gctggatatc gcgctctcgg
      541 tcaccagttg gggagggccg aatcggtggc tcgacaatca ggcggaagcg accacctcct
      601 tcacggataa ggaaaccaca gacgatggga atgtcaagtt tgagcgccaa cgccgccggg
      661 cctttgggag tcgaggttgg cagaccaaag aagtcgacaa tcaccccttc tgcgcccgcg
      721 tactgatccg ccaacatcgc cacacagcgg ccggttcggg tagccgtaat gacttcccgc
      781 ggtgctttgt ccatggggat gcggccgact cccaagagct gacggtagcg gctgacccag
      841 cggtcgagtt tcggattgcg gatttccttg accacgaaat cgaccggaag acccgactgg
      901 cagatgaaac cgcccgaaag ttcccagttg ccgaaatggc cggacatcag aaggcagcct
      961 ttcccgcccg atgcggcctg ctggacgaat tgaaaagttt cagaggtgac caggccggaa
     1021 agtcgtctct ttctgaccag tctcggtatc aggcaaaact caacgaaggc ctggccgagg
     1081 ttctggtagg tgcggcgggc gatggagagt atttcggctt tgggtttttc ccgaccgaga
     1141 gcgcggcgga ggttgtttaa ggtcactgcg cgccggatgc ggaagagatc aaaaaccaga
     1201 agtccccaca aacggcccag cgccaatgcc cacaggtaag gcaggcgtcg cagccaccag
     1261 gcggtaacga ctattttgta aaaaattaga tcacggaaga tcttctggat taaactcgtc
     1321 cgtcggttcg gaccaccggg gttgctcaaa ggattccttt ttctgccacg ctttcagaat
     1381 tttccttttg cgccgccagt agagaataaa agccaggatg agcaggaacg ccagaaaaat
     1441 ccagagtacc gtgaaatcgc tcaaaatcgc cacccagttg tagtgttttt tcaggtaggc
     1501 gagatactcg gcttcgt
//
LOCUS       DBNGZD010000367         3482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_614238, whole genome shotgun sequence.
ACCESSION   DBNGZD010000367 DBNGZD010000000
VERSION     DBNGZD010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_614238"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16GM_09675"
     CDS             complement(<1..226)
                     /locus_tag="AC16GM_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PmbA/TldA family metallopeptidase"
                     /protein_id="HIR2923975.1"
                     /translation="MLTRERCQEICEKVFKFSNADETEVSFGGGESALSRFANNEIHQ
                     NVSEEGYYLSVRVVYGPKTGRASTNRYDDES"
     gene            complement(323..1777)
                     /locus_tag="AC16GM_09680"
     CDS             complement(323..1777)
                     /locus_tag="AC16GM_09680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2923976.1"
                     /translation="MRDVLKLALDAASGSGVSYAEARIVQEKSQSITTKNGKVGSIRQ
                     DETFGLGIRVIAHGAWGFAATDDLSPKNVKRIAKKAVAIAKASALCKTHDIQLASEEK
                     HVSVWRAPFTKDPWAVPLSDKVDLLLKVDETCRKVKGVTAVEGNMNFVRETKYFLSSL
                     GSDIEQHRFRTGAGFSVSSFTDGELFERSYPASFGGQYSCKGYEMVDEINLLDNAQRI
                     AEEAVALHSAPQCESGVKDLILETSQLALQIHESIGHPIELDRVLGTEANYAGTSFLT
                     LDKLNKLQYGSKHMNVVADATLAHGQALGTFGFDDEGVPAQRNWIVKDGLFVGYMTSR
                     ETAPVVGASRSNGCMRADGWNRIPLIRMTNVSLEPGDGKFADLVADTKDGIYMSTVRS
                     WSIDDKRYNFQFGPEIAWEIKDGKLGRMLKNPSYGGITTEFWNSMDAVCGKPEWVLWS
                     VPNCGKGQPGQSMGTGHGCAPARFRNVKIGVAFG"
     gene            complement(1937..2995)
                     /locus_tag="AC16GM_09685"
     CDS             complement(1937..2995)
                     /locus_tag="AC16GM_09685"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR2923977.1"
                     /translation="MYNIAVVPGDGIGPEVINEALKVLKAVSELDKFDYRLQQYPYGS
                     EHYLSTGELVPGTWREELQKFDAILLGAIGDPRVEPGLVERAVIMTIRFGLDLYVNLR
                     PIKLYAEHLCPIKGKKPEDVDFIVVRENTEDLYAGIGGHLKKDTPDEVAIQEMIFTRK
                     GCERVVRYAFELARERNRQKKLTLVDKANAIRAMDIWTRTFEEVGQEYPDIIRDHAYV
                     DAACMWMIKNPEWFDVVVTNNIFGDIITDLGAMVQGGMGVAASGNIHPGKVSMFEPIH
                     GSAPKYKGKNVANPIAAIMAGAMLLDYVGEKKSAARVEKAVEHLLVTQRIKSLDAGSG
                     LSTTQIGDMVVAEIQKQK"
     gene            complement(3231..>3482)
                     /locus_tag="AC16GM_09690"
     CDS             complement(3231..>3482)
                     /locus_tag="AC16GM_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923978.1"
                     /translation="GLNMGIGGAARDFGWWAGWSAGFQDEETGLLLYDGNGFLGRADI
                     RIAPGWLVNVTGRAGASSGTFEGGVSLGLTKRWALPVQK"
BASE COUNT          695 a         1030 c          989 g          768 t
ORIGIN      
        1 tcgactcgtc gtcgtagcgg ttggtagagg cgcggccggt tttcggcccg taaactaccc
       61 gtaccgagag gtagtatcct tcttcggaaa cgttctggtg aatttcgtta ttggcaaagc
      121 gggataaggc gctttccccg ccgccaaaag agacctcggt ctcgtcggcg tttgagaatt
      181 tgaaaacctt ctcacagatt tcctggcaac gttcgcgggt tagcattctt cgccttatgt
      241 cggtagtccg gctcgtctct gagccggacg gacgggctcg gggacgagcc cgtctaccaa
      301 aacggaccgg tatccagatt tactatccaa acgccacgcc tatcttcaca ttcctgaacc
      361 gcgccggcgc acagccgtgg ccggtgccca tcgactggcc gggctggcct ttgccgcagt
      421 tgggcacgct ccagagcacc cattcgggtt tgccgcagac ggcgtccatt gagttccaga
      481 attcggtggt gatgccgccg taggaaggat tcttgagcat ccgccctaat ttgccgtctt
      541 tgatctccca ggcaatctcc ggcccgaact ggaagttgta gcgcttgtcg tctatcgacc
      601 aggagcggac cgtggacatg tagataccgt ctttggtgtc ggcgacgagg tcggcgaact
      661 tcccatcgcc cggttctaag gaaacattgg tcatgcgaat caagggaatc cggttccagc
      721 cgtcggcgcg catgcagccg ttggagcggg aggcgcccac caccggcgcg gtctcccggg
      781 aggtcatata gccaacgaaa aggccgtcct tgacgatcca gttgcgctgg gcgggcaccc
      841 cctcgtcgtc aaaaccaaag gtgcctaacg cttgaccgtg cgccagcgtc gcgtcggcca
      901 ccacgttcat atgcttcgag ccgtactgca acttgttcag tttgtccagg gtcaaaaacg
      961 aggtcccggc gtagttcgcc tcggtgccca ggacgcggtc caactctatc ggatggccga
     1021 tcgactcgtg aatttgcaaa gctaattgcg aagtttccag aatcaagtct ttgacgcccg
     1081 actcgcactg cggcgcggag tgcagggcca ccgcttcttc ggcaatgcgc tgggcgttgt
     1141 cgagcaggtt gatttcatcc accatctcgt accctttgca ggagtactgg ccgccgaaag
     1201 aagccgggta ggagcgctcg aacagttcgc cgtcggtgaa ggaggagacc gaaaagcccg
     1261 cgccggtgcg gaaacggtgc tgctcgatat cggagcccag agaggagaga aagtactttg
     1321 tttcccggac gaagttcata ttcccttcga ctgcggtgac tcccttcacc ttgcggcagg
     1381 tttcatccac tttgagcaaa agatcgactt tgtcggagag cggcacggcc caggggtctt
     1441 tggtgaaggg cgcccgccag acggaaacgt gtttttcctc cgacgccagt tggatgtcgt
     1501 gagttttgca cagggctgaa gctttagcga tggcgaccgc ctttttagcg atccgcttga
     1561 cgtttttggg ggaaaggtcg tcagtagcgg caaaccccca ggcgccgtgg gcgatcaccc
     1621 gaatgcccag gccgaacgtt tcatcctgcc ggatggagcc gaccttgccg ttcttggtgg
     1681 tgattgactg ggatttttcc tggacgatcc gggcttcggc gtacgacacg ccggaacccg
     1741 acgcggcatc cagcgccagt ttcaaaacat cacgcaaaat ttaacctccg catcagccat
     1801 tcactccgga tgaccggcta atatagccag acaaacgctg cgcgccagca gaatttttga
     1861 aagaaaactc cctattgtga aagaaatcac aaaagagaag gttttgattg accggcaaac
     1921 ttcgggcggg gcgtttctat ttttgttttt gaatttcggc aacgaccata tcgccgattt
     1981 gtgtggtcga gagaccggag ccagcgtcga gcgatttgat tctttgcgtg accaaaagat
     2041 gttcgaccgc tttctcaact cgcgcggctg atttcttttc gccgacgtaa tccaaaagca
     2101 tcgccccggc cataatcgcc gcaatcgggt tggcgacgtt tttgcctttg tacttcggcg
     2161 cagagccgtg aatcggctcg aacatcgata ccttgcccgg gtggatgttc ccggaagcgg
     2221 ccacccccat cccgccctga accatagcgc ctaaatcggt gatgatgtcg ccgaagatgt
     2281 tgttcgtcac gaccacgtcg aaccactccg ggtttttgat catccacatg caggcggcat
     2341 cgacataggc gtggtcgcgg atgatgtcgg ggtattcctg cccgacctct tcgaacgtcc
     2401 gggtccagat atccatcgcc cggatggcgt tggctttgtc cacgagcgtc aattttttct
     2461 ggcgattgcg ctcgcgggcc agttcaaaag cgtagcggac tacccgctcg caccctttgc
     2521 gggtgaaaat catttcctgg atcgccacct cgtcgggggt gtctttcttc aagtgcccgc
     2581 cgatcccggc gtacaggtct tcggtatttt cgcgcaccac gatgaaatcg acatcctccg
     2641 gctttttgcc cttaatcggg cagagatgtt cggcgtagag tttgatcggg cgcaggttga
     2701 cgtagaggtc cagcccgaag cggatggtca taatcaccgc ccgctctacc aaccccggct
     2761 ccacccgcgg atcaccgatg gcgcctaaga ggatcgcgtc gaacttttgg agctcctccc
     2821 gccaggtgcc ggggactaac tcgcctgtgg agaggtagtg ctcgctgccg taggggtact
     2881 gctggaggcg gtagtcgaac ttgtctaatt cggagacagc cttgaggact tttagcgctt
     2941 catttatgac ttccggcccg ataccatccc cggggacaac agctatgttg tacatgggaa
     3001 gcgaagatag aaagaattgg ggaggattca aatacgaatt atcaccacgg aggcgcttcc
     3061 tttcccgcgt aggccggact tttagtcctg cagcacagcg cgtagccgag gcgccccgcc
     3121 tcggacaaag gcggcactga aagtgccgcc tacgcggaga gcggtcgctc ctttccgcgt
     3181 atgccggtta aagactcgta ccgaggtttc agcccggccg gagcagattt tcacttctgg
     3241 acgggtaggg cccagcgctt ggttaaaccc aaactgacac cgccctcgaa cgtacccgaa
     3301 cttgccccgg cccgtccggt cacgttcacc agccaccccg gcgctatcct tatatcggct
     3361 cggccgagga agccgttgcc gtcataaagc aaaagcccgg tttcctcgtc ctggaaaccc
     3421 gcgctccaac cggcccacca gccgaaatca cgtgccgctc cgccaatccc catattgaga
     3481 cc
//
LOCUS       DBNGZD010000368         3446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_474599, whole genome shotgun sequence.
ACCESSION   DBNGZD010000368 DBNGZD010000000
VERSION     DBNGZD010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_474599"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /locus_tag="AC16GM_09695"
     CDS             complement(<1..2012)
                     /locus_tag="AC16GM_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR2923979.1"
                     /translation="MVGKSGSTVFPSVKAIRQRFEQAGTVKRVLQAVTGDEKKVKVTG
                     LSGSAVAFLTVALFEHHKKPLCFLTSDPDSAEKAAADLAALLPGEAVHLFPERGVVPY
                     EFRAPHEEVVGRRLQTLFALLRHEHSLVVASLPAVLEQTIPPEKLKAQSLFLRPDDQI
                     DLDEVARRLLALGFSRVPLVEEVGGFAIRGGIVDVFPCTAEDPLRLEFFGDRLDSIRF
                     FSVLDQRSITRTDSTLILPQKEFYLPPAQLEEHLEKLDPRLEAEIREKFAYEPDQPGI
                     EWLAPLFGLERAPLFDYFPENFLLVVDEGETIKSKLGEIFEQAEKLSTELKRRLPLQL
                     SPQSLYGAPEQVWMNIESYAGVEKLPTGQEVEGVIDLETKDQPAFGGKIEFVRAKVRE
                     LGQYQYQTFITCETPGQAERLTELLVPMEEEIFLCTPSLAEGFLFPEAQLAVFTDHQI
                     FRRPQHRWPKRRFREGVALSAYTNLQPGDYVVHIDYGIGRFLGIETLIVEGRKRDCLA
                     ILYQEGDKLYVPTEEFNRVQKFIGHEGEPTLSRLRSDTWEKLKARTKEALLEMAGELI
                     ALSAERKAKPGAAFSPDTPWQKELEAAFIYADTPDQLAAIEAIKSDLEKPAPMDRLVC
                     GDVGFGKTEVAIRAAFKAVQDGKQVAVLVPTTILAQQHYLTFSERLL"
     gene            complement(2151..3047)
                     /gene="speB"
                     /locus_tag="AC16GM_09700"
     CDS             complement(2151..3047)
                     /gene="speB"
                     /locus_tag="AC16GM_09700"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR2923980.1"
                     /translation="MPNFLGIPDRFSNPDRARFLILPAPYEGTVTYKKGTKDGPAAII
                     AASQQVELFDEELGFEPYRAGIATLAALEVTPEGPKQMVERARRTVRELLRKKRMVIF
                     LGGEHSLTTGAVRAYQEKFNDLSVLQLDAHADLRDTYDNSPYNHACTLRRVREICPAV
                     QVGIRNLSAEENKFISQKGIQLYYAKDIWNTNDWIDEAIGKLPRNVYVTIDLDVFDPS
                     CMPAVGTAEPGGLSWYQVLEVLRKTVEEKNVVGFDVMELSPIPEMVAPDFLAAKLVYK
                     MIGYLMAKERILGRQVKPKHRR"
     gene            complement(3218..>3446)
                     /locus_tag="AC16GM_09705"
     CDS             complement(3218..>3446)
                     /locus_tag="AC16GM_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923981.1"
                     /translation="SGPHYIALTPDEKRLVITDYFLNEDDAGKVHAEGDHKIHVAVVS
                     ANDLVLDPKFRLDFNTAIATGPARPHGVAFK"
BASE COUNT          733 a          965 c          983 g          765 t
ORIGIN      
        1 agcagcctct cgctgaaggt gaggtagtgc tgctgagcta aaattgtcgt aggaacaaga
       61 acagccacct gtttgccgtc ctgcactgct ttaaacgcgg cccggatcgc cacttcggtc
      121 ttgccgaagc cgacatcacc gcagaccagc cggtccatcg gtgccggctt ttctagatcg
      181 cttttaattg cttcaatggc ggccagctgg tccggggtat cggcatagat gaacgctgcc
      241 tccaactctt tctgccaggg ggtatcggga gaaaaagcgg ctccgggctt ggctttgcgt
      301 tcagcggaga gggcgatcaa ctctcccgcc atttccagga gcgcttcctt ggtgcgggct
      361 ttgagtttct cccaggtatc agaacgcagg cgtgagaggg tgggttcccc ctcgtgaccg
      421 atgaacttct gcacccggtt gaactcttcg gtgggaacgt agagcttgtc cccttcctgg
      481 tagagaatgg ccaggcaatc gcgcttgcgg ccttccacaa tgagcgtctc aatccccagg
      541 aagcggccga tgccgtagtc gatgtggacc acgtaatcgc ccggctgcaa attggtgtag
      601 gcggagagcg ccactccttc ccggaagcgg cgtttgggcc agcggtgctg cggccggcgg
      661 aaaatctggt gatcggtaaa gaccgccagt tgcgcctcgg ggaaaaggaa gccttcggcc
      721 aaagaggggg tgcagaggaa aatttcctcc tccatcggaa ccagaagttc ggttaaacgc
      781 tcggcctggc cgggagtttc gcaggtaatg aaggtctggt actggtactg acccagttct
      841 ctcactttgg ctctgacaaa ctcaattttc cccccgaaag cgggttggtc cttcgtctcg
      901 aggtcgatga ccccctccac ttcctgaccc gtgggaagtt tctcaacccc ggcgtacgac
      961 tcaatgttca tccaaacctg ttcgggagca ccatagaggg actgcgggga aagctggagc
     1021 ggcaatcgcc ttttgagttc ggtggagagt ttttcggctt gttcgaaaat ttcccccagc
     1081 ttgcttttga tcgtttcccc ttcatcgacc acgagcaaaa agttttcggg gaagtaatcg
     1141 aagagcgggg cccgctccaa tccgaagagc ggggccagcc attcaattcc cggctggtcc
     1201 ggttcgtagg caaatttctc ccgaatttct gcctcaagac gcgggtcaag tttctccagg
     1261 tgctcttcga gttgtgcggg cggcaggtag aactctttct gcggcaaaat cagtgtcgaa
     1321 tcggtgcggg taatcgagcg ttgatctaat accgaaaaga agcggatgga gtcgagccgg
     1381 tcgccgaaaa attccagccg caaaggatct tcggcggtgc aggggaagac gtccacgatg
     1441 ccgccgcgga tggcgaagcc gcctacttct tccaccagcg gcacgcgcga gaagcccagc
     1501 gccaaaagcc gccttgcgac ctcatcaaga tcgatctgat cgtccgggcg caaaaagagg
     1561 ctctgcgcct ttaacttttc cgggggaatg gtctgctcca gcactgcggg cagggaggcc
     1621 acgaccagtg aatgctcgtg acgcaaaagg gcaaagagcg tctggagcct gcggcccacg
     1681 acctcttcgt gaggcgctct gaattcgtaa ggcacaaccc cgcgctcggg aaagaggtga
     1741 accgcttctc ccggcaatag cgcagccaag tcggcggcgg ccttttcggc tgagtccggg
     1801 tcggacgtga ggaaacagag cggtttttta tggtgctcga atagagccac ggtcaaaaaa
     1861 gctacagccg agcccgacag gccggtaact ttgacttttt tctcatctcc ggtaaccgcc
     1921 tgcaggaccc gtttgaccgt gccagcttgc tcaaaccgtt gacgaatggc cttgacggat
     1981 ggaaaaacgg ttgaaccact ctttccaacc atgttttcgc tgaccggtgc tttcacacta
     2041 ttcccacgca tagccgaaac gccaaaaccc cgcctccgcg cggagccggg ggtgttcaca
     2101 atcggtgaca ttccaagtta acggtttacg ggacaaagaa taagccgctc ttacctcctg
     2161 tgtttcggtt tgacttggcg gccgagaatt ctctccttgg ccatcaggta tccaatcatc
     2221 ttatatacga gtttggccgc cagaaagtca ggcgccacca tctccggtat cggcgacagc
     2281 tccataacgt caaaaccaac cacatttttc tcttcgacgg ttttccgcag cacctccagc
     2341 acctggtacc aagacagccc gcccggctcg gctgtaccaa cggcaggcat acatgagggg
     2401 tcaaagacat cgagatcgat agtcacgtag acgttccggg gaagctttcc aatagcttcg
     2461 tcaatccaat cgttggtatt ccaaatgtcc ttggcgtagt agagctggat tcctttttgg
     2521 gaaatgaatt tgttttcttc agcgctgagg ttgcgaatgc ccacctgcac cgccgggcat
     2581 atttcccgca cccggcgcag ggtgcaggca tggttgtaag gggaattgtc ataagtgtcg
     2641 cgcaggtcgg cgtgggcgtc cagttgtaag actgagagat cattgaattt ttcctggtac
     2701 gctcgcaccg cgccggtggt aagggaatgc tccccgccca gaaaaatgac catccgcttc
     2761 tttcgtagca gttcccgaac cgtccgccgg gcccgctcga ccatctgctt cggcccctcg
     2821 ggggtaactt caagcgcagc caaggtagca atgccggcgc ggtacggttc aaatcccaac
     2881 tcctcgtcga aaagttccac ctgctgcgac gctgcgatga tggctgctgg tccgtctttt
     2941 gttcctttct tgtaggtgac cgtgccctcg tacggcgcgg ggaggatgag gaagcgggcc
     3001 cgatcggggt tggaaaaacg atcaggaatt cctaagaagt tgggcatcga ctcgttgctg
     3061 gaatacaacg acgatcattc cctccgtcaa gtgaaatcct caaaaaagac gtggctggga
     3121 aattgtgttt tgaataagaa ggagagattt aatgaggtgg acgggtcccg aaggagtccg
     3181 tccacctcgg aattttatcg ccatccactt cgggccatta cttaaatgcc accccgtgtg
     3241 gacgagccgg cccggtggct atcgctgtgt tgaagtccaa ccgaaacttg gggtcgagga
     3301 ccaggtcatt ggccgagacc acagcaacgt ggattttgtg atccccctcc gcgtggactt
     3361 tgcccgcgtc atcttcattt aagaagtaat cggtaatcac cagccgcttt tcatccggag
     3421 tgagggcgat gtagtgcggc ccggaa
//
LOCUS       DBNGZD010000369         4013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1791370, whole genome shotgun sequence.
ACCESSION   DBNGZD010000369 DBNGZD010000000
VERSION     DBNGZD010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1791370"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="AC16GM_09710"
     CDS             <1..658
                     /locus_tag="AC16GM_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2923982.1"
                     /translation="TVVMAIFLAAGAFAQPASPIAKGKVLFSVQGTELSVLAVDTLGF
                     AKGLSGDADAGEPSFFPSASLVGGRATVALGLSDQYALNAAFLWAGGFQKTVPADTGE
                     SESKGTITALGVRVGLDRHVPVAKWVSLYGGGGVLWTSTQVKIRVNQGPTSVEYKDPR
                     TSSFSLSGRMGFFVRFKESFGLYGDLGQQLSWVSKKVRGKATALTSSHDGNIGIAVLF
                     "
     gene            763..1350
                     /locus_tag="AC16GM_09715"
     CDS             763..1350
                     /locus_tag="AC16GM_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR2923983.1"
                     /translation="MRKVIMLVLAGAVLGSNVATAKEMKGKFALGLNLGANIPTGDFG
                     NEFKVGGVADLTGEYYLSNVVALGVEVEGSVNDPKSDVAAQFPGVDISATISSFNATG
                     RFFFPSQSVEPYVIAGLGAYTQELDLEVSGFVVKVSETDFGIHGGAGINFPVSPKVGI
                     LAEADFHNIFSKGSSTNFVNVKAGVRLLLGGKKSP"
     gene            1411..1989
                     /locus_tag="AC16GM_09720"
     CDS             1411..1989
                     /locus_tag="AC16GM_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2923984.1"
                     /translation="MRHKFCTVLAIVLLALPGLSWAAHQKGNLGVFVLGTGATGTSDN
                     LKNFTANGGGLGLGAEVYFANHLAFGVQGSATLLPGFQLGGQTTNDWWFWEYGGYLKY
                     AIPALNWFSPYVKGGFGGATITFHESGPTKTPNQTRPVLYAGTGVTLEVSPRWSLFAE
                     ALFHDAMTAGAGPAHWIAGGAGATYWFGLPKP"
     gene            2029..2634
                     /locus_tag="AC16GM_09725"
     CDS             2029..2634
                     /locus_tag="AC16GM_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2923985.1"
                     /translation="MRRFSALTGLATLTLFLASSGWAGYGQGQNAKGQLAFVLRGGAG
                     IPVFDLRTLNIYGQKTGAAFGAQGEYFVKDGLAVGVDVGYTVFSKRTEDNLPRSAVRP
                     WHFLAHLKYYLPVGGKIGPYLEYGAGANHFTFSENVKSQTKFALLAGAGLDIAVRPDF
                     ALVLGGTFDYAIFGKQARDVRSQQVGWFSLLGGVRFTFAAE"
     gene            2821..3735
                     /locus_tag="AC16GM_09730"
     CDS             2821..3735
                     /locus_tag="AC16GM_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR2923986.1"
                     /translation="MFSFSTSWNAGRHSSGRELVAEIRELGFSAIEVNYRVRARALAD
                     IKALVRAGEVSVPSLHNAVPCSEDQDLATAHSAFPLSSPEKKVRQRGIDLARWTLDHA
                     CELGAQAVVLHLGEVEMPGAENLEREYRLFLKDGKTDQAAHFKQKMIAERSRHAAGFF
                     GRALESLHRIALQALTCGVRLGIENRYHWFQVPSFDELQEIFRQFPGETVGYWHDVGH
                     AQTMENTGFTAHLAYLDAFGPRLVGCHLMDCVFDRDHLACGAGEIDFSVIAPYLRDSV
                     LKVFELGQAATPEDIAASVPYLAKFGIQ"
     gene            3825..>4013
                     /locus_tag="AC16GM_09735"
     CDS             3825..>4013
                     /locus_tag="AC16GM_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR2923987.1"
                     /translation="MSGHSKWATIKHKKEKTDAQRGKLFTKLIKELTVAARHGGGDPE
                     ANPRLRTAVAAARAANMPT"
BASE COUNT          785 a         1059 c         1292 g          877 t
ORIGIN      
        1 aacggtagtg atggcaattt tccttgcggc cggggctttc gcccagcccg cgagtccgat
       61 tgccaagggc aaagtgctct tttcagttca gggaaccgaa ctctccgttt tggcggtgga
      121 cacgttaggt tttgctaaag gcttatcggg tgatgccgat gccggtgagc catccttttt
      181 tccgtcggct tccctggttg gcggcagagc gaccgtggcc cttggtctga gtgatcagta
      241 tgcgctcaac gctgccttcc tctgggcggg aggatttcaa aagaccgtgc ctgctgatac
      301 gggagaatca gaatccaaag gcacgatcac ggccctgggc gtgcgggtgg ggctggaccg
      361 gcacgtgccg gtggcgaagt gggtttctct ctacggcggc gggggagtgc tgtggacctc
      421 gacacaggtc aaaatccgcg ttaatcaagg cccaacctcg gtggagtata aagacccgcg
      481 taccagttca ttttcgctct ccggacggat gggttttttc gtccggttca aagagagctt
      541 cgggttgtac ggcgacctgg gacagcaact ctcctgggtt tccaagaagg tgcgggggaa
      601 agccaccgcg ctgacttcca gccacgacgg caacatcggc attgcagtgt tgttctaaag
      661 ttggtatgat acctggcgcc gcgcccggaa agggcacagc gttctcgctt tcacagatct
      721 ccgaggggca gatggcctcg gaaacaggac gggaggcaga ccatgcggaa agtgatcatg
      781 ttggtattgg cgggtgcggt tttgggcagc aatgtggcga ccgccaagga aatgaagggt
      841 aagtttgcct taggactaaa cctgggagcg aacatcccga ccggcgattt cggcaacgag
      901 ttcaaggttg gcggggtggc cgatctcacc ggggagtact atttgagcaa tgttgtggca
      961 ttaggtgtag aggtggaagg gagcgtgaac gatccgaaat cggacgtggc ggcgcagttt
     1021 cccggtgtgg acatttccgc tacgatttcc tcgtttaacg ccactgggcg gttttttttc
     1081 ccctcgcaga gcgtggaacc gtatgtgatt gcgggcttag gggcgtacac acaggaactg
     1141 gatttggagg tatccggctt cgtcgtcaag gtttcagaaa ctgactttgg aattcacggg
     1201 ggcgcgggaa tcaatttccc cgtctcaccc aaagtgggta tcctggcgga agccgatttt
     1261 cacaacatct tctccaaggg gtcgtccacc aatttcgtga acgtcaaagc tggcgtgcgg
     1321 ctgctcctgg gcggaaaaaa gagcccgtaa gtcgatcaaa gcgccgcatt cctctcggga
     1381 tgtagcgcct ctttttcaga aaggagacgg atgcgtcata agttttgcac ggttctggcg
     1441 attgttctct tggcgctacc cggtttgagt tgggcggcgc accagaaggg caatctgggt
     1501 gtgttcgttc tgggcaccgg cgcgacgggg acttccgaca atctgaaaaa ttttacggcc
     1561 aatggcggcg ggctcggcct gggtgccgaa gtgtactttg cgaaccattt agcctttggc
     1621 gtccagggca gcgccacact cctgccgggg tttcagttgg gcgggcagac caccaacgac
     1681 tggtggttct gggagtacgg cggctatctg aagtacgcca ttccggctct aaattggttc
     1741 agcccgtacg tcaaaggcgg tttcggcggc gcgacgatca ctttccacga gtccggtccg
     1801 accaaaacgc ccaatcaaac gcggccggtg ctctacgccg ggacgggcgt gacgcttgag
     1861 gtttcaccgc gctggtcgct tttcgccgag gccttgttcc acgatgcgat gacggccgga
     1921 gccgggccgg cccactggat agccggcggt gccggagcaa cctactggtt tggcttaccg
     1981 aagccgtagt cggctaacag gtatcgggtc ttcctggagg agcgggtcat gagaagattc
     2041 tctgcgctga cggggttggc cacacttaca ttattcctgg cctccagcgg gtgggcgggc
     2101 tacggtcagg ggcagaacgc caaagggcag ctggccttcg tcctgcgggg cggggcggga
     2161 ataccggtct ttgatctccg gacgctcaat atttacgggc agaagacggg agcggcgttc
     2221 ggcgctcaag gagagtattt cgtcaaagac ggtctcgccg tgggcgtcga tgtgggatac
     2281 acggttttct ccaagcgtac tgaagacaat ctgccgcgct cggccgtccg gccgtggcat
     2341 tttctggcgc acctgaagta ctatttgccg gtaggcggga agatcggacc ctacctggag
     2401 tacggcgccg gggcgaatca tttcactttc tcggaaaatg tgaagagtca aacgaaattc
     2461 gcgcttttgg ccggtgccgg tttggacatt gccgtccgcc ccgatttcgc tttggttctg
     2521 ggcggcacct tcgattacgc catcttcggg aagcaggcca gggacgtgcg cagccagcag
     2581 gtgggctggt tcagcctctt gggtggggtg cgctttacgt ttgcggcgga gtaagaatca
     2641 gtagtcaggc tgggaagcct gaccgatgga taggatattc ccccgagtag actttcgaaa
     2701 acgtaccggt tcagtctgaa aacatttccg ccgggcgggt ctttgatccc gcctgtggag
     2761 ggactcgggc taaagagacc ggggctaccc cggatgcgag ttttccggta atttccgcag
     2821 atgttttcat tttcaaccag ctggaatgca gggcggcatt cgtcgggccg ggagctggtt
     2881 gccgaaatcc gggaattagg attttccgca attgaggtca actaccgcgt ccgggcgcgg
     2941 gcgctggccg acataaaagc gctggtgcgt gccggggaag tgagcgttcc cagtttgcac
     3001 aacgccgtac cctgttcgga ggatcaagac ttagccaccg cccacagcgc ctttcccctt
     3061 tcctcaccgg agaagaaggt ccgccagcgc gggattgacc tggcgcggtg gacgcttgac
     3121 cacgcctgcg aactgggagc gcaggcggtg gtgttgcact taggagaggt ggaaatgccg
     3181 ggcgcagaaa acttagaacg cgagtaccgc ctcttcctga aagatgggaa aacggatcag
     3241 gcggcacact ttaagcagaa aatgatcgcg gagcggtcgc ggcacgccgc cggatttttc
     3301 ggcagagcgc tggagtcgct gcaccgtatc gccctgcagg cgctcacctg cggtgttcgg
     3361 ctggggatcg aaaaccgcta ccactggttt caggtgccgt cgtttgatga gctgcaggaa
     3421 atcttccgac agtttcccgg ggagaccgtg ggttattggc acgacgtggg gcatgcgcag
     3481 acaatggaga ataccggctt caccgcgcat cttgcctacc tcgatgcatt cggcccgcgt
     3541 ctcgtcggct gccacctgat ggattgcgtc tttgaccgcg accacctggc ctgcggggcg
     3601 ggcgagatcg atttttcggt gatcgctcct tatttgaggg attctgtcct gaaagttttt
     3661 gaactgggac aagccgccac ccccgaggat attgctgcca gcgttcccta cctggcaaaa
     3721 ttcggcattc agtaggaaat tgcttttctt ggtgtgtttg tatttattat tcacttgcgg
     3781 aacgaaacgt gaagtaagtt ctgtgccaac cagaaggtga ggatatgtcc ggccattcga
     3841 aatgggccac gatcaagcac aaaaaagaga agaccgacgc ccagcgcgga aaactcttta
     3901 ctaaactgat taaggagctc accgtggcgg cgcggcacgg cggaggggat ccggaggcga
     3961 acccgcgact gcgcacggcc gtggcggccg cccgggcggc caatatgccc acg
//
LOCUS       DBNGZD010000370         6746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1238556, whole genome shotgun sequence.
ACCESSION   DBNGZD010000370 DBNGZD010000000
VERSION     DBNGZD010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1238556"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="AC16GM_09740"
     CDS             <1..271
                     /locus_tag="AC16GM_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2923988.1"
                     /translation="FDWTGALGKPVAGTTAWLIDPQESNHFAPSSAPFMVNYRVEDLH
                     ALVKVLKEEGCNVLEKIDESEYGKFAWVIDPEGNKVELWQPPQGQ"
     gene            368..1219
                     /locus_tag="AC16GM_09745"
     CDS             368..1219
                     /locus_tag="AC16GM_09745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR2923989.1"
                     /translation="MQTITLNNGVTMPMGGYGVFQIQDASECTCCVIDAIQVGYRLID
                     TAASYMNEAAVGDGIQQVGLPREQLFVTSKLWVQHAGYERTTQAIHDSLRRLKLDYLD
                     LYLIHQPYGDVHGSWRAMQDAYRAGKLRAIGVSNFQPDRLMDIAAFNDIKPAVNQIEI
                     NPFQQQEESVRFMQELGVQAEAWAPFAEGRNNLFQNEVLRNIAAKHGKTIGQVVLRWL
                     IQRGVVALAKSVRKERMSENLAIFDFELDANDMAQIVKLDTGTSSFFSHRDPAIVKWM
                     SERKLDI"
     gene            complement(1428..1841)
                     /locus_tag="AC16GM_09750"
     CDS             complement(1428..1841)
                     /locus_tag="AC16GM_09750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR2923990.1"
                     /translation="MKASLVWKGEMAFAATSESGHTLVMDASPDQEGDNQGPRPMELL
                     LLSLGGCTGMDVISFLRKKRVTVDELKIAIEAERRKEHPQVFTKVTLDYQIKGKNLKE
                     SDVKWAVELSQEKYCSVIGMLKKTVPITCRWSIST"
     gene            complement(1922..2251)
                     /gene="trxA"
                     /locus_tag="AC16GM_09755"
     CDS             complement(1922..2251)
                     /gene="trxA"
                     /locus_tag="AC16GM_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR2923991.1"
                     /translation="MAKPIEVTDSTFENEVVKSSQPVLVDFWAVWCGPCRMVAPIVEE
                     LAGEYAGRLKVAKMDVDQNNATAARFNILSIPSLLFFKNGQVVDQIVGAVPKKQFVEK
                     INKILGN"
     gene            complement(2329..3606)
                     /locus_tag="AC16GM_09760"
     CDS             complement(2329..3606)
                     /locus_tag="AC16GM_09760"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR2923992.1"
                     /translation="MTYARAVAFLYSLERFGIKLGLDNIRVLLADLGQPQTAFRSIHV
                     AGTNGKGSTCALLASILQQAGFRVGLYTSPHLVDFRERIQVNRKPIPKRWVATFVARH
                     QRRIQRLRATYFEVTTALAFDYFRAAGVDWAVIETGLGGRLDATNVITPAVAVITSIG
                     LEHTEHLGKTITSIAREKAGIIKMGVPVVVGPMPAAALKAIHQVAQAKGSAEVRVRLS
                     QISSMPLPARWQQRFVLEQGGKRRSYTLGFLGSHQIANAACALETLRWLKSKNLPLRD
                     LHVAAGMKKVFWPARFQLLQRRPAVIADVAHNPDGTLALARTWRQFLGKEKAIVVFGC
                     LADKDYRTMLDHLCTFARHFILTAPQTGRARPAADLQTHLRSRRVSHEVAASPVQAYR
                     LALRRSGRTIPVCLTGSHYLVGEIFAHRRVPLW"
     gene            complement(3611..4447)
                     /gene="accD"
                     /locus_tag="AC16GM_09765"
     CDS             complement(3611..4447)
                     /gene="accD"
                     /locus_tag="AC16GM_09765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR2923993.1"
                     /translation="MEWFKKAREGLQSQEKKEIPHGLWTKCEACGEIIYVKELEKNFW
                     VCPKCNYHFRIRSKDYIDLLLDADTFKECDDNLISVDPLRFRDSKRYADRTREARRKT
                     QLNDALVCGIGKIDGREVSFAVMDFAFIGGSMGSVVGEKVARTIERSLAYTYPLVIVS
                     CSGGARMQEGILSLMQMAKTSALLAQISEKKIPYLSVLTNPTTAGVMASYASLGDVIL
                     AEPKALLGFAGPRVIQQTIGQELPEGFQSSEFFLEHGFLDLICERKNIKSTISLLLDY
                     LS"
     gene            complement(4455..4943)
                     /gene="rimI"
                     /locus_tag="AC16GM_09770"
     CDS             complement(4455..4943)
                     /gene="rimI"
                     /locus_tag="AC16GM_09770"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR2923994.1"
                     /translation="MLETLKQPAACSIEPMQSADLAAVAQIERDSFSDPWTPANFSEG
                     LNWEQSFFRVARQDGEVVGYLCTLIAAGEMHIANVAVGESRRGLGIGRALVEEGLNEA
                     RRRGCLAVWLDVRQSNRQARALYEQLGFRRVLVRQKYYRKPVEDAWVYCLVLSPRPGE
                     ET"
     gene            complement(4927..5610)
                     /gene="tsaB"
                     /locus_tag="AC16GM_09775"
     CDS             complement(4927..5610)
                     /gene="tsaB"
                     /locus_tag="AC16GM_09775"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR2923995.1"
                     /translation="MLVLGLDASSAKLSVGLVDDERTLAEATVELQLSRSEVLLQTLE
                     TCLAWAQVKWQDLDGLAVPVGPGSFTGLRIAMATVKGLAYALGKPVVTCTTFEAIASG
                     FRGISRPLVVLFPAPKETVLVAVYESENGMMHPTLAPQICALDNVTMLFPYGALVTGP
                     GAVRYRNRLEKEYGGSFQLTFESDSFVRGVEVARLGLKRLQAGEVADLAWLEPQYFFA
                     WGSGDAGNA"
     gene            complement(5753..6199)
                     /gene="tsaE"
                     /locus_tag="AC16GM_09780"
     CDS             complement(5753..6199)
                     /gene="tsaE"
                     /locus_tag="AC16GM_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR2923996.1"
                     /translation="MTIQLTVVTRKEILSNSLEQTQAYGREFAVGLKSGDVAALSGPL
                     GSGKTAFVRGVCAGLGLTTPVTSPSFIIVNEYPGRLPVYHWDLYRLNSLDEVRRLGIE
                     ECFASEGVCLVEWAEKILSLLPERRWEIHFSIAGENQRKISFQQFS"
     gene            complement(6314..>6746)
                     /locus_tag="AC16GM_09785"
     CDS             complement(6314..>6746)
                     /locus_tag="AC16GM_09785"
                     /inference="COORDINATES: protein motif:HMM:NF020249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HIR2923997.1"
                     /translation="APTESAFRSVMKSWFLHSSLFDVLKYLARQDGVVVITTDHGSIQ
                     AMRGSLVHGKRDTSSSLRYKYGDNLNCDTRQAVLLKDPKTYRLPSFTIATTYVLAKED
                     YYFLYPTNFNEYQQKYQNSFQHGGISMDEMILPVATLKPKK"
BASE COUNT         1486 a         1993 c         1832 g         1435 t
ORIGIN      
        1 cttcgattgg accggcgccc taggcaagcc ggttgcgggc acaaccgcct ggttaatcga
       61 tccgcaagaa agtaatcact ttgctccgag ctctgctccc ttcatggtca actaccgggt
      121 ggaagacctc cacgccttgg tcaaggtctt gaaggaagaa ggctgcaatg tgctcgagaa
      181 gattgacgaa tccgagtatg ggaagtttgc ctgggtcatt gatccagagg gaaacaaagt
      241 cgagctatgg cagccgcctc aaggccaatg accaagctcg ttagtcacgg cagcgaactg
      301 ctgcctaacc agtcgctcaa actgacggta taacgtgccg cgaacccaac atttcgagga
      361 ccgcaccatg caaaccatta cgttgaacaa cggtgtcacc atgccaatgg gtggctacgg
      421 cgttttccag attcaagatg cttctgagtg cacttgttgc gtcatcgatg ccatacaggt
      481 tggatatcgt ctgattgata cggctgcttc ctacatgaac gaagcggccg ttggagacgg
      541 gatacagcaa gttggcctcc cgcgcgaaca gttgttcgtg acgtcaaaac tatgggtgca
      601 gcacgccggc tacgagcgaa caacgcaggc cattcacgat tccttgcgtc gcctgaagct
      661 cgactatctg gacctgtacc ttattcatca gccatatggc gacgtacacg ggtcatggcg
      721 cgccatgcag gatgcctatc gcgccggcaa gttgcgcgcg atcggcgtca gcaatttcca
      781 gccagacaga ttgatggaca tcgctgcatt caatgacatc aagcctgcag tcaatcagat
      841 tgaaatcaat ccgttccagc aacaagagga aagcgtgaga ttcatgcaag aacttggtgt
      901 acaggcggag gcatgggcgc cattcgccga agggcgaaat aatctcttcc aaaatgaggt
      961 gctgcgaaac atcgccgcca agcacgggaa gaccataggc caagtcgtgt tgcgctggct
     1021 aatacaacgc ggtgtcgttg cgctggccaa gtctgttcga aaggaacgca tgtctgaaaa
     1081 ccttgcgatc ttcgatttcg agctggacgc aaacgacatg gctcagattg tgaagctgga
     1141 caccggtacc agtagcttct tctcacatcg tgatcctgcc atcgtcaagt ggatgagtga
     1201 acgcaagctg gatatttgaa atgtttgcgc atccgtgtgc ggtataacaa cgcgttgcag
     1261 ccgacagtcc tcgcgctgtg cgctcggccc gcggctgagc gcgggcgtta ggctggtcag
     1321 ttcgacttcc gggtgatctc gtttgggtac gaaaatgaaa acgccccgtt tcctcggggc
     1381 gcgggggttc aggtatcgat gcgactcccg gtcatatgac tacagactca ggtcgagatc
     1441 gaccagcggc aggtgatggg aaccgtcttc ttgagcatcc cgatcaccga acagtacttc
     1501 tcctgggaga gctcgacagc ccacttgacg tcgctctcct tgagattttt ccccttaatc
     1561 tggtagtcca gagtcacctt cgtgaacacc tgagggtgtt ccttccggcg ttcggcttcg
     1621 atggcgattt tcaattcatc gaccgtcacc cgcttcttgc gcaggaagga aatgacgtcc
     1681 atcccggtgc agccgcccaa agagagcaaa agcagctcca tcggccgcgg gccttggttg
     1741 tcgccctcct gatccggact ggcgtccata acgagcgtgt gccccgattc actggtggcg
     1801 gcaaaagcca tctccccttt ccagaccaga ctcgctttca tgaccggttc ccgctgcgcc
     1861 gctcctgagg cgaaagggaa gtgctttcac cctcagttct caattctgat ttcccacagc
     1921 gttagtttcc taaaattttg ttaatttttt ccacgaactg tttcttggga actgcgccga
     1981 ctatttgatc caccacctgg ccgtttttga agaagagcag gctggggatg gagaggatat
     2041 tgaagcgcgc cgcggtggcg ttgttttgat ccacgtccat tttggcgact ttgagccggc
     2101 ccgcgtactc accggccaac tcttccacaa tcggcgccac catccggcag ggcccgcacc
     2161 agaccgccca gaaatcgact aacactggct ggctcgattt caccacctcg ttttcaaacg
     2221 tgctgtcagt aacttcaatc ggtttggcca tcgatcctcc ttcaagtgta cgcaaaataa
     2281 ggggcgaata tagaccggcc acaggtcggg ggcaagtaat ttctgaccct accagagagg
     2341 cacgcgccgg tgcgcgaaaa tttcgcccac caggtagtgc gacccggtca agcagacggg
     2401 aatggtacgc ccggatcgcc gcaaggccag acgatacgcc tgcaccggcg atgcagccac
     2461 ttcgtgtgac acccgtcgtg agcgaaggtg cgtctgtaaa tctgccgccg ggcgtgcccg
     2521 cccagtctgc ggagcagtca ggatgaagtg ccgggcaaat gtgcacaggt ggtcaagcat
     2581 ggttcggtaa tctttgtccg caagacagcc gaagacaaca atcgcttttt cctttcccag
     2641 aaattgacgc caggttctcg ccagagcgag ggtgccgtcg gggttgtggg ccacatcggc
     2701 gatgaccgcg gggcggcgct gcagcaactg gaaccgcgcc ggccagaaca ctttcttcat
     2761 tccggcggcg acgtggagat cacgcaacgg gaggtttttt gattttaacc agcgcagagt
     2821 ttccagcgca caggccgcgt tggcaatctg gtggctgccc agaaatccga gggtgtagct
     2881 gcgccgtttt cctccttgtt caagaacgaa tctctgttgc cagcgtgctg gtagaggcat
     2941 actcgaaatc tgactcagcc gtacacgcac ttcggcagaa cctttcgctt gagcgacctg
     3001 atgaatggct ttcagagcgg ccgccggcat cggcccgacc accaccggca cacccatctt
     3061 gataattccc gctttttccc gggcaatgga agtaatcgtt ttccccagat gctccgtgtg
     3121 ctccagacct atactcgtaa tgaccgccac tgccggtgtg atgacattgg tggcatccag
     3181 ccgaccgccc aggccggtct cgatcaccgc ccaatcgacg cccgcggcac gaaagtagtc
     3241 gaaggccagt gccgtcgtga cttcaaaata ggtcgcccgc aacctttgaa tccggcgctg
     3301 gtgacgcgcc acgaaagtag cgacccaacg ctttgggatt ggcttacgat ttacctgaat
     3361 tcgctcacgg aaatcgacca gatggggcga cgtgtacaaa ccgacgcgaa aaccggcctg
     3421 ttgcagaatg gacgcgagca gggcgcaggt cgaccctttg ccattggtgc ccgccacgtg
     3481 aatcgagcga aacgcagtct gaggctgccc caggtcagct aacagtacgc ggatgttgtc
     3541 cagcccgagt ttgatgccga aacgttccag cgaatagaga aacgcaacgg cccgggcgta
     3601 ggtcacggag ttatgagagg tagtccagga gaagagatat ggtcgacttg atgttttttc
     3661 gctcgcaaat caaatccaga aaaccgtgct ccaagaaaaa ctccgaactc tggaagccct
     3721 ccggcaattc ctgaccgatg gtttgctgaa tgacccgcgg cccggcaaag cccaaaagcg
     3781 ccttcggctc ggctaagatg acgtcaccta acgaggcgta actcgccatc accccggcgg
     3841 tggtgggatt ggtcaaaacc gacaggtacg gaattttctt ctccgagatt tgcgccagaa
     3901 gcgccgaggt cttggccatc tgcatcaagg agagaatgcc ctcctgcatc cgggccccgc
     3961 cggagcagga gacgattacc agaggatagg tgtaagccag agagcgctca atggtccgcg
     4021 ccaccttttc ccccaccacc gaccccatgc tgccgccgat gaaggcgaaa tccatcaccg
     4081 cgaaactgac ttcccttccg tcgattttac cgatgccgca gacgagggcg tcgttcaact
     4141 gggtcttgcg ccgggcttcc cgggtgcggt cggcgtagcg cttagaatcg cggaagcgga
     4201 gcggatcgac cgagatcaga ttatcatcgc actctttgaa ggtgtcggcg tccaggagca
     4261 gatcaatgta atctttggaa cggatccgga aatgataatt acatttgggg caaacccaaa
     4321 aatttttctc caattcctta acgtaaatga tttccccgca cgcctcgcac ttggtccaca
     4381 agccgtgcgg gatttccttt ttctcctggc tctgcagccc ttcgcgcgct ttcttgaacc
     4441 attccatagc gtgcctacgt ttcctcaccc gggcgaggtg atagtacgag acagtagacc
     4501 caggcatctt ccaccggttt gcggtagtac ttctgccgga ccagcacccg ccgaaaacct
     4561 aactgctcgt agagcgcccg ggcctgccgg ttcgattgcc gtacgtccag ccagacggcc
     4621 aaacaccccc gccgccgcgc ctcgttcaaa ccttcctcga cgagcgcccg gcctattccc
     4681 aaaccccggc ggctctcccc cacggcgacg ttggcaatgt gcatctcccc cgcggcgatg
     4741 agggtacaga ggtagcccac gacttctcca tcctgccggg ctacccggaa aaaactctgc
     4801 tcccagttca gtccttctga gaaattcgcc ggggtccagg gatcggaaaa agagtcgcgc
     4861 tcgatttggg ccaccgccgc caggtcggca gactgcatcg gttcgatgga acaagccgcc
     4921 ggctgcttaa gcgtttccag catcacccga accccaggca aaaaagtatt gcggctccag
     4981 ccaggcgaga tcagcgacct ccccggcttg cagccttttt aaccccagac gggccacctc
     5041 taccccccgc acgaatgaat cgctctcaaa ggtgagttga aaacttccgc cgtattcttt
     5101 ttcgagccgg ttacgatacc gcaccgctcc cgggccggtc accagtgctc cgtagggaaa
     5161 aagcatggtg acgttatcca gcgcgcaaat ctgcggtgcc agcgtggggt gcatcatacc
     5221 gttttcagat tcatagacgg ccaccagcac cgtctctttc ggtgcgggaa agagcacgac
     5281 caaaggccgg gaaatgccgc gaaaaccaga agcgattgct tcaaacgtgg tacaggtcac
     5341 caccggtttt cccagagcat atgccagtcc tttgaccgtg gccatagcga tgcgcagtcc
     5401 cgtgaaagag ccggggccta ccggaaccgc cagtccgtcc aggtcctgcc atttcacctg
     5461 tgcccaggcc aagcaggtct ccagtgtctg gagcaatact tccgatcggg aaagttgcaa
     5521 ctccaccgtg gcttcagcca gcgtccgttc gtcatctacc aaacccaccg aaagtttcgc
     5581 gcttgaagca tctaatccta aaacgagcat gacagtaaca cccactttca atatttcttg
     5641 tgccaaaata taaacatatt tacatcgttc gtaaaagaca aggtttgcct gttatgcccc
     5701 tacgtggggc agcgacggtc gcctgaccgt cttctctttg cctctatttc cctcaggaaa
     5761 attgttgaaa tgaaatcttc ctctggtttt ccccagcgat cgagaagtgg atttcccacc
     5821 tccgctcggg caagagagaa agaattttct ccgcccattc caccaggcaa acgccttccg
     5881 aggcaaagca ctcttcgatc cccaaccggc ggacttcatc aagggaattg agccgataga
     5941 ggtcccagtg gtaaaccggc agccgcccgg gatattcatt gacgatgatg aaactggggc
     6001 tggtgaccgg tgtggtcaat cccagccccg cgcagactcc ccgcacgaac gccgtcttgc
     6061 cgctccccag aggcccgctg agtgctgcca cgtccccgga cttcaggccc accgcaaact
     6121 cccgcccgta cgcttgcgtc tgctccagcg aattcgaaag aatctccttt ctggtaacga
     6181 ccgttagttg aatagtcacg tctttcggac cgtttcttta tttcagcaga ggtataatcg
     6241 ctggcttttg gaaaaggagc aactggtcgg agtcaactcc gacaccccta aacttcgaca
     6301 tctcgacacg cctttatttt tttggcttga gcgtcgccac cggcagaatc atctcgtcca
     6361 tcgaaatccc gccgtgctgg aaggagttct ggtacttctg ctggtactcg ttgaagttgg
     6421 tcgggtaaag gaagtaataa tcttctttgg ccagaacgta cgtggtggcg atggtgaacg
     6481 acggcagccg gtaggttttg gggtctttga ggagcaccgc ctgccgggtg tcgcagttca
     6541 agttgtcgcc gtacttatag cgcagactgg acgaggtgtc gcgcttgccg tgcaccagag
     6601 atccgcgcat cgcctggatc gagccgtggt cggtggtgat gaccaccacc ccgtcctggc
     6661 gggcgaggta cttgagcacg tcgaagagcg acgagtgcag gaaccaggat ttcatcaccg
     6721 agcggaaggc cgactcggtg ggcgcc
//
LOCUS       DBNGZD010000371         3445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1656104, whole genome shotgun sequence.
ACCESSION   DBNGZD010000371 DBNGZD010000000
VERSION     DBNGZD010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1656104"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1230
                     /locus_tag="AC16GM_09790"
     CDS             160..1230
                     /locus_tag="AC16GM_09790"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR2923998.1"
                     /translation="MSALWALPLSFSYPATTATVQADTLPPPIPPKPPRPPKKKKEIQ
                     RRGYVLGESLFIEPQPPAAVPGYRNQKGTAFALAGEITVAEDEEATGDVVVLFGSIEV
                     FGRVHGNVVSVMGNVHLGPRAVVDGDVVAANLAVEPGAQIHGDRVEIPFPNMGFPFGR
                     DFFRHGGGLLLAVILLTISSLFGILTLAVAPRQVAKVNQKIDSGLFKSFFVGFVGEIL
                     LLPIFILLCVTVIGIPVAFLIFPHLVVAAYLLGNTAISLSVGQKLRQGTQSPGASSMI
                     TMLSGKFFLYLPFLCAGFLGLFGGGFGPLFRTFSLIFFLFGLALHFAATASGFGAALL
                     SRYGTRPKEPPPPPPAAHMQPT"
     gene            complement(1300..>3445)
                     /locus_tag="AC16GM_09795"
     CDS             complement(1300..>3445)
                     /locus_tag="AC16GM_09795"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR2923999.1"
                     /translation="RIVEGKFQIGKELFSPHSVEVHYFRLSKRYWSVCFERIKRIGFR
                     ILSTAVPWNLHEDVRGGFDFSGTVDSRKDLIVFLELAREFGFKVILRPGPWIGAEWPN
                     GGYPDYLINMPELLARDAQGNPVPNRLRAGVKAGFVPSYHHPRYQSAVKRYFQALGDT
                     IKHYVYPRGPIFLFELDHQASFGGNLDPFAADYNQFTLSTLYPQFLERKYQETKRLNQ
                     TYGERHAAFTAVEPPRSVPGKRGLGYPKFFDWIAFRQFLLQDYVAGLKESLKQVDLSA
                     LFYFTQPALGSRLCLPPLAGSESGEVFSGVSLPDGDDFSGAAALLRQAAGQRQFAWVS
                     DLPLGRAAADPEQTKKHLPVHAADIKRTVVQAAASGVQGINFNSFVERDHWYGAPLAA
                     DGTVQPAYEILQKFTLAHEKFPWQPTSLSHSVGLAAYQPYLWLEKTLGPANPGQNWFT
                     SRLQEGISRDLEYLKVGFGEVETTAASELERYSLFILAVAEFMALETQEQLLRLIQSG
                     KIGLFVGTLPRVDLAGKSCTLLAKGLRTSTAPFSGVERVKGFGQEFSAALFGTIRFSR
                     LKKICTAGGRTVGAGGKVGKGSAFVLTFDPVSHLQPHKLAFWEALFRSLGIARPVATS
                     DPQVIVSLHQNEGRTLAAVLLPPAAAPAGTRRQIIFQMDTRALGIRGDRLRLADLLGQ
                     VTLRTSASELRRGIVLEIAPGDSFLFLIDKRS"
BASE COUNT          678 a         1038 c         1023 g          706 t
ORIGIN      
        1 aagccaccgg ctggaaatga aaagtacatc cggcgacatt gccgtggcca gtttctaaag
       61 gacgaacgcc atgtcggaat tttggaaaaa tctgattctc gacaatgacc ggggggattc
      121 gtgaatcggc ggactgcacg ctggctactg cttttcgttt tgagtgctct ttgggccctg
      181 cctctctctt tcagttaccc ggccacgacg gccaccgtgc aggccgatac cttgccaccc
      241 cccatccctc caaaacctcc ccgcccgccg aaaaagaaaa aggaaatcca gaggcgcggg
      301 tacgtcttag gtgaaagtct tttcatcgaa ccgcagccgc cggccgcagt gccggggtac
      361 cggaaccaaa aagggaccgc ctttgccctg gccggagaaa tcacggtagc cgaagacgaa
      421 gaggccaccg gagatgtcgt ggttctcttc ggcagtattg aagtcttcgg ccgggtgcac
      481 ggcaacgtcg tgagcgtcat gggcaacgtc catctggggc cccgggccgt cgtggacggc
      541 gacgtggtgg ccgccaacct cgccgtggag ccgggggcgc agattcacgg cgaccgggtg
      601 gaaatcccct ttccgaacat ggggttccct ttcggccggg attttttccg gcacggcggc
      661 ggactgctcc tggcggtcat tctcctgacg atttcgtcgc tgttcggcat cctcaccttg
      721 gcggtagccc cccgtcaggt cgccaaagta aatcagaaga ttgactccgg gctcttcaaa
      781 tcattcttcg tgggctttgt gggggaaatt ctgcttttgc cgattttcat tttgctgtgc
      841 gtcacggtaa taggaattcc cgtggccttc ctaatctttc cccatttggt cgtcgcggcg
      901 tacctcttgg ggaatacggc catttcgctc tcggtcggtc agaaactgcg ccagggcacg
      961 cagtccccgg gcgcttcgtc catgatcacg atgttgagcg ggaaattctt cctctacctg
     1021 ccgttccttt gcgccggttt tctggggctc ttcggggggg gcttcggtcc cctcttccgc
     1081 accttcagtt taatcttctt tttattcggc ctggctctgc acttcgcggc taccgcctca
     1141 ggtttcggtg cggcgctgct ctcccgctac ggcacccggc ccaaagaacc accccctcct
     1201 cctccggctg cgcacatgca accgacgtaa gccgtagcgc ccccgcaccg cagcggcgct
     1261 tctgcatttc tctctgggga agattttccg agatgctccc tacgagcgtt tgtcgatcaa
     1321 aaagaggaac gaatcgcccg gggcgatttc caaaacgatt ccccgccgga gttcagacgc
     1381 cgaagttctc agagtcacct gacccaatag gtctgccagc cgcaggcggt ctccccgaat
     1441 ccccagcgct cgggtgtcca tctggaagat gatctggcgg cgggtgcccg ccggggctgc
     1501 ggcaggcggg aggagcaccg cggccagcgt gcgcccttcg ttctggtgga gcgaaacgat
     1561 cacctgcggg tcgctggtgg caaccggacg ggcaatcccc agcgagcgga agagagcttc
     1621 ccagaacgcc agtttgtgcg gctgcaggtg gctcacggga tcgaaggtca aaacgaaggc
     1681 gctccccttg cccactttcc cgccggcacc cacggtgcgc ccgccagccg tacagatttt
     1741 tttcaggcgg gagaagcgaa tggtgccgaa gagggccgcg gaaaactcct gcccgaaacc
     1801 tttgacccgc tcgaccccgg aaaaaggcgc ggtgctcgtc cgcagacctt tggccagaag
     1861 cgtgcaggat tttccggcca aatccacccg cggcagggtc ccgacgaaca ggccgatttt
     1921 ccccgactga atcaggcgta agagctgctc ctgcgtctcc agagccatga actcggccac
     1981 cgcgaggata aagagagagt acctttccag ttcgctggcc gcggtggtct cgacctcgcc
     2041 gaacccgact ttcaggtact ccagatcgcg gctgattcct tcctgaaggc gggaggtgaa
     2101 ccagttctga cctggattag ccgggcccag tgttttctcc aaccacaggt acggctggta
     2161 ggctgccaga ccgacggagt gggaaagact cgtcggctgc cagggaaatt tctcgtgggc
     2221 cagggtaaac ttttgtaaga tttcgtaggc gggctggacg gtgccgtcgg cggccaaagg
     2281 cgccccgtac cagtggtctc gttccacaaa cgagttgaaa tttatcccct gaacgccgct
     2341 ggccgccgct tgcaccaccg tccgtttgat gtcggcggcg tgtaccggca aatgtttttt
     2401 cgtctgctcc ggatcggcgg ctgcgcggcc gagcggcagg tcgctgaccc aggcgaactg
     2461 gcgctgtccg gctgcctgac ggaggagtgc cgccgcaccg gagaaatcgt ctccgtcggg
     2521 cagactcact ccggaaaaga cttccccgga ctcgctgcca gccaagggcg gcaggcaaag
     2581 ccggctcccg agcgcaggct gggtgaaata gaagagggcc gacagatcga cctgcttcaa
     2641 gctctccttc agccccgcga cgtaatcctg gaggagaaac tgacgaaagg cgatccagtc
     2701 gaaaaatttc ggatacccca gaccgcgttt gcccggcacc gaacggggcg gttccacggc
     2761 ggtaaaagca gcgtggcgct caccgtacgt ctgattgagg cgtttcgtct cctggtactt
     2821 gcgttccaga aactgaggat agagagttga aagcgtgaac tggttgtagt cggccgcaaa
     2881 cggatcaagg tttccgccga acgaggcctg gtggtccagt tcgaagagga agatcggccc
     2941 gcgcgggtag acgtagtgct tgatcgtatc gcctaaggct tggaaataac gcttcactgc
     3001 cgattggtac cgggggtgat gataggaggg gacaaaaccg gctttgacgc cagcccgcaa
     3061 gcggtttggg accggattgc cttgggcgtc cctggccaaa agttccggca tgtttattaa
     3121 gtagtcgggg taccccccgt tgggccattc ggctccaatc cagggtcctg ggcgcaaaat
     3181 cactttgaaa ccgaattcgc gcgccagttc gaggaaaacg atcaaatctt tgcgggaatc
     3241 aacggtcccg ctgaaatcaa acccgccgcg cacgtcttca tggagattcc agggaacggc
     3301 tgttgacaat atcctgaaac cgattcgttt gattctttca aaacagaccg accaataccg
     3361 tttgctcagc cgaaagtaat gtacttcaac ggaatggggg gaaaagagct ccttgccgat
     3421 ctgaaacttc ccttcgacaa tccgg
//
LOCUS       DBNGZD010000372         7219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_128164, whole genome shotgun sequence.
ACCESSION   DBNGZD010000372 DBNGZD010000000
VERSION     DBNGZD010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_128164"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="AC16GM_09800"
     CDS             complement(<1..377)
                     /locus_tag="AC16GM_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924000.1"
                     /translation="MLTAKRRLRGVRLLEPSPAYAHLSAATAAEVMQPIFQDELSGAW
                     NRPVRLGKFFIPRVIPIGGGKFLIQYRFSTVEATGPRDWIFFGRILAPDETVPTFVQK
                     PNAFYLAQFRLVVPIFPFDPKLKV"
     gene            complement(453..2318)
                     /locus_tag="AC16GM_09805"
     CDS             complement(453..2318)
                     /locus_tag="AC16GM_09805"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2924001.1"
                     /translation="MDLKFRIPLAKVDWVGLGRIFRHFGKHLGPHKKSLFWGGASLLG
                     LMLVELARPWSLKIVIDYILIPSKAKRDFGFLAPLSHWDPMAVLTFAAGSVAVLSLGG
                     ALFSYAQNVLFASVGEKVSGAIRLELFSHIQRLPQSYHDYRETGEVLMRLTGDIDLLK
                     ELLVSLFISLGSRIFIVVGMLALMLYMNWELTLIAIIVLPLLFLTSLRFSVKLKEASR
                     RQRKKEGQLAAAVYEGVSGISLTKIFGQEKRHDKLLGKSVSSDVKAGLRTTKLESAYA
                     RWVDVITALGVMLVLFFGVKQVQAGHLSAGNLLMFLSYLRSAYKPLRDVAQLSSRTAK
                     ATVCGERVLEILAMQPEVLDKPEAVPAQDIQGEIRFANVGFSYQTGRPVLSDLSFRLP
                     AGQTTAIIGPSGAGKSTIAKLLLRLYEPEAGTIFVDGVPIGDYQIKSFRKRITSLSQE
                     AFLFRTTIRDNISFGKPKASFDEIVAAASKVGADEFIRALPQGYDTLVGEGGQTLSGG
                     QRQRITFARAGLRNSRIMIFDEPATGLDPQAEKVAQEALATLKAGRTLLLITHRLNFL
                     NLADRIVFLEDGRLVEAGTPAELLARKGQFFAFYQEWLSQNEKRGFFVPLAVAAG"
     gene            complement(2327..3532)
                     /locus_tag="AC16GM_09810"
     CDS             complement(2327..3532)
                     /locus_tag="AC16GM_09810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2924002.1"
                     /translation="MKILYLSFDPGIPYGGTKGSSIHIREFTKALRQAGHQVTTVVAR
                     LGAAAPKAGQVYKLPRVEADFFRPEAAWGSPALLAEAKAFAQNFAAKKLLKKLPAAHF
                     DFIYERYSLFGIAGLAWAREQQLPFLLEVNSPLLREAKTHRQLVLEPVARAVEQYLFS
                     QADHIIAVSLAMKEYIGSVAPEARHRRVTIVPNGVDVKPFLRPGSSVGAKAKREKGEF
                     RVGFVGSLKPWHGLEFLLEAFCRLPAREKLKLVIIGEGPLRHQLEKEAGKLGLQDRVM
                     FTGAVRFEQIPRTLKSLDAFVAPYPQIDGFYFSPLKIFEYMAAGRPIVASRIGQVAEI
                     LHDGKNALLVPPEDPAALVSALRRLKADRKLGARLGREAQRAALREHTWQVRLATLEP
                     VFHSLAAKR"
     gene            complement(3529..4812)
                     /locus_tag="AC16GM_09815"
     CDS             complement(3529..4812)
                     /locus_tag="AC16GM_09815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2924003.1"
                     /translation="MNPLRVGYFVKMYPRLSETFILNEILELERRGVEVIIFALRKPN
                     EGRFHPQVAEIKAPVFYLEELSPKKGWAVLAEHWTTLAPHSLQLWRLMEEVIGRDDPK
                     LLEALLAAAWATAVAVERGLGHLHAHFAVVPSTLAYFASRIAGLPFSFTAHAKDIFEN
                     TVDHQLLEEKLQAARFTITVTEFNRRYLTATYPAVPPDKIRVLPNGINLDFFNFEPNV
                     PRERSLILSIGRLVPKKGFPDLLEACAILKGKKIPFYSVIIGQGEDAPLLEAKRQELG
                     LTDVVTFVGPKTQVEVLPYLKRAAVLALPCTVDTDGNQDALPTVLLEALGTGCPVVST
                     TVSGIPEIIDSGSDGLLVPPATPEALAQALERLLLSPELAARFARQGRKKAEEKFDLK
                     KSVATLESYYRRSVETGTAPAAAEISLPVFGYKQS"
     gene            complement(4809..6074)
                     /locus_tag="AC16GM_09820"
     CDS             complement(4809..6074)
                     /locus_tag="AC16GM_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR2924004.1"
                     /translation="MKVKMAAPPFPAIPAERRLPSELARIVIYSHDTFGLGHFRRCLK
                     IARGFKSTYPQASILLVTGQPQALRYVLPEGTDFIKLPAVVKTGDNCYEPRSLSGSFE
                     SIFVLRQNLIFEAVRTFNPHLFLVDHSPLGMKGEIQKTLLWLKGERRRTAVVLGLRDI
                     LDEPEKVCATWRQESVYPILDETYDRIFIYGSPEVFDPVSAYQFGERARAKTSFTGYI
                     TDGARLSAGASEHRKGNGRKEVLVTIGGGEDGADIVTAYLEMLSRYPDQFNAQTTIVT
                     GPFLPEPQQKIFSQKASGLPVHLEEFIPELFPYLQRADLVIAMGGYNTVTEILAAARR
                     ALLIPRTHPRKEQLLRAQRLSELGLVSYIVPDRLTPDALYGTVTALLESDGEPLAQAR
                     RKNLLPLDGAPKLAQSCRSLLQRSAEVPR"
     gene            complement(6071..>7219)
                     /locus_tag="AC16GM_09825"
     CDS             complement(6071..>7219)
                     /locus_tag="AC16GM_09825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR2924005.1"
                     /translation="RANLSRQRLAVGAHMAEAVKGSRAIFLAVGTPALPSGEADLSAV
                     FEVMQNIAQTLEPKQVIVLKSTVPVGTAAQVRRVLAENGAREKQIAVVSNPEFLREGT
                     AVYDFFRPQRIVIGGNSPEALELVAQLYRIGMVEPCPIVVTNNETAEMIKYASNLFLA
                     TKVSFVNELALLCDAAGVNVTEVTRGMGLDSRISPEFLNPGPGFGGSCLPKDLEAILS
                     LAKKHGIDLFIAQAVRQANLRQPEKVVEKLEQLVGSLQDKTIGVLGLAFKAHTNDLRD
                     SPALAVMKLLKQKGAILQAYDPIATLDGKPFGQEMRQVVSAAAAAAGAHALVVLTEWP
                     EFLALDWEALGEKMKARNIVDARNLFVPEVVVRHGFTYVGMGQKPEEV"
BASE COUNT         1475 a         2077 c         2260 g         1407 t
ORIGIN      
        1 actttgagct tgggatcaaa ggggaagatg ggcacgacga ggcggaactg cgccaggtag
       61 aacgcgttgg gcttttggac gaaggtgggc accgtttcgt ccggggccag aatccggccg
      121 aagaaaatcc agtctctggg gccggtggct tcaaccgtgg agaaacggta ctggatcaaa
      181 aattttccgc cgccgatggg aatgacgcgc gggatgaaaa acttccccag ccgtaccggc
      241 cgattccagg ccccggataa ctcgtcctga aagatcggtt gcatgacctc cgcagcggtt
      301 gccgcgctca gatgggcgta cgccggactg ggttccagca ggcgtacgcc gcgcaggcgg
      361 cgtttcgcgg ttagcatatg accgcgctcc ccgctcgggg aaaccgaccg ggaaaagcac
      421 ccatcgggcg gaccggaaga gcgggtagcg agctacccag cggcgaccgc caggggtaca
      481 aaaaaacctc ttttttcgtt ctgggagagc cactcctggt agaacgcaaa aaattgtcct
      541 ttccgggcca aaagctccgc gggtgtgccg gcttcgacca gtctgccgtc ttccagaaaa
      601 actatccggt cggccagatt taagaagttc agtcggtggg taatcaaaag cagggtgcgt
      661 ccggccttga gcgtcgccag cgcttcctgg gctactttct cggcctgggg gtccaggccg
      721 gtggcgggct cgtcaaaaat catgatccgg gagttgcgca gcccggcccg cgcgaacgta
      781 atccgctgcc gttgtccgcc cgaaagggtc tgcccgcctt ccccgaccag ggtgtcgtac
      841 ccctgcggca acgcccggat gaactcatcg gcccccacct tgctggcggc ggcgacaatt
      901 tcgtcgaaac tcgccttggg tttgccgaag gagatgttgt cgcggatcgt ggtgcggaac
      961 aggaaggcct cctgggagag cgaggtaatc cgcttgcgaa acgatttaat ctggtagtcg
     1021 ccaatcggca cgccgtcaac gaaaatcgtt ccggcctccg gctcgtagag gcgtaagagc
     1081 agtttggcga tggtcgattt cccggcaccg ctggggccga tgatggcggt tgtttgaccg
     1141 gcggggaggc ggaaagagag atcggagagc accggccggc cggtttggta ggaaaaaccg
     1201 acgttggcaa aacggatttc cccttgaatg tcctgcgcgg gaaccgcctc cggcttgtcc
     1261 agcacttccg gctgcatggc cagaatttcc agaacccgtt cgccgcacac cgtggctttg
     1321 gcggtccggg acgagagctg ggctacatcc cgcagcggct tgtacgcgct ccgcaaatag
     1381 gagaggaaca tgagcagatt gccggccgag aggtggccgg cctggacctg cttgaccccg
     1441 aagaagagga ctaacatcac tccgagcgcg gtgatcacgt ctacccagcg ggcataggcc
     1501 gactccagct tggtggtccg caggccggct ttgacgtcgg aggagactga tttgcccaaa
     1561 agcttgtcgt ggcgtttttc ctgaccgaaa attttggtca ggctgatccc ggaaactccc
     1621 tcgtagacag cggcggcgag ctggccttct tttttgcgct ggcgccggga ggcctctttc
     1681 agttttacgg aaaaccggag gctggtgagg aacaggagcg gcagcacgat gatcgcgatc
     1741 agggtcaatt cccagttcat gtagagcatg agggcgagca tgccgacgac gatgaagatc
     1801 cggctgccca aggagatgaa gagcgagacc aaaagctctt tgagcaaatc aatatcgccg
     1861 gtcaggcgca tcagcacttc acccgtctcc cggtagtcgt ggtagctctg ggggaggcgc
     1921 tggatgtggg agaacagctc cagacgaatg gctcctgaaa ctttttcccc gaccgaggcg
     1981 aacaggacat tctgagcgta ggagaagagg gccccgccga gtgagaggac cgccacgctc
     2041 ccggcggcaa atgtgagaac ggccatgggg tcccagtggg agaggggtgc cagaaaaccg
     2101 aaatcgcgtt tggctttgga gggaatcagg atgtaatcaa tgacaatttt caaagaccag
     2161 gggcgcgcca gctccaccag catcagcccc agaagggaag cgccgcccca gaagaggctt
     2221 ttcttatggg gccccaaatg ttttccgaag tgccggaaaa tgcggccgag ccccacccaa
     2281 tctactttcg ccagaggtat gcggaatttc aggtccatca accgacctat cttttggccg
     2341 ccagggaatg gaaaactggt tcgagggtgg ccagccgtac ctgccaggtg tgctctctta
     2401 aggcggcccg ctgcgcttcg cgccccagac gcgctcccaa tttccggtcg gccttcagac
     2461 ggcgaagagc cgagaccaga gccgccgggt cttcgggcgg aacgagcaga gcgttcttgc
     2521 catcgtgcag aatttcggcc acctggccga tgcgggacgc cacgatcgga cgaccggcgg
     2581 ccatgtactc aaaaattttg agcggcgaaa aatagaagcc gtcgatctgc ggatagggag
     2641 cgacgaacgc gtccagactc tttaacgttc ggggaatctg ttcaaagcgc accgcgcccg
     2701 tgaacatcac ccggtcctgc aagcccagct tgccggcctc tttttcaagc tggtgccgca
     2761 gcgggccttc accgataatg accagtttga gcttttctct ggccggcagg cggcagaacg
     2821 cctccagaag aaattccaga ccgtgccacg gtttcaggct gccgacaaag ccgacccgga
     2881 actctccttt ctcccgcttt gccttggcgc ccacagacga accaggcctg aggaagggtt
     2941 tgacgtcaac cccgttgggc acaatggtta cccgcctgtg gcgggcctcc ggggcgacgg
     3001 agccaatgta ctctttcatc gctagagaaa cagcgatgat atggtcggcc tgggaaaaaa
     3061 ggtactgttc cacagctctg gccaccggct ccagaaccag ttgccggtgc gttttggcct
     3121 cccggagcaa aggggaattt acctccaaaa ggaagggaag ctgctgttcc cgggcccacg
     3181 cgaggccggc tatgccaaac agggagtagc gctcgtaaat gaaatcaaaa tgcgcggccg
     3241 ggagtttttt caaaagtttt tttgcggcga agttttgcgc aaaggccttg gcttcggcca
     3301 gaagagcggg tgagccccag gccgcctccg ggcggaaaaa gtcggcttcg actctgggaa
     3361 gtttatagac ctgaccggct ttgggagcag ctgctcccag ccgggcgacg accgtggtta
     3421 cctgatgccc ggcttgccgc aaggccttgg tgaactcgcg gatatgaatc gagctgcctt
     3481 tggtgccccc ataggggatg ccgggatcaa acgacaggta cagaatcttc atgactgttt
     3541 ataaccgaaa accggcaaag agatttccgc tgcggcgggc gccgttcccg tttccaccga
     3601 ccgacggtag tagctctcca gtgtggcgac acttttcttc aggtcaaatt tttcttcggc
     3661 tttcttcctt ccctgccggg cgaagcgggc ggccagttcc ggagagagca aaagcctttc
     3721 cagcgcctgg gccagcgcct ccggggtggc cggcgggacc aggaggccat cgctgccgga
     3781 atcgataatt tccggaatgc cggagacggt ggtggaaacc accgggcaac ccgtgcccag
     3841 ggcctctaag agtacggtag gcagggcatc ctgattgccg tcggtgtcca ccgtgcaggg
     3901 cagggcgagc accgctgccc gcttcaggta cggcaggact tcgacctggg ttttcgggcc
     3961 gacgaaggtc accacatcgg tgaggccgag ctcctgccgt ttggcttcga ggaggggagc
     4021 gtcttcaccc tgcccgataa tgacggaata gaagggtatc tttttccctt tcagaatggc
     4081 gcaggcctcc agtaaatcgg gaaaaccttt tttgggcacc agccggccga tggagagaat
     4141 caagctccgt tcgcgaggca cgttgggctc aaaattgaag aagtccaggt tgatgccgtt
     4201 gggcagcact ctgattttgt ccggcggcac ggccggatat gtggcggtca ggtagcggcg
     4261 gttgaattcc gtcacggtga tggtgaagcg ggcggcctgt aacttttcct ccagcagctg
     4321 gtggtccacc gtattctcaa aaatatcttt ggcgtgggcc gtgaagctga acggcaaccc
     4381 agcaatgcgg ctggcgaaat aggccagggt ggagggcaca acggcaaagt gcgcgtgcag
     4441 gtgtccgagc ccccgctcta cagcgaccgc ggtggcccag gcggcggcta agagcgcctc
     4501 gagcaacttg ggatcgtccc ggccgatcac ctcctccatg agccgccaga gctgcaaact
     4561 gtgcggagcc agggtcgtcc agtgctcggc taacaccgcc cagccttttt tgggagagag
     4621 ttcttccaga tagaagaccg gggccttgat ctccgccacc tggggatgga aacgaccctc
     4681 gttcggcttc cgcagcgcga aaatgattac ctccacgccg cgccgctcca gttccagaat
     4741 ttcgttcagg atgaacgttt ccgacagacg ggggtacatt ttgacaaaat agccaacgcg
     4801 taacggattc atcgtggcac ctcggcagaa cgttgcagaa gggaacggca cgactgggcc
     4861 agttttggtg caccgtccag cggcagaaga ttttttcggc gggcctgggc caagggctca
     4921 ccgtcacttt ctaagagcgc cgtgacggtt ccatacagag cgtcgggcgt cagccggtca
     4981 ggaacaatgt agctcaccag acccagttcg gacagtctct gcgcccgcag gagctgctcc
     5041 ttgcgggggt gggtacgggg gatgagcaag gctcggcgag cggcggccag aatttcggtc
     5101 acggtgttgt agccgcccat ggcgatgacc agatcggctc tctggaggta aggaaaaagc
     5161 tccggaatga attcctccag gtggaccggc agtccactag ctttttggct gaaaattttt
     5221 tgctgcggct cgggcagaaa aggaccggtg acgatcgtgg tctgggcgtt gaactggtct
     5281 ggatagcggg agagcatctc caggtaggcg gtgacgatgt cggcgccatc ttcccccccg
     5341 ccgatcgtca ctagtacttc cttccgtccg tttccctttc tgtgctcgga cgcgccggcg
     5401 gacagccgcg cgccgtcggt aatgtagccg gtaaagctcg tcttggcccg ggctctttca
     5461 ccgaactggt aggcggagac cgggtcaaaa acttccggtg acccgtagat gaaaatccgg
     5521 tcgtaggtct catccagaat cgggtagacg ctctcctgcc gccaggtggc gcacaccttt
     5581 tccggctcgt ccaagatgtc gcgcagaccc aagacgaccg cagtccgccg cctttcccct
     5641 ttcagccaga ggagcgtctt ctggatttcc cctttcatgc ccaaaggcga atggtcgacc
     5701 aaaaagaggt ggggattgaa cgtgcgcacc gcctcaaaaa tcaaattctg ccgcaggacg
     5761 aaaatgctct cgaaagagcc ggaaagcgag cggggttcgt agcagttgtc cccggttttg
     5821 accaccgccg ggagtttgat gaaatcggtt ccctccggca gtacgtagcg cagtgcctgc
     5881 ggctgtccgg tgactaggag aatcgatgcc tgcggatagg tacttttgaa accccgggcg
     5941 attttcaaac agcggcggaa gtgccccagc ccaaaggtgt cgtgggagta aatgacgatc
     6001 cgggccagct ccgacgggag tctgcgctcg gcaggaattg ccggaaaggg gggggcggcc
     6061 atcttgactt tcatacttct tccggttttt gccccatgcc cacataggtg aagccgtggc
     6121 ggacgactac ttccggcacg aagagatttc gggcatcgac gatgtttctg gctttcatct
     6181 tttccccgag cgcctcccag tccagagcca gaaattcggg ccattcggtc aaaacgacta
     6241 gggcgtgcgc cccggcggcg gcggcggcgg cggaaacaac ctgtcgcatt tcctggccga
     6301 agggcttccc atccagagtg gcgatcgggt cgtaggcctg gagaatggct cccttctgct
     6361 tgagtagttt cataaccgcc agcgctggag agtcgcgtag gtcgttggtg tgggctttga
     6421 aagccaggcc caggacgccg atcgttttgt cctgcagact gccaaccagt tgttccagtt
     6481 tctccaccac tttttccggc tggcgcaagt tggcctgccg gaccgcctgg gcgatgaaaa
     6541 gatcgatgcc gtgctttttg gctaaactga gaatcgcttc cagatctttg gggagacagg
     6601 atccgccgaa accggggccg ggattgagga actccgggct gatgcgggag tccagcccca
     6661 tcccgcgggt cacttcggtg acgttcacac cggcggcatc gcagagtagc gccagttcgt
     6721 tgacgaagga gactttggtg gccaaaaaaa gattggaagc gtacttgatc atttccgcgg
     6781 tttcgttgtt ggtgacgacg atcgggcagg gctccaccat cccgatccgg tagagctggg
     6841 cgaccaactc caaggcctcc ggactgttgc cgccgatgac gatccgctgc gggcggaaga
     6901 agtcgtagac cgcggttccc tcgcgcaaaa attccgggtt ggaaacgacc gcaatctgct
     6961 tctccctcgc tccgttttcg gcgaggacgc ggcggacctg cgccgcggtg ccaaccggca
     7021 cggttgattt caaaacaatc acctgcttcg gctccagagt ctgggcgata ttttgcatca
     7081 cttcaaaaac cgccgacaaa tcggcttcac ccgaaggcaa cgcgggagta ccaaccgcca
     7141 gaaagatcgc gcgcgagccc ttgaccgctt cggccatgtg ggcaccgacg gccagccgtt
     7201 gccgggaaag gttggcccg
//
LOCUS       DBNGZD010000373         6795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_682295, whole genome shotgun sequence.
ACCESSION   DBNGZD010000373 DBNGZD010000000
VERSION     DBNGZD010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_682295"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="AC16GM_09830"
     CDS             <1..681
                     /locus_tag="AC16GM_09830"
                     /inference="COORDINATES: protein motif:HMM:NF025179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HIR2924006.1"
                     /translation="RRDLAGAEDSLKAFQKLHKTVLLDEETKAAVKSAAELKAQITTD
                     EIQLAVLKKTMGSDHPEVQKLTQKIEETRRQLAKIEFGGARSDPKGYLSIPFEKFPDV
                     SLQLAQLMRDLAVQEKVLELLTQQDEQAKIQEKKDTPTVMVLDPAVPGSKVRPRRSLL
                     VVLVGGLSLVASIFYILAAEYFRKLRFVRPEEYSRVAEIKTAVVHDLPLVGRLFNRTK
                     APFTEGDR"
     gene            678..1697
                     /locus_tag="AC16GM_09835"
     CDS             678..1697
                     /locus_tag="AC16GM_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR2924007.1"
                     /translation="MKPVNVAVIGIGAWGKNLLRNFASLKGCAVTVGCDADAKNKATV
                     TNLAPTATFTTDFRQALDADSVEAVVIATPPASHFAIARAALEAGKDVFVEKPLTLKV
                     GDGEALVRLAEEQQRILMVGHIMEYHPAVLKLKGYVTSGELGKIYYLYASRVNLGKVR
                     DTENALWSFAPHDISLVLFLLEQKPVKVTANGAAYLKPGIPDVAFLSLLFADGVLAHI
                     HVSWLDPHKDRKLTVVGSRKMAVFNDAEASEKIWIYDKGVDTKLDYNTYAEFLSLRTG
                     DITIPKVDGGEPLAVECQHFLDCVAKRTQPRSDGQDGLTVLRVLDAAQRSLDQGGTPV
                     EVRYA"
     gene            1708..2451
                     /locus_tag="AC16GM_09840"
     CDS             1708..2451
                     /locus_tag="AC16GM_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR2924008.1"
                     /translation="MQPVIATSAKVGKKIRWGQFCVVGEGVTIGDGCLIGNHVTIHNG
                     TVIGNNVRIDDNTVIGKQPMRAANSAVTKEQELPPAAIGDDCIIGTSVVIYAGCKLGR
                     KILVADLSTVRENVTVGDFTIIGRGVAIENFCTIGSYCKLETNVYITAYSQIGDRVFV
                     APCVATSNDNFVGRTKERFKHFKGVTVKTGGRIGTNATILPGKVIAEDALVAAGSVVT
                     KDAPARKIVMGVPARVLRDVPKEQLLENQ"
     gene            2608..3711
                     /locus_tag="AC16GM_09845"
     CDS             2608..3711
                     /locus_tag="AC16GM_09845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003351945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR2924009.1"
                     /translation="MKVPLLDLKRQYQAIKSEIDAAVMKVLAETQFILGPEVKEFEQT
                     IAQYLGVKHAIGVASGTDALLLSLRVCGVGPGDEVVTSTFSFFASAGVISRLGATPVF
                     ADIDPKTYNLDPKSIAAKITKKTKVIMPVHLYGQCAEMDPILELDRSHKLDVVEDAAQ
                     AIGATYRGRKAGTMGRFGCFSFFPSKNLGGMGDGGLIVTNSDDDAELLRRLRVHGSKP
                     KYYHSVVGYNSRLDTIQAAVLQVKLKYLDAWTAARREKVKFYNRGLAGSPVETPFEPE
                     YNHHIYHQYTLALRDRDGLKARFAERGIGCDTYYPLPLHLQECYRSLDYQAGDLPNAE
                     KRAKEVISLPVFPELTDAEQTYVIDIIREFVGA"
     gene            3708..4769
                     /gene="wecB"
                     /locus_tag="AC16GM_09850"
     CDS             3708..4769
                     /gene="wecB"
                     /locus_tag="AC16GM_09850"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR2924010.1"
                     /translation="MKVASVVGARPQFIKLAVLSPALRRRHREVIIHTGQHYDRILSD
                     VFFRELSIPRPDYNLGIGSGEQGEQIGRGITRLEKVLEQVQPDLVLVYGDTNSTLIGA
                     VTAKRLGLQLGHVEAGLRSYRMTMPEEQNRVVADHLADLLFCPTPTAVKNLKLEGVTK
                     GVYRTGDLMLDAVKKFFPVAKSKVKILGLLGLQRKGYFLVTIHRAENTDNSKNLRRIV
                     ELVTTLPGPVIFPVHPRTRKELLRQNLWRRLRHANQVQTISPVSYLEMLVLIEAARIV
                     LTDSGGVQKEAYFLRTPCLTLRDETEWLETLEGGWNRVVGLVGIPKRSFAQSRVRPTP
                     RLSRFGGGRALKNTLAHLE"
     gene            4788..6194
                     /locus_tag="AC16GM_09855"
     CDS             4788..6194
                     /locus_tag="AC16GM_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR2924011.1"
                     /translation="MANQTLTLRRTVFPLPEQVVRNAGGLLILLTGLVATILVASRYP
                     LPFLGIVGATIAFCVAIHNFVWGVSLLIVTLYFPHPLIVQPAIRLSDVMAIIVFCAFL
                     VRRLMVGSLSWRRTPLDYPIAAMLISISLSLAVANFSYNALMGFARFLEIFAFLYAIA
                     QEVKFKDVPRLLRAYANISAAFSLVVLAELMKTGGAVRVWGLAGGYYTGLVGVAIPIA
                     FALFLYEHRGHRVWFRGAVFVTLIVSLLATQTRGFSIAVALTLIVAGLFAVSRSSLAK
                     AVVRRRLFSLVFVSVLFGAGIFLIYPSFFFGVEHRFASLTYGVVGTVQLRYFLWWLAL
                     QLFFTHPLLGAGLKQFEVAKARFFPHLQQSEMLIRVKDTYAHNQILTLLSEQGLVGLT
                     ALLWLFLTYFRLASHAFLRAAASQQATALALLGGACFVIISSTFVGEWFQPISSIQFM
                     FLLGLTTAYTRDALREDP"
     gene            6197..>6795
                     /locus_tag="AC16GM_09860"
     CDS             6197..>6795
                     /locus_tag="AC16GM_09860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2924012.1"
                     /translation="MVSEVSIIIVNFNCGDHLRVCIASLYEHLRVPFEVIVVDNHSSD
                     RSVLAVQEVFPTTRIILNRSNVGFARASNQGAAIACGDILLFLNPDARLIDDGLNALF
                     HTITNNPQIGAMGTAFILPNGTLVKSILFRIEPWLYVVDSLIVFFPLVSWWLWRRMRK
                     ETRNFTQHARVDWLIGACMVIRRSLFATLGVFDEGTFLYG"
BASE COUNT         1470 a         1809 c         1949 g         1567 t
ORIGIN      
        1 cggcgcgact tggccggtgc cgaagacagc ttgaaagcgt tccaaaagct ccacaaaacg
       61 gtgctcttag atgaggaaac caaggccgcg gtcaaaagcg ccgccgaact gaaggcgcag
      121 attaccaccg acgaaattca gctggcggtg ctcaaaaaga cgatgggatc cgatcatcca
      181 gaagtccaaa aacttacgca gaaaatcgag gagacgcgcc ggcaattggc caaaatcgag
      241 ttcggcgggg cgaggagcga ccccaaagga tacctctcca tcccatttga aaaattcccc
      301 gacgttagcc tccagctggc tcagctcatg cgcgatctag cggtgcagga aaaagtgctg
      361 gaacttttga cccagcagga cgaacaggcc aaaattcaag aaaagaagga tacacccacg
      421 gtgatggtgc tcgatccggc ggtgccggga agcaaagtaa ggccgcggcg ttcccttttg
      481 gtcgtcttag tcggcggact ctccttggtg gcaagcattt tctacattct ggcggcggag
      541 tactttcgca aactccgctt tgtgcggccg gaagaatact cgcgcgtggc ggagatcaaa
      601 actgccgtgg tccacgacct gcctctggtc gggcgtcttt tcaaccggac caaggcgccg
      661 ttcaccgagg gcgaccggtg aaaccggtca atgtggcggt gatcggcatc ggcgcctggg
      721 ggaagaatct cttgcgcaat ttcgccagtc tcaaagggtg cgcggttacc gtcggctgcg
      781 acgccgacgc gaagaacaaa gccaccgtta ccaacctggc cccgacagcc accttcacca
      841 ccgatttccg gcaggcgctg gatgccgatt cggtggaagc ggtggtcatc gccacgccgc
      901 ccgccagcca ctttgccatc gcccgggcag ctctggaagc gggcaaagac gtgttcgtgg
      961 aaaaaccgct caccctgaag gtaggcgacg gtgaggcctt agtccgttta gcggaagagc
     1021 agcagcggat cctgatggtg gggcacatca tggaatatca cccggcggtc ctcaaactga
     1081 agggttacgt gacctccggc gaactgggga agatttacta cctctatgcc agccgggtaa
     1141 acctgggcaa agtgcgggac accgaaaatg cgctctggag ttttgccccg catgacattt
     1201 cgctcgttct cttccttctg gagcaaaaac cagtgaaggt aacggccaac ggtgccgcct
     1261 acttgaagcc gggcattccc gatgtggctt tcctctcctt gctgtttgcg gacggggtcc
     1321 tggcccacat ccacgtttcc tggctggacc cgcataaaga ccgtaagctc accgtggtcg
     1381 gttcccgcaa aatggcggtc tttaacgatg ccgaagcaag cgagaaaatc tggatttacg
     1441 ataaaggtgt tgacacgaag ttagactaca acacctacgc ggagttcttg agcctgcgca
     1501 ccggggatat caccatacca aaggttgacg gcggcgaacc tttggccgtg gaatgccagc
     1561 acttcctgga ctgtgtggcc aaacgcaccc agccccgcag cgacggccag gatggtctga
     1621 cggttctgcg cgtcctggat gcggcgcagc ggtcgctgga tcagggggga actccggtgg
     1681 aggtgcggta cgcttaagga gtaactcatg cagccagtca ttgcaacgag tgcgaaggtc
     1741 ggaaaaaaaa ttcggtgggg acaattctgc gttgttggtg aaggagtcac catcggcgat
     1801 gggtgtctga tcggcaacca cgtgacgatc cacaacggga cggtcatcgg caataacgtg
     1861 cgcatcgatg acaatacggt catcggcaag cagccgatgc gggcggccaa ttccgcggtt
     1921 accaaggagc aggaactgcc gccggcggcg atcggcgacg actgcattat cggcacctcg
     1981 gtcgtcattt acgccgggtg caaactgggt cggaaaatac tggtggccga cctttcgacc
     2041 gtccgggaga acgtcacggt cggagacttc accatcattg gccgcggcgt ggccatcgag
     2101 aacttctgca ccatcggctc ttactgtaaa cttgaaacca acgtctacat caccgcctac
     2161 tcacagattg gcgaccgggt gttcgtagcc ccctgcgtgg ccacgtccaa tgacaacttt
     2221 gtggggcgga caaaggaacg ctttaagcac tttaagggag tcacggtcaa gaccggcggg
     2281 cggatcggga ccaacgccac aatcctcccc ggaaaggtca ttgctgaaga cgcgctcgtc
     2341 gccgccgggt cggtagtgac caaagacgcg ccggcgcgga aaatcgtcat gggcgttccg
     2401 gcgcgggtgc tacgggacgt gccgaaagag caattgctgg aaaaccagta gaaaagtcga
     2461 attgaagact gaggattgag aactgaggga aaaacactga atgatcttga ttgaagtgct
     2521 cgcggttttg cgaaggtcgc ccgcgatcac gacggagata ccatgactgc gatcagcgaa
     2581 acgacaaaaa aagcggcacc cgttgcagtg aaagtgccgc ttttggattt gaaacggcag
     2641 taccaagcga tcaaatctga aatcgatgcg gcggttatga aggtcttagc cgagacccag
     2701 ttcattttag ggccggaagt caaagaattt gaacagacaa tcgcccagta cttaggtgtc
     2761 aaacacgcga ttggcgtcgc ttccggtacc gatgcgctcc tgctctcgct gcgcgtctgc
     2821 ggggtgggac cgggggacga agtagttacc tccacgttct cgtttttcgc ctccgcagga
     2881 gtcatttccc gcctgggagc cacaccggtc ttcgccgaca ttgacccgaa aacttacaac
     2941 cttgatccga agtcaatcgc cgccaaaatt accaaaaaga cgaaggtcat tatgccggtg
     3001 cacctgtacg ggcagtgtgc ggaaatggat ccaattctgg aattggaccg ctcgcacaaa
     3061 ctcgacgtcg tcgaagacgc tgcccaggcc atcggtgcca catacagggg acgcaaggcg
     3121 ggaaccatgg gccggttcgg ctgcttctcc tttttcccca gtaagaattt gggcgggatg
     3181 ggcgacggcg ggctcatcgt gacgaattct gacgacgatg ctgaactgtt gcgccggctg
     3241 cgggtgcacg gctccaagcc gaagtactac cacagtgtcg tgggctacaa cagtcggctg
     3301 gacaccattc aggccgccgt gcttcaggtc aaattaaagt acctcgatgc ctggaccgcc
     3361 gcgcggcggg agaaagtcaa attctataac cggggtttgg cgggcagtcc ggtggagacg
     3421 cctttcgaac cggaatacaa ccaccacatc taccaccagt acacactcgc tctgcgtgac
     3481 cgcgatggac tgaaggcacg cttcgccgag cgggggattg gctgcgacac ctactatccc
     3541 ctgccgctgc acctgcagga gtgctaccgc tccctggact accaggccgg tgatttaccg
     3601 aacgccgaaa agcgggcgaa agaagtgatt tccctgccgg tctttccgga gttgactgac
     3661 gccgagcaaa cctatgtgat cgacatcatc cgggagtttg tgggcgcgtg aaggtggcgt
     3721 cggtggtagg cgcccggccg cagttcatca aactggcggt tctgtcgccg gcattgcgga
     3781 ggcgacaccg tgaagtgatt atccataccg gccagcacta tgaccggatc ttatccgatg
     3841 tctttttccg cgaactttcc attccccgcc cggactacaa cctcggaatc ggctccggcg
     3901 aacaaggtga gcagattggg cgggggatca cccggctgga gaaagtctta gaacaggtgc
     3961 aacccgattt ggttttagtc tacggcgata cgaattcgac tttgatcggt gcggtgacgg
     4021 cgaaaaggct cggcctgcag ctggggcacg tagaagcggg actccgcagt taccggatga
     4081 cgatgcccga ggagcaaaac cgggtggtgg cggatcatct ggctgacttg ctcttttgcc
     4141 ccacgcctac ggcagtcaaa aatctcaagc tggagggggt caccaaaggt gtctaccgga
     4201 ccggtgacct gatgctggac gcggttaaga agttctttcc ggtggcgaag tcgaaagtta
     4261 agattttagg tctactcgga ctgcagcgca agggctattt tctggtgact atccaccggg
     4321 cggagaatac ggataattcg aagaatttac gccgtattgt ggagctcgta accacgctcc
     4381 ccgggccagt cattttcccc gttcacccgc gcacgcggaa agagttgctc cgccagaatc
     4441 tgtggagacg gctacgtcac gcaaatcaag tacagactat ctcgccggtg agttacctgg
     4501 agatgctggt cttaatcgaa gcggcacgaa ttgttttgac cgactccggc ggtgtgcaga
     4561 aggaggccta ctttctgcgc acgccctgcc ttactttgcg ggacgaaact gaatggctgg
     4621 aaacgctgga aggcggctgg aacagggtgg taggattagt cggcattccg aagaggtctt
     4681 tcgctcaatc gcgcgtgcgt ccaacgccac ggctttctcg tttcggcggc ggacgggcgc
     4741 tcaagaatac actggcgcat ttggagtaat catcggagtg gaacaccgtg gctaatcaga
     4801 cactgaccct gcggcggacg gtttttccct taccggaaca agtggtccgt aatgccggcg
     4861 ggcttttaat ccttttgacc ggcttggtgg ctaccatttt ggtggccagt cgctacccac
     4921 tgccttttct gggtatagtg ggagcgacca ttgcgttctg cgttgcgatt cacaatttcg
     4981 tatggggggt atctctactt atcgtaacgc tctactttcc tcacccgtta attgtccaac
     5041 ctgctattcg attgagtgac gtgatggcca tcatagtttt ctgtgccttc cttgttcgac
     5101 ggcttatggt cggaagtctt tcttggcgac gtacaccact tgattatcct attgctgcca
     5161 tgcttataag tatcagcctc tctcttgctg tcgcgaattt ttcctacaat gcgctgatgg
     5221 gtttcgcccg ctttttggaa atattcgcct ttttgtacgc aattgctcag gaagtgaaat
     5281 ttaaagacgt tcctcgtctg ttgcgggcgt acgcaaacat ttctgctgct ttttcacttg
     5341 tagtgctagc agaacttatg aaaacgggag gggctgttcg ggtatgggga ttggccggcg
     5401 ggtactacac cggcctagtt ggggtagcaa ttccaattgc cttcgctctt ttcttatacg
     5461 aacaccgagg acatcgggtc tggtttcgag gggccgtgtt tgtgacctta atagtttctt
     5521 tattagctac gcaaactcgg ggatttagca tcgctgttgc tctgacgctt atcgtagctg
     5581 gtttatttgc tgtttcgcgc tcttctttgg cgaaggcagt tgttcgccgt cgactgtttt
     5641 cgctcgtttt cgtttcggtc ctttttggcg caggtatttt tttaatttac cccagtttct
     5701 tcttcggtgt ggaacaccga ttcgcctcgc tgacgtacgg ggttgtagga actgtgcagt
     5761 tgcgatactt cctttggtgg cttgccttgc aactgttttt cacccatcct cttctggggg
     5821 cgggccttaa gcaatttgaa gtagccaagg ccagattttt tccacacctg cagcagtcag
     5881 agatgcttat tcgtgttaag gatacatacg cgcataacca aatccttacg ttgttgagtg
     5941 agcaaggatt ggtagggttg actgcactcc tgtggttatt tctcacgtat ttccgtttgg
     6001 ccagccatgc gtttctacga gcggcagcgt cgcagcaggc aactgctctc gctctcttag
     6061 gaggcgcttg ctttgtaatt atatcttcaa cgtttgttgg cgaatggttt caacctatta
     6121 gtagtattca gttcatgttc cttctcgggt tgacaactgc atatacgcgg gacgcgctac
     6181 gggaggatcc ttaactatgg tatctgaagt ctcaatcata attgttaact tcaattgcgg
     6241 cgaccatcta cgggtctgca ttgcctccct ctacgagcac ctgcgggtgc cgttcgaagt
     6301 gattgtggta gataatcact cttcagatcg ctcggtactt gctgtccagg aggtatttcc
     6361 cactacccgg atcattttga atagaagtaa cgtgggcttt gcacgggcga gcaatcaggg
     6421 agcggccata gcgtgcggtg atatccttct ctttctgaat ccggacgcac gcttaatcga
     6481 tgacgggttg aatgcgctct tccacacaat tacaaacaat ccccagatag gggccatggg
     6541 gaccgccttc attctgccca acggcacgct ggttaagtcg atcctcttca gaatagaacc
     6601 gtggctctat gtggtggatt ccctcatcgt attcttcccc ctagtgtctt ggtggctgtg
     6661 gcggagaatg cgtaaggaga ccaggaactt tacccagcat gctcgtgtgg actggctcat
     6721 cggagcctgt atggtgatcc ggcggtcact gtttgctaca ctaggagtat ttgacgaggg
     6781 gactttcctc tacgg
//
LOCUS       DBNGZD010000374         1544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_682321, whole genome shotgun sequence.
ACCESSION   DBNGZD010000374 DBNGZD010000000
VERSION     DBNGZD010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_682321"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="AC16GM_09865"
     CDS             complement(<1..1015)
                     /locus_tag="AC16GM_09865"
                     /inference="COORDINATES: protein motif:HMM:NF017505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HIR2924013.1"
                     /translation="MTRQIVKFGFGLIFLAGTVVVSVQCAKKAAQVSAGDKYLFVWTG
                     DQARKAPDFLAVINFDQNAPDYGKVITTVPLPNPGNTWNEPHHVGLSRDGKVLACGGL
                     LSILKGQKEVFFFDVSDPAQPKFLSAADPPQSGVADEFYALDNGGYLVTMMGGAEGHH
                     PGRVAEFDKDLNLVAEYPQDPPQDGFNPHGVSVRPEVNLMVTSDFICPSTTLHAVPGD
                     PGFRGSVRVWDFQKRTILRTITLPNNAAGTIDVRLIPQDPQKRGYTAGMTDDKLYLLD
                     TQAGTAKPVFDFATIAKGGWPQLMRMTKDGKRLFISMNMAGKVVMFDTSDPEKPEVIK
                     VLEL"
     gene            1188..>1544
                     /locus_tag="AC16GM_09870"
     CDS             1188..>1544
                     /locus_tag="AC16GM_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR2924014.1"
                     /translation="MILPPSLSFAQTPTPLEKLRFDHPAQVTLYLKRDDLTGSLLSGN
                     KVRKLEFIFAEILKKKADAVITCGGMQSNHARATSILAAKFGLKTVLFLHPIPENSQV
                     PDGNLFLDRLAGAEVRF"
BASE COUNT          332 a          457 c          380 g          375 t
ORIGIN      
        1 ccaactccag cacttttatc acctccggct tttccgggtc agaggtgtca aacatcacca
       61 ctttgcccgc catgttcatt gagatgaata accgcttccc gtctttggtc atccgcatca
      121 gttgcggcca gccgcctttg gcaatggttg cgaaatcaaa aaccggtttg gccgttccgg
      181 cttgcgtatc caacaggtag agtttatcgt cggtcattcc cgcagtgtag cctcgcttct
      241 gtgggtcttg tggaatcaac cgcacgtcaa tcgttccggc tgcattgttg ggcagggtaa
      301 tcgtgcgtag aatagtccgc ttctggaaat cccagacccg cacgctgccc cggaatcccg
      361 ggtcgccggg aaccgcgtgt aacgtggtgg aagggcagat gaagtcactg gtgaccatga
      421 gattgacctc cgggcggacc gataccccgt gcggattaaa accgtcctgc ggggggtctt
      481 ggggatattc ggccaccaag ttcaaatctt tatcaaactc ggcgacacga ccgggatgat
      541 gcccctctgc cccgcccatc atcgtgacca aatacccacc gttgtccagc gcatagaact
      601 cgtctgcaac acccgattgg ggcggatcag ccgccgagag aaatttcggc tgagccggat
      661 cagatacatc gaagaaaaaa acttctttct gccctttcaa aatgctcaaa aggccgccgc
      721 aggcgagaac cttgccgtca cgggaaagcc ctacatggtg cggttcgttc caggtatttc
      781 cgggattggg caggggaaca gtagtgatga ccttgccgta gtccggtgcg ttctggtcga
      841 agttgatcac cgctaaaaag tcgggggctt tccgcgcttg gtctcccgtc cagacgaaca
      901 agtatttgtc tcccgcagat acttgggcgg ctttcttcgc gcattgaacc gatacaacca
      961 ccgttcccgc cagaaaaatt aggccgaatc cgaactttac aatttgcctc gtcatgaccc
     1021 accctccttt ggcccatcca gttacagaaa tttcactcct ggttctttcc gatttttcgg
     1081 gaagaaccat cccattccct ttgaaatatt taagtacaaa ccattgcgtt tgtcaagact
     1141 ctttttgtct cctttgacag acgggccgat ctttatatcc tatccttatg attttgccac
     1201 cctctctctc ctttgcccaa actcccaccc cgctggaaaa attacggttc gatcatccgg
     1261 cgcaggtaac gctctacctc aagcgcgatg acctgaccgg ctccctcctt tccggcaata
     1321 aggtgcgtaa gttggaattc attttcgcgg agattctgaa aaagaaggcc gacgcggtga
     1381 tcacctgcgg cggaatgcag tccaatcacg cccgggccac gtcgattctg gccgctaaat
     1441 tcggcctgaa gacggtgctt ttcctccatc ccatcccgga aaactcgcaa gtgcctgacg
     1501 gtaacctgtt tctcgaccgg ctggcgggag ccgaagtccg cttc
//
LOCUS       DBNGZD010000375         3985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_268219, whole genome shotgun sequence.
ACCESSION   DBNGZD010000375 DBNGZD010000000
VERSION     DBNGZD010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_268219"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..992)
                     /locus_tag="AC16GM_09875"
     CDS             complement(204..992)
                     /locus_tag="AC16GM_09875"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR2924015.1"
                     /translation="MKQGVKLFVLLALSTLFISVATIATAGDTKNGEAKKTYWKITGQ
                     LEEACSCNPACPCWFDSKPTRMTCGGGQVLFIEEGTYGDVPLKGLAVANMVESPPGQS
                     MMESFGKWKFSYLYIDEKASPEQRQALEELGKTVLSSHASSPNVKIRYVPIVRAIEGK
                     EHKITIGDYGSFHGHLIEGGLGGTPKIVNPPGADPIHQEYQQGQTTKLTYSDAEQNWN
                     YEKSNYMFTTFTVDSDQYEKYAAGLAQKMKAMKKQEGTEKGNRK"
     gene            complement(1201..1833)
                     /gene="grpE"
                     /locus_tag="AC16GM_09880"
     CDS             complement(1201..1833)
                     /gene="grpE"
                     /locus_tag="AC16GM_09880"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR2924016.1"
                     /translation="MSKKKEKGEEKVTVRNTVPLPSAQEAGAPPRRVESTTEAVPTLE
                     ETLRNQLDESETRFKELEDRYLRLAAEFDNFRKRTNRDLATIIKNANENLILQQIDIV
                     DNFHRALDAAKTSADFDSFHKGVELIYTQMFDILNREGVQIIEPAGQVFDPHFHEAIM
                     YVESDQPEGKVIEVVSTGYLLNDKVLRPARVVVSKGKTRASPENREEEKS"
     gene            complement(1905..2939)
                     /gene="hrcA"
                     /locus_tag="AC16GM_09885"
     CDS             complement(1905..2939)
                     /gene="hrcA"
                     /locus_tag="AC16GM_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR2924017.1"
                     /translation="MTKPFENLNEREREILHALIDHYIRTADPVGSRILATRYPFGLS
                     PATIRNTMQDLESRGLVEQPHMSAGRVPTDAGYRLYVDNLLRPEQLTEEEKERIRNEV
                     VLDQVAVDRILEQTSRVLAGVSHQLGVTIAPSFEQGVLTRIDLVPVTERKLLVIIAVK
                     SGLAKSVLLEVDVAIDERLIGETARLLNERLAGLTLGEIKRTIRERLSAPGEGDPKLL
                     RLFLNASDQLLSAEEAEHLHIEGTANVVNQPEFKDHNRLTTLFSLLEERKSIVEMLNT
                     QGIKEGIVITIGSENPRAEGQNLSVVTSTYQAGKVKGTIGVIGPTRMPYSKLVSVVDY
                     TAKVLSEIFS"
     gene            complement(3008..>3985)
                     /locus_tag="AC16GM_09890"
     CDS             complement(3008..>3985)
                     /locus_tag="AC16GM_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HIR2924018.1"
                     /translation="GVRTTVYLLLAIVITNYANTVSEFAGIAASLEIFNISRHLSVPV
                     GAILVWLLVVKGNYKTVEKVFLVACLVYFTYIVSALMARPDWSEVLRAVKATDISFDS
                     KSLAMVIGLVGTTIAPWMQFYLQSAIVEKKIHVNHLKHSRWDVISGSVIVNVVAFFII
                     VACGATLFVKGVQIETAKEAALALAPLAGKYASYLFAFGLLNASLFSASILPLSTAYM
                     VCEGMGWQDGVDKKFSEAPLFYLLYTLLILGGAVTVLLPGIPLIKIMYFSQVLNGIVL
                     PLILVFMLLLVNDRRLMGKYVNGPVFNAIAIVTAGTLIVLSILLVSFVR"
BASE COUNT          852 a         1095 c         1125 g          913 t
ORIGIN      
        1 gaaggtaaat ccgaaaaagc cagcgaaaat gaaagagtaa aggcaaacgc cttgcgcggg
       61 acgtttgcct tctcagtgac ctttcttggt gccccggagg tgggcacttg ctatggcaac
      121 accctgcctc ttgcaaccgt cgaactttga gggcgcaaca gaacgtgaag acagtgactg
      181 aacgtcagcc cttacgtctg ccgctatttc cggttcccct tttctgtccc ttcttgcttt
      241 ttcatagctt tcatcttctg cgcaagtcca gcggcatact tctcatactg atcgctgtcc
      301 acggtgaacg tcgtgaacat gtagttggat ttctcatagt tccagttctg ctccgcatcg
      361 gagtaagtaa gtttggttgt ctgtccctgc tgatactcct gatgaatcgg atcagcgccc
      421 ggtgggttga cgattttggg cgtgccgccg agccctccct cgatcaggtg cccgtggaaa
      481 ctcccgtagt caccaatcgt gattttatgc tccttgcctt cgatcgcccg aacaatcgga
      541 acgtagcgga ttttaacgtt tggcgacgaa gcgtgagatg aaagcaccgt cttcccgagc
      601 tcttcgagcg cctggcgctg ttccgggctg gccttctcat cgatgtagag gtaggagaac
      661 ttccacttgc caaaggactc catcatcgac tggccgggag ggctttccac catgttggca
      721 accgccaggc ctttgagcgg cacgtcccca taggtgcctt cttcaatgaa gagaacctgg
      781 ccaccaccac aggtcattcg tgttggtttg gaatcgaacc agcacgggca ggctggatta
      841 caggaacaag cctcttccag ttgccccgtg atcttccaat acgttttctt cgcttccccg
      901 tttttcgtgt caccggcagt cgcaatcgta gcgacgctga tgaagagagt ggataaggcg
      961 aggagcacga agagctttac accctgtttc atagtacctc ctttcgctta gcggcctcaa
     1021 ttatagaagc aatgagtcct gtttgtcaag aatttatttc tgtactcgct aaattattct
     1081 gccggccaaa cccaggcaat ctgttctgcg aagaaaatag atgacatccc tttggatacc
     1141 agtaggttca agaggctata taaagttttg ctttaatcga aatgccgcgg atgcgggcct
     1201 tcaagatttt tcttcttccc ggttctcagg agatgctcgc gtcttgcctt tggacaccac
     1261 gacccgcgcc gggcggagga ctttgtcgtt aagcagataa ccggtggaga cgacctcgat
     1321 aactttacct tccggctggt cggattcgac gtacattatc gcctcgtgaa agtgcgggtc
     1381 gaaaacctga cccgctggtt cgatgatctg cactccttcc cggttgagaa tgtcgaacat
     1441 ctgggtgtag atcagttcaa cgcctttgtg gaagctgtca aaatccgcgg aggttttggc
     1501 cgcgtccagc gcccgatgaa agttgtcgac gatgtcgatc tgttgcagaa taagattttc
     1561 attggcgttt ttgatgatcg tggcgaggtc gcggttggtg cgcttgcgga agttatcaaa
     1621 ctcggccgcc agccgcaggt agcggtcttc caactccttg aagcgcgtct cgctttcgtc
     1681 taactgattg cggagcgttt cttccagcgt gggaaccgcc tcggtcgtag actcgactct
     1741 tcgaggcggc gctcccgctt cctgagcaga cggtagcggg actgtgtttc gaaccgtcac
     1801 tttctcttct cctttttctt ttttcttact catcacttac cctcgacaca caaagaaggg
     1861 cggcgcactt caccgcccct gctgacgctc ggcgattccc ggttctacga gaaaatttcg
     1921 ctcaatactt tggcggtgta atcgaccacc gagaccagtt tggaataagg catccgggtg
     1981 gggccgatga cgccgatcgt gccttttacc ttccccgcct gataggtgga ggtgacgacg
     2041 ctcaaattct ggccttcggc ccggggattc tcgctgccta tggtaatgac gatgccttct
     2101 ttgattccct gggtgttcag catctcgacg atcgacttcc gctcttccaa aagcgagaag
     2161 agcgtggtca accggttgtg atctttgaac tccggctgat tcaccacgtt ggccgtacct
     2221 tcaatgtgga ggtgctcggc ctcctcggcg gaaaggagct ggtcggaagc gttcaggaac
     2281 aggcgcagca gtttcgggtc accttccccg ggagccgaaa ggcgctcccg aatggtgcgc
     2341 ttgatttcgc ccagagtgag gccggccagg cgctcgttca aaagccgcgc cgtttcccct
     2401 atcagccgtt cgtctatggc cacgtcaact tccaaaagga cgctcttggc caggccggat
     2461 ttaaccgcaa tgatgaccaa aagtttccgc tcggtcaccg gcaccagatc gatgcgggtc
     2521 aagaccccct gctcaaaact gggggcgatg gtcactccca gctggtgcga cacgccggcc
     2581 agaacccgcg aggtctgctc cagaatccgg tccaccgcca cctggtcgag caccacctcg
     2641 ttgcggatcc gctctttttc ctcttccgtc aactgttccg ggcggagcag gttatccacg
     2701 tagaggcggt agccggcgtc ggtgggcaca cgccctgcag acatatgggg ctgttcgacc
     2761 aacccgcgcg actccaaatc ctgcatcgtg ttgcggatgg tggccggaga caagccgaag
     2821 ggatagcggg tggccagaat ccgggagccg accgggtcgg cggtgcggat gtagtggtcg
     2881 attaaggcgt ggagaatctc ccgctcgcgt tcattcaagt tttcgaaggg cttagtcata
     2941 ggagtaatta taatttatag aaaaatttgc tcctgtcaag aattaggacg gactaacgcc
     3001 gtgggcttca gcgtacgaag gagactaaca ggatcgagag cacaatcagc gtaccggcgg
     3061 ttacgatggc aatggcattg aagaccgggc cgttgacgta cttgcccatg agccggcggt
     3121 cgttcaccag gagcagcatg aagactaaaa tcagcggcag gacgatgccg ttgagcacct
     3181 gggagaaata catgatttta atgagcggta tccccggcag aaggacggtg acggcgccgc
     3241 ctaaaatcag aagcgtgtag aggaggtaaa agaggggcgc ctcggagaac ttcttgtcca
     3301 cgccgtcctg ccagcccatc ccctcgcaga ccatgtaggc ggtggagagg ggcagaatcg
     3361 aggccgagaa aagcgaagcg ttgagcaggc cgaaggcaaa gaggtaggaa gcgtattttc
     3421 ccgccagagg cgccagggcc agcgccgctt ccttggccgt ttcgatctga actcctttga
     3481 cgaacagggt cgccccgcag gccacgatga tgaagaaggc caccacgttg acgataaccg
     3541 agccggaaat cacgtcccag cgggagtgct tgaggtggtt gacatgaatt ttcttctcca
     3601 cgatggccga ctggaggtag aactgcatcc agggggcgat ggtggtgccg accagaccga
     3661 tgaccatggc cagggacttt gagtcgaaag aaatgtcggt ggctttcaca gcccggagca
     3721 cctccgacca gtccggcctc gccataagcg cagagacgat gtaggtgaag tagaccaggc
     3781 aggcaaccag aaagaccttc tccacggtct tgtaatttcc cttcaccacc agaagccaga
     3841 cgagaatagc tcccaccggc accgagagat gccggctgat gttgaagatt tccagactgg
     3901 cggcgatacc ggcgaattcg gagaccgtat tggcgtagtt ggtgatgacg atggccaaga
     3961 gcaggtagac cgttgtgcgc acgcc
//
LOCUS       DBNGZD010000376         8163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1096104, whole genome shotgun sequence.
ACCESSION   DBNGZD010000376 DBNGZD010000000
VERSION     DBNGZD010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1096104"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1255
                     /locus_tag="AC16GM_09895"
     CDS             128..1255
                     /locus_tag="AC16GM_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924019.1"
                     /translation="MGHAYTPGLKVTDRMLVTKRRILPLKGDVLVKVGDRVEPDTVVA
                     RTLLPGPVEPVNVANILGVPPEDVPQTMLKKVGDPVKTSEVIARSKSFFGLFSSECKS
                     KIEGRVESISSVTGQVLLRGHPVPVEVKAYLKGEVVEIFEKEGCAVTTRGAFVQGIFG
                     IGPETHGYIKIATRDNRQVLSEDLIDESMKGQVVVGGSLVTAGALNKAIRAGAAGIVV
                     GGFDDKDLRDFLGYDLGVAITGSEEKGITLVVTEGFGQINMAERTFTLLKQNEGKLAC
                     FNGATQIRAGVIRPEVVIPLVGDIRQDEAAEKFAEEGLAEGSPVRVIRQPYFGHLGKV
                     TTLPPELQKLESESHARILQVEFADGTRAIVPRANVEMLEG"
     gene            1293..3221
                     /locus_tag="AC16GM_09900"
     CDS             1293..3221
                     /locus_tag="AC16GM_09900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR2924020.1"
                     /translation="MKKIRKLWFAPLSILLFMAGLSTANAYEFTQYQNIKHANSPSFS
                     PDGKTLAFMTNVTGVYQGWTIPVTGGWPEQATFYEDGVDFFVYSPMEDKIAFGKDTGG
                     DERTQLYLCSPRGENIVQLTNNPKVIYSFGGWSHDGKKIAYAANERDQAYFDVYVRDL
                     AIDQAKLVYQSDANNSVAAWSPDDRYLVVARANTNLDFDLFLVEAATGTSTHLTPHTP
                     PAVYESIEWTNDGKGFYLASDQDRDFVNLAYYDVAEKKLTFLDDGKSDVDGLKLSDDN
                     RYLAIVRNIDGYGRLFLREHPLRVGTITQAKTSEVHLPDGIPSGLTFSKDGKMMAFAF
                     VGPTQNGNIWTYEMAGEKLTEVTRSTLAGIPPSSFVAPTLVKFKSFDGLELSAFIYLP
                     TGAKKDGSAPCIVMVHGGPESQERPYFATTYQYYLSRGYAILAPNIRGSTGYGKTYTH
                     LDDIEKRGQAIQDVKACYNFLKNSGYVDPAKVAVMGASYGGYMTLAAVTFLPELWAAG
                     VDIVGIANFETFLKNTGAWRRKLREAEYGYLDKDIEVLRKYSPIHAVDKIKCPLFVIQ
                     GANDPRVPQSEAEQIVDQVKKQGGAVEYMLFPDEGHGLAKLANRIKAYPAVAEFLDKH
                     VKNRGVVQAGDSSGEKEK"
     gene            3316..3918
                     /locus_tag="AC16GM_09905"
     CDS             3316..3918
                     /locus_tag="AC16GM_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HIR2924021.1"
                     /translation="MRKSLLVLAVGLFAVTSIVAQQEQSKSNKPAEPSMEMMMPKPGP
                     ELAKLDYFLGTWTGEETMNSPGMPPGSKNKSKTVTKRGIGGMFLVGDYTSSMGEGKNA
                     MKFAGHSLWTYDPQKKGYRHWWFDSFGDGQETPGNWVDDKTYVGETSYEMMGQQVKQR
                     QTYAILSPTQFTMKIEMDNGQGWMTFLEGTYTKQTQKPGK"
     gene            3947..4555
                     /locus_tag="AC16GM_09910"
     CDS             3947..4555
                     /locus_tag="AC16GM_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HIR2924022.1"
                     /translation="MRKILCVLALCSIALTVAAAQQQEKSKSGQPEGQSMGMMMPKPG
                     PELAKLDYFLGTWTGEETLNYPGMPAPAKSKAKYTAKRGIGGMFLMGDYNATMSDGKT
                     EQKMEAHTLWTYDPQQKNYRLWGFDSFGTAMEMRGNWTDDKTFVAETSTEMGGMPVKL
                     RDTYTLVSPTTCTLKSEMDMGQGWQTGMEGTYTKQTETKKPK"
     gene            4839..6017
                     /locus_tag="AC16GM_09915"
     CDS             4839..6017
                     /locus_tag="AC16GM_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR2924023.1"
                     /translation="MRLTAPVRIFFLAVLLSLAWVLPAWAIAPPLDVQAKDTPNDAGK
                     SITVTWKKSPDDRPGVAGVAEYRILRGTSLDGPSTFVGTATGGTELFTDNTAQNNINY
                     YYKIVAVAGDKSEAESLPSAPTHAYQQWFNAKRVNLLIGVLLLSFAIIYFIEQAKKGK
                     PLFIRKIAGLEAVDEAIGRATEMGKKVLYIPGIQDLDNVQTVAGVTILGRVAKVAAEY
                     ETHLEVPTSRALVMVACREAVQEGYSLAGRPDAYNQDRIYYLTDEQFGYAAGVDGIIV
                     REKPAAIFYQGCFFAESLILSETGNSIGAIQIAGTAQPTQLPFFFAACDYTLIGEELF
                     AASAYLSREPRQLGSLKGQDIGKALIMLAVLAGAIWQTLAVFFPETFHANAASLFLTE
                     "
     gene            6024..6677
                     /locus_tag="AC16GM_09920"
     CDS             6024..6677
                     /locus_tag="AC16GM_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924024.1"
                     /translation="MRREIPLILTAIVGLTLVIQFFIPHEPFNKMQTWFADWVAIIQA
                     FAIWLGALNLLVVSAEKVSKKQPGWFYSVVIIASFFLIVIPGLFFSGGRHFQEAGTSF
                     DWLYAHMFTPLSATMFALLAFFVASASYRAFRARNKEATLLLLAGIFVMLGRVPIGDV
                     LTNFLPEKIRLSSVADWIMAYPQLAGQRAIMIGIALGIVSTSLRIILGIDRTIVGGK"
     gene            6677..7549
                     /locus_tag="AC16GM_09925"
     CDS             6677..7549
                     /locus_tag="AC16GM_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924025.1"
                     /translation="MAEVPNVPETQRGFFDRLVAIDRRVIFLFVGLALLVPLFMNVSQ
                     KMKISPEVQSLFDAMQALPPGSKVLLACDYDPPSSPELQPMAVTAVKYCFLYRLKMIV
                     IGLWPQGPQQADLAIRAALKDPEVAKVNPQYGIDYCNLGFQAGNEVVIQKMGSDIPGT
                     FPKDWRGTDVKTLPIMTGIKNFSNIDFIFNLSAGYPGTREWVQLAADRFHAKLVSAAT
                     AVQTPEYTPYLGRQLLGILGGMKGGAEFEALTTYRGKATKFMLAQSLSHLIVVAFIII
                     GNAAYFASRKKKGA"
     gene            7553..>8163
                     /locus_tag="AC16GM_09930"
     CDS             7553..>8163
                     /locus_tag="AC16GM_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924026.1"
                     /translation="MEGFTFGTTLAAILTIGILSFLYKDNPLYKLCESIFIGISAGYW
                     FVSLYFQNMLPKMFDNLGITKALGSKEAGDGALQVLLHQHHWDERWIFLVAGVLGIMM
                     LLRLFPKIGWISRWPLAFVVGYTSGLYFIAYLQSNVFAQVQKSVVPLATLAQSNGAIA
                     WGPTIGTLVVVFGTLAGLVYFFFSKEHKGFFGGTARLGIWFLMV"
BASE COUNT         1768 a         2308 c         2430 g         1657 t
ORIGIN      
        1 ctacccgcgg gaggcgctgg agcggttaga atcacgccga cctgaacgca aagtgatgat
       61 tgaaaaaccg gaacttgtag gcgtcaagta attgggcggc cattaccacg taaaaacaca
      121 gagttaaatg ggtcacgcct acactcccgg cttaaaagtc accgaccgga tgctggtcac
      181 caaaaggcgc attctgccgc tcaaggggga cgtgctggtc aaagtgggcg atagagttga
      241 gccggatacc gtcgtggcgc ggaccctttt gcccgggccg gtggagccgg tgaacgtggc
      301 caacatcctg ggcgtcccgc cggaagacgt gccccagacg atgctcaaaa aagtggggga
      361 tccggtcaaa accagtgaag taattgctcg ttcgaagtca ttcttcggcc tcttctcatc
      421 tgaatgcaaa tcaaaaatcg aggggagggt cgagagcatc tcctcggtca ccggccaggt
      481 gcttttgcgc ggacaccccg tgccggtgga ggtgaaggcc tacctcaagg gcgaagtggt
      541 ggaaattttc gagaaagaag ggtgcgcggt gaccacccgg ggcgcgttcg tccaggggat
      601 tttcggcatc gggccggaga cgcacggcta tatcaaaatc gccacccgcg ataatcgtca
      661 agtcctttcc gaagatttga tcgacgaatc gatgaaaggc caggtggtcg tcggcggatc
      721 gctcgtcacc gccggcgcgc tcaataaagc cattcgggcc ggcgcggcgg ggatcgtcgt
      781 gggcgggttc gacgacaaag acctgcgcga ctttttgggt tacgatctgg gcgtcgccat
      841 caccggcagc gaagagaaag gaattacgct cgtggtcacc gaagggtttg gtcaaatcaa
      901 tatggccgag agaaccttta ctttgctcaa acaaaatgaa gggaagttag cctgcttcaa
      961 cggcgccacc cagattcggg ccggggtaat ccgtccggag gtcgtgattc ccctcgtggg
     1021 cgacatccgg caggacgagg cagccgaaaa atttgccgaa gagggcttag ccgaggggtc
     1081 gccggttcgg gtaatccgtc agccctactt cggccacctg gggaaggtca cgaccctgcc
     1141 gccggagctg cagaagttgg agtcggaatc gcacgcccgt atattgcagg tggagtttgc
     1201 cgacggcaca cgcgccattg taccgcgggc caacgtggaa atgttggaag gatagaagcc
     1261 ggaacacaga acacagaaca cggggaggaa caatgaagaa aatccggaaa ttatggttcg
     1321 cgccgctctc gatcctgttg tttatggcgg gtctctcgac ggccaatgcg tacgagttca
     1381 cccagtacca gaacatcaag cacgccaatt cgccgtcctt ttctccggac ggcaaaacgc
     1441 tcgcctttat gaccaacgtc accggcgttt accaggggtg gaccattccc gtgaccggcg
     1501 gctggccgga acaggcgacc ttctacgagg atggggtcga tttctttgtc tactccccga
     1561 tggaagataa gatcgccttc ggcaaggaca ccggcgggga cgagcggacg cagctttatc
     1621 tctgctcccc gcgcggggag aacatcgttc aactgacgaa caacccgaaa gtgatttaca
     1681 gcttcggggg gtggtcgcac gacgggaaga agatcgccta tgcggccaac gagcgggatc
     1741 aggcctactt cgacgtctac gtccgggacc tggccatcga ccaggcgaag ttggtgtacc
     1801 agtcggacgc gaacaactct gtggcggcct ggtcgcccga tgacaggtac ctggtcgtgg
     1861 cgcgcgccaa caccaacctg gactttgatc tctttttggt cgaggcggcg accggcacca
     1921 gcacccacct cactccccac acaccgccgg cggtgtacga gagtatcgag tggactaatg
     1981 acggcaaggg gttctacctg gcctccgacc aggaccggga tttcgtcaac ttggcctatt
     2041 acgacgtggc ggaaaagaag ttgacatttc ttgacgacgg gaaaagcgac gtcgacgggc
     2101 tgaaactttc cgatgataac cggtacctgg ccattgtgcg caacatcgac ggctacggcc
     2161 ggctctttct ccgcgaacat cccctgcggg tcgggacgat aacccaggcc aagacgagcg
     2221 aagtgcacct ccccgatggg attccttccg gtttgacttt ttccaaggac ggcaagatga
     2281 tggctttcgc ttttgtcggg ccgacacaga atgggaatat ctggacctac gagatggcgg
     2341 gggagaaatt gacggaggtg accagaagca ccctggccgg gatcccccca tcgtccttcg
     2401 ttgcgccgac tctggtaaag ttcaagtcgt tcgacgggct ggagctctcc gccttcatct
     2461 acctgcccac aggcgccaaa aaagacggct cggcaccttg catcgtgatg gtgcacggcg
     2521 ggccggagtc tcaggagcgg ccctactttg ccaccaccta ccagtactat ctctcccgcg
     2581 ggtacgcaat tctggctccc aacattcgcg gctccaccgg ctatgggaaa acctacacgc
     2641 acttggatga tatcgaaaag cgcggccaag ccatccagga cgtcaaagct tgctataatt
     2701 ttctgaagaa ttccggctat gtcgacccgg ccaaagtggc ggtgatgggg gcaagttacg
     2761 gcggctacat gaccctagcg gcagtcactt ttctgcccga attgtgggcg gcgggggtcg
     2821 atatcgtcgg catcgccaac ttcgagactt ttctgaaaaa caccggcgcc tggcggcgga
     2881 aactccggga ggcggagtac ggttacctcg acaaggatat tgaggtcctc cggaagtatt
     2941 cgcccattca cgcggtggat aaaatcaaat gtcccctctt tgtcatccag ggagcgaatg
     3001 acccgcgggt gccgcagtcg gaagcagagc agatcgtcga ccaggtaaaa aagcagggcg
     3061 gcgcggtgga atacatgctc ttccccgatg aagggcacgg cctggcaaaa ttggccaacc
     3121 ggatcaaggc ctacccggcg gtggcggagt tcttagacaa gcacgtcaag aaccggggcg
     3181 ttgtgcaagc tggagattca agcggcgaaa aggaaaagtg aagcacttga caggtgcgtt
     3241 ctcgtttttt aggtttgtcg cgccttgagg gaaagaggcg aatcgtttgg cgcacgtcag
     3301 ctgaaaggag gaggaatgcg gaaaagtctc ttggtgctcg cggtcggttt gttcgcggtg
     3361 acttcgatcg tggcacagca ggagcagagc aagagcaaca agccggcgga gccgtcgatg
     3421 gagatgatga tgcccaagcc gggaccggag ctcgcaaagc tggattactt tctcggtacc
     3481 tggacgggcg aggaaacgat gaacagcccg ggcatgccgc cggggagcaa gaacaagtcg
     3541 aaaaccgtca ccaagcgcgg aatcggcggc atgttcctgg tgggcgatta cacgtcgtcg
     3601 atgggcgagg ggaagaacgc gatgaagttt gcaggtcact ccctctggac ctatgacccg
     3661 cagaagaagg gttaccggca ctggtggttt gattcctttg gggacggcca ggagacgccc
     3721 ggcaactggg tggacgacaa aacctacgtc ggtgaaactt cctacgaaat gatgggccag
     3781 caggttaagc agcggcagac ctacgccatc ctctccccga cccagttcac catgaagatc
     3841 gagatggaca acggtcaggg atggatgacc ttcctggaag ggacgtacac caagcagacc
     3901 cagaaacccg gtaagtaatc attcacacgg gtggaaagga cgaggaatgc ggaaaattct
     3961 ttgcgtgctc gcactctgtt cgattgcgct gaccgtcgcg gcggcgcagc agcaggagaa
     4021 aagcaaatcc gggcagccgg aagggcagtc gatggggatg atgatgccca aaccgggccc
     4081 ggaattggcc aagctcgact acttcttggg cacctggacg ggcgaggaga cgctcaatta
     4141 cccgggcatg ccggcaccgg ccaagagcaa ggcgaagtac acggccaaac gggggattgg
     4201 cgggatgttc cttatgggcg actacaacgc caccatgagc gatgggaaaa ctgaacagaa
     4261 gatggaagcc cacacgctct ggacctacga tccccaacag aaaaattacc ggctgtgggg
     4321 attcgactcc tttgggaccg cgatggagat gcgcggcaac tggaccgacg acaaaacctt
     4381 tgtggcggaa acctccaccg agatgggcgg catgccggtc aagctgcggg acacctacac
     4441 gcttgtctcc ccgaccacct gcacgctgaa aagcgaaatg gatatgggcc aggggtggca
     4501 gaccggcatg gaggggacct acactaagca gaccgaaacc aagaaaccca agtaagcggt
     4561 ctttccgtcc gccgccgggc gtctggcgag agatgcccgg tttttcattt gcggagccat
     4621 tttgctggca gccggacgca ggagatcggg tgtctgcgtt tctctttttt cgcttgacag
     4681 cgttcgacga gagttttaga ttttgagccg ccttccgcca gcagcgggag caatgtaccg
     4741 aatggaggaa aactgacagc cacgggttcg ccggtcgcaa tgccaatgga gtcgccacac
     4801 ttcaacaaag tgtggtattt tgttttcgga caatccctat gcggttgaca gctccggtga
     4861 gaattttctt tctggcggta ctcctgtccc tcgcctgggt tttgccggcg tgggctatcg
     4921 cgccgcctct ggacgttcaa gccaaagata cgcccaacga tgccggcaaa agcataaccg
     4981 tcacctggaa aaaatcgccg gacgaccggc ccggcgtggc cggggtggcg gagtaccgta
     5041 tcctgcgcgg cactagtctg gacgggccgt ccacctttgt cggcaccgcc accggcggaa
     5101 cggaactctt caccgacaac acggctcaga ataacatcaa ttactactac aaaatcgttg
     5161 cggtcgcagg cgacaaaagc gaggccgaat cgctccccag cgcgccaacg cacgcctacc
     5221 aacagtggtt caacgccaaa cgggtgaatc tcctgatcgg agtgctgctt ctgtctttcg
     5281 ccatcatcta ttttatcgaa caggccaaaa aagggaagcc gctcttcatc cgcaagatcg
     5341 ccgggctgga agctgtcgac gaagcgatcg ggcgggccac agaaatgggc aagaaagttc
     5401 tctacattcc cggtattcaa gatctggata acgtacagac cgtggccggc gtgacgattt
     5461 taggccgcgt ggccaaagtc gcggctgagt acgaaacaca cttagaagtg cccacgtcac
     5521 gcgcgctcgt catggtggcc tgccgggaag cggtgcagga ggggtactcc ctggccgggc
     5581 ggcccgacgc ttacaatcag gaccggattt actacttgac tgacgagcag ttcggctacg
     5641 ccgctggcgt tgacgggatc atcgtgcggg agaaaccggc ggcgatcttc taccagggat
     5701 gctttttcgc cgagtctttg attctttccg aaacgggcaa ttccatcggc gccattcaga
     5761 tcgcgggcac ggcccagccg acgcagctgc cctttttctt tgccgcctgc gactacacgc
     5821 tcatcggcga ggaactgttt gcggcctccg cttacctctc gcgagagccc cggcaattgg
     5881 gcagcctcaa ggggcaggat atcggcaaag ccttaatcat gctggccgtt ctggctggag
     5941 cgatctggca aacgttagcg gtcttcttcc ctgagacatt tcacgccaac gcggccagtc
     6001 ttttcctgac cgaataaaac tctatgcggc gcgaaattcc cttaatcctc actgccatcg
     6061 tcgggctcac gctcgtgatc cagttcttta ttccgcacga gccgttcaac aagatgcaga
     6121 cctggttcgc cgactgggtg gcgatcatcc aggcttttgc catctggctg ggagcgttga
     6181 accttttggt tgtctcggca gagaaagtct ccaaaaagca gccgggatgg ttctactccg
     6241 tcgtcatcat cgccagtttc tttttgatcg tcatccccgg actcttcttc tccggtgggc
     6301 ggcacttcca ggaggccggg acctccttcg actggctcta cgcccatatg ttcacccccc
     6361 tttcggccac gatgttcgcg cttttggcgt ttttcgttgc ttccgcctcc taccgcgcct
     6421 ttcgcgcccg caataaagag gccacgcttt tgcttctggc cgggattttt gtcatgctgg
     6481 gccgggtgcc catcggggat gtgctcacga attttctgcc ggaaaaaatc aggctctcgt
     6541 cggtggccga ctggatcatg gcctacccgc agttagccgg gcaaagggcc atcatgatcg
     6601 gcatcgcctt agggattgtc tccacctccc tgcggatcat tttagggatt gaccgcacca
     6661 tcgtgggcgg aaagtgatgg cagaagttcc gaacgttccc gaaacgcagc gcgggttctt
     6721 cgaccgactg gtggcaatcg accggcgggt catttttctt tttgtcgggc tggccctcct
     6781 cgtacccttg tttatgaacg tttcccagaa gatgaaaatt tccccggaag tgcaatcgct
     6841 cttcgacgcc atgcaagccc tgccgccggg gtccaaagtt ttgctcgcct gtgattacga
     6901 cccaccctcg tcgccggaac tgcagccgat ggcggtcacc gccgtcaagt actgtttcct
     6961 gtaccggctt aaaatgatcg tcattggtct ctggccgcag gggccgcagc aggccgattt
     7021 ggccatccgg gcagcgctga aggatccgga ggtagcgaag gtaaatcccc aatacggaat
     7081 cgactactgc aatttgggtt ttcaggccgg caacgaagtg gtgatccaga agatggggtc
     7141 ggatattccg ggtacgttcc ccaaagactg gcggggaacc gacgtgaaaa cgctccctat
     7201 catgaccggg atcaagaact tcagcaacat cgatttcatc ttcaacctct cggcgggata
     7261 ccccggaacg cgggagtggg tgcagttagc cgccgaccgc ttccacgcca aactcgtttc
     7321 cgccgccacg gcggtgcaga cgccggagta caccccgtat ctggggcggc aactcctggg
     7381 catcttgggc gggatgaaag gaggagcgga gttcgaggcg ctcactactt accgcgggaa
     7441 ggcaaccaag ttcatgctgg cgcagtcgct ctcgcacctg atcgtggtgg cctttatcat
     7501 catcgggaac gcagcctact tcgccagccg gaagaagaaa ggcgcatagg gcatggaagg
     7561 cttcaccttc ggtaccaccc tcgcggccat cctcaccatc gggattctct ccttcctcta
     7621 taaggataat ccgctctaca aactctgcga gtcgattttc atcgggattt cggccggcta
     7681 ctggttcgtt tccctctact tccagaacat gctgcccaaa atgttcgaca atttgggcat
     7741 aaccaaagcg ctcggctcca aagaagcagg cgatggggca ctgcaggttc ttttgcacca
     7801 acaccactgg gatgagcgct ggattttcct ggtggccggc gttttgggca tcatgatgct
     7861 cctgcgctta tttcccaaaa tcggctggat ttcccggtgg cctctggcgt ttgtcgtcgg
     7921 ctacacctcc ggactttatt tcatcgccta cctgcagtcg aacgttttcg cccaggtgca
     7981 gaagtcggtc gtacctttgg ccacgctggc ccagtcgaac ggtgccattg cctgggggcc
     8041 gacgatcgga actctggtgg tcgtcttcgg aactctggcc ggtctggtct acttcttttt
     8101 ctccaaagag cataaagggt ttttcggcgg aacggcgcgg ctggggatct ggtttctgat
     8161 ggt
//
LOCUS       DBNGZD010000377         9174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1865099, whole genome shotgun sequence.
ACCESSION   DBNGZD010000377 DBNGZD010000000
VERSION     DBNGZD010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1865099"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="AC16GM_09935"
     CDS             <1..1137
                     /locus_tag="AC16GM_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR2924027.1"
                     /translation="LGRIQFVREASLFTQEEIDFACATAELLALKLLEESHQKVLDDN
                     RRLREALQSTCAFPNVITAHAGFTEMLKRLDQVKDSTLSLLLEGETGTGKDLLAKAIH
                     YSSARRDKPFVSVNCAALPETLLESELFGHRKGAYTGADRDKIGLFAEADGGTFYLDE
                     VSELPPAIQVKLLRVLEEKEVTRLGDTRPQKVDVRVISAGNKDLKTQMEKELFRADLY
                     YRLCTLTFKLPPLRERKEDLPLLVEHFVKKHSPAGATTLPRIDPAFLRLLTEYDWPGN
                     VRELENEIRKVLLLLGEGTRLTPDLLSGKFFGPDGDGLGKTTNGSFSLYEHISQFERR
                     YLLKALSENRWIKKRAASALQIPESTLRVKMKQYGLQEEKETET"
     gene            1361..2287
                     /locus_tag="AC16GM_09940"
     CDS             1361..2287
                     /locus_tag="AC16GM_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924028.1"
                     /translation="MKKNLIVLVIGEAFLVFTTSWLSSASTSTTTVSPQKYVRGDALT
                     NKSGSGIENRRLYRVDALTSSRVLIYEAEGDFISDILSSPNGKFIGFLEGKNQMRSLV
                     IIRPEGQVADRLKSNIHKFSWSPTSGKVAFIQGVTSEGRWVIPQGVAIYDLMTKSIKK
                     IADSALFLNWAIFDSNIYYSWNQRYYRYETKTGITEIVPYLGIDFSPDGKYYLSSHPD
                     ESFQVFERERNMNITDRFYKRFKHTPIRPTWYGGDSHLLLITYREVITDPKYENRPAK
                     PIIGIREYHYYLYDIEKDSTVKEWREKPLQKE"
     gene            complement(2433..5099)
                     /locus_tag="AC16GM_09945"
     CDS             complement(2433..5099)
                     /locus_tag="AC16GM_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HIR2924029.1"
                     /translation="MGRLVVGLVFLSLVCSGGGFLAAEAAPPHPRFKERLLATGEYEA
                     FLGKLAQARQEGWDQPNPQPFRLSPLALAGLAVDTLKVVVLLVDFSDNQMTAGFANAN
                     PDTIQKILFSRGYIASGSMAEYYDEVSLGKLYVTGQVFGPFHMDSTYAYYVNNNGGLN
                     NTCPRCARQLTRDAIAKADTAVNFSQFDGNGDGRVDGLIIMHAGQGAEEGGISSRIYS
                     HFSNTGFPADSADGKSIPFYIAAPEEAGYAFPAPGLVTIGVPTHEFGHNLGLPDLYDV
                     DDVVNGRWRGLGDWSLMASGNYLGDGIYSGRKPGHLDPWSKIRLGFAAVDTVRTNRSA
                     VPIPAVETSEKIFRLWIFGGTGPQYFLVENRRKKLFDTDLPGSGLLIYHVDETVIDIN
                     LNPYNGNPSHPFIVVEQADGRRQMETDSSDGDAGDPYPGTTNHRTFDDLSNPNSRNYL
                     NQSTQVEVLNVSNSADTMAADLNVRWSRPLFQFSGTSVNDSINGNPAPNLRADSGETV
                     ELMITLKDLQASTDSVRLRLRSQDTLVSVSDSVGFIPAIAGSGGQASNTGDRFVVTID
                     PRTGRSRLVNFLLCAWSKDSSITLDTVFHLAVGPTDILIVDDDTGKTYETYYEAPFDS
                     LRIPYDVWHKKTQGRVDSLRLRQYPKVVWFTGDRRCTATCTRPLTVNDIIDLKAYLNT
                     GGKLFLTGQDIAQSLAGTADSTFLRDYLHCRYFSTGFPFAARGVLGDPVTGFIDSVYF
                     GAPPDGAGNQQSPDLLTPGDSASAVFKYTPSGQVAGTRFKGQWQTVFFAFGFEAISST
                     RSGYNTRMDVMNAVMNWFDTGAVCALLGDASGDRVVDIVDLVVLVNYVVFGSPIPTGI
                     NCTELTGDGVIDILDIICLLNYVVFGNPIPCG"
     gene            5242..7524
                     /locus_tag="AC16GM_09950"
     CDS             5242..7524
                     /locus_tag="AC16GM_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2924030.1"
                     /translation="MPLIRRPISREVERLFLVLLFILALGLRLLYLFEVAQSNPYFAH
                     PTLDAGYHDDWAQSIAGGNLIGSGVFFRAPLYPYLLGFVYAVFGHDYFAARAVQHTLG
                     ALAVILIFFLGRRLFNRTVGALAALLAAFYPILIYFEDELLLDWLLVFLGTALFLLLY
                     RAKEKPAAGRWLVAGLMFGFACITRPTFLPFFVCVIGWIFYLFWPQKNWPGIARSAIL
                     FSLGTATIIAPVSVRNWFVGHDFVLIASQGGTNFYIGNNPAADGHTSRLPEPFGVTWE
                     YRDLQYPVDQYLGRPAKPSEVSDYWYREGWRFIRENPRAALQLTLKKFYLLWNKFEIP
                     NNQNYYTFARIVPLFRWLPLGFWFVGPLGLLGLVLAWRAKRARLVALFVLVYTLTTVA
                     FFVCDRFRVPLLPFLFIYAAYALVALFRLLQAETARSLVMPAALLALLILFANSNAYH
                     FQKENPAQEWDRQGNLQLRLGRYREAEDYFRKTLEAQPDFPDAHLNWGVALWKLGRVE
                     EAVFHFRREAELHKASEKPYANLSTIYRLRGQSDSAIVFARQAIQRRPYAPAGYLNLA
                     QAYEEKNDLVRAESTLTAGQARCGGEFTYGNYLLAGVLYQQGKFGLASAAYRPVLKEL
                     GQKTRPAYDPESIFGPEGKTKDLAARLEARAWYGLGASLGALGQLDSAITAFKRALVL
                     WPGFADAQADLGVAFQKQGMAAEAIEALQKAIALDPGKPIYYYNLGVSLATTGRREEA
                     RKNFQKTLELDPSFQPARVH"
     gene            complement(7642..>9174)
                     /locus_tag="AC16GM_09955"
     CDS             complement(7642..>9174)
                     /locus_tag="AC16GM_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924031.1"
                     /translation="LTTDSLYRLAAREVSRVVANPPRWTVVDNINANGSRVAVKLVSL
                     PNGVFVDQVLFLRAFDNFNPPASTPVLYRRYYRNNHPPNTSIEARSDTLFFDRIDTTV
                     VNYCLPETTATWKGINIGWVGSDDIDFPKGGAPLDFSWELYGPFAAKQDAKIDAGKKL
                     DSSYNATTQDPWVTATTRRFVNLQTGWYFFVVRSRDDALVPDTTPATGRFRIIRPTFE
                     RPMLLLTYDNRLNPLIQDFGNINAADSALVRNFYYRLLSGSGYLWDAGTDHFVVKRAE
                     GNDSIAFFEEKLSHYQLVVLLYEGIKEPYLNATWTKLFTYLDAGGKVWAMGRNILQYP
                     FEGPFTVPTLKCTDLKTGKPFTYFNLECQYYATSINLGLPGAGKPVNNQFVGAGSLRS
                     ELPGLSVDLAKLFRYGTRTAGGRVMWDSTRMPDVNYDQRGTKATPLYVFQVPVAFADT
                     SHLGGTVVAVRYNGPPWQGKPIFKTAHFGFPLYVLDDSGGQVQAMVTEMLRWFLNPAA
                     IP"
BASE COUNT         2071 a         2574 c         2532 g         1997 t
ORIGIN      
        1 ctgggccgta tccaatttgt gcgggaagca agtcttttta ctcaagagga gatcgacttc
       61 gcctgcgcca cggccgagct tttggcgctc aaactcctgg aggagtcgca ccagaaggtt
      121 ctggacgaca accgccggct ccgggaagcg ctgcaatcga cctgcgcttt ccccaacgtc
      181 atcaccgccc atgcgggatt taccgaaatg ctcaagcgtc tcgaccaggt gaaagattcc
      241 accctgtcgc ttctcttgga aggcgaaacc ggcaccggca aagacctcct ggcgaaagcg
      301 attcactact cgagcgccag acgggacaag ccgttcgttt cggtgaactg tgcggcgttg
      361 ccggaaacgc ttctggagag cgagctcttc ggccaccgca aaggggccta caccggtgcg
      421 gatcgtgaca agatcggcct ctttgccgaa gccgacggcg gcactttcta cttagacgaa
      481 gtttccgaac tgccccctgc gattcaggta aaactcttgc gggttttgga agaaaaagaa
      541 gtgacccggc tgggcgacac ccggccccag aaagtcgacg tgcgggtcat ctccgccggg
      601 aacaaagacc tgaagacgca aatggaaaaa gagcttttcc gggccgacct ctactaccgg
      661 ttgtgcaccc tcacgttcaa acttccgccc ctgcgcgagc ggaaagaaga ccttcccctt
      721 ttagtcgaac actttgtgaa aaaacactcc cccgccggtg ccacgactct cccgcgcatc
      781 gaccctgcct tcctgcggct tttgacggaa tacgattggc cgggcaacgt ccgcgagttg
      841 gagaatgaaa tccgcaaagt gcttctgttg ctcggcgaag gcacccgcct gactcccgat
      901 ctgctttcgg ggaaattctt cggcccggat ggcgacgggt taggcaaaac aaccaacggc
      961 tccttttccc tctacgagca catatcccag ttcgagcgcc gctacctcct caaagccctc
     1021 tccgaaaacc gctggattaa aaaacgcgcc gccagtgccc tccagatccc cgaatcaacc
     1081 ctgcgcgtca agatgaagca gtacggcctg caggaagaaa aagaaacgga aacctaagct
     1141 aagataaaaa cggggtaaaa ggccagtgca tgaggaagtt tctggccaca gtttccgtag
     1201 tagttctttc ggctgtgtat tcggtgtgag ccgcaaagag aggatagaga ccgtttcctt
     1261 ttttgaaaaa gattgacaag tgccgggggt gggaaaactt aactaacatc tcctattcaa
     1321 attagagaca acgctggtac aggaaccaaa agggataaat atgaagaaaa acttaattgt
     1381 tctggtaatt ggagaggctt ttttagtttt caccacatct tggctttcca gtgcatctac
     1441 ttcaacaacc accgtttctc cgcagaagta tgttcgcggt gacgccctaa cgaacaaatc
     1501 aggaagcggg attgagaatc gtcgtctcta tagagttgac gcgctaacat cttcaagggt
     1561 tttgatttac gaagcggaag gggatttcat tagtgacatt ttgtcttcgc ctaatgggaa
     1621 atttattggt tttctcgaag gtaagaatca aatgcgttca ttggtcatca taagaccgga
     1681 gggacaagta gcggatagac taaaatctaa catccataaa ttttcatggt caccgacttc
     1741 aggcaaagtg gctttcattc agggtgtaac ttcagaaggt agatgggtga ttcctcaagg
     1801 cgtggcaatc tatgacttga tgacaaaatc aattaagaaa attgctgact cagcattgtt
     1861 tctcaactgg gctattttcg atagcaatat ctactattcg tggaatcaga ggtactaccg
     1921 gtatgagaca aaaactggaa ttactgaaat agttccctat ctcggtatag acttttcacc
     1981 agatggaaaa tactacctct catcacatcc agacgaatct tttcaggtat tcgagcgaga
     2041 acgcaatatg aatattacgg atcgctttta caaacgtttc aagcacacgc ctattcgacc
     2101 gacctggtat ggcggagaca gtcacctttt gctgataacc taccgtgagg taataactga
     2161 ccccaaatat gaaaatagac cggctaaacc catcattggg attcgcgaat atcattatta
     2221 tctctacgac attgaaaaag attctaccgt caaggagtgg cgcgagaaac cgctgcagaa
     2281 ggagtgaccg gacgctctat ctgaaactat aatagtgcca ctaagtatcg tctttaccgc
     2341 atcagctact tttcttcagt aacacctcca ttgtttcaac gaaatctcct gcccaaagtc
     2401 agtaaaaaag cgctctccgc cggccccctg cctcatccgc agggtatcgg attcccaaag
     2461 acgacgtaat tgagcaggca gatgatatcc agaatgtcga tgaccccgtc gccggtcaac
     2521 tcggtgcagt taatgccggt ggggatcggt gacccaaaca ccacgtagtt gaccaggacg
     2581 actaaatcca caatatccac caccctgtcg ccgcttgcgt cacccaaaag ggcgcagacc
     2641 gcgcccgtat caaaccagtt catcaccgca ttcatcacgt ccatccgggt gttgtaaccc
     2701 gaacgggtgc tggagatggc ctcgaacccg aaagcgaaaa agaccgtctg ccactgcccc
     2761 ttgaaacggg ttcccgccac ctgccccgag ggtgtgtatt tgaaaaccgc gctggcggaa
     2821 tcacccgggg tgagcagatc gggactttgc tggttcccgg ccccgtccgg cggagcgccg
     2881 aaataaactg agtcgatgaa cccggtgacc ggatctccta ataccccccg ggccgcaaag
     2941 gggaagccgg tggagaagta ccggcagtgg aggtagtcac gcaggaaggt cgaatcggca
     3001 gtcccggcga gactctgggc gatatcctgc ccggtgagga agagcttgcc gccggtattc
     3061 aggtaggcct taaggtcaat gatgtcgttg acggtcaaag gacgtgtgca ggtagccgtg
     3121 cagcggcggt ccccggtgaa ccagacaacc ttcgggtact gcctgagccg caagctatcg
     3181 acccggccct gcgttttctt atgccacacg tcgtacggga tcctcaaact gtcgaaggga
     3241 gcttcatagt acgtctcgta cgtcttgccg gtgtcgtcat caacgatcaa gatgtcggtc
     3301 ggcccgaccg ccaaatgaaa cacggtgtct aaggtgatag aactgtcttt gctccatgcg
     3361 caaaggagaa aattaaccag ccgcgatctg ccggtgcgcg ggtcgatggt gacgacgaat
     3421 cggtcgcccg tattgctggc ctggccgccg ctgccggcga tggccgggat gaagccgacg
     3481 ctgtcactca ccgagacgag cgtatcctgc gaacgcaggc gcaggcggac gctgtcggtt
     3541 gaggcctgga ggtctttcag cgttatcatc aattcgaccg tctcgcccga atcggccctc
     3601 aggttgggcg cgggattgcc gtttatggag tcgttgacgc tcgtgcccga gaactggaac
     3661 aaaggccgcg accacctgac gttcagatcc gcggccatcg tgtcggcgct gttggaaacg
     3721 ttcaaaactt cgacctgggt cgactggttc aggtaattcc ggctgttggg attggagagg
     3781 tcgtcaaaag tgcggtggtt cgtggtgccg ggatacggat cgcccgcgtc gccgtcactg
     3841 gagtcggtct ccatttgccg acggccgtcg gcctgctcaa cgacaatgaa tgggtggctg
     3901 gggttgccgt tgtaggggtt aaggttaata tctatcaccg tctcatcgac gtggtaaatt
     3961 aaaaggccgg agccggggag gtcggtatcg aagagcttct tgcggcggtt ttccaccaaa
     4021 aagtactgcg gtccggtccc gccgaaaatc cagagccgga aaattttttc ggaggtctcg
     4081 acggcgggaa taggcacggc cgaccgattc gtccggaccg tatccactgc ggcaaatccc
     4141 agccggattt tggaccaggg atcgaggtgg cccggcttgc ggccgctgta gattccgtct
     4201 cccaggtaat tgccggaagc catgagcgac cagtcgccca acccccgcca ccggccattg
     4261 accacatcat cgacatcgta gagatcaggc aatcccaagt tgtggccaaa ttcatgagtc
     4321 ggcacgccga tggttaccag cccgggagcc ggaaaggcat acccggcctc ttcgggtgcg
     4381 gcaatgtaga agggaattga tttaccatct gcagaatccg ctggaaatcc ggtattagaa
     4441 aagtgcgaat agatgcggct ggagatgcct ccctcttccg ctccctgtcc ggcgtgcata
     4501 atgattagtc cgtccactcg accatcgcca ttaccatcaa actgagagaa attgaccgct
     4561 gtatcggctt tggctatggc gtcccgcgtc aactgccgtg cgcatcgggg gcaggtgtta
     4621 ttcaaacctc cattgttatt gacgtagtat gcgtaggtgc tatccatgtg aaaaggaccg
     4681 aacacctgac cggtaacgta gagtttgccc aaagaaactt catcgtagta ctccgccata
     4741 cttccggagg cgatgtaacc gcgggagaaa agaatttttt ggatcgtatc gggattggca
     4801 ttggcgaacc ctgcggtcat ctggttgtcg ctgaaatcga ctaagaggac gacaactttg
     4861 agggtatcca ccgccaaccc ggccagagca agggggctca accggaaagg ctggggattg
     4921 ggttgatccc acccctcctg gcgcgcctgt gccagcttgc caaggaaggc ttcgtattca
     4981 ccggtggcca gcaggcgctc tttgaagcgc gggtgcggcg gcgccgcctc agccgccaga
     5041 aaaccacccc ccgaacagac cagggaaaga aaaaccaaac ccacaaccaa cctacccatt
     5101 accgcctcca ccgcaaactg gagaaatgac aaagcgccgg acaccggatg gctacaggta
     5161 taatataata cgcaaaagtg attcccggag agcgaatttt gaaccattga ggccggggaa
     5221 cccggccata tattggcggt gatgcctctg atacgccggc ctatttccag agaagtggaa
     5281 cgactcttcc tcgttttatt gttcattctg gcactgggct tgcggcttct ctatcttttc
     5341 gaagttgccc aaagtaatcc ctacttcgcc catcccaccc tggacgccgg gtatcacgac
     5401 gactgggcac agagtatagc agggggcaac cttatcggca gcggagtctt cttccgggcg
     5461 cccctctacc cctacctcct gggcttcgtc tacgcggtat tcgggcacga ctattttgcg
     5521 gcgcgggctg ttcaacatac cctcggtgcc ctggccgtca tcttgatctt ttttttgggg
     5581 cggcggctgt tcaatcgaac cgtgggggcg ctggccgcgc ttctggcggc tttttacccc
     5641 atcttaatct atttcgagga cgaactcctg ctcgactggc ttttggtctt tctcggcacc
     5701 gccctgttcc tccttctcta tcgtgcgaaa gaaaaacctg cggcagggcg ctggctggtt
     5761 gctggcctca tgttcggctt cgcttgtata acccgaccga cattcctccc tttctttgtg
     5821 tgcgtcatcg gctggatttt ttatctgttc tggccgcaga aaaactggcc aggcattgcc
     5881 cgctccgcca tcttgttttc tctggggacg gccacgataa ttgcgccggt gagcgtgcgg
     5941 aactggtttg tgggtcacga tttcgttttg atcgcctcgc aggggggaac gaacttctac
     6001 atcggcaaca acccggctgc cgacggccat acctcccgcc tgccagagcc gttcggggta
     6061 acctgggagt accgcgattt gcagtatccg gtggatcagt atttgggccg cccggcaaag
     6121 ccgtctgaag tttcggacta ctggtaccgg gagggctggc gcttcatccg ggaaaatccc
     6181 cgagccgccc tgcaattgac gctgaagaaa ttctacctcc tctggaacaa atttgaaatt
     6241 cccaacaatc agaactacta tacttttgcc cggatcgtcc cgctgttccg ctggctgccg
     6301 ttgggttttt ggttcgttgg cccgctggga cttttgggtc tcgttctggc ctggcgggcc
     6361 aaacgggcac ggctggtagc ccttttcgtt ttggtctaca cgctcacgac ggtcgccttc
     6421 ttcgtctgcg accgcttccg cgttccgctt ttacctttcc ttttcatcta cgccgcctat
     6481 gccctggtgg cgcttttccg gttgctccag gcagagactg cccgctcgct ggttatgccg
     6541 gccgcacttc tggcgctcct gatactcttc gcgaattcga atgcgtacca cttccaaaag
     6601 gaaaatccgg cgcaggaatg ggaccggcag ggcaatctcc aactccgcct ggggcggtat
     6661 cgggaagccg aagactattt ccgaaaaact ctggaggcgc agccggattt ccccgatgcg
     6721 cacctgaact ggggggtggc gctctggaaa ctgggccggg tggaggaggc agtcttccat
     6781 tttcgccgcg aggcggaact ccataaggcc tcggaaaaac cgtatgccaa cctctcgacc
     6841 atttatcgcc tgcgcggcca gtccgattcg gccatcgttt ttgcccgcca agccatccaa
     6901 aggaggccgt acgctcccgc cgggtacctc aatttggcgc aggcgtacga agagaaaaac
     6961 gacctcgtcc gagccgaatc gaccctcact gccggtcagg cacgttgcgg cggggagttc
     7021 acctacggga actaccttct ggccggtgtg ctctatcaac aggggaaatt cgggctcgcc
     7081 agtgcggcgt accggccggt gctgaaggaa ttggggcaga aaacccggcc ggcttacgat
     7141 ccggagagta tcttcgggcc cgaaggcaaa acgaaagatc tggctgcccg gctggaggcc
     7201 cgcgcctggt atggcctggg cgccagcctg ggagcgctgg gacaactcga ttcggccatc
     7261 accgcattca aacgggcact ggtgctctgg ccgggatttg ccgacgccca ggccgactta
     7321 ggggtggcat tccaaaagca ggggatggct gcggaagcca ttgaggcact ccagaaagcg
     7381 attgctcttg acccggggaa accaatttat tactacaatc tcggcgtgag tctcgctacg
     7441 accggccgcc gggaagaagc gcggaaaaac ttccaaaaga ctttagaact tgacccatcc
     7501 tttcagccag cgagagtaca ctgaccggcc ttgcgatagt tcctcaacac ataacgaaca
     7561 acttaagcag aatcgctgct gactaaccac caaaccaaca gccccgcctc tggcggggct
     7621 tttgcttccc agaaagttac ttcagggaat agccgcggga ttcaaaaacc accgcaacat
     7681 ctctgtaacc attgcctgca cctgaccccc gctatcgtcg agcacgtaga gcgggaaccc
     7741 gaagtgggcg gttttgaaga tcggcttacc ctgccagggc ggaccgttgt agcgcaccgc
     7801 caccaccgtg ccgccgaggt gcgaggtatc tgcaaaagcc acggggactt gaaagacgta
     7861 taagggcgtc gctttcgtcc cgcgctggtc gtagttcaca tcgggcatcc tcgtggaatc
     7921 ccacataact cttcctcctg cagtccttgt tccataacgg aacagtttgg ccagatcaac
     7981 cgacaagccg ggaagttcag accgtaggga gcctgcgcca acgaactgat tattgacagg
     8041 tttccctgcc cctggcaagc ccagattgat cgaggtcgca tagtactggc attccaggtt
     8101 gaagtaagta aacggcttgc ccgtcttcag atcggtgcac ttcaaagtcg gaacggtaaa
     8161 tggcccttcg aaagggtact gcagaatgtt ccgtcccatc gcccaaacct tgccgccggc
     8221 atccagatag gtgaaaagtt tcgtccaggt ggcgttcaga tacggttcct ttatcccctc
     8281 gtacaacagc acgactaact gatagtgaga gagcttctct tcaaagaaag caatgctgtc
     8341 gttcccttcc gctctcttca cgacaaagtg atcggtgccg gcatcccaga ggtacccgct
     8401 tccggagaga agccggtagt agaaattccg gacgagcgcg ctatccgcag cgttaatatt
     8461 tccaaaatcc tgtatcaagg ggttcagacg gttatcgtag gtcaataaga gcataggccg
     8521 ctcaaaggtt ggcctgataa tccggaaccg tcccgtggcc ggcgtggtgt cggggaccaa
     8581 agcatcgtcc cgtgaccgga caacgaagaa gtaccagccg gtctgcaggt tgacgaagcg
     8641 tctggtcgtg gcggtcaccc aggggtcctg cgtggtggcg ttgtaggagg aatcgagctt
     8701 tttgcccgca tcgattttgg catcctgttt ggcggcaaac gggccgtaga gctcccagga
     8761 gaaatctaaa ggtgcaccgc ctttgggaaa gtcgatgtcg tctgagccga cccagccgat
     8821 attaatcccc ttccaggtgg cggtggtttc cggcaggcag tagttgacca cggtggtgtc
     8881 aatccggtca aagaacaatg tatcacttct cgcctcgata ctcgtattcg gcgggtggtt
     8941 gttgcggtaa tagcggcggt agaggaccgg ggtgctcgcc ggcgggttaa aattatcgaa
     9001 ggcgcgcagg aagaggacct ggtcgacgaa tacgccgttg ggcagcgaaa ctaatttcac
     9061 ggccacccgc gagccgttgg cattgatgtt gtctaccaca gtccagcggg gggggttggc
     9121 caccacccgg gaaacttccc gggcggccaa acggtacagg gaatcggtgg tcag
//
LOCUS       DBNGZD010000378         2787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1170947, whole genome shotgun sequence.
ACCESSION   DBNGZD010000378 DBNGZD010000000
VERSION     DBNGZD010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1170947"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..>2787)
                     /locus_tag="AC16GM_09960"
     CDS             complement(29..>2787)
                     /locus_tag="AC16GM_09960"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2924032.1"
                     /translation="YKGSVGFAVLFVLLSVAVISAQPAQPTKTPSLPSLKFEKYVLPN
                     GLQVILHQDHSIPVVAVNVWYHVGSKNEKLGRTGFAHLFEHMMFQGSEHQPGEYFAPL
                     EKVGGSANGSTAEDRTDYWENVPANYLEMALWLEADRMGYLLPAMDQKKLDNQRDVVK
                     NERRQGVDNEPYGKAEEMIPSLLYPMGHPYSWSVIGSHEDLTRASLDDVKDFFRNFYT
                     PNNASLCIAGDFDPKTAKELVKKYFGDIPPGPSVERLKNWVPTLDGEKRIVVPDRVSL
                     PRLYLLWQTTPFYTPGDAELDLLGNILSSGKTSRLYKSLVYEKQIAQDVAAYQASREM
                     GSNFAVVVTAKQGHTLEEIQKAVDQELAAIRTTGVTPAELIQAQNNFEANFMRQLQNV
                     GSFGGRADLLNQYNTYLGDPGKLQWDLDRYLKATPEGVKQVAEKYLDPKRRVLLTVVP
                     QEDYQPEAVTVDRSTQPPAGPEPTFTPPKVQRAKLSNGMDVLLVEEHKLPLVQVELVL
                     KSGWAADPKERPGAASLTAELLDEGTTTKNALQISGEVKSLGAQLGTGSFFDASTASI
                     NLLKKHLDKGLSILSDVIMHPTFPAEELERQRQIYLGRILQESKEPFTSGFKVFLRTL
                     YGPGHPYGQPYTGSGTETSIKAIGRSDLTKYYQTYYAPNNAALVVVGDITLAEALPKL
                     EAAFKEWKPKEVPKVEIPPVKPLTAPKVFIVDKPGAAQSVILMGNFALARKAPDYIPA
                     QVMNNVLGGQFTSRINSNLREDKGYSYGTFSFFFTARGIGPFVTFAPVQSQSTKEAVA
                     ELEKELKAIRETKPVTADELKQSKDNLIKGYSQNFQTLGAIAGQVAEIVMYDLPDDEI
                     SRYPAEVAAVDASAAQATAKKYITPDGMLCVIVGDKSKIEAGIRELGVGEIGYLDPEG
                     EETK"
BASE COUNT          501 a          827 c          816 g          643 t
ORIGIN      
        1 cgcgccgccc cccgacaccc tttcgcttct acttcgtttc ttccccctcc gggtcaaggt
       61 agccgatctc gcccactccc agttcgcgga tacccgcctc gattttcgat ttatcgccca
      121 caatgacgca gagcattccg tccggggtga tgtatttctt cgccgtggcc tgggcagcgc
      181 tggcatctac ggccgcgact tccgccgggt agcgggagat ttcatcatcg ggcagatcgt
      241 acatcacgat ttccgccacc tgtccggcga tggcgcccaa cgtctggaag ttctgggaat
      301 agcctttaat caggttgtcc ttactctgtt tcaattcgtc ggcggtgacc ggcttcgtct
      361 cccggatggc tttcaattcc ttctccaatt cggccaccgc ctctttggtc gattgcgact
      421 gcaccggcgc gaaggtcacg aagggcccaa tgccgcgcgc ggtgaagaaa aaggagaacg
      481 tgccgtagga gtaccccttg tcttcccgca aattcgaatt gatgcggctg gtgaattgcc
      541 cgcctaagac gttattcatc acctgcgccg gaatgtagtc gggcgccttc cgcgccaggg
      601 caaagttgcc catcaaaatc accgactgcg ccgctccggg cttatcgacg atgaagacct
      661 tgggggcggt gagcggcttg accggcggga tctccacctt gggcacctct ttcggtttcc
      721 attctttgaa agcggcctct aacttgggca gcgcttcggc gagcgtgatg tcgcccacca
      781 ccaccaaggc cgcgttgttc ggcgcgtagt aagtctggta gtatttggtt agatcggaac
      841 gcccaatggc cttgatcgaa gtttcggtac cgctgcccgt gtagggctgg ccgtaggggt
      901 ggcccggccc gtagagcgtg cgcaggaaaa ccttaaagcc gctggtgaac ggctccttcg
      961 attcctgcag aatgcgcccc aggtaaatct gccgctggcg ttccaactct tccgccggga
     1021 aggtcgggtg catgatcacg tcggaaagaa tcgaaagccc tttgtctaag tgcttcttga
     1081 gcaggttgat gctggcggtg gaagcgtcaa agaaactgcc ggtccccagc tgggctccca
     1141 aacttttcac ctcaccggaa atctggagcg cattcttggt cgtcgtcccc tcatctaaga
     1201 gttcggcggt gagactggct gcgcccggac gttcctttgg gtcggccgcc cagccagact
     1261 tgagcaccaa ttccacctgc accagcggca gtttgtgctc ttcgactaaa agaacgtcca
     1321 tcccgttgga gagcttggcg cgctgcacct tgggcggagt gaaggtcggc tccggccccg
     1381 ccggcggctg agttgagcga tcgaccgtga ctgcttccgg ctgatagtct tcttgcggca
     1441 ctacggtcag gagtacgcgg cgtttcgggt cgaggtactt ctccgccacc tgcttgaccc
     1501 cttccggcgt cgccttgagg taccggtcga ggtcccactg gagtttcccc ggatcgccta
     1561 agtaggtgtt gtactggttc aaaaggtcgg cccgtccgcc gaaactgccg acgttctgca
     1621 gttgccgcat gaaattggct tcgaagttgt tctgcgcctg gatcagttct gccggagtca
     1681 cgccggtggt acggatggcc gccaattcct gatcgactgc cttttgaatt tcctccagcg
     1741 tgtgcccctg cttggcagtg acgacgacgg caaaattgct gcccatttcg cgcgaggcct
     1801 ggtaagcggc cacatcctgg gcaatctgct tctcgtagac cagcgatttg tataatcgtg
     1861 aagttttccc ggaggaaagg atgttcccca gaagatctaa ttcggcgtcg ccgggggtgt
     1921 aaaacggtgt ggtctgccac aagaggtaaa gccgcgggag cgagacccgg tcgggcacca
     1981 caatccgttt ctccccgtcc agcgtcggaa cccagttttt cagccgttcc acggaagggc
     2041 ccggcggaat gtcaccgaaa tattttttga ccaattcctt ggccgttttc ggatcaaaat
     2101 cgcccgcaat acagagactg gcgttgttgg gagtgtagaa gtttcggaag aaatctttca
     2161 cgtcatccag actggcgcgg gttaggtctt cgtggctgcc gatcaccgac caggaatagg
     2221 ggtggcccat cgggtagagc agagacggga tcatctcttc ggccttgccg tacggctcgt
     2281 tatcgacccc ctggcgcctc tcgtttttca ccacgtcgcg ctggttgtcc aatttctttt
     2341 gatccatcgc cggcaacagg taccccattc ggtcggcttc cagccagagc gccatttcaa
     2401 ggtagttggc gggcacgttc tcccagtagt cggtgcggtc ttcggcggtg gagccgttgg
     2461 cggagccgcc cactttttcc agcggcgcga agtactcgcc cggctggtgc tcggagccct
     2521 ggaacatcat gtgctcgaac aggtgggcaa acccggtgcg gcccagcttt tcgttcttgg
     2581 agcccacgtg gtaccagacg ttcaccgcca ccaccgggat cgagtggtct tggtgtagga
     2641 tcacctgcag accgttgggc aaaacgtatt tttcgaactt cagcgagggc aagctgggtg
     2701 tcttagtggg ttgcgcgggc tgtgctgaga taaccgcgac actcagcagg acgaacagaa
     2761 ctgcgaaacc gactgaacct ttgtacc
//
LOCUS       DBNGZD010000379         4207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_338259, whole genome shotgun sequence.
ACCESSION   DBNGZD010000379 DBNGZD010000000
VERSION     DBNGZD010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_338259"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /gene="trpB"
                     /locus_tag="AC16GM_09965"
     CDS             complement(<1..1221)
                     /gene="trpB"
                     /locus_tag="AC16GM_09965"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR2924033.1"
                     /translation="MASVKALKSQRYRWPDKGGHFGKFGGMFVPETLVTPLLELWDGY
                     WKARRDREFQSELRGLLAHYAGRPTPLYFAERLSGKLGLRIYLKREDLTHTGAHKINN
                     TVGQILLAKRLGKARIVAETGAGQHGLATATVAAKFGLECTIYMGAEDVQRQALNVFK
                     MKLLGAQVIPVTSGSRTLKDAINEAIRDWVTNVRTTHYRLGSTVGPHPYPTIVRDFQS
                     VIGRETKKQIIGQAGRLPDLIFACVGGGSNALGMFYPFLTTKVRLVGVEAAGLGLNTK
                     KHAATLNRGKAGILHGSLSYLLQNGNGQVQVAHSISAGLDYPGVGPEHSFLKESGRVE
                     YTAVTDAEALEAFQILSETEGIIPALEPAHAVAQVMKMAPRLKRNSLVVINLSGRGDK
                     DMDIVRERLHLASAA"
     gene            complement(1208..1825)
                     /locus_tag="AC16GM_09970"
     CDS             complement(1208..1825)
                     /locus_tag="AC16GM_09970"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR2924034.1"
                     /translation="MLKVKVCGITNLEDARLAESLGADALGFVFYPKSPRAVTPRLAS
                     TIVKKLSPFTLAVGVFVDAPEKTILLTVRGVGLDMVQLHGSETPQTCRRLARQVPVIK
                     TFRIGGGEFPNVYVYNVDYFLFDTYDSARTGGTGRSFDWKRIAHLKGKADLILSGGLS
                     EDNIAQAVAAVEPAVVDFSSSVEAKPGKKDPEKLRAIFKVINGLR"
     gene            complement(1819..2628)
                     /gene="trpC"
                     /locus_tag="AC16GM_09975"
     CDS             complement(1819..2628)
                     /gene="trpC"
                     /locus_tag="AC16GM_09975"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR2924035.1"
                     /translation="MSILDDIVAVKEREVAEAKTSVPLDCLMKRIQSKVGRPFREALT
                     NGDALSVIAEVKKASPSSGLLQPDYDPVAIAQGYKQNGAAALSVLAEVHYFQGAPEHL
                     QQVSENVPLPVLWKDFVIDEYQIYQAKAAGADCVLLIAALLPGRRLATFLTDVRRLGL
                     EALIEVHAESELQQATDAGADLLGVNNRNLRTLAVDVRTSFRLAERLPQEVVAVSESG
                     IETPETLVELSRLGYRAFLIGTYFLRQANPGRALADLLAKARTLRQKAAVC"
     gene            complement(2628..3974)
                     /locus_tag="AC16GM_09980"
     CDS             complement(2628..3974)
                     /locus_tag="AC16GM_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2924036.1"
                     /translation="MSAAVEQVQEEKLESTPAPEGPPPFFAHPRRWLTDYGTHLALFS
                     RNARLFLFGSFFMGLGFSVFQVLLNLYFKELGFRESTIGDILSVGALGSVLIAGPAAI
                     LLSRRDAKPFLVVTAVLAGLTYSLQATVVQKAGLLAASLGTGMMVSVFRVAAAPFFMR
                     NSTQKERTYLFSVNFGIGTLAGFVGSLLGGWLPAVFSSFDSSIIHSHRWALVSGAGLT
                     LLAAIPFALLRPQAPVSGQSGQKLAWHLLKKKGGLYFKLCFPFLILGLGAGLVIPFLN
                     LYFKTRFQQSSGEIGTFFAILSFATLAGVLTGPVLTRKWGMVRTIVWTQFISIPFMLI
                     LAFTYFLPLAVVAFLFRGALMNLGQPIAVHFTMEQVAPEEHALANSLLMLAWAGAWVV
                     STNLGGKLIARYGFTPPLLVTVGLYIVSSVLYYLYFAHAEKVPKAVEAVPVPEAVG"
     gene            complement(4000..>4207)
                     /gene="trpD"
                     /locus_tag="AC16GM_09985"
     CDS             complement(4000..>4207)
                     /gene="trpD"
                     /locus_tag="AC16GM_09985"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969414.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR2924037.1"
                     /translation="ALILSHLLAGENGPARDVVLLNAGAAIYMAGEAKTIAAGIEVAK
                     KSIDSGAAQKKLERLVAVSQKSVS"
BASE COUNT          933 a         1291 c         1149 g          834 t
ORIGIN      
        1 ggcggcactg gctaaatgta accgctcccg cacaatgtcc atatccttgt caccgcgccc
       61 ggagaggttg ataaccacga gcgaattccg cttcaaccgc ggcgccatct tcataacctg
      121 tgccaccgcg tgggctggtt ccagggcggg aataattcct tcggtctcgg agagtatttg
      181 gaacgcctcc agcgcttcag cgtcggtaac cgccgtgtac tccacccgtc cgctctcttt
      241 caaaaaactg tgctccggtc ccacgccggg gtaatctaaa ccggcagaaa ttgagtgggc
      301 cacctgcacc tgcccgttgc cgttctggag caggtaactc aaactgccgt ggaggatgcc
      361 cgctttaccc cggttcaaag tcgccgcgtg ttttttggta ttcaatccca accccgccgc
      421 ctccacccca accaaccgca ctttggtagt aagaaatggg taaaacatcc ccaacgcatt
      481 ggagccgccg cccacgcagg caaagatgag gtcgggtagt cgccccgcct gaccgatgat
      541 ttgcttttta gtctccctcc caatgaccga ttggaaatcc cggacaatcg tcggatatgg
      601 gtgtgggccg acggtggaac cgagacggta atgagtggtg cgcacgttgg tcacccaatc
      661 gcggatcgct tcgttgatgg catccttcaa agtccgcgag ccggaagtga ccgggatgac
      721 ctgcgctcca agaagtttca ttttgaaaac attgagcgcc tgccgttgca catcctccgc
      781 gcccatataa atggtgcact ccagcccgaa tttggccgcc accgtcgcgg tggccagacc
      841 gtgctgcccc gccccggtct cggcgacgat gcgtgccttg cccagccgct tcgctaacaa
      901 tatctgacca acagtattgt tgatcttgtg cgcgccggtg tgggtcaggt cttcgcgctt
      961 caggtaaatt cgcaggccca gtttgcctga gagacgttcc gcaaagtaga gaggcgtcgg
     1021 acgaccggcg tagtgcgcca gcaaaccccg cagttcgctc tgaaattccc gatcgcgcct
     1081 cgctttccag tagccatccc agagttcgag caaaggcgtt accagggtct ccggcacgaa
     1141 catcccgccg aatttcccga aatgcccgcc tttatcgggc cagcggtagc gctgggattt
     1201 caaagcctta acggaggcca ttgattacct taaagatcgc tcgcaatttt tccgggtctt
     1261 tcttgcccgg cttcgcttca acgctggagg aaaaatcaac caccgcgggc tcgaccgccg
     1321 ccaccgcctg cgcgatattg tcctcactca agccaccgga cagaattaaa tcggctttgc
     1381 ctttcaggtg ggcgatccgc ttccagtcaa aactccttcc cgtgccgccg gtccgcgccg
     1441 aatcgtacgt atcgaacaga aagtaatcga cgttgtaaac gtacacgttg ggaaattcgc
     1501 cgccgcctat ccggaaggtt ttaatcaccg ggacttggcg cgccagccgc cggcaggtct
     1561 gcggcgtctc gctcccgtgc agctgcacca tatccagtcc caccccgcgg acggttaaca
     1621 agatcgtttt ctccggtgca tcgacaaaaa caccgactgc cagcgtgaag ggcgaaagtt
     1681 tcttgacgat tgtactcgcc aaacgcggcg tgaccgctcg tgggcttttc gggtagaaaa
     1741 cgaagcccaa agcatcagcg cccagggact ccgccagccg ggcgtcttcc agattcgtaa
     1801 tgccgcacac tttcactttc agcatacagc tgccttctgc cggagtgtcc tcgctttcgc
     1861 caacaaatcc gccagcgccc ggcccggatt ggcctggcgc aggaagtaag taccgatcaa
     1921 gaatgctctg tagccgagtc gggagagttc caccagcgtt tccggcgtct cgataccgct
     1981 ctcgctcacc gccacgacct cttggggcag tcgttctgcc aggcgaaatg aggttctcac
     2041 atccactgcg agcgtccgca aattgcggtt gttaacgccg agcaaatcgg caccggcatc
     2101 ggtggcttgt tgcaattcag actcggcgtg tacttctatc aatgcctcca gacccaatct
     2161 tcggacatcg gtgaggaagg ttgccaaacg tctccctggg agaagtgcgg caattaagag
     2221 gacgcaatct gcgccagccg ctttggcctg gtaaatctgg tactcgtcga tgacaaaatc
     2281 tttccacaga accgggagcg gaacgttctc gctcacctgc tggaggtgct ccggcgctcc
     2341 ctggaagtag tgtacttcgg ccaaaaccga aagcgccgcc gcgccgttct gtttgtatcc
     2401 ctgagcgatg gccactggat cgtagtcagg ttgcagcagg ccgctggaag gcgacgcttt
     2461 tttcacttca gcgatgaccg agagagcgtc accattcgtc agggcttccc gaaatggccg
     2521 gccaaccttc gattgaatcc gcttcatgag gcaatcgagc ggcacgctgg ttttcgcttc
     2581 cgccacctcg cgctctttga ctgcaacaat gtcatctaaa attgacatta ccccaccgcc
     2641 tcgggtacgg gaacggcttc aacggcttta ggcacctttt ctgcatgggc aaagtagagg
     2701 tagtagagga cagaagaaac gatgtacaga ccgacggtca ccagaagcgg cggggtgaac
     2761 ccgtaccggg caatcaactt gccgccgaga ttggtgctca ccacccaggc cccggcccag
     2821 gcaagcatca aaagactatt ggccagcgca tgctcttccg gggcgacctg ctccatcgta
     2881 aaatgcacgg ctatcggctg gcctaaattc atcaacgcgc cgcggaaaag gaacgccacc
     2941 accgccaaag gcagaaagta ggtgaacgcc agaatcagca taaacgggat ggagatgaac
     3001 tgcgtccaga cgatggtgcg caccatcccc catttgcggg ttagaaccgg gccggtgagc
     3061 acccccgcca gcgtggcgaa agacaggatg gcaaagaagg tgccgatctc gccggaagac
     3121 tgctggaagc gggttttaaa gtagaggttg agaaagggga tcaccaggcc ggcgcccaat
     3181 cccaaaatca aaaacgggaa gcagagcttg aagtagagtc cgcctttctt tttcagcaaa
     3241 tgccaggcca gtttctgtcc ggattggcca ctgactggag cctgcggccg caacagggca
     3301 aagggtatgg ctgccagaag tgtcaaaccc gcgccgctca ccagtgccca gcggtgtgaa
     3361 tgaatgattg aactgtcaaa agagctgaat acggccggca gccagccccc caatagactc
     3421 cccacaaaac cggccagggt cccgatgccg aagttcaccg aaaagaggta agttcgctct
     3481 ttttgcgtgc tgttgcgcat gaaaaaaggc gccgccgcca cccgaaagac cgagaccatc
     3541 atcccggtgc cgagactggc cgccaaaagg cccgcttttt gcaccacggt cgcttgcaaa
     3601 gaataggtga ggccggccaa aaccgcggtg acgaccaaaa acggcttggc gtcacgccgc
     3661 gaaagcagaa ttgccgccgg cccggcaatc aaaaccgaac cgagcgctcc caccgaaagg
     3721 atgtcgccga tggtgctctc ccgaaaaccg agttccttga agtagaggtt caaaagcacc
     3781 tggaagaccg aaaaccccaa ccccatgaaa aatgaaccga agagaaagag ccgcgcgttg
     3841 cgcgagaaga gcgccaggtg cgtcccgtag tcggtcagcc accgccgcgg atgggcgaag
     3901 aaaggcggcg gtccttccgg tgcgggagtg gattccagct tctcttcctg aacttgttct
     3961 acggctgcgc tcacaaattt cctcgccgga ttgatttgat taactcacgc ttttctggct
     4021 gactgctacc agccgttcca atttcttctg cgcggcgccg gagtcaattg attttttcgc
     4081 gacctcaatc cctgcggcaa tcgtctttgc ctctcccgcc atgtaaatgg ccgccccggc
     4141 attcaaaagg accacgtccc gcgccgggcc gttttcaccg gccaggaggt gcgagaggat
     4201 tagcgca
//
LOCUS       DBNGZD010000380         1135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1865384, whole genome shotgun sequence.
ACCESSION   DBNGZD010000380 DBNGZD010000000
VERSION     DBNGZD010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1865384"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16GM_09990"
     CDS             <1..354
                     /locus_tag="AC16GM_09990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2924038.1"
                     /translation="VDQPMTSVFLLKRRTMNQTGLFDESFPIFFNDVDYCRRIWIAGF
                     SIAYVPQAQWYHRLGGATGQRKFRMTFVSHRSFFRYFWKHASLIGRLGLVLLWPFFWL
                     LLFPRLLAAVFVQPD"
     gene            complement(351..>1135)
                     /locus_tag="AC16GM_09995"
     CDS             complement(351..>1135)
                     /locus_tag="AC16GM_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2924039.1"
                     /translation="FIGHSRWGWIFSSYLLMQKFVESIFTNPQGYHLGVAFALGKISF
                     PAYRDLNYNQLLFGDPEMPIYTAPPRPIALSAPASLPIGYSSFVVNVTADNQPLSQAT
                     VVITNRAESILTVAQTDANGNAAFSLSLPTVEPLTVTATRPNYLPVEKTIQVGGTPVT
                     DNDKFKGVLPTLLGQNAPNPFNPLTVITYQLAKSEPARLEIYNLLGQKVRTLVDVQEK
                     AGAHREAWDGRDEKGLEVASGIYFYRLSTPSFSAARKMTLLR"
BASE COUNT          234 a          288 c          331 g          282 t
ORIGIN      
        1 gtggatcagc cgatgacttc ggtctttttg ctcaaacgga ggacgatgaa ccaaacgggg
       61 ttattcgatg aatcatttcc gatttttttc aatgacgtcg attattgccg ccgcatctgg
      121 atcgctggat tttccattgc ttacgttccg caggcgcagt ggtaccaccg gttggggggt
      181 gcgacgggtc agaggaaatt caggatgacg tttgtttccc accggagttt tttccgctac
      241 ttctggaaac atgcctcctt gatcggtcgg ctggggctgg tactcctctg gcctttcttt
      301 tggctcttgc tttttccgcg actcctggca gcggttttcg ttcaaccgga ctaacgcagc
      361 aaggtcattt tgcgcgccgc actgaaggag ggggtgctta agcggtagaa gtaaatgccg
      421 ctcgcaacct caaggccttt ttcatctctg ccgtcccacg cctcgcgatg ggcgccggct
      481 ttctcttgca cgtccacaag tgtccgcacc ttttgaccca gaagattgta aatctccagc
      541 cgggccggct cacttttcgc caattggtag gtgatcaccg ttagcggatt gaacggattg
      601 ggagcgttct ggccgagcag cgtaggtagt acccctttga atttgtcgtt gtcggtgacc
      661 ggcgttccac cgacttgaat ggttttctca accggcaggt aattcggtct ggtagcggtc
      721 accgtaagcg gttcgaccgt cggcagagag agcgaaaagg cggcgtttcc attcgcatcg
      781 gtctgggcca ccgtcaaaat tgattcggcc cggttcgtta tgacgacggt tgcctgggaa
      841 agcggttggt tatcagcagt tacgttgacc acaaaagaag agtatccgat gggtaaagac
      901 gcaggcgcag aaagggctat gggccgcggc ggcgcggtgt agatcggcat ttccggatcg
      961 ccaaatagaa gctggttgta atttaagtcg cggtaggcgg gaaaacttat tttgcccagc
     1021 gcaaaggcca cgcccaaatg atagccctgc ggattggtga agatcgactc cacgaacttc
     1081 tgcatcagga ggtacgaaga aaaaatccag ccccagcgcg agtggccgat gaacg
//
LOCUS       DBNGZD010000381         8447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1449817, whole genome shotgun sequence.
ACCESSION   DBNGZD010000381 DBNGZD010000000
VERSION     DBNGZD010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1449817"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="AC16GM_10000"
     CDS             <1..1191
                     /locus_tag="AC16GM_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2924040.1"
                     /translation="NPFYAKTGLAREYWQSGLEYVALFLRHYALYGLVLVFPLFGWKK
                     LTVFSRFCLFVFSGYGLYVIAVGGDVLKAHRFFVPVLFFGYFPFADALYQVMRNWRYK
                     GLVFAALVLVFGYSTYRIPLLYLQTSALTERILLADNTRTTRFFAPDTTAKSFAVSAL
                     GVYSYYLGDRRFIDMLGLTEPVVAKHPEKIAGLVSTWRERHFNAGHVLSQKPDVILFS
                     TGMKPASLAERALFLYPDFRRNYRLEFLSQEGFFNNYYRRFRDLPCDPKPDQPAQFVN
                     LFNEGCNWIARDRRRAFALFQEALARGPKDFPVLYTSLGYLFHAFGMLDSAEAYLRKG
                     MEMDGCGSMTRYYYSNLLYTQKRYAEAAAQDSILVRTVPNALDFLRTTRGMGAAAQRR
                     FDNR"
     gene            1218..1294
                     /locus_tag="AC16GM_10005"
     tRNA            1218..1294
                     /locus_tag="AC16GM_10005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1252..1254,aa:Gly,seq:gcc)
     gene            1392..1463
                     /locus_tag="AC16GM_10010"
     tRNA            1392..1463
                     /locus_tag="AC16GM_10010"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1424..1426,aa:Cys,seq:gca)
     gene            1559..2179
                     /locus_tag="AC16GM_10015"
     CDS             1559..2179
                     /locus_tag="AC16GM_10015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012943558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR2924041.1"
                     /translation="MEDRVSEEVAARRKAAYPIHPLILNRWSPRAMSGDELTDEELFP
                     LFEAARWAPSSYNNQPWRIIIARTPADRERFLEFLAPGNRTWCIRAAVLVVVISRNTF
                     EYNSKPARTHSFDTGAAWENLALEGSRRGLVVHGMQGFDYEKAKQVLGVPDDYTVEAM
                     IAIGKLGDKSLLPEKLQQVEVPSDRKPLAEIVFEGCFGSKLADTKS"
     gene            2334..3095
                     /locus_tag="AC16GM_10020"
     CDS             2334..3095
                     /locus_tag="AC16GM_10020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2924042.1"
                     /translation="MYGRKSKSKEEGVTPFFTEGIHYGKGGINNNWVTPLQHVPAGKG
                     LILDLGCGGGETRNVLETKGYTWVGLDIQKMKGVLVVGDAHQLPMKDEAVTAVYSCQV
                     FEHLVQPWRAAGEVFRVLKPGGIFYGSMSCLEPFHHSYFNYTHWGVEALLRHAGLVPR
                     RIEAGSSAFLILLHHLVDGAGPELSTPLAKLTVKPFVWFLRFFGKMFIMLRYGRTSSQ
                     MRKIDGYFAKFALRFAGHIQFVAEKPEPLSETGDF"
     gene            complement(3341..3928)
                     /locus_tag="AC16GM_10025"
     CDS             complement(3341..3928)
                     /locus_tag="AC16GM_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924043.1"
                     /translation="MKTTQIKQDTAAHPLRLWRKLGLASLALAVTFLASACSERGNKD
                     EARKEASVKSAEAAEPMTPNAAAEENDSLGLPRAQEQEGTVVTADSLLPDVSAFAADT
                     LTYPGGTVEITARTSSDVTAVALWDGIGQKQAFTCDSEAKVWRITYRVPLKPLWERLG
                     LSVTARNSAKKWRRVWVFLQLQGQAPQAQAAPVEK"
     gene            complement(4003..4596)
                     /locus_tag="AC16GM_10030"
     CDS             complement(4003..4596)
                     /locus_tag="AC16GM_10030"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR2924044.1"
                     /translation="MSKRKNLAIAIAISLSASAVNARPGDSLHIVINIPAFRLFLFEG
                     DSLLTSYSIAVGKPETQTPVGTFTVKRKVMNPSYWPKGQPPVPPGPGNPLGRYWIGLS
                     QRDLGIHGTNEPHSIGTAASGGCIRMRESDLLALYDRVLLGTRVEIVYETRREGGGRQ
                     SAYADIYHHNLNNSKRNTTEPEPETLPLLAQGNSGKR"
     gene            4873..6192
                     /locus_tag="AC16GM_10035"
     CDS             4873..6192
                     /locus_tag="AC16GM_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924045.1"
                     /translation="MVSIQKKIWWLSSAFLFSLISHHEVCAEALRLNAKVIWTQADRV
                     YCVSSDSGAFEPAKNLTFVYRGREVARGKIVALYNRELALVQLQWGSLINIDHLDSVE
                     VAEQPAPLPPLSRLRIGYPSQKRSNLIFSCSEMAFDHSSIPAAFRPDTLEDAGDQWVR
                     DSLKNPKPDPWPDTLFVREVDEVSDEEIALERGELDIAVFWPGELSQHMRKDPRWQNP
                     AFGRAQQGLVAALWLSQKIDEDDSNLCDLRRWYDFDLFNEELFRGDLVPVEQAAAWAA
                     QSPAVQPHPPAPVVRFQVERSWPGQRLVERFLNRRSKSSPAVTTPGQMETVRLFYLDA
                     PVSSFDSISQALASYLQGGDFPQAIRAQADTLLTLVKAAHDAPDSVRVSALDTVRHYM
                     RSRLHVTFLFALRCPVISQPELRPYLELLRVDALVNLVHCQQSDRKQ"
     gene            6189..7916
                     /locus_tag="AC16GM_10040"
     CDS             6189..7916
                     /locus_tag="AC16GM_10040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2924046.1"
                     /translation="MNSLRFRLILGFSFIAIVPLAVAIYFLSQRVEKTVAELAAERLA
                     AALGTLQSQLRFDGERVAGKLQILGKDPLLKRLYLLRPPGSAGTRDLSDYLAEKRFLL
                     GLDFLQVVDTGGLVTSDGSLSSATLEKTYRDSLHARARAGITSSGLVIDTVDTISVLT
                     LTATAPVRYENRSVGVVRGGLVFDAASLARLKQTSGVDLLLRDSSGRTIAATIFPAAE
                     AVSPGFEGGGRVTLAGRSYLAQTIPLETSGPPLAVITGLVSTGPADQTIASLQVTSTL
                     LGLFGLGIAIFLGIVWSRQISGPVERIAQFSQKIAQGQWDEPLTLESVRELEMLVAAL
                     NRMRDDLRTYRDKLVTSERQAAWSVMARKVAHEVKNPLTPIAISIADLQRSFEQKRPD
                     FPQILAQSAKTVTEEIETLKRLLQEFSDFAKFPPPRFAPCLLSELFTDVKTLYARDVD
                     EGRLTFTEEVREITLSADAGQIKQALINLIKNGLEAVDGQGQVRVAVNVTDSALEIDV
                     ADNGPGLTAEQKANLFTPYFTTKAQGSGLGLTIVQRIASDHGGQITVDSLPGRGTTFR
                     LLLPLELKR"
     gene            7919..>8447
                     /locus_tag="AC16GM_10045"
     CDS             7919..>8447
                     /locus_tag="AC16GM_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2924047.1"
                     /translation="MPSILVVDDEKNILSSLRGALGREGYQVDCAATLAEARVKLREA
                     YDFILLDVWFPEGSGLDLLEEITAGAPETVVVMMSGHGTIETAVKATKLGAYDFLEKP
                     ISLDRLLVLLRNAATTLTLRALNRRLQAERPWAVAIVGRSPAVQKLLREIEQAGPTAA
                     RVLIQGEHGTGKELVA"
BASE COUNT         1694 a         2337 c         2484 g         1932 t
ORIGIN      
        1 aatccctttt acgccaaaac cggcctggcg agagaatact ggcaaagcgg cctggagtac
       61 gttgccctct tcctccggca ttacgccctg tacggtctag tcttggtatt cccgctcttc
      121 gggtggaaga aattgacggt tttttcccgt ttctgtcttt tcgtcttttc cggctacggg
      181 ctgtacgtca tcgccgtggg cggggatgtt ttgaaagcgc accgtttctt tgtccccgtg
      241 cttttcttcg gctacttccc gtttgccgat gcgctgtacc aggtgatgcg caactggcgg
      301 tataaagggt tggtctttgc cgcactggtt ttggtcttcg gatactctac ctaccgcatt
      361 ccgcttctct acctgcaaac gtcagcgctc acggagagaa tccttttggc ggacaacacc
      421 cggaccaccc gcttctttgc ccctgatacc acggcaaaaa gctttgccgt ctccgctctc
      481 ggcgtctatt cctactacct cggagaccgc cggttcatcg acatgcttgg cctgaccgaa
      541 ccggtggtgg ccaaacatcc cgaaaagatc gcgggattgg tttcaacctg gcgggagcgg
      601 cacttcaacg ccggccatgt gctttcgcag aagccggacg tcattttgtt ttccacgggc
      661 atgaagcccg cctcgctggc cgagcgggcc ctgtttctct atccggactt tcgccggaac
      721 taccgactgg agtttctgtc tcaggaaggt ttttttaaca attactaccg gaggtttcgg
      781 gacctgccct gcgacccgaa gccggaccag ccggcgcaat ttgtcaatct tttcaacgaa
      841 ggatgcaact ggattgcccg ggacagacgc cgggcctttg cgctcttcca ggaggccctg
      901 gcccggggac caaaggactt cccggtgctg tatacctccc tggggtatct ctttcacgcc
      961 ttcgggatgc tggattcggc ggaggcctac ctgcgcaaag gaatggagat ggacggctgc
     1021 ggctccatga cccggtacta ttacagcaat ctgctctaca cccaaaaacg ctacgccgaa
     1081 gcggccgcac aggattcgat tctggtgcga accgtcccca atgccctcga ttttctgcga
     1141 actacccgag ggatgggggc tgcggcgcag cgccgatttg acaaccgttg aggaagggtt
     1201 tatttttttt ctccagcgcg ggaatagctc agctggctag agcgtcacct tgccaaggtg
     1261 aatgtcgcgg gttcgaatcc cgtttcccgc tccagacaaa gcagtgataa agtgatgagt
     1321 gatggagtga tgaagtgagc gggtgcatcg tgcatcactt catcacttta tcacttgtca
     1381 ctgcagtttt cggcggctta gccaagtggt aaggcagagg tctgcaaaac ctctattctc
     1441 cggttcgaat ccggaagccg cctcaataag ggcagtgatt agggatcagg gattagtgat
     1501 taaaaacgct caataaaaga tgcggagata aagtctaacg ggaaatttag aggttgagat
     1561 ggaggaccga gtctcggaag aggtcgcggc cagacggaaa gcggcgtatc ccattcaccc
     1621 gctgatcctc aaccgctggt cgccgcgggc gatgagcggt gatgaattga ccgacgagga
     1681 gctgttcccc ctgttcgaag ccgcgcgctg ggcgccctcc tcctacaaca atcaaccctg
     1741 gcgcatcatc atcgcacgaa cgccggcgga ccgggaaagg tttctcgaat tcctcgcacc
     1801 ggggaatcgg acctggtgca tccgggcggc ggtgctggtc gtggttatct cccggaatac
     1861 atttgagtac aatagtaagc cggcgcggac ccactctttt gacaccggcg cggcatggga
     1921 aaatttagcc ctggaaggga gccggcgcgg gctggtcgtg cacggaatgc aggggttcga
     1981 ctacgagaag gcgaagcagg tcttgggtgt gccggacgat tacaccgttg aggcgatgat
     2041 cgccatcggc aagctgggcg acaaatccct tttgccggaa aagttgcagc aggtggaggt
     2101 cccctctgac cgcaagccgc tggctgaaat tgtttttgaa ggttgctttg gatctaaact
     2161 ggcagacact aaatcctaaa cacctatctt ttcatcttct gttgtcgcgg catggggtgt
     2221 tggtgttgct aacgggtaca agccggcgga gtgggcgatc tgtagcttaa ggggtcgatc
     2281 ccccgggacg cagttttagg aattcagcaa tagggtagct ttgtgctttc ctaatgtacg
     2341 gccgtaaatc caaatcaaaa gaagagggag taaccccatt ctttaccgaa ggaattcatt
     2401 atggtaaagg gggaattaac aacaactggg tgacgccact gcaacacgtg ccagcgggaa
     2461 agggattaat ccttgatttg ggatgtggag ggggtgagac ccggaatgta ttggaaacca
     2521 agggatatac ctgggtaggg ttggatatcc aaaaaatgaa aggcgtgttg gtcgttggag
     2581 atgctcacca acttccaatg aaagacgaag cagtcactgc ggtttatagc tgtcaggtgt
     2641 ttgaacattt agtgcagccg tggcgggcgg ccggtgaggt gtttcgggtg ctgaaaccgg
     2701 gaggaatatt ctatggcagc atgtcttgcc tggagccctt tcaccattcg tatttcaatt
     2761 acacccattg gggagtagag gcgctgttgc gccacgcggg attggttccc aggcggattg
     2821 aagccgggtc cagcgctttt ttgatcctgc tccaccattt agtggatgga gccggcccag
     2881 agctatcgac cccgctggcc aagttaacgg tgaaaccatt cgtgtggttc ttgcggtttt
     2941 ttggcaaaat gttcatcatg ctgcgctacg gacgaacgtc ctcccagatg aggaaaattg
     3001 acggctattt cgccaagttt gcgctgcgtt ttgccggaca cattcagttt gtagctgaaa
     3061 aaccggagcc gttgtcggag acgggtgact tctaaacttc gacagtggcc aaaccatata
     3121 gagtgggcac ctctgagtgc tctccccagg cactcttccc ttcagggtca aatgggtaaa
     3181 aggatcagcg cctaccaccc aacgtctttt cgtgatcaat tgcagtttga ttggtaaccc
     3241 cggtttttcc tggatgataa aaaacgccgc agatcacctg ctcacgggag ctttgacctg
     3301 cggcgctttc gtcttaccac ttaactacag ggctcggcgg ttacttttcc accggcgcgg
     3361 cttgggcttg cggcgcttgc ccttgaagct gcaggaagac ccagacccgg cgccactttt
     3421 tggcgctgtt cctggcggtc accgacagcc ccaggcgctc ccagagcggt ttcaggggca
     3481 cccggtaggt gatccgccag accttcgcct cggaatcgca ggtgaaggcc tgtttttgcc
     3541 cgatgccatc ccagagggcg accgcggtca cgtcggagga agtccgggcg gtaatttcca
     3601 ccgtgccgcc ggggtacgtc agcgtgtcgg cggcgaaagc cgaaacgtcg ggcaggagag
     3661 aatcggcggt taccacagtt ccttcttgct cctgggcccg cggcaaaccg agcgagtcgt
     3721 tctcttctgc ggcggcgttc ggggtcatcg gttccgccgc ttctgccgat tttacacttg
     3781 cttctttccg ggcctcgtcc ttgttccctc gctcactgca ggcggaagcg aggaaagtca
     3841 cggccagagc gagcgaggcc agccccagct tgcgccagag gcgcagcggg tgagcggcgg
     3901 tgtcctgctt gatttgggtg gttttcatct gtctctcctt tccggttttt gatgattttt
     3961 tactgattga ccgtcaggta ctggttactg gcaatcgatc agttaacgct tccctgaatt
     4021 tccctgtgcc aagagcggca gagtctcagg ttcaggctcg gtagtgttgc gtttgctatt
     4081 gtttaggttg tggtggtaga tgtccgcata cgcgctttgc cggccgcccc cttctcgccg
     4141 ggtttcatag acgatctcca cccgggtgcc aaggagaacc cggtcgtaca atgccaggag
     4201 gtcgctttcc cgcatccgta tgcaaccgcc ggaggcggcg gtgccgatgg agtgcggttc
     4261 gttcgtcccg tgaattccca ggtcgcgttg cgagaggccg atccagtacc ggccgagagg
     4321 attacccggc cccggaggaa ccggcggctg gcccttgggc cagtaactgg gattcatcac
     4381 cttgcgcttt acggtgaacg tgccgacagg cgtctgcgtc tccggtttgc ccacggcgat
     4441 cgagtatgaa gtcaaaagac tatcgccctc gaaaaggaag aggcgaaaag caggaatatt
     4501 gatgacgatg tgcagtgaat cgccgggtcg agcgtttacg gcagaggccg ataaactgat
     4561 tgcgatggcg attgcgaggt tttttcgttt tgacatcatc atcttcatct ccgggagcaa
     4621 atgatgtcaa aaccaatgcc gtctacttat ttggttgtcg tttaacaggt tatgctgatg
     4681 aggcggtcaa aaagagaaga aaatatgtgt caaaacgaac acgcacatgg tcttaactgt
     4741 taggatggta acggaaagtt taatgactac aattagttag tcgttcgtgg caaatttgac
     4801 acgtataagt atttttggga cacagaatag ggaactttga agttcgagag cagtaaggat
     4861 tatgttgagg gtatggtctc aattcagaaa aaaatttggt ggttaagttc ggcctttctt
     4921 ttttcgctta tttcccacca cgaggtttgt gcggaagcct tgcggctgaa tgccaaagtg
     4981 atttggacgc aggccgaccg cgtctactgt gtttcctccg attccggtgc cttcgagcca
     5041 gccaaaaatc ttacgttcgt gtaccgcggc agggaagttg cccgggggaa aatcgtggcg
     5101 ttgtacaacc gcgaactggc gctggtccaa ctccaatggg gctcgttaat taatattgac
     5161 cacctggatt cagtggaggt cgcagagcag ccggcacccc tcccgccttt atcccgcctg
     5221 cggatcgggt atccttctca aaaacggtct aatctgattt tttcttgttc tgagatggca
     5281 tttgatcatt caagcattcc cgctgccttc cgtccggaca cgctggagga tgcaggcgac
     5341 caatgggtgc gcgattcgct gaaaaatcca aagcccgatc cctggccgga tacccttttc
     5401 gtccgggagg ttgacgaagt ctctgatgag gaaattgccc tggagcgggg tgaactggat
     5461 atcgccgtct tctggccggg cgaactctcg cagcatatgc ggaaagatcc ccgctggcag
     5521 aaccccgcgt ttgggcgcgc gcagcagggg ctggtcgccg ccctctggct gagtcagaaa
     5581 attgatgaag acgactccaa tctctgcgac ctgcgacgct ggtacgattt tgaccttttc
     5641 aacgaggaac tgtttcgggg cgacctcgtg cccgttgaac aggcggccgc ctgggcggct
     5701 cagtctcccg cggtacagcc gcacccaccg gccccggttg tgcgctttca agtcgagcgc
     5761 agttggccgg gacagcggct ggtggagcgc tttctgaacc gtcggagcaa gtcgagccct
     5821 gccgtgacta ctcccggtca gatggaaacg gtgcgcctct tttatctcga cgctccggtt
     5881 tcttctttcg attccatttc tcaggcactg gcgagctacc ttcagggcgg cgactttccc
     5941 caagcaatcc gcgcccaggc ggacacactg ctgacgctgg ttaaggccgc ccacgacgct
     6001 cccgatagcg tcagagtgtc ggcgctggat acggttcggc actacatgcg cagtcggttg
     6061 cacgttacct ttctctttgc cctacgctgc ccggtcatca gtcagccgga actccgtccc
     6121 tatctggagt tattaagggt tgacgctctc gtgaacttgg ttcactgtca gcaatctgat
     6181 cggaaacaat gaactccctc cgcttccgct tgattctggg tttctccttc attgccatcg
     6241 tccctttggc ggtggcgatc tactttttgt cccagcgggt ggaaaaaacg gtggccgagc
     6301 tggcggctga gcgtttggcg gcggccctgg ggacgttgca gagccagttg cgtttcgacg
     6361 gtgaacgagt tgccggaaaa ttacagattc tgggaaaaga cccgctcttg aaacggctat
     6421 accttttgcg gcctcccggc agcgccggta cgcgggatct gtccgactat cttgcggaaa
     6481 agcgtttcct actggggctg gatttcctgc aggtagttga caccggcggc ctggtgacga
     6541 gcgacggatc gctctcctca gccacgctgg aaaaaaccta ccgcgattcg ttgcacgcgc
     6601 gggcgcgggc ggggatcacc tcctccggtc tcgtcatcga tacggtcgat accatctccg
     6661 tcttgacttt gaccgccacc gcgcctgtgc ggtacgagaa caggagcgtc ggcgtcgtgc
     6721 gcggcgggct ggtctttgac gcggcatccc tggcgcggtt gaagcagacc agcggcgtcg
     6781 atttactgct gcgggacagt tccggccgca ccattgcagc aaccattttc ccggccgccg
     6841 aggccgtctc gcccggcttt gagggagggg ggcgcgtcac cctcgcgggg cgttcctatc
     6901 tggctcaaac cattcccctg gaaacttccg ggccgccact ggccgtcatt accggcctgg
     6961 tttcgaccgg gccggcggat cagaccatcg ccagtctgca ggtaacgtcg accctcctgg
     7021 gacttttcgg actggggatc gccattttcc tggggattgt ctggtcgcgg caaatatccg
     7081 ggcccgtgga gcggatagca cagttttccc agaagattgc ccagggacaa tgggatgagc
     7141 cgctgacgct ggagagcgtc cgtgaattag agatgctggt cgccgcctta aaccggatgc
     7201 gggacgacct gcgcacctac cgcgacaagc tggtgacgag cgaacgccag gccgcctgga
     7261 gtgtgatggc ccgcaaggtg gcccacgaag tgaaaaaccc tttgacgccg attgccatat
     7321 cgatagccga tctccagcgc agttttgaac aaaagcgccc cgattttcct caaattctgg
     7381 cgcagtcggc taaaaccgtg acggaagaga tagaaacgct caagcgcctg ctgcaggagt
     7441 tctccgactt cgccaagttt cccccgcccc gtttcgcccc ctgcctcctc tccgagcttt
     7501 ttaccgatgt caagacgctc tatgcccggg acgtggacga ggggcggctg acttttaccg
     7561 aagaggtgcg agagattacg ttgagcgcgg acgccggaca gatcaagcag gcgctcatca
     7621 acttaatcaa aaatggctta gaagcggtgg acggccaggg ccaggtacga gttgcggtga
     7681 acgtaaccga ctcagcgctc gaaatcgacg tggccgacaa cggcccgggt ttgaccgcag
     7741 agcaaaaagc caacctcttc accccgtact ttaccaccaa agcgcagggg agcggcctgg
     7801 gcctgaccat tgtccaaagg atcgcaagcg accatggggg acagatcacc gtcgattctc
     7861 taccggggcg cggcaccacc ttccgcctgc ttttgcctct ggagttgaaa aggtagcgat
     7921 gccttcaatc ttagtcgttg acgacgaaaa aaatattctc tccagcctgc ggggggccct
     7981 gggacgcgag gggtatcagg tggactgtgc ggcgaccctg gccgaggccc gggtaaaact
     8041 ccgggaagcc tacgacttca ttctgctcga tgtctggttt cccgagggca gcggcctcga
     8101 tctgttggaa gaaattaccg ccggtgcgcc ggagacggtg gtcgtcatga tgtccggaca
     8161 cgggaccatt gaaacggcgg tgaaagccac caaattaggg gcttacgatt ttctggagaa
     8221 gccgatttcg ctcgaccgat tactcgtcct cttacggaac gcagccacga cgctcaccct
     8281 gcgggcccta aatcgccgtc tgcaagcgga acgcccctgg gcggtggcaa tcgtcggccg
     8341 ctccccggcg gtgcagaaac ttttgcgcga gatcgagcag gccggtccca ctgccgcccg
     8401 ggttctaatc caaggtgagc atgggacagg caaagaatta gtggccc
//
LOCUS       DBNGZD010000382         2279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_893658, whole genome shotgun sequence.
ACCESSION   DBNGZD010000382 DBNGZD010000000
VERSION     DBNGZD010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_893658"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..>2279
                     /locus_tag="AC16GM_10050"
     CDS             301..>2279
                     /locus_tag="AC16GM_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924048.1"
                     /translation="MRRRYKIPLFLLAALGLFILGAYLVLTRTSLLKSQINRYLAGYL
                     SSQFPLEVKVGKVGGVPWKSIVLEDVTVDYTVKPHAYRMLTIDRIEAHYRLSNVWNQE
                     WIASRLDLVGVKAVLKQDENGDILFPQYLGNQKKGKAELNFQVDTLRVADGSFEFFTP
                     QRSYKADSLTLLGRCSGSGTTIDFAVSDLRFALPQERIRTQKALFAGQYREGRLWLDS
                     ALIQTDSSLLAFGGTLVLAERPQLSAKVERGRIDFGEFKRLTGFDLQGDVAFSGRVKS
                     DFHETRGQTKLSGIFFGHTLNNLAVDYLYQQKTLFCSALSGEAAGTQLSGRGKFDFTG
                     KPERYEFSGRVNHLDLRRWVSTDLASDFNGEVQLVGAGLSDKTLRLNLTVALDSGRLD
                     RYRFHQVRGALTLDAHRATFADDFTVRYKNAAFTFAGSVGFTDSLNLHGTIDLPDLSD
                     FRGQTFIKEPAGRGEATFLATGRTLDPDIQGTFWSDSCWLYQLFSSRLEGVYAVRRFE
                     TARQGRIRLSLANGTAWDVPYDSLSAVFTLDSTRAYFDSARFWRADLYVQGDGVIDYA
                     DSLVTKLTVQGLSGDYRGNAFGLGRPARISVDTAGYDLNDVLLENGSGTVAVKGRIDY
                     SEKMDLDLTGQNAALGPWTRFFFRKKEEVGGLLAFS"
BASE COUNT          481 a          643 c          659 g          496 t
ORIGIN      
        1 cgccctggga aatttacgga attacagttt caaccgggaa gaagagacgt tatccaaagc
       61 agaaaaagaa agcctccaga agcaactgga aaaactccag gagcagatgg aaaaactcca
      121 ggaggaactc tcccgcctgc aggaacagaa aggcaaggag tagccgtttc tcgccggctg
      181 aaaaagaata accgcagcgc cccgtccccg cggggcgctt tgctttccgc gtcgcgggga
      241 aagattgacg ttgattttac ctcgccagtt tccgattttt atccgtaggg aatactaggg
      301 atgcgccggc gctacaagat acctctgttc ctcctggcgg cactggggct cttcattctg
      361 ggagcttacc tggtgctgac gcggacctcc ctgctcaaaa gccagatcaa ccgctacctg
      421 gccggctacc tctccagcca gttcccgctg gaggtgaaag tggggaaggt ggggggagtg
      481 ccgtggaaat cgatcgtgtt ggaagacgtg acggtcgatt acacagtcaa gccccacgcc
      541 taccggatgc tcaccataga ccggattgaa gcgcactacc ggctctccaa cgtctggaac
      601 caggagtgga ttgccagccg cctcgattta gtcggcgtca aagcggtgct caagcaggac
      661 gaaaatggag acatcctgtt tccccagtat ctcgggaatc aaaaaaaggg gaaggccgag
      721 ctcaactttc aagtcgatac gctaagggtt gcggacggtt cgtttgagtt tttcacccct
      781 cagcgttcct acaaggcgga cagcctgacc cttttggggc gctgcagcgg cagcggcacg
      841 accattgatt tcgccgtttc cgacttgcgc tttgccctgc cgcaggaaag aatccgcacc
      901 caaaaggcgc tctttgccgg tcagtaccgg gaaggtcgcc tctggttgga ttcagcttta
      961 atccagaccg attccagtct gctggccttc ggcgggacac tggttttggc cgaacggccc
     1021 cagctctccg ccaaggtcga gcggggccgc attgacttcg gagaattcaa gcgtctgacc
     1081 ggttttgatc tgcaagggga cgtggccttc agcggccggg taaaatcgga tttccacgaa
     1141 actcgtggcc agaccaaact ctcgggcatt ttcttcgggc atacgttgaa taatttggcc
     1201 gtcgactatc tctatcagca aaaaacgctt ttctgttcag cactttccgg cgaggcggcc
     1261 gggacgcagc tgtcgggccg ggggaaattc gatttcaccg gcaaaccgga acggtacgag
     1321 tttagcggga gggtgaacca cctcgatctc cgccgctggg tctcaaccga cctcgccagc
     1381 gatttcaacg gggaagtaca gctcgtcggt gcggggctgt cggataagac gctccggctc
     1441 aacctgacgg tggcgctcga ctccggacgc ctggataggt accggtttca ccaagtccgc
     1501 ggagcactta ccctggatgc ccaccgggcg actttcgccg atgatttcac cgtccgctac
     1561 aaaaatgcgg catttacctt tgccggatcg gtggggttca ccgattcgct caatctacac
     1621 ggcactatcg acctacccga tctttccgat ttccggggtc agaccttcat taaagaaccg
     1681 gccggccggg gagaggcgac ttttcttgcc accggcagga cgcttgatcc tgatattcag
     1741 ggcactttct ggtccgactc ctgctggctg tatcagctct ttagttcccg cctggaaggc
     1801 gtgtatgccg ttcggcggtt tgagacggcg cggcagggga gaatccgcct ttctctggcg
     1861 aacggaaccg cctgggacgt gccgtacgat tcgctgtcgg ctgttttcac gctggatagt
     1921 acccgagcgt actttgattc ggcgcggttc tggcgcgccg acctctacgt ccaaggtgac
     1981 ggggtcatcg actacgccga ttcactggtc accaaactga cggttcaagg tctctccggc
     2041 gattaccgcg ggaatgcctt tggcctcggc agacccgccc gcatctctgt tgatacggcg
     2101 ggttatgacc tgaatgacgt gctgcttgaa aacggttccg gaacggtggc ggtaaaaggg
     2161 cgcattgatt acagcgagaa aatggattta gatctcaccg gccagaacgc tgctctcggt
     2221 ccctggacaa gattcttctt tcggaagaaa gaagaagtag gaggactttt ggctttctc
//
LOCUS       DBNGZD010000383         3738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_683475, whole genome shotgun sequence.
ACCESSION   DBNGZD010000383 DBNGZD010000000
VERSION     DBNGZD010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_683475"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="AC16GM_10055"
     CDS             <1..1491
                     /locus_tag="AC16GM_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR2924049.1"
                     /translation="VSTITFLVSLFLFFRFDPQNPGFQFSIDVPWIPSFGIHYHLGLD
                     GISLFLVLLTTFLTPIAIWSSWTAITEKLKGYLISILILESAMSGVFLALDLFLFFVF
                     WEAILIPMYFLIGIWGGPRRIYATIKFVLFTMTGSRLMFVAIIVLFFLYHSHTGQYSF
                     NLLDYYKLPIPLGAQTWLFLAFALAFAIKVPMFPFHTWLPNAHVEAPTAGSVILAGIL
                     LKMGTYGFLRFCWPLFPNAFEQFLPMMIVLSLIGIIYGAWMSMVQKDIKSLIAYSSVS
                     HLGFVMLGLAVLNLQGLQGSLLQMLNHGISTGALFLIVGMIYERRHTRLIADYGGITK
                     VVPVCATFFLIVALSSIGLPGTNGFVGEFLILLGDFKFNWIYAALGATGVIFAAVYML
                     WMYQRVMFGPVTNPANSALTDLNRRERWTLIPLVVLIFWIGLYPKPFLSRMEGSLANL
                     VTQVEERRGYLSQYRNGEIVSPVPQGLLTSTPQHPDTSTLNQASRP"
     gene            1488..2966
                     /locus_tag="AC16GM_10060"
     CDS             1488..2966
                     /locus_tag="AC16GM_10060"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR2924050.1"
                     /translation="MTITPPDLVFSGLVPEIVVVGFATLLMVVGPFFKQRGQGVVFWL
                     TLIGLALAAFTSILLWNQTISAMGGMAYSDNFATFFRLVFLLAAFVVVLFSKAYLKWE
                     NLPAAEYYSLLLFSTFGMMLMAGSLNLIVIFLGLEVMSIPLYILAGYRRSDFRSIESS
                     MKYFLIGAFASGFLLYGIALIYGAVGSTDLEKIAQAISSGQVATDFLFLSGVGLLLVG
                     FGFKVASVPFHMWTPDVYEGAPTAVTGFMAAGPKAAGFAALLRVFSIFLAGLNHNLMP
                     AFWILSVLTMTTGNVIALSQPNLKRMLAYSSIAHAGYILVALVAGGEAGIASALFYLL
                     AYSLMNLGAFACVCAVEKVTGRGNEIASYAGLASRHPLVAIALTIFLVSLGGLPPTAG
                     FVGKFYVFSAAVNAGQIPLVIIAVLNSLISVYYYLRPVVVMFMQAPEAVPASAPEGSA
                     TAFAWPVSLALSIFITAWGTLQLGIFPQALLELARQTVSAFL"
     gene            3017..3457
                     /locus_tag="AC16GM_10065"
     CDS             3017..3457
                     /locus_tag="AC16GM_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR2924051.1"
                     /translation="MRDLVKNRLQLLSQRVKKRRLRLTRQRTIVLEELAKVDTHPRAD
                     QLYRMVRQRLPTISFGTVYRNLKTLCQLELVTELKLGQDSSRFDARTDPHHHVACTHC
                     GCVVDVELPVESGLQKKAARQTGFAVSHYQTAFFGTCPRCRGKS"
BASE COUNT          626 a         1096 c         1017 g          999 t
ORIGIN      
        1 gtttcgacca ttaccttcct ggtctcgctt ttccttttct tccgattcga tccacagaat
       61 cccggatttc agttttcgat tgacgtgccc tggattccgt ccttcggcat ccactaccac
      121 ctgggactcg atggcatcag ccttttttta gtcctcctca ctacattttt gacgcccatt
      181 gccatctggt cgtcctggac ggccatcacg gaaaaactca aggggtactt gatttcaata
      241 ctcatcctgg aatcggcgat gagcggcgtc tttctggcgc tggatttatt cttattcttc
      301 gtcttctggg aagcgattct cattccaatg tactttttga tcggcatctg gggcgggccg
      361 cgccggattt acgccaccat caagttcgtg ctcttcacta tgaccgggtc gcgtttgatg
      421 ttcgtggcca tcatcgtcct gttcttcctc taccacagcc acaccggcca gtattcgttc
      481 aacctgcttg actactacaa attgccgatt cctctgggcg cgcagacctg gctttttctg
      541 gcgtttgcgc tggcctttgc tatcaaagtg ccgatgttcc ccttccacac ctggctgccg
      601 aatgcgcacg tggaggcgcc cacggcgggg agcgtcatct tagcgggcat tctgctcaag
      661 atggggacgt acggattctt gcgcttctgc tggccccttt tcccgaatgc gttcgagcag
      721 ttcttgccga tgatgatcgt cctttctctc atcgggatca tctatggtgc gtggatgtca
      781 atggtgcaga aagacatcaa gagtctgatt gcctactcgt cggtgagtca tctggggttc
      841 gtgatgctgg gtttggccgt gctcaatctt caaggtctcc aggggagtct cttgcagatg
      901 ctcaaccacg gcatttccac cggggcgttg ttcttaatcg tcgggatgat ttacgagcgc
      961 agacataccc ggctgatcgc cgactacggc ggcattacca aggttgttcc ggtttgcgct
     1021 acctttttcc tgatcgttgc tctgtcatcc atcggcctcc ccggcaccaa cggctttgtg
     1081 ggtgagtttc tcatccttct gggcgatttt aaattcaact ggatttacgc cgctctggga
     1141 gcgaccggcg tcatcttcgc ggccgtctac atgctctgga tgtaccagcg ggtgatgttc
     1201 ggcccggtga ccaatccggc caactcggca ttaacggacc tcaaccggcg tgagcggtgg
     1261 acgttaatcc ctttggtcgt tctcattttc tggatcggcc tctaccccaa gccgtttctc
     1321 tcccggatgg agggttcgct tgccaacctc gtcacgcagg tggaggagag gcgggggtat
     1381 ctcagtcaat accgtaatgg tgaaatcgta tcaccagtgc cccaaggtct tttaacttcg
     1441 acacctcaac accccgacac ctcgacactt aatcaagcga gccggccatg actattactc
     1501 ctcccgatct cgttttcagc ggcttggtgc cggaaatcgt cgtcgtgggt ttcgcaaccc
     1561 tcttgatggt cgtggggccg tttttcaagc agcgcggcca gggcgtagtc ttctggctta
     1621 cgcttatcgg tctggctttg gccgcgttca ccagcatttt gctatggaat caaaccattt
     1681 ccgccatggg tgggatggcc tactccgaca acttcgccac cttcttccgg ctggtgtttc
     1741 ttctggcggc gttcgtcgtc gttcttttct caaaagctta cctcaaatgg gagaatctgc
     1801 cggcggcgga atactattcg cttttgctct tttctacctt cggcatgatg cttatggccg
     1861 gcagtctcaa cctgatcgtg attttcctgg ggttagaagt gatgtcgatc ccgctttaca
     1921 ttttggccgg ctaccggcgc agcgattttc gttcgattga atcgtcgatg aagtactttc
     1981 tgatcggcgc cttcgccagc gggtttttgc tctacggcat tgcgcttatc tacggtgcgg
     2041 tcggctccac cgatttggag aaaattgcgc aggcgatttc ttccggtcag gtggcgaccg
     2101 actttctctt tctctccggc gtcgggttgc ttctcgtcgg cttcggcttc aaagtggcct
     2161 cggtgccctt ccatatgtgg acgccggatg tctatgaagg cgcgccgacc gcggtcaccg
     2221 gctttatggc cgccgggccc aaggcagccg gctttgccgc tctgctgcgg gttttttcga
     2281 tttttttggc cgggctcaat cataacctca tgccggcgtt ctggattctt tcggtcttga
     2341 ccatgaccac cggcaacgtc atcgccctgt cccagcccaa cctcaagcgg atgctggcct
     2401 actccagcat agcccatgcg gggtacattt tagtggccct ggtggccggg ggagaagcgg
     2461 gcattgccag cgcgctcttc taccttctgg cctacagcct catgaacctc ggcgctttcg
     2521 cctgcgtctg tgcggtggaa aaagtcaccg gcagggggaa tgaaattgcc tcctacgccg
     2581 ggctggcgtc ccggcacccg ctcgttgcga tagcgctgac cattttcctg gtctccttag
     2641 gcggactgcc gcccaccgcc ggttttgtgg ggaaatttta cgtcttctcg gcggcggtga
     2701 atgcgggcca gattccctta gtcatcatcg ccgtgctcaa cagtttgatt tcggtctact
     2761 actacctgcg gccggtggtg gtgatgttca tgcaggcgcc ggaggcagtt cctgcttccg
     2821 cgccagaggg ttccgcgaca gctttcgcct ggccggtatc tttagcccta tcgattttca
     2881 tcactgcctg ggggacgctg cagctgggga ttttcccgca ggcgctttta gagttggcgc
     2941 gccagacggt gtcggcattc ctttaatccg cctttgcaca agggctgtta acgacatatc
     3001 ttctcattag actttgatgc gcgatctggt aaaaaatcgc ttgcagttgc tctcccagcg
     3061 agtcaaaaag cgcagactgc gcttgacccg tcagcggacg atcgtgctcg aagaattggc
     3121 gaaagtcgac acccacccgc gcgccgacca actgtaccga atggtcaggc agcggctgcc
     3181 gacgatttcg ttcgggaccg tctaccgcaa cctgaaaaca ctctgccaac tggagctggt
     3241 gaccgagttg aaattgggcc aggattcttc ccggttcgac gcccgcaccg atccccacca
     3301 ccacgtcgcc tgcacgcatt gtggttgcgt ggtggatgtc gaactgccgg tggagtccgg
     3361 cttgcagaaa aaggcggccc ggcagaccgg ctttgcggtt tcgcactacc agaccgcctt
     3421 cttcggcacc tgcccccgct gcaggggcaa gtcctgattg tgtgattttt tctcacttta
     3481 tcccgtattt ctggaagagt gctgtctaaa gttttcttgg ctattggggt atggggcgtc
     3541 gccgcaatgg tgcaggcggg tgacaagccc gcttacactt tacccggcgc catcgacctg
     3601 gcccaaaggc aaaatcccga agtgctgcaa gctcggcagc aaatcgaact ggcgcggggc
     3661 cgaaagcttc aggcctatgc gctcaccccc tggcgattct tttggccagg ccggtgaccg
     3721 ggttgttgaa gtcgggca
//
LOCUS       DBNGZD010000384         5500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1657613, whole genome shotgun sequence.
ACCESSION   DBNGZD010000384 DBNGZD010000000
VERSION     DBNGZD010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1657613"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC16GM_10070"
     CDS             complement(<1..268)
                     /locus_tag="AC16GM_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924052.1"
                     /translation="MTRLRYTLAALGLFLLAGVVGPRVLAQEENDTARVSGEAERAEL
                     QNIRNQIASQKTTLTTLEKKEGTLSAQLAHLEEKLRLSDQLIQRL"
     gene            complement(265..1128)
                     /locus_tag="AC16GM_10075"
     CDS             complement(265..1128)
                     /locus_tag="AC16GM_10075"
                     /inference="COORDINATES: protein motif:HMM:NF037183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR2924053.1"
                     /translation="MTPFYHTKESFRWLSHSLGATLLTIVAFAVVVVLFFLGWQANGK
                     VEAKLLQLEERFVLSVVLADDLPPGTEEELTRQLERLPAVAAVAFQSKAEALSALEKE
                     LETEMGTELAANPLPASFRLQLKREAFTTDRLAQLVTTVRSFSGVREVIYPETWAEQY
                     GRLAAVLGSGRLAFLGIFVALAGLLVAAAVRQLFCDRKDDVFTLRLLGAPWSFALAPY
                     LVAGGVIGLGGGLFGLLAAWGLTTGFSRMAGIRFGPLLPGGYFFAGAAVLLSMLLIGT
                     APLMFRRHSPP"
     gene            complement(1125..1880)
                     /locus_tag="AC16GM_10080"
     CDS             complement(1125..1880)
                     /locus_tag="AC16GM_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2924054.1"
                     /translation="MHENLLLEVTGLACSVRVDADRHQKPVFTDISFSVGSGEIVGIV
                     GPTGAGKTSLFNCLVGLAQPAAGLITYAGTVLPASPKPGPRLRRHLGLVFQDDHLFFD
                     RTVFDNIALPLLLLKKSSPEIRRRTTAVATRIGLSAVLLSRCGSLSAGERRLVALARA
                     VVHEPPLVLADEPLGPISPPAGEVLGLFRELGDKGTGFLLFSSVVDLIEPYCTRLHVL
                     KEGSLVPVAAATPPESPRPISIPLEPSSPAVSA"
     gene            complement(1892..2944)
                     /gene="pdxA"
                     /locus_tag="AC16GM_10085"
     CDS             complement(1892..2944)
                     /gene="pdxA"
                     /locus_tag="AC16GM_10085"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR2924055.1"
                     /translation="MREKIITLTMGDPAGIGPEVTLMALAQRGMYRGWRPVVFGSLPI
                     LTKLAKRFAPKVTLVPVEGESYPHLKANTVGVQDVSTKENVAIEPGKLSAEAGRLAGR
                     AIEEAVRFVLRHRLPALVTAPVNKQNLSLAGYRFTGHTEYLAQATGVADSAMFFVTGD
                     VKVVCVTTHIPLAEVTRVLTPELIERKILLAADALRNYFGLKRPRVALCGINPHAGDG
                     GLLGSDEAGLIEPVIRRLSQRGMAISGPYSADSVFLPQVRRKFDLIAAMYHDQGMIPV
                     KLLGRGRAVNITLGLPFVRTSPAFGSAFDIAGKGQADAAGMVAAIRWAVRLGEIELRR
                     WRTKSQVRRVQPITDR"
     gene            3318..>5500
                     /locus_tag="AC16GM_10090"
     CDS             3318..>5500
                     /locus_tag="AC16GM_10090"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR2924056.1"
                     /translation="MRVRTLLLTLALCSALGGLAATALAAVPSLINYQGKLTDNSGAP
                     ITSAVTLKFRIYDAPTGGTLLWGPESHPTTPDANGIYNVILGAGTPPVPFPANLFADA
                     SRYLGVEVNADGEMTPRQQITAVAYSLRVATVDGATGGTISGDVSIASDLSVSGKATI
                     GPGHTNTGPFAFVAGANNRARGAYSVVSGGGGTAFGLVDSNSAIGDYSTIGGGLRNTA
                     IGTVATVGGGQNNTASGEVATVGGGLADTASGHYSIVSGGFHNVGSGDSSVIGGGSSN
                     RASALVATVGGGQINTASATGATVGGGGNNRARGQFSTVAGGGGAADSDSNSASGNFS
                     AIGGGRRNTASASSTVGGGESNTASNSFATVGGGGFNKARGAFSVVGGGGGGAAVDSN
                     SASGSFSAIGGGRRNIASGSFSTTVGGGESNTASASGATVGGGGNNKALGSFSVISGG
                     GGSTTGDSNVASGGTSAIGGGFRNTASGSGATVSGGSLNTASNSGSMVGGGSNNTASG
                     INATVGGGNNDTASGGVSTVPGGSGNTARGDFSLAAGRRAKANHAGAFVWADDNSFDF
                     ASTAAKEFSARTTGGARFVSAIDGLGNPTAGVTLAAGGNAWAGISDSTKKRNIRLVDT
                     KEILAKVVSLPVKRWSFKSQDPKIEHIGPMAQDFYALFGVGEDDKTISTIDPPGIALA
                     AIQELHKVTQEQRAEINGLKAQVSELQELVQTLLAERQEKQASDY"
BASE COUNT         1169 a         1687 c         1635 g         1009 t
ORIGIN      
        1 tgaggcgctg gatcaattga tccgacaggc gcagtttctc ctccaggtgc gccaactgtg
       61 ccgaaagcgt cccctctttt ttctccagag tggtcagggt ggtcttctgg gaggcgattt
      121 gattgcggat gttctgcagt tcggcccgtt ccgcctcgcc ggaaacgcgt gccgtgtcat
      181 tttcttcctg agccaatacc ctcggcccga ctaccccggc caggagaaaa agtcccagtg
      241 ctgcgagggt gtagcggagc cgcgtcatgg aggagaatgc cggcggaaca tgagtggagc
      301 ggtgccgatc aagagcatac tcaaaagcac cgccgcaccg gcgaagaagt agcccccggg
      361 caaaagcgga ccgaaccgaa ttcccgccat ccgggaaaat ccggtggtca ggccccatgc
      421 ggccagaaga ccaaaaaggc cgccgcccag gccgatcacc ccgccggcca cgagatatgg
      481 ggccagagca aacgaccacg gcgcgcccag aagtctcagg gtgaagacgt cgtccttgcg
      541 gtcacagaag agctggcgca cggcggccgc gaccaataat cctgccagag caacgaaaat
      601 acccaaaaac gccaaccggc ccgagcccag caccgctgct aaccggccgt actgctcggc
      661 ccaggtttcc gggtagatga cttcccgcac accggaaaag gagcgcaccg tcgttaccag
      721 ttgggccaat ctgtccgtcg taaaagcctc tcgcttcaac tgcagccgga atgacgccgg
      781 caacggattg gccgccaatt ccgtccccat ctcggtttcc agttcttttt ccaacgccga
      841 cagcgcctcc gctttggatt gaaaggcaac cgcagcgacc gctggcagac gctctaactg
      901 acgggtcaac tcctcctcgg tgcccggcgg caggtcgtcg gccaagacga cggagaggac
      961 gaagcgctct tccagttgca aaagcttcgc ctccactttt ccattggcct gccagccgag
     1021 aaaaaagagc actaccacaa ccgcaaaggc aactatggtc aatagggtgg ccccgagact
     1081 gtgagacagc cagcggaagc tttctttcgt atggtaaaag ggcgtcatgc cgagaccgca
     1141 ggcgaggatg gttccagggg aatcgatatc ggccggggcg attccggggg ggtggcggct
     1201 gcaaccggaa cgaggcttcc ctctttgagc acgtggagcc gggtgcagta cggctcaatc
     1261 aaatcaacca ccgacgaaaa cagaagaaac ccggtgcctt tgtccccaag ttcccggaac
     1321 aaccctaaaa cttctcccgc cggcggcgag atcggcccca gcggttcatc ggccaaaacc
     1381 aaaggcggct cgtggaccac cgcccgggcc agagccacca ggcgcctttc cccggcggaa
     1441 agcgaaccgc accgggagag caaaaccgcg gaaagcccga tccgcgtggc aacggccgtg
     1501 gtgcggcggc ggatttctgg cgaacttttt ttcaaaagca gaaggggaag cgcaatgttg
     1561 tcaaaaacgg tccggtcgaa aaagaggtgg tcgtcctgaa aaactaagcc caggtgccgc
     1621 cgcagccggg gacccggttt cggcgatgcc gggagaaccg tcccggcata ggtgatcaac
     1681 ccggcggcag gttgcgcaag accgaccaga cagttaaaaa gcgaggtctt cccggccccg
     1741 gtcggaccca caatccccac gatctcacct gacccgaccg aaaaagaaat gtcggtgaat
     1801 accggtttct gatgtcggtc agcatcgact cgtaccgagc aggccagtcc ggtgacttcc
     1861 aataaaaggt tctcgtgcat cgtggctata actacctatc ggttatcggc tgaactcttc
     1921 tcacctgtga tttcgtcctc caacgccgca gttcgatttc tcctagtcgg actgcccagc
     1981 ggatggccgc caccattcct gccgcgtcgg cttggccttt acccgctata tcaaacgcgc
     2041 tgccgaaagc gggggaggtg cggacaaacg gcaatcccag cgtgatgttg accgcccggc
     2101 cccggcccaa aagtttcacc ggaatcatcc cctggtcgtg atacatcgcc gcaatgagat
     2161 cgaatttgcg ccgcacctgc ggaaggaaaa cgctgtcagc cgagtagggg ccggatatgg
     2221 ccatcccccg ctgtgacagt cgtcggataa ccggctcaat gagtcctgct tcatcacttc
     2281 ctaatagacc gccgtcgcct gcgtgcgggt tgattccgca gagcgccacg cggggccgtt
     2341 ttagcccaaa atagtttctc aaagcatcgg ccgccagaag aattttccgc tcgatcaact
     2401 ccggcgtgag aacgcgagtg acttcggcca ggggaatgtg ggtggtgacg caaaccactt
     2461 tcacgtcgcc ggtcacaaag aacatcgcgg aatcggcaac gccggtggcc tgcgccagat
     2521 attcggtatg gccggtgaag cggtacccgg ccagggataa gttctgcttg ttcaccggcg
     2581 ccgttaccag cgctggcaac cggtgccgca aaacgaaccg caccgcttct tcaattgccc
     2641 gtccggccag ccgccccgct tcggcgctca gttttcccgg ctcgattgcc acgttctctt
     2701 tcgttgatac atcctgcacg ccgacggtat tggctttgag atgcggatat gactcgccct
     2761 ccaccggcac gagtgtgact ttcggcgcaa accgtttcgc caatttcgtg aggataggta
     2821 acgacccgaa caccactggc cgccagccgc ggtacatccc gcgctgcgcc agtgccataa
     2881 gcgtcacctc cggcccgatc cccgccgggt cgcccatggt gagggttatt atcttctctc
     2941 gcacagggtt aagaaagcga gtggatgaac ttgaagcaac cgagaagacc tgcgcctctc
     3001 ggtacaccag tccataaata ggacaaaact aatccaaaac gcgcttgaca agttccagcc
     3061 agcacttttt ctttcccacc ggagggaggg gcgacagctg gtaattgata acccgtggcg
     3121 ggctttgaga ttcagccagg gttgagtgtg catcggcaac cgggaggtgc gatggcttat
     3181 tctttcttgg gttagactcc aaacgccggt caccaaaggt gatttctccg ttcacagatt
     3241 caccgggtct gcaaacgact caacacaggg tcgaagaccc agtcttaacg taagactgaa
     3301 ctttggggag gcttaccatg cgcgtacgca ctttgctttt gacgctggca ctttgctccg
     3361 ccttaggcgg attggcagca acggctttag ccgcagtgcc aagtctgatc aactaccagg
     3421 ggaagttgac tgataactct ggcgcgccga ttacctcggc cgtgaccttg aagttccgca
     3481 tctatgacgc tccaacaggc ggaacgcttc tatggggccc cgaatcacac cccacaacgc
     3541 ccgatgccaa cggcatctac aatgtcatac tcggtgcggg gacaccaccg gtgccctttc
     3601 ccgccaacct gttcgcggac gcctcgcgct acttaggtgt ggaggtgaat gctgacgggg
     3661 aaatgacccc ccgccagcag atcactgcgg tagcgtattc tctgcgcgtt gccaccgtcg
     3721 atggcgccac cggcgggacg atttccggtg acgtgagcat tgccagtgac ctctccgttt
     3781 cgggcaaggc taccattggc ccggggcaca ccaacaccgg cccattcgcc tttgtagccg
     3841 gggccaacaa ccgggcgcgg ggcgcctact ccgttgtgag tgggggcggg ggaactgctt
     3901 tcggcctggt tgactccaac tcagccatcg gcgactattc gaccattgga ggcggcctgc
     3961 gcaacaccgc catcggcact gtggccactg tcggcggcgg ccagaacaac accgccagcg
     4021 gggaggttgc cacggttggc ggcggcctgg ccgatacggc cagcggccat tattccatcg
     4081 tcagcggcgg ttttcacaac gttggcagcg gggacagttc ggtaattgga ggcggttcca
     4141 gcaacagggc cagtgctctt gttgccacgg tcggcggcgg ccaaatcaac acggctagcg
     4201 ctacaggagc cacggtcggt gggggcggta ataaccgggc gcgtggccag ttttccactg
     4261 tagccggcgg cggcggagcc gcagattccg actccaactc ggcttctggc aatttttcgg
     4321 ccatcggcgg cgggcgaagg aataccgcca gcgcttcgtc aacggtcggc ggcggcgaga
     4381 gcaacaccgc gagcaactct ttcgccacgg tcggcggcgg cggatttaac aaagctcgag
     4441 gggccttctc cgtcgtgggc ggcggtggtg ggggcgcagc tgtcgactct aactcggcct
     4501 cgggcagctt ttcggccatc ggcggcgggc gaaggaacat cgccagcggc tctttctcaa
     4561 caacagtcgg cggcggcgaa agcaacaccg cgagcgcctc tggtgccacg gtcggcggcg
     4621 gcgggaacaa caaagctcta ggatcatttt cagttattag cggcggtggc ggatccacta
     4681 ctggagattc aaacgtggct agtggtggaa cttcggccat cggcggcggc tttcgcaaca
     4741 ctgcaagcgg ttctggtgcc acggtcagcg gcggcagcct aaacaccgcg agcaacagcg
     4801 gatcaatggt cggcggtggc agcaacaaca ccgcgagcgg gattaatgcc acggtcggtg
     4861 gcggcaacaa cgacaccgcg agcgggggtg tatctactgt gcccggtggt tctggtaaca
     4921 cagcacgcgg cgatttttcc cttgccgccg ggcgtcgggc aaaggcgaac cacgctggcg
     4981 cttttgtctg ggccgatgac aacagctttg attttgcttc aaccgcagca aaggagttca
     5041 gcgctcgcac tactggcggc gcgcggttcg tctccgccat tgatggcttg ggtaatccca
     5101 cagccggtgt gaccctggct gccgggggca acgcctgggc gggcatttcc gatagcacca
     5161 agaaacgcaa catccgcctg gtggatacaa aagaaatcct ggccaaagtc gtctcgctcc
     5221 ccgtcaaacg ctggagcttc aagtcacaag acccaaaaat cgagcacatc ggcccgatgg
     5281 cgcaggattt ctacgcgctt tttggtgttg gggaggacga taagaccatt tccaccatcg
     5341 acccgcccgg catcgcctta gctgccattc aggaattgca caaggttacg caggagcagc
     5401 gagccgagat caatggactc aaagcacaag tgagcgaatt gcaggaactc gtccagacgc
     5461 ttctggctga gcggcaagaa aaacaagcga gtgattacat
//
LOCUS       DBNGZD010000385         4669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_269623, whole genome shotgun sequence.
ACCESSION   DBNGZD010000385 DBNGZD010000000
VERSION     DBNGZD010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_269623"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="AC16GM_10095"
     CDS             complement(<1..939)
                     /locus_tag="AC16GM_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR2924057.1"
                     /translation="MQKKILLVTASLLLILAAGIEPQPALPGEKGDPTSQGQLQDPRE
                     KHLKNIRQLTFGGENAEAYFSADGRILSFQRTSETWPCDQIYTMTTTGDSLRLVSTGK
                     GRCTCAYISPDGERIIFASTHLFGDSCPPKPDYSQGYVWSLYKSYEIFSAKPDGSDLV
                     RLTNDNSYDAECVFSPDGKKILFTSDRDGDLELYAMDAGGKNPQRLTHSPGYDGGAFY
                     SADGKMICFRAQVIKDSTELRDYQTLLAKGLIRPHTLELFVMNADGTGRKQITQNGSA
                     NFCPFFSPDGKKIIFASNMNDPKGRNFDLYLINIDGT"
     gene            complement(977..1114)
                     /locus_tag="AC16GM_10100"
     CDS             complement(977..1114)
                     /locus_tag="AC16GM_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924058.1"
                     /translation="MFRARWQRWRQKGWQFALLLVLAYLVRDLFLYVVLPVFALQTVC
                     R"
     gene            1306..2703
                     /gene="hslU"
                     /locus_tag="AC16GM_10105"
     CDS             1306..2703
                     /gene="hslU"
                     /locus_tag="AC16GM_10105"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR2924059.1"
                     /translation="MKELTPKEIVKELDKYIIGQDKAKRSVAIAMRNRWRRQQLGEEL
                     RQEVMPNNILMIGPTGVGKTEIARRLAALANAPFIKVEASKFTEVGYVGRDVESMVRD
                     LVDLSVNTVKREQQAKVQQKAQQLAEDRVLDLLLPGDHKRKPVSTPVAVGTGETLVEM
                     TPEPDLASDNDTWEQTREKLRGQLRDGKLEKRLVEIEVPNHAFPVIEIFSPMGMEELG
                     INFQEMFSGMMPKKTKRRRMSVGEAQRFLVQEEAQKLIDMDEVIGLALDRVENSGIIF
                     VDEIDKIAGEKSKEGPDLSREGVQRDILPIVEGSNVMTKYGMVKTDHVLFIAAGAFHV
                     SKPSDLIPELQGRFPIRVELDSLTTKDFVRILTEPKNALIKQYTALLATEGVEVSFSK
                     DAIQEIAAIANQVNERTENIGARRLHTVMTTLLDNLLFEASELKGKKVKLTRESVRKA
                     LKEIVEDEDLSRYIL"
     gene            3199..>4669
                     /locus_tag="AC16GM_10110"
     CDS             3199..>4669
                     /locus_tag="AC16GM_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014544.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HIR2924060.1"
                     /translation="MSNSTLAATVVTDRPDYAPGETVVITGSGFAASESVTVRVTHVD
                     TTIIGGAGHDPWTVKASPAGGFTTYWVVPFDDNVGQTLKVTATGKTSGLVATTTFNDV
                     NSKITITSIPSTVVAGDTFMVTARLVQNCGGAVDSPLVNRTVLFFLTEGNCGNDVGQV
                     APDSDQTDANGFATGKLVIPFAGSWGVRVKYRGEDKPDPCPTPGNSACDPFDPAANKR
                     CVDISSSNDCKGIQAIACTPPVITCPNDTTVCNGTGQCSRIVNYPSPTTTAGCGPVTV
                     ACTPASGSAFPVGMTTVTCIASDTVGKKDTCTFKVTVNDCQKPTITCPANIVKPNDPG
                     QCCAVTTFAPTVSDNCPGVTFVCTPASGFCFPVGVTTVKCIVTDAASNKDSCTFTVTV
                     NDTQKPTITCPAPIVKPNDPGQCCAVATFAPTFSDNCPGATVVCTPASGFCFPVGVTT
                     VKCIVTDASSNKDSCTFTVTVNDTQKPTITCPAPIVKPND"
BASE COUNT         1067 a         1192 c         1371 g         1039 t
ORIGIN      
        1 cgtgccgtcg atgtttatca agtataagtc aaagttgcgg cctttgggat cgttcatatt
       61 tgacgcgaaa atgattttct tcccatctgg cgaaaagaag gggcagaagt tcgccgagcc
      121 gttctgggtg atttgtttcc ggcccgtgcc gtcggcgttc attacgaaca gttccagtgt
      181 gtgcgggcgg atcaaacctt tagccaaaag cgtctggtag tcgcgcagtt cagttgagtc
      241 tttgatcacc tgcgcccgga agcagatcat tttgccgtcg gctgagtaaa aagcgccgcc
      301 gtcgtagccg ggagaatgag tcagacgttg gggatttttg ccgccggcat ccattgcgta
      361 cagttccagg tcaccgtccc ggtcggaagt gaaaagaatt ttcttcccgt ctggggagaa
      421 gacgcactcg gcgtcgtaac tattgtcatt cgttaaacgc accagatcag agccatcggg
      481 tttggcagag aagatttcat aacttttgta aagcgaccag acgtacccct gcgagtagtc
      541 gggcttgggc gggcaggagt cgccgaagag gtgggtcgag gcgaagatga ttcgttcgcc
      601 atccggcgaa atgtaggcgc aggtgcagcg ccccttgccg gtggagacca gccggagcga
      661 gtcgcctgtg gtggtcatgg tgtaaatctg atcgcagggc caggtctcgc tggtccgctg
      721 gaaactcagg atgcgtccgt cggcggagaa gtaggcctcg gcgttttcac cgccgaaggt
      781 gagctgccgg atgttcttca ggtgcttctc gcgggggtct tgcagttgac cctgtgacgt
      841 cgggtcgcct ttttctccgg gcaaggcggg ttgtggttca ataccggctg ccaagatgag
      901 cagaagggac gcagttacca gcagaatttt cttttgcatt gggtttgtcc ttttcgttag
      961 ggtcattgcg tagtcgctac cgacagacgg tttgcaaagc aaagaccggg agcaccacgt
     1021 acaggaaaag gtcgcggacg aggtaagcca gcacgagcag caaggcgaac tgccagccct
     1081 tctggcgcca gcgctgccag cgggcgcgga acatttttgg cgcaggttcc gtgggcgcag
     1141 gaatttcccg gggagcggat ttcctccaga ttgcgaactt cactcaaagc caaatacgag
     1201 aaccaggtca agaaaagata tgacgcagat catctccgcg gttcgccatt ggactggttg
     1261 cggttacgta ttattatggt tacgctaatg agaggagcag aaaaagtgaa agaattaacc
     1321 cccaaagaga tcgtcaaaga attagataaa tacatcatcg ggcaggataa agccaagcgc
     1381 tcggtggcga ttgccatgcg caaccgctgg cgcaggcagc agttgggcga agagctgcgc
     1441 caggaggtga tgcccaataa tattctcatg atcgggccca ccggcgtggg caagaccgaa
     1501 atcgcccgcc ggctggctgc gctggccaac gcgcccttta taaaggtgga ggcctccaag
     1561 ttcaccgaag tgggctacgt gggccgggac gtggagtcga tggtccggga cctggtggat
     1621 ctatccgtca atacggtcaa gcgggaacag caggccaaag ttcagcagaa agcccagcag
     1681 ttagccgaag accgggtttt ggacctcctt ttgccgggcg atcataagcg taagccggtg
     1741 agcacaccgg tcgccgtcgg gacgggtgaa actttggttg agatgacccc cgagccggat
     1801 ctggcgtccg acaacgatac ctgggagcag accagggaaa aattgagggg gcagctgcgg
     1861 gacgggaaac tcgaaaagcg gctggttgaa attgaggtgc ccaaccacgc ttttccggtg
     1921 attgagattt tctccccgat gggaatggaa gaattgggga tcaacttcca ggagatgttc
     1981 tccggcatga tgcccaaaaa gaccaagagg cggcggatgt cggtgggcga agcacagcgt
     2041 tttctggttc aggaagaggc ccagaaattg atcgatatgg acgaggtgat cggcttagca
     2101 ctcgaccggg tggagaactc aggtattatc tttgtagacg aaattgacaa gattgccggg
     2161 gagaagagta aggagggccc tgacctctcc cgggaggggg tccagcggga catcctcccc
     2221 attgtcgaag gctcgaacgt gatgaccaaa tacgggatgg tcaaaaccga ccatgtcctc
     2281 ttcatcgcgg ccggggcgtt ccacgtctcc aagccctccg acctgatccc cgaactccag
     2341 ggccgcttcc ccatccgggt ggagttagac agtctcacga ccaaagattt tgtccggatt
     2401 ctgaccgagc cgaagaatgc cctgataaaa caatacactg cccttttggc aaccgagggg
     2461 gtggaggtgt cgttctccaa agacgccatt caggagattg ccgcaattgc caatcaggtc
     2521 aatgagcgga ccgaaaatat cggcgcccgg cggctgcaca cggtgatgac caccttgttg
     2581 gataacctgc tttttgaagc ctcggagctg aaaggcaaaa aggtgaaatt gacccgcgaa
     2641 tcggtgcgca aggccttaaa agagatagtg gaagacgaag acttgagccg gtatatcctt
     2701 taaaaatgac cgcaacttta ataggtttaa tgggttagcg ggagggaaca tttttttctt
     2761 tattgtaaaa aagtacttga caagttttag ccaggcgtta ttttatagtt gcgtttaggt
     2821 caaagcacaa agtaaccaaa tcccactgaa aaagccccac acggggcaac ttaatatttt
     2881 cgccagcatt ttttagttta cctgcgcacc gcaaagttcc ttggttttgc gggcagatga
     2941 gttgcatcag tgaattttgc agtgcgcagg cgaggcgaaa agggagttaa ccaagcaagc
     3001 aggagaacaa tgaaggtatt ccgagatggg ttgtaggaga tttcggcaga gaagttggat
     3061 tacgcaaaac cagactgata cgaaggtaag aacggtttcc ttgcttctcc tgcggggggt
     3121 gctatgcggc aactgagatg gggtggtttg tcttgttctc ttttctttct gatcctgctg
     3181 gggggctatc taactggctt gtccaattca accttggccg ccactgtggt gaccgacagg
     3241 cctgattatg ctcctggcga aacagtcgtc attaccggca gtggttttgc cgccagtgaa
     3301 tcagtcacgg tccgggtcac ccatgtcgac acgaccatta tcggtggcgc cgggcacgat
     3361 ccctggacgg ttaaggcctc gccagcgggt ggtttcacca cttattgggt tgttcctttt
     3421 gacgacaatg tcggccaaac cctgaaggtt accgcaaccg gcaaaacatc gggtttggtg
     3481 gcaacgacga cattcaatga cgttaatagt aaaattacta taacttcgat tccatccacg
     3541 gttgtggcgg gtgacacctt tatggtcacc gcccggctgg tgcaaaactg tggcggcgca
     3601 gttgactcgc ccttggttaa ccgtaccgtc cttttctttt tgaccgaggg caactgcgga
     3661 aacgatgtcg gacaggttgc tccggacagc gatcagaccg acgccaatgg atttgccacc
     3721 ggcaaactgg tgattccctt tgctggttcc tggggcgtgc gggtcaagta ccgcggagag
     3781 gacaaacctg atccctgtcc cacgcccggc aacagtgcct gcgatccttt tgatcccgct
     3841 gccaataagc ggtgtgttga tatttcctct tcgaacgatt gcaaaggcat ccaggcaatc
     3901 gcatgtaccc cccctgtgat tacctgtccg aatgacacta cagtttgtaa tggcactggt
     3961 cagtgttcca gaatcgttaa ttatccttct ccaaccacca cggctgggtg cggcccggtt
     4021 accgtagcct gcactcccgc atctggtagt gcctttccgg tgggaatgac cactgttacc
     4081 tgcattgcca gcgatacggt gggcaaaaaa gatacttgca catttaaggt taccgtcaac
     4141 gactgccaga agccgaccat tacctgtccg gccaacatcg tgaagccgaa cgatccaggt
     4201 cagtgctgtg cagtaacgac ctttgctccc accgtcagcg acaactgccc cggggttacc
     4261 tttgtctgca cgccggcgtc ggggttctgc ttcccggtgg gggtgacgac ggtgaagtgt
     4321 atcgtcaccg acgctgccag caataaagat tcctgcacct tcacggtgac ggtgaatgat
     4381 acgcagaagc cgacgatcac ctgtccggcg cctattgtga agccgaacga tccgggtcag
     4441 tgctgcgcgg tggcgacctt tgctccgacc ttctcagaca actgtccggg cgcgacggta
     4501 gtctgcacac cggcgtcggg attctgcttc ccggtggggg tgacgacggt gaagtgcatc
     4561 gtcaccgacg cttccagcaa taaagattcc tgtaccttca cggtgacggt gaacgacacg
     4621 cagaagccga cgatcacctg tccggcgcct attgtgaagc cgaacgatc
//
LOCUS       DBNGZD010000386         9909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_616915, whole genome shotgun sequence.
ACCESSION   DBNGZD010000386 DBNGZD010000000
VERSION     DBNGZD010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_616915"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2502)
                     /locus_tag="AC16GM_10115"
     CDS             complement(<1..2502)
                     /locus_tag="AC16GM_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2924061.1"
                     /translation="MGLPSALVESWNRHQGQTLLPLQVSAVDSGILTGQSLLIAAPTS
                     SGKTFCGELALAAAVARRKKAVFLVPLKAIAEEKFLDFSQKYAPLGLKVVVSTKDRRA
                     FDGPIERGQFDLAILIYEKFNQLLIRNLDLLSLIDLLVIDEIQMLDDPTRGPVLELAL
                     LKVLSFAKRPQIVALSAVLEDAAPLADWLGCRLLVERKRPVELFTGVLLESRFAFRAF
                     NTKEEGEEQWEVESAAGEDKIDADQRARLVTQIQKCVAGGDPVLVFLKGKRDCQGLAQ
                     LLAEAAPWPPAAGALEEIEAEPEVVLSESLAYCLRRGVAFHHADLPFSLRRIVEHSFL
                     AGEIKIVCATTTLALGVNLPAQTVFVEPWKYRNGPQTNRPVLVPLSVAEFENMAGRAG
                     RLNLAPSGRAVLLAKTPLEQEVLWSNYVTGRREKIASRLSHLPLGDIVLDLVAAESVK
                     TVEELEATLLKSFHWVQKTPDLSPTLSSKEREGFCSSLPLRGGQGGVSPKDIKRAIED
                     LAGRGLLTQHDNGSIAATSFGKKVAQTGIRVATGKAIEEACSSKTRFDALDWFYFLCG
                     TEEAANLPVTLNFHERTSDYYHGLWLKEKTNFPPDSGPLVSGQMVVRLKLALLLSDWI
                     DGVVTFDLEKKFFSPAGTIQQVAETLEWLLDAAAQMALVLCPGQKLSPELKSLSRRVK
                     WGAREELLPFFDALGPAAGRDHLLKLVASGWKTIEGLKQATVEELRGVVPQKIAERIV
                     AHLERPQAGAPAKEKSHPARAVPEQGGNQNPRKAGLWLVGVPKKQRWTVEIDGQPVLL
                     SNKSFRFLALLAFARVERPDGWLSKEELEPGDYQAR"
     gene            complement(2721..2969)
                     /locus_tag="AC16GM_10120"
     CDS             complement(2721..2969)
                     /locus_tag="AC16GM_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924062.1"
                     /translation="MPGSCCVKLSASESRIDPRPVEIEEDEKDEIDDSLLLRHLHPEV
                     ANRPETLPTCPECNEELVAAPGLFFCLACGKNYCVENP"
     gene            complement(3175..3450)
                     /locus_tag="AC16GM_10125"
     CDS             complement(3175..3450)
                     /locus_tag="AC16GM_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924063.1"
                     /translation="MSNRDTPTLSPILHRTVQVKAIVVGSRVTPLWMRYNGRVFPFQQ
                     VLRSWREENGLTFPRWMFHLTDGLNFFELSWDTTDFCWRLWRMESQE"
     gene            3885..4481
                     /locus_tag="AC16GM_10130"
     CDS             3885..4481
                     /locus_tag="AC16GM_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2924064.1"
                     /translation="MPQEVIMRKGKLIISICLLAVFCAGAVQAGDLGIGVKVVTGSHS
                     IQVTNLTLTIPFVGTVAMSPLPPAKGPNDSSTVTYSLAQYGYNPTGASVSFLMDGVPH
                     SGSYSPLRYDTLYTLPFHGLPDSKISFHLCSLRGDVNNDFYTDISDIVASIQYVVFGT
                     ALPDLTAGDVNGDGVVDITDIVGLVQYVVFGGPVPICL"
     gene            complement(4686..6263)
                     /locus_tag="AC16GM_10135"
     CDS             complement(4686..6263)
                     /locus_tag="AC16GM_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HIR2924065.1"
                     /translation="MPTRGERVHLKTTCLFATVLTFLFALPARADQLRITNYESRALT
                     PPCSLQQVYRSQADTADQHWGHAVSGLGDVNNDGVPDFIIGVHAADPNGIAEAGEVIV
                     FSGATNLPLYQVAGADTGNEFGSTLAAVGDLNGDGKPDFVAAAPSAERFGALSAGTAG
                     LYSGVNGALIRSFVGDSADNQSGLPTQFGFSAANAGDVNNDGKNDIIIGAPFADPGGS
                     ADAGAAYVFSGTTGARLFKFTGPQAGVWFGNSVAGLGDITGDGKSEFAVGAPFTGTDS
                     GAVFVYSGTGTLLYIKKGQAGDQLGWSLAKAGDIDGDGKADLIAGAPFSDIGVTNGGG
                     AFVLSGPTGNVIRQLPGLVTNGFAGYSVCGLADFDGDAIPDLAVGAILDPTGGVFNAG
                     RVSVYSGATGELLYERIGDAVIRELGYSVADAGDLNADGKSELLTGTPSARPNNQPFA
                     GVAFVFTAPPFRGDVTGDAVHDISDIVALVRFVVFGDPVAVPNAADANCDGVTDIVDI
                     VGLVQFVVFGSPKPCCL"
     gene            complement(6351..6686)
                     /locus_tag="AC16GM_10140"
     CDS             complement(6351..6686)
                     /locus_tag="AC16GM_10140"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR2924066.1"
                     /translation="MKRNIRVVDGREILEKVTQLSIKRWSFESQDPQIEHIGPMAQDF
                     YALFGVGDDDKTISTIDPAGIALAAIQELHKENEALKARVSELERLVQTLLTERQDKS
                     GDYTLAAGR"
     gene            complement(6746..8692)
                     /locus_tag="AC16GM_10145"
     CDS             complement(6746..8692)
                     /locus_tag="AC16GM_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924067.1"
                     /translation="MRVPKLLLTATLSLLALPAIYGERSRAIAAVPQLINFQGKLTDV
                     TGNPVADGVYTLTTKIYDDSTAGTVLWTEVRPVQVQGGLFTVQLGSSVALSGNLFSSG
                     TNRFLGVTVNFDPEIAPRTRLISVPYAYQALRADTAITASVALSVAGGPFLPLAGGTM
                     TGPITNTGGPSITMGKANFGVGNNNGGINAFVAGANNMASGNNSAVVGGLNNAASGDV
                     STVGGGTGNTAGGDGSTVGGGVSNSASGYAATVAGGQVNTASGAARATVGGGYSNTAS
                     GTQATVSGGVLNMASGQSATIGGGQSDTASAFGVTISGGNSNLASAFAATIAGGQYNK
                     ARGQFSVVGGGGGFALADSNAALAEASAIGGGKRNIVSGDYAVIGGGRLNFARGQFSV
                     VNGGGGGLVFASDSNSALGDWSVIGGGAANVASGQFSTVGGGTFNTASNNRATVSGGH
                     FNVASGGFAASVGGGERDTSSGSWSVVPGGFGNNAAGDYSFAAGQRAKAVHAGSFVWA
                     DNNNIDFASTSVNQFLIRAAAVGIGTNSPAAKLDVNGSVAMAGPVSIGGGAQISSVLS
                     ASATLNFPSTPTQTSSDLTITVTGASPGDAVSLGVPNASVLADSHFSAWVSAANTVTV
                     RFNNYSATTQDPASGIFSAAVIRF"
     gene            complement(8811..9860)
                     /locus_tag="AC16GM_10150"
     CDS             complement(8811..9860)
                     /locus_tag="AC16GM_10150"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylasparaginase"
                     /protein_id="HIR2924068.1"
                     /translation="MSPNLVEEDKTLTRRNFLTGVSALVGSAAIAEVGLAELLAEKTH
                     PPRRVTSKVVKVRLPVIISTWPQGLAATKIAGELLIAGKSGLDAVEKGINTAEDDPKV
                     QSVGLGGLPDENGIVTLDASIMDGPRRTGGAVGGLENIRNAISVARKVLEDTDHVLLV
                     GDGAKQFAIACGFKEENLLTEESRKAWLEWKKSGQKGFWGRPPDQFGHDTIGLILCDA
                     NHNLYGGVSTSGLSWKIHGRVGDSPILGAGLFVDNDVGAACATGRGEEVIKVCGSFLI
                     VELMRQGHSPQEACEEALRRIRRNNPKRTDFAEAFLAVNKDGETGAAYFGKEFTYAIF
                     APDVHEMRSGEKWEH"
BASE COUNT         1975 a         2931 c         2994 g         2009 t
ORIGIN      
        1 gcgcgcctgg tagtcgccgg gctccaattc ctctttggag agccaaccgt ccgggcgctc
       61 gacgcgggcg aaggccagaa gcgcgaggaa gcggaacgat ttgttcgaaa gcaaaacagg
      121 ctggccatca atttctacag tccagcgctg ttttttgggg acgccgacaa gccaaagccc
      181 tgcttttcgc ggattttgat tccctccctg ctccggtacg gctcgcgccg ggtgggattt
      241 ttcttttgcg ggcgcgccag cttgcggccg ttcgagatgg gccacaattc gctccgcgat
      301 tttttgcggg acaacacccc tcaattcctc gacggtggct tgcttgagac cctcaatcgt
      361 tttccagccg gaagcaacca gtttcagcag gtggtcgcgg ccggcggccg ggccgagagc
      421 atcaaaaaac ggcagcaatt cctcccgcgc gccccatttg acccggcgcg aaagcgattt
      481 gagttccggt gacaatttct ggccgggaca gagcaccagc gccatctgcg cggcggcatc
      541 gagaagccat tccagcgttt cggccacctg ctgaatcgtt ccggccggag aaaagaattt
      601 cttctccaga tcgaaggtga ccaccccgtc gatccaatcc gaaagtaaaa gcgccagttt
      661 gagccggacg accatctgac cggaaacgag gggcccggaa tcgggaggga aattcgtttt
      721 ctctttgagc cagaggccgt ggtaataatc ggaggtgcgt tcgtgaaagt tcagagtgac
      781 ggggaggttc gccgcttcct cggttccgca gagaaaatag aaccagtcta aagcgtcaaa
      841 tcgagttttc gaagaacagg cctcttctat cgctttgccg gtggccaccc gaatgccggt
      901 ctgggccact tttttgccaa aagaggtggc cgcaatgctg ccgttgtcgt gctgggtcaa
      961 aagaccacgc ccagcaaggt cctcaatcgc gcgcttaata tcttttgggc tgacccctcc
     1021 ctgccctccc ctcaagggga gggaagaaca aaaaccctcc ctctcctttg aggagagggt
     1081 cggggagagg tcaggggttt tctgaaccca gtggaacgac ttcagcaacg ttgcttccag
     1141 ttcctcgacc gttttgacgc tctctgcggc gaccaaatcg aggacgatgt cgcccagagg
     1201 caggtgcgac aggcgggatg cgattttctc gcgccggccg gtgacgtagt tactccacag
     1261 cacttcctgc tccagaggcg ttttggccag aagcacggcg cggccggaag gggccaagtt
     1321 caaacgtccg gcccgacccg ccatattctc gaactcagcc acagagaggg gcaccaaaac
     1381 gggccgattg gtctgcgggc cgttgcggta cttccagggc tcgacgaaca cggtctgggc
     1441 gggcaggttc acccccaaag ccagagtcgt cgtggcgcag acgattttga tttcgcccgc
     1501 cagaaacgag tgctccacaa tccggcggag cgaaaagggg aggtcggcgt ggtggaaggc
     1561 gacgcctctg cgcaagcaat aggcgagcga ctcggaaagc accacctccg gctcggcttc
     1621 gatctcttct aacgcacccg ccgcaggcgg ccagggggcg gcttcggcca gaagctgcgc
     1681 taagccctgg caatctcttt tgcctttgag gaagaccaag accgggtcgc cccccgccac
     1741 gcacttttga atctgcgtca ccagccgcgc ccgctggtcg gcgtcaattt tgtcctctcc
     1801 tgcggcactt tccacctccc actgttcctc gccttcttct ttggtattga aggcgcggaa
     1861 ggcaaagcgg ctctcgagca agacgccggt aaagagttcc accgggcgct tgcgctccac
     1921 gagcagacgg cagccgagcc agtcggccaa aggtgcggca tcttccaaaa ccgccgagag
     1981 ggcgacgatc tgcggtctct tcgcaaagga cagaactttc aaaagcgcta attccaagac
     2041 ggggccgcgc gtggggtcgt ccagcatttg aatctcatcg atcaccagaa ggtcgatgag
     2101 ggagaggaga tcaaggttgc ggatcaataa ctggttgaat ttttcgtaga tgaggatggc
     2161 gaggtcgaac tgcccgcgct caatggggcc gtcgaacgcc cggcggtctt tggtggagac
     2221 gacgactttg agtccgagcg gggcgtactt ctgggagaag tcgaggaact tctcttcggc
     2281 gatggctttc aagggaacca gaaagaccgc tttcttccgg cgggccaccg ctgcagccag
     2341 ggccaattca ccgcaaaaag ttttgcccga agaagtcgga gcggcgatca agagactctg
     2401 gccggtgagg atgccggaat caacggcgga gacctggagg ggcaagaggg tttgaccctg
     2461 atgccggttc caggattcca ccagggcgga tggaaggccc attcctctga gttgttcaat
     2521 acgcatgaga aaagttggtg tcggggtgtc gaggtttcgg ggtcaaggaa ttcgttcttt
     2581 gattcgtgac agcgcggcga gggcgccgcg cccacgcgaa agcaagttct gccgcgtagg
     2641 gcgaccgcct cggtcgcctc tggcgttcgc tgcaacctcg acaaccccga cacttcgata
     2701 cttcgacact aacttgcctc ttacgggttc tcaacgcagt agttttttcc acacgccagg
     2761 cagaaaaaga ggcccggggc ggcgacgagt tcttcgttac actccgggca ggtggggagc
     2821 gtctcgggtc tgtttgccac ttccggatgc agatgccgca ggagaagcga gtcatcaatc
     2881 tcgtcctttt cgtcctcttc gatttcaacg ggacgcgggt cgatacgact ttcgcttgcg
     2941 ctcaatttta cgcagcaaga gccgggcatt ttttcctcct cgttttagcg tcaggcagga
     3001 tgcccatacg ggtcgtaccg acctgaccta tggaatgatg gaatttttcc gggcggcctg
     3061 tacgggtcgc ccctacgcaa acacgggcgg gggaaacccc gcccctacgc gaaacaataa
     3121 ccggcaccag ggttttcctg tgttccgtgt tctgtctact gtgtttcgtc tttctcattc
     3181 ctgcgactcc atgcgccaca gccgccagca aaagtcggtg gtgtcccagg aaagttcgaa
     3241 gaaattcagc ccgtcggtca ggtggaacat ccagcggggg aaggtcaacc cgttctcttc
     3301 ccgccacgag cgtagcacct gttggaacgg gaagaccctg ccgttgtaac gcatccagag
     3361 gggcgtcacc ctgctcccga cgacgatggc cttcacctgc acggtgcgat gaagaatggg
     3421 agatagtgtc ggggtgtcgc ggttcgacaa gctcaccgcc ccgagcgaag tcgagggggg
     3481 gtgtcgaggt gtcgtggttc gatccgcctt ggcggactca ccaccctgtc ttcgaccctg
     3541 agggtcttca gacccgatgg gagcttgtcg aagggaagtt aaccccgata ctccgacact
     3601 ccgacacttc gacacgcctt tgggcaatgc ctcctgccag gggttccccc agccgaagac
     3661 cggggcttca ataccgcggg tcattttgat gccttttcca agcacgcagc cattatactg
     3721 ttcaaatgtt cagttgtcaa gtgttttttt aaggtttcgt caaaaaaatt cagggaactt
     3781 agcggggtcg taagtggtta agagtcaata gagaagttcg gcctacggaa cccgaacgtg
     3841 cgggaggatt aacaagtgac ggacttttgc ggggaaggga gcggttgcct caggaggtaa
     3901 ttatgcgcaa ggggaaactg attatttcaa tctgcctttt ggcggtgttt tgcgccggtg
     3961 cggttcaggc gggtgacctg ggtattgggg taaaagttgt aaccgggtcg cactccatcc
     4021 aggtcacgaa tttgacgctt accataccgt tcgtcggtac agtcgcaatg tcgcctctgc
     4081 ccccggccaa agggccgaac gattcgtcga cagtcaccta ttcgctggcg cagtacgggt
     4141 acaatccgac cggcgccagt gtctcgttcc tgatggacgg cgtgccgcac agcgggtcgt
     4201 acagcccgct ccgctacgat acgctctaca cgctcccgtt ccacggcctc ccggattcca
     4261 aaatcagttt ccacctctgc agtctgcgcg gggacgtgaa caacgacttc tacaccgaca
     4321 tctccgacat cgtggcgtcc atacagtacg tggtattcgg caccgcgttg cccgacctga
     4381 cggcgggcga cgtcaacggc gacggcgtcg tggacattac cgacatcgtg gggctggtgc
     4441 agtacgtcgt tttcggcggg ccggtgccga tttgcctctg aaaaacggag attgggtcga
     4501 tacgaccacg ccagtggcgg gggatcagag ttcagggatt agtgaggaat tcttaatgac
     4561 ctcgcctgct ggctggcggt aagcccgtac gggtccatac ggatccgtag gatcgtaccg
     4621 actgttcctt cacagacccc gggcgaggta acctcgcccc tacgttatac aatgccagcc
     4681 ggtgtttaca aacagcaggg cttggggctg ccgaagacga cgaactgaac caggcccacg
     4741 atatctacaa tgtcggtgac gccgtcgcag ttggcgtcag cggcattcgg gacggcgacc
     4801 ggatcgccga agacgacgaa gcggacgagg gcaacgatgt cggagatgtc atgcactgcg
     4861 tcgccggtca cgtcgccgcg aaagggggga gcggtaaaga caaaggccac acccgcgaag
     4921 ggttggttat tgggccgcgc ggagggagtg ccggtcaaaa gttccgattt gccgtcggcg
     4981 ttcaagtctc ccgcatcggc caccgaataa cccagttcgc ggataaccgc gtcgccaatg
     5041 cgctcgtaga gcagttcgcc ggtggcgccc gagtaaacgg agacccggcc ggcgttgaag
     5101 acgcccccag tcggatccag gatcgctccc acagccagat cgggaatggc atctccatcg
     5161 aaatcggcaa ggccgcacac ggaatagccc gcaaagccgt tggtcaccag gcccgggagt
     5221 tgacggataa catttccggt cgggccggag aggacgaagg cgccgccgcc gttggttacg
     5281 ccgatgtcgg agaaaggtgc tccggcgatg aggtcggctt tgccgtcgcc gtcgatgtct
     5341 cccgctttcg ctaaagacca gcccagctgg tcgccggctt gacctttttt gatgtagagg
     5401 agcgtgccgg tacccgaata gacgaagacg gcgccggagt ctgtgccggt aaagggtgcg
     5461 cccacggcga attcggattt gccgtcaccg gtgatgtcac ccaaacccgc cacactgttg
     5521 ccgaaccaca ctccggcctg cgggccggta aatttgaaga gccgcgcgcc cgtggtgccg
     5581 gaaaaaacgt aggcggcgcc ggcatccgcc gaaccaccgg gatcggcaaa aggcgcaccg
     5641 atgatgatgt catttttccc gtcgttattg acgtcccccg cattggccgc ggaaaaaccg
     5701 aactgggtcg gcaggcccga ttggttatcc gccgaatcgc ccacaaagga acgaatgagc
     5761 gcaccgttca ctccggaata gagtcccgcc gttccggcgc tcaaggcgcc gaacctttcc
     5821 gctgaaggcg cggcggccac aaagtcgggc ttgccgtcgc cattgaggtc gcctaccgcc
     5881 gccagagtgg agccgaattc attgccggta tccgcacctg ccacctggta caatgggagg
     5941 ttcgtggcgc cggagaagac gattacctcg cccgcttcgg caatgccgtt ggggtcagcc
     6001 gcgtggacgc cgatgataaa gtccggaaca ccgtcgttgt tcacatcacc cagcccggaa
     6061 accgcgtggc cccagtgctg gtcggcggtg tcggcctgag agcggtagac ctgctggagg
     6121 gagcaggggg gagtcagcgc tcgtgattcg taattggtaa ttcgtaattg gtcggcgcgg
     6181 gcgggaaggg cgaaaagaaa ggtgagaacc gttgcgaaca ggcacgtcgt cttgagatgc
     6241 acacgctctc ctctggttgg cataaacctc ctcctgcgga cagtctgctt tcacctgtaa
     6301 aacaggagag gaggaggaaa agttccaccc gtttgcaaat ccgctgccag ctaccgtccg
     6361 gcagccaacg tgtaatcgcc ggacttgtcc tgccgctcgg tcaaaagcgt ctgcacgaga
     6421 cgctccaatt cgctcacccg cgctttgagc gcctcgttct ccttgtgcag ttcctgaatg
     6481 gcggccagag cgatgcccgc cggatcgatg gtggaaatgg tcttgtcgtc atcccccacg
     6541 ccgaagaggg cgtagaaatc ctgcgccatc gggccgatgt gctcgatttg cgggtcttgg
     6601 gactcaaaac tccagcgctt gatggagagc tgcgtcacct tttccagtat ttctctgcca
     6661 tccaccaccc gaatgttccg tttcaaattg ctgtccgaaa ccgccaccca ggcagaagcc
     6721 caggtcgctc cttatgttcg actcttcaaa atcttataac cgctgcgctg aagatgccgg
     6781 aagccgggtc ttgtgtcgtt gccgaatagt tgttaaagcg cacggttacc gtgttggcgg
     6841 ccgaaaccca ggccgaaaaa tgcgagtcgg caagcacgct ggcgttcggc acgccgaggg
     6901 aaacggcatc gccaggggat gcgcccgtca cggtaatcgt caaatctgaa ctggtttgcg
     6961 tgggtgtact tggaaaattc agagttgcgc ttgcgctcaa caccgaagaa atctgcgcgc
     7021 cgccgccaat cgacactggt ccggccatag ccacgcttcc gtttacgtcc agcttggccg
     7081 ccgggctgtt ggttccaatg cccacggcag ccgcgcggat taaaaactga ttgacgctag
     7141 tggaagcgaa atcaatgttg ttgttatcgg cccagacgaa ggaaccggcg tgaacggcct
     7201 tggccctctg gccggcagca aaagagtaat caccggcggc attgtttcca aaacctcccg
     7261 ggacgacaga ccatgaccca ctcgaggtgt ctctttctcc gccgccgacg gaggcggcaa
     7321 aaccgccgct ggcaacgttg aagtggccgc cgctgactgt ggcgcggttg ttacttgccg
     7381 tgttgaaggt gccgccgccg acggttgaga attgaccact ggcgacgttg gcggcccctc
     7441 cgccaatgac cgaccagtcc ccaagagcgg agttggaatc tgacgcaaag acgagtccgc
     7501 cgccacctcc attaaccacg gaaaattggc cgcgcgcaaa gttgagtctc ccgccgccga
     7561 tgacggcata gtcgccgctg acgatattcc ttttaccccc gccaatggca gatgcttccg
     7621 ccagtgccgc attggaatca gccagggcga atccgccgcc accgccgaca actgagaact
     7681 ggccccgggc tttgttgtac tgtccgccgg caatggtagc cgcaaaggca ctggccaggt
     7741 tgctgttgcc gccgctgatg gtgacgccaa aagcgctggc ggtatccgac tggccacccc
     7801 caatcgtcgc tgactgcccg ctcgccatgt ttagtacccc accactgacc gtggcctgtg
     7861 tgccgctggc ggtattacta tagccgccgc caaccgtggc ccgcgcggcg ccgctggcgg
     7921 tgttgacctg cccgccggcg accgttgccg cataaccgct ggcgctattg gagactccgc
     7981 cgcccacggt tgagccatca ccgccggcgg tgttgcccgt tccgccgccc acggttgaga
     8041 catcaccgct ggcggcatta ttaaggccgc cgacgaccgc cgagttattc ccgctggcca
     8101 tgttgtttgc tccagccaca aaagcgttta tccccccatt gttgtttccc acaccgaagt
     8161 tggcttttcc catcgttatt gatgggccac cggtgttggt gatgggcccg gtcatggtgc
     8221 cgccggcaag gggcagaaag gggccgccag ccacagagag tgctacactg gcggtgatgg
     8281 ctgtgtcagc gcggagggct tggtaggcgt agggcaccga aatcaagcga gttcgcggag
     8341 cgatttccgg gtcgaaattg acggttacgc cgaggaaacg gttggtaccg ctggaaaaga
     8401 gattaccgga aagcgcgacg cttgagccca gttgcacggt gaagagcccg ccctgcacct
     8461 gcaccgggcg gacttcggtc cagaggaccg ttccggcggt ggagtcgtcg tagattttgg
     8521 tggtgagggt gtagacgccg tcggcaacgg ggttccccgt gacgtcggtc agtttgccct
     8581 ggaagttgat gagctggggt acggcggcaa tggctcgact tcgctcgcca taaatggccg
     8641 gtaatgccag gagactgagg gtcgccgtca aaagcagttt cggtacgcgc atggtaaacc
     8701 tcctctgtgg atcgttggtg accaggtgga tttgaaaaca attctgggcg aacatgaagt
     8761 tcgcccctac aagttccttc gatccgttta atccgttggc aaatccgttg tcagtgctcc
     8821 cacttttccc cgctccgcat ttcgtgaacg tccggagcaa atatagcgta ggtgaattcc
     8881 ttgccaaagt aggccgcgcc ggtttcgccg tctttattga ccgccagaaa cgcctcggca
     8941 aagtcggttc ttttcggatt attccgccgg atacggcgca gggcttcctc gcaggcctct
     9001 tgaggcgaat gcccctggcg catcagttcg acgatcaaaa aactgccgca gactttgatc
     9061 acttcctcgc cccggccggt ggcgcaggcg gctccgacgt cgttatccac aaaaagcccg
     9121 gccccgagga tcggggaatc gcccacccgg ccgtgaattt tccacgaaag cccgctggtg
     9181 gaaacgccgc cgtagaggtt gtggttcgcg tcgcagagaa tcaagccgat ggtgtcgtgt
     9241 ccaaattggt cggggggacg gccccagaag cctttctggc ccgacttttt ccattccagc
     9301 caggccttgc ggctctcctc ggtcaaaaga ttttcctctt tgaagccgca ggcaatcgcg
     9361 aactgttttg cgccgtcacc gaccaggagc acgtggtcgg tatcttccag cactttccgg
     9421 gcgacggaga tcgcattgcg gatattttcc aatccgccga ccgcgccgcc tgtgcgccgg
     9481 gggccgtcca taatcgaggc gtccagcgtg acgatgccat tctcgtccgg caggccgccc
     9541 aggccgacgc tctgtacttt cgggtcatcc tccgccgtgt tgatgccctt ctcgacggca
     9601 tccaacccgc ttttccctgc tatcaggagt tcgcccgcaa tctttgttgc ggccaacccc
     9661 tgcggccagg tggagatgat gaccgggagt ctgactttga ctactttaga cgtgactcta
     9721 cgcggggggt gagttttttc ggccagcaac tccgccaggc cgacctcggc tattgcggca
     9781 gaaccgacca gagccgaaac gccagttaag aagttgcggc gggtgagggt tttgtcttct
     9841 tcgacaagat taggggacat gggaacctcc tctgttttgc gtaaaagtat cctgacagaa
     9901 agggaagtc
//
LOCUS       DBNGZD010000387         2929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1172017, whole genome shotgun sequence.
ACCESSION   DBNGZD010000387 DBNGZD010000000
VERSION     DBNGZD010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1172017"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..635)
                     /locus_tag="AC16GM_10155"
     CDS             complement(135..635)
                     /locus_tag="AC16GM_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015299418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR2924069.1"
                     /translation="MTGLSITLLSKRHLKSVWHALDHAIIRFLSMYSLLLLRVALAAT
                     FIWFGALKIFGVSPVADLVAQTVYWVSPKFFVSFLGVWEVVIGLGLLFAVALRFVLFL
                     FVLQMAGTFLVLVLRPEVAFQSGNPLLLTVTGEFVIKNLVLIAAGMVIGTTVRRNQKI
                     ILNGEA"
     gene            complement(749..2557)
                     /gene="lepA"
                     /locus_tag="AC16GM_10160"
     CDS             complement(749..2557)
                     /gene="lepA"
                     /locus_tag="AC16GM_10160"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR2924070.1"
                     /translation="MNNSGISRIRNFCIIAHIDHGKSTLADRLLEATGTLPPREMREQ
                     VLDNMDLERERGITIKAHAVRMSYRAKNGQTYELNLIDTPGHVDFTYEVSRSLAACEG
                     ALLVVDASQGVEAQTISNLYLALENNLTIIPVINKIDLPAAQIPETKKQFADLLGAGD
                     EEIILASAKEGRGIAEILEAIIARIPPPADTPDAPLRALIFDSTFDPYRGAVAYVRVV
                     DGRIAKGTKIKFFAHERIYEVDEVGYLRLRQVPLDSLNPGEVGYVIANIRNVSDTRVG
                     DTITEALRPAPAPLPGFKHIKPMVFSGLYPSAAENYEQLRDAIEKLKLNDASLLYEPE
                     MSNALGFGFRCGFLGLLHMEIVQERLSREYGLSIIATVPNVEYRVTKTSGEVILVDNP
                     AFLPPPGNLEKIEEPFVVVQIITPAEFIGNVMKLCTERRGNYLTTEYVDKSRASLKYE
                     MPLSEIIFDFYDRLKSTTKGYASLDYEYLDYREGPLVKLDILINGDSVDALSVIAHRD
                     KAYHWGKQLCEKLRQLIPRQMFEVAIQAAIGARVIARETVKALRKNVTAKCYGGDITR
                     KRKLLEKQREGKKRMRQIGQVEIPQEAFLAVLKVER"
BASE COUNT          661 a          806 c          817 g          645 t
ORIGIN      
        1 gggatatagg atttcgggaa aacaccggga ggggcgactt tagtggttca acccgcctat
       61 ggcgggttca ccacggtgag tccgccatag gcggatcgaa ccgtcgcccg gcacactttt
      121 acgctcgact gaagtcaagc ctccccgttg agaataatct tttgattccg acgcaccgtc
      181 gtaccaatca ccatcccggc ggcgatcagc accagatttt tgatgacaaa ttcccccgtc
      241 acagtcaaaa gcagtgggtt gccggattgg aaagcaacct cgggccgcag caccagcacg
      301 agaaatgtac ccgccatctg caagacaaac aaaaacagga caaaacgtaa cgccacggca
      361 aacagaagcc ccagcccgat caccacttcc cagacgccga ggaaagagac aaaaaacttc
      421 ggcgacaccc aataaactgt ttgcgcgacc agatcggcaa ccggacttac gccaaagatt
      481 ttgagtgcgc cgaaccagat gaaggtggcc gccagcgcca cccgcaaaag caacaggctg
      541 tacatagaga gaaatcgaat gatggcgtgg tcaagagcgt gccagaccga tttcaaatgt
      601 cttttggaga gcaaagtgat actcaatccg gtcaatgtcc ccgcattggt ggtactttca
      661 actgcccaaa acacgggagg ggcgacttca gtcgcccact aaacttttaa ttgctcggct
      721 gaaatcgagc ctcccagtta catagatctc accgttctac cttcaacacc gctaagaacg
      781 cctcctgcgg aatctccacc tggccgatct ggcgcatccg cttcttcccc tcgcgctgtt
      841 tctccagcag tttccgcttg cgggtgatgt cgccgccgta gcatttggcg gtgacgttct
      901 tgcgcagggc cttcaccgtc tcccgggcga tcacccgcgc gccgatggcg gcctgaatgg
      961 ccacctcgaa catctggcgc gggatcaact gccggagttt ttcgcacagc tgtttccccc
     1021 agtggtaggc cttgtcccgg tgcgcgatca ccgagagggc gtccaccgag tcgccgttga
     1081 tcaggatgtc caatttcacc agcggccctt cccggtagtc gagatattca tagtcgaggg
     1141 aggcgtaccc cttggtggtg gacttgagcc ggtcgtagaa gtcgaaaatg atctcagaga
     1201 gcggcatctc gtacttcaaa ctggcgcggc ttttgtccac gtactcagtg gtgaggtagt
     1261 tcccccgcct ctccgtgcag agtttcatca cgttcccgat gaactccgcc ggcgtaataa
     1321 tttgcaccac cacgaacggc tcctcgattt tttcgaggtt gcccggcggc ggcagaaaag
     1381 cggggttatc gaccaggatc acctcgccgg aggttttcgt cacccggtac tcgacgttgg
     1441 gcaccgtggc gatgatggag agcccgtact cgcgggagag ccgttcctgc acaatttcca
     1501 tgtgcaaaag ccccaaaaaa ccgcagcgga agccgaaccc cagggcgttg gacatttccg
     1561 gctcatagag caacgaagcg tcgtttaatt tcagtttttc aatggcgtcg cgcagctgtt
     1621 cgtagttttc ggcggcggaa gggtacaagc cggagaagac catcggcttg atgtgcttga
     1681 acccgggcaa aggcgccggc gccgggcgca gggcttccgt gatggtgtcg cccacccgcg
     1741 tgtcggagac gttgcggatg ttggcaatga cgtaaccgac ttcgccgggg ttcagggaat
     1801 ccaggggcac ctgacgcagg cgcagatagc ccacctcatc tacttcgtaa atccgctcat
     1861 gggcgaagaa tttgattttt gtgcctttgg caatccgccc atccaccacc cgcacgtagg
     1921 ccactgctcc gcgatacggg tcgaaggtcg agtcgaaaat caaggcccgc agcggcgcgt
     1981 cgggtgtatc cgccggcggc gggatgcggg cgatgatggc ttcgaggatt tcggcaatgc
     2041 cgcgtccctc tttggcggag gcgagaatga tttcttcgtc tccggcgccc aagaggtcgg
     2101 caaactgctt tttggtttct ggaatctgcg ccgccggcag gtcgatcttg ttgatcaccg
     2161 gaatgatggt gaggttgttc tccagcgcca ggtagaggtt ggaaatggtc tgcgcctcca
     2221 ccccctggga agcatcgacc accaagagcg ccccctcgca ggcggcgagg gaccgcgaga
     2281 cttcgtaggt gaaatcgacg tgccccgggg tgtcgatcaa gttcagttca tacgtctggc
     2341 cgtttttcgc ccggtaggac attcgcaccg cgtgggcttt gatggtgatc ccccgctcgc
     2401 gctccaggtc catattatcc agcacctgct cgcgcatttc ccgcggcggc agggtgccgg
     2461 tggcttccag gagccggtcg gccagggtgg atttgccgtg gtcgatatgg gcaatgatgc
     2521 agaagttgcg gatgcgggaa atgcctgaat tattcattgg tcatgtggat taaaggtaat
     2581 catccttaaa gataaatgtt aaaaaatagt tgtcaacatc cgaatggaaa atcttcagag
     2641 catttataac gtctaaggta tcggataaca ctgctggggc ctagcccact ggtaccgcta
     2701 tcttctacga atgatctgtt taattctttt ggtatgagag ggacaaatag atcttactcg
     2761 tcaggtattt aagggggtca ttaaatttgt ttgacaaata aacaaataga agcctatctt
     2821 tcaggtagag aagatgggaa ttttttgtgg aaacaataaa tatgaatagt tagctacccc
     2881 ttgtctccca ggaaagagat atgaattctg ctgaaacgtt gacaagttt
//
LOCUS       DBNGZD010000388         3465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_684014, whole genome shotgun sequence.
ACCESSION   DBNGZD010000388 DBNGZD010000000
VERSION     DBNGZD010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_684014"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1957)
                     /locus_tag="AC16GM_10165"
     CDS             complement(<1..1957)
                     /locus_tag="AC16GM_10165"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIR2924071.1"
                     /translation="MANGREAMTTTTKVQTGLTLERFFTQEGVHPYDTVTWEKRTALI
                     RGADGKVVFEQKEVEFPSFYSQLATNVVVSKYFRGKLGTPQRETSLKQVISRVVNTIR
                     DWGVKDGYFGTAKDAQIFADELTHLLLYQKAAFNSPVWFNVGIELTPQCSACFILSVE
                     DTMPSILEWIRTEGMIFKGGSGSGVNLSPLRSSKEHLAGGGLASGPVSFMRGADATAG
                     AIKSGGKTRRAAKMVVLNVDHPDVTEFIRCKAVEEQKAWALGNAGFDMSINGEAWKSI
                     QFQNANNSVRVTDGFMQAVESDTPWEMREVRNGKVIETVPARELLREIAECTHLCGDP
                     GLQYDTTINSWHTCPNSGRINASNPCSEYMHLDDSACNLASLNLMKFRTPEGDFNPDA
                     FARAVDVLILAMDIIVDNSSYPTEKITRIAKAFRELGLGYANLGALLMARGIPYDSDE
                     GRAAAAAITAILTGEGYRMSAQISEKMGSFAGFEKNRDAMLNVISKHRLSLDNVNAKL
                     LPEKLFAAAEKSWDQAYTQGKKHGYRNSQATVLAPTGTIAFMMDCDTTGIEPDIALVK
                     FKNLVGGGVMKIVNQTVPEALAYLGYSKDEVEEIVKYVEEKGTIEGAPHLKLEHIPVF
                     DCAFKAPGGHRSIHHLGHLRMMGATQPF"
     gene            complement(1996..3081)
                     /locus_tag="AC16GM_10170"
     CDS             complement(1996..3081)
                     /locus_tag="AC16GM_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924072.1"
                     /translation="MFLFTVLLSTFLLVTPTGDRYPELVMENGNYYLVFEGDKKMKVG
                     ERIMTGYDSNVSEFSPDSLYIAYTQNSGLGFESSGKNIYIFEVKIRKERMILCDWFGC
                     WSLGWLQKGDDLLLLIDQDAAESGIKRLVAYDPRTDLITYDTTFEEQSLSSFVTREWK
                     ENLFSYKNRTPRWRDVSLPLEPCQDLTGVCVSGENQPLFDPRLCTNWSAWMPDLPLDS
                     TDALSYYYVEKVTAFAVSPDHRWLAVSSKSASASANIVQDVKSRAPRYLRVLFGGKAG
                     LPYWSSDSRFVAFANEPRGGRPIVHLFDLEKRNNELVAQAMLPPSATVQNVIFGPDIE
                     ETFVEFRTKNKGITRVRMQPVNSNGKK"
     gene            complement(3176..>3465)
                     /locus_tag="AC16GM_10175"
     CDS             complement(3176..>3465)
                     /locus_tag="AC16GM_10175"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR2924073.1"
                     /translation="FYAEMVEGKIPKEDARYILPGGTVSEIVISANFREWRHICEVRC
                     QPFAHWEIRTVMLAILKILKREAPIAFWDFVVDEEKKQATLLPPEPTNGKE"
BASE COUNT          761 a          929 c          956 g          819 t
ORIGIN      
        1 tgaacggctg ggtggcgccc atcatccgca ggtggcccag atggtgaatc gagcggtgac
       61 cgccgggggc tttgaaggcg cagtcaaaga cagggatgtg ctccagtttc aggtgcggcg
      121 caccctcgat ggtgcccttc tcctccacgt atttgacgat ctcctccacc tcgtctttcg
      181 agtaaccgag ataggcgagc gcttccggca cggtctggtt gacgattttc atcaccccgc
      241 cgccgaccag gtttttgaac ttgaccaggg cgatgtcggg ctcaattccc gtcgtgtcgc
      301 agtccatcat aaaggcgatg gtgccggtgg gggcgaggac ggtcgcctgc gagttgcggt
      361 agccgtgctt tttgccctgc gtgtaggcct gatcccagga tttttccgcg gcggcgaaaa
      421 gtttctccgg caataacttc gcatttacgt tatccagact gaggcggtgc ttggaaatga
      481 cgttcagcat cgcgtcgcgg tttttctcaa aacccgcaaa cgatcccatc ttctcgctga
      541 tttgagccga catccggtac ccttcgccgg tcaaaatggc ggtgatggcg gcggcggcgg
      601 cccggccctc gtcggagtcg tagggaatcc cgcgggccat caaaagcgcg cccagattgg
      661 cgtagcccaa acctaattcg cggaacgcct tcgcgattct ggtaattttc tccgtcgggt
      721 aggaagaatt atcgacgatg atgtccatcg ctaaaattaa gacatcaacc gcgcgggcaa
      781 aggcgtccgg attgaaatcg ccttccgggg tacggaattt catcaggttc aaactcgcca
      841 gattgcaggc ggagtcgtcc agatgcatat actccgagca ggggttggag gcgttgatgc
      901 ggcccgaatt ggggcaggtg tgccaggagt tgatggtggt gtcgtactgc aagccggggt
      961 cgccgcagag gtgggtgcac tcggcgattt cgcgcaggag ttcccgcgcc gggacggtct
     1021 cgatcacttt gccgttgcgc acttcgcgca tttcccaggg cgtgtctgat tccaccgcct
     1081 gcataaagcc atcggtgacg cggaccgagt tattggcatt ctggaactgg attgacttcc
     1141 aggcctcgcc gttgatcgac atatcgaacc cggcattccc caaagcccag gccttttgct
     1201 cttctaccgc tttgcagcgg atgaattcgg tcacgtccgg gtggtcgacg ttcaagacca
     1261 ccattttggc ggcgcggcgg gttttgccgc ccgacttgat cgcgccggcg gtggcgtcgg
     1321 ccccgcgcat aaaggagacc ggcccggagg cgagaccgcc gccagctaag tgctccttgg
     1381 atgaccgcag aggagaaaga ttgacgcccg acccggaacc gcctttgaaa atcattcctt
     1441 cggtcctgat ccattccaaa atcgacggca tggtgtcttc caccgagagg ataaagcaag
     1501 ccgaacactg gggggtcagc tcgatgccga cgttgaacca gaccggggag ttgaaagcgg
     1561 ctttctggta gaggaggaga tgggtcagtt catcggcgaa gatttgagcg tctttggccg
     1621 tgccaaaata tccgtctttc acgccccaat cccgaatcgt attgaccacc cggcttatga
     1681 cctgcttgag tgaagtttcc ctttgaggag tgcctaattt tccccggaag tatttggaaa
     1741 cgacgacgtt ggtcgccaac tgggaataaa acgaagggaa ttcgacctct ttctgctcga
     1801 agaccacctt gccgtccgcc ccgcggatta aagccgtgcg cttctcccag gtgacggtat
     1861 cgtagggatg cacgccttcc tgggtgaaga agcgctcaag ggtcaagccg gtttggactt
     1921 tggtggtagt cgtcattgcc tctcgtccat tagccatttc ttccctcctc tggagttatc
     1981 gacaaatttg tccactcatt tcttaccgtt ggaattcact ggttgcattc tgacgcgggt
     2041 aattccttta tttttggttc taaactcaac aaaagtttct tctatatccg ggccgaagat
     2101 aacattctgt accgttgcgc tgggtggcag cattgcctga gctacgagct cgttatttct
     2161 tttttcaagg tcaaaaagat gcacgatggg tcgcccgccg cggggttcat tggcaaaagc
     2221 gacaaagcga gaatcggaag accagtaagg gagaccggcc ttaccaccga ataatacccg
     2281 caggtagcgc ggggcgcggg attttacgtc ttgcacgatg ttggctgagg ctgaggcgct
     2341 cttggacgag acagcaagcc acctgtgatc gggagagacc gcgaaagcag ttactttttc
     2401 gacatagtag taagaaaggg catcagtact atctaaaggt aaatccggca tccatgccga
     2461 ccagttagta catagacgtg gatcaaacag cggctgattt tcaccactta cgcagacacc
     2521 ggttaaatct tggcagggct ctaatggtag ggaaacatcg cgccagcgcg gggttctatt
     2581 tttgtaggag aagagatttt ccttccattc gcgcgtaacg aaagaagata aactctgttc
     2641 ctcaaaggtc gtgtcatatg tgattaaatc tgtgcgcgga tcgtaggcaa ccaaccgctt
     2701 tatgccactt tcagccgcgt cctgatcaat gagtagaagc aaatcatcac ccttttgtag
     2761 ccagccaagg ctccaacagc cgaaccagtc acagagaatc attcgttctt tgcgaatttt
     2821 cacttcaaaa atgtaaatgt tcttaccgga gctttcaaaa ccaagaccac tgttttgtgt
     2881 gtaagctata tacagactgt ccggtgaaaa ttctgaaaca tttgaatcgt atcctgtcat
     2941 aattctttct ccgaccttca tttttttgtc accttcaaaa accagatagt aattgccatt
     3001 ttccataacc agttccgggt acctgtcacc agttggggtg acgagcaaaa aggtacttaa
     3061 caaaaccgtg aacaaaaaca tggttcatac gccagtttag cgcgacgggg acgtcgcgcc
     3121 tacgcgggtg aatatcgtgg ggctgaagcc ccacctacgc gaaataaaga aagcgtcact
     3181 cctttccatt cgtaggttcg ggcggcagaa gcgtcgcctg tttcttctcc tcgtcaacca
     3241 cgaaatccca gaaggcgatg ggcgcttcgc gcttcaagat tttcagaatc gcaagcatca
     3301 cggtgcggat ctcccagtgg gcaaagggct ggcagcgcac ctcgcagatg tgccgccact
     3361 cgcggaagtt ggcggagatg acaatctccg acaccgtgcc gcccggcagg atgtagcggg
     3421 cgtcctcctt gggtattttg ccctccacca tttcggcgta gaagt
//
LOCUS       DBNGZD010000389         1749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1380312, whole genome shotgun sequence.
ACCESSION   DBNGZD010000389 DBNGZD010000000
VERSION     DBNGZD010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1380312"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..556
                     /locus_tag="AC16GM_10180"
     CDS             341..556
                     /locus_tag="AC16GM_10180"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaI-LiaF-like domain-containing protein"
                     /protein_id="HIR2924074.1"
                     /translation="MANAERKDERRGMITGGLILVGLGFIFLLGSLNLVNWDVLWPGI
                     LIVIGLALLAGGALKCRPPSTPTDKPV"
     gene            600..1316
                     /locus_tag="AC16GM_10185"
     CDS             600..1316
                     /locus_tag="AC16GM_10185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR2924075.1"
                     /translation="MTDQQRVGNGSPQPLPAMKKSTFFLLAFLLVGWPLIVAVPEWLL
                     PVAPQDVVNNKIAELYLPTIAVEWLIFALMLLILRLEKESFGSILWRGLTVQNFAVAV
                     AFFFAAGGVLYGVESLLPALGLKVDRNFSFLVPKTQAERFWWVALAVTAGICEEATYR
                     GYLLTRMRRFAKSWVTPVMWSSLSFGIAHSYQGEGNVLLIILYSIMLSLLFIWRKSIV
                     PGIIAHSILDLAQGLYLSGK"
     gene            1318..>1749
                     /locus_tag="AC16GM_10190"
     CDS             1318..>1749
                     /locus_tag="AC16GM_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924076.1"
                     /translation="MRRIRLLICSISFICCARFTSPAFAQDTTSPFGDRLLDSAFTQA
                     LSYIKLTPEDLTFRSDYVPADSFRLYAVNNLMRKPTEVPAKLRQLAEETLGDYSLLNT
                     LIYKMSPFVDAALPPGYIVGSMEQGVPVRSLKDLLVRQYGGG"
BASE COUNT          345 a          497 c          471 g          436 t
ORIGIN      
        1 gcaaaaaagc gcacccggca cccgccggag aattgttttg ggtgcgtatg atgtcaggat
       61 gatttaatgt aagaagggtg tctgcctcag atgattctca gaaccatgat gtcgggaaac
      121 ccctccccca ccctcccctc aaaggggagg gacagggaga aaccaggggt tttcgtctga
      181 agaactccca tttattggac tttcgcgctt atgcaagctg cctgacaaca cttccccgaa
      241 actttatatt ccccgctgga gtacatcatt ttgggattta ttatgaacaa cctcgacact
      301 tcgataccgc gacaccccga cactttttca ggaggttcgt atggccaacg cggaacgcaa
      361 agacgaacgc agggggatga tcaccggcgg gctcattctg gtcggcctgg ggttcatctt
      421 ccttctgggc agtctcaacc tggtgaactg ggacgtcctc tggccgggaa ttttgatcgt
      481 catcggcctg gcgctcttgg cgggcggtgc cctgaaatgc aggccgccga gcaccccgac
      541 ggataaacca gtctaagtct tctcgcgcag agattgagac cgtaccggga cctctgcctg
      601 tgaccgacca acagcgggtg ggcaacggtt cgccgcagcc gcttccggca atgaagaaga
      661 gcaccttctt cttgctggct tttcttctgg tcggctggcc gctcatcgtc gcggtgccgg
      721 aatggctttt gccggtggca ccgcaggatg tggtcaacaa caaaattgcg gaactctacc
      781 tgccgacgat tgccgtcgaa tggttgatct tcgctttgat gcttttgatc ctgcggctgg
      841 aaaaagaaag cttcggctcc atcctgtgga gggggctcac ggttcagaac ttcgcagttg
      901 ccgtcgcgtt tttctttgcc gccggcggcg tgctctatgg ggtggagtcc cttctgcccg
      961 ctctggggtt gaaggtcgat cgaaactttt ccttcctggt gcccaaaacg caggccgaac
     1021 gtttttggtg ggtggcgctt gcggtcactg ccggcatctg cgaagaagcc acctatcggg
     1081 ggtacctttt gacccgcatg cgccgctttg ccaaatcgtg ggtgacgccg gtgatgtggt
     1141 catcactttc ttttggcatc gcccactcct accagggaga aggaaacgtg ctcctgatta
     1201 ttctttacag cattatgctc tcgcttttgt tcatctggcg gaaatcgatc gtcccgggca
     1261 tcatcgccca ctcgattctt gacttggccc aaggattgta tctgtcggga aagtaaaatg
     1321 cgccgcatcc gtctcctcat ctgctccatc agcttcatct gctgtgctcg ttttacctct
     1381 cctgcattcg cccaggacac gacctcgccc ttcggcgacc gccttttgga ctccgccttc
     1441 acgcaggccc tctcctacat caaactgacg ccggaggatt tgaccttccg atctgactac
     1501 gtgccggcgg attcgttccg gctgtatgct gtcaacaacc tgatgcggaa acctacggaa
     1561 gtgcccgcga aacttcgaca attggctgag gaaactcttg gcgattactc gctgctaaac
     1621 accttaattt ataaaatgag tccttttgtc gacgcagctc tgccccccgg ttatatagta
     1681 gggagcatgg aacaaggagt accggtccgt tccttaaagg acttacttgt acggcagtat
     1741 ggaggtggc
//
LOCUS       DBNGZD010000390         3386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_548309, whole genome shotgun sequence.
ACCESSION   DBNGZD010000390 DBNGZD010000000
VERSION     DBNGZD010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_548309"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="AC16GM_10195"
     CDS             complement(<1..459)
                     /locus_tag="AC16GM_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR2924077.1"
                     /translation="MKLLILFLLVLFLSAQSGNASPIVLNEILANEPGSATSLEWIEL
                     FNPGSTPATLDSMVLVVGPDTTRIPDGILLLPISFTVIARKPLSDPPGSASFEQHWGN
                     NSGVWGDTPEENFPLIQGKFSLANGADTVRLYSGSQLIDVFGWRHDAGDGI"
     gene            complement(631..2277)
                     /locus_tag="AC16GM_10200"
     CDS             complement(631..2277)
                     /locus_tag="AC16GM_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924078.1"
                     /translation="MASACGRRFWFALLLLLLPHPASAQLAPFSLGYFFSEDELTEAF
                     RSGDLSFDDYLNLLQFIQERDQLAPSDTLLTDQIYGDRPSSVVPPANFSELKRGQLYF
                     PIEPAPERQLFGSFRSVYTDQVTLSGRQETYTNGRFQYGDEWKGNLSFQKFSSQQFVF
                     RRRSLTRTFGQGGVTVGNFEQKFGNGLLFGHHWRILDKTLLPDDDFFTLGYPTYTRYN
                     GLLFYWQPKNQARTFSLFVSTDRNANASEQLIGSTLERKFRHWTYGVGHGINRTKNAP
                     TGRSIAFSASSIYASYGREKISIQAEAAQVYQHGNGAAIEAAFEPDKDKWVFVSAWNY
                     GKNFLWPHSGGRASSDYLNVTFAENDFEYRSPQVGENGFLLKSLVQLTPRFSSEFKYT
                     NWNQFDFSGRRYRLYGALNYNDRAHFVTGYLRSDSKKASPDSLLRQRLGAEWSYQPNT
                     RLATYLNGELRGDNDALSAASEQVRIAYRLTRPLTLTGWFKYFRQWRGRNFDYWQGYI
                     AEMLELNHSLRVGAKFLADFNRTRTHERTDFFRVEVEGRF"
     gene            complement(2603..2812)
                     /locus_tag="AC16GM_10205"
     CDS             complement(2603..2812)
                     /locus_tag="AC16GM_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924079.1"
                     /translation="MRFASLIAAVLLGLISVGHLLRLIFGVEMVIEGVTLPHWASIPG
                     FIIPGAIALLLVRQERRAKRGPAAG"
     gene            complement(2864..>3386)
                     /locus_tag="AC16GM_10210"
     CDS             complement(2864..>3386)
                     /locus_tag="AC16GM_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924080.1"
                     /translation="KEDEEQYRQNFPKVVITSLQNFTGPAKEKLHERYLGSIRKLSGI
                     APILAFELSGNQNLDLYLEHFDKLLNEFSPLPSDAKSESESASAMEYFRTHVKKLILS
                     DTVHDLENDLRRLARKCGTKVWYQTVRKIHRIRKREKDLPIKFADEAIDKVISPLIKK
                     TEEIIPSSFFQND"
BASE COUNT          818 a          837 c          941 g          790 t
ORIGIN      
        1 aatgccgtca ccggcgtcgt gacgccagcc gaagacgtcg atcagctggg aaccagaata
       61 aaggcgcaca gtgtccgcgc cgttggcaag agagaacttc ccctggatga gcggaaaatt
      121 ttcttccggt gtgtcgcccc agacgccgga gttgttgccc cagtgctgtt cgaatgaggc
      181 gctgccgggc gggtccgaga gcggcttgcg ggcaatgacg gtgaaggata taggcaaaag
      241 aaggatcccg tcgggaatac gggtcgtatc cggcccgacg acgagcacca tgctgtcgag
      301 cgtggcgggg gtgcttccgg gattgaagag ttcaatccat tcgagggaag tggctgagcc
      361 cggttcgttg gcgaggattt cgttgaggac aatgggcgag gcattcccgc tttgggcaga
      421 aagaaagagt acaagaagaa acagtattag caatttcacc tatgacctca ccctaccctc
      481 tcctacagga gagggaaaga taagactatt ctctaataaa aagaaagaga gcaaggaatt
      541 ttccctcccc tttgagggga gggcggggga ggggtcattc cttacttcac caatctgctc
      601 catccgcttc atctgctgtg aaattactcc tcaaaacctt ccctcgacct ctacccgaaa
      661 gaaatccgtc ctctcatgtg ttcgcgtccg gttgaagtcg gctaagaatt tggcgccgac
      721 gcgcagagag tgattgagtt ccaacatttc ggcgatatag ccctgccagt aatcgaaatt
      781 tctcccccgc cactggcgaa aatacttgaa ccagcccgta agggtcaacg gccgggttaa
      841 gcggtaagca atccgtacct gctcgcttgc ggcggacagc gcatcgttat ctccccgcag
      901 ttcgccatta aggtaagtgg ccaggcgcgt atttggttga tagctccact ccgcgccgag
      961 gcgttggcgc aggagcgaat caggtgaggc tttcttcgaa tcactccgca ggtagccggt
     1021 gacgaaatgt gctcgatcat tgtaattcaa tgcgccgtag aggcgatagc ggcggcctga
     1081 gaaatcgaac tgattccagt tggtgtattt gaactcgctg ctgaaacgcg gggtcagttg
     1141 gacgagcgac ttgagcaaaa aaccgttctc cccgacctga ggcgagcggt attcgaagtc
     1201 gttctcggcg aaggtgacat tgaggtaatc ggaactggcg cggccaccgg agtggggcca
     1261 gaggaaattc tttccgtagt tccaggcgga tacgaaaacc catttgtcct tatccggttc
     1321 gaaggcggcc tcgatagccg ctccgtttcc gtgctggtaa acctgcgccg cctctgcctg
     1381 aattgagatt ttctcgcgcc cgtaggacgc atatatgctc gacgctgaaa aagcaataga
     1441 acgcccggtt ggtgcattct tcgttcgatt gatcccgtgt ccaacaccat aagtccagtg
     1501 tcgaaactta cgttcaagcg tgctgccaat cagttgttcg gaggcattag cattgcgatc
     1561 ggtggagaca aaaagactga acgtccgtgc ttggtttttg ggctgccaat agaacaaaag
     1621 gccgttgtag cgggtgtagg tggggtagcc cagtgtaaag aaatcgtcat cgggcaggag
     1681 ggttttgtcc aaaatgcgcc agtggtggcc gaagaggaga ccgttgccga atttctgctc
     1741 gaagttgccg acggtgacgc cgccttgccc aaacgtgcgc gtcaaagacc ggcggcgaaa
     1801 gacgaattgc tggctcgaga atttttgaaa cgacagattc cctttccatt catcaccgta
     1861 ctgaaacctg ccgttggtgt aggtttcctg ccgaccggag agggtcacct gatcggtgta
     1921 gaccgagcgg aatgagccga agagttggcg ttctggtgca ggctcgattg gaaagtaaag
     1981 ttggcctcgc ttcagttcag agaaatttgc cgggggaact acggaagaag gacgatcccc
     2041 gtaaatctgg tcagttagga gagtgtccga cggcgctaac tggtctcttt cctgaatgaa
     2101 ctgcaaaaga ttcaggtagt cgtcaaagga gaggtcgccg gagcggaagg cctcggtcag
     2161 ttcgtcttcg gagaagaagt aaccgaggga aaagggtgcg agctgggcgg aggcggggtg
     2221 tggcaggaga agaagcaaaa gcgcaaacca aaaccgcctg ccgcaggcag aggccattag
     2281 gccaatttta cgtcttactc atttcaggcg caagcgcagg ggcggcaaaa gaaaagagac
     2341 gaattttccc tcccctatga gaggagggat acagggaggg gtcagattta gcactctttg
     2401 acctcacccc gcctgaggcg cgtcttcgac ctcccctctc cccttcgaca tgctcagggt
     2461 ggtgagcccg tacgggtcga accactacga ggagagggaa agataagact aacctcccat
     2521 aaagatggat tttcccctcc cctgtagggg agggatacag ggaggggtca tctctctcac
     2581 ccataacctg cttctaagcc gctcagccgg cggccgggcc ccgttttgcc cgccgctcct
     2641 gccgcaccaa gaggagcgca atagcgcccg gtataataaa gccgggtatg cttgcccaat
     2701 gtggaagcgt taccccttcg attaccattt ccacgccgaa tatcaagcga aggagatggc
     2761 cgacggagat gaggcccaga agaactgcgg cgatcagaga agcgaagcgc atagtaacct
     2821 cctgaagtgg gctttactac gttctatgat atgtggttta cccctagtca ttttgaaaaa
     2881 aagatgacgg aataatctct tcagttttct taatcagtgg ggatatgact ttatctattg
     2941 cttcatccgc aaactttatt ggtaaatcct tctcgcgctt tctaatccgg tgtattttcc
     3001 gcacagtttg gtaccacact ttggttccac atttccgtgc cagtcgccgt aagtcatttt
     3061 ctaaatcgtg cactgtatca ctaaggataa gttttttaac gtgtgttcga aagtactcca
     3121 ttgcagaagc actctcactt tcacttttgg catcagatgg caatgggcta aattcattta
     3181 gaagtttgtc gaaatgttcc aaatataaat ccaaattttg atttccgctt aactcaaaag
     3241 caaggatggg agcaataccg ctaagctttc gtattgatcc aagatatcgc tcatgtaatt
     3301 tctcttttgc aggtccagta aaattctgca atgaggtaat tactactttg ggaaaatttt
     3361 gtcgatattg ttcttcgtct tccttg
//
LOCUS       DBNGZD010000391         1638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_130801, whole genome shotgun sequence.
ACCESSION   DBNGZD010000391 DBNGZD010000000
VERSION     DBNGZD010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_130801"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1638
                     /locus_tag="AC16GM_10215"
     CDS             <1..>1638
                     /locus_tag="AC16GM_10215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR2924081.1"
                     /translation="RLLIFVLLFGIVLALLRQPPQLITPLLGYGFCTLVFLGIVTLHR
                     RRSMPMVLRLVLAVQIVWEVFLEAGIVHQSGNLSQASPYNALFLLTIVSASLVYRLIG
                     TLAIATAASLFYSFAIWGTNPATLPWPWDFSLRQILAATGDDSFAVIFVHICVFYITA
                     FISGYLAERLQHKGEELAETSEQLQKARLDTDDILHHLYSGLLTIDRAGKVIYFNQAA
                     QEILGYRSPEIQGRDFRRVFGHRMPDLVERLQQVLESGRMEPRFELVIVSRSGRQCPV
                     GFTLSPLKDPQGNPRGVIGVFQDLTEIKLLEDRIRTSDRLAAVGELSAGIAHEIRNPL
                     AAIAGSVEVLRKELQLTGDEKRLLDLVIRESERLNQILSEFLHFARIRPTLLTAVDLA
                     SVTRETIEALKRQPLFSGGSELGPRRKIEMKLQSDPALAPVWGEKNQIMQILLNLLTN
                     AAEAMTGKGRAITVRLSNPPEADWVAAAIEDEGCGMTPEQKEKIFEPFYTTKAHGTGL
                     GLSIVHRLVTNLHGRLEVESAPGRGTTVTVYFQKYEKS"
BASE COUNT          311 a          527 c          461 g          339 t
ORIGIN      
        1 cggcttttga ttttcgtttt gctgtttgga attgtcttgg ccctcctgcg ccagcccccg
       61 caattaataa ccccgcttct cggttacggc ttttgcacgc tggtcttttt gggcatcgtc
      121 acacttcacc ggcggcgtag catgccgatg gtattgcgtt tggtcttagc ggtgcaaatc
      181 gtatgggaag ttttcctgga agccggtatc gtccaccagt cgggcaacct gtctcaggcg
      241 agcccgtaca atgcgctctt tttgctcacc atcgtctccg ccagtttggt ctaccgcctg
      301 atcggcacgc tcgccatcgc cacggcggcg agtcttttct attccttcgc catctggggc
      361 accaacccgg cgaccctgcc ctggccctgg gacttttccc tgcggcaaat cctggcggcc
      421 accggcgacg actccttcgc ggtaatcttc gtgcacatct gcgttttcta catcaccgcc
      481 ttcatcagcg gctacctggc cgaaaggctg cagcacaagg gcgaagaact ggcggaaacc
      541 tccgaacaac tccagaaggc ccggctggat accgacgaca tcctccacca cctctactcc
      601 ggacttttga ccatcgaccg cgcaggtaag gtgatctact tcaaccaggc cgcccaggaa
      661 attctggggt accggtcacc cgaaattcag ggccgcgact tcaggcgggt cttcggccac
      721 cggatgcccg atctggtgga gcggctccag caggtgctgg aatcgggccg gatggaaccc
      781 cggttcgaac tggttattgt cagccgctcc ggccgccagt gcccggtggg cttcaccctg
      841 tcgcctttga aagacccgca gggcaacccg cgcggggtca tcggtgtttt tcaggatttg
      901 accgaaatca aactgctgga agaccgcatc cgcacttccg accggctggc cgcggtgggc
      961 gaactctccg ccggcatcgc tcacgaaatc cggaaccctc tggccgccat cgccggctcg
     1021 gtggaggttt tgcggaaaga actccaactc accggcgacg aaaagcggct cctcgacctg
     1081 gtcatccgcg aatcggagcg tttgaatcag atcctctccg agttcctcca cttcgcccgt
     1141 atccgcccga cgcttttgac ggccgttgac ctcgcctccg tgacccgcga aaccattgaa
     1201 gctctcaagc gtcagccgct tttttcgggt ggaagcgagt tgggcccccg ccgcaaaatt
     1261 gagatgaaac tgcagtctga tccggctctg gccccggtct ggggcgaaaa aaatcagatt
     1321 atgcagattc tcttgaattt gctcaccaac gccgcggaag cgatgaccgg aaaaggccgg
     1381 gccattaccg tacggctgag caatccgccc gaggcggact gggtggcggc ggcgatagag
     1441 gatgaggggt gcgggatgac ccccgagcag aaagaaaaaa tcttcgagcc cttctacacc
     1501 acgaaagcgc acggcaccgg cctggggctc tccatcgtcc accggctggt aaccaacctc
     1561 cacggccggt tggaagtgga gagcgcaccc ggccgcggca ccacggtgac tgtctacttc
     1621 cagaagtatg agaaatcg
//
LOCUS       DBNGZD010000392         2764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_894949, whole genome shotgun sequence.
ACCESSION   DBNGZD010000392 DBNGZD010000000
VERSION     DBNGZD010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_894949"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="AC16GM_10220"
     CDS             complement(<1..107)
                     /locus_tag="AC16GM_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924082.1"
                     /translation="MAETKRPVKYIFVTGGVVSSLGKGIATASLGLLLK"
     gene            complement(100..900)
                     /gene="kdsB"
                     /locus_tag="AC16GM_10225"
     CDS             complement(100..900)
                     /gene="kdsB"
                     /locus_tag="AC16GM_10225"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR2924083.1"
                     /translation="MARPAQKKETVWRESTFPKIVAVIPARYGASRLSGKPLVKINGK
                     PLVQHSYEQAKAFRLFADVIVATDDRRVAAAVRKFGGKVQMTDPDHPSGTERVAEVAR
                     KTACDIVVNLQGDELGLAPAYLERLIRPFIDRPVVQMTTLARPISSAREVRNPHQVKV
                     VTDRHGDALYFSRSPLPFPTKGASDHIAPLGHIGVYAYRRDFLLHLCTLPPTPLEKTE
                     RLEQLRVLEHGEKIHVAVVDTDTIRIDTPADLKRARQILEHQSKKRNG"
     gene            complement(905..1192)
                     /gene="gatC"
                     /locus_tag="AC16GM_10230"
     CDS             complement(905..1192)
                     /gene="gatC"
                     /locus_tag="AC16GM_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR2924084.1"
                     /translation="MSITQKDVERIARLARLELSPAEKERYEKDLNTILSYMEQIHDV
                     DTSHVEIIDRVIELPPHLREDVVTPSLPVEAALANAPAREGNFFKVPKVIG"
     gene            1462..2400
                     /locus_tag="AC16GM_10235"
     CDS             1462..2400
                     /locus_tag="AC16GM_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR2924085.1"
                     /translation="MAKTEISAPARDTTYLHPATDFGIRFHYRPPKTVASRRLTIVML
                     LTFLMMVGEIIGGFLTHSLALLSDASHMLTHLAALGIALAAIKISQRPISTQKTYGYY
                     RFEILGAFVNGLTLFAIIAYILYEAVKKLITPQPIEAFAMFIVATAGLAVNLGSAFLL
                     RTSRKDDLNVQGAWFHLLSDTFSSVAIIIGGIVILKTNWYWIDPVLSVVIAVVIAIWG
                     IGLLRESTRVLLEATPSHVDIPAIAEAIREVPGVRAVHDIHVWALSSNMYAMTGHITV
                     DNIPVAETQEILANVNRMLEERFKIGHTNFQFEAIL"
     gene            2420..>2764
                     /locus_tag="AC16GM_10240"
     CDS             2420..>2764
                     /locus_tag="AC16GM_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924086.1"
                     /translation="MDDFEVFLKNLKDSLTAKQKQLLNLVYQNFKISAEWPKSFRIAV
                     DFRKNGDLYKIAEQLGMSLISVGQRYYHKDDESHLTLLGIYLCEGSEEDLRNFTEFAK
                     IAAKKFISNPENA"
BASE COUNT          592 a          777 c          766 g          629 t
ORIGIN      
        1 tgcttgagca aaagtcccag cgaagcggtg gcgatccctt tccccagcga agaaacgacc
       61 ccgccggtca caaatatgta tttcaccggt cttttcgttt cagccatttc tttttttgct
      121 ctggtgctcc aggatttgcc gtgcgcgctt gaggtcggcg ggcgtgtcga tgcggatggt
      181 atcagtatcc acgacggcga cgtgaatctt ctccccgtgc tccaagacgc gcaactgttc
      241 caggcgctca gttttttcca aaggcgtggg cggcagggtg cagaggtgga gcagaaagtc
      301 tcggcggtaa gcgtaaacgc caatgtgtcc taacggggca atgtgatcgc tcgctccttt
      361 ggtcggaaag ggcaaaggcg accgagagaa gtagagcgcg tcgccgtgcc ggtcggtcac
      421 gaccttcacc tgatgcggat tgcgcacctc gcgggcggag gaaattggcc gggccagcgt
      481 ggtcatctgc accaccggcc ggtcgatgaa cggccggatg agccgttcca gataggcggg
      541 ggccaagccc aactcatccc cctgcaaatt caccacgatg tcacaggcag ttttccgggc
      601 cacttcggcc acccgttcgg ttccggacgg atggtcgggg tcggtcatct gcactttgcc
      661 gccgaacttg cgcaccgcgg cggccacccg ccggtcgtcg gtggcgacga tcacgtcggc
      721 aaaaagccgg aaggccttgg cctgttcata actgtgctgg accagcggtt tgccgtttat
      781 cttcaccagc ggtttccccg agagccggct ggcaccgtag cgggcaggga tgaccgccac
      841 aatcttcggg aatgtcgatt cccgccagac tgtttctttc ttttgcgccg gacgcgccac
      901 cttttcaccc gatcactttg ggcactttga agaaattccc ctcgcgcgcg ggtgcattgg
      961 ccagggccgc ttccaccggc agcgaagggg taaccacgtc ctcgcgcaga tgcggcggca
     1021 attcgatcac ccggtcgatg atctccacgt gggaggtatc cacgtcgtga atctgctcca
     1081 tatatgacag aatggtgttc aggtctttct cgtaacgttc tttttcggct ggggaaagtt
     1141 ccagacgcgc cagccgagca atgcgctcta cgtctttttg ggtgatgctc atgtcgtttc
     1201 ttccggatac aaagagtgaa tttcgcttga ttccaagata gcccggcttt tgaccttgtc
     1261 aagaatcttt tcgtgcggat tccgtctgaa cgggttaaga gtcgcgtagg gcgacccctc
     1321 cgggccgtat ggccgccctc ggtcgcctac ttgagcgcgg cgagggcgcc gcgcctacgc
     1381 aatatcggga gcagggaaat cccctatgta ccgggcgctc tcttgacgga aaagcgggaa
     1441 agaggttgct tgaatagcga aatggccaag acggaaatct ccgcccccgc gcgggacacc
     1501 acctacctcc acccagccac cgacttcggg atcagatttc actaccgccc ccccaagacg
     1561 gtggcatccc gacggttgac catcgtcatg ctcctgacct tcctcatgat ggtgggggaa
     1621 atcatcgggg gctttttgac ccacagtctg gcgcttttga gcgacgccag ccacatgctg
     1681 acgcatttag cggctttagg cattgcgtta gccgccatca aaatcagcca gcggccgatt
     1741 tccacccaga agacctacgg ctactaccgg ttcgaaatct taggcgcttt cgtcaacggg
     1801 ctcacccttt tcgccatcat cgcttacatc ctctacgagg cggtcaaaaa actcatcacc
     1861 ccgcagccga ttgaggcctt cgcaatgttc atcgtcgcca ccgccgggct ggcggtgaac
     1921 ttaggcagcg cctttttgct ccgcacgagc aggaaggacg acttaaacgt gcaaggcgcc
     1981 tggttccact tgctttccga caccttttca tcggtagcca tcatcatagg cggaattgtc
     2041 attctcaaaa ccaactggta ctggattgat ccggtgcttt cggtggtgat tgcggttgtc
     2101 attgccatct gggggatcgg gctactgcgc gaatcgacgc gggtgctttt ggaggcgacg
     2161 cccagccacg tggacattcc cgccatcgcg gaggcgatcc gggaggtgcc cggggtgcgg
     2221 gcggtgcacg acatccacgt ctgggcgctc tcgtccaata tgtacgccat gaccgggcac
     2281 atcaccgtag acaacatccc ggtggcggag acgcaggaga ttttagccaa tgtcaaccgg
     2341 atgttagagg agaggttcaa aatcgggcat acgaattttc agtttgaagc tatattatag
     2401 tgttgaggaa aaaaccacta tggatgattt tgaagtcttc ctcaaaaact taaaagattc
     2461 tcttacggct aaacaaaaac agttgctcaa ccttgtctac cagaacttca aaatttcggc
     2521 cgaatggcca aaatcgttca gaatcgctgt tgacttccga aaaaatgggg acctgtataa
     2581 gatcgctgaa cagctgggaa tgtccctgat tagcgtaggt caaagatact accacaaaga
     2641 tgatgaaagt catttgactc ttttggggat ttatctgtgc gaaggttcag aagaagactt
     2701 aaggaacttt actgaatttg ctaaaatagc cgcaaaaaaa tttatttcca atcccgaaaa
     2761 tgct
//
LOCUS       DBNGZD010000393         2287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1794931, whole genome shotgun sequence.
ACCESSION   DBNGZD010000393 DBNGZD010000000
VERSION     DBNGZD010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1794931"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16GM_10245"
     CDS             <1..308
                     /locus_tag="AC16GM_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2924087.1"
                     /translation="DLDGDGRAEFIVGAPFADPGGLTAAGTAYVFSFPCVARGDVDLS
                     GVTTIEDAVTLLRYVVLGEALPNPTVADVNCDGAWDIVDLVLLVRYLVFGNPTPCCL"
     gene            426..>2287
                     /locus_tag="AC16GM_10250"
     CDS             426..>2287
                     /locus_tag="AC16GM_10250"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR2924088.1"
                     /translation="MKRAVRFFVTALFLLVGVGVSRGQVIKPDTGQRASSVSDKAEDE
                     RKALLAQFKQTYNVKTLRLDKITGVPRRVEGELSRGLKSREPAEAIYEFLEMNREVFR
                     MRDPRQELILEWVKRDNFGRRRMHVLFKQVHNSVPVLGGTFGGHLSSKGELEVIAGSY
                     YPGIEVSTVPGISVEEAEARALQDILSKKKPRNQYEAEIETTVYKSQLIIYPTPDYLQ
                     TRLKKPYYLVWRVERGRWVYFIDANNGEIYHKLPGFANLQQPSGDALRSDSIESAGLV
                     RGEPTPIRVIPVHSKYDHPSSLLWSTDVRVNINPGDQKAVSLAAATNGDLYAVFEDYN
                     PAFPPVPFLHLDKSTDNGATWTHVWAGGLINYDIHLQPQIVIGEGTQNYIFIFAEGRR
                     INRGDWDVVYLRISLADPSQIVGFSLNSPGLNEVRPRITLRTDVSPYQLHLAFAQVED
                     GIVRVLRSSDFGLTWEGPGLSRLYTDDHRIAYGRNGRLYIALEQLGNVFCHRSVDGGA
                     TWSEIPVILGSGGTKISIAAAHNFSDRMVIVYSKLVGGQRDIYLSTSQDGGNTWTIDR
                     PLFQSSPTEENWAGCAADPAGFGKDDFYVAFYSSGLVKEMYSPDLQNWSSAVTIS"
BASE COUNT          536 a          510 c          672 g          569 t
ORIGIN      
        1 gggatctgga tggagacggg cgggccgaat tcatcgtcgg cgcgccgttt gccgatccgg
       61 gcgggctgac ggcggcgggg acggcttatg tgttttcttt tccgtgcgtc gcgcgcgggg
      121 atgtggattt aagcggagtg accaccatcg aggatgcggt cacgctcctg cgctacgtgg
      181 ttcttgggga ggcgctgccg aatccaacgg tggcggacgt gaactgcgac ggggcgtggg
      241 acattgtcga tttagtgctg ctcgtccggt atctggtgtt cgggaatccg accccgtgct
      301 gtttgtagga cggtaagcgt ttgaggaagt gagcattagc gtttggggga gtttcagatg
      361 agaaattcct tgacaacttc tgaagagatg agagagatta cctgtataca gaggaggtag
      421 gggcaatgaa aagagccgtt cgatttttcg taaccgcgct gtttctcctt gtgggggttg
      481 gggttagcag aggtcaggtc attaaaccgg ataccggaca acgtgcgtcc tctgtttccg
      541 ataaagccga ggacgagcgc aaggcactcc tggcgcaatt taaacagact tataacgtta
      601 agacgttgcg gttggataag ataaccgggg tgccgaggcg ggttgagggt gaactttcac
      661 ggggactcaa aagccgtgaa cctgcggaag cgatatacga atttcttgaa atgaatcgcg
      721 aagtctttcg gatgcgcgat ccgcgccaag aacttatcct ggaatgggtt aagcgagaca
      781 acttcgggag aagaaggatg cacgttctct tcaaacaggt gcacaatagt gttcctgttt
      841 tgggaggtac ttttggcggc catttgagca gtaaggggga acttgaggtt attgcggggt
      901 cgtattatcc gggcattgag gtttcgaccg tgccgggtat tagtgtggaa gaagccgagg
      961 ctcgagcact ccaggacatt ctgtcgaaga aaaagccacg caatcagtat gaagccgaaa
     1021 tagaaacaac agtatacaag tctcaactca ttatctaccc gacccctgat tacctgcaaa
     1081 cacgcttaaa gaagccgtac tacttggtct ggcgggtgga aaggggacgc tgggtgtact
     1141 tcatcgacgc caacaacggt gaaatttacc ataagttacc tgggttcgct aaccttcagc
     1201 agccatcggg cgatgcgctg cggagcgatt caattgaaag cgcgggctta gtgcgagggg
     1261 agccaactcc tatccgggtc attccagtcc attctaagta cgaccatccg agttcattac
     1321 tctggagtac ggatgttcgc gtcaatatta accccggtga tcaaaaagcc gtctccctgg
     1381 cagcagccac caatggcgat ctgtatgcgg tcttcgagga ttataatccg gcgtttcctc
     1441 cggtaccatt tcttcattta gacaaatcta ctgataacgg ggctacctgg acgcacgtgt
     1501 gggctggcgg tctgattaat tacgatatcc atttacagcc acaaatagtc ataggcgaag
     1561 ggacgcaaaa ctacatcttc atctttgcag agggacggcg cataaataga ggtgattggg
     1621 acgtcgtata tttgcgaatt tctctggctg atccaagtca aattgtgggt ttttctctta
     1681 actctccggg gttaaacgag gtgaggcctc gcataacctt acggactgac gttagtccct
     1741 atcagcttca tctggctttt gcgcaggtcg aagatgggat agtccgggtt ttacggtcct
     1801 ctgatttcgg gctcacttgg gagggcccgg gactgagtag attatatacc gatgaccatc
     1861 ggattgcgta cggtagaaat ggtcgcctgt acattgctct tgagcaactc ggaaatgttt
     1921 tctgtcatcg ttcagtagac ggcggcgcca cctggagcga gataccggtg attttgggaa
     1981 gtgggggcac gaaaatatcc attgccgcgg cccacaactt ttcggatcgt atggttatag
     2041 tgtattcaaa gttggtgggc ggtcaacgtg acatttactt aagcacgagc caagatgggg
     2101 gtaatacatg gactatagac aggccgcttt ttcaatcttc tccaaccgaa gaaaactggg
     2161 ccggttgcgc agccgatcca gcgggtttcg ggaaagatga tttttatgtt gccttttatt
     2221 caagtgggct ggtaaaagag atgtactcac ccgacttgca gaactggagt tcagccgtca
     2281 caatctc
//
LOCUS       DBNGZD010000394         4980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1451362, whole genome shotgun sequence.
ACCESSION   DBNGZD010000394 DBNGZD010000000
VERSION     DBNGZD010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1451362"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="AC16GM_10255"
     CDS             <1..1176
                     /locus_tag="AC16GM_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2924089.1"
                     /translation="SFLTILGVFIGVTSVIGMASIIKGLDRSMARQIESLGSNVIYVS
                     KHKPGIVIGNRSEEERNRKGITYDDAVAILERCPAVAAISPENHYYPFPSGNRVKYKN
                     QSARRPDFLGVLPAYEQVNNSFVSEGRFITESDHQYRSLVTVIGQDVADALFGRLDPI
                     GKEILVNNDKFLVIGVVEKRESILGQSQNNFVLIPYGTFAKIHPEEKELWLAVQAKSR
                     ADMPTATDQIRDLMRRRRGVPYDKPDNFAVFTQDDLLQVYNQVTFAIYMVMIVISSIG
                     LLVGGVGVMNIMLVSVTERTREIGIRKAIGAKRNNILYQFLIEAMTLSGTGGILGILF
                     GVLVSQFVAAVSPLPAAVSGFWVFLGFTVAVSVGLIFGIYPAYRAAKVDPIVSLRYE"
     gene            1270..2610
                     /locus_tag="AC16GM_10260"
     CDS             1270..2610
                     /locus_tag="AC16GM_10260"
                     /inference="COORDINATES: protein motif:HMM:NF040439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2924090.1"
                     /translation="MVRFCRLTSFPLLLTVVLFTLTVAPAKAQYGFQSREPLAGQPKF
                     QLDAAAFGLFDEGKVRVEVYYRIPADGLRFAKEKDRYRAEYEMAVVLADKKGRQIDAT
                     TFSEKISVDSYQETQSKGDLLINQLNFTALPGEYKVVATLTDRNSGQTYSQTDRLKAQ
                     PFQGGAVTLSTLEFARTVFDSTEAGRFQKGHRFVLPAVSRLYSDSLPRLVLYYEVYSR
                     EKQPSTFQVDYFAASRSLRKRVIDTATVTAVPGVTPVVASLNVSRLPPGAYTLSAVVK
                     VSKGKEVCHQEGEFIIAWSLASILRNDFFGAVEQLRYIATAAEMKALKRSRSAERLKN
                     WDEFWRKKDPSPGTPENEAAEEYFRRIRYANANFTVGLREGWKTDLGRVYVIYGEPDQ
                     IEVHPFERDAKPYQVWFYYQSTRSLFPRTFVFVDETGFGDYELQYPYDGVIRSF"
     gene            2626..4044
                     /locus_tag="AC16GM_10265"
     CDS             2626..4044
                     /locus_tag="AC16GM_10265"
                     /inference="COORDINATES: protein motif:HMM:NF040439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2924091.1"
                     /translation="MPFCTIGRHRLNALMLVLLALAGAGAARAGDSLSFWVDHACFKH
                     LDDPQKTYVEVYVTIDRRQLTFQPYEGKFKAIGSFFLSITDSTGQQIDSVGFHVASSV
                     ADPAEAQREHFGVPAVFGFVLKPGNYRAALEVVDRLSKKSGERSFPLVVPSFAADSLS
                     LSEIELATKTSAAELGVTDTLVGMFQKNGLVVIPNPAGTCARQDKKLSFYAEAYGFTQ
                     TQPEYLLQVAVLDTQGLVVKDYGQKAFAKPGSTAVLASSIPIDSLPEGKYLLDLTVVD
                     RATGQVAEQRKRFTVVGAVTLPQTLTFADSGAFREEEAKAAKSLISYLATPEELLQYD
                     RLPLGEKYSFIQNFWKKRDPDPNTRINEFLTEYYRRFLYANANFSADLKEKKDGWKTD
                     LGRVYMVYGPPNDIERGYMASDQAAWERWQYSSLPGQGGVYFLFVEEHDTGRWRLVDS
                     TARREIHDPRWEERIQTERLQP"
     gene            4186..4806
                     /gene="lexA"
                     /locus_tag="AC16GM_10270"
     CDS             4186..4806
                     /gene="lexA"
                     /locus_tag="AC16GM_10270"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR2924092.1"
                     /translation="MEKNVRPLTKRQKQILDFIDSTIQKAGHPPTLREMGKRFGIKST
                     NGVRTFLIALEKKGYIKKAPYRSRGIYMADQLKAAWRPVPVVGKIAAGLPITAIENIE
                     SSIAIDKTFLPGDGVFSLKVTGESMKNAGIFDGDYVLVQQQATANPGDIVVAIIGEEA
                     TVKRFYPEKGHVRLMPENPDFSPLIVEKNTPGFSLAGKVVGVMRRL"
BASE COUNT         1080 a         1412 c         1454 g         1034 t
ORIGIN      
        1 tcgtttctga ccatcctggg cgtcttcatc ggcgtcacct cggtgatcgg catggcgtcg
       61 atcatcaaag ggctggatcg gagcatggcc cggcagatcg agtccttagg ttccaacgtc
      121 atctacgtct ccaaacacaa gccgggaatc gtcatcggca accgatccga agaagagcgc
      181 aaccggaaag ggatcaccta cgacgacgcg gtggccattc tggagcgctg tccggcggtg
      241 gcggcgatct cgccggaaaa tcactactac ccgtttccct ccggcaaccg ggtaaagtac
      301 aaaaaccaga gcgcccggcg ccccgacttt ttgggcgttt tgccggcgta cgagcaggtg
      361 aacaattcgt ttgtctccga ggggcgcttc attaccgaga gtgaccacca gtaccggtcg
      421 ctcgtcaccg tcatcggcca ggacgtggcc gacgccctct tcggccggct cgaccccatc
      481 ggcaaagaga ttctggtcaa caacgacaag tttctggtga tcggggtagt ggagaaacgc
      541 gaaagcatcc tgggccagag tcagaacaac ttcgttttaa tcccctacgg caccttcgcc
      601 aaaattcatc cggaggaaaa agaactctgg ctggcggtgc aggccaaaag ccgcgccgac
      661 atgccgaccg ccaccgatca aatccgcgac ctgatgcgcc gccgccgggg cgtcccctac
      721 gacaagccgg acaacttcgc cgttttcacc caggacgacc tgctccaggt ctacaatcag
      781 gtcaccttcg ccatctatat ggtcatgatc gtcatctcct ccatcggcct tttggtgggc
      841 ggcgtggggg tgatgaacat catgctggtc tcagtcaccg aaaggacccg cgagatcggc
      901 atccgcaagg cgatcggcgc caaacggaac aacatcctct accagttttt gattgaagcg
      961 atgaccctct ccggcaccgg tgggattctg ggcatcctct tcggcgtcct ggtgagccag
     1021 ttcgtggcgg cggtgtcgcc tctgccggcc gcagtttccg gtttctgggt ttttctgggg
     1081 ttcaccgtgg cggtttcggt cgggctgatc ttcggcatct acccggccta ccgggcggcc
     1141 aaggtcgacc cgatcgtgtc gctgcgctac gagtagtttt ttctgcccga aagagtgccc
     1201 gcagccgtat caacaaaggc aatggggaat cttttgctct tttccggtca aagcgtatct
     1261 tttcccgtga tggtgaggtt ctgccgattg acctctttcc ctttgctttt gaccgtggtc
     1321 ctgttcaccc tgaccgtggc accggccaaa gcgcagtatg gctttcagtc cagggaacct
     1381 ctggcgggcc agccgaagtt ccaacttgat gcggcagctt ttgggctctt cgatgaaggc
     1441 aaggtgcgcg tggaggtgta ttaccggatc cccgcggacg ggctgcgttt tgccaaagaa
     1501 aaggaccgct accgggcgga atacgaaatg gctgtggttc tggccgacaa aaaaggcagg
     1561 caaatcgacg ccaccacttt ttcggaaaaa atctccgtcg attcgtacca ggaaacgcag
     1621 tccaaaggcg acctgttaat caaccagttg aacttcaccg ccctccccgg cgaatacaag
     1681 gtggtggcga ctctgaccga ccgcaattcg ggacagacct attcgcagac cgaccggctc
     1741 aaagcgcagc cattccaggg tggtgcggtg accttaagca ctctggaatt cgcccgcacg
     1801 gttttcgact caaccgaagc gggacgattc cagaagggcc accgcttcgt cctgccggcg
     1861 gtgagccggc tctacagcga tagcctgccg cgcctggtgc tctactatga agtttacagc
     1921 cgggagaaac agccgtcgac gtttcaggtc gactatttcg ccgccagccg aagtctgcgg
     1981 aagcgggtca tcgacaccgc gaccgttacc gcagtgcccg gggtgacgcc cgtcgtcgcc
     2041 tcgctgaacg tcagccgcct gccgccgggg gcctataccc tgtcggccgt ggtgaaagtg
     2101 tccaaaggga aagaagtctg tcaccaggag ggtgagttca tcatcgcctg gtctctggcc
     2161 agcatcctgc ggaacgactt cttcggcgcg gtggagcaac tgcgctacat tgccacggcg
     2221 gcggaaatga aggcgctcaa gcggtcgcgc tccgccgaac ggctgaagaa ctgggacgag
     2281 ttctggcgga agaaagaccc gagtcccggc acgccggaaa acgaagcggc ggaggaatat
     2341 ttccgccgca tccgctacgc caacgccaac ttcaccgtgg gcttgcggga ggggtggaag
     2401 accgacttag gccgcgtcta cgtcatctac ggcgagccgg atcagatcga agtgcacccg
     2461 ttcgaacgcg acgccaagcc gtaccaggtc tggttctact accagagcac ccggagtctc
     2521 ttcccgcgca ccttcgtctt tgtggatgag accggtttcg gcgactacga actccagtac
     2581 ccctacgatg gggtgattcg ctccttttaa cgagtgagga tgagcatgcc cttctgcact
     2641 attggccgcc atcggctgaa cgctctgatg ctcgtgcttc tcgcgctggc cggagccggc
     2701 gcggcccgcg ccggggattc gctttcgttt tgggtggatc acgcctgctt taagcactta
     2761 gacgatccgc aaaagacgta cgtcgaagtg tacgtcacca tcgatcggcg ccagttgact
     2821 ttccaacctt acgaggggaa gttcaaagcg ataggctctt tctttttaag cataactgat
     2881 tcgactggcc aacagattga ttcagttggt tttcacgtcg cctcaagcgt tgccgaccca
     2941 gccgaggcgc agcgcgaaca tttcggtgtt ccggcggtgt tcggtttcgt cctgaagccg
     3001 ggaaactatc gggccgcgct ggaggtagtt gaccgtcttt ctaaaaaaag cggggagcgt
     3061 tcttttccgc tcgtcgtccc gagcttcgcc gccgatagcc tctctttgag tgaaattgaa
     3121 ctggcgacga aaacttcggc cgccgaactt ggggtgaccg ataccttggt cgggatgttt
     3181 cagaaaaatg gtttggtggt aataccgaac ccggcgggca cctgcgcgcg gcaggataaa
     3241 aaactttcct tctacgccga ggcctacggt tttacccaga cgcagcctga atacctgctg
     3301 caggtggccg tgctcgacac gcagggctta gtggtaaaag actacggcca gaaagcgttt
     3361 gccaagccgg gttccaccgc cgttttggcc agttccattc cgatcgatag tctgccggag
     3421 ggcaagtatc tcctcgatct caccgtggtg gaccgggcca ccggtcaggt tgccgaacaa
     3481 aggaaacggt tcacggtggt cggtgcggtg accctgccgc agaccctcac attcgccgac
     3541 agcggggctt ttcgagagga ggaagcgaaa gcggcgaaaa gtctcatttc ctatctcgcc
     3601 acgcccgaag agcttttgca gtacgaccgc ctgcccctgg gtgaaaagta ttccttcatt
     3661 caaaatttct ggaaaaagcg cgacccggat ccgaacaccc ggataaacga gttcctgacc
     3721 gagtattacc ggcgcttcct ctatgccaac gccaacttct ccgccgacct gaaagaaaag
     3781 aaagacggct ggaagaccga cctgggccgg gtctacatgg tctacggccc gcccaacgac
     3841 atcgaacggg gctacatggc gagcgaccag gcggcctggg agcgctggca gtactccagc
     3901 ctgccggggc agggcggcgt ctatttcctc tttgtggaag agcatgacac cggtcgctgg
     3961 cgcttagtcg attccaccgc ccggcgggag attcacgacc cgcgctggga agaacgtatc
     4021 cagaccgaac ggctccagcc gtaaggcact ccggaaattc gtaagccacc agagagtgat
     4081 tttttctctt gacagctatt cttttgttca gtatatacgg atgtgtagct tgcccgacgt
     4141 agactttaag gttgtggccg gggataaaaa ggaggttata tggtaatgga gaaaaatgtt
     4201 cggccgttga ccaaaagaca gaagcaaatt ttggatttca ttgacagcac tattcaaaaa
     4261 gccggccatc cgccgacctt gcgggagatg ggcaaacgct tcgggatcaa atcgaccaac
     4321 ggcgtgcgga ctttcctcat cgccctggag aagaaagggt acatcaagaa agcgccctac
     4381 cgctcgcggg ggatttatat ggccgaccaa ttgaaggccg cctggcggcc ggtgccggtg
     4441 gtggggaaga tagcggcagg actgccgatt acggccatcg aaaatatcga atcgagtatc
     4501 gcgatagata aaaccttcct gccgggtgac ggcgtctttt cgctcaaagt caccggcgaa
     4561 agtatgaaaa acgcagggat ttttgacggg gactacgtct tagttcagca gcaggccact
     4621 gccaatccgg gggacatcgt ggtggcgatt atcggggaag aggcgacggt caagcgcttc
     4681 tatccggaga aaggtcacgt ccgcctgatg ccggaaaatc ctgatttttc gccgctcatt
     4741 gtcgagaaga acacgccggg attctccctt gcggggaagg tggtgggggt gatgcggcgg
     4801 ctctaaaaga aaagacagga ttgaggattg aaaactgagg gagagaaaaa gaccgatgaa
     4861 gagacttttt ctaaaagcat gcactttctg tgcttttgac gtagtttagg tagagcacaa
     4921 ggaataatca aaatggggta gacgcaccgt gtccaagccg aagatttttt cagctaatag
//
LOCUS       DBNGZD010000395         3651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1659346, whole genome shotgun sequence.
ACCESSION   DBNGZD010000395 DBNGZD010000000
VERSION     DBNGZD010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1659346"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..609
                     /locus_tag="AC16GM_10275"
     CDS             52..609
                     /locus_tag="AC16GM_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2924093.1"
                     /translation="MKTKQSNFDSAWWGGRAPGLTVVLLGFLFALGTSAFAADQRKAT
                     GSQLSPVQESKPAEARATAPLQIELECLSVQGSGATFVTGGGLQLGFSVAQSVAGAQS
                     AATRKLGIGFWGACAACALLGDLNGDLVRDIVDIVAMINYVVFGDPVATGLICADLTG
                     DGVVDISDIICLINNVVFSNPIPCL"
     gene            complement(682..1731)
                     /locus_tag="AC16GM_10280"
     CDS             complement(682..1731)
                     /locus_tag="AC16GM_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924094.1"
                     /translation="MVGIDSYRNNNLWLKSRAWDLSLLTFSGVLSIFPHALYSWMGFK
                     PWMVGLTVALLVGGPHIYATFTRILLDKSFTQRFSNYIFIVFAVIPVAVVYLLITRQV
                     LLLTIFFFWASMHVLHQIAYVVELYNQKMPVRIPNIRRAAEYFLVFTSLYPFAISKLV
                     SGNFYIGTKKLLFPQFMAGPAIPILVWTVFGLSLLVVIYHSLRDWIRERRIYPKTYLL
                     GVTVVVCGIAPTFPDLDVAFQGVNIWHSFQYLALTYYITRLKKAEGLLREKPVARVIE
                     KRQGLNFYLFHVGITLLAGVAVVALYKFSGLSWQMAYWAVIFSSLLVHYFFDHILFTE
                     FRLFKTRLKAEPALA"
     gene            2156..2293
                     /locus_tag="AC16GM_10285"
     CDS             2156..2293
                     /locus_tag="AC16GM_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924095.1"
                     /translation="MRRHRSPALLFWIAIGLTLLAILLTYSDMDGQTATSTAKSLISL
                     F"
     gene            complement(2415..3194)
                     /locus_tag="AC16GM_10290"
     CDS             complement(2415..3194)
                     /locus_tag="AC16GM_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster loop"
                     /protein_id="HIR2924096.1"
                     /translation="MAQPSIRDILIKRGEEILSQPFRPIVFARHQEADALLNDLDRYP
                     HEFVLACIMDRQIIAEKAWMIPNEFKERLGSFEFTNLQALSLERVQRIMSNPKPLHRF
                     PIVMSKNFFSAVQKIARVYSGKASNIWAGCPDSVTIVNRFREFDGVGPKIGPMAANIL
                     VRDFKIPIGNKNSIDISPDVHVRRVFTRLGLIVEGASNEELIFRARELNPTYPGVFDL
                     PAWEIGREWCKPNKPNCPKCYMNGCCPTARITKGGKTGRNA"
     gene            complement(3351..>3651)
                     /locus_tag="AC16GM_10295"
     CDS             complement(3351..>3651)
                     /locus_tag="AC16GM_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924097.1"
                     /translation="LKREIAEQLGVRKCRGESLDSPKNAGEPKGSPLHATGRERPNMI
                     DIENDRRGHYRLALKPEEIDFDFATLAKHPDVEIKKGATRLSTYCPNKMNATQKE"
BASE COUNT          849 a          920 c         1042 g          840 t
ORIGIN      
        1 cctgcagacg ctggaagagc aggtgaagca gcagaacgcg ggagggggcc gttgaaaacg
       61 aaacagtcga atttcgattc ggcgtggtgg ggcggcagag cgccgggcct gactgtcgtt
      121 ctgctcgggt ttctcttcgc tctcggtact tccgcgttcg ctgccgatca gcggaaggca
      181 accggttcgc aactctcgcc ggtgcaggag agcaagcccg ccgaagcccg cgcaaccgca
      241 ccgctccaga ttgaactgga gtgcctttcg gttcagggaa gcggggcgac cttcgtcacc
      301 ggcggtgggc tgcagttggg gttttcggtg gcccagtcgg tggcaggcgc acagagcgcg
      361 gccacgcgca agctcggcat cgggttctgg ggggcctgcg cggcctgcgc gcttttgggt
      421 gatttgaacg gcgatttggt gcgagacatc gtggacatcg ttgccatgat caattacgtc
      481 gtgttcgggg acccggtggc cacgggtctc atctgcgccg atttgaccgg cgacggcgtg
      541 gtggacatca gcgacatcat ctgcctgatc aacaacgtcg ttttcagcaa cccgatcccc
      601 tgcttgtaga agaaaagagc agcaatcgaa agagcgcccc tgcgggcgct cttttttttc
      661 tgcccaattc gggtgcgcga gctacgccag ggccggctcg gctttgagcc gggttttgaa
      721 cagccggaac tccgtgaata ggatgtggtc gaaaaagtag tgaaccagga gcgaggagaa
      781 aatcaccgcc cagtaggcca tctgccagga gaggccggaa aatttataga gcgccaccac
      841 cgccaccccg gccagaaggg tgatgccgac gtggaagagg tagaagttca acccctggcg
      901 cttctcgatc acgcgggcca ccggtttttc acgcaggagg ccttcggctt tcttgagccg
      961 cgtgatgtaa taggtcaagg ccaggtactg gaacgagtgc cagatgttga cgccctggaa
     1021 ggccacgtcc agatcgggga aagtgggagc gatcccgcag acgacgaccg tcacgcccag
     1081 taagtaggtc ttggggtaga tgcgtctctc gcggatccaa tcccgcaggg agtggtagat
     1141 gacgaccaat aaactcaagc cgaagactgt ccagaccaaa atgggtatgg cgggtccggc
     1201 cataaactgc ggaaagagca gttttttcgt gccgatgtaa aaattaccgc tgaccagttt
     1261 ggaaattgca aaggggtaga gactggtgaa gaccaaaaag tactccgccg cgcgccttat
     1321 gttcgggatc cgcaccggca ttttctggtt gtagagttcg acgacgtagg cgatctggtg
     1381 caggacgtgc atcgaggccc agaagaaaaa gatggtcagg agcaagacct gccgggtgat
     1441 gagcaggtag acgacggcga cggggatgac ggcgaagacg atgaagatgt agtttgagaa
     1501 tctttgggtg aaactcttat ccagcaaaat gcgggtgaag gtggcgtaga tgtgcggccc
     1561 ccccaccaaa agcgccacgg tcagccccac catccagggt ttgaaaccca tccaggagta
     1621 gagggcgtgg gggaagatcg agaggacacc ggaaaaggtg aggagcgaca ggtcccaggc
     1681 ccggctcttc agccacaggt tattgtttcg gtacgagtcg attccgacca aagtaatcct
     1741 ccgtgacagc gcgaagagaa atcatcagca attgcactac cctacggttg actgacccaa
     1801 gtccccgaaa aaagtaagcc ggtatatacg gcggaatgca aagcgttgtc aaggaaaaat
     1861 tggtatggga aagttcccat actgttggcg acccgtacgg gtcgccctac gcaagaagtc
     1921 gggaataata aagccgcgta ggtgcggcgc cctcgccgca cttgaattat ggagatgacg
     1981 gcgctatcat cttgagcaaa gaaattcaca gtcccttttc cgggcgggta ttcgggtcaa
     2041 aaacctcgtt ggtagatcac aagatagcgg gaggaggcga cgggtagttt ggcacacccg
     2101 ttgcagaaag ggtgggtgtg cagaaaccac cgactccatt ctggagagtc accacgtgag
     2161 aaggcaccgc agccctgcgc tcttattctg gattgccatc ggcctgacgc ttctggcgat
     2221 tcttttgacc tacagcgaca tggacggtca aaccgccacc agcacggcca agagcctcat
     2281 ctctctcttc taagtcctgt cacccatacg ggtcgggata ggtttgaggg gggtacctca
     2341 acaaggcgac caggcggtcg cgctcgatta cgagcggggc cgctttcctc ttttatgaat
     2401 agttaaagcc gacctcaagc atttcgccct gttttcccgc ctttagtaat ccttgcggta
     2461 ggacaacacc cattcatata gcattttggg cagtttggct tatttggttt acaccattct
     2521 ctgccaattt cccaggccgg aaggtcgaag actccggggt aggtcgggtt cagttcccgg
     2581 gcgcggaaga ttagttcttc gtttgaagcg ccctccacta tcagaccgag tctagtgaat
     2641 actctccgaa catggacgtc aggagaaatg tcaatggagt tcttgttgcc aataggaatc
     2701 ttgaagtcgc gcacgagaat gtttgccgcc atcggtccaa ttttggggcc cacgccgtca
     2761 aactcgcgaa atcggttaac aatggtgaca ctatcaggac agccagccca tatattagat
     2821 gccttacctg aatacactct cgcaatcttt tgcaccgcag aaaaaaaatt cttgctcatg
     2881 acgatcggga atctgtggag aggttttgga tttgacatta ttctctgtac tctttcgaga
     2941 gataatgctt gaagattggt gaactcaaaa gaaccgagtc gttctttgaa ctcattggga
     3001 atcatccacg ctttttcagc tataatctga cgatccataa tacaggcaag tacgaattca
     3061 tgaggatagc gatctagatc attgagaagt gcatccgctt cttgatgtct tgcaaagact
     3121 attggtctaa aaggttgcga gaggatctct tcgcctcttt tgatcaagat gtctctgata
     3181 gacggctgtg ccattttttc tccaagttat tgtgaggccc cgttacggct ttaggtagaa
     3241 gtgttttttt gtcatagccc gacaatgcaa atatgcatct tttttgtcta agggcaaaat
     3301 ggatgattag catatcttcc tcttaactca agttttgtca cattagaaga ttactccttt
     3361 tgagtcgcgt tcattttgtt aggacaatat gtagagagtc tagttgcccc cttcttaatt
     3421 tccacatccg ggtgtttggc gagcgtggcg aaatcgaagt cgatctcttc cggcttgagc
     3481 gccagccggt agtggccgcg gcggtcgttc tcgatgtcaa tcatatttgg tctttcacga
     3541 ccggtggcat gtaggggtga acctttaggt tcacccgcat tcttgggcga atctaaagat
     3601 tcgcccctac attttctcac acctaactgc tcagcgattt cccgcttcaa c
//
LOCUS       DBNGZD010000396         4395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_618692, whole genome shotgun sequence.
ACCESSION   DBNGZD010000396 DBNGZD010000000
VERSION     DBNGZD010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_618692"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="AC16GM_10300"
     CDS             complement(<1..834)
                     /locus_tag="AC16GM_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924098.1"
                     /translation="MSTKLLALLVATSLFGSPVAGQQLRIDSTANLLPPVVRFLNPTE
                     HTLIVQWRADTIGIGQVYFGTTPKLGLTFAGGTYDTVFSVKLAGLLTATRYHFRLDPP
                     PKNPGGSRFTNDFVYATLAPKGWTEFLRLPVLIVVYTPIHYRDSDSLSSRNESKPARL
                     SDQDVAALKAQGETFRTFFWRNTKCKLDLVLEYLFIDTARADLEKDLEKYVLTIGKKP
                     EDFAGVIFVDTWNDYLDPAKSAKLYRGQALAGETYGVNGPWKFKDLPYSVISPSHGQN
                     VT"
     gene            complement(843..1424)
                     /locus_tag="AC16GM_10305"
     CDS             complement(843..1424)
                     /locus_tag="AC16GM_10305"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2924099.1"
                     /translation="MRFTSGAVGGVSILKIRLLILLLVGAVLPGCGQKKASDERLTTA
                     PYREYLSLAPIVEIHGETADSTQLGDDRYLEDNLDYLLAKHEWVLVNFSAYWCKDCRK
                     FDPDYQAVAGLPKYKGIFFTKAEIDGTKGNENFRTRFALPGIPVTILFHNGQIVEEGG
                     QKAILFGQRGDKTKADLTALLDHFYQPAAKGPA"
     gene            complement(1400..>4395)
                     /locus_tag="AC16GM_10310"
     CDS             complement(1400..>4395)
                     /locus_tag="AC16GM_10310"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR2924100.1"
                     /translation="YTTNTVKDYFMRLDQYLMGLRGSGTVDSLWFIRNPSNGSFACTR
                     AADFTTTVSGQKVWVDINDIIGAEGPRNPDVSTSQKNFRMAFILVVQNGIPPTPYELA
                     RLDSARYQWMSYFRTGVENLGTMNTDLPAPGPLEIVSDTLTKGAVGGTYRDQVFAVGG
                     IPDYPFYALMSGSLPPGLALNTSTGVISGTPTTAGGYNFTVQVCDSSSTCVSKQLHID
                     IYATGTGTVVINEVELFSRGGSVELFNKGSQAKNIGGWKVVTKTLSASDSMIVPANTI
                     IPPGCYYVLTEYAGTNTANWFYFNVTVPWTNGSSGSCALKDNLGTGVDFCRWGSSAEP
                     VPAGTTWSGGNPAAPSGSRNLGRDGNSTDTNNSSDFTSQVGTLGRQNLPYTTQRQTAT
                     TSTPTIQALLTNWNNVASQGSFPTLSYISDGLSFLYDASFFAGLVKLSGDTAVYRSIF
                     SDLLFSLAGPLTVDAVSDPRATHARSASPTIDGLISVDAHYILPKTADSGQFLICQYT
                     VHNLSTDTLSNVLLGMAADYDVTASTSANTSGFDSTKNLVWLQSTDTPSRFAGMAGMA
                     VNRVYAAHALDNPTYVYPANGYVTSQLYQVANTPGFSTAASQPNDLNVVLTQQKTTLP
                     PGECATASFALILSRTNQADLLASADKAKAFLGNLRARYVATTGNDVTGTGTAGNPFA
                     TIQKGIDQAQDGDTVIVQPGTYAGGINVNKPVHLKSALRDSATISGGSPYAVTLSSGC
                     VLDGFIVRSEAVGNILVQSAAGIRPKIVRNRIYSAPVGIQVSNGSNSAIIDHNLISRT
                     VTAISLANTTGSSKITHNTIVANDNGIVVNLSPNGDIVNNIVDSCGNGLVVTGGCPAH
                     SYNDFSNISGSTYAGCVAGVGEIACDPGFCDRSIEDYTLFDNSCAIGAGPGGDTLGAF
                     PVACVACAIATFGDVTGDATADILDIVAVIGYVVFGSPALVPGAFAGDVNCDGVVDIT
                     DIVALINNVVFSAALCNPCDSPLAP"
BASE COUNT          918 a         1230 c         1333 g          914 t
ORIGIN      
        1 ggtgacgttc tggccgtgag agggcgaaat gacgctgtac ggcaggtctt tgaatttcca
       61 cggcccattg acgccgtacg tctcccccgc cagcgcctgg ccccggtaaa gtttggcgct
      121 ctttgccgga tcaaggtagt cgttccaggt gtcaacgaaa attacgcccg cgaagtcttc
      181 cggcttcttc ccgatagtca gcacgtattt ctccaggtct ttctcgagat cggcgcgtgc
      241 cgtgtcgatg aacaggtact ccagcaccag gtcgagtttg catttcgtgt tgcgccagaa
      301 gaaggttcgg aaggtctctc cctgcgcttt gagggcggca acgtcctgat ccgaaagccg
      361 cgccggtttg ctctcattcc gggaagaaag tgagtcggaa tcccgatagt ggatcggtgt
      421 gtaaacgaca attaaaaccg gtaagcgcaa gaactctgtc caccctttag gagccagagt
      481 agcgtaaacg aaatcattgg tgaatcggga acccccggga tttttgggcg gtgggtccag
      541 gcggaagtgg tagcgcgttg ccgtcaaaag tccggccagt ttgacggaaa agacggtatc
      601 gtaggtgccc ccggcaaagg tcaagcccag tttcggcgtg gtgccgaaat agacctgacc
      661 aatcccaatc gtgtccgcac gccactggac tatcagcgta tgttccgtgg gattgagaaa
      721 gcggacgacg ggcggcagaa ggttagcggt ggaatcaata cgtaattgct gtcccgcaac
      781 gggggagcca aagagagagg tggcgacgag caaagccaaa agtttcgtgg acatacggac
      841 gcttacgccg gacctttggc ggcgggctgg tagaagtggt ccaaaagcgc cgtcaaatcg
      901 gctttggtct tgtccccccg ctggccgaaa agaattgctt tctgtccgcc ctcttctacg
      961 atctgaccgt tgtggaagag aatcgttacc ggtattccgg gcagagcaaa gcgcgtccgg
     1021 aagttttcgt tccctttcgt cccgtctatt tcggcttttg tgaagaaaat ccccttgtac
     1081 ttcgggagcc cggctaccgc ctggtaatcg ggatcaaact tgcggcagtc cttgcaccag
     1141 taggcggaaa aattcaccag cacccactcg tgcttggcca aaaggtagtc gagattgtcc
     1201 tccaggtagc ggtcatcccc cagttgggtg gagtccgccg tctcgccgtg gatttcgaca
     1261 atcggggcca gcgacaggta ctcccgatag ggcgcggtag tcagccgctc gtcggaggcc
     1321 ttcttctggc cacagccggg gagaactgcc ccgaccaaca gaagaatgag aagacgaatc
     1381 ttcaggatgg atactcctcc tacggcgcca gaggtgaatc gcatgggttg cacaatgcgg
     1441 cgctgaaaac cacgttgttg atcaaggcga cgatgtccgt gatgtccacc acgccgtcgc
     1501 agttaacgtc accggcaaag gctccgggga ccaaagccgg acttccgaac accacgtagc
     1561 cgatgaccgc tacgatgtcg agaatatctg cggttgcatc acccgtaacg tcgccgaacg
     1621 tggcaatcgc acaggctacg caggccaccg gaaaggcccc taacgtatcc ccgcccggcc
     1681 ccgcgccgat ggcacaagag ttgtcgaaaa gggtgtaatc ttcaatgcta cggtcgcaga
     1741 agcccggatc gcaggcgatc tcaccgacgc ctgcaacaca accggcgtat gtgctccccg
     1801 aaatgttcga gaagtcgttg tacgagtggg ccgggcagcc cccggtaact accagtccgt
     1861 ttccgcacga gtcaacgatg ttgttgacga tgtccccatt cggtgacagg ttcacgacaa
     1921 tcccgttgtc attggccacg atggtattgt gcgttatctt agaagaacca gtcgtgttgg
     1981 ccagagagat ggcggtgacg gttctgctga tcagattgtg gtctatgatt gcactattgc
     2041 tcccgttgga tacctgaatc cccaccggcg cactgtaaat cctgttccgc acaattttcg
     2101 gtcgaatacc ggcggcactt tgcaccagga tattccccac ggcttcactg cggactatga
     2161 atccatccag cacacacccg ctggagagcg tcaccgcgta gggcgacccg ccgctgatag
     2221 tggccgaatc acgcagcgca ctcttcaggt gcaccggctt gttcacgttg attccgccgg
     2281 cataggtgcc cggctggaca atgaccgtat cgccgtcctg ggcctggtca attccctttt
     2341 ggatcgtcgc aaaaggattt cccgcggtgc ccgtaccggt gacgtcatta ccggtggtgg
     2401 ccacgtagcg ggcccgcagg ttccctaaga atgccttggc tttatcggct gaagccaaaa
     2461 gatcggcctg gttggtgcga gagagaatca aggcaaaact ggccgtggcg cattcgccgg
     2521 gcgggagggt ggttttctgc tgggtgagaa cgacgttcaa atcattcggc tggctggcgg
     2581 cggttgaaaa gccgggggtg ttcgccacct ggtagagttg gctggtgacg tacccgttag
     2641 cgggataaac ataggttggg ttgtcgagtg cgtgcgcagc gtaaacacga ttgactgcca
     2701 ttcccgccat cccggcaaaa cgggaaggcg tgtcggtgga ctgaagccag actaagtttt
     2761 tggtggaatc aaacccgctg gtattggccg acgtgctcgc ggtgacatcg taatcggcag
     2821 ccatccccaa gagcacgttc gagagcgtat cggtggagag gttgtgcacc gtgtactggc
     2881 aaatgaggaa ctgcccgctg tcggccgttt tgggcaggat gtagtgggca tcgacgctta
     2941 tgagtccgtc gatggtgggc gaagctgacc gcgcgtgggt cgcccgcggg tcggaaaccg
     3001 catccacagt gagcggaccg gccagggaga acaagaggtc ggagaaaatg ctgcggtaga
     3061 cggcggtgtc gccggagagt ttcaccagcc cggcgaaaaa cgaggcgtcg tagagaaagg
     3121 acagaccgtc gctgatgtag cttaaggtgg gaaagctccc ctgcgaggcg acgttgttcc
     3181 agttggtcaa aagcgcctga atcgtcggcg tgctggtcgt tgccgtctgg cgctgggtgg
     3241 tgtagggcag attctggcgc cctaacgtgc cgacctgcga ggtgaagtcg gaactgttgt
     3301 tcgtgtcggt ggagttgcca tcccggccca agttccggct accggaaggc gccgccggat
     3361 tacctccgct ccaagtcgtt cccgcaggca ccggctcagc cgaactcccc cagcggcaga
     3421 agtcaacgcc agtgcccagg ttgtccttca gggcgcacga accggatgac ccgttcgtcc
     3481 agggaacggt cacgttgaag tagaaccagt tggccgtgtt ggttccggcg tattcggtca
     3541 aaacgtagta gcacccgggc ggaatgatgg tgttggcggg tacaatcatc gagtctgaag
     3601 cagaaagtgt tttcgttacc actttccacc cgccaatgtt cttagcctgg gaacctttgt
     3661 tgaagagttc aaccgagccc ccgcgcgaaa agagttccac ttcattgatt accaccgttc
     3721 cggtgccggt cgcatagata tcgatgtgga gttgcttgga aacgcaggtg ctgctcgaat
     3781 cgcacacctg cacggtgaaa ttgtaccccc cggccgtggt cggcgtgccg gaaatgacgc
     3841 cggtcgaagt attcaacgcc aaccccggcg gcaaactgcc gctcataaga gcgtagaaag
     3901 ggtagtccgg gattccgccc accgcgaaga cctgatcccg ataggtgccg cccaccgccc
     3961 ctttggtcag ggtgtcggaa acaatttcca atggcccggg cgccggcaaa tcggtgttca
     4021 tcgtccctaa gttttccacg ccggtgcgga agtaactcat ccactgatac cgcgcgctgt
     4081 ccagccgggc cagttcgtat ggcgtcggcg ggatgccgtt ctgaaccact aagataaaag
     4141 ccatgcggaa gtttttttgc gaggtggaga catccggatt ccggggccct tccgccccga
     4201 tgatgtcatt gatgtccacc cacaccttct gcccgctcac cgtcgtggta aaatcggccg
     4261 cgcgggtgca ggcgaaactc ccgttcgagg gattccggat gaaccagagc gaatcgaccg
     4321 tgcccgagcc ccgcagcccc atcaggtact ggtctaaccg catgaaatag tctttcaccg
     4381 tgttggtggt gtaag
//
LOCUS       DBNGZD010000397         1147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1381945, whole genome shotgun sequence.
ACCESSION   DBNGZD010000397 DBNGZD010000000
VERSION     DBNGZD010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1381945"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="AC16GM_10315"
     CDS             complement(<1..>1147)
                     /locus_tag="AC16GM_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013524.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HIR2924101.1"
                     /translation="GRTPASDGRDSESKVYAADEPFPGKYAELKSQGIFDGNHIATLA
                     LYPYQYLPFEKRVSFTSELQVTLITRPGSLTEVQRISTNSPAQWFVTQPTAGAKTPVS
                     GGTALSGVQGQSSGCPLGAAYLLVTGARLADAFAPLVEWKKKKGLSAATALIDSVVKH
                     YPGRDDAERLRNYLIACYQSGTVWVLLGGDETVVPIRYAFYTDAAAAPPSTDLAICDL
                     YFADVNGNWDKDGDGIWGEPTQDQPDLYPDLFVGRVPAADSIAAANFVEKLLTYERNP
                     GYGDPAYLTRVMHMSSDQMRDWPVGVGQHGLVGAKFPPLFQQDLTTLVEAPAGNAPNP
                     ASPDGPFSVQRLSQGQGFVTIFAHGRPDGFFARSHLVAQSPISFVFTW"
BASE COUNT          238 a          322 c          374 g          213 t
ORIGIN      
        1 cccaggtgaa aacgaacgat atcggcgact gagccaccag gtgggagcgg gcaaagaagc
       61 cgtcgggccg gccgtgggcg aaaatggtca caaatccctg cccctgcgaa agtctctgga
      121 ctgagaacgg accatccgga ctggccggat tcggcgcatt gccggcggga gcctcaacga
      181 gtgtcgtcag gtcctgctga aagagagggg ggaatttggc gccgaccaag ccgtgctggc
      241 cgaccccgac cggccagtcg cgcatctggt ccgaactcat gtgcatcacc cgggtgaggt
      301 aggcagggtc accgtacccc ggatttctct cgtaggtcaa gagtttttcg acgaagttcg
      361 cggctgcgat ggaatccgcc gcgggaacac gcccgacgaa cagatccgga tagaggtcgg
      421 gctggtcctg ggtcggttcg ccccagatgc cgtcgccgtc cttatcccag ttgccgttca
      481 cgtcggcgaa gtagaggtcg cagatcgcca agtcggtcga cggtggtgcg gcggcagcgt
      541 cggtgtaaaa ggcatagcgg atcgggacaa ccgtttcgtc gccgcccagg agtacccaaa
      601 cagttccgga ctggtagcag gcgattaggt agttgcgcag acgttccgcg tcgtcgcgcc
      661 cgggatagtg ctttaccacc gaatcgatca gtgcggtggc ggcggaaaga ccctttttct
      721 ttttccactc caccagagga gcaaaggcat cggccaaacg agcgccggta accagaagat
      781 aggccgcgcc caggggacag ccggaagatt gaccctggac acctgacaaa gcagttccgc
      841 ccgacacggg agtttttgct ccagccgtcg gttgcgtgac gaaccattgc gcgggagagt
      901 tggtggaaat tctttgaacc tccgtcagcg aaccgggccg ggtgatcagc gtaacctgca
      961 gttcggaggt aaaggagact cgcttttcaa aagggagata ctgatacggg tataaggcca
     1021 gcgtggcgat atggttcccg tcaaaaatgc cctggctctt cagttcggcg tacttgccgg
     1081 ggaagggctc gtcggctgcg taaacttttg attcagagtc cctgccatcc gatgcggggg
     1141 ttcgacc
//
LOCUS       DBNGZD010000398         4139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_549843, whole genome shotgun sequence.
ACCESSION   DBNGZD010000398 DBNGZD010000000
VERSION     DBNGZD010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_549843"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="AC16GM_10320"
     CDS             <1..424
                     /locus_tag="AC16GM_10320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR2924102.1"
                     /translation="PYNLKEPKRGILLIALAGPVANMIIALACGLIIRFSSGAYDPHN
                     TTITGIIFKMVVQSLLLNLSLAFFNLIPVPPLDGSKIMYGLLPDRYAYQFAMWERVGP
                     LVLIGLILLGSFSGFNILWFILSPFVRLFAFLFAGISF"
     gene            434..1231
                     /locus_tag="AC16GM_10325"
     CDS             434..1231
                     /locus_tag="AC16GM_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924103.1"
                     /translation="MSGHLKLLWTATGVVLLMVFTGCAGPRSISRVDPVAVLSQAKQE
                     RTSTAFTTASFRVKFSLSTQAGSGAGIAYLYAKKPDTLAIEVPGVFGAGAFRARIDGA
                     SHLFIYIPEEKAYYDGLLAQFPAEGIFWTPTVWQSPAWDVFFGNSSPLGGDDDTLTFV
                     GERKGLYFYGEDDQGWQHEIGINPRDGKARQELWITEDSAVIWRIRYGRSRQGKTGPP
                     IDRIDLQTEWGEKFSLQFQEANFDVPISPRKFSTDVPAGARRIEPLR"
     gene            1238..2362
                     /locus_tag="AC16GM_10330"
     CDS             1238..2362
                     /locus_tag="AC16GM_10330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR2924104.1"
                     /translation="MKRFEKLIKQAALRLLRLIWGNSPIPGAFRREAVRSIVVVRQDE
                     RLGNMVLITPFLSGLRKAFPDASLTLVASEKYGHLFDDDNCIDRLVLVNKKRCLFNPV
                     YLLKTIQQLRNIRADLAFDLSDAEQISALNSIATRLTGARWRIGFDRPEAATFLNRLV
                     PVPEVPCHATEMHLRLLQALVPGSYDSAPAIRVDAGAREWAKEWLQRKRQPLESRLVA
                     VHIGARGPKQWGVENFFELIESLTADGNVKVVVITGPQEHHLLEQKKKDDRNVLLAPK
                     MSVPQLAALLSLCDLYVGNDAGPLHLAVAVGTPTVALFFTSDALKYGPRGKKHIILAT
                     LGRRPSVAEVYRSIHLALTGIDRPGGRSRSTQVAVAAGIP"
     gene            2371..>4139
                     /locus_tag="AC16GM_10335"
     CDS             2371..>4139
                     /locus_tag="AC16GM_10335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2924105.1"
                     /translation="MQPKRSHYRRLVRFLKPYWKHATVSMVSMTLFALLSGAMIWMIG
                     PLAGTLFGLGGKSVMTAQTPDSANQTGTPELLKPYSGLKAKLKAPFERFFHHPDKLTV
                     LERLCIAVLLIVVLKSLFFYLQGYLMAYVQQSVIRDLRNTLYRHYHTLSLSFFNKART
                     GQLISRITNDVAVVNEMLEMALTRLVRDPILVIIFIFILLVISWQLTLVAFVAFPLLM
                     LGVSQIGKAIHRYSQRSQERIADVNAAIQEAVSGVRVVKAFGAERYEVAKFEALTKAY
                     RKVMLKMGRVRILSSPINEILATAAGVVILWFGGKQVLSGHLLKPEDFLLYLFAMFSL
                     IAPAKSLSLAHAKVQEGMAAAARIFEIIDTAPHIVEDPQAQPVTGFRLKIVFADVSFR
                     YDTGPWVLRDINLSVNAGEVVAVVGPSGSGKSTLVDLLSRFYDPTEGSIALDGLDLRR
                     IKTGSLRTLIGIVTQETILFHDTVFNNIAYGLEGVTELRVKGAAEAANAADFIEKLDH
                     GYQTIIGDRGVRLSGGQRQRLAIARALLKNPPILIFDEATSALDSESERLVQEAIARL
                     VTGRTTFLIAHRLSSVLRADRIIV"
BASE COUNT          844 a         1182 c         1166 g          947 t
ORIGIN      
        1 tccatacaat ctgaaagagc cgaagcgcgg gattctcttg attgctttgg ccgggccggt
       61 ggcgaatatg attattgccc tggcctgcgg gctgatcatt cgtttttcct ctggagcgta
      121 cgacccgcac aatactacga tcacaggcat aatctttaaa atggtggtgc agagtctcct
      181 gctcaatctg tccctcgctt ttttcaacct gattccggtg ccgcccctcg atggctcaaa
      241 aattatgtac gggctattgc ccgaccgcta tgcctaccag ttcgccatgt gggaacgggt
      301 ggggcctctg gtgctcatcg ggcttatcct tctcggaagc ttcagcgggt tcaatattct
      361 gtggttcatc ttatctccct tcgtccgcct gttcgccttc ctttttgccg ggatttcatt
      421 ttaaagacgc aggatgtccg gccatctcaa actgctctgg acggcaacag gtgtcgtctt
      481 attgatggtt ttcaccggct gcgccgggcc gcgctccatc agccgcgttg acccggttgc
      541 cgtcctatcg caggcgaagc aggaacgaac ctccactgct ttcaccaccg ccagtttccg
      601 ggtgaaattt tcgctttcaa cccaagccgg ttcgggtgcg ggaatcgcct acctgtatgc
      661 caaaaagccc gatacgctcg ccatcgaagt gccgggcgtt ttcggcgcgg gagcattccg
      721 cgcgcggatt gacggggcgt ctcatctttt catttacatc ccggaagaaa aagcgtacta
      781 cgacggcctg ctggcgcaat tccccgccga gggaattttc tggacgccga cggtctggca
      841 gagcccggcc tgggacgtgt tcttcggcaa ttcgtccccc ttgggcggag atgatgacac
      901 gttgacattt gtcggggaga gaaaagggct gtacttttat ggagaggatg atcaaggatg
      961 gcaacatgaa atcgggatca atccccgcga cgggaaggcc cgtcaggaac tctggatcac
     1021 ggaggacagc gcggtgatct ggcgcatccg ctacggacgc agccgtcaag gaaaaacagg
     1081 accgcccatt gaccggatcg atttacaaac agagtggggt gaaaagtttt ccctccaatt
     1141 tcaggaagcc aatttcgacg tgccgatttc gccgaggaaa ttcagtaccg acgttcccgc
     1201 gggggccagg cgaattgaac cgcttcgctg aacaggtatg aaaagattcg aaaaactcat
     1261 taagcaggcg gcactaagac ttttgcgcct tatctgggga aattcaccca ttcccggagc
     1321 gttccgcagg gaagcggtgc gttcaattgt agtggtgcgg caggatgaac gcctggggaa
     1381 tatggtgctc attacgcctt tcctttcggg tttgcgtaag gcgtttcccg acgcttctct
     1441 taccctggtg gccagtgaga agtacggtca tctttttgac gacgataatt gcattgatcg
     1501 gctcgttctg gtgaataaga aacgctgtct attcaatccg gtctatctcc tgaaaactat
     1561 tcaacagctt cgcaatatcc gagctgacct cgcttttgat ttaagcgacg ccgagcaaat
     1621 ctcagcgctc aatagcatag cgacccgcct taccggcgcc cgctggcgga tcggctttga
     1681 ccggccagag gccgccacat tcctcaatcg gttggtaccg gtgcccgaag ttccctgtca
     1741 cgcaaccgag atgcacctgc ggctgttgca agcactggtt cccggctcgt atgattccgc
     1801 gccggcaatt cgggtcgatg ccggtgctcg tgagtgggca aaagagtggc tgcaaaggaa
     1861 gcgacagccg ctggagtcgc gtttagtcgc tgttcatatc ggcgcccgcg gcccgaaaca
     1921 gtggggtgtg gagaatttct ttgaactcat agaaagcttg accgccgacg gcaacgtgaa
     1981 agtggtcgtt atcaccgggc cacaagagca tcatcttctg gagcagaaaa agaaagatga
     2041 tcgaaacgtc ctgcttgctc ccaaaatgtc agtaccccaa ctggcggctt tgctgtcgct
     2101 ctgcgacctg tacgtaggca acgacgccgg tcccctccat ctggctgtgg cggtgggaac
     2161 gccgacggtt gcgttatttt tcacttcgga tgcactgaag tacgggccgc gggggaaaaa
     2221 acatattatt ctcgccactc tagggcggag gccgtcggtt gccgaagtct atcgatcaat
     2281 tcatttagcg ctgacaggaa tcgatcgtcc gggtggaaga agccgatcga ctcaagtagc
     2341 cgtcgccgca gggataccgt gagcactccc atgcagccga aacgctccca ctaccggcga
     2401 ctggttcgct tcctgaaacc gtactggaag catgccacgg tgtcgatggt gagcatgacc
     2461 ctctttgcgc ttttgagcgg ggcgatgatc tggatgatcg gccctctggc gggaacgctc
     2521 ttcggtctgg ggggaaaaag cgtcatgacc gcccagaccc cggacagtgc gaaccagact
     2581 ggcacgcccg aactcctgaa gccctattcc ggtctcaagg ccaaactcaa agcgccattc
     2641 gagcggtttt tccatcaccc ggacaaactg acggtccttg agcgcctctg catcgccgtt
     2701 ttgctcatcg tggtgttgaa aagcctcttc ttctacctgc agggctacct gatggcctat
     2761 gtacagcaga gcgtcatccg cgacctgcgc aatacgctct accggcacta ccatacgctc
     2821 tcgctttcct tcttcaacaa ggcccgcacc ggccagttga tctcccgtat caccaacgac
     2881 gtggcagtgg tgaacgagat gctcgagatg gcgctcacgc gcctggtccg cgatccgatc
     2941 ttagtcatta tttttatctt catcctcctg gtgatttcct ggcagttgac gctcgtggcg
     3001 ttcgttgcct ttcccctctt gatgctgggg gtctcccaga tcggcaaggc gattcaccgc
     3061 tacagccagc gctcgcagga gcggatcgcc gacgtgaacg cggcgatcca ggaagcggtc
     3121 tccggtgtcc gggtggtgaa ggccttcggc gctgaacgct acgaggtggc caagtttgaa
     3181 gcgttgacca aggcctaccg gaaggtgatg ctcaaaatgg gccgggtacg catcctctcc
     3241 agcccgataa atgaaatttt agccaccgcc gcgggagtgg tcattctctg gtttggagga
     3301 aaacaggtgc tctccggcca cttgctcaag ccggaagatt ttcttttgta tctttttgcg
     3361 atgttttccc tcattgcacc ggccaagagt ttgagcctgg cgcatgcgaa ggtacaggag
     3421 gggatggccg ccgcggcgcg catttttgaa attatagaca ctgccccgca tatcgtcgaa
     3481 gacccgcagg cccagcccgt caccgggttc cgactaaaga tcgttttcgc cgacgtctct
     3541 ttccgctacg acacgggacc ctgggtgttg cgcgacataa atctctcagt caacgccggt
     3601 gaggtagtcg ccgtcgtggg gccctccggc tcgggcaaat cgaccctggt agacctcctc
     3661 tcgcgttttt acgaccccac cgaggggagc atcgctctcg atggtctcga tttgcggcgc
     3721 atcaaaactg gaagtctgcg gaccctgatc gggatcgtca cgcaggagac gattctcttc
     3781 cacgacacgg tgttcaacaa catcgcctac ggtctggagg gggtcaccga acttcgggtg
     3841 aaaggcgcgg cggaagcggc gaatgcggcc gacttcatcg aaaaattaga tcacggctat
     3901 caaacgatta tcggcgaccg gggcgtgcgc ctctcggggg gacaacggca acggctggca
     3961 atcgcccggg cgcttctgaa gaacccgccg attttgattt tcgatgaagc aacctctgcg
     4021 ctcgactcag aatcagaaag gttagtgcag gaagcgattg cccgcctggt caccggtcga
     4081 accacatttt tgattgctca ccggctgtcg tcggttctgc gcgccgaccg catcattgt
//
LOCUS       DBNGZD010000399         2236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1099418, whole genome shotgun sequence.
ACCESSION   DBNGZD010000399 DBNGZD010000000
VERSION     DBNGZD010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1099418"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1513)
                     /gene="trpE"
                     /locus_tag="AC16GM_10340"
     CDS             complement(38..1513)
                     /gene="trpE"
                     /locus_tag="AC16GM_10340"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR2924106.1"
                     /translation="MDSFVEFKKKAAQGNVVPLVAEMPADLMTPVGAFLRVARRARYS
                     FLLESVEGGEKLGRYSFIGLDPYRLFACTQQGIEIREQRKRLALSTDPFAYLRESFNG
                     CQPAFDPRLPRFSGGAVGYFGYDAVRLFERVPDKNPDPLKLPEIFFGFYDLVLAFDHL
                     RQKVFVICNLFTDRKGEPLPRQYREGVRRVEAVLSLLSGPLPARRSATRPPRRAQVTS
                     NLSQEQFEAKAQAAKDLIAAGDIFQVVLSQRFQRGCAALPFAVYRALRMVNPSPYMFF
                     LKMDDVTLVGSSPEMLVRVDDGSVETRPIAGTRPRGQSEADDRRLAAELLVDPKERAE
                     HVMLVDLGRNDIGRVSQPGKVRVEDLMHIERYSHVMHIVTSVKGTLRPEVDRLAALAA
                     CFPAGTVTGAPKIRAMQIIDEFENVRRGVYAGAVGYLDFQGDLDSCIAIRTIVIRNRT
                     AYVQAGAGIVADSIPEREYQETLDKAQALFSAIELAERGMV"
     gene            complement(1516..>2236)
                     /gene="dapF"
                     /locus_tag="AC16GM_10345"
     CDS             complement(1516..>2236)
                     /gene="dapF"
                     /locus_tag="AC16GM_10345"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR2924107.1"
                     /translation="VGGDGLVLLRRGRRYPFRMEIYNADGSQAEMCGNGIRCLARLIY
                     EAGHSTSRTQAIETRGGIIRTEITSLSNGQRPFTVRVDMGRPELAVKRIPVRSEQKHF
                     VNQSVRIGLQKINLTCLSIGNPHAVTFVRALPENWALLAQEIAALRLFPKGVNVSFAS
                     VQGRRKMTLRVWERGAGATQACGTGACAAAVAAVLNGLAERTVKVHLPGGSLKVEWDQ
                     KSDHVFLTGPAVRVFSGRFEF"
BASE COUNT          458 a          725 c          623 g          430 t
ORIGIN      
        1 ttgtaggtga acgagtcgta gttgtctatc agaacgatca caccattccc cgttctgcta
       61 actcgattgc cgaaaacaac gcttgcgctt tgtcgagcgt ctcttggtac tcccgctccg
      121 gtatcgaatc ggctactatt cctgcacccg cctgcacgta ggcggtccgg ttccggatga
      181 cgatggtgcg gatggcgata caggagtcga ggtccccctg gaaatcgagg taacccaccg
      241 ccccggcgta gaccccgcgg cgcacattct cgaactcgtc gatgatctgc attgcgcgga
      301 ttttcggcgc accggtcacc gttcccgccg gaaagcaggc cgccagcgcc gccagacggt
      361 ctacttcggg tcgcaacgtc cccttcaccg aagtgacaat gtgcatgacg tgggagtaac
      421 gctcgatgtg cattaaatct tcgactctga ccttcccggg ctggctgacc cttcctatat
      481 cgttgcggcc caaatcgact aacatcacgt gctcagcccg ctccttgggg tcaaccaaaa
      541 gttccgccgc caggcggcgg tcatcggctt cgctctggcc gcggggacgc gtgccagcaa
      601 tcggccgggt ctccaccgag ccgtcatcaa cgcgcaccag catctccggc gaggagccga
      661 ccaaagtcac gtcatccatc ttcaggaaaa acatgtacgg cgacggattg accatccgca
      721 gcgcccggta gaccgcaaac gggagggccg cgcacccccg ctggaagcgc tgcgacagca
      781 ccacctggaa aatgtcgccg gcggcgataa ggtctttggc cgcttgcgct ttcgcttcaa
      841 attgttcctg agaaagattg gaggtgacct gtgctcggcg gggaggccgt gtcgcggacc
      901 gccgcgcggg caagggcccg gagagcaggg agagaaccgc ctccacccgc cgcaccccct
      961 cccggtactg ccgcgggagc ggttctccct tccggtcggt gaagagattg cagatcacaa
     1021 aaactttctg ccgcaggtgg tcgaaggcca ggaccaagtc gtagaatcca aagaaaatct
     1081 ccggcagttt cagtggatcg ggatttttat ccggtacccg ttcgaacagc cggactgcat
     1141 cgtacccgaa gtaccccacc gcaccgcccg aaaagcgcgg caggcgcggg tcaaacgccg
     1201 gctgacaacc gttgaaggat tcccgcaagt atgcgaacgg atcggtggac aaggcgagtc
     1261 tttttctctg ctcgcggatt tcaatcccct gctgcgtgca ggcaaacagc cggtacggat
     1321 cgaggccgat gaaggagtaa cgccccaact tctccccgcc ttccacactc tctaagagaa
     1381 acgagtaacg cgcccggcgg gctacgcgca ggaaggcgcc caccggcgtc atgagatcag
     1441 ccggcatttc cgccaccagc ggaaccacgt ttccttgcgc cgctttcttt ttgaattcaa
     1501 cgaacgaatc catacctaaa actcgaacct gccggaaaag acccgcaccg ccggcccggt
     1561 caaaaagacg tgatcgcttt tctggtccca ctccactttc agactgccgc cgggcaggtg
     1621 gactttgacc gtgcgttccg caaggccgtt gagcaccgcc gccacggcgg cggcgcaggc
     1681 cccggtgccg caggcctgcg tggctccggc tccccgctcc cagacgcgca aggtcatttt
     1741 tctccgacct tgcacgctgg cgaagctgac gttgacgcct ttcgggaaaa gccgcagcgc
     1801 ggcaatttct tgagccagaa gagcccaatt ttccgggagt gcccggacga aagtaaccgc
     1861 gtgtggattg ccgatagata aacaggtcaa attgatcttc tgcaaaccaa tgcggaccga
     1921 ttggttaaca aagtgtttct gctcagagcg taccggaatt ctctttacgg ccagttccgg
     1981 cctccccata tccacccgca ccgtaaaagg tctttgcccg ttgcttaggg aagtaatttc
     2041 cgttcggatg attccgccgc gcgtctcaat cgcctgcgta cgacttgttg agtgtccggc
     2101 ttcgtaaatg agccgcgcca gacagcgaat gccgttcccg cacatttccg cctgcgagcc
     2161 atcggcattg taaatctcca tgcggaaggg ataccttctc ccccgccgca agaggacgag
     2221 cccatcccca ccgacg
//
LOCUS       DBNGZD010000400         4177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_479408, whole genome shotgun sequence.
ACCESSION   DBNGZD010000400 DBNGZD010000000
VERSION     DBNGZD010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_479408"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="AC16GM_10350"
     CDS             complement(<1..679)
                     /locus_tag="AC16GM_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HIR2924108.1"
                     /translation="MKTFLGIFEDDTYANFFPVTFNRPVWLLRSGIRLLWEKIEAAFP
                     GTTVGFSCRDLLAETAAEQVTGSVNVLPETAERIVLVNGRLAVDRSGADVIAGTQPPA
                     VFLDGETVAAIILDPKTAGAYLSNLRSLWERGAQRQLVDRLPRKPFKGRWTNYLWELV
                     AANGDEIARDFAALAPALDFKKMFDRCEVDQQTVIHKVEQVYLGEGAKIDAGTVLDAR
                     SGPIFIDR"
     gene            complement(881..1444)
                     /locus_tag="AC16GM_10355"
     CDS             complement(881..1444)
                     /locus_tag="AC16GM_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR2924109.1"
                     /translation="MAAIFFLLVILFATPVVLFIVYYNRLVRLRTGSEAAWSQVDVEL
                     QRRADLVPNLAETVRGYAGHERGVFENVALARSRWQEAKTIPEKAQASEFLAGTLKSL
                     FAVAENYPALLASQNFRELQQQLTEIETRIAAARKAYNEICFQYNTARQTFPSNLVAG
                     MFTFAGLDYFQPEGPAARAVPKVEFGR"
     gene            complement(1445..2242)
                     /locus_tag="AC16GM_10360"
     CDS             complement(1445..2242)
                     /locus_tag="AC16GM_10360"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2924110.1"
                     /translation="MRTQHFKPETGWTFTFSVVAAAVLIFFPRPSGATISEDAKKHFN
                     AGVEAKKTGNSDKAIAEYEQAIAAAPEYVEARVNVANLYETKGLVPKAIEQFKALTQL
                     EPGKAEYRSKLAALYVRNRQFADAGALYEEITAKNPKDGTAWLDLAEVYKKTGQPAKA
                     VEAYKQAIAADPQNVEAHYNLGNAYLGKGLYGEAQKEYREVMKLKPVHYKAAFNLAVA
                     VQSQDPESLQQFLPLYEEFVQKFKGNAQARPMVQKAQEIIKQGKGAK"
     gene            complement(2259..2753)
                     /gene="purE"
                     /locus_tag="AC16GM_10365"
     CDS             complement(2259..2753)
                     /gene="purE"
                     /locus_tag="AC16GM_10365"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR2924111.1"
                     /translation="MATSKLKVPILILLGSTSDRPAMAGCEAVLGELGIGYRLEISSA
                     HRHPDRTRRLARTAERQGYQVIITAAGLAAHISGVVASESLLPVIGVPLAGSPLAGLD
                     ALYSMVQMPRGVPVATMALASSGPVNAAIFAAQILAIEDRQVKTRLTRWKAKLARRSS
                     PKRS"
     gene            complement(2766..4100)
                     /gene="purD"
                     /locus_tag="AC16GM_10370"
     CDS             complement(2766..4100)
                     /gene="purD"
                     /locus_tag="AC16GM_10370"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR2924112.1"
                     /translation="MNVLVVGSGGREHALVWKIAQSPRVKKIYAAPGNAGIAQETLGP
                     HERVTCVPIQPTDFTGLVEFARANQIDLTLVGPEQPLCLGIADEFLLHGLKIVGPTRK
                     AALIEGSKAFAKDFMQRRNIPTASFCVFTQFESARTFVQQADYPLVIKASGLAAGKGA
                     FVVESVEEAAAVLEQLLVQRTLGEAGATVVVEKFLEGVEATVMAFTDGKTVVPLPPVH
                     DYKRAYEGDRGPNTGGMGAVAPTDRLDQKTLNTIVAEILEPAVNGLAAEGRPYQGILY
                     AGVMLTESEPKVLEFNCRFGDPETQAVLPLMKSDLVEILLALADRELELVPIDWSGQY
                     ACSVVLAAGGYPEKYETGKEIFGLRPREQEGTLIFSAGIKSDRGRLVTDGGRVVSVTA
                     IHDRPDQAVARAYVAAERIKFEGKQFRRDIGFSASRIERAAPRDRGKFAAVR"
BASE COUNT          769 a         1360 c         1142 g          906 t
ORIGIN      
        1 gccggtcgat gaaaatcggg ccggagcgtg cgtcgagcac cgtgccggca tcgattttcg
       61 ctccctcgcc gaggtagacc tgctcgacct tgtggatcac ggtctgctga tcaacttcgc
      121 accggtcgaa catttttttg aaatccaagg ccggcgctaa ggccgcaaag tcgcgggcga
      181 tctcgtctcc gttcgccgcg actaattccc agaggtaatt ggtccagcgc cctttgaaag
      241 gtttccgcgg cagccggtct actaattgcc gctgcgcccc ccgttcccac aaactgcgca
      301 ggttggagag atacgccccc gccgtctttg gatccaggat aatcgccgcc accgtctcgc
      361 cgtccaaaaa gacggccggt ggctgggtgc cggcgatgac gtccgccccg ctgcggtcga
      421 cggcaagccg tccgttgacc agaacgattc gctccgcggt ttccggcagc acgttgacgc
      481 tcccggtcac ctgttcggcg gctgtctcgg ccaaaaggtc ccggcaagag aacccgaccg
      541 tcgtccccgg aaaggccgcc tcgattttct cccacaaaag ccggatgccc gaccgcaaaa
      601 gccacaccgg ccggttgaag gtcaccggga aaaagttggc gtaggtgtcg tcttcgaaga
      661 tgccgaggaa agttttcata ggttatatat cggtctatct gttaactctc ccggcaactg
      721 cagccacgcg ctcttaagcc tgagagaaac tgaaggcctg agcgtggccg gtcgttgccc
      781 ttccactttt gaccccagcc caagaccctg aattgtcgat ttatggcggg aatttgcggg
      841 aataaaccgt cggtttggcg tcccggtgaa acttttagcc tcaccggccg aactccactt
      901 tcggaaccgc gcgcgcagcc ggtccttctg gctggaaata gtcgagtccg gcaaaagtga
      961 acattccggc cacgaggttt gaggggaaag tctgccgggc cgtgttgtac tggaagcaga
     1021 tctcgttgta cgcctttcgc gccgcggcga tgcgggtttc gatttcggtc aactgctgct
     1081 ggagttctcg gaaattctgg ctggccaaaa gcgcgggata attctctgcc acggcaaaaa
     1141 ggctcttcag cgttcccgcc aaaaactccg acgcctgggc cttttccggg atcgtcttgg
     1201 cctcctgcca ccggctgcgg gccaaagcga cgttctcgaa caccccccgc tcgtgtccgg
     1261 cgtaccctcg cacggtttcg gccaagttgg ggacgagatc ggcccgccgc tgcagttcca
     1321 catccacttg cgaccaggcg gcctcgcttc ccgttcgcaa gcggacgagc cggttgtagt
     1381 agacaatgaa cagtaccacc ggcgtggcga agaggatgac cagaaggaaa aatatcgccg
     1441 ccatctactt cgcgcctttg ccctgtttaa taatttcttg ggctttctgc accatcggcc
     1501 gcgcctgcgc gttgcccttg aatttctgga caaactcttc gtagagcggc aaaaactgct
     1561 gcaaactttc gggatcctgg ctctggacgg caacggccag gttgaaggcg gccttatagt
     1621 gcaccggttt caacttcatc acttcccggt attctttctg cgcttcgccg tacaggcctt
     1681 tacccaaata ggcattacct aaattataat gcgcctctac gttctgcgga tcggcggcaa
     1741 tcgcctgctt gtaggcctcc accgccttgg ctggctgccc cgtttttttg tagacctcgg
     1801 ctaaatcgag ccaggcggtc ccgtccttgg gatttttggc agtgatttcc tcatagagcg
     1861 caccggcgtc ggcgaactgc ctgttcctga cgtagagcgc cgccagtttg ctccggtact
     1921 ccgctttgcc cggctccagt tgcgtcaggg ctttgaattg ctcaatggct ttgggaacga
     1981 gacccttggt ctcgtacagg ttggcgacgt tgacccgcgc ctccacgtac tccggtgccg
     2041 cggcaatggc ctgttcgtac tcggcaatgg ccttgtcgct attccccgtc ttcttggcct
     2101 ccacaccggc attgaagtgc tttttggcat cctccgaaat cgtggcacca gagggtcttg
     2161 gaaaaaaaat cagaaccgcc gctgccacaa ccgagaaggt gaaggtccac cctgtttccg
     2221 gtttgaaatg ttgcgtacgc attgccgctc ctttttcttt aagacctttt cggactgctc
     2281 ctccgtgcca acttcgcctt ccagcgcgta agccgggttt ttacctgccg atcctctatt
     2341 gccagaatct gcgctgcaaa aatggcggca ttgaccggcc cgctggacgc caaggccatc
     2401 gtcgccaccg gcacccctcg gggcatctgc accatcgagt agagcgcatc cagtccggcc
     2461 aaaggcgagc ccgcaagcgg cacgccgatc accgggagga gtgattccga cgccaccacg
     2521 ccggagatat gggccgccag cccggcggcg gtgattatca cctggtaccc ctgccgctcg
     2581 gccgtccgcg ccagccgccg agtccggtcg ggatgccggt gggccgaaga aatctccaac
     2641 cggtagccga tccccagttc cccgagcact gcttcacagc ctgccatcgc cgggcgatcc
     2701 gacgtactgc ccaggaggat caaaatcgga actttcagct tactggtggc cataacccgc
     2761 attctttacc ggacggctgc gaacttgcct ctgtctctgg gggccgcccg ctcgatccgg
     2821 ctggcgctga aaccaatatc tctacggaac tgctttcctt caaatttgat ccgctcagcc
     2881 gccacatagg cccgggccac cgcctgatcg gggcggtcgt gaatcgcggt gacactcacc
     2941 acccggccgc cgtcagtgac caaccggccc ctgtcacttt tgattccggc agagaagatg
     3001 agcgttcctt cctgttctct gggccggagc ccgaaaattt cttttcccgt ctcgtatttt
     3061 tccggatacc cccccgcggc cagcacaacg gaacaggcat actgacccga ccagtcgatc
     3121 ggcaccagtt ccagttcccg atcggccaaa gccagcaaaa tctccaccag gtcacttttc
     3181 ataagtggca gcaccgcctg cgtttccgga tccccaaagc ggcagttgaa ttctaacact
     3241 ttcggctcgc tttcggtcag catcaccccg gcgtagagga ttccctgata gggccgtcct
     3301 tcggcggcca gaccattcac cgccggctcc agaatctccg cgacgatcgt gttgagcgtc
     3361 ttctgatcca gccggtcggt cggcgccacc gctcccatcc cccctgtatt gggtccgcgg
     3421 tcgccctcgt aggcccgctt gtagtcgtgg accgggggca gaggcacgac tgtcttccca
     3481 tcggtaaagg ccatgaccgt ggcttccacg ccctccagga acttctccac tacgacggtc
     3541 gctcccgctt cccccagggt tctctgaacg agcagttgct cgagtacggc ggcggcttcc
     3601 tcaacacttt ccaccacaaa agcacccttg ccggccgcca gaccgctggc tttgatcacc
     3661 agggggtagt ctgcctgttg tacgaaagtt cgggcggatt caaactgagt gaatacgcag
     3721 aaactggcgg tcggaatgtt tcggcgctgc atgaaatctt tggcaaaggc tttactcccc
     3781 tcgatcagcg ccgcttttct tgtcggcccg acgatcttca gtccgtggag gagaaactca
     3841 tcggcaatcc ccagacagag cggctgctcc ggcccgacga gggtgaggtc aatctgattg
     3901 gcgcgggcaa actcgactaa gcccgtgaaa tcggtcggct gaatcggcac gcaggtcacc
     3961 cgctcgtgcg gcccgagcgt ttcctgggcg atcccggcgt tccccggcgc cgcatagatt
     4021 tttttgaccc gggggctctg ggcgattttc cagaccagcg cgtgctcccg cccgcccgac
     4081 ccgaccacca ggacgttcat ggtaagaaga atggcctccc cagctctcgc cggctcattt
     4141 caaccttttt cattcgtcgc ttaaattaac cttcgca
//
LOCUS       DBNGZD010000401        10524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_341280, whole genome shotgun sequence.
ACCESSION   DBNGZD010000401 DBNGZD010000000
VERSION     DBNGZD010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_341280"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1364)
                     /gene="tig"
                     /locus_tag="AC16GM_10375"
     CDS             complement(18..1364)
                     /gene="tig"
                     /locus_tag="AC16GM_10375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR2924113.1"
                     /translation="MPEIVSASTIKVKVTDQGSWRRRLEIEVPPERIAAQFAATYEQF
                     KAKAKIPGFRPGKAPLDLVKERFAGLARQEVLEELVPQAYREAVAQTDLHPITEPEVK
                     DVQFSNGQPLRFAAEFEIRPVAELKKYTGFKFSKPKYEITDADVTEFLTRQRERKAQL
                     KPVARAAASGDVVVATIEKLSDRENRIPPFKKENAAIDLEQTRDLDDFAKEIVGMRVG
                     EIKEFDVNYPADFSEQAYAGNRVRYWVLAKEVKEKILPPLDDEFAKQEGFTALADMRE
                     KVQQTLTAEMEREAESRLRAQVRHTVVEQNHFPVPQSLLATYLDGVAADFRKNYQNFD
                     EKQVRESYRPLGEELIQWEILLRQIAEKENLAPTKEDTNAWLSRFAARYQITPEEAQE
                     TLAKSGKIKDVRETILEQKVIDHILKHSEVSETPVAKETASGTKQTGKEKTSKLIT"
     gene            complement(1384..1468)
                     /locus_tag="AC16GM_10380"
     tRNA            complement(1384..1468)
                     /locus_tag="AC16GM_10380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Leu,seq:tag)
     gene            1601..2149
                     /locus_tag="AC16GM_10385"
     CDS             1601..2149
                     /locus_tag="AC16GM_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924114.1"
                     /translation="MNLQKNKFIYIISCVFIVATQTQSFPQSAQAIDPWQHWRFLLGE
                     WVATGTGQPGEGVGHFSFTYDLDKKVLVRKSRVDYPPKPGEKTGLSHKDLLIVYQPPG
                     DSLFRAVYFDNEGHSINYAASFPETTLNAATLESEPSQKGPRFRLAYEMNADGTLQVS
                     FSIALPGESFKIYTQGIAHRKP"
     gene            complement(2299..3864)
                     /gene="hutH"
                     /locus_tag="AC16GM_10390"
     CDS             complement(2299..3864)
                     /gene="hutH"
                     /locus_tag="AC16GM_10390"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR2924115.1"
                     /translation="MSSIDGEHLTLDQIVAVARQGERVALSPKAKKKMAAARRFIDQK
                     VARGETIYGVTTGVGSFADTVLERSQAEKFQENLILSTAAGVGAPLAEEEIRALMLLS
                     LNCQAKGHSGSRVEVPLLLTGMLNRKVHPVIPSQGSVGASGDLAPLAHMALVLIGQGE
                     AVYRGRTYPGAKALKRAGLKPIRLREKEGLALINGTHLMTGLGMLALTDAIALVKQAD
                     VVGALTCEAVLARSAPFDARVHRLRPHPGQTATAGNFRKLLKGSRLIDAELPAPAMVQ
                     DAYSIRCIPQVHGAVRDAMAYVRSVLEIEINSTTDNPLIFANDGAVLSAGNFHGQPVA
                     QALDHLSLALTTLGNISERRIAKLLDKNHNNGLPAFLIPNGGLNSGLMVLQYTAAALA
                     SENKVLCYPASADTIPTSANQEDHVSMGPIAARHARQIAVNVANILAIELFCAYQALG
                     LRLSGEAEPTLNAQALSPAAAQVRKLLRKETGLPEVFLKDENFGSYQGNLRALLVNER
                     LAAQVERVCGKLV"
     gene            4264..5580
                     /locus_tag="AC16GM_10395"
     CDS             4264..5580
                     /locus_tag="AC16GM_10395"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR2924116.1"
                     /translation="MKGKTALLALVIALVIGGCSRQNSIGPSPAETQLAGTDLRTLVL
                     TSATDPRLPAPMAEAEGATADDWEMIKSIGSISTPTLFVLTLPNPVVYWNQLTTELGR
                     ARGLPPPRFARGYALTQVAIYDALVAGRVITRRGLADNALAAGAASEVSFYLFPLDSM
                     HIKEAIANQVDLRHPDLRTLRSWFLGKLVGRLVVIYGQHDGSDAVFPGPQPTGDCIWT
                     GVNPVLPMCGTWKTWITTSGAEFQPEPPYPCGSAQDLLEVEQVYDASLVRTPEQIAIV
                     HKWADLPPPTIWNGYLNDRLISNDWSNLRSARAHAFLNVTMYDAFVSCWRTKYITYTW
                     VARPFQRLAGRVPAFTTVITTPNFPTYTSGHSTISAAAAVVMGELFPGEQAFFNAEAE
                     EAALSRFWGGIHFHHDDNQGLVVGRQIGEKTVRIMRHLGIPVLAGE"
     gene            complement(5636..6670)
                     /locus_tag="AC16GM_10400"
     CDS             complement(5636..6670)
                     /locus_tag="AC16GM_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR2924117.1"
                     /translation="MLDEASRALARLAGLGERLPNPRLLIAPYLRREAVLSSRIEGTQ
                     TSLSDLFLFEAARSQPANGSDVKEVLNYVLALEHGLRRIKELPLSLRLVREIHGKLLE
                     GVRGQEKQPGSFRTIQNYIGPPGCKEEEATYVPPPVTELHRLLDKWEKFLHDDSGIPP
                     LIQCGILHYWFEAIHPFFDGNGRVGRLLIILFLCAREHLSQPQLYLSAFFEKHRGEYY
                     DRLLAFSRDGDWKNWLLFFLRGVAEQSKDAVERMQRMLSLQEKYRNRLDEAKANANVL
                     RLLDQLFRNPYTNIPRAAKQLRVSYLTAQAAISFLGRQGIVQEVTGKKRYRIFCAREL
                     LRTIEVDLTT"
     gene            complement(6979..8931)
                     /gene="gyrB"
                     /locus_tag="AC16GM_10405"
     CDS             complement(6979..8931)
                     /gene="gyrB"
                     /locus_tag="AC16GM_10405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR2924118.1"
                     /translation="MPDVLDKRNVVIGHHYDATTIQVLKGLEAVRRRPAMYIGDTGTR
                     GLHHLVYEVVDNSVDEALAGFCDQIKVAIHKDSSVTVEDNGRGIPVDIHPTQKRPALE
                     VVMTMLHAGGKFDHQSYKVSGGLHGVGVSVVNALAEWCEVEVARDGQVFFQRYEQGNP
                     KGDVKVTGKRQKTGTITTFKADPEIFGKVEYSYDVLASRLRELAFLNANLQIDLADER
                     TGKKETFKYKGGVAAFVAYLNENKQPLFSKPLYFHKEKEGVDVEIAIQYNDSYVENLF
                     SYVNNINTIEGGTHLVGFKTALTRTINNYAEKNGFLQKVDFSIIGEDVREGLTAVISV
                     KVPDPQFEGQTKTKLGNSEVKGIVETITGEGLASALEETPAVARKIVEKTLEAARSRE
                     AARKAKELVRRKSALENSPLPGKLADCSLDDPEQCEIYIVEGDSAGGSAKQGRDRRFQ
                     AILPIKGKILNVEKASQDRVFSNEEIRAIITALGTGVGTGDGNGDTDTFDLSKARYGK
                     VIIMTDADVDGSHIRTLLLTFFFRRMKALVEQGKVYIAQPPLYRLKKGNTEMYAYSDD
                     ERDRLMERLGRQGVGVQRYKGLGEMNPDQLWKTTMDPETRTLKQVSLEDAAEADHIFA
                     ILMGEEVEPRRKFIEENARYVKNLDV"
     gene            complement(8998..9285)
                     /locus_tag="AC16GM_10410"
     CDS             complement(8998..9285)
                     /locus_tag="AC16GM_10410"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR2924119.1"
                     /translation="MEKPELLSTILTQRLAALGLTKRVKAHQAVTLWPKVVGEKIAAE
                     SQAVRIEEGKLVVRVPKSVWRQELINLKPRLIAKINAVIGQAVVGDIFFTG"
     gene            complement(9444..>10524)
                     /gene="dnaN"
                     /locus_tag="AC16GM_10415"
     CDS             complement(9444..>10524)
                     /gene="dnaN"
                     /locus_tag="AC16GM_10415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR2924120.1"
                     /translation="RSTLPVLSNILIEAQDGKLKLSATDLEMSVTTILSANVTKKGVT
                     TVPAKTFGDIIRELPEVQVEVLVTDTRMEIKAEKGTYRLSGVPAEEFPKLPEFNGSKE
                     VKVPAQDLVQMVTKTVFAASKDETRPALNGVLWQMSGDKMIMVATDGHRLAKIGVENK
                     KLKGLPGNVIVPPKVLNMLERLVGDQEKEIGLVFSDSHIVFNLGDSILTSRLIEGPYP
                     NYEQVIPKNNDKRLSVPRDQMTAAVRRVAILSNSLTHQVKLTLKKSQMELSASNADVG
                     GDAREVIPCSFSGDEMEIGYNAQYVLDTLKQFDSDEVVFDLATPVSAGTIHAGSVPTR
                     AEEKKPADAPQADYFCLLMPLRLTE"
BASE COUNT         2190 a         3024 c         2770 g         2540 t
ORIGIN      
        1 cgatgcacct gcctgcgtta cgtgatcagt ttactcgttt tctcttttcc cgtctgcttt
       61 gttccgcttg ccgtttcttt tgccacgggc gtctccgaca cttcggagtg tttcaatatg
      121 tggtcgatga ctttctgctc taagatcgtc tcccgcacgt cttttatttt gccgcttttg
      181 gctaaagtct cctgcgcttc ttcgggcgtg atctggtagc gggcggcaaa gcgtgaaagc
      241 caggcgttgg tatcttcttt cgtcggcgcg agattttcct tttcggcgat ctggcgcaac
      301 agaatttccc attgaatcag ctcttcgccc aatggccggt aactctcccg cacctgtttt
      361 tcatcgaagt tctgataatt cttccggaaa tcggccgcca cgccgtcaag ataggtggcg
      421 agaagcgatt gcggtacggg aaagtgattt tgctccacca ccgtgtgccg cacctgcgcc
      481 cggaggcggg attcggcctc gcgctccatc tcggccgtca gcgtttgctg tactttctcc
      541 cgcatatccg caagcgcggt gaacccctcc tgcttggcaa actcgtcatc cagaggcggc
      601 aggatttttt ccttgacctc tttcgccagc acccagtagc ggacccggtt gcccgcatat
      661 gcctgttcgc tgaaatcggc ggggtagttc acgtcaaact ctttgatttc ccccacccgc
      721 atcccgacta tttctttggc aaaatcgtcc aagtccctgg tctgttctaa atcaatcgcc
      781 gcattctcct ttttgaacgg cgggatccgg ttctcccggt cggagagttt ctcaattgtg
      841 gcgacgacca cgtctccgga ggccgcggcc cgcgccaccg gcttgagttg ggctttcctt
      901 tcccgttggc gggtcagaaa ttcggtaacg tccgcatcgg tgatctcgta cttcggcttg
      961 ctgaatttga agccggtgta ctttttcagt tcagccaccg ggcgaatctc aaactccgcc
     1021 gcaaagcgca ggggctggcc gttggagaac tgaacgtctt tcacctccgg ctcggtgatg
     1081 gggtggaggt cagtctgcgc caccgcctcc cggtaggcct gaggcaccag ttcttccaac
     1141 acttcctgcc gggccagccc ggcgaagcgt tctttgacca aatccaacgg cgctttgccg
     1201 ggccggaagc cggggatttt ggctttggct ttgaactgct cgtaggtggc cgcaaactgg
     1261 gcagcgatcc gctccggcgg cacttcgatt tccagccgcc tgcgccagct gccctggtcg
     1321 gtcactttta ctttgatcgt gctggctgaa acaatttccg gcattttgga ttggctctat
     1381 ctatgcgaaa ggggggattc gaacccccac cccttttaag ggactggatc ctaagtccag
     1441 cgcgtctgcc aggttccgcc actttcgcgc aattatacta tataaatctg agtgtagggg
     1501 aagtcaagaa aatcgatctg ttgacagtaa aatccacttc agctatagta cgtcggccaa
     1561 aaactgtttc ccatcttcaa ccttgaaaag gagtactacc gtgaacctgc aaaaaaataa
     1621 attcatttac atcatctcgt gcgtgttcat agtagcaaca cagacacaga gtttcccgca
     1681 gtccgctcaa gcgattgatc cctggcagca ctggcgattt ctcctcgggg agtgggttgc
     1741 caccggcacg ggacagccgg gggaaggcgt cgggcatttt tccttcacgt acgatttaga
     1801 caaaaaggtt ttggtgcgca agagccgcgt ggattatcca cccaaacccg gcgaaaagac
     1861 cggtttatcg cacaaagatc tcctgatcgt ttaccagcca cccggagatt cgctcttccg
     1921 agctgtttac ttcgacaacg aaggccacag catcaactat gcggcgtcgt tcccggaaac
     1981 aactcttaac gcagccacac tggaaagcga accgtcgcaa aaaggaccgc ggtttcgcct
     2041 ggcgtatgaa atgaacgcgg acggtacctt gcaggtttca ttttccatcg cgcttcccgg
     2101 cgagtccttt aaaatttaca cacagggcat cgcgcaccga aagccctaac tttgataccg
     2161 ggcggaaaac ggctgctttc ttgaccgacg ttcccaacag ataatctgct tcaggtgacc
     2221 tcttctctga ccccccaacc tacaaagagg agctgatggt tttccctccc cttggagggg
     2281 aggacgaggg aggggtcgtc acaccagctt tccgcacacc ctttcgacct gcgccgccag
     2341 cctctcgttg accaaaagcg cccgcaggtt accctgataa ctgccgaagt tttcatcctt
     2401 caagaaaact tccggcaatc cggtttcttt ccgcaacaac ttccgcacct gcgctgccgc
     2461 cggcgagagc gcctgtgcat tgagagttgg ctctgcctcg ccggagagcc gcagacccag
     2521 cgcctggtac gcgcagaaaa gttcgatcgc taagatattg gccacgttca ctgcaatttg
     2581 ccgggcgtgg cgggccgcaa tcggccccat gctcacatgg tcttcctgat tggcggaggt
     2641 ggggattgta tcggctgaag cggggtaaca gaggactttg ttttcggaag ctaaagccgc
     2701 ggcggtgtat tgcagaacca tcaggccgga gttcaacccg ccgttgggaa tcagaaatgc
     2761 gggcaggcca ttgttgtgat ttttgtccaa gagtttagca atccgccttt cggagatgtt
     2821 ccccagcgtg gtgagcgcca gactcaaatg gtcgagcgcc tgcgccaccg gctggccgtg
     2881 aaaattgccc gccgaaagaa ccgcgccgtc gttagcaaaa atcaagggat tatcagtcgt
     2941 ggagttgatt tcaatttcca gaacgctccg gacatacgcc atcgcatcac gcaccgcccc
     3001 gtgcacctgc ggaatgcagc ggatcgaata ggcatcctgc accatggctg gcgcgggaag
     3061 ctcagcgtcg atgagccggc tccccttcag cagtttgcgg aaatttccgg cggtggccgt
     3121 ctggcctgga tggggtcgta gccggtgcac ccgggcatca aatggagcac tacgagctaa
     3181 aaccgcctcg caggtcaagg cacccacaac gtcggcctgc ttgaccaacg cgattgcgtc
     3241 agtcaaagcc aacatcccca gcccggtcat caggtgggtg ccattgatca aggccaggcc
     3301 ttctttttcg cgcagtcgga ttggcttcag ccccgctctc ttaagcgcct ttgctcccgg
     3361 ataagttctg ccgcgataga ccgcttctcc ctggccaatc aacactaacg ccatatgcgc
     3421 cagaggcgcc aggtcgccgg aggcgcccac ggagccttga gagggaatga ccggatgcac
     3481 cttccggttc agcattccgg tcaacagaag gggaacttcg acccgactgc cggagtgtcc
     3541 tttcgcctgg cagttgaggc tgaggagcat cagagcgcga atctcttcct cggccagagg
     3601 cgcacccact cccgccgcgg tggagaggat taagttttcc tggaactttt ccgcctggct
     3661 ccgctccagg accgtatcgg caaaagaacc gacgcccgtg gtgacgccgt aaatcgtctc
     3721 ccctctggcc accttctggt cgataaaccg ccgcgcagcc gccattttct ttttcgcctt
     3781 cggggatagc gccactcttt ccccctgccg ggcgacagcg acaatttgat ctaacgtgag
     3841 atgttcgccg tctatcgaag acatggtgag ggaatttata ctattttaga aaaagaaagg
     3901 agggaaaaac aaatcgatcg aggcgaaggg cacttctaaa cttcagtaaa cagtaccaac
     3961 gaataatctc gtattcgcgc tttgtgcaat accgatgttc ccgtgcgcaa gttattgcat
     4021 caaatacgtt ttggaattcc tgaaaacttt aattctcagc cgcttagtcg gttcggtcaa
     4081 taagataatc gtaagtggca cttccactta aaggccgtaa gaagcatccc tctaaccgaa
     4141 aagatacagg tattgaaatt gctgcgattc gtttatatct tagtcttttc gcaattataa
     4201 aagaggagtg cccgtctcaa tgctgcaaaa cagtttggca ccactactgc agaggaggaa
     4261 acgatgaaag gcaaaaccgc actgctcgcg ctcgtgatcg ccctggtgat cggcggttgc
     4321 agcaggcaga actccatcgg cccatctccg gccgaaacgc agttagcggg aaccgatctg
     4381 cgcacccttg tacttacctc ggccaccgac ccccgcctgc cggcgccaat ggccgaagcg
     4441 gaaggagcta ccgccgacga ttgggagatg ataaaaagca tcggctccat ttccacccca
     4501 accttatttg tccttaccct ccccaatcca gtagtttact ggaaccaatt gaccaccgaa
     4561 ctgggacggg caagaggatt gccgccgccc cgttttgccc gcggatatgc tttgacccag
     4621 gtagccatct acgatgctct ggtagcggga cgtgttatca cccgccgcgg tttggcggat
     4681 aacgccctgg cggccggtgc tgcctcggaa gtgtcgttct atcttttccc gctggattcg
     4741 atgcacatta aagaagcgat agcaaaccaa gtggacttga ggcatcccga cctgcgaact
     4801 ctgcggagtt ggtttttggg taaactggta ggcaggctgg tcgtgattta tggtcaacat
     4861 gacggctcgg atgccgtttt tcccggtccc cagccgaccg gcgattgcat ctggaccgga
     4921 gttaatccgg ttttgccgat gtgcggaacg tggaaaacct ggatcaccac ttccggtgcc
     4981 gaatttcagc ccgagccacc ttatccctgc gggtcggctc aggatcttct tgaggtggag
     5041 caggtgtacg acgcgtctct cgttcgcact cccgagcaga tcgccatcgt gcataaatgg
     5101 gccgatctcc ctccgccgac catctggaac ggttatttga acgaccggct tatcagcaac
     5161 gattggtcga atctacgttc ggcccgggcg cacgcctttt tgaacgtgac gatgtatgat
     5221 gcttttgttt cctgctggag gaccaaatac atcacctaca cgtgggtggc gcggccgttc
     5281 cagcgcttag ccggccgggt gcccgctttt acgacggtga ttacgacccc caatttcccg
     5341 acctacactt ccggccattc caccatctcc gcggcggcgg ccgtcgtgat gggtgaactc
     5401 ttccccggag aacaggcttt cttcaacgcc gaagccgagg aagcggccct ttcccgcttc
     5461 tggggcggca ttcacttcca ccacgacgac aaccagggtc tggtggtggg acggcagatc
     5521 ggcgagaaga cggttcggat catgcgccac ctgggcatcc ccgtactggc tggtgaatag
     5581 aatttctgtg aaaacttgaa cgggtgtggg agcactcctg cacccgtttt tgtttctacg
     5641 tcgtcagatc cacctcaata gtccgcagaa gttctcgggc gcagaagatc cgataccgct
     5701 ttttgccggt tacttcttgg acaatccctt gacgcccgag gaaactaatc gctgcttgag
     5761 ccgtgaggta cgacaccctt aactgtttag cagctcgcgg aatgtttgtg taaggattcc
     5821 gaaaaagttg atcgagaaga cggagcacat tggcatttgc ttttgcttca tcgagacggt
     5881 tacggtactt ctcttgaagc gaaagcattc gttgcatccg ctcaacggca tccttagatt
     5941 gctccgcaac accccgaagg aaaaacaaca gccaattctt ccagtcgcca tcacggctaa
     6001 acgccaaaag tctatcgtaa tattctcccc ggtgcttctc aaagaatgca ctcaagtaaa
     6061 gctgaggttg tgatagatgc tcacgtgcac acaaaaatag gatgatgagg agccgcccga
     6121 cacgaccgtt cccgtcgaaa aaaggatgaa tcgcctcgaa ccagtaatgg aggattccac
     6181 actgtatcag cggcggtatt cccgaatcgt cgtggagaaa cttctcccat ttatcgagaa
     6241 gtctgtgcaa ttctgttacg ggtggaggaa cgtaggtggc ctcctcttcc ttacatcccg
     6301 gcggaccaat atagttttga atggtacgaa aggaaccggg ctgtttttct tgaccacgca
     6361 ccccttccaa aagtttgccg tgaatttctc tgacgagtcg aaggcttagc ggaagttctt
     6421 taattcgtct tagtccgtgt tcaagcgcca gtacgtagtt tagaacttcc ttcacgtcgg
     6481 aaccatttgc cggttgactc cgggcagctt caaataaaaa gagatcagag agagaagttt
     6541 gggtaccttc aatgcgagaa ctcaataccg cttcgcggcg tagatacgga gcgatgagca
     6601 gacgtggatt gggcagtctc tctcccaaac ccgcgagtct cgccagtgct cgactggcct
     6661 catccaacaa aaaaactaag gcgggatcat actctaaggg gggtggcaat ttttcgggta
     6721 cgaacgccca gcatccacga ggcgttagta ccattcttcc gataggactg ttttgaaatt
     6781 caaggtggtt cattattctt gttcaaaatg cctaaaaatt tacttgttac aataacctgt
     6841 cttatttaag aaaatagccc ttttcttaaa taagagcaat ggctatttag gtttccctaa
     6901 ataaaaaagt gtgaaagaga ttgcaggtct tttaacgatc gggggtgcgt aatcctcctc
     6961 tgtatctctt tggggtagtt atacatccaa attcttcacg tatcttgcat tttcctcgat
     7021 gaacttccgc ctcggctcaa cttcttcacc cataaggatg gcaaatatgt gatcggcttc
     7081 ggcggcatct tcgagggaga cctgtttcaa cgtccgggtt tccgggtcca tggtggtctt
     7141 ccacaactgg tccggattca tttcgcccaa ccccttgtag cgctggacac ccaccccctg
     7201 ccggcccagc cgttccatca gccgatcgcg ctcatcgtcg gagtaggcat acatctcggt
     7261 attgcccttt tttagtcgat acaagggcgg ctgggcaatg tagactttcc cctgctccac
     7321 taaggccttc atccgacgga agaagaacgt caggagcagg gtgcgaatgt gggagccgtc
     7381 cacgtcggca tcggtcatga tgatcacctt gccgtagcgg gctttggaga ggtcaaaggt
     7441 atcggtgtcg ccgttgccgt cgcctgtccc caccccggtc cctaaagcgg taatgatggc
     7501 gcggatttct tcgttggaga aaacccggtc ctggctcgcc ttctcgacgt ttaagatttt
     7561 tcccttgatc ggcagaatcg cctggaagcg ccggtcgcgt ccctgcttgg ccgagccgcc
     7621 agctgaatcg ccttcgacga tgtagatttc gcactgctcc ggatcgtcca acgaacaatc
     7681 ggccaacttg ccgggcagag gcgagttttc taaggccgat ttgcgccgga ccagctcttt
     7741 ggcttttcga gccgcttcgc gggagcgtgc cgcctccagt gttttttcaa cgattttgcg
     7801 ggccaccgcg ggggtttctt cgagggcgct ggctaaaccc tccccggtga tcgtctcgac
     7861 aatccccttc acctcactat tgcctaactt ggtcttggtc tggccttcga actgcgggtc
     7921 gggcaccttc accgaaatca ccgcggtcag cccttcgcgc acgtcttcgc cgatgatgga
     7981 gaagtcaacc ttctgcagaa agccgttctt ctcggcgtag ttgttgatgg tgcgggtcaa
     8041 agcggtcttg aagccgacta aatgggtccc gccctcaatg gtattgatgt tgttgacgta
     8101 ggagaaaaga ttctcgacat aggaatcgtt gtactgaatg gcaatttcga cgtccacgcc
     8161 ctctttttct ttgtggaagt aaaggggctt ggagaaaagg ggctgtttgt tttcgttcag
     8221 gtacgctacg aaggccgcca ccccgccttt gtacttgaat gtttctttct tcccggtccg
     8281 ttcgtcggcc agatcgatct ggaggttggc gttgaggaag gccaattccc gcagccggct
     8341 ggctaagaca tcgtaactgt attcgacctt gccgaaaatt tccgggtcgg ccttgaaggt
     8401 cgtaatggtg cccgtcttct gccttttgcc ggtgaccttg acatcccctt tggggttccc
     8461 ctgctcgtag cgctggaaaa agacctgccc gtcgcgggcg acctccacct cgcaccattc
     8521 cgcaagagcg ttgactaccg agacgccgac tccgtgcaat ccgccggaga ctttgtagga
     8581 ctggtgatcg aacttgccgc cggcgtggag catcgtcatc accacctcca gagccggccg
     8641 tttctgggtc gggtggatat cgaccggaat cccccggccg ttgtcctcca ccgtaacgct
     8701 tgagtccttg tggatggcga ctttgatctg gtcacagaaa ccggccaagg cctcgtccac
     8761 ggagttatcc accacttcgt agactaagtg gtgcagaccc cgcgtgccgg tatcgccgat
     8821 gtacatcgcc ggccggcgcc ggaccgcctc caggcccttc agcacctgga tggtggtggc
     8881 atcgtagtgg tggccgatga ctacattcct cttgtcaagg acatcgggca ttgattcccc
     8941 tctcgtagcc gagtcgtacc gatcctgccg caccggatga tttttctccc agcaacgtca
     9001 tccggtgaag aatatgtccc ccaccactgc ctgcccgatg acggcattga tcttggcaat
     9061 cagtcggggc ttgagattga tcaactcctg ccgccagacc gacttcggga ccctgaccac
     9121 caatttccct tcttcgatcc gcactgcctg ggattcagcg gcgattttct ccccgacgac
     9181 cttcggccag agcgttaccg cctggtgggc tttaacccgt ttggtcaacc ccagagctgc
     9241 cagccgttgg gtgagaatag tgctcaaaag ttccggtttt tccataaagg gataacgacc
     9301 gttccggtcg tatcttcacg tttataaaat ctacggaaat tggcggcttt acgcaagaag
     9361 aaccggggcg gggagacccc gcccctacgc acccgggagg gcgacccgta cgggtcgccc
     9421 agttactgat ccgttcggga cagtcactcc gtcaaccgca acggcattaa caagcaaaaa
     9481 taatctgcct gaggggcatc ggcgggtttc ttctcctcgg ctcgggtcgg gaccgatccg
     9541 gcgtggatcg tacccgccga gaccggcgtg gctaagtcaa acaccacctc gtcggaatca
     9601 aactgcttga gtgtatccag tacgtactgg gcgttgtagc cgatctccat ttcgtctccg
     9661 ctgaacgaac aggggattac ctctctggca tcccccccca cgtcggcgtt ggaggccgaa
     9721 agttccatct gcgatttctt cagggttaat ttcacctgat gcgtgagtga attggagagg
     9781 atagccaccc gccgtaccgc ggcggtcatc tggtcccgcg gcacgctcaa gcgcttgtcg
     9841 ttgttcttcg gaatgacctg ctcgtagttg ggatacgggc cttcaatgag ccgtgaggtc
     9901 aggatagaat cgcctaagtt gaacacgatg tggctgtcgg agaagaccag cccaatctct
     9961 ttttcttgat ccccgacgag gcgctccagc atattcaaca cttttggcgg aacaatcacg
    10021 ttaccaggta agcctttgag ttttttgttt tccaccccga ttttggccag ccggtggccg
    10081 tcggtggcca ccatgatcat tttgtcgccg gacatttgcc agaggacacc gtttaaggcc
    10141 ggtcgcgtct cgtcttttga ggcagcaaag accgttttcg tgaccatttg caccaaatcc
    10201 tgcgcgggaa ctttcacctc tttgctgcca ttgaattccg gtaatttcgg aaattcttca
    10261 gccggcactc cggagagccg gtacgttccc ttttcggcct taatttccat acgggtgtcg
    10321 gtcaccaaaa cctcgacttg aacctccggt aattcacgga tgatatcgcc gaaggtctta
    10381 gcgggaacag tcgtcacccc cttcttcgtc acattggccg aaagaattgt cgtgacggac
    10441 atttccagat cggtcgctga aagtttcaat tttccgtctt gagcttcaat gaggatattg
    10501 gaaagcaccg gtaaggtaga ccgc
//
LOCUS       DBNGZD010000402         3751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_272167, whole genome shotgun sequence.
ACCESSION   DBNGZD010000402 DBNGZD010000000
VERSION     DBNGZD010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_272167"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="AC16GM_10420"
     CDS             complement(<1..493)
                     /locus_tag="AC16GM_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924121.1"
                     /translation="MNVETLIAAILTLAIFSFLYRDNPLYKFAEHLLVGISVGYSVMV
                     AVFSTIGPKLFSPLGHRPTPAAFVSLLLALFLLARLVPRWAWVSRLALSLMIGAGAGL
                     AIPAMLQARILSQLQASLLPLTSLNNILIVVGIVTTLLYFYFSREQKGVWKGTAVVGT
                     YFLM"
     gene            complement(490..1302)
                     /locus_tag="AC16GM_10425"
     CDS             complement(490..1302)
                     /locus_tag="AC16GM_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924122.1"
                     /translation="MTFFRFDRRYLFGFMALAVAVPLLFPLHLPNEVTPDVRHFYNLI
                     DSLPAGSVVLVSFDHEASTIVEVRPPATAVIRHLFQKDIKIIGVSLLAEGTGIGRELL
                     LRLGREYGKQEGTDFAFLGFRPQYAATISGLGEDIRRVFPTDYRDRPLDSLPLFSSVR
                     NLADLALVISVADGDLPATWVDYAVARYHQKVAAAVTAVMATTYYPYLNSGQLVGLVS
                     GLKGASEYELLLHKPGGGVRGMDAQSVAHLALLLLIGLGNLAFLASRKRAAR"
     gene            complement(1299..1808)
                     /locus_tag="AC16GM_10430"
     CDS             complement(1299..1808)
                     /locus_tag="AC16GM_10430"
                     /inference="COORDINATES: protein motif:HMM:NF019932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR2924123.1"
                     /translation="MTFLVLLLVIVNLALLYLYLKKRGTPRWEREKSEIQQVMKEITQ
                     ALRAKELKAISRYLADDFTAFLDSPYRCDSRKAYLAELSALFEHGGIEERSFRQEKVQ
                     VINENCAVVTYHFTEKGTRKDKLYERQGKATEVFRRQPEGWVAVHFHHTLVDETKKPF
                     HFPMAEPGV"
     gene            complement(1810..2433)
                     /locus_tag="AC16GM_10435"
     CDS             complement(1810..2433)
                     /locus_tag="AC16GM_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924124.1"
                     /translation="MSRRLPLWIAFGFGVLLLVQYFFRHPVATGFYRNTLDWMQVVAV
                     FSLIVGATALAGMHLKAVAARSRGWGYNLVTLAGLGGMIFCGALWGIDEHSPFTWAFL
                     NLQVPLQSTMFSLLAFFVASAAYRGFRARSWPAAVLLLAAAIVIMGRVPVGAQILAGL
                     PQAADWVLNVPSMAARRGILIGIGLGAIATSLRVILGIERTYLGKEG"
     gene            complement(2430..3311)
                     /locus_tag="AC16GM_10440"
     CDS             complement(2430..3311)
                     /locus_tag="AC16GM_10440"
                     /inference="COORDINATES: protein motif:HMM:NF042826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR2924125.1"
                     /translation="MGKRSIHRRWSSVNFFFLFFLAFTLAGEPAGSAVSESMGFQTDR
                     LPILVGFLVCAAVLLLTLTLGRKRKGYVRPIPGLAAVDEAVGRATEMGRPLLFTSGTG
                     EVDRMATLAALSILGTVAKTAAEHDTRLIYPNNDPVVAAVAQEVVKESCLRAGRPEAF
                     QSEDIFFVTKSQFGYAAAVDGIMVRQKPAACLFFGTFEGESLIFAETGNGIGAIQIAG
                     SDSTIQLAFFLVACDYTLIGEELFAASGYLSGDPVIVASLRGQDYLKLAVIGFLLLGA
                     LAATLHIADLAKLLGGG"
     gene            3438..>3751
                     /locus_tag="AC16GM_10445"
     CDS             3438..>3751
                     /locus_tag="AC16GM_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR2924126.1"
                     /translation="MQMKFRFWLVFVVAGYATVLMSACGRQQAEPVTLTWWQFRTDRE
                     TRPLIDSLVQKFEKRYPKIKVAVTDLTWEAGHDKIAIAFGADAAPDVIEFGSDWVPEF
                     ITA"
BASE COUNT          886 a         1110 c         1097 g          658 t
ORIGIN      
        1 ccatgagaaa gtaggtgccc accactgccg tccctttcca gactcccttc tgctcgcggg
       61 aaaagtaaaa gtataggagg gtggtgacga tgcccacgac gatcaaaatg ttgttgaggg
      121 aggtcaaggg caaaagcgag gcctggagct gggagagaat gcgggcctgg agcatggccg
      181 gaatggcgag cccggcgccc gccccgatca taagcgaaag cgccagccgg gagacccacg
      241 cccagcgcgg caccagccgg gccagaagga aaagggctaa gaggagcgaa acgaaagcgg
      301 ccggggtggg gcggtgcccg aggggcgaaa acagctttgg cccgatggtc gaaaaaaccg
      361 ccaccatgac cgaatagccg accgaaatgc cgactaagag gtgctcggca aatttgtaga
      421 gaggattatc ccggtacaga aaagaaaaaa tggccagagt aagaattgcg gcgatcagtg
      481 tctccacgtt caccgggccg ccctttttcg actggcgagg aaggccagat tccccaggcc
      541 aatcaagagg agcagagcca ggtgggccac cgattgtgcg tccatcccgc gcaccccgcc
      601 gccgggttta tgcagcagga gttcatactc gctcgcccct ttgagcccgc tgacgagccc
      661 gactaactgg ccggagttga ggtaggggta gtacgttgtc gccatcacgg cggtgactgc
      721 ggcggccacc ttttggtgat agcgggcgac ggcgtaatcg acccaggtgg cgggcaggtc
      781 gccgtctgcc accgaaatga ccagcgccag atcggcgaga ttcctcacgc tcgaaaataa
      841 cgggagcgaa tccagcggac gatcccggta atcggtggga aagacccgac ggatatcctc
      901 tcctaagcca gaaatcgtcg ccgcatactg cggccggaag ccgaggaagg caaaatcagt
      961 gccctcttgt tttccatatt cccttcccag ccggagtaag agctcccgcc cgatgcccgt
     1021 gccttcagcc agaagcgata ccccaattat tttgatatcc ttttgaaaca gatggcggat
     1081 gaccgcagtg gctggcggcc gcacttcgac aatggtggaa gcttcgtggt cgaacgagac
     1141 taagactacg cttccggccg gaagcgaatc aataaggttg tagaaatgcc ggacgtccgg
     1201 cgttacctcg ttcggcaggt gcagaggaaa aagcaaaggc accgccaccg ccagagccat
     1261 aaacccgaag aggtagcggc ggtcgaagcg gaaaaaggtc atacgccggg ctcggccatc
     1321 ggaaagtgga agggcttttt ggtttcatcc actaacgtgt ggtggaagtg aaccgccacc
     1381 cacccttccg gctggcggcg gaatacctcg gttgccttac cctgccgctc gtacagtttg
     1441 tctttacgcg tccctttttc ggtaaaatga taggtcacca ccgcgcagtt ttcattaatc
     1501 acttgcacct tttcctgcct gaaactgcgc tcttcaatgc cgccgtgttc gaaaagcgcg
     1561 gagagttctg cgagatacgc cttgcggctg tcacagcggt agggcgaatc gaggaaggcg
     1621 gtgaagtcgt cggccaggta gcgactgatc gccttcagtt ccttggcccg cagggcctgc
     1681 gtgatttctt tcatgacctg ctgaatttca cttttctccc gctcccatcg gggagtgccg
     1741 cgcttcttga ggtagaggta gagaagcgca agatttacaa tcaccaatag aaggacaagg
     1801 aaagtcatat cacccttcct ttcccagata ggttcgctcg attcctaaga tgacacgcag
     1861 ggaggtggcg atggctccca ggccgatgcc gatcaaaatc ccccgccggg ccgccatcga
     1921 gggaacgttc agcacccagt cggctgcctg cggcagacca gccaaaattt gggcacctac
     1981 cggcacccga cccataatga caatcgctgc ggcgagcaag aggactgccg ctggccaaga
     2041 acgtgcccgg aaaccgcggt aagcggcgga agcgacgaag aacgccaaaa gggaaaacat
     2101 cgtcgattgc aagggtacct ggaggttcag aaaagcccag gtgaaaggtg aatgctcatc
     2161 gatgccccag agcgccccgc agaaaatcat cccgcccaga ccggccaaag tcaccagatt
     2221 gtatccccag ccccggctcc gcgccgccac tgctttcaga tgcattccgg ctaaagcagt
     2281 ggcaccgacg atgagcgaaa agaccgccac tacctgcatc cagtccaggg tgttccggta
     2341 aaagccggtg gcaaccgggt gccggaagaa gtactggacg agcaaaagca cgccaaaccc
     2401 gaacgcaatc cagagcggca gccggcggct catccgcccc ccagaagttt agcgaggtcg
     2461 gcaatgtgca acgtggcggc gagcgctccc aaaagaagga aaccgatcac cgccaatttg
     2521 aggtaatcct gcccgcgcag gctggcgacg atcaccggat caccggagag gtagccggaa
     2581 gcggcgaaca actcttcccc gatcagggtg tagtcgcagg cgaccaggaa aaaggccagc
     2641 tggatggtgg aatcgctgcc ggcgatttga attgcgccga tgccgttgcc ggtctcggcg
     2701 aaaatgagcg attccccttc aaaggtgccg aagaagaggc aggcggccgg cttttggcgc
     2761 accatgatgc cgtccaccgc cgccgcgtag ccgaactggc ttttggtcac gaaaaagata
     2821 tcctcgcttt ggaacgcctc cggccggccg gcccggagac aactttcttt tacgacttcc
     2881 tgcgctaccg ccgccaccac cgggtcgtta ttgggataaa tcaggcgggt atcatgctcg
     2941 gccgcggttt tggccactgt gcccagaatc gaaagcgccg ctaaggttgc catccggtcc
     3001 acttccccgg tgccggaggt gaacagaagc ggccgcccca tttcggtcgc ccgccccacc
     3061 gcttcgtcca ccgccgcgag ccccggaatg ggccggacat atcccttccg cttcctcccc
     3121 agagtcagcg tcaaaagcaa aacggcagcg cagacgagaa aaccgacgag aataggcagg
     3181 cggtcagttt gaaagcccat tgactcggaa acagccgacc cggccggttc tccagccaga
     3241 gtaaatgcaa gaaaaaagag gaaaaagaag ttgacgcttg accagcgccg gtgaatggac
     3301 cttttgccca aacgcagtac ggctctccct cctcctgacc gagagaaaac ttaagcggcg
     3361 gctgggatta agtcaacgaa atattttccc gttgacagcc gatacctgag ccaatagaat
     3421 ctctacccgg agcaccgatg cagatgaaat tccgtttctg gctcgttttc gttgtcgctg
     3481 gctatgcgac agttctgatg tccgcctgcg gacggcagca agctgagccg gtcaccctta
     3541 cctggtggca gttccggacc gaccgggaaa cccggccgct cattgacagt ttggtacaga
     3601 aatttgaaaa acgctacccc aaaatcaaag tcgccgtcac cgacctcacc tgggaggcgg
     3661 ggcacgacaa aatcgccatc gccttcggcg ccgatgcggc gccggacgtt attgaattcg
     3721 gttccgattg ggtccccgaa tttatcaccg c
//
LOCUS       DBNGZD010000403         5645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_827036, whole genome shotgun sequence.
ACCESSION   DBNGZD010000403 DBNGZD010000000
VERSION     DBNGZD010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_827036"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="ruvB"
                     /locus_tag="AC16GM_10450"
     CDS             <1..726
                     /gene="ruvB"
                     /locus_tag="AC16GM_10450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR2924127.1"
                     /translation="LTNLSEHEVLFIDEIHRLNHVVEEYLYGAMEEFALDIMIDRGPA
                     ARSVRLPLKKFTLIGATTRAGLLTSPLRARFGVVGRLDFYQPEDLFQIIQRSAKILSV
                     QIDEAGALEISSRSRGTPRVANRLLRRVRDFAQVESNGKITKAVAQDALARLDVDERG
                     LDEMDKRLIEVIISKFKGGPVGINTLAVAVGEEEDTIEEVYEPFLIQEGFINRTPRGR
                     FATETAYRHLGLLPDKQAQPRLF"
     gene            complement(632..1147)
                     /locus_tag="AC16GM_10455"
     CDS             complement(632..1147)
                     /locus_tag="AC16GM_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR2924128.1"
                     /translation="MTVGHQLQNADYHLKPAELKKVIYAPLLSFRDRTILKTFAQTGI
                     RRAELAALDVRDIDFERRTLEIRAGKGSKSRTLPLSEDLASDLRHLVGRRKTGPVFVS
                     QRGSFLAPRQLNWIVARAGELAGVKDPNPRARHLHIHTFRTTAAAPACPAEVLSADRR
                     FRWQSGPAASG"
     gene            complement(1335..1730)
                     /locus_tag="AC16GM_10460"
     CDS             complement(1335..1730)
                     /locus_tag="AC16GM_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924129.1"
                     /translation="MNSKIGIFRLVTALTVGVGLYGFSKVPDTTFTHTSNPDLVDTNS
                     NSSYYPDPSDANMLTDEPCPIDTVYLPLPCPSPETVLVVVHDTVFIGDERDGDDYIRW
                     PDDRHGIWPDDGRPDWWYDTTQTEDWNLC"
     gene            complement(1813..2385)
                     /locus_tag="AC16GM_10465"
     CDS             complement(1813..2385)
                     /locus_tag="AC16GM_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924130.1"
                     /translation="MKLKTAGVLLGGLLLLVAPAITLGQSGSYGDQVEDGIDMAVAPD
                     AIDPNPCDLNPCIGPPVEPVYPEPYFDWDGWDRLFRLDGEEPPDDPFNFDPDIEAEPL
                     VDNGPDQEGLSVADNSGGNTTDETMDNGANRAEPTHDTVAGGETGNGETAGGGDGNDP
                     GGGDGPDGPDGGDHDGGGGDDGGSDGGDVG"
     gene            2659..3840
                     /locus_tag="AC16GM_10470"
     CDS             2659..3840
                     /locus_tag="AC16GM_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2924131.1"
                     /translation="MKTLDKVSGLGGEAENIGRFLQDVRERKALSQSTVAERAHITQS
                     ELSKIERGKHLPNLDTLRRIVAALGETPLGIRALWAEIGDERTSVEVTPQDLKEGQNF
                     DHAKEEAKRLMYEGNLFHASKKIAVMERLAKTLDERVRAGLYAGYYAREAGFYADSDE
                     YFREAVKALTDVDEKSVLWDMYYVNHASVLTYTGNLPWAEMLLDKLMARNPGDNFYHR
                     WGVFERGRLALAREDAQKAKALFKSAIHHFDECREKDERLLPCHLGYFQLFLAKSMLT
                     SDEEKEKACMLLQHLMECWGPDENRPRNAEIYAEAALEYGLATNKEAFLEKAKDVAKK
                     NGLIRIVRIVQKHQPEDENRNNPKDGASRVPIILSSLAFFLLFSYFVLLPLAQAMCAI
                     E"
     gene            4087..5274
                     /gene="ispD"
                     /locus_tag="AC16GM_10475"
     CDS             4087..5274
                     /gene="ispD"
                     /locus_tag="AC16GM_10475"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR2924132.1"
                     /translation="MQKISAIVVAGGTGSRFGNGLPKQFARLGDRPILAHTLGKFQLV
                     EQVAEIVAVVPTEWESYCRDEIVTPFNLTKVARVVTGGATRQESVARGLAAVAPDTTT
                     VLVHDGVRPLVSPEKIAAVCDALEHSPACLLVLPVQETLKEGEGGAVRKTLERQGVYL
                     AQTPQGFHKSLLAQALAQAEKDHFTATDDAALVERLGVTPQIIAGEPTNLKITTSEDL
                     AFAERLFGLPVTQSRVGFGYDSHRLVAGRPLILGGVTIPFEKGLAGHSDADVLCHAFA
                     DALLGSLGLGDIGSHFPDTEEKWRGVSSLEILRQVAVKVAASHGEITSVDATVVAEKP
                     RLGPYIEEMREKIAQALGISATLVSIKAKTAEGLGSIGAGEAMATYAVAAVNQRKVEG
                     GRR"
     gene            5335..>5645
                     /locus_tag="AC16GM_10480"
     CDS             5335..>5645
                     /locus_tag="AC16GM_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR2924133.1"
                     /translation="MEAFSARAQELLNLATSYGVWWLVFCYVFLYLSAIVESLFPPYP
                     GDVVTFAGGYLASLGKLNFPLVFLATSLGSLSGLLLLYAIGQRHGRRLFQKEQSHWRT
                     PA"
BASE COUNT         1251 a         1471 c         1648 g         1275 t
ORIGIN      
        1 ttgaccaatc tttccgagca cgaggtgctc ttcatcgacg agattcaccg gttgaaccac
       61 gtcgtcgaag aatatctgta cggggcgatg gaggagttcg ccttagatat tatgatcgac
      121 cgcggcccgg cggcgcgctc ggtgcgcctg ccgcttaaaa aattcacctt aataggcgcc
      181 accacccggg ccgggcttct gacttcgcct ttgcgcgcgc gctttggcgt cgtggggcgg
      241 ctggatttct accagccgga ggatctcttc caaattattc agcggtcggc caaaattctg
      301 agcgtgcaaa tcgatgaagc gggcgccctg gaaatttcct cccgctcgcg ggggacgccc
      361 cgggtggcca accggctatt gcgccgggtg cgcgattttg cgcaggtgga gtcgaacggg
      421 aagatcacca aggccgtagc gcaggatgcc ttggcacggc tcgatgtgga cgagcgcgga
      481 ttggacgaga tggacaagcg gttgatcgag gtcataattt ccaagttcaa aggcgggccg
      541 gtggggatca acacgctcgc cgttgctgtc ggcgaagaag aggatacgat tgaagaagtg
      601 tacgagccgt ttttgattca ggaagggttt atcaaccgga cgccgcgggg ccgctttgcc
      661 accgaaaccg cctatcggca cttaggactt ctgccggaca agcaggcgca gccgcggttg
      721 ttctgaacgt atggatatgc aggtggcgcg cccgagggtt cgggtctttc actccggcca
      781 gttccccagc ccgggcaaca atccagttca gctgccttgg ggcaaggaag ctgcctcgtt
      841 gggatacgaa aacgggaccg gtctttcgtc tcccgaccaa gtgccgcaaa tcgctggcta
      901 aatcttccga aagcggcagc gtccggctct tggaaccttt cccggcgcgg atttcgagtg
      961 tccggcgctc gaagtcgatg tctcggacgt ccaatgctgc cagctccgcc cggcggatgc
     1021 cagtttgggc aaaagttttc aggatggttc ggtcccggaa ggacagaagc ggagcgtaga
     1081 tgaccttttt caactccgcc ggtttcaagt ggtagtcagc gttttgcagc tgatggccga
     1141 ccgtcatagc accttaccta aaagaagtga aaaacttatc atccggggca aggatagcgt
     1201 ctgcggccgg tagctgtcaa gccgaaagat agcacattct gtataaggta ggcaatgttt
     1261 gccgtaaatt aagaaagcct acgctgttaa atgcgtaggc tttcagtgcg ctggttacgg
     1321 tacaacagcg aggttcaaca aaggttccaa tcctctgttt gcgtcgtatc gtaccaccag
     1381 tccggccttc cgtcgtcggg ccagatgcca tggcggtcgt ccggccatct aatgtagtca
     1441 tccccatccc gctcgtcccc aataaaaact gtatcgtgaa ctacaacgag cacggtttcg
     1501 ggcgaaggac acggcaatgg caaataaacc gtgtcaattg ggcacggctc atctgtcaac
     1561 atgttcgcgt cgctcgggtc cggataatac gaagaattag aattcgtatc gaccaggtcc
     1621 gggttggacg tatgggtgaa cgttgtgtcc ggtaccttgg aaaacccgta aaggccaacc
     1681 cctaccgtca gtgcggtgac caatctaaaa atgccaatct tcgagttcat ctttgttttc
     1741 acctcctctc aagtttttgt ttgactaccc ggaaggtttt gccctccccc tgcgggtagt
     1801 cagttatttt agctaaccga catctcctcc gtcggagcca ccatcgtccc cgcctccgcc
     1861 gtcgtggtcg cccccatcag gaccatccgg cccatcaccc ccgcccgggt cattcccgtc
     1921 gcccccaccg gcggtctctc catttccagt ctcaccgccc gctaccgtat cgtgggtcgg
     1981 ctcagcccga tttgccccgt tgtccatggt ttcatccgtg gtgttaccac cggagttgtc
     2041 cgctaccgag aggccttctt ggtctggccc attgtcgacc aacggctctg cttctatatc
     2101 cgggtcgaag ttgaaggggt catctggagg ctcctccccg tcgaggcgaa acaaccggtc
     2161 ccacccatcc caatcaaaat atggctctgg gtaaaccggc tccactgggg ggccgatgca
     2221 cgggtttagg tcgcagggat tcgggtcgat ggcgtccggc gctaccgcca tatcaatgcc
     2281 atcttcaact tggtcaccgt acgaaccgct ctggcccagg gtgatggccg gggcgaccaa
     2341 aagcaagagt cctcccagta ggacgccggc cgtttttaac ttcatcatat tgctctccga
     2401 tctggggcct gtgtttttcc cagcgaaccc cggccccggt cgggtcaact ggctggctgc
     2461 gcgcatctgc ctattcatac caattcttta tacgaaccac tcagggggat tgggctataa
     2521 atcattatgt aacaatagaa tgggggctat gcgtggatta caaggtctgt gaatgctctc
     2581 cacgggtgtt caaacgtatt aattaatgga gagaaaatta tggagcggct aaaaccattt
     2641 aaggaaagga gaccaacgat gaaaaccctc gacaaagtta gcggccttgg aggggaagca
     2701 gaaaatatcg ggcgttttct gcaggacgtg cgtgaaagga aagcactttc ccagtcaacg
     2761 gttgcggaac gggcacatat aacccaatca gaactgtcca agatcgaaag ggggaagcac
     2821 ttgcctaact tagatacttt gagaagaatc gtagctgcac tgggcgagac tcctctgggg
     2881 atacgcgcgt tgtgggccga aattggggat gaacgtacaa gcgtcgaggt cacgccccaa
     2941 gaccttaaag aagggcagaa cttcgaccat gctaaggaag aggcaaaaag attgatgtat
     3001 gaagggaatc tttttcatgc atcgaagaaa attgcggtca tggagcgctt agctaaaact
     3061 ctggatgaga gggtgagagc agggctctat gccgggtact acgctaggga agccggattt
     3121 tacgcggatt ccgacgagta tttccgtgaa gccgtgaagg cccttacgga tgtggacgag
     3181 aaaagcgtgc tttgggatat gtactacgtg aatcacgcca gcgttttaac ctacacgggt
     3241 aacctacctt gggcggagat gttattggat aagcttatgg cccgaaatcc cggcgataat
     3301 ttttaccatc gctggggggt attcgagcgt gggcgacttg cgctcgcaag agaggacgcc
     3361 caaaaagcga aagctctttt taaatctgcc attcaccatt tcgatgaatg cagagagaaa
     3421 gatgagaggt tgctgccctg tcatttgggt tactttcaac ttttcctcgc gaagtcgatg
     3481 ctcacctcgg atgaagagaa ggagaaagca tgtatgctcc tacaacatct gatggagtgt
     3541 tggggaccgg acgaaaaccg cccacgtaat gcagaaatct atgccgaggc ggctctggaa
     3601 tatggacttg caaccaacaa ggaggctttt ttggagaagg caaaggatgt tgcgaaaaaa
     3661 aacgggctta tcaggatagt gcggattgtt caaaaacacc agccggaaga cgaaaacagg
     3721 aacaacccga aggatggggc cagcagggta cccataatac tgtcgtctct ggcatttttc
     3781 ttgttgttct catacttcgt gctattgcct ttggctcagg caatgtgcgc tattgaatga
     3841 ctcagaccag tgaaatgcgc tgaggggttt tcctctcagc gcttttttta ttagcctcta
     3901 caatacacca tgactgcaag gggtgggaag gatttttccg cgagcacgaa aaaggcggag
     3961 cgggggttgc ccgctccgct tcttcgctac cggcggcctt cgatcccgcc gtcggcgggt
     4021 attggtgctg gctttaactc tccttttgaa tttgttcttc cgcaaataag tgttttcttt
     4081 acctccgtgc agaagatctc ggcaattgtg gtagccggtg ggacggggag tcgttttggc
     4141 aacggcctgc caaaacagtt tgcccgctta ggcgaccggc ctatcttagc gcacacattg
     4201 gggaaatttc agttggtgga gcaggtcgct gaaattgtcg ccgttgtgcc cacggagtgg
     4261 gaaagctatt gccgggatga gatagtgact ccattcaacc tgacaaaagt tgcgcgcgtc
     4321 gtcaccggcg gggcgacgag gcaagagtcg gtggcgcgcg ggctggcggc ggtggcgccg
     4381 gataccacaa ccgttctggt gcatgacggc gtgcggccgc tggtgtcgcc ggagaaaata
     4441 gcagcggtct gcgacgcgct ggagcattca cccgcctgcc ttctggtttt gccggttcaa
     4501 gagacgttga aggaaggcga aggcggagcg gttcggaaaa ctttagaacg tcagggtgtt
     4561 tacctggcac agacgccgca ggggttccac aagagtttgc tggcgcaggc cctagcgcag
     4621 gcggaaaagg atcatttcac cgctactgat gacgcggcgt tagtcgaacg tctcggagtc
     4681 actccacaaa tcattgctgg ggagccgacg aatctgaaaa tcaccacgtc tgaagatttg
     4741 gccttcgccg agcgcctttt cggtctgcct gtgacacaaa gccgcgtcgg ctttgggtac
     4801 gacagccatc ggctggtggc gggacggcct ctgattttag gcggagtcac gattcctttc
     4861 gagaaggggc tggccgggca ctccgacgcc gacgtcctct gccacgcctt tgccgacgcc
     4921 ctgctgggca gtttgggctt aggcgacatc gggtcacatt ttccagatac ggaggaaaag
     4981 tggcgcggcg tttcgagtct ggaaattttg cggcaggtgg cggtcaaggt ggccgccagc
     5041 cacggggaaa tcacaagcgt tgatgcaacg gtagtggcgg aaaagccacg tttggggccg
     5101 tacattgaag aaatgcggga gaaaattgcg caggcactgg ggatttccgc cactctggtt
     5161 tccatcaaag ccaaaaccgc cgagggattg gggtcaatcg gtgctggaga agcgatggcg
     5221 acctatgcgg tggcggcagt aaaccaaagg aaggtcgaag gtggtaggag gtaggtgata
     5281 gggaaagact aagaatcttt ccctaccacc gatcacctat cacctataac ctgcatggaa
     5341 gcattttctg cccgcgccca agagttgctc aacctcgcca ccagttacgg cgtatggtgg
     5401 ctggtttttt gctacgtatt tttgtatttg agcgccatcg tcgagtcgct ttttccgccc
     5461 tatccgggag acgtggtgac ttttgcgggc ggatacttag cgtcactagg aaaattgaac
     5521 ttccccctag tctttttggc caccagtctg gggagtctct ccgggcttct gctcctgtac
     5581 gctataggtc aaaggcacgg acgccgactc tttcaaaagg agcaaagcca ttggcggacg
     5641 ccggc
//
LOCUS       DBNGZD010000404         1669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_132782, whole genome shotgun sequence.
ACCESSION   DBNGZD010000404 DBNGZD010000000
VERSION     DBNGZD010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_132782"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="AC16GM_10485"
     CDS             <1..821
                     /locus_tag="AC16GM_10485"
                     /inference="COORDINATES: protein motif:HMM:NF025229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2924134.1"
                     /translation="QFNLSFVVPKDISYGSKTAKISVYIAGGSTDGVGLLDSLTLAGA
                     PVAITDSLGPEIDLRFDGRPAADGDFVRADATLTGMLRDQNGINITGEPGHGIVLTLD
                     GNRQASYDLTDRFAYDPGSFQAGTVTFTLPNLASGAHTVELKAWDSANNSAVRRVTIQ
                     AASEGSLAIRDFLAYPNPFRDQTTFCYQLTRDVDQMKIMIFTESGRQIKTIEGPNQLA
                     GYHADLVWDGCDQRGDRVANGLYLCKAVAQGRVAAGTNAVTKTAEEFGKVLVVR"
     gene            861..>1669
                     /locus_tag="AC16GM_10490"
     CDS             861..>1669
                     /locus_tag="AC16GM_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924135.1"
                     /translation="MKRAVGMLTVAMLFVSFSYRQGQASTSQAGVLFLRIAAGARAAG
                     MGESFVALANDATATHWNPAGLGQYPLTATWLDWELPVAEPIEKMALTHNGVPEANYK
                     RFAVWAWASGQLYHWNGRQWQTSHVYRPAGDETLESIVRKYTKISDDSTVAALMEKVA
                     EANSRFTKAALRDWREKVLALLPQESADRTSLANAFDELATNWSHLTVDEGTAAQVGE
                     QVGAVTADGSLTPEEFDKLRATLAHTGRRFLLEEIRLPYNLAFTAPVTALTG"
BASE COUNT          351 a          495 c          507 g          316 t
ORIGIN      
        1 gtcaatttaa tctctcattt gtcgtgccga aggacatcag ttacggctcc aaaacagcca
       61 agatttcggt ctacatagcc ggcggatcaa ccgacggcgt aggcctcttg gacagtttaa
      121 ccctggccgg ggcgccggta gccatcaccg acagcctggg accggaaatt gacctgcgtt
      181 tcgacgggcg gcccgcggcc gacggcgatt tcgtccgggc cgacgcaaca ctcaccggca
      241 tgctccgtga tcagaatgga attaacatca ccggcgagcc cggacatgga attgtgctca
      301 ccctggatgg aaatcgccag gccagttacg acctgaccga ccgctttgca tacgatccgg
      361 gaagttttca agcggggacg gtcaccttca ccctgccgaa tcttgccagc ggagcgcaca
      421 cggttgaact caaagcctgg gattcggcca acaactcggc ggttcgcaga gtcaccatcc
      481 aggcggcgtc cgaggggtcg ctcgccatcc gggatttctt ggcctatccg aacccgttcc
      541 gcgaccagac caccttctgc taccagttga cccgcgatgt ggatcagatg aaaattatga
      601 ttttcaccga atcgggccgt caaatcaaaa ctattgaagg cccgaaccag ttggccggct
      661 accatgccga tctggtctgg gatggttgcg accagcgggg tgaccgggtg gccaacggcc
      721 tgtacctctg caaggcggtg gcgcagggcc gggtagcggc gggaacgaat gcggtcacga
      781 aaaccgcgga agaattcggt aaagtactcg tcgtacgtta gcaatcgatg cttatgatgc
      841 ggcggacaaa ggaggagcaa gtgaaacgcg cagtcggcat gctcaccgta gcaatgcttt
      901 tcgtcagttt ttcatatcgc cagggacagg cgtccaccag tcaggccgga gtgctcttct
      961 tgcgcattgc ggcgggcgcc cgggcagccg ggatggggga atcgttcgtg gctttggcca
     1021 acgatgccac tgccacccac tggaacccag ccggtctggg gcagtacccc ctgaccgcca
     1081 cctggctgga ctgggagttg ccggtggccg agcccattga aaaaatggcg ctcacccaca
     1141 acggagtccc ggaagccaac tacaagcggt ttgccgtctg ggcctgggct tccggccagc
     1201 tgtatcactg gaacggtcgc cagtggcaga ccagccacgt ctaccggccc gccggcgatg
     1261 aaaccctgga gagcatcgtc cggaagtaca ccaaaatcag cgatgattcg acggtggcgg
     1321 ccttgatgga aaaagtggcg gaggccaatt cgcgtttcac caaagcggcg ctccgggact
     1381 ggcgcgagaa ggtcctggcg cttctgccgc aggaatcggc cgatcggacg agtctggcca
     1441 atgcttttga cgaactggcc accaactggt ctcacctgac cgttgacgaa gggaccgcag
     1501 cccaggtcgg ggaacaggtt ggcgccgtga cggctgacgg atcactgact cccgaagaat
     1561 tcgataagct ccgggccact ctggcccata ccggtcggcg ttttcttctg gaagaaattc
     1621 gtttgcctta caatctggct tttacggcac cggtgacggc tttaaccgg
//
LOCUS       DBNGZD010000405         2117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1312738, whole genome shotgun sequence.
ACCESSION   DBNGZD010000405 DBNGZD010000000
VERSION     DBNGZD010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1312738"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="AC16GM_10495"
     CDS             <1..453
                     /locus_tag="AC16GM_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HIR2924136.1"
                     /translation="ALPSITGKIELVYEGEQEGTGAIAANLVGRAVKEVFLAYFPPPR
                     KPKQTVEEEDYTYSEVLSYFSNRHHIELSDESPFEEYSAALHKVKGLKKLAAAHFQTS
                     SPEEEALVMELVLEGLHQHNLIAKEHLDKTFRYSDVLATMLKDMEEAD"
     gene            455..1552
                     /locus_tag="AC16GM_10500"
     CDS             455..1552
                     /locus_tag="AC16GM_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR2924137.1"
                     /translation="MRVRYSQYDPEAVRKLKDFAELLKLFNYLVLASSGDIEKVLRYM
                     KMLQKQGYLDREFNLDDFVNQMGEEGYLQKVKNRLSLTPKGERKIRQDSFQAIFSNLK
                     KGGFGNHPIPHEGGGTREQLPERRPYEFGDETENIDFPSSIKNLIHRAGLSSEFVFEE
                     KDLEVFETESVSSCATVLLLDISHSMILYGEDRITPAKQVAMALTELIMTHYPKDSLN
                     VVLFGDNAKEVPVKELAYASVGPFHTNTKAGLILARQILGRKKHPNKQIIMITDGKPS
                     VIMRPNGTIYRNPVGLDPVIVNRTLDEAVICRKRRIPITNFMIASDPYLQSFVEKLTE
                     LNRGRAYFTTPDRLGEFILWDFVSNRRKKIR"
     gene            1680..>2117
                     /locus_tag="AC16GM_10505"
     CDS             1680..>2117
                     /locus_tag="AC16GM_10505"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR2924138.1"
                     /translation="MKKLGITAMAAVLLLAAVVAFAQMSGKGKAAGKNNWSIDAKIIE
                     ACSCKLMCPCYFNDSPDKHFCQFNNVYSVISGHYGTTNLAGVKFWVSGDLGENFGDGT
                     AEWMAVTFDPATTQEQMDAIPKILAEIYPIKAEILGVEKKPISW"
BASE COUNT          536 a          567 c          550 g          464 t
ORIGIN      
        1 gcccttcctt ccatcacggg aaaaatcgaa ctggtgtacg aaggggaaca ggagggtacc
       61 ggcgccattg ccgccaatct ggtcggccgg gcggttaaag aagtgtttct ggcctatttt
      121 cccccgcccc gaaagccgaa acagaccgtg gaagaggagg attacaccta ttcggaggtc
      181 ttgagctatt tttccaaccg acaccacatc gagctttccg atgagtcgcc gtttgaagaa
      241 tattccgccg ccctgcataa agtcaaggga ttaaaaaagc tcgcggccgc ccattttcaa
      301 acctcctcac ccgaggagga ggctttggtg atggagttgg ttttggaagg gcttcaccag
      361 cacaacctga ttgccaagga gcatctggac aaaaccttcc gctactccga cgttttggcc
      421 acgatgttga aggatatgga ggaagccgac tgacgtgcgc gtccgctatt cccaatatga
      481 cccggaggcc gtccgcaagc taaaggattt cgccgagctg ttaaagcttt tcaattacct
      541 cgttctggcc tcttccgggg acatcgaaaa ggtcctgcgg tatatgaaaa tgctccagaa
      601 acaggggtat ctggaccggg aattcaattt ggacgacttc gtcaaccaaa tgggggagga
      661 ggggtatctc caaaaggtaa aaaaccggct gtctttgacc cccaaggggg agcggaaaat
      721 ccggcaggat tcctttcagg ccatcttttc caacctgaaa aaaggggggt tcggcaatca
      781 cccaatcccc cacgaaggag ggggcacccg cgagcagctg cccgaacggc gcccttacga
      841 attcggcgac gagaccgaga atatcgattt tccttcttcc atcaaaaacc tgatccatcg
      901 tgccggctta agctctgaat tcgtttttga agaaaaagac ttggaggtct ttgaaaccga
      961 atcggtttcc tcctgcgcca cggttttgct tttggacatc agtcactcga tgattctcta
     1021 cggagaagac cgcatcactc cggccaagca ggtggcaatg gctttgaccg aactgattat
     1081 gacccattat cccaaagaca gcttgaacgt ggtgcttttc ggcgacaacg ccaaggaggt
     1141 gcccgtcaag gaactggcct atgcctcggt cggcccgttc cacaccaaca ccaaagccgg
     1201 gcttattctg gcccggcaga ttctggggcg caaaaagcat cccaacaagc agattatcat
     1261 gattaccgac ggaaagccct ccgtgattat gcgccccaac ggcactatct accgcaaccc
     1321 ggtggggttg gacccggtaa ttgtcaaccg gacgctggac gaagcggtta tctgccgcaa
     1381 aagaaggatt ccgataacaa atttcatgat tgcctccgat ccctacctgc agagctttgt
     1441 ggagaagctg accgaactca atcgcggccg ggcctatttc accacgccgg accggctggg
     1501 ggagtttatt ctctgggact tcgtctccaa ccgccgcaaa aaaatccgtt aggatcaatc
     1561 ctctttttgt ctggttcaaa ttttataaat ccctgacatt tcttacttga caggtcctcg
     1621 aacggttggt tattttcacc cgcaggtaga aaaaaaccaa ataaaccaaa ggaggaggaa
     1681 tgaagaaatt gggtattacc gccatggcag ccgtgctgct tctggcggcg gtggtcgcct
     1741 ttgcccaaat gagcggcaaa gggaaagcgg ccggcaaaaa taactggagc atagacgcca
     1801 aaatcattga ggcctgttcc tgcaagttga tgtgtccatg ctacttcaac gattcgccgg
     1861 acaagcattt ctgccagttc aacaacgtct acagtgttat ctcgggtcac tacggcacca
     1921 ctaatctggc cggggtgaaa ttctgggtct ccggcgacct gggtgaaaac ttcggcgacg
     1981 gcacggcgga atggatggcg gtcacttttg accccgccac cacccaggaa caaatggacg
     2041 ccattcccaa aatcctggcg gaaatctacc cgataaaagc cgaaattttg ggtgtggaga
     2101 aaaagccgat tagctgg
//
LOCUS       DBNGZD010000406         6574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1938088, whole genome shotgun sequence.
ACCESSION   DBNGZD010000406 DBNGZD010000000
VERSION     DBNGZD010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1938088"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="AC16GM_10510"
     CDS             <1..1713
                     /locus_tag="AC16GM_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924139.1"
                     /translation="QHGDLPFQGYNIYQTRSLIKPDWKLIAVQDVNDGLGLVFDDVLD
                     PVTNVVVNKPVAFGSDAGVSHFIVISQDKVLGGPLHNGTPYYFAVTAYSADPNQAPTF
                     RVLETALNPIVAIPEGPVAGSDLTPAKVDTAVIYSRINPALSPSTDVVTAEVFNPEEV
                     TGHTYEVRFHPNQIVSIDSSVVPPETTFQVIPFQNSNGKSVYVTELWDLWDLNTNTAV
                     LTNRWNKAGDNNYDVVGGILVKVQGAYKAELQDVFHVNTGAHRRAWSGVNAGLQFFFN
                     SADYANNFFCSKYDPAVSTNPFTTVQIIFYPNDTARWQYAYNYRRDVVGPNNYRNIGY
                     FRVPFQVWDVVNNLQLNAAFIENARPPVDGNLDSLWDPTDSTTGGREYVFVFRSTYSP
                     TPDTFYLNHNINSECDIFDMLYAWWPLKRSATDTVDNGDYLEFLFANPSTANDRYTIR
                     TTAPVLTNAGLAKGQLSRVKAVPNPYFNRSAYELTQFDRQIKFTNLPTTCTIKIYNLA
                     GELVRTLQKTDPSTSQLTWDILSEARLPIASGIYLWRLEAPGIGEAQGKMVIFTEQEQ
                     LNNF"
     gene            1734..2816
                     /locus_tag="AC16GM_10515"
     CDS             1734..2816
                     /locus_tag="AC16GM_10515"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2924140.1"
                     /translation="MKKLWMGIAGLTLLLGGVEPVRAGNSNRIGTAGAQELRIPVAAR
                     SVALGGAVVANATGAEALYWNPAGAATLENTEVLFTHLRYIGGMDLNYGAVMHNFGSA
                     GNLGLSAKVLSVGDIEVTNEANPDGTGEIISPTFTTFGAHFARAMTDRVSLGASISVV
                     DERVMREQATGLAFDFGFQYQSGWHGLKLGLVIKNLGSQMRFDGPDFDLNIVPPGADP
                     GSNNRTTRTQSAAFDLPSSMQLGFAYDWLNGGENRVTTMAVFQSNNFSEDEYRIGGEY
                     AYRENYFLRASFVTNAQEVRAWWGRHINKPQSESQQQEGTYLYGAAFGGGVQAKIGDV
                     KAVFDYTFARVQNLEDNHFFTVKFLF"
     gene            2939..3205
                     /gene="rpmE"
                     /locus_tag="AC16GM_10520"
     CDS             2939..3205
                     /gene="rpmE"
                     /locus_tag="AC16GM_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR2924141.1"
                     /translation="MKEGIHPKYYDCVVACACGNRFTTRSTLKEIHVEICSNCHPFFT
                     GKQKLVDTAGRVERYRRKYAPKMETPEPGKPKAARKTQTTTKAH"
     gene            3341..4291
                     /locus_tag="AC16GM_10525"
     CDS             3341..4291
                     /locus_tag="AC16GM_10525"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR2924142.1"
                     /translation="MPDKNIGGQAVIEGVMIRAPRRVATACRKPSGEIVLRSEDYLPL
                     VKRIPFLRVPILRGLVAFLEMLVIGLKSLNFSASVAAEAAQKAGRKNPGKKSAADSFL
                     LFSTTVLAFAIAIAVFFYLPLLAANLFGIRRAQWAFNLAAGGIRVSLFIGYVWFLSLF
                     RDFRRVFEYHGAEHMSIFAYENDEELSTANIRKYPPEHPRCGTSFLMIVALGAIIIYS
                     FSDTIFFWVAGRAPSVPQRFLVHFSLLPFVAGSAFELLKLAGKTRENRLTKILIAPGL
                     WLQKITTRMPHDDQIEVAVAALHSAVTAAPASPLPVSQKA"
     gene            4294..5388
                     /gene="prfA"
                     /locus_tag="AC16GM_10530"
     CDS             4294..5388
                     /gene="prfA"
                     /locus_tag="AC16GM_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR2924143.1"
                     /translation="MLEILEKAEKRYAELSEKLSDPKVLTNQNLYRQVAREHHELEEI
                     VRTGTEFRKVLTAIDDDERIKTESADRELVDLAKGELSELYARREALEANLKRLLIPR
                     DPNDKKSVIVEIRAGTGGEEAALFAAELYRMYTKYAERHAWKIDVMNTNPTGLDGLKE
                     VIFGVTGEGAYGKLKYESGVHRVQRVPVTEASGRIHTSAASVAVLPEAEDVEIDIKPD
                     ELQIDVFRSSGPGGQSVNTTDSAVRITHKPSGLVVSCQDERSQLKNKIKALKILRARL
                     FDKAQREQKEKIDSARRAMVGSGDRSEKIRTYNYPQNRVTDHRIGLTLHKLEDVLSGE
                     LDEFIEGLQTQEQAERLSEAGQIVNEKKAE"
     gene            5483..6130
                     /locus_tag="AC16GM_10535"
     CDS             5483..6130
                     /locus_tag="AC16GM_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HIR2924144.1"
                     /translation="MIRIVIAAIAARLLSKQWNDLQKKLFFQYTRLLNEQEYVPSVLL
                     QRLLISGEDHRFFKHPGFDVIAICRAVWRCVFWGVREGASTIEQQLVRVLTGHYELTV
                     GRKCKEILLATLVTRVVPKSDVPALYLQIAYFGWRMNGFRQACIRLNLRPEVISIQDA
                     ALMIARLKYPEPRIAPPQRLVQIFRRHDHLIRLHQRHLGEGRFVALQPRFCHAPI"
     gene            6117..>6574
                     /locus_tag="AC16GM_10540"
     CDS             6117..>6574
                     /locus_tag="AC16GM_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924145.1"
                     /translation="MRPFEHSESAVSLINPRPSADELVKVAKNAWSSELFAIVRQWLS
                     EGIPYAFAHRPFLYETTREFLASRIGIHPKQVTIIGSARLGYSLAPGLNFGKAFNEQS
                     DLDFSAISDDLFERCVNDFRQWRADYKGGTILPAGPRQEGFWKSNFLDVP"
BASE COUNT         1491 a         1817 c         1844 g         1422 t
ORIGIN      
        1 cagcacggcg acctgccgtt ccaggggtac aacatttacc agacccgctc gctcatcaaa
       61 ccagactgga aactcattgc cgtccaggat gtcaatgatg gactggggct ggttttcgac
      121 gatgtccttg acccggtcac caacgtggtg gtcaacaaac cggtcgcttt cggcagtgac
      181 gcaggggtga gtcatttcat tgttatttct caggacaagg tgttgggtgg gccgctccac
      241 aatgggacgc cctactactt tgcggtgacc gcttattccg ccgatccgaa ccaggcgccg
      301 accttccggg ttttggaaac cgcactcaat ccgattgtag ccattccgga agggccggtg
      361 gcggggagcg atctgacgcc ggcaaaagtc gatacggcag tgatctacag ccgaatcaac
      421 ccggcattgt ccccttccac tgacgtcgtg acggcggaag tgttcaatcc tgaggaagtg
      481 accggccaca cctacgaggt gcggttccac ccgaatcaga ttgtctccat cgactcgtcg
      541 gtcgttccac cggagacgac attccaagtg atccccttcc agaactccaa cgggaagtcc
      601 gtctacgtca ctgagctctg ggacctgtgg gatttgaaca ccaataccgc cgttttgacc
      661 aatcggtgga acaaggcggg ggacaacaac tatgatgtgg ttggtggcat cctggtaaaa
      721 gtgcagggtg cgtacaaggc cgaactccag gacgtatttc acgtaaacac cggggctcac
      781 cggcgggcgt ggtctggcgt gaacgcgggc ctccagttct tcttcaacag tgccgactac
      841 gcaaacaact tcttctgcag caaatacgac cccgcggtca gcaccaaccc attcaccacg
      901 gtacagataa tcttctaccc caacgacacc gcacggtggc agtatgcgta caactaccgg
      961 cgggacgttg tgggacctaa taactaccgg aacatcgggt actttcgggt cccctttcag
     1021 gtgtgggacg ttgtgaacaa tctccagttg aatgcggcgt tcatcgagaa cgcaagacct
     1081 ccggtggatg gcaacctcga ttcgttgtgg gatcccaccg actccacgac gggaggacgt
     1141 gaatatgtct tcgtcttccg gagcacctat tctcccacgc ccgatacgtt ctacctcaac
     1201 cataacatca acagcgagtg cgacattttt gacatgctct acgcctggtg gccactcaaa
     1261 cgttccgcca ctgataccgt tgataacggg gactacctgg aattcctctt cgcgaacccc
     1321 tcaaccgcca atgaccgcta caccatccgg acgacggcgc cggtactgac taatgccggt
     1381 ttggccaaag ggcaactgag ccgggtgaag gcggtcccga acccctactt caaccggtcg
     1441 gcctacgaac tcacgcagtt tgaccggcag attaaattca ccaacctgcc gaccacctgc
     1501 accatcaaga tttacaacct ggcgggagaa ctggtgcgca cattgcagaa aaccgacccc
     1561 agtaccagtc aattaacctg ggacattttg tcggaagccc ggctacccat cgcctcaggg
     1621 atttacctct ggcgcctgga ggcgccgggg attggtgagg cccagggcaa gatggtgatc
     1681 ttcaccgagc aggaacaact caacaacttc taagggagag gagagcgagc gcgatgaaaa
     1741 aactatggat gggaatcgcc ggactgaccc tccttttggg aggggttgag cccgtacggg
     1801 ccggaaacag caaccgaatt gggactgccg gagcgcagga gctgcgcatt ccggtggcgg
     1861 cccgctccgt ggccctggga ggcgcggtcg tggctaacgc taccggcgcc gaagcgcttt
     1921 actggaatcc ggcgggagcc gccactttgg aaaacacgga ggtgctcttc acccacctgc
     1981 gctacatcgg cggcatggat ctcaactacg gcgcggtgat gcacaacttc ggcagcgcgg
     2041 gcaacctggg gttgtcggcc aaagtgctct ccgttggcga catcgaagtg accaatgagg
     2101 ccaacccgga cgggacgggc gaaattatct cgcccacctt taccaccttt ggcgctcact
     2161 tcgcccgggc gatgaccgat cgggtctcgc tgggagcgtc catttcggtc gtggacgagc
     2221 gggttatgcg ggagcaggcc accggtttag ctttcgattt tggcttccag taccagtccg
     2281 gctggcacgg tctcaaactg ggactggtaa tcaagaacct gggctcacaa atgcgctttg
     2341 acggtccgga ttttgatctc aacatcgttc ctcccggagc tgaccccggt tcgaacaacc
     2401 ggaccacccg gacgcagtcg gcggccttcg acttgccgtc ttcgatgcag ttgggcttcg
     2461 cctacgactg gctaaacggg ggtgagaatc gcgtcacgac gatggcggtg ttccagtcga
     2521 acaacttctc cgaagacgaa taccgcatcg gcggggagta cgcctaccgc gaaaactact
     2581 tcctgcgggc cagttttgtg accaacgccc aggaggtgcg ggcctggtgg ggccggcaca
     2641 ttaacaagcc gcagagcgaa tcacagcagc aggaggggac gtatctctac ggggcggcct
     2701 tcggcggcgg tgtccaggcc aagataggtg acgttaaggc cgtcttcgac tataccttcg
     2761 cccgggtgca gaacttggaa gacaaccact tctttacggt gaaattcctc ttctaaggca
     2821 tccgcctcag gcggatcaat tgagaaaaat ccccccgcca ctggcggggg gatttttctt
     2881 tccggctcaa ttgctttttg gcgttttgcg tatagattag gttgtaagga ggacagcttt
     2941 gaaagaaggc atacacccca agtattacga ctgcgtggta gcctgcgcct gcgggaaccg
     3001 gtttaccacc cgctcgacgt tgaaggaaat tcacgtcgaa atctgctcca attgccaccc
     3061 gttcttcacg ggcaagcaga aattagtgga caccgccggc cgggtggagc gctaccgccg
     3121 gaagtacgcg cctaaaatgg aaacgccgga gccgggcaaa cccaaagcgg cgcggaaaac
     3181 tcagaccaca accaaagcgc attagcgtaa cgaagcgggc ggggttcggt gcgttaccgt
     3241 tcgtccccgc cctttttctt tccgctgttg tcgggaagaa ggccactgcc cacacgcagt
     3301 ccgggtgggg tgagcgctac gaaagatcgg gaatcaggca atgccagata aaaacatcgg
     3361 cggtcaggcc gtcatcgaag gggtaatgat tcgggcgcct cgacgggtgg ccactgcctg
     3421 ccggaagccc tccggcgaaa tcgtcctgcg ttccgaagac taccttccgc tggtgaagcg
     3481 catccccttc ttgcgggtgc cgattctccg cggtttagtt gcctttctgg agatgctggt
     3541 gattggcctc aagagtctga acttttcggc ctccgtcgcc gccgaagcgg cccagaaggc
     3601 gggaaggaaa aatcccggga agaagtcggc ggcggactcc tttctgctct tcagcactac
     3661 cgttctcgcg tttgccatcg ccattgcggt gttcttctac ctgccgcttc tggcggccaa
     3721 tttattcggc atccggcgcg cgcagtgggc cttcaactta gcggcgggcg gcatccgggt
     3781 atccctcttc atcggctacg tctggttcct ctcgcttttc cgcgacttcc ggcgggtctt
     3841 tgaataccac ggcgccgagc acatgtcgat tttcgcgtat gagaacgacg aggagctctc
     3901 caccgccaac attcgcaagt atccgccgga acacccgcgc tgcggcacca gttttctgat
     3961 gatcgttgct ctgggagcga tcatcatcta ctcgttctcg gataccatct tcttttgggt
     4021 agccggacgg gcgccctcgg taccgcaacg gtttttggtc cacttctctc tgttgccctt
     4081 cgtggcgggg agtgcctttg agttactgaa gctggccggg aaaacgcggg agaatcgcct
     4141 gaccaagatt ttaattgcgc ccggtctgtg gctgcagaaa atcaccaccc ggatgcccca
     4201 cgatgaccag attgaggtag cggtggcggc gttacacagc gcggtgacgg ccgctcccgc
     4261 ttccccactt ccggtgagtc aaaaggcata accatgctgg agattctcga aaaagccgaa
     4321 aagcgctatg ccgaactttc cgaaaagctt tccgacccga aagttctgac caaccagaac
     4381 ctctaccggc aggtggcccg ggagcaccac gaattggagg aaatcgtccg tacaggtacc
     4441 gaattccgca aggtgttgac cgccattgac gatgatgagc ggatcaaaac cgagtcggcg
     4501 gatcgggagt tggtcgatct ggcgaaggga gaattgagcg aactctacgc ccggcgcgag
     4561 gctctggaag cgaatctcaa acggctcttg atcccccgcg atcccaatga caaaaaaagc
     4621 gtcatcgtcg aaatccgggc cggcaccggc ggcgaagaag cggcgctctt tgccgccgaa
     4681 ctgtaccgca tgtacaccaa gtatgccgaa agacacgcct ggaagatcga cgtgatgaac
     4741 actaacccaa ccggtctgga tggtttgaaa gaagtcatct tcggggtcac cggtgagggg
     4801 gcctacggaa aattgaagta cgaatcgggc gtccaccgcg tgcagcgtgt accggtgacc
     4861 gaggcctccg gccgtattca cacgtcggct gcgtcggtgg ccgttctgcc cgaggccgaa
     4921 gacgtagaga tcgatattaa accggatgaa ctccagatcg acgtcttccg ctcctcgggg
     4981 ccgggagggc aatcggtgaa taccaccgat tcggcggtac ggatcacaca caaaccatcc
     5041 ggcctcgtcg tttcgtgtca ggacgagcgg tcgcagttga agaacaaaat caaggcgctc
     5101 aaaatcctcc gggcgcggct cttcgacaag gcgcagcgcg agcagaagga aaaaatcgac
     5161 tcggcccggc gggcgatggt cggctccggc gatcgctcgg agaaaatccg cacctataac
     5221 tatccgcaga accgggtgac cgaccaccgc atcggcctca ccttgcacaa attggaagac
     5281 gtgctctccg gcgaactgga tgagttcatc gaaggcctcc agacgcagga gcaggcggag
     5341 cggctttcag aagctgggca gattgtcaat gaaaaaaaag ctgaatgatg ttgttcttga
     5401 cagttgcgta gggtactcat ttaatcccta ctgacagatc tttaacggta aagaccgcag
     5461 ttttggaatt aaagggagaa gattgataag gattgtaata gcagccatcg cggcaagact
     5521 tctcagtaaa caatggaatg atttgcagaa gaaactattt ttccaataca cacgattgtt
     5581 aaatgagcaa gagtatgttc cctcagtatt gctgcagcgt cttcttattt caggagagga
     5641 tcaccgcttt ttcaaacacc cagggtttga tgtaattgcc atttgccgcg ctgtttggag
     5701 gtgtgttttt tggggtgtcc gagaaggggc aagtacgatt gaacagcaac tggtacgcgt
     5761 ccttacagga cactacgagt taaccgtcgg gcgaaagtgt aaagaaattc ttttggcaac
     5821 tttggtcacg cgtgttgttc cgaaatccga tgttcccgca ctttatttac agattgccta
     5881 ttttggttgg cgaatgaacg ggtttcgtca agcttgcata aggttaaatc ttcgaccaga
     5941 agtaatatca attcaggatg cggctttgat gatagcgaga ttgaaatatc cggagccacg
     6001 cattgcacca cctcagcgcc ttgttcagat tttcagacgc cacgaccatt tgattaggct
     6061 ccatcaaagg catctcggtg aaggtcgttt cgtggctctg caaccacgtt tttgtcatgc
     6121 gcccatttga acattcagaa tcagcggtct ctttaattaa tccccgtcct tcagccgatg
     6181 agttagtcaa ggttgcaaaa aacgcgtggt cttcggagtt atttgctatc gttaggcaat
     6241 ggctgtcgga gggaattcca tatgctttcg cacaccggcc ttttctttat gaaacaacaa
     6301 gagaatttct agcttcgaga ataggaatac atcctaaaca agttaccatc attggaagtg
     6361 cgcgtcttgg gtattcactt gctccaggat taaattttgg gaaagcgttt aatgaacagt
     6421 cagacttgga ttttagcgcg atctctgatg atttgtttga acggtgtgta aacgacttta
     6481 gacaatggag agcggattac aagggtggta cgattttgcc cgctggacca cgacaggaag
     6541 gcttttggaa aagcaatttt ttggatgtgc caca
//
LOCUS       DBNGZD010000407         5155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_758482, whole genome shotgun sequence.
ACCESSION   DBNGZD010000407 DBNGZD010000000
VERSION     DBNGZD010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_758482"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="AC16GM_10545"
     CDS             <1..1140
                     /locus_tag="AC16GM_10545"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR2924146.1"
                     /translation="AATRPDRMNLANIRKGEYEGLKEEIGKNPDRKPDFGPERLHPRA
                     GATVIGARMPLIAYNIYLGTNDVSIAKNIAKKVRESSGGLPAVKALGFAIKERNLVQV
                     SMNLTNYLKTPIHEAVEAVKAEAGRLGVPVVSTELVGLAPLDSFLASAEYYLKLEHFK
                     RDQILETKLLSLFSERRESLADFVDRVASAEPTPGGGSVAALCAALAAALTAMVCRLT
                     LGKKKFAAVETEIKEVLQKAEQLRQVTFQLIQKDTAAFQQVMEGYKLPKETEKEKESR
                     DRAIEKATLQASEIPLEVMRRGLEVLRLSDVVAEKGNPNSRSDAGVAALLARAATEGA
                     WLNVEINLQSIRDAEFVKRVTEEGQKILSQARLPRGNVQTVLAAN"
     gene            1170..3002
                     /locus_tag="AC16GM_10550"
     CDS             1170..3002
                     /locus_tag="AC16GM_10550"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6640 family putative metalloprotease"
                     /protein_id="HIR2924147.1"
                     /translation="MRCFGVTILLFFLSNVPQSQAKLSQAYEAGLIERVKSLRPAQSG
                     VVLAGAEGLPRPLCGTPIALEANYNYENLSSETQSVLAQYLARPTTGVTQITPLGFIR
                     VHYDTASGDVDRVPSADVLPLNGIPDFIDDAMTVLDSVWLREIVDLGYRKPLVDGTAG
                     GDSLLDVYFQQLGPSYLGQTFVDVGNLGPQRNQGTAYVELDNDYVFPQYPTPAARQRI
                     LRVTAAHEFFHAVQFAYDIREFEFTDPGNSSTFRPYWYEMSSTWMEDIVYDTIDDYHS
                     LVKYVLRYPWLSFRTFSYNPSDTAGVFHPYAQALWNHYLVARFADTTLIRTIWTLCGA
                     VNGFNFFPAVNQALSAKMSSFLASFREYCIWNYYTGSREVPGKYYRLEGESFPVGAFV
                     DPQPQQSVIVGDAQPPVQDLGCTYYFIVPNASQPDSFQIFFTVLSGAVAQWDIAGFGR
                     DTTGDTVFISFMPVGAASWLARYPAWKTFTDQVVLPIALQEPIDATIGNYGYHYVFGF
                     QGPPPASDQFSDPYPNPIFLPQQTTVRFKVNLARDAQVEFSVHDLSGQVIHEERPKKF
                     LAGVESFLTWDGLNDEQQMVASGVYLCAIRVGDKTKVFKLAVVR"
     gene            2999..3523
                     /locus_tag="AC16GM_10555"
     CDS             2999..3523
                     /locus_tag="AC16GM_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924148.1"
                     /translation="MNPRLVRLAKLAALVALAYVLKLPFLSIPNVEFFTYLCFAAGYL
                     FGIWSGALTAAAAMTLYSLFNPYGPAGPILTAAKVLAMTFVGIAGGLTFHLRFVPRAT
                     ILSLAAAALLGLVLTLQFDLITNLAIVVTTGQFWPVMAAAVPFAAIHIGSNTVLFVLF
                     NPLLFRLAKLEHRL"
     gene            3643..>5155
                     /locus_tag="AC16GM_10560"
     CDS             3643..>5155
                     /locus_tag="AC16GM_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2924149.1"
                     /translation="MATTVSDSLVRDSLGAQVRDSLVKENAVRAAQELGAAEITAIVP
                     QDFGDLLRRFGQFDAAGSGPFGQPKQLILWGVPVREAGEIWNGARLFSALQFPQTGTV
                     DLHALPLEEIESLSVGESGNETKSETVETKGVATPENAPYARARLKRGPKGLSVLSVV
                     FTRQLGRAGNFSFSGGFKKARGKDDPRSYDGKSFNGHYRNRFRNGWSLAGDYHLYDEK
                     RGVVAWEELAFLDLRRKAKAEHLSLTLIRKWHERRRLATELFYSRDRVTLKDNAGRFA
                     NGYQQKRPGGTLSWQENLSGSQQITGTSRFFYDFFEQDKTQRLYHVVLKAQDAWKPNN
                     LITLVLSGSAEKQRFQPLQFYGGISMALAPAKRMPEFKVSVGQKGDFPTAFDRFARPD
                     SVAGLNYRESGNANLAVGRTKEIEGSVAWRKSAFAVGTTGTYQWLDKKILWQNRDPNL
                     QYGDWRPNNEDVKTWGFSTFISGTLPRWGSAYLVYGYKNPVIVQTEASLPLAPK"
BASE COUNT         1116 a         1401 c         1458 g         1180 t
ORIGIN      
        1 gcggccacgc ggcccgatcg gatgaatttg gccaatatcc gcaagggaga gtacgaaggc
       61 ctgaaggaag aaatcggcaa gaatcccgat cgcaagcccg attttggccc ggaacggctg
      121 catccgagag ccggggcaac agtcatcgga gcgcggatgc ctttaatcgc ctataacatc
      181 tacctcggga cgaatgacgt atccattgcc aaaaacatcg ccaaaaaagt acgcgaatct
      241 tcgggcggtc tgcccgcggt caaagcgctg gggtttgcaa tcaaggagcg gaacctcgtc
      301 caggtctcaa tgaacctgac gaactacctg aaaactccga ttcacgaggc agtcgaagcg
      361 gtcaaagccg aagccggacg tttgggcgtg ccggtggtct caaccgaact ggtaggtctc
      421 gcgcccctgg attccttttt ggcttcggct gaatactatc tcaaacttga gcatttcaag
      481 cgcgaccaga ttctggaaac gaagctcttg agtctgttct ccgaacgacg agaatctctg
      541 gccgatttcg ttgaccgcgt ggcttccgcc gagcccacgc ccggcggcgg gagcgtggcg
      601 gccctctgcg ccgctctggc tgccgccttg acggctatgg tctgccgatt gaccctcggt
      661 aagaagaaat ttgccgcggt ggagactgaa atcaaagaag ttctgcaaaa agcggagcaa
      721 ctccgacagg tgacgttcca actgattcaa aaggacacag cggcctttca gcaggttatg
      781 gaagggtaca aactgcccaa agaaaccgaa aaagagaaag agagcagaga ccgggcaatt
      841 gaaaaagcta cgctgcaggc ctcggaaatc cccctggagg tcatgcggcg ggggctggaa
      901 gtgttgcgtt tgagtgatgt tgtggcagag aagggaaatc cgaactcccg gagcgatgcc
      961 ggcgtggcgg cgcttttggc ccgggcggcc acagaaggtg cgtggctgaa tgtggagatt
     1021 aacctgcaat cgattcgtga tgccgaattc gtaaagcggg tgacagagga aggccagaag
     1081 attctgagcc aggcccggct gccgcgcggc aatgttcaga ccgttctggc ggcgaactga
     1141 tgaaaaagac aaagcggaaa ggacctgcga tgcgctgttt tggggtgaca attcttcttt
     1201 ttttcttgag taacgtcccg caaagccagg cgaaattgag ccaggcctac gaagcgggat
     1261 taattgagcg cgtaaaaagt ctgcggccgg ctcaaagcgg ggttgttctc gctggcgctg
     1321 agggtttgcc ccggccgttg tgcggtaccc cgattgcgct cgaagcgaat tataattacg
     1381 aaaatctctc ttctgagact caatccgtct tggcgcaata tctggcgcga ccaaccacgg
     1441 gggtcactca aatcacaccc ctgggattta tacgggtcca ctatgatact gcttctggcg
     1501 atgtggaccg cgtaccctca gccgacgtgc tgccgctaaa cggcattccc gatttcatcg
     1561 acgacgcgat gacggtgctt gattcggtct ggctgcgcga aatagttgat ttagggtatc
     1621 ggaaaccact ggtggatggt accgccggcg gagatagttt gctcgacgtc tactttcagc
     1681 aactggggcc cagctaccta gggcagacgt tcgtcgatgt cggcaacctc ggtccgcagc
     1741 gcaatcaggg aaccgcttac gtggagctgg ataacgacta cgtgtttccg cagtatccga
     1801 cgccggcggc gcgtcagcgc atcctgcggg tgacagcggc ccacgagttc ttccatgcgg
     1861 tgcaatttgc ctacgacatc cgcgagtttg agttcacaga tccgggcaac tcaagcacct
     1921 tccggccgta ctggtacgag atgagttcca cttggatgga agacatcgta tacgatacca
     1981 ttgacgacta ccactctctg gttaagtacg ttcttcgata cccctggctc tcgttccgca
     2041 ctttctctta caacccaagc gacacggcgg gcgtcttcca tccctatgcc caagcactct
     2101 ggaaccacta tctggtggca cgcttcgccg atactaccct catccgcacc atctggaccc
     2161 tctgcggtgc ggtcaacgga tttaatttct ttccagcggt gaaccaggcg ctctcggcta
     2221 aaatgtcgag ctttttggct tcgttccggg aatactgtat ctggaactac tataccgggt
     2281 cccgtgaagt accggggaag tactaccggc tggaaggaga gtcgtttccc gtcggcgcct
     2341 tcgttgaccc ccagccgcaa cagtcggtga tagtcggtga cgcccagccg cctgtccaag
     2401 atctggggtg cacgtactat ttcatcgttc ccaatgcaag tcagcccgat tcgttccaaa
     2461 ttttctttac agtgctaagc ggagccgtgg cacagtggga catcgccggc ttcgggcgcg
     2521 acaccacggg cgataccgtc tttatctctt ttatgccagt cggcgctgct tcgtggctgg
     2581 cacgctaccc ggcctggaag acttttacgg atcaggtcgt actgccgatt gccctccagg
     2641 aaccgattga tgccacgatt ggcaactatg gctatcatta cgtctttggt ttccagggtc
     2701 cgccaccggc cagcgaccag tttagtgatc cgtacccgaa ccccatcttc cttcctcaac
     2761 aaacaacggt ccgatttaag gtaaatctgg ctcgggatgc tcaggtagaa ttctctgtac
     2821 acgatctgtc gggtcaggtt attcacgaag aacgaccgaa gaaatttctg gcgggtgtcg
     2881 agtccttcct aacctgggat ggcttgaatg atgagcaaca aatggtggcc agcggcgttt
     2941 acctctgcgc aatcagggtg ggagacaaga ccaaagtgtt taagctcgcc gttgtgcgat
     3001 gaatccacgc ctcgtccgcc tggccaagct ggcggctttg gtggctttag cgtatgtgct
     3061 caaacttcca ttcctttcga ttcccaatgt ggaatttttc acttacctgt gctttgcggc
     3121 cggatatctc ttcggcatct ggtcgggagc gctgacagcc gcggcagcga tgaccctcta
     3181 ttcacttttc aacccctacg gcccggccgg accgatcttg acagcggcaa aagttctggc
     3241 aatgaccttc gttggaatag cgggaggttt gacgtttcat ttgaggttcg ttcctcgggc
     3301 aactattcta agtttagcgg ctgccgccct tttggggttg gttctgaccc ttcaatttga
     3361 tctcatcacc aatctggcca tcgtcgtgac gaccggccag ttctggccgg tgatggcggc
     3421 ggcggtgcct tttgccgcca tacacatcgg ttcgaataca gttctctttg tactgttcaa
     3481 tccgcttctt ttccgtttgg cgaagctgga acaccgcttg tgagttactt tggttcacac
     3541 attcgttcca cactccggtt attttctttg cttcttttga tttcaacact ccattctctt
     3601 ttcatctggt ttaatacggc ggctctccac gctcaggaga gcgtggctac aactgtctcc
     3661 gactcgttag ttcgggattc tctgggagca caggtgcggg actctctcgt gaaagagaat
     3721 gcggtccggg ccgctcagga actgggcgcc gccgaaatca ccgctatcgt gccgcaggat
     3781 tttggggacc tcttgcggcg cttcggtcag tttgacgctg cgggtagcgg gccgttcggt
     3841 caacccaagc aactcatcct ctggggtgtt cccgtgagag aagccgggga gatctggaac
     3901 ggcgcgcgcc ttttttcggc gctccaattt ccccagaccg gcacggttga cctccacgcg
     3961 ctgcctttgg aagaaattga gagtttaagc gttggtgaat cagggaatga gacaaaaagt
     4021 gagacggttg aaaccaaagg agtcgctacc ccagagaatg cgccgtacgc ccgggcacgg
     4081 ttgaagcggg gaccgaaggg actgagtgtt ttaagcgtcg ttttcacccg ccaactgggc
     4141 agggccggaa atttttcctt tagcggtgga ttcaaaaagg cgcggggcaa agatgatccc
     4201 cgttcctatg acgggaaaag tttcaacggg cactaccgga atcgcttccg gaacgggtgg
     4261 tctttggccg gtgattatca tctctatgat gaaaagcggg gcgtcgtcgc ctgggaggaa
     4321 ctggccttcc tcgacctgcg ccgcaaagcc aaagccgagc acctatcgct gacgcttatc
     4381 cgtaagtggc acgagcgccg taggcttgcc acggaacttt tttacagtcg tgaccgggtg
     4441 actctgaagg ataatgcggg gcgctttgcc aacggttacc aacagaagcg gccgggtggc
     4501 actctgtcgt ggcaggaaaa cctgtcgggg tctcagcaga taaccggaac gagccgtttt
     4561 ttctatgatt tctttgagca agataaaact cagcgtctct accacgtcgt tttgaaggcg
     4621 caagatgcct ggaaaccaaa caatcttatt acccttgttc tctccggttc cgccgagaaa
     4681 cagcgtttcc agccgctgca gttctatggg ggaatcagta tggcattggc tccagcgaag
     4741 cgaatgcctg aattcaaagt ttccgtcgga cagaagggcg attttccgac ggccttcgac
     4801 cgttttgcac gacccgactc ggtggcgggc ctcaactacc gggagtcggg caatgccaac
     4861 ctggccgtgg ggcgaacgaa ggaaattgag ggatccgttg catggaggaa atctgcattt
     4921 gcggtcggaa ccaccgggac ttaccagtgg ttagacaaaa aaatcctctg gcaaaacaga
     4981 gatccgaacc tgcaatacgg agactggcga cccaataacg aggatgtgaa aacctggggc
     5041 ttctcgacct ttattagcgg aacgctgccc cgctggggaa gcgcctactt agtctacggg
     5101 tacaagaatc cggttattgt tcaaaccgaa gcgtcattac ccttggctcc gaagc
//
LOCUS       DBNGZD010000408         1810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_827557, whole genome shotgun sequence.
ACCESSION   DBNGZD010000408 DBNGZD010000000
VERSION     DBNGZD010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_827557"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16GM_10565"
     CDS             <1..195
                     /locus_tag="AC16GM_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR2924150.1"
                     /translation="MRMYDLKRCSVPLTRETLRKYDCVVIATDHSCYDYEFITQNASL
                     VVDTRNATNGKARNGNVVKA"
     gene            complement(924..1403)
                     /locus_tag="AC16GM_10570"
     CDS             complement(924..1403)
                     /locus_tag="AC16GM_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR2924151.1"
                     /translation="MSTIFHIFLPEKRVFIRRNHPGRHLWRSQPRRSAIALVLILLIT
                     ALLSFSGLGCGGSVGTEAVAPISSGSGSGTGVATLAWNAPTTNADGSTLTDLAGYKIY
                     FGVTPGVYTKSIDVGSNTTHQVSNLTVGTTYYFTVTAYNTAGIESNYATPPVSKTIN"
BASE COUNT          465 a          440 c          538 g          367 t
ORIGIN      
        1 atgcgcatgt acgacctcaa gaggtgctcg gtccccctga ccagggaaac actccggaaa
       61 tacgactgcg tcgtcatcgc caccgaccac tcgtgctatg attacgagtt catcacccag
      121 aacgcctccc tggtcgtgga caccaggaac gcgacgaacg ggaaggcacg aaacggaaac
      181 gtcgtgaagg cctagcgagg cattgtctca gaccaaggaa acaaaaccag gacctttgaa
      241 tcaaaacaaa agcgtctcgc gcaaagcccg caaagggcgc aaagttttag gtcttctcct
      301 tactgggtgg gtaatcgggc gtcaccggaa ttacacttgg ttttttttgg gcggctcatc
      361 gcttagccgc ctctgtcatg cccgttagag caattgtctt ttaacgttta tcttgcattc
      421 tgaagaagca ttatggcatt cggcgttttg agggggcatg ctcaacggcg gctttcgcgt
      481 cagagccgcc cggcgcaggg gaaaggcaaa agcataggtc tttacccatc cccgcacgtc
      541 cccggagcag ccctacgaaa tttttgggca cccgccttgg cctaaggaag cgttcccctg
      601 ggggcgaccg cgcattgtct gaggccgaag gccgagtttg cgcgggcgag cggacgtggc
      661 cttaggcggg tcaaaaattt cgttcaggac gcgaagggga tgtgcaaaaa aatcaaagaa
      721 caaacagcag ataaagcttc cagagcgcgc cgtctaacaa ttacattgtt ttttacccac
      781 tcgatatgga gatgactctt taaagataat gcgaagaaaa ggtttcaaga ggccaggcgg
      841 agccaggaaa gagtgggccc ctctgcgcga tcatagagga gcgcacagag gggaaggagg
      901 gagcccgggg agggtgctta tcatcaatta atggttttac ttactggcgg cgttgcataa
      961 ttgctctcaa tgccggcggt gttgtaggcc gtaaccgtga aatagtaggt cgttcctacg
     1021 gtcaggttgc tgacctggtg ggtagtgttg ctgccgacat cgatgctttt ggtgtagacg
     1081 cccggagtaa caccgaaata tatcttgtaa ccggccaggt cggtgagcgt ggacccgtct
     1141 gcgttggtcg tcggcgcgtt ccaggcgagg gtcgctaccc cggtaccaga accggaccct
     1201 gacgagatcg gggcaacggc ttccgtaccc accgagccgc cgcagcccaa tcccgagaag
     1261 gagagtaacg cggtgatcag tagaatgaga acgagagcga tggcgcttcg ccgaggttgg
     1321 gaccgccaaa gatgacgtcc ggggtggttc ctgcggataa aaactctttt ttcagggaga
     1381 aagatatgaa atattgtgct catggcagta ttcctcccac acagatacaa aacgttaaag
     1441 ttgtaccata gtagaggaaa aaagggaaga aggatgtgat tagaatcaca ctctagaaag
     1501 atggatttta tcatgctata gttaaaattg gcttgattcc tccccaaaaa gtcgaaaaga
     1561 gcgtgtgaac tgcaggttat tgcgaaaaaa gtattaggaa ccaggcggaa ggagcgacga
     1621 atcccggtgg gacgagtccg atataaaggg gacggcgcca gggagaggcg ggatagtgcc
     1681 tccaagggcc gaaagcccgc aaaaaaagtg ctgacggctc ctgaggtggc tgatctcggc
     1741 tccggtgcaa agcaacgcaa aatggtcaag gggccggata ttcggcatgt ctaccgcgca
     1801 ggaggtgccc
//
LOCUS       DBNGZD010000409         3719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1453671, whole genome shotgun sequence.
ACCESSION   DBNGZD010000409 DBNGZD010000000
VERSION     DBNGZD010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1453671"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /gene="dnaG"
                     /locus_tag="AC16GM_10575"
     CDS             <1..1611
                     /gene="dnaG"
                     /locus_tag="AC16GM_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR2924152.1"
                     /translation="GCGKGGNVFTFLTEHEKMTFGEALRYLARRANIELPRTKSPQGQ
                     KESEDLYRATQVALAYFRESFADPRLGKEGKAYLRERELSAETIEEFQVGYAPFAWDG
                     LIAFAAERGVPAEMLFKVGLVARKDATAYSGGSRYYDLFRRRVIFPIFNLSNAPIAFG
                     GRVINTEDQPKYLNSPEHPLYQKGKTLFGLNLSKEEIRSTGKAVVVEGYLDLISVYQV
                     GIKNVVAPLGTAFTPDQARLLARFASEAILFFDPDAAGQSAVLRSIEHLIDQGFSVRV
                     ALLPAGEDPDTFARKHGQSWIQEVLQEAVGFLEYKKSRLEADFTTLSFSRKEEVLQDL
                     ALLASRVADPARRNLFLSEAGDTFGVELAVLLGYVKKLRRQSARPPVSRPEAEKAPRA
                     APHPGDSRERELLGLVFASADVLKDAAGFMHRSDWQNPALADLFELAVKHFHQEGKVS
                     LSGLLDSAGVEEKRQVLVKLSDWEAPDAELSWQMWQDHRKLVLKRRREEQIRLLSGQI
                     EVAQKQGHEEEAVRLTRKLREVSQTSAQ"
     gene            1691..1763
                     /locus_tag="AC16GM_10580"
     tRNA            1691..1763
                     /locus_tag="AC16GM_10580"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Ile,seq:cat)
     gene            1906..1982
                     /locus_tag="AC16GM_10585"
     tRNA            1906..1982
                     /locus_tag="AC16GM_10585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1940..1942,aa:Val,seq:cac)
     gene            2204..2941
                     /locus_tag="AC16GM_10590"
     CDS             2204..2941
                     /locus_tag="AC16GM_10590"
                     /inference="COORDINATES: protein motif:HMM:NF049746.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR2924153.1"
                     /translation="MATAGGMISVQKDLQMLLTLQNVDYDLLELERSKDYLPDMIRTL
                     EQQTTEAQENLKSLEKELLEQNLEQKRLELETATARDELSRYQKQMRDIKTNKEYDAL
                     MKEIDKRKGTIAADEDLLLTVMGTLEELTEKIEEAKKRQAEVKKTNEEQLVNLKQQLS
                     SIGQKIKMKEDERKNITVRIDRSTFSVYERVRKGKGGHAVVAVRKRACAGCYKSLPPQ
                     RVAEIRRGDTLITCDNCGRLLIWTGED"
     gene            3041..>3719
                     /gene="guaB"
                     /locus_tag="AC16GM_10595"
     CDS             3041..>3719
                     /gene="guaB"
                     /locus_tag="AC16GM_10595"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR2924154.1"
                     /translation="MATLQPKKIEKVGLTFDDVLLVPAKSEILPRDVDVSVIIARDIR
                     LNIPILSAAMDTVTESRLAIALAREGGLGVIHKNMSIEAQAAEVDRVKRSEAGMIVHP
                     ITLAPERPIGEALEVMRRYSISGIPVTKRGRLVGILTNRDLRFEKNLSRKIGEVMTKK
                     NLVTAPEGTELEAAKAILQKHRIEKLPIVDRRKTLKGMITVKDIMKQIQHPHACKDKR
                     GRLRVGGA"
BASE COUNT          890 a          989 c         1102 g          738 t
ORIGIN      
        1 ggctgcggca agggggggaa cgtcttcacc ttcctgacgg agcacgaaaa gatgaccttc
       61 ggcgaggcgc tccggtattt ggctcgccgg gctaatatcg aactgcccag gaccaaatca
      121 ccgcaagggc agaaggaaag tgaagacctc taccgcgcca cgcaggtggc tctggcttac
      181 ttccgggaaa gttttgcaga tcctcgtttg ggcaaagaag gaaaagccta tctccgtgag
      241 cgtgaactat ccgccgaaac tatcgaagaa tttcaagtgg gatatgcgcc ctttgcgtgg
      301 gatggtctga tcgcctttgc cgcggaacgt ggtgtgcccg ccgaaatgct tttcaaagtc
      361 ggcctggttg ccagaaagga cgcaacggcc tattccggcg gcagccggta ctacgacctc
      421 ttccggcggc gggtgatctt tccgattttc aacctttcga acgcgccgat cgctttcggc
      481 ggccgggtga tcaataccga agatcaaccg aaatacctta actctccgga acacccactc
      541 taccagaagg ggaagacact tttcggattg aatctctcaa aagaggaaat tcgttccacc
      601 ggaaaggcgg tggtggtcga gggttacctt gatttaatct cggtgtatca agtcgggatt
      661 aagaatgtgg tggcgccttt gggaacggcg ttcactcccg atcaggcgcg ccttttggcc
      721 cgctttgcgt cggaagccat tctctttttc gatcccgacg ccgccgggca gtcggccgtc
      781 ctgcgctcga ttgagcattt gattgatcag gggttttcgg tgcgggtggc tctgttgccg
      841 gccggtgaag acccggatac gtttgcacgc aagcacgggc agtcatggat tcaggaggtc
      901 ctgcaggagg ccgtcggttt tctggaatac aagaagagcc gcctggaggc cgatttcacg
      961 acgctctctt tttcacgaaa agaagaggtg ctccaggact tggccctctt agccagccgg
     1021 gtggccgatc cggcccgccg taatctcttc ctctccgaag ccggtgatac cttcggagtg
     1081 gagttggctg tcctcttggg ctacgttaaa aagttgcggc ggcagtctgc ccggccgcca
     1141 gtcagtcgcc ccgaggccga aaaagcgcca agagccgccc cgcacccggg tgacagccgg
     1201 gagcgggaac tgctgggtct ggtctttgct tccgcggacg tgctcaagga tgccgccggg
     1261 tttatgcacc gcagcgactg gcagaacccg gctctggctg acctgtttga gctggcggtg
     1321 aagcactttc accaggaggg gaaggtctcg ctatccggct tgctcgattc tgccggagtt
     1381 gaagaaaagc gacaggtgtt agtgaaattg agtgactggg aggctccgga tgcagaactc
     1441 tcctggcaaa tgtggcagga tcaccggaaa ttggttttga agcgccggcg ggaagagcag
     1501 atacggttgt tgagtggaca gatagaagtt gcccaaaaac agggacatga agaggaagcg
     1561 gtacggctta ctcgaaagtt acgagaagtt tctcagacca gcgctcagta acttggttac
     1621 tgtggtaagg ttcgccacat gcgccctgcc ttgacggact gataacattc gactttcttt
     1681 aactcggcca gggcccatag ctcagaggta agagcaactg actcataatc agtgggtccc
     1741 aggttcgaat cctggtgggc ccagaataag ccagtgagaa agtgatgggt gatggagtga
     1801 taaatacctc cgtagggcga ccgccctcgg tcgcctccgg ccaaagctgt ccgggagaag
     1861 attttatcac ccatcactct atcactaatc actgtagttc cccgtgggca attagctcag
     1921 ctggttagag cgttcggttc acatccgaaa ggtcacaggt tcgaatcctg tattgcccac
     1981 catgcaaaaa tgacgtatac tacaaaaaag gttcccggtg cgtatactta cgtgaacagc
     2041 gaaggacggt aactttaccg gtgcaggcga cgcgtaaatt tcctgtcgtt gaggaacaat
     2101 caaataggaa agaagtattt cggaaaagaa cccacacggc gccgtgtggc cgaaccagtg
     2161 catcccgaaa aaaacgggat gcattttttt tgaggagtgt cctatggcaa ccgccggagg
     2221 tatgatttcc gtgcagaaag atttgcaaat gcttttgacc ctgcagaacg tggactacga
     2281 tcttctggaa ctggagcgct ccaaagacta cctgccggat atgatccgga ccctggaaca
     2341 gcaaacgacc gaggcccagg aaaacctgaa gagcctggaa aaggaacttt tggagcagaa
     2401 tttggaacag aaacggctgg aactggaaac cgccaccgcc cgggacgagc tctcccgtta
     2461 ccagaagcag atgcgggaca tcaagaccaa taaggagtac gacgccctga tgaaggaaat
     2521 cgacaagcgc aagggcacca tcgccgccga tgaagacctg ctcctcaccg tcatgggcac
     2581 cttggaagaa ctgaccgaaa agatcgagga ggccaaaaag cggcaggcgg aagtcaaaaa
     2641 gaccaacgaa gagcagttag tcaatctcaa acagcaactg tcctccatcg gacagaagat
     2701 caaaatgaaa gaagacgagc gcaaaaacat tacggtgcgc atcgaccgga gcaccttttc
     2761 ggtctacgaa cgggtgcgga aggggaaagg cggtcatgcg gtggtggcgg tgcgcaagcg
     2821 tgcctgcgcc ggctgctata agtcgctccc gccgcaaagg gtcgccgaga tccggcgggg
     2881 ggacaccctc attacctgcg acaactgcgg ccggcttttg atctggaccg gcgaagactg
     2941 aacattcgta cactgcttta gaccaacccc ccgggggtag cggaaaccgc ttgcccccgg
     3001 tttttatttt tcctattttt gctctatgga ggggaaacgg atggccactc tgcaacccaa
     3061 gaagatagaa aaagtcggct tgactttcga cgacgttcta ttagttcccg ccaaatcgga
     3121 aatcctgccc cgcgacgtcg acgtctcggt catcatcgcc cgcgacatcc ggctgaacat
     3181 ccccattctc tcggctgcca tggacactgt caccgagtcg cgcctggcga ttgcgctggc
     3241 gcgcgagggc ggcctggggg taattcacaa gaatatgtcg atcgaggcgc aagcggcgga
     3301 agtggaccgg gtcaaacgct ccgaagccgg gatgatcgtt catccgatta ctttggctcc
     3361 ggagcggccc atcggcgagg cgctggaggt gatgcggcgc tacagcattt ccggcattcc
     3421 ggtgaccaaa agggggcggc tggtcggcat cctgaccaac cgcgacctgc gctttgagaa
     3481 aaatttgagc cgcaagatcg gcgaggtgat gaccaaaaag aacttggtca ccgccccgga
     3541 gggaaccgaa ctggaggcgg ccaaagccat tctgcaaaag catcggattg agaaactgcc
     3601 gattgtcgac cgcaggaaaa cgctcaaggg catgatcacg gtcaaagaca tcatgaagca
     3661 gatccagcac ccccacgcct gcaaggataa acggggccgg ctgcgcgtgg ggggagcgg
//
LOCUS       DBNGZD010000410        10515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_898291, whole genome shotgun sequence.
ACCESSION   DBNGZD010000410 DBNGZD010000000
VERSION     DBNGZD010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_898291"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..686)
                     /gene="folK"
                     /locus_tag="AC16GM_10600"
     CDS             complement(141..686)
                     /gene="folK"
                     /locus_tag="AC16GM_10600"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR2924155.1"
                     /translation="MSTAFVGLGSNLGDRLHNLKAAWQELSWLPGTKAVTASSLYETE
                     PVEFADQPYFLNQVVQLETTLDPEELLEELLRIEAHLGRTRTVPKGPRTLDLDLLLFD
                     QLIIETPRLTLPHPALHRRRFVLVPLAEIAPAVLHPILRKKMVALLAELSDQSSVQMY
                     RKREAATAATLSTAVPSSKEG"
     gene            complement(689..1048)
                     /gene="folB"
                     /locus_tag="AC16GM_10605"
     CDS             complement(689..1048)
                     /gene="folB"
                     /locus_tag="AC16GM_10605"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR2924156.1"
                     /translation="MDVIRLHNMTFYGYHGASSAERETGRRFEVDAELFFPVSRAART
                     DRLKNTIDYTKVYTCVEKVILQNHFTLLETVADRLAQSILKNFPIRKVTIRVRKKIPP
                     IPGNLDHIEVELTRSKR"
     gene            complement(1052..2308)
                     /locus_tag="AC16GM_10610"
     CDS             complement(1052..2308)
                     /locus_tag="AC16GM_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR2924157.1"
                     /translation="MIKRVILDKAERLQQLTPDFTYAVAPARARTGKRLVVPQIDLSS
                     ELSDLPPPASVTALLERSPDILMKRRPAEPKTTAEFKGCIAEWFSERYGVTLDPKKEI
                     LPTLGNRAALFGLALAYLNPKEKAALPNPGYPFYRLAVLTAGGLAEYLPLRERNDYLP
                     TLAQLESESPSLRHRLRGQETLRQPKFVFLNYPNNPTGAVADEQFYQNAVRFASDHNV
                     LLINDAVYSELTYDFRPQSLLSPDHAKRVAVEFHSFTFTFGLSRIPLGFAVGNRDVLA
                     TLEALFHTLGSTPDEFSLQIGLAALESATGSIDHLRRTFRARRDLALTRVTSFGWQVR
                     KPLGGPFIWIKLPRSFSSVGFARRILRKAGVVVTPGVYFGEQGEGYVRLSLCKPEAEL
                     TRAFDRIAQTWPLLRPSLKSKTAASV"
     gene            complement(2321..2917)
                     /gene="pyrE"
                     /locus_tag="AC16GM_10615"
     CDS             complement(2321..2917)
                     /gene="pyrE"
                     /locus_tag="AC16GM_10615"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR2924158.1"
                     /translation="MTPTDVLEISKQAGALLTGHFILTSGRHSDVYFEKFAVLQHPAY
                     LEKLAGELARCFDSEKIDLVVGPTTGGMLLAYEVARQWGKRFLFAEPGENGRVFKRGF
                     TLAPGTRVLVVDDVLTTGRSVAEVLALVRSYGATVAGIGLLLDRSEKPLDFGIAYTAL
                     TKVTATSFVPDACPLCQKGLPLTKPGSREQPAKGTAAS"
     gene            complement(2942..3331)
                     /gene="acpS"
                     /locus_tag="AC16GM_10620"
     CDS             complement(2942..3331)
                     /gene="acpS"
                     /locus_tag="AC16GM_10620"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR2924159.1"
                     /translation="MLPPDMVAGVGVDIIEVSRIQAAIERWGERFLKRIYTPGEVAYC
                     RSLERPFESFAGRFAVKEACMKALGTGWTDGVKWRSFEVKNEKSGKPYVVLDGDLDKR
                     INGKKILVSISHTENYATGVAVLSDQP"
     gene            complement(3641..4372)
                     /locus_tag="AC16GM_10625"
     CDS             complement(3641..4372)
                     /locus_tag="AC16GM_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924160.1"
                     /translation="MKELLQDIKTVTGVSGVMILDKRREATHQLLPASLAPEQVKKLA
                     MKLVIVANSIERPGRIDLRFTEGWTILVNLPSGAVLVLTKDSVDFSMLRLVLKKVLAQ
                     LAREVDAPSTQTAHSGAALDSRSIETLFEVTNSIAGRYREVQGPFQVLQHLRKAKEKL
                     LPSHPGLVNFFIDPNGQLLFGRLKEGSVELTVDTFALFLAQFLNYANGPGNKLPPGTL
                     TEWLWEYRERLEGLNFFHALHAVKS"
     gene            complement(4369..5007)
                     /locus_tag="AC16GM_10630"
     CDS             complement(4369..5007)
                     /locus_tag="AC16GM_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924161.1"
                     /translation="MYQPSGLTGQVECGGKTFRLQTEFLERPQPRIVTSVFCEGQVVY
                     KVAAEWDKDLEDTGLQAELDLAIQKQHADAVAKIGAEGERLWDEAAKHPPATQKVPLA
                     VATVQLEEKLKTTPGLVGLIVASYEGKVLNQAELAGPTKSWATLVAELAGFAGLLAKA
                     TRLGSLVEGTAEFPEGKLVVSGQPETVVGIVVKTTTDTQQAKDNLKLALQER"
     gene            complement(5347..5946)
                     /locus_tag="AC16GM_10635"
     CDS             complement(5347..5946)
                     /locus_tag="AC16GM_10635"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR2924162.1"
                     /translation="MPDQITPKPLKPSLLQMDGISARTIEEHEKLYKGYVNKTNEIRG
                     KLATVDLSTANQVYSDLRVLKLELTFAIGGVKNHEIYFDNLGGKGGQPSGDLMAMIKR
                     DFGSFDSWQKDLKATGISARGWAWLAYDHDDGKMFNYLGDAQNTFPIWNATPVVALDT
                     YEHAYFIDYGVNRAAYIDKFFANLDWPAIEKRFAGLKIK"
     gene            complement(6334..7485)
                     /locus_tag="AC16GM_10640"
     CDS             complement(6334..7485)
                     /locus_tag="AC16GM_10640"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR2924163.1"
                     /translation="MTFLRTLLCTKCGQEHDPSRPQNLCPCGGPLYAQYDLVSIAKKL
                     KKSDLPSRPASLWRYRELLPVEDERNVVTLGEGFTPLIKADQLGARLGLENLWIKDES
                     GNPTGSFKARGMAVAISKARELGITKVALPSAGNAGGAAAAYAAKAGIDCTVFVPDDT
                     PKAFVEECQACGAKVRLVSGSIADAGKEMAKEREKEGWFDLSTLKEPYRVEGKKTLGY
                     ELAEQFDWELPDWVIYPTGGGTGLVGMWKAFDELEKLGWIGKKRPKMVTVQASGCAPI
                     VKAFEEKAERTSPWPNPQTRALGLRVPSAVGDFLMLKVLRESNGAAVAVSEEEIEAGQ
                     KSLAQAEGIFACPETGATMAGLKKLVEQSRISQQDSVVVFHTGSGYKYL"
     gene            7569..9593
                     /gene="ligA"
                     /locus_tag="AC16GM_10645"
     CDS             7569..9593
                     /gene="ligA"
                     /locus_tag="AC16GM_10645"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR2924164.1"
                     /translation="MAEKTSGAKAWVALLREKINYHNYRYHVLDKPEISDAEYDRLFD
                     ELVALEKAHPELITPDSPTQRVGAAPAEQFKTVRHTFPMLSLGKASSEEEFLDFHHRV
                     LELSDQPESKIEYVCEPKLDGLAVELIYRDGIFVLGSTRGDGVAGEEVTQNLKTIKTI
                     PLRLLSDGKPVPRLLEVRGEVIIAKKAFWELNRKLEEAGEEPFANPRNFAAGQVRQLD
                     PRMTASRPLEIFAYGIGDLKGEKHRTHYETMQRLKELGLRINREIHLCRKPKDVMDYY
                     DKMLARRDKWDYEMDGIVIKVNDYELQRTLGELSRSPRWAVAWKFPPQQAETVIEEIK
                     VQVGRTGVLTPVAHLKPVHVAGVEVSRATLHNEDEIKKKDIRIGDTVVIERAGDVIPD
                     VVSVVTAKRTGKERHFKMPEKCPVCGSKVERLPGEVAYRCTGLACPAQLKERIAHFAC
                     KGGMDIEGLGFKWIEQFVEKRFISDPADLYFLTKEQLLTLDRMGPKLADNILNAIEKS
                     KRPDLPHLLFALGIRNVGEHLAGVLAREVGSIDNIAEQTIDDLTKIHEVGPIVAQSLY
                     NFFQDRNNQKVLAKLEKGGVVFPVEKRKLKQTVLTGKTFVLTGGLDSFTRDEAKKIIE
                     DMGGRVASSVGKKTDFVLVGKDPGSKFDDARRLGIRTIDEAEFKKMIGKG"
     gene            9597..>10515
                     /locus_tag="AC16GM_10650"
     CDS             9597..>10515
                     /locus_tag="AC16GM_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR2924165.1"
                     /translation="MTPPAKNLSLTAIALPPVAVAGATAFFYFAAPILVPLVCGVFAA
                     YLLGPAVGVLRHIKVPRVVAVFLVMLITILVLLVAGYFAGQQIGQLTGDLPHYWNAAV
                     QWLGQLQAKIAGWLQPKSTAPEPLNPVASLKWENLTSVSKIAFKGLGSVVNFFFGVIL
                     IFFITFFLLLDHERLKERAVRAFGRGNDEVAGKILSEINGQIAGFVAIKFVITVMLAV
                     VITAGLLLFRVKYAFVWGPVAAVLNLIPYIGPVIGLVPPVIVAGIQHQSALAAFWVFV
                     FFVAVQAVESNFLTPRLLSEKVNLNPLAVL"
BASE COUNT         2189 a         2931 c         3147 g         2248 t
ORIGIN      
        1 ggtctgcatg ccggacatcg acatcgccgc ggcgccggcc gacatggtca tcttggaggt
       61 gattttcaca tccgcctgac tttgcgcggc gacgagcagg accacggcga gtgcggcgag
      121 acttcggttt cggatcgaca ttacccctcc tttgagctgg gaactgcggt tgacaatgtt
      181 gccgctgttg cggcttctct cttccggtac atctgtacgc tcgattgatc ggaaagttcg
      241 gccaaaagcg cgaccatttt ttttctcaat atcgggtgga gaacggcggg cgcaatttca
      301 gccaacggca caagtacaaa ccgtctccgg tgcaaggccg gatggggaag ggtcaggcgg
      361 ggtgtctcga taatcagttg atcgaacaag agcaggtcga ggtcaagcgt gcgcgggcct
      421 ttgggtacgg tgcgggttcg gcccaggtgc gcttcaatgc gtaaaagttc ttccagcagc
      481 tcttcgggat ctaaagttgt ctccagttgc accacctggt tcaagaaata gggctggtcg
      541 gcaaattcta ccggctcggt ttcataaaga gaagaggccg tgaccgcctt cgttcccggc
      601 aaccaagaga gttcctgcca ggccgctttt aggttatgca accgatcgcc gagatttgat
      661 cctaaaccga caaaggccgt gctcactttt acctcttgct ccgggtcagt tccacctcga
      721 tgtgatctaa gttgcccgga atgggcggaa tttttttccg cacccggatg gtcaccttcc
      781 ggatgggaaa gtttttcaga atggactgcg ccaggcgatc ggccaccgtt tccagcagcg
      841 tgaaatggtt ttgcaggata accttctcca cgcaggtata gaccttggta taatcaatgg
      901 tgttcttcag ccggtcggtg cgggcggccc ggctgaccgg gaagaacagt tcggcatcga
      961 cctcgaagcg gcggccggtc tccctttcgg cggacgaggc gccgtggtag ccgtagaagg
     1021 tcatgttgtg caggcggatc acatccatcg ttcagaccga ggccgcggtt ttgctcttca
     1081 acgacggccg cagcaacggc caggtttggg cgatgcgatc aaaggcccgg gtcaattccg
     1141 cttcgggctt gcacagggac aggcgcacgt agccttcacc ctgctcgccg aaatatacgc
     1201 cgggggtcac gaccacacca gccttgcgca ggatccgtcg cgcaaagccc accgatgaaa
     1261 agctgcgcgg aagtttgatc cagatgaatg gcccgcccag aggttttctg acctgccaac
     1321 cgaaagacgt tacgcgggta agagcaagat cccgccgcgc gcggaaggtg cggcgcaggt
     1381 gatcgatcga cccggtggcc gattccagcg cggcgaggcc gatctggagc gaaaactcat
     1441 cgggggtgct ccccagcgtg tgaaagagcg cctccagcgt ggccaagacg tcgcggttcc
     1501 ccacggcaaa gcccagcggg atgcggctta gaccaaaggt aaaggtgaag ctgtggaatt
     1561 caactgccac gcgtttggca tggtccggcg acaaaagact ttgcggccgg aagtcgtacg
     1621 tcagttcgga gtaaacggca tcattgatga gcaaaacatt gtgatccgaa gcgaagcgaa
     1681 ccgcattctg gtagaactgc tcatcggcga ctgccccggt gggattgttc gggtagttca
     1741 aaaacacaaa tttcggctgg cggagtgttt cctggccgcg caggcggtgc cgcaggctgg
     1801 gggactcact ttccagttgc gccagtgtgg ggaggtaatc gttccgctca cgcagaggca
     1861 ggtactcggc cagaccgcca gcggtcagaa cggcgagccg gtaaaatgga taccctgggt
     1921 tcggaagggc cgctttttct ttcgggttga gataagctaa agccaggccg aaaagagcgg
     1981 cccggtttcc cagcgtgggc aggatttcct ttttcgggtc gagggtcacg ccgtaacgct
     2041 cggaaaacca ctccgcgatg caccccttga attccgccgt ggtcttcggc tctgcgggac
     2101 ggcgtttcat caggatgtcg ggtgaccgct ctaaaagcgc cgtgacggaa gccggcggcg
     2161 gcaagtcgga gagttcggaa cttaagtcga tctgggggac gaccagccgc tttcccgtcc
     2221 gggccctggc cggtgccacc gcgtaggtga agtcgggggt caactgctgc aaacgttccg
     2281 ctttgtctaa aatgacgcgt ttgatcatgg ggctgctaat tcatgaggca gcagtaccct
     2341 tcgccggttg ttcccggctg cccggtttgg tgaggggtag acccttctgg cagaggggac
     2401 aggcgtccgg cacaaatgag gtggcagtca cttttgtcaa agccgtgtac gcgatgccga
     2461 aatcgagcgg tttttcggag cggtcgagta agaggcctat tccggcgacg gtcgctccat
     2521 acgatctgac caatgccagc acttcggcca ccgagcggcc ggtggtcaaa acgtcatcca
     2581 cgaccagcac ccgggtgccg ggggccaaag taaagcccct tttgaagacc cggccgtttt
     2641 caccgggttc ggcaaagagg aagcgtttcc cccactgccg ggcaacctcg tacgccagaa
     2701 gcattccgcc ggtggtgggg ccgaccacca ggtcaatttt ttccgaatcg aagcaccggg
     2761 cgagttctcc ggccagcttt tccagataag ccggatgttg cagcaccgcg aatttctcaa
     2821 agtagacgtc gctgtgccgg ccggaggtca gaataaagtg gccggtgagc agcgctcccg
     2881 cctgcttaga gatttcgagg acatcagttg gagtcacgat agtcccaagt ctttcattag
     2941 cttaaggttg gtcgcttagc accgccacgc cggtggcgta attttcggtg tgggagatcg
     3001 atacgagtat cttctttcca tttatccttt tgtcgagatc accgtccagc accacgtacg
     3061 gcttccccga tttttcgttt ttcacctcaa acgaccgcca tttcacccca tcggtccagc
     3121 cggtgcctaa ggccttcata caggcctcct tgaccgcgaa ccggccggcg aacgactcaa
     3181 acggccgctc caagctccgg caataagcca cttcgcccgg ggtgtaaatc cgcttcagga
     3241 acctttcgcc ccagcgctct atggccgcct gaatgcggga tacctcaata atatccacac
     3301 cgacgccggc caccatatcc ggtggtagca tacgggaatc gttggcggtt ggcaatccgg
     3361 gtggtatatt aagcccgacg tgggaggaga taatttgaaa aacctcttac aaggataaga
     3421 tgaaggccgc tcccctgagt tgcgcgtacg cgatgacttc ccgcaaggga aagagcgaaa
     3481 gcaaaagacg aagggtggct gtaaaacgcc tttaccgttc tatttgagag ggtttttcaa
     3541 atagagcact cttccccggc aaagtcctcc cgcctcgggc gggaggcttt tttattgctc
     3601 ctcagagtcg gatctgcctg agggggataa ggtccgagtc ttacgacttg accgcgtgga
     3661 gggcgtggaa gaagttgagc ccctccagcc gttcgcggta ctcccagagc cattcggtga
     3721 gggtgcccgg agggagctta ttgcccggcc cgttggcgta gttcaagaac tgggccaaaa
     3781 agagcgcgaa ggtgtctacg gtcagttcta ccgagccctc ttttaagcgg ccgaagagga
     3841 gttgaccgtt ggggtcgatg aaaaagttca ccaagccggg atgggagggg aggagttttt
     3901 ctttggcttt gcgcaaatgc tggagcacct ggaacggccc ctgcacctcc cggtagcggc
     3961 cggcaatgga gttcgtgact tcaaagaggg tttcaatgct tcgggaatcg agcgccgccc
     4021 cggagtgcgc tgtctgcgtt gagggagcgt caacttcccg cgccagctgc gccagaacct
     4081 tcttcagcac cagccggagc atcgaaaaat cgacgctgtc cttggtgagc acgagcacag
     4141 cgccggaagg gaggttcact aaaatcgtcc agccctcagt gaagcggagg tcaatccggc
     4201 cgggcctttc gatgctgttg gccacgatga ccagtttcat agccagtttc ttcacctgct
     4261 ccggcgccag tgaagcgggc agaagctggt gggtggcttc acggcgtttg tccagaatca
     4321 tgacgccgga aacgccggtg acggtcttga tatcctgcag aagttctttc atcgctcttg
     4381 gagcgccagt ttcaggttgt ctttggcctg ctgggtgtcg gtggtggtct tgacgacgat
     4441 gcccaccacc gtttcgggct ggcccgacac gaccagtttg ccctccggga actcggcggt
     4501 accttcaacc agactcccca gccgggtcgc tttggccaga agcccggcaa aaccggccag
     4561 ttcggccacc agggtggccc acgacttggt cgggccggca agttcggcct gattcaaaac
     4621 cttgccttca tacgaggcca cgatcaagcc caccaggccg ggagtggtct tcagtttttc
     4681 ttcgagctgg acggtagcga cggccagcgg cactttttgg gtcgcgggcg ggtgcttggc
     4741 tgcctcatcc cagagccgtt cgccctcggc tccgattttc gccaccgcgt cggcgtgctg
     4801 tttctggatg gctaaatcga gttcggcctg aagcccggtg tcttccaagt ccttatccca
     4861 ttcggccgct accttgtaga ccacctggcc ttcacagaat accgaagtga caatgcgcgg
     4921 ctgggggcgc tccagaaatt ccgtctgaag acggaaagtc ttcccgccac attcgacctg
     4981 gccggtcaaa ccggatggtt gatacatagt ctctccctgg ggtgggtacc caccctttct
     5041 ctataaacat cggctgattg gggaaatggt acagccggtc gaaagcagaa gtcgggctca
     5101 gagacgaggc cgactaccgg actaagtgta gggatggacc cttacgttcg tccgaggctt
     5161 cggtcaggct gggaagtccc gccacgggcg ggactgggaa gcctgaccta tgggaggggg
     5221 atatcttgat tttccatagc cgaggcttcc agcctcggaa taggtggggc aaacagaacc
     5281 gcccttaagg gcggttctga aaacaactac cccagtgtgc tatcgggtga acctaaaggt
     5341 tcacccctac ttgatcttca accccgcgaa gcgtttttcg atggcgggcc agtccaagtt
     5401 ggcgaagaac ttatcgatgt aggccgcacg gttgacgccg tagtcgatga agtaggcgtg
     5461 ctcgtaggtg tccaaggcca caaccggcgt ggcgttccag atggggaaag tgttctgggc
     5521 gtcgcccagg tagttgaaca ttttcccgtc gtcgtggtcg taggccagcc aggcccagcc
     5581 gcgagccgaa atcccggtgg ccttcagatc tttctgccag gaatcaaacg agccgaagtc
     5641 gcgcttgatc atcgccatca ggtcgccgga gggctggccg cccttgccgc ctaagttgtc
     5701 gaagtaaatc tcgtggttct tcaccccgcc gatggcgaag gtcagctcga gtttcagaac
     5761 ccgcaggtca ctgtagacct gattggcggt cgataagtcg acggtcgcca gtttgcctct
     5821 gatttcgttg gtcttgttga cgtagccctt gtagagtttc tcgtgctcct cgatggtcct
     5881 ggcggagatg ccgtccatct gcaggagcga gggcttgagc ggtttggggg taatctggtc
     5941 aggcattgtt tgactccttt ctgcgatttg tgcgagcgat tcggttgatc cgacggtcaa
     6001 aagcgccgca gcggaagctg cgccgccaac taagacagtg agggcttcgc gccgggtata
     6061 ggttgattgc gactgcgcgt ccatccgtga acatcacctc aaggcgtaaa ggaaagcgga
     6121 agaaatatga cagaatcagt cggtttgtca actgacacag gggagccgac taaatgttca
     6181 gtcaggctgg aagtcccgcc aagagcgagc gggactaaaa gcctgaccca tggaagacaa
     6241 atcgttcctc atagccgagc ttctcggtac gagccgtatt ggccagcctc agcacagccg
     6301 gcctcaaagc aatacttgaa tgccaactga ttttcaaaga tacttgtatc cactcccggt
     6361 atgaaagaca accacgctat cctgctggct aatccgcgat tgctcaacca gtttcttcaa
     6421 tcccgccatg gtcgcgccgg tttccggaca ggcgaagatc ccctcggctt gcgccaacga
     6481 cttctgaccg gcctcgattt cctcttcaga aacggcaacc gcagcgccgt tgctttcacg
     6541 gagaaccttg agcatcaaga aatcacccac cgccgaagga acgcgcaggc cgagcgctct
     6601 ggtctggggg ttgggccagg gggaagtgcg ttcggctttt tcctcaaatg ccttcacaat
     6661 cggggcacag ccggaggctt gcacggttac cattttcgga cgttttttac caatccagcc
     6721 gagtttttcc aattcgtcga acgctttcca catccccacc aagccggtcc cgccgccggt
     6781 ggggtagatg acccagtcgg gcaattccca gtcgaattgc tcggccagtt cgtaccccag
     6841 cgtcttttta ccttcgaccc ggtacggttc tttcaaagtg gagagatcaa accagccctc
     6901 tttttctctc tctttggcca tttcctttcc ggcatcagcg atcgatccgg aaacgaggcg
     6961 gactttggct ccgcaggcct ggcattcctc tacgaacgct ttgggcgtgt catccggtac
     7021 aaagacggtg cagtcgatcc cggctttcgc cgcgtaggcc gctgcggcgc ctccggcatt
     7081 gcctgcggat ggaagtgcga ctttggtgat tcccagttcc ctggccttcg aaatcgccac
     7141 agccatcccc ctcgctttga acgagccggt gggattacct gattcatctt tgatccagag
     7201 attttcaagg ccgaggcggg cacccagctg gtcggctttg atgagcggcg tgaatccttc
     7261 accgagagtc acgacattac gctcgtcttc aaccggcagg agttcccggt aacgccagag
     7321 agaggcgggg cggctcggca aatcactttt tttgagtttt ttcgcaatcg aaacaagatc
     7381 gtactgagcg tagagcgggc cgccgcaagg gcagaggttt tgggggcggg aaggatcgtg
     7441 ctcctggccg cacttggtgc agagcagagt tctgaggaag gtcatgaagt cgagaatata
     7501 ctccggctgt cattgcggct caatgtttga caccctgtcg agcggctgtt aattactgtt
     7561 agattccaat ggctgagaaa acttcgggtg ccaaggcgtg ggtcgccctg ctgcgcgaga
     7621 aaatcaacta tcacaactac cggtaccacg tcctggacaa accggaaatc tccgacgccg
     7681 aatatgaccg cctctttgat gaactggtcg ctctggaaaa ggcccaccca gaactaatca
     7741 caccagattc acccacccaa agggtcggtg cggcgccggc ggagcagttc aaaacggttc
     7801 gtcatacgtt cccgatgctt tctttgggaa aggcttcttc ggaagaagag tttcttgatt
     7861 tccaccaccg tgtgttagaa ttatccgacc agccggagag taaaatcgag tacgtctgtg
     7921 aaccgaagtt agacgggctg gcggtggagt tgatataccg cgacggtatc ttcgttttag
     7981 gttccacccg gggcgacggg gtggcaggcg aggaggtgac gcaaaatctc aagacgatca
     8041 aaaccatccc cctgcgcctc ctctccgacg ggaagccggt tccgagattg cttgaagtgc
     8101 gcggcgaggt gatcatcgcc aaaaaagcgt tctgggagtt gaatcgaaaa cttgaggaag
     8161 cgggagagga gccgtttgcc aatccgcgca acttcgccgc gggccaggtg cgccagctgg
     8221 acccccggat gaccgccagc cggcccttag agattttcgc ctacggcatc ggcgacctga
     8281 aaggcgaaaa gcaccggacg cactacgaga cgatgcaacg gctcaaagaa ttaggactga
     8341 ggatcaatcg tgagatacac ctctgccgca agccgaagga cgtgatggac tactacgaca
     8401 agatgctcgc gcggcgggat aagtgggact acgaaatgga cgggatcgtc atcaaagtga
     8461 acgactacga actccagcgc acgttggggg agttatcgcg ctcaccgcgc tgggcggtgg
     8521 cctggaagtt cccgccccag caagccgaga cggtcattga ggaaatcaaa gtgcaggtgg
     8581 ggcggaccgg cgttctaacg ccggtggcgc atctgaagcc agtgcacgtg gccggggtgg
     8641 aggtaagccg ggcgacgctc cacaacgagg acgaaatcaa aaagaaggac attcgcatcg
     8701 gcgacacggt ggtgatcgag cgggccggag acgtgatccc cgacgtggta tcggtcgtca
     8761 ccgccaagcg caccggcaag gaacgtcatt tcaaaatgcc ggagaaatgc ccggtctgcg
     8821 gctccaaagt cgagcggctg ccgggagagg tggcctaccg ctgcaccggt ctggcctgcc
     8881 cggcgcagct gaaggagcgg attgctcatt tcgcctgcaa aggtggaatg gatatcgaag
     8941 gtctgggttt taagtggata gagcagttcg tggagaaaag attcatctcc gatccggcgg
     9001 atctctattt cctcaccaag gagcaactcc tcacgctcga ccggatgggt cccaagttgg
     9061 cggacaacat cctcaacgca atcgagaaaa gtaaaaggcc cgacctgccg catttgctgt
     9121 ttgctctggg aattcgtaac gtgggcgagc atctggccgg ggttttagcc cgcgaggtgg
     9181 ggtcaatcga caatatcgcg gaacaaacga tagatgactt gacaaagatt cacgaagtcg
     9241 gaccgattgt agcacagagc ctctacaatt tcttccagga cagaaacaac cagaaggttt
     9301 tggcaaaatt agaaaagggg ggagtcgtct ttcccgtcga gaaaaggaag ctcaagcaaa
     9361 ccgtcctcac gggcaaaacg tttgtcctca ccggcgggct cgattcgttc acccgcgatg
     9421 aagcgaaaaa aatcatagag gatatgggcg ggcgggtggc ttcctcggtg ggcaaaaaaa
     9481 ccgactttgt gttagtgggc aaggaccccg gctccaagtt cgacgatgcc cggcggctgg
     9541 gaattcgcac catcgatgaa gcagagttca agaaaatgat cggaaagggg taagctatga
     9601 ctcctcccgc caagaatttg tccttgaccg ccatcgcgct ccctccggta gcggtggccg
     9661 gggccacggc gttcttctac tttgcggcgc cgattttagt tccgctcgtc tgcggcgttt
     9721 tcgcggcgta ccttctcggt cccgccgtgg gggtgctacg ccacatcaaa gttccccgag
     9781 tggttgccgt cttcctggtc atgctgatta ccatactggt tcttttggta gcaggttact
     9841 tcgcgggtca gcagatcggg caattaaccg gcgatttgcc acattactgg aatgccgcgg
     9901 tccagtggct cggccaactc caggcaaaaa tagccggctg gttacagccg aaaagcacgg
     9961 cacctgagcc gctcaatccg gttgcgtcgc tcaagtggga aaatctgact tcggtgagca
    10021 agatcgcctt caaggggctg ggttcggtgg tgaatttctt cttcggggtc atcctcattt
    10081 tcttcatcac gttctttttg ctcttggacc acgagcggct caaggagcgc gccgtccgcg
    10141 cctttggccg ggggaatgac gaggtggcgg gcaaaattct ttccgaaatc aacggccaga
    10201 ttgccggctt cgtcgcgatc aagttcgtca tcacggtcat gctggccgtg gtcatcaccg
    10261 ccggactttt gctttttcgg gtgaaatacg ccttcgtttg gggccccgtg gcggcggtcc
    10321 tgaacctgat tccctacatc ggcccggtga tcggcctggt gccgcccgtg attgtggccg
    10381 gcattcagca ccagtcggcc ttggcggctt tctgggtgtt cgtcttcttc gttgcggtcc
    10441 aggccgtgga gagtaacttt ttgaccccga ggttactcag cgaaaaggtg aacttaaatc
    10501 ctctggcggt gctcg
//
LOCUS       DBNGZD010000411         5690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1662117, whole genome shotgun sequence.
ACCESSION   DBNGZD010000411 DBNGZD010000000
VERSION     DBNGZD010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1662117"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="AC16GM_10655"
     CDS             <1..639
                     /locus_tag="AC16GM_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924166.1"
                     /translation="SKVLENKLPSQSRRLFSSLDADTILNILDSMGEKPPEEWLGLLD
                     VSYSAVSWRDFIGKYFIELESGNLSNNEFEDRLATLLGSLGFIIDQKGHVLVGEYPDG
                     VALMDHELGLVYDCKNTDNFKPSAEDERAIMKYLEDERKAKNVKNLYPAFIAKSFVEW
                     GKKNTHYFSVTPLLYLSFKKIQMGPKFSLLPMRKMLDNFQALSQDNIDKEWR"
     gene            680..1684
                     /gene="sucD"
                     /locus_tag="AC16GM_10660"
     CDS             680..1684
                     /gene="sucD"
                     /locus_tag="AC16GM_10660"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR2924167.1"
                     /translation="MSILVDKSTRLLVQGITGRDGSFHTKQMLDYGTKVVAGVTPGKG
                     GILVHGVPVFNTVSEAKEATDANTSVIYVPAAFAADAIYEAADAGLNLVVCITEGIPI
                     SDMLRVYNYLKTTGTRLVGPNCPGLITPGATKVGIIPGHITLPGPVGIVSRSGTLTYE
                     IIYNLTLAGVGQSTCLGIGGDPIIGTSFVDVLALFQADPQTKAVVLIGEIGGTDEEEA
                     AKFIRQEMTKPVVGFIAGITAPPGKRMGHAGAIIAGGAGTAKEKIEAFRAVGVRVAES
                     PDQVVPLVKERLHMTAEKKPGTVRARRKPARTAIPKPPARTRKQSRMVVAAAHRVRKA
                     "
     gene            1698..2108
                     /gene="ndk"
                     /locus_tag="AC16GM_10665"
     CDS             1698..2108
                     /gene="ndk"
                     /locus_tag="AC16GM_10665"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR2924168.1"
                     /translation="MAERTLFIIKPDAVGRNLIGKIIARIEEARFRVRNLKMVRLTEA
                     EARRFYYVHEGKPFLPELVQFMSSGNVVPMVLEKENAVSGLRTLIGATDPKEAAPGTI
                     RADFATDKGKNAVHASDSLESAKFEIDFFFGNER"
     gene            2155..3081
                     /gene="mdh"
                     /locus_tag="AC16GM_10670"
     CDS             2155..3081
                     /gene="mdh"
                     /locus_tag="AC16GM_10670"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR2924169.1"
                     /translation="MKKKVAVIGAGNVGASCALYIAEENLADVVLVDIIEGVPQGKGL
                     DLTQAGPIRGYDCTITGTNDYAQIAGSQLVVVTAGLPRKPGMSREDLITMNAEIVGKV
                     MDGVVKYAPEAFVLVVSNPLDIMTYHAWKKSGFPHQRVFGQAGILDSIRFRSFVAAEL
                     GVSMSDTQALVLGGHGDTMVPLPRYTTVAGIPITELLPKEKVDALSKRTRDGGTEIVA
                     LLKTGSAYYAPAAASVAMVDAVLSDRKRFVAASAYVTGQYGLSDLYIGVPVLLGAKGV
                     EKVIELKLQPDELAALQKSASIYKDMLKVLKY"
     gene            complement(3371..4108)
                     /locus_tag="AC16GM_10675"
     CDS             complement(3371..4108)
                     /locus_tag="AC16GM_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR2924170.1"
                     /translation="MKKTVAIILAAGKGTRMKSPLPKVLHRVGHRPMVWHVIQAARKA
                     DIREIIVVVGFQALRVERALANTDVEFVHQEPQLGTGHAVMVARPLLTHFHGTILVLA
                     GDVPALSPQTIRALVEEHQAERAVATVLTAIWDDPSGYGRVIRTPKGLVKKIVEDGDT
                     TARQKKIREVNTGTFCFQADPLWRALARIQNENRQAEYYLTDVIEIFARRNRRVLAVT
                     APDPWETMGINSRAQMAEVERLFFGGE"
     gene            complement(4110..4466)
                     /gene="panD"
                     /locus_tag="AC16GM_10680"
     CDS             complement(4110..4466)
                     /gene="panD"
                     /locus_tag="AC16GM_10680"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR2924171.1"
                     /translation="MLLECCKAKIHHAAVTEANLRYEGSITIDGSILKAAGILPFERV
                     QVVNVNNGERFDTYVIAGRANSRVICLNGPAARLGQVGDRVIIIAYCLLEQDEAKRHQ
                     PRIVILDDRNRIKKRN"
     gene            complement(4479..5330)
                     /gene="panC"
                     /locus_tag="AC16GM_10685"
     CDS             complement(4479..5330)
                     /gene="panC"
                     /locus_tag="AC16GM_10685"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR2924172.1"
                     /translation="MKIITSVRQMQNVSLALKEKRRSIAFVPTMGYLHAGHLSLIRRA
                     ASLGDVVVVSIFVNPAQFGPADDFARYPRDLKRDTDLAKTAGCDILFVPATEKIYPPD
                     FQTCVQVERVSRGLEGERRPGHFRGVATVVAKLFNIVQPDIAIFGQKDAQQAVVIKQM
                     VRDLNLPVKIVVAPTVREKEGLAHSSRNVYLTPEERKQAPVLYQSLREAKKLVLQGER
                     NVPKIQTQITEMIRSQSGAEIEYVALTDAPTLEPIQNLSGKVLISLAVRFGKTRLIDN
                     IAVTIPK"
     gene            complement(5353..>5690)
                     /locus_tag="AC16GM_10690"
     CDS             complement(5353..>5690)
                     /locus_tag="AC16GM_10690"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR2924173.1"
                     /translation="QGKDAASQEYLQESARALEKAGCFSLVLEGIKSDVAKKITEAVA
                     IPTIGIGAGPHCDGQVLVVNDILGLYETFKPKFVRQYANLAVEIRGACQKFKDDVKSK
                     NFPSSDESY"
BASE COUNT         1285 a         1551 c         1617 g         1237 t
ORIGIN      
        1 agcaaggttt tagaaaacaa actcccatct caaagcagaa gattgttttc ctcgctggat
       61 gcagatacga ttctcaatat actcgattca atgggagaga aacctccaga agagtggtta
      121 ggtttactcg atgtaagtta ttcagccgtc tcatggagag attttattgg caagtacttc
      181 atagagcttg aaagtggtaa tctcagtaat aatgaatttg aagaccgtct agccacgtta
      241 cttggttcgc ttggttttat catagaccaa aaaggacatg tgttagtggg agagtatcct
      301 gatggggttg cattaatgga ccatgagctg gggctggtct acgattgcaa gaatactgac
      361 aattttaagc cgtctgcgga agatgaaagg gcgataatga agtaccttga agatgagaga
      421 aaggcaaaaa acgtaaagaa cttatatccc gcctttattg ccaaaagttt tgttgaatgg
      481 ggcaaaaaaa atactcatta cttttcggtc acaccgttac tctatttatc tttcaaaaag
      541 atacaaatgg gacctaagtt cagccttctt ccaatgagaa aaatgctgga caatttccaa
      601 gcattaagtc aagataatat cgataaagaa tggagatagt tgaacatata tattaaacta
      661 tttctaagaa acgaaaccga tgtccatctt agttgacaaa tccacccgcc tgctcgtcca
      721 gggcatcact ggccgggatg gctctttcca caccaagcag atgctcgact acggcaccaa
      781 agtcgtggcc ggggtgacgc cgggcaaggg cggaattttg gttcacggcg taccggtttt
      841 caatacggtc agtgaggcaa aagaggcgac cgatgccaac acttcggtca tttacgttcc
      901 tgccgctttt gccgctgacg cgatttatga agccgccgac gccgggctca acttagtagt
      961 ctgtatcacc gagggaattc cgatcagcga catgctgcgc gtctacaatt atctgaaaac
     1021 aaccggaacg cgcttggtcg ggccgaactg tcccggctta atcacccccg gcgccaccaa
     1081 agtgggcatc attcccgggc acatcaccct tcccggcccc gtggggatcg tctcgcgctc
     1141 cggcacgctg acgtatgaaa tcatctacaa tctcacgctg gcgggggtag gacagtccac
     1201 ctgcctcggc atcggcggcg atccgatcat cgggacgagt tttgtggatg ttttagccct
     1261 cttccaggcc gatccgcaga ccaaagcggt ggttttgatc ggcgaaatcg gcgggaccga
     1321 tgaggaagaa gcggccaagt ttatccggca ggagatgacc aaaccggtgg taggattcat
     1381 cgcgggtatc accgcgccgc cgggaaagcg gatggggcat gcgggagcga tcattgccgg
     1441 tggagcggga acggccaagg aaaagataga agcgttccga gcggtcggcg tacgggtggc
     1501 cgaaagtccg gaccaggtgg tgccgctggt caaagagcgc ctgcacatga ccgccgaaaa
     1561 gaaacccggt acggttcgcg ccagacgcaa accggcacgg accgccattc ccaaacctcc
     1621 cgcccgtacc cggaagcagt cccgcatggt cgtggcggcg gcgcaccggg tgcgtaaagc
     1681 gtaaaaggac aaggacagtg gccgaacgaa cattattcat catcaagccc gacgcggtcg
     1741 ggcgcaacct cattggaaaa atcatcgccc gcatcgaaga ggcgcggttc cgcgtcagaa
     1801 acctgaaaat ggttcgcttg actgaagcgg aagcccggag gttctactac gtccacgaag
     1861 gcaagccgtt cctgccggaa ttagtgcagt tcatgtcttc cggaaatgtg gtgccgatgg
     1921 ttttagagaa agaaaacgcc gtcagcggcc tgcgcaccct gatcggcgcc accgatccca
     1981 aagaagccgc gcccgggacg atccgggctg atttcgccac agacaaaggg aaaaacgcgg
     2041 tgcacgcctc cgattcgctg gagtcggcga aatttgaaat cgatttcttc tttggcaacg
     2101 agaggtaatt tttttgtgtt cgatcgtgaa acctttcaca aaccggaggg aaagatgaaa
     2161 aagaaagtgg cagtcatcgg ggcggggaat gtcggcgcct cctgcgcatt gtacatcgcg
     2221 gaagaaaatt tagccgatgt cgtcttagtg gacataatcg agggagtgcc ccaggggaag
     2281 gggctggact tgacccaggc cgggccgatc cggggctacg actgcaccat caccggcacc
     2341 aacgactacg cgcagattgc cggctcgcaa ctggtggtgg tgacggccgg cctgccgcgc
     2401 aagccgggga tgagccggga agacctgatc accatgaacg cggagatcgt cgggaaagtg
     2461 atggacgggg tcgtcaaata cgccccggag gccttcgtgc tggtggtctc caatccgctc
     2521 gacatcatga cctaccacgc ctggaaaaaa tcggggtttc cgcaccagcg cgtcttcggg
     2581 caggccggga ttttagattc gatccgtttc cgatcattcg tggcggcgga attgggcgtt
     2641 tcgatgagcg acacgcaggc cctcgttttg ggcgggcacg gcgatacgat ggtgcccttg
     2701 ccgcgctaca ccaccgtggc cgggattccc atcaccgaac ttttgccgaa agaaaaagtt
     2761 gatgcgctct cgaagcgaac ccgcgacggc ggcactgaaa tcgtcgcctt acttaaaacc
     2821 ggcagcgcct actacgcacc ggcggcggcc tccgtcgcca tggtggacgc cgtcctttcc
     2881 gaccgcaagc gtttcgtcgc ggcttcagcc tacgtcaccg gccagtacgg gctttccgat
     2941 ttgtacatcg gtgtgccggt gcttttaggc gccaaaggcg tggagaaggt tattgaactg
     3001 aagttacagc cggatgaact ggcggcattg cagaaatcgg catcaattta caaagatatg
     3061 ctgaaggtgc tgaagtattg agtggttgaa ttccgggaaa agagaaccaa aggcgacccc
     3121 gcctttggcg gggccgaggt tagaaaccac ggctacgaac gtcgacaggc gggtgccgag
     3181 gctgggaagc ctcggctatg gaggaaaatc tcgtttccca tgggtcaggc ttccagcctg
     3241 accaaaatct tcgaatttgg ggtcacctcc cttattgtcc ttcccgtgtg gcccaagaaa
     3301 aacgacgctc tttcagcgcc gccgaggttg gtcggtacga ctctgacgag aaacctcggc
     3361 tacgactgaa ttattcgccc ccaaaaaaga ggcgttcgac ttcggccatc tgggcccggg
     3421 agttgatgcc catcgtttcc caggggtcgg gtgcggtcac cgccaaaacg cgccggttcc
     3481 gccgggcaaa gatttcgatg acgtcggtga ggtagtactc cgcctgccgg ttttcgttct
     3541 ggatccgggc gagcgcccgc cagagcggat cggcctgaaa gcagaaggtg ccggtattga
     3601 cttcgcggat cttcttctgc cgcgcggtgg tgtctccatc ctcgacgatt tttttcacca
     3661 gccctttggg cgtccggatc acccggccgt agccggaagg atcgtcccag atcgccgtga
     3721 gtaccgtggc caccgcacgt tccgcctgat gttcttccac cagcgcccgg atcgtctgag
     3781 gggagagcgc cggcacgtcg cccgccaaca ccaaaatcgt cccgtgaaag tgggtcaaga
     3841 gcggccgggc caccatgacg gcgtggccgg tgcccagctg cggctcctgg tggacgaatt
     3901 cgacgtcggt attggccagc gcccgttcga cgcggagcgc ctgaaagcct accacgacga
     3961 tgatttccct gatatctgcc ttgcgcgcgg cctgaatgac gtgccagacc ataggccggt
     4021 gccccacccg gtggagcacc ttgggcaagg gcgacttcat ccgggtgcct ttgccagcgg
     4081 ccaggattat ggcgacggtt ttcttcatct cagtttcgct tcttgattcg gttgcggtcg
     4141 tctaaaatca cgatgcgcgg ctggtgtctt ttcgcttcgt cctgttccag caggcagtag
     4201 gcaatgataa tcacccggtc acccacctgg ccgagacggg cggccggccc gttcagacag
     4261 atgacccggg agttggcccg gccggcgatg acgtaggtat caaaccgctc gccgttgttt
     4321 acgttaacca cctgcacccg ctcgaagggc agaatgcccg ccgctttcag gattgagcca
     4381 tcaatggtga tactcccctc gtaccggagg tttgcttcgg taacggcggc gtggtggatt
     4441 ttggccttac aacattctaa aagcatcgtc ttttgatctc acttcggtat cgtgaccgca
     4501 atattatcaa tcaaccgcgt tttcccaaag cggacggcaa gcgaaattaa tacttttccc
     4561 gagagatttt gtatgggttc cagcgtaggg gcgtcggtga gcgccacgta ttcgatttcc
     4621 gctccggatt gtgagcgaat catttcagtt atttgcgtct gtattttagg cacgttgcgc
     4681 tcaccttgca gaacgagttt tttcgcttcc cgaagcgact ggtagagcac cggcgcctgc
     4741 ttccgctcct ccggggtgag gtaaacgttg cgcgaactat gagccaggcc ttctttttcg
     4801 cggacggtcg gggcgacgac gattttcacc ggcaggttta agtccctcac catttgcttg
     4861 atcaccaccg cctgctgggc gtctttctgt ccaaaaatgg caatatccgg ctggacgata
     4921 ttaaacaact tcgccaccac cgttgccacg cctctgaagt gccccggacg cctctcgcct
     4981 tcgagacctc gcgacactct ctcaacttgg acgcaggttt gaaaatcggg aggatagatc
     5041 ttctcggttg cgggaacaaa aagaatatca caaccggcag ttttcgccag gtcggtgtcc
     5101 cgctttaaat cacgcgggta gcgggcgaaa tcatcagccg ggccgaactg ggcgggattg
     5161 acaaaaatcg agactacgac gacatcgccc agactggcgg cacggcgaat caggctgaga
     5221 tgtcctgcgt gcaggtagcc catcgtcggt acgaaagcaa tcgaccggcg cttttcttta
     5281 agcgccagtg acacattttg catctgacgg actgaagtaa tgattttcat ggaaggaaaa
     5341 aacctggagg ggttagtagc tctcgtccga ggagggaaag tttttggatt tcacgtcgtc
     5401 cttgaacttc tggcaggcac cgcggatttc cacagcgaga ttggcgtact gccggacgaa
     5461 tttgggtttg aacgtttcgt acagtcccaa aatatcgttc accactaaaa cctgcccgtc
     5521 gcagtgaggc ccggcgccga tgccgatggt aggaattgcc accgcctccg tgattttttt
     5581 ggccacgtca ctttttattc cctcaaggac taaagaaaag caacccgctt tttccagtgc
     5641 ccgcgcactc tcctgcaaat actcctgcga ggccgcatct ttgccctgaa
//
LOCUS       DBNGZD010000412         9937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1523453, whole genome shotgun sequence.
ACCESSION   DBNGZD010000412 DBNGZD010000000
VERSION     DBNGZD010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1523453"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..919
                     /gene="bshB1"
                     /locus_tag="AC16GM_10695"
     CDS             212..919
                     /gene="bshB1"
                     /locus_tag="AC16GM_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HIR2924174.1"
                     /translation="MKRKEFDVIAIGAHPDDVEVGTGGVMIKLHQLGYRTGLVSLTRG
                     EMGTGGTAAIRRKETRRGAAILGAQLLATLNFGDCKLVDSFTNRLKVARLLRKFRPRV
                     VLAPFWDGGHGKRQGHPDHLACGRIVMNAVNFAGLKKMPLREEPHQVKALFHYFLPAG
                     LMPTFVVDITAEFPRWREALKAHRSQFLNPEKSLDYLWFLESMARTYGNYIRTRYGQG
                     FVIGEPFKIDDLTLLAG"
     gene            923..2911
                     /locus_tag="AC16GM_10700"
     CDS             923..2911
                     /locus_tag="AC16GM_10700"
                     /inference="COORDINATES: protein motif:HMM:NF020086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HIR2924175.1"
                     /translation="MSPRAKKTAAKTDPTTVRPLPMTPEELHLRGWDSLDILLITGDA
                     YIDHPAVHVALIGRLLESHGFKVGVIAQPDWKNLSDFSRLGRPRLAVYVTGGQNEPLI
                     THFLAKDKPLLPDAWSPGGSPVKRPARAVMTYAQRARQAFPGLPVIIGGVEASLRRLA
                     YFDPWNNAVRRSILFDAKADLLIYTPGEFEMLAVAQRLAQGEPIEKITNLPGTTVKRR
                     RLPSRGPVRELFSYDEVKTDKLKYLEAFSDQAVQRAPGAGFPVAQLTGDRYVVVNPPP
                     ARTTAELDLLYALPFTRLPHPSYGEREKIAVAEALRFAIVSHRGCVCGASFCQHEPYQ
                     GSQIQSRSEQSILDEAVSFASDPAFQGMISDLSGPYANHYGSDFISGNGQNQSRTVFA
                     EQGGSDGDGNGGGSTAALTDLRAAEMPFGAQQAFTLLLQRLQKLPGINRVLLTSPLRL
                     EWAAADPDFLNQVATHHVQNHCRIMPGHVTKAVNRLMRRPGVEMLDKFLALFLDLSQK
                     AKKEQFIQAHFIINHPGCRTFDMVELAAYSRKRRLTLNWFWDFSPVPLTASGVMAYTA
                     LDPETRQPLYVPREAKDRLIQKALVRYLDPGSHYLVKKALYACNRADLIGSSSLCLIS
                     PKPKPRVMRKPVQARPDTHLPPRPAGTNRLDAFRHRSY"
     gene            2942..3454
                     /locus_tag="AC16GM_10705"
     CDS             2942..3454
                     /locus_tag="AC16GM_10705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR2924176.1"
                     /translation="MSKIKGDLVEVFLFYRRGKRQRILLLKRSAGSNWGGLWQGVSGK
                     IKKGESAIRACLREIGEETNLSVKRLYALDLVNQFYAPRTDTVYLGPFFAAEVTTPES
                     LRLSREHDACRWATVQQALRLVIWEGYRRALLRVQNDLFSKNAVDRNFLVYEPPESKQ
                     KGSKARLLRI"
     gene            3540..4919
                     /locus_tag="AC16GM_10710"
     CDS             3540..4919
                     /locus_tag="AC16GM_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2924177.1"
                     /translation="MKKWIWIIVGVVAIAAVVVYAATRQNGKNDKTQSATGKIVKPFR
                     GDLVVSITATGQIEPIDAVEIRSKASGEIIRLPIDDGVSVKKGDLIAQLDQTTAQNDY
                     EQAKADFGVAEATLNQREKELNRVKGLFEKNLVSQQDLDNAQLAYEQARSQFVRTKTT
                     LSTMEERLKDTVVRSPISGVVLQKNVEAGQIISSAVSSVSGGTLIATVAGMDKVYVVA
                     DVDETDIGKVAIGQGAKVVADAYPDEEFHGRVLKIAPLAKVAQNVTTIDVTAEVDNAN
                     HLLKAGMNANVDIVTAEARNALLVPVEALVEPAEAKMAMGNGAPGPGQSAEAQTKPPA
                     DAGGQTREISPERRERFRQMMESGQVTPEMMAQWQKRRAARRDTSAVNPEKPHARTKV
                     VYVLEKGQPTPRKVTVGLSNLDNAQIVDGLTEQDEIVVQSSSQLMRDREEMKQRMQRF
                     TGLPGVKKQ"
     gene            4971..6191
                     /locus_tag="AC16GM_10715"
     CDS             4971..6191
                     /locus_tag="AC16GM_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2924178.1"
                     /translation="MAIAWSEALGVSLGALRANRIRSFLTMLGIIIGVGAVITMIALG
                     QGAKKAVQDRIESLGANLLFVRPGAPFRGGVRFESGSNVRLTDKDVEAIQTKVRGLEI
                     MVPETGRRAQVKYGNQNWNTQVSGTIPEYQFVRNSPCMVGNYFTKNDVATRQRVAVLG
                     HEVWNNLFNGQDPVGKEVRINNISFTVVGLLAEKGQAMMFNQDDQILIPITTAQKRLF
                     GIDFLTNVNVKVADEAVMEQAALDIEQVLRRQHRLKPGEENDFNIRSQADILSTLSET
                     SQTFTTLLASIALVSLLVGGIGIMNIMLVSVTERTREIGTRIAIGAKKRDIMNQFLIE
                     ALLLSLCGGAIGAAAGIGGSVLLSKLANWNTLISVSSIFLAFLFSAAVGIFFGLYPAR
                     KAARLNPIEALRYE"
     gene            6252..7229
                     /gene="ilvC"
                     /locus_tag="AC16GM_10720"
     CDS             6252..7229
                     /gene="ilvC"
                     /locus_tag="AC16GM_10720"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR2924179.1"
                     /translation="MRAFYDSDADLSLLKGKTVAVIGYGSQGRAQALNLRDSGAAVVV
                     GLPQTSASRKNAREDGLAVSSTEEAGKRGDFISLLVPDQLHQKIFQKSIRPYLTPDKT
                     LVMAHGYSVHFRLIQPPTTVDTLLVAPLGPGEILRERFTRGKGIACLLAVRSKNQTSA
                     WTKAKTYARAIGCTRMGAIATTFREEAVGDLFGEQAVLCGGLSQLLLAGFETLVKNGI
                     NPESAYLECVQQIDLIMALIKRHGLRGMFQRISATAAYGAARSGRKVIDHRVRRSMQQ
                     IFTDIASGRFARNWQKTFTDRHKVERLLETASAPVFERAHKRVQALLRE"
     gene            7233..9317
                     /locus_tag="AC16GM_10725"
     CDS             7233..9317
                     /locus_tag="AC16GM_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HIR2924180.1"
                     /translation="MYFLNPFFLFATLFALLPLLIHLFNRQKVKKIEFSSVAFLKAME
                     RTKIRRLKLRQLWLLLLRTLIILLVALAFARPAVRGKFGRKVGSHAKTSAVIILDNSA
                     SMGLETKNGSLFELAQKKTQKIIDLFAEGDETALVLTNGGGQPTLTHNFRRLSRDLAE
                     AQLSSGATALDRTIASAARLLSSSKNLNREIFLFSDLKSPAGAISDTALSHFHGKLFL
                     FPVKAADYENVSLQEIDFGRQLITLGVPFQMTAKVQNQSPRPEEGLLVGLWLDGQKVS
                     QTDLSVPAQKEATVKFTLTVTRPGWHSGWVELPDDDLLADNRRFFAFYLPPQIRVVIV
                     SEAGQPAEHLRLALTAGDSSQSRFAVATIPPEKLLTYDLSPVNVLVFNELKKVPAGFG
                     ARLDSFQKHGGLFVILGTGFDLPSYQSAIFLRFGSVAVESGRDTIPGSGFYTLEQIDF
                     SHPIFQVYQKIPHEKFPQLRFFSVPKVTAGDLTRLASFSNGQPALVEKKAASQPALLF
                     AGPLNPTESDLPRHPFFVPFVQRTVEYLAFSPFAQAEQILVGDTLQRPVDFVYYGKKL
                     VVEDPAKQKLEASAFLTGEQAWVRIEPTDLPGIYQVKAGDDLAAAFAVNLDPRESNQP
                     AWEPASLKRQAPGLGVFTGDPSDPARAILSSRIGKELWKPLLWAAALLLMLEMLVARS
                     AAPREATEFPSS"
     gene            9498..9749
                     /locus_tag="AC16GM_10730"
     CDS             9498..9749
                     /locus_tag="AC16GM_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2924181.1"
                     /translation="MFIVYVIQSASGKRYTGHTSDLKHRLEEHNAGLCKSTKIDKEWL
                     VLLAEELANRSEAMKREKWLKAGAGRQFVQKVLAGWSPP"
BASE COUNT         2163 a         2930 c         2818 g         2026 t
ORIGIN      
        1 tgaagtaaag agcaaaatag ctgggccccg ccgtcggctg attgcggctg cggcggcgac
       61 gtatttcatc cgctcatctt tcggcgcgat agtaccgaaa gatttctctt gcatctcggg
      121 tgggacggtg tatattcttt ttaccctgcg gggctaccag ggatggtaaa agcgaaaagt
      181 gggttgtcaa ggggcaccag aataaagaag gatgaagcgg aaagagtttg acgtcattgc
      241 catcggtgct catccggacg atgtggaagt gggcacgggc ggggtcatga tcaagttgca
      301 ccaactgggc taccggaccg gtctcgtctc cctcacccgg ggcgaaatgg gcaccggcgg
      361 caccgcggct atccgcagaa aggaaacccg gcggggcgcc gccatcctgg gtgctcaact
      421 tttggccacg ttaaacttcg gggattgcaa actggtcgat tcttttacta accgactgaa
      481 ggtggcccgg cttttgcgaa agttccggcc ccgggtcgtc ttagctccct tttgggacgg
      541 cggccacggc aaacgtcagg gacatcccga ccacctggcc tgcggccgga ttgtcatgaa
      601 tgcggtcaac ttcgcgggac tgaaaaaaat gccgctgcgg gaagagccac accaggtgaa
      661 ggcgcttttc cattacttcc ttccggcggg gttgatgcct acgttcgtgg tggacatcac
      721 cgccgaattc ccgcggtggc gggaagccct gaaggcccac cgttcgcaat ttttgaatcc
      781 ggaaaaatct ctggattacc tctggttttt ggaatcaatg gcccgtacct acggcaatta
      841 tattcgtacc cggtacggtc aaggatttgt tattggagag ccgttcaaaa tcgacgacct
      901 caccctcctc gccggataac ctatgtcacc gagagccaaa aaaactgcgg cgaaaaccga
      961 tccaacgaca gtccggccct tgcctatgac gcccgaggaa ctccacctca gaggttggga
     1021 cagcctcgac attctattga tcacgggcga cgcctacatt gaccatcccg cggtccacgt
     1081 ggcactcatt gggcggctcc tggaatcgca tgggttcaaa gtcggtgtca tcgcccagcc
     1141 cgattggaaa aatctttccg atttctcccg tttgggccga ccgcgtctgg ccgtctacgt
     1201 caccggcggg cagaacgaac cgctcatcac ccattttttg gccaaagaca aaccgctttt
     1261 gcccgacgcc tggtcgccgg gtggcagtcc ggtcaagcgg ccggcccggg cggtgatgac
     1321 ctacgcgcaa agggcccgcc aggctttccc cggcttaccg gtcatcatcg gcggggtgga
     1381 agcttccctg cggcggctgg cttattttga cccctggaat aacgcggtgc ggcgctccat
     1441 tctcttcgat gccaaggccg accttttgat ttacacgcca ggagaatttg aaatgctggc
     1501 ggtggcccag cgtctggcgc aaggtgagcc gatcgagaaa atcaccaacc tccctggcac
     1561 tacagtgaaa cggagacggc tcccttcccg aggaccggta cgggaacttt tttcatacga
     1621 cgaagtgaaa accgacaaat tgaaatatct cgaggcgttc agcgatcagg cggtccagcg
     1681 ggcgcccgga gccggctttc ccgtagcgca gttgaccggc gaccggtacg tagtggtcaa
     1741 tccgccgcct gccagaacca ccgccgaact ggatctcctt tacgccttac ctttcacccg
     1801 cttgccgcat ccgtcgtacg gtgagcggga gaaaattgct gtggccgagg cactgcgctt
     1861 tgccattgtt agccatcgag gctgtgtttg cggcgcctct ttctgccagc acgaaccgta
     1921 ccagggcagc cagatccagt cacgcagtga acagtcaatt ctagatgaag cggtttcgtt
     1981 cgcctccgat ccggcctttc agggcatgat ttccgacttg agtggcccgt acgccaacca
     2041 ctacggcagt gatttcattt ccggaaacgg acagaaccaa tcccggaccg tattcgccga
     2101 gcaggggggg tcagacggcg atgggaacgg cggaggatcg actgcggcct tgaccgacct
     2161 gcgcgctgcg gaaatgccct tcggcgcaca acaggctttc acccttctct tgcagagatt
     2221 gcaaaaactt ccggggatca accgagtgct tttgacctca cccctccggc tggagtgggc
     2281 cgcggccgac cccgatttcc tgaaccaggt agccacccat cacgttcaga atcactgccg
     2341 gattatgccg ggccacgtca ccaaggccgt gaaccgtttg atgcggcggc ccggcgtgga
     2401 aatgctggat aaatttctgg ccctctttct ggacctctcg cagaaagcca aaaaagaaca
     2461 gtttatccag gcgcacttca tcatcaatca tcccggctgc cggacttttg atatggtcga
     2521 actggcggcc tacagccgca aaaggcgcct gaccctgaat tggttctggg atttctcacc
     2581 cgtaccgctc accgcctcgg gggtgatggc gtacaccgcg cttgaccccg agacccgcca
     2641 gcctttgtac gttccccgcg aggcgaagga ccggctgata cagaaagcct tagtgcgtta
     2701 cctggatccc ggcagccatt acctggtgaa gaaagcactc tatgcctgca accgggccga
     2761 cctcatcggc tccagttcgc tctgtctcat cagccccaag cccaaaccgc gggtgatgcg
     2821 caaaccggtg caggcacgcc cggatacaca tctccctccg cgtcccgccg ggacgaaccg
     2881 gctcgacgcc ttccggcacc ggtcgtactg agacagccgg taaacctttg tctcaccccc
     2941 tgtgtctaaa ataaaaggcg atctcgttga ggtatttttg ttctaccgcc gcggtaaaag
     3001 acaaaggata ctccttttga aacggagcgc cggttcaaac tggggcggcc tctggcaggg
     3061 ggtctcggga aagatcaaaa agggtgagtc ggccatccgg gcctgcttgc gggaaatcgg
     3121 agaagagacg aatttgagcg tgaaacgcct ctacgctctc gatctggtga accagttcta
     3181 tgcaccgcgc accgacactg tctacctcgg cccctttttc gccgccgaag tcaccacgcc
     3241 agaatctttg cgcctgagcc gggagcatga cgcctgccgc tgggctaccg tacaacaagc
     3301 gttgcgactg gtgatttggg aagggtaccg tcgggcgctg ctacgggtcc aaaacgatct
     3361 cttttcgaaa aatgcggtcg accggaactt cctcgtgtac gagcctcccg agagtaagca
     3421 gaagggatcg aaagcgcggc tgctgcgtat atagtaaaga gagcgaaccc aatcgatttc
     3481 gggagaacca acgtatccaa aagcgaaaag acagtgcaca attttcaggc gaggattgaa
     3541 tgaaaaaatg gatatggatt atcgtcgggg tcgtggccat tgccgccgtg gtcgtttatg
     3601 ccgccacccg gcagaacggc aaaaacgaca aaactcaatc agccacggga aaaattgtca
     3661 aaccgttccg gggcgacctg gtggtttcca tcaccgctac cggccagatc gagccgatag
     3721 atgccgtcga aattagatcg aaagcctcag gtgaaataat ccgcctgccc atcgacgacg
     3781 gcgtgtcggt caaaaagggc gacctgatcg cccaactgga ccagaccacc gcgcagaacg
     3841 actatgagca ggcgaaggcc gacttcggcg tggcggaggc gactttgaat cagcgcgaaa
     3901 aggaattgaa tcgggtgaag gggctgtttg agaaaaacct ggtctcgcag caggatctgg
     3961 ataatgccca actggcttat gagcaggcgc ggtcgcagtt cgtgcggacc aagacgacgc
     4021 tctccacgat ggaagagcgc ctgaaagaca cggtggtccg gtcgcccatc tctggggtcg
     4081 tcctgcagaa gaatgtcgaa gcaggacaga ttatctcctc ggcggtctcg tccgtttccg
     4141 gcggaacctt gattgccacc gtcgcgggta tggacaaagt ctacgtcgtg gccgacgtgg
     4201 atgagaccga catcggtaag gtggccatcg gccaaggagc caaagtcgtg gccgacgcct
     4261 accccgacga agagttccac ggccgcgtgc tcaaaatcgc accattggct aaagtcgccc
     4321 agaacgtaac caccattgac gtcaccgctg aggtagacaa cgctaaccac ttactcaaag
     4381 ccgggatgaa cgccaacgtt gacatcgtta ccgccgaagc ccggaacgcg cttttggtac
     4441 cggtggaggc gctggtggag ccggcggaag ccaaaatggc catggggaac ggtgctcccg
     4501 gcccgggtca atctgcagaa gcccaaacta aaccacccgc tgacgcgggc ggccaaacgc
     4561 gcgaaatttc accggaacgg cgcgagcgct tccgtcagat gatggagagc gggcaggtca
     4621 ccccggagat gatggcccag tggcagaaaa ggcgcgcggc gcgccgggat acctcggcgg
     4681 tgaacccgga aaagccgcac gcccgaacta aagtcgtcta cgttttggaa aaaggccagc
     4741 cgaccccgcg gaaggtgacg gtgggacttt cgaatctgga caatgcccag attgtcgacg
     4801 ggctcaccga acaggatgag atcgtcgtcc agtcctccag ccagttaatg cgcgaccgtg
     4861 aggaaatgaa acaaaggatg caaagattta cgggactgcc gggagtgaaa aaacagtagt
     4921 ccgggagctg gcataaacaa aaccaggatc aaagcaacgc aaatagacct atggccatag
     4981 cctggagtga agccctcggt gtctctttag gcgcccttcg cgccaaccgg atccgctcgt
     5041 ttttgaccat gctggggatt atcatcggcg tgggagcggt gatcaccatg atcgcgctgg
     5101 ggcaaggcgc gaagaaagca gtgcaggatc ggattgaatc cctgggggcc aatttgctct
     5161 tcgtccgtcc gggcgcgccc tttcgcggcg gggtgcgctt tgaatctggc tccaacgttc
     5221 gcctgaccga caaggatgtg gaggcgattc aaaccaaggt gcgcgggcta gaaattatgg
     5281 tccccgagac cggccgccgg gcgcaggtca agtacggcaa ccagaactgg aacacgcagg
     5341 ttagcggcac cattcccgag taccagttcg tgcgcaactc tccttgtatg gtgggaaatt
     5401 acttcacgaa aaacgacgtg gccacccgcc aacgcgtggc ggtcttagga catgaagtct
     5461 ggaacaacct gttcaacgga caagacccgg tgggcaagga agtccgcatc aataacattt
     5521 ccttcaccgt agtgggactg ttggctgaaa aagggcaggc gatgatgttc aaccaggacg
     5581 accagatttt gatccccatc accaccgcac aaaagcggct cttcggaatt gactttctga
     5641 ccaacgtcaa tgtgaaagtg gccgacgaag cggtgatgga acaggcggcc ttggacatcg
     5701 agcaggtgct gcgccgccag caccggctga agccgggcga ggagaacgac ttcaacatcc
     5761 gcagccaggc cgatatcctc tcgactttaa gcgaaacgtc ccaaaccttc accaccctcc
     5821 tcgcttcgat tgctttggtc tcgcttttgg tcggcgggat cgggattatg aacatcatgc
     5881 tcgtctcggt taccgaaagg acccgggaaa tcggaacgcg catcgccatc ggtgccaaaa
     5941 agcgggacat tatgaaccag tttttgattg aagcgctttt gctttccctc tgcggcggcg
     6001 ccatcggcgc ggcggccggc atcggcggct cggtgctgct atcgaaactg gccaactgga
     6061 acacgttgat ttcggtcagt tcgattttcc tggcgttcct cttttcggcg gcggtgggca
     6121 ttttcttcgg cctctacccc gcgcgcaaag ctgctcgcct gaacccgatt gaagcgctgc
     6181 gctacgagta gtaactccct ctttcagaat ccaaacgaga gattttccat tgaaccacgt
     6241 atattgtctc tatgcgtgcg ttctatgata gcgacgccga tctttcgctt ttgaaaggca
     6301 aaaccgtcgc cgtcatcggc tacggcagcc agggccgggc gcaggcgctg aatttgcgcg
     6361 acagcggcgc agcggttgtc gtgggactgc cgcagaccag cgcctcaaga aaaaatgcgc
     6421 gggaagatgg cctggcggtc tcttccactg aagaagccgg caagcgaggc gatttcattt
     6481 ctcttttggt tcccgatcaa ctgcaccaaa agatttttca aaagtcaatt cggccatatc
     6541 tgacgccgga caaaactttg gttatggcgc acggatacag cgtccacttc cggctgattc
     6601 aaccgcccac aaccgttgac acgcttctcg ttgccccttt aggtccggga gaaattctgc
     6661 gcgagcgttt cacccggggg aaaggcattg cctgtctttt ggcggtgcgc tcgaaaaacc
     6721 aaacctctgc ctggacgaaa gccaaaacct acgcccgggc cattggctgc acgcgtatgg
     6781 gagccattgc caccacgttc cgcgaggaag cggtgggcga tttattcggc gagcaggcgg
     6841 tactctgcgg agggttatct cagttacttt tggcggggtt tgaaacgctg gtgaaaaacg
     6901 gcatcaaccc ggagagcgca tacttagaat gtgtccagca gatcgacctc attatggcgt
     6961 tgatcaagcg gcacggctta cgcggcatgt tccagcgcat ctccgccacc gcggcttacg
     7021 gtgccgccag gagcggacgg aaagtgatag accaccgcgt gcggcgttcc atgcagcaaa
     7081 ttttcaccga tattgcttcc ggccggttcg cccgcaactg gcagaaaacg tttacggatc
     7141 gccataaagt tgaaaggctt ttggagacag cgtcggcgcc tgtttttgaa cgtgcccaca
     7201 agcgcgttca ggccctcctc cgcgagtaac cgatgtactt cctcaacccg ttcttcctct
     7261 tcgcgacgct cttcgctctt ttgccgctat taattcacct cttcaaccgg cagaaggtga
     7321 agaaaatcga gttcagttcg gtggctttcc tgaaagccat ggagcggacc aaaatccgcc
     7381 ggctcaaact gcggcagctc tggcttctcc tcctccgcac gctcatcatt ctcttagtgg
     7441 cgctcgcctt tgcccgtccg gcggtacggg ggaagttcgg gcggaaggtc ggctcgcacg
     7501 ccaaaacgtc ggcggtgatc atcctcgaca actccgccag tatgggcttg gagaccaaga
     7561 atggttctct gttcgaattg gcgcagaaaa agacacagaa gatcatcgac ctgttcgccg
     7621 aaggggacga aacggcgctc gttttgacca atggcggcgg ccagccgacg ctgacgcata
     7681 acttccgccg gctctcccgc gacctggcgg aggcccaact ttcctccggc gccaccgccc
     7741 tcgaccgtac catcgcttcc gccgcccgac tcctttcatc ttcgaaaaat ttgaaccgcg
     7801 aaattttcct cttctctgat ctgaaaagtc ccgccggagc aatttccgac accgccttgt
     7861 cccatttcca cggcaaactg tttctcttcc ccgtcaaagc tgctgattac gaaaacgtaa
     7921 gtctccagga aattgatttt ggccgccagt tgatcaccct gggcgtgccc tttcagatga
     7981 ccgccaaagt ccaaaaccag agtccgcgcc cggaggaagg tctcttggtc ggtctctggc
     8041 tcgacggaca gaaggtgagc cagaccgatc tctcggtgcc ggcgcaaaag gaggcgacgg
     8101 tcaagttcac gctgaccgtc acccgtcccg gctggcacag cgggtgggtg gaactgcccg
     8161 atgacgacct cttggccgac aaccggcgct tctttgcctt ctatctgcca ccgcaaatcc
     8221 gggtggtgat tgtttccgag gcgggccagc ccgccgaaca cctgcgcctg gccctcaccg
     8281 cgggagattc cagccagagc cgttttgccg tggccaccat ccccccggag aagttattga
     8341 cctacgacct cagccccgtg aacgttttag tcttcaatga actgaaaaaa gtgcctgccg
     8401 gatttggtgc gcggcttgat tccttccaga agcacggtgg acttttcgtc atcttgggga
     8461 cggggttcga tctgccgtcc taccagagtg ctattttttt aaggtttggc agcgtcgcag
     8521 tagagtcggg ccgggatacc atccccggtt cgggtttcta caccctggag cagattgact
     8581 tttcccaccc aatctttcaa gtttatcaga agatccccca cgagaaattt ccgcaactcc
     8641 gctttttcag cgtgcccaaa gttaccgccg gcgacctgac ccgtctggcc agtttcagca
     8701 acggtcagcc cgccctggtg gaaaagaaag cggcaagcca gccggctctt cttttcgccg
     8761 gccccttgaa cccgacggaa agcgatctcc cccggcaccc cttctttgtc ccctttgtac
     8821 agcgcaccgt tgagtacctg gctttctccc ccttcgcgca agcggaacag attctggtgg
     8881 gggacacgct ccagcgcccg gtcgattttg tttattatgg caagaaactg gtagtggaag
     8941 atcccgccaa gcaaaaactc gaagcgagcg ccttcctcac cggcgaacag gcctgggtgc
     9001 ggattgagcc aacggatcta cccgggattt atcaagtgaa agcgggtgac gatctggcgg
     9061 cggcctttgc ggtcaacctc gatccccggg agtcaaacca accggcctgg gagccggcct
     9121 ctctaaagcg tcaggcaccc ggactgggcg tcttcacggg tgatccgtcc gaccccgccc
     9181 gcgccattct gtccagccgc atcgggaaag aactctggaa accgctgctc tgggccgcgg
     9241 cgctgctcct gatgctcgaa atgctggtag cccgcagcgc cgctccgcgg gaagcaactg
     9301 aatttccttc aagctgacaa acgttccgcc ggggctgtgt gactggactc cttgacgccg
     9361 ccaggaaaag atgctatcat gccctcgacg cggggtggag tccgccaccc gccgcggcgg
     9421 ggagtagctc gtcgggctca taatcccgcc acaggcggat tcaaatcgcg cccccgctat
     9481 ttttttagca cttcttcatg ttcatcgttt acgttattca gtcggcttcc ggtaaacgtt
     9541 ataccgggca tacctctgat ctgaaacacc gactggaaga acacaacgcc ggactttgta
     9601 aaagcaccaa aatcgataag gaatggctgg ttcttttagc cgaagagtta gccaatcgtt
     9661 ctgaagcaat gaaacgagaa aagtggctga aggcgggggc tggtcgtcag tttgtccaaa
     9721 aagtactcgc ggggtggagt ccgccatagg cggagtagct cgtcgggctc ataatccgcc
     9781 tcaggcagat tcgggtctgt cgacgaatcc acgacacgct tacaaacgtt tcaccgagat
     9841 tacgtggacg ccttgactca gccgagaaaa gatcgtatct tgcctttgac gcggggtgga
     9901 gcagtccggt agctcgtcgg gctcataacc cgaaggt
//
LOCUS       DBNGZD010000413         5993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1662191, whole genome shotgun sequence.
ACCESSION   DBNGZD010000413 DBNGZD010000000
VERSION     DBNGZD010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1662191"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="AC16GM_10735"
     CDS             complement(<1..1008)
                     /locus_tag="AC16GM_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924182.1"
                     /translation="MRQNFLRALFWGSLLVLTRGAVAPAADFSGTLATSVYSFERITS
                     TEEIPQSRGNQTFTLFANDLTSGRGGLLSGHMFLRFRTDWQNALPGDPQWNFYNLYFD
                     YRTKSQKLAARVGRQFVTAGWGATSLDGAKISVRPIRNITVQAFGGSAIDPLNPQKVR
                     PLARNEIWGGYLAWDWRFKAHLGFSLFQKNFERSKDRYLIGADGRYDFGRVSFLARAN
                     YDYDRTSALFQRFFPLRLKKLQLLSLESRQTINPQFTTGLEYFFHRPLFSARSFMTRF
                     SQSTYHDWRTSGNYFLTPKIELQGEMGWALLEKEEGAPGSDNAFRLRLGVAASDFAFG
                     YV"
     gene            complement(1435..1752)
                     /locus_tag="AC16GM_10740"
     CDS             complement(1435..1752)
                     /locus_tag="AC16GM_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR2924183.1"
                     /translation="MDGAEVLAKVAHLPISTWNYKSQPTSIRHIGPMAQDFYALFGVG
                     EDDKIISTIDPPGIALAAIQELYRENETLNARVSELESLVQTLLAGRQDKAAGDYHFG
                     SAR"
     gene            complement(1756..2685)
                     /locus_tag="AC16GM_10745"
     CDS             complement(1756..2685)
                     /locus_tag="AC16GM_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924184.1"
                     /translation="MAGVVRPGSPQAAVPQLINYQGKLTNASGQPITSAVTLKFRIYD
                     TPTGGTLLWGPESQPVTPDANGVYNVILGQGTPPVPFPANLFAATPRYIGVEVNSEGE
                     MTPRQQITAVAYSLRVATVDGATGGTISGDVSIQSDLSVSGKATIGPNGNSGGFAFVA
                     GASNNVTNIGATVGGGQSNNASRDFATIDGGIGNNASGENSTVGGGRFNTAAGSNSTV
                     PGGASNRADGDYSFAAGRRARAFHTGTFVWADSNDFQFASEGPNQFSARTTGGARFVT
                     ATDGAGNPTAGVFLFGGANSWSSISDRNAKTNF"
     gene            complement(3076..3858)
                     /gene="lepB"
                     /locus_tag="AC16GM_10750"
     CDS             complement(3076..3858)
                     /gene="lepB"
                     /locus_tag="AC16GM_10750"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR2924185.1"
                     /translation="MNGKKDQAKPSASGGKLKKSFDQVLWEYVKSLAIALILALGIKT
                     SLVEAYKIPSGSMEDTLLVGDFLLANKFLYGSKIPLVNAHLPAIRDPKPGDVVIFKYP
                     VDRKTNYIKRCIAVGGQTVEIRNKKVYVDGKEYPFPAHGKFTDTMIMPQAGGGAAPFN
                     PYDPHNPLNRDNFGPYRVPEGMFFMMGDNRDNSFDSRFWGPVPRNLILGKAMVIHWSW
                     RRDERAPVWSWTNPFSMAKAFGYNLVHFFQRVRWNRLGSLVR"
     gene            complement(3992..5053)
                     /gene="rseP"
                     /locus_tag="AC16GM_10755"
     CDS             complement(3992..5053)
                     /gene="rseP"
                     /locus_tag="AC16GM_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR2924186.1"
                     /translation="MTTLLATIFVLGLLVFVHELGHFLVAKRAGIKVERFSLGFPPKM
                     IGKKIGETEYCISWLPLGGYVKMAGEEPDAAQVTGAPYEFMSKPIWIRSLVVVAGPVM
                     NYLLAVLLIWGIFFLGGRTEVDENQIVVGTVMPGSPAEKAGIKPGDKIMAVDGQAVKT
                     FDEMREKIYAKVEKPVEVTWLRDGREMRATIVTFKDKSRNEKGQPVAVGRIGIGQAFR
                     LYKVGFFEAARLGVVTSWDMAALVGQFLYRLIIGQESFKSLGGPVFIAQIAGESAHRG
                     FADLLMFAALLSVNLALLNILPIPALDGGHLLFLFIEKLRGKPLSLKQRAVIQQVGFA
                     FLILLIVFATYNDILRIFH"
     gene            complement(5050..>5993)
                     /gene="dxr"
                     /locus_tag="AC16GM_10760"
     CDS             complement(5050..>5993)
                     /gene="dxr"
                     /locus_tag="AC16GM_10760"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR2924187.1"
                     /translation="LVGEEGIVELAQMPEADLVLNAIVGSAGLLPSLAVLAAGKTLAL
                     ANKESLVMAGELLMVQARVNNVEIFPIDSEHSAIKQCLFAGRHPEVRRIILTASGGPF
                     REKSRDEFAQVTVNQALAHPTWNMGKKVTIDSATLMNKGLEIIEAHHLFGMPQEKIEV
                     AIHPQSLVHSLVEFVDGSVLAQISPPDMKIPLQYALLYPERLSSENGLLDLTRTGTLE
                     FSEPDLTKFPSLLLARRALTLGGTAPAVLNAADEVAVQAFLDEKIGFGEIPQVVRAVL
                     DEHTVIDDPTLDDILKADRWAKSKAGEFVGITEEAAR"
BASE COUNT         1319 a         1741 c         1671 g         1262 t
ORIGIN      
        1 gacgtagccg aaggcgaaat cggaagccgc cacccccagg cgcagccgga aggcattgtc
       61 gctgccgggg gcaccttcct ctttttcgag cagcgcccag cccatttcac cctgaagttc
      121 aattttcggt gtcaaaaagt aattgccgct ggtgcgccag tcgtggtagg tgctctggga
      181 gaagcgggtc ataaacgagc gggcggaaaa gagaggccgg tggaagaagt attccaagcc
      241 ggtggtgaat tgggggttga tggtctgccg gctttccagc gagagcaatt ggagtttctt
      301 caggcgcagc ggaaagaagc gctgaaagag cgcgctcgtc cggtcgtaat cgtagttcgc
      361 ccgcgccaga aaactgaccc ggccaaagtc atagcgtccg tcggcaccga tgaggtagcg
      421 atcttttgac cgctcgaaat ttttctggaa aagcgagaaa ccgaggtgcg ctttgaagcg
      481 ccagtcccac gccaggtacc cgccccagat ttcgttgcgc gccagcggcc gcaccttttg
      541 agggttgagc gggtcgatgg ccgagccacc gaacgcctgc acggtaatgt ttcgaatcgg
      601 tcgaacgctg attttggccc cgtctaagga cgtggctccc cagcctgcag tgacaaactg
      661 tcggcccacc cgcgctgcga gtttctgcga cttcgtccgg taatcgaagt agagattgta
      721 gaagttccac tggggatccc ccggtaaggc gttctgccag tcggtgcgga agcgcaaaaa
      781 catatggccg gacaatagcc cgccccggcc ggaggttaaa tcattggcga aaagggtgaa
      841 tgtctgatta ccacgcgatt ggggaatttc ttctgtagaa gtaatccgct cgaatgagta
      901 gaccgaggtg gcgagcgttc cggaaaagtc ggcggcgggg gcgactgccc cacgggtgag
      961 gacgagcaaa cttccccaga aaagagcgcg caaaaaattc tgtcgcatct gttggtgcaa
     1021 ccgtgttcga tacgaactcg taccgagcca agctacgtcc ggttcccgtg agtgtcaaga
     1081 aaaacgaagg ggtggacatt tacctccacc cgaatgaatc tcagacttcg gtcaggcagg
     1141 atgcctgacc tatggaaggg aaggtgtttt tacttccata gccgaggctt tcagcctcgg
     1201 aaggcactct ggccctacag cccaggggct tttccccgta acctggtaga ccggctcgtc
     1261 ctcgatagag tcgcaccgac ccgagctggt caaaaaataa aagtcggact tcagagacga
     1321 gcccgactac cagtattgcg tgtcaagaaa attgtagggg cggacgttta cgtccgcccg
     1381 aacagttctt ttcgggcgaa cctaaaggta cgcccctacg ccactgtcgt caggtcaccg
     1441 ggccgaacca aaatggtaat caccagcggc cttgtcctgc cttccagcca agagtgtctg
     1501 aacgagactc tccaattcgc tcacccgcgc gttgagcgtc tcattctccc tgtaaagttc
     1561 ctgaatggcg gccagggcaa tccccggcgg gtcgatggtg gaaataatct tatcatcttc
     1621 acccacgccg aacagcgcgt agaaatcctg cgccatcgga ccgatgtgac ggatggaggt
     1681 gggctgtgat ttgtaattcc aagtactgat aggcaagtgg gccacctttg ccaaaacctc
     1741 cgccccatcc actggctaga aattcgtttt tgcgtttcga tcgctaatgg acgaccaaga
     1801 gttagcacca ccgaatagaa aaactccagc tgttgggttg ccggctccgt cagtagctgt
     1861 tacaaatcga gcgccaccgg tagtgcgagc gctaaattga ttcgggcctt cagatgcaaa
     1921 ttgaaaatcg ttactgtcgg cccacacgaa ggtaccggtg tggaacgctc gcgcacgccg
     1981 tccagccgca aaagagtaat cgccgtctgc tctgtttgaa gcgccaccag gaactgttga
     2041 attacttccg gcagcggtgt tgaagcgtcc tccgccaact gttgaattct ctccggatgc
     2101 gttgttgcca attccaccat ctattgtcgc gaagtctcta gatgcgttat tgctttggcc
     2161 acctccgaca gttgctccaa tattggtgac gttgttactc gccccagcta caaaagcgaa
     2221 accgccgctg ttgccattag ggccaatagt ggctttaccg gagacagaga ggtcgctctg
     2281 gatgctcaca tcgccggaaa tcgttccgcc ggtcgcgcca tcgacggtgg caactcgtaa
     2341 tgagtaggcc acggcggtga tctgctggcg gggagtcatt tccccctcgg agttcacctc
     2401 cacgccaatg tagcgtggag tggccgcgaa caagttggcg ggaaagggca ctggcggcgt
     2461 cccctgcccg agaatgacgt tgtagacgcc gttggcatcg ggtgtcaccg gctgcgattc
     2521 ggggccccac aagagtgtcc cgcccgtcgg agtgtcgtag atgcggaact tcaacgtcac
     2581 cgccgaggta atcggctgcc cggaagcgtt ggtcagtttc ccctggtagt tgatgagttg
     2641 cggcaccgca gcttgtggtg agccaggtcg aaccacgccg gccattgttg ccacaaggca
     2701 cagcgccgtt gtcaagagca aagttcgcat gcgcacggta aacctccttc tggagttgct
     2761 tgcgacccag tggtttggaa aatgtgaacg gtgtgaaacg aatcgcaaca tgaattcccc
     2821 attacgacac ctcgcggtca ccggttcatc acctactaac agaacgcccc ccctcgctga
     2881 aaaagatatg gggtgaactg aacctgtcaa ggatttttag agttttttag gagttatatg
     2941 gtcttgtatc gcctgggcat tgaagagttg ttctccgtgc ctcctcaccg ttggggatct
     3001 tcaactgcgg tacgcattct ctacgcaaaa taaaaacccc gcttttggcg gggctgtctg
     3061 cagagagaaa atcgcttagc gaaccagcga ccccagccgg ttccagcgca cccgctggaa
     3121 gaaatggacg aggttgtagc cgaaggcctt ggccattgaa aacggattgg tccacgacca
     3181 gacgggcgcc cgttcatccc gccgccacga ccagtggatc accatcgcct tgcctaaaat
     3241 caagttgcgc ggcaccggcc cccaaaagcg ggagtcaaac gagttgtccc ggttgtcccc
     3301 catcataaag aacatccctt ccggcacccg gtaggggccg aagttgtccc ggttgagcgg
     3361 gttgtgcgga tcgtaggggt tgaagggcgc cgccccaccg cccgcctgcg gcataatcat
     3421 ggtatcggtg aacttgccgt gcgccgggaa cgggtactcc ttgccgtcaa cgtacacttt
     3481 cttatttcgg atctccaccg tctggccccc caccgcaatg cagcgcttga tgtagttggt
     3541 cttccggtcc accgggtact tgaaaatcac cacatccccc ggcttggggt cgcggatggc
     3601 cggcaggtgg gcgttgacca gaggaatctt cgagccgtag aggaatttgt tcgccagaag
     3661 gaagtcgccg actaaaagcg tgtcttccat tgagccggag ggaatcttgt acgcctccac
     3721 cagcgaggtt ttgatcccca gggctaagat taaggcaatg gctaacgact ttacgtattc
     3781 ccacaggacc tgatcgaacg atttctttag ttttccgcct gaggcggacg gtttagcctg
     3841 gtctttcttc ccgttcattt accctcccaa agcatctttc atttacactg gtatacgtaa
     3901 ggaagccgtc tctgaactaa taatacgtga accggaacct gtcaatcgcc aaaaggaaat
     3961 gccgtttctc cggcggaaac cgtcatccgg ttcagtggaa gatgcgcagg atgtcgttgt
     4021 aagtggcaaa gacgatcaaa aggattaaaa aggcaaaacc cacctgctgg atgaccgccc
     4081 gctgcttgag cgagagcggc ttgccgcgca acttctcgat gaataagaag aggagatgcc
     4141 cgccgtccaa ggccggaatg ggcagaatgt tcaaaagcgc taagttgacc gacaggagcg
     4201 cggcgaacat caaaaggtcg gcgaagccgc ggtgggccga ttccccggcg atctgggcga
     4261 tgaagaccgg cccgcctaaa gatttgaacg actcctggcc gatgatgagc cggtacaaaa
     4321 actgccccac cagagccgcc atatcccaac tggtgaccac ccccagccgc gccgcctcga
     4381 agaagccgac tttgtacagg cggaaggcct gaccgatccc gatccgcccg acggctaccg
     4441 gctgaccttt ttcgttgcgc gatttgtctt tgaaagtgac gatggttgcg cgcatttccc
     4501 gcccgtcccg cagccaggtg acttcaaccg gtttttccac tttggcgtaa atcttctccc
     4561 gcatttcgtc gaaagttttg accgcctggc cgtccacggc cataatcttg tccccgggct
     4621 tgatccccgc cttttcagcg ggactcccgg gcataaccgt cccgacgacg atctggtttt
     4681 catcgacttc agtccggccg ccaaggaaaa agatgcccca gattaaaagg accgccaaga
     4741 ggtaattcat caccggcccg gcgacgacca cgagtgaccg gatccagatc ggcttggaca
     4801 taaactcgta cggcgcgccg gtgacctgag ccgcatccgg ctcctccccc gccattttga
     4861 cgtacccgcc cagaggcagc caggagatgc agtattcggt ctccccgatt ttcttgccga
     4921 tcattttcgg ggggaaaccc aaagaaaaac gttcgacttt gatcccggcc cgcttggcca
     4981 ccaaaaagtg gcccagttcg tggacgaaaa ccagaagccc caagacgaat atggttgcca
     5041 gtagtgtggt caccgcgctg cctcttccgt tatgccgacg aactctcctg ctttgctctt
     5101 ggcccaccgg tcggctttca gtatatcgtc caatgtaggg tcgtcgatga ccgtgtgctc
     5161 atccagcacc gcccggacaa cctgcgggat ttcgccaaaa ccgatttttt cgtccagaaa
     5221 ggcctgcacc gcgacttcat cagccgcatt gagcaccgcc ggcgcggtcc cgcccagagt
     5281 caaagccctg cgggccaaaa gcaaactggg aaactttgtc agatcgggtt cgctgaattc
     5341 cagcgtgccc gtccgggtca aatccaacag cccgttttcc gacgacaacc gctccggata
     5401 aagcagcgcg tactggagcg ggatcttcat atcgggcggg ctgatctgtg ccaaaaccga
     5461 gccgtccacg aattccacta aagagtggac gagcgactgc gggtgaatgg ccacttcgat
     5521 tttctcctgc ggcatgccga acaggtggtg cgcctcgata atctccagcc ctttgttcat
     5581 caaggtggcc gaatcgatgg tcaccttctt ccccatattc caggtggggt gcgccagggc
     5641 ctggtttaca gtgacctgag cgaactcgtc tctggatttc tcccggaacg gcccgcccga
     5701 ggcagtgagg atgatccgcc gcacttccgg atgccttccg gcgaaaagac actgcttgat
     5761 ggccgagtgc tcgctatcga tgggaaaaat ttcgacgtta ttgacccgcg cttggaccat
     5821 caagagttcc ccggccatta cgagcgattc cttgttggcc aaagcgagcg ttttgccagc
     5881 agcaagaacg gctaagctag gcaaaagccc cgccgagccg acgatggcgt tcaataccag
     5941 atcggcttcg ggcatctgcg ctaattcgac gatcccctct tccccgacca aca
//
LOCUS       DBNGZD010000414         5937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_552386, whole genome shotgun sequence.
ACCESSION   DBNGZD010000414 DBNGZD010000000
VERSION     DBNGZD010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_552386"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..714
                     /locus_tag="AC16GM_10765"
     CDS             187..714
                     /locus_tag="AC16GM_10765"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HIR2924188.1"
                     /translation="MKGKIVVVPMGDFDFYLVNKLATALSTMFAVPVDILQGMKIPQE
                     AYNWERGQYYSTVILSKLELLKANERERVLGVVDEDLYVPALNYVFGESDRLASVAVI
                     SLYRLRQDLFTAGDDDRLVYQRMLKEGVHELGHVYGLSHCNNLKCVMYFSNSMLDTDR
                     KSEKFCDVCLRRLSN"
     gene            complement(704..1678)
                     /locus_tag="AC16GM_10770"
     CDS             complement(704..1678)
                     /locus_tag="AC16GM_10770"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR2924189.1"
                     /translation="MTRLIKAHGLLEKQPGFYTRNILLTWGLLAVGLFFLAVTDNFWL
                     LLLDAAYLAFVFTQISFLSHDAGHRQIFRAAWQNDIFCLVHTDLLLGMSYGWWVKKHN
                     QHHNAPNQVDHDPDLDIVALALSEDQIQSKHGFLRFVAKYQAYFFFPLLLLTAFAMSA
                     KSVQAVLQKKVKYPRVEALLISLHFALWIGLLCYWLGIRQALLFTLLQRALFGLYLGS
                     VFAPNHKGMLILKENNRLDFLRQQVLTTRNVKAHPVTDFWYGALNYQIEHHLFPSMAR
                     NKLSGAQKIVRTFCREHAIPYYESRVLQSYREILQYLHQVSASVSSNY"
     gene            2028..4325
                     /locus_tag="AC16GM_10775"
     CDS             2028..4325
                     /locus_tag="AC16GM_10775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2924190.1"
                     /translation="MASETDILTGKPTLADLLGQLKSDPQFARNIVHWKVLPALPGQY
                     ADFPVRVDQRLRSVLEKRGISRLYSHQAEAMEKIFSGKHMVIVTPPASGKTLCYNLPV
                     LQSILNDPGTRALYLFPTKALSQDQLAELTDLVNAANADIKTYTFDGDTPQDARRAIR
                     QAGHIVVTNPDMLHAGILPHHTKWIKLFENLKYVVIDELHNYRGIFGSHLGNVIRRFK
                     RVCAFYGSKPQFVFCSATIGNPVELAEKILEDKVELVDNNGAPRGEKHFILYNPPVVN
                     AQLGIRRSAVSEAQRLAQRFIGHDVQTIVFAPYRLTVEVLLTGLQEQLRKQGKPDDLV
                     RGYRGGYLPLERREIERGLRDGSIKGVVATNALELGIDIGQLEVAILTGYPGNIASTL
                     QQAGRAGRRQGISVAILVATSSPLDQFLMQKPEYLFSRSPETGNVDPNNLVILTSQVK
                     CAAFELPFEEKEKFGLDATVRLLDHLVEHQVLHKTDGRYHWASEIYPANEVSLRSATP
                     EDFVILNRSDNNRVIGEVDYASAPIFLFPEAIYLHGGEKYQVEELDWEGKKAYVKEAP
                     VDYYTDAETKTELKVLRVANESERSGGQLAHGEVAVTTVPVLFKKIKFFTHENVGNGK
                     ISLPEQTMHTTAFWYVFPENVATITNIPKAQLGSSLRGLANLLQGLVPFWLLCDRRDI
                     RVVPQVKAPFTEKPTLYIYDNVPGGVGFAERLFRIFPGVIQPCFDFLRACGCESGCPA
                     CVGPMLEAEQSGKAGVEKLLLFLKG"
     gene            4668..4961
                     /locus_tag="AC16GM_10780"
     CDS             4668..4961
                     /locus_tag="AC16GM_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR2924191.1"
                     /translation="MIILSAIFSRRTKSLKKFTPYECGVDPVGSARERFPIKYYMVAM
                     LFIIFDIETVFLYPWAVVYKQLGWFGFIEMGIFILLLFLGYVYVLKKGALRWD"
     gene            4979..5506
                     /locus_tag="AC16GM_10785"
     CDS             4979..5506
                     /locus_tag="AC16GM_10785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR2924192.1"
                     /translation="MEALQEELKDQVALTTVEKLVNWARKGSLWPLGFGLACCAFEMM
                     SAQASKFDLSRYGMELMRPSPRQADLMIVTGRVSTKMAPVVRQLYDQMPNPKWVLSMG
                     ACASTGGIFNNYAIVQGVDKIVPVDVYAPGCPPRPEQLINAIMVLREKIAREKISGQK
                     EKPIVIGWQQVPVKT"
     gene            5584..>5937
                     /locus_tag="AC16GM_10790"
     CDS             5584..>5937
                     /locus_tag="AC16GM_10790"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR2924193.1"
                     /translation="MTEPKYVTKLKEKFPGDFVEVNEFRGEWTAVIRTESIVDLCRFL
                     RDDPDCQFNFLSDLCGVDRLNLNQTPRFEVVYHLFSLKFYHRLCLKVSIEETRAVPSV
                     TGVWSAANRHEREVFD"
BASE COUNT         1335 a         1576 c         1731 g         1295 t
ORIGIN      
        1 gggaactgcg ccggagggcg tttgtttcgg ccacgcgcgg ccttcaggta aaacgagcgc
       61 agctgggcaa ccgggccggc ttcatcggcg ccgctttgta cgtcggcgaa gcgcaaaatc
      121 gttgacagcc ctacctcccc cggttatgct ttcgtttgat agcgactatt cgaggcacgc
      181 tcacgcatga aaggcaaaat cgtcgtcgtt ccgatggggg atttcgactt ctacctcgtc
      241 aacaagctgg cgacggcgct ctccaccatg ttcgcggtgc cggtggacat cctgcagggg
      301 atgaaaattc ctcaagaggc ctacaactgg gagcgggggc agtactactc gaccgtcatt
      361 ttgagcaaac tcgaactgct caaagccaac gagcgggaga gagtcctggg cgtggtggat
      421 gaagacctct acgtcccggc gctcaactac gttttcggcg agtcggaccg cttggcgtcg
      481 gtggcggtga tttcgctcta ccgcctgcgg caggacctct ttaccgccgg cgacgacgac
      541 cgcttggtct accagcggat gctcaaggaa ggggtgcacg aactgggcca cgtctacgga
      601 ctctcgcact gcaacaacct caagtgcgtg atgtacttct ccaactcgat gctcgacacc
      661 gaccggaagt cggagaaatt ctgcgacgtt tgcctgcgca ggctcagtaa ttagaagaaa
      721 ccgaagcgct cacttggtgc agatattgca ggatttcacg gtacgactgc agcacccggg
      781 attcgtaata gggtatggca tgctcccggc agaaggtcct gactattttc tgcgccccgc
      841 tcaatttgtt ccgggccata ctcgggaaca ggtggtgctc gatctggtaa ttgagtgcgc
      901 cgtaccagaa atcggtgacc ggatgggctt ttacgtttcg cgtcgtgaga acctgctgcc
      961 gcaggaagtc cagccggtta ttctccttca ggatgagcat gcctttgtgg ttgggggcga
     1021 agaccgagcc cagatacagc ccgaaaagcg cccgctgcag gagcgtgaac agcagcgctt
     1081 gccggatccc cagccagtag cagagcagcc cgatccaaag tgcaaagtgg aggctgatga
     1141 ggagcgcttc gacccggggg tactttactt tcttctgcaa aacggcttgc acactcttgg
     1201 cactcatggc gaaggccgtt aacaacagca ggggaaagaa aaaataggcc tgatatttgg
     1261 caacgaatct tagaaaaccg tgcttgctct ggatctggtc ttcggagaga gccagagcaa
     1321 cgatgtcgag atcgggatca tgatcgacct ggttgggagc gttatggtgc tgattgtgtt
     1381 tcttgaccca ccagccgtag ctcattccga gtaacaggtc ggtgtggacg aggcagaaaa
     1441 tatcgttctg ccaggcagcg cggaaaatct gacggtgacc ggcgtcgtgg ctcagaaagc
     1501 ttatctgcgt gaagacaaag gcaaggtaag cggcgtcgag cagtagcagc cagaagttgt
     1561 cggtgaccgc gaggaagaag agccccacag ccagcaggcc ccacgtgagc aagatattgc
     1621 gggtatagaa gcccggctgt ttctccagta gcccatgcgc tttgatgagc cgggtcaatt
     1681 ctccatattc atttgtcggt gagtgttgtg gcgacaccgg ggaataaaga tgaggccttt
     1741 taccgcccgt tacctcaact ccacgatctc tactcagcaa cgacgcctga ttcttttttt
     1801 tgtttaagca agtcagcctc cgtgtcgatt cgctccgtga cttcaaagcg agaattccat
     1861 ttctccttcc atcacttttt ttcacatcaa aggtgtcttg ttcaaaaaat aggggacgtc
     1921 attgccctca gtcaagggaa aaattacgat tgcaatttga cggtttgggt attttcttgt
     1981 cggcacgcta agttaagact gttcttaagg cggaccaggt tcatactatg gcatccgaaa
     2041 ccgacatcct gaccggaaaa ccgactctgg ctgacctgct cgggcaactg aaatccgacc
     2101 cgcagttcgc caggaacatc gtccactgga aggtcttgcc cgcgctgccc ggccagtatg
     2161 ccgatttccc ggtgcgggtg gatcagcgtt tgcgttcggt tctggaaaaa cgcgggatct
     2221 cgcggctgta ttctcatcaa gccgaggcga tggagaaaat tttttccggc aaacacatgg
     2281 tgatcgtcac cccgcccgct tcgggcaaaa cgctctgcta caatttaccg gttctgcagt
     2341 ctatcctgaa cgatcccggc acgcgggcgc tctatctttt tcccaccaag gcgctgtcgc
     2401 aggatcagct ggccgagtta accgatttgg tcaacgcggc gaatgccgac atcaaaacct
     2461 acacctttga cggcgacacg ccgcaggatg cacggcgggc gatccggcag gcggggcaca
     2521 tagtggtcac gaacccggat atgctccacg caggcattct gccgcaccat accaagtgga
     2581 tcaaattgtt cgagaatctc aagtacgtcg tcatcgacga actgcataac tatcgcggca
     2641 tcttcggcag ccacttgggg aacgtgatcc gccggttcaa gcgggtttgc gccttctacg
     2701 gttcaaagcc gcagtttgtc ttctgctcgg ccaccatcgg caatccggtg gagttagcgg
     2761 aaaaaattct ggaagacaaa gtcgaattag ttgataacaa tggcgcgccg cgcggggaaa
     2821 agcatttcat tctctacaat ccgccggtgg tgaacgcgca gttggggatt cgccgttcgg
     2881 cggtgagtga ggcgcagcgg ctggcgcagc gctttatcgg ccacgacgtt caaaccatcg
     2941 tttttgcgcc ataccggctc acagtggaag tcctgctcac cggcctgcag gagcagttgc
     3001 ggaagcaggg caaaccggat gacctcgtgc gcgggtatcg cggcggctac ctccctcttg
     3061 agcgcaggga gatagagcgc ggcttgcgcg acggttccat taaaggggtt gtggcaacta
     3121 atgcgttaga attagggatt gacatcgggc agttggaagt cgccattctc acgggctacc
     3181 ccggcaacat cgcctcgacc ctgcagcagg cggggcgggc gggacgcaga cagggaatct
     3241 ccgtcgcgat tttagtcgcc accagttcgc ccttagatca gttcctgatg caaaaaccgg
     3301 agtacctttt ctcccggtcg cccgagacgg ggaacgtcga tccgaataac ctcgtcatcc
     3361 tgacgagcca ggtgaagtgc gcggcgttcg agctgccgtt cgaagagaaa gaaaaattcg
     3421 gtttagacgc cacggttcgt ctgctcgacc atttggtgga gcatcaggtg ctgcacaaga
     3481 ccgacggccg ctaccactgg gcgtcggaga tttacccggc caatgaagtc tccctgcggt
     3541 cggctacgcc ggaggatttc gtcatcctga accgctcgga taacaaccgc gtcatcggcg
     3601 aggtcgatta cgcaagcgcg ccgattttcc tctttcccga agcgatctac ctgcacggcg
     3661 gggagaagta ccaggtggag gaattggact gggaagggaa gaaggcgtac gtgaaagaag
     3721 cgccggtgga ttactacacc gatgcggaga ccaagactga actgaaagtc ctgcgggtgg
     3781 cgaacgagag cgaacgctct ggcgggcaac tggctcatgg cgaagttgcg gtaactaccg
     3841 ttccggtact attcaaaaag atcaaatttt tcactcatga aaacgtgggc aacgggaaaa
     3901 tttctttgcc agagcagacg atgcacacca ccgctttctg gtacgtcttc ccggagaacg
     3961 tcgctaccat caccaatatt ccgaaagcgc agttaggttc cagtctacgc gggctggcca
     4021 acctgctcca ggggttggtg ccgttctggc ttttgtgcga tcggcgggat attcgcgttg
     4081 tcccgcaggt gaaagcgccg ttcaccgaaa aaccgacgct ctacatctac gacaacgtac
     4141 ccggaggggt cggtttcgcc gaacggctct tccgcatttt ccccggagtg atccaaccct
     4201 gtttcgattt cttgcgcgcc tgtggctgtg aaagcggctg tcccgcctgc gtggggccaa
     4261 tgctcgaagc agagcaatcg ggaaaggcgg gagtggagaa gttgctttta ttcctcaagg
     4321 gataaaggat tgttgagaac ctgctttccg aggctaaaag cctcggctat ggggtggaag
     4381 gcgctttcgg tccataggtc agaccgaagc cataacattt gaacgtgaac gctaagccat
     4441 gaaaatgatc agggggaaag aaacgacata aatagccgac tgttcttgtc aagaattacg
     4501 ggttgacaga acagattgaa aattcgtatt atattcaaag agtacaagat gtttttggta
     4561 tgaaagaatt tgtgaaaata ttcacataga tgacgtatca ggggtaaatg aaaacctact
     4621 tccctatatt cgttatggtc atcctggcca ccggcatcgg ggcgacgatg atcattctct
     4681 cggccatctt cagccgccgg actaagagtt tgaaaaagtt tacgccctac gagtgcgggg
     4741 tggacccggt cggctcggcc cgggagcgct ttccgatcaa gtactacatg gtggcgatgc
     4801 tttttatcat cttcgatatc gagacggtct tcctgtaccc ctgggcggtg gtgtacaagc
     4861 agttaggctg gttcggcttc atcgagatgg ggattttcat cctcctgctt ttcttgggat
     4921 acgtctacgt cctcaagaaa ggagcgttgc ggtgggatta gatgtcaaag agaaggcgat
     4981 agaggcgctc caggaagaac tgaaagacca ggtggcgctg accacagtgg agaaactcgt
     5041 caactgggcg cgaaaagggt cgctctggcc gctgggtttt gggctggcct gctgcgcctt
     5101 tgagatgatg agcgcccagg cctccaagtt cgacttatcc cgctacggga tggaactgat
     5161 gcgcccctcc ccccgccagg ccgacttgat gatcgtgacg ggacgcgttt ccaccaagat
     5221 ggcgccggtg gtgcggcagt tgtacgacca gatgcccaac cccaagtggg tgctctccat
     5281 gggcgcctgt gcctcaacgg gcgggatttt caacaactat gccatcgtcc agggcgtcga
     5341 taaaatcgtg ccggtggacg tctacgcccc ggggtgcccc ccgcggccgg agcagttgat
     5401 caatgccatt atggtgctgc gggagaagat cgcccgcgag aaaatttccg gccagaaaga
     5461 gaaaccgatt gtgatcggct ggcagcaggt gccggttaaa acataggtga taggtggcag
     5521 gtcaaaggtg gtagggaacg gattttgtag ccgtcccggc gtcaacttgc aacataggaa
     5581 gagatgactg aaccgaaata cgttacaaaa ttaaaagaaa agtttcccgg cgattttgtc
     5641 gaagtcaatg aattccgggg ggagtggacg gcggtcatcc gcaccgagag catcgtggat
     5701 ctctgtcgct tccttcgtga cgacccggac tgccagttca atttcctctc cgacctctgc
     5761 ggggtcgatc gactcaactt aaaccagacg ccgcgctttg aggtcgttta ccacctcttc
     5821 tcactgaagt tctaccaccg gctctgcttg aaagtgagca tcgaagaaac ccgggctgtg
     5881 ccttcggtca cgggggtctg gtcggcggcg aacaggcacg aacgggaagt cttcgat
//
LOCUS       DBNGZD010000415         5530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1939838, whole genome shotgun sequence.
ACCESSION   DBNGZD010000415 DBNGZD010000000
VERSION     DBNGZD010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1939838"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..641)
                     /gene="cobO"
                     /locus_tag="AC16GM_10795"
     CDS             complement(90..641)
                     /gene="cobO"
                     /locus_tag="AC16GM_10795"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR2924194.1"
                     /translation="MANEPKKERRGLVVIHTGDGKGKTTAALGMAVRAAGYKMKVFIL
                     QFVKGTWKYGELEGVKLLAPYVEMEQKGKGFVGIIDDKYDISEHQKAGREALVYARKI
                     VSEGEYDLVILDEINVAIGLSLIPVADVVELVKSKPARLHLVLTGRGAPQELIELADT
                     VTEMREIKHAFRRGILAQKGIDF"
     gene            complement(641..3319)
                     /locus_tag="AC16GM_10800"
     CDS             complement(641..3319)
                     /locus_tag="AC16GM_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR2924195.1"
                     /translation="MLPHLQKSFPREQLLSDALARSCYASDASIYAVTPQAVLLVKSE
                     ADVARAIDFANKHRISLTPRGAGSGLAGGALGEGLILDFSGYRGIVSLDSESKTVTVR
                     VGTIYEELNAFLKPHRLVFAPDPSSGDVCQIGGMLATNASGPRTVKYGTTKDYVLRMV
                     VFLHSGKRVELEKMEKGSERYRRFAAEHPEFATVENLVGKKSDLILIRWPKVKKNSSG
                     YNLYDFAAGLREGVVDYTKIFIGSEGTLGFFPEVTLRLVPLPDKRATVLAHFANLLPV
                     GDAVLELLPLGPSALEIIDGHALELVGREKYGVPRDSEMLLLVEFDGGDLKSLVQRAK
                     SILQKYPLSVPSVGEDDPVKQARLWNVRKAVLPTLYRYDPVKRPVGFIEDSCLPSEQV
                     PRFLRFLDGQFKRHNLQAGVYGHIGDGNVHIRPLLNLRDPQDFEKMRALYEDVYAEVL
                     RLGGSLAAEHGDGRLRAPRLKELYGKEIYDLFVKIKKTFDPQNIFNPGVKFAENEFTQ
                     NINLAKLDHHCAACGKCNTVCPSYDITRTEILGSRGWVRALAVDHLSNAEARLAIDAC
                     LNCKACMVVCPAGVDTSEIVRKRREKDRSPWKRLALGTLTHPSLLRTGATFVGRTQIF
                     WKFFLGSRNYPKFTGRAWSRRSNEGELDRAPVAYFPGCPDTFIANGVADAVLNVMDNL
                     GVRTIVPKTFCCGAPLETFGYPDLIKKNARKNLKLLEKFDAIVTSNGSCYWALKAFRD
                     CFAPGEPEHAMAQKFTERVFDFSEYIEKAAPQTFASGQRRMRVTYHDPCHLRAAGITL
                     EPRALLRRLPGVEFVEMPDAERCCGAAGAYRYLHYHEAMQIVGRKVRGIEAVKPDLVA
                     TGNSLCAFHIADGIHRKIPVLHVAQVVEKFLAEKGA"
     gene            complement(3392..4621)
                     /locus_tag="AC16GM_10805"
     CDS             complement(3392..4621)
                     /locus_tag="AC16GM_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2924196.1"
                     /translation="MSLLDLFKQVYVSLRANRMRSALTMFGIVWGIASVIILVGIGDG
                     FKAANQREFTQLGSNISILWGGRTSMQAGGRRGGKDIRFTEDDVKAVIAQASLVHQAS
                     PEIRRDLQIEYGPKSMATDVHGVYSAFGQMRSLVLESGRFLNQADFDVKRAFCVIGDE
                     VNRRLFSGENPVGKMIRIANRPFLVIGKLQKKDMGSRYGSPDDEQIFIPFSVAQQIFS
                     RKYINNIVISPRNPDEDEAALAEVKRILSRRLNFNPEDRDAVNIWNTARTARQVNTLF
                     FAIEILMTLIGGVTLGVGGVGVMNIMLVSVTERTREIGIRRALGARRRNVLFQFLSEA
                     LVLTFSSGLLGMAVGIGLTKLIASLPLPEGFDPPVVSPTVVVTSIILLTVVGVLSGTY
                     PALRASRLSPVEALRYE"
     gene            complement(4621..>5530)
                     /locus_tag="AC16GM_10810"
     CDS             complement(4621..>5530)
                     /locus_tag="AC16GM_10810"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2924197.1"
                     /translation="AGQPLVEIAPQATPVELVRAREAVNSAALDLELKKLDWQKADSL
                     YKRKLISQDESRRFRTQFDLAQSRHTAAAAELAILEQSEAGPNDTSHRMAQGADLQEE
                     DENAEVLRSTVVYAPFSGVVLSKNIDVGSSVSAVSAASGGSVVMTVADDSRMWFKGEI
                     DESDLGKIKLAMTAKIILDAYPDTFFTGILDWISPMGTEKDNLTTFQVRATLKNSTGR
                     LKAGMRGKGEIVLAEKKDVLWANEGAIIYNEGKTFVDLFDVKNPQKPKRQPIKTGISD
                     GLKTEILEGVKEKDTIIMPSGPSSEL"
BASE COUNT         1172 a         1678 c         1497 g         1183 t
ORIGIN      
        1 aggttggcag gctattgcac ccagaaaccg gccagcaaat cacgagcctg ctgcgcagga
       61 aatttcagca cctgccgacc cccggacgct tagaaatcga tccccttttg cgccaggatt
      121 ccccggcgga aggcgtgctt gatttcgcgc atttcggtca ccgtgtcagc cagttcaatt
      181 aactcctggg gcgcaccccg gcctgtgagc acgaggtgca gccgagccgg cttggatttc
      241 accagttcaa ccacgtcggc caccggaatc aaactgagcc cgatcgccac gttgatttcg
      301 tccaatatca ccagatcgta ttccccctcg gaaacgattt tccgcgcata caccagcgct
      361 tcccggcctg ctttttggtg ctcggaaatg tcgtatttgt cgtcgatgat cccgacgaac
      421 cctttcccct tctgctccat ttctacgtag ggtgcgagca gtttgacgcc ttccagttcg
      481 ccgtatttcc aggtgccttt gacgaactgc aggatgaaga ctttcatttt ataccctgcc
      541 gcgcgcaccg ccatccccaa ggccgcggta gttttcccct tcccgtcgcc ggtgtggatg
      601 acgactaaac cgcgccgctc ttttttcggt tcgttcgcca ttaagccccc ttctccgcca
      661 agaatttttc caccacctgc gccacgtgga gcaccggaat tttcctgtga atgccgtcgg
      721 cgatatggaa ggcgcagagc gaattgccgg tggccaccag atcgggtttg accgcctcaa
      781 tcccccgcac cttgcgtccg acgatctgca tcgcctcgtg gtagtgcagg tagcggtagg
      841 ccccggcggc gccgcagcag cgctccgcat ccggcatttc gacgaactca actcccggca
      901 accgccgcaa aagcgcccgc ggctcgagcg tgatccccgc cgcccgcagg tggcaggggt
      961 cgtggtaggt cacccgcatt cgtctttgtc cagacgcaaa tgtttgtggc gcagccttct
     1021 cgatatattc cgaaaaatca aaaactcgct ccgtgaactt ttgtgccatt gcgtgctccg
     1081 gctcgccggg agcgaagcaa tcccgaaagg ccttcagtgc ccagtagcag gagccgttgc
     1141 tggtgactat cgcatcgaat ttctccaaga gcttgagatt cttccgtgcg tttttcttta
     1201 tcaaatcggg gtagccaaac gtctccagcg gcgcaccgca gcagaaagtt ttcggtacga
     1261 tcgttcgcac acccaggttg tccatgacgt taagcacggc atcggccacc ccgttggcaa
     1321 tgaaagtgtc gggacagccg gggaagtacg ccacaggcgc gcggtcaagt tctccttcat
     1381 tagaacgccg tgaccaggcc cggcccgtga actttggata gttccgcgac cctaaaaaaa
     1441 acttccagaa gatttgcgtc cgcccgacaa aggttgcgcc ggtgcgcagg agcgagggat
     1501 gcgtcaaagt gcctaaggcc aggcgcttcc aaggcgaccg gtccttttcc cgccttttgc
     1561 gtacgatttc cgacgtatcc acgcccgccg ggcagaccac catgcaggct ttgcagttca
     1621 ggcaggcgtc aatagctaaa cgcgcttctg cattggagag atgatcaacg gccagcgccc
     1681 gcacccagcc gcgggaaccc aagatttcag tccgagtaat gtcgtaactg gggcagaccg
     1741 tgttgcactt cccgcaggcc gcgcagtggt ggtccagttt ggccaggttg atgttctgcg
     1801 taaactcatt ctctgcaaat ttgactccgg gattgaaaat gttctgcggg tcgaaagtct
     1861 ttttgatttt cacgaagaga tcataaatct ccttgccgta gagctctttt agccgcggcg
     1921 cgcggagacg tccatccccg tgctcagccg cgagcgaccc gcccaggcgc agcacttcgg
     1981 cataaacatc ttcatataac gcccgcattt tctcgaagtc ctgcgggtcg cggagattaa
     2041 gaaggggccg gatatggacg ttcccgtccc cgatatggcc gtacaccccc gcctggaggt
     2101 tatgccgctt gaactggccg tccaggaagc gcagaaaacg cggaacttgc tcagacggca
     2161 ggcaggaatc ttcgataaac cccaccggcc gcttgaccgg atcgtagcgg tacagggtcg
     2221 gaagcaccgc tttgcgtacg ttccaaaggc gcgcctgctt gaccggatcg tcttctccga
     2281 ctgatgggac agatagaggg tatttctgaa ggatgctttt tgcccgttga acgaggcttt
     2341 tgagatctcc gccatcgaac tcaaccaaaa ggagcatttc agaatcgcgc ggaacgccat
     2401 acttctcccg cccaactaac tcaagcgcgt ggccgtcgat gatttccaat gccgacggac
     2461 ccagcggcaa aagttccaaa accgcgtcgc cgacggggag caggttggcg aaatgagcca
     2521 gcacggtcgc ccgtttgtca ggaagaggca cgaggcgcag ggttacttcg gggaaaaaac
     2581 cgagtgtccc ttcgctcccg atgaaaatct tggtgtaatc caccactcct tcacgcaggc
     2641 cggccgcaaa gtcgtagagg ttgtacccgg aagaattttt cttcaccttc ggccagcgaa
     2701 taaggatcaa atcactcttt ttccctacca gattctccac cgtggcgaac tcgggatgct
     2761 ccgctgcaaa ccggcggtac cgctcactcc ccttttccat cttttctaat tcaacccgct
     2821 tccctgaatg gagaaaaacc accattcgga ggacgtaatc tttcgtcgtc ccgtacttga
     2881 ccgtcctcgg cccggaggcg ttcgtcgcca gcatccctcc aatctggcac acatcgccgg
     2941 atgagggatc gggtgcaaac accagccggt gcggcttcag gaacgcgttc agctcttcat
     3001 agattgtccc cacccgcacg gttacggttt tgctttcgct atcgagactg acaattcctc
     3061 gatagccaga aaaatcgagt attaagccct cccctaacgc gcccccggcc agacccgatc
     3121 ccgcaccgcg cggcgtgagt gaaattcggt gcttattggc aaaatcaatg gcgcgggcaa
     3181 catccgcctc ggatttaacg aggagcaccg cctgaggtgt taccgcataa atactggcat
     3241 ccgaggcgta gcaggagcgg gccagggcgt cggagagcaa ctgttctcga ggaaaacttt
     3301 tctggagatg gggcagcatt gcaccagaat atataggcag gtaaaacgaa aaatagcaaa
     3361 taatcggaga cgggaactac cggagccgga gctactcgta ccgtagcgct tcgaccggcg
     3421 aaagccggga agctcgcaaa gccgggtagg tcccggataa gacaccgaca accgtcaaga
     3481 ggataatgga agttaccacc accgtcgggg aaaccaccgg cgggtcgaac ccctccggca
     3541 gaggcagact cgcaatgagc ttggtcaggc cgatgccgac cgccatgccc aaaagcccgg
     3601 aagagaacgt caaaacgagc gcctccgaca aaaactgaaa gagcacgttc ctgcgcctgg
     3661 ctcccaaggc acggcggatg ccgatttccc gggtgcgctc ggtcacggat accagcatga
     3721 tgttcatcac ccccacacca ccgactccca aagtgacccc gccgattaaa gtcatcaaaa
     3781 tctcaatggc gaagaaaagt gtgttcacct gccgggcggt gcgggcggtg ttccagatgt
     3841 tcaccgcatc ccggtcttcc ggattgaaat tgagccgccg gctcaaaatc cgcttcactt
     3901 cggccaaggc ggcttcgtcc tcgtccgggt tccgcggcga gataacgatg ttgttgatgt
     3961 acttccgcga aaaaatctgc tgcgccaccg aaaagggaat gaaaatctgc tcgtcgtccg
     4021 gactgccgta acgggaaccc atgtctttct tctgcagttt gccgatcacc agaaacggtc
     4081 ggtttgcaat ccggatcatt ttccccactg gattttctcc ggagaagagc cggcggttga
     4141 cctcgtcgcc gatgacgcaa aaggcccgtt ttacgtcgaa gtcagcttgg ttcaaaaagc
     4201 ggcccgattc gagcaccaga ctgcgcatct ggccaaaggc ggaataaacc ccgtgcacgt
     4261 cggtggccat cgatttgggg ccgtattcaa tttgtaaatc gcgccggatt tccggggatg
     4321 cttggtggac taaactggcc tgggcaatga ccgctttgac gtcgtcctcg gtgaagcgga
     4381 tatccttccc gccacgccgg ccgcccgcct gcatagaggt tctcccgccc cagaggatgg
     4441 agatgttaga acctaactgg gtaaactccc gctggttggc ggctttgaag ccgtcgccga
     4501 tcccgaccaa gatgatgact gaggcgattc cccagacgat gccgaacatc gtcaaagccg
     4561 agcgcatccg gttggcgcgc aggctgacgt aaacctgttt gaataaatcc aacaacgaca
     4621 tcagagttca ctggacgggc cggagggcat gataatggtg tctttctctt tgaccccttc
     4681 taaaatttcg gtcttcagac cgtcggagat gccggttttg atcggctggc gttttggctt
     4741 ttgcggattt tttacgtcga agagatcaac gaacgtcttg ccctcgttgt agataatcgc
     4801 cccctcattg gcccagagga cgtctttctt ttccgccagg acgatttccc ccttgccccg
     4861 catccccgcc ttcagccgtc cggtcgagtt cttcaaggtc gcccgcacct ggaatgtggt
     4921 caggttgtcc ttttcggtcc ccataggaga aatccagtcc aaaatgccgg tgaaaaaggt
     4981 gtccgggtag gcatccagaa tgattttggc ggtcatcgcg agtttgattt ttcccaaatc
     5041 ggactcgtca atttccccct tgaaccacat ccgcgaatcg tcggcgaccg tcatcaccac
     5101 gctgccgccg gaagcggcgg agacggccga gaccgaggaa ccgacgtcaa tgtttttcga
     5161 cagcaccacg ccggaaaagg gggcgtagac caccgtgctt ctcagcactt cggcgttttc
     5221 atcttcctcc tggagatcgg caccctgggc catacggtgg ctcgtgtcgt tgggtccggc
     5281 ctccgactgc tccaaaatcg ccagttcggc ggctgcagcg gtatggcggg attgcgctaa
     5341 gtcaaactga gtcctaaacc ggcgggattc atcctgcgaa atcaatttcc ttttgtagag
     5401 ggaatcggct ttctgccagt ccaatttctt gagttccagg tcgagcgctg cggagttcac
     5461 cgcctcgcgc gccctcacta attccaccgg cgtggcctgc ggcgcgattt ccacgagcgg
     5521 ctggcccgcg
//
LOCUS       DBNGZD010000416         3512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1038688, whole genome shotgun sequence.
ACCESSION   DBNGZD010000416 DBNGZD010000000
VERSION     DBNGZD010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1038688"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="AC16GM_10815"
     CDS             <1..941
                     /locus_tag="AC16GM_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR2924198.1"
                     /translation="AFRGPALFSVFSGATPNAADLPVYLVAAAAMESRAFPAFVYDPA
                     LGPDWASRFHLEANPQADQDWPVQKFAYEDEGHQRISQDLAFTVVDFVACDRRYARHF
                     ARIPRAKWNANLVPVGDCLGRDTTGLPGKVPYLLLVDRNNVLQKVQVDDQLMREARRC
                     RELWHSLQELGGIHNSHAERLLARERKAQSERERGAAEARDHGPKPVATPSATTSLAP
                     PAAAAEAQPEKSSDEPYIETPRCTSCNECTNINDKMFAYNENKQAFIADLDAGTYAQL
                     VEAAESCQVAIIHPGKPRNPNEPGLEELVRRADAFR"
     gene            1879..>3512
                     /locus_tag="AC16GM_10820"
     CDS             1879..>3512
                     /locus_tag="AC16GM_10820"
                     /inference="COORDINATES: protein motif:HMM:NF040218.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HIR2924199.1"
                     /translation="MFSALRSLVRRCPGWAAGAIGIAFLVLVSPFSSAFSQVNLLAQK
                     DGAVLDDRLGWSVAGAGDVNGDGVADYIVGAPYTDPGGVATAGSAFVYSGTSNALLYQ
                     KDGVTAGDRFGTAVAGAGDLNGDGRADFIVGSLSTAFVYSGATGTLLYQKTGGSAVAG
                     AGDLDGDGRADFMVGVAGADPSGVTDAGSVFVYSGATGNLLYQKNGVASGDNFGAAVA
                     GVGDLNGDGRADFIVGAPGFDPGGLFNAGSAFVYSGATGALLYQKNGAASVDLFGNGV
                     AGAGDVSGDGRADFIIGARLADVGGLTDAGSAFVYSGATGALLYQKNGSAVGQGLGRS
                     VAGAGDVNADARADFIIGASDASPGGLTNAGSAWVFSGITGALLYQKDGAAAYDNLGF
                     SVAGAGDVSGDGVADFLIGVPFADPGGRFDAGSALVYGFTTTPPPSAVISLSPTSFSF
                     SAVQSGSLPPSQSLQITNSGGGSLNWSVVKAGNSSWLSATPASGTAPSTVVVSITTTG
                     LAPSTYYDTLVISGNASNSPQRVPVTYTVSAPPSPAV"
BASE COUNT          667 a         1011 c         1088 g          746 t
ORIGIN      
        1 tggcctttcg cgggccggcg ctgttcagcg tattttccgg cgcgacccct aacgccgcag
       61 accttcccgt ttatctggtt gcggcggcgg cgatggagtc gcgcgccttc ccggcgttcg
      121 tttatgaccc cgccttgggc cccgactggg cgtcgcgctt tcatctcgaa gccaatcccc
      181 aggcggacca ggactggccg gtccaaaagt tcgcctacga ggacgaaggg caccagagga
      241 tctcccagga tctggccttc acggtggtcg atttcgtggc ctgcgaccgc cgttatgcaa
      301 gacattttgc gagaatcccg cgggcgaagt ggaatgcgaa cctcgtcccc gtcggcgact
      361 gtttaggccg ggacacgacg ggcctgccgg gaaaggtccc ctatctcctt ttggtcgatc
      421 gcaataacgt gctgcagaag gttcaggtcg acgaccagct gatgcgcgag gctcgccgct
      481 gccgcgaact gtggcacagc ctgcaggaac tgggcggcat tcacaactca cacgccgaac
      541 ggctgctcgc gcgcgagcgg aaggcccaaa gcgagcgtga acgcggcgcg gccgaggctc
      601 gggatcacgg accgaagccg gtggccacgc cgtcagcaac aacctcgctg gccccgccgg
      661 ccgcggcggc ggaagcgcag ccggagaagt cgtccgatga gccctatatc gaaacacccc
      721 gctgcacgag ctgcaacgag tgcaccaaca ttaatgacaa gatgttcgcc tacaacgaaa
      781 acaaacaggc gttcatcgct gacctggacg ctggcaccta tgcgcagctc gtggaggcgg
      841 cggagagttg ccaggtagcg atcattcatc cgggtaagcc gcgcaacccg aacgagccgg
      901 ggttggaaga gctggtgcgg cgggctgacg cctttcggta gccgcacgca acacttctgc
      961 cagttccggt cgccgactgg cgggggggcg atgagcaagt tttccggact tgccgccaaa
     1021 gggcgggctc cggtatgagg ccgtgcccgg ttattcgcca agcaccaaca agacaaccgc
     1081 ccgagcccgg cgcggaaccc cgtgaccgcc gtcattaatt cttggcgatt ccgacttttt
     1141 gtcccaggag aaattataat gaagccggtc gtctcggaag gccggattta ctacgtaacc
     1201 gaaggcaaaa ccatccctct aacttactgc aattcgacga ccgagttcca ccagtcaaaa
     1261 atagcccctc agggctacga ccaactcttg gaattcacta taccattttc gatcaagaca
     1321 gccagattgt gtcggggagg aataaaacag tttttgaagt cccaaaaaag gcaagagata
     1381 tagcttctct ggaatggacc gaattggttt aactcgatgg gcgctttttc cgacaaggga
     1441 agtcgatatt gaactcccag caatacaaag taaaactttc agtgattgag ccaaattttc
     1501 tcgtgtgaaa ctgtttacga atcatcaagg ttcagggtaa atgattacca ctttaagtta
     1561 aagaaaccgt aggtgacgtc agattgaaat tgactcattg ggctatcctg ctccaggact
     1621 ctgctgtaaa ggcaggtgta gatttagtta aagtttgagt tatcacctgg cgatactgaa
     1681 gtcgtaggat taagatagcc gcattcttca cgtactggca gtttcccctc catcggtcga
     1741 catcgttaag cggcggaagc ctggatccgg ttcttactga gacctgaaaa actcccggat
     1801 ggacttacca acgcggtaag acatggcagt gcagaaatgc atagctgggg tgaagaagaa
     1861 ctcttgaaag gaggccgtat gttcagtgct cttcgttctc tcgtgcggcg ctgtccggga
     1921 tgggcggcag gagccattgg catagccttc ctcgttctgg tttctccttt ttcctccgct
     1981 ttcagccagg tgaatctgct tgcgcagaaa gacggggcgg tactggatga tcggctgggg
     2041 tggtcggtgg ccggcgccgg ggacgtgaat ggggacggtg tagctgatta catcgttgga
     2101 gcgccctata ccgatccggg aggagtcgcc actgccggct ccgccttcgt ctactccggc
     2161 acttccaacg ccctcctcta tcagaaagac ggcgtcaccg ccggggaccg cttcgggact
     2221 gcagtagccg gagccggaga cctgaacggt gacggccgcg ccgatttcat cgtcggttcg
     2281 ctctccaccg ccttcgtcta ctccggtgcc accggaactt tgctctacca gaagaccggc
     2341 gggtcggcgg tggccggggc cggggatctg gatggtgacg gccgcgctga tttcatggtc
     2401 ggtgtggcgg gtgctgaccc gagcggagta accgatgccg gttcggtttt cgtctactcc
     2461 ggggctacgg gtaatttact ctaccagaaa aacggtgtcg cctccgggga caacttcggc
     2521 gctgcggtgg ccggggtggg tgatctgaac ggggatggcc gggccgattt catcgtcggt
     2581 gctcccggtt ttgatccggg ggggctcttt aatgccggtt cggccttcgt ctactcgggt
     2641 gccactgggg cgcttttgta tcagaagaac ggcgccgcca gcgtggatct cttcgggaac
     2701 ggggtggccg gggcgggaga tgtcagtggt gatggccggg ccgatttcat catcggcgcg
     2761 cgtctggcgg acgtgggggg gctgaccgat gctggctccg cctttgtcta ctccggggcc
     2821 actggtgcgc tcctgtacca gaaaaatggc agcgctgtcg gtcagggcct gggccgttcg
     2881 gtggccgggg ctggtgatgt gaatgctgac gcgcgagccg atttcatcat cggcgcttcg
     2941 gatgccagcc cgggcggcct gactaatgcc ggctcggctt gggttttctc cggaatcact
     3001 ggggcactgc tgtaccagaa agatggcgcc gcggcctatg acaatctggg cttttcggtc
     3061 gccggagctg gcgacgtgag cggcgatggg gtagccgact tcctcatcgg cgtgcctttt
     3121 gccgatccgg gtggacgttt cgatgccggt tctgctttgg tctatggttt tacgacaacc
     3181 cctccaccca gcgcagtcat atcgctttcg ccgacttcct ttagttttag tgccgtgcag
     3241 tcggggtctt tacccccttc gcagagcttg cagattacca actccggggg cgggagtttg
     3301 aactggtctg tagtcaaagc cggcaattcc agctggcttt cggccactcc ggcgagcggg
     3361 acggctccga gcacggtggt ggtttccatc accaccaccg gcctggctcc cagtacctac
     3421 tacgacacgc tggtgatttc cggcaacgcc agcaattcgc cccagcgggt gccggtaacc
     3481 tacacggttt cggctcctcc aagccccgcc gt
//
LOCUS       DBNGZD010000417         9838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1246097, whole genome shotgun sequence.
ACCESSION   DBNGZD010000417 DBNGZD010000000
VERSION     DBNGZD010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1246097"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /locus_tag="AC16GM_10825"
     CDS             complement(<1..2087)
                     /locus_tag="AC16GM_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2924200.1"
                     /translation="MSLVIILFGVISFTRLPVREYPDIDPPVVSISTFYRGASPNVVE
                     TEITDVLEEQLSTLEGVKTITSSSREQGSGITIEFELGRDVDQAANDVRDRVARIRGR
                     LPREADDPIIQKVDVNAQPIVWLALSSTRHSNLEVSDVADRVLKERLQRLPGVGAVFI
                     GGERRYAMRVWLDPQRMAAHGLTTQDVENAIRRENTEIPGGRVEGRGREFSVRTLGDL
                     STPEEFAAIIVGQAGDDLVRLGDVAEVSLGAADERTAVRYNGQPAVGLGISKQTKAST
                     IDVAEQIVGAAPALAKLLPAGMKLEVAYNSATFIQEAIDDVTRTLMVAMLLVVLVILV
                     FLKSLRATIIPALAIPTSIVGSFAVAYFLGFTLNILTLLALVLAIGLVVDDAIVVLEN
                     VYRHMQLGKDRRKAAFDGSKEIGFAVLSITIALVAVFIPLSFLTGNVGRLLSEFGISV
                     AAAVLISGFTALTLTPMLASRMLKPLFGAGQSWASRSFDAFFARLDQTYGRILHRALE
                     NRWKVVAGAAVLVLLSLTLFKILPSELVPTEDRGIGFGVVIAPEGATLDYTDRYMHQV
                     EDILMKLPERQGLFTATGLGFGGPGRVTNGFVFLRLKPRAERDKSQQQIVQELFPRLI
                     SIPGVLAFVMNPPSLGASFASKPVEYVLQGDTYEELQRAVGVLMAKAAQLGYLINLDT
                     DLRLNKPQLDIKI"
     gene            complement(2132..3103)
                     /locus_tag="AC16GM_10830"
     CDS             complement(2132..3103)
                     /locus_tag="AC16GM_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2924201.1"
                     /translation="MPVEAVTVTSGTVTDRFEAVGTVEALEAVTVVSQIDALVMEIPF
                     REGSLVEKGILIAQLDDAQLRAEEARTEALRDQRKISYERIKSLVGQKLGAPQDLDDA
                     AAGLKVAEADLDLIRARLAKTRIVAPFSGLVGARRVSPGAFVRTGEPIADLAQIDELR
                     VTFYAPERYVADLRPGASVTVSTTAYPGYERKGKISVVEPVVNPQTRSVKILARVANP
                     QRRFRPGMSANVSAVLSERKNALLVPDEAIFAEGDQNLVYVIKSDSTVSRVAVTLGTR
                     LKNAVEVTEGLSPGTTIVRAGHQKLFEGARVFPVQPEQAPSPAGGKS"
     gene            complement(3404..4120)
                     /locus_tag="AC16GM_10835"
     CDS             complement(3404..4120)
                     /locus_tag="AC16GM_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR2924202.1"
                     /translation="MKLQTANGAELGYGAFELKRSYQKNLGRAFAFAAALHLVLIGGV
                     VFYQYLKARGAPTRIIEIKSLADLGPPPSLTNAPPPVPITQPKVTPPAIGIPKPVPDE
                     EAQPEVQIASQEELAKIESPVMSSGGDSLAIHVDPSELAPADFVPHEVEPEFIQRVEP
                     KYPELAQRSGVTGKVWVKVWVDKAGKVRDVKVYKSSGNALLDESAVEAARQCLYKPAI
                     QNGQPVAVWVTYPYEFTLHR"
     gene            complement(4124..4840)
                     /locus_tag="AC16GM_10840"
     CDS             complement(4124..4840)
                     /locus_tag="AC16GM_10840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR2924203.1"
                     /translation="MKLATVNGAPLGYGAFELKRFYQKNLGTGFALAAALHLVLIGGV
                     VFYQYLMSRGELARIIEIKSLADLGPPPSLTNVPPPVPITQPKVTPPAIGIPKPVPDE
                     EAQPEIQIASQDELAIIESPVMSSGGDSLAINIDPSELAPGSFVPHQVEPVMIYKVEA
                     KYPDLAIRSRVEGKVVVWVVVDKKGKVAKAQVWSSSGNALLDEAAVDAARQCLYKPAI
                     QNGQPVPCPVSYRIEFTLPR"
     gene            complement(4837..5373)
                     /locus_tag="AC16GM_10845"
     CDS             complement(4837..5373)
                     /locus_tag="AC16GM_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR2924204.1"
                     /translation="MAGVDIGGGRPQRKQRLRRPKRRLGIRIDMTPMVDIAFLLLIFY
                     MVTTIFAQPQAMEINLPKDEKKDIEIAESNLLILRVAEDGKLYWNFGTKTPEELPFTE
                     LRKWLLDWNRAKPKLNTLIKIHKKAKYHNLVDIIDEIQVSERLLKEANPAFSYRFAIA
                     PWDDNDDKQIAKATGGGG"
     gene            complement(5450..5932)
                     /locus_tag="AC16GM_10850"
     CDS             complement(5450..5932)
                     /locus_tag="AC16GM_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR2924205.1"
                     /translation="MLKRPKRRVAIRIDMTPMVDVAFLLLIFYMSTTQFKPPETKNVA
                     LPSSHQKAKLPDKDLVKVTVTKDDSIFVDYIEVREEKSADGSTFEVPTRVYEEATPAT
                     IASVINTIRLRNPRVFVVIRADKDTDYGTMEAMMKTMKENQLNRFQLLTEFEEEAEAS
                     "
     gene            complement(5932..6660)
                     /locus_tag="AC16GM_10855"
     CDS             complement(5932..6660)
                     /locus_tag="AC16GM_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR2924206.1"
                     /translation="MKQSIFITVLLIVALAVGYAVWEYLLPDYLKLGGPLVGLLIALS
                     IMLVAFIVERMLTLRKAKGHSAIPAYFKSVVEFLRKGDFEHALAACDKQRGSCANVLR
                     AGIDRYRQMAGNEPSAEKRLAETQRAIEEANALEVPLLERNLIAMSTIASIATMVGLL
                     GTTIGMILAFRATGGHGGVIDAPQLAVGISEALGTTAGGLFNAIVGIVAYNFFVNKVD
                     SFNYTIDEASYEVLQLLKERETSR"
     gene            complement(6725..7441)
                     /locus_tag="AC16GM_10860"
     CDS             complement(6725..7441)
                     /locus_tag="AC16GM_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924207.1"
                     /translation="MSDPQKAVKEKRDTSSAPEAAKVLDPDLRFRYIGFEVHSPKIKN
                     FWKSAEEEKQYLEKIRREGSVWLKARSLVNVAIFSAADRALVTAISLVVLGSLFLPWA
                     SGAQRLWALPLFGELATHLSQAAGPVLLAAAVLGLALVATVPVVALANLVSVWRRGPT
                     EEKYAFRLRRVLLGNWLPIILWLLLLVGALVGAPLSAGAGAWVQAARFDFMAVMRTIG
                     LGLWVALGGHLVLAMKANEL"
     gene            complement(7438..9252)
                     /locus_tag="AC16GM_10865"
     CDS             complement(7438..9252)
                     /locus_tag="AC16GM_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2924208.1"
                     /translation="MRRFSFDKNPGGQYIRPFMFVLRDPGKSLWSKRAIRSLLFGFFW
                     LGISVGGAVQAFGQNGSAAALAAYERKDYAAAVELFKQLAAKNPADPENYSYLGNCYY
                     HLGKFSDALAQYQAALSRKAKHWDALYGSGLTYLKLGQPGEAEQTFKKGTGDRAQKAR
                     FFNGLGFALQAQRKLDEADKAFRQAILANPKVAEYHLNLAEVNFDNGIYPIAVDEYRQ
                     ALVLDATLSEARYRLAQALLKGRDFNGALEQLKEVLRADSTFAPAYFEMGDLYLKNTP
                     PSFKEAIWSLEHYIKLDTTNLSKAYLALAKAYYALRFYDSTMVKAKSVIARDPKNLEA
                     YFTLGQAASEGQKWSEAVEAYKRYQELLTEGKPDYPYSAKDAEFFQKRGEAYVGLKDS
                     TARPLAIADFEKALLLDSSRTQVFAPLGSAYIQSGDYERAIAWYQRALAADSTKFSTW
                     YNIGLAYQQRLRFAEAAEAFRHAVALKPDNVPARSRLAYILTVNLQNYGEGRQAFLEV
                     LKVDSLSADAHLYIGFAYLVEKKAADALPYLERSYHLRVNHQAKSPCDNTDNLLWLAQ
                     ASALSNRIDEAKKWAKKALECDSKNQPARELLKSLEPR"
     gene            complement(9245..9466)
                     /locus_tag="AC16GM_10870"
     CDS             complement(9245..9466)
                     /locus_tag="AC16GM_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924209.1"
                     /translation="MGQVWVWTSYLILALIFGVGLFVLFSPGFARSFPYQSGVRILVG
                     LLLISYAGVRVWLTARRAAQSRAEGKWHA"
     gene            complement(9639..>9838)
                     /locus_tag="AC16GM_10875"
     CDS             complement(9639..>9838)
                     /locus_tag="AC16GM_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2924210.1"
                     /translation="PIANMPSAVQYITYLIPLRYFVEIVRRIFLKGTGIGQLWDQVLP
                     LFIFGLILLGASVWRLQRREL"
BASE COUNT         2015 a         2964 c         2816 g         2043 t
ORIGIN      
        1 tcgattttga tatcgagctg gggtttgttc aggcgcaggt cggtgtcgag gtttatcaga
       61 tagccaagtt gcgcagcttt ggccatcagc acaccgaccg cccgctgcaa ttcctcatag
      121 gtgtcccctt gcagcacata ctccaccggc ttggaggcaa aggaagcgcc cagactgggg
      181 ggattcatca caaaagccag aacgccggga atggagatga gccgggggaa aagctcctgc
      241 acgatttgct gctgcgattt gtcccgttcg gcgcgcggct ttaagcgcaa aaagacaaac
      301 ccgttggtaa cgcgaccggg gccgccaaac cccaagccgg tggcagtgaa aagtccctgg
      361 cgctcgggga gcttcatcag aatatcctct acctggtgca tgtagcggtc ggtgtagtcc
      421 aaagtcgctc cctccggagc gatgacgacg ccgaaaccga tgccgcggtc ctcggtcggc
      481 accaattcac tgggaaggat tttgaacaag gtcagcgaga gcaggaccaa aacagcggca
      541 ccggcaacca ccttccagcg gttctccagg gcccggtgca aaatccggcc gtaggtttgg
      601 tctaacctgg cgaagaaagc gtcgaatgag cgggaggccc agctttgacc cgcaccgaaa
      661 agcggtttga gcatccggga agccagcatc ggggtcaaag tcagagcggt gaaaccggaa
      721 atcaaaacgg cggccgcgac agagatgccg aactcgctta aaagccgccc cacgttgccg
      781 gtcaaaaacg aaagcgggat gaagaccgcc accagcgcaa tggtgatgga taaaacggca
      841 aagccgattt ccttggaacc gtcgaaggcg gcctttctgc ggtcttttcc cagctgcata
      901 tggcgataga cgttttccaa aaccacgatg gcgtcatcaa ccaccaagcc gatggctaaa
      961 accagagcta aaagcgtgag aatgtttaag gtgaaaccca agaaataagc gacggcaaaa
     1021 cttccgacga tggaagtcgg gatggctaaa gccggaatga tggtggcgcg caggcttttt
     1081 aagaagacca aaataaccaa aaccaccaaa agcatcgcta ccatcagcgt tcgggtgaca
     1141 tcgtcaatcg cctcctgtat aaaggtggcg gagttgtagg ccacttccag cttcatcccc
     1201 gcgggcagaa gtttggccag ggccggggcg gccccgacga tctgctccgc cacgtcgatg
     1261 gtgctggctt tggtttgctt actgatgccc aaccccacgg ccggctggcc gttgtaccgc
     1321 accgcggtcc gttcgtcggc ggctccgagg ctcacttcgg ccacatcccc caaccgcacc
     1381 agatcgtcgc ctgcctgccc gacgattatg gccgcaaatt cttccggggt ggacaaatcg
     1441 cccagcgtgc ggaccgaaaa ttcccgcccc cgcccttcca cccgtccgcc gggaatttcg
     1501 gtattctcgc ggcggatggc gttttccacg tcctgggtcg tcaggccgtg cgccgccatc
     1561 cgctgcgggt caagccaaac ccgcatcgcg tagcgacgct cgcccccgat gaagacagcg
     1621 ccgacaccgg ggagacgctg cagccgctcc ttcaaaaccc ggtcggccac gtcgctcact
     1681 tctaagttcg aatgccgcgt gctggagagc gccagccaga cgatcggctg ggcattgaca
     1741 tccacttttt gaatgatggg atcgtccgct tcgcgcggga ggcggccccg gatgcgggca
     1801 acccggtcgc gcacgtcgtt ggcggcctga tcgacatcac gccccaattc gaactcgatg
     1861 gtgatgcccg agccctgttc ccggctggag gaggtgatgg ttttcactcc ctccaaagtg
     1921 gaaagctgtt cttccagaac gtcggtgatt tcggtttcca ccacgttggg gctggcgccg
     1981 cggtagaagg tcgaaatgga caccaccggc gggtcgatgt ccggatattc acgtaccggc
     2041 agtcgggtga aggaaatcac cccgaaaagg atgatgacca gactcatcac cgaggcgaaa
     2101 accggacgtt tgatggatat ttcgcttatt ttcaggactt ccccccggcg ggcgagggag
     2161 cttgctctgg ttgcaccgga aaaactctgg ctccttcaaa cagtttctga tggccggcgc
     2221 ggacgatggt cgttcccggc gaaagccctt cagtcacttc gaccgcattt ttcaggcgcg
     2281 tgcccaacgt cacagccacg cgggagacgg tgctgtccga cttgatgacg taaaccaaat
     2341 tctggtctcc ttcggcaaat atcgcttcat caggaaccag aagggcattt ttgcgctcgc
     2401 tcaaaacggc ggagacgttg gccgacattc cggggcggaa gcggcgctgg gggttggcca
     2461 cccgcgccag gattttgacc gagcgggttt gcgggttcac caccggctcg accacgctga
     2521 tttttccttt ccgttcgtaa ccggggtagg cggtagtcga aacggtgacc gaggcaccgg
     2581 ggcgcaagtc ggcgacgtag cgctccgggg cgtagaaagt cacgcgcaac tcgtcaattt
     2641 gggctaagtc ggcaatcggt tcaccggtac gcacgaaggc tccggggctt acccggcgtg
     2701 cccccaccaa accgctgaaa ggagcgacaa ttctggtttt ggccaagcgg gcgcggatca
     2761 ggtccaggtc tgcttcggct accttcaacc cagcagcggc gtcgtccaaa tcctgcgggg
     2821 cgccgagttt ttgccccacc agcgatttga ttctctcgta gctgatttta cgctggtcgc
     2881 gcagcgcttc ggtgcgcgct tcttcggcgc gcaactgggc gtcgtccaac tgggcaatga
     2941 ggattccctt ttccaccagc gagccctcgc ggaacgggat ttccatcacc agggcatcga
     3001 tttgggaaac cacggttacg gcttccagcg cctccaccgt gccgactgct tcgaaccggt
     3061 cggtcaccgt tccggaagtt acggtgacgg cctccaccgg catcggcggc ggggcgaatc
     3121 cgcctccctg gtttttagag cagcccgcaa taagtaccag tgcgaaaaga aacgtcggag
     3181 tccaataggg agttttcaac tttctcgtct cgatgagcct actttttccc gttaattggt
     3241 cttagcccgt ctatacgatc tgacggaagc ttcgttggtc cagtagcaac gtgttggttt
     3301 taatcaacgg gagttgagaa ttgaatccgc ctgaattcca aataggaaga cctggcctga
     3361 cttccctgaa taatcaagtt cgtagctgga atgtttatac ctactaccgg tgcagcgtga
     3421 attcataggg ataagtcacc cagaccgcca ccggctggcc gttttgaatg gccggcttgt
     3481 acaggcactg gcgcgcggct tccacggcgg attcgtccaa gagcgcgttc ccggaagact
     3541 tataaacttt tacgtcccgg actttccccg ccttgtcgac ccacaccttc acccacacct
     3601 taccggtcac gcctgaccgc tgggccagtt ccggatattt cggctcgacc ctctggatga
     3661 attccggctc gacttcgtgc gggacgaaat cggccggcgc cagttcggac ggatcgacgt
     3721 ggatcgccag cgaatcgccg ccggagctca tgaccgggct ttcgattttc gccagttcct
     3781 cctgcgaagc gatttgaact tccggctggg cttcctcatc cggcaccggc ttgggaatcc
     3841 cgatggccgg cggcgtcacc ttgggctggg tgatcggcac cggcgggggc gcgttggtta
     3901 atgacggggg cggacccagg tccgctaagc ttttaatctc gatgatgcga gtcggcgcgc
     3961 cccgcgcctt gaggtactgg tagaaaacga ctccgccgat gagcacgagg tgcagggctg
     4021 cggcgaaggc gaacgctctg cccagattct tctggtagct gcgcttcagt tcaaacgcgc
     4081 cgtaaccgag ctctgcaccg ttcgcggttt ggagtttcat aggttacctg gggagagtga
     4141 attcaattcg gtaagacacc gggcacggca ccggctgacc gttctgaatg gccggtttgt
     4201 atagacactg gcgcgccgca tcgacggcgg cttcgtccaa cagggcattt ccggaagaac
     4261 tccaaacttg agcttttgct actttaccct ttttatcaac cactacccaa acaacgactt
     4321 ttccttcaac cctcgaacgg atggctaggt cgggatactt tgcttctacc ttatagatca
     4381 ttaccggctc gacttggtgg ggcacaaaac tacccggcgc cagttcggac ggatcgatgt
     4441 tgattgccag tgaatccccg ccggaactca tgaccgggct ttcgattatc gccagttcgt
     4501 cctgcgaggc gatttggatt tccggctggg cctcctcatc tggcaccggc ttagggatcc
     4561 cgatggctgg cggtgtcacc ttgggctggg taatcggcac cggcggtggc acattggtca
     4621 aggatggcgg cggacccagg tccgccagac tcttgatctc gatgatgcgc gccaactccc
     4681 ctcgtgacat cagatactgg tagaacacga cccccccgat gagtacgagg tgcagggccg
     4741 cggccaatgc gaaaccagtc cccagattct tctggtagaa gcgcttgagt tcaaaagcac
     4801 cgtagccaag cggcgcaccg ttgacggttg caagtttcat ccgccacctc ccgtcgcctt
     4861 ggcaatttgc ttgtcgtcgt tgtcatccca gggcgctatc gcgaaccgat acgaaaacgc
     4921 ggggttggcc tctttcaaaa gccgttccga cacctgaatt tcgtcgatga tgtccaccag
     4981 gttatggtac ttggccttct tgtggatttt gatcaacgtg tttagcttcg gcttggcccg
     5041 gttccagtcc agaagccact tgcgcagttc ggtgaacggc agttcctccg gcgttttcgt
     5101 gccgaagttc cagtagagtt tcccgtcctc ggccactcgc aggatcagga ggttcgattc
     5161 ggcgatttca atgtcttttt tctcatcctt gggcaggttg atttccatcg cctgcggctg
     5221 ggcgaaaatg gtggtcacca tgtagaagat caaaagcaga aaggcaatat cgaccatcgg
     5281 ggtcatgtcg atcctgatcc cgaggcgccg tttgggacgc cggagcctct gcttgcgctg
     5341 gggtctcccg ccgcctatgt ctacaccggc catctgctac tcctcactgg taatacgcac
     5401 caagtccacc tttattcccg gcccggctga ccagggtgcc cccacgccgc tacgaggcct
     5461 cggcctcctc ctcaaattcg gtcaaaagct ggaagcggtt gagctgattc tccttcatcg
     5521 ttttcatcat cgcctccatg gtgccgtagt ctgtgtcctt gtcggcccgg atcaccacga
     5581 aaacccgggg gttgcgcagc cggatggtgt tgatgaccga ggcaatggtc gcgggcgtcg
     5641 cctcttcgta gacccgcgtg ggcacttcaa aggtggatcc gtcggccgac ttctcttcgc
     5701 gcacttcgat gtaatcgacg aaaatcgagt cgtccttggt cacggtcacc ttcaccagat
     5761 ccttatccgg cagtttcgct ttctggtggg aggagggcag ggccacgttc ttggtctccg
     5821 gcggcttgaa ctgggtggtg ctcatgtaga aaatgagcag aagaaacgcc acgtccacca
     5881 tgggggtcat gtcgatccgg atcgccaccc ggcgtttggg ccgcttgagc atcagcggga
     5941 ggtctcccgt tccttgagca gctgcagcac ttcgtaggag gcctcgtcaa tggtgtagtt
     6001 gaaactgtcg actttgttca cgaagaagtt gtaggccacg atgcccacga tggcgttgaa
     6061 caacccgccg gcggtggtcc ccagggcctc ggaaattccc acggccagct gcggcgcgtc
     6121 gatcacgccg ccgtgcccgc cggtggctcg gaaggcgagg atcatcccga tggtggtacc
     6181 cagaagcccc accatggtgg caatcgaggc gatggtggac atggcgatca ggttccgctc
     6241 caaaagcggc acttccagcg cattggcctc ttcgatggcc cgctgcgttt cggctaaccg
     6301 tttttcagcc gaaggttcgt tgccggccat ctgccggtag cggtcgatgc cggcgcgcag
     6361 gacgttggcg caggagccgc gctgcttgtc gcaggccgcc agcgcgtgct caaagtcgcc
     6421 tttccgcagg aattcaacga cgcttttgaa atacgcggga atggcggagt ggcctttggc
     6481 tttccgcagg gtgagcatcc gctccacgat gaacgccacc agcatgatcg aaagcgcgat
     6541 taaaagcccc accagcgggc cgcccagctt gaggtagtcc gggagcaggt actcccacac
     6601 ggcatacccc acggccagcg ccacgatcaa caggaccgtg atgaagatcg attgtttcac
     6661 tccttaccct cctactgtgg tttttctgct ctagttcatt acgccagacg tttgttcgct
     6721 cagatcacaa ctcattggcc ttcatggcca gaaccaggtg cccgcccagg gccacccaga
     6781 gcccgaggcc gatcgtccgc atcacggcca taaaatcaaa acgcgccgcc tgcacccacg
     6841 caccggcgcc ggcggacaag ggagcaccga ccaaggcacc gaccaaaagc aacaaccata
     6901 aaataatcgg cagccagttc ccgagcaaaa ctctccgcaa ccggaacgcg tatttttctt
     6961 cggtggggcc ccgccgccag acggagacca gattcgccag ggccacaaca ggaactgttg
     7021 caacgagcgc cagaccgaga accgcagccg ccagaagcac cggtccggcc gcctgactca
     7081 gatgggtagc cagttccccg aaaaggggca gtgcccagag acgctgggca cccgaggccc
     7141 acggcaggaa aagacttccc aaaaccacaa gcgagatggc agtaaccagc gcccggtccg
     7201 ccgcggaaaa tatggcgaca ttcaccaggc tgcgcgcttt gagccagacg ctcccctcgc
     7261 gccttatttt ttcgaggtac tgcttttcct cttccgcgga cttccagaaa tttttgattt
     7321 tcggcgaatg aacttcaaag ccgatgtacc tgaaccggag gtccggatcg aggaccttgg
     7381 ccgcctcagg cgcggaggac gtatcccttt tttctttgac cgctttttgc ggatcgctca
     7441 tcgcggttct aatgatttta aaagttcccg cgccggctgg ttcttcgaat cacattccaa
     7501 ggccttcttg gcccactttt tggcctcgtc aatccgattt gacagggcgc tggcctgggc
     7561 caaccagagg aggttgtcgg tgttgtcgca ggggcttttg gcctggtggt tgacccgcag
     7621 gtggtaggac ctttccaggt agggcaaggc gtcagccgcc tttttctcaa ccagataggc
     7681 aaacccgatg tagagatgcg catccgccga aagcgaatca actttcagca cttccaaaaa
     7741 cgcctgccga ccttcgccgt agttctggag gttcacggta aggatgtagg ccagacggga
     7801 gcgcgccggc acgttgtcgg gcttcagcgc cacagcgtgg cgaaatgcct cggccgcttc
     7861 ggcaaagcgc aaccgttgct ggtaggccaa accaatgtta taccaggtgg agaatttggt
     7921 tgaatccgcg gccaaggccc gctggtacca ggcaatcgct ctctcataat ctccactctg
     7981 gatgtatgcc gaccccagtg gagcaaagac ctgtgtacgg gaggagtcga gcaaaagcgc
     8041 cttctcaaaa tcggcgatgg ccaggggccg ggcggtggaa tctttcaggc cgacgtaggc
     8101 ctcgccgcgc ttctggaaaa actcggcatc cttggcgctg tacgggtagt cgggctttcc
     8161 ctcagtgagc agctcttgat accgcttgta ggcctccacc gcctcgctcc atttttggcc
     8221 ttccgaggcg gcctgcccca aggtgaagta cgcctcgagg ttcttcgggt cgcgggcgat
     8281 caccgactta gctttcacca tcgtggagtc gtagaagcgc aaggcgtagt aagctttggc
     8341 caaagccaga taggccttgc tgaggttcgt ggtatcgagt ttgatgtagt gctccaggct
     8401 ccagatcgcc tctttgaagc tgggcggcgt gttcttcagg tacaggtcgc ccatttcaaa
     8461 atacgcgggc gcgaaggtgg aatcggcccg cagcacctct ttgagttgct ctaaggcacc
     8521 gttgaaatca cgacccttca acagagcctg ggccaaacgg tagcgtgcct ccgacagggt
     8581 ggcatccaga accagcgctt gccggtactc atccaccgca atgggataga taccattatc
     8641 aaaattgacc tcggctaagt tcaaatggta ctcggccacc ttcggattgg cgaggatcgc
     8701 ctgccggaac gccttgtcgg cctcgtccaa tttcctctgg gcttgcaaag cgaaacccag
     8761 tccgttgaag aagcgggctt tctgggccct gtcacccgtt cccttcttga acgtctgctc
     8821 ggcttctccg ggctgaccca gcttcaggta ggtcaaaccc gacccataga gcgcatccca
     8881 gtgtttggcc ttccgcgaga gcgccgcctg gtactgcgcc agggcgtcgg aaaatttccc
     8941 caagtggtag tagcagttgc cgaggtagga gtagttttcc gggtcggccg gattcttggc
     9001 cgccagttgt ttgaataact cgacggctgc agcgtagtct ttgcgctcat aagccgccaa
     9061 ggccgcggcg gaaccgttct ggccaaaggc ctgaactgcg ccgccaacgc tgatgcccag
     9121 ccagaagaaa ccgaaaagca agcttcggat tgcccgtttt gaccagagac tttttccggg
     9181 gtctctcagc acgaacataa acggtcgtat atactggccg ccgggatttt tgtcaaacga
     9241 aaatctacgc atgccatttc ccttctgccc ggctttgcgc cgcgcgccgc gcagtgagcc
     9301 acacccgcac ccccgcgtag ctgatcaaaa gcagcccgac cagaatgcgt acgcccgact
     9361 ggtatgggaa agagcgagcg aaacccgggc tgaaaaggac gaacagcccc accccgaaaa
     9421 tcagggctaa aataagataa ctggtccaca cccacacctg acccattctc ccttccctaa
     9481 ccgtaactgg tggcgaaaca gttctctgtc ggtacgggct cgacccacct cacggacgag
     9541 ccgcaccctc cgggtggcca ctatatgcta cgtaactttt tcgcaacccg caagcgggca
     9601 tttcccccgg ctctcatttc taataacact cgctcttttc acaactccct cctctggagc
     9661 cgccagaccg aagcccccag gagtatcagg ccgaagatga aaagcgggag cacctgatcc
     9721 cacaactggc cgatcccggt ccctttgaga aaaattcggc ggacgatctc cacaaagtac
     9781 cgcaggggaa tgaggtaggt gatatactgg acggctgaag gcatattggc gatgggca
//
LOCUS       DBNGZD010000418         2855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_899563, whole genome shotgun sequence.
ACCESSION   DBNGZD010000418 DBNGZD010000000
VERSION     DBNGZD010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_899563"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC16GM_10880"
     CDS             complement(<1..448)
                     /locus_tag="AC16GM_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR2924211.1"
                     /translation="MKRIAVFDIGTNSILLLVAGKRQDGTVRVLFEAMETPRLGAGLQ
                     AAGRIGAGAVKRTIQSLKLLKKRARQYRPEKTFAIGTNVFRQAKNRKAVVRQIAEHTG
                     IEIQVISGKSEAALAYAGALSGLKGGKGNAVVLDIGGGSTELALRTS"
     gene            complement(445..1629)
                     /locus_tag="AC16GM_10885"
     CDS             complement(445..1629)
                     /locus_tag="AC16GM_10885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR2924212.1"
                     /translation="MAEVILKPKETRRLQGGHLWIFSNEIQEVRDVGENGQLVAVRGT
                     GGELLGRGYYNRHSLIAVRLLTRRQDEEIDSAFFRSRIARAIEYRRRLYPDSISGRLI
                     YSESDLLPGLIVDRYESVLVLQVTTLGLERLLPICLPVLEELLRPTGIFLRNDSSLRL
                     LEGLLQETKVLSGDVPDEMEISQHGLKFLVDVKSGQKTGFFFDQRENRERMRVKVALR
                     GKRVLDCFCYTGAWALVAAQAGAQSVIGLDESNAAIVLAQKNAALNGLADRVTFRKAE
                     VFSELRRLVSAGEKFDCVILDPPAFAKTRKDVAEAFKGYAEINRQAFKLLNPGGFLVT
                     SSCSHHLSPEDFQSVLRRAAREARVQARLVAFGGQALDHPVLLAMPETAYLKCAILQV
                     LP"
     gene            complement(1694..2683)
                     /locus_tag="AC16GM_10890"
     CDS             complement(1694..2683)
                     /locus_tag="AC16GM_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR2924213.1"
                     /translation="MPKEIWIFAEQKYGNLSRTAFELLTVAQKLAEPKSASVAAVLFG
                     HQREGLTAELFGHGAQKVYLADAPGLEKFVDESYAAVLADLIQKHQPEIVLGAATFSG
                     KALFPRLAALLQAGIITDAIAISADTQPLAVTRPAYGGNVFQELTAQGAGSLLLTCRP
                     KAFAEAPAAPGRTGEIVRHPVPEELLTSPTTVREAVKAEGQKVSLTEADVIVSGGRGL
                     REPANFKLIADLAAALGGAVGASRAVVDAGWIPYAHQVGQTGKTVNPKLYIACGVSGA
                     IQHRVGMQSARVIVAINRDKDAPIFDIATYGIVGDLFEIVPALIKKFKEKLGR"
     gene            complement(2689..>2855)
                     /gene="larC"
                     /locus_tag="AC16GM_10895"
     CDS             complement(2689..>2855)
                     /gene="larC"
                     /locus_tag="AC16GM_10895"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HIR2924214.1"
                     /translation="VQTKYGAVRLKISELDGQKRAAPEYADCARLAEQHHVPYRLIVA
                     EALKAFGLKK"
BASE COUNT          578 a          854 c          819 g          604 t
ORIGIN      
        1 cggaggttct tagcgccagt tcggtggagc caccaccgat gtcgaggacg accgcatttc
       61 cttttccccc tttcaatccg gaaagggctc ccgcataagc cagtgcggct tcgctcttcc
      121 cggaaatgac ctgaatttct attccggtgt gctccgcgat ctgccggaca accgccttcc
      181 ggtttttggc ctggcggaaa acgttcgtac caattgcgaa agttttttcc ggtcgatatt
      241 ggcgggcacg cttctttaac agcttaagac tctgaatcgt tcgcttcacc gccccggctc
      301 caatgcggcc ggcggcctgc aagccggcac ccaagcgcgg cgtttccatt gcttcaaaaa
      361 gcacccgcac ggtaccatcc tgccttttcc cggcaacgag caacaagatt gagttggtgc
      421 cgatgtcgaa aacggcgatc cgcttcacgg caacacttgc aaaatggcgc acttgagata
      481 ggcggtttcg ggcatcgcca gaagcaccgg atggtcgagc gcctggccgc cgaaggccac
      541 gaggcgtgcc tgcactctgg cttcccgcgc ggcgcggcgc aatacactct ggaaatcttc
      601 cggcgaaagg tggtgggaac agctggatgt caccaaaaat cctcccgggt tgagcagttt
      661 gaaggcctgg cggttaattt ccgcataccc tttgaaggcc tcggcgacat cttttcgggt
      721 tttggcaaag gcgggcggat cgagaatgac acagtcgaac ttttcaccgg cgcttaccag
      781 acggcgcaat tcgctgaaga cttcggcttt ccggaaagta acacggtcag ccaggccatt
      841 taacgccgcg ttcttctggg ccagcacaat ggcggcattc gattcatcca gtccgataac
      901 cgactgcgcg cccgcttgag cggccaccag tgcccaggcc ccggtgtagc agaagcagtc
      961 gagtacccgt ttacccctca gggccacttt cacccgcatt ctttcgcgat tctcgcgctg
     1021 atcgaagaaa aatccggtct tctgacccga ttttacgtca acgagaaact tcaagccgtg
     1081 ctggctgatt tccatttcgt ccggcacgtc accggagaga accttcgttt cctgcagcaa
     1141 gccttctaac aggcgcaagg aagaatcgtt gcgaaggaaa ataccggttg gacggagcaa
     1201 ttcttccagc accggcaggc agatcggcaa aagccgctcc agtcccagtg tggtcacttg
     1261 cagtacgaga accgattcat accggtctac gatcaggccg gggaggagat cgctttcact
     1321 gtaaatcagg cgtccggaaa ttgagtcggg gtacaatctc cttcgatact caattgccct
     1381 ggcgatgcgc gagcggaaaa aggcactgtc aatttcctca tcctgcctgc gcgtcaacaa
     1441 acgcacggcg atcaaactgt gccggttgta gtacccgcgc cccagtagtt caccgccggt
     1501 gccgcgcacc gccacgagtt ggccgttctc tccaacatct cgcacctctt gaatttcgtt
     1561 ggagaaaatc cagagatggc cgccctgcag gcggcgcgtt tctttcggtt tgaggatgac
     1621 ttctgccatt ggggaggagg aagagggctg atctgatagt aaggccggag gggagacggg
     1681 gacgtgcgta ccgctaccgc cccaatttct ctttgaactt cttgatcaga gcggggacga
     1741 tttcaaacag atcccccacg attccgtaag tggcgatgtc aaaaatcggc gcatccttgt
     1801 cgcggttgat ggcgacgatg acccgggccg actgcatccc gacccggtgc tggatggcgc
     1861 ccgaaacgcc gcaggcgatg tagagtttgg ggttgaccgt tttgccggtc tggcccacct
     1921 ggtgggcgta gggaatccag ccggcatcca ccaccgcgcg agaagcaccg accgcgccgc
     1981 ccagggcggc ggccagatcg gcgatcaatt tgaaattcgc cggctcgcgc aagccccggc
     2041 cgccggagac gataacgtcg gcctcggtca aactgacctt ctggccttcg gctttgaccg
     2101 cttcgcgtac cgtcgtcggc gaggtcaata gttcttccgg aacagggtgc cgaacgattt
     2161 cacccgtgcg gccgggtgcg gcgggtgctt cggcaaaagc tttgggccgg caggttaaga
     2221 ggagtgagcc tgcgccctga gccgtcagtt cttggaaaac attgccgccg taagcggggc
     2281 gcgtcaccgc cagtggctga gtgtcggcgg aaatggcgat ggcgtcggta attatgcccg
     2341 cctgcaaaag cgccgccagg cgggggaaaa gcgctttacc cgaaaaggtg gccgctccca
     2401 gcacgatctc cggctggtgc ttttgaatca ggtcggccag caccgccgcg tagctttcat
     2461 ccacgaattt ttcgaggccg ggggcgtcgg ccagatagac cttctgtgct ccatggccga
     2521 acagctccgc agttaaccct tccctttggt gaccgaacaa aacagccgcg accgaagcac
     2581 ttttcggctc ggctaatttt tgggcgacgg tcaatagttc gaaagcggtg cgagagagat
     2641 tcccatattt ctgctccgcg aaaatccaga tttctttggg catagcctct actttttcag
     2701 gccaaaggct ttcaaggcct cagcgacgat cagccggtac ggcacatggt gctgttcggc
     2761 taagcgcgcg caatcggcat actcgggcgc ggcgcgcttc tgcccgtcca attcacttat
     2821 tttcaaccgt accgctccgt atttggtctg gacca
//
LOCUS       DBNGZD010000419         4336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1103169, whole genome shotgun sequence.
ACCESSION   DBNGZD010000419 DBNGZD010000000
VERSION     DBNGZD010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1103169"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC16GM_10900"
     CDS             <1..243
                     /locus_tag="AC16GM_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924215.1"
                     /translation="NFLDVPHNIDRGFIDPWKIPYLPRYHIAQKIGDAMSFVYKKLKV
                     TPNSPSFSKISIRVYKDWYSFVRQVHLNFLSAIRNL"
     gene            complement(309..1286)
                     /locus_tag="AC16GM_10905"
     CDS             complement(309..1286)
                     /locus_tag="AC16GM_10905"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR2924216.1"
                     /translation="MSLPESEAKILDAYSQAVTKVADTVGPAVVNITTSRSPKPYWFE
                     PVPNERAGIGSGVIVRAEGYIVTNAHVVSRSPGLTVTLTDGRRFPARLIGRDRRFDLA
                     VLKIDATRLHAAQLGDSHQLKVGQLVVAIGNPFGLEWTVTAGVVSSLGRTLRLPGGFV
                     AENVIQTDASINPGNSGGALCDSTGKVIGITTAVIYGAQGIGFAIPGETVREVFHEIV
                     EGRTIQKAWLGISGFNQALESEVVAAFNLPSRTGVLVVEVVPNSPADRGGIQQFDIIV
                     AVGGTQTTTVRALQEALRQTVISEPVPVTILRGHQWLQVSVKPEVSPVG"
     gene            complement(1366..1857)
                     /locus_tag="AC16GM_10910"
     CDS             complement(1366..1857)
                     /locus_tag="AC16GM_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924217.1"
                     /translation="MTKKLLSFVVIGMFLLALASFATAGDKPAGKMMGSPNCPACAEG
                     MACEKALKGMGAAMEWVDLSNGLACITTAASPSKATAVQTHMQKHGQKMDELMKTQRD
                     KFCDMCKQMSALIEAGNQQVVNFKGGSMWMVTSNDPKTVESLHQMAAECRSMGTQSAE
                     KKL"
     gene            2216..3808
                     /locus_tag="AC16GM_10915"
     CDS             2216..3808
                     /locus_tag="AC16GM_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR2924218.1"
                     /translation="MRNRSPKPSPPPTTPGAELRRDVGVWGSFMWGYSDVGADIYVAL
                     GLVMAYAQGAASLVFALAGSVYIMIGLAYTELASAYPVAGGGPYFTLRGLGDFWGFVG
                     GSALLLDYTIDIALFATASAGYLNFFLPQLIGRSIQSFAVTLGPFHNVNPVWCLETLL
                     LIAILILLNIRGVRESTFLQSFIGIVDLLAEGSIIIFGFLFAWKPELLALQWKTQFPS
                     FHNLVYGSSLAIISFVGLESISQAAQETKRPATVIPRTSLTLIFTVFIFAVSFSTLGL
                     GVFPWQVFRDHMGDPVAYLAHGIPFIGFIAGPFAAILGGTILFISANSGIFGASRLTY
                     SLAQFGSLSPWFCSVHPKFHTPVRTIVIFSMIGVVQTILAFLTPSAMDTLGNMYAFGA
                     ATGYTLVFIALIKLRFSDPYTPRPYRVPLNFKLRYKGRRVDFPVLGVIGTLGIATILF
                     EVVLTHAIGRIAGPAWVLLCFVYYAWYRKKAGFPVFRSIRHDWEREQIDVLTSAEEFD
                     LLEQYKNALKEKRKEPLHVPAD"
     gene            3792..4025
                     /locus_tag="AC16GM_10920"
     CDS             3792..4025
                     /locus_tag="AC16GM_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924219.1"
                     /translation="MSRLTEKFSRLSPGDKYRFFTTGSFFVLGAVIAVRGLAVGIFPV
                     LMGIAFVGFGIYRFYYFYRYFRQGRSKAPHAVR"
     gene            4012..>4336
                     /locus_tag="AC16GM_10925"
     CDS             4012..>4336
                     /locus_tag="AC16GM_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2924220.1"
                     /translation="MLSVEITFLGAFLAACFAVAMGGLLWWMLHPPEAVKLLVARAYR
                     SVDALKRILVPTTGLPYSDRGVELACRLGEMQKADIILTYVIEVPRTLPLNAPLPEAE
                     TKAVEA"
BASE COUNT          828 a         1257 c         1162 g         1089 t
ORIGIN      
        1 aattttttgg atgtgccaca taatattgat cgaggtttta ttgacccgtg gaaaattcct
       61 tatttgcccc gttatcatat cgcgcagaag atcggcgatg caatgtcctt tgtatacaag
      121 aagctaaagg tcacacccaa tagcccatct ttttcaaaaa tctccatccg tgtttataaa
      181 gactggtatt ctttcgttcg acaagttcat ctaaattttt tgtccgctat ccgaaatctt
      241 tgatctctcc cactgccttc ggcgagagtc tatcgaccct caaggggagg taaaccaggt
      301 acagaatctc atcccaccgg agagacttcc ggtttgacgc tcacctgcag ccactggtgc
      361 ccgcggagaa ttgttaccgg tactggttcg ctgattacgg tctggcgcag ggcttcctgt
      421 aaagctcgca cggtagttgt ctgcgtgccg ccgacggcga cgatgatgtc gaattgctgg
      481 atgccgcccc ggtcagccgg gctgttcggt accacttcca ccaccagaac gccggtgcgg
      541 gaaggcaggt tgaacgccgc caccacctcc gattccagcg cctggttaaa tccgctgatg
      601 cccagccagg ccttctgaat cgtcctgcct tccacgattt cgtggaacac ttcgcgcacc
      661 gtttctccgg ggatggcaaa accgattccc tgcgcgccgt aaatcaccgc cgtcgtaatc
      721 ccgatgactt taccggtaga gtcgcacaag gccccgccgg agttgccggg attgatggag
      781 gcatcggtct ggatcacatt ttcggccaca aagccgccgg gcaaacgcag tgtccgtccc
      841 agagaactga cgacgccggc ggtgaccgtc cactccaatc caaacggatt accgatggcc
      901 acaactaact ggccgacttt taactgatgc gaatcgccca actgcgcggc gtggaggcgg
      961 gtcgcatcta ttttaaggac tgccagatcg aaccgccggt cgcggccgat aagccgggcc
     1021 ggaaaccggc ggccgtcggt cagggtaaca gttaagccgg gactgcggct gactacgtgg
     1081 gcattggtaa ctatgtaccc ctcggcgcgg actatgacgc cggagccgat accggcgcgc
     1141 tcgttcggca cgggctcaaa ccagtaaggt tttggggagc gtgaggtggt gatgttgacc
     1201 accgcggggc cgaccgtatc ggccactttc gtcacggctt gggagtaggc gtctaagatt
     1261 tttgcttccg attcggggag cgacatagaa ccctttctta agcaagaaaa accgtggcag
     1321 agagtgtacc gccccggccc acacactgtc ccgccattgg cgggattaca gcttcttctc
     1381 tgcggactgc gttcccatag agcgacactc ggccgccatc tggtgcagcg attccacggt
     1441 cttcgggtcg ttcgaggtga ccatccacat cgagccaccc ttgaagttga ccacttgctg
     1501 gttgcctgcc tcgatcaagg cagacatctg cttgcacata tcgcagaact tgtccctctg
     1561 ggtcttcatc agttcatcca tcttctgccc atgtttctgc atatgtgtct ggaccgccgt
     1621 cgctttggag ggcgaggccg cggtcgtgat gcaggccaag ccgttggaga ggtccaccca
     1681 ttccatggcg gcgcccatgc ccttcagggc cttctcgcag gccatccctt ccgcacaggc
     1741 tggacagttc ggtgagccca tcatcttccc ggccggctta tccccggcgg tggcaaagga
     1801 cgccagagcc agcaagaaca tcccgatgac gacgaatgac aatagcttct tggtcatttc
     1861 ctgttttcac ctccttccgt agagttaagg ttttggagtt tatttcccgg tttcatttca
     1921 gaccaaattt ctcttgatag acggagtaag cagagtattt gttagacttt tcttttcaga
     1981 tgtctccaac cgcccggatg ctaatacttt ttctctgaaa aagcaagagc aattggcggc
     2041 gggcgggtgc atttgacatt cctccctgcc aatctatatt cccccgccgt tctatcttcg
     2101 cgcggcatat ggattggcgc cccacgaggg gtgtggcctg cgcggcgatg cgtatgaaaa
     2161 agccaaaaat gaggcaaccg cagaccaggt gggcactggt cttttttgtt agcctatgag
     2221 aaacagaagc cctaaaccgt ctccgccacc caccacaccg ggagccgaac tccggcgcga
     2281 cgtcggcgtt tggggctcgt tcatgtgggg ctattcggat gtcggggcgg atatttatgt
     2341 cgctctgggc ttggtcatgg cctatgccca gggagccgcc agcttggtct ttgccttagc
     2401 cggatcggtc tacatcatga tcggcctggc ttacaccgag ctggcgtcgg cctaccccgt
     2461 cgccggtggg gggccatact tcactctgag agggttaggc gacttctggg gctttgtggg
     2521 cggttccgct cttctcttgg attacacgat tgacatcgcc ctctttgcga ctgcttcagc
     2581 cggctacctg aacttctttc tgccgcagct aatcggccgc agcatccaga gcttcgccgt
     2641 cactctcggg ccgtttcaca acgtcaatcc cgtgtggtgc ctggaaactt tacttttaat
     2701 tgcgattttg attctcttaa acataagggg agtccgggag tcaactttcc tgcagtcttt
     2761 tatcggcatc gtagaccttc tggctgaggg cagcatcata atcttcgggt ttctctttgc
     2821 ttggaaaccg gaactgctcg ccctgcagtg gaaaacccaa ttcccctcct ttcacaacct
     2881 tgtctacggt tcctccttgg caatcatttc ctttgtaggg ctggaatcga tctctcaggc
     2941 ggcccaggaa accaaacgtc cggccaccgt cataccgcga acttcactca ccttgatatt
     3001 caccgttttc atctttgcgg tatcgttttc cactctgggt ctgggtgttt tcccttggca
     3061 ggtctttcgg gatcacatgg gcgacccggt ggcatattta gcgcacggga ttcctttcat
     3121 cggctttatc gccgggccct tcgccgcaat tttggggggc acgattttat tcatctccgc
     3181 caacagcggg atattcggcg cctcccggct gacctattct ctggcccagt tcggcagcct
     3241 cagcccttgg ttttgcagcg tgcatccgaa atttcacacc ccggtcagaa ccatcgtcat
     3301 tttttcgatg atcggtgtgg ttcaaacaat cttggccttc ctcacccctt cggccatgga
     3361 tacgcttggc aatatgtacg ccttcggtgc cgccaccggg tatacgctgg tcttcatcgc
     3421 cttgatcaag ctccgtttca gcgatcccta tacacccaga ccgtatcgag ttcctctcaa
     3481 ttttaaactt cgctacaagg gcagaagggt agattttccc gtcctgggag tcattggaac
     3541 tttggggatt gcgaccattc tttttgaggt cgtcctgaca catgccatag gcagaatagc
     3601 cgggcccgcc tgggtgctct tgtgcttcgt ctactatgcc tggtaccgga agaaggcggg
     3661 attccccgtt tttcggagca tccgccacga ctgggagagg gagcaaatcg acgtgctcac
     3721 ctcagccgaa gagtttgacc tgctggagca gtacaagaac gccctcaaag aaaaaaggaa
     3781 ggaaccgttg catgtcccgg ctgactgaaa aattttcccg actctcgccg ggggacaagt
     3841 accggttttt caccaccggt tccttttttg tcctgggcgc ggtcattgcg gtccggggtc
     3901 tggcggttgg tattttccca gtccttatgg gaattgcttt cgtaggattt ggcatctacc
     3961 ggttctacta cttctaccgc tatttccggc agggccggtc aaaagcgccg catgctgtcc
     4021 gttgagataa catttttggg cgcttttctg gccgcctgtt ttgcagtcgc catgggcggg
     4081 ctgctctggt ggatgctcca cccgccggag gcggtcaagc ttctggtggc gcgggcctac
     4141 cgctcggtgg atgcgctcaa gcggatttta gtccccacca ccggtttacc ctattccgac
     4201 cggggggtgg aactcgcctg ccgcttgggg gaaatgcaga aagccgatat tatcttgacc
     4261 tacgtgattg aagtgccgcg gactttgccc ttgaacgcgc ctttgccgga ggcggaaacc
     4321 aaagcggtag aggcct
//
LOCUS       DBNGZD010000420         1452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_553585, whole genome shotgun sequence.
ACCESSION   DBNGZD010000420 DBNGZD010000000
VERSION     DBNGZD010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_553585"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="AC16GM_10930"
     CDS             <1..1034
                     /locus_tag="AC16GM_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924221.1"
                     /translation="FWDQFTPLLLVVVLGGWVALWGKSRTWWFFSSLLLLGNALMAAV
                     LSDEIILDNPDLHGYLQPSILTLGLWFGVGIFAALALVDRTAARWHARPDFRWLLSAV
                     FSGFFFLIGFLPWWSNRSYADLSANRLAERVGLSVLEPLPEHAVAFIDNPNLDFILRG
                     LQIAENKRPDVIVVDRALLGGSWYVRQTRRNFPFLFAGVPEHLAGESLFVILRDGCLE
                     QGIPAFMEFTEHDISFVHHLMPSGYLYQIRRDSVGEIPKEVMLYQKEWARKQFINDDD
                     VSRLHDVDGIRMLVLTWFRTGLFFEAKGLRPQAQAFYRKTRRAARPEKALPDHPQNPA
                     DNADKIPKP"
     gene            1161..>1452
                     /locus_tag="AC16GM_10935"
     CDS             1161..>1452
                     /locus_tag="AC16GM_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR2924222.1"
                     /translation="MSKGFTLIELLIVVIIIGVLAAAAIPSFLRASTKSKQAEAKQFL
                     KEIYTMELAYRHSQDTYIAYGGVRGGGSLPEIGVEIPTSAKYIYSVTAGPTGI"
BASE COUNT          266 a          416 c          434 g          336 t
ORIGIN      
        1 ttttctggga ccaattcacg cccttactat tggtggtcgt tttggggggc tgggtggcgc
       61 tctggggaaa gagccgtacc tggtggttct tttcgagttt gctccttctg ggcaacgcgc
      121 ttatggcggc ggttctctcc gatgaaatta ttctggataa tcccgacctg cacggctacc
      181 tccagccgtc gatcttgacc ttggggttgt ggtttggcgt cggcattttt gccgcgctcg
      241 cactggtaga ccgcacggcg gcacgctggc acgcccggcc cgatttccgc tggcttttgt
      301 ccgccgtctt cagcggcttt ttctttttga tcgggtttct gccgtggtgg tcaaaccgga
      361 gttacgccga tctgtcggcc aaccgcctgg ccgaacgggt gggtctttcg gttttggagc
      421 cgctgccgga acacgcagtc gctttcatcg acaatccaaa tctagacttc atcctgcgcg
      481 gcctccagat cgccgaaaac aaacggccag acgtcattgt agttgaccgc gcccttttgg
      541 gcggcagctg gtacgtccgt cagacccgcc gcaattttcc tttcctcttc gctggtgtgc
      601 cggagcactt ggcgggagaa agtctgttcg tcatcctgcg cgacggttgc ctggagcagg
      661 gtattcccgc gttcatggaa ttcaccgaac acgacatctc cttcgtgcac cacttgatgc
      721 cctccggcta cctgtaccaa atccggcgcg actcggtcgg tgaaattccc aaagaggtga
      781 tgctgtacca gaaggagtgg gccaggaaac agttcattaa cgacgacgac gtttcccggc
      841 tgcatgacgt ggacggcatc cggatgttag tgttgacgtg gttccggacg ggtcttttct
      901 tcgaagccaa aggcctccgg ccgcaggcgc aggccttcta ccgtaaaacc cggcgggcgg
      961 cccgtccgga aaaggcgctc cccgaccatc cccaaaatcc ggcggacaat gccgataaaa
     1021 taccaaagcc gtagcggcac aacggaaact cctatggaga aaatagcgct ggcgttagcc
     1081 atggcgtttg ccgatccggc tgggccggag cagttgtggg acctcctgag acgaggcggg
     1141 cgatgaccgg tggccgacgt gtgtcaaaag gctttacgct cattgagctt ttgattgtcg
     1201 tgattatcat cggcgttttg gccgccgcgg cgatcccgag cttcctgcgg gcttcgacca
     1261 aaagcaagca ggcggaagcg aaacagttct taaaggagat ttacaccatg gagctggcct
     1321 accggcacag ccaggacacc tacattgcct acgggggtgt gcgaggaggc ggctctctgc
     1381 cggagatcgg cgtggagatt cctacctcgg cgaagtacat ctattcggtt actgccggtc
     1441 ctacgggaat tg
//
LOCUS       DBNGZD010000421         7028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1663654, whole genome shotgun sequence.
ACCESSION   DBNGZD010000421 DBNGZD010000000
VERSION     DBNGZD010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1663654"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3213)
                     /gene="porU"
                     /locus_tag="AC16GM_10940"
     CDS             complement(<1..3213)
                     /gene="porU"
                     /locus_tag="AC16GM_10940"
                     /inference="COORDINATES: protein motif:HMM:NF033707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HIR2924223.1"
                     /translation="MSRRSQTLLILAGLLWMVSVPAGAKALPSDVTLLSADDRGVVLT
                     YAVPQTEITPIETPNGKFQRIYLPNCPNSTFPGEPELPARIILLGVPPEAEITAEVVT
                     FDFDERAGIDVCPVPGVSGGDQKLGTPIAKYQKSQSAYALAGFFPSGVVAVEGPFFLR
                     HQRAVRVKLFPVQYNPQTRTLRVARQITVRVNFSRGGTTPSQAALPSGDSYEPIYRAG
                     LLNYEQARGWRVTPPPQSLAQAAQVDSFSRSPDWYKITVRDNAIYKIDAPLLAAAGVN
                     LATLDPRTIRIFSGDGRALSPFNVDPRPQFQELAILVTGDVDGRFDPSDLILFYGWSV
                     DRWTKTAAGQDTFVRHPYTRENVFWFTFGGSFPTPPKRMATRDGSLRFAAPSVPTDFT
                     ELAHFEDDRIFGQTSGGDVVDYFTWYWLNNQKPPPLFFNLPGVAAAAPETVAITHTNT
                     GMNLTVNGVLTTALGPGNGISRFVTSSLVDGLNQFALEFFTNPFDPRYYLNAIEMAYP
                     RRFQAVSDFLPFGFTGTGAHRFDVSGITSTAALLLDVTNGFDQVQMINVPPPQGGVFR
                     FADSLSGTEIRYAVAGSAAWRRPLRLAREVPSNLRDPASRADLLVITHDDFVAEANQY
                     AAYRQAQSGLTTKVVKVSDIYDQFAWGLFDPVAIRDFLKYTFENWTQPAPSFVLLVGD
                     GSYDYRNNLGTGAKNYIPPFIAAPAFDASTVADENYLYFGQPGILDPPPFDQVLAMVI
                     GRWPVRTPEEVQTVIAKVKAYEATPELGEWRNQITLVADDEFGEPPFGVAEAFHTQQT
                     EGLARHVPPLFSLNKIYLFEFPFDANRKKPTAEEAIISTWNRGTLIIDYIGHGNPDVW
                     AHESVFKRGQDIPRLTNFTRLPLVYNASCSIGFFDSPFSEGMGEELVRSPLGGAIAVI
                     AATRLAFAFQNVALNEQGFDFLLGQDSLSIGQALYLAKLLRQYQLCFPSPPCPQPNDR
                     QYVLFCDPALHLGAPKNRVRFLPPVPDSLAALTVAQIIGEATDTSGAPLPGFSGSADV
                     VVFDAERPRAYIMAALPNSPVTYTLPGNRIYRGKAQVNGGQ"
     gene            complement(3737..4687)
                     /locus_tag="AC16GM_10945"
     CDS             complement(3737..4687)
                     /locus_tag="AC16GM_10945"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR2924224.1"
                     /translation="MTPRVKEILGWYGSENPGVRANLARIMNHGTLAGTGRFVILPVD
                     QGFEHGPARSFAPNPAGYDPRYHFQLAIEASCNAYAAPLGFISDGAADYAGEIPLILK
                     LNNADTLSKAGDPVQAVTGSVRDALRLGCAAVGFTIYPGSAYAREMYQQVRELAEEAK
                     SFGLAVVVWSYPRGSGLSKEGETAIDVVAYAAHIAAQLGANIIKVKPPTAHIEQAEAK
                     KVYETQRIPIGTLTERVRHVVQSTFNGRRIVIFSGGPAKTDAELLEEVRAIKAGGGFG
                     SIIGRNSFQRPKPEALELLRKIMALYKDEPVRQLVQTALV"
     gene            complement(4741..6144)
                     /locus_tag="AC16GM_10950"
     CDS             complement(4741..6144)
                     /locus_tag="AC16GM_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR2924225.1"
                     /translation="MLIEPYKIKVVEPIRLISRETREARLREAYFNLFNLKAEDVYID
                     LLTDSGTAAMSDAQWAGLMQGDESYAGAKSFYRFEETVRKIFGYRHVIPVHQGRGAEH
                     VFFRALLKPGDIVLANSHFDTTRANVEHRGAIAQDCLVAEGWDVDSDYPFKGNIDLAQ
                     VEETIRTHGKEKIPLCLLTITNNAGGGQPVSLENMRSLRALLKPHGIPLFFDAARFAE
                     NAYFIKTREAGYRDKSIPEICREMFACADGCLMSAKKDGLANIGAFAAVNDDDLAQGI
                     KNNLVLFEGFPTYGGLAGRDLEAIARGLEEVLDEGYLSFRVGQVAFLGRRLSEMGVPI
                     LKPVGGHAVFVDAGRFLPHIPRDEFPGQALVVALYREGGIRAVEIGSLSFGGASPVSG
                     EKHRVIAELVRLAIPRRVYTNAHLDYVVATIETIARKRDSLRGFAITYEPQVLRHFSV
                     KLMEINEKTVRKTAVGV"
     gene            complement(6163..>7028)
                     /locus_tag="AC16GM_10955"
     CDS             complement(6163..>7028)
                     /locus_tag="AC16GM_10955"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR2924226.1"
                     /translation="GGIERFGLTSQELAIICGSHSGQPEHIQVVEQLLMKVGFIEGDL
                     QCGIHPPSFFTANELTPEPGARFSQLHHNCSGKHTSMLALSKIYDEPKENYLDPNSRA
                     QQAILQAVAEACRYPAVEIGVAIDGCSAPNFALPLRHMARGYANFVTAHGSTPASSRV
                     YQTLRQAMLEHPLMVSGVGRFDYALMQAGRGQVLSKAGAEALECAALVDRGWGLAVKI
                     ADGSQRAVAPVVIEVLSQLGVPLDMSVPKLAEFARPVIKNYRGRVVGQIAPIFKLQAV
                     KPARVREAVAV"
BASE COUNT         1507 a         2066 c         2016 g         1439 t
ORIGIN      
        1 ttgaccgccg ttcacctgcg ctttgccccg ataaatccgg ttgcccggga gtgtgtaagt
       61 caccggggag ttgggcaagg cggccataat gtaggcccgc ggtctttccg catcgaaaac
      121 caccacgtcg gctgaacccg aaaagccggg aagcggggcg ccactcgtat ctgtcgcttc
      181 gcctataatc tgcgccaccg tcagggcggc cagagaatcg ggcaccggtg ggaggaaccg
      241 cacccggttt ttcggcgcgc ctaaatggag tgccgggtca cagaacaaaa cgtactgccg
      301 atcatttggc tgcgggcagg gcggcgaagg aaaacacaac tgatactgcc gcagtaactt
      361 cgctaagtag agtgcctgac cgatcgaaag cgagtcctgt cctaagagaa aatcaaaccc
      421 ctgctcgttc aacgcgacgt tctggaacgc aaaggccagc cgggtggcgg cgatgacggc
      481 aatggcgccg cccagaggtg agcggaccag ttcctcaccc attccctcgg agaaagggga
      541 gtcgaaaaaa ccgatactgc acgaggcgtt atagaccaga ggcagacggg tgaaattggt
      601 caagcgcgga atatcctgcc cgcgcttgaa gaccgattcg tgcgcccaga catccgggtt
      661 gccgtgcccg atgtagtcga taatcaaagt tcctctgttc caggtgctga taatagcttc
      721 ttccgccgtc ggcttcttgc ggttcgcgtc gaagggaaac tcaaagaggt agattttgtt
      781 cagagagaaa aggggcggca cgtgacgtgc gagaccttca gtctgctggg tgtggaatgc
      841 ttccgccacg ccgaaaggcg gctcgccgaa ttcgtcatcg gccaccaggg tgatctggtt
      901 gcgccactct cccaactccg gggtggcctc atacgctttt actttcgcga tgaccgtttg
      961 cacttcctcc ggcgtccgca ccggccagcg gccaatgacc atcgccaaca cctgatcgaa
     1021 cggcgggggg tctaaaatcc ccggctggcc gaagtagagg tagttctcgt cggctacggt
     1081 gctggcgtca aaggcgggcg cggctatgaa gggtggaatg taattcttgg ccccagtccc
     1141 taagttattg cggtagtcgt aactgccgtc gccgaccaac agcacaaaac tcggtgcggg
     1201 ctgggtccaa ttctcaaagg tgtatttgag aaaatcgcgg attgccaccg gatcgaaaag
     1261 tccccaggcg aattggtcgt aaatatcgga cacttttacg accttcgtgg tcaagccgga
     1321 ctgcgcctgc cggtaagccg catattgatt cgcctcggcc acaaagtcgt cgtgcgtgat
     1381 caccagcaag tctgctcggc tggcggggtc acgcagattc gatggcactt cccgggctaa
     1441 acgcaaaggt cggcgccagg ccgcactgcc ggccactgcg tagcgaatct ctgttccgga
     1501 cagactgtct gcgaaccgga agaccccgcc ttggggaggt ggcacgttaa tcatctgcac
     1561 ctggtcaaat ccgttggtga catccaaaag taacgctgca gtggaagtga tcccgcttac
     1621 gtcgaaccta tgagcgccag taccggtgaa accgaagggc aggaagtcgc tcaccgcttg
     1681 aaagcggcgg ggataggcca tttcaatagc attgagatag tagcgtgggt caaacgggtt
     1741 agtaaaaaat tccagggcaa actgattgag gccatccacc agactggatg ttacgaaccg
     1801 gctgatccca tttcccggac ccagtgcggt cgtcagcaca ccgttcacgg tcaggttcat
     1861 cccggtattg gtgtgggtga tggcgacggt ttccggtgcc gccgcagcca cgccgggaag
     1921 attaaaaaat aagggaggcg gcttctgatt gttcagccag taccaggtga aataatcgac
     1981 aacgtcgccg ccggaggttt gcccgaagat ccggtcatct tcaaagtgcg ccagttcagt
     2041 gaaatcggtc ggcacgctgg gagcggcaaa acgcaacgaa ccgtcacgcg tcgccatccg
     2101 tttgggcggg gttgggaaac tcccgccaaa ggtaaaccag aagacattct ctctggtgta
     2161 ggggtggcgg acgaaagtat cctgcccggc ggcagttttc gtccagcgat cgaccgacca
     2221 accgtaaaag agaatcaaat ctgacggatc aaagcggcca tcgacatcgc cggtaaccag
     2281 aatagccaat tcctggaatt gcggccgggg atcaacgttg aagggcgaga gtgcacggcc
     2341 atcccccgaa aagatgcgga tggtgcgcgg gtcaagcgtt gcgaggttta cccccgcggc
     2401 cgcgagcagc ggcgcatcga ttttgtagat cgcgttgtcg cgcacagtga ttttgtacca
     2461 gtcgggcgac cgggaaaaag aatcaacctg agccgcttgg gccagggatt gaggcggcgg
     2521 cgtcacacgc caacctctgg cttgttcgta attcaacagc cccgctcggt aaataggttc
     2581 atatgaatca ccagacggaa gagccgcctg agaaggggtg gtgccgccac gagagaagtt
     2641 aacgcgcacg gtgatctgcc gggccacgcg cagggtgcgt gtttggggat tgtactgcac
     2701 cggaaaaagt tttacccgga ccgcccgctg gtgacgcaga aaaaaaggac cttcaacggc
     2761 cactacgccg gagggaaaaa acccggccag agcatacgca ctttgtgact tctgatattt
     2821 ggcaatgggt gtgcccagtt tttgatcgcc gccactcact cccggaaccg gacagacatc
     2881 aatgcccgcc cgttcgtcaa agtcaaaagt gaccacttca gccgttatct ccgcttccgg
     2941 aggaacgccc aaaaggatga ttcgggccgg cagttccggc tcgccgggaa aagtcgagtt
     3001 cgggcaattg gggaggtaaa tcctctggaa cttgccattc ggggtttcta tgggagtaat
     3061 ctccgtctgc ggcacggcgt aggtgaggac cactccccgg tcatcggcgg agagcaaagt
     3121 gacgtccgaa ggcaatgcct ttgccccggc tggcaccgat accatccaga ggaggcctgc
     3181 caggatcagt aacgtttgcg agcgtcgcga cataaataaa aaaaccgagc cacaggttgt
     3241 accacacccg tcttgcccgg ttcgttaccg gctggtgctt tcgttttgcc gacttaccct
     3301 tcgacagata aagggctaag tctgcgcgtg atacaacctg ttaactctgg ctcgttattt
     3361 tcgctcctgg tagtctgtgg ctaacttaaa gcaaatatag gctctacggt caaaactgtc
     3421 aagggagaaa tcgcaatccc cgctcatttt ggctctttgc tgacccgttg caaactgcct
     3481 gccagcgcaa gtcactttat cttctggtaa ctgtttgcct gactgcggct tgctccaatt
     3541 ttttctctcc gccgactact ctctattcct gattgcgctg ccgtctgacc aatggttgca
     3601 agaacttgcc gggaattcca ccgcggtgaa ttgaccgaga cacagtcagc tggcatgttc
     3661 tttatcggca ataaaaacga caaaatcaaa cgccgggggt cctcaccagt caagggttgt
     3721 aaaattacgg ggcgatttac accaatgccg tttgtaccag ctgacgtact ggttcgtctt
     3781 tgtagagcgc catgatcttg cgcaaaagtt ccagggcttc cggcttgggg cgctgaaaac
     3841 tgttccggcc gatgatggag ccgaatcccc cgccagcctt gatcgcccga acctcctcca
     3901 aaagttccgc atcggtcttg gccggccccc cggagaagat gacgatcctg cggccgttga
     3961 aagtgctttg caccacgtgc cgcactcgct cagtcaaagt cccgatcgga atccgctggg
     4021 tctcgtagac cttcttggct tcggcctgct cgatgtgagc cgtcgggggt ttgaccttga
     4081 tgatattggc acccaactgc gccgcaatgt gcgcggcata ggccaccacg tcaatggcgg
     4141 tctccccctc tttagagagt cccgaaccgc gggggtagga ccaaaccacc accgccagac
     4201 caaaggactt ggcctcttcg gccaactccc gcacctgctg gtacatctcc cgggcgtagg
     4261 cggagccggg gtaaatcgta aagccaacag ccgcgcaccc cagccggagc gcatcccgca
     4321 ccgagccggt tacggcttgc accgggtccc cagccttact gagtgtgtct gcattattca
     4381 acttgagaat caggggaatt tcgcccgcat agtcagccgc accatcgctg ataaagccca
     4441 agggcgccgc gtaggcattg caactggcct cgatggctaa ctggaagtgg tagcgcgggt
     4501 catacccggc gggattcggg gcgaaactcc gcgccggacc gtgctcaaaa ccttgatcga
     4561 cggggagaat gacgaatcgc ccggttccag ccaacgtccc gtggttcata atacgcgcca
     4621 gattggcccg cacgcccggg ttttccgagc cataccagcc caatatttct ttaactctcg
     4681 gtgtcatatc tcttctcctg aaacgtacgc tgcacccgga agttcactcc caccttcgac
     4741 ctacacccct accgcggttt ttcgtacagt tttctcattg atttccatca gcttcaccga
     4801 gaaatgccgc agcacctgcg gctcgtaggt gatggcgaaa cctcgaagtg agtctctttt
     4861 ccgggcaata gtctcgatgg tcgcaacgac gtaatcgaga tgggcattcg tgtagacccg
     4921 gcggggaatt gccaggcgga ctaattcggc gatgacccgg tgcttttccc cgctcaccgg
     4981 cgaggcaccg ccgaaggaga gcgacccgat ctcgacggcg cggatgccgc cttcgcggta
     5041 gagcgccact accagcgcct gccccggaaa ctcgtcacgg gggatgtgcg gcaaaaagcg
     5101 ccccgcatcg acgaacacgg catggccgcc caccggcttg aggatcggca cacccatctc
     5161 cgagagccgg cggccgagaa aggccacctg cccgacgcgg aatgacagat agccctcatc
     5221 caagacttct tccagcccgc gcgctatggc ttcgagatcc cggcctgcca gaccgccgta
     5281 ggtggggaag ccttcgaaga gcaccaggtt gtttttgatc ccctgcgcca gatcatcatc
     5341 gttgactgct gcgaacgccc cgatattcgc caggccgtct tttttggccg acatcaaaca
     5401 gccgtcggcg caggcgaaca tctcccggca gatttccgga atcgatttgt cccggtagcc
     5461 cgcctcgcgg gtcttgatga agtaggcgtt ctcggcaaag cgcgccgcat caaagaaaag
     5521 cggaatgccg tggggtttca ggagtgccct gagcgagcgc atattctcca gtgataccgg
     5581 ctgcccgccg ccggcattgt tggtgatggt gagcaagcag agcggaattt tctctttccc
     5641 gtgggtccgg atggtctctt ccacctgcgc caggtcaatg tttcccttga agggatagtc
     5701 ggaatcgaca tcccagccct cggccaccag acaatcctgc gcgatggcgc cgcggtgttc
     5761 gacattggcc cgcgtcgtgt cgaagtgact attggccaaa acgatgtcgc ccggtttcag
     5821 gagggcccgg aagaaaacgt gctccgcccc gcgtccctgg tgcaccggaa tgacgtgccg
     5881 gtagccgaaa attttccgga cagtttcttc gaaccggtag aagctcttgg ccccggcgta
     5941 cgactcgtcc ccctgcatca ggccggccca ctgggcatcc gacatcgccg cggtgccgga
     6001 gtcagtgagg agatcaatgt aaacgtcttc ggctttgaga ttgaagaggt tgaaatacgc
     6061 ttccctaagc cgcgcttcgc gggtttctct tgaaatcagc cgaattggtt caacaacttt
     6121 tattttgtac ggttctatca gcatctatca ctctccgcta tttcagacgg caaccgcttc
     6181 tcgcactctc gctggcttca ccgcctgcaa cttgaatatc ggcgcaattt gcccgactac
     6241 tcttccgcgg tagttcttaa tgacgggacg ggcaaactcg gccagcttcg gcaccgacat
     6301 atccagcggt acacccagct gactcagcac ctcgatgacg accggagcaa cggctctttg
     6361 actcccgtcg gcaattttga ccgccaggcc ccagccccgg tcaacgaggg cagcgcattc
     6421 cagcgcttcc gccccggctt tgctcagcac ctgcccacgt ccggcttgca tcaaagcgta
     6481 gtcgaagcgc ccgacgccgg aaaccatcaa ggggtgttcg agcatggcct gacggagcgt
     6541 ctggtagacc cggctcgagg cgggagtgct cccgtgggcg gtgacgaaat tagcataccc
     6601 ccgggccata tgccggagag gtaaagcaaa attcggtgcg ctgcagccat caatcgcgac
     6661 gcctatttct accgccggat accggcatgc ctcggctacc gcctgcaaga ttgcttgctg
     6721 cgcgcgactg ttaggatcaa ggtagttttc tttcggttcg tcgtagattt tggacagagc
     6781 cagcatgctg gtgtgctttc cggagcagtt gtggtgcaat tggctgaagc gcgctcccgg
     6841 ctcgggagtc aattcgttag cggtgaaaaa agatggggga tgaatcccgc actgcaagtc
     6901 tccttcgatg aaaccgacct tcatcagcaa ctgttcaacg acttggatgt gctccggctg
     6961 gccggaatgc gacccgcaga taatagccaa ttcctgggaa gtgaggccga agcgctcaat
     7021 gccgccct
//
LOCUS       DBNGZD010000422         3583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_554091, whole genome shotgun sequence.
ACCESSION   DBNGZD010000422 DBNGZD010000000
VERSION     DBNGZD010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_554091"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="AC16GM_10960"
     CDS             <1..481
                     /locus_tag="AC16GM_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2924227.1"
                     /translation="YADAARMYEKALKLDSSDYRVWGSLASTYYWMGARTKAHAKYQR
                     AAQMAEEERKVNPQDATVLSHLADFYSMMGERDKALPLLEQSLALAPPGDVEVMVRAG
                     ETYEQLGQRDKALEWIGKVLRKGYPLAKLERDPGLKELRNDVRFHRLMKELTNEAQN"
     gene            513..821
                     /locus_tag="AC16GM_10965"
     CDS             513..821
                     /locus_tag="AC16GM_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR2924228.1"
                     /translation="MRKPQVHEVKLTRKKGSWKADPAVVRAKAGDSIHWKGTGSDMTL
                     FFPKKKLFWHHNFAIARGRGLVLKVGAGKKGKYPYAAFCHSANCFAEGNSTPIVIIGP
                     "
     gene            1052..2515
                     /gene="lysS"
                     /locus_tag="AC16GM_10970"
     CDS             1052..2515
                     /gene="lysS"
                     /locus_tag="AC16GM_10970"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR2924229.1"
                     /translation="MEIAPELLQHRREKLDRLHQQKINPYPYRFERTHLSSDVVNNFS
                     ELETSGQTVKIAGRLIALRLHGKSLFGHLLDGAGKIQVYVKQDDVGEEQFKLFELFDL
                     GDFLGVSGPVFKTRTGEMTVKAKAFELLAKSLRPLPEKWHGLQDKELRYRQRYLDLVA
                     GPEVKAVFVSRTRILQAIRAFLDSQGFLEVETPILQPLYGGALARPFKTHHHTLDLDF
                     YLRIADELYLKRLIIGGFEKVYEFCKDFRNEGMDKFHNPEFTMVELYWAYADYRGILA
                     LHQELMLFVARQVFGTTQFAYQGQTIELGGQWEEVPYFDGIKKFTGYDLYQKDEKTVR
                     QAAEKLGIDAAKLTTRGKLLDEIFSAKVEPNLIQPTFVTDFPLEISPLAKKHRDKPEL
                     VERYELFIAGLELGNAFSELNDPLDQRRRFEELARLRLLGDEEAAIIDEDFLAALEHG
                     MPPTGGLGFGVDRLVMFFTDQHSIRDVIFFPQMRPEK"
     gene            2516..>3583
                     /locus_tag="AC16GM_10975"
     CDS             2516..>3583
                     /locus_tag="AC16GM_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2924230.1"
                     /translation="MGYEFFIARRYLRAKRHRHFVSAITLISVLGVVVGTAALIVVLS
                     MMNGFEKEIRTRIVGITGHVTIFDQHNEGVAEYQDLGKRVQALPQVVATAPFVYAKAA
                     IASENENDGILVRGVDAEAEKKVTTIGDEMVAGTFDLAEQPGRLSRIILGRGLAERLG
                     VRTGDTVALFSLRGQSLSAGLTQPKVAKFSVGGLFETGMYEYDASLAYINLTRAQSLF
                     EMDGKVTGLQVKTDDLFGAQKVARALENVLGTAYYAQDWMAMHKNLFSWMALEKYAMF
                     VALSLIIAVAAFNIVSSLIMVVLEKRREIGVLKAMGAKREGIKKIFVLMGLITSSVGL
                     VAGFGLGLLLCWLQQTFKIISL"
BASE COUNT          821 a          955 c         1029 g          778 t
ORIGIN      
        1 gtatgccgat gcggcccgta tgtatgagaa ggcgctgaaa ctggatagta gcgactaccg
       61 tgtttggggt tctctggctt cgacttacta ttggatgggc gcgcgtacga aagcgcacgc
      121 taaatatcaa cgcgcggctc agatggctga agaggagcgg aaggtgaatc cccaggatgc
      181 aacggttctt tcgcatctgg ccgattttta ttcaatgatg ggagagaggg acaaggctct
      241 gcccctgttg gaacaatcat tggccctggc gccgccgggt gacgtggaag taatggtaag
      301 agccggggaa acctatgaac agttaggtca gcgtgataag gcgctcgagt ggatcgggaa
      361 ggtcctcagg aaaggttacc cgctggcaaa gctggagcgc gatccggggc taaaggaatt
      421 gcggaacgat gtgcgctttc atcgtctcat gaaagaactt actaacgaag cacagaacta
      481 agcgtagcaa atacgtaaga agggagggtt acatgaggaa accacaagtc catgaagtga
      541 agctcactcg taagaagggc tcctggaaag ccgatccggc tgtggtgcgg gcaaaagcgg
      601 gggattcaat tcactggaaa ggtacaggtt cggatatgac cctgttcttt cccaagaaaa
      661 aactcttctg gcatcacaat ttcgcaatcg caaggggaag agggttagtg ttgaaggtgg
      721 gcgcgggcaa aaaaggcaag tatccctacg ccgccttctg tcacagcgcc aactgttttg
      781 ctgagggaaa ttccactcca atagttatca tcggcccata gaaattccct cgagcgtaac
      841 caggttctcg gcagaacgct agaacatttg ttagcgtgaa ctgaagtaac ctacggatgg
      901 gcgaagccgt agccacgatg gtcctgcgtt agatcacatt cctgattttc cgtcggttgg
      961 atcaaaaaac tcattcgcca ttcgtagtaa ctgacttccc cgagccagct ggctttgcga
     1021 aaacgttccc tcgctgtaaa ttcacctgtc aatggaaatc gctcccgaac tgctccagca
     1081 ccggcgtgag aaattagacc ggttgcacca gcagaaaatc aatccctatc cctaccgttt
     1141 tgaacgcacg cacctgtcca gtgacgtcgt caacaatttt tctgaactgg aaacgtcggg
     1201 acagacggta aaaattgccg ggcgactcat cgccctgcgc ctgcacggca aatcgctctt
     1261 cggccacctc ttggatggcg ccgggaaaat ccaggtctac gtcaaacagg atgatgtggg
     1321 agaagagcaa ttcaagctgt ttgagttatt tgacctgggc gattttctgg gcgtcagcgg
     1381 gccggtcttc aagacgcgaa ccggcgaaat gacggtaaaa gccaaagcgt tcgaacttct
     1441 ggccaaatcg ctccgtcccc tgccggagaa gtggcatggc ctgcaggaca aagaactccg
     1501 ctaccgtcag cgctacttag acctggtggc cggccccgag gtgaaagcgg ttttcgtctc
     1561 ccggacgcgc atcctccagg ccatccgagc ctttctggac agccaggggt ttctggaagt
     1621 tgaaacgccg atcctgcagc ctttgtacgg cggggcgctg gcccggccgt ttaagaccca
     1681 ccaccatact ctggatcttg acttctacct gcggattgcc gacgaactct acctgaagcg
     1741 cttgatcatt ggtgggtttg agaaagttta tgaattctgc aaggatttcc gtaacgaggg
     1801 tatggacaag ttccacaacc cggagttcac catggtggaa ctctactggg cctacgccga
     1861 ttaccggggc attctggcgc tccaccaaga gttgatgctc tttgtcgccc ggcaggtttt
     1921 cggcaccacg cagtttgctt accagggcca gacgattgag ctgggcggcc agtgggagga
     1981 agttccctat tttgacggga tcaaaaaatt caccggatac gatttgtacc agaaagacga
     2041 aaagacggtc cgccaggccg cggaaaaact gggtatcgac gcggccaaac tcaccacccg
     2101 cgggaaactc ttagacgaaa ttttctccgc gaaggtggag ccgaatttga tccagccgac
     2161 tttcgtcacc gattttcccc tggaaatctc gcccttggcc aagaagcacc gcgacaaacc
     2221 agagttggta gagcgctacg aacttttcat cgccggactg gaattgggga acgccttttc
     2281 ggaattgaat gacccgcttg accagcgccg gcgttttgag gaattggccc ggctgcgcct
     2341 tttgggtgac gaagaggccg caatcatcga cgaagacttt ttggccgcct tggagcacgg
     2401 gatgcccccc accggcgggt tgggcttcgg cgtcgaccgg ctggtgatgt tcttcaccga
     2461 ccagcactcg atccgggacg tgattttctt tccccagatg cggccggaaa agtaaatggg
     2521 ttacgaattt ttcatcgccc ggcgctacct gcgggccaaa agacaccggc acttcgtctc
     2581 ggccatcacc ttgatttcgg tcctgggtgt cgtggtcggt accgcggctt taatcgttgt
     2641 gctctcgatg atgaacggtt ttgagaaaga aatccgcacc cggatcgtcg gcattaccgg
     2701 ccacgtcacg atcttcgacc agcacaacga aggcgttgcc gaatatcaag atttagggaa
     2761 acgggtccag gcgctgccgc aggtagtggc tacggctccc tttgtctatg ccaaagcggc
     2821 catcgcgtca gaaaacgaga acgacggtat tctggtccgc ggtgttgatg ccgaagcgga
     2881 aaaaaaagtg acgacgatcg gcgacgagat ggtcgccggc acttttgact tggccgaaca
     2941 accgggccgg ctctcccgca tcatcttggg ccgcggcctg gccgaacgtc tgggggtgcg
     3001 caccggcgac acggtggcac ttttcagcct gcgcggtcag agtctttcgg cagggttgac
     3061 ccagccgaag gtggcgaagt tcagcgtggg cggcctgttt gaaaccggga tgtacgagta
     3121 cgatgcctcc ctcgcctaca tcaacctgac ccgggcgcaa tcgctcttcg aaatggatgg
     3181 caaggtgacc ggactgcagg tcaaaaccga cgacctcttc ggcgcccaaa aagtggcccg
     3241 cgcgcttgag aacgtgctcg gcaccgctta ctatgcccag gactggatgg cgatgcacaa
     3301 gaacctcttt tcctggatgg ccttggagaa gtacgccatg ttcgtcgcct tgagtctcat
     3361 tatcgccgtc gccgccttca acatcgtgtc gagtctgatc atggttgtcc tggaaaagcg
     3421 ccgggaaatc ggcgtcttaa aagccatggg cgccaaacgt gaaggaatca aaaagatttt
     3481 cgtcttgatg ggattgatta cctcgagcgt cggcctggtc gccgggttcg gcctggggct
     3541 tctcctctgc tggttgcagc agacgttcaa aatcatttcc ttg
//
LOCUS       DBNGZD010000423         6164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1941447, whole genome shotgun sequence.
ACCESSION   DBNGZD010000423 DBNGZD010000000
VERSION     DBNGZD010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1941447"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="AC16GM_10980"
     CDS             complement(<1..170)
                     /locus_tag="AC16GM_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR2924231.1"
                     /translation="MWTSDWKRTHTCGQLRQADGGKKVTLNGWVYRWRNHGGLVFIDL
                     RDRYGVTQVVFNP"
     gene            448..1128
                     /locus_tag="AC16GM_10985"
     CDS             448..1128
                     /locus_tag="AC16GM_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR2924232.1"
                     /translation="MLDWLAAELTILKNIILFLLPAVPATIEVTLIAFALALVLGQLI
                     GLLRISRRKVFGITSKIYVDTIRGIPLLVQIFFIYFGLGKVLNLDRFLAGVVAIGVCY
                     SGYLAETFRAGIQAIHKGQYEAALSLGLTRWQMMRHVILPQSLRIVIPPVANDFIACL
                     KDSSLVSVIGLRELTRAGRDYYSQFFVDFQTWLIVGLLYLVMTLTLSRFVAFLEKKFA
                     VHGFGVVH"
     gene            1268..1999
                     /locus_tag="AC16GM_10990"
     CDS             1268..1999
                     /locus_tag="AC16GM_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR2924233.1"
                     /translation="MSIIALKNVSKRFNQVVALDGVSLSVEPGEVIVLVGPSGSGKST
                     LLRCMNALEIPDSGEVIVDGIMLDHKKAHIYAIRRETGMVFQQFNLFPHLSVLENIYL
                     AQRVVRKRTKAEAGKIAETLLEKVGLLEKKAAYPGQLSGGQQQRVAIARALATNPKIM
                     LFDEPTSALDPEMIGEVLGVMKKLAREGMTMVVVTHEMGFAREVAHRIAFLDEGKLVE
                     IGPVEKVLRAPENPRTQAFLSKVLH"
     gene            2113..5328
                     /locus_tag="AC16GM_10995"
     CDS             2113..5328
                     /locus_tag="AC16GM_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR2924234.1"
                     /translation="MLKRALFLFLGFGIVRVSASQADSYWQQEVAYTIDCQLFPKGDS
                     LTGTETLVYTNHSPDTLTYVYFHLYLNAFQPGSYMDLRSREAGNFSLRDLPKRDWGEE
                     RIDSLAIEGERWTKSELDNTILKVDLPKPLLSGEKIAFFFKFVDKIPRNGSRMGKQGR
                     HYDIGQWFPRLAAYDKRGWHDDQYLGSEFYADYGTFDVSITVPKEYVLAHTGKLTNEE
                     ELFPGGLPKAEGDSVVIDILKLYKEQHPSEKQIAFDSTNPDTSASPSIRQRPTRGGKR
                     AEPRHPEMQNPNGDSSPLDTAGKKNEPKTWTWKCHADTVFDFAFAADPDFIWDRAQWE
                     GIVMNALYQKDKQEFWQKWGAIYTREIIKFYSQTFGMWPYPQFSSVEGSVGGGIEYPQ
                     MTIITRRSGSKYSHGLFAILAHEIGHNWFYGLLGNNETRQAFLDEGFTSFATCLAMEH
                     YYGRYDNNYTWEKWYQKKFYPNGDERKDTFFSYLRFAKTGTEDVIDTHSDRFLPGSYT
                     NAVYPKTASILFMLQYVLGDSVFAKAMHDYYDRWHYKHPHKEDFYASIEKSSGCHLEW
                     FFQEWFERVYTCDYSLEGVKSELKDPNRPAPYDAEVKIKRRGRAIMPLDVQLTLADGQ
                     KVSRDIPFDLWMKGPTLQTITIPVSSPVKRAVLNPDLRVQDVNRLNDYHPRAPVTFQF
                     QPPQFLFPGYSPDPPLDAYQIRYQPKLWYNQEDVARLGFGGFGSYLGVDHRLNTDLWV
                     GVRHAQVDFNVNYTSPFYLFDNRTTWSAGGSFLEGRQGASLSLSRTYGRSGFFGFGGF
                     TAAPPRRVSLSWNYSRLDNPDYLSRTQSWSSGRVSTWKFSWNAVFNRWKFDSRIALEL
                     TSAALRTQHDFGKAALEIESDWHTPDKFPLWSRLYFGYAEGDVPLQDLFYVASASPDE
                     QFGSRWYSSRGSLPEGLKRTGNLYLPGGGNLRGFLKKNAGYKRIASANVRLGFENPLT
                     KLLTKIHQGSEILWRDLTSIETHAFFDFGQGWNSGNIPDLGDFFSDLGLSLSYRFPYQ
                     SIRRLVGDNYIRVYFPLWISDPLPGESRWKFRWVLAYAAKF"
     gene            5434..>6164
                     /locus_tag="AC16GM_11000"
     CDS             5434..>6164
                     /locus_tag="AC16GM_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024685.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HIR2924235.1"
                     /translation="MDDFSIQVRNDQNFPYMERELLEKMLKKEYDLEFLLQELEEISS
                     KEYLPALSGYTQPAESLSLKLKGEIFKHYSPIIAGDAAEYLKWVLEHSHFTFQLLNST
                     KDTEILPIFTTNYDPAIEEACKFGNFWQRNINFCNGIIQDFNSQEFVWDRRGFEFNPD
                     PRKKQICLFKLHGSVDWYRINGKIVRVPFSEHHGDDDIIRNVIIYPAMKKVAIAEPFL
                     TAYNYLHHCLLNTNVAVFIGYSFRD"
BASE COUNT         1412 a         1770 c         1580 g         1402 t
ORIGIN      
        1 gggttgaaga caacctgggt aacgccgtag cggtcgcgca ggtcaatgaa gaccaggccg
       61 ccgtggttgc gccagcggta tacccagccg ttcagggtca cttttttgcc gccgtccgcc
      121 tgccgcagct ggccgcaggt gtgcgtgcgc ttccagtcag aagtccagat gaccggctgg
      181 ggagaggtag gcgtcagggg ttgggtttga ctacttacgg aatctgccac aggtttcagt
      241 ttaagaataa tgcccgacga gctagaagtc aaacgctcta caaaaaccgt ttgttgccgc
      301 aagagccata gtaatcttat cggtcaagtg agaagaagtt atataaaacc gtagccgcgg
      361 cgccctcgcc acgtaaatcc actcttcaga ataagtgagg aggcttgaca ctacccaact
      421 tttcctttct ttgaccagtc agcacctatg ctcgattggc ttgccgccga actgactatt
      481 ctgaaaaaca tcatcctttt ccttttaccc gccgtcccgg ccacaattga agtcacgctg
      541 attgcttttg ccctggcctt ggttctggga caactcatcg gccttttgcg catctcccgg
      601 cgcaaagttt tcgggataac gtcaaaaatc tacgtcgata ccatccgcgg catcccactt
      661 ttggtgcaga ttttcttcat ctacttcggc ctgggcaaag tgctgaattt ggaccgcttt
      721 ctggctgggg tagtcgccat cggcgtctgt tactccggct acctggccga aaccttccgc
      781 gccggaatcc aggccatcca caaaggccag tacgaagcgg ccttgtcgtt gggcctcacc
      841 cgctggcaga tgatgcgcca tgtcatcctg ccccagtcgc tgcgcatcgt tattccgccg
      901 gtggccaacg acttcatcgc ctgcctcaag gatagttcac tcgtgtcggt gatcggcctg
      961 cgcgaactga ctcgcgccgg gcgggattac tattcccagt tcttcgtcga tttccagacc
     1021 tggctcatcg tcgggctgtt atatttagtg atgacactca ccctgtcacg atttgtggcg
     1081 tttctggaga agaaatttgc cgtgcacgga tttggagtgg tgcattaaat aagaattcac
     1141 agcagatgaa gcagatgcac gatactccca ttcgtagcgc aggttggttt tggaaacttg
     1201 cgccgcctct gagtcaatca agcgcgggat acgaaaacca tcccgcgcta cggtaaaagt
     1261 cggcataatg tccatcatcg cactcaaaaa cgtctctaag cgcttcaacc aagtggtggc
     1321 cttggatggc gtatctcttt cagtcgagcc cggcgaggtg atcgttttgg tcggcccgtc
     1381 cggatcgggg aagagcacgc ttttgcgctg catgaacgcg ctcgaaatcc cggactccgg
     1441 cgaggtaatc gttgatggaa tcatgctgga tcacaagaaa gcccacatct acgccatccg
     1501 gcgcgaaacc ggcatggtct tccagcagtt caaccttttc ccgcacttga gcgttttgga
     1561 gaatatctat ctggcccaac gggtggtgcg caagcgcacg aaagctgaag ccgggaaaat
     1621 tgcggaaact ctcttggaaa aagtggggtt gctggagaag aaggccgctt accccgggca
     1681 gctctccggc ggccagcagc agcgggtggc cattgcgcgc gccctggcta ccaatcccaa
     1741 gataatgctc tttgacgagc cgacctcggc cttggacccg gaaatgatcg gcgaggtttt
     1801 gggtgtgatg aaaaagttgg cccgggaagg aatgacgatg gtcgtggtga cccacgaaat
     1861 gggctttgcg cgggaagtgg cccaccggat cgcctttctg gatgaaggca agttggtcga
     1921 gatcgggccg gtcgagaaag tgctgcgggc tcccgaaaat ccgcgtacgc aggctttttt
     1981 gagcaaggtg ctgcattgaa aactacaaag cattcacagc cgatggagca aatgaaacag
     2041 acactgacca ggacatccgc ttcatcggct gcgattcccg ttttcccatt ccaacccaac
     2101 tggaggtcaa tgatgctcaa acgcgctctg tttcttttcc ttggattcgg catagtgagg
     2161 gtcagcgcaa gtcaggctga ctcctactgg cagcaagaag tggcttacac catcgactgc
     2221 cagttgttcc cgaaaggcga ctccctcacc ggcaccgaaa cgctcgtcta cacgaaccac
     2281 tcgcccgaca cgctcactta cgtttacttt cacctctacc tgaacgcctt ccagccgggg
     2341 agctatatgg acctgcgcag ccgggaagcc ggcaatttct ccctgcgcga cctccccaag
     2401 cgcgactggg gcgaagagcg gattgattct ctggcaattg agggtgaacg gtggacgaaa
     2461 agcgaattag acaacacgat tttgaaagtc gatctgccca aacctctttt gtccggtgaa
     2521 aaaatcgctt tctttttcaa gttcgtcgac aaaatccccc gcaacggctc ccggatgggc
     2581 aagcaggggc ggcactacga catcggccag tggttcccac gcctggcggc gtacgacaaa
     2641 cgcggctggc atgacgacca gtacttaggg agcgagttct acgccgacta cggcaccttc
     2701 gacgtgtcga taacggtacc gaaagagtat gtcctcgccc acaccggaaa actcacaaac
     2761 gaagaggaac tcttccccgg cggcctgccg aaggccgaag gtgattcggt tgtcattgat
     2821 attctgaaac tctataagga gcagcacccg tccgagaagc aaatcgcctt cgattccacc
     2881 aaccccgaca cctcggcctc cccttcgatc cgccaaaggc cgactcgggg cggtaagcgt
     2941 gccgaaccgc gacaccccga aatgcagaat ccaaacggtg attcgtcccc acttgatact
     3001 gccgggaaaa agaacgagcc caaaacctgg acctggaagt gccacgccga caccgtcttc
     3061 gatttcgcct tcgccgccga ccccgatttc atctgggacc gcgctcagtg ggaaggcatt
     3121 gtgatgaacg ccctctacca gaaggataag caggaattct ggcaaaaatg gggcgcgatt
     3181 tacacccggg agatcatcaa gttctacagc cagaccttcg ggatgtggcc ctacccgcag
     3241 ttttcgtcgg tggaaggttc cgtcggcggc ggcatcgagt acccacagat gacgattatc
     3301 acccggcgca gcgggtcgaa atacagtcac gggctctttg cgattctggc ccacgaaatc
     3361 ggccacaact ggttctacgg cctgctcggc aacaacgaaa cccgccaggc gtttttagac
     3421 gagggcttca cctcctttgc tacttgcctg gccatggagc actactacgg ccgctacgac
     3481 aacaactaca cctgggagaa gtggtaccag aaaaaattct atcccaatgg cgacgagcgc
     3541 aaagacacct tcttttctta cctccgcttc gccaagacgg gaaccgagga tgtcatcgac
     3601 acccactccg accgcttcct gcccggctcg tacaccaacg ccgtttaccc caagaccgct
     3661 tccatcctct tcatgctcca gtatgtcttg ggagattcgg tttttgccaa agcgatgcac
     3721 gactactacg accgctggca ttataaacat ccccacaaag aggattttta cgcctccatc
     3781 gaaaaatcga gcggttgtca cttagaatgg ttctttcagg agtggttcga gcgggtttac
     3841 acctgcgact acagcctcga aggagtcaaa tccgaactga aagatccgaa tcgtcctgct
     3901 ccttatgacg cggaggtgaa aatcaaacgc cggggccggg ccattatgcc gttggacgtt
     3961 cagttgacgc tggccgacgg ccagaaggtc agccgggaca ttccgttcga tctctggatg
     4021 aagggaccga cgttacaaac gattaccatc cccgtctctt ctccggtcaa gcgggcggtc
     4081 ttaaaccccg acctgcgggt gcaggatgtc aaccgcttga acgactacca cccgcgggct
     4141 ccggtcacgt tccaatttca gccgccgcaa tttctctttc cgggctactc gcccgacccg
     4201 cctctggatg cctaccagat ccgttaccag ccgaaactct ggtacaacca ggaggacgtg
     4261 gctcgtttag ggtttggcgg cttcggttca tacttaggtg tcgatcaccg gttgaatacc
     4321 gacctctggg tcggcgtccg ccatgcgcag gtcgatttca acgtcaacta cacttcgccc
     4381 ttctacctgt tcgacaaccg gacaacctgg tcagccggcg gctcatttct cgaaggccgg
     4441 cagggggcct cactttctct gtcgcgcacc tacggacgga gcggcttttt cggcttcggc
     4501 ggctttaccg ccgcaccccc gcgccgcgtc agtttgtcgt ggaattactc gcggctggac
     4561 aatcccgact acctctcccg cacccagtcc tggtcgtccg gacgggtctc gacctggaag
     4621 ttttcctgga atgccgtgtt caacaggtgg aagttcgaca gccgcattgc gctggagctg
     4681 acttccgcgg ccctccgcac gcaacacgac tttggcaagg ccgccctgga aatagaaagt
     4741 gactggcata cccccgacaa attcccgctc tggtcccggc tctatttcgg gtatgccgaa
     4801 ggcgacgtgc ccctgcagga cctcttctac gtggcctccg ccagtcccga tgagcagttc
     4861 ggcagccggt ggtactccag ccgcggctcc ttgcccgaag gcttgaagcg aaccggaaac
     4921 ctctaccttc ccggcggggg caacctgcgc ggctttctca aaaagaatgc cgggtacaaa
     4981 cgcatcgcca gcgccaatgt ccggctcggt ttcgagaatc cgctgacgaa actgttgacc
     5041 aaaatccacc aaggcagcga aatcctctgg cgcgacctca cctccataga aacccacgcc
     5101 ttcttcgact ttggccaggg gtggaattcc ggcaacatcc ctgatctcgg tgactttttc
     5161 tctgatctcg gactttcgct ttcctaccgc ttcccgtacc agagtatccg gcggctggtg
     5221 ggcgacaact acatccgcgt ttatttcccg ctctggatct ccgatcctct gccgggcgag
     5281 agcagatgga agttccgctg ggtgctggcg tatgcggcga agttctaaca atgctcgttg
     5341 accatgaata ggctcataac ggatgcaccc ctaattttct ttttgggtgc gggagctaca
     5401 aaatgcttag gaaaacgctt gatgagagat ttcgtggacg acttttcgat tcaagtgcga
     5461 aacgatcaaa atttccccta catggaacgc gaactgttgg aaaaaatgct taagaaagaa
     5521 tatgatctag aattcctctt acaagaactg gaagagattt cctccaaaga gtatttaccc
     5581 gctctttctg gttatactca acctgctgaa tctttgtctc ttaagctcaa aggggaaatt
     5641 tttaagcatt acagcccgat tattgctggt gatgcagcgg aatacctgaa atgggtacta
     5701 gaacattcgc acttcacttt ccaactatta aactccacga aagatacaga aattttgcct
     5761 attttcacta cgaactacga ccccgctatt gaagaagctt gcaagtttgg aaatttctgg
     5821 caacgaaata taaatttttg caatggcatc attcaggact tcaattctca agaatttgtc
     5881 tgggatagaa gaggatttga attcaaccca gatcctcgga aaaaacagat ctgtctcttt
     5941 aaattacacg gttcagtcga ttggtacaga ataaatggaa aaatcgttcg ggtaccattt
     6001 tcggaacatc atggagacga tgatattatt agaaacgtca ttatctatcc tgccatgaaa
     6061 aaggtggcca tagccgagcc gttccttact gcatacaact atctccatca ttgtctgcta
     6121 aatacaaatg tggccgtttt cataggttac tcctttcggg acta
//
LOCUS       DBNGZD010000424         3832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_762117, whole genome shotgun sequence.
ACCESSION   DBNGZD010000424 DBNGZD010000000
VERSION     DBNGZD010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_762117"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="AC16GM_11005"
     CDS             <1..314
                     /locus_tag="AC16GM_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR2924236.1"
                     /translation="LRKSFLAALGVVSLTRKKAEEVVDQLIKEGELQRSQRTQAIEEL
                     LEKAEARIKETGKRVEQKTRETVTRMRGKLDLNFDNLQSRISRLEEKVDELIEELRKR
                     V"
     gene            complement(489..1211)
                     /locus_tag="AC16GM_11010"
     CDS             complement(489..1211)
                     /locus_tag="AC16GM_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2924237.1"
                     /translation="MNTNNILLEVKNVNVYYGGIHALKDVSFQIHQGEIVTLIGSNGA
                     GKTTTLNTISGLLRPKAGEIRFAGEDLTRVAPHETVKRGISQAPEGRKIFANMTVKEN
                     LELGAYLRRDKAGIAADFERVFTLFPRLKERLNQNAGTLSGGEQQMLAIGRALMSHPK
                     LLLLDEPSLGLAPLLVKAIFQVIAEINQQGTTILLVEQNANLALRCASRGYVLETGRV
                     VLADTTPALLQNEQVKRAYLGG"
     gene            complement(1208..1969)
                     /locus_tag="AC16GM_11015"
     CDS             complement(1208..1969)
                     /locus_tag="AC16GM_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2924238.1"
                     /translation="MLTLDHVSLSFGGLKAVDRFCLSMNPGELVGLIGPNGAGKTTVF
                     NLITGVYEPDEGAIDFCNCRLNGLKPYQITALSIARTFQNIRLFGNLTVRENVQIACH
                     LHYSSGLPAVLWRSPSSRMAEFEQREKTKRLLEIFELAGVAEEKAKNLPYGQQRRVEV
                     ARALATDPKLLLLDEPAAGMNPQETQKLMQLIRWLKDEFKLTILLIEHDMHVVMGICE
                     RILVLDYGETIAQGPPDAIRKNPRVIEAYLGEEPV"
     gene            complement(2043..3080)
                     /locus_tag="AC16GM_11020"
     CDS             complement(2043..3080)
                     /locus_tag="AC16GM_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2924239.1"
                     /translation="MDTTRTGTNLRYWTKQVTFVFGGLALFLLLNNLLAPQLDPYILR
                     LIILSGINIILAVSLNLINGFTGQFSIGHAGFMAIGGYCAAALTVYGGPSILGALSGL
                     GLPPVAARSILFLVSLLVGGLFAAIAGVLVGIPSLRLRGDYLAIVTLGMGEIIRVIIL
                     NTEAIGGARGFACPLYTDLFWVLLWTVLTIVIIYNLLHSSHGRAFLSVREDEVAAEAM
                     GINTTYYKVTAFVIGAFFAGIAGGLFGHYLQLLHTNSFTFMKSFEIVIMVILGGMGSL
                     SGSIIAAVIITLLPEVLRPVKEYRLVLYSALLIVLMLTRPQGLFGTEELSWRRISGWF
                     KRKPKIQPGVA"
     gene            complement(3086..>3832)
                     /locus_tag="AC16GM_11025"
     CDS             complement(3086..>3832)
                     /locus_tag="AC16GM_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2924240.1"
                     /translation="FSHWLGISDHPSLGGALAILFLAMALCALLGVIIERGAYRPLRK
                     QPRLTVLITAIGVSLLLENGGQLVFGANPKFFPSLIADRELVNTGGVVIRLLPVVVLA
                     VSLVLMLFLRFIVVKTKIGKAMRAVSYNREAASLMGIPTDNVITFTFILGSALAGAGG
                     VLVASTYPKIDPLMGLLPGLKAFVAAVLGGIGNIPGAVLGGMILGVSETMVAGYISST
                     LRDAIAFGLLVFILLVKPTGLLGSPVSEKV"
BASE COUNT          945 a         1010 c         1166 g          711 t
ORIGIN      
        1 tgctgagaaa gtcgttcctg gcggctttag gcgtagtttc cctgacgcgg aaaaaggctg
       61 aggaggtcgt cgaccagctc atcaaagagg gagagttgca gcgcagccaa aggacacagg
      121 caattgaaga acttctcgaa aaggcggaag cgcggatcaa ggaaaccggc aagcgggtag
      181 aacagaaaac gagggagacc gttacccgga tgcgcgggaa actggatctg aattttgata
      241 atctccaatc acgcatcagt cggttagaag agaaagtcga cgaattaatc gaggagttgc
      301 ggaagagagt ctaagaattt caccacggag acacagagga cacagagaaa aacatcgcct
      361 ggtcaatgat agacccgtcg gtgaggagag cggcgggtct ccgcacaatt agggcaacag
      421 cgcggaacta aagtttcggt acgagtcgtg ccgacccgcc tacgcgccaa aacctcggga
      481 agcaagggtc aaccgcccaa ataggcgcgt ttgacctgtt cgttttgcag cagggccggg
      541 gtcgtatcgg ccaggaccac ccgtccggtc tccagcacgt aaccacggct ggcacagcgc
      601 agagccagat tggcgttctg ctcgaccagc agaatagtcg tgccctgctg gttgatttcg
      661 gcgatgacct gaaagatggc cttgaccaaa agcggcgcca gacccaggga gggttcgtcc
      721 agtagcaaga gtttcgggtg ggacatcagg gcgcggccga tggcgagcat ttgctgctcc
      781 ccgccggaaa gcgtgccggc attttgattc agccgctcct tcaatcgagg aaaaagggtg
      841 aagacccgtt cgaaatcggc ggcgatgccg gccttgtccc ggcgcaggta ggcgcccagt
      901 tcgagatttt ccttgaccgt catattggcg aaaattttgc ggccttccgg cgcctgagag
      961 ataccacgct tcaccgtctc gtggggcgca acgcgggtga ggtcctcgcc cgcgaagcgg
     1021 atttcgcccg ctttggggcg caaaagaccg gagatagtat tgagcgtggt ggttttgccc
     1081 gcgccgttgg agccgattaa ggtaacaatt tctccctgat gaatctgaaa ggagacgtct
     1141 tttaaggcat gaatcccgcc ataatagacg ttcacattct ttacttccaa taaaatgtta
     1201 ttagtgttca caccggctcc tcgcccaaat aggcttcgat cactctgggg tttttccgga
     1261 tagcgtccgg tgggccctgg gcaatggttt caccgtagtc gagcaccagg atgcgctcgc
     1321 agatgcccat caccacgtgc atgtcatgct cgatcagaag aatggtgagt ttgaactcgt
     1381 ctttcagcca gcggatcaac tgcatcagtt tctgcgtttc ctgcgggttc atgccggcgg
     1441 cgggctcatc caaaagcaaa agtttcggat cggtggccag ggcccgggca acttccacgc
     1501 gccgctgctg gccgtagggg aggtttttgg ctttttcttc ggctacgccg gctaattcaa
     1561 aaatttccaa aagtcttttg gttttttccc gttgctcaaa ttcggccatt ctacttgagg
     1621 gagaccgcca gaggacagcg ggcagtcccg acgagtaatg gaggtggcag gcgatctgca
     1681 cgttttcgcg cacggtgagg ttcccgaaca ggcggatgtt ctgaaaggtg cgggcgatgg
     1741 agagcgcagt aatttgatac ggcttcagcc cgttcaatct gcaattgcag aagtcaatcg
     1801 ctccctcatc cggctcgtag acgccggtga tcaggttgaa caccgtggtt tttcccgccc
     1861 cgttggggcc gatcaaaccc accagttcgc cggggttcat actcaggcaa aagcggtcta
     1921 ccgccttgag gccgccgaag gagagagaaa cgtggtcaag ggttagcaca ggtaaaagat
     1981 agtgtcgaag tgtcggagtg tcgaggtgtc ggagtaaaaa caagatgcac gcccagaagg
     2041 ggctaggcta cgccgggttg aattttgggt ttgcgcttga accaaccaga tatgcgtcgc
     2101 caggagagtt cttcggtgcc gaagagaccc tgggggcggg tgagcatcaa aacaatcaac
     2161 aaggctgagt agagcaccag acggtattct ttcaccgggc gcagcacctc gggcaaaaga
     2221 gtaataataa cggcggcgat gatggagccg gagagcgacc ccatcccgcc caggatcacc
     2281 ataatcacga tttcaaacga tttcatgaaa gtaaaggaat tcgtgtgcaa gagttgcagg
     2341 tagtggccga acagcccgcc ggcaatgccg gcaaaaaagg cgccgatcac aaaggcagta
     2401 accttgtagt aggtcgtgtt tatgcccatg gcttcggcgg ccacttcatc ttcccgcacc
     2461 gacagaaaag cccggccatg ggaggagtgc agcaggttat agataatcac aatcgtgagc
     2521 acggtccaga ggagcaccca gaagagatcg gtataaaggg ggcaggcaaa gccgcgcgcg
     2581 ccgccaatcg cttccgtgtt cagaataatg acccggatga tttcacccat ccccagggtg
     2641 acaatggcca ggtagtcgcc gcgcaaacgc agagacggta tccccaccag aaccccggcg
     2701 atggccgcaa agaggccgcc taccagaaga ctcaccagaa acaaaatcga gcgtgccgcc
     2761 accgggggga gccccagccc ggagagggcg cccaaaattg acgggccgcc gtaaacggtc
     2821 aaagccgccg cacagtaacc gccaatggcc ataaagccgg cgtggccgat ggaaaactgg
     2881 ccggtgaagc cgttaattaa attcaaactg accgccagga taatgttaat gccggagaga
     2941 atgatcagtc gaaggatata gggatcgagt tgcggtgcca ggaggttgtt aaggagcaaa
     3001 aagagggcca ggccgccgaa aacgaaagtg acctgtttgg tccagtagcg caggttggtc
     3061 ccggtacgag tcgtgtccaa cacggctaca ctttctcgct caccggggaa cccaaaagtc
     3121 cggtcggttt caccaagaga atgaatacta aaagaccgaa agcgatggcg tcgcgcaggg
     3181 tcgaggagat gtagccggcg accatagttt ccgacacccc cagaatcatc ccgcccagaa
     3241 cggcgccggg aatgttgccg atgccgccta aaacggcggc gacaaaggcc ttgagtcccg
     3301 gcaaaagacc cattaagggg tcaattttgg ggtaggttga agccaccaag acgccgcccg
     3361 ccccggccag ggcggagccc agaatgaagg taaaggtaat cacgttgtcg gtgggaatgc
     3421 ccatgagact cgcggcttcg cggttgtacg ataccgcgcg catcgctttg ccgattttgg
     3481 ttttgacgac gatgaaacgc aagaagagca tcagtaccaa agagaccgcc agcactacca
     3541 ccggcaggag gcgtattact acgccgccgg tgttcaccag ttcgcggtcg gcaatcaacg
     3601 atggaaagaa ttttggattg gcgccaaaga ccagttggcc gccgttctcc agaaggagcg
     3661 agacccctat ggcggtgatc agaaccgtca gtcgcggctg cttgcgtagc gggcggtagg
     3721 cgccgcgctc tatgatcacg ccgagaaggg cacagagggc catggccaga aagaggatcg
     3781 ccagggcacc ccccagggag gggtggtcgg agatgccgag ccagtgggaa aa
//
LOCUS       DBNGZD010000425         2767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_690632, whole genome shotgun sequence.
ACCESSION   DBNGZD010000425 DBNGZD010000000
VERSION     DBNGZD010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_690632"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..840)
                     /locus_tag="AC16GM_11030"
     CDS             complement(34..840)
                     /locus_tag="AC16GM_11030"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR2924241.1"
                     /translation="MTETIQFLIRYGYIVLFISVFAEQIGLPLPAIPLLLAAGALAGL
                     GRLSFALVVVLAVAGSLVADFIWYEIGRRRGGRVLNFLCRISLEPDSCVRRTENFFAR
                     YGARSLLLAKFIPGFSVVAPPLAGIFGMRLGRFLSFTALGALIWAGTFTGVGYLFSSQ
                     LEQVATYAGQFGASLVAVLVGGLALYLAWKYLQRRHFLAQLRVARITPEELKQKLDAG
                     DEMIIVDLRQSLDFEAEPHTIPGAFHLPAEHLDARHHEIPRDREIVLYCS"
     gene            complement(951..>2767)
                     /locus_tag="AC16GM_11035"
     CDS             complement(951..>2767)
                     /locus_tag="AC16GM_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIR2924242.1"
                     /translation="GARFYRGFWAQLKHGRADMNTLIAIGTSAAYFYSVAVTFSPSGF
                     TSAGHRAEVYYETAAAIIALILLGRYLETQAKGRTSAAIKKLLGLAPKTARVRRDGSE
                     MDLPIDQVFVDDTVIVRPGERIPVDGQVIEGYSAIDESMVTGESLPVEKKIGDRVIGG
                     TINKTGSFVFRATKVGKDTLLAQIIHLVEKAQGSKAPIQKLADRVAAVFVPIVLVIAA
                     ITFAVWFLFGPEPKLTRALLNCVAVLIIACPCAMGLATPTAIMVGTGRGAEMGILIKD
                     AGALEKAQKLDTVVFDKTGTVTSGKPAVTAVFPLNGFAPEQVVRWAASAEKRSEHPLA
                     EAVTRYAEEKKFALSEPTAFTAEPGFGLVATVEAKRILVGNEALLRRERISLDGVSAK
                     QFAANSGASFLWVAVDGRVAGALTVADTLKPNAVSAVVRLKSLGLEPVLASGDREETT
                     VEIAKKLGIVAYYAQILPQEKGDLVRLLQQRGKKVAMVGDGINDAVALAQADVGIAMG
                     TGTDVAIETADITLVKGDLSGVARAIALARKTVKTIRWNLFWAFIYNIIGIPIAAGAL
                     YPFFGWQLNPMLAAAAMAFSSVFVVTNSLRLRKFQEGV"
BASE COUNT          638 a          854 c          745 g          530 t
ORIGIN      
        1 aagcgccacc cgggcgctgg ttgcttcgtt cggtcaggag cagtagagaa caatttcgcg
       61 gtcgcgggga atttcgtggt gccgcgcatc gagatgttcg gcgggcaggt ggaacgcccc
      121 gggaatcgtg tggggttcgg cctcgaagtc taaggactgg cgcaaatcga cgataatcat
      181 ttcatcacca gcatcgagtt tttgctttaa ttcttccggc gtaatgcgcg ccacgcgcag
      241 ctgagccaaa aagtgccgcc tttggaggta cttccaggca agatagaggg ccagcccgcc
      301 gactaacacc gccaccagcg acgccccaaa ctgccccgca taggtagcca cctgctccaa
      361 ctggctgctg aacaaatacc cgacgccggt aaatgttccg gcccagatga gagcgccaag
      421 cgctgtgaaa ctgaggaacc gccccaaccg catgccgaag atcccggcta atggcggcgc
      481 caccacgctg aatcccggaa tgaacttggc caaaaggagc gaccgtgcac cgtaccgcgc
      541 aaaaaagttc tcagtgcggc gcacgcagga gtcgggttca agcgaaatcc ggcagaggaa
      601 gttcagcacc cgccctccgc ggcggcgacc gatttcgtac cagatgaaat cagccaccag
      661 cgacccggcc accgccagaa cgacgaccaa agcgaaactc aaccgaccca gacccgccag
      721 agctccggcg gccaaaagca ggggaatggc cggcaaaggc aacccgatct gctcggcaaa
      781 aaccgaaatg aagagcacga tgtacccgta acgaatgaga aattgaattg tctcggtcac
      841 tcgatctttc ctcccatgcg ttttgttaaa attgtagggg tgaacattta ggttcacccg
      901 acacattttg ggcaaatcta cagattcgcc ccgacactaa attgctgaga ttacacaccc
      961 tcctgaaatt tcctcagccg gagcgaattg gtcaccacga agaccgagga aaacgccatc
     1021 gccgcagcgg caagcatcgg gttcaactgc cagccgaaga aagggtagag cgcccccgcg
     1081 gcaatcggaa tcccgatgat gttgtagatg aaagcccaga acaagttcca gcggattgtc
     1141 ttcacagttt tacgtgcaag ggcaatcgcc cgcgccacgc cggagagatc acctttgacc
     1201 agagtaatgt cggcggtttc gatggccacg tcggtaccgg tgcccatggc aatgcccacg
     1261 tcggcttgag ccagggccac tgcgtcgttg atcccatcgc ccaccatggc cacttttttg
     1321 ccacgctgtt ggagaaggcg caccaagtcg cccttctctt gcggaagaat ctgggcatag
     1381 taggcaacga ttcccaattt cttggcgatt tcaacggttg tttcctcccg atcgcccgaa
     1441 gccaaaaccg gctccaaccc cagagacttc aatcggacga cggctgagac cgcattcggt
     1501 ttcaacgtat ccgccaccgt caaagcgccg gccaccctac catccaccgc cacccagagg
     1561 aaactggcac ctgaattcgc agcaaattgt ttggcagaga ctccgtcaag tgagatccgt
     1621 tcacgcctca agagcgcttc attccctacc agaattcttt tcgcctccac cgtcgccacc
     1681 agaccaaaac ccggctcggc ggtgaaggcg gttggctcgc tcaaagcgaa tttcttctct
     1741 tcggcatagc gggtcaccgc ctccgccagc ggatgctcgg accgtttttc agccgaggcg
     1801 gcccagcgta ctacttgctc tggcgcaaac ccattcaaag ggaaaacggc ggttactgcc
     1861 ggtttgcctg aggtaacggt tccggttttg tcgaacacca ccgtgtctaa tttctgtgct
     1921 ttctccagcg ccccggcatc tttaatgaga attcccatct cagccccccg gccggtgccc
     1981 accataatcg ccgtcggggt ggccagcccc atggcgcagg ggcaggcaat gatcaaaact
     2041 gcaacgcagt tgagcaatgc gcgggtcagt ttgggctcgg gcccgaaaag aaaccaaacc
     2101 gcaaaagtga ttgcggcaat gaccagcacg atgggaacga acactgccgc cacccggtcg
     2161 gctaacttct gaatcggcgc tttggagccc tgcgcctttt ccaccaggtg aataatctgc
     2221 gccaggagtg tgtccttgcc aactttggtg gcccgaaaca cgaaactgcc agtcttatta
     2281 atcgttcccc cgatgacgcg gtccccgatt tttttctcca ccggcagact ctcgcccgtc
     2341 accattgact catcgatggc tgaatatccc tcgatcactt gtccgtctac cggaatccgt
     2401 tccccggggc ggacaatgac ggtatcatcg acgaatacct gatcgatcgg taggtccatc
     2461 tcggaaccat cccgccgcac gcgggcggtt ttgggagcaa gtccgaggag ttttttgatg
     2521 gccgccgagg tgcggccttt ggcctgggtc tcgagatacc gtcctaaaag aattaaagcg
     2581 attatagccg cagcggtttc gtagtaaacc tcggcccggt gcccggcaga ggtgaatccg
     2641 gaaggggaaa atgtcaccgc aacgctatag aagtacgccg ccgaggtacc gatggcgatg
     2701 agcgtgttca tatcggcgcg accgtgcttc aactgcgccc agaaaccccg atagaagcgc
     2761 gccccgg
//
LOCUS       DBNGZD010000426         2621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_415215, whole genome shotgun sequence.
ACCESSION   DBNGZD010000426 DBNGZD010000000
VERSION     DBNGZD010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_415215"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..88)
                     /locus_tag="AC16GM_11040"
                     /pseudo
     CDS             complement(1..88)
                     /locus_tag="AC16GM_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            84..1064
                     /gene="plsX"
                     /locus_tag="AC16GM_11045"
     CDS             84..1064
                     /gene="plsX"
                     /locus_tag="AC16GM_11045"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR2924243.1"
                     /translation="MAGAVLALREKTNGSRRSQPVPTELILVGEEKQIRRELGRLKAE
                     DLPIQIAHASEQVQMEEQATEGWKKKDSSIAVAMRLQKERQVQAVVSGGNTGAVMTTA
                     LLTLGRLQGVIRPAIASLFPTLRHPTVILDVGANADCKAQHLQQFGVMGSIYYSRLLK
                     RTEPRVGLLSIGEEKSKGNELVLASHRLLAETPVNFIGNVEGRDILRGQCEVVVTDGF
                     TGNVMLKFAESVYGFLASKFRDQIRSNVFSKVGVFLLGPFLRRMKRTLDYAEYGGAPL
                     LGIDGVCIICHGKSSPLAIKNAIKVAEQMVTEKVNEQIKEQLAAHALVAT"
     gene            1168..2118
                     /gene="fabD"
                     /locus_tag="AC16GM_11050"
     CDS             1168..2118
                     /gene="fabD"
                     /locus_tag="AC16GM_11050"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR2924244.1"
                     /translation="MKRALLFPGQGSQYVGMARDWYEAYPEVQNLFAQAEEILGYDLA
                     GICFAGPEERLTQTQHTQPAIFAHSTAAFTVLQSAGLTFDYVAGHSLGEYSALVANGC
                     LSFHDGLSAVKFRSRFMQEACDEKPGTMAAVIGLPADVVVGICREVSNFGRIQPANFN
                     SPEQIAISGERPGVERAVGLAKEKGALRAMLLPVGGAFHYHLMAPAAERLGEVLTTLS
                     FHPAKVPVVANVTAQPTEDPVLIRKLLVDQITCPVRWVETLQFLHQAGVTEFVEIGPG
                     RVLTGLVRRTLKGVATHSIDTTGDWAGLGAKLSQAALTEK"
     gene            2132..>2621
                     /locus_tag="AC16GM_11055"
     CDS             2132..>2621
                     /locus_tag="AC16GM_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2924245.1"
                     /translation="MRLRGKVAIVTGASRGIGKAIALRLAAEGADVVVAATNVQLIEE
                     TAHEIEAKGVRAKAVRVDVTRAEETKRLVEETSEAFGRVDILVNNAGITKDGLLVRLA
                     EADWDQVLAVNLKGAFLVSKACARVMMRQKSGTIINISSVVGLMGNPGQVNYVAAKAG
                     LIG"
BASE COUNT          545 a          741 c          850 g          485 t
ORIGIN      
        1 tgatgtcggg tcgggccacg accacctggc gatcaaaccg tccgggccgc agcaaggccg
       61 gatccaggac atcgggccgg ttggtggccg gagcggtttt ggccttgcgc gaaaaaacca
      121 acggcagccg gcgttcgcag cctgtaccta ccgaactcat cctagtcggt gaggagaagc
      181 aaattcggcg cgaactgggg cgcctgaagg cggaggacct ccccattcag attgcccatg
      241 cgtccgaaca ggtccagatg gaggagcaag ccaccgaagg ctggaagaaa aaagattctt
      301 ccatcgcggt ggccatgcgc ctgcagaaag agcgtcaagt ccaggcagtg gtctccggcg
      361 gcaataccgg cgcggtgatg acgaccgcac ttttgacgct ggggcggctg caaggggtga
      421 tccggccggc gatagcatcg ctttttccga ccctccggca cccgacggtg attttagacg
      481 tgggagccaa tgccgactgc aaggcgcagc acctgcagca gttcggcgtc atggggagca
      541 tctactacag ccgcctgctc aagcgcacgg agccgcgggt gggacttttg tccattggtg
      601 aggagaaaag caagggaaat gaattggtcc tggcctcgca tcgactctta gcagaaacac
      661 cggtcaactt catcggcaac gtcgaggggc gcgacatcct gcggggccag tgcgaggtgg
      721 tggtgaccga cggcttcacc ggcaacgtga tgctgaaatt cgccgaatcg gtctacgggt
      781 ttttggcgtc gaaattccgg gaccagatca ggagcaacgt cttttcgaag gtgggcgtct
      841 tcctcttggg gccttttctg cggcggatga agcggactct ggactacgcc gagtatggcg
      901 gggcgccgct gctgggaatc gacggcgtct gcatcatctg ccatggcaaa tcgtcgcctc
      961 tggccattaa aaacgccatc aaagttgccg agcagatggt gacagaaaag gtgaacgaac
     1021 agatcaaaga acagctggcc gcgcacgccc tggtggcgac ctgatgtcct ccggccattc
     1081 gccgaccgtt ctccgccgcc gaaggccagg tcgcttggca aagttaaacg tgaagtctct
     1141 cccagcattc agtaatttac aaatcccatg aaacgcgcac tcctgttccc cggccagggc
     1201 tcccagtacg tggggatggc ccgcgactgg tacgaggcgt atccagaggt ccaaaacctt
     1261 ttcgcgcagg cagaagagat attgggttac gacctggcag ggatttgttt tgccggtccg
     1321 gaagagcggc tcacccagac gcagcacacc cagccggcga ttttcgccca cagcaccgct
     1381 gccttcaccg tcctgcaatc tgcgggtctc acattcgatt acgtggcggg ccacagtctg
     1441 ggcgagtatt cggccctggt cgccaacggc tgtttatctt ttcacgacgg cctctccgcg
     1501 gtcaagtttc gctcacgctt catgcaggaa gcctgcgatg aaaaaccggg aacgatggcg
     1561 gcggtcatcg gcctgccggc agatgtggtg gtcgggattt gccgcgaggt ctcaaatttc
     1621 ggcaggattc agccggccaa tttcaattct ccggagcaga tagcgatttc cggtgaacgg
     1681 cccggcgtcg aacgggcggt gggtctggcc aaagaaaaag gggcgctgcg ggcaatgctt
     1741 ttgccggtgg ggggggcgtt tcattaccac ttgatggcgc cggcggccga gcgcctggga
     1801 gaagtcctga cgacactttc gtttcacccg gcgaaggtac cggtggtggc gaacgtcacc
     1861 gcgcaaccga ccgaagaccc cgtgctcatc cggaaacttt tagtcgatca aatcacctgc
     1921 ccggtacgct gggtggaaac cttgcaattc ctgcaccagg cgggtgtaac ggagttcgtc
     1981 gaaatcggac cggggagggt gctcaccggc ctggtgcgcc gcaccctcaa aggggttgcc
     2041 acccacagca tcgatacgac cggtgactgg gccggcctcg gcgccaaact ctcgcaagcg
     2101 gcgttgacag aaaaatgagg ggagcgtaag tttgcgcctg cgcgggaagg tggccatcgt
     2161 aaccggtgcc tcgcgcggca tcggcaaggc gattgccctg cggctggcgg ccgaaggtgc
     2221 cgacgtggtg gtggcggcca ccaacgttca actcattgaa gagaccgcgc acgaaataga
     2281 ggcgaaagga gtccgggcta aggcagtccg agttgacgtt acccgggccg aggagaccaa
     2341 acggctggta gaagaaacca gcgaggcctt tgggcgggtg gatattttgg tcaacaatgc
     2401 cggtattacg aaagacggcc ttttagtacg gctcgccgaa gccgactggg atcaggtgct
     2461 ggcagtcaac ctcaaagggg ccttcttggt gagcaaagcc tgcgcccggg tgatgatgcg
     2521 gcagaagagc ggcacgatca ttaacatcag ttcggtggtg ggtctcatgg ggaatcccgg
     2581 ccaggtcaat tatgtcgcgg cgaaagccgg cctgatcggc t
//
LOCUS       DBNGZD010000427         7143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1179061, whole genome shotgun sequence.
ACCESSION   DBNGZD010000427 DBNGZD010000000
VERSION     DBNGZD010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1179061"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="AC16GM_11060"
     CDS             complement(<1..908)
                     /locus_tag="AC16GM_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR2924246.1"
                     /translation="MTLENDALTYHRTGRPGKLEVVATKPFLTQRDLSLAYTPGVAEP
                     CRRIVADPQEVFDYTAKGNLVAVITNGTAVLGLGNIGPLAGKPVMEGKAVLFKRFADI
                     DVFDLEIAATDPDEFVRTVQLLEPTFGGINLEDVRAPECFRVEQELARTLSIPVFHDD
                     QHGTAIITSAGLLNALELVDKEIEKVKVVVVGAGAAGIACARLFISLGLKQEHLILVD
                     SKGVVYRGREVDMNPYKESLAADTKARTLADALVGADVLVGVSAPNLVTPPMLARMAP
                     KPIIFACANPDPEIPYPDAKKARPDA"
     gene            1136..1582
                     /locus_tag="AC16GM_11065"
     CDS             1136..1582
                     /locus_tag="AC16GM_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR2924247.1"
                     /translation="MIYSRGCEYAIRALAYMANQSGERFISAKEIGKKEGVPTFFLAK
                     TLQKLAQAGVLRSFKGPTGGFGLARSPKQITLYDIVEAIDGTGDLNRCAAGLVACSDA
                     APCPMHSQFKQLREHVQRYLKRQTVADLAKTVAEKKKLVSKSKSRS"
     gene            1872..2783
                     /locus_tag="AC16GM_11070"
     CDS             1872..2783
                     /locus_tag="AC16GM_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HIR2924248.1"
                     /translation="MRNRSSVWALGLAGLLALAFVGWALAGSRPKEISGENIGSEKCL
                     ACHEEVQAEYAHTVHFGVEENAQTELEKHMCEACHGPGAAHAEKPEDKSLHLTFKAKD
                     GESPDDWNRVCLQCHERGVQTHWAGSVHETRGVACVNCHTVMKNISREKQLAKETAPE
                     VCFTCHKMKKMQFQKSSHMPLVEGKVSCVDCHNPHGSPNPSMVIENSVVENCTKCHAE
                     KSGPFLWEHPPVRENCLNCHNPHGSNHDNLLIVKRPRLCQRCHVEARHPTTPQSEATL
                     FAIDRGCVNCHSQIHGSNHPSGMQFLR"
     gene            2797..4941
                     /locus_tag="AC16GM_11075"
     CDS             2797..4941
                     /locus_tag="AC16GM_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HIR2924249.1"
                     /translation="MKTLILFLATVTLAATAWTQEAVRTTGQLEGGFWQVLKNPGSAK
                     FQEYRQVKEDLLLNRFGLDLTKGNYYFGVNANELGKTDQGAVVRFGLVRKWKNNVSWS
                     QTPHLLSTTTREFFVNAGGGVFVVSDSIQRRLQSVPGSLKSFLEGAQPRNLFFQRNTA
                     SATTEFKPRSDITFKAGYTNERKQGSKQLGVTLFFNPVELPEPLNYETHRLSASAELA
                     RKNFSAAVGYSGRIFNNKTEVVIRDNLFRVTTDTATAPLRVRLALPVDNVAHNFTFTG
                     AYNLPKQTRSIATVSYGVATQNEPFLPHTINSAITDSGLALPAASLDGKFKTLLVNYQ
                     VSSRPVEKLSLKGKIRYYEFDNQTPSLDFGSYVAYGDYSLQTRGRENLPYGYIKRDYG
                     GEAGYQIVRQANASFAYTRENMTRNHREVLSSAEDIFKGTLDVRPSVWLTFRGSYSHA
                     ERKSQKYDSLFFEESFPEGEAVTNAGMSLNELRRFDVYNRNRDNVELSSQISPIDELD
                     VGLSFSLAQDDFANSAYGLEALWNRGWSMDISYSPTPRLTLLGDLGREDGRGEMESRY
                     RPVYANVGYDSLNNDWSGALRDRFENYGFGFSAEVWPKKLSWATNYGFTYSKSALLSS
                     FAPGGNPSGNAVSFPNVYNKFHRVASQVTYRLTSQTALKFGYLYEKYLETDWAIDPLQ
                     PFMAAASRSVFLGARVPGYEAHVVALKLSYNF"
     gene            5232..6479
                     /locus_tag="AC16GM_11080"
     CDS             5232..6479
                     /locus_tag="AC16GM_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924250.1"
                     /translation="MRRKMVITAATLVAFWAIWSFHNDLTAMTQFARKTGLECTSCHT
                     VIPKLTEMGYRYRAAGFRMPDEIQSKPEYKELGQFFSFRIQNRIDFKHKTASGKTTNQ
                     FQLTNHELTIYPATGAFAKQLASEVEVSWAADEPLEFENAYVRWVSGTEEGFWSTRLG
                     IFHPFEGYGASDRPVSLARPLFQVSSANYTQNTFFTLWEIDQFGLEAAYNLRRTTFAG
                     TVFNGLFFTGDGLEPAKGGGLQKPAGLRSQNTKDYQLVVNQLLTDNGGGVSAVYYRGT
                     VDLPITDTTFFQNNFQRAALYGSYPVTDKVVLLLGGALGMDQYYNPATGSTKGRFGSG
                     GGFGEIDLYAHPRATLAGRFDWFDPSDRKKNNEVWAATGAINVPFNNGFQLIWQYQHK
                     QTKVSSPPEKKEDNVQLRAIMIF"
     gene            6911..>7143
                     /locus_tag="AC16GM_11085"
     CDS             6911..>7143
                     /locus_tag="AC16GM_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR2924251.1"
                     /translation="MRNAKGLGLAVAGAILLAAAVSGTAQEKAKAPVFKYVGADKCKL
                     CHNTQAMGKIYDKWLASPHAKAYTTLASDSAKAA"
BASE COUNT         1630 a         1991 c         2052 g         1470 t
ORIGIN      
        1 atggcatcgg gccgggcctt tttcgcatcc gggtaaggga tttcgggatc ggggttggcg
       61 caggcgaaga tgatcggttt gggggccatc ctcgccagca tcgggggagt cacgaggttg
      121 ggggccgaaa cgccgaccaa aacgtcggcg cccaccagcg cgtcggccag cgtgcgggcc
      181 ttggtgtcgg cggctaagct ttctttgtag ggattcatgt ccacttccct gccgcggtag
      241 accacgccct tactatcgac caggatcaag tgctcttgct tgaggcccag agaaataaag
      301 agacgggcgc aggcgatccc cgccgcaccg gcgccgacga ccacaacttt cactttctca
      361 atttccttat ccaccaattc cagcgcgttc agcaaaccgg cggaggtgat gatggcggtg
      421 ccgtgctggt cgtcatggaa aaccgggatt gacagtgtcc gggccagttc ctgctcgacc
      481 cggaagcatt ccggggcacg gacgtcctct aagttaatgc cgccgaaagt cggctccaga
      541 agctgcaccg tgcggacaaa ctcatccgga tcggtcgccg cgatttcgag gtcgaagacg
      601 tcgatgtcgg cgaagcgctt gaagagcact gctttgcctt ccatcaccgg cttgccggcc
      661 agggggccga tattgcccag acccaggacg gcggtgccgt tggtaatcac ggcgaccaaa
      721 ttccccttcg cggtataatc aaacacttcc tgcgggtcgg ccacaatccg gcggcagggc
      781 tcggccacgc cgggggtgta ggcgagggag aggtcgcgct gggtcaaaaa cggcttggtg
      841 gcgacgacct ctaattttcc gggccggccg gtgcggtggt aggtcagggc gtcattttcg
      901 agtgtcatcg tttcttactt cctcacaaag gtgtgtgtgg gagcatcgcg tgcgcgggaa
      961 taatcatgcc cggcagtatt ataattgcgc caaaaccatc ttggcaatcg ttggtgaaaa
     1021 gcttttcagg tttcccgggc acttgacatc accacatcct tatacgtata atatattatg
     1081 ataaatgcct ccggtaatca aaattttggg ccgtccacct ggaacaagga gacttatgat
     1141 ctactcacgc ggatgtgagt acgccattcg ggcactggcc tacatggcca atcaatcggg
     1201 agagcggttc atttccgcca aagaaatcgg caaaaaggag ggtgtgccga ctttcttttt
     1261 ggccaagacc ttgcaaaagt tagcccaggc cggagtgctc cggtcgttca aagggcccac
     1321 cggcggcttc gggctcgccc gatcgcccaa gcagattacc ctctatgaca tcgtcgaggc
     1381 catcgacggc accggcgacc tcaaccgctg cgccgccggg ctagtggcct gttccgacgc
     1441 ggcaccctgt ccgatgcata gccagttcaa gcagttgcgg gagcacgtcc agcgctacct
     1501 caagcgccag acggtggccg acttagccaa aacggtggcg gagaaaaaaa agctggtctc
     1561 caagagcaaa tctcggtctt aagctaggct gaacgtgcta tcaaaacagg gctgttgcgt
     1621 tcagcgtccg agggttctcg aatacccatc tgcgcccatt ctccaatggg ataagaaaag
     1681 tcccgctgac tggggggcgg ggggcagcaa accggccctt gacaggggga aatgcgctgc
     1741 gcattatatt ccaaaatggt cttggtcatg gagaattcta accggtaata tttcatacca
     1801 aaaggtcgat aatgtaattg aggatgtctg acgtgtggga caggaaggga agcgtgctga
     1861 gggagggagc catgcgaaat aggtcttcgg tttgggcgct cggcttggcc gggctcctgg
     1921 ctctggcgtt cgtcggctgg gcgctggccg ggtcgcggcc caaggagatt tccggggaaa
     1981 acatcggcag tgaaaagtgt ctcgcttgcc atgaggaagt ccaggcggag tatgcgcaca
     2041 ccgtccattt tggcgtggaa gagaacgcgc agaccgagtt agagaagcat atgtgcgaag
     2101 cctgccacgg ccccggtgcg gcccacgcgg agaagccgga ggataagagt ttgcacctca
     2161 cgttcaaggc caaagacggg gaatcgcccg acgattggaa tcgagtatgc ctgcagtgcc
     2221 acgagcgcgg cgtgcagacg cattgggccg ggtcggtgca cgagacgcgc ggggtggcct
     2281 gcgtcaactg ccataccgtg atgaaaaata tctccaggga aaaacagctg gccaaggaaa
     2341 cggcgccgga ggtttgtttt acctgccaca agatgaaaaa gatgcagttc cagaaatctt
     2401 cccatatgcc attggtggaa ggcaaggttt cttgcgtgga ctgccacaat ccgcacggct
     2461 cccccaatcc cagcatggtt atcgaaaact cggtggtcga gaactgcacc aaatgccacg
     2521 ccgaaaaaag cggccccttt ttgtgggagc atccgccggt gcgggagaac tgtttgaact
     2581 gccacaaccc gcacggctcc aatcacgaca acctgctgat agtcaagcgc ccgcggctct
     2641 gccagcgctg ccacgttgag gccaggcacc cgaccactcc ccagtccgag gccacactct
     2701 ttgccatcga tcgcggctgc gtcaattgtc attcacaaat tcacggttcc aaccatccct
     2761 ccgggatgca atttctacga tagggaggcg aaggcgatga aaactttgat cctgtttctc
     2821 gcgacggtta ctctggcggc gacggcctgg acacaggaag cagttcggac caccggccag
     2881 ctggaggggg gattctggca ggttctcaaa aaccccggct ccgccaaatt tcaggaatac
     2941 cggcaggtta aagaggatct gctgttgaac cgcttcggcc ttgacctcac caagggcaac
     3001 tattacttcg gggtcaacgc gaacgaactg gggaaaaccg accagggagc ggtggtcaga
     3061 tttggactgg tccggaaatg gaagaataat gtttcctggt cgcagacccc gcatcttttg
     3121 tccaccacca cgcgagagtt cttcgtcaat gccggcgggg gcgtctttgt cgtttccgac
     3181 agcattcaga gaagactgca gtccgttccc ggcagtctga agagcttcct cgagggcgct
     3241 cagccgagaa acctcttctt ccagcgcaac acggccagtg ccacgactga attcaaaccc
     3301 cgctcggata taacctttaa ggccggttac accaacgaaa gaaaacaggg gagcaagcaa
     3361 ctgggagtta ctcttttctt caatccggtg gaactcccgg agcctttgaa ctacgaaacc
     3421 catcgccttt ccgcctcggc ggaactggcg cgcaagaact tttcggcggc ggtggggtac
     3481 agcgggcgga ttttcaacaa caagaccgag gttgtgattc gggataacct cttccgggtg
     3541 acgaccgata cggcaaccgc ccccctacgc gtgcggttgg cgctgcctgt ggacaatgtg
     3601 gcgcacaact tcacttttac gggcgcctac aatcttccca aacagacacg cagcatcgcg
     3661 acggtaagct acggggtggc cacgcagaac gagccctttt tgccgcacac catcaacagc
     3721 gccattacgg acagcggcct ggccttgccg gccgccagtc tggatgggaa attcaagacc
     3781 ctgctggtca actaccaggt cagttcccgg ccggtcgaaa aactttcgct gaaaggaaag
     3841 attcgctact acgagttcga caaccagacc cccagcttgg actttggcag ctatgtcgcg
     3901 tacggcgact acagtttgca aacccgcggg cgtgaaaacc ttccctacgg ttatattaag
     3961 cgggattatg gcggagaagc cggctatcaa atcgtccggc aggcgaacgc gagcttcgcc
     4021 tacacccgcg aaaatatgac ccgcaatcat cgggaagtgc tatcatcggc ggaggatatc
     4081 ttcaagggga ccctggatgt ccgcccgtcc gtctggctca ctttccgcgg ctcttattcc
     4141 cacgcggaac ggaaaagcca aaaatacgat tcgcttttct ttgaggagtc tttcccggag
     4201 ggggaggccg ttaccaatgc cggcatgtct ttgaacgaat tgcgccgctt tgacgtgtac
     4261 aaccgcaacc gggacaacgt cgagctctcc agccaaattt ctcccataga cgagttggat
     4321 gttggattaa gcttcagctt ggcgcaggat gactttgcca attcggcata cggtttggaa
     4381 gccctttgga accgaggctg gagcatggac atttcctact ctcccacccc ccgtctaacc
     4441 ttgttgggag acctcgggcg ggaagacggg cggggagaaa tggagtcccg ctaccgcccg
     4501 gtctatgcca acgtcggcta cgactccctg aacaatgact ggagcggcgc tttgcgagac
     4561 cgcttcgaaa actacggctt tggattctcc gccgaggtct ggcccaaaaa gctctcctgg
     4621 gcaacgaatt acggcttcac ctacagcaaa agcgccctgc tctcttcgtt tgctcccggc
     4681 gggaacccca gcggcaacgc ggtcagtttc cccaacgtgt ataacaagtt ccatcgcgtc
     4741 gcttcgcagg tgacttatcg gctcaccagt caaaccgccc tgaaattcgg ttacctgtac
     4801 gagaaatacc ttgaaaccga ctgggcgatt gacccgctgc agccctttat ggctgccgcc
     4861 agccgctcgg tcttcttagg cgcacgagtg cccggctacg aggcgcacgt cgtggcgctc
     4921 aagttgagtt acaactttta gattttttga gggtttttct ggagtgtccg ggttgccata
     4981 aaagacccgg acactctttt ttcatccccc cgatttttat ttttccgttt tcagtaactt
     5041 atctattgat aggacattac cagccaattt gaagaggggc tttaatgtga aagttttcac
     5101 aaaaaagact tgactacctg aatttggtcc tatatatact tggcagaaag ttagggacta
     5161 aaaagatata gtaacctgat tacagaagag ggaaccagga aagcagcggc caaccaccaa
     5221 aggagggtac gatgcgccgt aagatggtca tcaccgcagc cacgctcgtc gctttttggg
     5281 cgatctggtc gttccacaac gacctgaccg ccatgaccca gttcgcgcgg aagaccgggc
     5341 ttgagtgcac cagctgccac acggtcatcc ccaagttaac ggagatgggg tatcgctacc
     5401 gagcagccgg ttttcgcatg ccggatgaaa tccaaagcaa gccggagtac aaagaattag
     5461 gccagttctt ctctttccgc attcaaaatc ggatcgattt caagcacaaa accgccagcg
     5521 ggaagaccac gaaccagttc cagttgacga accacgagct caccatttac ccggccacgg
     5581 gagctttcgc caaacagctg gcgagcgaag tggaggtgag ctgggcggcg gatgaacccc
     5641 tcgaatttga aaacgcctat gtccgttggg tctcgggcac cgaggagggg ttctggtcca
     5701 cgcggttggg aatcttccat ccctttgagg gctacggtgc ctcggatcgg ccggtgagtc
     5761 tcgcccgacc cctgttccaa gtctccagcg ccaactacac gcagaatacg ttctttacgc
     5821 tctgggagat cgatcagttc ggtctggagg cggcgtacaa tctaaggcga acgaccttcg
     5881 cggggacagt tttcaacggt cttttcttta cgggtgacgg cctggagccg gcgaagggag
     5941 gcgggttgca gaaacccgcc ggccttcggt cgcagaacac gaaagattac cagctggtgg
     6001 tgaatcaact cttaactgac aatggcggtg gcgtgagcgc tgtgtactac cggggcacgg
     6061 tcgatttgcc catcaccgat actactttct tccagaacaa cttccagcgg gcggcgctct
     6121 acggatcgta cccggtcacc gataaggtcg tgctgctttt aggcggcgct ctggggatgg
     6181 atcagtatta caatcccgcc accggcagca cgaaggggcg gttcggcagc ggcggtggct
     6241 ttggagagat cgatctctac gcccacccgc gcgccaccct ggcgggtcgt ttcgactggt
     6301 tcgatccgtc ggatcggaaa aagaataacg aggtctgggc cgccaccggc gccatcaacg
     6361 tgccgttcaa caatggcttc cagttgatct ggcaatatca gcacaaacaa accaaagtca
     6421 gcagccctcc ggaaaaaaag gaggacaacg ttcagctacg agcaattatg attttctaag
     6481 ccggcgacgc gggacgacga cgacgttctc ccgcgtttcc tgtttgtttc cgggcattcg
     6541 cttctaagca ctatgactga tagggcgctc cgagaagttc aaatgaggga tagaggatga
     6601 tttttgtctt ttgataattt ttgcgacccg gcgtcggaac cggctaacaa gttggttacc
     6661 agtcggttgc ggcagttgta aaatacgaga aaagcaatgt gaagtttttc acaaaagcac
     6721 ttgacgagtc cgaccgtccc gcgtatactt tgttagatat gattataatc agatgtaaat
     6781 gtccggtaaa taatcgcctg accggcgacc gaaggcaggc cagagatcgg gttgtttcga
     6841 acaacaggcg acgaggtaac tctatcaacg acggagtaaa cgttcacgca ctgggcaggg
     6901 aggagcaggc atgagaaacg caaaagggct gggactggcg gtggcgggag ccatactttt
     6961 ggccgcggcc gtcagcggga ccgcgcagga gaaggccaaa gcaccagtat tcaagtacgt
     7021 cggtgccgac aagtgcaagc tgtgccacaa cacacaggcg atggggaaga tctacgataa
     7081 gtggctggcc tcgccccacg ccaaggccta cacaaccctg gcgtccgact cggccaaagc
     7141 ggc
//
LOCUS       DBNGZD010000428        14399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1248154, whole genome shotgun sequence.
ACCESSION   DBNGZD010000428 DBNGZD010000000
VERSION     DBNGZD010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 14399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14399
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1248154"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="AC16GM_11090"
     CDS             complement(<1..478)
                     /locus_tag="AC16GM_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924252.1"
                     /translation="MSNRNLWVASGIAGVLAVVSYILAITLPWPETRLGTSTALVVVT
                     AWPILSIVYSYGLYSFIAAERESIANRLAFLFAVAGFTTVLAMIVVQLAIGAGIGEIT
                     KGLDEQTSNALRRGLRLVDHGLDVAWDMLIGTALVFSGAAMRRRGGLGPGWAFPSAV"
     gene            802..2610
                     /locus_tag="AC16GM_11095"
     CDS             802..2610
                     /locus_tag="AC16GM_11095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR2924253.1"
                     /translation="MSKSTETGKVKKPSGVSSLSQRVPVSPPVPEKLESKIPPTGLPD
                     AILHEMEQTLQKIEAARPRLAGDKEGGKYRLADLEALLLINRVINSSLILDEILQIVM
                     QKAIELLKAERGFLMLVDERGELQFKTAHNIGKELLTDEDFRISRTIANQVARSGESV
                     YTSDALKDDRYAHQKSVMELHLRSIMCVPLKSRDAILGVLYLDNSSEVNVFLQSDLFL
                     FELFAGQAAFAIEHAKLYDSLLRMERYHEEVVNRTPVGIIVVDSAFTITTANEVAQQI
                     LGASGTLSGSLLEKKIDLFTLFPSGEREKWRALCQKVLATGQAHAEPRYYLHAGPDER
                     VLSLKISPLVRPETGTVGLILVFEETTEKVMLENYVIMSEKLVAKGEMAAAIGHELNN
                     YLTIISNCTEMLELTVRKGDQEKLQQNTGVILENIGKMKRFTDGLMDYTRLETEPVPY
                     ELPRLVDDLLFAIKPQQQFRKIRFVTEVAPGLPKVAMDVGQVQQVLLNLFNNAAEAMA
                     ERNPPGGTITIEVKLLPEKSQVEIVVADDGPGIQPAYLDRIFDPRFTTKKTGHGLGLS
                     TCKRIIENHRGTITVKSAPGVGTQFFIRLPLSQTQT"
     gene            2626..6435
                     /locus_tag="AC16GM_11100"
     CDS             2626..6435
                     /locus_tag="AC16GM_11100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR2924254.1"
                     /translation="MSRTTQVQPQPVVQKRVLSSRPRFLAGCRFRILATLGEGGFGAV
                     FGCYDRLRQKRVALKTCASRGAPGRPEANVKAGERFRREFHLARTLSHPGVVRAFDFG
                     VLDSGELYYTMDWCAGKPLSSFAPLEKLDFLPDLIRKICGVLEFLHAFHIVHSDLKSS
                     NILVRFDPQAPQGPWCDLKLMDFGLAEEFTFGAPQTQAGTLEYMAPEMIEGKGGDARA
                     DLYSLGCVLYELVTGRTPFASVDPLSVVEGHLAKAPASPAELRSELSPLLNDLILKLL
                     AKNPAERMQSAREVAAQVVPIPVAIRAPEPFAALRNYMESGLALALGKNTPALVTASA
                     KRGVVGIAYGGRTTILGPPPSDTLEMQLQAQGFATVTLDAAVPQPEIARALVKKLWLV
                     PGAHDGQRSAPQPTHLRHWLRVSEAGRGDAGFSERALEELTNWITAQMSAAGKISPPA
                     VIVQNVSQGFLETAVYRKVLTAIAHSGHCLVILCPGKSERYADDRRLWRGLNSGATAE
                     TWEFPRMGGGDFNRSTLVPETVIAPSDLSRLWEIFGREENIFAAGLRFLHEKNFLESC
                     DGLWRVNRQKMEGFRLPRPLRRSLIRQVSHLNPEQCALLRAGSIFSGPFTALGVARLT
                     GLTRPVAEKQVATLQKERVLNLVTGSETSVQHYGFGSALLRRALYESISASQRRAGHR
                     KVLKFLISIPDSTPEGQKSLADHALASDDATEAVTRVLAYAGLCVEKNEREETLLYFA
                     RAERKARRVRESNLRKQLLARVLFERADRYKGWGDFEPAEKDFRIVLSLIPVERDPSL
                     HAEVYRKLADLYRWQNRLDDAGRTIEQAIALLQKTGDRVALSRAYNNLGIITWRKGDV
                     PQSVAYYETAYQIQKEQGLAQEAILTLNNLGIAYIQTHRFSTALKHYAEALELGRREG
                     TPETIAHLLNNIGFTHASVGSLAEGKPYFTESLSLRRALGKKSEILLTLINLSWLAYL
                     TGDPDGLKQLGQEAIALAQEVDDPQSVAEASFVLAEESLREGDYAGAYALVQAPQMDA
                     VDTFISNPVARAAFWVRFYAGQGDCEGAESAAARAKEVAERSGDLRNKVLSQIAGLTL
                     ALAAENGWEESAALLREAERTIEPFEGLLEKSQLQLCKVRHALRSGAPALLPDELAKL
                     DTLLAQCESAPLLAERFFLEGKWRSRRRDFLKATQAFTQANNFAGQAGYRELLWRIGT
                     EAGRVAATIGDYETAFREYRSAYEIVKVIYSCLSDPAYQVRFIADPMVVTLVTEMKKL
                     AGRLVAK"
     gene            complement(7146..9572)
                     /gene="lon"
                     /locus_tag="AC16GM_11105"
     CDS             complement(7146..9572)
                     /gene="lon"
                     /locus_tag="AC16GM_11105"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR2924255.1"
                     /translation="MPLVKEPVKTEEPKIKLDSLPVLPTRGIVVFPAFLTPLLITEQR
                     YAKMIDEALMDGQPIGLFAQKNAEAENPTPNDLYGVGTAGLILKMLRFPDGSVRFLVQ
                     GVSRIRIKRVERTEPFLVVRAEEIPEKLEPSVEVEALVRNVLELLKKVVSLAPYLPED
                     LQISALNTEDPAKLADLIGNNLNITLEQKQDLLETSDVKERLRKVLVYLNKEVEVLEL
                     SRKIQTEAASELGKTQKEYILREQLKAIQKELGEGDERTQELEEFRKKIETGGLPELA
                     KEAAQRELDRLSRMHPSAAEYTVSRTYVDWLVNLPWIKSTQDVLDIPGAQKILDEDHY
                     DLEKVKERMLEYLAVRKLKAETKGPILCFVGPPGVGKTSLGRSIARALGRKFYRISLG
                     GMHDEAEIRGHRRTYIGALPGRILQGIRRADSNNPVFMLDEIDKIGQDFRGDPAAALL
                     EVLDPEQNNTFSDHYLDVPFDLSKVMFITTANILDPIPPVLRDRMEVIELPGYTDLEK
                     LMIAKKYLLPRELENHGLKPEQLAITDEAIKHVINDYTRESGLRNLDRELATICRKTA
                     KFIAAGEKEKVKVTPDTLHEFLGPVKFLQELATRTAQIGVVPGLAWTPSGGDILFVEA
                     TKMRGKNNLTLTGHLGDVMRESAQAALSYVRSSAGKLGLPEDIFEKYDVHLHVPQGAI
                     PKDGPSAGITMATALASLFTERSVKPQVAMTGEITLRGLVLPIGGLKEKALAAYRAGI
                     KTVILPKQNEKDLEEIPEEIRKNVQFIFVEKVEQVFDYVLNSAPVTRKGNQIPRLPDV
                     GVNQIAAQKR"
     gene            complement(9613..10065)
                     /locus_tag="AC16GM_11110"
     CDS             complement(9613..10065)
                     /locus_tag="AC16GM_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR2924256.1"
                     /translation="MPEERKDIFRDIRHIQEEMDLLFDHFYKIRTSPILTSHRLWRPH
                     IDVFNLNKEMVVICELAGVQESEVAIALAHNILTIRGERAQKKFAERAACHNCEIPTG
                     KFERNVHLPEPGDPEKVAALLHDGMLEIRVGKLPQNREKSRTVEIEPE"
     gene            complement(10139..10933)
                     /locus_tag="AC16GM_11115"
     CDS             complement(10139..10933)
                     /locus_tag="AC16GM_11115"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR2924257.1"
                     /translation="MRGKQTSHLLAIDIGNTHTVIGVFAGERLRAHFRLASNHALTAD
                     ECGILVTGLLANHAKRPIKPGGICLASVVPPLTPIYEDMAQKYFGVQPVTVSAAMKLG
                     IKVRTTDPRQVGADRLANAVAAYHLYGGPTIVVDLGTATTFDAISQQGEYLGGAIAPG
                     IETSATDLFRRAAQLFSVRLTRPARAIGKTTEESLLAGIVYGTAGQVDFLVRKLEAEL
                     GRQAAVVATGGLADLVVKDSETIQKVHPTLTLEGLRLIYARNRGRA"
     gene            11101..12711
                     /locus_tag="AC16GM_11120"
     CDS             11101..12711
                     /locus_tag="AC16GM_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2924258.1"
                     /translation="MWKKKSTAAFVTVAVSILLISIAFAQRPFTVKEKQLYYGIKAVA
                     APEQFKQFEKLSGRERAEWLRMFWAPKDPTPTTPENEFYEEHQRRVAHAVEIFCTARP
                     GPPWDDRGEIYIRYGEPDERNFRVERHYSRQMAAHSAYELRDVISDPLKYRSLVAHRL
                     ANPERDFGGDISDEPSASIARGSFTLRPRDEDLLRDPKAEFLSTRLEFAAVSGEVWRY
                     YRYGLTFQFQDEQNLGYFGLVPYIDAFGTAQDYQQFVRQAVVSLDVQKEIYQHDYGGK
                     AMDYAFTVIRFRAPNKVNYVVDVNLGLPLEKLGVGGDSVTGEKVECMRRIVLYDENYQ
                     PLATDSTTLAAPVSADSRKGRLLLEQKRYELKPGRYILAVEVRDLATQKIGIYKKELL
                     LPEYIAPDVQEISDVELASHIRPANEWDKKYRHKELVVMPQPSRVFFPDQTLQYYFEV
                     YNLKKDKDGKARFSVTPEVYGYRTRKVEYSFNPRDLEADTTDIYQTGKLNLRDLTPGE
                     YILSLKVKDRITGKEKTALAGFKVAKSE"
     gene            12876..13445
                     /locus_tag="AC16GM_11125"
     CDS             12876..13445
                     /locus_tag="AC16GM_11125"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR2924259.1"
                     /translation="MSNGTGLTKKQMVMFAVLIGVAVFLGVYFGIYFGSRGLGRHGGE
                     APVEAKFKANDPLPEVAVLDVNGTLEPLSKRISGKGAVVMFMSLGCHPCQQESEKWQK
                     ILAEKHPEEFAVLGVCYDKTEQIQAYLKEHGFTFPIFCDSARLFMRDWSVAQFPTVAV
                     VNREGKIAKVYSGFHPDFDDKEVKKVLKT"
     gene            13498..>14399
                     /locus_tag="AC16GM_11130"
     CDS             13498..>14399
                     /locus_tag="AC16GM_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013510.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2924260.1"
                     /translation="MLRGWRIYSAAMRSALVFFFCVWIGSPALSTPSPSRRVSKSRVS
                     DESATTGPEQTRQKLVRIAVLEDQGWLGYGQLLRYLKDAEPVVRQRAARSCGIIQDSL
                     ACPVLIQALKDRDSTVRQEAAFALGQIQCREAKGALLKATRDRSPTVRQLALEALGKL
                     GDTTVVDRIAKFLSDPSSALRGEAALALARLKSHQAIAPLARAARDWDPAVRWKAVYA
                     LFRQNDSVTIPILEAKFTDNDPYVRAFAARGLGQLHASAPAMVPLLSDPDWRVQVQAV
                     RALGQSKASESLLFFLEYDRREGTG"
BASE COUNT         2990 a         4121 c         4323 g         2965 t
ORIGIN      
        1 gaaccgccga ggggaatgcc cagccgggtc ctaatccgcc gcggcgccgc atcgccgcgc
       61 ccgagaagac cagggcggtg ccgattagca tgtcccaagc aacatcgagc ccgtggtcca
      121 ccagccgcag cccccgccgc agcgcattgg atgtctgctc gtcgagcccc ttcgtaatct
      181 cgccgatccc tgcgccgatg gcaagctgca cgacgatcat cgccagaacc gtcgtgaacc
      241 ccgcgaccgc aaagaggaac gccaagcgat tcgcaatgct ctcccgttcc gcagcaataa
      301 aactgtagag gccgtagcta tagacgatac tcaaaatcgg ccaggcagtc accacgacca
      361 atgcggtcga cgtcccaagc cgtgtctccg gccacgggag cgtgatggca agaatatagc
      421 tgaccacggc caaaaccccg gcaatcccgc tcgccaccca tagattacga tttgacacta
      481 ttcgcctctc ccgcaggctc cggtgattga acttccatct gccttgccaa taaaattacc
      541 atagccaatc cccaaaaagt tgtcaaggtg atggatttca gcaaatcgcc tccggcatga
      601 ggcgcaaaat acgccccttg ggattgtcgg acaaaggata gagccgtcca tccggcccct
      661 gccgcacggc ggtcttccca agtcggaatt taagtaactc tcgatctggc cgatacttaa
      721 agagatgagc gggaaaccat tgctccgcat ctgacggggc ggtggtctct gcgcgttcat
      781 ctttacgtgc atagggcgcc tatgagcaag tccactgaaa cagggaaagt aaagaagcct
      841 tcgggtgtga gctcgctttc ccagcgggtg cccgtctctc cgccggtacc tgagaaactc
      901 gagagcaaaa taccgccgac cggactgcca gacgccattc tgcacgaaat ggagcagacg
      961 ctccaaaaga ttgaggcggc ccggccgcgg ctggccggag acaaggaggg cgggaagtac
     1021 cgcttagccg acctcgaagc actgcttttg atcaaccggg tcatcaactc ctcgctcatc
     1081 ttagatgaaa tcctccagat cgtgatgcaa aaggcgatcg aactgctcaa ggccgagcgg
     1141 gggtttttga tgctcgtgga cgagcgcggg gagttgcagt tcaagaccgc ccacaacatc
     1201 ggcaaggaac ttttgaccga cgaggatttc cgaatttccc gcaccatcgc caaccaggtg
     1261 gcccgctcag gcgagtcggt ttacacctcg gacgccttga aggacgaccg ctacgcccac
     1321 cagaagagcg tgatggaatt gcacctgcgc tccatcatgt gcgtgccctt gaaaagccgc
     1381 gacgccatcc tgggagtcct ttacctggac aacagttcgg aagtgaacgt cttcctgcag
     1441 agtgacctct tccttttcga actctttgcc gggcaggcgg cttttgccat cgagcacgcc
     1501 aagctgtacg actcgcttct gcggatggaa cgctaccacg aagaagtggt gaaccgcacg
     1561 ccggtgggga tcatcgtggt ggacagcgcg ttcaccatca ccactgccaa cgaagtggcc
     1621 cagcagattc tgggagcatc gggaacacta tccgggtcgc ttctggagaa gaagatcgac
     1681 ctgttcaccc tcttcccgtc cggagaaagg gagaagtggc gggcgctctg ccagaaggtt
     1741 ctggccacgg ggcaagccca cgccgagccg cgctactatc tgcacgccgg ccccgacgag
     1801 cgggtcctct cgctcaaaat ttcgcctctg gtgcggcccg aaaccggcac ggtcggcttg
     1861 attttagtct ttgaagaaac caccgaaaag gtgatgctgg aaaactacgt catcatgtcc
     1921 gagaaattgg tggccaaagg ggagatggcg gcggccatcg gccacgagtt gaacaactac
     1981 ctgaccatca tctccaactg caccgagatg ctggagctga cggtcaggaa aggtgatcag
     2041 gaaaaactcc agcagaacac cggagtgatc ctggagaaca tcgggaagat gaagcgcttc
     2101 accgacgggc tcatggacta cacccggctg gagaccgagc cggtgccgta cgaactgccg
     2161 cggctggtgg atgacctcct gttcgccatc aagccgcagc agcagttccg aaagatccgt
     2221 ttcgtcaccg aagtcgctcc ggggttgccc aaggtagcga tggacgtggg gcaggtccag
     2281 caggtgcttt tgaatctctt caacaacgcg gcggaagcga tggcagagcg caacccgccc
     2341 ggagggacca tcaccatcga ggtgaaactt ttgccggaga agagccaggt ggaaattgtg
     2401 gtggctgacg acgggccggg cattcagccc gcgtacctgg accggatttt cgacccacgc
     2461 ttcaccacca agaaaaccgg ccacggcctg gggctttcca cctgcaaacg gatcatcgag
     2521 aatcaccggg ggacgatcac ggtcaaaagc gcccccggcg tcgggacgca gtttttcatc
     2581 cggctgccgc tctcgcagac gcagacctaa ttttgggaca ggggaatgtc ccgtacaact
     2641 caagtccaac cgcaaccggt cgtccaaaag cgcgtgcttt ccagccggcc acgctttctc
     2701 gctgggtgtc gctttcggat actcgccacg ctgggagaag gtggtttcgg tgcggtcttt
     2761 ggctgctacg accggctccg acaaaagcgg gtggcgctga aaacctgcgc cagccgggga
     2821 gcgcccggcc ggccagaggc gaacgtcaaa gccggggagc gcttccggcg ggaattccac
     2881 ttagcccgca cactttcgca tcccggtgtg gtgcgggcct ttgatttcgg cgtgctagac
     2941 tccggcgagc tctactatac catggactgg tgcgccggaa agccgctttc ctctttcgcg
     3001 ccgcttgaga aattagattt tctgccggat ttgatccgga aaatctgcgg cgtcctggag
     3061 ttcttgcatg cgttccacat cgtccactcc gacctgaagt ccagcaacat cctggtccga
     3121 ttcgatccgc aggccccgca ggggccctgg tgtgatctca aactgatgga tttcggcctg
     3181 gccgaggaat tcacttttgg cgcgccgcaa acgcaggcgg gaactctgga gtacatggcg
     3241 ccggagatga tcgagggtaa aggcggcgat gcgcgggccg acctctactc tctgggctgc
     3301 gtgctctacg aactggtcac ggggcggacg ccttttgcga gcgtcgatcc gctctcagtc
     3361 gttgaaggac atctggccaa ggccccggcg tctccggcgg agttacgttc tgaactttct
     3421 cctctgctca atgacttgat tcttaaactt ttggccaaaa atccggccga gcgcatgcaa
     3481 tcggcccgcg aagtcgcagc gcaggtggtg ccgatcccag tagccatcag ggcaccggaa
     3541 cccttcgcgg cgttgaggaa ttatatggaa agcggcctgg ctctagcact gggtaaaaac
     3601 acgccagcgc tcgtcaccgc ttccgccaaa cggggtgtcg taggcatcgc ctacggtggc
     3661 cggacaacga ttctggggcc gccgcctagc gatacgcttg agatgcagct gcaagcccag
     3721 ggttttgcaa ctgtcacact cgacgccgca gtgccacagc cagaaatcgc aagggcgctg
     3781 gtcaaaaagc tctggctcgt ccccggcgca cacgacggac aacggagcgc accccaaccc
     3841 acacacctgc ggcactggct ccgggtgtcc gaggcaggaa ggggagatgc cggtttttca
     3901 gaacgggcgt tggaagaact gacgaactgg attaccgctc aaatgtccgc tgcgggtaag
     3961 atttcccctc ccgcggtcat agtccagaat gtgtcgcaag gattcctcga aacagcagta
     4021 taccgcaagg tgctcacagc gatcgctcat tcgggacatt gcctggtcat cctctgcccg
     4081 gggaaaagcg aacggtatgc cgatgaccgg cgactctgga gggggttgaa tagtggggca
     4141 actgccgaga cctgggagtt cccccgaatg ggcggcggtg attttaaccg atcaacactg
     4201 gttcccgaaa cggtcattgc cccgagtgat ttatcccggt tgtgggaaat ctttggcaga
     4261 gaggaaaata ttttcgcagc ggggctgcgc ttcttgcacg agaaaaactt tctggagtcg
     4321 tgcgacggac tatggcgggt caaccggcaa aagatggagg gctttcgctt accccgtcca
     4381 ctgcggcgca gtctgatacg gcaagtcagc cacctgaacc cggagcagtg tgcgctcctc
     4441 cgcgccgggt caattttcag tggacccttc actgcccttg gcgtcgcccg gctcaccggc
     4501 ctgacgcggc cggtggcgga aaagcaggtg gcaacactcc aaaaagagcg ggtcctgaac
     4561 ctggtgactg gaagcgagac cagtgttcaa cactacggct tcgggtcagc acttttgcgc
     4621 cgggcgctgt acgaaagcat ttcggcgagt caaagacggg ccgggcaccg caaggtgctc
     4681 aaattcctga tcagcatccc cgattcaact cccgaagggc aaaaaagcct ggccgaccac
     4741 gcgctggcga gcgacgacgc gacagaagcc gtcacccggg tgctcgccta cgccggactg
     4801 tgcgtggaaa aaaatgagcg cgaggaaacg ctcctctact tcgcccgtgc ggagcgcaag
     4861 gcgcggcggg tgcgggagtc aaacctgcgg aagcagctgc tcgcccgggt gcttttcgag
     4921 cgggccgacc gctacaaggg gtggggcgat tttgaaccgg cggaaaaaga tttccgcatc
     4981 gtactctccc tgattccggt tgagcgggac ccgtcgctcc acgctgaagt ctaccgaaag
     5041 ctggccgacc tctaccgctg gcagaatcgg ctggacgacg ccggccggac gattgagcaa
     5101 gcgattgcgc tcttacaaaa gaccggcgac agggtggcgc tttcccgggc gtacaacaac
     5161 ctcggcatca tcacctggcg caagggagat gtcccgcagt cggtggcgta ctacgaaacg
     5221 gcctaccaga tacagaaaga acaaggtctg gcgcaagaag cgattttgac cctgaacaat
     5281 ctggggatcg cctacattca gacgcaccgg ttctccaccg ccctcaaaca ctatgcggaa
     5341 gcgctggaat taggacgccg agagggcacg ccggaaacca tcgcccacct cttgaataac
     5401 atcggtttta cccacgcgtc ggtcggctca ttggcggagg ggaaaccgta cttcacagaa
     5461 tcgctttcgc tccggcgcgc cttggggaag aaaagtgaaa tactcttaac cctgatcaat
     5521 ctcagctggc tggcctacct taccggcgat ccggatggac tcaaacaact ggggcaagaa
     5581 gcaatcgcct tagcccaaga agtggatgat ccacagtcgg tggcggaggc gtcattcgtt
     5641 ctggcggagg agtccctgcg cgaaggcgac tacgccggag cgtatgcttt ggtccaagca
     5701 ccacagatgg acgcggtgga cacgttcatc tccaacccgg tggcgcgggc tgccttctgg
     5761 gtgcgttttt acgccggaca gggtgattgc gaaggggcgg agagcgcggc ggcaagggcg
     5821 aaagaggttg cggagcgatc cggcgacctc cggaataaag tcttaagcca gatcgccggg
     5881 ctcacgctgg ctctggccgc cgaaaatggt tgggaagaga gcgctgcgct cctgcgtgaa
     5941 gcggaacgaa cgatcgaacc gttcgaggga ttgctcgaga agagtcaact ccagttatgc
     6001 aaggtccgcc acgcgctccg ctccggcgca ccggcgctcc tgccggatga attggcaaaa
     6061 ttggacactt tgttagcgca gtgcgagtca gctccgcttc tggcggaacg tttctttctg
     6121 gaagggaagt ggcgcagccg gcgacgggat ttccttaagg cgacccaggc cttcactcag
     6181 gcaaataatt tcgccgggca agcagggtat cgggaactgc tctggcgcat cggaacagag
     6241 gcgggccgcg ttgccgccac catcggggat tacgaaaccg ccttccgcga ataccggtcg
     6301 gcctatgaaa tcgttaaggt gatctacagc tgcctttccg acccggccta ccaggtccgc
     6361 tttatagccg acccgatggt ggtgacgttg gtgaccgaga tgaaaaaatt agcggggcgg
     6421 ttggtggcca aataaaaagg ccgtcgttgt cgacggccct ccgtccttct ggaggaacct
     6481 cccttactct acgagcaggc cgccagcact ggggccgccg ggagccactt tcgcttcgag
     6541 ttcctcaaga tgcagttcat acatctgtgg ctcacctcct ctgtgttagg gttggacgga
     6601 cccccttttc caagggggtt tttcttctcg cttcccttcg agcgattaca aaggccaccg
     6661 tcgcttggat agaggtaaaa tacccaccac catcaccgga tgtcaagact ctcaatgaaa
     6721 aggccgttgt tgtcaacggc ctcccgtcca ttctggagga acttcctccc tattatccgc
     6781 cgcccgcact ggggccgccg ggagccactt tcgcttcgag ttcctcgaga cgcagttcat
     6841 acatctgtgg ctcacctcct ctgagctaga gttggacgga ccccctttct tcaatcaagg
     6901 gggttataat tcgctctttt ttgagcgggc agactcaccg ccccttctca ccagacagaa
     6961 aataataaat ggcgatagaa cctgtcaagt agttttttta agaaaagtga taaattacag
     7021 tttattcaca actcactatg gcacaattgg ttaacggaaa ttttcgtggt ggcctgtctt
     7081 agaaaaatcg tatgcgggtg atgaaagagg tcagattgag ggggccaaga aaaattgccg
     7141 gggggttacc gtttctgcgc cgcaatctga ttgaccccga catctggcag gcggggtatc
     7201 tggtttccct tccgggtaac cggggcactg ttcagcacat agtcaaaaac ttgttcgacc
     7261 ttctccacaa aaatgaactg gacattctta cggatttctt ccgggatctc ttctaaatcc
     7321 ttttcgttct gcttggggag gatcacggtt ttgatgcccg cgcggtaagc ggccagggcc
     7381 ttttccttca agccgccgat gggcagaacc agcccgcgca gcgtgatctc gccggtcatc
     7441 gccacctgag gcttcaccga cctttcggta aaaagcgaag ccagggcggt ggccatggtg
     7501 attcccgccg acgggccgtc tttgggaatt gcgccctgcg ggacgtggag gtggacgtcg
     7561 tacttctcaa agatgtcttc gggaaggccc agtttcccgg ccgaggagcg gacgtaggag
     7621 agcgccgcct gggccgactc gcgcatcacg tcgcccaaat gcccggtgag ggtcaggttg
     7681 tttttgccgc gcattttggt ggcttccaca aagagaatgt cgccgcctga gggggtccag
     7741 gccaggcccg ggacgacgcc gatctgcgcg gtgcgggtcg ccagctcctg aaggaatttc
     7801 accgggccca gaaactcgtg cagcgtatcc ggcgtcacct ttactttctc tttttccccg
     7861 gcggcgatga acttggcggt cttgcggcaa attgtggcca gctcccggtc caaattccgc
     7921 aagcccgatt cgcgcgtgta gtcgttaatg acgtgtttga tggcctcgtc ggtgatggcg
     7981 agttgctccg gttttaagcc gtgattttct aattcgcgcg gcaggaggta ctttttggcg
     8041 atcataagtt tctccaaatc ggtgtagccg gggagttcga tcacttccat ccggtcgcgc
     8101 aaaaccggcg ggatcgggtc cagaatattg gccgtggtaa tgaacatcac cttcgagagg
     8161 tcaaagggga cgtccaggta gtggtcggag aaggtgttgt tctgctccgg gtcgagcacc
     8221 tcaagcaggg ccgcggccgg gtcgccgcgg aagtcctggc cgattttatc gatttcgtcc
     8281 agcatgaaaa ccgggttgtt cgaatcggcc cggcggatgc cctggagaat ccggccgggg
     8341 agggcgccga tgtaggtgcg gcggtggccg cgtatttcgg cttcgtcgtg catgccgcct
     8401 aaggaaatgc ggtagaattt gcgtcccaga gcccgggcga ttgaccgtcc caaagacgtt
     8461 ttccccacgc ccggcggccc cacaaagcag aggatcggcc ctttggtttc ggctttcaac
     8521 ttccgcaccg ccaggtattc cagcatccgt tccttgactt tctccaagtc gtagtggtct
     8581 tcgtccaaaa ttttctgcgc gccggggatg tccaagacat cctgagtgct cttgatccag
     8641 gggaggttca ccagccagtc gacgtaggtg cgggagacgg tgtactcggc ggcggagggg
     8701 tgcatgcggc tcaggcggtc gagttcccgc tgggcagctt ccttggccag ttccggcagg
     8761 ccgccggttt caattttctt ccggaactct tctaactcct gcgtcctttc gtcgccttca
     8821 cccagctctt tctggatcgc tttcaactgc tcgcgcagga tgtactcttt ctgggtcttg
     8881 cccagttcgc tcgccgcctc ggtctggatt ttgcgcgaga gttccaggac ctccacctct
     8941 ttattgaggt agaccaacac cttgcgcagc cgttccttga cgtcggaggt ttccaagaga
     9001 tcctgcttct gttccagggt gatattgagg ttattgccga tcaggtcggc taatttggcg
     9061 gggtcctcgg tattcaaggc cgagatctgc aggtcctccg gcaggtaggg ggccagactc
     9121 accacttttt tcaaaagttc cagaacgttg cggaccagcg cttccacctc gactgagggc
     9181 tccagttttt cgggtatttc ctccgcccgg accaccaaga aaggttcggt ccgctctacc
     9241 cgcttgattc tgatgcggga gactccctgg actaagaagc ggaccgaccc gtcggggaaa
     9301 cgcagcattt tcaaaatcaa accggcggtg cccactccgt agaggtcgtt cggcgtcgga
     9361 ttttcggcct cggcgttctt ctgggcgaag agcccgatgg gctggccgtc catcagggcc
     9421 tcgtcgatca tcttggcgta gcgctgctcg gtgatcaaaa gcggggtgag gaaagccgga
     9481 aaaaccacga tcccccgcgt gggcaaaacc ggcagggagt cgagtttgat tttcggttct
     9541 tcggttttaa ccggctcttt gaccagtggc atccaccctc cggaaaattc gtatggtcag
     9601 gaccgctgtt gcctattccg gctcgatttc tacggttcgg gatttttctc gattctgggg
     9661 aagtttcccc acccgaattt cgagcatgcc gtcgtgcagg agggcggcca ctttttccgg
     9721 gtcgcccggc tccggcaggt ggacgttgcg ctcgaacttg ccggtgggaa tttcgcagtt
     9781 gtggcaggcg gcgcgttcgg caaacttctt ctgcgcccgc tcgccccgga tggtcaggat
     9841 gttgtgcgcc agcgcaatgg cgacctccga ctcctgcacc ccggccagtt cgcagatgac
     9901 caccatctct ttattcaggt tgaagacgtc aatgtgcggc cgccagaggc ggtggctggt
     9961 caaaatcggt gaggtgcgga tcttgtagaa atggtcaaag aggaggtcca tctcctcctg
    10021 aatgtggcgg atgtcccgga aaatgtcttt gcgttcttca ggcatctttc cctctttatg
    10081 tcgtcatttc cacaggtaat acgtactgtc cctcccgggt aagagtgcct tcaagtcgtc
    10141 aagcccgacc ccggttgcgg gcgtaaatca ggcgcagccc ctccagggtc aaggtggggt
    10201 ggactttctg tatggtctcc gaatctttaa cgaccagatc ggccaggccg ccggtggcga
    10261 cgactgcggc ctgccggccc agttccgctt cgagtttgcg cacgaggaaa tcgacctgtc
    10321 cggctgtgcc ataaacaata cccgccaaca gactctcctc ggtggttttg ccgatggcgc
    10381 gtgcgggccg ggtcaggcgc acggagaaca actgggccgc ccggcggaaa aggtcagtcg
    10441 ccgaggtttc gattcccggg gctatggcac cgcccaagta ttcgccttgc tgggagatgg
    10501 cgtcgaaggt cgtcgccgtt cctaaatcga cgacgatggt cggtccgccg taaaggtggt
    10561 aggcggccac cgcattggct aaccgatcgg cgccgacctg gcggggatcg gtcgttcgca
    10621 ctttgatgcc taacttcatt gcggcgctaa cggtgaccgg ctgcacgccg aagtacttct
    10681 gcgccatgtc ttcgtagatc ggggtcaaag gcggcacgac tgacgccaga cagatcccgc
    10741 cgggcttgat cggtctcttg gcatggtttg cgagcaggcc ggtgaccaga atgccgcact
    10801 catcggcggt taaagcatgg ttcgatgcca gccggaaatg cgcccgcagc cgttccccgg
    10861 caaagacgcc gatgacggtg tgggtgttgc cgatatcgat ggcgaggagg tgactggttt
    10921 gctttccgcg cacaaggggt aaagtatcca gaatgggccg acctgtcaaa atgcggtcag
    10981 atacccagct atttcaactt tattggctgg aaaatttcct gatgcgtata ttatcaatag
    11041 aggtatgtta cgttcagttg ggcgagacgg ttcgctcccg gtttgacgaa ggaaaaaacc
    11101 atgtggaaga aaaaatcaac cgcagctttc gttacggtcg cggtttctat tctactcatc
    11161 tccatcgcct tcgctcagcg gcctttcaca gtgaaagaaa agcaactcta ctacggtatt
    11221 aaggctgtgg ctgctcccga acagtttaag caatttgaaa agctatctgg aagggagcga
    11281 gcggaatggc tgcgaatgtt ctgggcaccc aaggatccca caccgaccac gccagaaaat
    11341 gaattttacg aagagcacca gcgccgcgta gctcacgcgg tagaaatttt ttgcactgca
    11401 cggccaggac cgccttggga tgaccggggc gagatttata ttcgctatgg ggaaccggat
    11461 gagcggaatt tccgcgtaga gcggcattac agtcgtcaga tggcagccca ctcggcctac
    11521 gagctccggg atgtgatcag cgatccgctc aagtaccggt cgcttgtagc tcatcggtta
    11581 gccaacccgg aaagagactt tggtggtgat atcagtgatg agccctctgc atcgatagcc
    11641 cgtggttcct tcaccctccg ccctcgcgat gaggatctcc tgagagatcc caaagccgaa
    11701 tttttaagca ctcgcctcga atttgcggca gtttcgggag aggtgtggcg gtactaccgc
    11761 tatggtttga cttttcaatt ccaggatgag cagaatttag ggtacttcgg actggtgccg
    11821 tacatcgacg ccttcggcac ggcgcaggac taccagcaat tcgtgcggca ggcggtggtg
    11881 tcgctcgatg tgcagaaaga gatttatcag cacgactacg gcggtaaggc gatggactat
    11941 gcttttacgg tgatccgctt ccgggcaccg aacaaggtga attacgtcgt ggacgtgaac
    12001 ctgggattgc cgttagagaa attgggtgtg gggggagact ccgtcaccgg cgaaaaagtt
    12061 gaatgtatgc gccggattgt gctttacgat gaaaactacc agccgctggc aaccgacagc
    12121 actacgctcg ctgcgccggt aagtgccgat agccgcaagg gacggttgct cttggagcag
    12181 aagcgctacg aactgaaacc gggcaggtat atcctggctg ttgaagtgcg cgatttggcc
    12241 acccagaaga tcggtatcta caaaaaagaa ctgctcttgc cggagtacat cgcaccggac
    12301 gtgcaggaga tttcggacgt ggagttggcg agccatattc gcccggccaa cgagtgggat
    12361 aaaaagtacc ggcacaaaga actggtggtg atgccccagc cctcgcgggt gttctttccc
    12421 gaccagacgt tgcagtatta ctttgaggtt tacaatctca aaaaggacaa ggacggcaag
    12481 gcgcggttct cagttacacc ggaagtgtac ggctaccgca cccgtaaggt ggagtacagc
    12541 ttcaacccgc gcgacttgga agccgacacg accgacatct accagaccgg caagctcaac
    12601 ttgcgcgatt taactcccgg agagtacatt ctctccctca aggtgaaaga tcggataacc
    12661 ggaaaggaga agacggcgct ggcggggttt aaggttgcaa agagcgaata gtcatcggtt
    12721 acgtaggcgc ggcgccctcg ccgcgctcaa tctgcgggtg gcgaccgggg gcggtcgccc
    12781 cacgcgggag aaaagattcg acgacgctct ggagaacgtc gctacacact taagatcaat
    12841 gggtgggtag gagtccaacg agcagaggag gcggaatgtc caacggcacg gggcttacca
    12901 aaaaacagat ggtgatgttt gcggttctga tcggcgtggc cgtgttctta ggcgtctact
    12961 tcggcattta cttcgggtcg cggggcctcg ggcggcacgg cggcgaagcg ccggtggagg
    13021 cgaaattcaa ggccaatgat ccgctgccag aagtggccgt cctcgacgtg aacgggacgc
    13081 ttgagccgct ttcgaaaagg atttccggaa aaggtgcagt agtgatgttc atgtctctgg
    13141 gttgccaccc ctgccagcag gaatcagaaa agtggcagaa gattctggcg gagaaacatc
    13201 ccgaggagtt tgccgtgctg ggcgtctgct acgataaaac cgagcaaatt caagcctacc
    13261 tgaaggagca cggctttacc ttccctattt tctgcgactc ggcgcggctt ttcatgcgcg
    13321 actggagcgt ggcgcagttc cccacggtgg ccgtcgtcaa tcgcgagggg aaaattgcga
    13381 aagtctactc cggtttccat cccgactttg acgataaaga agtgaagaag gttctgaaga
    13441 cgtaggttgc atcgggggcc ccgcaaaaag tcctccggcg agtgagggag gacttttttg
    13501 ttgcggggct ggcgcatata ttccgccgcc atgcgctcgg ctctcgtttt cttcttctgc
    13561 gtctggatcg gatcgccggc cctatccacg ccatctcctt ctcggcgagt ctcgaagagt
    13621 cgagtctccg acgagtctgc gacaaccggg ccggagcaaa cccgccagaa gctcgtccgc
    13681 attgccgtcc tcgaagacca gggctggctg gggtatggcc aacttttgcg ctacctgaag
    13741 gacgccgagc ccgtcgtgcg ccagcgggcg gcgcgctcct gcgggattat ccaggactcg
    13801 ctggcctgcc cggttttgat ccaagcgctc aaagatcggg attctaccgt gcggcaggaa
    13861 gccgcctttg ccctcggaca aattcagtgc cgggaggcca aaggggcact tttgaaggcg
    13921 acgcgggatc ggtcgccgac agtccggcag ctggcgctgg aagcgttggg gaaattgggc
    13981 gatacgacgg tcgttgaccg gattgccaaa ttcttgagcg accccagttc cgccctgcgc
    14041 ggggaagcgg ctctggcttt ggctcgacta aaaagtcacc aagccattgc accgctggcg
    14101 cgggccgcgc gggattggga ccccgccgta cgctggaaag cggtctacgc gctcttccgg
    14161 cagaacgatt cggtgaccat cccgatctta gaagcgaaat ttacggacaa cgacccctac
    14221 gtgcgggcct ttgcggcacg cgggctgggg cagttgcacg cttcggcccc ggcgatggtg
    14281 cccttgcttt ctgatcccga ctggcgcgta caggtgcagg cggtgcgcgc cctggggcag
    14341 agcaaggcgt ccgaaagtct gcttttcttt ttggagtatg accgccggga aggaacagg
//
LOCUS       DBNGZD010000429         3588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1248169, whole genome shotgun sequence.
ACCESSION   DBNGZD010000429 DBNGZD010000000
VERSION     DBNGZD010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1248169"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2562)
                     /gene="rpoC"
                     /locus_tag="AC16GM_11135"
     CDS             complement(<1..2562)
                     /gene="rpoC"
                     /locus_tag="AC16GM_11135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR2924261.1"
                     /translation="MADVTVRSSRRPSDFSAIKIQLASPEMIRQWSYGEVTKPETINY
                     RSFKPERDGLFCERIFGPVKDWECNCGKYKRIRFRGIVCDRCGVEVTQSKVRRERMGH
                     IELAVPVSHIWYFKSLPSRMAYLLDVSIRDLERILYYEAYVVIDPGNTSLKQSDIITE
                     EQYTQLEEAKKEFTAKMGADAIYEILKQIDIEELSISLRAQVKVETSVARKKDTLKRL
                     RVIEAFRQSRNRPEWMILQVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRVINRNNRL
                     KKLIDIKAPDVILRNEKRMLQEAVDALLDNGRRTQSVRGDSKRPLKSLSDLLKGKQGR
                     FRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKNMALELFKPFLIMKLEEKGYVQTVK
                     SAKKLVERERPEVWDILEEIISDHPVMLNRAPTLHRLGVQAFYPVLVEGKAIRLHPLV
                     CTAFNADFDGDQMAVHVPLSFEAQLEARVLMLSTHNLLSPSSGKPIVAPNQDIVLGCY
                     YLTKDRPGDKGEGMRFWGPEEVQAACNAGVIGLHARIKMKLDGDLVETTAGRVLFSSI
                     LPPELGFFNEVASKGKLEDLIGQAYKTLGAYRTAAFLDELKKLGFEYATVAGVTVGLD
                     DLVIPEEKEGLISRAEKEVERIRKQYERGLITNGERYNKVIDTWTHTSTEVAERMFAR
                     LEADRHGFNPVYMMADSGARGSKEQIRQLAGMRGLMAKPQKKITGQIGEIIESPIISN
                     FREGLTVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVIINEEDCGTILGL
                     SIGALKEGEEVIESLKDRVLGRVALEDVVDLITEEELARAGEEITETAAEQIEESG"
     gene            complement(2632..2979)
                     /locus_tag="AC16GM_11140"
     CDS             complement(2632..2979)
                     /locus_tag="AC16GM_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924262.1"
                     /translation="MWQFSNCGRTHRVLPVVMAIFLSGCCQLGLTCYELYIIGEPKLN
                     DADIYVDNKLVKSSVNGSATLHLSWETHEVKVVQKGFKPYEIVIEGKGGRSERGLKVK
                     FEPLNAVDTVHTP"
     gene            complement(3094..>3588)
                     /locus_tag="AC16GM_11145"
     CDS             complement(3094..>3588)
                     /locus_tag="AC16GM_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR2924263.1"
                     /translation="EKEIRSYLKEAADGQKKQGEEPLILEHGKTILRDGRTGEPFDQN
                     VTVGIMYMLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYG
                     AAYALQEMLTVKSDDVAGRSKIYEAIVKGENPPEPGVPESFNVLVKELQSLGMAVQLR
                     EVNK"
BASE COUNT          680 a         1105 c          995 g          808 t
ORIGIN      
        1 gccgctctcc tcgatctgct cggccgcggt ttcggtgatc tcttcaccgg cgcgggccag
       61 ttcttcttca gtgatcaggt ccaccacatc ttctaacgct acccggccca ggacccggtc
      121 tttgagcgac tcaatcactt cttcgccctc tttcagggcg ccgatggaaa gccccaggat
      181 cgtgccgcaa tcctcttcgt tgatgatcac gtcctgggcc acatccacga gccgccgggt
      241 gaggtagccg gcatcggcgg ttttaagcgc tgtgtcggcc agccctttgc gcgctccgtg
      301 ggtggagatg aagtactcca gaacagtcag cccttcgcgg aaattggaaa taattggcga
      361 ctcgatgatt tcgccgatct ggccggtaat tttcttctgc ggcttggcca tgagcccccg
      421 catgccggcc agctgccgga tttgctcctt ggagccgcgg gccccggaat cggccatcat
      481 gtagaccggg ttgaagccgt gccggtccgc ctccagccgg gcgaacatcc gctcggccac
      541 ttcggtcgag gtgtgggtcc aggtgtcgat gaccttgttg taccgttcgc cgttggtgat
      601 gagcccgcgc tcgtactgct tccggatccg ctcgacttcc ttttcggccc ggctgatcaa
      661 gccctccttc tcctccggaa tgactaagtc atcgaggccc accgtcacgc cggccacggt
      721 ggcgtactca aaacccagtt ttttcagttc atccaagaag gccgcggtgc ggtaggcccc
      781 cagcgtcttg taggcctggc cgattaaatc ctccaactta cccttgctcg ccacttcgtt
      841 gaagaaaccc aattccggcg ggagaatgga actaaagagc acccggccgg cggtcgtctc
      901 cacgaggtca ccgtcaagct tcatcttgat ccgcgcgtgc agaccgatga cgcctgcatt
      961 gcaggcggcc tggacttctt ccggacccca gaagcgcatc ccctcacctt tgtccccggg
     1021 gcggtctttg gtcaggtagt agcagcccaa aacgatgtcc tggttcggcg ccacgatcgg
     1081 cttgccgctg gaaggggaga ggagattgtg ggtcgagagc ataagcacgc gcgcttccaa
     1141 ctgcgcttca aacgagagcg gcacgtgcac cgccatctgg tcgccgtcga agtcggcgtt
     1201 gaacgcggtg caaaccaacg ggtgcaaacg aatcgctttg ccttcgacta acacggggta
     1261 gaaggcctga acgcccagcc ggtggagggt gggcgcccgg ttaagcatca ccgggtggtc
     1321 ggagatgatt tcctctaaaa tgtcccagac ctcgggccgc tcccgctcga ccaatttttt
     1381 ggccgatttg acggtttgga cgtacccctt ctcctctaat ttcatgatga ggaacggttt
     1441 gaagagctcc aaagccatgt tcttcggcag cccgcactgg tggagtttca gttccgggcc
     1501 cacgacgatg accgagcggc cggagtaatc gacccgcttg cccaaaaggt tctggcggaa
     1561 gcgcccctgc ttccctttca aaaggtcgga gagcgatttg agcggccgtt tggagtcgcc
     1621 gcggaccgac tgggtgcgcc ggccattgtc caggagtgcg tcgaccgctt cctgcaacat
     1681 ccgcttctca ttgcgcagga tgacgtccgg cgccttgatg tcgatcagtt ttttgagccg
     1741 gttgttccga ttgatcaccc ggcggtagag gtcgttcaag tcggaggtgg caaagcgtcc
     1801 gccttccaga ggcaccaagg gcctgagatc gggcgggatc accggcaaaa cctggaggat
     1861 catccactcc ggccggttcc gggactggcg aaaggcctcg atcacccgca ggcgcttcaa
     1921 agtgtccttc ttgcgcgcga cggaggtttc cactttcacc tgggcgcgca gggaaatcga
     1981 aagctcttcg atgtcgattt gcttcaggat ctcgtagatc gcatccgccc ccattttggc
     2041 cgtaaactcc tttttggctt cctccaactg ggtgtactgc tcctcggtaa tgatgtcgct
     2101 ctgcttgagg gaagtgttgc cggggtcgat gacgacgtag gcctcgtagt agagaatgcg
     2161 ttccaaatcg cgaatggata catcgagcag gtaggccatg cgcgagggga gcgacttgaa
     2221 gtaccagatg tgggagaccg gcaccgccag ttcgatgtgc cccatcctct cgcgccggac
     2281 tttggactgg gtgacctcca cgccgcagcg gtcgcagacg atgccgcgaa agcggatccg
     2341 tttgtacttg ccgcagttgc actcccaatc tttcaccggg ccgaagatcc gctcgcagaa
     2401 gagcccgtcc cgctccggct tgaacgagcg gtagttgatc gtctccggct tggtcacctc
     2461 gccgtaggac cactgccgga tcatctccgg cgaggccaat tgaatcttga tggccgagaa
     2521 atccgacggc cgtcgggaag aacggacagt tacgtcagcc acgcgtctcc ctccttaatt
     2581 tctttgaact caataagcat caaatacctt caaagttcta tggtttcacc atcagggtgt
     2641 atgcactgta tccaccgcat tgagcggttc gaacttaact ttaagccctc gctcacttcg
     2701 tccccctttt ccctcaataa cgatttcata cggcttaaac cctttttgca ccaccttaac
     2761 ttcatgcgtc tcccacgaaa gatgcaaagt agcactacca ttgacagagg atttaaccag
     2821 cttgttgtca acataaatat cagcgtcatt cagcttaggc tctccgatta tataaagttc
     2881 gtaacaggtc agcccgagtt gacaacaacc tgatagaaag attgccatca ccacgggcag
     2941 aacacgatga gttctcccgc aattagagaa ttgccacatt agttgctctt ggtaccagta
     3001 atctctaacc cgacgaagca ggtcaggcct gatgacctga cccacctctt tcgggctcaa
     3061 cctcgacact ccgatacttc gaaacctcga cacttattta ttcacttccc tcagctgcac
     3121 ggccatcccc agactctgga gttccttgac caagacgttg aacgactccg gcacgcccgg
     3181 ctcgggcggg ttttcgccct tgacgatggc ctcgtaaatt ttcgagcggc cggccacgtc
     3241 gtcggatttg accgtcagca tctcctggag cgcgtaggcc gcgccgtagg cctccagggc
     3301 ccagacctcc atttcgccga agcgctggcc gccgaactgc gccttgccgc ccaggggctg
     3361 ctgggtgaca agcgagtagg ggccgatcga gcgggcgtgg atcttgtcgt cgaccaggtg
     3421 gtggagcttg agcatgtaca tgatgcccac cgtgacgttc tgatcgaacg gttcgcccgt
     3481 ccggccgtcg cgcaggatgg tcttgccgtg ctccaggatg agcggctctt cgccctgctt
     3541 cttttgcccg tccgccgcct ctttcaaata gctgcggatt tccttttc
//
LOCUS       DBNGZD010000430         5185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_624518, whole genome shotgun sequence.
ACCESSION   DBNGZD010000430 DBNGZD010000000
VERSION     DBNGZD010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_624518"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="AC16GM_11150"
     CDS             complement(<1..260)
                     /locus_tag="AC16GM_11150"
                     /inference="COORDINATES: protein motif:HMM:NF016430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HIR2924264.1"
                     /translation="MNDAREMKRMDLSQEFRQGRDRGEGDRELFRAVQKGDQTAFRNL
                     VERYKDRLFNVVVRLLADREEASDVVQETFLRVFQHRERYNPA"
     gene            complement(630..860)
                     /locus_tag="AC16GM_11155"
     CDS             complement(630..860)
                     /locus_tag="AC16GM_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924265.1"
                     /translation="MGDLTIIAISMSVLALTWAVVRMNERVEDLQKKLRRISDQFNRD
                     TVGQRTPAAPRPMQAGSTYQGPMQPGQMQPVG"
     gene            1049..1705
                     /locus_tag="AC16GM_11160"
     CDS             1049..1705
                     /locus_tag="AC16GM_11160"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR2924266.1"
                     /translation="MYKASKRACDLFFATVALVLLSPFFVLAALAILFDSGRPVFFKQ
                     TRVGKDGVLFEMLKYRSMIKGAEQSGPRVTAPRDPRITRIGLILRKTKIDELPQLVNV
                     LKGEMSLVGPRPEVPRLASRYSAEQKKIFQVKPGLTSPGTLYYYRFQAHDHPEGVDLE
                     DYYLQKQLPEKLAYDFAYLNRPAIGKDLLLLLKTLWVIVTTPFVRPQAVESKGNIMPD
                     "
     gene            complement(1903..3198)
                     /locus_tag="AC16GM_11165"
     CDS             complement(1903..3198)
                     /locus_tag="AC16GM_11165"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR2924267.1"
                     /translation="MKKLLITPLVFLGLTSSGLTQPEQLGVFVEPSAANFFGFDARVM
                     AMGGAALANATSGSATNYNPAALARIHRIELSLGLSHQRSRNNTEAPPVAALALGNPV
                     VPTERTINNTRFNSAWAVLPVPTYRGSLVFGVSGVRAKNFDRAFERRTLDSVSAADFR
                     RTFVSERESGGLYAWSVGGAVDMSPNVSLGATLILWTGTDAFASSGSLDSAAGGPPGT
                     FRFADNIEDRYTGFTARVGALIQPNRMLTLGFTVDSPSWFSIDEDKIFRQTDTTGQLV
                     ESPQPLFQYDLIHPFAFGAGAKVNIAGLTLAGDLGFTDWSQMEYRNTPDARLQSDSLQ
                     QNYRDVLRINLGAEYTIPVTGVSLRAGFLSDPLPFKSSVIQTNRKFFTFGAGFLVDQV
                     LTLDAAVVLGDFGFNNYGNFFYTEKYNLTRVFVTAAWRL"
     gene            complement(3345..3845)
                     /locus_tag="AC16GM_11170"
     CDS             complement(3345..3845)
                     /locus_tag="AC16GM_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924268.1"
                     /translation="MKRNWALFIAAAVGTGILGTGCYTILAHPEVDQEKRYSTRDYYE
                     NCSSCHQNYHGTYDYGYYPDFYYDNWRWNRYYLDPWWYNWYGYDYNAPPGQTLPAEPG
                     QKGERRRDLRGRSPGRQRIESGQSSESQRGESGKEEKVKEEKTKKDEKKKEEEKPEKG
                     ERRRVP"
     gene            complement(3871..4692)
                     /locus_tag="AC16GM_11175"
     CDS             complement(3871..4692)
                     /locus_tag="AC16GM_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924269.1"
                     /translation="MARRRMAVQWNCGIAGFLIVFGLAAEAKAQQKREPQPKRPLVYF
                     VAPRRIIDLPTAGTLPRGYFDMELRMFANEGVLTAVNIGLTQHFELGLSYGAEHALGN
                     QGVNWNPRAEFNVKLRPISETMLLPAIAVGFESQGFGGWDAEQGQKRYAVKSRGFYGV
                     VSKSYVFTGFATGFHGGVNYSFENDDGDRSPNLFGAMDMRFSDNVAFLGEYDLALNDN
                     NGDRYGKGRGYLNLGVRWVFSDQLDMEVDLKDLFQNRRGTRSFGREFRLLYIEHF"
     gene            complement(4988..>5185)
                     /locus_tag="AC16GM_11180"
     CDS             complement(4988..>5185)
                     /locus_tag="AC16GM_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924270.1"
                     /translation="KPIELISIKKAEFSFIECFEWDRYGYASYPYYLVRIQKLPKYPH
                     FVGREALIETQDARVFYVDEK"
BASE COUNT         1085 a         1526 c         1439 g         1135 t
ORIGIN      
        1 gcggggttgt accgttcccg gtgctgaaaa acccgcagga aggtctcctg caccacgtct
       61 gaggcttctt cccggtcggc cagaagccgc accaccacgt tgaacaagcg gtctttgtag
      121 cgctccacca gattccggaa cgccgtctgg tcaccttttt ggaccgcccg gaacaactcc
      181 cggtcgccct ctcccctgtc ccgcccttgg cgaaattcct gcgacagatc catccgcttc
      241 atctcgcgcg cgtcgttcat ttccttctac acccaaagga cgaaatggtt cccgtttgaa
      301 atacgtgaaa aggggggcaa agattcagga gacgatagcc agacgaacct ttgacaacct
      361 ggagatgggg tggaactacc ccagcggtac tgacgtttca acgagggaaa aacggagttt
      421 gactcctggt agtcggggtc gtccctgacc ccgactgtcc ggctcagaga cgagccggac
      481 taccggtaag aggcaatgcg cattgcccgt ttcccagtgg tcaaaaaaaa atcaccctgt
      541 caaaggcaga gtgatcttca ttttgtggta aagagttttc gctcgcaggg tgcgggaaac
      601 cggctgccga aatcccgtag taggggggct cagcccaccg gctgcatctg tccgggctgc
      661 attggtccct ggtaggtgga acccgcttgc atcggccggg gcgccgcggg cgtcctttgg
      721 cccaccgtat cacggttgaa ctgatcgctt atccggcgca gtttcttctg cagatcttcc
      781 acccgctcgt tcatacggac cactgcccag gtcagagcca gaaccgacat tgaaatggca
      841 attatggtca ggtcgcccat agcttctcct tcggcgcaat gttcgcgtgc ctgtagggtg
      901 tatcgactta tccggcagat tatttagccc cccaccttcc cgcagacatt gattgtgcct
      961 ttccggtttt cgcggggctg ccgttcgacc tggagggaca aagccgtcgt tgacttccgc
     1021 ccgcggggct cttttgttat ccagactgat gtataaagcc agcaaacgag cctgcgatct
     1081 ctttttcgca actgtcgctt tggttctcct ttcccctttc tttgtgctcg ccgcgctggc
     1141 cattctcttc gattccggga gacccgtttt tttcaaacag acccgggtgg gcaaagacgg
     1201 ggtccttttt gaaatgctca agtaccgttc gatgatcaaa ggcgccgaac agtccgggcc
     1261 ccgggtgacc gccccgcgtg acccgcggat tacccgcatc ggcctcatct tacggaaaac
     1321 caaaattgac gaactgccgc aactggtgaa cgtgctgaaa ggggaaatga gtttggtcgg
     1381 cccccggccg gaagtgcccc gcctggcgag ccgttattca gccgaacaga agaaaatatt
     1441 tcaggtcaaa cccggattga cttcacccgg caccctctat tactaccgct ttcaagcgca
     1501 cgaccacccg gagggagttg acctggaaga ctactacctc cagaaacagt tgccggagaa
     1561 actggcctac gattttgcgt acttgaatcg cccagctatc ggtaaggatt tacttctgtt
     1621 gctcaaaacg ctctgggtta tcgtgacaac gccttttgtc cggccacaag cggttgaatc
     1681 gaaagggaat attatgccgg actaaagtct cggtacgagt cgttccgacc ggcctacgcg
     1741 tactttgggt aattctgacg acgctcttcg cccaaaagcg aaccccacag ccataggatt
     1801 ttgccttaaa gtcatgccga actaaagttc ggcctcgcgg gtggttgtgg tgcggctcgg
     1861 cggttttccc caaatccgaa atcccaattc ccaaatccgt acttacagcc gccaagccgc
     1921 ggtcacaaat acacgggtaa gattgtattt ctcagtgtag aaaaaattcc cgtagttgtt
     1981 gaagccaaag tcgcccagaa ccactgccgc atcgagcgtc agcacctgat cgacgaggaa
     2041 tccggcgccg aaggtaaaga acttccggtt ggtctggatc acgctcgatt tgaacggaag
     2101 cggatcggac aggaacccgg cccgcaggga gacgccggtt accggaatgg tgtactccgc
     2161 gccgaggtta atccgcaaca cgtcgcggta attttgctgg agcgaatcac tctgcaggcg
     2221 ggcatcgggc gtattcctgt actccatctg cgaccaatcg gtgaaaccca gatcgcccgc
     2281 cagagtgagg ccggctatat tgactttggc gccggcgccg aaagcgaacg ggtggatgag
     2341 atcatactgg aagagcggct ggggtgattc gaccagctgc ccggtggtgt cggtctggcg
     2401 gaagattttg tcttcgtcaa tcgaaaacca ggagggcgaa tcgacggtaa agcccaatgt
     2461 gagcatccgg ttgggctgga tcagtgcgcc cactctggcg gtgaaaccgg tgtagcggtc
     2521 ttcgatgttg tcggcaaagc ggaacgtgcc gggaggaccg ccggcggctg agtcaaggga
     2581 gccggacgaa gcgaaggcat cggtgcccgt ccagaggatg agcgtagccc ccaaagagac
     2641 attgggcgac atatccactg ccccgccaac ggaccaggcg tagagcccgc ccgattcccg
     2701 ttcgctcacg aaagtgcgtc tgaagtctgc cgcggaaacg gaatcgaggg tccggcgctc
     2761 aaaggcccgg tcgaaattct tcgcccgcac tcccgaaacg ccgaagacca gactgccccg
     2821 gtaggtcggc actggcagta ccgcccaggc cgagttgaag cgggtgttgt tgatagtccg
     2881 ctcggtaggc acgacaggat ttcctaaagc taatgccgcc acaggggggg cctctgtgtt
     2941 attgcgtgac ctttggtggc tcaagcccag cgaaagttca attcggtgga tgcgggctaa
     3001 ggcggcggga ttgtaattcg tcgccgagcc ggaggtggcg ttggccaagg ccgcgccgcc
     3061 catcgccatc acccgggcgt caaacccgaa gaagttggcc gctgagggct cgacgaaaac
     3121 gcccaattgc tccggctggg tcaggcctga cgaggtcagg cctaaaaaga ccaatggagt
     3181 gatgagcagt ttcttcatag tgcacctttt tccaaaaatc atttttgctc cggggcgacc
     3241 gagggcggtc gccccacgcg aagtagtacc ttccccgcgt ggacggggcg ccctcgccgc
     3301 gctcagatac acttctgaat cggcttccgc cattgatacg tttctcacgg cacccggcgc
     3361 cgctctccct tttccggttt ctcttcctct tttttcttct cgtccttctt ggtcttttct
     3421 tccttcacct tttcttcctt ccccgattct ccccgctggg attcgctgga ttggccagac
     3481 tcaatgcgct ggcgtccggg tgaccggccc cgcaggtccc ggcggcgctc ccctttctgc
     3541 cccggctcgg cggggagggt ctggccgggc ggagcgttgt aatcgtaccc gtaccagttg
     3601 taccaccagg gatcgaggta gtagcggttc cagcgccagt tgtcgtagta gaaatcgggg
     3661 tagtagccgt agtcgtacgt gccgtggtag ttctgatggc aggaactgca attttcgtag
     3721 tagtcgcggg tcgaatagcg tttttcctga tccacttccg gatgcgccag aatggtatag
     3781 caccccgtac cgagaatgcc tgtccccacc gccgccgcta tgaaaagcgc ccagtttctc
     3841 ttcatcgcgt tcacctcact ttcactacgt ctaaaagtgc tctatgtaca agagccgaaa
     3901 ctcgcggccg aacgaccggg tcccgcgccg gttctggaac aggtctttta ggtcgacttc
     3961 catgtcgagt tgatcggaga agacccagcg gacgcccaga ttcaggtagc cgcgcccctt
     4021 gccgtagcgg tcgccgttgt tgtcgttgag cgccagatcg tattcgccca aaaacgccac
     4081 gttgtccgaa aagcgcatgt ccattgcgcc gaagagattg ggggagcggt cgccgtcgtc
     4141 gttttcgaaa ctgtagttga ccccgccgtg gaagccggtg gcaaagccgg tgaagacgta
     4201 acttttgctc accacgccgt agaaaccgcg cgacttgacg gcgtagcgct tctgcccctg
     4261 ttcagcatcc cagccgccga acccctgcga ctcgaagccc accgcaatgg ccggtaaaag
     4321 catcgtttcc gaaatcggcc gcaacttgac gttgaactcc gcccgcggat tccagttgac
     4381 accctggttg cccagcgcgt gttcggcgcc gtaggaaagc cccagttcaa agtgctgggt
     4441 taaaccgata ttgaccgccg tgagcactcc ctcgttggcg aacatccgca gttccatatc
     4501 gaagtatccc cggggcaagg tcccggccgt cgggaggtcg atgatccgcc tcggcgccac
     4561 gaagtagact aacggccgct tgggctgcgg ctcgcgcttc tgctgcgctt ttgcctcggc
     4621 tgccaacccg aacacaatca agaatcctgc aatcccacaa ttccactgca ccgccatccg
     4681 gcgtcgggcc attttccctc cttgtgccat ctgtaattta gaccaccaga ggggaggcgt
     4741 caatgaaaac tgcgaacctg acgtgaagaa tctgcaccat ccagcgacgt ggaaagcaaa
     4801 tgggatgcct tgaaccttta agcgaccttg caaggagaag tcgtttctaa tggcaatgaa
     4861 aatgctttaa aacagttcat ggaactactg cgatgcacat ttttctttcg gggaaaaggg
     4921 ctgagcgcgt cgggcgaccg cctcggttgc ccctacaaat ttcgccgctc gggagagcgg
     4981 ccctacatca cttttcatct acataaaata ctcgagcatc ctgagtttca ataagagctt
     5041 cccttccaac aaaatgggga tatttgggca gcttctgaat cctcacgaga tagtacgggt
     5101 agcttgcgta gccatatcga tcccattcga aacactcaat gaatgaaaat tcagctttct
     5161 ttatagaaat tagttcaatg ggctt
//
LOCUS       DBNGZD010000431         2935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1387602, whole genome shotgun sequence.
ACCESSION   DBNGZD010000431 DBNGZD010000000
VERSION     DBNGZD010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1387602"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..345
                     /locus_tag="AC16GM_11185"
     CDS             127..345
                     /locus_tag="AC16GM_11185"
                     /inference="COORDINATES: protein motif:HMM:NF027249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR2924271.1"
                     /translation="MSKKRFLIVIEKTNGNFSAHPPDLLGCVATGRTREEVEQNMSEA
                     IALHLQGLREDHLPIPEPQAAADYIDVQ"
     gene            406..819
                     /locus_tag="AC16GM_11190"
     CDS             406..819
                     /locus_tag="AC16GM_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2924272.1"
                     /translation="MPNQAGFFTKLDTVIVRVRDLEKARLWYEEKLGLKAAFIDETEK
                     LAVLGTGGTTRLTIYQLKPREKLVTPPLAFEGSAGGERVAGTYPILCAEDIKATYELL
                     KSRGVAVEPEIHSGGGGQWFAFADLDGNKMEVCRF"
     gene            880..1152
                     /locus_tag="AC16GM_11195"
     CDS             880..1152
                     /locus_tag="AC16GM_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924273.1"
                     /translation="MAEVTAAHAAAVAQATRASGAIVKVAPEEFQRLVGRTESPFVVT
                     AYGGWPSKRHQYLTNYRGLFLYCKSEKPLQLPTSAEVVQAKSIWVP"
     gene            1204..1980
                     /gene="lipB"
                     /locus_tag="AC16GM_11200"
     CDS             1204..1980
                     /gene="lipB"
                     /locus_tag="AC16GM_11200"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR2924274.1"
                     /translation="MAKAEFGAVREIGVSVHQGQLRVVDVGVVPYGQALDVQLRLVEA
                     RRLGEIPDTLLFVEHPPVVTLGRAAKATSLLTPKDHLAKQGVELFEITRGGDVTFHGP
                     GQVVGYPIIDLNARFDSVHRKRGRDLHQYLRDLEEVLLQTLAAFGLKGERQAGFTGAW
                     VNNRKIVAIGVAVKHWISYHGFAFNVNVDLNYFNLIVPCGLAGKEVTSLERELGRPVS
                     MEETKANLSKAFAQVFEKEIVSKAGEELAERVKSPSLLTA"
     gene            2152..>2935
                     /locus_tag="AC16GM_11205"
     CDS             2152..>2935
                     /locus_tag="AC16GM_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HIR2924275.1"
                     /translation="MRKYLEKVQNFRLLPVFVAVSMAIGITIGKIYGISNFQLTPPID
                     AVKSIFQGTYQFSLSNSLALGVVLGLFLMIYPAMTKVKIEDLGQAARSPKQLLIVLFF
                     NFAIAPFLMLFFARLFLSGSQDLYVGLVLYGLAPCIAMVIVFTFLALGNNALAIVLVA
                     INSVAQMILIPVYAKLLLGNITFDVFVVGGSVLLYLGLPLLAGFFTRRIAVRKLGEDG
                     FKSFQTYLNSLSIVGLLFTVIVMFALKGDLILQKPLVILQMAI"
BASE COUNT          686 a          762 c          855 g          632 t
ORIGIN      
        1 agctggaaac acttagagct gaaatcgcta atcaggttag acaagtgctt agtaaccccg
       61 atgccgtaga taatccccta agtgttttct ccccaaatct gaaggctacc ttctaaagaa
      121 gactcagtga gtaagaagcg ttttctcatt gttatcgaaa aaacaaatgg gaatttctcc
      181 gctcaccctc cagaccttct tgggtgcgtt gccactggca ggacgcggga agaggtggag
      241 cagaacatgt ccgaagcaat tgctctgcac ctgcaaggtc tacgcgaaga tcacctgccg
      301 ataccggagc cgcaggccgc agctgactac attgatgttc agtgaatctt accgaacaaa
      361 ctgccgataa ttttcaaatt ttgatctctc tgcgcggggt gatatttgcc aaatcaagcg
      421 ggatttttta ccaaactcga tacggtgatc gtacgggtgc gcgatctcga gaaggcccgg
      481 ctctggtacg aggaaaagtt ggggctcaag gccgccttca tcgatgagac tgaaaagtta
      541 gcggttctgg gcactggcgg cacaacacgt ttgaccatct accagttgaa gccgagggag
      601 aagttagtca caccgcccct ggcgttcgaa ggttcagccg gaggagagcg ggtggcgggg
      661 acgtacccga ttttgtgcgc cgaagacatc aaagcgacgt atgaactcct gaagagccgg
      721 ggagtggcgg tggaaccgga aatccactcc ggcggaggcg ggcagtggtt tgcgttcgcc
      781 gatttggacg gcaataagat ggaagtgtgc cgcttctgag agcagaacac agaatacagc
      841 atacaggaca cggggaaaga cacaatgggc ggaggagcgg tggcggaagt aacagcagca
      901 cacgcagcgg cggtggcgca ggcaacgcgg gcgtcgggag caattgtaaa agtggcgccg
      961 gaagaattcc agcggttagt cggtcgcacg gagagtcctt ttgtcgtcac ggcttacggc
     1021 gggtggccgt cgaaaaggca ccagtacctg accaactacc gggggctctt cctctactgc
     1081 aagagcgaaa agccgttgca attgcccacg agcgcggaag tggtgcaggc gaaaagcatc
     1141 tgggtgccgt aggaaggcgg aacacggaat acgggagaca gaacacaggg aaagcaaaca
     1201 cgaatggcca aagccgagtt tggagcagtg cgagaaatcg gagtctccgt tcaccagggt
     1261 cagttgcggg tcgtggacgt gggcgtggta ccctacggac aggcactgga tgtgcagttg
     1321 cggctggtgg aagcgcggcg gctgggagaa attcccgata ccctcctctt cgtggagcat
     1381 ccgccggtcg tcactctggg gcgggcggcg aaggccacga gtcttcttac tcctaaagac
     1441 catctggcaa agcagggagt cgaacttttt gaaatcaccc gcggtggaga cgtcaccttc
     1501 cacgggccgg ggcaggtggt gggctacccg atcatcgacc tcaatgcgcg gttcgactcc
     1561 gttcaccgca agcggggaag agatttacac cagtatctgc gggacctcga agaggtgctc
     1621 ctgcagacgc tcgccgcctt cggactgaaa ggggaacggc aggccgggtt caccggagcg
     1681 tgggtcaaca accggaagat tgtcgccatc ggcgtggccg tcaaacattg gatctcctac
     1741 cacgggtttg cgttcaatgt gaacgtcgat ctcaactact tcaatctcat cgtcccctgc
     1801 gggctggccg ggaaggaagt aacgtccctg gagcgggaat tggggcgacc ggtatctatg
     1861 gaagaaacga aggcaaactt aagcaaggcg tttgcgcagg tattcgagaa agagattgtg
     1921 tccaaagcgg gagaggagtt agcggagcgg gtcaagtcac cctccctgct gacagcctaa
     1981 gcgcggcggg ggcgccgcgc ccacgcgagt tttttttcac cacggagaca cggtcggaga
     2041 ctcgactctt cgagaggaca cggagaaaag gaaatttcaa ggattctccg gaaaagggcc
     2101 gggtcgctca ccggcaccag ttcatattct cattcttagg cagcaattgc aatgaggaaa
     2161 tacttagaaa aggttcagaa cttcaggctc ctgccggtat tcgttgccgt cagcatggcc
     2221 atcggaatta ccatcggcaa aatatacgga atttcaaact ttcaactgac gccgccgatc
     2281 gatgccgtca aatcgatatt tcagggaacc taccagttca gtctttccaa cagcctggcc
     2341 ctgggggttg tgctcggact tttcctcatg atctacccgg ccatgaccaa agtcaaaata
     2401 gaagatctgg gccaggccgc ccgctctccc aaacaactgc tcatcgtatt atttttcaat
     2461 tttgccatcg ctcctttcct gatgctgttt ttcgccaggt tgttcctttc cgggagtcaa
     2521 gacctctacg tcggcctggt gctctacgga ctggccccgt gcattgcgat ggtcatcgtg
     2581 ttcaccttct tggctttggg gaacaacgca ctggccatcg tcctcgtggc cataaattct
     2641 gtcgcgcaga tgatactcat tccggtttac gcaaaacttt tactggggaa tatcaccttt
     2701 gacgttttcg tcgtgggcgg gagtgttctt ttgtatctcg gcctgcctct tttggcaggc
     2761 tttttcacgc gccggatcgc agtcaggaaa ctgggtgaag acggcttcaa gagcttccag
     2821 acctacctca attctctttc catcgtgggc ctgctattca ctgtaatcgt catgttcgcc
     2881 ctgaaagggg acctcattct gcaaaaaccg ctggtcattc tccagatggc catac
//
LOCUS       DBNGZD010000432         5808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_971157, whole genome shotgun sequence.
ACCESSION   DBNGZD010000432 DBNGZD010000000
VERSION     DBNGZD010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_971157"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC16GM_11210"
     CDS             <1..719
                     /locus_tag="AC16GM_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR2924276.1"
                     /translation="LQKTDLLVKVGLDLEIGWLPVLLDGARNPKIMPGAPGHVDASKS
                     IAVLEVPTGKVERSQGDIHALGNPHYWLDPENAKASAASIADGLKAIDPSDAAFFEAN
                     LKTFNETLTQQEARWVRLMEPFQGAKVITYHNSWPYFVRRFGLNVVGYVEPKPGIPPP
                     PSHVAELIDQVKREGIKLIIMEPYFSEKIPQLVASQTGCRVLVLPPSVGGVKGVDDYA
                     ALLDYDLNQIVSALKGQGTP"
     gene            725..1561
                     /locus_tag="AC16GM_11215"
     CDS             725..1561
                     /locus_tag="AC16GM_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR2924277.1"
                     /translation="MLKLPFMLQALAGCLLLAVTLSYFGIHVVLRRIVFVDLALAQVS
                     SIGVAVAMLVEGNVTLYALAFTLLGAAVFSIRIKERRIPQEAAIGFVYAVASAVAILL
                     ISKSPHGEADVLKILFGNILAVNKKQLLEMAFVFGGVGILHFLLHKKIFAISQAEAHH
                     EFSARHLLLWDLLFYLTLGLVIAFAIRSAGVLLVFSFLIAPGIFAIGLGARSLGALYA
                     LAAGLGVINSFTGLYLSYRYDLPTGAALIATFGAFIVLAGAVRLVFLRRNKTENEVQP
                     EG"
     gene            complement(1587..2309)
                     /locus_tag="AC16GM_11220"
     CDS             complement(1587..2309)
                     /locus_tag="AC16GM_11220"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR2924278.1"
                     /translation="MHDVIYFVSDLHLGSREDHAGGATQKKFLNFLGHIEKDASALYI
                     VGDLFDFWFEYKHVIPKVSLEVLLALRQLTGKGIALTLFFGNHDPWQIDYLQTQLPAA
                     VIPWDMTVEAQGKKIYLCHGDGLAPSDRGYRLLKKILRNKINVALYRLLPCDWAIPLA
                     RRVAGASRNYTTERGKNFLPEYEAFAQKKLNEGYDAVILGHAHEPVEKKWGGKAYLNL
                     GDWLTHFTYAELRHGTLRLKRF"
     gene            2513..3787
                     /gene="murA"
                     /locus_tag="AC16GM_11225"
     CDS             2513..3787
                     /gene="murA"
                     /locus_tag="AC16GM_11225"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR2924279.1"
                     /translation="MDKFVITGRQPLSGSVKIEGSKNAVLPILAATLLVEKGTTVIHN
                     VPDLVDIDTFLKVIAQLGAKVNHDRQAKTVSIEASTLTKYEAPYDLVRKMRASFLVLG
                     PLIARLGKARVSLPGGCVIGPRPVNLHVYGFQRLGAQLSEDRGYIVAEVPPATGQLVG
                     TTICFDRPTHTGTENLMIAACLAKGKTTLVNAACDPEVVDVANYLNATGGKVEGAGTS
                     TVTITGVRRLHPVEYTPMPDRLESATFLMAAAVTGGYVEIDNGKPEHLDMVVSKLTEM
                     GADIHLAGSRLKVKGPNRLKAVSVTTYPFPGFPTDLQPQLMALCAVANGTSHVRETVF
                     EDRFTHVMEMLRLGADIKVARDEATIHGVAKLKGASVMASDIRAGAGLVLAGLAAEGE
                     TEVLRVYHIDRGYNSFEEKLSHLGASIKRVAA"
     gene            3855..4172
                     /locus_tag="AC16GM_11230"
     CDS             3855..4172
                     /locus_tag="AC16GM_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924280.1"
                     /translation="MNAKKITALVALVFYLPGILLCLCCDFGSAEATRAHFSVCPNVP
                     GNPVPESTCEDCGTCCGQQVLIPSFSQTGVLDESPTNKLITSSFLLSEQLHSLSIYHP
                     PEA"
     gene            4239..5465
                     /locus_tag="AC16GM_11235"
     CDS             4239..5465
                     /locus_tag="AC16GM_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2924281.1"
                     /translation="MLKVFWAFTVFVFVSVAQAQEKATYSLEQAVALALRQNPEILAA
                     ERQIDAANGRRSQASAPDRPELSFKWEKIPSGGSFGQAGDRIIEIGQNLPFPPKLFLK
                     KSAAGRDVDIAFENLGRIQTLVSARVKKTYYTVFFQKKQLESLEFTAGLLRLFQEATL
                     SKYQAGELAYFEVVRAKVEAAKTENEIIGAKAELASAKTDFNLVLGRNSSEDFELTDK
                     AVFAPYDKSKAETVRDLLQKSRTLKIAELSKERENQNLRLARWGLVPDLKLEGGVQSE
                     AGRDFRPNIGFGLSIPLWWWGPKGGITESRAAFQVSQIREAALNRVLAAEIERAHTLV
                     KSAENQVSLFEKSLLREVDEELKAGINAYQYNQIDALSLLDIYRTVKTTKIEYYKAFF
                     NYLSALADLEAAGEEK"
     gene            5462..>5808
                     /locus_tag="AC16GM_11240"
     CDS             5462..>5808
                     /locus_tag="AC16GM_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924282.1"
                     /translation="MKGRVLCGSLERKSVFMLLGFWLALGLLGCGEKGEREEREREAG
                     KSEGAKAVPGLVTLTSEGIKLAGIKVERVSLRNVPLQFSAAGTVGFNEKKLVHITSRV
                     SGWVEKIHAFAGDR"
BASE COUNT         1259 a         1542 c         1731 g         1276 t
ORIGIN      
        1 aactccagaa gaccgacctc ttggtgaaag tggggctcga tctcgaaatc ggctggctgc
       61 cggtgctttt ggacggcgcg cgcaatccga aaatcatgcc cggagcgccg gggcacgtgg
      121 atgcgtctaa gtccattgcc gtgctcgaag tgcccacggg caaagtggaa cgttcgcagg
      181 gcgacatcca cgctctgggc aatccgcact actggctcga tccggaaaac gccaaagcga
      241 gcgcggccag cattgcggac gggttaaagg cgattgatcc ctctgatgcc gctttttttg
      301 aggccaacct caaaacgttc aacgaaacgc tgacgcagca agaagcgcgc tgggtaaggc
      361 tgatggagcc gttccagggc gccaaagtga tcacctacca taattcctgg ccctactttg
      421 tgcggcgttt cggcctgaac gtggtggggt acgtcgagcc caaaccgggc attccgccgc
      481 cgccatcgca cgtggcggag ttaatcgacc aggtgaaacg cgagggaatc aaactgatca
      541 tcatggagcc gtatttctcc gagaaaattc cacagttggt ggcgagccag accggctgcc
      601 gggtgctcgt cctgccgcct tcggtgggcg gggtgaaagg agtggatgac tacgcagcgc
      661 tcctggacta cgacctcaac cagatcgtga gcgcgctgaa agggcagggg acgccatgat
      721 tgagatgctg aaactcccct tcatgctcca ggcgttggcc ggctgcctgc ttctggcggt
      781 cacgctctcc tacttcggca tccatgtcgt cttgcggcgg atcgtcttcg tcgatttagc
      841 gctggcgcag gtttcctcta tcggcgtggc ggtggcgatg ctggtggagg gcaacgtcac
      901 cctctacgcg ttggccttta cccttctggg cgcggcggtg ttctcaatcc ggatcaagga
      961 gcggcggatt ccgcaggagg cggcgatcgg tttcgtttac gcggtggcct ccgccgtggc
     1021 cattcttctg atttccaaat cgccgcacgg cgaggccgac gtgctcaaaa tcctcttcgg
     1081 gaacattctg gcggtgaaca aaaagcaact gctggaaatg gctttcgtct tcggcggcgt
     1141 ggggattttg cacttcctgc tccacaaaaa gattttcgcg atttcgcagg cggaggcgca
     1201 ccacgaattt tccgcccggc acctcttgct ctgggatttg ctcttctacc tcaccctggg
     1261 gctcgtgatc gcctttgcca tccggagcgc gggggtgctt ctggtttttt cgttcctcat
     1321 tgcgccgggg atttttgcca tcggcctcgg agcgcggtct ttgggagcgc tctacgcact
     1381 ggcggcgggc ctcggcgtca taaacagttt caccgggctt tacctttcgt accggtacga
     1441 tctgccgacc ggggcggcgc tcattgccac cttcggggcc ttcatcgttc ttgccggagc
     1501 ggtacgactc gtatttctcc ggcggaataa aaccgaaaac gaggtgcagc cggagggata
     1561 gttttagcga gtcaacctaa gaaagttcag aaccgcttca gtcgaagcgt gccgtggcgc
     1621 agttcggcgt aggtgaagtg ggtaagccag tcgccgaggt tcaaataggc tttgccgccc
     1681 catttttttt ccaccggttc gtgggcgtgg cccaggatga ccgcgtcata cccttcgttc
     1741 aatttcttct gcgcgaaggc ctcgtactcc ggcagaaaat tttttccgcg ctcggtggtg
     1801 tagttgcgtg acgcgccggc tacccgcctg gccaaaggaa tagcccaatc gcaggggagg
     1861 agacggtaga gggcgacatt gatcttattt cgcaggattt tcttcaacaa ccggtaaccg
     1921 cggtcgctgg gagccaaacc atcaccgtgg cagagataga ttttctttcc ctgggcttcg
     1981 acggtcatat cccaggggat gacggcggca gggagttgcg tctgcagata atcaatctgc
     2041 caggggtcgt ggttgccgaa gaacagggtg agggcaatgc ctttcccggt caactgccgc
     2101 agcgccagga gcacttccag cgacaccttc gggatgacat gtttgtattc gaaccagaaa
     2161 tcgaataaat ctccaacgat gtagagcgcg gaggcatctt tttcaatatg cccgaggaag
     2221 ttcaggaatt ttttttgggt cgctccgccc gcgtgatctt cccgcgagcc caaatggagg
     2281 tcggatacaa agtaaatgac atcgtgcatc ggtcgtaatg tagaataggg cagagccgtt
     2341 ttgcaagtga acactgtccg cttcggatgg ggctataaaa tcgtggatgc cgtatgccga
     2401 aagaataaag gagagacggg tatggagcac cgcgccttga caagcgaaca tccagaggta
     2461 aattcgtttt gcaattgcac cgcaaacgtg aacaggcagg ggaatcgcac tgatggataa
     2521 atttgtcatt acgggccgcc agccgctttc gggctccgtg aaaatcgaag gttccaagaa
     2581 cgccgtgttg ccaattttgg ccgccacgct tttagtcgag aaagggacca cggtcatcca
     2641 caacgtcccc gatctggtgg atatcgacac ctttctgaaa gtgattgcgc agttaggcgc
     2701 gaaggtgaac cacgatcgcc aggccaaaac ggtttcgatc gaggcgagta cgctcaccaa
     2761 gtacgaagca ccgtacgatc tggtgcgcaa gatgcgcgcc tcatttctgg ttttgggacc
     2821 tttgattgcc cgcttgggga aagcgcgcgt atctttgccc ggtggctgcg tcattggccc
     2881 gcggccggtg aacctgcacg tctacgggtt tcagcgtctg ggtgcccagc tttccgaaga
     2941 ccgggggtac atcgtggccg aagtgccgcc ggcgacgggg cagttagtcg gcacaacgat
     3001 ttgctttgat cgtcccaccc acaccggcac cgagaatttg atgatcgccg cctgtctggc
     3061 caaggggaag acgaccttag tcaacgcggc ctgcgatccg gaggtggtcg atgtcgccaa
     3121 ttacctgaat gccaccgggg gaaaggtgga aggcgccggc acctccacgg tcaccatcac
     3181 cggcgtgcgc cgcctgcacc cggtggagta cacgccgatg ccggaccggt tggaatcggc
     3241 cacgttcctc atggccgcgg cggtgaccgg ggggtacgtc gaaatcgaca acggcaagcc
     3301 ggagcactta gacatggtgg tgtccaaact gaccgaaatg ggggccgaca ttcatctggc
     3361 cggcagccgg ctgaaggtga aagggccgaa ccggctgaag gcggtgtcgg tgaccaccta
     3421 cccctttccc ggtttcccca ccgatctgca gccccagctg atggcgctct gtgcggtggc
     3481 caacggtacg agccacgtcc gcgaaacggt ctttgaagac cgcttcaccc acgtgatgga
     3541 aatgctgcgc ctgggcgccg acatcaaagt ggcccgggac gaagcgacga ttcacggcgt
     3601 cgcgaagttg aaaggcgcgt cggtgatggc ttcggacatc cgggccggcg ccggactggt
     3661 actggccggt ctggctgcgg aaggggaaac cgaagtgctg cgggtctatc atatcgaccg
     3721 ggggtacaac agttttgaag aaaaactgtc ccacctcgga gcgtcgatca agcgggtggc
     3781 ggcgtaggga gccctgtcgc tgcgattgat gcagaaattc ttgacatccc ggaaatagaa
     3841 tttatattga cgttgtgaat gccaagaaga taacagctct tgtagcttta gtcttctatt
     3901 tgcctggtat tttgctgtgc ctgtgctgcg acttcggttc tgccgaagca actcgtgccc
     3961 atttttcagt gtgtccaaat gttccaggta atccagttcc agaaagtacg tgcgaagatt
     4021 gtggaacctg ttgcggccag caggttctta tccccagttt ttcgcaaaca ggtgttctcg
     4081 acgaatcccc tacgaacaag ctcatcacca gtagtttcct gctctccgag cagcttcact
     4141 ctctttcaat ttatcaccct ccggaagctt agtgagtctc ctttttagtc ccccaagttg
     4201 gggtcatttc agtcaaatct tccaatcctg gaggcatgat gttgaaagtt ttttgggctt
     4261 ttactgtttt cgtctttgta tcggttgctc aggcacaaga gaaagcaacc tactccctgg
     4321 aacaagccgt ggctctggcg ttacggcaaa atccggaaat tctggctgcc gagcggcaaa
     4381 tcgacgcagc caacggtcgg aggtcccagg cctctgcccc ggaccgacca gaactctctt
     4441 tcaagtggga aaaaattcct tccggcggct ctttcggcca ggccggcgac cggattatcg
     4501 agataggaca gaacttgccg tttccgccca agctgttttt gaaaaaaagc gccgcgggcc
     4561 gggacgtgga catcgctttc gaaaacttag gccgcatcca gaccttagtt tctgcgcggg
     4621 ttaagaagac ctactacacg gttttcttcc agaaaaaaca gctggagtcg ctggaattta
     4681 ccgccggact tttaaggctg tttcaggaag cgaccctgtc gaaatatcag gcgggtgaac
     4741 tggcctattt tgaggttgtc cgcgccaagg ttgaagcggc caaaacggag aatgaaataa
     4801 tcggggcaaa agccgaactg gcctcggcca aaactgattt caatctggta ctgggaagga
     4861 atagctcgga agattttgag ttgacggata aggcggtttt tgccccctat gataagagca
     4921 aggcagaaac tgttcgcgac cttctgcaaa agagccggac cctgaaaatc gcagagcttt
     4981 caaaagaaag agaaaaccag aatctgcggc tggcccgctg gggcctggtg cccgacctga
     5041 agctggaagg gggggttcaa agcgaagcag gtcgggactt tcgccccaac atcggattcg
     5101 gcctgtctat cccgctctgg tggtgggggc ctaagggagg aattaccgaa agccgggccg
     5161 cctttcaagt ttcccaaatt cgggaggcag ctttaaaccg agtattggcg gcagaaatcg
     5221 agcgggccca taccttggtg aaatcggcag aaaaccaggt ttctcttttc gagaaaagtc
     5281 ttttgcggga agtggatgag gaactaaagg ccggcatcaa cgcctaccag tacaaccaga
     5341 ttgacgcgtt aagtcttctg gacatttatc gaacggtcaa aacgaccaaa attgaatact
     5401 acaaggcctt cttcaactac ctttcggctt tggccgattt ggaagcggcc ggggaggaaa
     5461 aatgaaaggc agagttttgt gcggcagtct cgaacggaaa agcgttttta tgttgcttgg
     5521 attctggctg gctttaggat tattgggctg cggggaaaag ggggagcggg aagaaagaga
     5581 aagggaggcg ggcaaaagcg aaggggcgaa ggccgttccg ggtttggtaa cattgacttc
     5641 ggaaggaatc aaactggccg gaatcaaagt cgaaagggta tctctgcgca acgtccccct
     5701 gcagttttcc gccgccggaa cggttggctt taacgaaaaa aagctggtgc acataacctc
     5761 gcgggtatcc ggctgggtgg agaaaattca cgcctttgcc ggtgaccg
//
LOCUS       DBNGZD010000433        12550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_901833, whole genome shotgun sequence.
ACCESSION   DBNGZD010000433 DBNGZD010000000
VERSION     DBNGZD010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12550
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_901833"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="AC16GM_11245"
     CDS             complement(<1..1536)
                     /locus_tag="AC16GM_11245"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2924283.1"
                     /translation="MDTQPAVRQGRRRRVAAGRILLFSFTLLIGWAVCSGTVQAQQST
                     VDTLRILDAFGLPGDTAVIVQIYVRNDSFPVAGVTFRLIFDTTFLTPVFLPPTQTVPF
                     PVRFETVNRGTALPEGGVALDTSATGVVNGLLFAGFSGIPKIERGAGTIMHVFFNIKP
                     GVSLGATSPLRLINDTAQGGLLNALSDTLGNLILPVLANGTLHIQQPPRINRAPVIQP
                     IAAQQVTQGQRLTFTVTAIDADGDPLTLQATALPANATFATVTGDSIASGTFTFVPSF
                     SQEGDVFPIFRATDSVGAITTLSVKITVKKLLRDVLSTISDTSAGKQPVGALAGRGGV
                     LFPIDLANTKDVLGVQFDFNFNPTILGVDSVVPTGRTQGFDRLLFDTIAPGVVRVVAY
                     GLNLQRLQPGVTPTILNVALSVNPAAVTGKYPVTFTNAFISFDPNLPSESLAVENGIF
                     SVDLLGDVNLDARVDVGDAVNCVAFILGSLDTTFRRFDAADVNRDSTLNVLDLAGIEN
                     IIFG"
     gene            complement(1537..3255)
                     /locus_tag="AC16GM_11250"
     CDS             complement(1537..3255)
                     /locus_tag="AC16GM_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2924284.1"
                     /translation="MTGKIRSGRVSVLVAAVVLGCAVGAPAGEQFTNRPPRLGVPDSI
                     HVFMGDSTRFTVTANDPNGDPVTVTVLRLPPGASFTGNNPWLFRWWTAARDTGRYWVV
                     FWAKDPHGGGDVESVLVAVQTATLFWLQVLTASGYNDGDVVVPVVLGNQDSVGGMDLL
                     INYDISALTLSSVTRTGSRLADWELFSVVPNAGGNAGDIRIVGIANTNAPGVTPPLAP
                     GIGPVAFLSFHITRDVNLAGFAIPVTFAFRAPSDNVLSDPRGNLIAQSAITFQDGAVL
                     ILTPGTPLVGDINLNGAAFDIGDAIRLINFFLNPGAFPLNAQQRVNSDINGDGIPATV
                     GDLVALIRRILEGGGGQTGGFAKNAVAAAAQVTVQPLVRNDSLILSLAAPVPVSGLFL
                     EFGNVPATVGQPELRLADPDFTLASQNVGSALRLVLYSSQGRRLPAGCTEVLSIPLRG
                     ARAPEISQAACCDSSGDLLETAVGRAVTSVTPVDFFENYPNPFNPATTIAFRLPQGGR
                     VNLSIFNILGQKVKTLVDGTLSTGRHELKWDGTSDSGQPVASGVYFSRLITEQSEAIG
                     KMTLLR"
     gene            complement(3704..6574)
                     /locus_tag="AC16GM_11255"
     CDS             complement(3704..6574)
                     /locus_tag="AC16GM_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2924285.1"
                     /translation="MRRFSIFVIACSLLVWAGVAVAQEAGAGPFVDSLIIESKTFSNG
                     SIGFISVMVSNDSACNGISLPIVITTLSGGAFGKVDSIKKNANIDPLSATVSLAGVNG
                     TSPDSAFVLSVDPGGGSFPAGARREAYKYWVTNNSNLGQYQLDTNRLSASKTLKFTHP
                     PTQTSFPKVVKGIETIANLPPTCDGTAPISVHFGSPASQSFTGADPEGDPLTFVKISG
                     LGAVAANGDWTLGAGATVCGDVGVYQVCAAVSDPLHHNADTCCFTLTITQNPPVLACP
                     PNQSVLHGALIDVPIGASDDACPSPLNVFLASGPGGVTGSPPGPFRYRFQTSCEDVGQ
                     HVVTLKATDGKDTVQCSFNVTVTNQAPSITCPGGVIQIQANSCDNETFPTTDADGDAL
                     TYSLGNSVPPSVGTITPGGAYQYCPTPAEAGPQHAWIVATDACGAKDSCEKNFLVVVG
                     QKFCLDIEHVTAFLGTHTVPVCVNEPLNGSDPDIPNGSCVGGFNFLFAYDPTCLSFQK
                     ATMGSFLIRCQWEFFTFRFGPMGNCGNGCPSGFIRVIALADINDAAQHHPPRGACQTD
                     NAGQWLCLWFEVTRDRNFQNTCCPIRWWFWDCNDNTISDCSGNQLWTVDSVYNAEGVG
                     LDLATEFGLGVDVSNCVSSGGPNKPRSKKFICLHDGKICIPADSTIDARGDLNLNGLA
                     NEIADAVLYENYFLQGSSVFSSDPQRRQAQIAASNVNNDAFALTVADLVYLIRILTGD
                     AQPFPKVVPGDVKVEFATTRTADGYSIQGNAGVDLGGALMVFKTTGTVSDVRTVGTAS
                     DMNVKFNQEGGQLRVLIYSHERGMKIDAGQSPVLNIVTDGNLTLVDGDASSYDGYPVA
                     ISGLGKPVPSGFAVSQNVPNPFNPSTRITLSLPAASNYTLTIYNVAGQVVKSFTGHGD
                     AGDVTITWDGTDNTGAKVASGIYFYKGVAGNSSDTKKMVLMK"
     gene            complement(6814..7671)
                     /locus_tag="AC16GM_11260"
     CDS             complement(6814..7671)
                     /locus_tag="AC16GM_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2924286.1"
                     /translation="MRVRSFLLTAGAVVGVSGMAQAQLTVQVQVVDTVLWPGDQTAKI
                     RIAMTNPNLKIGGYTLTLTIDNPQGVEFIFNRVDTLPPETAYVCVNPPACTLRDTVIN
                     RPVVYITNVDTNGSRTRNFSKVDGRWQSEIFLRVVAIAGSNSNAIPFGNGTLLRLPVR
                     VNPVADTFSQRAIQVGFALDPTQTFFSDTTGNILCFTGGPSCGLTKNAGTVTIPRSLR
                     GDVNFDGVIDIVDVVRLVQYVVFGTQEPIPDLANGDVRCPPDGVVDIIDIVSLIQYVV
                     FGVPEPCPL"
     gene            complement(8343..9119)
                     /locus_tag="AC16GM_11265"
     CDS             complement(8343..9119)
                     /locus_tag="AC16GM_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR2924287.1"
                     /translation="MENSSVTCPACQAANSAGSRFCNRCGRPLPENLRCRWCQTLNSR
                     EASVCSGCGVPLPVPQAQPGADAASTSSKKQKTKPQQRGVRPRYPLIVFGLLGAGAVI
                     FLFIYLRSTRPPIPSKAGQSGEEAALAEINRLLSANDRQTAYRELVLQGNQAMDSQRF
                     PEAILYYTHALAIDSSAVDVRVDLGACYHFVGENQKAKGEFLRALNASPQHLVALFNL
                     GVVYHGLGQKDSARVVWQKYLRLAPNGPLVPQIQNLLSQQ"
     gene            9307..11670
                     /locus_tag="AC16GM_11270"
     CDS             9307..11670
                     /locus_tag="AC16GM_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR2924288.1"
                     /translation="MLLRQRLLPVLLFAVSVWVVTPAKAGAVDHHITMLAHVVDDTMR
                     GCWGYIDSLGREIALVCSYSNLDLYDVTDPTNPILRKRVPQIADATDLKAVRPYHDSI
                     AYVGNQSGLMQIVYIWNADNAQTIGSFATPLTGGNHTIEIFGDYMYLGMNGASDRRIQ
                     IWSLANPAAPTKIISLANPGTGFNDGHDCYVHGDTIYTHHLTAGFKVWNIANKAAPVG
                     LATVSYPGVFQHASWTSSDGKILYTDDETDFGRLRVWNVSDLRQIIQLANYAPSANAG
                     GVVHNVYLFQGDDLAYTGCYEEGVRILDVSDPTYPIEVGGFDTSPGNNPPGPPSAPQE
                     ARDGVWDAYLFPSGTLIATDRKFGFWTLRFDGSRAGRIEGTVTNSVTGQPLPDAKVKL
                     INLTNLPNVPSVLTGTDGSYGIRTGSVLQNVEVSRFGFRTDTVQVTGIVADTVPLNVA
                     LDPVPYGTISGQVTRSATGDPIAGVIVGLAGEPYHSDTTDGSGNYSINFVPADSNFLI
                     ETWKWGFQSAQATVLVTAGGNATANFGLRSGYSDDFEHDLGWTVGDAGDDATAGFWTR
                     VEPFGMKNFANSTVQPDTDHTAPRGQRCWITGQGRFGIVANDQDVDDGKTTLLSPMMD
                     LSGFSTPVLSYWRWFSNNGDHNPDEDTLEVSISSDGGISWVVLERTTATENRWTSKQF
                     CLTSKINLTSNVRFRVVISDRRGPSLVEGAFDDFAVLDRAAGDINNDRTVDIADITQL
                     ITHVVFGTQPPNPLCFGDLNCDGVIDIVDVVQLILFVVFGAPAPCTP"
     gene            11844..>12550
                     /locus_tag="AC16GM_11275"
     CDS             11844..>12550
                     /locus_tag="AC16GM_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924289.1"
                     /translation="MRKLTLFTLLTLGAPGFFLCSKKPTNPTPPVVVEKPCADLDSTL
                     TFTCCRQWVRDPTWSPDGNTIAYADLEINWGLHLVNPDGSNDHLIVPAVGVFELDFSS
                     DGQLILFLWLRTNQPPGSTGIYKIRTSGKDTTKISSTYDDFYPRWSPDDKLIAFERDI
                     SSGDPQNGVYLMNADGTGARQIFPLGQVPDWSPDGRLVVTKGANLYIIDTLGNVLLKL
                     TNFLADSLGRGIRAGSGR"
BASE COUNT         2643 a         3775 c         3592 g         2540 t
ORIGIN      
        1 gccgaagatg atgttctcaa taccggccag atcaagtaca ttcaatgtgc tgtctcggtt
       61 cacatcggca gcatcaaacc tccgaaacgt tgtgtctaag ctgccaagaa taaaggcgac
      121 gcaattcact gcgtcgccaa catccactcg ggcgtctaga ttaacgtcac ccaagagatc
      181 cacggaaaaa atgccgtttt ccaccgctaa cgactctgaa gggagattgg ggtcgaacga
      241 aatgaaagcg ttagtgaagg tcacggggta cttccctgtt actgccgccg gattaacact
      301 caacgcaaca ttgagaatcg tgggcgtcac tcccggctgg agtcgctgaa ggttgaggcc
      361 gtaagccaca actcgaacca ctcccggagc tatagtatcg aaaagcaagc gatcgaatcc
      421 ttgcgtgcgt ccggttggca ccactgaatc aactccgagg attgtcggat taaagttgaa
      481 gtcaaactgc acccccaaca catctttggt attagccagg tcaatgggaa agagcacacc
      541 accccggccc gccagcgccc ccaccggctg tttgcccgcc gacgtatctg aaatcgtcga
      601 gagaacatcc cggagcaatt tcttcacggt gatttttacg gagagggttg tgattgcgcc
      661 aaccgagtcc gtggcgcgga aaatcgggaa aacatcccct tcctggctga aactcggaac
      721 aaaagtgaac gtgccgctcg ctatgctgtc gcctgttacc gtcgcgaaag tggcattcgc
      781 cgggagcgcg gtggcctgca gggtcagtgg gtcgccgtcg gcgtcgatgg cggtaacggt
      841 aaaggtcagc cgttgtccct gcgtcacctg ctgcgccgca atgggctgaa tgaccggtgc
      901 ccggttgatg cgcggcggct gctggatgtg cagcgtgccg ttagccagaa caggaagaat
      961 gagattaccg agggtatccg acagcgcgtt taacagccct ccctgggcgg tgtcattaat
     1021 caaacgtagt ggactggttg cacccagact cactccaggt ttgatgttga aaaaaacgtg
     1081 catgatagtt cccgcccccc tctcaatctt cggaataccg ctgaaaccgg cgaacagaag
     1141 gccgttcact acgccggttg cgctggtgtc cagggcgaca cccccttcgg gtaacgcggt
     1201 accccggttt actgtttcaa aacggacagg aaatggcacc gtctgggttg gaggaaggaa
     1261 gaccggtgtt agaaacgttg tgtcaaaaat gaggcggaag gttactcccg cgaccgggaa
     1321 cgaatcattc cgtacataga tttgtacgat aaccgcggtg tcgcccggga ggccgaacgc
     1381 gtccagaatt cgcagcgtat cgacggtcga ttgctgcgcc tgaacggtac ccgagcaaac
     1441 cgcccagcca atcaaaagtg taaaactgaa gagaagaatt ctgccagcgg cgacgcgcct
     1501 gcgtcggccc tgccgtactg ccggttgcgt atccatctat cgcagaagcg tcatcttgcc
     1561 gattgcctca ctctgttcgg tgattaggcg ggaaaagtag acgccgctag ccaccggctg
     1621 tccgctgtcg ctggtgccgt cccacttgag ttcgtggcgt ccggtggaga gggtcccgtc
     1681 aacgagcgtc ttcaccttct ggcccagtat gttgaagatc gagagattca cccgaccccc
     1741 ctgtggcaga cgaaaggcaa tcgtggtggc cgggttgaac gggttcgggt agttttcaaa
     1801 aaagtcgacc ggcgtaacgg aagtcaccgc tcgccccacc gcggtttcca aaagatctcc
     1861 ggaagaatca cagcaggccg cctgcgagat ctccggcgcc ctggctcccc gcagaggaat
     1921 gctgagcacc tctgtacagc cagcgggaag ccgccggccc tgggaactgt agagcaccaa
     1981 ccgcagggcc gaaccgacgt tctggctggc aagcgtaaag tcggggtcag ccaagcggag
     2041 ttccggttgc cctaccgtgg cgggaacgtt gccgaattcc aaaaacagtc cgctcaccgg
     2101 caccggggcg gccagagaaa gaatgagtga gtcgttgcgc accagaggtt ggacggtcac
     2161 ttgcgcggcg gcagccaccg cattcttggc gaagccgcca gtctgtcctc cccccccttc
     2221 cagaatccgc cgaatgagcg ccaccagatc gcccaccgtg gccggtatac cgtcgccgtt
     2281 aatgtcggag ttcacccgct gctgcgcgtt cagcggaaac gcccccggat tcaaaaagaa
     2341 gttgatcagc cggatcgcat ccccgatgtc aaacgcggca ccgttcaaat tgatgtcgcc
     2401 gaccaggggc gtgccgggcg tcaggatcaa aaccgcgccg tcctggaagg tgattgcgct
     2461 ctgcgcaatg agattcccgc gcggatccga cagcacgttg tcgctgggtg cgcggaaggc
     2521 gaacgtcacg ggaatggcaa aaccggccag attgacgtcg cgcgtaatat ggaaagagag
     2581 aaaagccacc gggccgatgc cgggtgccag aggcggcgta acgcccgggg cgttggtgtt
     2641 ggcgatgccg acaatgcgga tatcccccgc gttgcctccc gcattcggga cgacggagaa
     2701 taactcccag tcggccaggc gcgaaccggt tcgggtgacc gatgagaggg tcaaagccga
     2761 aatgtcgtaa ttgatcaaaa ggtccatccc gcccacggaa tcctgattcc ccagaaccac
     2821 cggtaccacc acgtcgccgt cgttgtatcc gctggcggtc aagacctgca accagaacaa
     2881 tgttgccgtc tgcaccgcca ccaggacgct ttcgacgtcg ccgccgccgt gggggtcctt
     2941 tgcccagaac accacccagt agcgcccggt gtcgcgggcc gcagtccacc agcggaaaag
     3001 ccagggattg ttgccggtaa aactggctcc cggcggcagc cgcagaaccg tcaccgtgac
     3061 cgggtccccg ttcgggtcgt tggcggtcac ggtaaaccgg gtggaatcgc ccatgaaaac
     3121 atgaatgcta tcgggaaccc ctaaccgggg ggggcgattg gtgaattgtt ctcccgccgg
     3181 ggcaccgacg gcgcacccga gaaccactgc ggccacgagc acgctgaccc gaccggagcg
     3241 tatcttccct gtcatcgttc gcaactgtaa aagcttatgg cgcaaatgcg ataccgattt
     3301 gccggacaaa acgatacctg attgacctgg gaaatttttg aacagtgcca ataaaaaagc
     3361 agttcgctcc gcctttcggg agaactgctc acccacactc catccgcacc ctgtcgccaa
     3421 gtcacctcgg cgagcggcgg tccgtgcctt gcgggtcgaa atcgactcgt accgagacgg
     3481 attctcaccg gcgcggcact gctctaccga aacactacgc tttgcgtcgc tatgcatatg
     3541 cgcaagaagg gacagacaac ccacgcggct ctgtccgtat gttccttacc aatcttgcgc
     3601 gatcgacttt cggtgtacct cccctggttt aagttttttt cgataggaga gtcccccgca
     3661 cgggggactc tccctatcgt tggatccgtc cgcctaaggc gggttacttc atcagcacca
     3721 tcttcttggt gtccgaggag ttcccggcta cacctttgta gaagtagatg cccgaagcca
     3781 ccttggcgcc tgtgttgtcg gtgccgtccc aggtaatggt cacgtcacca gcgtcaccgt
     3841 ggcccgtgaa actcttcacc acctggccag ccacgttgta aatggtcaac gtgtagttgc
     3901 tggctgccgg cagtgacaga gtaatccggg tcgacgggtt gaacgggttc ggcacgttct
     3961 gcgacacggc gaaaccggat ggaaccggct tgcccaggcc gctgatcgcc accgggtacc
     4021 cgtcgtagga gctggcgtca ccatccacca gggtcaggtt gccgtcggtc acgatgttca
     4081 acacggggct ttgacccgcg tcgatcttca tgccacgctc gtggctgtag atcaacaccc
     4141 gcagctggcc gccctcctga ttgaacttga cgttcatgtc ggaggcggtg cccacggtgc
     4201 ggacgtcgga taccgtgccg gtggtcttga agaccataag cgcgccaccc aggtccacgc
     4261 ccgcgttacc ctgaatgctg tatccgtcgg cggtccgtgt ggtggcaaac tccaccttca
     4321 cgtcgcccgg aacaaccttc gggaagggct gcgcgtcacc ggtgaggatg cggatcaagt
     4381 agaccaggtc ggccaccgtc aaggcaaacg catcgttgtt gacgttggag gccgcaatct
     4441 gcgcctgccg cctttgcggg tcggacgaga acacactcga gccttgcagg aagtagttct
     4501 cgtagagcac cgcgtcggcg atttcgttgg ccagaccgtt caggttcagg tcaccgcgcg
     4561 cgtcgatcgt gctgtcggcc gggatgcaaa tcttcccgtc gtgcaagcag atgaacttct
     4621 tgctccgcgg cttgttcggt ccgccggaag agacgcagtt ggaaacgtct accccgaggc
     4681 cgaattcagt cgccagatca aggcccacac cctcggcgtt gtaaaccgag tcgaccgtcc
     4741 acagctggtt accgctgcag tcggagatgg tgttgtcgtt gcaatcccag aaccaccagc
     4801 gaatcgggca gcaggtgttc tggaagttcc ggtcgcgggt cacctcaaac cagaggcaca
     4861 gccactgacc ggcgttgtcg gtctggcagg ctccccgcgg cgggtggtgc tgggccgcgt
     4921 cgttgatgtc ggcaagggcg atcacccgga tgaatccgga gggacagccg ttgccgcagt
     4981 tccccatcgg cccaaagcgg aaggtgaaga actcccactg acagcggatc aggaaggaac
     5041 ccatggtcgc tttctggaac gacaagcagg tcgggtcgta ggcgaacagg aagttgaagc
     5101 cacccacgca gctgccgttc gggatgtccg ggtcgctgcc attcaggggt tcgttcacgc
     5161 agaccggcac agtgtgcgtg ccgaggaaag cggtcacatg ctcgatgtcc aggcagaact
     5221 tctggcccac tacgaccagg aagttctttt cgcaactgtc cttagcgcca caagcatcag
     5281 tggcaacgat ccatgcgtgc tggggaccag cctcagccgg ggtcgggcag tactggtagg
     5341 ctccgcccgg ggtaatcgtg ccgacactcg gcgggacact attgcctaag ctgtaggtca
     5401 aggcatcgcc gtcggcgtcg gtcgtcggga acgtctcgtt gtcacaagag ttggcctgga
     5461 tctggatcac gccgcccggg caggtgatcg acggcgcctg attggtcacc gtcacgttga
     5521 agctgcactg gaccgtgtcc ttgccgtcgg tggctttcag ggtcactacg tgctggccaa
     5581 catcctcaca agatgtctgg aaccggtagc ggaaaggccc agggggcgat cccgtcactc
     5641 cacccggccc ggatgccagg aacacgttca aaggactcgg acaggcgtcg tccgaagccc
     5701 cgatcggcac gtcgatcaac gcgccgtgga gcacgctctg gttcgggggg caggcgagca
     5761 ccggcgggtt ctgggtaatg gtcaaggtaa agcagcaggt gtctgcgttg tggtgcaatg
     5821 ggtcactgac cgcagcgcac acctggtaaa ccccgacgtc accacaaaca gtagccccgg
     5881 ctcccaaagt ccagtcaccg ttagctgcca ctgctcccag cccgctgatc ttcacgaagg
     5941 tcaggggatc accctccggg tcggccccgg taaagctctg gcttgccggg ctcccgaaat
     6001 gcaccgaaat cggcgcggtc ccgtcgcagg tcggcggcag gttggcgatt gtctcgatgc
     6061 cttttaccac cttcggaaag ctcgtctgcg tcggcgggtg ggtgaacttc agcgtcttag
     6121 aggccgagag ccggttggtg tccagctgat actgccctaa gttgctgttg ttggtcaccc
     6181 aatacttgta ggcctcccgc cgcgcgccgg ccgggaacga accaccaccc gggtcgaccg
     6241 acaggacgaa ggcggagtcc gggctggtgc cgttcacgcc cgctaagctc actgtggcgc
     6301 tcaacggatc gatgttggcg ttctttttaa tggagtcaac cttgccgaac gccccgcccg
     6361 agagggtggt gatcactatg ggcagggata tcccgttgca ggccgagtcg ttggaaacca
     6421 tcaccgagat gaacccgatg ctcccgttgc tgaaggtttt ggactcgatg atgagcgagt
     6481 ccacaaaggg gcccgccccc gcctcctgcg ccaccgccac cccagcccac accagcaggc
     6541 tacaggcaat gacgaaaata ctgaaacgtc tcatcttgta tactcctttg gttcaaggta
     6601 cacccgtgct tgttgcacgt acacattgtt cacgcttctc cttcccctcc gttaaagtcc
     6661 ccgttatggg cgggactctt aacacgctca cctcctttgc agggctttgc agggaagttt
     6721 tacgcttggc tcacagacat cgcgttgcgt ttaggtcctc ctcacctcct cccgagtatg
     6781 gtcgccattt tacctccacg gtctgcgggg aacttagagt gggcatggct ccggcacccc
     6841 gaagaccacg tactgtatga gtgacacaat gtcgatgata tccaccacgc cgtccggcgg
     6901 acaacggacg tcgccattcg ccaggtcggg aatcggctcc tgggttccga acaccacgta
     6961 ctgcaccagc cgcacgacgt ccacgatgtc gatcacgccg tcgaaattca catcccccct
     7021 gagcgatcgg ggaattgtaa cggtccccgc attcttggtc agtccgcagg aagggccgcc
     7081 ggtgaagcag agaatgttgc cggtggtatc cgaaaagaag gtctgggtgg gatccagggc
     7141 aaaaccgacc tgtatcgccc gctgggaaaa agtatcggcg acggggttca cccgcaccgg
     7201 caatcgcaaa agcgtaccgt tgccgaaggg gatggcgttc gaattgctcc ccgctatcgc
     7261 cactacccgc aagaatatct ccgactgcca gcggccgtcg accttgctga aattgcgcgt
     7321 gcgggaacca ttcgtgtcca cgttggtgat gtagaccacg ggccggttga tcaccgtgtc
     7381 ccgcaacgta caggccggcg ggttcacaca cacgtaggcg gtttccgggg gcagggtatc
     7441 gacccggttg aagatgaact ccaccccctg cgggttgtca atggttaacg tcaacgtata
     7501 gccgccgatt ttcaaattgg gattggtcat cgcaatcctg atcttcgccg tctgatcgcc
     7561 gggccacaac accgtatcca ccacctgaac ctgcaccgtt agctgggcct gagccatccc
     7621 gctcactccc accaccgctc ctgccgtgag caggaaactt ctgactctca tccagacacc
     7681 tccctggtca ttcgggtttg cctttctttc tcctcacgta gtagcaaacc cggctatgca
     7741 acctccttgg ctttgggtaa ccctgccgtt ggatgaaaag tccactgcat actgtttaca
     7801 ccgtttgata accgaccccg cacggctgaa ggacaaaaca ccctcagtcg ccggtcgtaa
     7861 cagacctgtg accctcggga agggctaaag agcagggtat ttacaacgaa ctcgaatcgg
     7921 ctaagattca accctaatat aagaggccgg taaatcctgt caagtaaaaa aatcgttgcc
     7981 ccaccttttt tttcagtacg gtaattcgac cgaaacattg ccccccagcc tattgcaagg
     8041 agagtgccac ttttgggaca gcgcaccgcc tccgaatatc gattgcctat tctttacggt
     8101 gaattccacc gccgccatag aggattatga caatctagtt tattctcgat ttttcttggg
     8161 tataaccgat ccggtggcaa caaattgcac aaataaggca gatagtttca atagtcaaga
     8221 ggctgattgc cggtcgcgta tttttcgtct gagggggggc agctgaaact cagacggcaa
     8281 acagcccgac atccagcctc cgctcaccac aaccagagac tccttgaaat agtttcgccg
     8341 agttattgtt gagaaaggag gttttgtatt tgggggacca aagggccgtt gggcgccaaa
     8401 cggaggtatt tctgccagac gacccgggca ctgtctttct gtcccagtcc gtgatagacg
     8461 accccgaggt tgaagagcgc cacgaggtgc tgcggggagg cgttgagcgc gcgcaagaat
     8521 tcccctttgg ccttttggtt ttcgcccacg aagtggtagc acgctcccag atcaacgcgc
     8581 acgtccacgg cgctcgaatc gatggccaga gcgtgagtgt aatagagaat ggcttcgggg
     8641 aacctctgac tatccatggc ctgattcccc tggagcacca gttcgcggta ggccgtctgc
     8701 cgatcgttag cggaaagcag gcggttgatt tccgccagag cggcttcctc tcctgattgt
     8761 cccgcttttg acggaatggg cggacgggtt gaccgcaaat agatgaaaag aaaaatcacc
     8821 gcgccggccc cgagcagtcc gaagacgatc aaaggatagc gtggacggac gcctcgctgc
     8881 tgcggcttcg ttttttgttt ttttgaggag gtgctcgctg catccgcgcc gggttgggcc
     8941 tggggtactg gcaagggaac cccgcaaccg gagcagacgg aggcctcccg ggagttgagc
     9001 gtttggcacc accggcagcg gaggttctct ggcaggggac gaccgcagcg gttgcaaaac
     9061 cggctgccgg cagaattggc cgcctgacag gccggacagg tgacgcttga attttccata
     9121 cccgagaccc aaaatagact cgacaaagag acttgacaac agggaataca ctacatattt
     9181 tatcgctttg agggagggca gcccgagagg ttgccctttt gtatttgtgt gagcccttgt
     9241 tgtagatgag agtgaaggta ccgccgcact ataaccctaa cccctcaccg gagggaagga
     9301 gtaggtatgt tgcttcgcca aaggttactg ccggtcctgc tgttcgccgt gagcgtgtgg
     9361 gttgtaacgc ccgcaaaggc aggtgcggtt gatcaccaca tcactatgct ggcccacgtg
     9421 gtggatgaca ccatgcgcgg ttgctggggg tatatcgact ccttgggtcg ggaaattgct
     9481 ttggtctgtt cttacagcaa cctcgatctg tacgacgtaa ccgacccgac caatccaatc
     9541 ctgcgaaaac gagtacccca gatcgctgat gccactgacc tcaaggccgt gcgcccttac
     9601 cacgacagca tcgcctacgt cggcaaccag agcgggctga tgcaaatcgt ctacatctgg
     9661 aacgccgaca atgcgcagac catcgggagc tttgccaccc cgctcaccgg cggcaaccac
     9721 accatcgaga ttttcgggga ctatatgtac ttggggatga acggggccag cgaccggcgt
     9781 attcagatct ggagtctggc caatcccgcg gccccgacca aaataattag cctggctaat
     9841 cccggaaccg gcttcaacga cggtcacgat tgctacgtgc atggcgatac catctacact
     9901 caccacctca ccgccggttt caaggtgtgg aacatcgcca acaaagctgc cccggtcggt
     9961 ctggcaaccg tctcctatcc aggcgtcttc caacatgcca gctggacttc ttccgacggt
    10021 aaaattctct acaccgatga cgaaactgat ttcggccggt tgcgggtctg gaatgtgagc
    10081 gacctgcgcc agatcattca actggcgaac tacgccccct ccgccaatgc cgggggtgtg
    10141 gtgcacaacg tttacctttt ccagggagat gacctggcct acaccggctg ctacgaggaa
    10201 ggcgtacgga ttctcgacgt ctccgacccg acctacccga ttgaagtggg cggtttcgac
    10261 acctcaccgg gcaacaaccc cccgggtccg ccttcggcac cgcaggaagc ccgcgacggg
    10321 gtctgggacg cctacctttt tccttctgga acgctcattg ccacggaccg caaattcgga
    10381 ttctggactt tgcggtttga cggctcccgg gcggggcgga ttgaaggcac agtcaccaat
    10441 agcgtcaccg gtcagccgct gccggacgcg aaggtgaaac tgatcaactt aaccaacctg
    10501 cccaacgttc ccagtgtgct caccggaacc gacggttcgt acggcatccg caccggcagt
    10561 gtcctgcaga acgtcgaagt gagccgcttc ggattccgga ccgacaccgt gcaggtgacc
    10621 ggcatcgtgg ccgatacggt gccgctgaac gtggccttgg atcccgttcc ctacggcacc
    10681 atttcgggtc aggtgacccg gtcggccacc ggcgacccga ttgcgggcgt catcgtcggc
    10741 ctggcgggcg aaccctacca ctccgacacc accgacggtt cgggcaacta ctcgatcaac
    10801 tttgttccgg ccgattcgaa cttcttgatc gaaacctgga agtggggctt ccagagcgct
    10861 caggccaccg tactggtcac ggcgggaggg aatgccaccg ccaactttgg cttgcgcagc
    10921 ggttactcag atgacttcga acacgacctg gggtggacgg tcggggatgc gggtgacgac
    10981 gctaccgctg gtttctggac ccgcgtggag ccctttggga tgaaaaattt cgccaactcc
    11041 accgtgcaac ccgataccga tcacactgcc ccgcgcggcc agcgctgttg gattaccggg
    11101 caggggcgct tcggcatcgt ggccaacgat caggacgtgg acgacggcaa aaccaccctt
    11161 ctttccccga tgatggacct gtccgggttt tccacaccgg tgctttcgta ctggcgttgg
    11221 ttctcgaaca atggcgacca caatcctgac gaggacaccc tcgaggtctc tatttcctcg
    11281 gatggtggta tctcctgggt tgttctcgaa cgcaccaccg ctaccgagaa ccgctggacc
    11341 tccaagcaat tctgcctcac ctcgaaaatc aatctgacga gcaacgtccg tttccgggta
    11401 gtgatctctg accgccgggg gccctccttg gtggagggtg cttttgacga cttcgcggtg
    11461 ctcgaccggg ctgcgggtga tatcaacaac gaccgcactg tggacatcgc cgacataacg
    11521 caactcatca ctcacgtggt gttcggtacc cagccaccca atccactctg ctttggcgac
    11581 ctcaactgcg acggggtgat cgacatcgtc gacgtcgtcc agctgattct cttcgtcgtc
    11641 ttcggcgctc ctgcaccctg caccccgtga ggaaaaaaga agcagaaacc cccgccgaaa
    11701 gttaccgagg cgggggtttt cttttgggaa ttttcatgag gaaacccctg gggaaaagcc
    11761 ggcctcaaaa aaacttgaca aaacgacctt ctatttcgta ttctttcatc ggaaattact
    11821 cgcccgccgg agatagacgg aaaatgagaa aattaaccct ctttaccctc ctcaccctcg
    11881 gagctcccgg cttcttcctc tgctccaaga agcccaccaa cccaaccccg ccggtggtgg
    11941 tggaaaagcc ctgcgccgat ctggattcca ccctcacttt cacctgctgt cggcagtggg
    12001 tccgtgatcc tacctggtcc cccgatggaa atacgatcgc ttacgcagac ttagagatca
    12061 actggggcct gcatcttgtc aacccggacg ggagtaatga ccatcttatc gttccggccg
    12121 ttggggtttt cgaactggat ttttccagtg acgggcagtt gatactcttc ctttggcttc
    12181 gcacgaatca accaccggga tcaaccggta tttataagat tcgaacctca gggaaagata
    12241 caacaaaaat ttcaagcact tatgacgatt tctacccccg ttggtcgccc gatgataaat
    12301 taatcgcatt cgagagggac atatcttcag gcgatcctca gaatggggtc tacttgatga
    12361 acgccgacgg caccggagca cgccagatct ttccgttagg ccaggttcca gactggagcc
    12421 ctgatggacg cttggtggtt accaaaggcg ctaacttgta catcatcgat actcttggga
    12481 atgtcttatt gaagcttact aacttcctcg ccgactccct ggggcgggga ataagggcgg
    12541 gctcaggtcg
//
LOCUS       DBNGZD010000434         9484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1801883, whole genome shotgun sequence.
ACCESSION   DBNGZD010000434 DBNGZD010000000
VERSION     DBNGZD010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1801883"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..527)
                     /locus_tag="AC16GM_11280"
     CDS             complement(96..527)
                     /locus_tag="AC16GM_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924290.1"
                     /translation="MDSYVHLIALSNKGASVYLIEMPYKSWQWLAEGLTQLGGMGFEE
                     LGKKNSIWTPREVPTPWAMRAPPIDSLLGRELKRYLSELWTEGWYGRYRQKGIILPRF
                     EGVSAKLLYHYPSGIYVNYDIEKVYYSRQFAMGKVPFQPPM"
     gene            complement(764..2785)
                     /locus_tag="AC16GM_11285"
     CDS             complement(764..2785)
                     /locus_tag="AC16GM_11285"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR2924291.1"
                     /translation="MARTGKNLSHLIFLNVSILWGLVPFRPNTASAAESGTLVPQLSV
                     WRYFADAQYPDSLWHRPGYDDGLWPSGPGILGFGEVFIATTVPFGPDPANKYITTLFR
                     HSFQLNVDPAQITQLTLSANYDDGFVAYLNGVEVTRQSMPAGTLQYSTLALSHEGGGY
                     EPIDISSHINALVQGTNILAVEVHQTSPASTDLVWDAELSFSTSQIAFLWSGGITPTS
                     ALVKAKLLQDSGVARLAVSLLPDFSSPLYSNFDTALTTLNNGVVSFEIDGLIPTQRYH
                     YALEVNGVLDTEKVGRLRTFPQDTGSFTFAFASCALTGSNHPVFETIRSLDPLFFFHL
                     GDMHYQNIAVNDQNLFRQAYETVLASPNQSRLYRDIPLAYIWDDHDFGPNNTDSTAPG
                     RLAARLTYQEYVPHYPLAAGSGDVPIYYAFTVGRVRFVVCDSRSARSPATALDNPSKT
                     MLGVAQKAWFKQELLNARSNYALIVWVNSLPWIGTTGDDGWYLYTYERMELANFIKNN
                     GITNLCMISGDAHMLAIDNGTNSDYATGGGAGFPVMHAAALDQSGSVKGGPYSEGAYP
                     GGGQFGLMTVIDAGDSVRVEWSGRNSLNQEIVRYAFTLPPGVMKGDLDGDGAVNIVDI
                     VIMINYVVFGDPIPEGTSNADLTGDGLVDLTDIVCLINHIVFAIPLPCL"
     gene            complement(2928..3188)
                     /locus_tag="AC16GM_11290"
     CDS             complement(2928..3188)
                     /locus_tag="AC16GM_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2924292.1"
                     /translation="MPFTVYILQSQSTGKIYIGQTTNLERRIQQHNDPNDWRTKHTKR
                     NPGPWRLAHSEVYATRSGAMQRERALKSGQGRTWIQRQVLGK"
     gene            complement(3213..3288)
                     /locus_tag="AC16GM_11295"
     tRNA            complement(3213..3288)
                     /locus_tag="AC16GM_11295"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3253..3255),aa:Asn,seq:gtt)
     gene            complement(3421..3927)
                     /locus_tag="AC16GM_11300"
     CDS             complement(3421..3927)
                     /locus_tag="AC16GM_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR2924293.1"
                     /translation="MNKIAIELTQGNIADVEADALVNAANNRLVMGAGVAGALKRQGG
                     RKIETEAMQKGPIPIGEAVATGAGKLIAKYVIHAAVMGMDFRTNADYIRQATLNSLKR
                     AEELKLQTIAFPALGTGVGGFPKEECARIMLGEISRFPANSLERAIFVLYDETTLETF
                     RRELPKER"
     gene            complement(3924..4481)
                     /locus_tag="AC16GM_11305"
     CDS             complement(3924..4481)
                     /locus_tag="AC16GM_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR2924294.1"
                     /translation="MTEFFHNLDTQLFIFLNATLANPVFDRVMPVITNSFYWRLPLAA
                     ILLYLLIWGKKKGRITVLLFVLTAVISDQISSHLIKSFIHRLRPCHVVPQVHLLVNCV
                     HSYSFPSSHAVNSFAFAIMFWRRYPQSKYFFFPIVAIIAYSRVAVGVHYPFDILGGAL
                     IGIGVGLGLFWLYRKASRRWRWEGV"
     gene            complement(4577..4963)
                     /locus_tag="AC16GM_11310"
     CDS             complement(4577..4963)
                     /locus_tag="AC16GM_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR2924295.1"
                     /translation="MKRQNVSSGSPFESIIGFSRAVRVGHHVLVSGTAPIGADGKTVG
                     IGDAYVQTKCCLEIIAQALEKAGARLEHVVRTRTFLVNIDDWEKVARAHGESFATTRP
                     TSTFVQVSRFINPEWLVEIEADALIE"
     gene            complement(5040..5684)
                     /locus_tag="AC16GM_11315"
     CDS             complement(5040..5684)
                     /locus_tag="AC16GM_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HIR2924296.1"
                     /translation="MTATKDTYQTIGAPCSARLKIQGSIFFARAVPIASVTEVTPFLT
                     SLKKELHDATHHCWAYKLDDGVSARVKWSDAGEPKDTAGRPILQAIESRDLTNLLVVV
                     SRWFGGTKLGKGNLSRAYRQAAEQALANAPIVTGCWRTRLRLSVSVEQVSAVYAVAGR
                     FAGRVQVEASDNGGRFTVAVPRSETEKFLELLMERTAGKIQFEYTHAGKAKKRT"
     gene            complement(5689..6582)
                     /locus_tag="AC16GM_11320"
     CDS             complement(5689..6582)
                     /locus_tag="AC16GM_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014533.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR2924297.1"
                     /translation="MTYSEQTYVKALRERGKVGPKTFQMLLYHFGSPEAILNTPWEEI
                     AALPRLNEKRAKEILGARDWLEDAALELNALAGDQIRLMTFLEDGYPERLKAISDPPP
                     LLYLKGEFPVPSERFVAVVGTHEATAEGIAAAVQFGKVLAGAGAVVVSGLARGIDAAG
                     HVGALKAGGKCYGVLGSGFGQIYPKEHDSLASEVAKNGALISEYAPGVPVNVGQLMAR
                     NRIVVGLASAVIIVELPGPDGAEQNNSGTLNAMERGREQGKPVYLYDPQKHLSAEQLH
                     QWEAQSLIGPEQLSELLPYLI"
     gene            6727..7641
                     /locus_tag="AC16GM_11325"
     CDS             6727..7641
                     /locus_tag="AC16GM_11325"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HIR2924298.1"
                     /translation="MAQRKRTPAIIVHGGAWDIPDDLVKPHLDGCRRAVEAGYSLLRE
                     GASALDAVVAAVKVMEDDPAFDAGVGAFLNAEGEIELDASLMDGRTLGCGAVAGVKTV
                     KNPIELARLVMERTEYVMLIGSGAEAFARQQGIPACRFEDLLVPRERERWQEFKRRKN
                     FSARGVFATKPHGTVGAVAVDSEGNLAAANSTGGTPNKLPGRVGDTGQIGSGTYADNE
                     KGAACSTGWGEPIIKIVMAKAICDRMAKKTAPKAAINQALNELRKRVGGFGGAIAIDR
                     TGRVGLAFNTPRMAFAYSNVQGDIVTGI"
     gene            complement(7643..9016)
                     /gene="rsmB"
                     /locus_tag="AC16GM_11330"
     CDS             complement(7643..9016)
                     /gene="rsmB"
                     /locus_tag="AC16GM_11330"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR2924299.1"
                     /translation="MCKIAPTVRSSRSSAGLKSRQIALELLLGLERSREDFFESLAAH
                     RLLAPLVPVDRRLVQELVSGACKMQRRLDFILNQFLTQKKTKLDPLVRSILRLALYQL
                     EFLTRVPDFAAVNSAVELARERALGRKADFLNGLLRSYLRNREKLAYPERERNPNEYL
                     GIYYSYPDWLVARWVDEFGPEETEKLCAYGNRKSSLTLRVNTFRTSVEKIIAQLEQVG
                     VRVMPGKFFPQFLTPKSEYRFFDSPWFKNGFFYVQDESAAAAVHLLDPKPGETIWDAC
                     AAPGGKATHVAELTGDTGTVLASDLSLRRTKLIKENSTRLRLNSVIAVVADGLSYHPK
                     GMRKILLDVPCTGLGTVARNPDLRWHKKPEDIPRLAQLQWELLTNAVMSLPVGGVLVY
                     SSCTLTREENQELMEKFIHTHPRYRLEPAGEFVPPELVDGFGYVAILPQKHGIDGAFA
                     ARFRRIW"
     gene            complement(9009..>9484)
                     /locus_tag="AC16GM_11335"
     CDS             complement(9009..>9484)
                     /locus_tag="AC16GM_11335"
                     /inference="COORDINATES: protein motif:HMM:NF021610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HIR2924300.1"
                     /translation="RLSEQLPPGFTVKNGRSILNKTFSLSQVINAARYAVDFTGDVPP
                     ERTDGWPEKITDILAARSWEITRRQTERTSEVDIRPHIYDLKWEKRAETTRINMLVGL
                     GTGGYAKPQEILRFGLGLSEEEIAGLVIRRTNLYVRRQARLDPGQFEWLSPLEVV"
BASE COUNT         2071 a         2635 c         2698 g         2080 t
ORIGIN      
        1 cctgcccctc taccttgcgg aatatgtatg gcgctacaat caccggcgtt tatctttccg
       61 acaacagacc caaacgctca tgggactgct gagactcaca taggtggctg gaatgggact
      121 ttacccatag cgaattgtcg tgaatagtag accttttcaa tgtcatagtt tacgtagatg
      181 ccggagggat aatggtagag caatttggca cttactcctt caaatcgagg gagaataatt
      241 cccttttgcc tgtaccttcc gtaccagcct tcagtccaga gttcgctgag atagcgtttt
      301 agttcccttc cgagaaggga atcgataggc ggagctctca tcgcccaggg agtggggacc
      361 tctcgaggtg tccagatgct gttctttttt ccaagttctt caaagcccat cccacccagt
      421 tgggttaatc cctctgccaa ccattgccaa cttttgtaag gcatttctat caagtacact
      481 gaggctcctt tattagacag ggcaatcagg tgtacatacg agtccataca ctctccaccg
      541 atatcctctg caactcaaaa gatttcggcg tttccacgag tcactcctaa aagcgtgaat
      601 gtctgaacgc aatttgtagt tgcgtgagca tcaaaacgaa acagaaataa agaacaaatc
      661 acaaggccca atgagaccaa ttttttactt aaacatacag agttcatatg agtttctttg
      721 ggctcccact acagggctcg aacctgcgcc cttatgcttc tctctacagg cacggcagcg
      781 ggatagcgaa gacgatgtgg ttaatcaggc aaacaatgtc ggtgaggtct acgaggccgt
      841 cgccggtcaa atcggcgttg ctcgtgcctt cgggaatcgg gtcgccgaac accacgtagt
      901 tgatcatgat gacgatatcc acgatgttca cggcaccgtc gccatccaaa tcccctttca
      961 tgacgccggg gggtagggtg aacgcgtagc ggacaatctc ctgattgagg gagttcctcc
     1021 cgctccattc tactctgacc gagtctcccg catcaatgac ggtcatcaat ccaaactgcc
     1081 cgccgcccgg gtaggcccct tcgctgtacg gccccccttt cacggatcca gactggtcca
     1141 aagcggcggc atgcatcacg ggaaaaccgg ccccgccgcc ggtggcgtag tcgctattgg
     1201 taccgttgtc aatggccagc atatgggcat cccccgagat catgcagaga ttggtgatac
     1261 cattattttt gatgaaattg gccagttcca tgcgttcgta ggtatagagg taccagccgt
     1321 cgtcaccggt agtgccgatc cagggtaggg aattgaccca gacgattaac gcataatttg
     1381 acctcgcgtt cagcagttcc tgcttgaacc aggccttttg cgccactccc aacatagtct
     1441 tggagggatt atcgagtgcg gtggccggac tgcgggccga gcgcgagtcg cagacaacga
     1501 aacgcacccg gccgacggtg aaggcatagt aaatgggcac gtcgccggac ccggccgcca
     1561 gagggtaatg gggaacatac tcctgatacg tcaggcgcgc ggccagccgg cccggcgcgg
     1621 tggaatcggt gttgttcgga ccaaaatcat ggtcatccca gatgtaagcg agcggaatgt
     1681 cgcggtacag ccgggattga tttggagaag ccaaaaccgt ttcgtaggcc tggcggaaga
     1741 ggttttgatc gttgacggca atgttctggt agtgcatatc gccgaggtga aagaaaaaga
     1801 gcggatcaag ggaccggatg gtttcaaaca cagggtggtt tgagccggta agggcgcagg
     1861 aagcgaaggc aaaggtgaac gagccggtat cttggggaaa ggtgcggagg cggcccactt
     1921 tttcggtatc cagcaccccg ttgacctcca gcgcatagtg gtagcgctga gtcggaatga
     1981 ggccgtcgat ttcaaatgaa acaactccat tgttcagggt agtcagcgcg gtgtcaaaat
     2041 tcgagtagag aggagaggag aaatcaggga gtagactgac cgccaggcgg gcgacgcctg
     2101 agtcctgcag gagtttggct tttacgaggg ctgaggtggg agtgattccc cctgaccaga
     2161 ggaacgcaat ctgagacgtg ctgaaggaaa gctcagcatc ccacaccagg tcagtacttg
     2221 cagggctggt ttgatgcacc tccaccgcca gaatgttcgt tccttgcacc agagcattga
     2281 tatgactgga aatatcaatc ggttcataac cccccccttc gtgggaaagg gctaaggtgg
     2341 agtactgcag ggttcccgcg ggcatcgact ggcgggtgac ttccacgccg tttaggtagg
     2401 ccacaaagcc atcatcatag ttcgccgaca gtgttaactg agttatctgg gctggatcga
     2461 cattcaactg gaaggagtgg cggaagagtg tggtgatgta cttgttggcg gggtcggggc
     2521 cgaagggaac ggtggtggct atgaaaacct ccccaaatcc cagtattccc ggtccggaag
     2581 gccagaggcc gtcgtcatag cccggccgat gccacaggga gtcgggatat tgagcgtccg
     2641 caaaatacct ccagaccgaa agttgaggaa ccaaggtacc cgattctgcg gctgaagcgg
     2701 tattcggtcg gaatggcaca aggccccaca ggatcgagac gttcaaaaat atcagatggg
     2761 aaaggttctt accggttcgt gccattagag aattcgacga tattgttccc acggcaggat
     2821 tcgattcccg ccgttggcgg gattaacagc cactttcggc tcgtgccaat tagctcccgc
     2881 ggcaggactc gaatccgcct caggcggatt aacagccact cgctgtttca tttcccgaga
     2941 acctgccgct gaatccaagt tctgccctgg ccagatttca gcgcccgttc cctttgcata
     3001 gccccagagc gggtggcata gacttcagaa tgagccagtc gccagggtcc cgggtttctc
     3061 ttcgtgtgtt ttgtccgcca gtcgttcggg tcattgtgtt gctgaatccg tcgctctaaa
     3121 tttgtggttt gaccgatata aatctttccg gtggattggc tctgcaggat atagaccgta
     3181 aagggcatct gtccatccag gggaatgaac tttggctccc gcggcaggac tcgaacctgc
     3241 gacctggtgg ttaacagcca ctcgctctac cgactgagct acgcgggagt gaattcactt
     3301 ccgatacggc agtataataa attatcggtt atttgaacaa cgggatctcc cgcggcagga
     3361 ctcgaatccc gcctgaggcg gagggggcgt aaacttttcc actgtccctg accgttctcc
     3421 ttacctttct ttcggcagtt cccgccggaa ggtttcgagc gtggtttcat cgtacagtac
     3481 gaagatcgct ctctccagcg aatttgccgg aaatcggctg atttctccca acataatccg
     3541 cgcgcactct tccttcggga atccacccac gccggtaccc agagcaggaa aggcaatggt
     3601 ctgaagtttc agttcttcgg cgcgctttag agaattcaga gtggcttggc ggatgtagtc
     3661 ggcattggtc cggaaatcca tgcccatcac tgcggcatga atcacgtact ttgctatcag
     3721 ttttccggcc ccggtcgcta ccgcctcgcc gatagggata ggtcccttct gcatcgcctc
     3781 ggtttcaatt ttcctgccac cttggcgctt gagcgctcca gccacgcctg cgcccatcac
     3841 cagccggttg ttcgccgcgt tgaccagggc gtcggcctcc acgtcggcga tgttcccctg
     3901 ggtcagttcg atagcgattt tgttcatact ccttcccatc gccaacgacg actcgcttta
     3961 cgataaagcc agaagagtcc caggccaact ccaatgccaa taagagcacc gcccaggatg
     4021 tcgaacggat agtgaacccc caccgccacg cgcgagtagg cgatgatcgc gactatcggg
     4081 aaaaagaaat acttcgattg cgggtagcgc cgccagaaca ttatggcgaa cgcaaacgaa
     4141 ttgaccgcat gggatgacgg gaacgaatac gaatggacgc agttgaccaa aagatgcacc
     4201 tgcggcacca cgtggcaggg gcggagccgg tggatgaacg acttgatcag gtggctggaa
     4261 atctggtcac taattactgc cgtgagaacg aaaagaagga cggtgatccg tcctttcttc
     4321 ttcccccata tcaagagata aagcaggatg gccgccagag gcagccgcca gtagaatgag
     4381 ttggtgatca ccggcatcac ccggtcgaaa accggattgg cgagggtagc gttcaaaaag
     4441 atgaaaagtt gcgtgtcgag attgtggaaa aattcagtca atgcactacc ctgatcattt
     4501 ccgcttgaag caattattca gatttcccat tccgcgactc tcgcaccggg gtctgatgaa
     4561 agcgggtaca aattttttac tcaattagtg catccgcttc gatttcgacc aaccattcag
     4621 gattgatgaa gcgacttact tgaacaaatg tggaagtcgg tcgcgtcgtc gcgaagctct
     4681 ctccatgggc acgcgcaacc ttttcccagt cgtcaatgtt cactaagaag gtgcgggtgc
     4741 ggaccacatg ttcaagccgg gcaccggctt tttccaatgc ctgtgcgata atttccaggc
     4801 aacatttcgt ctgaacgtag gcatctccaa ttcccaccgt ctttccatcg gcaccaatgg
     4861 gggctgtgcc tgaaacaaga acgtggtgcc caacacgcac ggcgcgggaa aaaccgatga
     4921 tgctctcaaa aggagagccg gaagacacat tctgccgttt cattctggtt gctcctcctc
     4981 tccataagcg agttgacttg acagcatagc aaaattcatc cggtagggcg ctccgtttcc
     5041 tatgttcgtt ttttcgcctt tcccgcgtgg gtgtactcga attgaatttt accagcggtg
     5101 cgttccatca aaagttccag aaatttttca gtttcactcc ggggaacggc gacggtgaaa
     5161 cgtccaccgt tgtccgatgc ttcgacctgc acccggccgg caaagcggcc tgccacagcg
     5221 taaacggcac tcacctgttc caccgaaacc gaaaggcgga gccgagttcg ccagcagccc
     5281 gtcacgatag gtgcgttggc cagcgcctgc tccgccgcct gccgataggc ccggctgagg
     5341 ttgcccttgc ccagttttgt gccgccgaac caacggctga ccaccaccag aaggttggtc
     5401 agatcccgtg attcgatggc ttgcagaata ggcctgccgg cggtgtcttt cggctcaccg
     5461 gcatcagacc attttacccg ggcagaaaca ccgtcgtcca gtttataagc ccagcaatgg
     5521 tgggtggcat cgtggagctc ttttttcaaa ctggtaagga acggcgtcac ctccgtaacg
     5581 cttgcaatcg gtaccgcccg ggcgaagaaa atcgagccct gaattttcag tctggcgctg
     5641 cacggcgcgc cgattgtctg gtaggtgtct tttgtggctg tcaattgact aaatcaggta
     5701 agggaggagt tcggacaact gttcgggacc gatcagcgat tgcgcctccc attgatgcag
     5761 ctgttcggcg gagaggtgct tctgcggatc gtacaggtag accggcttgc cctgctcccg
     5821 cccccgttcc atagcgttca gcgttccgga attattttgc tccgcgccat ccggcccggg
     5881 gagttccacg ataatcacgg cgctggcgag ccccaccacg atgcgattgc gggccatcaa
     5941 ttgcccgacg ttgaccggca ccccaggcgc atactccgaa atcaaggcgc cgtttttggc
     6001 gacttcggag gcaagcgaat catgctcttt cggatagatt tgcccaaagc cggaacccaa
     6061 gacgccgtag cacttgcccc ccgcttttag cgcccccacg tgtccggcag cgtcgatgcc
     6121 gcgcgccagg ccgctcacga ccaccgcacc ggccccggcc agcactttgc cgaactgcac
     6181 cgcggcggca atcccttcgg cggtcgcctc gtgcgttccc acgacggcga caaagcgctc
     6241 gctcggtacc ggaaattcgc ctttgaggta gagcaaaggc ggcgggtcgc tgatggcttt
     6301 caaccgttcc gggtagccgt cttctaagaa cgtcatcaat cgaatttggt ctccggccag
     6361 ggcgttcagt tccagcgcgg cgtcctccag ccagtcgcgt gcgcctaaga tttctttggc
     6421 gcgtttttcg ttcaagcgcg gcagtgcggc aatctcctcc cagggggtgt tgaggatcgc
     6481 ttccggcgag ccgaagtggt acaggagcat ctgaaacgtt ttgggcccca ccttgccccg
     6541 ctcccgcagg gctttgacgt aggtttgctc ggagtaggtc atcaccggaa cccgccgaag
     6601 atttgactca acatatgaca aaccataata aagagtcgtt tatttccggc tgcaagtcag
     6661 caacagtcgt tccaccagaa aatatcgctt gactttcatc cgaaattctt tttcctgaag
     6721 cgcttaatgg cccagcgcaa gcgcactccc gcgatcatcg tccacggcgg agcgtgggat
     6781 attccggatg acctggtcaa accgcatctg gacggctgcc ggcgggcggt ggaagccggt
     6841 tattccctgc ttcgggaagg cgcttcggct ctggatgcgg tagtggcggc ggtcaaggtg
     6901 atggaagacg atcccgcgtt cgacgcgggt gtcggcgctt tcctgaacgc ggagggtgaa
     6961 atcgaactag atgcttcgct gatggatggc aggacgctcg gctgcggggc ggtggcgggc
     7021 gtcaaaaccg taaaaaatcc gattgaactg gcccggctgg tgatggagag aaccgagtac
     7081 gttatgttaa tcggcagcgg ggccgaagct tttgcccgcc agcagggtat tcccgcctgc
     7141 cggtttgaag atttgctcgt cccccgcgaa cgggagcgct ggcaggagtt caaacgccgg
     7201 aaaaactttt cggcccgagg ggtctttgct accaaaccgc acgggacagt gggggcggta
     7261 gcggtcgata gtgaaggcaa tttagccgcc gcaaactcca ccggcggaac gcccaacaaa
     7321 ctgccgggcc gggtgggtga caccggccag atcggctccg gcacctacgc ggacaacgag
     7381 aaaggcgccg cctgctccac cggctggggc gagccgatca ttaaaatcgt catggcgaaa
     7441 gcaatttgcg accggatggc caagaaaacc gctccgaagg cggccatcaa ccaggccctg
     7501 aatgaattgc ggaaacgggt cggcggcttt ggcggggcca ttgccattga ccggacggga
     7561 cgggtgggtt tggccttcaa taccccgcga atggcgtttg cgtacagtaa tgtccaggga
     7621 gatatcgtaa ccggaatttg aattaccaga ttctccggaa ccgcgcggca aaggcgccat
     7681 caatcccatg tttttggggg agaatcgcaa cgtagccgaa gccgtcgacc aattccggcg
     7741 gcacaaattc acccgccggc tccagccggt agcgggggtg ggtatggatg aatttttcca
     7801 tcagttcttg gttttcttcc cgggtcaaag tacagctgct gtagaccaga acaccgccca
     7861 ccggcaggct catgaccgcg ttggtcaaaa gttcccattg cagttgtgcc agacggggga
     7921 tgtcttccgg ttttttgtgc cagcgtaaat ctggattgcg tgccactgtc cccagaccgg
     7981 tgcagggaac gtcgagcaaa atttttcgca tccctttcgg atggtaggag agaccgtcgg
     8041 caacgaccgc gataacagaa ttcaacctga ggcgggtgct gttctcttta atgagtttgg
     8101 tgcgccgcag actgaggtcg ctcgccagaa ccgtgcccgt atcaccggtt aactcagcta
     8161 cgtgagttgc cttgcctccc ggcgcggcac aggcatccca aatcgtttcc cccggttttg
     8221 gatcaaggag gtgcacggcg gctgcggcgc tttcatcctg tacgtagaag aagccgtttt
     8281 tgaaccaggg ggaatcgaaa aaacggtatt cggatttagg agttaggaat tggggaaaaa
     8341 actttccggg cataactctg acgccgacct gctcgagttg cgcgattatt ttctcaacac
     8401 tggtacggaa tgtattgacc cgcagggtca aagaggactt acgattgccg taggcgcata
     8461 acttttcggt ttcctccggg ccgaattcgt ccacccagcg cgccaccagc cagtcggggt
     8521 aggagtagta aattcccaga tattcgttcg gatttctctc tctctccggg taagcgagtt
     8581 tctcccgatt gcgcaagtag gaacgcaaaa ggccgttgag gaagtcagct ttccggccca
     8641 gggcgcgctc cctggccagt tctacggctg aattgacggc ggcgaaatcg ggcactcggg
     8701 ttaagaactc cagctggtag agagccagac gcaggatgct gcgcaccagg ggatcgagtt
     8761 tggttttctt ttgcgtcaag aactggttca ggataaaatc gagccgcctc tgcatcttgc
     8821 aggcgccgga aaccaactcc tgcaccaagc ggcggtcaac cggaaccagg ggcgctaaga
     8881 gccggtgcgc agccagagat tcgaaaaaat cttcccggct gcgctcaagt ccgagcagca
     8941 gttcaagggc tatctggcgg gattttaatc cagcggaaga acgggacgag cggaccgtcg
     9001 gagcaatctt acacaacctc caggggcgag agccactcga actgccccgg atccagacgg
     9061 gcctgccggc ggacgtagag attcgtgcgg cggatcacca atccggcaat ttcctcttcg
     9121 ctcaacccca acccgaagcg cagaatttcc tgcggcttgg cgtacccgcc cgtccccagc
     9181 cccaccagca tgttgattcg tgtcgtttca gcccgttttt cccacttgag gtcataaatg
     9241 tggggccgga tatcgacctc ggaggttctt tcggtctggc ggcgggtaat ctcccaggag
     9301 cgcgctgcca gtatgtctgt tatcttctcc ggccagccgt cggtgcgctc tgggggaacg
     9361 tcaccggtga agtccacggc gtaccgggcg gcgttgatca cctgcgagag cgaaaacgtt
     9421 ttgttcagta tgctccttcc gtttttcacg gtgaagccgg gcggaagttg ttcggagagc
     9481 cgcg
//
LOCUS       DBNGZD010000435         6013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1318625, whole genome shotgun sequence.
ACCESSION   DBNGZD010000435 DBNGZD010000000
VERSION     DBNGZD010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1318625"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /gene="aspS"
                     /locus_tag="AC16GM_11340"
     CDS             <1..1817
                     /gene="aspS"
                     /locus_tag="AC16GM_11340"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR2924301.1"
                     /translation="WTSDWKRTHTCGQLRQADGGKKVTLNGWVYRWRNHGGLAFIDLR
                     DRYGITQVVFNPKILGQEAVDRAGSFRAEYVIAVKGTAAIRPTGMQNPQLETGEIEVA
                     VESFAVLNESKTPPFEIADDSDATEELRLKYRYLDLRRKPLQENIRLRHRVAKAVRDY
                     FDSVGFYEIETPVLIRSTPEGARDYIVPSRVNRGKFYALPQSPQLLKQILMISGFDRY
                     VQLARCLRDEDLRADRQPEHTQIDLEMAFVTQDDVFSVVEGMFKHIFKVVRNEELPTP
                     FPRIRYVDAMNRYGSDKPDLRFGMELVDLTDLVQQSPFRVFSETVKNKGVVKGLAVKG
                     QAAMTRSQIDHWEETARKWGAKGLIWIVRTKDEVRSPLTKFVPKEEIDTLVKRLSLEI
                     GDLAFLVADRWHVTCNVLGRLRVELARQLGLIPQDQWNFSFVIEFPLFEYHPQEKRYN
                     AMHNIVTSPWAEDIPKLDAGFTSPLSLDHPDHPWGQIRANQYDLVLNGNEMASGGIRN
                     HRRDIQQKILNVLGMSDERAERMFGFLLTALEYGAPPHGGIALGLDRIVTLLTGSDSI
                     RDVIAFPKTAAAQSLMDGTPAEVDPEQLAELGIRIVKE"
     gene            1820..2788
                     /locus_tag="AC16GM_11345"
     CDS             1820..2788
                     /locus_tag="AC16GM_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR2924302.1"
                     /translation="MEDTTTRRIVLSDIDPRLVFGQHDAYLRIIESQFGVKIISRGQD
                     LTVEGEPSEVTQVEKVISDLITRIKANQEVNERYLLYAIAMVKENGAGPADQLQLNAL
                     ITSLSKEPVQPKTVGQKAYVQAMTQYDIVFSIGPAGTGKTYLAVAMAVAELKKKMVNR
                     VILVRPAVEAGESLGFLPGDIRAKVDPYLRPVYDALHDLLAGEKIRKLIEAGIIEIVP
                     LAFMRGRTLNSAFVILDEAQNTTNAQMKMFLTRLGNNSKAVITGDITQIDLNDKGASG
                     LVQIQKILTGIPGIKFVYLTEKDGVRHRLVQDIILAYERYENKKSK"
     gene            2888..3265
                     /gene="ybeY"
                     /locus_tag="AC16GM_11350"
     CDS             2888..3265
                     /gene="ybeY"
                     /locus_tag="AC16GM_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR2924303.1"
                     /translation="MVLAPFRPLVRSILKGEKRAGDITIVFITDERMADLNFRFRRKN
                     RTTDVLAFPLNDKLAANGRVQGEIYIALDQARRQAKEYGATLAEELKRLVVHGTLHLL
                     GYDHHRRAQARRMRDKEARYLFA"
     gene            3269..4522
                     /locus_tag="AC16GM_11355"
     CDS             3269..4522
                     /locus_tag="AC16GM_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR2924304.1"
                     /translation="MLILYLLLSLFFLFLGYAATAATAELYRDPQELEKLTADQKERR
                     FLKRVLARPQETLFVASAFRALLFLTLTVLTLEIQRLASAGLPGFSWVWYFAGFLALW
                     LFWLLTVEVFPKFRLLSGQPSKLIRYLWLVGPLVILFKPLSALLFPPRERRTHDPELE
                     EKKEEMVERALETLAETSGVDEPLIEQEEKKMISQILRLDTTAVKEIMIPRVNIVWVN
                     HTASFDEVKEIVKTSGHSRLPVCRETIDDILGILYIKDLIHAAIIEPTPDWSQYVRKS
                     YFVPESKKIDELLDELRSRKTHMAVVVDEYGGTAGIVTLEDILEEIVGEIHDEYDVGE
                     PEIQKVDDQTFRVTGTVNVQHLAETVGVDLPEEKFETVGGLIYDLVGSLPEEGKVVEH
                     AGLRFKVEKVVGQRIKKILVARTSH"
     gene            4766..4848
                     /locus_tag="AC16GM_11360"
     tRNA            4766..4848
                     /locus_tag="AC16GM_11360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4799..4801,aa:Ser,seq:tga)
     gene            4958..5929
                     /locus_tag="AC16GM_11365"
     CDS             4958..5929
                     /locus_tag="AC16GM_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924305.1"
                     /translation="MLRYQMKRRGMCLRWLAKANLIMGGIFLASAVKPAQAGPFVDSL
                     IVESKTFGPASQGAIEIFVSNDSAVTSIQLPLVIHSLSGGAFARIDSVKQLFTSPLPP
                     GTRTPVFRAFVTNNSGLGTYAVDSGGPLLVIHTGGQPNVPFFVRGVETIGNQAPILRC
                     SPGRTVYFGASVSLLPLEASDPEGDQLLFSLVGFSKIGGGGPPQNSPVVDVNTGRFFW
                     QTVSNATEVGTWETTLGVWDPLSFSPVTCTFTIEVTDLRGDVNNDGLWSDVVDIIELI
                     NLVVFQTSITSENPNSADVNCSGVVDLEDIVALISFVVFGAPAPCRG"
BASE COUNT         1339 a         1615 c         1756 g         1303 t
ORIGIN      
        1 tctggacttc tgactggaag cgcacgcaca cctgcggcca gttgcggcag gcggacggcg
       61 gcaaaaaagt gaccctgaac ggctgggtct accgctggcg caaccacggc ggcttggcct
      121 tcatcgacct gcgggaccgc tacggcatca ctcaggtcgt cttcaacccc aaaattctgg
      181 ggcaggaggc tgtcgatcgg gccgggagtt tccgggcgga gtacgtcatc gcagtgaaag
      241 gcacggctgc cattcggcca acggggatgc agaacccgca actagaaacg ggcgaaattg
      301 aggtggcggt tgagtccttt gccgttttga atgagtcgaa aacgccgccc tttgaaattg
      361 cagatgactc ggacgccacc gaagaactgc ggctaaagta ccgctattta gacctcaggc
      421 gaaaaccgct gcaggagaat atccgcttgc ggcaccgggt ggccaaagcg gtacgggact
      481 atttcgactc ggtcgggttc tacgaaatcg aaacgccggt attaatccgc tccacgccgg
      541 agggagcgcg ggactacatc gtaccgtcgc gggtcaatcg cggcaagttc tatgccctgc
      601 cgcagtcgcc gcagctgctc aagcagattt tgatgatttc cggcttcgac cgctacgtgc
      661 agttggcccg ctgcctgcgc gacgaagacc tgcgggccga ccgccagccg gagcacacgc
      721 aaatcgacct cgaaatggcc tttgtgaccc aggatgacgt tttttccgtc gtggaaggga
      781 tgttcaagca cattttcaaa gtagtccgca acgaggagtt gccgacaccc ttcccgcgca
      841 tccgctacgt tgacgcgatg aaccgctatg gctccgataa gcccgatttg cgcttcggga
      901 tggagttggt ggatctcact gacttagtcc agcagtcacc ctttcgcgtt ttctcggaaa
      961 cggtaaaaaa taagggcgtc gtgaaagggc tggcagtcaa aggccaggcg gcaatgacgc
     1021 gcagccagat tgaccactgg gaggagacag ctcggaagtg gggagccaag ggattgatct
     1081 ggattgttag aaccaaagat gaagttcgtt cgcctttgac gaaattcgtc ccaaaagaag
     1141 aaatagatac gctcgtgaag cgattgagtc tggagattgg cgacctggct ttcctcgtgg
     1201 ccgaccgctg gcacgtcacc tgtaatgtcc tggggcggct gcgcgtggaa ttagcccggc
     1261 agttgggctt gattccgcaa gaccagtgga atttctcttt cgtgatagaa tttccgctct
     1321 tcgaatatca cccgcaagag aagcgctata acgcgatgca taacattgtc acctccccct
     1381 gggccgaaga tattccgaaa ttagatgccg gtttcacctc tcctttgtct cttgaccacc
     1441 ccgaccaccc ttgggggcaa atccgcgcca accagtatga tttagtgctc aacggcaacg
     1501 agatggcctc cggcgggatt cgcaaccacc ggcgcgacat tcaacagaag atattgaacg
     1561 ttttagggat gtcggacgaa cgggcggagc ggatgttcgg gtttcttttg acggctctcg
     1621 aatatggcgc accgccgcac ggcggaattg cgttagggct tgaccggatc gtgacgttgc
     1681 ttaccggctc cgattccatc cgcgacgtaa ttgctttccc gaaaaccgcc gcagcgcagt
     1741 cgcttatgga tggtacgccg gcggaagttg atccggagca gctggcagag ctgggcattc
     1801 gcatcgtcaa ggagtaactt tggaagacac aaccactcga aggattgttc tctccgacat
     1861 tgacccgcgt ctggttttcg gccagcacga tgcctacctg cggatcatcg aaagccaatt
     1921 cggggtgaaa atcatctcgc ggggacagga tctcaccgtg gaaggtgagc cgtccgaggt
     1981 cacgcaagtt gagaaagtaa tttccgacct tattacccgc atcaaagcga accaggaagt
     2041 caatgaacgc tacctcttgt acgccattgc catggtgaaa gaaaatggcg ccggcccggc
     2101 ggatcagttg caactgaacg cgctcatcac gtccctctcc aaggagccgg tccagcccaa
     2161 gacggtggga caaaaggcct acgttcaggc gatgacccag tacgacatcg tcttttccat
     2221 cggcccggcg gggacgggca agacgtacct cgctgtggcg atggcggtgg ccgagttgaa
     2281 gaagaaaatg gtgaaccggg tgattctggt ccgtccggcg gtggaagccg gagagagttt
     2341 gggttttttg cccggcgaca tccgggccaa ggtcgatccc tacctgcggc cggtctacga
     2401 cgcactgcac gaccttttag cgggagaaaa aattcggaag ttaattgaag cggggatcat
     2461 cgaaatcgtc cccctggcct tcatgcgcgg gcggaccctc aacagcgctt tcgtcatcct
     2521 ggacgaagcc cagaacacca ccaacgcgca gatgaagatg ttcctcacgc ggctggggaa
     2581 taactccaag gcggtgatca ccggcgacat cacgcagatt gatctgaatg acaagggggc
     2641 ctccggtctg gtgcagattc aaaagatatt gaccgggatt ccgggcatca agttcgtcta
     2701 cctcaccgaa aaagacgggg tgcgccaccg gctggtgcag gacatcattc tggcctacga
     2761 gcggtacgag aataagaagt cgaagtagaa ggctacgggt ggggcaatga gttcacatcc
     2821 aaagtctcct cgcagaaaaa aggggtccag ccgcatactc gtggccaacc tgcacccgga
     2881 aagaagagtg gtcctggcgc ccttccgtcc cttggtgcgc tcgattctca aaggcgaaaa
     2941 gcgcgcgggc gacatcacca ttgtcttcat taccgacgaa cggatggccg atctcaactt
     3001 ccgcttccgg cgcaaaaacc gcacgaccga tgtcctggcg ttcccgttga acgataaact
     3061 ggccgccaac gggcgcgtcc agggtgagat ttacattgcc ttggaccagg cccgcaggca
     3121 ggcgaaagaa tacggggcca cgttggccga agaactcaag cggctggtgg tgcacgggac
     3181 cttgcacctt ctgggctacg atcatcaccg ccgtgcccag gcccgccgaa tgcgggacaa
     3241 agaagcgcgt taccttttcg cttaacccat gctcatcctc tatctcctcc tttcactttt
     3301 ctttctcttc ttgggctacg cagcaacggc ggccactgcc gaactctacc gtgatccgca
     3361 agaattagaa aaattgacgg ctgatcaaaa ggaaagacgc tttttgaagc gggttttggc
     3421 ccggccccag gagacgctct tcgtcgccag tgctttccgg gcgcttctgt tcctcaccct
     3481 caccgtcctc actttggaaa ttcagcgcct tgccagcgcg ggactgccgg gattttcttg
     3541 ggtttggtac tttgccggat ttctggcgct ctggcttttc tggcttttga ccgtggaagt
     3601 cttccccaaa ttccgtctgc tctccgggca accgagtaaa ctgattcgct acctgtggct
     3661 ggtggggcct ctggtcatcc tgttcaaacc gctttccgca ctcctcttcc cgccgcggga
     3721 acgccgcacc cacgatccgg aattagaaga gaaaaaagag gagatggtgg aacgggcgct
     3781 ggagactctg gcggagacct ccggcgtgga cgagccctta atcgagcagg aagagaagaa
     3841 aatgatcagc cagatcctcc ggctggacac cacagcggtg aaagaaatca tgattccccg
     3901 ggtgaacatc gtctgggtca atcacacggc gtcgttcgat gaggtaaaag agatcgtcaa
     3961 gaccagcggg cactcgcggc tgccggtctg ccgggagacc atcgacgaca tcctgggcat
     4021 cctctacatc aaagatttga tccacgccgc catcatcgag ccgacgccgg actggtcgca
     4081 gtacgtgcgc aagtcgtact ttgtgccgga gtcgaaaaaa atcgacgaac tattagatga
     4141 attgcgctcg cggaagaccc acatggcggt ggtggtggac gagtacggcg gtaccgccgg
     4201 tatcgtcacc ttagaggaca tcctggagga aatcgtcggc gaaattcacg acgagtacga
     4261 cgtgggcgag ccggagattc aaaaagtgga cgaccagacc ttccgggtga ccgggacggt
     4321 caatgtccag catttggccg agacggtcgg cgtcgatctg cccgaggaaa aatttgaaac
     4381 ggtcggcggg ctgatttacg atctggtcgg cagtttgccg gaagagggga aagtcgtgga
     4441 gcatgcgggg ctgaggttta aagttgaaaa agtggtgggg cagaggataa aaaagatttt
     4501 agttgcgcgg acttctcact aaggacgatc aaaaactgac ctttcccctc cccttactga
     4561 cctttccctc cccttgaggg gagggcaggc catacggccc gtatggggag gggtcaaact
     4621 gctatagaaa cggaaaggat ttgtgggtct aaaaaaccgg ccttaaggcc ggttctgtaa
     4681 cgggcttgtc cagaagaggc tctgccgcat ctaaagatac gggcccacct tgttttttcc
     4741 tctccggcat gctaccctct cctctggagg gttagcctaa ttggcaaggc accagacttg
     4801 aaatctggcg accgcaaggt catgggggtt cgagtccctc accctccgtt ctgctgaaat
     4861 tgattgcctc attataaaac aggagttata tttagcatac cggttcgcca cccggaaggg
     4921 ctcgcggttc tccaccaagc gagtgaaagg agcgcttatg ttgcggtacc aaatgaagcg
     4981 ccggggcatg tgcttacgct ggctggcaaa agccaacctt attatgggag gaatattcct
     5041 tgcatcagcc gtaaagccgg cacaggccgg gccgttcgtc gattccttaa ttgtggagtc
     5101 caagactttc ggtcccgcga gccagggagc tattgaaatt ttcgtcagta atgacagtgc
     5161 cgttaccagt atccagttgc cgctggtgat ccattctctt tcgggcggtg cttttgcgag
     5221 aatcgactca gtgaagcagc ttttcacgag ccctttgcca ccaggaaccc gcacgccggt
     5281 gttccgggcg ttcgtcacta ataatagcgg tctagggacc tatgcagtgg actctggcgg
     5341 tccccttctt gtcattcaca ccggcgggca gccgaatgta ccgttttttg tcaggggagt
     5401 tgagacaata gggaatcaag ctccaattct gcggtgttcc ccaggtcgca cggtctactt
     5461 cggggcgtca gtttcgctcc tgccattaga agcgagtgac ccggaaggcg accagctttt
     5521 gttttcgttg gtcggcttca gcaaaatagg tggtggtggg ccacctcaga attcaccagt
     5581 agtggatgtt aataccggtc gctttttctg gcagaccgtc agtaacgcca cagaggttgg
     5641 cacgtgggag acgacccttg gtgtttggga tcctctatca ttttctccgg taacatgtac
     5701 tttcacgatt gaggtaaccg atttgcgcgg cgacgtgaat aatgacgggt tgtggagtga
     5761 cgtggtggat attattgagc tgatcaattt ggtagttttt cagacttcca ttacatcaga
     5821 gaatccgaac agtgcggatg tgaactgcag tggggtggtg gacttagagg atattgtcgc
     5881 gttgatcagt ttcgtggtct tcggggcacc ggcaccgtgc cggggatagt gaggacggta
     5941 tgaataggaa atacctggga tttgcggtac tacttttgct attcagctca agcgcgagcg
     6001 ccgggccgtt tgt
//
LOCUS       DBNGZD010000436         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1736408, whole genome shotgun sequence.
ACCESSION   DBNGZD010000436 DBNGZD010000000
VERSION     DBNGZD010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1736408"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="AC16GM_11370"
     CDS             complement(<1..1506)
                     /locus_tag="AC16GM_11370"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR2924306.1"
                     /translation="MRDLASEWAKHYPDFVKGLEIGKSFLGKPLSGIEVTNSKTGKAS
                     DKPALLLVGNLAGNEPVGSEVCLFAIEQAVASGDTAWENLLKRITLYVIPCLNPDGLE
                     QFLGTSKWSSGLNLRPFDDDRDDAVDEDGPEDLNKDGIITEMLVPDPNGLWRKDSADA
                     RLLVKKKENEPGQYRRYATEGIDNDGDGLINEDGPGGTNLDRNFPAFWDMHYRQPYAG
                     MRQLSEPETRALADFVLAHPNIACVLVLGGAGGQLLRPFAGQGDEGQPVNLPEKDKQI
                     FATIGEAFKKLVPGQEFSQAEGKIAKPGLGTLLDWCYAHEGITSFLYLPWSLPADTAQ
                     KDTTGKGEPGKEMKGKKSQEGEKEPEDKKWLEFSDKRLNKGGFIEWTKFKHPTLGEVE
                     IGGFKPFVKDNPPEAFLRETCVKAVPFIRYLAGVLPQVQIVDVEQKSATGGLVRITAT
                     FADGGFLPPLTVQGTKVMAVPPSLATIELGQGVELVEGEKRVQLDVLEQNGP"
     gene            1770..2162
                     /locus_tag="AC16GM_11375"
     CDS             1770..2162
                     /locus_tag="AC16GM_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924307.1"
                     /translation="MDDPQQKMLSARLTKPELSPREARDGLLASLCLTNRTLLKGSKP
                     QVVADSDEHPLDRMVLALARHVFKSLGADYEIPTLAQLQQAKEILDQKLKLAERLPTL
                     KTQHDAICQLLWSKVPAPPPAAETLTLK"
     gene            complement(2149..2430)
                     /locus_tag="AC16GM_11380"
     CDS             complement(2149..2430)
                     /locus_tag="AC16GM_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924308.1"
                     /translation="MKQAVLTAGQTRGQHGIKISVSYALKERIVAVATRLDRSLADTV
                     RLLLRVGLPVVERLDQDEEKMLLQAVERTRRGRKKKGFWAIMSEKPTSA"
BASE COUNT          506 a          780 c          699 g          555 t
ORIGIN      
        1 cgggccgttc tgttctaaaa cgtccaactg cacccgcttt tcgccttcca ctaattccac
       61 accctgaccc aattcaattg tggccagcga gggcggcacg gccatgacct ttgtgccctg
      121 aacagttaag ggaggcagaa agccgccgtc ggcgaaggta gcggtgattc gtaccagtcc
      181 gcctgtagcg gatttctgct cgacatcgac gatttgcacc tgtggcagaa ccccggctaa
      241 gtagcggatg aagggaactg ctttcacgca ggtttctctt aggaacgcct ccgggggatt
      301 gtctttgacg aacggcttga agccgccgat ttccacttcg cccaaggttg gatgtttgaa
      361 tttcgtccac tctatgaacc cacctttgtt cagtcgcttg tcggaaaact ctaaccactt
      421 tttgtcctct ggctcctttt cgccctcttg cgactttttt cctttcattt ctttccccgg
      481 ctcccctttg ccggtcgtgt ccttctgagc ggtatccgcc ggcaaactcc agggcaggta
      541 gaggaaactg gtgatcccct cgtgtgcgta gcaccagtca agcaatgttc cgagtcccgg
      601 cttggcgatt ttcccctccg cctgactgaa ttcctggcca gggacgagtt ttttgaacgc
      661 ttctccgata gttgcgaaga tttgtttatc tttttcgggc agatttaccg gctgaccctc
      721 atctccctgc ccggcgaagg gccgcaataa ctgtcctcct gctccaccga gaaccagaac
      781 gcaggcgatg ttcggatggg ctaagacaaa atcggccagc gcgcgcgttt ccggctcgga
      841 gagctggcgc atccctgcgt acggctggcg gtagtgcata tcccagaagg cgggaaagtt
      901 gcggtcgaga ttcgtgccgc ccgggccgtc ttcgttgatc aacccgtcgc cgtcgttgtc
      961 gatcccttcg gtggcatacc gccggtactg ccccggctcg ttctctttct ttttcactaa
     1021 gagccgcgca tcggcggaat ctttccgcca gaggccgttg gggtcgggca cgagcatttc
     1081 ggtgatgatg ccatccttat tcagatcctc cggcccgtcc tcatcaacgg cgtcgtcccg
     1141 gtcgtcgtcg aaggggcgga gattcagccc cgaactccat tttgaagtgc cgaggaattg
     1201 ctccaaccca tccggattga gacaggggat gacgtagagc gttattcgct tgagcaaatt
     1261 ctcccacgcc gtgtcacccg aggcgaccgc ttgttcaatt gcgaacaaac agacctcgga
     1321 gccgaccggc tcattccccg ccagattgcc gaccaaaagc agcgccggct tgtcgcttgc
     1381 tttgccggtc ttactgtttg tgacttcaat tccagagagc ggtttcccga gaaaactctt
     1441 cccgatctcc agccccttga cgaagtccgg gtaatgcttg gcccactcgc tggccaaatc
     1501 gcgcatggcg ggggaggcgt gataaaccca cgtcggggat ggttgggcaa ccgagaaagc
     1561 gggtggagag aacaagcaaa tgaaaatggt cagcgcgaac cggcgcatag aagctcctat
     1621 caagtgggaa ccggaaaaga tgcggcggga gttccagatc atttcgctcc gccagtatac
     1681 gctccgggat gcgggctgac aaggcgggga gcaagatcgg ggaggtaaag caggcgaacg
     1741 attcgtccga aaataggaac ggatacgcta tggacgaccc gcaacagaaa atgctctcgg
     1801 cgcggctgac caagccggag ctctcccccc gcgaggcccg ggacggcctc ctggccagtc
     1861 tctgcctgac taaccgcacc ctgctcaaag gcagcaaacc ccaggtggtg gccgactccg
     1921 acgagcaccc gcttgaccgg atggtgctgg ccttggcccg gcacgtcttc aaatcgctgg
     1981 gcgccgatta cgaaattccc actctggcgc agctccagca ggcgaaagaa attctggacc
     2041 aaaaactcaa actggccgaa cgcctgccga ccctgaagac ccagcacgac gcgatctgtc
     2101 agttgttgtg gtcaaaggtc ccggctcccc cgccagccgc cgagacgctc acgctgaagt
     2161 aggtttctca ctcattatcg cccagaaacc tttctttttc ctgccccgcc gggtgcgttc
     2221 caccgcctgt aaaagcattt tttcttcgtc ctgatcgagc cgttccacca ccggcagtcc
     2281 tacccgcaac agcagccgca ccgtatcggc cagggaacgg tccaatcggg tggcaactgc
     2341 aactatccgc tctttgagcg cataagagac ggaaattttg atcccgtgct ggcctcgggt
     2401 ttgcccggcg gtcaaaaccg cctgcttcat ttcccctacg gaacagacct cggatgacga
     2461 aggtgcgcga tcagtgtgcg gggcgaggga ttttccaggc cgtaccgggc tttcaactgc
     2521 tgcaaaacgc gtgaccatgt
//
LOCUS       DBNGZD010000437         2624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_832747, whole genome shotgun sequence.
ACCESSION   DBNGZD010000437 DBNGZD010000000
VERSION     DBNGZD010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_832747"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..925
                     /locus_tag="AC16GM_11385"
     CDS             254..925
                     /locus_tag="AC16GM_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924309.1"
                     /translation="MALCLHLVKVAVESKGMLSNGLKRGFPPERLRSQTEPKVKRDDG
                     GYFIFTLNEGVKVYFEEYYHFLEQLESRAHTEQKNLIHKINHTDPEHLETLSFYRARK
                     VIIDLVLRTIYSFYTDGNNLGVIMSPWCFGTVVLEKAEIYREKIARGEVQDQNIPDYP
                     YYVLKYLDEIYKRTLLELFGFPEKAFSVRWQYTELLKRYSKVLTGVTSSLQSVLLSLK
                     HFGGT"
     gene            complement(1172..1684)
                     /locus_tag="AC16GM_11390"
     CDS             complement(1172..1684)
                     /locus_tag="AC16GM_11390"
                     /inference="COORDINATES: protein motif:HMM:NF040144.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HIR2924310.1"
                     /translation="MRRVSFLFFLLAWMAVSGQSQTLDTARNQSKPGPRRSPTGALLR
                     SVAFPGWGQLYNRKYFKALLLGGTEIGFGISAGVQWHRRDVHKKRFDSATDPGVRAFE
                     ESQFQFYEDNRNLFLWLVAGTIFYSRLDAYVDAHLAQYTEEGLPPLSLDYKPEEWGWG
                     VISLRLRFVW"
     gene            complement(1677..>2624)
                     /locus_tag="AC16GM_11395"
     CDS             complement(1677..>2624)
                     /locus_tag="AC16GM_11395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR2924311.1"
                     /translation="FSSVNGGSCKLIINPAAGFGRTQKLLPRIFAALERQGITYQFVT
                     TTRAGEATGVARQAAFDGFHTVVAVGGDGTVNEVAVGLVDSNAALGVIPTGNGNDFAR
                     SVKIPLDLSPACQHLRTGQTVTIDVGRVNHQFFFNEVGVGLVGDVARLAQMQKRGPGK
                     WRYVIAALKALRTFAPFKVSLKIDQVKLEKKIALLSIGNGTSTGGGFRLTPDASPADG
                     RLDLCVVAAASPWYLLRNLTKAYRGRHTQLPVTTIFRARHIEIASEQPLPTHVDGEVL
                     SPAPTLLSVTASSHRLKVIAPAPVKTPETPQVRQQAAHA"
BASE COUNT          580 a          772 c          735 g          537 t
ORIGIN      
        1 tgcgagaaaa aacattcaac caaaagaatg cttgctcaac gcaattgatc atgcctcctc
       61 cattcccaat ctgtgaactc gtttgcaggc cgggccggtg tcagtatctg tttctattta
      121 agtcgtacga cataggcgta atggcctctt aactctactt ttcaggcgat ccgtaattct
      181 ttttctacca acgggttagt taaaccgctc cccgacgcca tctcgttttg cgccgcttca
      241 ggccgtcttt gtaatggcac tatgtttgca cttggtgaag gttgccgtgg aatcgaaagg
      301 gatgctctcc aatgggctca agcgcgggtt cccgcccgaa cgtctccgct cgcaaaccga
      361 gccgaaagta aaaagagatg acggcggcta cttcatcttc accctcaatg agggagtgaa
      421 ggtttacttc gaagagtact atcacttttt agagcagctc gaatcccggg cgcataccga
      481 gcagaaaaat ctgattcaca aaattaacca cactgatccc gagcacctgg agaccctctc
      541 tttctatcgt gccaggaagg tgatcatcga tttagtactg cggaccatct actcgttcta
      601 caccgacggg aataacctgg gcgtgattat gtccccctgg tgcttcggca cggtggtgct
      661 ggagaaagcc gaaatctacc gggagaagat cgcccgcggc gaggtgcagg accagaacat
      721 cccggactac ccctactatg tgctgaagta cttggacgaa atctacaagc ggacgctctt
      781 ggagttattc ggcttcccgg agaaggcgtt ttcggtccgc tggcagtaca cggaactgct
      841 caagcgctac tcgaaggtct tgaccggcgt gaccagttcg ctccagagcg ttttgctttc
      901 gctgaagcac ttcggcggga cgtaagaatc cgctggaggg tgagcgagga cgctcgccct
      961 acgattggga aagagtttcg gacgctgaac ccccgaccaa tcggggaggt cccgccaaaa
     1021 gtgcgggaca gggcgggtgc cgcaaccgcc cttcaatttt taggtctcat cgaatacgct
     1081 gttttaaccg caaaggcgcc tctcgcactc gatggcgtaa atatcaggag ggggttgctt
     1141 gcccatcagg agtgaataca gcgcagaaca gctaccacac aaatctcagc cgcaacgaaa
     1201 tcactcccca cccccactct tccggcttgt agtccagcga caggggcggc aggccttcct
     1261 cggtatactg ggcaaggtgc gcatcgacgt aggcgtccaa cctcgagtag aaaatcgtcc
     1321 cggccacgag ccacaggaag aggttgcggt tatcctcata gaactgaaat tggctttctt
     1381 caaacgctct cacccccgga tcggtggcgg aatcgaagcg ctttttgtgc acgtcgcgcc
     1441 ggtgccactg cacacccgct gaaataccaa aaccgatctc ggtcccgccc aggagaagcg
     1501 ctttgaagta cttccggtta tagagttgcc cccagccggg aaaggccacc gagcgcagaa
     1561 gcgcaccggt aggcgagcgg cgcggccccg gctttgattg attccttgct gtatccagag
     1621 tctgggactg gccggaaacc gccatccagg ccagaagaaa aaaaagaaaa cttaccctac
     1681 gcatgcgctg cctgttgccg cacctgcgga gtctctggag ttttgaccgg ggccggcgca
     1741 atcactttca gccggtggga cgaggcggtt accgacagaa gtgtcggcgc cgggctcaag
     1801 acctcgccgt cgacatgggt cggcagcggc tgctccgagg caatttcgat atgccgggcg
     1861 cggaagatgg tggtcacggg cagttgcgtg tgccggccgc gataggcttt cgtcaggttg
     1921 cgcaagaggt accagggaga cgccgcggcc accacgcaga gatctaaccg tccgtcggca
     1981 ggggaagcat ccggtgtcag gcgaaagccg ccgccggtag aggtcccgtt tccgatactg
     2041 agcaaggcga ttttcttttc cagtttcacc tggtcaattt tcagcgagac tttgaacgga
     2101 gcaaaagtcc ggagcgcctt cagcgcggca atgacgtaac gccatttccc cggcccccgc
     2161 ttctgcatct gagccagccg ggccacgtcg cccaccagcc ccacaccgac ttcgttgaag
     2221 aagaactggt ggttcacccg gcccacatca atcgtcaccg tctggccggt gcgcaaatgc
     2281 tggcatgctg gtgaaaggtc caaggggatt ttaacgctgc gcgcaaagtc gtttccattg
     2341 ccggtgggga tgacacccag cgcggcattg ctatcgacca gaccaacagc gacttcattc
     2401 accgtcccgt ctccccccac ggccaccacg gtgtgaaaac cgtcgaaggc ggcctggcgg
     2461 gccacgccgg tcgcttcccc ggcccgggta gtagtgacga actggtacgt aatcccttgc
     2521 cgttccagag ccgcaaagat gcgcggcaga agtttctgcg tccggccaaa accggccgca
     2581 ggattaatga tgagtttgca agagccgccg ttcacgctgc taaa
//
LOCUS       DBNGZD010000438        21458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1802980, whole genome shotgun sequence.
ACCESSION   DBNGZD010000438 DBNGZD010000000
VERSION     DBNGZD010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 21458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21458
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1802980"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..718)
                     /locus_tag="AC16GM_11400"
     CDS             complement(338..718)
                     /locus_tag="AC16GM_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924312.1"
                     /translation="MSLKTIKTVFLISAVYDILLGIIFGLFFKAVYTKFGIEIPTNAG
                     YLQLPALYIFIFGLGFYFVYKNPQQNRAIIPLGILMKLAFCLVAFGHLVFDTIPSIYL
                     PFAVIDIVFALLFIAANKAVKKMG"
     gene            complement(699..2069)
                     /locus_tag="AC16GM_11405"
     CDS             complement(699..2069)
                     /locus_tag="AC16GM_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR2924313.1"
                     /translation="MKIGLIAMSGVRAHNEELTQLGLTLPGFVERNKIIASLPSLSLL
                     TLAALTPKKFAVEYHEIADLKKESTLPDDYDLVAISSFTAQMYEAYEVADRYRRKNIP
                     VVLGGLHVSSLPEEAKEHCTSVVVGEGEPLWPQVTADFEKGSLKPYYVQTPPGSFDLK
                     DAPIPRYDLLDPDKYNRLTIQTSRGCPHKCDFCASSILLTSRYKLKPVPQVIAELHAI
                     KKVWDKPFIEFADDNSFVNYKHYKELLRALARENIRWFTEADVAVAKDNELLGLMRDS
                     GCQQILIGLESPRKVSLDGIELNANWKMKQQEKYKEAIAKIQSYGITVNGCFITGLDG
                     DTPEVFEEIFNFVRDSGLYEVQITFLTAFPGTPLYQRLKQEGRIIRDRAWELCTLFDI
                     NFAPKNLTIEQLQNGFLWLAKTLYSEEVTKARRRKFKQMLKTSPNFRRRFKVTREEET
                     HVTEDN"
     gene            complement(2066..2449)
                     /locus_tag="AC16GM_11410"
     CDS             complement(2066..2449)
                     /locus_tag="AC16GM_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924314.1"
                     /translation="MIKINFILAWLWIVVGFVTGFLLGLNFHREEWLGGYTSLKRRLY
                     RLGHISFFGLAILNLLFYFTTQQILPPNRSIDVASWGFVVGAVAMPACCFIMAHKPRL
                     RAIFLIPVLSLTLAGVLTLWEVIGR"
     gene            complement(2474..3790)
                     /locus_tag="AC16GM_11415"
     CDS             complement(2474..3790)
                     /locus_tag="AC16GM_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR2924315.1"
                     /translation="MSWLDDYRRKIGSPEEAVAFVKSRERIYISGNAATPFVLLDALA
                     ARKDDLHDVEVIHVLLLGKGREDPLSRPGMEGHFRHNSLFVGPSDRAAVQTGRADYIP
                     VFLSEIPRLLRTQLPIDVALIQTSPPDEHGFLSLGVECLSTRAALESARVVVAQVNEK
                     MPRTLGDSFVHFSKLAKIVEVAEDLPELLPEPPGAIEEKIGRFVGELVEDGATLQAGI
                     GGIPDAVLQQLRDRKDLGVHTEMVSDGLMELMESGVITGAKKTLHPGKVVATFLLGSR
                     RLYDFAHNNPAFEFHPADYTNDPFLIAQNDKMVAVNSAIEVDLTGQVCSDSIGYQIYS
                     GFGGQLDFVRGAARSKGGKAVIALPSTAKGQSFSRIVPALREGAGVVTTRGDVHYIVT
                     EYGVAYLHGKNLRERLRALIEIAHPKFREDLERSHNQRRVREMSPV"
     gene            complement(3787..4905)
                     /locus_tag="AC16GM_11420"
     CDS             complement(3787..4905)
                     /locus_tag="AC16GM_11420"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR2924316.1"
                     /translation="MIVGVPRERPPGRGMEERRVGLSPAGVAELVKAGARVVVEREAG
                     TGAHFADATYEKAGAQIVLSHEEAFQRADIICKVGRVADDELPLLHEGQILTGFLHLI
                     TAPKSTLQTLLDKKITATGYELIEEADGRLPVLSPTSEIAGKMVPQLAGRLLERGRGT
                     LLGGIPGIPPADVVILGGGTLGYHAARALRGIGAMVYLLEKHPRRLEELDKYFAGSLV
                     TAVANRVNIEKFVPFADVLIGAVLEPGARAPILVTREMVRTMPRGAVILDFSIDNGGC
                     IETSRPTPSEEYVYQEEGVWHYCMPNASSLVARTASHALTNAALPYLKSLVERGVRES
                     LRGMTDLAKGVSAHAGFVTQEHLAREYAFEYRPLERLL"
     gene            complement(5161..6567)
                     /locus_tag="AC16GM_11425"
     CDS             complement(5161..6567)
                     /locus_tag="AC16GM_11425"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR2924317.1"
                     /translation="MAIERETEILDALRKVHDPDLGRDIVSLGFVKNLRVADGNVAFT
                     VELTTPACPVRDALKEEAQRVVLALPGFKKVEVTMTAQVRTTPSEKKEKMVPAVKNIV
                     PVASGKGGVGKSTVSANLAVALAQLGARVGLMDADIYGPSIPTIMGVKQPPTVLSGNR
                     LIPTVEHGVKIISIGFFIRPGDAVIWRGPMLHKTVEQFLANVEWGELDYLIVDLPPGT
                     GDVQLSLCQTIPLTGAAVVATPQDVALQVAEKAIIMFGKLNTPVLGLIENMSGFVCPH
                     CGAREDIFGAGGALAYSLAHDIPFLGEIPLATQIRETSDSGLPIVIAAPDSPAARAFA
                     QAAQNLAAQVSIRNLGGVPPKAGEDRPTPAEISQPKREQVSILWKDGHASLYPARDLR
                     LACLCALCVDEVSRKPLLREEKVPQNVHPNIIHPVGRYALRFEWSDGHSTGIYTFDYL
                     RSLCPCEKCRAKAPPKAG"
     gene            6947..7702
                     /locus_tag="AC16GM_11430"
     CDS             6947..7702
                     /locus_tag="AC16GM_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="actin-binding WH2 domain-containing protein"
                     /protein_id="HIR2924318.1"
                     /translation="MRNGERESVSGRESLLGLLLKDRERFFSEVADNVGIKEKLKFLL
                     TTLIGLSAFYGATVGLYQGGWQILSAALKIPFLFLVTLLISFPLFYIVQLLLGSKLSL
                     LQVLTLILSALALTSLLLAAAVPISLFFLLTGDNYYFLHLLHIAVFAFAGLFGMFTLH
                     AGLTVLCESRGVYPRLGIGILRIWVIVFAFVGIQLAWHLRPFVGKRNEPFAIRKQYEG
                     NFYSAVIYAAGQLFKGDTLTTPTFHPPSENPAR"
     gene            7722..7934
                     /locus_tag="AC16GM_11435"
     CDS             7722..7934
                     /locus_tag="AC16GM_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2924319.1"
                     /translation="MTDLDLMTVEEVAQYLRLKPQTIYKWAQEKKIPAVKLGKEWRFR
                     KRILDEWLDGQISHKQEERDSGQHES"
     gene            complement(7964..8494)
                     /locus_tag="AC16GM_11440"
     CDS             complement(7964..8494)
                     /locus_tag="AC16GM_11440"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR2924320.1"
                     /translation="MKKRSKATEKLYVTSVATRMGTVHLASTPRGVARIALPGENHKA
                     FFSWLVKTFPKAEISTQGRVNEKPARQLREYFEGKRKKFNFRLDLRSTPFQSRVLRKL
                     RAIPYGKTASYGEIAKKIGRSKAFRAVGLACGSNPLPVVIPCHRVIGANGSLTGFGGG
                     LKLKAAMLDLEKTRKK"
     gene            complement(8519..9445)
                     /locus_tag="AC16GM_11445"
     CDS             complement(8519..9445)
                     /locus_tag="AC16GM_11445"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR2924321.1"
                     /translation="MSSITLRPVRPYRFDLILHHWGHNPHEILDVVKNGAFHRVIELE
                     STPVLYRLINSGTNQKPQLSLRFLNGRLSRTQLEKATRIVSRTFSLHVDLKKFYRAVA
                     GDEVLSRIGKTFRGLKPKLIGSTLFENLAIAIMGQQVNLTFATTLKRRLVEAFGPRVV
                     YRGKIFYGFPAAGRLATVDPEKLRKMQFSRQKSRYILDLARQVASGEIDLESYRYLEP
                     DRAVEQLMAIRGIGRWTAEYCLMRGIGYLDALPAGDTGLQNAVTRFYRFKKRATEDQV
                     RKLGERWKPYRSLATFYLWYALSKRLPLIPKS"
     gene            complement(9464..10474)
                     /gene="tsaD"
                     /locus_tag="AC16GM_11450"
     CDS             complement(9464..10474)
                     /gene="tsaD"
                     /locus_tag="AC16GM_11450"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR2924322.1"
                     /translation="MLLLGIETSCDETAAGVLKDRRTLLSNVILSQVVHQPYGGVVPE
                     LASREHMRNLVPIVDQALAEAKTPLEKIEGLAVTFGPGLVGALLVGISFAKAVAVSRR
                     LPIIGVNHLEGHIFSNALEHPELEPPFVCLVVSGGHTLLVLVKNWGVYRLLGQTRDDA
                     AGEAFDKVAKLLGAGYPGGQKIDDLAQTGNPARITFPRAYLEKEAYDFSFSGLKTAVA
                     LYVQKLSERERENLLPHLCAGFQEAVVDVLVAKTVRAAEESNVSAIAVAGGVARNRRL
                     RQRLGDEASRRGMKFFVPVPQFCTDNAAMIAAAGAFHLSRGERSDLSLNAAPYVPLNG
                     VA"
     gene            complement(10474..11391)
                     /locus_tag="AC16GM_11455"
     CDS             complement(10474..11391)
                     /locus_tag="AC16GM_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR2924323.1"
                     /translation="MVKPLWENLPVKVAALLLAVILWFHVSTERRVEHSLAVPLTYTG
                     LDSSLVFLKLPPPAVEVRVRGTGKAVLKAIWQKKAVPVKLDLLGPGNYDRLVRQADLR
                     QALDPELEILELIQPKSLTFSLDRVEEKTVKIESRVTVEPALGYFWNGELTFEPVLAT
                     VRGPQQALAKIEKVFTDTAHLRGTQTPLVATLRLLAPDSFNVSLVTPTVIVRAPVEKV
                     SSLVLDSIPVKTLISALQRVAWIRPNLLRLTLEGPESALRKIDRKTLKAVVNCSRLKA
                     GRHWLVPQVSVPPPVVVDAPLDSVEVRIE"
     gene            11457..12587
                     /locus_tag="AC16GM_11460"
     CDS             11457..12587
                     /locus_tag="AC16GM_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2924324.1"
                     /translation="MAQVRFDRVTKIFDSQQPALTNFFLEVKDGEFVVLVGPSGCGKT
                     TALRLLAGLEEPTAGDLFIGERRVNTIPPKDRDVAMVFQNYALYPHMTVYENLAFPLK
                     VRHIVKNEIDRRVRETAELLGLAPLLSRKPKALSGDERQRVAVGRALVRQPQVFLFDE
                     PLSNLDAALRSQLRGELLHLKTKLGATALYVTHDQVEALTLGDRVAVLKAGELQQVGS
                     PGELYRRPQNLFVATFIGTPPMNLIRARVFRQDGLFCHEVFRWRVPPPQAEILEAYVG
                     REIILGLRPDILRVVATDADASGMERFPISIDFMEPIGGEMLLHGTAAGTPVIITERR
                     VMGERKVSPGEKITCAFEWRQVHLFDPASGQRISFPTLTVPP"
     gene            complement(12571..13506)
                     /locus_tag="AC16GM_11465"
     CDS             complement(12571..13506)
                     /locus_tag="AC16GM_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924325.1"
                     /translation="MVVPVMAVLDFWRRDRFHWRSSLLSIFPFTLIAVASFLYRLPAL
                     LAVDSTAAYVPVIGLHILKNYLFLFTGSLVDLDFRHLLEIWNAFRDSHNFSQLLTQIT
                     RMGVIPAIIVVMFLYILLWWRGGKAVRLMLLWAILFFLPGSLLKGTGERILYLPLFGL
                     VTAWVTFLLPLLRKFWSTARTQRLAAGTVLALFLLWVGYNVFRLHTRVGCWEKAGAIS
                     ENVIQTIDRMQSNWPPGTTVLALDILDNYEGAWVFRMGFEQLPYLFWPTKHFSFAKAD
                     SNSLSQLIRENKNFGRPVRVLRYTNETVVEVTAAR"
     gene            complement(14128..14862)
                     /locus_tag="AC16GM_11470"
     CDS             complement(14128..14862)
                     /locus_tag="AC16GM_11470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2924326.1"
                     /translation="MTTPLTSNRTLTQLADFVGSENLFLQKTPPAVCPRAAAEVKSIV
                     AFACGRKIPLAIQGNGAHLPVSDPNRVVVSLERLNRVVELASDDLYVTLEAGFPLGVL
                     NQTLSPHKVAYPLSQAGYNGTVGGSVAMNLSATLEDDTIQTKTYLMALEAVTGYGELV
                     KLGAKTYKSVAGYDVARFLCGSWGRLAVITKVTLRLIPLRLQKQYEKARLIDPIWPWG
                     QAKRDPALAALENQVKSQVDPTGILP"
     gene            complement(14859..15998)
                     /gene="hemW"
                     /locus_tag="AC16GM_11475"
     CDS             complement(14859..15998)
                     /gene="hemW"
                     /locus_tag="AC16GM_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR2924327.1"
                     /translation="MATLGIYCHLPFCTVKCPYCDFYSIVFKGKGEEEFVSALCREIE
                     MTATTIGVAGCPVDTIYFGGGTPSILAIESLQKILTTLRLFFQIDPQVEITLEANPGT
                     VTEEKFGAFRSSGFNRISMGYQSIFDHHLKILGRDHTAAEAERACLWARRAGFKNISL
                     DFIYALSDQTLAEWEQTLARAIALSPEHISAYCLTIETRTPYALYFQQGRLHLPDEEI
                     SRQMFAAARILLAEAGYEHYETSNWAKPGFRSRHNEKYWTDREYLGFGPSAHSFLRNQ
                     RWANVRDLKVYTARIVEGKLPRDFIETLSPVEERTEAILLGLRRAEGIDLENYALRFG
                     TRLELDKQKEIERFIRLGYLKRTDGHLQLQAEGWFVADGIMAALA"
     gene            complement(16064..17095)
                     /locus_tag="AC16GM_11480"
     CDS             complement(16064..17095)
                     /locus_tag="AC16GM_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924328.1"
                     /translation="MGKLPPIRIDKNVNEAPHAVILGAGASRASFPQGDKYGRKLPLM
                     TDFVETVGIGEFLSEHGVEHPDRNFEEIYDRLYRQTDRRQILQQLETLVYEYFAQMMI
                     PDKPTLYDELILCLRAKDIIASFNWDPLLIQAYRRNSHIKGLPKIVFLHGNVGVGACD
                     NDKVKGYADRRCSKCDKPFTKSPLLFPIRDKNYKERPFIANEWLELEAHLKYAFIVTI
                     FGYSAPSADINAKSLMKLAWDKNEWRELSEMELIDIKGRNELSENWKEFITREHYGTF
                     TEPRKSLIFLYPRRSCDAFAWAILQSDPWYDKPLPRFSSLHELQEWVQPLVLEELRYE
                     ERDEPIAKF"
     gene            complement(17145..17564)
                     /locus_tag="AC16GM_11485"
     CDS             complement(17145..17564)
                     /locus_tag="AC16GM_11485"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2924329.1"
                     /translation="MTVTRTIATGIKRAYAAWADPALLSRWFTTTARRDFQVGGRYEN
                     SDGDSGEFLKIVPNRLIRFTWENKQPQPESRVNVEFSQQGENCTAVRLTHEKLKTKKD
                     ADKLKQGWSWAMDSLKSFLETGIAISYSDWLKQGKHG"
     gene            complement(17594..18469)
                     /locus_tag="AC16GM_11490"
     CDS             complement(17594..18469)
                     /locus_tag="AC16GM_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924330.1"
                     /translation="MPKNKKKNYPGGAEPTIHPASPSKFKRRLTATIILTMVIVGSIF
                     LFTARRNRVAKPSKETTGPLATDKNPEAVTLPGQPDQKPKLNPAPQLMNLEVNQAVMV
                     TVELDFGGKPPAIAEALRGIERISEPFDGVGRTFAVIDAYGEPTAEGKLHLSMHVSTE
                     KPGRGSLVFRRTGEILWQCEMRPSPNPNAAFTGKNLSIFVDDGKGKSLTVVGANNPSS
                     ILTANVRELGRPVASIWPDGAEREVTFIYSACGCPVKVMVKRVGDRTVRTKELPVIFP
                     DDPAAVTTIEQLMRW"
     gene            complement(18609..19067)
                     /locus_tag="AC16GM_11495"
     CDS             complement(18609..19067)
                     /locus_tag="AC16GM_11495"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2924331.1"
                     /translation="MTEVERIADQLNRVFEGDAWHGPSVREVLKDVSAEKAAKKPIPN
                     AHSIWELVLHIAAWENAVRRRIEGDNVDLTDEEDYPPIRDTSESAWQKAMKMLENGHR
                     ALRKVVSGLTDSRLEELIRGRNYSVYYLLHGIIQHDIYHVGQIALLKKAG"
     gene            complement(19086..19511)
                     /locus_tag="AC16GM_11500"
     CDS             complement(19086..19511)
                     /locus_tag="AC16GM_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR2924332.1"
                     /translation="MKRKASAVWKGGLKDGKGTVSTESGVLSNTQYSFGGRFESGKGT
                     NPEELVAAAHAGCFSMALSAELGKANLTPQSINTAATLTLEMLEGGPTITAIHLETTV
                     RIPGANKAAFEKAANDAKAGCPISRLLNTKITMDAKLEA"
     gene            19814..21361
                     /locus_tag="AC16GM_11505"
     CDS             19814..21361
                     /locus_tag="AC16GM_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HIR2924333.1"
                     /translation="MIFRRKKSLIIRLLTWLLVSGYWLSFFGRAAATPLEDIPLDSWV
                     YPALEELYSRGYSYEFFLSDKPYSRGEVARCLKKISASNPHLLENDFLILRLRYEFAD
                     ELAALENPPETDKPLEARGGAFVRGNGITSGKARPIQRGAVGVFLGLEAEDKFVTRAR
                     MTLDTRTQDDPAIRGRRWHNSVTLFFDDAYAKVHTRYLDLFWGRQHLQWGVGSDALLL
                     SNHFPPLEQVKLKANYKTLRYQFLASRLDPIFIDTLGGNVPRFFSAHRLSWKPWPWLE
                     AGVSESIVYGGPGRSFELFYLNPLLPFYAEQYNNFVDDNPFVGVDFLFSRIQHLVFFG
                     ELMIDDFQIDFKSEPQQIGFRLGGWISDFISKGRTFGQLEYTRVNTFVYGQNKPWNIY
                     TRFGAGMGSEIGPDADRWRGSFLWRAHRWVDLVLSGEYRRRGENRITTPQNVAVPYEK
                     FPSGVVEKTALASFTGRLQYQANVFADLTCGGTFLRNGDNRRGNNRDDFFVRGTFTIT
                     FWKTKQY"
BASE COUNT         4671 a         5862 c         6042 g         4883 t
ORIGIN      
        1 cctgcccctc taccttgcgg aatatgtatg gcgctacaat caccggcgtt tatctttccg
       61 acaacagacc caaacgctca tgggactgct gagactcaca taggtggctg gaatgggact
      121 ttacccaatt gaatagtgaa cgcagattcg aaaatccgtg tttaccttat cccaatattt
      181 tgacttttca agttcttcaa gattggcttg aaaaatctcc aaaacttccc tcagattttt
      241 gatccaaccc ttgagatccc cgattatgtc ttcaaatgac tgatgcgagt aaaccgttga
      301 tccggcaaac agtgatcgtc ccattattcc atctccctca tcccatcttc ttcaccgctt
      361 tattcgctgc aatgaaaagg agcgcaaaga cgatgtctat caccgcaaac gggaggtaga
      421 tggagggaat ggtgtcgaag accagatggc cgaaggcgac gaggcagaag gcgagcttca
      481 tcaaaatccc caatgggatg atcgcccggt tctgctgcgg gtttttgtag acgaagtaga
      541 agcccaagcc gaagatgaaa atgtagaggg cggggagctg gaggtagccg gcgttagtcg
      601 ggatttctat tccaaacttg gtgtaaactg ctttgaaaaa cagcccgaag atgatgccga
      661 gaagtatgtc gtacacggcg gagatcagaa agacggtttt aattgtcttc agtgacatgg
      721 gtttcctcct ccctggttac cttgaatctc cttctgaaat tcggggacgt tttgagcatc
      781 tgtttgaatt tccggcggcg cgctttagtc acctcctccg aatatagagt tttcgccagc
      841 cacaggaaac cattctgcag ttgctctatg gtcaagttct tcggcgcgaa gttgatgtca
      901 aagagggtgc agagctccca ggcccggtcg cggataatgc ggccttcctg ttttaacctc
      961 tggtataaag gcgtgcccgg gaaggcggtc aaaaaggtga tctgcacctc gtataaaccg
     1021 gagtcgcgga cgaagttaaa aatctcctca aaaacttccg gggtgtcgcc gtccaagccg
     1081 gtgatgaaac agccgttgac cgtaatccca tacgattgaa tcttggcaat cgcctctttg
     1141 tatttctcct gctgtttcat tttccagttg gcgttcaatt cgataccgtc cagactgact
     1201 ttgcgcggac tttccaaccc gatcaaaatc tgctgacagc cggaatcgcg catcaaaccc
     1261 agaagttcat tgtctttcgc caccgccacg tcggcttcgg tgaaccagcg gatattctct
     1321 ctggccagcg cacggaggag ttccttgtag tgcttgtaat tgacaaaact attgtcgtcg
     1381 gcgaattcga tgaagggctt gtcccagacc ttcttgatgg cgtggagttc ggcaattacc
     1441 tgcggcaccg gcttgagttt gtaccgggag gtcaaaagaa tcgaactggc gcagaagtca
     1501 cacttgtgcg ggcagccccg gctggtctgg atggtgagcc ggttgtattt gtccggatcg
     1561 agcagatcgt accggggaat cggggcgtct ttcaggtcga agctccccgg cggagtttgc
     1621 acgtagtaag gtttcagaga acctttctca aaatccgccg taacctgcgg ccagagcggc
     1681 tcgccctcgc ccacgacgac gctggtgcag tgttctttcg cctcttcggg cagcgaagag
     1741 acgtgcaagc cgcccagcac caccgggatg tttttgcggc gataccggtc ggccacctcg
     1801 taggcctcgt acatctgcgc agtgaaggaa ctgatggcga ccaagtcgta gtcgtccggc
     1861 agtgtggact cttttttcag gtcggcaatt tcgtggtact cgactgcgaa ctttttcggc
     1921 gtcagcgcgg ccaaggtcaa aagcgataaa ctggggaggg aggcgatgat tttattgcgc
     1981 tcaacaaagc cggggagggt caaaccgagt tgagtcaact cctcattgtg cgcccggacg
     2041 ccgctcatgg caatcaagcc gattttcatc tgccgatcac ctcccaaagg gtcaaaactc
     2101 cggccagggt cagactcaag accggaatga gaaagattgc ccgcagtctt ggtttatgcg
     2161 ccatgataaa acaacaagca ggcatcgcca ccgctccaac gacaaagccc cacgaggcaa
     2221 cgtcaatcga acggttgggc gggaggatct gctgggtggt gaagtagaaa agtagattca
     2281 ggattgccag accgaagaaa gaaatgtggc ccaaacggta gaggcggcgc tttagactcg
     2341 tgtacccgcc cagccactct tcccggtgaa agttcaagcc caatagaaaa ccggtgacga
     2401 aaccgacgac aatccagagc caggccagga tgaagttgat tttgatcatg gaaaaattca
     2461 gagtcccccg ttttcagacc gggctcattt cccggacgcg tctttggttg tgggagcgct
     2521 ccaggtcttc gcgaaacttc gggtgcgcga tctcgattaa cgcccgcaag cgttcacgca
     2581 ggttcttgcc gtgcagatag gccacaccgt attcggtgac gatgtagtgc acgtcgccgc
     2641 gcgtcgtcac cacacccgct ccttcgcgca gggccgggac gatgcgggag aaactctgcc
     2701 ctttggctgt cgacggtaaa gcgatgaccg ccttgccgcc tttcgaccgg gccgcaccgc
     2761 gcacgaaatc gagttgtccg ccgaagcccg agtaaatctg atacccgatg gagtcggaac
     2821 agacctggcc ggtcaggtcg acttcgatgg cggagttgac cgccaccatt ttgtcgttct
     2881 gggcgatgag gaaaggatcg ttggtgtagt cggcggggtg gaactcaaag gcggggttgt
     2941 tgtgagcgaa gtcgtagagc cgacgtgaac ccaagagaaa ggtcgccacc accttccccg
     3001 ggtggagcgt ctttttcgcc ccggtgatga cgcctgattc catcaactcc atcaggccgt
     3061 cgctcaccat ttcggtgtgc acgcccaggt ctttccggtc gcgcaattgc tgcaggacgg
     3121 cgtccggaat tccgccgatg cccgcctgca gggtcgcccc gtcttccact aattcgccga
     3181 cgaagcgccc gattttttct tcgatagcac cggggggttc cggcaaaagc tcgggcaggt
     3241 cttcggcgac ctccacgatt ttggcgagtt tcgagaagtg cacgaacgaa tcgcccaggg
     3301 tacgcggcat tttttcgttc acctgcgcga cgacgaccct ggccgattca agggcggccc
     3361 gcgtggagag gcactcgacg cctaaactga gaaagccgtg ctcatccggc ggcgaagttt
     3421 gaatcagcgc cacgtctatc ggcaactgcg ttcgcagaag gcgcgggatt tcggagagga
     3481 agaccgggat gtaatcggcc cggccggtct ggaccgccgc ccggtccgaa gggccaacga
     3541 agagcgagtt gtgccggaaa tggccctcca tccccgggcg ggagagcggg tcctcgcggc
     3601 ctttgcccag aagcagaacg tggatcacct ccacgtcatg cagatcgtcc ttgcgtgccg
     3661 ccagagcgtc caagaggacg aacggcgtgg cggcgttgcc gctgatgtag atgcgctccc
     3721 ggctcttgac aaacgcgacc gcctcttcgg gtgagccgat tttgcgccgg tagtcgtcca
     3781 gccagctcat aggagccgct ccagcggtcg gtactcaaaa gcgtactcgc gggccaggtg
     3841 ctcctgcgtg acgaagccgg cgtgggcgct gacgcctttg gccagatcgg tcatcccccg
     3901 cagcgattcg cgcacgcccc gttcgacgag acttttcaag tacggcagcg ccgcgttggt
     3961 gagggcgtgg gaggcggtgc gcgccaccag actgctggcg ttgggcatgc aatagtgcca
     4021 gacaccctct tcctggtaga cgtattcttc ggagggggtg ggccgggagg tttcgatgca
     4081 gccgccgttg tcaatcgaaa aatcaagaat caccgcgccg cgcggcatgg tgcggaccat
     4141 ttcgcgtgta accagtatcg gtgcacgcgc ccccggctcc aggaccgctc cgatcagaac
     4201 gtcggcgaag gggacgaatt tttcgatatt gacccggttc gccacggcgg tcaccagact
     4261 gccggcgaaa tatttatcca gttcctccag ccgacgcgga tgtttttcca ataagtatac
     4321 catggcgcct attccgcgca gagcccgggc ggcgtggtag ccgagtgtgc cgccgcccag
     4381 gatgaccaca tccgccgggg gaatgccggg tatcccgccc aagagcgtcc cgcgcccgcg
     4441 ctccagcagg cggcccgcca gttgcggcac cattttgccc gcgatttcgc ttgtgggact
     4501 gagcaccggc aggcggccgt ccgcttcctc aatcagttcg tagcccgtgg cggtgatttt
     4561 tttgtccaga agcgtctgga gcgtactttt gggtgcggtg atcaaatgca gaaagccggt
     4621 gaggatctga ccttcgtgca ggagcgggag ttcgtcgtca gccacgcggc cgactttgca
     4681 gatgatgtcc gcgcgctgga atgcttcttc gtgcgacaga acgatctggg cgccggcctt
     4741 ttcgtaggtg gcatcggcga aatgcgcacc ggtcccggct tcacgctcga ccactacccg
     4801 ggcgccggcc ttgactaatt ccgccacgcc ggcaggcgaa agcccgaccc ggcgctcttc
     4861 catcccccgc cccggcggtc gctcccgcgg gactccgaca atcataggat gcctcgccgg
     4921 ctcccggcgt caacgtggtt ggcacacaat tcaagagtag agaagtcttt tggcatactc
     4981 tttcaatata ccaagcgctc tgagcgaccc caaatcaggg gcaaattgag agcacaccag
     5041 tagggataag aacgcattcc tctttttgta ggcgaagccg gaagcggctc gtcccgggga
     5101 cgaagtgaaa agtcttttcg ccttgacggc gaggtgagtg ctctattttg ggaaagaagt
     5161 tcaacccgct tttggcggag cctttgcccg gcacttttcg caaggacaga gcgagcgcag
     5221 gtagtcgaag gtgtaaatgc cggtggaatg cccgtcgctc cactcaaaac gcagggcgta
     5281 gcgccccacc gggtgaatga tgttcggatg aacgttttgg ggaacttttt cttcccgcaa
     5341 aagaggtttg cggctcactt catccacgca cagggcgcag agacaggcca ggcgcaggtc
     5401 gcgggccggg tacaagctcg cgtgtccatc tttccagagg atcgagactt gctctcgttt
     5461 cggctgggag atttcagccg gagttggacg gtcttctccc gcctttggcg ggacaccacc
     5521 cagattccgg atacttacct gcgcggccaa gttctgggcc gcctgagcga aggccctggc
     5581 ggcaggcgag tctggggcgg cgataactat cggcagtccg gagtcggaag tctctctgat
     5641 ctgagtggct aaaggaattt cgcccaggaa cggaatgtca tgcgctaaac tgtacgccag
     5701 cgccccgccg gcgccgaaaa tatcctcccg cgccccacag tgcgggcaga caaagccgct
     5761 catattttca atcaaaccca gaaccggcgt gttcagtttg ccgaacatga tgatggcctt
     5821 ttccgctacc tgcagggcca cgtcctgcgg cgtggccacc accgccgcgc cggtcagggg
     5881 tattgtctgg cagagcgaga gctggacgtc gcccgtgcca ggcgggagat caacgatcag
     5941 gtagtcgagt tcaccccatt caacgttggc aaggaactgt tcgaccgtct tgtgcagcat
     6001 aggaccgcgc cagatgacgg catcgccggg ccggatgaag aagccgatgg agatgatctt
     6061 gacgccgtgc tcaaccgttg gaatgaggcg gttgccgctc aaaactgtcg gcggctgttt
     6121 cactcccatg atggttggaa tgctggggcc gtagatgtcg gcatccatca accccacccg
     6181 ggcgcccagt tgcgccaggg ccaccgccag gttggcgctg acggtggact ttcccacacc
     6241 gcctttgccc gaggcgaccg gcacaatgtt tttcaccgcc ggaaccattt tctctttctt
     6301 ttcggagggg gtggtccgca cctgggcagt catcgtcact tccacttttt tgaatcccgg
     6361 cagggccagc accacccgtt gagcttcttc tttcaacgcg tcgcgcaccg ggcaggcggg
     6421 ggtagtcagt tcaacggtaa aggccacgtt gccgtcggcc acccggaggt ttttgacaaa
     6481 gcccagactg acgatgtcgc ggcccaggtc gggatcgtga accttccgca gggcgtctaa
     6541 gatttcagtt tcgcgctcaa tggccatggg acgctccggt catttcagcg gttgccgact
     6601 caacggtttt gctcttgagc accttacaac tcctttgagt ggggaaaacg gttctttccc
     6661 ctccctcaaa tgtaatataa cctaaataga aatgagtatg aactctatcc ggagttcggt
     6721 gggtcctaca gaaagccgca agtaagcaac gggaggattc gagttattcc tgcgagaaaa
     6781 aatggtttgt ctccccttct caattacccg gtctcggatt tcaagactta atattgtagt
     6841 cgactattgt gaatcaaacg tagtcaaata ataatataca ataaaaaaca gttgaatatc
     6901 gtattattca cagaaaacgt aattaaccag aaagcgaggc ggatttatga gaaatggaga
     6961 acgagagtcg gtaagcggca gggaatcact tttggggttg ttattgaaag atcgggagag
     7021 gtttttcagc gaggtcgccg ataacgtcgg gataaaagag aaactgaaat ttcttctgac
     7081 cactctcatc ggtctttccg cgttttacgg cgcaaccgtg ggactgtacc agggcggctg
     7141 gcaaattctc tcggcggcgc taaaaattcc tttccttttc ctcgtcaccc tgcttatctc
     7201 ctttcccctg ttttacatcg tgcagttgct cttaggctcc aagttgagtt tgctccaggt
     7261 gctgaccctc atcctctccg ctctggcttt gacctccctc cttctggccg ctgcagtgcc
     7321 gatctcgctt ttcttcttat tgaccggaga caattattac tttctgcatc tgctgcacat
     7381 tgccgtcttc gctttcgccg gtcttttcgg catgttcacc ctgcacgccg ggctcaccgt
     7441 actgtgtgaa agtagaggtg tctacccgcg cctgggcatc ggcattctgc ggatctgggt
     7501 cattgttttc gccttcgtgg ggattcaact ggcctggcac ctgcggcctt ttgtcggtaa
     7561 aaggaatgag ccgtttgcga ttcgaaagca gtacgagggg aatttctact cggcggtgat
     7621 ctacgcggcg ggtcagctct tcaagggtga tacgctgacg acaccaacgt ttcacccacc
     7681 gagcgagaat ccagctcggt agccgtgcgg ttcagaaaac catgactgac ctcgatttga
     7741 tgaccgtcga agaagtagcg cagtacctgc ggctgaaacc gcagacgatt tacaagtggg
     7801 cgcaggaaaa gaaaattccc gccgtgaagt tagggaagga atggcgtttt cgaaaacgga
     7861 ttttggacga atggctggac ggccagattt cgcataaaca agaagagcgt gactccggcc
     7921 agcatgagag ttgaattccc ttatccgtcg accaacccct gaattatttt tttctcgttt
     7981 tttccagatc cagcatggcg gctttcagtt tcaatccgcc gccgaagccg gtgagcgatc
     8041 catttgcgcc aatcacccgg tggcagggaa tgaccaccgg caaaggattg gagccgcagg
     8101 ccaggccgac ggcacggaaa gccttcgagc ggccaatttt tttggcgatt tcgccgtaac
     8161 tggcggtttt gccgtaggga atcgcgcgca gtttccgcaa cacccggctc tggaacgggg
     8221 tcgagcgcag gtcgagccgg aagttaaact tcttccgttt cccttcgaag tattcccgca
     8281 actgccgggc cggcttttca ttcacccgtc cctgggtcga gatttcggct ttgggaaacg
     8341 tcttgaccag ccacgaaaag aaggctttgt ggttttcacc cggcagggca atgcgggcaa
     8401 cgccgcgcgg ggtggaagcg agatgcacgg tccccatccg cgtggcgacg ctggtgacgt
     8461 agagtttttc cgttgctttc gagcgctttt tcataaagct cctttcagga tttaggagtt
     8521 aggatttggg aattaggggt aatcgcttcg acagagcata ccacaggtaa aaggttgcca
     8581 gactacggta cggtttccag cgttcgccca gcttgcgcac ttggtcttcg gtggcgcgct
     8641 tcttaaaccg gtaaaaacgg gtgaccgcat tctgcaggcc ggtgtcgccg gccggcaggg
     8701 catcaagata accaattccc cgcattaagc aatactcggc agtccagcgg ccgatgccgc
     8761 gaatcgccat caattgctct accgcccggt cgggttcgag ataccggtac gattccaggt
     8821 cgatttcacc cgaagccacc tgccgggcca ggtcgagaat gtagcggctc ttctggcggg
     8881 agaattgcat cttccgcaat ttttcggggt caactgtggc gagccgcccg gcagccggaa
     8941 aaccgtagaa aatctttccg cgataaacca cccgcgggcc gaaagcttcg accagtcgcc
     9001 gcttgagcgt cgtggcaaag gtcagattga cctgctggcc cataatggca atggccaggt
     9061 tctcgaaaag ggtcgagccg atcagtttcg gtttgagacc gcgaaacgtt ttgccaatgc
     9121 gcgataaaac ttcatctccg gcgacggcgc gatagaactt tttcaggtca acgtgcagag
     9181 aaaaagtgcg ggagacaatc cgggtcgctt tttctaattg tgttcgagac aacctcccgt
     9241 tgagaaagcg gagcgagagt tgcggctttt ggttggttcc cgagttgatg agtcggtaga
     9301 gaaccggcgt gctctccagt tcaatgaccc ggtggaaggc gccattcttc accacgtcca
     9361 gaatttcgtg ggggttatga ccccagtggt ggagaatcag gtcaaagcgg tagggccgga
     9421 ctggtcgaag cgtaatggaa gacaaaggca aacccttacg attttaggcc acgccattca
     9481 acgggacata cggtgccgca ttgagagata aatccgatct ttcaccacgg gacagatgga
     9541 aagcaccggc ggcggcaatc atcgccgcgt tgtcggtgca gaactggggg acaggtacga
     9601 agaacttcat acctcgtcgg ctcgcttcgt caccgagcct ttgtctgagt ctccggttcc
     9661 gcgccacgcc gccggccacg gcgatggcgg aaacgttcga ttcttcagcc gcgcgcaccg
     9721 ttttcgcaac cagaacatcc accaccgctt cctggaaacc ggcgcagagg tggggcaaga
     9781 gattctcccg ttctctctcg gacaattttt gcacgtagag ggcgacggca gttttgagac
     9841 cgctgaaact gaaatcgtat gcttcctttt ccaggtaggc gcgcgggaag gtgattctgg
     9901 ccggattgcc ggtctgggcc aaatcgtcaa tcttctgccc gcccgggtag cccgcaccca
     9961 gaagtttcgc cactttgtca aacgcttcgc cggcggcatc gtcacgagtt tgccccagaa
    10021 gtcggtatac gccccagttt ttcaccagca ccaaaagcgt gtgccccccg gagacgacca
    10081 gacagacgaa cggtggctcc agttccgggt gctccagggc attggagaaa atgtgccctt
    10141 ccaagtggtt cacgccgata atcgggaggc ggcgcgacac cgccaccgcc ttggcaaacg
    10201 aaatccccac caaaagcgcc ccgaccagcc cgggaccgaa agtgacagcc agcccctcta
    10261 ttttttccag aggagtttta gcttcggcca gcgcctggtc aacgatcggc accaggttgc
    10321 gcatatgttc gcgggaggcc agttccggca ccacgccgcc gtagggctgg tgcaccacct
    10381 gcgagaggat gacgttggac agaagcgtcc gccgatcttt gagaaccccg gcggcggtct
    10441 cgtcacaaga cgtttcgata cctaaaagca gcactactca atccgcactt ccaccgagtc
    10501 gagaggggcg tccacgacca ccggaggcgg tacgctgacc tgagggacga gccagtgtcg
    10561 gcccgctttg agcctggagc agttcaccac cgccttgagc gtcttgcggt cgatttttcg
    10621 gagggcgctt tcggggcctt ccaaagtcag gcgcaggagg ttcggcctga tccaagctac
    10681 ccgctgcagg gccgagatga gcgtcttgac cggaatcgaa tcgaggacga gactgctcac
    10741 tttctcgact ggtgcacgga cgatgaccgt cggcgttacg agcgaaacgt taaagctatc
    10801 gggggccaga agcctgaggg tggccaccaa aggcgtttgg gtgccccgca ggtgggcggt
    10861 atcggtgaac accttttcga ttttggcgag ggcctgctgc gggccgcgga cggtggccag
    10921 cacaggttca aaagtgagtt cgccgttcca gaaatagccc aaggccggtt cgacggtcac
    10981 ccggctttcg atcttaacgg ttttctcctc gacgcgatcc aaggagaagg tcagcgactt
    11041 cggttggatc aattccaaga tttccagttc cggatccagc gcctgccgca gatcggcctg
    11101 ccgcaccagg cggtcgtaat tgccggggcc caagaggtcg agtttcaccg gcaccgcttt
    11161 cttctgccag atggccttga gcaccgcttt gcccgtgccg cgcacccgca cctccacggc
    11221 gggaggcggc aacttgagga agaccagact cgaatcgagc cccgtgtagg tcaaaggcac
    11281 cgccagagag tgttccaccc gtctttcggt ggagacgtga aaccaaagga tcaccgccaa
    11341 gagcagggcg gcaactttca ccggcaggtt ttcccacagg ggtttgacca tcacgacgat
    11401 tatacctgct cgatttacgg ttgacaaccc gcctgcggcg gatcattcta tccgtaatgg
    11461 cgcaggtcag gtttgaccgg gtcaccaaaa tttttgacag tcagcaaccc gcactcacga
    11521 atttctttct ggaagtcaag gacggcgaat ttgtagttct ggtcgggcct tcgggttgcg
    11581 ggaaaacgac ggctttgcgt cttttggccg gtttggaaga gccgaccgca ggtgacctgt
    11641 tcatcggtga gcgccgcgtc aacacaatac cgcccaaaga ccgggacgtg gcgatggttt
    11701 ttcaaaacta cgccctctac ccccacatga cggtctatga aaacttggct ttccccttga
    11761 aagtccggca catcgtaaaa aatgagattg accggcgggt gcgggaaact gccgaattgc
    11821 tggggctcgc cccgcttctc tcccgcaaac ccaaagcgct ctccggcgac gaaaggcagc
    11881 gggtggcggt ggggcgggcc ctggtgcgcc agccgcaggt ctttctcttt gacgagccgc
    11941 tttccaatct ggacgcggct ctgcggagtc agttgcgggg agagcttttg catttgaaga
    12001 ccaaattggg tgccacggcg ctctacgtca cgcacgatca agtagaagca cttacgctgg
    12061 gcgaccgggt agcggtgctc aaggccggtg aattgcagca ggtaggcagt cccggagagc
    12121 tgtaccggag gccgcagaac ctttttgtgg ccactttcat cggcacgccg ccaatgaatt
    12181 tgattcgggc aagagtgttc cgacaggacg gacttttctg ccacgaggtt ttccggtggc
    12241 gcgtccctcc tccacaagca gaaattttgg aagcctacgt tggtcgggaa atcattttgg
    12301 gactgcgccc cgatattctc cgagttgtgg cgacagacgc tgacgccagc gggatggagc
    12361 gttttccgat ttccatcgat tttatggaac cgatcggcgg cgaaatgctc ttgcacggga
    12421 cagcggccgg gacgccggtg atcataaccg agcggcgggt aatgggagag cggaaggtgt
    12481 caccgggaga gaaaattacg tgcgcgtttg agtggaggca ggtgcaccta tttgatccgg
    12541 catcgggtca gcggatttcg tttccgacac tcaccgtgcc gccgtaactt ccactaccgt
    12601 ctcgttcgta tagcgcaaga cgcgcactgg tctcccaaaa tttttgtttt ctcttatcag
    12661 ttgtgatagg gagttcgaat ccgctttagc gaaagagaag tgttttgttg gccagaagag
    12721 gtaaggcaac tgctcaaagc ccatccggaa aacccaagcc ccctcgtagt tgtccaggat
    12781 gtcaagcgcc agcaccgtcg ttccgggagg ccagttgctt tgcatacggt cgatagtttg
    12841 gatgacattt tcactgatcg ctccagcttt ttcccaacag ccaacgcggg tatggagccg
    12901 gaaaacgtta tacccaaccc agagcaagaa caaagcaaga acagttcctg cggccaaccg
    12961 ttgagttctc gcagtcgacc aaaattttct cagcagaggt aggagaaagg taacccatgc
    13021 tgttaccaga ccgaagaggg gcaagtagag aatgcgttcc ccggttcctt tcaaaagaga
    13081 acccggtaga aaaaacagaa tagcccaaag gagcatcaac cgtaccgcct tcccgccacg
    13141 ccaccagaga agaatgtaga gaaacattac cacgatgatt gccggaatca ctcccatccg
    13201 ggttatctgc gtgagcagtt gagaaaaatt gtgtgagtca cggaaagcat tccagatttc
    13261 caacaaatgg cggaagtcca gatcaaccaa tgagccagtg aaaagaaaaa ggtagttttt
    13321 caggatgtgc agtccgataa cagggacgta ggcggccgtc gaatcgacgg cgaggagggc
    13381 aggcaggcga tacaaaaagc tcgcgacagc aatcagagta aacgggaaaa tggataggag
    13441 acttgaccgc caatgaaaac ggtcccgtcg ccaaaaatct aagaccgcca ttaccgggac
    13501 aaccataagc gcttcctctt tgcttgagag cgctgccaag agcaggaacc cagtggtaaa
    13561 tccggaaagg agcgttttcc ggcgagcgta ccggagatag atgaggagag tcaacagggt
    13621 aaaggtagtg acgatgagag cggtgcggcc ggacatccag gaaattgcct cactgtggat
    13681 aaagtgaata ctataaagtc cggcggagag aagagccaga gcgggccggt gcgtcaaacg
    13741 aaccaccagc cagtaaccga ggaggcaatt gatcacgtgc agcaacaaat ttactccgtg
    13801 ccacgcgata ggactccacc cggtaagatg aaaattcagg taacagatga ggaaaatcac
    13861 ggggcggtat aaactggttg gtcgggaaaa gttttgtaga acgtcagtaa aagacattgt
    13921 tgaagaaaga cgcagaaacc aaaaatcgtc ggagagaaac tggtggttga ggttgcggcc
    13981 gtaggtgaac gccaaaacca agatgacgaa aacgataaag ccagcggcca gccagaagcg
    14041 tgctttgggg cgcgaactgg tggcagggat ttcaactctc atggaaaaga gaatttacaa
    14101 ggatttggcc ggaagaagaa acggttgtta cgggagaatt ccggtggggt cgacctgact
    14161 tttgacctga ttttccagtg cggcaagggc gggatcgcgc tttgcttgac cccagggcca
    14221 aatcgggtcg atcagtcttg ccttttcgta ctgtttctgt aagcgcaacg ggatcaagcg
    14281 aagcgtcact ttggtaatca ccgccaggcg gccccagctg ccgcagagga agcgggccac
    14341 gtcgtacccg gccacgcttt tgtaggtttt ggcacccagt ttgaccagtt caccgtagcc
    14401 ggtcaccgcc tccagcgcca taaggtaggt tttggtctgg atcgtatcgt cttctaatgt
    14461 tgccgaaaga ttcatcgcta cgctcccgcc gactgtaccg ttgtatcccg cctgactcag
    14521 cggataggca actttgtgcg gcgatagagt ctgattcaaa acccctaagg gaaaaccggc
    14581 ctctagagtg acatagagat cgtcggaagc cagttccacc acgcggttga ggcgctcgag
    14641 gctgaccacg actcgatttg gatccgatac cggcagatga gcgccatttc cctgaatggc
    14701 cagaggaatt ttcctcccgc aggcaaaagc gacgatagat ttgacctcag ctgccgcgcg
    14761 ggggcagacg gcgggcggag ttttttgcag gaaaagattt tccgagccaa caaagtcggc
    14821 cagctgggtc aatgtgcgat ttgaggtcaa gggtgtggtc acgccaaggc cgccataatg
    14881 ccgtcggcca caaaccagcc ctcggcttgt aactggagat ggccgtcggt gcgcttgagg
    14941 tatcccaggc ggatgaaacg ctcaatttct ttctgcttgt ctaattcgag ccgcgttcca
    15001 aaacgaaggg cgtagttttc caagtcaatt ccctcagcac ggcgcagacc caaaaggatt
    15061 gcctcggtcc gttcctcgac cggcgaaagc gtttcgataa agtcgcgggg caatttaccc
    15121 tcgacaattc gagcggtata gaccttcaga tcgcggacat tcgcccagcg ttgattccgg
    15181 aggaatgaat gggctgaagg gccaaagccc aggtactccc ggtcggtcca gtatttttca
    15241 ttgtgccggg agcggaagcc gggcttggcc cagttggatg tttcgtagtg ttcgtagccg
    15301 gcctcggcca agagtatccg agcggcggcg aacatctggc gggaaatctc ctcgtcgggc
    15361 aaatgcaaac gtccctgctg gaaataaaga gcgtaaggag tgcgggtttc aatcgtcaag
    15421 cagtaagcgg agatgtgctc aggtgaaagt gcgatcgctc tggccagcgt ttgttcccat
    15481 tcagccagag tttgatccga cagagcgtag atgaaatcga gactaatgtt cttgaacccg
    15541 gcgcggcggg cccaaagaca ggcccgctcc gcctcagcgg cggtgtggtc gcggcctaaa
    15601 atcttcaggt ggtggtcgaa gatgctctgg tagcccattg aaatgcggtt gaaacccgag
    15661 gaacgaaaag caccgaattt ctcctcggtg acggtgccgg gattggcttc cagtgttatc
    15721 tctacctgcg ggtcgatctg gaaaaaaagg cggagcgtcg tcagaatttt ctgcagcgat
    15781 tcgatggcaa ggattgaagg agttccgccg ccgaagtaga tggtgtcaac gggacaaccg
    15841 gcaaccccaa ttgtcgttgc ggtcatttca atctcgcggc agagggcgga gacgaattcc
    15901 tcttcacctt tgcccttgaa tacgatcgag tagaagtcgc agtaggggca cttgaccgtg
    15961 cagaacggca gatggcagta aatgcctaaa gtggccatag gtgggtgtcc aaaagcatag
    16021 tcatcgcgga tacgctggtc aaactcttaa cgaacgcatc aatctagaac tttgcgatcg
    16081 gttcatcacg ctcttcgtat cgaagttcct ccaaaacgag aggctgcacc cattcttgaa
    16141 gctcatgcaa tgaactaaat ctcggtaggg gcttatcgta ccacggatcg ctctgtagga
    16201 tcgcccaagc gaatgcatca caacttctac ggggatatag gaagattaga ctctttcgtg
    16261 gctccgtgaa tgtcccatag tgttctctgg taatgaattc tttccaattt tcagatagtt
    16321 catttctacc tttaatatca atcaattcca tttcggacaa ttcacgccat tcgtttttat
    16381 cccatgccag tttcatcaag gattttgcat ttatgtctgc cgaaggtgca ctatagccga
    16441 aaatcgttac tatgaaagcg tacttcaggt gtgcttctaa ctcaagccat tcgttcgcta
    16501 taaaaggacg ttctttgtaa tttttgtctc gaatcggaaa aagtaaaggc gattttgtaa
    16561 atggcttgtc acatttgctg caccttcggt cagcataacc tttcacttta tcgttgtcgc
    16621 aagctccgac accaacgttt ccgtgcaaga aaacgatttt aggtaatccc ttgatgtgtg
    16681 aattgcgccg atatgcttgt attagcagag gatcccaatt gaacgaggca attatatcct
    16741 ttgctcgcaa acaaagaatt agctcatcgt aaagtgtcgg tttatctggt atcatcattt
    16801 gagcaaagta ctcgtaaacc aaggtttcta gttgctgtaa tatctgccgt cgatcagttt
    16861 ggcgatacaa tctgtcataa atctcctcaa agttccggtc aggatgttca acgccatgct
    16921 ccgatagaaa ttcccctatt cctaccgtct caacaaagtc agtcattaaa ggtaactttc
    16981 gtccatattt gtcgccttgc gggaaacttg cacggctagc gcccgcgcct aaaataacgg
    17041 catgaggcgc ttcgtttacg ttcttgtcta tccttattgg aggtagcttg cccatagggt
    17101 actgacaaaa aaatgattgt ctggtcagct aatcggaagt cattctaacc gtgtttccct
    17161 tgcttcagcc aatcggaata cgaaatagca atgccagttt ctaagaacga cttcaacgaa
    17221 tccattgccc agctccagcc ctgtttcaat ttatcagcgt ctttcttcgt tttcagtttc
    17281 tcatgagtca gtcgaacagc agtgcaattt tctccttgct gggaaaattc aacgttaacg
    17341 cggctttccg gctgaggctg tttattctcc caggtaaaac ggatcagtct gttcggcacg
    17401 attttcaaaa attctccgga gtcgccgtcg ctgttttcgt accgtccgcc gacctggaag
    17461 tcccgtcggg ccgtggtggt gaaccagcgg gagaggagag ccggatcggc ccacgcggcg
    17521 tacgctcgtt ttatccccgt agcgatggtt ctggtgacgg tcacggccaa atcctcatcc
    17581 ggcggttctg catttaccag cgcatcagct gctcgatggt ggtgaccgct gccgggtcgt
    17641 cgggaaagat gacgggcagt tctttggtgc gcaccgtccg gtctcccaca cgcttcacca
    17701 ttaccttcac ggggcagccg caggcgctgt agatgaacgt cacttcgcgc tcggcgccat
    17761 cgggccagat ggatgccacc ggccggccca attcccgaac gttggccgtc agtatggagc
    17821 tgggattgtt ggcaccgacg acggtcaacg acttgccctt gccatcgtcc acgaaaatcg
    17881 aaagattttt gccggtgaac gccgcattcg ggttcgggga aggcctcatc tcgcactgcc
    17941 acagaatttc tccagtgcgc cggaagacca gcgagcccct ccccggcttt tccgtgctca
    18001 cgtgcatgga gaggtgcagc ttcccctccg cggtgggttc tccgtaggcg tcgataaccg
    18061 caaacgtccg ccccactcca tcgaaaggct ccgagatgcg ttcaatgccg cgcagcgctt
    18121 cggcgatagc gggcggcttc cctccgaagt cgagctccac ggtgaccatc accgcctggt
    18181 tcacttccaa attcatcaat tggggtgccg ggttaagttt tggcttttga tccggttggc
    18241 ccggcagcgt gacggcttcc gggtttttgt ccgtcgccag cggaccggtt gtttctttcg
    18301 agggtttcgc tacccggttt cttcttgctg tgaataagaa gatgctccca actatcacca
    18361 ttgttagaat aattgtggcg gtgagccgcc gtttgaattt tgaaggagaa gccgggtgaa
    18421 tcgttggctc tgctccgccc gggtaatttt ttttcttgtt tttgggcatg gaactattta
    18481 ccctcaggct gtcccaccat ccgccgtagg aagaacggta aatcgtttcg tctgcctcag
    18541 gctcgatttt ttccgcaccg ccgggtcaac ggtttttccc gaactgtgcg tgtgcccttt
    18601 tctcccggtc accctgcttt tttcaacagc gcgatttgac cgacatggta gatgtcatgc
    18661 tggatgatgc cgtggagcag atagtaaacg ctgtaattcc ggccacgtat gagctcctcc
    18721 aaccgggaat cggtgagtcc ggagactact tttcgcagtg cccggtggcc attctccagc
    18781 attttcattg ctttttgcca agccgactca ctggtgtctc ttataggagg ataatcttcc
    18841 tcgtcggtaa ggtcgacgtt gtctccctct attcttctgc gcacagcgtt ttcccaggcg
    18901 gcaatgtgca ggacaagttc ccagatgctg tgagcatttg ggatgggttt tttagctgct
    18961 ttttcagccg atacgtcttt taaaacttct cgaaccgaag gcccgtgcca ggcatcgccc
    19021 tcaaaaacac ggttcaactg atccgctatt cgttcgactt cagtcacaag tcctccggat
    19081 tttggtcacg cttccagttt ggcatccatt gtaatctttg tgtttaacag gcgcgagatg
    19141 gggcagccgg ctttggcgtc gttggcggct ttttcaaaag cggccttgtt tgcgcccgga
    19201 attctgacgg ttgtttctaa atggattgcg gttatggttg gaccgccttc cagcatttcc
    19261 agagtaagtg tcgcagccgt gttgatgctc tgcggggtca ggttggcttt gccgagttcg
    19321 gcggaaagcg ccatcgaaaa gcagccggcg tgggccgcgg cgactaattc ttcgggatta
    19381 gtgcctttcc cgctttcaaa gcggccgccg aaggagtact gggtgttcga gaggacaccg
    19441 ctttcggtcg agaccgttcc tttgccgtct tttagaccgc ctttccagac tgcggatgct
    19501 ttgcgtttca tttctcctcc tttaccttta gtcttcggtt gagatcgttt tgccgatttc
    19561 agcttaatac ggcctcttca atcgcgtcga tcttgcctta ggtgcaaatt ctctcaattc
    19621 cttaacccga ttgggagaat ataattactt atatcaagac cgacagcact ctttggaaaa
    19681 agactttaag gcgggatggt ttaactcaag gtaggcacat cggctgaggt tcggcattga
    19741 atcagaattt cttgacaata aaatgctttg gtgtcatttt gggtctgcct gaaataacgt
    19801 gcccgtactt tctgtgatct ttagacgcaa gaagagcctt atcatacgat tattaacctg
    19861 gctactggtc tctggctact ggctttcctt ctttggccgg gccgcagcca cgccgctgga
    19921 ggacatcccg ctggacagct gggtttatcc ggcgttggaa gaattataca gccgagggta
    19981 ctcttacgag ttttttttgt ccgacaagcc ctatagccgg ggcgaagtgg ccagatgtct
    20041 caagaaaata tccgcctcga atccccacct ccttgagaat gattttctca tactccgcct
    20101 gcgctatgag ttcgccgatg aactggccgc actcgaaaac cctcccgaaa cagacaaacc
    20161 gcttgaagcg cggggcggcg cttttgtccg gggtaatggg ataacctctg ggaaagccag
    20221 acccatccag cgcggagcgg ttggcgtctt tttaggattg gaagcggagg acaagttcgt
    20281 gacccgcgcc cggatgaccc tcgacacccg cacgcaggac gacccggcca tccgcggccg
    20341 ccggtggcac aacagcgtga ctttgttctt cgacgatgcc tacgccaagg ttcacacccg
    20401 ctaccttgac ttattctggg gccggcaaca cctgcagtgg ggagtgggga gcgacgcgct
    20461 tttgctgtcg aatcattttc cgcccttgga gcaggtcaaa ctgaaagcca actacaagac
    20521 cctgcgctac cagtttttgg cttcgcggtt ggatccgatt ttcattgaca ctctgggagg
    20581 gaacgttccg cgcttctttt cggcgcaccg gctctcctgg aaaccctggc cgtggctgga
    20641 ggcgggggtt tccgagagca ttgtctacgg cgggccgggc cgcagttttg agctcttcta
    20701 cctcaatccg ctcctgccgt tctacgctga acagtacaat aatttcgtgg acgacaaccc
    20761 cttcgtgggc gtcgattttc tcttctcgcg catccagcac ctcgtcttct tcggggaatt
    20821 gatgattgac gacttccaga tcgatttcaa aagcgagccg cagcagatcg ggttccgcct
    20881 gggcggatgg atcagcgatt ttataagtaa aggccgcacc ttcggccagt tagaatacac
    20941 gcgggtaaat accttcgtct acggacagaa caaaccctgg aacatctaca cccgtttcgg
    21001 cgccgggatg ggaagtgaaa ttggccccga tgccgaccgc tggcggggaa gtttcctctg
    21061 gcgagcccat cggtgggtcg atctcgtcct ttctggagag taccgccggc gcggggaaaa
    21121 tcgtattacc acaccgcaga acgtggcggt gccgtatgaa aaattcccct ccggagtcgt
    21181 cgaaaaaacc gccctggcgt ccttcaccgg gcggctgcag tatcaagcaa atgttttcgc
    21241 cgacctcacc tgcggcggta catttctgcg gaacggcgac aaccgccggg gaaataatcg
    21301 ggatgatttt ttcgtccggg gaacgtttac catcaccttc tggaaaacga agcagtactg
    21361 atgggcaacc agagtttagt ctctctcatg cagcacagtc aggaaacgca atacgccgcc
    21421 tttgccctga agagttttgc gaaggtcaat ctctccct
//
LOCUS       DBNGZD010000439         8883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_69229, whole genome shotgun sequence.
ACCESSION   DBNGZD010000439 DBNGZD010000000
VERSION     DBNGZD010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_69229"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="AC16GM_11510"
     CDS             <1..312
                     /locus_tag="AC16GM_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2924334.1"
                     /translation="LTNNFIIGSLAADPGGVIDAGSAYVYQWFGLISAPPCVARGDVN
                     SDGSTDIVDVVNLIQGVVSVAEGSNSKASDTNCDGTRDIADIVLLIQYVVFGTPTPCC
                     L"
     gene            456..2159
                     /locus_tag="AC16GM_11515"
     CDS             456..2159
                     /locus_tag="AC16GM_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924335.1"
                     /translation="MRVATTFGLLSLFTSSVTFADITGSITDNTSPGLVILGAAAFEQ
                     SRVNDSLYPVLFTVDALFFADSLPPHAFTLQTDPAKTYVVAAIGFSSLSNQGGPDAVG
                     DIHYNVRGDSSGLSLAITPNVNHITGRLLPAGGGTLGNDPQVWAKDAGGTFAGVTFPD
                     SAGNFSFNYLPGAGPYHILSLGDRHYLRVDSNKTNPVNLGDLTLADAGQTFITASSNT
                     GVPGRIRDVLSDRKNYDLTFTVYNAPSGQPLTAVTFEAPGDIGENLPQPVRYYLSPNP
                     GNHTIGPGLDWNSVTVDLSRQPHRVTFARGTGTSSIPPGGSGTFTIRVDSMTNVAYDT
                     YQGWHVAVRSNTGLQGRAQDPDSCRSGGDCGVESVQKILEAYDIRFDPPGQNTGTELA
                     DIDLVLRFWVRNHGNGVFQPYANWTNVFSTSAFAFQSFPTVANSIAAGESLAVLTQSV
                     HTTVIPGSYLLFALVADSTGSNQQNSFAQYTEPLFICVPLGDLAGGPGNCADGIIDLV
                     DLVTAVNYVVFGTPFCGQVGIADVAGDPQNCPDGIVDLVDLVTMVNYVVFGTPFCRAC
                     L"
     gene            2671..4332
                     /locus_tag="AC16GM_11520"
     CDS             2671..4332
                     /locus_tag="AC16GM_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924336.1"
                     /translation="MRVVIVLVGLFFLWISSVTADITGTITDHSSPTHSILGVAAFEQ
                     WRVSDPLFPLLLTVDAAVLADSLPPHGFTIPADPAKTYVLAAIGFVESGDDISPEAIA
                     DIRYNVAGNASGVALAITPNPNHITGRLYSGGGGSLGRDPMIWVQDTSGKFVTVGSPD
                     TAGNFSLNYLPGAGPYTLLSVAERHYVRVDANKVNPSTVGLLGLSPAGQTLITGSANT
                     GVPGQVRAVLSDRKNFELQFTVFNAPFGQALTAVTIEAPGGDPGDPPVRYYLSPVASN
                     HRLPLDLFWTSIVVETNRQPNRITFARGVGTSKIPPGASATFTLRIDSTTNVDRDTYQ
                     DWNVVVRGDTADLGRAQEPDTCSGWEEDCGLETVQRILEPYDLRFDPPSNNDGTEPVD
                     VDRTLRLWVRNCGTGVFHPYPLWTSVDPGGAFSYQQWPPTVVPSLAPGDSVAVLSQSV
                     HTTMYPGTFTLHAFVIDSTGPGVNTSYADYRESLRTCFMRGDVTVDGVIDLSDLVALV
                     QDIVFGVPLPNPTAGDANCDGVRDIVDLALLVQFVVFFSQTPCCL"
     gene            4775..6970
                     /locus_tag="AC16GM_11525"
     CDS             4775..6970
                     /locus_tag="AC16GM_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924337.1"
                     /translation="MRSLVRLGRRSLVFGFLTLTFSLFFIPIPQAAARESSQGSVVVS
                     AFILDWPFVLKYKKEGLARNDNLGTAVAGAGDVNNDGYGDFIVGAPFTDPNGLTDAGS
                     AFVYSGRDGALLFQKDGGAAGDEFGLAVSGAGDVNNDGYDDFIVGAPLADPGALADAG
                     SAYVYSGLNGTLLYQKNGNASGQQLGHAVASAGHVNADAFADFIVGVHAASPNLITSA
                     GIVFVFSGADGSVLHQKNGTELAGQLGYSVAGLGDINGDGFDDFAAGANRASPGFRSN
                     AGSVFVYSGSGGGLLFQKDGTAAGDQLGYSTAGGGDVNNDGVPDLAAGAPGANPGGLN
                     DAGSAYVFSGATGGVLYQKNGIDVSNQFGLSVAIVGDATHDGFAEFLAGAPLADPGGR
                     GFAGTAVLFSGATGDTLLWRAGASAGDQLGFAVAALGDLNADGYADFAIGAPFTDPAD
                     FNLGTVLAFLSGYEMTLTSVANVPDDQGRQFQLNWKSRPTGSGLGQKFQIFRRDSPPF
                     GAVATPPGTWSKIKEVPVVVTESLYTTTVQTPTNREVFFVRNVANDSTVFFDSNPDSG
                     VATDELFPPAPAVTVASTLPCSALVVSWHPVAAPDLEHYEVFRDTIFNLATKIFLANV
                     GRDTVYVDRTALPNQFYFYLVVAQDSNGNRSFSVASGANLQTGDVDGDGLVDIIDVIG
                     LINDVAHGEPLPNPLGGDTDCNGVRDIVDVVLLIQFVVFGSPSPCCLSP"
     gene            7223..8575
                     /locus_tag="AC16GM_11530"
     CDS             7223..8575
                     /locus_tag="AC16GM_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924338.1"
                     /translation="MRVRIFLFTVVIGLLAASVFALVPSVPNLINFQGRLTDASGVPV
                     ADGVHAVTFRIYSTAVGGVLLWDDPVAGGVTTSGGLFTYELGSDPADLLPVDLFQDNN
                     EAWLEVEADAVILSPRTRLISTPYTRVAGSLEVPSAFVPGVVGIATFGNHQLSTYGSD
                     GQEQIRLWGASYGESLLHDADPTNDETVRLTANASSGGELELRDNGGLTTIDLHGGLT
                     GDASAILPTDAINSLEIKDEPGIAHSFTFGSTILPVAPGTIPIESVTITYPANGYVVV
                     TAHGFFKLTHVTGTDTWGRAFISTVRGGIDFDNFALFTVESSHPSFSGDKGYRSFSLT
                     KVEAVTGGTTVKYYLNADRWTPNAIGSLSEINRVHLLAQYFPTAYGTVVSTVASVPSG
                     LEPSAMAGVPGVGSQQSEVRTITVEEHNARLKAEVAEMKARLQTLEEQVKQQNAGGGR
                     "
     gene            8572..>8883
                     /locus_tag="AC16GM_11535"
     CDS             8572..>8883
                     /locus_tag="AC16GM_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924339.1"
                     /translation="MKTKQSNFDSAWWGGRAPGLPLVLIGFLFALSLSAFASDQRKAT
                     GSQLSPVQESKPSEARTTAPLLLELECLSVQGSGATFVTGGGLQLGFSVAQSVAGAQS
                     AA"
BASE COUNT         1648 a         2612 c         2731 g         1892 t
ORIGIN      
        1 ttaacgaata attttattat cgggtcactg gctgccgatc cggggggagt catagatgcc
       61 gggtccgcgt acgtgtatca gtggttcggc ttgattagcg cacctccctg cgttgcgcgc
      121 ggggacgtga atagcgacgg gtcaaccgac attgtggatg tcgtcaactt gattcaaggc
      181 gtggtatccg tggctgaggg gtcaaattcc aaagccagcg acacgaactg cgatgggacg
      241 cgggacatag cggacatcgt gctcttgatc cagtacgtcg tgttcggcac gccgaccccg
      301 tgctgtttgt aacggaattt ccacgttctt agctccctga tagaagttct tgctttcctt
      361 caccccttca cgttattttt cccatgctgt cactggattg gcagttgccc gcagatgtct
      421 gcggcgcagg gaaaccggaa acgaaaggac aggttatgcg cgttgcgact acgttcggcc
      481 tgctttctct tttcacttcc tcagtcacct tcgcggacat caccggctca attaccgaca
      541 acacctcgcc ggggctcgtt atacttggcg cggcggcctt tgagcaatca cgggtcaatg
      601 attcactcta cccggtgctg ttcactgttg acgccctgtt ctttgccgat tccctcccac
      661 cgcacgcctt cacgttgcaa accgatccgg ccaagaccta cgtcgtggca gccatcggtt
      721 tcagttcgct ctcaaatcaa ggcgggccgg atgcggtggg tgatatccac tacaacgtgc
      781 gcggtgattc gagcgggctc tccctggcga ttacccccaa cgtaaaccat attaccggac
      841 gtcttttacc cgccgggggc gggacgctgg gaaacgatcc gcaggtctgg gcgaaagacg
      901 caggcggaac ctttgcggga gtaacttttc ccgattcggc ggggaatttc agtttcaact
      961 acctcccggg tgccgggccg taccatatcc tttcccttgg tgatcggcac tacctgcgcg
     1021 ttgattcgaa caaaaccaat cctgtgaact taggcgatct tactctggcg gacgcgggcc
     1081 agaccttcat taccgccagc agcaacaccg gcgtgccggg ccggatacgg gacgtacttt
     1141 cggaccggaa aaattatgat ttgacgttca ccgtttacaa tgctccctcc ggccaaccgc
     1201 tcaccgcggt gaccttcgaa gcgcccgggg acatcggaga gaatctgccc cagccggtgc
     1261 gctactacct ttcgcccaat ccgggcaacc acaccattgg cccggggctc gattggaact
     1321 cggtcaccgt cgacctgagc cggcagcccc accgcgtcac gtttgcccgg gggaccggaa
     1381 cgagcagcat tccgccgggt gggtcaggca cttttacgat tcgcgtcgat tcgatgacca
     1441 acgtcgctta cgacacctac cagggctggc atgtggcggt gcggagcaac accgggcttc
     1501 agggccgggc gcaggatccc gactcctgcc ggagcggggg agactgcggg gttgagtcgg
     1561 tccagaagat tttggaagcg tatgacatcc gctttgaccc gccggggcag aacaccggca
     1621 cggagttagc cgacatcgac ctggtgctcc ggttctgggt gcggaatcac ggcaacggcg
     1681 tgttccagcc ctatgccaac tggaccaacg ttttcagcac cagtgcgttc gccttccaga
     1741 gtttcccgac cgtcgccaat tcgattgcgg cgggtgagtc gctggccgtg ctcacgcagt
     1801 cggtccacac caccgtcatt cccgggtcat acctgctttt tgccttggtg gctgattcca
     1861 ccggctccaa ccagcagaat agttttgccc agtacacaga accgctcttc atctgcgtgc
     1921 cgttaggcga cctggcaggc ggtccgggta actgtgcgga cgggatcatc gatcttgtgg
     1981 acttggtgac ggcggtgaac tacgttgtct tcgggacgcc tttctgcggg caggtgggca
     2041 ttgcggacgt ggcgggggat ccgcagaact gtccggacgg gatcgtcgac ctcgtggatt
     2101 tagtgacgat ggtcaactac gtggtgttcg ggacgccttt ctgtagagca tgtttgtaaa
     2161 aatgagtgat gggtgactga tgaggggtga cgacccctcc ctcgccctcc cctcagaggg
     2221 gagggaaaag ccattatcaa aatagggatc aggtcggtac gacccagcca aaggcggggg
     2281 attagaaaac aattccattc cataggtcag acatcttgtc tgacccctcc ccagccctcc
     2341 tcggtacgag tcttcgaccc tcaaagggga ggaagagtca ttatcaaaac agggatgagg
     2401 tcggtacgac cccggccaga ggcgggggat cagcgatcag gggtcaggag agaggcaaag
     2461 gagttaaccg ccagcccccc cttcgtgacg gtggatcttc gaacccgtgc cgccgtgagt
     2521 gagaagcgta cgaaagcagt ccggtaattc cccattttta cttttctgat ataataatcc
     2581 gctatatatt tcctttcgcg ggggacggac gcggggtgcg ttcgtacccc cggccttgcc
     2641 ggttcgcaag gcaaaagaaa ggagtgttct atgcgcgtcg ttattgtgct ggtgggattg
     2701 ttcttccttt ggatttcttc ggtcacagcc gacatcaccg gcaccatcac cgatcattcg
     2761 tcgccgacgc actcaattct gggcgtggcg gcatttgagc agtggcgggt cagtgacccg
     2821 ctttttcccc ttttgctcac cgtcgatgct gcggtcttgg ccgattcact gccgccgcac
     2881 ggctttacga ttccggctga tccggctaaa acctacgtct tagctgccat tggctttgtg
     2941 gaatcggggg atgacatcag cccggaggca attgccgata tccgctacaa cgtggccggg
     3001 aatgccagcg gggtggcgct cgcgattact cccaacccga accacattac cggcaggctc
     3061 tacagcggcg ggggcggttc cttggggcgg gatccgatga tctgggtgca ggacaccagc
     3121 gggaaattcg tcaccgtcgg ttctcccgat acggcaggga atttcagtct gaactacctg
     3181 cccggggccg ggccgtatac gctcctttcg gttgccgagc gccactacgt gcgggttgat
     3241 gcgaacaaag tcaatccttc caccgtggga cttctgggac tgtcgccggc ggggcagacg
     3301 ctcattaccg gcagcgcgaa taccggcgtg ccgggccagg tgcgtgcggt cctctccgac
     3361 cgcaagaatt ttgaactgca gtttaccgtc ttcaatgcgc ctttcggcca ggcgctgacg
     3421 gcagtgacga tcgaagcgcc gggaggtgat ccgggagatc cgccggtccg ctactacctg
     3481 tcgccggtcg cctccaacca ccgtctgccg ctggatttgt tctggacctc aattgtggtc
     3541 gagaccaacc ggcagccgaa ccggatcact ttcgcgcggg gggtcgggac gagcaagatt
     3601 ccgcccgggg cttcggcaac ctttacgctg cgcattgatt cgacgaccaa cgtcgaccgc
     3661 gacacctacc aggactggaa cgtggtggtg cggggcgata cggctgattt gggccgggca
     3721 caggagcccg atacctgctc cggctgggag gaagactgcg gcttggaaac ggtgcagagg
     3781 attttagagc catacgatct ccgcttcgat ccgcccagca acaacgacgg caccgagccg
     3841 gtcgacgtcg accggacgct ccggctctgg gtgcgcaatt gcggcaccgg ggtcttccac
     3901 ccctatcccc tctggacctc ggttgacccg ggcggcgctt tttcctacca gcagtggccg
     3961 ccaacggtgg taccttccct tgctccaggt gactcggtgg cggtgctctc ccaaagcgtg
     4021 cacaccacga tgtatccggg aacgttcacg ctgcacgcct tcgtgattga ttcgaccggc
     4081 ccgggggtca acaccagtta cgccgattac cgcgagtcgc tcagaacctg ctttatgcgc
     4141 ggggacgtga ctgtagacgg cgtgatcgac ctgagcgacc tggtggcgct ggtgcaggat
     4201 atcgtgttcg gagttcccct gcccaatccc accgccgggg atgcgaactg tgacggggta
     4261 cgcgatatcg ttgatctggc gcttttggtg cagttcgtgg tgttcttcag ccagacgccg
     4321 tgttgtttgt agacacaggt tgtgcgtagg ggcgaggtct cccggcccta aaacggggtt
     4381 acccctctcc ggcgagggaa cctgccgcta ccaaaccggt ttcgggaaaa gcagctccat
     4441 tccgtctcag gcggatcagg tcggcacgac cccgccccca cgggcagtat gcccagtcaa
     4501 tacgttcaga ctcccagacg ggccgtatgg ccagaggcgg ggaatcaggg attagaaagg
     4561 caattgaact cagccacagt gttcggctgc tttttctttg ctttttccat ccggctcgct
     4621 atgttcaact ccggctcagg tgagagaccg gggtatgctc gaaacataac ccggcccgca
     4681 cgggggagcc cgcatggaga gcagggatta gagatcaggg atcagggatt agtgatccct
     4741 gcgttcaaca cgtgcgtaac aacgggaggt tgcaatgcgg agcttggttc gcctgggtcg
     4801 tcggtcactg gtttttggtt tcctcacact cacttttagc ctctttttca tccccatccc
     4861 acaagcggct gccagggaga gcagtcaggg atcagttgtc gtctcggcct tcatcctgga
     4921 ctggccgttt gtgctgaagt acaagaagga gggtttggcc cgaaacgaca atctggggac
     4981 agcggtcgcc ggggcggggg acgtgaataa cgacggttac ggcgatttca tcgtcggcgc
     5041 gccctttacc gatcccaatg gtctaactga cgcggggagc gctttcgttt actccggccg
     5101 ggacggggcg ctcctcttcc agaaagacgg gggagccgcg ggggacgagt ttggattagc
     5161 cgtctccggc gccggtgatg tcaataacga cggctatgac gatttcatcg tcggcgcgcc
     5221 tttggccgat ccgggcgcac tggccgatgc cgggagcgcc tacgtctatt cgggcttgaa
     5281 cggaacgctc ctgtaccaga agaatggtaa cgcctccggc cagcaattgg gccatgcggt
     5341 ggccagtgcc ggacacgtca atgccgatgc atttgccgat ttcatcgtcg gcgtgcacgc
     5401 cgccagtccg aacctcatca cctccgccgg aatcgtgttc gtcttctcgg gagcggacgg
     5461 gtcggtgctc caccagaaga acggcaccga attggccggg cagctgggat attcggtggc
     5521 gggtctgggg gacatcaacg gcgacggctt cgatgatttt gcggcggggg ccaatcgggc
     5581 cagccccggg ttccgctcca acgccggcag cgtttttgtc tattccggat cgggcggcgg
     5641 gctcttgttc caaaaagatg gaaccgctgc cggggaccaa ctggggtaca gcacggccgg
     5701 gggcggcgac gtgaataatg acggcgtgcc ggatctggct gccggtgcgc cgggtgccaa
     5761 tccgggcggg ctgaacgacg ccggcagcgc ctacgttttt tccggcgcga ccggcggggt
     5821 gctctaccag aaaaacggca ttgatgtttc gaatcaattc ggcttgtccg tggcgattgt
     5881 gggcgacgcc acgcacgacg gattcgccga gtttttggcc ggcgcgcctt tggccgatcc
     5941 cggaggacgc ggatttgccg gcacggcggt tcttttttcc ggggccaccg gtgataccct
     6001 cctttggcgg gccggcgcca gcgctggcga tcagctgggc tttgcggtgg cggctctggg
     6061 cgacctgaac gccgacggct atgcggactt cgccatcggc gcgcccttta ccgatccggc
     6121 cgatttcaac ctgggcactg ttttggcctt tctctccggc tacgagatga ctttgacctc
     6181 ggtcgccaac gtgcctgatg accagggccg ccagttccaa ttgaactgga agagccgtcc
     6241 cacggggagc gggctgggcc agaaattcca aatcttccgg cgcgattccc cgcccttcgg
     6301 cgcggttgcc actcccccgg ggacgtggag caagatcaaa gaagtgccgg tcgtcgtgac
     6361 ggaaagtctc tacaccacca cggtacagac gccgaccaac cgggaggtct ttttcgttcg
     6421 aaacgtagcc aacgacagta ccgttttctt cgattcaaat cccgactccg gcgtggcgac
     6481 cgacgaactg ttcccgccgg ctccggcggt gacggttgcg tcgacgctcc cctgttcagc
     6541 tttggtcgtc tcctggcatc cggtggcggc gccggacctc gagcactatg aggtcttccg
     6601 cgacaccatc ttcaacttag ccaccaagat tttcctcgcc aatgtcggcc gcgatacggt
     6661 ctatgtcgac cggacggctc tgcccaacca gttctacttc tacttggtgg tcgcccagga
     6721 ttccaacggg aataggtcgt tctcggtggc gtccggcgcc aatctccaga ccggcgatgt
     6781 ggacggcgac gggctggtgg acatcatcga tgtgatcggt ttgatcaacg acgtggcgca
     6841 cggggagccg ttgccgaacc ctttaggtgg cgataccgac tgtaacggcg tccgcgacat
     6901 cgtggacgtg gtgctgctga tccagttcgt ggtgttcggt tcaccctcgc cctgctgttt
     6961 gtcgccgtaa ggtgaatcgc catgttcggc gagagttttg ttgcacggca atcgaagact
     7021 tattatctct tgattcaaag attctccacc tgcttgaaga agtgggggag aatcaataag
     7081 agagcagcgg atggctcaac ggggaggtaa agtatggcga actgaccaga atggttccgg
     7141 ctgatcgttc aggcgattgc cagcaatcgc ggcaacaatt tggcgcaccg gctggcgggt
     7201 gcaccgcaaa cggaggtttg caatgcgagt acggattttt ctcttcactg tggtcatcgg
     7261 ccttctggcc gcaagtgttt tcgctttagt gccttcagtg cccaacctga ttaacttcca
     7321 ggggcggctg accgacgcct ccggcgtgcc ggtggccgac ggcgtgcacg ccgtcacctt
     7381 ccgcatctac agcacagcgg tgggcggcgt cctactctgg gacgatccag tggctggagg
     7441 ggtgacgact tccggtggtc tcttcacgta tgaattaggt tcggacccgg ctgacctctt
     7501 gccggtggac cttttccagg acaacaatga ggcctggctg gaagtagaag ccgacgcggt
     7561 gatattatct ccgcgtaccc ggctgatcag cactccctac acgcgggtgg cggggagtct
     7621 tgaggttccg tccgccttcg tcccgggagt cgtgggtatt gcgacttttg ggaaccacca
     7681 gctttccaca tacggcagtg acgggcagga gcagatccgg ctgtgggggg ctagttacgg
     7741 ggaaagtctc ttgcacgacg ctgatccaac caacgacgaa accgtaagac ttaccgccaa
     7801 cgcttcgtca ggtggtgaat tggagctgcg agataacggc gggctcacga ccattgatct
     7861 tcacggcggg ctgaccggtg atgcctcggc cattctcccc accgacgcca tcaactcgct
     7921 ggagataaag gacgagccgg ggatagcgca cagtttcacg ttcggtagca ccatattgcc
     7981 cgtcgctccg gggacgatcc cgattgaatc ggttaccatc acctatcctg ccaatgggta
     8041 cgtggtcgta acggcacacg gcttctttaa gcttacccat gtaacgggga cagatacttg
     8101 gggacgggcc ttcatttcca ccgtaagggg cggcattgac tttgacaact tcgcactttt
     8161 taccgtagag agcagtcacc catcgttctc cggggacaag ggctaccgct cattcagtct
     8221 gaccaaagtc gaggccgtca ccggtggaac caccgtaaag tactacttaa acgccgaccg
     8281 ctggactccc aacgcgatcg ggtctctgtc cgaaattaac cgtgtccacc tcttggccca
     8341 gtacttcccg acggcctacg gcacggtggt tagcactgtg gcctcagtgc cgtccggcct
     8401 cgagccaagc gcaatggcgg gagtaccagg tgttggcagc caacagtccg aagtgaggac
     8461 aatcactgtt gaagagcaca acgcccgtct gaaggcagag gtggcagaga tgaaggcgcg
     8521 cctgcagacg ctggaagagc aggtgaagca gcagaacgcg ggagggggcc gttgaaaacg
     8581 aaacagtcga atttcgattc ggcgtggtgg ggcggcagag caccgggttt accgctcgtg
     8641 ctgatcggat tcctcttcgc gctctctctt tccgccttcg cttctgatca gcggaaggca
     8701 accggttcgc aactctcgcc ggtacaggag agcaagccct ccgaagcccg cacaaccgcg
     8761 ccgcttctgt tagagttgga gtgcctttcg gttcagggaa gcggagcgac gtttgtcacc
     8821 ggcggcgggc tgcagttggg gttttcggtg gcccagtcgg tggcgggcgc acagagcgcg
     8881 gcc
//
LOCUS       DBNGZD010000440         2725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1666486, whole genome shotgun sequence.
ACCESSION   DBNGZD010000440 DBNGZD010000000
VERSION     DBNGZD010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1666486"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="AC16GM_11540"
     CDS             complement(<1..588)
                     /locus_tag="AC16GM_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR2924340.1"
                     /translation="MKQPALDSAAALAGGILLGSAVNLSGVLLFLVTVGLVGLGFFAH
                     WHQRPRLATVTLLGVLVAAGMMRYGQVTRDFPLNHISHFVHLGKSLTVTARITDLPDV
                     FNDRTYLTVVVDSLSDGRKRVAASGKISVRLREPTSAFAYGDRIRFSGTIWPPPEAKN
                     PGAFDYQRYLQAQEIFGLVYLSSVNLVAVQRGSELR"
     gene            complement(590..877)
                     /locus_tag="AC16GM_11545"
     CDS             complement(590..877)
                     /locus_tag="AC16GM_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2924341.1"
                     /translation="MFYFAHFRLGALPWGTMGAGASRVLAAALLAGLIGWGTPRLFPY
                     FSAAHTVIGRFITVAILLSLFSLTYGLAGKLLRIPEWQQVSGLFLRLFRKQ"
     gene            complement(941..2185)
                     /gene="murJ"
                     /locus_tag="AC16GM_11550"
     CDS             complement(941..2185)
                     /gene="murJ"
                     /locus_tag="AC16GM_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR2924342.1"
                     /translation="MSTSAPRLDSGPAPLARNAAVVSLATVASRLFGLLREQTFAYLF
                     GAGLATDAFVAAFRIPNLLRDLFAEGVLGVAFVPVFTDSLTKKGKVEAFRVANLCASG
                     LVVAVSAVVIVGIVASPWIVHLIAPGFEKIPGKMELTALLTRIMFPFLLFIALAALWM
                     GMANSLGSFAIPAAAPILFNLGMIVAGFTLGSLFAEPVVAMALGVLVGGFGQWAIQWP
                     VVHRHGFRPAWELSFRDPELRRILVLMVPAVIALASTQVNIFVNTLVASLLPQGSVSY
                     LNYSFRLMHFPLGAFGIAVATVTLPLVSRLAAQGDRTGMLAAVTSSLRLVFFLTIPAT
                     VFLLVAGKPLVALLYQYGRFTRADTLATASALWFYALGLVFYASVRVLAQTFYAARLP
                     KIPVAIGALAVAFNIGLNLVLM"
     gene            2370..2636
                     /gene="rpsT"
                     /locus_tag="AC16GM_11555"
     CDS             2370..2636
                     /gene="rpsT"
                     /locus_tag="AC16GM_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR2924343.1"
                     /translation="MPRHRSPEKALRKSTRENLRNRIWKSKVKTAVKAVEAAPDAATA
                     QAQLRTAVSLLDKAARRKIIHPNAAARVKSHLAKSIRARVTRQP"
BASE COUNT          703 a          789 c          828 g          405 t
ORIGIN      
        1 acgcagctca gagcctcgct gtaccgcaac cagattcacc gaggaaaggt acaccagccc
       61 gaaaatttct tgggcctgga gatacctttg atagtcgaac gcacccgggt tctttgcctc
      121 aggcggcggc cagatggtgc cgctaaaccg tatccggtcg ccgtaagcga aagcagaagt
      181 cggttccctg aggcgaacag atattttgcc gctggcagca acccgttttc tgccgtcgga
      241 aagggaatcg actacgaccg tcaggtaggt gcgatcgttg aagacgtcgg gaagatcggt
      301 gatgcgtgcc gtgaccgtca gagattttcc aagatgaacg aagtggctga tgtggttcag
      361 cggaaagtcg cgggttactt gcccgtagcg catcatcccc gccgcaacca gaacacccaa
      421 gagggtgacc gttgccagcc gtgggcgctg gtgccagtgg gcaaaaaaac cgaggccgac
      481 cagtccgacg gtgaccaaaa agaggagcac tccggagagg ttcaccgcgg aacccaaaag
      541 aatccctccg gccagggccg ctgcgctgtc caaagccggc tgcttcatgc tactgctttc
      601 tgaacaaacg caggaaaaga ccggaaacct gctgccattc aggaatccgc aggagtttgc
      661 cggccaggcc gtaggtcagc gagaagagac tgagtaagat cgccacggtg atgaaacgac
      721 caatgacggt gtgggcagcc gaaaaatacg gaaaaagtcg gggtgtgccc cagccaatca
      781 agccggccaa tagcgcagcg gccaaaaccc ggctggcacc cgctcccatt gtcccccagg
      841 gaagtgcgcc cagccgaaag tgggcaaaat aaaacaaacc gcagacattc agagcagcgg
      901 aggctgaagt tgccagcgcc agcccgcgaa accccagagg ttacatgagc actaaattca
      961 aaccaatatt gaaggccacg gccagggctc caatcgctac cgggattttg ggcaacctgg
     1021 cggcgtagaa cgtttgcgcc agaacccgca ccgacgcata gaagactaaa ccgagcgcat
     1081 agaaccagag ggcgctggcg gtggcgagag tatcggcccg ggtgaagcgg ccgtactggt
     1141 acaagagcgc caccagaggt ttgccggcca ccaaaaggaa aaccgtggcc ggaatggtga
     1201 ggaaaaaaac caaccggagg gaactagtga cggcggcgag catcccggtc cggtcaccct
     1261 gcgctgctaa acgcgagact aaaggaagcg tcaccgtggc tacggcaatg ccgaaggcac
     1321 ccagaggaaa atgcatcagc cgaaaactgt agttgagata ggaaacgctc ccctggggta
     1381 aaagcgaggc cacaagcgta ttgacgaaaa tgttcacctg ggtcgaagcc agggcgatga
     1441 cggccggcac catcagaacg aggatgcggc gcagttccgg atcgcgaaag ctaagctccc
     1501 aggcgggacg aaacccgtgc cggtgcacca cgggccactg aatggcccac tgaccgaatc
     1561 ccccgaccaa aacgcctaag gccatggcga cgaccggctc cgcaaagagc gacccgagcg
     1621 tgaaacccgc cacaatcatc cccaggttaa agagaatcgg cgccgccgct ggtatcgcga
     1681 atgatcctaa cgagttggcc attcccatcc agagtgcggc taaggcgatg aacagaagaa
     1741 acgggaacat gatccgcgtg aggagcgcgg tcaattccat ctttccggga attttctcga
     1801 atccgggtgc aatcaggtgg acaatccagg gtgaagcgac tattcccacg atgaccacgg
     1861 ccgagacggc gacgaccaat ccactggcgc agagattcgc gacgcggaag gcttcgactt
     1921 tccctttttt ggtcagacta tcggtaaaga ccgggacgaa ggcgacgccc aggacgccct
     1981 ccgcaaacag gtcgcgcagc aaattgggaa tgcggaaggc cgccacaaag gcgtcggtgg
     2041 ccaggccggc cccgaagagg taggcaaagg tctgctcacg caacaggccg aagagccgcg
     2101 aggcgaccgt ggccagactg accacggcgg catttcgggc cagaggggct ggtcccgagt
     2161 caagccgagg ggcgctggtg ctcacggatc cggcacgacc cggcaaaccg cctcggcgaa
     2221 cctgttgttc acttcccggt catccgcttg tgcagagttc gtttgccgcc aaatccggag
     2281 gagcggaatt tgacggcccg cgcaaaagag ctatattcac tgcccaaaga ataagtaaat
     2341 gcctgagtcc cgaaatagag gaggaggagt tgccccgaca tcgttcgccc gagaaggcac
     2401 tgcggaagag cacaagagag aacctgcgca accgcatctg gaaatcgaaa gtgaaaacgg
     2461 cggtgaaagc ggtggaagcg gcccccgatg cggctacggc gcaggcgcag ttgcggacgg
     2521 ccgtgtcgct tctggacaaa gccgcccgtc ggaaaatcat ccacccgaat gccgccgccc
     2581 gggtcaaatc gcatctggcg aaatctatac gagcgcgcgt aacccggcaa ccgtagtcct
     2641 aaccgcagga ccggaaacaa cgctcgtccc gaggcatccc taaagagagt gcttccgtcc
     2701 ggaatggcat tattcttgta ggaaa
//
LOCUS       DBNGZD010000441         4641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05495
            SRR21617315_k127_1108718, whole genome shotgun sequence.
ACCESSION   DBNGZD010000441 DBNGZD010000000
VERSION     DBNGZD010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414903
            Sequence Read Archive: SRR21617315
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:58:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,315
            CDSs (total)                      :: 2,266
            Genes (coding)                    :: 2,253
            CDSs (with protein)               :: 2,253
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 1 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 4 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617315_k127_1108718"
                     /isolate="MAG05495"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.62 E"
                     /collection_date="2020-11-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16GM_11560"
     CDS             <1..335
                     /locus_tag="AC16GM_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HIR2924344.1"
                     /translation="MILDSSKYTGRLDKLLLQYRREGTRFGRVRNFADAPCFADSKTT
                     RLLQAADLIAHAIFRRYERSDAWLFDRIVSRFDKSEGIIHGLTHMIAKYRECPCPACI
                     SRISRPRQ"
     gene            complement(520..1104)
                     /locus_tag="AC16GM_11565"
     CDS             complement(520..1104)
                     /locus_tag="AC16GM_11565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2924345.1"
                     /translation="MKNPPPITYHPFTSSLWPDLERLFGPNGACAGCWCMWWRIPHGG
                     KLWREMQGQKAKRSLKQLVRKRKAFGVLAYAGDEPVGWCTFGPRIDFPRLQRVRAYTR
                     DDIEKVWSIPCFFIHRNWRSQGLARGLLTEAIRLARKKGAKIVEGYPVPLTRAGKKLP
                     AAWSYTGPLGIFEEQGFRIVQRVSRSAPLVRKKL"
     gene            complement(1279..2652)
                     /locus_tag="AC16GM_11570"
     CDS             complement(1279..2652)
                     /locus_tag="AC16GM_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2924346.1"
                     /translation="MAEFLNPMRKHPILVVDDEEPQRTLLAGYLAKQGYSATACSSGE
                     QALEQASRHFFSSALVDLKMPGIDGVELTRRLREHNPELQVIVITAFGTVETAVEAMK
                     AGAFHYITKPIDLEVLSATLTQALEKQTLLSENRVLREELEKIAPDETIVGNSAGMRQ
                     VLATVARVAPTDSTVLIRGESGTGKELIARALHRMSPRVSKRFVAINCAALPESLLES
                     ELFGHEKGAFTGALKQKPGRFELADGGTLFLDEIGDLSPALQAKLLRVLETHEIERLG
                     GTAALPVDVRVICATHQNLEQRIKEKTFREDLFFRLNVIEIPIPPLRERRDDIPLLVQ
                     HFLEKFQKKTGKKISGLTPEAKQLLLNYAWPGNVRELENLLERACVLARGEVLGIADF
                     PPLTGKNVREKTDESEPVSLKDLERQQIVKMLQRYDGNLGQTAQALGIHRNTLRMKMR
                     EYGITAP"
     gene            complement(2723..>4641)
                     /locus_tag="AC16GM_11575"
     CDS             complement(2723..>4641)
                     /locus_tag="AC16GM_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2924347.1"
                     /translation="RRLGIALIESLTLASQNTVRSNALVEALIDDRLTDLAGLIVQLA
                     HAGKYSPALLKQIADQQGVERIDLFGAKKNLLTSTTSDEPFYLQQADVSALVDSVLWG
                     YKKSIIVTLQPDPLHTEEHLALVAAAPGLAKAMVIVTSLEFLGDFRREIGIGYLIQKI
                     GQESGIEYVVLQNPEGIVLASRKVARMPKIESDLFLTEALGGDSVRTRLTDFDGHEVL
                     EVVRPFRSQEIPGGLFRIGLSLEGLSQTAANFRRQVLLITLVIFLIGLLVVGLGVVNR
                     NYAALERSFLDTKGLTQTVLEAMEGAVVAIDAKGLISVFNPKAEKLFDRRAGEVTGKK
                     YRELFPEDQFLLHQTVMTGKSTIGTERDFALSDGKRRMLVSTARLADDTGTITGAVAV
                     LSDVTQLRKLEEEAHRAERLSALGTMAAGVAHEIRNPLNAISIAAQRLKTEFMPKEEN
                     DQYQKLTTTITTEVQRLNQTVNQFLSLARAQAVNKQKTNLAELLNEVVAVAKMEAEPK
                     KIGIQTEWAKNLPVALDKNEMKQALLNICLNGIQAMDTGGTLAVKAGVDSSIDRQMKI
                     EITDSGPGIAPESLSRLFQPYFTTKPGGTGLGLAIVQRIVQDHQGKIEVESQVGKGTR
                     FVVILPVEERERAF"
BASE COUNT          927 a         1303 c         1338 g         1073 t
ORIGIN      
        1 taatgattct agattcatct aagtatactg ggcgtttaga caaattactg ttgcaatatc
       61 gtcgtgaggg cacccgtttt ggtagagtaa gaaacttcgc cgatgctcct tgctttgcag
      121 actcaaaaac aacgaggctt cttcaggctg ctgacttgat cgcacatgcc atctttaggc
      181 gatatgaacg ttccgatgct tggttatttg ataggatagt ttcccgattt gataaatctg
      241 aaggaataat tcatggcctg actcacatga tcgcgaaata tcgtgaatgt ccatgtcctg
      301 cttgtattag tagaatttcg cggccacgtc agtaggtagc aaatttttag accgccgtta
      361 tttcttcatc tcgagtttca gcgcagcgga gttgatgcag tagtctgcct gaggcggact
      421 ggcgggggca gtcaggatgg gcggggctga gagccccgcc tacgcgagaa gaaattctct
      481 cctcatcgcg taggcggcac tcttagtgcc gccatttcct caaagcttct ttctcaccaa
      541 aggtgcggag cgtgagaccc gctggacaat tctaaatccc tgctcttcga atattcccag
      601 cgggccggtg taagaccagg cggcgggcaa ctttttgccc gctcgggtca aaggcaccgg
      661 gtagccctcg acgatcttag cacctttctt tctggcaagg cgaattgctt ctgtaaggag
      721 cccgcgcgcc aggccttggc tgcgccagtt acggtgaatg aaaaagcagg ggatcgacca
      781 gactttttcg atatcgtctc gcgtatacgc tcgaactctc tgcaagcgag ggaaatctat
      841 tcgcgggccg aaagtgcacc agccgacagg ttcatcaccg gcgtaggcca gaacgccgaa
      901 cgctttgcgc ttgcgaacta attgtttgag cgaacgcttg gctttttgtc cttgcatctc
      961 gcgccacagt ttccccccgt ggggaatgcg ccaccacatg caccagcagc cggcgcaggc
     1021 gccgttcggc ccaaagaggc gctcaagatc aggccagagg gaggaggtga acgggtggta
     1081 ggtgataggt ggtggatttt tcatttagtt ctccttttga cctcacccca accctctccc
     1141 cttcggctgg ctcagggtgg tgagcaaagt cgaaccacta caggagaggg agatatcctt
     1201 actccaaaga taggcagttc cctcccctgt agggtcgaag actcgtaccg aggagggcag
     1261 ggtggggtca aaaatgcttc acggggctgt aatcccatac tccctcatct tcatccgaag
     1321 cgtgttgcgg tgaatgccca gagcctgcgc agtctgaccc aggtttccgt cgtagcgttg
     1381 cagcatcttc acgatctgct ggcgttccag gtctttcagc gacaccggct ccgactcatc
     1441 agttttctcc cggacgtttt ttccagtcaa gggtgggaag tcggcgatgc cgagaacctc
     1501 tccacgcgcg aggacgcagg cgcgttccaa aagattttcc aattcgcgca cgttgccggg
     1561 ccaggcgtag ttcagaagga gctgcttcgc ttccggggtc aggccggaaa ttttcttgcc
     1621 ggttttcttc tggaatttct ccagaaagtg ctggactaaa agggggatgt cgtcccggcg
     1681 ctcccggagc ggaggaatgg ggatttcaat cacgttgaga cggaagaaga ggtcttcgcg
     1741 gaacgttttc tctttgatcc gctgctccag gttctgatgc gtggcgcaaa tcacccgcac
     1801 gtcaaccggc aaagcggcgg tgccgcccag ccgttcaatc tcatgcgtct ccaacacgcg
     1861 caaaagtttt gcctgcaagg cgggggagag gtcgccgatt tcgtccaaga acagcgttcc
     1921 gccatcggct aactcaaagc ggcccggttt ttgtttcaac gcgccggtga aagcgccctt
     1981 ctcgtggccg aagagttccg attctaaaag cgactccggc agggccgcgc aattgatcgc
     2041 cacgaagcgc tttgagacgc ggggcgacat ccggtggagg gcgcgggcga tcagttcctt
     2101 gccggtgccg ctttcgccgc gaatcaggac ggtggagtcg gtgggcgcca cgcgggccac
     2161 ggtcgccagc acctggcgca ttcccgcgct gttaccgaca atggtttcat ccggcgcaat
     2221 tttctccaac tcttcccgca aaactcgatt ttcgctcaaa agcgtctgct tttccagcgc
     2281 ctgcgtgagg gtggcggaga ggacttcgag gtcaatcggc ttggtgatgt agtggaaggc
     2341 gccggccttc atcgcctcca cagcagtctc gacggtgccg aaagcggtga tgacgatcac
     2401 ctgcagttcc ggattatgtt cgcgcaggcg gcgggtgagt tccacgccgt cgatgccggg
     2461 cattttcaaa tcgactaacg ccgaggagaa aaaatgccgt gaggcctgct ccagggcctg
     2521 ttcgcctgaa ctgcaggcgg tcgcgctgta gccctgcttg gccagatagc cggccaaaag
     2581 ggtgcgctgg ggctcttcgt cgtcaacgac taagatcggg tgtttgcgca tggggttcag
     2641 gaactcagcc aatgacccct ccctgccctc ccctcaaagg ggagggatgc tgagagatcg
     2701 attttgcccg ttcacccgga gttcagaagg ctctttcgcg ttcctccaca ggtagaataa
     2761 ccacgaaacg cgtaccctta ccaacttgac tttcaacttc gatttttccc tggtggtctt
     2821 gaacgattcg ctgcacaatg gccagaccca ggccggtgcc gccgggtttg gtggtgaaat
     2881 acggctgaaa cagacgactc aaactttccg gcgcaatgcc cgggccggaa tcggtgattt
     2941 cgattttcat ctgacggtcg atggaagaat caaccccggc cttgaccgcg agcgtgccgc
     3001 cggtgtccat cgcctgaatc ccattgagac agatgttcaa aagcgcctgt ttcatttcgt
     3061 ttttgtccag tgctacaggg agattttttg cccactcagt ctgtatgccg atctttttgg
     3121 gctcggcttc catcttcgcc acagcgacaa cttcgttcaa cagttccgcc aagttggttt
     3181 tctgcttgtt gaccgcctgc gcgcgggcca ggctcaagaa ctggttcacg gtttgattca
     3241 accgctggac ttcggtcgtg atggtggtgg tcaacttctg gtactggtcg ttttcctctt
     3301 tcggcatgaa ctcggttttc aggcgctggg ccgcgatgct gatggcgttc agaggatttc
     3361 tgatttcgtg ggcgacaccg gcggccattg tccccaaagc ggagagccgt tcagcacgat
     3421 gcgcttcttc ctctaatttc cgcaactggg tcacatcgga gaggaccgct accgcgccgg
     3481 taatggtgcc ggtgtcgtca gcgagccggg cggtgctgac cagcatgcgg cgtttgccgt
     3541 cgctcaaggc aaaatcgcgc tcggtgccga tggtactctt gccagtcatc accgtctggt
     3601 ggagcaaaaa ctggtcttcc ggaaaaagtt ctctatactt cttgccagtc acctcacccg
     3661 cccgccggtc aaaaagtttt tcggctttag gattgaagac ggagatcaaa cctttcgcgt
     3721 cgatggccac caccgcaccc tccattgctt cgagcaccgt ctgagtcagc cctttagtat
     3781 ccaggaaaga gcgctccagc gcggcgtagt tgcggttcac tacgcccagg ccgacgacta
     3841 aaagtccgat caaaaaaatc accagcgtaa tgagcaacac ctgacggcgg aaattcgcgg
     3901 cggtctggga gagtccttcg agcgaaagcc cgatacggaa gagcccgccg ggaatctcct
     3961 gtgagcgaaa gggccgcacc acttccagca cttcgtggcc gtcaaaatcg gtgaggcgtg
     4021 tccggacgct gtcgccgccc agggcctcgg tcaaaaacag atcgctttca attttgggca
     4081 tccgcgccac tttgcgcgag gcgagcacaa tcccttccgg attctgcagg acgacatatt
     4141 caatcccgct ctcctggccg attttctgaa ttaagtagcc gatgccgatt tcgcggcgga
     4201 agtcgcccag gaattcgagc gaggtgacaa tcaccatcgc tttggccaag cccggagcgg
     4261 cagctaccag cgccaggtgc tcttcggtgt ggagcgggtc gggttgcagg gtcacgatga
     4321 ttgatttctt gtatccccag aggaccgaat cgaccagggc gctgacgtcc gcttgctgga
     4381 gatagaacgg ttcgtctgat gtcgtgctgg tgaggagatt tttcttggcg ccgaagaggt
     4441 ctatccgctc cacgccctgt tggtcggcga tttgtttgag gagcgcgggg gagtacttgc
     4501 cggcgtgcgc cagttgcaca atcagacccg ccaggtcggt gagccggtcg tcgattaacg
     4561 cttccaccag cgcgttcgac cggacggtgt tctggctggc cagagtcagg ctttcgatta
     4621 gggcaattcc cagacggcgc a
//
